LOCUS       DAZAID010000001        77327 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000001 DAZAID010000000
VERSION     DAZAID010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.001"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..823)
                     /locus_tag="ACG9BE_000001"
     CDS             complement(<1..823)
                     /locus_tag="ACG9BE_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFN3030792.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFK"
     gene            complement(894..1355)
                     /locus_tag="ACG9BE_000002"
     CDS             complement(894..1355)
                     /locus_tag="ACG9BE_000002"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFN3030793.1"
                     /translation="MFQILKLSKNEIRHSNFLAFLFAPNETHNLSDEFFKMFLKRYID
                     SNDDTKAAINYFDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQ
                     LNKYQIYIERNYSNYKKIFVFLTPDGNEPTNPSWGIITYKNIVEESVKYFV"
     gene            complement(1319..1444)
                     /locus_tag="ACG9BE_000003"
     CDS             complement(1319..1444)
                     /locus_tag="ACG9BE_000003"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3030794.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            complement(1647..2126)
                     /gene="rlmH"
                     /locus_tag="ACG9BE_000004"
     CDS             complement(1647..2126)
                     /gene="rlmH"
                     /locus_tag="ACG9BE_000004"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFN3030795.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2554..3843
                     /locus_tag="ACG9BE_000005"
     CDS             2554..3843
                     /locus_tag="ACG9BE_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFN3030796.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(3882..4487)
                     /gene="ytpR"
                     /locus_tag="ACG9BE_000006"
     CDS             complement(3882..4487)
                     /gene="ytpR"
                     /locus_tag="ACG9BE_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFN3030797.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(4692..5156)
                     /locus_tag="ACG9BE_000007"
     CDS             complement(4692..5156)
                     /locus_tag="ACG9BE_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN3030798.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5178..5498)
                     /locus_tag="ACG9BE_000008"
     CDS             complement(5178..5498)
                     /locus_tag="ACG9BE_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN3030799.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(5642..6721)
                     /gene="pepA"
                     /locus_tag="ACG9BE_000009"
     CDS             complement(5642..6721)
                     /gene="pepA"
                     /locus_tag="ACG9BE_000009"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFN3030800.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            6921..7271
                     /locus_tag="ACG9BE_000010"
     CDS             6921..7271
                     /locus_tag="ACG9BE_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFN3030801.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7494..8441
                     /locus_tag="ACG9BE_000011"
     CDS             7494..8441
                     /locus_tag="ACG9BE_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN3030802.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8498..9916)
                     /gene="pepV"
                     /locus_tag="ACG9BE_000012"
     CDS             complement(8498..9916)
                     /gene="pepV"
                     /locus_tag="ACG9BE_000012"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFN3030803.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10065..10910
                     /locus_tag="ACG9BE_000013"
     CDS             10065..10910
                     /locus_tag="ACG9BE_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFN3030804.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            10914..11354
                     /locus_tag="ACG9BE_000014"
     CDS             10914..11354
                     /locus_tag="ACG9BE_000014"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFN3030805.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11404..11598
                     /locus_tag="ACG9BE_000015"
     CDS             11404..11598
                     /locus_tag="ACG9BE_000015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3030806.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            11734..12411
                     /locus_tag="ACG9BE_000016"
     CDS             11734..12411
                     /locus_tag="ACG9BE_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3030807.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(12819..13508)
                     /locus_tag="ACG9BE_000017"
     CDS             complement(12819..13508)
                     /locus_tag="ACG9BE_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3030808.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13483..15612)
                     /locus_tag="ACG9BE_000018"
     CDS             complement(13483..15612)
                     /locus_tag="ACG9BE_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFN3030809.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(15660..16997)
                     /locus_tag="ACG9BE_000019"
     CDS             complement(15660..16997)
                     /locus_tag="ACG9BE_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFN3030810.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17249..17608)
                     /gene="rplT"
                     /locus_tag="ACG9BE_000020"
     CDS             complement(17249..17608)
                     /gene="rplT"
                     /locus_tag="ACG9BE_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFN3030811.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(17661..17861)
                     /gene="rpmI"
                     /locus_tag="ACG9BE_000021"
     CDS             complement(17661..17861)
                     /gene="rpmI"
                     /locus_tag="ACG9BE_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFN3030812.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(17933..18457)
                     /gene="infC"
                     /locus_tag="ACG9BE_000022"
     CDS             complement(17933..18457)
                     /gene="infC"
                     /locus_tag="ACG9BE_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFN3030813.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18501..18626)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            18777..19409
                     /locus_tag="ACG9BE_000023"
     CDS             18777..19409
                     /locus_tag="ACG9BE_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN3030814.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19411..20316)
                     /locus_tag="ACG9BE_000024"
     CDS             complement(19411..20316)
                     /locus_tag="ACG9BE_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN3030815.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20401..21060)
                     /locus_tag="ACG9BE_000025"
     CDS             complement(20401..21060)
                     /locus_tag="ACG9BE_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN3030816.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21376..22320
                     /gene="mvk"
                     /locus_tag="ACG9BE_000026"
     CDS             21376..22320
                     /gene="mvk"
                     /locus_tag="ACG9BE_000026"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFN3030817.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22313..23290
                     /gene="mvaD"
                     /locus_tag="ACG9BE_000027"
     CDS             22313..23290
                     /gene="mvaD"
                     /locus_tag="ACG9BE_000027"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFN3030818.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23303..24388
                     /locus_tag="ACG9BE_000028"
     CDS             23303..24388
                     /locus_tag="ACG9BE_000028"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFN3030819.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24385..25449
                     /gene="fni"
                     /locus_tag="ACG9BE_000029"
     CDS             24385..25449
                     /gene="fni"
                     /locus_tag="ACG9BE_000029"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFN3030820.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(25698..26501)
                     /locus_tag="ACG9BE_000030"
     CDS             complement(25698..26501)
                     /locus_tag="ACG9BE_000030"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFN3030821.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26521..27363)
                     /gene="kduI"
                     /locus_tag="ACG9BE_000031"
     CDS             complement(26521..27363)
                     /gene="kduI"
                     /locus_tag="ACG9BE_000031"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFN3030822.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27379..28386)
                     /locus_tag="ACG9BE_000032"
     CDS             complement(27379..28386)
                     /locus_tag="ACG9BE_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFN3030823.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28393..29037)
                     /locus_tag="ACG9BE_000033"
     CDS             complement(28393..29037)
                     /locus_tag="ACG9BE_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFN3030824.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29137..29895)
                     /locus_tag="ACG9BE_000034"
     CDS             complement(29137..29895)
                     /locus_tag="ACG9BE_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFN3030825.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30022..31173)
                     /gene="fucO"
                     /locus_tag="ACG9BE_000035"
     CDS             complement(30022..31173)
                     /gene="fucO"
                     /locus_tag="ACG9BE_000035"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFN3030826.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31255..31566)
                     /gene="rhaM"
                     /locus_tag="ACG9BE_000036"
     CDS             complement(31255..31566)
                     /gene="rhaM"
                     /locus_tag="ACG9BE_000036"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFN3030827.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31568..32413)
                     /gene="rhaD"
                     /locus_tag="ACG9BE_000037"
     CDS             complement(31568..32413)
                     /gene="rhaD"
                     /locus_tag="ACG9BE_000037"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFN3030828.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32427..33683)
                     /gene="rhaA"
                     /locus_tag="ACG9BE_000038"
     CDS             complement(32427..33683)
                     /gene="rhaA"
                     /locus_tag="ACG9BE_000038"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFN3030829.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(33680..35140)
                     /gene="rhaB"
                     /locus_tag="ACG9BE_000039"
     CDS             complement(33680..35140)
                     /gene="rhaB"
                     /locus_tag="ACG9BE_000039"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFN3030830.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35293..36162
                     /locus_tag="ACG9BE_000040"
     CDS             35293..36162
                     /locus_tag="ACG9BE_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN3030831.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36288..37187)
                     /locus_tag="ACG9BE_000041"
     CDS             complement(36288..37187)
                     /locus_tag="ACG9BE_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3030832.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37352..38176)
                     /locus_tag="ACG9BE_000042"
     CDS             complement(37352..38176)
                     /locus_tag="ACG9BE_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN3030833.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38187..39482)
                     /locus_tag="ACG9BE_000043"
     CDS             complement(38187..39482)
                     /locus_tag="ACG9BE_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFN3030834.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(39498..39977)
                     /locus_tag="ACG9BE_000044"
     CDS             complement(39498..39977)
                     /locus_tag="ACG9BE_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFN3030835.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(39983..41011)
                     /locus_tag="ACG9BE_000045"
     CDS             complement(39983..41011)
                     /locus_tag="ACG9BE_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFN3030836.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41028..42134)
                     /locus_tag="ACG9BE_000046"
     CDS             complement(41028..42134)
                     /locus_tag="ACG9BE_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN3030837.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42363..44960)
                     /gene="adhE"
                     /locus_tag="ACG9BE_000047"
                     /gene_synonym="adhC"
     CDS             complement(42363..44960)
                     /gene="adhE"
                     /locus_tag="ACG9BE_000047"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFN3030838.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(45265..45645)
                     /gene="yajC"
                     /locus_tag="ACG9BE_000048"
     CDS             complement(45265..45645)
                     /gene="yajC"
                     /locus_tag="ACG9BE_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFN3030839.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(45742..46887)
                     /gene="tgt"
                     /locus_tag="ACG9BE_000049"
     CDS             complement(45742..46887)
                     /gene="tgt"
                     /locus_tag="ACG9BE_000049"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFN3030840.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            47105..47803
                     /locus_tag="ACG9BE_000050"
     CDS             47105..47803
                     /locus_tag="ACG9BE_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFN3030841.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(47971..50610)
                     /locus_tag="ACG9BE_000051"
     CDS             complement(47971..50610)
                     /locus_tag="ACG9BE_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN3030842.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(51229..52260)
                     /gene="queA"
                     /locus_tag="ACG9BE_000052"
     CDS             complement(51229..52260)
                     /gene="queA"
                     /locus_tag="ACG9BE_000052"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFN3030843.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            52453..53628
                     /locus_tag="ACG9BE_000053"
     CDS             52453..53628
                     /locus_tag="ACG9BE_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN3030844.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            53632..54273
                     /locus_tag="ACG9BE_000054"
     CDS             53632..54273
                     /locus_tag="ACG9BE_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3030845.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            54270..55187
                     /locus_tag="ACG9BE_000055"
     CDS             54270..55187
                     /locus_tag="ACG9BE_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3030846.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            55184..55855
                     /locus_tag="ACG9BE_000056"
     CDS             55184..55855
                     /locus_tag="ACG9BE_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3030847.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(56396..56884)
                     /locus_tag="ACG9BE_000057"
     CDS             complement(56396..56884)
                     /locus_tag="ACG9BE_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFN3030848.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(57085..57351)
                     /locus_tag="ACG9BE_000058"
     CDS             complement(57085..57351)
                     /locus_tag="ACG9BE_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN3030849.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(57351..57614)
                     /locus_tag="ACG9BE_000059"
     CDS             complement(57351..57614)
                     /locus_tag="ACG9BE_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN3030850.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            58135..58275
                     /locus_tag="ACG9BE_000060"
                     /pseudo
     CDS             58135..58275
                     /locus_tag="ACG9BE_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(58417..60240)
                     /locus_tag="ACG9BE_000061"
     CDS             complement(58417..60240)
                     /locus_tag="ACG9BE_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN3030851.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(60838..63543)
                     /locus_tag="ACG9BE_000062"
     CDS             complement(60838..63543)
                     /locus_tag="ACG9BE_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFN3030852.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(63849..64796)
                     /locus_tag="ACG9BE_000063"
     CDS             complement(63849..64796)
                     /locus_tag="ACG9BE_000063"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFN3030853.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            64986..65255
                     /gene="rpsN"
                     /locus_tag="ACG9BE_000064"
     CDS             64986..65255
                     /gene="rpsN"
                     /locus_tag="ACG9BE_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFN3030854.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(65431..65793)
                     /locus_tag="ACG9BE_000065"
     CDS             complement(65431..65793)
                     /locus_tag="ACG9BE_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN3030855.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(65804..66925)
                     /gene="alr"
                     /locus_tag="ACG9BE_000066"
     CDS             complement(65804..66925)
                     /gene="alr"
                     /locus_tag="ACG9BE_000066"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFN3030856.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(66941..67291)
                     /gene="acpS"
                     /locus_tag="ACG9BE_000067"
     CDS             complement(66941..67291)
                     /gene="acpS"
                     /locus_tag="ACG9BE_000067"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFN3030857.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(67421..68932)
                     /locus_tag="ACG9BE_000068"
     CDS             complement(67421..68932)
                     /locus_tag="ACG9BE_000068"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN3030858.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(69254..70606)
                     /gene="murF"
                     /locus_tag="ACG9BE_000069"
     CDS             complement(69254..70606)
                     /gene="murF"
                     /locus_tag="ACG9BE_000069"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFN3030859.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(70759..71835)
                     /locus_tag="ACG9BE_000070"
     CDS             complement(70759..71835)
                     /locus_tag="ACG9BE_000070"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFN3030860.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            72031..73776
                     /locus_tag="ACG9BE_000071"
     CDS             72031..73776
                     /locus_tag="ACG9BE_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFN3030861.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(74056..74493)
                     /locus_tag="ACG9BE_000072"
     CDS             complement(74056..74493)
                     /locus_tag="ACG9BE_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFN3030862.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(74477..75850)
                     /locus_tag="ACG9BE_000073"
     CDS             complement(74477..75850)
                     /locus_tag="ACG9BE_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3030863.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(76172..76660)
                     /locus_tag="ACG9BE_000074"
     CDS             complement(76172..76660)
                     /locus_tag="ACG9BE_000074"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3030864.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(76682..77284)
                     /locus_tag="ACG9BE_000075"
     CDS             complement(76682..77284)
                     /locus_tag="ACG9BE_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN3030865.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        22216 a        16480 c        14093 g        24538 t
ORIGIN      
        1 atttgaaatc gctacagatt tcttgtcgat cactaacacg aaccttatga gcgatatttc
       61 tagatatatg gacacaacat tgttgaaatt gagcatttgg ataaacacta tgaatactat
      121 cgtcaatacc acttaaacca tctgttacga ctaacaaaac ctcttctaaa ccacggtctt
      181 ttagatcttg aagaatctct ttccaaacat aagcagattc agttggagct atagtaaatc
      241 ctagaacctc tttagttcca tccaacctga taccaattac tatatagaca gcttcttttg
      301 atacggtttg tctttttaag ggaatatgag tagcatccat aaaaatgact gagtatttag
      361 cttctaaagt tctttcttta aatgccgaaa catcttcaga cacaatttta ctcatatttg
      421 aaatagtttg tggtttgtaa taatgaccat acattttttc gattagatca gagatttcgg
      481 acatagtaat tcctttttga actagctgaa taatagtggt ttctaaagag tcattggttc
      541 ttttataagc aggtaaagtt tgttgagaaa agtctccatt cctatctcta ggtattacta
      601 aattcaattc accgtactct gttttaaaag agcgcgaata attcccatta cgggaattac
      661 ctgaattaaa tccagctcta tcatactttt catagtcaag aaataccgtt aattcagctt
      721 gtaataaagc gttaatagcc aattctaggt gacgacgaaa caaatcatcc aaatcacctt
      781 tattgattag tatttccata atttctgtag taaaatcgtt catatggaag tcctcttttc
      841 tgtgaatgtg ttgtggtaac tctattctac agaagggact tccttttttc tatttacaca
      901 aaatatttta cactctcttc aacgatattc ttgtaagtaa ttatgcccca actaggattt
      961 gtgggttcat ttccatctgg agttaaaaaa acaaaaattt ttttatagtt agaataattg
     1021 cgttctatat atatttggta tttattaagt tgccctccag actccgaaga cagaatttta
     1081 ttttcgatgc acaccactat tttatttttt tccgatacca ataaaatatc aatattctta
     1141 ttttctctgt aaacaattaa atcttcatag ctattaagta aagcatcaaa ataattgata
     1201 gcagctttgg tatcatcatt agaatctata taccttttca aaaacatttt aaaaaactca
     1261 tcagataaat tgtgggtttc atttggagca aacaaaaaag ctaaaaaatt actatgtctt
     1321 atttcatttt tagatagctt aagtatctga aatatatttg ttttcccagt ccatttagat
     1381 agttttcttc aatatcatcc atatcctcta atagattttt taaacacttt ttttcatcta
     1441 tcatgttttt tctccttgcc attattctaa ctaaaatttt acgtatttgt ttctatgtct
     1501 acattatctt tttaacagta tctatttttc ttttgcaact tacttggcta ccaaagcaaa
     1561 taaaaaagtt atttataaat ttctccctac cactacctat tgctattatt taaaataggg
     1621 ataatttttt ctcttaaaaa atatcattat ttgtggtatg gagcccccgc attgatccgg
     1681 aaggcacggt agatttgctc gacaaggatc agacgcatca attgatgtgg atacgtcatt
     1741 tttccaaaag agatctgagc attgcttctt ttcatgacgg cttcgctgag tcctaaagag
     1801 ccgccgataa caaaaacaag gtggcttttt ccttggatac ctaattggtc gatttgttta
     1861 gcaaaagatt cacttgtagg atttttccct tcaattgcta aggcatagac gtattcttgt
     1921 tctttgattt tcgctaagat ccgttcccct tctttttctt ttacttgtct catttgcgct
     1981 tcgcttaaat tctccggggc tttttcgtca ggaacttcga tcaattcgat ttttgcatag
     2041 gcattcagcc gtttgacata ctcattgata ccttggatca ggtatttttc ttttaatttt
     2101 cctactgaga taattttgat attcatgttt tctctccttt ggtcttcatg ctcactttgt
     2161 ttattataat caattttttc gtctatatgt ttattttggc tatttttgtt aaaaagattt
     2221 ccccaacagt tatgcacaac atccacaggt ttatccacag aaaacagtgt tattttacct
     2281 tatttcatca cattcattag gcaaacatac gttttataag aatttatcca ctttatacac
     2341 aggctgtgga tatgttttat cccttatttt tccaatccta tccgcgtagt tttacacagt
     2401 atctgtggaa aaagaaaatc aaatattttc atctgaaaac aatgattttt cctaaagttt
     2461 ttcataaaaa gttcacagtc tcaaagtaga ctaaagtcaa caaataagac tatactaaca
     2521 gtagataaat gaatgtaatt ggaggaggaa cacatggata gaaaaaatgt gacaccaaag
     2581 atgaaaaaaa gtaaaaataa tagcctctgg cgtaagttag gtctggggct tgttggaggg
     2641 atcgttggag gactactgac agctggcatt ttttatgcag cgatgggcac tggcaacaat
     2701 agttctactg ctacaagcgg caatcagaat tctgccggtg aaacagttgt ggaaaatgtc
     2761 aaagtcaatg tagattctga tatcaccaaa gcggtagata aagttcaaga cgctgtcgtt
     2821 tctgttatca acttacaaag tcaaaaccaa tcaagcggtt ttggcggact attcgggcag
     2881 caagaagaaa gtagcagctc agatgatagt aatttggaag cttatagtga aggaagcggc
     2941 gtgatctaca aaaaagacgg gaatactgct tatgtagtca cgaataatca cgtagtagat
     3001 ggccagcaag gtcttgaagt cttgatgaaa gacggtacga aggtaaaagc tgaattagtt
     3061 ggaacagatg cttattcaga tttagcggtg ttgaagatca attcagacaa agtcgaaaca
     3121 gttgcctctt ttggtgactc aagtgctctg aaggttggtg agcctgcgat tgcgattggt
     3181 tccccactag gatctgaata tgcaaattct gtcacttcag gaatcatttc ttccttgaac
     3241 cgacaagtga caagtacgaa tgaatcgaac gaaacagtca atatcaatgc gatccaaaca
     3301 gatgcagcaa tcaaccctgg aaattctggt ggtcctttag ttaatatcga aggacaagtt
     3361 atcggtatca actcaagtaa gatcgcaagt acttctgaat ctacttctaa tgtaagcgta
     3421 gaaggaatgg gatttgctat tccaagtaac gatgtagtga atatcatcaa tcaactggaa
     3481 aaagacggaa aagtgacacg tccagctcta ggtatcacaa tggtcgactt atctgccgtt
     3541 tctactcagc aacaagaaca aatcttgaag attccttcat ctgtaactaa cggtgtcatc
     3601 gtgacaaatg tccaaacagc tacaccggct gaaaaagctg gattgaaaca atacgacgtc
     3661 attactaaga tcgatgacaa agaagtttct tctggtgtag aacttcaatc tgctttgtat
     3721 aagaaaaaag tgggagattc tgtcaaaatc acttactatg aaggctcgaa agaaaaaaca
     3781 actactgttg aattgacgat cgatcaatcg gcattaaaac aaaatcaaaa tagtgaaaat
     3841 taataaaaaa gaggttgtga caaagacttg tcacaacctc tttattttgg aaatgcttct
     3901 ccgactacgg catcctcagg caatactaag attccttttt ttgctggagc atttggcaaa
     3961 tgcaattcac gagcggaaca gatcattcca tagctatcta cccctcgcaa agttcctggc
     4021 cagatcatca ttccatctgg catcatagct cctggttttg caacgacaac tttttgtcca
     4081 gcttcgatat tgggtgcacc gcaaacgatt tgcaatactg taccattgtc tacttctgtc
     4141 tcagtaatcg ataaatggtc agaatcagga tgtgggacgc atgtttttac gtacccgaca
     4201 acgatcttcg gttcatgatc agcttcaagc tgttcttcga atcctgcttc tttgatcaat
     4261 cgattcaatt tcgctaattc ctcatctgtt aagcttactt ggccgttccc ttcgattgtt
     4321 agatgatcgg agatatggaa aaagttccaa gcaacaactt gaccttgttc atctttgatc
     4381 cgagcaacat tccatttgcg ttcaacagtg ttttctgttc ctttatcgtc ggctataata
     4441 accatcaagg tgtctccgac gtgtgctttg ttataagcaa aaatcatgtt ctttcctcca
     4501 ttatcctttg ctgagattta tcttaacaaa aaattgcatt cactgcatga aatttttttc
     4561 atgctttatt tccgaagaag cctcttctgg aacaccattt attcgtaaca agggactgtg
     4621 acaagacttt tgtcacagtc cctttactgt tttagttaat tgattaaaaa ggtcgtacaa
     4681 gttccacttg cttaattact tggctgttct tcttcgtcag aaacgatcaa gacatcacaa
     4741 ggtgaatgac gtatcacgta gctggctaca cttcctgtca taaagcgttc gactgcattt
     4801 agacctgact gcccaaccat gattagatcg atctcatatt tttttggcaa tttgactgcc
     4861 atggattcac gaacagaacc atatgcaata attccttcta tatttgtgta atcaacagat
     4921 ttcccatatt ctttacattc ttcgatcatt tccttggctg tttcagtttg ctgatcgact
     4981 agattttgat caataggtgc atagcccata aagttataaa attgctgatc gattacgttt
     5041 gctacatata ctttcccatt attacggcga gccacttcta ccgctttttt gaaagcagca
     5101 agtgcttgtt cactaccgtc gatacctact aaaatattct tatattcttg attcatcttg
     5161 acccctccta acagatgtca gccttttgtt acactttcta ggaacgattc gatttcctgt
     5221 tttgttttac gatctttatt cactagacga cctaattctt ttccgtcttg gatcacgaca
     5281 aaacttggga taccaaaaat gttccattca gcggctacat ctatgtactt gtcacggtct
     5341 acttcaataa attgccaatc agaaaagtca ttttcaattt caggcatttg cggtttgata
     5401 aaacggcagt ctccgcacca gccggcagta aagaaaaata cactctttcc agtttctacg
     5461 tagcctgcta attcttctaa actatttggg ataatcattc tttgtcctct actttctgtt
     5521 tatgtatttt tcaattaaat tatacacctt tctttcgaac taacaaacat tgatcctctt
     5581 tttataaaaa aaatctggca aaaagcaatc ttttgccaga ttttatagtt tcactatcgc
     5641 attattttcc gtatacgatc gtatttactg ttgatttatc caaacctttc agtgtttgaa
     5701 tcaacatttc tcgtgcagct tcaaaatcgc gaatactgaa catcgtttga tgcgtatgga
     5761 tataacgtcc cactacgcca atcactgtac ttgggattcc ttcgttttgt gtatgagcag
     5821 cacctgcatc tgtaccgcct tttgaaacaa agtattgata agggatattg tttgtttcag
     5881 cgatatccag caagtactcg cgcaaacgag gaagcatgat caaacctgga tcttggatac
     5941 gcatcaatgt accttctcct aaatgtccga atgtgccatt tttcgtcact gtatcatctg
     6001 ctgcagagca gtctactgca aagaacagat ctggcttgaa tttattgaca gaagctttag
     6061 aaccacgcaa gcctacttct tcctggacat tcgcgcctgc aatcaatgta tgacctaatg
     6121 tttcattttg caacgcttct aaagcttcta ataccattgt acagccatag cggttgtccc
     6181 atgatttact gataatattc tttccgttgg ctgttttgat cgtttcagcg taaggaacga
     6241 ttgtgtctcc tggaaggaca ccgtaactca ttgcctcttc tcttgattca aacccagcat
     6301 cgaacaaaat atcggttact tctactgatt tttgtccgct tgttcctcgc aataaatgtg
     6361 ggggaacgga agaagaaata catggataat tcttaccagt agaggtttta agtgtaaaac
     6421 gctgagcaga aacaacgtaa ggattccagc cgcctagagg tacgacttta aaaagcccgt
     6481 tttcttggat ttgtgtcagc atgaatccga cttcatccat atgtgctgcg accattacac
     6541 ggggtgcatc cgcttcttca tgatggcgca gaccaaaaat gccgcccaaa ccatctaact
     6601 gtaactcatc taccagcggt tccatatgac tttccatata agcgcgaaca tcttgttcaa
     6661 aaccgcttgt tccttgtaat tctgttaact ctttgattcg ttgaaatgtt ttttcttcca
     6721 tatgctattc gtctaaattt cagactaaac tcctctcaga cttatattga tctgcggata
     6781 aaatatgatt ttttctgcag accgatcgta tttattatag cttatttttt ctggtagcgg
     6841 ttatttattt caaagaaaat atgagagtgt ggtaaaataa gcacaaggag taacaagtga
     6901 gaagaaggag gtatgtccac atgtatgaag aaagcgaact aagctatttt aaaggcgggt
     6961 tagcagttgg aatcggtcta ggattattga gcggggtagc ttcaacgatt tggtatcatc
     7021 gcaaaaaagt attgtcagca gaccaagtac ttgaaaatat caagtcatct tttctaaaag
     7081 aaggtgcaat cgaaggttct tggatctctt ttgaaaagga acctattagg aaattcgccg
     7141 ttcggtcgag tgcttatcgg ggaggtcttt ctcgaataga agatggcgaa actgtttatt
     7201 atgaattttt agcagatgct catacaggaa ccgtattaga aatttcaaga aaaaaaggtg
     7261 caacagatta atacgtcaaa aaagtgagag aacgaatacg cacccactat tcaaaagaga
     7321 ggtatcatgt ccttcttttg tagtgggtgg ttttttagat agagctttcc aaacatatat
     7381 tccagtattt tctttttaaa taaatgatat ttagcgggaa aaactagcat ttcctttgat
     7441 tatgaggtat ttttaattta gcttttgttt tctatagtta aggagagtga aaaatggaac
     7501 aacaagaaaa aattgctgtt ctgattcctt gttacaatga ggaagcaaca atttcaacag
     7561 tcattgcaga ctttaaacga gaattaccag aagcagatat ttatgtctat gataataatt
     7621 cgaccgatcg aacatacgaa ttggccgttc aaggtggagc aatcgtaaaa aaagaacctc
     7681 gtcaaggaaa aggaaatgtg atccgccaaa tgttctttga tatcgatgcc gactattatt
     7741 taatggtaga tggcgatgat acttatcctg cagaagctgt acatggtttg ctcgagaaat
     7801 tacgttctgg tgaagcagat atggtcattg gtgatcgcct atcaaatgga acctactttg
     7861 atgagaataa gagacctttc cacgattttg gaaataacct tgttagaaat acaattacta
     7921 gaatgtataa aacaaaaatt cttgatgtca tgaccggcta tcgaggattc aatcgtatct
     7981 ttgtcaaaag ttttcctatt atgagttctg gttttcaaat cgaaacggaa ttgacgatcc
     8041 atgctttaga taaaaaattc aagttcgttg aaatcccaat cgattatcgt gatcgtcctg
     8101 aaggcagcga atcaaaattg aatacattct ctgacggatt caaagtgatc atgatgatta
     8161 ttaaaatgtt taaagattat aagcctttgc ttttctttgg gatttggacc atcttcttct
     8221 ttttatttgg attggtcacg gggattcctg tgattcgcga atatatgctg acaagcttta
     8281 ttacacgcat cccttctgcg attttaagta ctggattgat gactttagca cttctttcat
     8341 tagtaacagg gttgattttg gatacagtcg tcacgaacgc taagaaagaa tatgaattga
     8401 atctatatca tgtttatcat gaataccaca gtagaaagta aaaaaaggat caagactgca
     8461 aaaataattt gcaattcttg atcctctttt taattaatta tttcgccaaa cgatagatgg
     8521 catctgcata gattgctgtt gctctgaaca agtcatcaag actcataaat tcgtttgctt
     8581 gatgcattgt atcagtatat cctgggaaca tcgctccata agcaacgcca cgtttcaaca
     8641 aacgtccgta agttccgcca ccgatgattt gttcgtagcc tttttctcca gtgtggtctt
     8701 catagacttg caataatgta gccaccaatg gatcatccat tggtacgtaa tgtggttcca
     8761 ttacgcgagc acctcgagta actgttgcgc cttcttgccc cactgtttgt tcgatgtcac
     8821 tttgcaatcc gtctactgtt acaccttttg gaaaacggaa attcatattg atgaagttcg
     8881 cttcctctgt accattttct tcaaaagcaa atagtccagc attcattgtc aatttgccca
     8941 ttttttcatc ttcataagcg actcctagtt tttctccata aaaatcttca tggacataag
     9001 cagctgctac atggataaat tgtttagcag atcctaggaa ttcatagtta tctaagaaca
     9061 atgccaagaa agaacctgca ttgataccag attgtgggct tgcaccatgt gcgcctttac
     9121 caactaattc gatttttaca tacgtattgt ccgcttcaat cgttccgctt actggatttg
     9181 cttcgatgaa ttggtaaaat gcttcttcca tcgcgatagc tgcatctgca cttggtactt
     9241 gaagtgctgc agttgctgtt cctggcacca tattttcgcg aagtccggaa acaaaagatt
     9301 tcaagacata atctcctgca ttgtcgcctt tgaagcgtaa agctaaagaa acatttcctt
     9361 tttcaccatt gatgattggg aattcagcat ctggtgagaa accaaaatct ggttcttctt
     9421 catgttcgaa ataataagcc atatcgcccc agccgctttc ttcatcgctg ccgactacga
     9481 agcgaacttt ttttgatagt ttcaagtcaa gttctttaat gatcttcatc gcatagtaag
     9541 ctgccatact tggacctttg tcatcgcttg atccgcgtgc aaagatcttt ccatccttga
     9601 ttactggttc ataaggatct gtgtcccagc catctcctgc aggaacgaca tccatgtgtc
     9661 caaatatccc tagcgtttcg tctccttcgc caagatcaat atgccctgca tagttatcga
     9721 cattttttac tttaaatcca tcacgttcgc cataagctaa catatgtttc aatgcgtcgc
     9781 gaggtccagg tccaaatggt gcatctggtg ttacttttga gtcatcgcgc tcgctgttga
     9841 cacgtaaaag atttttcaag tcttcaagca agtcttcttt acgtgcttct acttcttttt
     9901 gccaatcaat tgtcataaag ttaatcctcc acttatttaa gtcttcgtgt tcatgatacc
     9961 acaaattaca gaaaaaaata gttcaaaaaa tctgacaatt ccttttctta tctgtttttc
    10021 tctaaaaaaa atatacgcta taatgagtaa aaggaggttt ttagatgctg ataaacgaac
    10081 aactagtgaa aagagtcatc acacctcgtc caaaaatttc acataaagga acatttggac
    10141 gtatcgtttt gattggcggc aatgaacaat atggcggagc aatcattatg agtgctttag
    10201 ctgccgtaca cagcggagcg ggtctcacga ctgtagtaac tgatgaaaaa aatcggagtg
    10261 ctcttcacag tcatctgcca gaagcaatga ttgtagtttg gacggatact gaccagtttc
    10321 tttccgtaat aaagcaagca gatgtactct tgattggtcc cggattgggt acttcttcta
    10381 agagtttgga acgtttcaaa caagtactag aaaaccaatc aaaagaacaa tggctggtga
    10441 ttgatggctc tgctttaagt ttacttgctg ctaatcatct tttgctaccc tatcccgaac
    10501 aaactgtact tacacctcat caaatggagt ggcaacggtt gagttctcta ccgatacaag
    10561 aacaacggga taaagaaaac aaaaagaagc aacaggaaat cggtgctgtg ctcgttttga
    10621 aaagtcatga gacaaaaatt tatggtcacc agcacttcta caccaatcct ttaggttctg
    10681 ctgctatggc aacgggcggc atgggtgaca ctttagctgg gatgatcaca ggatttttag
    10741 cccagtttcc tcaaaatgac caaacgattg cagcagctat ttatcttcat agtttcattg
    10801 gagaccagct ggcaaaagaa caatatattg ttttgccgac acaaatcagc gaagcacttc
    10861 cgaaatggat gaattatttt tgtaaaaaat caatggaaaa cgaggcatga gtcatggaga
    10921 aatttacagg ttatgtgcga gaaccttttt attatgaaac agatcaaatg gggattattc
    10981 atcacagcaa ttatatccgc tggtttgaag aggctcgggt agccttattg gtacatctag
    11041 ggtttcccta taaaaaaatc gaagaatctg ggatcatcat ccccgttctg gaagtaaatt
    11101 gcagctataa agaaatgatc cattacggag aaaaagtgag catccatcca actatccaaa
    11161 agtatacggg gacacggttg gattttagct acgaaattgt gggaactgaa gacaaaaaat
    11221 taaaaactac tggcagtagt aaacactgtt ttttactttc tgattcgcaa cgattagtga
    11281 agctgagcaa agtcaatccg gagttagatc agttattcca agcgtacgct tctttggatg
    11341 atgtaaattc ttaataaaga ccgtctaagc atttttcttg tattgtgtcg agtagttcgt
    11401 ataatgaaaa gaaaaaagca ggtgatcccc atgtatgtcg ttaaagtaat gcacggttac
    11461 atcgataaaa ctggctgtcg cactcgggaa aaaaacttag acaatctgct cattttcaaa
    11521 gacaaaaaag aatccgaagc ttttgctaaa agaatcggcg gtcgtgtcaa accgatccaa
    11581 gaggtacgac cagattgatt caatattttt cttttcgagg ctctaatagg catgcctatc
    11641 agggcttttt ttgcagactg tgtttttatg cccacacgat ttgtaagcga tttatgttat
    11701 aatagattat atctgcatag aaaggaaggt taaatggtta tcaataattt gcaacagcaa
    11761 gcttatgaat ctatacgtaa acaaatcatt tattccgagt tagcacctgg aagtaaaatc
    11821 tccgataaag tgttagaaga acaactagct atcgggcgta caccgattcg agaagcactg
    11881 atccaactgc gcaatcaaga gcttgtaaaa accattccac agtccggcac ttatgtttca
    11941 aaaatcgata tccgatctgc ttcattagcc cgttatacgc gggaaaagtt ggaaaaaccg
    12001 atcctgcaag aatgcagtgc gaaaatgacc aagcaagatc aagctaaact agaaaaaata
    12061 ctagaacaaa ccgatcaggc agtaatcgct caaaataaaa aagctttttt tgatttggat
    12121 actgattttc accgaacttg ttatgaaatt gctggaaaac gagaaatttg ggattggcta
    12181 gaaagttata gtacgcgttt cagatggtta cgtttgacga tttcagaatt agactggggt
    12241 cgagtattag atgaacatca gacaatgctt caatcaatga ttgatcataa ctttgatgaa
    12301 gtcggatttc tttgcacgat gcatttacat atgattatcg aggagcaaga atacgtgatt
    12361 cataattacc ccgattattt tgaagatatt caggacagac ccatcaaata agaggttttg
    12421 gaatcagcgt tttgacctga gtattagaga aagaaacggc aaaatagttc ctcatatttt
    12481 gccgtttttt ttgagctaat accgagggtc agctgattgc acaccgttta ttgagaggtt
    12541 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa gtaaaaatag cttcttatgt
    12601 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt tttccaagag
    12661 gtcgtgagcc tgccgttcat ctccgagtca caaggaacaa tttcaaaaaa cagcttctca
    12721 tgtttgttga atattttgac ttgtgaccga ggagttgaca ggagaacatc gtttattcgg
    12781 aaaaaaagga ctgtgacacg acttttgtca cagtctcttt atttgtaatc taaatcatac
    12841 tccgtacatc ttttttctat tttttttatt tcttctaaga tcccttggct acgaatttca
    12901 ttatatggcc aaagtaagac tgcctctaat acataaggcg aaaaatcgct ttgattcatc
    12961 tcttttgaaa aactcatcgc tgctatctcg ctcaatgcac gaatgtttga ttgatgtgta
    13021 taaaaaggca accgccacag cacaattttt tcgctttgcg tataaattcc cttaacagaa
    13081 gcttcgatgt ggtgaaatag ttcatgtcca ataatcaaag cttgcgctgt ttctttagag
    13141 atcgaagcta gtcctgaaca ttttaatttt tctaacggct ccgtcatcaa tcgaatccct
    13201 tcatcttctg cataagttgc aaaagtgatt cgttcgttta tttgctctga ttcacaggaa
    13261 acgattggaa cagccatttg ttcacaaatt tctcctacat cggcaaaagg gtactttttt
    13321 ttgatgctac atgccgtttc gataccacaa tcgattgatt gttgtataag aattttctgc
    13381 ttggacaaag gaatcttttt ccaaaataaa tctcgccgaa actggtagaa tccccaaatt
    13441 tcttcgggga tctgttgtaa ttcgtttagt aactcttcta acttatgttt gtttgatatt
    13501 tccggcaacg acgatcactt cttcctccgg ttccatagta gatacttcca gatccatcaa
    13561 aagctgtagt tgttccagat ccgtcgctgt aaaatcagag actcttgctc ctaagcagag
    13621 ataaaattct ggtcgggcaa tcaattcagt ttgataaact gccatatctt cccaaagttg
    13681 ttcgacagct gtcatataat tggctgcagt tgttttcata catgaagcat gtccacgttg
    13741 gaccttgata aatccttttt gatcgatgat ccgaagactt ccagcatttt ttgaacgatt
    13801 ctgttcgccg catacataaa agaaaggtgt attttccaat aagcaaactt catttttatt
    13861 taatcgcata tcctctgcag caagctctag tgcttcttct gttgtgatct ccttggttag
    13921 atcggttgct ttcacttccg tagaaccagt agcgatagcc gttacttttg aagtctgtgg
    13981 atcgatttct acgtggactt cgatcgattc tggtgtagcg cctgattcaa tcgctttatt
    14041 catcgcttca tttttcaacg atcgtatatc ttctttactt ggcgaaggga tgatacgttc
    14101 tacaacatct cggaccattg caagtgctac accaatagaa gaaatcactt cggcgttttc
    14161 aggaatcgaa tatttcacac ccattttatt actaaagtaa gtaatcaatg aggcagcgcc
    14221 gccccctaca cctacaagag aaatctgatc tttctccaag tgatacttgt ctgccaattc
    14281 taaaatcacc ggttcaatct tagcatagga tttttccatg atctgttctg cgatgtcttc
    14341 aaccgtcgta tgacaataat cagctaatgc ttggatggct tttcgtgcag atgggacatt
    14401 tccataagaa aaatgttctt cttgcactaa tccaaggaca ttggctgcac atgtatttgt
    14461 aatagtcact tcttctccgt cttccatcac taccttgacg taatcagcag ggtctccttc
    14521 tttaggagag aaaaattcaa ccttaggacc tttgattttt tctgtctccg taaatactgc
    14581 ataatctaga ccggcaatat gtgcagaacg tggaccgaca tcgatgattc ctgactgatt
    14641 agcccgtacc atcgaaccgc ctgccacacc tagaacccga acatctaatg aagagatata
    14701 tgttgggtgg ccacctacaa ttgaataatc gattgctgga cgtccatttt taatgacccc
    14761 gatattggtt gttgttccgc ctacttcaaa ataaacacca ttagacgctc tcaagtacat
    14821 caaagagccc atgactgatg ctgctggacc ggaaagcatg gttaaaacag gtcgtttttt
    14881 catttcattg atttccatta ctccgccgtc tccgcgcata atcatcaaag aaacattgac
    14941 ccctgcttct cgtacggaat cttctgtaga ggttgccgta tctagcattt ttggtaaaat
    15001 agatgcattg attgctgctg tccgagttct tcttgtcaat ccatataatt ttgtgatgtc
    15061 agaagccatc gttgtaggga tcgattttac cgatgctgct tcataaacaa ctcgttctgg
    15121 ttccccatta tctactccaa atgccataga agaaaccaag acctccgctc tttctctttc
    15181 taaagaagaa atcgtttctg aaacacggtc tacatttaag tgtttgacag gtaagaatgc
    15241 attgacaatc tctatttttt tcttattccc taaatcgata tcgttcaatc tagtttgtcg
    15301 ttttgctaaa aaaccttcta atccaccttt tgccatcccg attacgccga cttttgctac
    15361 gtctccttca atcagtgcat tagtcgcttg tgtggtagaa tgtgcaacaa acacaacatc
    15421 ttctggactg atattatttt ccagcaaaca attttgaaaa gactgaacga cacctgcggc
    15481 tactccacgg acatcatcgt gggttgtttt cactgaagat tttccgatga tttcgtgcgt
    15541 cgcattatcg attgcgactg ctttcgtatg tgtaccgcca acatcaattc ccattcgcac
    15601 aaaacgtttc attttctttt cctccattca tctaatcgtt tttatcgacg ttttgtttac
    15661 taggcgttcc cgtacaataa gaacgtgatc aggcataaga tcgctccgat gatccaacca
    15721 gttgggatgg atttcttcat atattctcga gaagttactt ttgtataacc taatccccaa
    15781 gcaacccaag attgtgtaat atctaaatgc tgaggagcaa tcgttgtgat tgcaaataac
    15841 gggaacaaaa aggctggcga catcgttgga ttaacagcta atacgacagc taaaatagct
    15901 gagcctgaac cgactagatt cattggaccg cggaaaaatc ctagtggtgt caatatagca
    15961 aatacgattg ccaagactaa tggtgattgc ggtatccaat ctccaaaaat agctgaaaaa
    16021 taaggagaag catacgcagc tgaattattg aacatcgcta aggtcatcaa gaaaccaacc
    16081 attggcgcaa catctactgc gccatctgta aataatttcg caaactgacg acagatttct
    16141 ttataacctc cgcgcaattt tccagtaaac agcaatgccc agatacctgc tagaataaaa
    16201 cctaagatga ttggaagatc taaaagaacg acccctaaaa ctggtaataa tacggctaac
    16261 catgagatca ttggtgcgtc gccaccttca gctggtacgt ttgctgccat tgcatagcgt
    16321 ttctttttgt tcattgatat attagcgacc gttagtacca caactaagct tacaatcatc
    16381 ccaatcatac caatttggaa atagtcatta taagtatagc tttcagccgc tggattgaaa
    16441 ccagcataaa tcgtttgata ttgtttgaaa ttgacgatat tagcaaagat tccagccata
    16501 atcgagccca taaatgagaa cagtgcaacc ggtactgaaa tacctaatga catcaagatt
    16561 ggcaacacaa taactgcaat agaaatcact ggtccgattc ctgtcatcga catgaatata
    16621 acagcggtta caatacatag taagctcatc gtgattcttg gcttgtcccc tccaagttca
    16681 actacctttc gaatcagggt agctgcgatt ccgctatcta ctagaacacg cccgaaaaag
    16741 gcaccaaaga agacatttac caagatcgat ttggcgtact cagctggccc cgtcgtatag
    16801 atatgcgtca acacatcaat tactccttgc ccttccatcg ccgggtttgg tgaaaacgtg
    16861 tttccgacta gcgctaatcc catccataca gtagccatga tggcaaagcc gaccatcaag
    16921 ttgtagcctt ttacacagta ccagaccatg aaaaagaatg agataactaa caaaatacca
    16981 ataataatat cactcatgtt attcctccgt ccattttgtt tttctgataa gcaaactaat
    17041 atatcagtca aactcattct aacttatgta gttagcgctt tcattaaagg gtgaccataa
    17101 atgttcagtt ttcaattaaa aaaccttaat tatgctattt tagacttata cgttattgtc
    17161 ctgtttcatt tgatggagga ttgtaaataa aaaaagagga aagaacataa cttttcgtta
    17221 tcttctttcc tctttataat tgtttagctt atttagccaa agcttctttt gcttggtcag
    17281 ccaatgcagt aaatgctgct gcatcgttaa cagccaagtc agctaacatt ttacggttga
    17341 tgtcgatttc agcaactttc aaaccgtgca ttaattttga gtagctcaag ccattcatac
    17401 gagctgctgc gttgatacga gcaatccaca atttacggaa gtcgcgtttc ttttgacgac
    17461 gatcgcgata tgcgtagttg tatgaattca ttacttgttc ttttgctgtt ttaaataaag
    17521 tgtgttttga accgtagtaa ccttttgcta attttagcac tcttttacga cgtttacgag
    17581 ttactgttcc acctttaaca cgtgccatgt ataattcctc ctaataaaaa taaatcttca
    17641 gattctgtta tttgtacttc ttatttcatt tttgctagtt gttggcgaat acgtttgaag
    17701 tcgccactag aaaccatgct tgctttacgc aattgacggc gttgtttttt tgttttgccg
    17761 tggaaacggt gacttgtaaa cgcgcggaag cgtttcaatc cgcctttacc agtacgtttg
    17821 aaacgttttg ctgatccgcg gtgtgttttt tgttttggca tgactatatt tcctcctcaa
    17881 aatctttcgt tatccgacta tcggctgtcc aacagccagt tagtcattca aattacttac
    17941 tgtcgttttt cggtgccagc gtcaagaaca tgctgcgtcc gtccattttc gctttttgtt
    18001 ccactgttgc aatatccgca gtttcttcag ctaagcgatt caagactttt tgaccaattt
    18061 ctttatgggt aatggcacgg cctttgaaac ggatagaagc tttcacttta tcgccctttt
    18121 ccaagaactt acgtgcatta cgaagttttg tattgaagtc attcaagtcg atcgtaggac
    18181 ttaagcgaac ttcttttaca ttgatcactt tttgtttttt acgcgcttcg cggtctttct
    18241 tttgttgttc gaaacggtat ttaccgtagt ccatgattcg cgcaactggt ggtttcgcac
    18301 caggggcaac taacacaaga tccaagtttg cttgttctgc aatattcaat gcttctgctt
    18361 ttgtcttaac tcctaattgc tcaccatctg aaccgatcaa tcgtaactct cgtgcacgaa
    18421 tgccgtcgtt aaccatcata tcctttgcta tggtcattca cctccaaata ttatgagaga
    18481 aagaaaagct taccgaataa acggcaataa aaaaacgagt tctcttacag aacccgcgcg
    18541 tacttcacct aatagaacca gtgaaaacta tctagcccag tgacattatc aactaaggcg
    18601 agaagcgggt gcttctgctt ttatttctca accatcatag tatagcaaaa actaatcttt
    18661 ttgtcaatgt ttttcataca gtttgtcttc cttttctgaa aatgattaga ttagcagact
    18721 ttctagcagt gattcccgct tctttgctat actaaagaaa aacgaggtga acccacatga
    18781 aaaaaatcga acctattcca ccttctcttc ctcttcttac aaagggagag ttttatttag
    18841 aagatgagga gatcttctct ggtcttctga tcaacggggg tgaccaaagc tatctcgatg
    18901 ccaaaaatat ggtattaaga gagagtcata tccaaaaact tacgatgcaa aaaacagttt
    18961 tacgtcattt tgaatgcagt aatgtcattt ttgaaaaatg tgatttttcc aatctggagt
    19021 ggattggtgg cagtttccac caagtcgtgt ttcatcaatg taaattaaca ggtacgaatt
    19081 ttgccgaaag ttatcttcga gactgtactt tcactgattg tattgcttcg atggcttcct
    19141 ttagtagtac aaacttgaaa gctgtctcct tcgatcattg tcaattagaa gacagcgaat
    19201 tttatgaggt cacttggaaa aacctttttt tgtctgacaa tcagttgacc ggttcgaact
    19261 ggtttcgaac ttctctgaag ggattagact ttacgactaa tttcttcaca gcaatcgctt
    19321 tatctcaaga ttctttggcg ggactaatcg tcaaccaaga gcaagcactt gtgatcgccc
    19381 aagcccttgg tttgcagatc aaagactaga tcaacgaatc cgccgccaca gtgcaataag
    19441 catccaaata gataagacca aactgatgat caaggcgatt ccccatccga aaacggaatg
    19501 ggtaaagggt aactggagat tcattccgaa gaatcctgtc acaatagttg gaacggtcaa
    19561 gatcagcgac cagaccgtca aaaatttcat cgtgtcattc aagttgttat tcagcaaatt
    19621 attataagtg ccagagagct ggtctaaaat ctgcgaagct aagttcgtca tttcaactgc
    19681 ttgtcgtgct tcgatcagcg catcgtccaa ctcttctttt tccttttctg acaacttgcg
    19741 ataaatagcc aatgccttga tttgttctaa aagtactaca ttttgttttg ttcctgtaac
    19801 taagtaaacc aaaccgatct ctaaatctga aagttgcagc agattcttgt tcgttgtttt
    19861 ttctcttaat ttcagattaa gccgctgacg ttcactattc acttcctcga ttaaaggaaa
    19921 aaaggaatca gaaatcaaaa acaatgtacg aaacaataaa ctgtatacac tcatgtctgg
    19981 cattcgttcc aaaagcgatc gaattagtcg aatcacgtac tcggtattcg gcgtggtaaa
    20041 tgtaaacaaa ccgtcttctt ttaaaataaa agcgatagga ctagtttcat aatgattatc
    20101 gactttttgc tggtgcggaa catttgatac aagcagtaac gcttcatcaa aagaatcgta
    20161 ctctacgcgc gcccgttcat ttttatctag agaataagtc agcatctcgt cactaatgcc
    20221 atattcttct tttaagtttt taacagctag cacatcgttt tgctgtacat tgatccaaaa
    20281 attacggtct tgattaaaat agtgtttttc ccgcatactc tatcctcctg acatttgttt
    20341 ccagtataaa gaaaaaagtc gctcaaataa aggtttaccc tttatttgag caactgtaaa
    20401 tcaaatggct actttggttt ggcgaatctc tgctaatttt tcttcgatca ttttcaagac
    20461 ttcttctttt gcttgaggat cttccacaaa attatagcgg tctccatcga tttggatttt
    20521 tggactttct ttgtattctt cataccattg atcgtaacgt ctgttcaatt ctttgtaata
    20581 atcgtataga cttggatcaa aggacaattg ttcatacgaa cgaccacgtt tttcgatacg
    20641 ctccaacatc gtatcaaaag atacacgaat atgaactaat aaatccggat gttttttatg
    20701 ggcagcatag ggaagttctt ccatcatgtt ttccagcagt tcatcataca cacgaacttc
    20761 tgtttctgtt gctcttccta gatctgcatt tagatggaaa agcagtgaat cttcataaat
    20821 cgaacggtcc aatacattat tgtcatcttt cattgcttgt ttgatgcttg caaaacgttt
    20881 attcaaaaaa taaatttgca gcaaaaaagc atactgttca gggtttgcat aaaataaggg
    20941 caagacttca ttatcatcaa tcgattcata aaatgcctga ctatcaaagt gttcagccat
    21001 catttctgtt aaacttgatt ttccggcacc aattgtacct gctaaaacga tcacgctcat
    21061 ttacctccag ctcacttcat cactaaatat tgtgtttaac tccaaccttt ttaaatatac
    21121 ccccaatatc tagtagtgtc aattgtattt ttttctagtc aaagaagaaa aaaagtcaaa
    21181 aaattcttaa gaaaaaagtg ttttttcttt agaaaacgta ttcttagttt tacttctgag
    21241 atggttttag taaaataaag gaggaatgtt ttttgagacc cttagtggac aaaaaaattc
    21301 gtgaagcagc gcaactcgtt ttgggcgctt tattttttgc ttaaaaatca cttttacgtg
    21361 agaaggagtt agattatggc aaactatggc caaggagaat caagcggaaa gatcatattg
    21421 atgggcgagc acgcggttgt ttatggagaa ccagcgattg cctttccttt ctatgcaaca
    21481 aaagtcaccg cattccttga agagctggat gcaatggacg atcaactggt ttcttcctac
    21541 tattcaggaa atttagccga agctcctcat gcattaaaaa atatcaaaaa attattcatt
    21601 cacttaaaaa aacagcatga catccaaaaa aacttgcaac tgaccattga aagcacgatt
    21661 cctgctgaac gtggaatggg atcaagcgct gcagtcgcca cagcagtcac tcgtgctttt
    21721 tatgattact tagcatttcc tttgtctcgt gaaatactat tagaaaatgt ccagctttcg
    21781 gaaaaaatcg cccatggtaa tcctagtgga atcgatgcag ccgctactag cagcttgcag
    21841 ccgatttatt ttacaaaagg gcatcctttc gactactttt ctttgaacat cgatgctttt
    21901 ttgattgtcg ctgatacagg aatcaaagga caaacaagag aagccgtcaa agatgtcgct
    21961 cacctctttg aaacccagcc tcatgaaact ggacaaatga tccaaaaatt aggatacttg
    22021 acgaagcaag caaaacaagc aatcattgaa aattcaccag aaacgttggc acagacaatg
    22081 gatgaatcac aatcacttct ggaaaagctg acaatcagca atgattttct taatctattg
    22141 atccaaacag caaaagatac cggggcattg ggtgccaaat taactggcgg tggacgcggt
    22201 ggctgtatga ttgcattagc acaaacaaaa acaaaagccc aagaaatcag tcaagcactt
    22261 gaagatgcgg gtgctgctga aacttggata caaggattag gagtacatac ctatgtttaa
    22321 aggcaaagca cgcgcatata ccaacattgc tctaatcaaa tattggggta agaaaaatga
    22381 agaactcatc cttccaatga acaacagcct ttcgttaaca ttggatgcat tttatacaga
    22441 gacagaagtc atcttttctg atagctatat ggtagatgaa ttttatctag atggcacctt
    22501 gcaagacgaa aaagcaacaa aaaaagtcag tcagtttctt gacctttttc gtaaagaagc
    22561 cggcctctca ctaaaagctt cagtaatcag tcagaatttc gttcctaccg cagctggatt
    22621 agcctcctct gccagcgggc tagctgcttt agcaggcgct tgcaatactg ctcttaagct
    22681 tggattagat gatctctctc tttcaagatt tgctcgacgc gggtctggtt cagcttgccg
    22741 aagtattttc ggtggtttcg tcgaatggga aaaaggccat gacgacttaa gttcttacgc
    22801 taagccagtc ccttccgatt ctttcgaaga cgatttagca atggttttcg ttttgatcaa
    22861 cgaccagaaa aaagaagtgt ccagcagaaa tgggatgcgt cggacagtcg aaacatccag
    22921 tttttatcaa ggctggttag attccgttga aggggatcta tatcaattga aacaagcaat
    22981 caaaacaaaa gatttccaac ttctcggaga aacgatggaa agaaacggac taaaaatgca
    23041 tggaacgact ttagctgccc agcctccatt cacttactgg tctcctgaca gcttaaaagc
    23101 gatggatgcg gtgcgacaat tgagaaagca aggcattcca tgctacttta cgatggatgc
    23161 tggccctaat gtcaaagttt tagtggaaaa cagccattta tctgaggtac aagaaacatt
    23221 taccaaatta tttagcaaag aacaagtaat tactgcacat gctggtccag gaattgcgat
    23281 tattgaataa agggagattt ttatgattga agtatctgca ccaggcaaac tctatattgc
    23341 cggagaatat gcagttgttg aaacaggcca tccagcagtt atcgctgcag tcgatcaatt
    23401 cgtgacagtc actgtagaat ctgcacgaaa agtcggaagt atccaatctg cacaatatag
    23461 tgggatgcct gtacgttgga caagacgcaa cggagaatta gttttagata ttcgggaaaa
    23521 tccttttcat tatatccttg ctgctattcg cttgactgaa aagtatgcac aagaaaaaaa
    23581 cattctttta tccttttatg atctgaaagt aacaagtgag ttagacagtt caaatggccg
    23641 gaaatatgga ttgggttcta gcggtgccgt gaccgttgca acggtcaaag ccttgaatgt
    23701 tttttacgcg ttgaatttat ctcagttgga gattttcaag attgcagcac tagccaattt
    23761 agcagttcaa gataatggtt cctgcggcga catcgctgct agctgttatg gtggctggat
    23821 cgctttctca accttcgacc atccttggct ccaagaacaa gaaactcagc attctatcag
    23881 tgagttactt gccctggatt ggccaggtct atccattgag ccattgattg ctcctgaaga
    23941 tttacgttta ttgattggtt ggacgggtag ccctgcctct acttctgatt tggtcgatca
    24001 agttcaccgt tcgagagaag ataaaatggt ggcttatcag cttttcttaa aaaatagtac
    24061 agaatgtgtc aacgaaatga tcaaagggtt taaagaaaat aatgtaacgt tgatccaaca
    24121 gatgattcga aaaaaccgac aattactgca tgatttatct gcaatcactg gggtcgtcat
    24181 cgaaacgcct gctttgaaca aattgtgtaa tttagctgaa cagtatgaag gagccgcaaa
    24241 atcttctggt gcaggtgggg gcgattgcgg aatcgtaatt gttgaccaga aatctggcat
    24301 tcttccttta atgagtgcat gggaaaaagc agaaatcact ccactgccgt tacatgtcta
    24361 tagcgatcaa agaaaggaaa accgatgaat cgaaaagacg aacatgtttc actagctaaa
    24421 gcctttcatg ataaacaaaa aaacgaattt gacttcgtac ggatcatcca taaccctttg
    24481 ccgcaaatag cagtttctga tgtcgatcta agtacacagg cagttgggtt tacactaagc
    24541 agcccatttt atatcaatgc gatgactggc ggaagcgaaa aaacaaaaaa aatcaaccaa
    24601 gatcttgcaa tcgtcgcaag agaagcagat ttgatgattg ccactggttc agtcagcgct
    24661 gccttgaaag tcccttcttt agccgatact tatacgatta tgcgacaaga atatcctcac
    24721 gggaaaatca ttgccaacat cggtgcaggg acttctgtcg aaagagccaa agaagctatc
    24781 cgattgtttc acgcagatgc cttacagatt catttgaatg cacctcaaga gttggttatg
    24841 cctgagggag accgtgattt taccaattgg aaagtactta ttcaagaaac tcaaacagcc
    24901 attgacgtcc ccctcatcgt aaaagaagtc ggttttggca tgacaagaga aacgctcaat
    24961 gatctagctg ctttaggagt tcatacagtt gatatcagcg gtcgaagcgg aacaagcttc
    25021 acccagatcg aaaacgcccg tcgctccaag cgggaactga gttatttagc tgactggggg
    25081 caatcgacag tctcttcatt gcttgaagca aatgaagcag acacttctat ggaaatccta
    25141 gcctccggag gtatccgcaa tgcctatgac atatttaaag ccctttgtct cggcgcaaat
    25201 gccgtgggta cttcaggaac cgtactgacg catctgatga atcacggcgt agaagaaact
    25261 attatactga tgaagcaatg gcaagaagag cttcgtctgc tttatactat ggtcggcgca
    25321 acgaataccg cggccctaca ccaacaatct ctgatctttt ccggaccagt gaaggattgg
    25381 tgcgaagcaa gagggatcga cttagtgaag tatggaagac gcacagaaaa gagcgtggga
    25441 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt acaaacggcg gaagtaaaag
    25501 caactccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattc
    25561 cttcttattt cttggagtta aatgcttctg tcccagcctc ttttttcgaa taaatgatgt
    25621 ttaatcagct gtccttcggt attcgttcaa tccgctgatt tcacaacctc tccactaaac
    25681 agtgtactaa agctctttta aggttgtttt ccgatatagg caagtatgcc tccgtccaca
    25741 tacaagacat ggccgttaac gaaatcagat gcgctagaag ccaagaagac agatggtcca
    25801 gccaaatcga ttggctcgcc ccagcgtgct gctggcgtac gtccaacgat aaattgatcg
    25861 aatggatgac gttcaccgtt ttcttgtgtt tcgcgtaatg gagcagtttg cggtgtagca
    25921 atataacctg gtccaattcc attacattgg atattgtatt gaccgtattc agaagcgatg
    25981 tttttcgtca gcatttttaa tccgcctttt gccgcagcat aagcactgac cgtttcgcgt
    26041 cctaattcac tcatcatgga gcagatattg atgattttac cgccgccttt ttcgatcata
    26101 tccggaataa ctgcttttga aacgatgaat ggggcattta aatccacgtc gattacttgg
    26161 cggaagtctg ccgcatccat ttcgatcatc ggtgtccgtt tgatgatccc tgcattgttg
    26221 actagaatat caactgaacc tacatcttct ttgatctgtt gaaccatcgc ttgaaccgct
    26281 tcttcatcgg tcacatcgca gacataacct tttgcatcga tgcctgcttc tttataatgc
    26341 gctatacctt catcaactga ttcttgattc aagttattaa atacgatttt tgctcctgct
    26401 gtagctaaag agcgtgcaat ctcaaatcca ataccataga cagcaccggt gactaatgct
    26461 acttttccat ctaaacgaaa catatccata ctaaattcca tcattttctc tcccttatgc
    26521 ttattttaat tgatccattt gcaccatatc catgtctgta taggtgatat tttctccgca
    26581 cattgcccag atgaatgagt aattgcttgt acctacacct gaatggatcg accagcttgg
    26641 cgaaataatg gcttgttcat tgtctacaac taaatgtttt gtttcatctg gtttgcccat
    26701 catgtggaac acacgtgtat cttcattggc ataatcaaag tagacataag cttccatacg
    26761 acgttcatgt gtatgacaag gcatcgtatt ccaagcgctt cctggttcta aaatcgtata
    26821 acccatttgt aattgacagc tttcgcatac atttggatgg atgtattggt agattttacg
    26881 ttcattcagt gtcaggcctt caccagtctc cattggtttt acttggtcga tactgatttt
    26941 tacatttgga tatttatgat gagctggaac agaactaata tagaatttag ctgggttttc
    27001 agcatcttga gaagaaaata caacatgtcg tgtttctttt ccgatatagt atccgtcttg
    27061 ttttttcatt tcttcttttt gcccatcgat ctcgatgaat cctggtccac cgatattgat
    27121 cacacctagt tcacgacgct ctaagaagta atcaacacct agttctttgt ctaatgtgat
    27181 ttctaattct ttttcagtag gtgtcactcc accaaagatc attcgatcgt tgtgcgtata
    27241 agtcaaactg attttccctg gttcaaatac cttttctact aaaaattctt ttctcaattg
    27301 ttccgttgaa taatgacgga tatcttctgg actgtgtgta taacgtgttt ccatttcttg
    27361 cattttcttc tactccttct atcgaatgat ttttccagag ccgcatgcta aaaattgtgc
    27421 gacttcttct tgactaaatt ggttgcagtc cccatggatc gtatgcttca aggcagaagc
    27481 tgccgtagca aaatcaatga tttcttgcgg agacatattg gataaaatcc catggagcac
    27541 gccacctgca aaagcatctc cgccacctac tcgatccacg attggggtga ttctatggac
    27601 ttgggattcc acatattcat ggttcatcca caatgtcccc ttcaattcat tttcacttgc
    27661 tgaatgaacg gtacgcttgg tcgaataaaa cacctgaata ttcgggaact tttcctgcat
    27721 tttttgataa taataaatta gaggttgttc ttctgcttct ggcgctttct cgatacctaa
    27781 caaataaaga gcatccattt ttccagctga gcagatatct actaaaggta aaagctgatg
    27841 gattacttcg ctggcttctt gctgtgtcca taattttcca cgatagttga tatcgaagct
    27901 gatcaggcag ccagcttttt ttgcagcttc tatcacctgt ttcgtcattt tttgccattt
    27961 tttagacaaa gctggggtga ttcctgaaac atgaaatata tctattcctt ggaataactc
    28021 ctctagcgac cattccaaat gatccatttc agcaaaactt gatccggcac gatcatagat
    28081 cactgaggcg gcgcgttcgc cactaccagt ttccatataa taagtaccta gtcttgatcc
    28141 accagataat acctgtttcg tatctactcc ataacattgc aagtgctttt tgactgcttt
    28201 tcccaatgcg ttatcaggta cttttgtagc aaaaatcgct tcatgtccgt aattagctaa
    28261 agaaattgct acgtttgctt cccctccgcc ataatgggca gcgaatcgtt cacttgaatc
    28321 gatacgggtt ccctgctctg tggacaatcg caacatgatt tctccgagtg tcactacttt
    28381 tcccataagc agctacctct tgatttcttt catttttgct gcatactgtt gcgcgacttc
    28441 cgttactttg tcaaaatctc ctgtagctgc gggtgctaaa aggttcccac ctactccgac
    28501 tgttacgaca ccagcatcga accactcttt catattgtcc agactcacac cgccagttgg
    28561 catgatattg acttgaggca tcggcgcctt gaaggcagaa atgatacttg gtccataagc
    28621 gcttccagga aatagtttga cgatatctac cccgcttttc agcgctgttt tgatctcggt
    28681 aatagtcata catcctggta agtaaggaat ttgatacaga ttgcacatct ctgccgtttc
    28741 ttgatcaaag gaagggctaa caatatactc tgcgccagct aagatagcca agcgagctgt
    28801 gacagcgtct aatactgtgc ctgcgccaat cactatttct ggttggtctt tgtagaatga
    28861 caaaagttct ttgattactt gatctgcttg agggactgta aaagtcaact caataccagt
    28921 tagaccacct ttgatgattg cgtggcaagc attcaaagct tcttctttgc tttttccgcg
    28981 taccaccgca atcactccgg cgtttttcaa tcgagataaa atatcaattt ttttcatctt
    29041 ctccacctat ttcctattta gaaattttat tttataactt ggaaatctat acccatatta
    29101 tacggcagaa aagaaaaatt gcaaacgctt ttttcattat ttttgaattc tttgctcgat
    29161 ttcgcttttt gtttgcttga gggcactgat tatttgttct tttgtctcat ctgttaaacg
    29221 atatttcggc atacttacac taaatgcacc aatgatttgt tcgtttttca cgattgaggc
    29281 tcctatacaa aaaatatcac gttcgatttc ttcatcgtca aatgcgatgt gggtttcttt
    29341 taccgttttg atttcctttt tcaaccgtaa tgcattggtg atcgtatatt ctgtgtaagg
    29401 gatcaacgga tgcttttcaa gataatcagt gtactgctct tcactgaatt ctgctaatac
    29461 ggctttgccc atcgcagaat tgtataatgg tcgggtgatt ccgatttttg aagacatgcg
    29521 gatcgtttga tgttttggat ctagtttgtt cacgtacaag atctcattgt tgtccaatac
    29581 ccctaagtgg atagtctcat cgatcttttg ctgcagctgt tctaaaaacg gtaaagtcaa
    29641 ctctaccaaa tcgatttgtt caatgttttt attcgcgtag cgaataagct ttgcacctaa
    29701 acgatagttt ttctctgaat ttttatttac gtagccgatc atcaacaaag tatctagtat
    29761 tttcaagact gtagaagagg tcattcccac accctttgcg atcgtctgca aagagacatc
    29821 ttggttttcc gctaaatagt ctagtatttt agaggctttg atcaatacag ttccataagg
    29881 ctttttattt tccatataat attctcctat ttctttataa gaaaaattat agcggaaata
    29941 ccatcaattt aaaagagata tccagatata aactaccatt tctctttaca ggcaatttaa
    30001 tgttgaagat ccgcttcgtt tttacatcaa actttgatat aaagcaatga tttcttctac
    30061 tgtcgtttct cttggatttc ctggtgtgca tacatctcgc aaagcatctt ctgcaattgc
    30121 tggaatatct gcttctttta cacctaattc agaaatggtt tctgggattc caaccgcttt
    30181 gctcaaccga tcgatctcac cacaagccgc ttctcttaca tcttctaaag acatttcagc
    30241 tgctccttta atccctagaa caagagcgat ctcacggtac ttttctccag tatattcttt
    30301 gttatatttc atgacagttg gcaaaagtgc tgcacacgct acgccatgag gaatgttgta
    30361 ccatgcactt aacgggtgag ccatcccgtg taccagtcct aaaccgacat ttgagaagcc
    30421 cattcccgca atatactgtc ctaatgccat tttttctcgc ccattttgat cgccatctac
    30481 agaagcttcc aaagaatggc cgatgatttc gattgctttt aaatgcagca tatcagtcat
    30541 ttcccaagcg ccttttgtga taaacccttc aatagcatgc gtcatagcat ccatacctgt
    30601 tgatgcagcc aattttttcg gcattcccat catcatatcg ctgtcgatga acgcgacgat
    30661 tgggatatca tgaggatcaa cacaaacaaa ttttcggtga tttacttcat ctgtgatgac
    30721 gtagttgatc gttacttctg ctgctgttcc agaagtcgtt ggtatagcta atataggtaa
    30781 acatggattt ttcgtatcag caacaccttc taaactaatg acgtctgaaa actctggatt
    30841 cgtcaaaata atcccaatgg cttttgccgt atcgattggc gaacctccac cgattgcgat
    30901 caaacagtct gcctctgcct ctttaacaaa agccactcca tcttttacat tttggatcgt
    30961 tggattaggt acgatcccgt cgtataaagc ataagcgatt gctgctttat ctagaagttc
    31021 tgttactttt gttgctatac cgtgctcgat caatcctttg tcagtaatga cagctgcttt
    31081 gccgaatcct cgctgctgaa attcagggac gatcgactca atcgctcctt ttccaaaata
    31141 cgatgtttca tttaaaatca ttcgattact cattttctat tcctcctaat ttgtattatt
    31201 tttcacaata aaaacgaaag aaccccaagc aaccggttgc ccagttgctt gttgctacag
    31261 ttcaaagact ggcatcaatt cccaactgac tggagaggca tctggatttg tctccatgat
    31321 atcttccatg tacttccacc atttttgatt gatagatgta tttgccattt tttcccataa
    31381 cttttcatct tcgatcacta agtaaccaaa taacaaactt gtactcggat caagatagat
    31441 cgaatagtcg atgaccccgt gttcacgaag tgctgtacgc atttctggcc aaagttcatc
    31501 atgtcttttt ttgtattctt cttgtttttc tgggtaaact ttcatgcaaa aagctttttt
    31561 caccatgtca gacacctgct ttcgtatgaa cttcctccaa ataacctgct tttggagtga
    31621 caccaaacgc atctgctaaa tgccacaggt tttcgtccgt gattgtttgc aagattggtc
    31681 cttgcgcttt gacgtacgta tagacttctg ctgccttttc tgctgtctcg atcaaaccaa
    31741 agacttcatc catgtctttt cctgacccgt agattccatg ttgtggccat aaaaccaatc
    31801 gagtttcttt cattttttca gctgttgctt ccccaatctc atttgttccc ggaaccatcc
    31861 acggaatgat ggaaattcct tctggaaaaa caactaagca ttccgtacac atttgccata
    31921 gcgtacgact aaaggaacgt tcgtctaatt catgcgtaaa actcatcgct aatagatgac
    31981 tagcatggca atgcataacg atccgattat ctgggtctac ttctaaacgg gcgatatgac
    32041 tcatgaaatg acttggcaac tcgcttgtag gaaggcattc gttttccagc ccccacaaaa
    32101 gttccagtgt tttgccatct tcagcaactc gaacgattcc aagattctcg gcaggttcgc
    32161 tcgctacatt tttaaaatat cttcctgaac cagttacaat aaaatagcat cctgctaaac
    32221 ggctggcatc aaaaatcatc gagatgcggc ggatcacttt ttcaggatcc aagaacggtg
    32281 tgatctcttc ttcttttagt aaataagaaa tattcccgcc atttctttca tcccatccca
    32341 agcgataaag attcgacgtc gtttcgatca tttctttcac ataaggcgct tgtaaaatat
    32401 ctattttttt caatatagcc acactcctat tttcttaatt ccaacacgtc tttttgatat
    32461 tcctggactt cttccagcca atccaaacca actggaactt gatttttgta acagaaataa
    32521 ttccatacat cggcatacgg gaaatctttc aattcttccg tataagccat tcttgaagta
    32581 aagtcaaaag ccagctccat ttctttcaat cgctcaactg gttccagcat agctttcagc
    32641 agtgcttttt gtgtatttct cgtaccgatg acccaagcag cgatacggtt gatcgttgca
    32701 tcaaagaaat ccagaccgat atttgttttt tccaacaatt cgttacggac taattcttta
    32761 gcgatttcct gcaattcatc atccatcaaa accacatggt ctgaatccca acgcactgga
    32821 cgggaaacat gcaacatgat tcctttgctg aataaagcta atgaagataa tttattggaa
    32881 atcacttctg ttggatgaaa atgtccagca tctagacaaa caagtttgtc acgagtcaac
    32941 ccgtaaccca tataaaattc atgggaaccg actgtataag cttcagcgcc taaaccaaat
    33001 agtttgcttt ctactgcatc ttgtacatac cgttcatcta actcttctgt aaaaatttca
    33061 tcgagtgatt ccattaaacg ttcccgtggg gtaaaacgat caatcggatt atctttcatc
    33121 ccatcaggta cccaaaagtt attgatactt gtttgcccag tttctttccc aaagtactcc
    33181 gcgattttgc ggcttctttt cccgtgttcg atccagtatt cacgaacttc tttatctgga
    33241 tgagccaatg taaagccgtc tttcatcatt ggatgcgaaa agaaagtcgg attgaagtca
    33301 agaccgattt cttctttttt tgcccactct acccattttt caaaatgttt cggctcaatt
    33361 tcatttagat cgacagattc ttctgtatcc aaatagatag catgaaggtt caatttgtgt
    33421 ttgcccggta tcaacgaata agccttctcc agatcttgtc taagctcttc aggtgttcgt
    33481 gcggctcctg gaaaattccc tgtcgccatg attccaccgg tcaattcgcc atccggtgtc
    33541 aagaaacctt tgacatcatc tccttgccag caatgcatag aaattttgat gtcctgtatc
    33601 ttttctagta cagcatctgt atctacaccg atagttgcat atttttcttt tgcttgttcg
    33661 taatttcttt caatcgtgtt catacgatta ctcctttgat tttattcacg taccattcca
    33721 tcgtgttttg cttattttca tctgggtaaa aagtttcaca tggaaacgac tgtttgatta
    33781 catttcttgc ttgttctata ttcgaaaact gacctgtact gagcatttgc atggcaacat
    33841 tcccgattgc tgtggcttca ctaggtcctg cttctacaat gattccggca taatctgctg
    33901 tcaattgatt cagtagacga ttattcgatc cgccccctac gatgtgcagc ttttccaatt
    33961 tcgttccagt taagtgttct actttgatca gctcttgtgc gtaacaaaga gcaagattat
    34021 catacacaca acgagcgata gctcccgatg agacagggac tggctgctct gtttcaaaac
    34081 agtattgctg aatctcttct atcatatttg ggggattcaa aaatcgaggg tcattgattt
    34141 ccatcacttg ctgcagcggt ttttcttttg cagcaagctc tgccaattcc gcataactgt
    34201 actcgtactc ttgcattcgg gcaacttcct ggatcaacca catccccatg atgtttttca
    34261 aaaaacgcac cgtgttatgg gcaccccatt cattcgtata attttcagaa aaagcattgc
    34321 gagaaacatt ggcgatcttt gtttcaatcc ctaaaagcga ccatgtccca ctgctgatat
    34381 aaccccaacg ttctccttga cctggcgttc ctaatacagc tgacgccgta tcatgacttg
    34441 ctactgtgat aaaggtcaca tctggcaaat catattttga aaattgttct ttttgcagat
    34501 ctcctagtat cgttcccggc tctgtcaatg gtggaaataa ttccttcgag acaccgattt
    34561 tttcaagaag ttctatttcc cattcttttg tccctgcatt cagcagctgc atagtagatg
    34621 cattagtttt ctccgtcacc atgttaccag taaagacata ccctaaataa tcaggtatca
    34681 gtaacaattt atgtgctttt gccaaatttt ctttttcttc agcaaacaat tggaacaagg
    34741 tattgaaagg ctggatctga atccctgtct gctcatacaa cttatctaga gaatagattt
    34801 cagaaaactg ttctactgct ccattggtac gcttgtcccg ataagcaatc ggatcattta
    34861 tccgatcccc ttgttcatca atcagacagt aatcgactgc ccatgtatcg atccccgcaa
    34921 aaaggcgatc aatccctgtc tgctttaatt tttcaaatcc aatcaaaatc tctttgataa
    34981 gataatcgat cttccagcga tcgtgaccgt ctttgttccg aaagccgttt ttgaaccggt
    35041 gtacttcttc caagatcaaa gaaccttttg catccctcgt acttttcatt agtcgtccgc
    35101 ttgatgcacc gatatcgatg gcaacaaatg ttggattcat ttctccacct cttttacttt
    35161 attgttgatt agcactttat taataaagcg ttatcattta ttgacaatgc tatcataata
    35221 cagaatctta tattttgctt cacactattt tggtatataa ttgcattatc ttgacaagtt
    35281 gggagtgttt acatggatta ctgggaaaaa gccaaccact catggtcgga agactcaaaa
    35341 agagtgatct tgacgcctac acaaagaagc aggaatttat ttttttatat ccaagaaatc
    35401 ggttacttca aagcaccaat cccttactat actgaacgag cacatttacc ttcttacttg
    35461 ataaagttta ctttgtctgg caaaggttcg cttacatata atgataatca gcaacagcta
    35521 aaagctggag acttattttt tattgactgc caagaatatc agcgttatca aacgatcagc
    35581 gaagaaccat gggaaatgga ttggatccat ttgaatggtc agaacgtcca atcattttat
    35641 caggagttca caaaagacgg ccataacacc ttccattcaa caggagatcc atcagaaaac
    35701 aagatccacc atttgatcac agaattgatc cacatgcaac aaggcacgaa tgcccgtacc
    35761 gatttccagt cctctgtagc tatccacgag ttgctgaatg aactgatttt gcaaaagtat
    35821 cagctagatt tcgaagaaga ggatattccc gcctacgttt acaaactgaa gcaatactta
    35881 gacgaacatt tcaaagaaac gattacactt gatcaattag aacaacaatt cttattgaat
    35941 aaatatcagc tgagcaaaga tttttccaaa tttatcggtt ctccaccaat cgattatttg
    36001 atcagcaaaa aactttctta cgctaaagac cttcttcggt atacagaatt tacggttcga
    36061 atgatttccg aagaaatagg gattgagaat tttgcttact ttagccgcct gttcaaagca
    36121 aaaacaggac tatcgccgag agactttcgt aaattcagct gattcatata atatgtacgt
    36181 cattttttca aaatattttg aaatatatag ataaaaataa aataaccgca gtttctgctt
    36241 ctatgtaaac aacagcagct actgcggtta ttttttagtg atcattctca ttgttcatct
    36301 tcaaaatgtt cataatgaac caactgtttc tgtgtatcac gtttcaaaaa ttctttaggc
    36361 gttatgccga actctctctt gaatgctttc agaaaatgcg gataagaaga aaaaccactt
    36421 ttactatata cagcactaga gctgcggtat tctcgcaaca agtgcttggc atagatcagc
    36481 tttttcttca ttacatactg atgaaacgta aatccagtgt actttttaaa ttctcgtgtg
    36541 atatggtatt tgctgacaaa aaactgcttt tccatttcct ctaaagaaag cggatgagat
    36601 aaattttttg agatatattg taacatttgt tccacacgct cattaggaac tacagcattc
    36661 tgttcttgcg cactctcttc tttttgtaca acttgattga gaaaaatcaa gtattctacc
    36721 agagcttgtt catattttat gtcttcaccg taaatttttt tatcaggagg atggtcgata
    36781 aatgataatt tttgcttcaa tacttcctgt tctatttgca atacacgact cctcttctct
    36841 cccattggtt gaaaacaagc cgatagatta gaccatggcg tggaacgact ttctagaaaa
    36901 gaaggggtga caaagatata gacacgctcg aatacatctg aagattggcg gacgatacga
    36961 tgcaaatcat tggaatggat gagcagcaca cttcctgctt tgatcgtaaa ttgtcgtccg
    37021 ttcaaataaa aagtcgcttc tccctttaat gtagcatgga tctcatagaa atcatgatga
    37081 tgatagatcg ttttattttg gctgacgaca tctttgacat gatagatttc atactgatca
    37141 gaaaccatcg tattgacttg ttcaccgctg attttctttt ggagcatgtg ccgcacctcc
    37201 ttttgttcac ttatgcacaa ttttattata ccacgttttt tgtaatcaaa aagagctgct
    37261 tttatatact cttataaaaa agaagagacg tggcatcttc tagccccgcc ccctccattc
    37321 taaatattag ttaaccaacg tcttctttac attattcaag aagacttctt ccataacgaa
    37381 tcccatcatc ttttgtttct tcaagaacca catacaggta cggacgattc ttcatgatga
    37441 tttctttgat ttttgtgtac tcgatcacac cgtctcccaa atttaccggc acgatttttt
    37501 cctgttcgac acgaaaatct ttcaaatgaa ccgcacatat cttttcacca aaacgttcaa
    37561 aagcttcttc taccaattgg acttgttctt gataatcact acttgtgatc aaattggtcg
    37621 gatctaaaat gattcctaga tagtccgaat ctactgcttg gatcagctgc tctacccttg
    37681 ctaaagaata caccggatga ttcaaacctg gttctatccc caccatcatt tggtattttt
    37741 ctccagcttt taccaagcga cggataacgg acacagcttc agcaaatgct tcatctgtga
    37801 aattttcttc cgtatattgg atttccggca aaacacatcc agtttccgaa gcaaccatcg
    37861 acgcaccaaa ggaagaagca tagcgaagat actgctcgaa ctttgtcagc aactcttgtc
    37921 gtatcgttaa atctggatga atcatattga tatagcaact taaaattcct acggtcactt
    37981 cttgcttttc taaaactcgt ttgacataat ttcccatccc agaattgatc tcatttgcgc
    38041 cagaaggtat ttcaggaaac gaaagaccta aggcgagctg cagtgtatga atcccttgtt
    38101 cttttgtttt ttctgcaaga tcttctatcg tttggacttc ggaaagatca tgtcctctaa
    38161 ttgtaatgtt caacataact attctcctaa ctcagcatat tgggtagcca catgacgatt
    38221 tgcgggaagt aagtaatcaa aaacaagacg actaagataa ccccataaaa aggaagaagc
    38281 ggtttcagca ttgcctctgc ttttacgcct cctacacttg caccgacata tagtcctgta
    38341 cctactggcg gcgtgatgga gcctacacac agattcatga ttgaaaacaa cccgtaatgt
    38401 acaggatcaa cccctacact tgtgacgatt ggtaaaaata ttggtgtaaa aatcaagata
    38461 gcaggcgcca catccataaa catacccacc agtaataaca caagattgac taataacaga
    38521 ataataaatt tattatccgt tacgcccata atcaacgaac taattgcctg aggaataccg
    38581 gtaaacgcca tggcgaaaga catcatggaa gaagtagcca ataagaacat gatcgtccct
    38641 gacattgcta ctgattgaaa caacatatta ggtatatctt ttagttttac cgttttatag
    38701 taaaacatag caatcaacag ggagtaaatg acagctatcg ctgaagcttc tactgctgta
    38761 aatatccccg tcaagatacc accgataatg atgacaatca atagaaggct aggaatcgcc
    38821 tcttttatta tttttccagc atgagccatc tgtccttttt cgacaacagg gtatttattc
    38881 tttttagcaa cgatgaatgc tactagcatg attcctagcg cccacaaagc gcctacgaga
    38941 taacctccca taaacaaagc agagatcgaa gcaccaccag caacaagtga atacatgata
    39001 aaagcagtac ttggaggaat gaccatacct gtcggagctg acgcgatatt gaccgctgtc
    39061 gcaaacttcc ggtcatatcc ttcttccact tgctgaggga tcaacactcc gccaatcgcg
    39121 gtagaagcgg caatggccga gctagagatg gaaccaaata aagcattccc taacacattc
    39181 gtatgagcca aagcccccgg cacgcgcccg acgaacaaca tcgcaaaatc cactagtttc
    39241 ttagctatcc ctccattatt catgataatg cccgagagaa cgaaaaacgg aatagcgatc
    39301 agcgaaaaac tattgatact tcccaccatt ttttgagctg aactaaaagc agaaacatca
    39361 aacggtataa ctaacaataa agtcatcatc gatgagaatg caatacttat agcaatcggc
    39421 atccctaata caagcaacac aacaaataca aaaaataaca ctaatccggc ttctattgcc
    39481 attttattcc tccattttta tattttttct tgaattgctg gcttgttgct taatgtatcg
    39541 attagattga tcaggctata gataatgatg agtacccccg atacaggtac tgacagataa
    39601 acatatccca taggaatact taaagaaggc gagatctgcc ccattgtcaa tgtgacagca
    39661 ttccatccgc catacaccat gatcatcgca gcaaatacca gaaataaact ttgaacaaca
    39721 acttctaaaa ttttactttt tttgccagtt aatttttcac ttaaaaccgt tacggctaaa
    39781 tgtgcttttt gtcccacaac atacgcggaa gacaacatgg caaaccagac aagaccgaat
    39841 cgaacaagct cttccgtgac cgtatttgga tttcctaaaa ccgttctgga aaatacttgc
    39901 cataaaacaa cagcgaccat cccaacaagc aaaacagctc ccaggatctc taataccttg
    39961 tttaaaatca ttttcatcac agctacgctc cttgctcttg gatcttttga taaaggtctt
    40021 tgtaggtagg atcgtttttg aatatatcat gaagtggggc aactgcctct cgaaacggtg
    40081 tattatctac atcattgaat tggactccat gttcttcttg tgctaaagct ttttcttctt
    40141 caattgcttt tgcaaaagct tcaatttcaa aaaccgttga ttctttcgct gcttcttcaa
    40201 tggctttttc ctgctcatca gttagctgtt ctttgatggc atcattcatg atgatgatat
    40261 ctggcacacg tgtatgctca tcataggaat agtatttagc cacaccacca tgtccagctg
    40321 tatacaagac aaattcattg ttttctgatc cgttgatcaa attggattga agagacgtgt
    40381 acacttcatc acttcccata ggtacgggcg aaccgcctaa aagctctacc attttgatag
    40441 cggtttcgct ttgcatcaca cggatcttct ttcccttcaa atcttccggc gtatggatcg
    40501 gaccgtctac catataaaag ctgcgctgac cagaatcata ataggtcaat ccggtaaatc
    40561 ctaaattttt ggtcgattgg tagattggct ccattatttc ttcattatcc atcaccttaa
    40621 agaaatgttc ttcatcattg aatagataag gaactgaaaa aatcgaataa tctttagaga
    40681 aactttccaa cgctgaggca cttacttttg tgaaatctac tgctcctgac tgtgtcaatt
    40741 cgatcagttc acgttctcca cccagttgtc catctgggaa atactggatc tgtacggaac
    40801 catctgtttt ttcttttact aaacgcccaa attcattcat tgcatcaatt actggctggc
    40861 tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacatctgca tctgcgacag
    40921 ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg ctaagcgaac
    40981 ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta acaataagag
    41041 acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat tgctgtgatt
    41101 ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga aacgttttga
    41161 acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata agcgacttct
    41221 ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta agcaaatcct
    41281 gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg ccataaaccg
    41341 ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc gatcaataat
    41401 ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg aatagcaatc
    41461 gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt tccttcaaac
    41521 gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat atggatcata
    41581 aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc gatttttgcc
    41641 aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt ttccggtcca
    41701 tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat gacccattct
    41761 gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata caagtaagcc
    41821 atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc ttccatacga
    41881 tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat ctggtaattt
    41941 ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca gcttttgtca
    42001 tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt gatcgttgtc
    42061 aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac aaaaatctct
    42121 tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat ccttaatata
    42181 gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta aaattgacct
    42241 aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag aacttggaga
    42301 aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac gctttctttt
    42361 gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa tggttctttc
    42421 ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc tacggttgta
    42481 tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc gacagatttt
    42541 cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt ttcgcctttc
    42601 agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata gtcatattta
    42661 gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg cggtaaaaga
    42721 atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg tgcgactgag
    42781 tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga agcgttgtgc
    42841 attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata gtcaaagact
    42901 aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac tgaaacatag
    42961 gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga tgctggaact
    43021 gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata atctgctaac
    43081 ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac ttcagaacca
    43141 gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc gatcttatat
    43201 gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt gtctgggtgt
    43261 tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc aagagcgatt
    43321 actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt atttgtcgat
    43381 gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg tcgtgccaat
    43441 acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca aacgatcatg
    43501 acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc aaagaagatt
    43561 tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt tttgacattg
    43621 attaagttta cagcagagac gttacgagaa acagagttct taccataaga accgcatccc
    43681 aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc tgcagatggt
    43741 gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg caactcttcg
    43801 ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat accttcacac
    43861 aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg agaaagtttt
    43921 tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag cattttcgtt
    43981 ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg tccaacgatt
    44041 gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt ttgcaattca
    44101 tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc tgcatagatt
    44161 tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc aaatgttttc
    44221 gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat ataagctggt
    44281 acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga cttgaccatt
    44341 ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag catagaagtc
    44401 gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc ggcagcgata
    44461 gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact tggatggaaa
    44521 gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat cgttgtggaa
    44581 gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc aatttcaatc
    44641 aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt gatattatgc
    44701 caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac accgcggcct
    44761 gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc cattgccatt
    44821 tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt caatgctgca
    44881 tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt cgttttttct
    44941 ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt gttaaataat
    45001 tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt gatttttttc
    45061 acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga aaaatatata
    45121 aagttattta ttattattta tatgacacac ccgattattc aatattgagc aatatatccc
    45181 atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt ctgccctacc
    45241 tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct cttccagcgt
    45301 ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg tttcaacagt
    45361 agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg ctgcacgatc
    45421 gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat tgtcgatttc
    45481 tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt tcatgctatc
    45541 taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa aaaaccacat
    45601 tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt tcacctcact
    45661 aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta gttttttact
    45721 taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga acccgtattc
    45781 ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt gacgtacttg
    45841 tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa tcccaaatgt
    45901 ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac atgtataaca
    45961 atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt tcactactag
    46021 acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca agacacagtc
    46081 aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac cgacacccat
    46141 caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata cacgattcat
    46201 ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga aatccatccc
    46261 gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt ggatgattcc
    46321 aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg cccatcgaga
    46381 cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg gacattcgtc
    46441 aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt tttctggtga
    46501 taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt cttcgatatt
    46561 ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa tcggttgatc
    46621 ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac cagggcgtag
    46681 ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca actcttctgg
    46741 ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg tggggaaagt
    46801 tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt tcttgattaa
    46861 acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa aatatcttga
    46921 caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg cttccttcta
    46981 ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa agaaaaacat
    47041 tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa aggagggaaa
    47101 aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa aaggacgttg
    47161 gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta ctattcttct
    47221 tattatgatt cctattatct tgtttgccac caccatatat gatggatcag cgagcgtctc
    47281 agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta tttccagcat
    47341 cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt tacgtggaca
    47401 aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt tgtttatcgg
    47461 cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg gtttgctatt
    47521 agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta tttactacga
    47581 tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg caagtcgaaa
    47641 attgatggat ggttataaag gacaactttt ctggttagat ctttcattta tcggctggca
    47701 tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat acatttctgc
    47761 aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag tgtgacttgt
    47821 gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa acattcagcc
    47881 cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt atagttccag
    47941 taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt ccggtaatcg
    48001 atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc ctaccaagaa
    48061 ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg gcagcaaagc
    48121 aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt tattggtcgc
    48181 taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca gaatcgatga
    48241 ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt ctacccatgg
    48301 gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga tggtgaagac
    48361 ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt catttttcgg
    48421 aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag caaatcctgg
    48481 aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg gtgctcccca
    48541 tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc ttagcaaaaa
    48601 gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag cgtctacgat
    48661 cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca catctgttcc
    48721 tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat cattcacacc
    48781 atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga tccggatctt
    48841 gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat ctaattcttc
    48901 gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat tcaagatacc
    48961 taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca tgactgtttt
    49021 gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg gtggatcgat
    49081 cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag aaatatcttg
    49141 cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc gtttaccaaa
    49201 gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg gagcaagaac
    49261 atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa ctgttgtcat
    49321 catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt cttctaattc
    49381 tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg ttggattacc
    49441 tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata gtgcagccat
    49501 tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta cttcatcttc
    49561 tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg ttttgtctga
    49621 gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga tgatcgcatg
    49681 tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa cagtcaaggt
    49741 ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa ggagtggttc
    49801 tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga tcatactgat
    49861 tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag tattttcgtt
    49921 caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga cgatcgcttt
    49981 cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt cacctaatga
    50041 ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt ctccagtcaa
    50101 cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg cagaaatagc
    50161 atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact caacagtggt
    50221 cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca aggattccag
    50281 tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca caacgatagc
    50341 gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg tataaaagga
    50401 aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc gaaagaattt
    50461 tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct tttttcgccg
    50521 ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta ccttctttac
    50581 ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc ttcgtacaaa
    50641 aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa agggcttatc
    50701 gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg ctaccaaagg
    50761 atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag aatttctcga
    50821 tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga catgcgtgta
    50881 gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag tatccgaaac
    50941 aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt gaataggaac
    51001 aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta acatgaagaa
    51061 attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt ttatttgaat
    51121 ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt tgtcataaaa
    51181 tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt atttcacgaa
    51241 catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct ggtaagcaga
    51301 taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg attttggcaa
    51361 atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg taatgaaaat
    51421 atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa tcgtttctaa
    51481 tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac gtacttggtt
    51541 caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat ggtgttcttc
    51601 aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg tcagatagac
    51661 taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat gcaatccagc
    51721 tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc gatctgggtc
    51781 ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt caagaatctc
    51841 tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca attcttcttt
    51901 cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt tggcacgttt
    51961 cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa gaaggacttc
    52021 taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa ctcgtgtatt
    52081 attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga aatgtttatc
    52141 ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc ggtcttttaa
    52201 tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg ttgttaacat
    52261 atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg ttctgttggc
    52321 aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac taccctattt
    52381 gtattttttc attgttttag gcatactagt aatagatagt tcttcattga aaatagaaaa
    52441 agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg tggaaaagtc
    52501 gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt tatcggtacg
    52561 agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga tccaacacaa
    52621 gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga attgcgacgt
    52681 cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat ccgtgaaaat
    52741 atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa aacagctgaa
    52801 aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc acatgaatta
    52861 tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa tcaagatatc
    52921 atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc cttgcaagac
    52981 cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac ccatgacatg
    53041 gatgaagcga ttaaactagc aagccgtatc gccatcatga gtgaaggccg tgtgatccaa
    53101 tttgatacac cagaaaatat tttacgtcat cctgcaaacg atttcgtaga agagttgatt
    53161 ggagaagatc gcctactaca agccaagcca aatgcgacaa gagtcggcga agttatgcta
    53221 aatacagcaa tcacgattac accggaaaaa tcattacaag aagcaatcaa actgatgagg
    53281 gaaaaacgtg tagatacgtt gttagttacc gataattcga atgtattaaa aggatttatc
    53341 gatgtagaaa cgttgaataa aaaacgagga aaagtatcca gtgtcggcga cattttgaat
    53401 aaacatgttt tttacgtaaa agaatccgcc tatcttcgtg ataccttgca acgcatttta
    53461 aaacgcgggc tgaaatacgt gccggtcgtt gatgaacaga acaaagtcgt aggtatcctg
    53521 actcgtgcat cattagtgga tatcgtctac gatgtgattt ggggagaaga tcccgcaact
    53581 actgatgttg cagagtcagc tactcctgaa gaatctaagg aggtctgacc tatgcagcag
    53641 ttttttcatc agcacggttc agaaattctc tcaaaaggtt ttgaacatct ttatatttca
    53701 ggacttgctt tgatcattgg tatcatcatt gctgtaccgc ttggtgtctt actgacacgt
    53761 tttccaaaag ctgccacgat tgtgattggc ttaaccagtg ccttgcagac aatcccttct
    53821 cttgctttat tggcattgat gatcccgcta tttggcgtag gtaaattacc tgcgattatc
    53881 gcactcttta tttattcatt attgccgatt ttacgtaata cgtacattgg gatgaaaaat
    53941 gtcgattgga attatcggga tgtggcaaaa ggcatgggga tgactgagtt ccaatccatt
    54001 ttttccgttg aattgcccat tgctatgcca acgatcatgg ctggtattcg tttagctgcc
    54061 gtttacgtga ttgcatgggc tacacttgcc tcttatatcg gcgctggtgg tttaggcgat
    54121 ttgattttta gtggactgaa taattaccaa ccagatctca ttttggcagg tacgatccct
    54181 gtaacaattt tagcactatt ggctgattgg cttctaggtc tcttggaaca ccggctgaca
    54241 ccgatcgcac ttagagagga gaatgaagaa tgaaacgtat actaaaaatg accatggttc
    54301 ttttctctct tcttatactt gcgagttgtt ccttccctgg tcttgccagc aatacagatg
    54361 aagacaccat ttcgatcaca ggtggaatca cttcagaagc acagatctta gcaagtctag
    54421 ttgccggaat ggttgaacat tatacagata aaaataccgc aatcatcaac aatctggcta
    54481 ctacaactat caaccatcaa gcgatgatga acggagatgc ttcaatttca gcggctcgct
    54541 acacaggaac agatttaacg actacattga accttcctcc tgaaaaagat ccgaaaaaag
    54601 cgtttgctac cgtgaaagat gaatttgaga aaagatacgg tcaaacgtgg ttcccgtcct
    54661 atagttttga aaatacgtat gtctttcttg ttcgaaaaga caccgcacaa aaataccatc
    54721 tatctaaagt cagcgatctg aaaaatgtag cggatgaatt agtagctggt gtagatactt
    54781 cttggatcaa tcgtaaagga gacggttatg acggcttcca agaaacttat ggcttctcct
    54841 tcaattcgat attacctatg cagattggac ttgtttatga cgcagtcgaa gcaggaaaaa
    54901 tggatattgt tcttggctac tctacggatg gacggatcgc cagttatgat ctagtcatgt
    54961 tggaagatga tcgtcaattc ttcccaccat atgatgctgc tccagttgta gacaacaaga
    55021 tattagctga tacgcctcat ttaaaagaag ccttaaataa gttaggtcat accatcagta
    55081 cggaaaaaat gcaggaattg aactacgaag cagataataa tctagtggaa ccatcagtag
    55141 tagcacaacg tttcttacaa gaaaaccact attttgaagg gaagtgaagc aattggataa
    55201 aatgaatgtc tttcaacaat ttttctatta tttccaggaa aatggctcct atatctttgc
    55261 acaatttatc cgtcacttcc tgatttctat ctatggtgtc ctctttgcag cagttgtcgg
    55321 gattccggtt gggatcatga tttctaggag acgtaaatta gcaaactggg tgattcgttt
    55381 ggcgaatatc atccagacga tcccttctct agccatgatc tccattttaa tcattggatt
    55441 aggcctggga gtcaatgtcg tgattgttac tgtatttttg tattccctct taccgatcat
    55501 caagaatacg tataccggga tgatccaagt agacaaaaac atccttgatg tcggcaaagg
    55561 catggggatg actgctagac aacgactatt catggtagag ttaccactct ctgtttctgt
    55621 tatcatggca ggcataagaa acgcgttagt tgttgccatc gggataaccg cgatcggtgc
    55681 attcgtcggt gctggtggtc ttggagatat cattatccga ggaaccaatg caacagaggg
    55741 cacatcgatt attttagcag gtgcattgcc aactgcatta atggcgatca tcacggactg
    55801 gcttttaggt attttggaaa gacggctaga tccagccagc cgtacatcac gctgatgtta
    55861 ttgtaaaaaa caaaagcttc tgcgaccatt tgttggtcgc aaaggctttt ttagttctgt
    55921 catttttaag gcaataatcc actctgattc ttccctcctt atgggcaaag agttgctttt
    55981 cttagctatt tatccattcc gtatactcca acttcatact gccaacgggt tcatatctgc
    56041 atccaccgac taagtttttt tcttatccgc taaggcattt tgccaatcca ttttcccatc
    56101 actcgtcatg gaattcatac cacatgatct ctaaatactc gtctgcttgt tctttgaatt
    56161 gggcatcaat tcttacatcc tttggcgtgg ctttcattta agccttttcc atcagtcctc
    56221 gattgttcga tgccttcagc tgcatcatca aaactggtga ctgccgtatc ggtaacttcg
    56281 atcatctttt atacaatcgg gaacttgcca tcctcatcct tggctaaaaa catatctagc
    56341 cattccagct tctcgccatg ggaggtttgc ggttcctaaa accggtacac gattcttatg
    56401 gactgcatcc gtcgcaacta gacttggcga tacgatgatc cttcaccaga agagcacccc
    56461 cgtaaaccaa ttgatcgata tattgccaat aagaaaagac attggcgtaa aagatatcga
    56521 tccctcgtga tgcattgccg ttgatactgc tattcatggc cgaaagcgcc acgactttaa
    56581 cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc tttggatacc cgctctgcta
    56641 aaggaacgac actggtattg tatttcgtat ctgtatcttt tccaaaagac catgccaacg
    56701 aatcctctgg gaaccaataa gacgactccg gttgattgtc cattgctttt ttcaacatac
    56761 ttttggctgt ttctcgataa acgacatttc caattgtctt cgcctcactt tggcgataat
    56821 cgatggtcgt tgccactgcc aacactaaca acgatgctcc aattttactg attttccttg
    56881 tcacacttct tcctccttgt tttacataaa cccgtcaaca ttccattcct aaactcaaac
    56941 aatgaccctt caaaggtgga gatataggaa caaattaaag aaatcatgca ataaatccaa
    57001 gaaggttggt tttagaacaa aaaaagagtc tcctttttgg ggagactctt tgttaactat
    57061 ctaaaaaggt tggctcttct cacttcactc ataatgtgtc caggcagaat aaatttctac
    57121 aactttattt tcctcatcaa ctttgtaaac cactcgatgc tgtctattca gcctgcgtga
    57181 gtatctgcct tcatgtgtag gtctcagctt ttcaaaagac tgcgtgggca tatatggatc
    57241 ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt aaattcgttt gcttgatttt
    57301 tcttaaatcc actttcgcag agtttttgat tgcgactgta tagttactca tcaaagagct
    57361 tcccaatcaa tatcatcgat attcgtaaag ctactgtcgt ctttttcgcg gtctcgtact
    57421 ttatctaaag tacctgtttg ctcaagaaaa agggtttctt gaatcgctct ccagtcttcc
    57481 agcccaatca atacagcatt gtttccacta cgatcactaa taatctcgat ttcagtatga
    57541 ttttcattta cctctttcaa taactgataa aaatttttac gagcagtgct aggattcatc
    57601 gttgtattag ccattgtctc tcctcctttt tctgatataa ttatagcgta cttgattaag
    57661 tacgtcaaaa aaatacaata aagcacttaa attcagcaaa cttttcctct tttttacata
    57721 gagaaaaaga ccccttttta gggaggatct ttgtccacgc tcacttctta ctcctttacg
    57781 actagcggta gccaaacgta aagtaattgg taacaacgta tcatctttag caaaggtcga
    57841 atccaaagcc tgtgagtcta tgcaacaaca ccaatacacg aaggttcctc gttgcagtgt
    57901 gacttgattg atatcttgaa taacctgagg atacgcacga aagatttgta acggttattc
    57961 aaaactgatt ttggccgtca agtcttctac tgcctcaaaa atgattgcct ctttcgtctc
    58021 ctgtcattca tgttcactat cgatttgata cttgcttaga ccgttgggtt tgtgaatcac
    58081 caattaaaaa tggcactgag ttatggtaac aaaaaatggt cacttgacga ggctgagatt
    58141 cctacttatg aagacacatg gcacttaaga gtcgaggtaa aggaagaagc tgaaaatata
    58201 ggaggctgta cattatttgg caaaggattc ggaaattatg atcaagacat caaaatcaaa
    58261 atgttttaca gctaaatagg aagaataata acatttcata cgctgttccc atctttaatg
    58321 gcttatagag ggcaccttta gctgagaata agacaaaaca gaccctctag cttcaattaa
    58381 gagctagagg gtctgttttt gattttaaaa cttggtttat ttcttcaaat ggtagctata
    58441 ggtgctgaca acgatatttt cacgccaaga caatcgcaat cttaaccgca aacttgtttg
    58501 gttatggaga atattctgcc agcgtctgtt tggtacaaat tgaggaatca acacagtagt
    58561 tgtatagttt tgtttcgctg catttttact tacgatatct acataacgga taattggatt
    58621 ttcaatcgag cgataagaag agtggacgat tgaaaaacgg acatctggga aatgcttttt
    58681 aaattctgct tcgatttctt tttctttttc gacatcttcg tctagcgaaa catgcatcgc
    58741 aacgacataa tcaccgatcg aacgtgcgta attcaacgca ccgatattta cccgtgtcac
    58801 attcccgact aatacaagaa cggtattccc gtcataatca tgtagttttg catcattttc
    58861 taaacgaagc tgttctgcta cttttttata atgatcatgg atcttataaa aaatgaacat
    58921 aacgattggc ataatgatga agaaaggcca aatgtcccct aaccgataga caaacaaaat
    58981 cgcaatgatt gcatatgaga tgaacgcacc cgtaatattg gcaatcgatt tgcttaacca
    59041 tctttttcct tcttttttcc atttgacgac catccctgtt tgagataaag caaatggaat
    59101 gaacacgccg atagagtata acggaatcaa gcgttctgtt gagccatgga aaataaacag
    59161 caatacgatc gaacctagcg ctagtgtgat aatcccattg gaatagccta gtcgatcccc
    59221 acgatcttga tacatgtgag gcatgaattt atcttttgcc agattgtatg caagaactgg
    59281 aaaagcagaa aaaccggtgt tagcagcaac tgctaaaatc agcgctgtag caaattgaag
    59341 gacgtaatac aaaataccat gtccaaagac agctttacca atttgagaca aaacagtgac
    59401 ttctttttca ggaacgatcc cataccaata gttgataaat gtgatcccca cgaagaagaa
    59461 acctaagatc aatgccatca tcgtcaatgt agcagcggca ttttttgctc ttggcttctt
    59521 aaagaatggt acagcattac taatcgcctc caccccagtt aaagaggaag aaccagaaga
    59581 aaatgctcgt aaaatcaaag cgatagaaac acccggcaca gccgctcctg gaagtgcagt
    59641 agcttgcaat ggcacagcac ctgtcacgat attatataaa ccaacaacga tcagtaacga
    59701 gatcacaagg ataaatgtgt acaccggaaa taataggaac gatgcagatt ccctcagccc
    59761 acgaagattc agcatcatca gtagcaaaac gattgtcaca gaaatagcga cttgatgacc
    59821 gtagagtgct ggcaccgctg atgtgatcgc ttcagcacct gctgagacag atactgctac
    59881 tgtcaacatg tagtcgatca ataaagagcc accggaaatc aagccagcat tctttccaag
    59941 attctcactg ctgacgacat atgctccccc gccatgagga taagcatgga tgatttgacg
    60001 atatgatagc gttaatgaaa tcaataaaat aataacaaaa gcggcaatgg gtagcgaata
    60061 ccagatggct gcagcagaca aagcgactaa aacgacaacg atttgttccg taccatatgc
    60121 aatagatgat aacgcatcag acgacaataa tgccaaagca gcaaatcggg ttaatttgtg
    60181 ctcatcattt tcagcagatt ttaaaggctt acctactaac aaccttttta agtaatccaa
    60241 tttctaaact cctttacaca caattttact tcaagtttta tagtcgaaaa tttatagtaa
    60301 acaagatgtt atagtacgct cactgacctt aaaagtcaat aaactttaag aaaataattg
    60361 gaaaaaaaca gaaaaatatc aagcgctttt tcaaacgctt taatacagca aaaatctaac
    60421 atttttttat gggctttttc tttgaattct ataataaaag gatagaactt gacttagaaa
    60481 atcaagttct atcctttttg atatcttaac ttttggaaga gagatgtaag gctgctttct
    60541 acccatcatt cttactactc gaagcatagc gcagttgtca caacctcttg ataaacggtg
    60601 tttaaaagct tgcatctgcg gtaataagcc caaccaaatt aaaaacataa gaagctgttt
    60661 ttatttggtc gttcttatta cttgatgcag agcagctttt ttacaacctc ttggtacacg
    60721 gtgttcaatc agctgaccct cggtattagc tcaaaaaaac ggcaaaatat gaggaactat
    60781 ttttccattt ttttctctaa tactcaggtc aaaacactga tttcacaacc tcttttttta
    60841 atgaaaaata tggctctcat gcgccgcttc tttgaatttc gtcatttttt cttcgatgaa
    60901 cggatagatt gccgttccta cgattccaaa gacaacaaac aaaccgatca tgatccagat
    60961 tgctttcgta gcatttggcc aataaattcc tcctgcagat tcccgtaaca agtttaccgc
    61021 atgagtaaat ggcaagaacg gattgatcat ttggaagaat ttccctgaga cttggatcgg
    61081 atagttcccg cctccgccag agatcgacag tactaagatg ataatagcga tccctttacc
    61141 gacattatcg aataatgcag ccaagacata gacgatcatc atgaacgcta aggcgatcaa
    61201 gacagcaaat gacacgctgt agaccggatt ttggacatct actcctagta agaaaatatt
    61261 ccctaatgta acgatcaagc tttgcgcaat cgccattgtc aaaaatgtca gcatacgagc
    61321 aacgaattgt tctcgtttcg taaaattgtt tcggtctttc tttcctaaat agtattctgt
    61381 cgtagcaaca cttgaaagaa gaacagcgcc gacccacaga catagtgcag tataaaacgg
    61441 ggtgctagct gaaccgttat ttgcgattgg ataaagcgca ttggtttgta gctcaacagg
    61501 ttgtgtaaag aagtcacttt cttttgttgc gtctagtttc aataatttga tgacttcgcc
    61561 tagatcgact tcttgttcgc cttttcggat cgcgtctgct gctttttgga taccagcttt
    61621 gatatttggc cagtcattgt tgatcaagtc cgcagcagcg ttcacagctg tttcgacttc
    61681 tggcattttt tcatttactg ttgtcaatgt accggtgatt tcatttttta gtgctggcca
    61741 atcattttgg ataaaatcag cggccaatcc taatttttgc tgaatgatcg gtaattcatt
    61801 gttgtatagg tctgcccctt cattgatgcc attgacgatg gtatccatat ttccattcag
    61861 cattgtattg gcatcatgga tttcttgtcc aattgcaggc atttcttttt gatatttttc
    61921 taaaattgcc actgcattcg ttacagttgc tttcgttgag ctgagaagcg aatcaaagtc
    61981 gatctggtcc gctttgctca gtgcgccttg tgcagtccca atcgtatcga tgacttggtc
    62041 tagtagagaa gtgacagtgg aactgatttg acctgtattg ataccagcaa tgatgccatt
    62101 gatattatta acaacggcat caatttgatc tagcaattca tcaatagcct tcgtgtcttc
    62161 attttgaaca gctgtgttca aagcagatag gcggttttgc agattgctta gcaagggttt
    62221 taattgattt agctgatcaa taattggctg taatttcgta tttccagcac ttgtttgcaa
    62281 tttagtcagg aactcgacca gtttatcgat tgcattttgt tgttgagata ttccgtcaat
    62341 aaagccgttc aatacattgc tgatgttttt cttttcatca tcagtcagct taccatcttc
    62401 aatagctgtt cttacagtat ctattaacga acctgtcgtt gttgccactt gtccaattga
    62461 cttcaatgta acatctacac tgtttgtgat gcttggtatc gcatctttca gctgagaggc
    62521 acctgatttt gtttggtttg ctagatcact tgcctgattt tctaaatttt tcacatctgg
    62581 caagatatct tgtacttgtt ggatgatcgt caagccttgt tttgcttcat cgattccttg
    62641 gttcattgtc tcttggatcg aagcaaaatc gccgtcgatc tctgctagtt gcttaccggc
    62701 attttggatc tctgggattt tttcttgcaa atcaaggatg atttttgctt gatctttcaa
    62761 ctcaggcatt ttgtcattca atgcaacgac tttttcaccc atctcatcta ctttagggat
    62821 gtaatccacg aattcatttg ctttattcag tttttccttg atttcaggta gttgactttg
    62881 taattcgagg acttgttttg tgtagccgtc gatcgtgtcc aaattttcat ctgttgataa
    62941 aatcatatct ttaactttat tgatacttac taggttcgta tcgatgtcgt aacctatctc
    63001 attgaatact ttcaataaag tcgaactggc tgttttgata aattcatttg tgatttgcga
    63061 ttggatcgaa cttgctcctt tatcggtgat tttcggtgca atcgcattga ttttttcatt
    63121 taccgtatat tcgatttttg gtttcttgat atcaccacta gtaaaactca gcagatcttc
    63181 tgagaaatcc tttggcaagt aaatacctgc gtaatacttt ccggaacgga ctccgtcttc
    63241 taattgttct tttgaatcaa cgaattgcca tcctaattgt ttatttttat gaagcgattc
    63301 gatgacttgc tccccaatgg ccacttcttt accttgaaac tctgctggct tgtctgcact
    63361 gtaaacagca attggcagct ctccagtatt tccatatgga tcccataatg ctttgatgtt
    63421 aaaccacgca tatagcgaag ggatgatcat gatcgcgatg atcagaagtg ttgcaatcgg
    63481 gtttttgaaa atccgtttcc agtcaagcaa atataatcga agcgtatttt tgattgactg
    63541 catagttctc ccccttcttc ttttttcagc tgaattactt taccatgtat ttcatacttt
    63601 tttcaatcgg cttttttcaa aaaagtcgaa tttttggaca tatcacggca attgtctcat
    63661 tgtaatctct tacatattga gaaataatta aggattacag cctttacttt atcatgaaat
    63721 aaaagggaaa gataatttta tgccaaagtt tttatctatt atacgtatac gcaaaaaagc
    63781 taaaacattt tttgtctttg cccctacaaa aatgtttcag cttttttcgt tttttattcc
    63841 tgtatttctt atttttcgaa tgacaggatg attttcccag caggaggatg tccttcaact
    63901 agctgatgtg cttggatgac atgcttaagt gaagcagaca gattcattga aataaaaaca
    63961 tgaaaggctc ctttttggta ggctttgatt atgccagcaa aggcttctgc atctagatat
    64021 tcctttcttg gaacaaacga ttcataaaag ccttcctttt tttgccgcaa atctagatct
    64081 gggaggtcat ttaatgcaac atacctgccg cctggtttca tgatctggat gccggtttct
    64141 cctttgatac ttcctttcgt cgcatcgatt acaaggtctg cctgatcagc aaattgcaga
    64201 cctggatttg ttttatcata agcagcaaat gctgaagctc ctaatttttt taccttttcc
    64261 tcatttttac tggatgcact ggttaagatt cggattccct tttcatggag aagctggatc
    64321 agacttgacc cgaccgcacc tgatgctcct agtatcatta cagtatctgt cggctggatt
    64381 tccgtcaaat ggttgattag attataagcc gtaattcctg gcgtgaccat tccggctgct
    64441 tcttcccaac tcattttatc tggaattttt gctacttttg ctgatggcaa cacgacttct
    64501 tctccgtaag tcccgccgac tgcatggaca gctactcgat cacctacatg aacagttgtg
    64561 acatctgaac caatctctgt cacaatacca gcgccatcat tacctggtac ataagggaat
    64621 ttcaatgttc gtatctcttt catttctcct aaacgcaagg caacatcata aggattcaca
    64681 ctgaatgctt ttatttctac acgaacatgt ccagaaccga cttctctggg atgagcatcg
    64741 atttcttcaa aaacatcttg tgcttctccg taaccgttaa ttgcaaatct tcgcatggtc
    64801 accatctccc tttttcttta agtttacgtt tatgccttta ttttgacaaa aaaaatgtct
    64861 tttcgttttg gcgcatgatt caaaacaaaa tagttgtctt ttttcgcgta acgtgttaaa
    64921 ttatttatct aaaagcgtaa agattacgat ttacgctaat aaaaaataat tggaggaatt
    64981 tgttgatggc taaaaaatca aagattgcca aagcaaaaaa acagcaagaa acgattgaac
    65041 gctatgctgc tttacgccag gaattaaaag cgcaaaaaga ttacgaaggc ttgcgtaaac
    65101 ttcctttaga tgctcgtcca gaacgattga aaaatcgtga tttaatcgat ggacgccctc
    65161 gcggatacat gcgtaaattc gggatgtccc gtataaaatt ccgtgaatta gcacataaag
    65221 gtttgattcc aggagtaaaa aaagccagct ggtaatccaa gtctggctgt aatgtaaagg
    65281 aggagggcgc atggaccact ccttggatca tcaaaaaaga tacgttcgct tcttctagca
    65341 gaaatgctgg tactagaagc agacgtatct ttttttacgc acagctggtc cttttttata
    65401 tgtaaaggct gcatggttac ttcgctatat ttatttagta acaggagtca gtgcttcgat
    65461 acccacacta attttcaatg ctcggtcaac ttctgccata atgggtcgat ccaaatggca
    65521 gaccttttct ttcaatcttg atttatcgat cgttctgatc tgctccaaca ggataacgga
    65581 gtctctttct attcctgttc cagtagcttt gatcccaatg tgagtcggaa gcttaggttt
    65641 agccattttt gccgttatgg ccgcaacaat gatcgtcggg ctaaaatggt tacctagatt
    65701 attttgaata accaaaactg ggcgtgttcc gccttgctcc gatcccacga ctggagaaag
    65761 atcagcaaaa taaatatctc ctctttttac cataggatcc tccttattct tgatattctc
    65821 ttggtatccg ctgtcctaat ccgcaagcca cttcatagtg gatcgtacct aattgatctg
    65881 ctatatcctg catagtgatt tctttgtctg catttttgcc gataagcgtc acttttgttc
    65941 cgacaggaaa ttcttgaggc aatcggatca tcagttgatc catacataca cgcccaatcg
    66001 tttcacagta gttcccttct accaatagag aaaaaccctg catctttcgt ggccagccat
    66061 ctgcataacc gatcggtatc gttccgatcc actctgcttc aggagtgata tatgtttctc
    66121 catagccgat tccttcacct gctgggagtt ttttgacttg aatcaattca gaaaccagct
    66181 caagtgctgg ctgcaatgga taaacttccg gtagtgcgtg cccagaggga ttaagtccgt
    66241 acattgctac tccataacga atcatattgc cgattgtttc atcatgccat aaagccgttg
    66301 cactattgct gacatgtaca tatcgaggaa gttcgggaag ttttttcagc acttctataa
    66361 agcgttcttt ttgcagattc caatatgtat catctgcctg atctgctgta gaaaaatggg
    66421 taaataaacc ttcccattct aatacacgac tttctttgat gaatgcagct gcctctttta
    66481 cttcttcagg tgtacaaaat ccgatacgac ccatcccagt gtctgctttt agatgaatcg
    66541 aaagaggcgt ctctagctta cttttctcta aaacagcagc tgctgcttgc aaccattccc
    66601 gtgtacctgc agttactgat aaatcatgag caacagctac agtcagtgcg tcgtaaggaa
    66661 ccatattcaa gataaggatg ggctgagtaa atccagcttc tctcaactcg actccttcat
    66721 ctaaatttga aacacaaaag cccgaagctc cacctgcaac tgctgcctca gctgtttcaa
    66781 tagctccatg accgtatccg ttcgctttta ctactgcaaa caattctttt ccttgcggaa
    66841 gtcttgcaac ttcatttgct acattttcag ttatagcttt tttatgtatc actgctttcg
    66901 ttgggcggtg ccaagcgaca accatcgttt ttccttcttt ctagtcttcc aaaataattt
    66961 gtgcaaaagc tgtttcattt gtatgagtaa tactgaccca tacattgcct tcatgaggtg
    67021 aacgcgtcac aacaggctga ccattttcat tttttaaaat ctcgacatct tgaaaggtaa
    67081 cttttccgat cccagttccc caagctttag agaaagcctc tttgcaagcg taacgcccgc
    67141 ctaaaaattc tacttggcga tgaaaaggca aggactggaa tagtttcatt tcatcagagg
    67201 tcaagattcg tgcaatgaat tttggttttt cttcgatcag ccgagtgatc ctaggaagtt
    67261 cgactgcatc gatcccaatt ccttttatca tatgcttttc tcttttcaat aaatttagaa
    67321 cactctcatt ctaccaaatt ataaccattt tgccacaaat aagtccacaa ataacaaaaa
    67381 tgaaagtttc tcttaaaaaa gagaaacttt cattttatgt ttaatcgtta tttgaacgga
    67441 ttacaaatcc gcgtttttta tcattgtgac gtttaccgcc atctttttgg ctgttattat
    67501 ttttgctata accgcctttt gatttgtttc cacgataatt accgccgtca cgacgattac
    67561 ggttgtttcc gccaccacgt ttgccatttt tattgtatcc ttttttctgc attggcaatg
    67621 ggcgttctgg tgtgatcttc actggcactg catcagctgg atcttttgct gtcgttttta
    67681 gcaaaagtgc aactaaatct tgtgcagaat attcttctaa taatttatct gctgtttgca
    67741 ggtatttatc tagaccattt tctgcaagtt ttgtttcgat ttgttcaact gcagcaccta
    67801 attgaccttt gaatgcttct ttttcggttg gtggacgtaa agtcgtcatc cgttttttcg
    67861 tcaagttttc aatcacatgc aagtagtcca tttcatttgg tgtaacgaaa gtcacagaca
    67921 taccgccttt tccggcacgg cctgtacgtc cgatacggtg aacgtagctt tctggatctt
    67981 gagggatatc atagttgtac acatgagtga cacctgaaat atctaatcca cgtgcagcaa
    68041 cgtctgtcgc tacaaggata tctaaatgac cgctcttaaa tgaacgcaat acgctcattc
    68101 gtttttgttg agacaaatcg ccatggattc cttctgcacg gtaaccgcgt gcttctaatc
    68161 cgcgtgccaa ttcgtctaca cggcgttttg tacgtccgaa gacgattgtt aattcaggtg
    68221 tttgaacatc aaacaagcga gtcatgatat cgaatttttc gtattctttt gcgcggacat
    68281 agtattgatc gatcaagtca gctgtcattt ctttcgcttt gatcttcaca tgatgtggat
    68341 ttttcatgaa tttaacgcca atattcttga tagctggcgg catcgttgct gagaaaagta
    68401 atgtttgtct ttgatcagga acttgtgaaa tgattttttc gatatcttcc aagaagccca
    68461 tattcaacat ttcatctgct tcatccaaaa ctaatgtttg aacggttcca agtttcaatg
    68521 tgtggcggtt aatgtggtca agcatacgtc ctggtgtacc cacaacgata tgtggacgat
    68581 ctttcaagcc acggatctga cggccaatat ctgctccgcc gtaaacggct tgtacacgta
    68641 cttttttgtc gcgccctaaa cgatataatt cttcttgtgt ttggatagct aattcacgtg
    68701 ttggtgcgat aactaaacct tgtaattcgt gacgatctgg atcgattttt tctaacattg
    68761 gtaatccaaa tgcagctgtt ttccctgtac ctgtctgtgc ttgtccaata acgtcttttc
    68821 cagctaatgc tagtggaatc gtttctgctt ggataggcgt tgcttcttca aaaccagcgc
    68881 gttcgactga tttcaacaat tctggtgata attctagttc tttaaatttc aaatgaatcc
    68941 tcctatatgg ttgttaactg aggatgtagc ttttttgcta ccccgtcgat ttgttcggtc
    69001 aaagtgtatt gtttcttttt gacagtggcc acgcacgttt ttatccattc ttagcccctg
    69061 tggcaccacc gaaaaatcac tttgcgaagt ttaggcatta gaaaagccaa acgggtcgcg
    69121 ttcagcttta tcattattat gacatctggc ttcaacaagt tgtttctatg gcaaaaccag
    69181 tccaatttct cttcatcttt cggcaatagg ttattctaac atagtttttc gttttctgca
    69241 agtttacaga tttttatttc atttgctgaa gttgttctac cacttcaaat aagcccatgc
    69301 tgttgctgcc ttttagcata atactatctg ttgcttcaag attttgtttg atagcatcaa
    69361 tcaatttatt cttttctttt tcaaaataga acacaggtag ttgggggtat ttttggttca
    69421 attcttcttt tagtgcatgc atttgttccc cataaaggaa aatcatggcg aatttctcag
    69481 gatcgatatg ttcactcatt tgcgcatgca tatttaaaga ctctttccct aattccagca
    69541 tatctgctaa cacagcgatt ctgcgtcctt ttgttggcag atttttaaaa gtatctaata
    69601 ccagtcccat ggctgttgga tttgcattgt agacatcgct caaaatagct gcaccatttc
    69661 ctgccgttag ccattcggtt ctgttttgag tgatagaaac atgggcgagc ccttggcgta
    69721 tctcaggtat ggacagtcca aagaagcgcc cgatcgtata cgcaatcaaa gcattcgtta
    69781 cattataact tccaggcagc gggatcgtaa atgtttctcc ttctactaca aaatcggttt
    69841 gtgcttttcc ttttgctgtc acttccgcat gaacatcgcc atttccaata ccgaatgttg
    69901 tgactgtttg tactaatgtt gccgttaaag gtgtcagcaa cggttcatct gcgggcacaa
    69961 cgagtaatcc gtctgataca agtccgtcag tgatttccat tttggcttta gcgattcctt
    70021 ctcgcgaccc aagattttct atgtgtgctt caccgatcat tgtgattgct gcagcttttg
    70081 gttgagccat aagtgaaagt tccgtgattt cattggcgtg atccatgccc atttccaaaa
    70141 cgatcttttc tgtatcttgg ggcatgtgca aaatcgtgta aggaagacca atattattgt
    70201 tgtaatttcc ttgcgtctta tatgttttgt atttttcaga caagacagac gcggttagat
    70261 ctttcgtggt ggttttacca ttgcttcctg taattgcaac gacttctgcg cctacttttt
    70321 ccaagtaata ggttgccaat tgctgcatgg ctttcaaagg atctgctaca ggaataactg
    70381 gaaaattttt tggagcttgt tcaacaggca agctccaaaa tgaagcagat gctccgtttg
    70441 tgatcgcttg ctggataaat tcatgtccat ctcgcgtacc agcaagagga acgaataaac
    70501 ttccttcggt tatcttccgg ctgtcaaatt cgacattggt aatctttgtc agtgcagagc
    70561 attcgctgcc cacggcttgt gcaatttctt gtattgttag attcattgag atctctccta
    70621 aaaaaggttg tgaaaaaagc gtttggccta aacggcgggt tcgcatgact ctcacttcca
    70681 agtaaatttc tctattcgta tttagaagct agaatgaacg cggttacgca atcactccag
    70741 cctcttcttt atttctcttt attcattttt ttcaaaaaaa gattgacgct gatggtatcg
    70801 attcattcct aactggatca gttcttcaat caaatcaccg tatttcaagc ccatattttc
    70861 ccataagagt gggtacatac tgaactccgt aaatcctggc atagagttta attcattcag
    70921 gaataattca tttttatttg tcaaaaagaa atcgcaccgg ctcaatccgc ttccacctaa
    70981 catcgtgtaa gctaacttcg cgtactcttg cgctttttga taaacttctt ctggcacttc
    71041 ggctggaatc tgcatttcga ttttattatt gatatatttt gcttcataat catagaatgc
    71101 tacgtctttt acgacttcac caggcaaagt cgtccgaacg tcttcatttc ctaatacagc
    71161 aacttcgatt tcgcgcgctt caattccttg ttcaacgatt gctcgagaat catactgata
    71221 ggctgttgct aaagcatttt gcagctcttc tcggttttct gcttttgtaa tgccgacact
    71281 agaacccata ttcgccggtt tgacaaacat cggataaagc aaagaacctt cacattgatc
    71341 aaatactttt ttaggatttt ctttccattg attcttaagt actggtacat aaggaacttg
    71401 cggcacacca gcagcttgta aaatatactt ggtcatgatt ttatccatgg cacatgcact
    71461 ggtcaatacg cctgcgccga cataaggcat attcaatgtc tctaagaagc cttggatcgt
    71521 tccatcttcc ccgtttggcc catgtaaaac tggaaaaacg atggctcctt cttctttgat
    71581 ttcgcccgga ttgatcactt ttcctgtaaa gccttcctct gtctgtccac ttgggtccca
    71641 tgaaagatgc aagacatctt tgctggcagg tttttctgtt agtaatggac ctttgaccca
    71701 ttgtccttct tttgtaataa atacgagttg aacttggtaa taattataat aaatggcatt
    71761 taaaactgaa aatgcggaaa gaatggacac ttcatgctct gcgctgcgtc cgccatatag
    71821 taaagtaatc ttcaaaagat tgtcctcctg tattctaaat cgttcagtga tttactctca
    71881 tattttagca caaaacaaaa ataaacgaat aacttattta taaaaaaacg agccagcttc
    71941 taaaagaacg agcgtcattt attttttgtc agctagatta tttgtcccgt tttttataaa
    72001 aagttatgat aaagaaaagg agggatgatg atggcgcaaa caaatccacg cagccgcttg
    72061 gcagaaatca ttcgagtgtt catcaattac aatgtagtgc caaactttgt acagcaaaaa
    72121 aatcccgaac aggtaaaaaa agcctttgaa gaactaggcc ccaccttcat caagatagga
    72181 cagatgcttt ctgttcgaga agatttgctt tcttctgcct tcactcaaac attcaaaaca
    72241 ttacaagata gtgtacctag cgacacattt tcaacggtca aaaagacgat cgaaacagaa
    72301 ctttctcttt cattatcaga tatattcgat gatttttcaa aatcgccttt tgcctctgct
    72361 tctatggggc aggctcatcg tgccaagctg aagaacggtg acgcggtagt cgttaagatc
    72421 caacacccta acatcgccga agagatccga cttgatcttc aactatttga acgagcgatc
    72481 cctttgatca aatacattcc tgaaactagc gtagttgatc taaaaggggt actccaagaa
    72541 gtaaaacgtt ccttgatcaa tgaaatggac ttcttaaaag aaagtcaaaa tggcgaacaa
    72601 ttctatcaaa agaacaatgg ctggaaagag atccgcagcc ccaagattta tgacgctttt
    72661 tgttcgaaaa aagtgatcgt gatggaagaa atgtctggta aaaacttgaa tcatttaatg
    72721 aacgccgaaa acaaaactga aacttttatc actggaattc aaaacaagca gctgaaacaa
    72781 gaagttgcaa agcttttagt tgagaacttt atgaaacagg tgtttgatga tggttttttc
    72841 catgctgatc ctcatccagg gaatctgcta ttccatgtac taacaaaaga agaacagaca
    72901 caagctagcc gaaaaactga aacagtccat gaaaaagagt tcggttcctt tgcttttcga
    72961 gcctccactt ctgcagaaga ccctgttgcg ccatatacca tcaactacat cgattttggt
    73021 atgatggggc atctttctgc aggacttagg caaaaactca cacaagccgt tttagcgctg
    73081 tatacgaaag atgcttatcg aatcgaaaaa gcggtccttc gactttgtca gcaagaaggc
    73141 tcgtttgatg aaagtcggtt ccaccaagaa ctgacaagtt ttcttgaaca gtattatgat
    73201 tctcctattg atgaaatcaa cttgcaagaa gtattcacac atgtcgtgac gatttgtcat
    73261 caaaataatc tgcagttcga tcgtgatatc acgcttttgc tgaaagcatt tgggacatta
    73321 gaaggggtga tccgcgtcct cgatcctgaa gtttccctca tggaagtcgc ttcgcctttt
    73381 gctcaacatt attttctcac tcatctggat gtagaagata cattgaaaca aagcggatta
    73441 gacttattgg aagggatgaa ggctgcgcct aagatccctc aacagctcca ccatctatta
    73501 gaaatgtgga cttcgggaca aggaaaagtc aatttggaat tgaaaaagca agacaaatta
    73561 ttgagcagga tcgaaagtat gatcaatcgg cttgttttcg gtatgatttt agctgcattg
    73621 atcgtagggt cctccctgct tgtccaagca gcacctgtag aaaacgcaga ggtcgtttcc
    73681 ttattaggga tatttacgta tgctattgcc gcattcgtca ttattttttt ggcaatcgat
    73741 gcattgatcc aaatgtataa gaagaggaaa aagtagaaaa aggttgtgac agaaagaatc
    73801 agcttcaaga aataagaagg ggttttacat aaattgtttc tcaaattttt gtgaaaattc
    73861 ggcttatttt tcgaagaggc tacttctgaa acaccgagaa taaaaaatgt gaaaaagctg
    73921 ttctgcaccc agtaataaga acaatcaaaa agggaacatg gaacacattt atcctgtttc
    73981 cctgttccct ttttgatgta ttcgagcata aataaaatac cttcgttacc tactatcttc
    74041 tgtacttggt aaaccttagt caattgcctt ttttaacgct tgacgaaaag acatatgatc
    74101 tttctccgta aagggttttg gaccctttgt ccttatgcca gcttttctta actttccact
    74161 atgataacgt tgttctagaa gcgtgccgat attcgcttcg tcaaattctt ttccagagtg
    74221 atggaacact cgcacaccct tcggcaatac aagtgaagct aaaccataat cttgcgtgat
    74281 caaaaagtca ttcttcttaa ccataccgac gattttataa tccgcactat cagctccgcg
    74341 gtctacgtag acgatttgga catgattagc gtattctttg gtcgtataat ggtccacact
    74401 ggtcacgata accacgtcaa gaccatagga ttctcctaat tttatgacat cttcttttac
    74461 tggagaacca tctccatcaa tgaatagcct cattcttttt cgccttcttc cataaatgat
    74521 aaacagttac taatccgctc agtaaaagac caacattgat ccattttgcc aagataagca
    74581 gttgtacact ttctatcggc actgattcat ggttcagcgt gatcaataat agatggattg
    74641 gctctcccat ttcttttgct aaaacaaaca aaagcgtcat caataaaaac tggtggatca
    74701 cactcccttt attttgagta gtatacttga caacgatccg gcgatccatt ggaatagctg
    74761 tatcagtttg ataatattcc ttatttagcc agctgcctgt tactacgtgc cagaaactaa
    74821 gcagaaagac acctatcgca aaaccatttg gaatcaagag gaccaataaa gaagaactca
    74881 gtccaagcaa ttgcatagcc aatggagacc atgttaaacg tttcaaaatc catggtccga
    74941 ctatttttgc tgcaatcaag cctgctgcat aaggcaaata cataaaaatc gaaagttttt
    75001 ctaatccgta cagtatgcca acataaaaac tgctaaataa aaaaaggaaa ttccctagca
    75061 ttccatttag aaaagtaaac agatttagcc agcttggcaa cttccattcc ttcttctggt
    75121 gtcctaaaac aaacacgaac aagatgaaca gcagcaagaa accataaata gcaagatcaa
    75181 aaagcctttc atcgaataac aatcgggcat ttctcaaaag aaacaataag ccaaagaata
    75241 tcaaaaataa aacaagctct cgcgtagcta ccagatttct tttgatgtct ttgaaatcca
    75301 gttcgtaacg cggataatga ctaaccgtat ggtaggccat gacgtaaaaa agtgtataaa
    75361 tagtaaagct tgctgcaatc tgagtcggtg caggcaactc aatcgcttga tacaggagaa
    75421 atagccagat agctgcaaat aagtactttt tcccatccat attgataaaa ccttgcgtct
    75481 tttcgtgaaa attgaccgtg gtattagcag gtacaagcca tgctgcactc agccctagca
    75541 aaacacttga aaaataaaat aaaggaaaat aaaatacgcc taatattcca agcaatgaac
    75601 cagcaccacc aatcagcaga gcgatcatca gcaacgtgta actatctaga ccaaatcgta
    75661 gactgttgac taaaaatagt ccagtcatcc gaaaggcata aaacagcgta aatggcaaaa
    75721 tcgtcagcca attatgcgag tctgttagtc ccaaaaataa aagataagga ataaaaataa
    75781 ccgtgttact taagataaag ggcgttgttt gtgtaaaatt acgcccaaat cgtttgagtt
    75841 tttcgaacat gacaatggct ccttaaacag tcttataact aatctgctct tcgattttag
    75901 cattaaaggg gaaataaacc aattttacag aatatattct tgtaaaaaaa aatacagaaa
    75961 gaagcataag cttctttctg catttttacg gtacttgatt actcaagcag acaagccctt
    76021 ctccacaaaa gtcttgcccg acacccctgc atccggcagg tgataaagat agaaatcaat
    76081 tatcctgtgc ctccaaaatc aattaggagc tctgtaaacc agagtccttc tcgacccgtc
    76141 gcataaattt actttaacac agttcctatg tttatgcgag agcttttttc attttataat
    76201 cgaccatttg aaatccttga ccgatatctg aaataagttc ttctgtaatc tcaaatcctt
    76261 catgcttata aacctcgatt gcttgatgat tcccctgatt cactcgcaaa aagatctctc
    76321 tttcctttgt ttgcgcagca agttcttcat accagcgaaa aactgaagaa gtcaatcctt
    76381 gaccacgtac tttatttagc aaatataatt tacttaaata aatttttcca tttatctctt
    76441 cataagcagt gtaaccgaca gtcttttctt cgctttttaa taaaaagtag gacacacctt
    76501 ctgtcagttc tttttgaatc tgtttttttg actgataagt tttcagcatg taatccactt
    76561 gtttttgacc aatgatcggc acaaaaactt ccggccatac ttcttgtatg actgccatca
    76621 gctcatccag ttcattttct gttactttta ttttttccat acacccacgt ccttttttac
    76681 attataaatg accttgcgct tgatggaata aaaagagcat gatcatgcta tacacaccca
    76741 ccgaccacgg tttcaagaaa atcacaatct gcataaattc cctaaaagat aatttcgtca
    76801 aaccagctac tagacagatc aagtcgtctg gcgcaaaggg aagcagcaag gtaacaacta
    76861 gtactttttt aatatctttc gtttttcttg tcagcaattc atcaaatttc tgatactttt
    76921 ttggcgataa aataagctgg acaaaggcta cgccatagta acggacaagc aaaaatccga
    76981 ttgcttcccc aatcactagg ccaatacaac tatataaaag cccaaatcca ttgccaaaca
    77041 tcagcatgcc cgctactgag gagatccctc ccggcaaaat aggaatgatc acttggatca
    77101 cttgcaacag aacaaatatc atcactgcat acttgccaaa ttgctggata aacgcttgca
    77161 aagacgtgct cgatcgaaaa atcccatgat gataagcata aagaatcaac acaaagaaaa
    77221 aaaacagacc aataatgggc atgatctgaa gacatttttg taaaactgat ccttttttag
    77281 acatctatat tccccacatc ttttttcatt tattaaaact gcctcct
//
LOCUS       DAZAID010000002        68586 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000002 DAZAID010000000
VERSION     DAZAID010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.002"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..874)
                     /locus_tag="ACG9BE_000076"
     CDS             complement(50..874)
                     /locus_tag="ACG9BE_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFN3030866.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(847..1527)
                     /locus_tag="ACG9BE_000077"
     CDS             complement(847..1527)
                     /locus_tag="ACG9BE_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN3030867.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1550..2488)
                     /locus_tag="ACG9BE_000078"
     CDS             complement(1550..2488)
                     /locus_tag="ACG9BE_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFN3030868.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2733..3584)
                     /gene="ispE"
                     /locus_tag="ACG9BE_000079"
     CDS             complement(2733..3584)
                     /gene="ispE"
                     /locus_tag="ACG9BE_000079"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFN3030869.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3738..6311)
                     /gene="mprF"
                     /locus_tag="ACG9BE_000080"
     CDS             complement(3738..6311)
                     /gene="mprF"
                     /locus_tag="ACG9BE_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFN3030870.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6452..7258
                     /gene="proC"
                     /locus_tag="ACG9BE_000081"
     CDS             6452..7258
                     /gene="proC"
                     /locus_tag="ACG9BE_000081"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFN3030871.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7337..8893)
                     /gene="rny"
                     /locus_tag="ACG9BE_000082"
     CDS             complement(7337..8893)
                     /gene="rny"
                     /locus_tag="ACG9BE_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFN3030872.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9304..10353)
                     /gene="recA"
                     /locus_tag="ACG9BE_000083"
     CDS             complement(9304..10353)
                     /gene="recA"
                     /locus_tag="ACG9BE_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFN3030873.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10462..11703)
                     /locus_tag="ACG9BE_000084"
     CDS             complement(10462..11703)
                     /locus_tag="ACG9BE_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFN3030874.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11943..12521)
                     /gene="pgsA"
                     /locus_tag="ACG9BE_000085"
     CDS             complement(11943..12521)
                     /gene="pgsA"
                     /locus_tag="ACG9BE_000085"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFN3030875.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12730..13653)
                     /locus_tag="ACG9BE_000086"
     CDS             complement(12730..13653)
                     /locus_tag="ACG9BE_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFN3030876.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14392..15678)
                     /locus_tag="ACG9BE_000087"
     CDS             complement(14392..15678)
                     /locus_tag="ACG9BE_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFN3030877.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15671..16939)
                     /locus_tag="ACG9BE_000088"
     CDS             complement(15671..16939)
                     /locus_tag="ACG9BE_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFN3030878.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17191..17652
                     /gene="mscL"
                     /locus_tag="ACG9BE_000089"
     CDS             17191..17652
                     /gene="mscL"
                     /locus_tag="ACG9BE_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFN3030879.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17711..18157)
                     /locus_tag="ACG9BE_000090"
     CDS             complement(17711..18157)
                     /locus_tag="ACG9BE_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3030880.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18250..18495)
                     /locus_tag="ACG9BE_000091"
     CDS             complement(18250..18495)
                     /locus_tag="ACG9BE_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFN3030881.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18610..19188)
                     /locus_tag="ACG9BE_000092"
     CDS             complement(18610..19188)
                     /locus_tag="ACG9BE_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN3030882.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(19265..19795)
                     /locus_tag="ACG9BE_000093"
     CDS             complement(19265..19795)
                     /locus_tag="ACG9BE_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFN3030883.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(19799..20659)
                     /gene="rlmB"
                     /locus_tag="ACG9BE_000094"
     CDS             complement(19799..20659)
                     /gene="rlmB"
                     /locus_tag="ACG9BE_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFN3030884.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(20652..21047)
                     /locus_tag="ACG9BE_000095"
     CDS             complement(20652..21047)
                     /locus_tag="ACG9BE_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFN3030885.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(21044..22456)
                     /gene="cysS"
                     /locus_tag="ACG9BE_000096"
     CDS             complement(21044..22456)
                     /gene="cysS"
                     /locus_tag="ACG9BE_000096"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFN3030886.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(22453..22989)
                     /gene="epsC"
                     /locus_tag="ACG9BE_000097"
     CDS             complement(22453..22989)
                     /gene="epsC"
                     /locus_tag="ACG9BE_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFN3030887.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(23075..23314)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23311..24771)
                     /gene="gltX"
                     /locus_tag="ACG9BE_000098"
     CDS             complement(23311..24771)
                     /gene="gltX"
                     /locus_tag="ACG9BE_000098"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFN3030888.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(24941..26098)
                     /locus_tag="ACG9BE_000099"
     CDS             complement(24941..26098)
                     /locus_tag="ACG9BE_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFN3030889.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(26132..27502)
                     /gene="radA"
                     /locus_tag="ACG9BE_000100"
     CDS             complement(26132..27502)
                     /gene="radA"
                     /locus_tag="ACG9BE_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFN3030890.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(27565..28092)
                     /locus_tag="ACG9BE_000101"
     CDS             complement(27565..28092)
                     /locus_tag="ACG9BE_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFN3030891.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            28322..29128
                     /gene="proB"
                     /locus_tag="ACG9BE_000102"
     CDS             28322..29128
                     /gene="proB"
                     /locus_tag="ACG9BE_000102"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFN3030892.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            29125..30372
                     /locus_tag="ACG9BE_000103"
     CDS             29125..30372
                     /locus_tag="ACG9BE_000103"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFN3030893.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            30448..30792
                     /locus_tag="ACG9BE_000104"
     CDS             30448..30792
                     /locus_tag="ACG9BE_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3030894.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(30909..31235)
                     /locus_tag="ACG9BE_000105"
     CDS             complement(30909..31235)
                     /locus_tag="ACG9BE_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3030895.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(31850..33052)
                     /locus_tag="ACG9BE_000106"
     CDS             complement(31850..33052)
                     /locus_tag="ACG9BE_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3030896.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            33169..33684
                     /locus_tag="ACG9BE_000107"
     CDS             33169..33684
                     /locus_tag="ACG9BE_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN3030897.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(33883..34329)
                     /locus_tag="ACG9BE_000108"
     CDS             complement(33883..34329)
                     /locus_tag="ACG9BE_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFN3030898.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(34376..35854)
                     /gene="msr(C)"
                     /locus_tag="ACG9BE_000109"
     CDS             complement(34376..35854)
                     /gene="msr(C)"
                     /locus_tag="ACG9BE_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFN3030899.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(36208..36837)
                     /locus_tag="ACG9BE_000110"
     CDS             complement(36208..36837)
                     /locus_tag="ACG9BE_000110"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN3030900.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(36821..37285)
                     /locus_tag="ACG9BE_000111"
     CDS             complement(36821..37285)
                     /locus_tag="ACG9BE_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN3030901.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(37772..39040)
                     /locus_tag="ACG9BE_000112"
     CDS             complement(37772..39040)
                     /locus_tag="ACG9BE_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN3030902.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(39271..40008)
                     /locus_tag="ACG9BE_000113"
     CDS             complement(39271..40008)
                     /locus_tag="ACG9BE_000113"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN3030903.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(40075..40644)
                     /locus_tag="ACG9BE_000114"
     CDS             complement(40075..40644)
                     /locus_tag="ACG9BE_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFN3030904.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            40772..41644
                     /locus_tag="ACG9BE_000115"
     CDS             40772..41644
                     /locus_tag="ACG9BE_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN3030905.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            41986..42858
                     /locus_tag="ACG9BE_000116"
     CDS             41986..42858
                     /locus_tag="ACG9BE_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN3030906.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            42855..44813
                     /gene="nagE"
                     /locus_tag="ACG9BE_000117"
     CDS             42855..44813
                     /gene="nagE"
                     /locus_tag="ACG9BE_000117"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFN3030907.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(45145..45996)
                     /locus_tag="ACG9BE_000118"
     CDS             complement(45145..45996)
                     /locus_tag="ACG9BE_000118"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN3030908.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            46119..46586
                     /locus_tag="ACG9BE_000119"
     CDS             46119..46586
                     /locus_tag="ACG9BE_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFN3030909.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(46849..47331)
                     /locus_tag="ACG9BE_000120"
     CDS             complement(46849..47331)
                     /locus_tag="ACG9BE_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN3030910.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(47431..48294)
                     /locus_tag="ACG9BE_000121"
     CDS             complement(47431..48294)
                     /locus_tag="ACG9BE_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN3030911.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            48892..49767
                     /locus_tag="ACG9BE_000122"
     CDS             48892..49767
                     /locus_tag="ACG9BE_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFN3030912.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(49912..50064)
                     /locus_tag="ACG9BE_000123"
                     /pseudo
     CDS             complement(49912..50064)
                     /locus_tag="ACG9BE_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(50332..51663)
                     /locus_tag="ACG9BE_000124"
     CDS             complement(50332..51663)
                     /locus_tag="ACG9BE_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN3030913.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(51812..52525)
                     /locus_tag="ACG9BE_000125"
     CDS             complement(51812..52525)
                     /locus_tag="ACG9BE_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3030914.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            52700..54124
                     /locus_tag="ACG9BE_000126"
     CDS             52700..54124
                     /locus_tag="ACG9BE_000126"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3030915.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            54423..55130
                     /locus_tag="ACG9BE_000127"
     CDS             54423..55130
                     /locus_tag="ACG9BE_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFN3030916.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            55117..55440
                     /locus_tag="ACG9BE_000128"
     CDS             55117..55440
                     /locus_tag="ACG9BE_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFN3030917.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(55529..56566)
                     /locus_tag="ACG9BE_000129"
     CDS             complement(55529..56566)
                     /locus_tag="ACG9BE_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN3030918.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(56887..57870)
                     /locus_tag="ACG9BE_000130"
     CDS             complement(56887..57870)
                     /locus_tag="ACG9BE_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFN3030919.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(58285..59055)
                     /locus_tag="ACG9BE_000131"
     CDS             complement(58285..59055)
                     /locus_tag="ACG9BE_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3030920.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(59048..59983)
                     /locus_tag="ACG9BE_000132"
     CDS             complement(59048..59983)
                     /locus_tag="ACG9BE_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3030921.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(60299..61948)
                     /locus_tag="ACG9BE_000133"
     CDS             complement(60299..61948)
                     /locus_tag="ACG9BE_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFN3030922.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(62065..64668)
                     /locus_tag="ACG9BE_000134"
     CDS             complement(62065..64668)
                     /locus_tag="ACG9BE_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFN3030923.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(64680..65729)
                     /locus_tag="ACG9BE_000135"
     CDS             complement(64680..65729)
                     /locus_tag="ACG9BE_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN3030924.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(65755..66069)
                     /locus_tag="ACG9BE_000136"
     CDS             complement(65755..66069)
                     /locus_tag="ACG9BE_000136"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFN3030925.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(66085..66531)
                     /locus_tag="ACG9BE_000137"
     CDS             complement(66085..66531)
                     /locus_tag="ACG9BE_000137"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFN3030926.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(66532..68454)
                     /locus_tag="ACG9BE_000138"
     CDS             complement(66532..68454)
                     /locus_tag="ACG9BE_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN3030927.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT        19993 a        14398 c        11924 g        22271 t
ORIGIN      
        1 aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt tagttatttt
       61 ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac aaagcaataa
      121 agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta gttagtcctg
      181 acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg aagcttttgc
      241 ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg
      301 caatcgggat catcacagca atagccacac cagtaatcac attgaacatc atcgacatca
      361 aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga acatacatcg
      421 gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct aaaaagatga
      481 cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata cttgttgctg
      541 aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca gccattaaaa
      601 tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc aatatcacag
      661 ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta
      721 acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat tgtcttctga
      781 tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg gcaagaaacg
      841 ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg
      901 gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca ttagaactga
      961 tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc tggattcttc
     1021 atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag caaaaatacg
     1081 cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc gatttcgcat
     1141 atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt tttcagagag
     1201 acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga tcaccgtact
     1261 aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac ttaatttttt
     1321 tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca gtaatcctaa
     1381 cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa tgacaaattc
     1441 acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat agtaaaaggt
     1501 caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact taattgatac
     1561 tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg cctgctttga
     1621 tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact cctgtttcat
     1681 tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc actttgatgt
     1741 tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt tcctgatctg
     1801 gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa taagcaaaag
     1861 cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg gagtattttt
     1921 gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa gtttcctttt
     1981 tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt atagctaata
     2041 ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct tcttctgatt
     2101 cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct gcttcgatca
     2161 tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat tccatgtctg
     2221 aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt gcacttggtt
     2281 cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct tcatccccta
     2341 cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt
     2401 ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca cctatcatta
     2461 ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac aaatcggaat
     2521 tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc aatagaaatc
     2581 catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg atgagttgct
     2641 gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt aggattcctc
     2701 agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac taaataaact
     2761 tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata atgttgacac
     2821 aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc gtacttgatc
     2881 atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc ttctaaactg
     2941 tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc agcaagtccg
     3001 tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt acgtgtagag
     3061 acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg taatacttgg
     3121 atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta aggaacatct
     3181 gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag attccataat
     3241 tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa tccagctgct
     3301 actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta tttatttttt
     3361 agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa tgctttattt
     3421 gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc tgccaagtct
     3481 acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata taatacatcc
     3541 agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc acccattcga
     3601 aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct tatgtctgcc
     3661 tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt cataatccaa
     3721 acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact cggaacattt
     3781 cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa attacttcct
     3841 ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt cttttcataa
     3901 gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat gactttttct
     3961 tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc tagattggct
     4021 tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata gtcagctaat
     4081 tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc
     4141 ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc gacaccgcta
     4201 gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg tgcaccttga
     4261 agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag ttgatcgtac
     4321 caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg actcagccga
     4381 gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa
     4441 cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc aagctgatag
     4501 cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat tttaggattg
     4561 ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc aaaaacgact
     4621 tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg aagctgatgg
     4681 cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt ttcgtactca
     4741 ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa agctagcaat
     4801 gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa caaaataaaa
     4861 tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa
     4921 acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca agaataaacg
     4981 aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat caagacatat
     5041 accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc aaaagatagt
     5101 aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct agcaaggccg
     5161 agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa tgagaacggc
     5221 aataatactt gaaatagccg actgattgca gacagattag tgattgttga tagaagtacc
     5281 atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac cttttggaaa
     5341 aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg tgtgatgaac
     5401 aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata taatagccaa
     5461 actactgcta catcttgccg aacagacaat tgactcattc ctaagatcat caaaacgtca
     5521 aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc aatcatgaac
     5581 ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat gatcaaaaat
     5641 accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag caagcctttt
     5701 ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa aatgactggt
     5761 gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact ttctgactga
     5821 ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt cagcataaat
     5881 agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt tccataaaaa
     5941 ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt gatcgtgttt
     6001 gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc ttgtttttct
     6061 aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag cagtccagcc
     6121 agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca aatatcttgc
     6181 caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa cacggagccg
     6241 aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat caagtatcgt
     6301 ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc ccaatgttat
     6361 cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga tagaaaaaga
     6421 aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg gcacagggaa
     6481 tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga aagaagaggt
     6541 gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca
     6601 tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt tagccgtgaa
     6661 accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga aagtcttatt
     6721 catttctatt gcttcaggaa aatcattaat agatttagaa acagcgcttg aagatcctca
     6781 ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc
     6841 gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga tgatttttga
     6901 agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca ttggccttgc
     6961 tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga ctggtgtact
     7021 gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta tgggttcttg
     7081 tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag tttcttctcc
     7141 tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca tcccagcaat
     7201 catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata aaaaataatg
     7261 atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg ccgcggctct
     7321 tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga ataacgatta
     7381 ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata tcacgtacta
     7441 aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga acttcgcgtc
     7501 cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata ttttccagac
     7561 ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt cttgctgctg
     7621 agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc acatctccat
     7681 gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca gctaattccg
     7741 caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca atatcatgga
     7801 gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct aagataccag
     7861 ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta gttctgaatc
     7921 gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg acttcaaacg
     7981 ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat ttctcgacca
     8041 tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc aaggtcatcc
     8101 gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat
     8161 cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga ccttctcgtc
     8221 caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca gtcgtttctg
     8281 aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc gctttgcgat
     8341 ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac tcatgattca
     8401 gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa
     8461 ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt tctcgctctt
     8521 caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt
     8581 tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt
     8641 gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa cggtatttct
     8701 gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct tctttttttg
     8761 cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg
     8821 actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata gcgaggagaa
     8881 tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca tctatacagc
     8941 ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa
     9001 acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa taaacaaaga
     9061 aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac tagtttcata
     9121 taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag atgaacagat
     9181 atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt tattcaataa
     9241 aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg tttatcataa
     9301 tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg ggacagcgac
     9361 ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca tcatttcagg
     9421 atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat cttctttata
     9481 agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt ctagaagttc
     9541 tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt tgaaaggagg
     9601 tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat ctgtaccttg
     9661 tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg
     9721 tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac ggatttggtt
     9781 gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac gcaatgcttg
     9841 agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt cgatttctgc
     9901 tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc cacttgatac
     9961 taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc
    10021 atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct cagcatcaat
    10081 gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg caactgttgt
    10141 tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt aaccgccgac
    10201 acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt gttggtcgat
    10261 tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt ttttcagtgc
    10321 agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta tatttacaaa
    10381 tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag caagaaaaaa
    10441 ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa gatcaaatcc
    10501 ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat atttttccga
    10561 aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt gaccttctaa
    10621 cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg ctatctcacg
    10681 tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt ctgctaatag
    10741 ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc
    10801 tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt
    10861 caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca ctacgacttt
    10921 tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt cttggatttt
    10981 ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt tcactgtcag
    11041 tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga tctgtgtctc
    11101 aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa agcgcaaaac
    11161 tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac gtacttgttc
    11221 cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg catgtgtatc
    11281 tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa ccccgccttc
    11341 gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg tttcaaaata
    11401 tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga ggtgagccgc
    11461 agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata agagtatgag
    11521 ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat tatctcctac
    11581 aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca agaaagtggc
    11641 attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta tttctgcttt
    11701 cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt tatttaaaaa
    11761 gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat aaacagtgtt
    11821 ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa gctgtttttt
    11881 gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc actaaacgat
    11941 acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac acctgaatag
    12001 atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc gatcaggctg
    12061 aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc cgtcttgact
    12121 tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa acgcaagcct
    12181 gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc ctttccttgg
    12241 tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg gtccgcaaac
    12301 ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg
    12361 atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag tgtggtgtct
    12421 cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc
    12481 atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac tcctttcttc
    12541 cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt ccaaatgaat
    12601 ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag aaggaccgcg
    12661 acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg cgtaaactaa
    12721 tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac tctgattgtt
    12781 ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat tgcttgctgc
    12841 accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct gtgtttcatt
    12901 tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta accatacacg
    12961 attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat ttactgcgat
    13021 ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac ttgattttgt
    13081 tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg aagaggactc
    13141 ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg gatcagcttg
    13201 acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa ccaatcctaa
    13261 taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct gatgcattgc
    13321 ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc ctgtcaattt
    13381 gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag cttcagcgta
    13441 ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat catcattgtc
    13501 gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat ctaaagatat
    13561 ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag ccactcgttc
    13621 atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa gacgttatgt
    13681 ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac attgttctcc
    13741 tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg ttttttgaac
    13801 ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta aacggtgttc
    13861 aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga tatttttccg
    13921 tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct taaacggtgt
    13981 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacattaa gaagctgttt
    14041 ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc ttggtacacg
    14101 gtgttccaga agtagcttct tcggcaataa gccgaaatat cacaaaaatt tgagaagcaa
    14161 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctctttgatt
    14221 acttcgtttg attttatttt aatataagtt gattaaaaga gagcatgatt tgttttattt
    14281 tctcactaat ttttccaaca caactaagaa taccataaaa gctgattttg gaggaggttc
    14341 ctactacaag tctaaaacga aatttaagac ttttcctcat aaaatactgg tttattttgg
    14401 tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac
    14461 gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa
    14521 taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg
    14581 tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc
    14641 gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc
    14701 gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc
    14761 gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg
    14821 gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt
    14881 gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact
    14941 ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg
    15001 gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt
    15061 accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa
    15121 gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg
    15181 gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc
    15241 atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa
    15301 gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt
    15361 agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt
    15421 cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg
    15481 ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc
    15541 gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa
    15601 caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc
    15661 atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga
    15721 tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt
    15781 cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg
    15841 cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca
    15901 gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa
    15961 gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg
    16021 tatccacgct actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa
    16081 gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg
    16141 gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct
    16201 cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat
    16261 aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat
    16321 caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg
    16381 acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac cctgggatct
    16441 tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt
    16501 tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca
    16561 actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta
    16621 ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg
    16681 cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa
    16741 gctctgcaag ttgcgcactt aactttgtct gatccgaata attcaaacta tttgtttcca
    16801 acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac
    16861 ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt
    16921 ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct
    16981 tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc
    17041 aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc
    17101 aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact
    17161 aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg
    17221 cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc
    17281 gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc
    17341 acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc
    17401 gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt
    17461 gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag
    17521 caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt
    17581 cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat
    17641 acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
    17701 ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt
    17761 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
    17821 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
    17881 cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc
    17941 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
    18001 tatcttgaaa aaacgtatgc caagcttctc catcaatgtc atcaacttct tgataaggac
    18061 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
    18121 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
    18181 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tctcaggctc
    18241 gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag taagtacatc
    18301 tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat caacaacgaa
    18361 tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct ttcttcctgt
    18421 ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct tgattgaagc
    18481 taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt tttaaaattt
    18541 tcaagtttac cataaaaaaa aaagaaatgc aataaaaata aataagaagc tcattaagat
    18601 ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga gttttactct
    18661 cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca tgtctacaaa
    18721 atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa actggatttc
    18781 gcaattttcg attgcttctt ttaataaaag atgctgaatt gctaaaggtt cttgcaatgt
    18841 gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg agacttcatc
    18901 tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga tttgattgct
    18961 tataacgctt ttataataac ttccaaagct aacacctttg cttttttcgt aagttctcaa
    19021 tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat aatttcttaa
    19081 ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca gggcagcaaa
    19141 tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa aataattcct
    19201 ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg attagaacga
    19261 tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt tttaactgct
    19321 cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta ttttcttggg
    19381 ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa gttcgtaatg
    19441 ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact gtcacttgtg
    19501 tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc gtttcgtctt
    19561 ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg cccggaacta
    19621 gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa tttgataaac
    19681 gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc tggacaagtt
    19741 ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt ttcatggtct
    19801 actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca acactggcat
    19861 ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt tcttttttca
    19921 atcctggact cattcctttg ccttcattac caatgactaa agcaattgat cctttggcat
    19981 tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat cctgtttctt
    20041 tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca tgttcgactg
    20101 ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt tttggtatga
    20161 tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa ttgtgtggat
    20221 cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt gttcgttcaa
    20281 tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact ccttgatgca
    20341 cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact ggcacagctt
    20401 gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct tcttgaagaa
    20461 agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc ccaaagacga
    20521 aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt ccagtttgct
    20581 ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat tgatgtttgt
    20641 ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg gatcaattct
    20701 tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc gaaccctgtt
    20761 gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt agcatttctt
    20821 cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag catttgctga
    20881 atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg cagcttattg
    20941 ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta aatcgcatct
    21001 ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct tctcttctcc
    21061 atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac aaatcacgaa
    21121 tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct tcgatcaatt
    21181 gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag atcgaaagcc
    21241 attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc ggctggtcta
    21301 gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt gctgcattaa
    21361 aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa gcgattttat
    21421 ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa ttgtcaaacg
    21481 tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca ctgtagcgga
    21541 tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga tcaacgtttt
    21601 tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt tcatcgtctt
    21661 cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc cctgttttcg
    21721 cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc ccgccaccat
    21781 ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca atatgccaac
    21841 cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt gcttgtttcc
    21901 ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta cgctgacttg
    21961 ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt tcaaatttac
    22021 gggtacggta atacacatca cctgaagatt cataagcata gcctttttca accaacactt
    22081 gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt gtagcaggtt
    22141 cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca gcaacttctg
    22201 gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca tccgtgaaat
    22261 tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag
    22321 caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt ccgcagacat
    22381 acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg gtcaatgtat
    22441 tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa cgacttttgc
    22501 tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg cacctgcacc
    22561 gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg cagagagcat
    22621 ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt
    22681 tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa tgacgattcc
    22741 cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga tctcaacacc
    22801 agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc ggtgctgata
    22861 taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat
    22921 ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa
    22981 ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca tcttcttata
    23041 aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc gcgtggttcc
    23101 acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac gttcttggct
    23161 actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga ccactcccag
    23221 aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc ttgtaatgat
    23281 cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta aatgtgctaa
    23341 tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat gcatttgtcc
    23401 tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag tttctttttg
    23461 aacagctttg atggcagctt tgacagttgg agcatctact acttccattt gttctagctg
    23521 tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt cttttgctgc
    23581 ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga tttctgcagc
    23641 atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga ttgtcttttc
    23701 atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca tgtcagtaaa
    23761 tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc
    23821 gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca attcttggtg
    23881 ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa agttgaacat
    23941 tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga ttgattcgtc
    24001 acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat gtccgaaggt
    24061 tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat tggcaatatg
    24121 gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg caacagcaaa
    24181 attataagtt ggcataccat cgcgtttttg gatgacaaag tcgccaccga tattatcgga
    24241 ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac
    24301 acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt
    24361 caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg
    24421 ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc tagcaagcag
    24481 ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag ggccgtattc
    24541 tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt tttccaactg
    24601 gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac gaataataaa
    24661 gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg catttccaat
    24721 atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca ttttcttcct
    24781 cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg cttctcaaat
    24841 atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc
    24901 aactggttga atttccttac cagttggctg aatttctttt ttattttgat tgtttagtct
    24961 ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga
    25021 tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata cgttcattca
    25081 tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag gctactcctt
    25141 gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca gggatgacga
    25201 ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt tggaattcag
    25261 agactttatt caagttatag tcattggtta caatgacacc atctaaaagc ttagccaatt
    25321 tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac atttcgacag
    25381 atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga ccacgcacac
    25441 gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca aaattaggga
    25501 tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga ccgtcaatga
    25561 tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt cgttctaaca
    25621 gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa
    25681 ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag cctaagatga
    25741 tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg taaagagggg
    25801 tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg aagagaagat
    25861 aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct gagatatgtt
    25921 tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg
    25981 tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag ggtaaaaatg
    26041 aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt ttttgcatcg
    26101 tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc aacgtttcag
    26161 ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta cctaagttgt
    26221 ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact cgttgttcaa
    26281 tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa cattccgttg
    26341 gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct aaatcaatcg
    26401 ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga ttctgaagaa
    26461 gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg aaatcaagac
    26521 ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct tggatctcta
    26581 ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt gcaccgtcta
    26641 ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca tgctcatgca
    26701 tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc
    26761 gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata tgttctaaca
    26821 ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa atggctatcc
    26881 catttgtttt agcggttttg agtagttctg cagtggtttc tcttacttgg ctgacacttc
    26941 ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg ataacataat
    27001 caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca gcgtatagat
    27061 aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt
    27121 cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga gaaacttgta
    27181 acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata gaccctggca
    27241 ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc cgaggttctt
    27301 ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc aaacttgtcc
    27361 gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc cattttccgc
    27421 aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg caaacaaatt
    27481 gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg agacattaaa
    27541 tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc cttgacgttg
    27601 agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt ggccgatacg
    27661 ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga acatgatatg
    27721 gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc cgtttgtcaa
    27781 cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa attcatcttc
    27841 tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt tttcattttc
    27901 tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc agcctgcttt
    27961 ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag cagcatgatg
    28021 tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa cttcaaatcc
    28081 tctttttctc accggttttc ctccctaaat aaactactcc tattctaaca tatccgaggc
    28141 tgtaaaagac tttctttaca aattcttcaa gaaattctgc tatcttaaag taaaccatta
    28201 attcgttctt tttccaaacg actgcaagtt gtacaaaaaa actatttttt acatgacttt
    28261 actcgtcaaa acaagtagaa tagaacgaaa ttaatgagtg gatatttgat tgaggtgtca
    28321 catgcgtaaa gaaattaaaa atgcaaaacg aattattgtc aaagtaggaa caagtacatt
    28381 gatttatccc aatggaaata tcaatcttgc ggcaatcgat cagttagctt ttgtcctttc
    28441 tgatcttaga aatcggggaa aggaagtgat cttagtctca tcaggagctg taggcgttgg
    28501 tatgcataaa ttacatttgt cagaacgtcc gactttgatt cctgaacagc aagctgcggc
    28561 ggctgttggt caagccgaac tgatgaatat ctataatcaa agattcagtg tatataatca
    28621 acagacggca cagatcctcg tgacaagaga cgtggtagat tatcctgaaa gtcgtaaaaa
    28681 tgtgaggaat acattggaac agcttttgat tatggacacg atccctatcg tgaacgaaaa
    28741 cgatactgta gcagtggatg aattagatca tctgacaaaa tttggcgata atgatcagct
    28801 gtcagccatc atggcaaaac ttgtcgcggc agaccttttg atcgtccttt ctgatatcga
    28861 cgggttttat tcagataatc cgactacaaa tcccgaggct cagatgtatc gctatatcga
    28921 taaaatcgat gaaaagattc tggcacaagc aggtggtgct ggtagcacat ttggaacagg
    28981 cggtatgcag agtaaattaa aagccgcttc taggatattt gaagcaaaca gcgcaatgat
    29041 tttagcaaat ggaaaaaatc cggcaattat ttttgatatt cttgcaggaa aagaagtagg
    29101 tactttattt aaggaggact tgaaatgact gacttgatgc aattaggaca acaggcaaaa
    29161 gatagcgcgc aacaactagc acttatgcct acaaaccgca aaaatgagct acttgttcaa
    29221 atggcacaaa ccatcaaaaa gaatcaacaa gcgatcattg aagcaaatca taaagactta
    29281 gaaaaagcag tcgaaaacaa tatcagtgaa acgatgcaag accgcttgca gctgacacct
    29341 gagcgaatca atgccatggc tacagagatc gaaaaaattg cttcgttaga cgatcctatt
    29401 ggaaaagtag atgagatgtg gacgaacacc gacggattga gaatcggcaa aaagcgcgtg
    29461 ccactaggcg tgatcggcat catttacgaa tctcgtccaa atgtcacgac agatgcagct
    29521 agtctagcat tcaaatcagg gaatgccgtt atccttcgcg gtggcaaaga agcattcttc
    29581 tcgaatcaat tattagtcca attattgcag caagtactgg tcagtgctgg cgaatctcct
    29641 tatgccatcc agtttgtcga tgatacttct catgaaactg caggacagtt gatgcgattg
    29701 acagaatact tagatgtgct gattcctcga ggcggagcaa aattaatcca acgagtcaaa
    29761 gaacaggcaa cggtacctat cattgaaaca ggtacaggaa ataatcacgt atatatcgat
    29821 aaagaagcac agttatctat ggcagtgaac atcatcgtca atgcaaaagc ttcccgtcca
    29881 tctgtctgta acgcggcaga aacattgctg atccattctg agatcgcacc ttttttcttg
    29941 ccagctattg aaaaagaact ggtagaacac ggggtctctt tacgtgcgga tgctcgtgcg
    30001 ttagaatatc tagagacggc tgctttagca gaagacacag attgggatac tgaatatcta
    30061 gattatatcc ttgctgtcaa agtggtcgat tctttggatg aagcaattgc acatatcaat
    30121 cggcataata ctaaacattc agaaacgatc gtgacagata gttatgcggc tagtcaacgt
    30181 ttcttaaatg aagtggacgc ggcggctgtc tatgtcaatg cctccactcg ttttacagac
    30241 ggatcggtat ttggttttgg tgcagagatt ggtatctcta ctcaaaagtt gcatgcccgt
    30301 ggtccaatgg ggttaaatga attaacttcc acaaaatata tcatttatgg cgatggacaa
    30361 atccgcgaat aatgtttcgt gtgaaacatt attcccgcct tttgcctttt ttctgtaaag
    30421 gttctatcca actagaaagg tgtcaatatg tttgaaattt taatgatttt ttttatttac
    30481 cttatttctc tgaatatcgc tgcttttctg ggagtcggta ttttatccct tttctttcaa
    30541 ttcaaaaaac gaagcatagg gagtcaacgg gaaaaatggt cgcagtattt tgacaaaatc
    30601 gggcaaaaag ggctggttac acgcttgcac attagttaca tggttgcatt atgcttatta
    30661 gcgacaatca attactattc ttttttcgat cattcaatcg cttatacgat tacgctactg
    30721 attgctggga tttttcactt atcttataaa tatcagctga ataaaaacca cttgaatcgg
    30781 actttccgct aaaaaatatc cgtacgataa gtagagtgcc ttgaagatcg gcaaaaaact
    30841 gccggacttt ttagaggctg tgacaaagat tttgtcacag cctctttttc catataaacg
    30901 gcgttcgcct accagctgcc tggtaaatct gtttcctctt cgttaagaag cgaaaatccg
    30961 tcaaatgttt cgccatcata ataacgaagc agctcatcca tggacaagtt catcgttttt
    31021 tctttttctt cttccgctga ttcagccagt agctgttccc gatacacctg atacatacca
    31081 gctatttttt gctgcatcgc ttttctagtt tcaccttccg cattgattat tggcaattct
    31141 ttgatatagc cacgataaat caaccgatca tctgaaaatt caatgggtaa ttcaacgagt
    31201 gtcaacccga cttcctgaaa atattcttcc agcatctatt atccccccat tctttttctt
    31261 aaatgaattt tagcttacct actgaaaatt ttccagtttc attattttga gaaaaaaagg
    31321 gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat aaatggtgtt cttctgccaa
    31381 ctcctcaatc ataagtcaca atattcaaca aacatgagaa actgtttttt gaaattgttc
    31441 cttgtgattc agagttaaac gacagtctca caacctcttg gtaaacggcg ttcaatcagc
    31501 tgaccctcgg tattagctca aaaaacggta aaatatgaga agctattttt ccgttttgtt
    31561 tctctaatac ttaggataaa acgctgattt tacaacctct gttaaacggt gttcaatcag
    31621 ctgtccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt tccgtttgtt
    31681 tctctaatac tcaggacaaa acgctgattt tacaacctcc tgataaacgg tgttcaaaag
    31741 cttgcatctg cggtaataag cccaaccaaa cgaaaaatat gagaagctat ttttgtttgg
    31801 ctgttcttat gacttgatgc aggacagctt tttcacaacc tctgatttct tattcttctt
    31861 catttgtagc aaaataatta cggtgataaa tgatacaaca tacactgata agaacaatgg
    31921 cacctgcaaa ataaaacgca tagctttcac caatatgttc accaatcaca cctgcaataa
    31981 atggtgctaa agctccaccg aaaaagcgga caaaactata gctggacgaa gcaacgtttc
    32041 gttgcagacc tggtatttcc atagcaatcg tcgtcaacag tgtattgata attccttgga
    32101 aaaatcctgc taacacaatg ctgatcggta caaggactgg catagaagtt ccagctccta
    32161 ataacagcaa acaaataatg aataagacaa atgctgctaa aattgtccga taagtcgtaa
    32221 acttccgctc aagagccggt gcaacgaaaa tcgaagcaat agccagaagc acaccccagc
    32281 caaaaaatac aaaaccgact tctaattcag aatagccaat caataaaaat ggcgcatacg
    32341 caagtaaagt aaagaatccg aaattatata acaatgcgat tagtccaact gaacgaagtt
    32401 tatgattgct taacgctttg acaccttccc aaaggcctac ttttctcttt ggtttctcag
    32461 tttcaggtaa aagtaccgct agggctataa aaccgcaaaa catcaaaaat gctaccccaa
    32521 aaaatggata acgccatgaa ccactgccca agaccccacc gactagaggg ccaacagcca
    32581 ttccgcagcc aagtgctgct tcatacatca tgattgcttt ttctgtgtta ccaactaaaa
    32641 tactgacgat cgttgctaaa gcagtagaga caaataaagc attcccgaca ccccagccgg
    32701 cacgtaaccc aatcagcatt ccgatattcc cagataatcc gccaagacca gcgaatacaa
    32761 cgatgataaa caatcctgtc atcaatgttt ttttcaaacc gatccgacta gataagaaac
    32821 ctgtaaatag catcaccaca ccagtgacta acatgtaact agtaaataac agggtcgttt
    32881 cagctggcgt agcgtttaac tgtgcagcaa tcgtttttaa gattgggtct actagaccca
    32941 ctcccataaa agcgatcata caggtagctg ctacaactag cgctgaactt ttcccctttg
    33001 tttcttcatt tgtttgttct tgtatatcta tttgattctc caatatttcc atccctttct
    33061 ctctattagc atcatacatg atttatttta catgtcaata tgcacatgta aaacgtggaa
    33121 actttactgt gatataataa agaaaataag acgagaggta ggatttttat gaatacttta
    33181 gcctatatat tattatgcgt gcttttatcc aaaccacgtt ctgggtatga attgaagcag
    33241 ctgatcacaa ttttttggga agcccatcac agtcagatct atacaacgct tgccaagtta
    33301 gaccagcagg gttatgtgac cgttttggac aaagacgagc actctcaaaa aaagatctac
    33361 cacttgactg atgaaggacg tgctcttgta gaggaatgga tcaaagagga aactcccgct
    33421 cctacgcaaa aagatgaatt tctagcaaaa atctatgctt tttcgacgct cgataaaaca
    33481 acagctactg gactgctatt cactagagaa caacagctga acaaaatcat gcaaaaaaat
    33541 caaaaaaaat tgaatgacct ctcgctagac aagaaacaag aatttggacg ttatgttgtg
    33601 atccagcgca gaattatgct ttgtcagcag gaactccaat ggtgtcagca agtaagagaa
    33661 caaatgcagc agttttttga gtaagggata aacagaacct ttctagaaat atctattgaa
    33721 gagcaaatta tcttcaataa aagagcaata cagttggaaa agaaaaagcg atccgaacga
    33781 caggccaaaa cctatcgttc ggatcactat ttttctagaa ctgcattcaa gagactatga
    33841 caaaaagcat gccaaggcct tttttcaaat aacagtgttc catcattcat tactttgaat
    33901 tgcttttagc acacttcttg aaagcgggcc aacgatgatc aactgcaacg gtaaagcggc
    33961 aatcacattc attttccaag cattcatata agcagagaat aattgatcag gtattccttg
    34021 ttccatgatc acaccgaata aagacataca tgtcaccatt cctaaaacca tcaagcatga
    34081 aatagttaag atcatttgga ccttgctttc tggatttact ggtagtttaa aggcaatttt
    34141 tttcgccagc acaccgatga tgaagacatc caaaatgaaa gccaccacga atccaggcac
    34201 tagaccaatc aacaaacttg ataaatgaag atcttgatgc agccataaat tatacgcgct
    34261 catccccaag accatcaaaa aacacatcat tgttgtaaaa attattcctt cttttttatt
    34321 tgtaggcaat ctgtaccact cctttaaatt tgtcacgaaa atcatcgtag cacatttaaa
    34381 aatttctcgt aagtactttt tttattttct gcccttttat ttcataaact tcatcagccg
    34441 tacgctcgac aaagtaagaa tcatgagaag taaacaaaat cgttcccgga tatatttgca
    34501 ttagcttctc taaagcttcg atcgttgcca tatcaataaa attagtcggt tcatctaaca
    34561 gcaacacatt acttggctta gtaaaaagca aagcaatcgt cagacgggtc gcttctcccc
    34621 cacttaatgt acataacggt ttcgtcaaag tttcctgagc aaaccctaag tgattcagca
    34681 aactacggac aagcgattct gaaaactctg tttgtctcat gacaaaacga atgatggatt
    34741 cttcagatgt catttgataa cccttttgct gatatacttg aaagcttact ttcggagaga
    34801 gaaggattcc ttctccttgt ttgcggatat gttctaataa ggttgtctta cctgaaccat
    34861 tttcaccgac aatcgcgatt tttttaccta aagaaaactg aaaatcacca tttaccagta
    34921 atgttctccc acttctttcc aattggacat tttgtcccat gatgggatag cggctgtgga
    34981 gttcaagaga ttttggtact ggaaaacgga tttgtttcat ctgctgcggc ttggttgttt
    35041 ctcctatttt ttggagtctt ctctctaatg ttttcgcatt cttttggatg gctttttgta
    35101 cactgtcttt ttgtttagag gaagacaacc gatccagtct gatttgttgc tttttctttt
    35161 tactggacac tttttccaac ttttccgctt gttttttctt ttcttggatc gatttttcca
    35221 atcgtttttt ctctttcatg atcgactctg cttctcgtag ctgagtcttt ttctccaatt
    35281 ccttttgagt gagataggca ctataattcc ccgaaaactc tcggatttct ccgtctttta
    35341 cttcccagat tttcgatgct agctcatcta gaaaatagcg atcatgacta acaaaaacca
    35401 gcgtaccata ataataacga agctcttcaa tcagtcgatc aatacttttt ctatctagat
    35461 gggttgttgg ctcgtctaga agcatccctt gttcataaga agaaagaaga cgtatcaact
    35521 cgatctttcg ttcttcccct ccgctccatt tccgctctga agtttcttcc agtcttaact
    35581 ggaaatgact ggctaattcc aaatttaggt cctttgcttc agcgagatag gttaattgag
    35641 gcaggtagtt gaaggtgatc tctgtttgga ttttcccatg atcgggaaaa agttcaccgg
    35701 ctattagttt gagcaaagtc gatttaccga caccattttc tcctatgatc cctatacgat
    35761 cttgctgata gacacgaaga gaatcaatag atagttctag ctggttgcca aagctgactt
    35821 gtagatttgt tatatttact gctaaatttt ccatatgttt tacactccta tttcatgtaa
    35881 aacaacaatt atcgatacta aaaaagagca gaaaaatctg tttgcaaaaa aactgcatca
    35941 catcttttcc tactctgaac gaaataacta tgactaaatc accaaatctg tcatagcttt
    36001 cacctattct actgtcgttc gaaaaaatgg acatactcca tccatgaaat tgttgtttat
    36061 acatgtcttt tgaatttatt tagttgttat tcaaaagttc gaaacgtaat ttcatcgatg
    36121 cagtcatttt tccgacctcc ttttttatta ggttaccaca cacgattcaa aaaaagcaat
    36181 agctactgaa tagtaactga aaacctttta ttcgaacata gtgatctttt ctggtgctaa
    36241 acgaacagac tcgtaacctt cttctaccgc ttttcctatt gataaaataa taactggaac
    36301 ataacggtct ttatccaaac caaatgcctc tgccaactga tctgcttcga atccaccaat
    36361 tggatttgtt tcatagccat gatcacgcgc aactaacatg aattgcatag ccgctaagct
    36421 agaatcgact ttgactacat cgttcatttc ttctcttgaa aaactcttgt aataaggaat
    36481 gatcgcagct aattgttgat ctctgacttc ttttggcatc ttccctgatt cgtaagcttg
    36541 gttgtagatt tcttcgccat attcgtaaca ttgcatatcg ccaaaaatca tgaccattgc
    36601 agaagaagta tcattttgtc tcgtattgaa acggatcaaa ggacgcaaga tttcttttgc
    36661 ttcctcgctt tcagcgacca caaagcgcca tggtttcata ttgacagaag aaggtgcagt
    36721 cgtcgcttct ttgatcattt ccagcatttc ttcgtgtggg attttgtatg tttcatcata
    36781 tacgcgaacg gatcttctac cagtcataat tgttgaaaaa tcattttttt gttccattat
    36841 tattaacctc tttcgtttct gttgttatat tgcaaagttg ggtattcaat ttatctaaaa
    36901 gtgttagaaa ttgtttcatt tctacatcgc tgaaagccgg ggatagcacg gaacctgtat
    36961 ctttatggtc catttcacaa tgcttgattt tcttctgggc tttttctgtt agcgctacat
    37021 acacttcccg gttgttctga ggatttctct gacgcgaaac atagccattt gcttccaata
    37081 ccttcagatg acgagtaact gctccttgat caatctgcaa cttttcttgc aaactgcttt
    37141 gtagcaatgg attttcttct ttcagtctca tcatgatctc gtatctagta agacttatac
    37201 cgatattttc ttcaaagatc tggacgattt tttgatcggc taatttgatt tgatataaaa
    37261 ggtgactaat ctcttctaaa gacataattt cctcctttct aataattgac tcgtcaacga
    37321 ttgacttgtc aataatagaa taaaaaaaga aaaaagtcaa agaatcagct gtgtaataaa
    37381 aaaaacgctc tcaatagaaa agtatagaga aaagaaattt tttgtagaat gaacggaaaa
    37441 aatgataaag aaagcacaac gctaaaacca agattattga ttcttatcaa actatagaag
    37501 atagctcttc tgcagtagat aattttacac cgccaaaaat ttccgaaatc gccttttgca
    37561 agaatcccgt cgaatattag aaattggatc agtatgctta aacagtaata aaaaagttat
    37621 gacaagatgt tgtcacaacc ttttttgagt aaacggtgtt caaaaacttg catctgcggt
    37681 aataagttca accaaacaaa aaacatgaaa atctgttttt gtttggctgt tcttactact
    37741 tgatgcaggg cagctttttc actacctctt tttaataacc ataaaatcct attccttcat
    37801 atctccagcc catcggtgtt agccaatctc tttccttagc actcaaggta tagtgatggg
    37861 taccagtttt ttcatttgga ttaaatagtc gataaattgg cttggaacca ccagaataaa
    37921 aacttagacc ttcataacgc caaccgattt ttgttaaaga gttgatctca gattgtgcca
    37981 ttgtgtaatg atggtctcca ctgtaaggat tgtataaacg gtagacaggt tgtccgctgt
    38041 ttggtgcttg ccaagcaatc ccttcatatt cccagcctac agttggcaga taattttttt
    38101 ctccggcatt caatgtatat aaatgttctc ctgtatgacg gttatacaga cgatacatcg
    38161 gagaagttga agttggtgct gtatcatact gtgtcagatt ataggtttcg attacattgt
    38221 tcaatgtaga accataatta ggcgcagtgg cataacgacc tgttagccaa gccgtagcat
    38281 ctttgtaaga gttcgtattg cttttccatg caccggaata gtagtaaacg cctggctgaa
    38341 aagaagtcgt cttcaatacc gttacgttat cttgcaatga ttctttgtac gaaggatatt
    38401 ttctaaaagc ggcattcacc gtaatccatt gcccattcac atattccgaa gtaggcatcg
    38461 taacagattg gccattatag cttcctttaa tcccaaatag attgtaatta ggggcagaag
    38521 ccaaagtact gttcccccag ccactttcaa cgatggcttg agcaatcatg actgatgcat
    38581 ataagtcatt ggcacttgcc aaactttgtg cagttgaggc aatactatca atgaacgctt
    38641 gttgagaatt agcagatgag cgagcataaa aagcatagcc agtatagttt tcgaattcag
    38701 acaaatctgc ttgatgggta tgtgtcaatg tttgcccatc tgtcaattgg ttcgtgattt
    38761 cttctgcatt ctctggattg ttctgagctt ctgctgatgt actgtcttcg gtttgtgttt
    38821 gttctgttgt ttcagttgtt ggttcttcgg ttgaaattgt attttctgag acagtagctg
    38881 actgcaaagt actttctgta tcgttttcaa ttcctgtttc tgaagaactc gatgtctgag
    38941 ttgcgacagt ttctgtcgat tcttcagcta gcactgttgt gttggcaact gctagatttc
    39001 ctaaaagaat aacgctaaat aataaattct ttgctttcat cttttttctc ctttgaatta
    39061 tgaaactttt cctaaataac agtgtaacta aaaaagtaaa agcaagtcaa tgtccatatt
    39121 taaataacat ttgtttcttt ttaataaatt tgctttttct gcataaaaag acgtaaatga
    39181 agaatattct tctccattca cgccttaaat tgatggtcgt ttgaataact atttaacaag
    39241 taattctctc tggatattat ccgttatgga ttatacttcc tgtgtcgtcg gacggataat
    39301 catgttgctg acagacatcc ggtctggcgt ttctatcgca aatgctacag cagaagctat
    39361 atcttcagat gttaatcccc attctttttg cgcttcatga agttcaagag cagccttttt
    39421 gtcgctgatg gtttgatata attcagtttg aacagctcct ggagagatga tcgtagattt
    39481 gatgttattt tgccgttgct cttgacgtaa tccttccatg attgcacgga cagcaaattt
    39541 tgttccgcaa taaacggcgg aatcaggata aacaacatgt cccgccacag aatcagttga
    39601 aatgatatgc ccagattttt gtttttccat gattggaagt acggcggaaa ttccatttaa
    39661 cacacccatg atgttgatat caagcatatt ttgccattca tcacggtgac cttcaactaa
    39721 tggagcggtt ggcatgattc ctgcattatt gtacaataca tcgattcgac cgaacttttc
    39781 catcgctaaa tcgataactg cttggacttg tgcaaaatct cttacatctg ctgtttgaat
    39841 atatagctca gcttctggta aagattcttt tattgctttt aggcgatctt ctcggcgtgc
    39901 agctataact agtttagccc ctttttctgc taatagcttc gttgttgctt caccgattcc
    39961 gcttgaagcg cccatgatga caatcacttt ttctgataaa tcattcattc tattctcctc
    40021 ctgttttgaa aaatctcttg cttatactca ctatctctta tttcatctta ctgttcaaat
    40081 aatccgttcg acaggtttga cgattttggc agggattcct gcaacgatgg tattgtctgg
    40141 gacatctttc gtaacagttg cattcgctgc aacaatggca ttctcgccaa tcgttacgcc
    40201 tggcagaata gttgctcctg ctccgatcca agcatttttc ttgatgtgga ttggctttac
    40261 tgacaagcct cttcgttctt ttggatcggt tagatggttc accgtgatga ggttgacacg
    40321 aggaccaatc aatactgaat cttctaaaca gatgccacct aagtcaacga acatcacttg
    40381 acgattgata aagacatttt tccctatact aatatgtttg ccgaaatctg tataaaaagg
    40441 cagcagaaca gtcaccgtat catctacttt gtcactaatg atattttcta gccgtttgcg
    40501 gatatctgct ggttcatggt acccagtatt cagcgctgct aaaggctctt cgttttccgc
    40561 ttggatctga tggatcactt caaagagcgg atccgttact cgaatctctt tattgacgat
    40621 ttgtttttcg atgaggcttg tcattttgct ccctcctttt atccttactt tataacggac
    40681 ggcttatgat gtctaatact tataaaacat acttaactat tccttttaac tatacttagt
    40741 ggtatgcttt tttaaaaaca ggagggaaaa aatggatatt cgtatactga cgtatttttt
    40801 agcagtagct agagaaaaaa atatcagccg agcagcaaaa tctctgcata tcacgcaacc
    40861 tactctctca aaacaactga aagatttaga agaagaactt ggtgtgacac ttttcactcg
    40921 aggctcccga gagatacagt tgacggattc tggtcgatat ctttattcaa aaggaaaaga
    40981 aatcttgtcg ttggtagaaa aaacggcgaa caatttaaca aatgacgcaa tcatctctgg
    41041 agaaatctac attggcggag gcgaaactaa agcaatctct ttcatttctg atagccttca
    41101 ttcgttgatc aaggcgcatc ctgatatcca gtttcattta tatagcggga atgcagatga
    41161 tatcatagac aaactggata gcggattact tgattttgga ctcgtaatcg aaccgacaaa
    41221 caaacaaaaa taccagtcat ttcgactgcc agcccaagat cgttggggac ttttgataca
    41281 tcggaatcat cctttagcaa agcaaacagt catccatccg caggaactga aaactgttcc
    41341 tttgatcatt tcccggcaaa cgtcagtgga cagtcaacta gctgaatggc taggtttcag
    41401 catagatcag ttatcgatcg tcggaacata taacttattg tacaatgctt ctttgatggt
    41461 cgataacggg acttttggtg cattagctct agatgggatc atcaatactt atgggacgga
    41521 aaaagtattc atcccactcg atccgccatt acaagcttca ctgaatctca tttggaaaaa
    41581 agaccagcct ctttctcctg ctgctcgtaa atttttagat gtcctaacgg agaaatacag
    41641 ttaagtagag tttttttcct tcgctttcag ctttttaaca acgaataagt taagcaaaag
    41701 atccaatgat taaataacat aagtttctta atttgttcct tccttttatt tctgataatt
    41761 ccagtaaatt actataatta taaaaaaaga attgacaaaa cataactgtc tatataaaat
    41821 tgattacgtt aacaataatt cgtttcatgt gactagatca tactaggcat gaaaaatctc
    41881 tgaggaacat ggtttcccta ggaggttttt cgtgtctatt tttgtttggc cgcaatcaac
    41941 tggtctagca aacgtgaaaa ccaaatcttc aaaaagggtg cgcgcatgaa aatcaaaaag
    42001 atctttaatc aaaatgcagt attggtagat gacggtggac aggaaaaagt agctattgga
    42061 aaagggattg gatttgacag aaaaagaaat gatttgattt ttgatcgtga tgtcgaacgg
    42121 atatttgtca tggaaccaga aggtcaaatc aagttgcaaa atttacttaa tcagattgat
    42181 gaacgctttt tatttgcagc agaaacgatc attgatcatg cggaaactgt tttgatggaa
    42241 aagttgaatg aacatgtatt gatcgctttg accgaccaca ttgctttttc tgcggagaac
    42301 attaataacg gtatcgtcat ccgcaacaaa cttttaagag aaatcgaagt gctttacagt
    42361 gatgaattca gtattgctca atgggcagta gagtatctga caaaagagct tgggattcca
    42421 tatacttatg acgaagcagg atatatcgca atccatattc acagcggacg aagcggtcgg
    42481 aacaataacc atcgcagtat ccgtgaagtg acgattgtat ctgacatcat ccatttagtg
    42541 gaatcagaat tggaaacaga tatccattca gaaccatttt cactgaatta ttcaagactg
    42601 gtgaatcatt tacgactgct tctacaacga acccatgcgc aacaatatgc tgttttagac
    42661 acggaaatcg tagaaatggt caaacgaaaa tacccagaaa gctataagat ttctaaaaaa
    42721 atccgtgtgt tgctgaccaa agaataccag ctcgcgatca cgaaagaaga actgggttac
    42781 ttggccatcc atatcgagcg tttaagaagc gcaatcgtac aaacaaatgt taacaatcat
    42841 gaggaggaaa aaaattgaaa gcatatctac aacgaatggg acgttccctt caacttccag
    42901 ttgcggtctt acctgctgct gctttactag tagggatcgg aaactggtgg gcatcttata
    42961 gtaacgacat tgtcgctcat tttttacaag ctggcggtaa cgcagtttta ggtcaattac
    43021 ctttgctgtt tgccgttggg ttagcactag ggatgtcagt ggacaaatca ggtgctgctg
    43081 ccttggcagg tttggtcgca tttgaagttc cagtaaatgt tttaaaaact gattcagtag
    43141 ctactttatt gaatatcaaa ccagaatcag ttgatccatc ttttgctcag atcagtaacg
    43201 tatttattgg tattattgcc ggattgattg cggcagctct ttataatcgt ttccacgaag
    43261 caaaattacc gatggcttta tctttcttta gcggcaagcg tctagtgcca atcttggctg
    43321 cctttgtcat gttgctgatc agtgcagtgc tactgctcgt atggcctccg gtttactctg
    43381 ctttagtttc ttttgctaaa ttcattttga gtttaggctg gggtggtgca ggtctttatg
    43441 gcttcttcaa ccgtttactg atccctacag gattgcatca agcaatgaac tccgtgttct
    43501 ggtttgacgt agcaggaatc aatgatattg gtaaattttt ggcttctgaa ggaacaaaag
    43561 gcgtaacagg aatgtatcaa gctggatttt tcccaatcat gatgttcggg cttccagcag
    43621 gagcatatgc tatttacaga aatgcacgtc cagaaaacaa agcaaaaaca gcttccttga
    43681 tgttagcagc agcttttgct tcattcttta ctggtgtaac agaaccactt gaattctcat
    43741 ttatgttcgt tgcttggcca ttatatgtct tgcatgcaat tttcactggg atttctttag
    43801 cagtgagtgc attcttccat tggacagcag gatttgcctt tagtgccgga tttgtcgatt
    43861 acttcctatc tttgaaaaat ccgattgcga atcagccatt gatgttgatc gtccaaggat
    43921 tagtaacagc agtgatttac tactttggct ttaactttgc tatcaaaaaa ttccatctga
    43981 tgactcctgg ccgcgaggaa gcagatcttt cagatgacga tactgcaacg actaattcag
    44041 acaataaata tgcggcacaa gcaagcaaaa tctacgctgc attaggcgga gccgacaatg
    44101 taacttcgat cgataactgt actacacgcc ttcgtttgca agtcaaagat acaggcctga
    44161 tcgatcaaaa taaaatcaaa gccactggcg tacctggtat gaagatcatc gacggaaaaa
    44221 atgcgcaagt catcgtaggt acagaagtac aattcgttgc agatgagatg gcaaaactac
    44281 atggtggtgc tgcaacacga cctgcacaaa caaacaccgt ggttaaaaca gaaacatttg
    44341 ccggtggaac gatcgaacaa gatatttacg ctatcgcaaa tgggaaactg attccaatca
    44401 ctgaagtttc agatgatgta ttcgcggaaa aaatgatggg tgacggctat gctgtattac
    44461 cagaaaatgg agaaatcttc tctccaatcg ctggtacgat cacaaacatt ttcccaacga
    44521 aacatgctgt cggaatccaa acagatgctg gtatcgaagt cttgcttcat atgggcatca
    44581 acactgttga cttaaaaggc gaaccattca ctttatatgt aaaagaaggt caaaaagtcg
    44641 caagaggtca attgattgct ttagttgatc ttgccgctat tcaatctgca ggcaaaaaca
    44701 cggatatggt cgttgtattc acaaatggcg acaaagtcca aagcttagaa atcgaacccg
    44761 cacgtgatgt aaaagcaaat gataaaatcg gctctgtttc caacaaagcc tgagccatcg
    44821 tcttccccag acttttttat cgataaacaa acaggagctg agacaaaaat cttgtctcag
    44881 ctcctttttc cgaataaaaa cggcgttctc ctgccaactc cgcggtaata agtccagcca
    44941 aacgtaacat atgaagagct attttttatt tgactcttct tattacttga tgcagaacag
    45001 cttttttaca acctctgata aacggtgttc aaaaaccagt ttatcggtat gaagtcaaat
    45061 ttatgcaaaa atgtggaaag ccattttcac aaaatttttc ttcatactca aaactaatcg
    45121 gctttttcac aacctcttta tacattattc ctgatccaat atttctttta atgctgcttc
    45181 aagagaagtt aatggttttc ccaatacttt ttctaaatca tcagaggaaa tagctaactg
    45241 atccttttta atatcatgtt ggatcgctaa aaacatgtca gctgcttctt gagggaatcc
    45301 ggcttctttt agattttcga caaacgctgc atcacttgct tcaataattt ctaacttttt
    45361 acctgtcgca cgctctaatg ccttggctaa ttcttcatag tcagttagat tgccggataa
    45421 ttctaagatt tctggagagt cactttctac tagtactttt gctgcagctt ctgcatattc
    45481 acgtttcaat gcccaacctg tttttccatt tcctgcagca taaacgaatt gaccagtttt
    45541 taaagcctgc ccaatgatag gtaattcatt ctctacatac caattattcc gtaaaaatgt
    45601 atgggaaata cctgaatcct taatcatttt ttcagtgaat tggtgatctg gcgctaatac
    45661 actgatcgag tgatcggcat ctgcaaaact tgtataagcg atataagata ccccagctgc
    45721 ttgtgctgcc ttcactacat tcttatgttc ggcttgacga tttcctggaa cgccagagac
    45781 aaaaagcaat cgatctattc ctttcaacgc ttctttcatt gagtctaaat catcataatc
    45841 accaatacgt atattaaatc cagcttcttt cagtgcttct cctttttctt cactacgtac
    45901 tagtacatag atttcatttt gtggaaccat cttttttaag aattccagcg cataaccacc
    45961 aaaacctcca gttgctcctg ttactaaata atgcatattt gccactccct attcttttta
    46021 tttgttaaga tttacagctg tacatttata aattacacct ttatcataaa gagtgtggta
    46081 agataagtca agcaaaaaaa atgaaaggat gacaactgat gaagtattct ctgcaattta
    46141 gtgatgcaat ccatgtactt gcctatattg aaatgtttca agatacccga tcactttcta
    46201 gtgagatgat tgccagtagt atcgaaacaa atgcagcaaa tgtacgtaaa atcatgagtc
    46261 agctcaaaaa atccggtttg atctttacaa aaaccggaaa accgcagcct tcccttgcca
    46321 aaagtccaga tcagatttct ttactagatg tctatcgcag catcgaaggt aatacacagc
    46381 ttatccaagt cgatccaaaa acaaatccaa attgcgtcgt tggtgcgaat atccaagaag
    46441 ctttagaaga aagctacgct cggctgcaga aaaaggcaga agaagaaatg gctaagatca
    46501 cacttgatag tctgatacat aaaatagccg ttttggaaaa agaaaaaaga ccagaaaaca
    46561 caggcctcgt agagagattt ttataattct ctctactgtg cctgcatttt tctgatcgga
    46621 atcgtctaca taaattcttt tattctccgg gggacaaaag ccttgtcatg cccctttttc
    46681 ctctctaatg ctcaggtcaa agcgctgaag tcatgacgtc ttgataaacg gtgttcaagt
    46741 agctgaccct cggcattagt tctgaaaaat gcaaaatatg gaaagctatt tcccattttt
    46801 cctctctaat gctcaggtca aagcgctact ttcacgacct ctatattatc atggtttatt
    46861 gactaccggt atccagattt ctcccatata tttcccgttg gtaactccca tttcgactac
    46921 agcatttgga cccccaacat aggcgtaatc ttctacttct cttaaaacat gcccaaaggc
    46981 cgtattcgtc aaatcatttc ttaattcttc aggcgattct gctaaagcga ttacaacaag
    47041 atacgttcct tttgggaatg ggatgattcg ggtagtgttt ggaacatcct gactcgtttg
    47101 aacaccgaca tagtacatca ttttcccgtt atagggttcg ttgactgtaa agaaataatg
    47161 attttcagca gcttgtttca atgcatctat tgttccatta ccgataactt tttgctcaaa
    47221 tgcttgtttc tctgcagcca aaccgacaaa atccgtattg ctactcttca attccacacc
    47281 aatacccgtc accgtaaaat ctggtttttc ttcgatccta tactttgcca tttctcccac
    47341 cctttctatt atattaagtt catcttacat aaaattaaag ggaaatgaca cgattacgct
    47401 cttttttcta gggatatttt tattttttaa ctattttttt gctttcactg caatccaaat
    47461 ctcactgtaa acattactga aatcctctcc ccattttgta aaagagatct cgggagcttc
    47521 gactaactca taatctgaag aaggaagcca ttctgcatag attttccccc aagtatcttg
    47581 taaagtttgg ggaaatggcc ctttattagg aaaaatggcc caagtgtgtt cttctatagc
    47641 aagcatttct aaatcagcgt acggattttc ttttgtcgta gcaaagccaa tcatgtgcat
    47701 caaactgccc ttttcttcca agcgggctcc atcaaatcca taagaaacgt tgattacttg
    47761 atttggatat aaatctccta acttatgcat ctcttccctt tgagtctccg tgatcgtctg
    47821 cgctaacttt tgtatttctt gattgactcc ttcaaatttg acaggaactt ttttcgccac
    47881 acctgccaca taaaactgtt ctttcttttc gatacgaaat tccatcgatt ttcctcctct
    47941 gacatcaata aaaaatgtca agcgtggaaa agatttttgt atgccgtatc tgctgatttc
    48001 tgaaggcaga tatcctgacc aatcacgaaa agctcttgaa aaaccttcga ctgtttcata
    48061 tccatatcta aacgccgttt ctgtcactct tgatccatct aggagatcgc gattagccaa
    48121 agccaatcta cggtttttga tatagtccat tagcgacatc cctgcaataa acgtaaagat
    48181 gcgttttaat tgatattcag agattcctgt attttcggct attattttat aggaaagtcc
    48241 ttctgtcaaa tgttcttcga tgtaatcgat caattggttc agatctttaa gcatatttat
    48301 cttcctcctt gcttgccttt ttatcatatt atcaaagaaa gtcaattact tgatgaatca
    48361 ggaaccaatt tagtcagatg tagaaaatgt tgtatggatc taaagaaaat tctcaagcag
    48421 cgtttcgtaa cggataattg gtattatctg cctatctatg ttttaccttt agaaaaataa
    48481 attttgggga agacacattc taagtaaaca aagcggtaaa aatatctttt attcccttag
    48541 ttaatccgct tactacctac tgtacaaagc aaattgttgt tcaaactgct gatcagaacg
    48601 ctgcttggat ttttctttgc gatacatcat acctctttta tcaattggaa caattattta
    48661 tttctaacaa aatcacttca tttttgtcag ataaataaaa aacaaaaaaa gttccatctt
    48721 taaaacacgc tattctttta ctttttctca cattctctaa gaaaagaaaa ggagaatgaa
    48781 tttttgacaa ttcgagcaaa agtgattatc aaatatgtaa aatcagctac ttttttctta
    48841 aacgtttctt tatactaaat ataaataagt gtttcatgaa aggagaacta gatgaataat
    48901 tggcttgagt attttcctga aaacgttttg gaacgcggtt acagctatca tctgcacggt
    48961 tttgtccgtc atttgaacta tacaagcaag tacttgtccg caacagtttc tggcacggaa
    49021 gattataagg tagtgatcac ttgggatgaa aagaccaaca tgacttgtga ttgtctatat
    49081 gccatagaag gaaaaaaatg caaacacatg gctgctgtct tattcgctta cgaagaacgt
    49141 ccaataaaaa aatccaacta ctcactttcc gaactctcgt cccttgtttc ctccgctagt
    49201 tcctctcttg tacgggagct tttaacagag attcttatag aacatccgca atttatcgaa
    49261 cgcttcaaag ttaagatgcc atttcatgca ataaattatt cagataaatt aacaactatc
    49321 atccataaat acgatcatat tatcaagaaa aacaaaaaca ggaaaacagc gaagttcatc
    49381 atggagatgc gaaagttcat ccaagaagca gttgaatcgc tcattcagca aaatgcctat
    49441 cttcccgcat tcgaattgat caatgaagtg attgctactc tcgaaacatt ctattgggag
    49501 cctgaggatg aacgaaccct tcttctgata gaggactgtt attatttatg gaaagaatta
    49561 ttagcagaag cacctcatgc agaaaaaagg caaatgttct cttggttcgt ctgccaagtc
    49621 gatcatactg acgcctctta ttcaaaacgc tactccataa aaatcctgaa agaagacttt
    49681 cgggaaaaag aattttctaa ccaaaagaaa aaaatagata aaaaaactaa agaaacggta
    49741 aagaaagacg atttcaatga aaaatagcaa gtaaacgata acaaccatcc taaatccaca
    49801 agaaatacag cagaccttgc tttaaaaaca cgaatcatgc aaaggatgaa gtaaagaaga
    49861 cttaacgcgt acccaatatt agtcatttga ctattaggta cgcgttttgt tttataagtc
    49921 atgacaaatt tccttcgttg ctgggtagat tttccggcat tgttgataga ttttatgata
    49981 cttttctaca ttttgtaaga gaggttccac tgcatctgta tattggataa aattctgaca
    50041 acaactttca ggtgtgtcga accactctaa tctttaaaga taaatattaa aataacgatt
    50101 aataatatta acagtcttta tttttcacca ccaaaaacct atctttgcat atactaaata
    50161 gacgtatata cacagatagg tttgtcttgt gaaaaataat gattatcctt accaaaaaat
    50221 gtttgttttt ctacatgttc gtacttatca aaaaatatga tccttattga aattactggc
    50281 tatttatatt ccaacgactt cttgtgttaa tttttgttat ctcatagtaa attatgtttc
    50341 attttcactt aatttagttg tcacgtgttc gtcaattcgt aaaaacggta aatatataaa
    50401 cacacttgcg gctataatta atatttgtaa aacagctgct cgccagtctc cggctgttgc
    50461 taaaaatccc gatataactg gtggcgtagt ccaaggaatt tgcactacta catgattaat
    50521 taatcccaca gcagttgcaa aatatgctac tgtcaaagaa aaaatcggtg caagcacaaa
    50581 gggaataatc agactgatat taaatacaat aggcaatcca aaaataatag gttcgcttat
    50641 attgaatata gcgggagtta ccgataattt agcaaagtcc ttataatctt ttctacgact
    50701 gaagataaat attgcaatca ataaagcaat cgtatttccc gatccaccca ttaccgcaaa
    50761 ggtatcttta aaggccatat tgataatatg tggaatttgc tctttgtttg cataagctgc
    50821 catattttct tgcatattct gaatcaaaaa cggatctaac aatgaacctg atattactgc
    50881 ttgatggata ccaaatccaa aaaatagatt agcaatagat gtaattaaca aaaatcctgg
    50941 taaaccagtt gtaattttac ttaatggctc tgtaactatc gtggtaatta gcttatttat
    51001 atccatatta aacaattgaa tgattataaa tgacaataaa gcaaacgagg taatcgtaat
    51061 aatgattggg attaatacat taaaagactt taaaacagcg ggaggaatat ttccggatag
    51121 ctgaatctgt agtttcttat tattagaaag tttaataaat aattctgtag ccgctaatcc
    51181 aacaaatata cctacaaaca ttcctgaagc tcctgtatag gatataggta tcacacccgt
    51241 cacttcaaca gctttatccg cattttcagg agtaaacatc atctgcatag gtgttaatac
    51301 aacaaatgtc gaaatagcaa ctaataccgc tgcaatatga tttgtataat ttctgcttgc
    51361 acacatatga taagatactg ctgctacaac taataatgta gttatgctta atgtaccatt
    51421 ggcaattgaa gtaccaattt gttgtaacgt ggtcaatgta ctagtatcga caatttcccc
    51481 catgaatcct gttggttcta agatcactga attaataagt gtcacaaatc ctgctaacat
    51541 cataaatggc ataaatgtag caaacgcatc tcgcaaagtt cttaaatgga tctgattccc
    51601 caacttagtc gcaactatac caaatttttc aaatacaatg tccttcctgc tacttttgcc
    51661 catatttttt ctcctttatg cttattgtaa tcgattactt tttccaccca aataatacat
    51721 cacttatcaa aaaaagtaaa tacttttaat taaatatata tatctttttt agtataaaaa
    51781 aataagctct gaaaaaataa ttttcccgag cttatttttc aacaaaaaca gcttcaaaaa
    51841 caaagtcttg atataaatgt ctcgataccg aatactcgaa aggtttacca gacgatagag
    51901 caacaacttt gtctatctca atcataaatt cacctggatc tatttttaat atttttgcgt
    51961 caaattcatt cgcagtatct cctttgatac gtatgtgcga agtctttatt tccagaccca
    52021 gttcatttgt gatataagca tatacagatt tctttaaatg ttttggttcc aagcctggta
    52081 tgactgctaa tggcatgaat gtttgctcaa tcgagtatgg ttgacaatca ataaaacggc
    52141 ttcgtacaat attatataca tagtctttta cactgatact taatttatca gctacttttt
    52201 tcgatggaag ttcaattgag aaagtatgga tttctgaatc tacgtgttct tcaccaacca
    52261 cgctaaacaa accttcgttt ggaccaagaa ctcgtgagtt atcaattggt gttctgacga
    52321 cttcagtccc atacccactt ctacttcgca aaactccttc ggaaacaagt aagtctacgc
    52381 ctttctttac tgttaacttg cttgtatgat aaataactgc caattcatct cctgtaggaa
    52441 gtttctcacc aattttatac tctccattta caattttttt ctttatatct aagtaaattt
    52501 ccatatactt cgcaattttt ttcaatggtg accctccaat caaaaacata tcttcatcta
    52561 tactttaaca taaataaacc gataaaatcc acatcaaaat tatacacaca ataaaaaaga
    52621 tatatatatt taaacaaaac tatttacttt aagttgtaag cggtgttatc attaagtgga
    52681 aacaggaggt aaaaaaatta tgacaactat gaaaaagcta acttttccag atgatttttg
    52741 gtggggttcc gcttggtcag ctgagcaagc agaaggaaaa ggagaaacag gaaaagcaga
    52801 gactatctgg gatcgctggt ttaaagaaca accttaccgg ttttatcaac gaatcgattc
    52861 ctctattaca accgatcaca tccatcaata taaagaagat atagaactaa tgaaaaaaac
    52921 tgggcataac tcctttcgcg tttctatttc ttgggcacga atgtttccaa atggcggtac
    52981 cggaaaagta aatcccaaag ccatttcttt ctatcgaaaa ttattttccg aaatgaataa
    53041 taacggaatc aaagtatttg ccaacttata tcattttgac atgcctgcta ctcttcaaga
    53101 taaaggagga tgggaatcac gtgaagtagt tgatgcttat gttcattacg ctgaaacttg
    53161 ttttaaagaa tttggtgact tagtttgtca ttggtttacc ttcaatgaac cacttgggcc
    53221 tattttaggt acttatcttg aagattttca ttatccaaac ctcattgact ttaaacgcgg
    53281 agcgcaagca gcattcttca ctatacttgc acacgcaaaa gcaatacaag tatttaaaga
    53341 actagctctt cctagtaaaa taggcgttat tctaaatttg agtcctactt ttcctcgtag
    53401 tcaaaatcct gctgatgtga aagcagcaga aatcgctgat ttattttata cgcggagctt
    53461 tttagatcct atggttaaag ggaatttttc taaaaagctt atcctgttat tacaagaata
    53521 taatcagctt cctgaagatt atacgaaaga agatcttgta tgtatctacg aaaataccgc
    53581 tcaaattctt ggactaaact attacgagcc acgtcgcgtc aaatctcgtt cttctatggt
    53641 ggatacatct agtccatttt taccagagtg gtttttcgaa ccacatatca tgcctggacg
    53701 acgaatgaac gaataccggg gatgggaaat ttacgaaaaa ggaatttatg atttgtgcat
    53761 ggatatcaag aaccattatg gaaacatcga agcatttatc tcggagaacg gaatgggcgt
    53821 tgcggatgaa gaacgtttca tggatgatac agggcaaata attgatgatt atcggattga
    53881 gtatatcaaa gatcatcttg cttacttatg gaaggcaata aatgaaggat gtaacattaa
    53941 ggggtatcat ctttggacat ttattgattg ctggtcatgg atcaacgcct ataaaaaccg
    54001 atatgggctg gtttcactcg accttactac tcaaaaaaga acaattaaaa aaagtggcga
    54061 attttacaag caattaagtg atacaaatgg atttagatac gatgtagact tgttagttga
    54121 ataatattct aaaattccac ttgaagtgaa cacattagaa aacaagaata atgagaaata
    54181 ccaataacaa atactttttc ttcatacttc atttatactt gtcagtataa gagactgtga
    54241 caaatgtagt gatcctcaga agttaggtct ggaaatctaa cttgagaaga tttttttaaa
    54301 ctacgtccag tcttttttat ttgtttaaaa ttgaagttga caagtcttca ttaaaaattt
    54361 agaatattca caagtttgac gacaattatg aaaatttatg tcgtgctaaa ggagttttgt
    54421 tattggatga aaagttagat ataaaaactg cgatcaaaga cacgttacct accgtttttg
    54481 gttatatcgg tattggactt gcatttggta tcgttgggaa agctgccggg tttcatccgc
    54541 tggtcgtcac gttgatgtcc ctgctgatct atgctggttc tgcccaattt atcacggtca
    54601 gcatgcttgc tagccatagt ccactgctct ccatcgttct ctcgaccttt ctagtcaatt
    54661 cccgaatgat tctgatgagt atgacgattg ctccttattt caagaaaaat cggctgcttc
    54721 aaaatctgct gattggcaca ttgctgacag atgaaagttt cgcactggga atgaataaat
    54781 tgaactatac tggacaaaag ttaaactttc gttggatgaa tacagctaat tggatttctt
    54841 atttgacgtg ggtgggttct tctcttgttg gcgcactttt agggaatttt attactgatc
    54901 cgaaaaagtt tggtttggat ttcgccatcg tcgctatgtt tatcgggtta ctttatcttc
    54961 aagtcatttc tgatagaaac acctctaaac gattacagtt gatcctgatt ggtctcactt
    55021 tgattctggt atatgtcgga ctgattttta tcccaagtaa cttagtgatt ttagttgtaa
    55081 cactgattgg gtgcggattg ggggtgtgga tcaaacatgc cttcttttga gtttacattg
    55141 ctgactattt taggttgtac tttagcaact tggctttcta ggatcctacc atttgtctta
    55201 ctgaaaaaat tcgatttgcc gcaaccgttg cttgagtatt taagttttgt cccgatcgtc
    55261 attatgtctg ctttatggtt cagcagtcta tttacccaaa atatcggaca tctgccgcag
    55321 atcaatctcg aaaatgcttt ggcttcagtt cctacacttt tagctgctat tctttcaaaa
    55381 agcttattgg tgatcgtctt ggtaggaatt ctttcccttt cgttgattcg cttattataa
    55441 ataaaaaaac aagagcttat ggcaaattac tgtcatgcgc tcttgttttt atcaaagtca
    55501 aataagaaaa aaatcggcgt attcttgctt atccgttgaa cggaccacta acaacgactt
    55561 ttcctaatat atgtcctgtt tctacgtctt tggttgcttg tttcaagttc ttcgcattca
    55621 tcccatctga atagaccttg ctcaatgtac tgcggataat cccttcattt gctaaagcag
    55681 caatgtgcgc taaagcttct ccttgcgttt cgatttcata gttatagtct gttttagcga
    55741 acatatactc ccagtcgaag ctgatagatt ggtttttcca aacacctata tctaacggct
    55801 ctttgatccc tacgatcgtt cccacatgtc caaatggtcg gacaaggtgt ttgatttgat
    55861 caaagtagcg ggtaatatcg aataatacag cgacatactc tacttgatcg attcctaatt
    55921 cacgaaaact tttacttaag tcgtgatgat aatcgattgg atagtctaca cctgtttttt
    55981 tcagccattc aaaattcttc ggacttgcag tcgcgtagac ttctaggcca gcccaatgcg
    56041 ctagttgatt caaaatcgaa ccgacgcccc cagctccgtt gatcactaag atttttttcc
    56101 cgcggttggc attttcttct ggagtcagac caaatttttc aaataacaat tcataagcgg
    56161 tcaatgaggt caacggcaag gctgcggctt cttcatctga aagatttttt ggtgcaagag
    56221 cgacaatacg ttcgtcaacc agttggtatt cttgattgct tcctgctctt gtcgttgtac
    56281 ctgcgtaaaa aacgcgatcg ccgacagaaa atttttgcac ttcatcacct acagcaacaa
    56341 ctttcccgac tccatcaaac cctaagatac gtaaagcatt tcttatcccg ttatcttggc
    56401 gaagtttcgt atcgacagga ttaacagata ctgcgttgac ttttaccaga agatcccgtg
    56461 aactgggtac aggtatctgt tctttttcat caataaagct ttttggatca tcgatcgaca
    56521 acccttcata aaaaccgact accttcatta attccttttc tgacatacga attcctcctt
    56581 agcttcatca agttggagca tccatacagt tttcatttca atatatatag tatgctattt
    56641 gttccacttt tattcatcat aattattctc ttacttaaag taaactattt gcactttttt
    56701 ggttaaaaaa gaggttgcga caagactttt gtcacaacct cttttaaacg gtgttcaatc
    56761 aactgaccct cggtattcgc tcaaaaaacg acaaaatatg aggagctatt tttgtttggc
    56821 tgttcttatt acttgatgca ggacagcttt ttcacgacct ctaattcacg gtgttcaagc
    56881 aagaaatcat tgttcactga aaagcgttgg atgttctttt tcaaaggtgt ctaagatctc
    56941 tcgtacttca tcagtagatt tcgtattcat caactgaact cgcaagtcat ttgctcctgg
    57001 aaatcctttg atatagattt taaagaaacg atgtagtcct gtgatggaac gcgggatgat
    57061 ttctgaataa tgatcttgca gatctagttg cagacgaagt agtccgatca gttctttttc
    57121 tgtatgttcc ctcggttctt tttcaaatgc ataaggattc ttgaaaatcc cccggccaat
    57181 catgatgcca tctacgccat atttttcggc caattcttct cctgtctggc gatctgggat
    57241 atctccgttg atcgtgatca gtgtttgcgg agcgatttgg tcacgtaaag caatgatttt
    57301 agggatgact tcccaatgtg catctacttt actcatctct ttacgcgtac gcaaatggac
    57361 agaaagattg gctatatctt gatgaagtag gtgactgatc cattcttcca tctcagacat
    57421 ttccgtatag ccgatacgcg tcttgacgct gacaggcaaa ccgccagctt tagccgcttc
    57481 aatcaattgt gccgctactt ctgggcgaag gatcaagcca ctgccttttc cacgttctgc
    57541 tacattaggg actgggcagc ccatgttgat atcgattcct tggaagccca tttctgccat
    57601 agcaatgctc atttctctaa aaaattctgg cttgtctccc caaatatggg caaccatcgg
    57661 ctgttcatct tctgtaaagg tcaatcttcc tcgtacactg tcttttccat ctggatgaca
    57721 gaaactgtct gagtttgtaa actcagtaaa gaagacatcc ggtgctgctg catgctttac
    57781 gacatggcga aatacaacat ctgttacatc ttccattggt gctaaaacaa aaaaaggctt
    57841 tggcagctct ttccaaaaat tagtagtcat ttatttgtta ctctccattc tatcaagcag
    57901 tgaagtcgca atctttctca aaaacattgt cattatacta ggaaacaaaa aaagaagcaa
    57961 agtgaacagc ttaaaagatg tttctgattg agcaaaaaaa gactgtgaca aaaatcttat
    58021 cacaattcct tttccggaat aaatggtgtt caaaagcttg catctgcggt aataagtcca
    58081 accaaacgaa aaataggagg agctattttt gtttggctgt tcttattact tgatgcagaa
    58141 cagctttttc acaacctctc cactaaacgg tgttcaatca gctgaccctc ggtattagct
    58201 caacaaacga caaaatatga ggaactattt tcccgtttgt ttctctaata ctcaggtcaa
    58261 aacgctgatt tcacaacctc ttttctaact tctcaatcct cttcctttat tgatcaaata
    58321 ccaagtaacg gcgtacaaaa caacgatgaa tagcacaagt acgatcatcg atgttgctac
    58381 tggcacatcg attgttccaa ggaatccgta acggaaaccg gagatcatat aaacgatcgg
    58441 attgactttc gaaacagctt gccaaaatgg cggcaacata gagatggcat agaaaacgcc
    58501 acctagataa gttaacggct gcaagacaaa tgtaggcacg atcgatacat cgtcataaga
    58561 ctgagcaaaa atcccgttga gcagtcctgc taacgaaaac aagattgccg tcatcaataa
    58621 tgtaatgatg accatgaacc atgaatacac atgaagtggt acaaagaaca aggaaatgat
    58681 cgttacgaga gagccgacga gaatactgcg acccactccg ccaatcacaa agccccaaat
    58741 gatcacatgc gtaggaacag gcgctaccaa gatttcttcg atgtttttct ggaatttttg
    58801 agagaagaag gacgaagaaa cgttggcata cgaactagtg atagcagaca tcataatcaa
    58861 accaggcacg atgaattcca tatatgaaaa accacccatg tcaccgatcc gaccaccgat
    58921 catttttccg aaaataacaa agtacaagga tgtcgtgatc acaggcggaa ccagtgtttg
    58981 gacccagata cgcaaatagc ggttcgtttc cttgatagct aaacttttta atgcagtaaa
    59041 ataaagatta aacatttttc tcctccgtat gattattttc ttctgtgatt ttcaaaaaca
    59101 gttcttctaa acgattcgac ttattcctca tagaaagaac tttgattcct tgtcttgtca
    59161 gctgatcgaa gattccgttc accccttgat ttcgttcaac ttcgacagcg attgtctgtt
    59221 catcttcaaa tgtacaagcg tatccttcaa tcacaggttt ggtctcatat ggcgctaaat
    59281 caaagataaa tgtttcaaat tgcagtttgg atagaagctc tttcatacta gtattctcga
    59341 tcagttcacc tgattggatg atcccgatgt tgcgacaaag catttcagct tcttctaaat
    59401 aatgcgtcgt caaaatgatc gttgtaccat tttcgttcaa ttctcggaga aactcccaca
    59461 tctcgcgtct caattcaata tcgactcctg ctgttggttc atccaagatc agcaaacgtg
    59521 gttcatgcat caatgcccta gcaatcatca gtcttcgctt cattcccccg gaaagcatac
    59581 gtgcacggac atttctcttt tcccaaagat tggactgctt caaatatttt tcgctgcgtt
    59641 tcatcgcttc tttgcgagag acaccgtagt agcctgcttg attcacgacg atttgctgga
    59701 ccgtttcaaa gggattgaaa ttgaactctt gcgggactaa tccaatctgt tgctttgctc
    59761 ggactagatc cgtatccaga tcataaccga agacattcac ttttccagat gttttattga
    59821 ctaaagaagt aatgattccg atcgttgtag acttcccagc tccatttggt cctaatagcg
    59881 catagaaatc tccttctttc acggaaagat cgattcctcg caatgcttcg accccacttc
    59941 cataaacctt tttcaaatta tttatttcaa gcgcatttgc catttatttc ctatctcctt
    60001 atagacatga ttctttattg tatcatggaa tacagcagat gaaaaaacaa cttggttcca
    60061 gcgaaaaatc aggttgctgg gctcaggaaa atctcctaat gaaagacatc aatcggacga
    60121 agtcagacaa ccttttcaga aaaaacaaat cgaagataaa ggattactat tcatatgtgc
    60181 aacaaaagga gcgtgagaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    60241 aaacggcgga agcaaaagcc aagatttttg atccaataaa tactttcgat cacttcgcct
    60301 atcccaatac atgccactgc cgcttttcat caaataacgc ggcttccgtt tttgcggttt
    60361 ctactaaagt ctctgggtaa tccaaaactt tcagcaaggc aatcgcattt cgggtccttg
    60421 aaggtccttc ttttaatttg aaatcaaaac tgattccttt tccttctgtt acttgttctt
    60481 caaagtggac attctcacaa taattcttta aaatctctgt caattcgata tcatgtgtgg
    60541 cgacaaatgc taaagatgga tactccgcaa gccaccgaac gactgaagcg gaagaagcaa
    60601 tccgttcgat cgtatttgtc ccttttaaaa tctcatctac gaagcagtag cagcgttctt
    60661 tggaagccac ttggtccaac aaacgtttga tcgatttgat ttctgaaaca aaataactgt
    60721 cgccttcaaa taaatcatct tcgattgcca tggaagtcaa cacatgtcca ggttgcaggg
    60781 taaactgttc tgcaatcgct gtttggatcg tctgtgctag aatacagctg atggctatac
    60841 ttttgacata cgtagatttt cctgatgcat tggaacccgt gactaatgta ttcttctgcc
    60901 agttgaccgg attcactacc gcttctttta acaatggatg atagatatct ttcgctgtca
    60961 caccgccctc agtaaactct ggtcggcaag tgatgggcat ataagtacgg taattcaata
    61021 ttgcagcggc tacttctaat tctcccagca attcccacag cttgatggct tcagttgaat
    61081 gcttttctaa tttagtgaga acgaaatgat aggagataaa tggcagcatg aacatgatgt
    61141 tgacatattc aaaaatcaac tctgcttcac tcccactctt catacgaaat gaaaaaccta
    61201 atttagggat gtctcttaat ggtttagttt gctgtttcag ctcttcttga agtggtgtcg
    61261 gcagttttgc tagctgattg gcggctgaaa tcgttgaaac caaataactc atgctattca
    61321 gctctgtttc tagtgtttgt ttcttcacta gataatagac cgtattgaat actaacccac
    61381 cgattgccaa atagattcct agtacttgtc ctaaaagaag caaacccaaa cccaccagcg
    61441 gaagcaagcc aagaaaaata aataaaccta cgcgtccaat ctttttactt tttccatccg
    61501 ctagatattg ttttgcaaaa ttatgatctt tttttcctaa acgagcgaat tgatattgga
    61561 ttttttctct cagttgagga ttttcctgat aaaaagcaat cagtttttct aactgctgat
    61621 cttcaccaaa atcaaagctg cgtaaacgtt gatacaacgc ttctgaacct acactagaat
    61681 aggtactgtt gattccttcg aatacttcga acatatccag gtcataccaa gtaagatcat
    61741 cgatttcgct atcccatgaa cggaacttct tctccgtgag ccatgcttcc ttcaagcttt
    61801 cttctttatc aaatctcggt tgataaggaa ttttttccca tttgctacga actgtttcct
    61861 ttaatttcaa acgtgaaaaa atgtctaaag caactaataa aataacagct gcaataataa
    61921 ttaaaacgat aaattctctt tgacccatat ccctctccgt tcctttattt gttagcttta
    61981 gtatagcact gttgcgctat ttgaaaagag ggtaaaaaaa cacctttcaa aataaaaggt
    62041 gtttttttct tgtatattac atatttatct ctcatttatc aaaattgaca aataatcata
    62101 gggaggaatt gttgttaatt cttgtttatt ctccagtgca ttgacaactg tcccaaggga
    62161 aaggatagcc tcaggtacta ttttttcttg ttctgttgga ttagctaagc gaagaacaaa
    62221 tccttgttca tgaaaataag aaggataaat cgatgataca attaagtctt caggtaattc
    62281 aatcaatgaa aatttacgag gaacatcaag ttcattttgt aatggccaaa tcttgttatc
    62341 taacctgtga ataaatacat tcagtttctg cttttgataa gaaatagatt gttctaaccg
    62401 tttttctgcc tgttttgcca ctttgaactc attaaatctt ccttcttcta cattaaatcc
    62461 aaaagtgact ttatactcac ctatctcttg agctagcggt gtttccatca tgacatgccc
    62521 ttcatttgtc gtatctcctg aagctcgccc aggtcgccaa gcaagattag gttttccaag
    62581 ttcccctgtt gttgaaaata aggttatgat taattgattt tctatacgtt catactcttt
    62641 taaaccatct gcataaaacg taaaataatg atcattttct gccacagaga cactcttatc
    62701 gaatatttct aaattaacag gtttctctac catagtttct gaccaattcg gaggaatgac
    62761 atcaggggta ttttcaataa atccgttttg aatttgtgca attgattttt cagatttatc
    62821 taaaacatta attgctaagc gcatacgatg cgagtaaaca gtattatgaa ttttgagtgt
    62881 gccttcaacg atttgacttt ctcccagact gaacgatatt tcataagata tttcttccat
    62941 ttcgggtttt tcagataaac gatcttttaa atctttagga agttgagctt ttccgtaaac
    63001 aactaatgtt tcttgtaaag aatctttata tactttagct gtttctaatt ttagagatag
    63061 ttctgtatct ccttctaatg gtgaataatc atacgtatcg ccatcatttg ctgaatcgat
    63121 caaatgaacg aaatcaagat attgtctatc tccaactatc aaattcaatt gcccatcttt
    63181 aaaagaaagt ttatatttat tgttacttat ctcagttttg tctatctcga ttgctgtttc
    63241 taatttttca ttatttttct caaattcaac aacagtgtat cctagtgctg gcacatttac
    63301 atttattttt atgtcaagtt cataataagc tggttcatca aaatattcga aacctgtagc
    63361 aatcatccga taagcatttt tccgttctgg aaaataaact tctttttcaa tgatattata
    63421 gggactacct ttgaagtgta ttttctttga atcagctact acatgcacga ttttcgatcc
    63481 actataattc tcagtcgttg tgttgaaaac aaggacttca ttcgatttta gctttaatgc
    63541 atcagccatc cgttttacaa taaggttttc gattccgtca gctatctcat tagcttcttt
    63601 gattcgatgg aaaatatctt ccgcaacatt atctgaaaca cagccaccaa tactatcatg
    63661 agcctggttt tgcaaaatct ttttccataa ccgacgaagt aggcctttac tgatatggat
    63721 accatttctt tctgcaatca gcatgagcgg ctcaactcgt tttagaattt tttgttccaa
    63781 tttaaaattt tctattttca tttttgttct tactgagcca atagaacgat gaacacgaga
    63841 gtaaaccggc tgacgaagtt cacctttata ttcaggtaat tcttttttgt ttctgataac
    63901 ttctacataa gaaggataat ctgaaataat attgtggaat ttactgtatt tattcaactt
    63961 ctctacgact tgatcaaaat ttaaaatcat tgacttttga tctacacccg aaggtactaa
    64021 aatatctttg ttttttcctt gcatatgaag catttcaact gcttcatcca acttttcttt
    64081 tgcaaattca ggaatatctt taattgcgtc taaggtcatc attccagtca tataaccaaa
    64141 tggaaaattc atggcataaa caaatttatc tcccaatcct ttccagataa aataaggaga
    64201 tggaaccatc gtttcaaagt ttaatcctcg ccaacacatg aaattatcga tttctacttg
    64261 atgcaaaagt gtaggcaaat tcgcattgaa cccaaacgtg tcaggcaaat accctaccat
    64321 cattggatct ccatatttaa ggatcgtatc actgattcca atcattaagt ttcttaaaat
    64381 tgattcagca tccactaata aggcatctgt ttgtgcatac cacggtccca cgaatagttg
    64441 tcctgttttc atcagtcgtt ggataacctt aagcttttgc ggattgatct ccaaatactc
    64501 atcgacgatt gaagtttgtc catctaaaca aaagtttact ttagggttcc tttccaattc
    64561 atctagaact tcagtaaaga cctgatcaga caatacgatg gcatcttcat ttgtaaaata
    64621 ccattctctg tcccagtgcg tgtgttgaat tatatgtgca tttaccatat cagattctcc
    64681 tactttcctt cttctactga atttacttgt tgttcttttt tatacttgat ggattttagt
    64741 gcattgacca ttaacgctgt aaccactccc ccaataacaa ttgatactaa atataaaggt
    64801 atattcgtaa caaaaggaat tgccataaag gttgaaagca ttgtttcttg agtaacacca
    64861 aaaaatccat ttagaccaca tgctactgca gtgcccacca cgatgcttgg tattactcgt
    64921 aaaggatctt tcacggcaaa cgggatcgtc ccttcagtaa tccccacaag gcccataatc
    64981 atagctgtct ttgcattatc tcgttccgat tcatcataaa gttttttgtt taaaattgtt
    65041 gctaaaaaca ttccaaatgg cggaatagca attcctaata acatggctgt actaggtcca
    65101 aagcttccgc ttgaaaaaga agcaacccca aaagcatatg cgattttatt gataggtcct
    65161 cccatatcaa acgccatcat tcctcctaaa atagccgaca tcaaagcagc atttgctcct
    65221 gacatgcctt ctagccaatt agtcagtcct gttgtcattg cttgaatagg cgtagctagt
    65281 acataccata atatgattcc aacaactaat gtagtaacaa aaggaatcag cataattgat
    65341 tttagactaa taacttgcat cggtactttt acttttttca atgcattagc taaatagcca
    65401 caagctaatc cagcaaccat tcctccaata aatcccatac caagattttg acacatcatc
    65461 ccagtgataa aagctggtgc tagaccggga cggtcagcaa ttgaatatgc gatataggct
    65521 gaaatcatcg gtacgataaa ctgtaatccc gtctgaccaa tcattcgcag gactccccag
    65581 aatgtatccg tctggtccca aacacctgtg ccaccaaaaa tgactgcaat agaagttagt
    65641 aaccctgctg ctacgacaag cgggatcata taggaaaccc cactttggaa atgatttaga
    65701 atttctcttc cttgtttctt gaattccatt agtaactccc ccacgatata ttttttattt
    65761 gttaattaat gcaattgctt catcaatcgt ctcttcacca tcttttacta cctctgaggt
    65821 ccctactgtt aaaatcggtt taccgtcaaa gcgacctcgt tctgaaacat ttgtatctac
    65881 tgcaaatatg acaacatctg cctcggaaac atccgaaatt gaaagtttgt tttctatccc
    65941 catagccccc tgtgtttcta ctttaatatc taaccctttc ttcttcgcaa acttttctaa
    66001 attagctttt gccatataag tatgggcaat ccctgcgata catgctgtta ctgctactat
    66061 ttttgtcata tgaaattcct ccttttaaaa tgatagagct tttaccaatt cttttttatt
    66121 tctcgaatct tttattcttt taacaaactc gtcatccatc aatcttccag ataattctgc
    66181 taaaatacgt aaatgattag tacctttatc agtgtctttt attgctaaca aaaagacgag
    66241 atcaactggt tgatcatcta atgcgcccca ttcaacagac cgctttaact tggctacagc
    66301 cactgttgat tcaattactg ctaggctttt gccatgagga atggcgatat ttttcccaat
    66361 tcctgtggtc atatggtttt ctctgtcaaa tacgtctttt tgaaattgtt tgtggtcaga
    66421 cacaaaccca ttagtctcta acaatttcga taatttttcg actacacttt ttttatcaga
    66481 tgcatttaaa tctatcacta tattatttat attgattttt tcactgatca tttataagcc
    66541 ttctttcaat atataaacta atttttcaaa cgacgaactc gtttttaaat ttgctaataa
    66601 ctcatgattc gaagcgattt cattgaatgt ttcataaatt ttattcaatt caggttttac
    66661 tttttcatta agtgcaaaaa agaaaacaga atgaacaagt gtcccatccc aatcaattcc
    66721 ttctggatta acgtagacac agattactgg cttttttata aaatctactt cgatatgcgg
    66781 catagcaaaa tcattcatcg ctgttgaaga tagtttttct cttttgattg ctccctctag
    66841 tatcccttct ttcgcgtaat tttcttttac caattttttg gtaattatat ttaaaacgtt
    66901 cacttgatta tcctttattt ggtgggtaaa aaagcaaaat cgtttatcta ataaatttag
    66961 aaaagcatct tctgtttgtt catttaactg tagatactgt tttacttttc tgacatctgc
    67021 ccctaacatc aaaggactaa caacaataac aggaaaattt gtattttcaa tcggaatcgt
    67081 gcttataaca agttcatcag tgacttttct atcttgtagt tcattgatgc ttagtacatc
    67141 ttctatctca accatatctg caaaaccgtt atttattctt tgctctaata attttgatgt
    67201 tccatatccg ctactacaaa ctaatatcac tttcgttttg tcaggtttgt aacgatccaa
    67261 aagtgattga atatggagtg ttacgtaagc gatttcatct tcattaaaat gtatacagaa
    67321 tctctcttcg atttcctcta ataaatctac acttatcata aatgaccttt taaaagaata
    67381 tttgattttg tccgtataag ggttatagat atttactccc aatttgagtc ttttaactgc
    67441 tgcattgaga tgtaatacta gattattgat caattcttca tcaggataaa attcatttaa
    67501 tttaattctt actaactctt gtagctcaac acccatatct aaataggaaa gcttcgtttg
    67561 atctaagtcg tgattcatag catttttttc aatcgaagta atgtgaatgt caacataagc
    67621 ttgttcatct attggcacaa taattgaaaa tgctttttca atcattttga ttaaaataag
    67681 tgtattcttt gagatttctt cttcagaatt attactacgt gataagaaat tgcttgtttc
    67741 aatcctttct atcgcaatag caaggtggat agctaaagtt tgaaattcat aatctgtaaa
    67801 tttcaattgt gtcttattca tgtatcgatt aagcaattcg gctacttttt tcaatatgtc
    67861 cttatttaag aaattattcg cttcaccaat gaaattgata ttcaagtcta agagatctgt
    67921 tttactgtta gatgctgtaa agccacccca ataatagttc acaacttctg acatcaacct
    67981 tcttttactt ttttcatcag ctttgatgcg cacgccattt cgatctgaaa ctaaggacat
    68041 cccatgcttg tttagtcttt ttctaacatc tttcattgtg ctttctaccg tcgacctgct
    68101 cacgtataat tcgtcagcaa gattttgaat ctttagagaa tcattagtat tcaataattt
    68161 agcaaacaca taagttagac ggtcctccgg agagtctgcg atattgattt tatgactgga
    68221 taataaattt cgaagtgttt ctttttttcc aataagcgaa acgccttttt taggttgaac
    68281 caacatttgt acatctggca ttttttggat tatttctttc aaaattaaaa tatctttgct
    68341 aattgttttc gtagatacat ataattgttc tgataattct ttgtaagttg tgtgctcatt
    68401 ttcaatcaaa aagtatagta ttttcatttg ccgttcgcta aattcgatgc ccattatctt
    68461 cacctcctga atacagctta agtgtacaat tattatctct attttcatac agaagaaagt
    68521 tcttgattaa tgtagaagtt tctaatattc ttgattattt caaatcaata aaaaagcctg
    68581 caatat
//
LOCUS       DAZAID010000003        64864 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000003 DAZAID010000000
VERSION     DAZAID010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.003"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..2330
                     /gene="pcrA"
                     /locus_tag="ACG9BE_000139"
     CDS             96..2330
                     /gene="pcrA"
                     /locus_tag="ACG9BE_000139"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFN3030928.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            2347..4383
                     /gene="ligA"
                     /locus_tag="ACG9BE_000140"
     CDS             2347..4383
                     /gene="ligA"
                     /locus_tag="ACG9BE_000140"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFN3030929.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            4489..4794
                     /gene="gatC"
                     /locus_tag="ACG9BE_000141"
     CDS             4489..4794
                     /gene="gatC"
                     /locus_tag="ACG9BE_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFN3030930.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            4794..6266
                     /gene="gatA"
                     /locus_tag="ACG9BE_000142"
     CDS             4794..6266
                     /gene="gatA"
                     /locus_tag="ACG9BE_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFN3030931.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            6263..7693
                     /gene="gatB"
                     /locus_tag="ACG9BE_000143"
     CDS             6263..7693
                     /gene="gatB"
                     /locus_tag="ACG9BE_000143"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFN3030932.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            7703..8758
                     /locus_tag="ACG9BE_000144"
     CDS             7703..8758
                     /locus_tag="ACG9BE_000144"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFN3030933.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            8869..10245
                     /gene="rlmD"
                     /locus_tag="ACG9BE_000145"
     CDS             8869..10245
                     /gene="rlmD"
                     /locus_tag="ACG9BE_000145"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFN3030934.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            10454..11290
                     /locus_tag="ACG9BE_000146"
     CDS             10454..11290
                     /locus_tag="ACG9BE_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN3030935.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            11439..11660
                     /locus_tag="ACG9BE_000147"
     CDS             11439..11660
                     /locus_tag="ACG9BE_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3030936.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            11661..14378
                     /locus_tag="ACG9BE_000148"
     CDS             11661..14378
                     /locus_tag="ACG9BE_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFN3030937.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            14371..16326
                     /locus_tag="ACG9BE_000149"
     CDS             14371..16326
                     /locus_tag="ACG9BE_000149"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN3030938.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            16316..17452
                     /locus_tag="ACG9BE_000150"
     CDS             16316..17452
                     /locus_tag="ACG9BE_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN3030939.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(17503..17706)
                     /locus_tag="ACG9BE_000151"
     CDS             complement(17503..17706)
                     /locus_tag="ACG9BE_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3030940.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(17722..19554)
                     /locus_tag="ACG9BE_000152"
     CDS             complement(17722..19554)
                     /locus_tag="ACG9BE_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3030941.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            complement(19557..20003)
                     /locus_tag="ACG9BE_000153"
     CDS             complement(19557..20003)
                     /locus_tag="ACG9BE_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFN3030942.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            complement(19996..20946)
                     /locus_tag="ACG9BE_000154"
     CDS             complement(19996..20946)
                     /locus_tag="ACG9BE_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFN3030943.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            21341..21958
                     /locus_tag="ACG9BE_000155"
     CDS             21341..21958
                     /locus_tag="ACG9BE_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3030944.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            22055..22261
                     /locus_tag="ACG9BE_000156"
     CDS             22055..22261
                     /locus_tag="ACG9BE_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3030945.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            22266..22580
                     /locus_tag="ACG9BE_000157"
     CDS             22266..22580
                     /locus_tag="ACG9BE_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HFN3030946.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            22581..23723
                     /locus_tag="ACG9BE_000158"
     CDS             22581..23723
                     /locus_tag="ACG9BE_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFN3030947.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            23725..24267
                     /locus_tag="ACG9BE_000159"
     CDS             23725..24267
                     /locus_tag="ACG9BE_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFN3030948.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            24328..26265
                     /locus_tag="ACG9BE_000160"
     CDS             24328..26265
                     /locus_tag="ACG9BE_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFN3030949.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     ATHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            26476..27246
                     /locus_tag="ACG9BE_000161"
     CDS             26476..27246
                     /locus_tag="ACG9BE_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFN3030950.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            27239..27583
                     /locus_tag="ACG9BE_000162"
     CDS             27239..27583
                     /locus_tag="ACG9BE_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFN3030951.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            27576..29819
                     /locus_tag="ACG9BE_000163"
     CDS             27576..29819
                     /locus_tag="ACG9BE_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFN3030952.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            29957..30166
                     /locus_tag="ACG9BE_000164"
     CDS             29957..30166
                     /locus_tag="ACG9BE_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3030953.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            30163..30447
                     /locus_tag="ACG9BE_000165"
     CDS             30163..30447
                     /locus_tag="ACG9BE_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFN3030954.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            30447..31799
                     /locus_tag="ACG9BE_000166"
     CDS             30447..31799
                     /locus_tag="ACG9BE_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFN3030955.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            31796..32224
                     /locus_tag="ACG9BE_000167"
     CDS             31796..32224
                     /locus_tag="ACG9BE_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFN3030956.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            32392..32757
                     /locus_tag="ACG9BE_000168"
     CDS             32392..32757
                     /locus_tag="ACG9BE_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN3030957.1"
                     /translation="MPMKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAIN
                     KRYGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCK
                     SCHSKIHMTRRNTKDKTIM"
     gene            32891..33463
                     /locus_tag="ACG9BE_000169"
     CDS             32891..33463
                     /locus_tag="ACG9BE_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFN3030958.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            33478..34260
                     /locus_tag="ACG9BE_000170"
     CDS             33478..34260
                     /locus_tag="ACG9BE_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HFN3030959.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            34260..35495
                     /locus_tag="ACG9BE_000171"
     CDS             34260..35495
                     /locus_tag="ACG9BE_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFN3030960.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            35569..35946
                     /locus_tag="ACG9BE_000172"
     CDS             35569..35946
                     /locus_tag="ACG9BE_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HFN3030961.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            35948..36142
                     /locus_tag="ACG9BE_000173"
     CDS             35948..36142
                     /locus_tag="ACG9BE_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3030962.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            36126..36302
                     /locus_tag="ACG9BE_000174"
     CDS             36126..36302
                     /locus_tag="ACG9BE_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HFN3030963.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            36299..36442
                     /locus_tag="ACG9BE_000175"
     CDS             36299..36442
                     /locus_tag="ACG9BE_000175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3030964.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            36525..38090
                     /locus_tag="ACG9BE_000176"
     CDS             36525..38090
                     /locus_tag="ACG9BE_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN3030965.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            38146..38880
                     /locus_tag="ACG9BE_000177"
     CDS             38146..38880
                     /locus_tag="ACG9BE_000177"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3030966.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSLTPDEKS
                     KCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            38946..40274
                     /locus_tag="ACG9BE_000178"
     CDS             38946..40274
                     /locus_tag="ACG9BE_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN3030967.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            40174..40929
                     /locus_tag="ACG9BE_000179"
     CDS             40174..40929
                     /locus_tag="ACG9BE_000179"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFN3030968.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            40933..42144
                     /locus_tag="ACG9BE_000180"
     CDS             40933..42144
                     /locus_tag="ACG9BE_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN3030969.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            42155..42466
                     /locus_tag="ACG9BE_000181"
     CDS             42155..42466
                     /locus_tag="ACG9BE_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN3030970.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            42463..42801
                     /locus_tag="ACG9BE_000182"
     CDS             42463..42801
                     /locus_tag="ACG9BE_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN3030971.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            42794..43225
                     /locus_tag="ACG9BE_000183"
     CDS             42794..43225
                     /locus_tag="ACG9BE_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFN3030972.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            43222..43563
                     /locus_tag="ACG9BE_000184"
     CDS             43222..43563
                     /locus_tag="ACG9BE_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3030973.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            43566..44156
                     /locus_tag="ACG9BE_000185"
     CDS             43566..44156
                     /locus_tag="ACG9BE_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFN3030974.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            44166..44546
                     /locus_tag="ACG9BE_000186"
     CDS             44166..44546
                     /locus_tag="ACG9BE_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3030975.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            45254..47314
                     /locus_tag="ACG9BE_000187"
     CDS             45254..47314
                     /locus_tag="ACG9BE_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN3030976.1"
                     /translation="MGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTV
                     QKNADNAFKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKM
                     GTDIESIQNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFAD
                     VTEAIHVIQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNV
                     VEQFQNVIKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGL
                     LPTLIPVVVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIIT
                     IVNGLIENLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQ
                     TGIQLLTSLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALP
                     QIITTIANAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQII
                     TGLANAFGEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIH
                     SPSTLFRDEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDS
                     TFSMLDLGAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRR
                     RVAY"
     gene            47385..47786
                     /locus_tag="ACG9BE_000188"
     CDS             47385..47786
                     /locus_tag="ACG9BE_000188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3030977.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            47863..48555
                     /locus_tag="ACG9BE_000189"
     CDS             47863..48555
                     /locus_tag="ACG9BE_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN3030978.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            48552..52442
                     /locus_tag="ACG9BE_000190"
     CDS             48552..52442
                     /locus_tag="ACG9BE_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN3030979.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            52452..52619
                     /locus_tag="ACG9BE_000191"
     CDS             52452..52619
                     /locus_tag="ACG9BE_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3030980.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            52606..53013
                     /locus_tag="ACG9BE_000192"
     CDS             52606..53013
                     /locus_tag="ACG9BE_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HFN3030981.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            53014..53433
                     /locus_tag="ACG9BE_000193"
     CDS             53014..53433
                     /locus_tag="ACG9BE_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFN3030982.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            53423..54331
                     /locus_tag="ACG9BE_000194"
     CDS             53423..54331
                     /locus_tag="ACG9BE_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFN3030983.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            54447..55484
                     /locus_tag="ACG9BE_000195"
     CDS             54447..55484
                     /locus_tag="ACG9BE_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFN3030984.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(55545..55850)
                     /locus_tag="ACG9BE_000196"
     CDS             complement(55545..55850)
                     /locus_tag="ACG9BE_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3030985.1"
                     /translation="MSSFDMEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIE
                     AEEALYQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            56145..56318
                     /locus_tag="ACG9BE_000197"
     CDS             56145..56318
                     /locus_tag="ACG9BE_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFN3030986.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            56379..57932
                     /locus_tag="ACG9BE_000198"
     CDS             56379..57932
                     /locus_tag="ACG9BE_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3030987.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            57980..58345
                     /locus_tag="ACG9BE_000199"
     CDS             57980..58345
                     /locus_tag="ACG9BE_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3030988.1"
                     /translation="MDEQQAQQIREFFNIYLEGVGLQEAGKRSGINKPHPFMKRILMN
                     EVYLGNEIYPKIIDEDIFFQADLEQERRIKFFKKRKEIKVPDTKVIPTHFKSGKITEK
                     YSDPFKQAQYAYSKIKEVK"
     gene            58347..59897
                     /locus_tag="ACG9BE_000200"
     CDS             58347..59897
                     /locus_tag="ACG9BE_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3030989.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            60020..64543
                     /locus_tag="ACG9BE_000201"
     CDS             60020..64543
                     /locus_tag="ACG9BE_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFN3030990.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFYKSLLFLPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFD
                     KESMIGDKVAKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDD
                     VPDDSEQINKKIFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMN
                     EFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLV
                     QELANYNEYTGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKD
                     NSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKW
                     EETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFIN
                     WFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTA
                     LKKIDCSSFAEERDFLSFILRCGVSKYPKIYDNDSLKNDVGFKKFIYNKYKNEIKFTN
                     INYLTSKYISNFEELIDKLDTKDIVNWLNQDEELTDLLSSVKKESSASQQSNWSGLYF
                     NSNEYVKYILNKTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYGVSEQSLMELLGQRIV
                     EQLDFRKNMAMFSDDEIRAILVELPKFDKGEISRRLYNELIRNKKGMNPTYSTEGLSV
                     LAKDGFFYPNKELRYADKRLPKSIEKQMRFIYVPEKASTTTIYEWLGVERFKTNLKLE
                     TFELLEDYSEDFKKEIDDIKTAVLSTIDDNSKNVSSLKRIQIIPCSKISAKDVEQDGQ
                     SIVLEDYYFVESKGKFYLKLPANSIEINQLRLIDTFSSTIVDIFKQVLNLSLDLNLIE
                     LLISRDTEHKQRKITEEFGVDKWNESFELLFSENALNKKVNDFLLSNGLNEEEYPTIF
                     EIDYSYALKEEEYQLLAIALESISKDIEDLNGLSELIHLDIREFWKLKLRSYLDKKAE
                     HYRQILYSNANKSGDEILKETFLEKIDRYKDYQFEISEIPNSVQVDIEKESKKRFPEL
                     SVSITDEIDIDGLYDNNVEELVTVAAITIDDFDYYIRGHQKVSSLLYFGIPSRLREDI
                     LIFVTKENDVDLNGEKSGISIEGTSTVQTILEPSNSVSRKGKNTRNGERSKRDYDNRN
                     SANDDAGETAEQIAYAELTQNQKLNVIWNTKYSSKSSDRNKQPPNGIVCDMWVHDPEN
                     GNMYFEVKSSITEFEMSINEYNSMLDNPDSYEVVLVNRDTKEISRHKFDELDGLKQAS
                     SYLFKFKQKRSDG"
     gene            64678..64806
                     /locus_tag="ACG9BE_000202"
     CDS             64678..64806
                     /locus_tag="ACG9BE_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFN3030991.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT        20997 a        11499 c        14615 g        17753 t
ORIGIN      
        1 cataaaaaga aacaaatgat tttgaactag ctagtttaat gaaagccaag aaaatgctat
       61 aattaacaag attgataaaa gaaaggatgt tgttaatgtc atccaaagct gaattattaa
      121 acgggatgaa cccccgtcag aaagaagctg ttcttcacac tgacggacca ttactgctaa
      181 tggcaggtgc aggaagtgga aagacgagag tgctgacaca ccgcattgcc tatttgattg
      241 aggaaaaaga agtcaatcct tggaacatcc tagcgattac ttttaccaat aaggctgcaa
      301 aagaaatgaa agaacgtgtc aatgcgattc tcgcatctgg aggcgaagat gtctgggtat
      361 cgacttttca ctcgatgtgt gtgcgtattt taagaagaga tgttgatttt atcgggtata
      421 accggaactt tacaatcatc gattcttctg aacagctgac cttgatgaaa cggatactaa
      481 aagaactgaa cattgatccg aaaaaatatg atcctcgaag tatattagga acaatctctc
      541 aagcaaaaaa cagcctgcag acccctcagg attttgcaaa aatgcaaggc agttattacg
      601 aagagattgc ggcaaaatgt tatgctgctt accaaaaaga gcttcaatat aatcaatgta
      661 tggactttga cgacctgatc atgaatacga ttcgattatt tgaagaacat cctgattcac
      721 tcacatatta tcaaaacaaa ttccattata ttcatgtaga tgagtaccaa gatacaaatc
      781 acgcacagta tacgctagtc aatttattgg caggtagatt taggaacctg tgcgtcgtgg
      841 gagatgcaga tcaaagtatt tacggctggc gcggtgcaga tatgcagaac atcttggatt
      901 ttgaaaaaga ttatccagat gctgcagtaa ttttgctaga acaaaactat cgctccacga
      961 aaaacatcct tagcgcagca aaccaagtca ttgaaaacaa tagcaatcga aaaccaaaaa
     1021 acttgtggac agaaaacaaa gaaggaaaca agatcactta ctaccgtgca gataatgaac
     1081 gagacgaaac ccgttttatc gtagatcgga tgcaagaaga gattcgaagc aatcatcgga
     1141 attacggcga ctttgccatc ttgtaccgga cgaacgcaca atctcgtgta atggaagaaa
     1201 ctctgttgaa agcaaatatt ccttataaaa tggttggagg gcacaagttc tacgatcgta
     1261 aagaaatcaa agatatttta gcgtatttga atgtgctggc aaatcctcaa gattcaatca
     1321 gttttgagcg tatcgttaat tcgcccaaac gaggcatcgg accaggatct atcgaaaaac
     1381 tacgcagttt tgcttctctt catgaatggc cactgcttga agcagcgcaa aatgtggatc
     1441 tggcaaatat cggtggaaaa gcaggacagc aattaggtgc atttggagag atgatccaag
     1501 aagtcactca gatgatccca tacttgacag tgacagaatt gacgaaagag gttttggatc
     1561 gtagcggata tctagaagac ttaaaaatcc aaaatacatt agaagcacaa gcacgcatcg
     1621 aaaacttgga ggaattttta acggtcacac aagaattcga caaacaattc gaacagcaaa
     1681 atgaagaaga cgctgatgca ccagaagaaa aactaacggt cttcttaaat gatctggcac
     1741 ttgtttccga tattgacaat ttagaagaag atgcttccca agtgacattg atgactttgc
     1801 atgcagccaa aggattagaa ttcccagttg tattcctgat tggtctagaa gagggggttt
     1861 ttcctttatc tcgagcattg atggaagaaa gcgaactgga agaagaacgc cgtctcgcat
     1921 atgtggggat cacgcgagca gaagaagcgt tgtatttaac aaatgctttc tccagaacat
     1981 tatatggacg gacgcaatat aaccgacctt ctcgttttgt agaagaaatc gatcaggagt
     2041 tactggaaat cgaaggcatg cgtcctacgc ctaaaaaaac acctgtattc gctaaaaaaa
     2101 cagcatatag ctataaacag ccagagacag cggttgttcc ctctaaaagt gctactggag
     2161 gggaaaataa cagctggaaa cctggagata aagtcaaaca caaaaaatgg ggtctaggaa
     2221 cagtcgtccg agtaagcggg acgtcgaagg atctagaact agatgtggct ttcccaagtc
     2281 aaggagtaaa acgtcttttg gctgcctttg cgccaattga aaaagcttag tagtggaggg
     2341 taaatgatgg aacatgaaat gacgattgac gaagctgcta aacgagcaga agaacttcgt
     2401 acaagactga accaatggtc tcgcgaatac tacgtagaag ataaaccaac agtggaagac
     2461 tatgtttatg ataaagaata cgcagaatta gtagcaattg aggaacaata tcctgatttg
     2521 atcacgtctg attctcccac tcaacgagta ggcggaaaag tattagaagg ttttgaaaaa
     2581 gtcactcatg atattccgct ttacagtttg aacgacgttt tcagcaaaga agagctgata
     2641 gcttttgatc aacgtgtaca aaaagcagtg ggacgtgtag ttgactattg ctgtgaacta
     2701 aaaatagatg gactttccgt ttctttgcgt tatgaagatg gaaatttcgt gagaggtgcg
     2761 actcgcgggg atggaactgt aggagaaaac atcactgaaa acctaaaaac tgtccggtcg
     2821 gtcccaatca aattaaaaga accaatgaac atcgaagtaa gaggcgaatg tttcatgcca
     2881 aaacggtcat ttgtccaatt gaaccaagac agagaagcag agggaaaaga tatctttgcg
     2941 aatcctcgga acgccgcagc tggttctttg cgtcagttag attctaaaat cacagcgaaa
     3001 agaaatctgg acactttttt atacacagta gctgatttcg gtcctatgca agcaaaaacc
     3061 cagtatgatg cgctagaaga gctagaaaaa attggattcc acacgaatcg ggaaaaacgt
     3121 ctatgtcatt cgattgatga agtgtggtca tacattgaag aatatcatga caaacgtgtg
     3181 gatttgccct atgaaatcga tggaatcgtc atcaaagtga atgaattctc gctacaagat
     3241 caacttggtt ttactgtcaa agcaccgaga tgggcagcag cttacaagtt tccgccagaa
     3301 gaagttgaaa cgctaatcga aaacatcgaa tggacagtcg gtcgaacagg ggtagtaacg
     3361 ccaacagcta tcatgacacc agtccgagta gcaggaacga cagtaagccg tgccagcctg
     3421 cataacggtg attatattaa gttgaaggat atccgcttga aagatacagt tctgatttat
     3481 aaagcgggag acatcatccc agaagtttcg caagtcgtgt tagataaacg acctaaagac
     3541 agcgaagaat accaattgcc tacacattgt ccagtttgtg gaagtgaact tgtccatttg
     3601 gatgaagaag tagccttacg gtgcatcaat cctaaatgcc ctgctcaaat gaaagaaggt
     3661 ttgaaccact ttgtttcaag aaatgcgatg aatatcgatg ggttggggcc gcgcgtacta
     3721 gaacagatgt atgataaaaa actagtagca gatgttgctg atctttacaa attgacagaa
     3781 gaagagttat tgacgttgga caaaataaaa gaaaaatcag caaataatat tttgacagca
     3841 atcgacaaca gcaaagataa ttcggtcgag cggttgattt tcgggttagg aatccgacat
     3901 gtcggagcga aagcagcaaa aattttagca gaacatttcg gtgatctaga aacattgagt
     3961 aaaagtgact atgagtcgat tattgcgctt gatacgatcg gtgatatcat tgccgacagt
     4021 gtagtcactt atttcagtaa cgaagaagtc catgaattga tgaatgaatt aaaacaagca
     4081 ggcgtcaatt tcgaatacaa agggcttcga aatgcccaat tgcaagaagt cgagtctcct
     4141 tttaaagaaa aaacagtagt ccttactggt aaactaacac gatttactcg tgaagaagca
     4201 aaagagacga tcgaaaatct tggcgggaaa gtgactggta gcgtatcgaa aaaaacggat
     4261 atcgtggtag caggagaaga tgctggcagc aagctgacca aagcacaaga attaggaatc
     4321 gaagtttgga cagaagatca aatggcagat gcattggcta aaagtcgctc agtagaagaa
     4381 tgaaaaatga gttttttcag acaaatttca ataaacaatt tcagacacgg cactcttgtg
     4441 ttaaaataga gagcaaagtt tttaactcgt ttagaaagaa ggggtagtat ggcgattagt
     4501 gaagaacaag tgaaacacgt tgccaaatta gcaaaacttg cgttcgcaga tgaagaactt
     4561 gcaagtttta ccgaccaatt aggtaaaatc atcgatatgg ttgaaatgct ggaagaagta
     4621 gatacagaag gtgttccatt tacgtcgaat gtggttgaaa cagtaaacgt gatgcgacaa
     4681 gatcgggcgc aagctggatg gaaccgcgat gaattattga aaaatgtgcc ggaaacagaa
     4741 gacggcttta tcaaagtacc cgcaatcatc gataatgggg aggctggcgc ataatgacag
     4801 aattacacaa tcgttctcta gaagaactgc atcatatgct tgtatcgaaa gaaatcactg
     4861 tacaagattt gacaaaagca acatttgaac gaatcaaaga aacagagcct gaggtcgaag
     4921 cattcattac attgaacgaa gaaaaagcat tggaacaggc aaaagcttta gatgaaaaag
     4981 ggattgatga aagcaacgtc cttgctggta ttccaattgg tatcaaggat aatatcgtaa
     5041 caaaagatat cttgacaacc gctgcatcta agatccttta caattttgaa ccaatttatg
     5101 atgcaacggt catggataaa atctatcaag cagatatgat ccctgttggt aagctaaaca
     5161 tggatgaatt tgctatgggt ggaagtacag aaacttctta tttcaagaaa acaaaaaatg
     5221 cttgggaccc aacaaaagta cctggtggct cttctggtgg ttctgcagca gcagttgcag
     5281 ctggacaaat tccagtttct ttaggtagtg atactggggg aagtatccgc caaccagcag
     5341 cttttaacgg catcgtaggg atgaaaccta cttatggccg tgtctcacgt tttggtttga
     5401 ttgcctttgc ttcatctctt gaccaaatcg gaccaatgac ccgtacggtg aaagataatg
     5461 cattagcatt gacagctatc agcggttatg acgaaaaaga cggtacttct tcaggcgttt
     5521 cagttcctga ttttgcagag ggactgacag gcgacatcaa aggaatgaag attgctttac
     5581 ctaaagaata cttaggcgaa ggtgtggccc ctggcgtaaa agaagctgta ttgaaagcag
     5641 cagaaacatt ccgttcatta ggtgctgttg tggaagaagt cagcttgcct cattcaaaat
     5701 acggtgtggc tgtttattat atcattgcct catcagaagc aagttccaac ttgcaacgat
     5761 tcgatggtat ccgctacggt tatcgctcag aaaatgtgaa atcattagat gatgtatatg
     5821 tcaactcacg ttcagaagga ttcggtgaag aagtaaaacg ccgtatcatg ttaggtacat
     5881 tctccttaag tgctggttat tatgacgctt acttcaaaaa agcgggacaa gtccgtacat
     5941 tgatcaaacg agattttgaa aaagtctttg cagattatga cttgatcatc gggccatctt
     6001 caccaacagt tgcattcgga atcggtgaga acattaacga tccaatcaca atgtacatga
     6061 gtgacatctt aacgatccct gtcaacttgg ctggacttcc aggtatgtct gtaccggcag
     6121 gtttatcaga aggattacca gttggattgc agatcatcgg taattatttt gatgaaaaaa
     6181 cgatgtatca agcggcatat gcgtttgaac aagcaacaga attccataaa caacaaccag
     6241 ctattttagg agggaaagac gaatgaattt tgaaacagtc attggactag aagtccacgt
     6301 cgagttaaaa accaactcaa aaattttctc tccagcgcca gctcattttg gtgcagaacc
     6361 aaacagcaat acaaacgtga tcgactgggg ttatccaggc gtgctgccag taatgaataa
     6421 agctgcgctg gaattcggga tgaaagcagc tttggcatta aactgtgaaa tctctaaaga
     6481 tactcatttt gaccggaaaa attactttta cccagataat ccaaaagcat atcaaatttc
     6541 ccaatttgat caaccaatcg gtcatgacgg atggatcgaa atcgaagtag aaggtaaaaa
     6601 gaaaaaaatc cggatcgaac gtgtccattt agaagaagat gctggaaaaa acatgcatgg
     6661 tattggcggt tattcttacg ttgatttgaa ccgacaaggc acgcctttga tcgaaatcgt
     6721 ttctgaagct gatatgcgtt caccagaaga agcatacgct tatctagaag cattgcgatc
     6781 aattatccaa tttacagaag tcagcgatgt gaaaatggaa gaaggctcta tgcgctgtga
     6841 tgccaatatt tctcttcgcc cttatggaca agaagagttc ggtacgaaag cagaattgaa
     6901 aaacttgaac tctttatctt tcgtgaaaaa aggtttagct tttgaagaaa aacgtcaagc
     6961 aaaagttctt ctttcaggag gagaaatcca acaagaaaca cgccgttttg acgaaacaac
     7021 gaataaaaca gttttgatgc gtgtcaaaga agggtctagt gattatcgtt acttcccaga
     7081 gcctgatatc ccgaaattcg tgatcgatga cgaatggatt caaaaagtaa aagctagttt
     7141 gcctgagatg ccagcttctc gtcgcgaacg ctatatccgt gagtttggac ttccagagta
     7201 tgacgcaatg gtattgacac ttacaaaaga aatgtctgat ttttttgaag ctgttttgga
     7261 aaatggtgca gatgccaagc aagcatctaa ctggttgatg ggtgaagttt ctgcttactt
     7321 gaacagtgag aaactggaat tagctgagac aaaattgaca cctgagaact tagcaggaat
     7381 gattcgtttg atcgccgatg gaacgatcag ctcaaaaatc gccaaaaaag tcttccgtga
     7441 attgatcaca aatggcggag aagcccaaga agtagttgaa aaaaatggtt gggtgcaatt
     7501 atctgatccg aacaaactac tgccaatcat taatgaaatt ttggataaca accaacaatc
     7561 tgtcgatgat ttcaaaaatg gaaaagatcg tgcggttggc ttcctagtcg gtcaaatcat
     7621 gaaagcaact aaaggacaag caaatcctgg tgtcgtgaat aagctactta aagatgaatt
     7681 agcgaaacga tagaggctga aaatgaaaaa agcacgcgta atttataacc ccacctcggg
     7741 aaaagaatta ttgaaaaaaa atttggcgga tatcctacag gcattagaag aagcaggatt
     7801 tgaagccagc gcctatgcaa ctacaccaga accagattca gccaagaatg aagcatgtag
     7861 agtcgcagaa ctaggttttg atctagtagt ggcagctggt ggcgatggca caatcaacga
     7921 agtggttaat ggaatcgctc cgcttgaaca ccgtcctaaa atggccatca tcccagctgg
     7981 tacaacgaac gactatgctc gagcattaaa aattcctaga gataatatta aagcagcagc
     8041 agaagtgata aaaaaacacc aaaccgtaaa aatggacatc ggcctttctg ataacagtta
     8101 cttcatcaat atcgctgcag gtggctattt gacagaattg acttatgaag ttccatcaga
     8161 actaaagagt atttttggtt atcttgctta tcttgcgaaa ggagcagaga tgctccctcg
     8221 agtaaagcca atcaaaatgc gcttgaagta tgatgaaggg gaatatatcg gaaatgcttc
     8281 gatgtttttc ctagggctga cgaactctgt tggaggattc gaacagatcg cgccagatgc
     8341 caaattggat gatgggaagt tttctttaat cattgtgaag acagcaaata tctttgagat
     8401 cttgcatatt gcagcactca tgctgaatgg cggtcgacat gtcgatgatc caagaataat
     8461 ctataccaaa acaagcagtt tgttcgttga aacagaagat gatatcaatc gaccagtaat
     8521 gatcaactta gatggtgaat acggcgggga agcaccgatg catttccaaa atcttcatca
     8581 acacattgag ttctttgcca ataccgatca gattcctgat gaagcaatca caggtacaga
     8641 tgaagaagaa ttagaggttg tcagcaaaga attcgtaaaa gaagtcgaac gtttaacgga
     8701 tgaagatatc gacggtgacg gaaaaatagc agacgaaaca cctaatcaaa agaattaatc
     8761 aaaagccggt gctgaacgta gtaaggtcag tgccggtttt agttgtgtaa ttagctgatt
     8821 tatagtagag ttcagtagga ttgaaaaata ggcttaaagg agcaaaatat gacagaacag
     8881 caaatagtaa agaaaaacga acgatataca gtcgaaatcg tcgacttgag ttatgaagga
     8941 atgggggtag caaaaatcga tggttatcca ttatttatcg aaaatgcact ccctggagaa
     9001 gaagtagaaa tactagtagt aaaagcaggc aataagtttg gatatggaaa agtcgaaaca
     9061 tttattacac aatcacctga tcgtcagcct gtagaaaaca atgcattact tcgcataggt
     9121 atcgcacctt tagcccattt gaagtatgaa caacagcttg tcttcaaaca aaaacaagta
     9181 gagcatgtaa tggcgaagat tgctaagatg ccagaagttc ctgtacttcc aacggtcgga
     9241 atggcagaac cattcggcta ccgtaataaa gcacagatcc ctgttcgccg tatcgatgga
     9301 cagttagcca ccggttttta caaaaagaat agccatgatc tagtccctat cgaagatttt
     9361 tatatccaag atcctgtcat cgatcaagca atcaaagtag tcagagatat cttgcaacgg
     9421 ttccaagtac gaggatataa tgaagaaaaa aatgaaggac agcttcgtca catcattatc
     9481 cgtcgtggtc attattccca tgaaatgatg gtcgttttag ttactcgtaa agaaaaattc
     9541 tttaaatcca aggaaattgc tgaagctatt catgaagaat tgccagaagt ggtttcggtc
     9601 atccaaaata tcaatgaaga aaaaacaaat gtgattttag gagacaaaga aaaagtttta
     9661 tatggcagag actatatcga agatcagcta ttaggaaaaa cctatcgaat ctcagcaaag
     9721 tcattttatc aagtaaatac tcaacaagca gagtatctgt accaaacagc catcgagttt
     9781 gccgagttag ataaaaaaga cattgtgatc gacgcttatt caggaatagg tacgattggc
     9841 ttgtctttag cagacagagt ggataaggtg tatgggatgg aagttattcc agaagcaatt
     9901 gaagatgcac agttcaatgc tttaaataat aaaatcgaaa atgttcatta tgaagtcgga
     9961 aaagcagaaa aagttatgaa aaaatggcaa gaaaaaggga tcaaaccatc tgtgatcgta
    10021 gttgaccctc cacgaaaagg attagatcat cattttattg aatcagcaat cgaaatgtca
    10081 ccagaacgaa tcgtctatat ctcatgtaat ccggcaactt ttgcaagaga tgctaagtta
    10141 tttgcagaag caggttatgc cgtgaagaaa gttcagcctg tagatctttt cccgcaaacc
    10201 catcacatcg agtgtgtagg actgcttaca cgtgtggaac gttgatatga caaggattag
    10261 agcgattcgg gatggtttcc ggattgctct tttttcgtgt tgaaatgacg atttttgggt
    10321 gagcgtagag ttgatggaaa tgctatggag ctttgagttg ataggagtta caccgtagac
    10381 ttgataggtt agatgatttt gataacggtt acgtgatata atatattatc gagttttcaa
    10441 gggagacaaa cttatgaagc aaatttttat attttcagat acacagaaag ataatatttc
    10501 ttattttgaa cgcgagccag caatcttggt ttcaatgagt cgtagtgatt tgccgtattt
    10561 gaaggaacag gatttttcaa attatccagc tgtttatgtt ttgattggtg gtaataagcg
    10621 ttacgtgggt caggcagcag ggcaatcaat ttcacttcgt ttatctcaac actttctcaa
    10681 ggaatataaa gcttgggttg aatcagtttt attctttaca agagcagatg ggaaaatgtc
    10741 aaaagcagat acagattatc ttgaaaaacg acttatccaa gatttccaag aaaaatctga
    10801 ctatgaaatg atgaattcaa ctgctggtaa tacttcgtat attgataaac ttcaaaaagc
    10861 aaaatcggat cagctatatg acacagtgtt tgaaattata gatgaaattg caaatatcga
    10921 tttgtttggt acttccgaag acagtgaaac ccaaatttat gaatttagtt catctggaca
    10981 atatgaaatt gagtatgatg ggaaaaagat atcttcgaaa tctgctagag gtttattggt
    11041 tgattttgtt tctcagctat ttgcggatgg aaactacaac aatcaactgc aagaattaat
    11101 tgtcgatgag acgccgtcgt tcgctataat tttaggacgt aaaccatcca tgtataacgg
    11161 aagaccaaat tcagccaggg tagctgatgg agtatgggtt tatgcaaatt tcaacaaaaa
    11221 gaatgtcaga atcaaaattg ataaactagc tcgtcaattg gggattccgg ttaaaataaa
    11281 gtggaattaa tatttgttat ctcgccttta gtaaatctat gctgaaggct tttattttag
    11341 aaaaatagtt tgcgttcgcg aaaatatttc atgtttcgta taataatgat agctcataga
    11401 tgatataatt gaagatacca gtagaataga ggtgtagtat gtcaatttct tataataaat
    11461 tgtggaagtt attgattgat ttgaatatga acaaaactca acttcgtgaa atatctggcg
    11521 tcagtcccaa tgtaatagca aaactcggta aaaatgagtt ggttgcaatg gagtcattat
    11581 tgaaaattgc tgctgcctta aatgttgatg ttagtgaaat aattagttca gatgttatag
    11641 atgcggagga tgttaaataa atggcaaaaa ataaagtttt aaacatcact gaaattgaag
    11701 atggtgtaaa aaaaatactt tcaaataggg gcgatgattt catcactcaa tttttgtcct
    11761 tgtacgatat ccccaaaacg tcgattacac gtgcaaaatc aaaattcgat aatggtgaac
    11821 catttgtgat taagaacaaa cttcattatg aagaaattga aggcaacgtt gtcattgcga
    11881 ttgatgcgat tgctcaatca atcaaagagc aaaaatctaa accacgatat atcttggtaa
    11941 acgattattt agatattgct gcaattgata cgaaaactca tgaaactttg aatattccaa
    12001 ttgaagagct accggctaat gctgactttt tcttagcgtg gaatggcatt gagaaggctg
    12061 attatcaagc tgaaaatcca gctgaccgaa aagctgctga acgttttgca aaattgtatg
    12121 atgtggtggc taaagataat cctgatgcca acgagcatgc atttaacttg ttcttgattc
    12181 gagtgctgtt cttgttgttt gcagaagata caggaattat gaataagggt tcctttacaa
    12241 atgttctaaa aacaagaacg gctgaagatg gttcgaattt taacgaagta atcaaagaac
    12301 tctttgcaat cttggatatt aatgagttag ctcgcaatgg caaggatgag tggctacttg
    12361 aatttccata tgttaatgga aaactgtttg gtgagccaca tgtggatttg accttttcaa
    12421 aagtttcaag agagctgctt attgaagcag gggaattatt gaattggaat gaaatcaatc
    12481 ctgatatcct tggcgcaatg attcaatcag ttgcttcagc agaagaccgt cacgttgccg
    12541 gtatgcatta tacaagcgtg ccgaatatca tgaaagtcat caagccgtta ttcttagatg
    12601 atttgacaga gtcatttgaa actttaaagc ttcgcagtga agaaaacgag ctgaaggaca
    12661 ttactgaaaa aacaagaaac gataataaga gaacaatcat caatgatcta agtgctttgt
    12721 tgacgcgaat ttctaatatt aagtttttgg atcccgccag tggttctggt aattttctga
    12781 tcattgctta taaggaaatc cgtagacttg aaattaagat tttgcaattg ttgggcgact
    12841 tacaaaataa tgattccatg ccactatcag tgattcatct tggtaatttt aatgggattg
    12901 aattggatga ttttgcgcat gaagtagcaa gactttcatt atggattgct gaacatcaaa
    12961 tgaataaaga gcttgaagaa gcaataccag gtaccattgc tgagttgtta ccgttaaaag
    13021 atgctgggaa tatagtgtgt gccaattctc ttcgaattga ttggaatgag gtgttgcgac
    13081 tcaaagaaaa tcatgaaata tatataatgg gaaatccacc gtatctgggc aaaagtcttc
    13141 aaaatgctga acaacgtgaa gatatggaaa aacttgttgg cgatattcga aaatataaat
    13201 tgcttgatta tatttttggg tggttttgga aagctaaaca atatattgca acatctcatg
    13261 gctcgttcgc atttgttact actaattcaa tttgtcaagg tgaacaagtt agtatccttt
    13321 ggccgtacct tttaaaagat gaggtaagaa tttcatttgc atatcagagc tttaagtgga
    13381 acaacaatgc aaagaataat gccggtgtga ctgttgtcat tattggtatg acaaataatg
    13441 tgaatattca tcctaagtta tatttagaga atgggtcagt aatgagtgtt tcaaatatta
    13501 atgcatattt agctaatggt gaaaatgtat ttgtcaacga atcaagaaat agtatttcaa
    13561 atttaccctc tttgaaattt ggcagtatgg caaatgatgg tggtaatcta gttttttcag
    13621 tggcagaata ctggaatttg attcaaaaat atccagatat taaacattta tttaagaaat
    13681 atttaggatc aacaggatta ataaatggag acttaagata tactttatgg ttgaccgagg
    13741 aagatgcaac tagatacaaa caaaattctc ttattaaaga aacaataaaa aatgtaaaaa
    13801 gttataggga aaaaagcaag agaaaagaaa ctcgagcact agctgagaag ccatggtcat
    13861 ttggtgaagt tcgttatgat gaaaccagtg agaatagtat tctaattcct cgtgtgtcat
    13921 cagaaaaaag agattatgtt cctatgggta tagttgggag cgatactata atttcagatt
    13981 cggctatggc aatctataac gctccaatgt ggctgcttgg tctgttagaa tcccgaatgc
    14041 atatggtgtg gctgcgctct gtcggtggga aattagaaac tcggtaccgt tattcagccg
    14101 ggttagtcta taatactttt ccgattcagg atttatccgc ccagcgtaaa aatgaaatga
    14161 ctcgcgtgat gacagaaatt cttgatttac gagaatacga aggcggcacg ttagccgatt
    14221 tgtataataa agacagtatg ccagaatcat tacgtaagaa acaccaggaa ctggatggga
    14281 ttgttgatag agcttatcag caacgcccgt ttgaatctga cgaagatcga cttggaacat
    14341 tactaaaact ctatcaggag atgacaatca atgactagta atttatttga aattaatttt
    14401 gataaaaatc aatcaaaaac aacaaatgac ctcggtatgc gtgaaatgca agaacgtgtt
    14461 tatgaaaagc gtgcgtcaca gtatttgttg gtaaaatcac caccagcttc aggaaaatca
    14521 cgtgcattga tgtttgttgg cttggataaa ctccataatc aaggaattaa aaaagtggtt
    14581 attgcagttc ctgaacgctc aattggtaaa tcatttaaag atactgaatt atcgaagttt
    14641 ggtttttatt gggattggga agtaaagcca agtgacaact taactattgg gggcggtgaa
    14701 aaatcaaagg ttcaacactt tgttaaattc atgaactcga ctgacccaag tgataatatc
    14761 ctgattgcca cacatgctac gttgcgattt gcttttgaag agttggacga ttcattattt
    14821 gacaatacgc tattagcgat tgatgagttc catcatgtga gccgtgatga taaatctgtg
    14881 ttaggtaatg ccttacgtag catcatgtca aactctaatg cgcatatctt agcaatgact
    14941 ggttcatatt tccgtggcga ctctgttcag attctggaac cagccgacga agataaattt
    15001 gataaggtca cttacactta ttatgagcaa ctggaaggat ataaatatct gaaatccttt
    15061 gcaatggggt atagtttcta tcgtggaaaa tatacagatg ctttggataa agtaattgat
    15121 gtcaacaaaa aatcaattat ccatattcct aatgttaatt ctggtgagtc tactaaagat
    15181 aaatacgcgg aagttgatga aatacttgat ttattgacgg acggtggtaa tatcacacag
    15241 aacgaggatg gtatttatga aatcgaacgt ccagatggta aaaaactatt agtggccgac
    15301 cttgtgaatg aagaaggacg tgaaaaagtc tcagcatatc tagcaagtat tacggatgac
    15361 attaaagatc ttgataagct tgatatcatc attgcacttg gaatggcaaa agaaggcttc
    15421 gattggccat ttgcggaata tgcactgaca atcggttatc gtaactcatt aactgaagtg
    15481 attcaaatca ttggtcgcgt aactcgtgac agccccaata agactcatgc acagtttaca
    15541 aatcttattg cgcagccaga tgcacaggat gacgaagtat tattcgcggt taacagtatt
    15601 atgaaggcaa tttctgcatc tctacttatg gaacaagtgt tggcgccagt ttacaagttt
    15661 aaaccaaaag atggtaagcc agacaaacca ggtgatcttt caatcagagg actgaaacct
    15721 gctgatacga cggaacgtac aaagaaaatc atcgaaaatg atatggaaga tttgaaatct
    15781 agcattttac agagccaaga tattcaaaat gcgattgttt caggtgctga tgcgaagatg
    15841 attaataaga cgttgattcc acgtgtcatc attgatcgtt atcctgattt gacgaacgaa
    15901 gagttagaaa ccgttcgtca acatacagtt gccgatataa atattgcggc tggacggaca
    15961 acaacttcag aagatggtag ccgcgaaatt attaagatgg ccgatcgttt cattaacatt
    16021 gatgaattga atattgattt aatcgatcaa gtgaatccgt tccaacgtgc gtatgaagtg
    16081 attagtcgtg atattgatgc accgactctt cggtttattc aggattattt gtcatcaaat
    16141 aagtatgatt ttactgacga acaattggtt gcggcctacc aagctgctaa gaagttccgt
    16201 gcagaaaatg gtcgaaatcc tgatcgaaat agtaaagatg atggtgaaaa atacttggcc
    16261 tttgctttgc tacgacttgc tgagataaaa cgtgaaagag aggcagcgaa agaagatggc
    16321 gaatagttta gatagcatct ttgaagatga cgcttttgat gatttaacca agcaagttga
    16381 aaaaaagaaa gttgtaaaac aagacccaga agttgctaaa tttcaggaga ttatagactt
    16441 tgtgaatgaa aacggacgag agccagaaaa aactcctgaa tggtctagtg aacgcgcatt
    16501 gtgggctcgt cttagcggat ttcgaggaaa acccgaaagg tctgagaagg ttaagaagta
    16561 tgacactctt ggtttattgg agaaagaatt cgaaccggat ggagagtttc tcgctgattc
    16621 agttgaaatc gatgacgagt tgaatttaga tgatatctta ggctcagatg atatgattga
    16681 tgatttttca gacttgttgg atgtttcccg ctatcgtaga actgtgaacg ctgcagataa
    16741 aattggccgc agaaaacatg caagtcattt tgaaaagtat aagagcttgt tcgatcaagt
    16801 gcatgcagat atcgcatctg gcagacgaaa aattatcccg tttgagcagt atgatattca
    16861 tgaaaataga ttctacatcc aaaacggcgt aatgatgtat atcgtttcca tttcgaagga
    16921 tacctttatc gctgataatg ggaaaacaaa tgccagaatg catgttgttt atgagaatgg
    16981 gacagagaat aagaatctgt tgttacaatc tttagcttcg tctctatact cgactgaacg
    17041 tcatggaagg atggtgacag agccggtaga tgaggaaaca ctggctgaaa gctttggaga
    17101 agagtttacg acaggttata tctacgtgct gaagtcatta agcacgaatc cggatattag
    17161 caagtggaat aacctgtata agattggttt cactcgaaat gatgttgaaa gtagaattgc
    17221 caatgcggaa aacgacgtga cctacttaaa tgcaccagtg aaggtcatgc tttctgctga
    17281 agtacaaaat gtcaacgctc agctacttga gcgcactctc catcatgtat tccaggacaa
    17341 acaagttgtc tttcaggatg gaaacttcaa aaaagctact gaatggtata tggttccatt
    17401 gaatgatatt gaatcgaaaa tcaatgaagt aatcgctgga ttacaaaaat aaatatgaaa
    17461 atgcactgta ctcatgaatc gaatacagtg catttttgat atttaatcgc gaacgatttc
    17521 aacaatatcc gataaatcac aatcaagtgc ttcacagatg cgaagtaaga tatctgttgt
    17581 tacattttcg cctttattta gttttgagag tgttgctgaa ctgatatcag tcatttttct
    17641 gagttgtgct ttattcagtc ctctgtcgat taggagtttc caaagcggat tgtagctaaa
    17701 cttcatgtaa tcattcctgt tctagttata aatatcgtat agatatccat agcgtccatt
    17761 gtttttatct tcagtaaaga caatctgatt tacttgttta tcatcatagt ttagtgctgg
    17821 caagttcgtt gtattctcaa cgatgataag ttgtccttcg ttttgatgat ccataaagta
    17881 tttgaaaagt ccttctttca tattgtcatc gccatcttgt tttttgcgtt catctaatcc
    17941 aagaagaggg gaatcgataa tgaacacaga aggtgtgtac ttagcgtgat tatacaagag
    18001 atgtcgatat gccattacca ctactgaatt gataaacgct gtatatccat gtccgttttc
    18061 ggtccatttt agatttccat tgattttgat gtcgaaaata tccttggcga aaaatgcgtt
    18121 acgaaattca ttaggtttat aatgcatatt acgtaacatg gagtccagta actcagacat
    18181 ttctttgtag aattctgcat caaatttctc tttaggttta taaaggatac gttcgtcttc
    18241 tggctcattc tcgatttttt caagatcact tttaagctta ttttgcattt tttccatcga
    18301 gattagctgt tgtttaatat ccctgtaagt ttcgatttta ttgattcgat cctttaacgt
    18361 ctcagctttc ggttttagtt catcgttgat tgctgcatca agcgatttct ttcttgtttt
    18421 aagctcatca agctgttcca ttaactcttg cagttcaact tcggtatcat gagattcttc
    18481 cgtcaaatct gataattgag agagcaatga atgtaattct gacctagcag tatcgatgta
    18541 ggttgtttgg acaggtttac ttagttcgcc gttacagaat ggacagctat gtgaagtatc
    18601 atgttcatta tgatcattta ataatcgctc accatctacg attagtgtta gtcgttcaat
    18661 atccgattga tattgataat gtaactgttt gtacctattc aaaagaagtt gagtttcatg
    18721 aagatactgt tccttttgat aaatctcttt tatcaagatt ttgctgctgg ctaacgcatc
    18781 attgatttgc ttttcaattg tttcaagttc agcgaacatt ccatcgacaa tttgttgaaa
    18841 atcatcagag ggagcctcag gtaattctga tttagccact tcgtagtttt catataattc
    18901 agaaaggctt gaattgatgt agttaatcac cgagttcctt ctaatttttt tatgttcttt
    18961 tgtttcgttt tcttccgagt tcgcaaaatc tgaaccgtat agcagaagta acaacgatga
    19021 taaaaaagct gtgcgttggg ttgtttcccc aggaaaaaga acagaggcgc tggtattgac
    19081 tctatcttgt ggaataagtg tgctaggcgc taatgtccgc caagttaatt tttgacgttt
    19141 gaaatcctca ttcttgataa tctgataatc atcatcgatg cctaaaagtt gtaaccaaaa
    19201 atggctaatt ggatttttcc cagatcgtgc attatatttt tcataatcta catattcacc
    19261 tgaactcgtt attttggata ccaaaatgga gttgctatca aattctctag ttaatgaata
    19321 tttgccatta gtcgattgaa taatcagttc aactttactg tatccggttg catcggttga
    19381 aaagggagca gcgtcttttt tcgcgccata gagaaaatcg attgccgtaa caatgctagt
    19441 ttttccggta tttgaagggc cagaaattat gtttagtcca ttggtgaact tgacagttgc
    19501 atcttcacga tctttgcctt ttatggtaac acgttcaata taataatttt gcattatcat
    19561 tgctctcctt taaattcctt tgcgcgttgg tttatttgtc gcagaagctc aacgtcactt
    19621 ttatcttgaa tgtatttaac agattcgtca gctatttgta agtatttttc tgcatatacc
    19681 gatacgaatt gtgatatcaa ctttaggcca tcttctgata aagcatactt aaaaccttct
    19741 ttgctttgcg atggttggat aacatgttgc agtacaagat ttttgactgc atctttaact
    19801 aattcacgtc gcgcaataaa ttcactgaat ctatagctgt tatcaccatt taagttgtaa
    19861 tcggaaattt caaagtcgcg accatacgtg gtgatgaagt ctgtgattaa aattctatca
    19921 gcgctgacag gctctttaaa ttcagagagc agtaacatta ctcgaaccga aatttcaaat
    19981 ggagtattaa acaaactatt catcttcagt cacccatttg atctttccgt cattaaccag
    20041 catgtggcaa atgccttttt tagcagaatt ttgcagcaat cccggtataa acgataagat
    20101 tgatccgttt aatgttataa cagacgactg ctgtagaacg gctttgagtc tctcaacgcc
    20161 atcatcgtag tcttcttcat agacttcctc gatgccgtcg tacatatctt cttttaaagc
    20221 ttcaaagtgc tcagttcctt cctcttgtgt aaagttatca cggaccccac gacgtatgct
    20281 ttcagcttta tagtaatttc ttcgctgtct cttgaaatcc tttagccgcg aatttggcag
    20341 gtcgtttgta tgctcgtata cttcgtatcc atcggcatct gcatatgctg aaaaaagagc
    20401 tatgacatac gtcatctcct cagctctaat atcaagggga actggcaaat cgtcaaagaa
    20461 ttttttcact tcatcattat aagagaaatc accattagtg ttgattctta aacttgcgag
    20521 ctctaattcg ctaagcttcg gcttggcctt ttttgttttt acttttgttt gagaagcaat
    20581 tgaggtgatt tcatcaagta aaagatgggc cactatttca gaaacgtctg tatctttggg
    20641 gattaccaca cctttatcaa tcaattgttg cttaaggacg gttggagcat catctataag
    20701 acgttcataa ataaaagtcg tgcatttgtc gatgttgaat tttccagcaa cccgaactgc
    20761 atactcttgt gttaatggtc gagtaccatt gtaaaatctt cgtaggttat catcagaaaa
    20821 accgaaaatg gtggcctcgt catcatttga aacactatcc agtaaagcaa ttaaaaaatc
    20881 ctgtggcttc acgtactcgc ttcccataaa cggataaagc gtttgagtaa tttcacttat
    20941 ttccaaaata caacctcctt tgttacgtcc gccgttgacc gccgtttgcc gccgatgact
    21001 gacgaaaaca agggaggcct ttttctataa ttaaaacata gtagtttaac tatttaattt
    21061 tatcatttat ggttcataaa cactacatta tgtttcaaga aagtgaacga atttatctta
    21121 aaagcgaata gtcctactaa taaaatataa caatgcctga gttgcaatag ggcaaggtta
    21181 caaatactga aaagaactgg ttatccagaa aatttttcag atattgtatg ccttacttta
    21241 ttgcgctctt ttttcgtgat gagggacggt gtacgtattg gcacggtccc tgtttgtttt
    21301 ccttgctcaa aagctctttc aggcaagaaa gagggatcta atgagtagat ttatgaaaac
    21361 acaccaaaac gaccgcagca cttacaagta cgtgtttgag cgtactgatg ccaatggacg
    21421 tatccaaaaa gaagtggttg agattcgccc aggcgaagac ggcgtcactg agcttaacat
    21481 tcaggagctg catcgcgctg acgaccgtgc gatttacaac ttttataaaa acgcacgtcc
    21541 tgaacgcaca gaagaagaaa aagaaaagat caaggcttgg aaagagaagt tccgtgaaga
    21601 ctttaataat gagcacggct attatccaat ggacgaatac gtggatggtg aagccaacga
    21661 ttacttcaaa cgtaactaca atttgtcgct caatgaattt gatgatgaat gcgatgaggg
    21721 ggacaacccc atccaatcat tggtttacga tgcttaccaa gataatgaag aagatcctcg
    21781 tatcgatcgt ttgcgtgaag tcgtgacgca attattaccg taccaccaag aaatctatca
    21841 catgctcgtc actgaaaaca tgtctcagac tgaagtggcg gacaagcttg acaagaaaaa
    21901 acagtcaatc aatcgcacct ttggaatcat caaagacaag atcaaagaat tattttagaa
    21961 aaaagttacg ggggagttta ctttccccgt tttccttcgc ctgtattgtg taagggagta
    22021 gatcctaaac aaataaagat gaaggaggac catcatgaac ctaaaacaca aaatccgagt
    22081 aaacgtgact gatgacacgg gtaacaaaga aactgtccta caaggtggtg tgcgtcattt
    22141 gcctcgctgg tttgcacggt ggctgttcgg taagggcaac atggacaaaa ttctcgtgct
    22201 cacaccaggg gacacaatcg aatcagtcga aatccgtgaa gttaacaaga agggagcgta
    22261 atgacgtgag taaaacaaaa ctatttcttc aggtcgttga ggacgtccaa gcgttagctg
    22321 atagcttaca agagttggct gtggcgatga aaaccaatga acctgaacca caagttgaaa
    22381 caccagaacc gacaatggaa acaaaatcag aagtcaaaca accgacgctt gaagaagtcc
    22441 gtggtttgtt ggcacgtaaa tctcaagaag gtaagtcaac tgaagtgaaa gcactgattg
    22501 agaaatatgg tgccagtcgc ttgagtgatg tgccgacaga aaactacgcg gctttggttg
    22561 cagatgcgga gggattgtag atgccaacac aacacgcagt cctatccgca tcttcaagta
    22621 atcggtggat tcactgtccg ccaagtgttc gcttgtcaga gcactttgaa aacaagtcga
    22681 gcccgtatgc ccaacaaggc actgatgctc ataccttatg tgagtacaaa ctgaataagt
    22741 tcattggtaa tgacgtcaca gacccaacat ctacgcttga attctacgac gaggagatgg
    22801 agcagtgtgc tgaaagttat gcgaccttcg tcatggaaga agtcgctaag gcgaaacaga
    22861 caacagttga ctctattgta atagtagaac agcggctcga cttctcaagg tacgtcccag
    22921 acggctttgg gaccggcgac tgtctcgtca ttgcggatgg aaccctatct gttattgaca
    22981 tgaaatacgg tctaggaatt ttggtagatg cgtatcagaa cccacagatg atgtgttatg
    23041 ccttgggagc gttggagctt ttcgatgtta tttatgacat tcaagaagtc aagatgacca
    23101 ttttccagcc aaggcgtgag aatgtgagta cgtacacact gctgaaagaa gaattacttc
    23161 agtgggcaaa agatgtgttg tcgcctgccg cagatttagc tttcaaaggt gagggtgagt
    23221 accaatgcgg gaaatggtgt ggattctgtc cggcgaagaa tagctgtcgt gcacgagcag
    23281 accataactt gaaactggcg caatatgagt tcaaaccacc agagcttctt agtgatgatg
    23341 aaatcgaaga aatcatcggt aaagtggatg acctggtttc ttggagtaac gatatcaagg
    23401 actgtgcttt gaaacttgca ctcggtggca aacaatggac tcatcacaaa ttagtcgagg
    23461 gtcgctctac tcgtaagtat tctaatgaca atgatgtcgc tgcggcggtc atcaaggctg
    23521 gatacgaccc atatgataaa aagttacttg gtgtcacagc aatgacgaag gcactcggta
    23581 aagagaaatt caatgaaatc ttgggcgaat acatcatcaa accaaaaggc aagctgacgc
    23641 tagtcgatgg ttcagataaa cgacaagcgg tcacagtaaa caacttaaac gaagaattta
    23701 aaccattaac ggaggaacaa taatatgtct aaaacaagca aagtaatcac tggtacaaac
    23761 acacgtctct catacttcca cggttgggaa ccagcatcca tcaacggggg cgcagagaaa
    23821 tattcaacag cagtcttgat tccaaaatca gataagagaa cgattgatac cattgaaaaa
    23881 gcaatcgatg cagcaatcga agagggactt gcgaaattcg gtgggaagaa accaaacaaa
    23941 gcagaaatca agctgccatt acgtgatggg gatatcgaac gcgacgatga agcataccaa
    24001 ggccattact tcattaatgc gaactcaacc actgcaccac aaatcgtgga tcagcacgtt
    24061 caaccaatct tggatcgttc acaagtgtat agtggttgct acgctcgtgt gtcgattaat
    24121 ttctatgcct ttaacgcaaa tggaaatcgc ggggtagcgg cagggcttgg gaatatccag
    24181 tttgtacgtg acggcgaacc actcggctca caagtatctg caagcgatga gtttgaaacc
    24241 ttggcggatg atgatttctt ggcttaatca ttaaaacaaa aacggccatg gcagaagtgc
    24301 tgtcgcctgt ttgattggag gaagaaaatg aaacatttgt cattggattt ggaaacgtat
    24361 agtagtgtcg atttatcgaa atgtggtgtc tacaaatatt cagagtcccc cgattttgaa
    24421 atactgctat ttgcatacag tgtagatggt ggtcccgtcg aagtggttga tctgaagatg
    24481 gacgaaacta tcccggctga aatattaact tcgctatcag atgaaacagt gattaaacac
    24541 gccttcaatg cgagttttga acgtgtgtgt ttgtcacgct ttctggggtt ggatggcttt
    24601 ttgaatccac gagggtggca atgcacaatg gtgtggggtg cgacattagg tttgccgttg
    24661 tcacttgtag gagttggtgc tgttcttggt ttggataaac aaaagctcac agaaggaaaa
    24721 gacctcatcc gatacttttg cgtgccatgc aatccgacca aagttaatgg tggacgcacg
    24781 aggaacttac cacaccatgc gccaactaaa tggtcagcat ttaagacata caatagtcgt
    24841 gatgtggaag ttgaaatagc tatccaagga aaactttcca attttccagt gcttgattct
    24901 gtttggaaag aatactggct ggatcaggaa atcaacgacc gaggaattga gattgataag
    24961 gactttgtgg aagctgccgt tcagttggat gaattatcaa aagctggatt gaatcaacaa
    25021 ttgcaagaat taactggact agaaaatcca aacagcgtgc ttcaaatgcg gggatggtta
    25081 ctagaccacg gacttgaaac cgagtcactc ggtaaaaagg cagtggccga actactcgaa
    25141 caacctattg atgaaaattt aaagcaagtc ctggtcttac gtcagcaact cgccaagtca
    25201 tccgtgaaaa agtatcaagc catgcaaaac tttgcgtgtt ctgataatcg ttctcgtggt
    25261 ttattccaat tctatggagc caaccgtacc ggacgctttg caggacgtgg catccagctt
    25321 caaaacttgc ctcagaacca tatgtcagat ttatctgaag cgcgtagttt agtaaaacaa
    25381 agcaattttg aaatgcttaa cattctctac ggcaatgtcc cgaacgtact ctcagaactc
    25441 ctccggacag cgtttgtacc acgtgaaggg atgaaattta tcgtatctga cttctcagcg
    25501 attgaagcac gtgtgattgc ttggctgtcg ggtgagaagt ggcgtcaaga agtattcaaa
    25561 aatggtggcg atatttactg tgcgtctgcc agtcagatgt tcggtgtgcc agttgaaaag
    25621 catggcgtga atgggcatct acgtcaaaaa gggaaaattg ctgagcttgc tctagggtac
    25681 ggaggatcgg tgggtgcatt gaaagcaatg gatgctcttg aaatgggact aactgaggat
    25741 gaattacagc cgcttgttaa ttcttggcga gactcaaaca ttaacataac ggagctttgg
    25801 tgggctgttg ataaggcagt aaagacttgt gtgaaagaac gacggagcac agcgacgcat
    25861 ggcatcaagt ttgaatacaa gtcgggtttt ctgttcatcc aactcttgtc aggacgtaaa
    25921 ttagtttatg taaaaccaaa gattggtgag aacaaattcg gcggtgaagc tgttacctat
    25981 gaaggtgtcg gtgctaccaa gaaatgggaa cgtcttgaaa gctatggtcc caagtttgtc
    26041 gaaaacatcg tgcaggcaat agctagagac attctctgtt tcgcaatgga gaatcttcgt
    26101 aagtatcgca ttgtcgggca cgtccacgac gaagtgatta tcgaagtgcc aatggaatcg
    26161 aaggttgaag acatctgtca gttgatgagt caaacgccta agtgggctga aggcttgcta
    26221 ctgaacgctg atggatacga gtgtgaattc tatcaaaagg attagtcaat gtggagtcag
    26281 aaatggctcc atattttttt caaatttttt tatcaaaaat aaggttcgaa ttgggttctt
    26341 tcttcgctta tagacgaagg caaaaaaatc aaaaataaat tcgagtcaga gtttactttc
    26401 gaccattttc ttcgcctgta agttagaagg ggcaaaatgc ctctcctgaa aattctatta
    26461 caggaggttc acaagatgaa cgaattgaaa gtttttgaaa atgcggagtt tggctctgta
    26521 cgcacggtga tgattggcga tgtccctcac tttgttggta aagatgtagc atcaattctt
    26581 ggctacacaa atccacaaaa agcattacga gatcatgttg atgaggaaga taagacggtg
    26641 aacgaatcgt tcagcgttaa tgggacaatg gctatcctta tcaacgaaag tggattgtac
    26701 tcactcatca tttctagcaa gatgccgaac gcgaagaaat tcaagcgttg ggtgacaaat
    26761 gaggtcttgc cgtctatccg ccaacacggt gtgtacgcga cagaagattt catcacaaag
    26821 tcaatcgaag atccagcatg ggccatttca gtgttgaagc agctacaaga gaaaaaagaa
    26881 atggtggcga tgcaacaaca aatgattttg gagatgaaac caaaagttag ctactacgat
    26941 ttgattcttc aaaacaattc tgtgatgtcg atttctaaaa tctcgaagga ctacggtatg
    27001 tcaccacagg caatgaacaa gttgcttcat gaactgggaa ttcagtacaa gcaaggcaag
    27061 atgtggttgc tttatcagaa gtatgccaat caaggctaca cacaatcaaa aactcatgcg
    27121 atagatgcag agcgcagcgt gatgcatacc tactggacgc agaaaggtcg tttgttcttg
    27181 tacgacttgc tgaaatcgaa gaagaacatc ttaccagtga ttgagcgagg tgataataat
    27241 gcctagacgt aaagattaca gaacatcagt ctatgtctgc tcgccgttct caggaaatgt
    27301 tgtcgtcaac cataaaaaca ctaagcgtta ctgccgtatt gctcttgacc gtggctacat
    27361 gccagttgcg gcacatctga tgtatccaca attcatggac gacgcaacgg agcgtgatgt
    27421 ggcaatgcac atgtcacttg ttctcatggg caaatgtgag gaagtctggg tggtgggtga
    27481 caccatctcc caaggcatgg cagttgaaat tgaacaagcg gagtattgga agaaacatat
    27541 caggtacttc gatgatgaga tgaaggaggt ctcaaatgct taagatgacg ttattcacat
    27601 ccaatcaggt gggtaattcc aagaatggat tgttcccaac caagcaagtg gtgacagata
    27661 aagaaacact tgttgccacg gtcaagctgg atcatgtgat ggctgagtac aaaggcaact
    27721 atcgcaaagg tgacaacttc atccagtctg atgtgattcc acttgattgc gacaatgacc
    27781 actcagacaa tccagatgat tgggtagccc ctttcgaagt ggcgatgcag tttcctgatg
    27841 tgacgtttgc ggtcagctat tcacgtaaca acatgaaaga caaaggtggc aaatcagcac
    27901 gtccacgttt tcacgtatat ttcccgattc ctgccacaac tgataaagat gaatatgtgg
    27961 aactcaagaa gcagattcaa agtgagttcc catactttga caacaatgcg atggatgcgg
    28021 cacgtttgct tttcggaaca ccaaactctg aagttgaaat ctatgaaggt agtaagttga
    28081 tcactgattt tctcgaagat gatctgtttg ctgattggga taatgcgcag tcagagatta
    28141 gcgaaggcag ccgtaattca agcatgagtc acatcgcagg aaagctcatc aaacgctacg
    28201 gtgcaacgga agatacgtat cacaagtatc tcgaacaatc ccaaaaatgt aatccgccac
    28261 tgccggagga ggagcttaaa gctatctgga atagcgcggt caactttggg aagaaagtga
    28321 aacaacaaga aggctacatt cctccagagc agtacaactc tgatttatca cttgagccaa
    28381 ccgactactc ggacttaggt caagcaacgg tcttagctcg cgagtatgaa tgtaagcttc
    28441 attactcacc gtcaactggt taccttgtct acaacggcag ctattgggaa gagtcgaaac
    28501 caaaagcgca aggtgtgatc cacgcattga cagaacgtca actggaagaa tctgaaaccg
    28561 aaattgagaa gcgtaccaag gaaatggtta gtaatggcgc gttcgatgta cttgctagtg
    28621 ttggtccgaa aaaggcagtc accatgttca acacagcgca acgtcatagt tttgatttgt
    28681 accaacacgc acaagagtat aagaagtttg ctgtcaaacg ccgcgacagc aagtatctct
    28741 cgtcagcatt aaccgaggca aagcccatgc ttgaaatcga gcaacggtta ctagatgtga
    28801 acgagttctt gctgaacaca ccaacagcaa cctttgattt gcgaacaggc aaatcacaag
    28861 accacaactc agaagattac atcacgaagc aaacggaatg tgccccgagt gacgccaatc
    28921 agcagatttg gttggacgct ctgaacacca ttttcgtagg tgaccaagag ttgattgatt
    28981 atgtccagat gattgtagga cttgcagcta ttggtaaagt ttacgttgaa gcgcttatca
    29041 tctcgtatgg tgaagggcgt aacgggaagt ccacgttctg gaatgtcatc agccgtgtgc
    29101 tcggtaatta ctctggcagc atctcagctg atattcttac aagtcaaatc cgacgcaatg
    29161 tgaaacctga acttgcggaa gcgaagggga aacgcctact catcgcagcc gagcttgaag
    29221 aggggatgcg actcaacaca tctaacatta agcagctctg ctcgacggat gaaattgcgg
    29281 ctgagaagaa atacaaagac ccattcaagt atgtcccaac gcatactttg gtgctctata
    29341 ccaaccacct cccaaaagtt ggagcgattg ataaaggaac ctggcgtcgt ttaattgtga
    29401 ttccattcct tgctacgatt gacggcaata aggacatcaa gaattatgcg gactaccttt
    29461 ttgaaaatgc cggcggagcc attttgcaat ggatccttga cggtgcgaaa aaagtcatcg
    29521 ccgcagactt tcatttaacg gttccacaag ttgtcgctaa tgcaatttct gaatataaga
    29581 cggcgaatga ttggcttggc catttcttag acgagtgctg cgagactgct gttgactttg
    29641 aagaaaaatc tggtgagttg tatgcagagt atcgtgcgtt ttgtacgcga actggtgagt
    29701 acattcgtag ttcagccgat ttttacacgg ctctggaggc gggcggattt aagcgccggc
    29761 gaactaataa aggaaacaag attgttggtt tgcaactcaa atccgagttc ttggaatagt
    29821 tgattttaaa ggaaagtgta acctgtgtag gtcttatata gaacttttct taagggataa
    29881 aaaattacca cctatataaa gttatgtatt gcgtattcac gggttacacc atacttgcag
    29941 attggagaat atctaaatga aatttaaaca gtggttacaa attggaatcg gtaaacgtga
    30001 tttacgattt gatgagtttt acgagtttgc catgtcggat gtcacgtatc cttggaagaa
    30061 atcattcgat aagcaagtcg cacatctgat gattcactgc gcagaaaagg aatacttgga
    30121 aatcttgcgt gatgcgtatt tcgagtattt gacggtgtgg ctatgagaga aaagcaggtt
    30181 gagcaagcac tagtgaaagc cgtcaaaagg gtaggtggta tttgcccgaa gtttacatca
    30241 ccggggcttg ctggtgtgcc tgaccgattg gtacttatgc caaatgggaa acttggattt
    30301 gtcgaagtga aggcgccagg gaagaagcca cggtctttac agctttttcg tatgaaacaa
    30361 ctgacagatt tagggtttca gtgtttcgtg cttgatgaga ttgatcagat acctgagtta
    30421 ctagaaagaa taggaggtga tgtctgatga agttcgtacc acataactac cagaaatatg
    30481 cgattgattt tatcaaagat cataaaactg ccgcccttct attggacatg ggcttgggta
    30541 aaacggtgac aacgctgaca gctattaaag acttgatgca tgatgacttt acaatacaac
    30601 gtgtcttaat tattgcaccg ttgcgcgtga cgcaatcaac atggccaact gaaattcaaa
    30661 agtgggatca tttgaaagat ttatcgtatt ccgtggttct tggaacacca agtcagcgaa
    30721 tggcagcatt acaaaataat gccaacatct atctgatcaa tcgtgagaac ttggactggc
    30781 tgattaacaa atcgggtttt acgtttgatt ttgatatggt ggtgattgac gaactgtcgt
    30841 ccttcaaaaa cttaaaagct aaacggttca ctagctttat gaaagtcaga cacaaggttg
    30901 accgaattgt tggattgacc ggaacacctt caagtaatgg tttgatggat ttgtttgcgg
    30961 agttcaaggt tctggatatg ggcgagagat tgggctatta tatttcacgt tatcgagata
    31021 aatactttct acctgacaag caaaatggca tgcagattta ttcttggaaa ccaagggaag
    31081 atgcagaaca agaaatctac gacaagattt cagatatcac gatttcaatg aagtcagtcg
    31141 atttcttgga tatgccagag cttgtgatga atgaagtgcc agttgatatg ggactcaaag
    31201 aaaagcaaaa gtacgatgaa attaaggcag acttggtgct acaattgaaa gaagccacca
    31261 ttgatgcagc caatacagcg gtgctttcga ataaactgct acaaatggcg aatggagcaa
    31321 tctatgatga gtttaatgtg agtcatcaca tccatgacca gaagttagat gctttggaag
    31381 atttgattga aggtacaaat ggcaaaccga ttctgattgc ctattggttc cagcatgatc
    31441 tggagcgtat caaggaacga ttcaacgttc gacagatcaa aacgtctcag gacattattg
    31501 attggaacga aggtagcatc cccatcgctg tcattcatcc ggctagtgca ggacatggat
    31561 tgaatttaca agctggtggt tcaacacttg tatggtttgg tcttacgtgg agtttggaac
    31621 tttaccaaca aaccaatgca aggctgtggc gacaagggca aagtgatact gtggtcattc
    31681 atcacattat tgcgaaagat acgattgatg aagatgtgat gttggctttg aaacgaaaag
    31741 ataaaacaca atcatgtttg attgatgcag tgaaagctag attggaggga agcaagtgac
    31801 accaaaagaa tacttgaaac aagcgtaccg tttgaataaa cgaattgacg accggattaa
    31861 agagaaggcg gagctctatc aactggcggt gacagtttct tccacaacat tcgaagaaaa
    31921 gatttcaagt ggcacacgga atactgaggc accatttgtt cgtaaccttg agaagtgtta
    31981 ccaattggaa gctgaaattg atgcagagat tgataagttt gtagacctca aacgacaaat
    32041 gcgtggcgtg attgatgaac tcacaaacgt ggacgaaaag attgtgttaa tcaatcgata
    32101 cctgttggaa gagagctggg acaagattgc tgaagatctt cacgctgacg tgcggacagt
    32161 ctttcgttgg catagcaaag ctcttgagaa cttgaagcta ccagatgaac ccatagtcat
    32221 ttgaaatgtg tcagtgaatg tcagtagcct ttagtgatat cattaagatg tcaaaaaaga
    32281 caaacgatac tcaagcaata ccagcaatag aagtatcgaa caaatactcg aagcctttgt
    32341 gggaaaaatc cttcaagggc ttttcttttg cccgaaagga tgtgacccca cttgccaatg
    32401 aaaccagcta acccctgtaa gttcccaggt tgtccgaacc gtacgcatga tacttattgt
    32461 gatgaacata aaacacttgc gaggaaacgt tacgagaaat acgagcgtga tccggcaatc
    32521 aacaaacgtt acggcagagc gtggaagaaa atacgggcaa ggtacgtggc agcacatccc
    32581 ctatgtgaga tgtgtctgaa ggaaggacgc tttacttcgg ttgagattgt gcatcacatc
    32641 aaggaattaa gtgatggtgg tacgcatgat gaaaataatt tgatgagtgt ctgcaaatca
    32701 tgtcactcga aaattcacat gactcgacga aacacaaaag ataaaacaat catgtgaaaa
    32761 ataacaaggg ggggaggggc ggtaattatc cctaaaccct tctaaactgt acagcgcgct
    32821 aggcccttac gcgaaaaaag ttcagttcaa acgggggata gccccaaaca agaaaaagag
    32881 gtgataaatt ttggcaaaag atggaacaca acgcggtggc agacgacctc gttcagggga
    32941 acgctcacag ccactcgttg ataaagttgc tgctggaaag aaagcatcgc ggatggaatt
    33001 tgacttaccg gatgttgaat tattaattgg tgaagatatt ggtgagggtg tagagcttga
    33061 gggcaatgat tatcctgaac caagtgctta cctttctgca aaacagaagg atggcactgt
    33121 gctaggcgct gatgaaattt ataactcaac gttccaatgg ttgaaagacc gcggatgcga
    33181 aacgttgata gccccacgaa tggttgagtc ctacgctcag aactttgcgc gttttgttca
    33241 atgtgaaaat gctctgagtc aatatggttt gctcggaaaa cacccgacta ccaaggcagt
    33301 catggcctcc ccatttgtgg cgatgtcgca gaacttccaa aagcaagcaa atgtgacgtg
    33361 gtatgagatt caacagattg taaaagagaa tgcatcagtg gaatacacag gagatccgaa
    33421 tacggatgcg atggaacgat tgttacgttc taagaatggt tgaaaggaac agaatttatg
    33481 atagagaagg ttaatccgca acatccagat aaagttgcag atcgtattgc cggagccatt
    33541 gtggacttgg catacgcaga agaaaataat ccaaagatag cagttgaggt tttaattggt
    33601 catggcatct gtcatgtgat tattgaaact tcaactgctt taaatttgaa tgaaatcagc
    33661 caagcgatta atcgtatcgc aggcaaaatt aaaaaagatg tggtgattgt gccccaagat
    33721 aaagagttgg ctgacaatca agcgactgaa attcgttgtg gcgataatgg aatctttcgt
    33781 gggatgccat tgactgatga acagttacag ctctcaaaaa ttgcgcgtga cttatatcag
    33841 gaccatccat ttgatggaaa gtacattctt gatggtgaac gcttgataat ttgtcagagc
    33901 cacgcaaaga caaatgagtt aaaacaaaaa tatccaaacg ctacagtcaa ccctctaggt
    33961 gattggactg gcggcccgga tgtggataca ggtgcgacta atcgcaagct tggttccgat
    34021 atggcagacg gagtaacagg tggcggtatc catgggaaag acttatcaaa agctgatgtg
    34081 tcagtgaaca tctacgcatt cttgaaagct caagaaactg gtatgcccgt tgagttaagc
    34141 tgtgcgattg gtgacactga gattgatggc gtgccttatt ctgaaatcgt ggaagttgcg
    34201 cgtgattatg tgaatgagct tggcgggttt gagaaactag cagaatgggg gttgttctaa
    34261 tgaacacaac agaacgtttt gagaaagttg atattgataa gctggtccct tatgccagaa
    34321 atgcccgtac ccacagtaag gatcaagtga aacagattca agcttctatg cgtgagtttg
    34381 ggttcataaa tccggtgatt gttgataagg attataactt gattgcaggt cacggacgta
    34441 ttcttgcggc taaagaagaa ggtatcaagc aggtaccgtg cgtgtttgtg gaacacctaa
    34501 ccgaggcaca aaagcgtgcg tacatacttg ctgataaccg actagcaatg aacgccggtt
    34561 gggatgatga aatgttggcg gttgagttat cagacctgca aggtgaagag tttgatttgg
    34621 atttgcttgg ttttgaaact gatgaacttg ataaattact caacggtaat gatgatgccc
    34681 aagatgatga ctttgatgta gatgaggaac ttgaaaagcc agccttcagt aaaacagggg
    34741 acttgtggtt gcttggtcgt caccgtttag tttgtggtga ctcaacaaaa cctgaaactt
    34801 atgagaaact catggacggc aaactagcaa atcttgtggt gacagatcca ccttatgggg
    34861 tctcatatca aggtaaagcg ggaacgatta aaaatgataa tctgaaggac gaggcatttt
    34921 acaacttctt gtatgatgcc ttttcttgta tggaaagtgt gatggccaac gacgccagta
    34981 tttatgtttt ccatgcggat acggaaggct tgaacttcag aaaagcattc gtggatgcag
    35041 ggttctattt atcaggtacg tgcatctgga agaaacagag tctagtgctt ggtcgttcgc
    35101 cttaccaatg gcagcatgag ccggtgctct atggctggaa gaagaaaggc aaacatcaat
    35161 ggtacaccgg acgtaaggaa tcgaccatct gggaatttga taaaccaaag aagaatggtg
    35221 accacccaac gatgaaacca attccgctgt tggcatatcc gattaccaat tcgtcactca
    35281 gcaactgcat cgtgttggat agttttggtg gcagcggatc aactttgatt gcttgcgaac
    35341 agaccgaccg gatttgccat acgatcgaac tggatgaaaa gttctgtgat gtgattgtta
    35401 agcgttatat cgagcaggct ggaagcgatg cagacgtttc agtgattcga gatggaaaga
    35461 ctatcgcatt taatgaccta gaaaatcctg aataaataac gtatcacttg ctataaaatc
    35521 cctttagagt gatatataga ctaccaaaag aatacggaag acaaagttat gtttccaact
    35581 catgaacaaa tcgagggtat gaaaaaacgt taccctgaag gtagtcgtgt agaactcatc
    35641 aagatgaacg acccacatgc cccagcgaag ggcacacaag gcacagtaac cggtgttgac
    35701 gacattggtt caatccttat ccaatgggac aacggttcaa cactgaacgc ggtatacggc
    35761 gaagatgtgg tgcacatcat caaaccagac ttcgaattgg tctttcaaaa cggtagaaga
    35821 gaaggatttg aaaccttcaa tgatgcttgg caatacgtga cggacatgat attgaaccac
    35881 gatttggtct gggttgacct tgagtcaaaa acagaagata ccatctacat cagaaagggg
    35941 ctttaagatg aaagtgagta aacgtaagat ttacaacatc gcaaagaatt acatcgttca
    36001 gttgcctgaa cgtggcgacc tgaaggccca caacagcgac cgtgaagact tcttggacat
    36061 tgcggtttgg tgtttggaag atgccttaat tgcggcctat gaggcaggca gaaaggacgg
    36121 tcaaaatgaa cgcaaagatt aagcaacaaa tcattgagat tgcagaaagt ggtctgacaa
    36181 atatgtttga tgcgattgca gtgcagcgaa ttgcacatga ccggcacttt tacgagttgg
    36241 tgattttcat tgaagaacac aaagatgagt acagccgctt tatcttggag ggtgataaat
    36301 gatggattat caaatttaca tgccagataa tgaagatggc attgaagaag gatactacga
    36361 ttggcaagaa ctggtgcaat tgctgcggga taacaagaac aaccctgaag ccattcagtt
    36421 cattgccgat atgatggaat aaagagatta aggcagctga caacagctgc tttttgcgta
    36481 gaagggagtg agttccaatc gataattaca aaccaactga atttatggac aaagactccc
    36541 attatgataa gtcacgtgca gattatgccg tggcctttat tgagttgttg aaacacacga
    36601 aaggtcagtg gttcgggaaa ccgttcaaac tgatgccttg gcaagaaaag attatccgtg
    36661 acttattcgg aacgattaag gcaaatggct acagacaatt caatacggct tatgttgaaa
    36721 tacctaagaa gaatggtaag agtgagttag cggctgccgt ggctttgctt ttgacttgcg
    36781 gtgatgggga agaacgcgct gaggtatatg gctgtgctgc tgatcgagcg caggcttcaa
    36841 tcgtatttga cgtagctgtt gatatgattc gccagaaccc agcgttaagc aaacgagtga
    36901 aaatcctgac ttcacaaaaa cgaattattt atcagccaac gaatagtttc tatcaagtat
    36961 tatcagctga agcatactct aaacacggat taaacagttc tgcggttgta tttgatgagc
    37021 ttcacgcaca acctaacaga aagctgtttg atgtcatgac gaaaggggca ggggatgcta
    37081 gaacgcaacc gctattcttt ttgattacaa cggcagggaa tgataccaat tcaatttgtt
    37141 atgagcagca tcaaaaagct ctcgacatta ttgaaggtcg aaagcatgat aaaacattct
    37201 atccgttaat ttacggtatc aagcaagatg atgattggac tgatccagaa gtttggaaga
    37261 aagccaatcc gtcacttggg ataacgattg cagaagaaaa ggtggtggct gcttgtgatt
    37321 cggctaaaca aaatccagct gaagaaaact cattcagaca gcttcgcctg aaccaatggg
    37381 tgaaacaagc agtgcgttgg atgccaatgg ataaatggga cgcttgttca ttcaatgcag
    37441 atgaagaaag tttgagaggt cgagtttgtt acggaggtct cgacctttcc agcacgaccg
    37501 accttacatc cttcgttttg gtgtttccac ctgaagatga ggatgacaag tttgtgatac
    37561 tgccatatta ctgggtgcca gaagatacct tggacttacg agttaaacgc gaccatgtga
    37621 attatgattt gtgggccaag caggggtaca tacaaaccac tgagggaaac gtagtccact
    37681 atggtttcat tgaaaaattt attgaggaac tgggtgagaa atacaatatc cgggagattg
    37741 cctttgaccg ttggggagca gttcaaatgg ttcaaaactt agaaggtatg ggatttactg
    37801 tagttccttt cggtcaaggt ttcaaggata tgagcccggc tactaaagag ttgatgaagt
    37861 tgactcttga acagaaatta gcccatggcg gtcatccggt tcttcgttgg aatatggaca
    37921 acattttcgt ccgaactgac ccggcaggta atatcaaagc tgataaagaa aaatccactg
    37981 agaagattga tggtgccatc gcgacaatta tggctctgga tcgtgcaatt cgttgtggaa
    38041 atgataatgg tgccagtgtt tatgatgata ggggattgtt gtttctgtga aatatgagct
    38101 tttattacaa tccattgata taattgtctt tggaggtgta tgtgtatgaa tgaatatttg
    38161 attcaagtag caccagataa aatatcttca gacccggaca acacattaat ttctagactg
    38221 ttttatagaa aatggtgggg aaaaattcta actgaagatt tgacaaagta tgctgaagga
    38281 attgggaatt ataggggaga aacgattatt tctttcaaca caattgctgg tgcaacgata
    38341 cggttattga aatgctataa tcaaaagaac ttttcaatgc cagatagtca ttatgggcgt
    38401 ttgaaagtca ttttatattc cgatgatatc accgaagaga tgaaagtgaa atttgtaaaa
    38461 ttctatgatt taagtcatac tcttgcaaat tttatgccac tatatgtatc aaaaccttct
    38521 ctcaatggtg ttaaaggtaa agggccgtat catgactttc ctgatttatt ttattctgat
    38581 gtaagagata aacgatatgg ttcatcaact aacgctgata aatttatcgt taataataaa
    38641 gactatttct ctaaattcaa agattggaaa agttttatag aactaaatta tttacaagat
    38701 ttcttcgatg atgaagatta tatgacgttt aaaaaacttg caccaagtag ttcattcttt
    38761 ccttatcaaa gaaaatcctt aacacctgac gaaaaatcga aatgtttaga ggaagtaaat
    38821 acctttttaa ataatgcaat tgagataatt ttgtcaagag gaagtcggtt ttatatctaa
    38881 tgtgaacaca gcatctacaa atcgtaggtg cttttttgat actcaaattt tgaaaggagc
    38941 aatcaatggg cataataaaa tcactattca aatcacgcga taaaccaaca aatcaaacca
    39001 tcatgaatcc ataccgattt atgtttggcg gctcatcagc tggtaaaagc gtgaccgaac
    39061 aaacagctat gcagatgacg gctgtgtact catgtgtgcg cgtgctatca gaagccgtgg
    39121 caggattgcc attacacttt taccaatacc gagatgctgg cggcaaagat aaagcaatga
    39181 aacattcgtt gtatttcttg ctgcatgatg aaccaaattc ggaaatgaca tcatttgtat
    39241 ttcgtgaaac cttaatgact cacctgctac tttggggtaa cgcctatgcg caaattatcc
    39301 gaaatggcaa aggtgaagtt gtagctttgt atcctttaat gccagataag atgacggtta
    39361 atcgcgatga caacaaggaa atctattatt tatatcaagc tgatgtcggg caagtcagac
    39421 tttcaaaagc tgacgtgctt cacattcctg gccttggctt tgatggcttg gttggttatt
    39481 caccaattgc catggctaag aatgctatcg ggcttgcgat tgcaacagag gaatatggtt
    39541 ctaaattttt tgccaatggt gccaatcctg gtggtgtcct agaacatcct ggaactttga
    39601 aagacccagg aaagcttcgt gaaagttgga atgcgacatt tggtggcagt tcaaatgcgg
    39661 ccaaggttgc agtacttgaa gaaggcatga agtatacgcc tatttccatg tcaccagaac
    39721 aagcccagtt ccttgaaaca cgtaagtttc aactggaaga aatcgcacgg atattccgag
    39781 tgccccttca catggttggc gacttagacc acgcgacctt ctcaaacgtt gaacatttga
    39841 gtttggattt tgtgaagttc tcattatcac catggctcaa tcgttgggaa caagcactta
    39901 ataaatcact gttaagtcgc gatgaaaaag aaaagtattt cataaagttt aatgtggatg
    39961 gcttattgcg tggtgactat gccacacgtc aacaaggcta tgcaaccggt attcaaaatg
    40021 ggttcctatc tcctgatgat gtgcgtgaac ttgaggactt gaacccaatc cctggtggtg
    40081 ctggtgcgaa atattacttc aatggcaata tgctgccaat tgaacaagcc ggggcagctt
    40141 atggcgtaaa caaagaagaa agcgaggaac aagatgaaga agttttggaa gtggaagaat
    40201 caaacggttc tgaatcaaga caaccaagag gaaatcctag agagaaccct ctacctcaac
    40261 ggaccaatcg ctgaggagag ctggtttgat gatgaagtca caccgcagat tttcaaagat
    40321 gagttaaatt caggtggagg taacgttaca gtctggatta attcgggtgg tggcgattgt
    40381 gtcgctgccg caagtattta caacatgctt gctgactaca aaggtaaagt cacagtcaag
    40441 attgacggca tcgccgcttc ggccgcaagt gttattgcaa tggcaggtga caaggtactc
    40501 atgagtcctg tggcgatggt gatgattcac aatccgtcag cggttgcgtt tggcgaaaaa
    40561 gttgatatgg agaaagccat tgccatgctt gatgaagtga aagcaagtat tatcaacgcc
    40621 tatgaacgta aaacgaattt atcgcgtgtg caactggcac atatgatgga tgcggagacg
    40681 tggatggacg caaataaagc tgttcaccta ggatttgccg atgaaatcat cggtgagatt
    40741 gacccggata ttccggttaa taattcgatg tactcggaag tcaaagtaac taattcactg
    40801 acttcagcga ttgctaagaa gtgtcgtatt gaaccacctg aaccaaagga acgagaagaa
    40861 attaataaca ctatcccagc cgattcgtta atgagtcggc ttaatttaat gaaaaattgg
    40921 aggaactaac ccatgaacaa aattcaagaa ttgatggaaa aacgtgcaca agcttggaac
    40981 ggagcaaagg cttttgtaga agcaaagcaa gataaagatg gcttgatgtc tgaagaagat
    41041 gccaagactt atgcggaaat ggaaacaaaa gtgaagaact ttacgatgga aattgaacgt
    41101 atgcagtcaa tggaagcgat ggatcgtgaa cttgaaaagc caatgagcca accattaact
    41161 tcgcaaccta tgactactga aaataaaatg gaacctaaga aaacaggacg tgcttcagct
    41221 gaatataaag aaggtgtact tgaagccttg cgctcaaact tccgacatgt ttcaaatgtt
    41281 cttcaagaag gtgtggatac ggcaggcggt tatttggtgc cagaagaata tgactcacgt
    41341 ttgattgacg gcatgacttc tgaaaacatc atgcgtacac ttgcgacaac aattacgaca
    41401 agtggtgaac acaagattaa tattgcaggt tctaaaccag ccgcttcatg gattgaagaa
    41461 ggtggcgagt tgagctttgg ggatgcaacg ttcgatcaaa tcttgcttga tgctcacaaa
    41521 cttcatgtgg caatcaaggt gactgatgaa ttgctttatg acaacgcctt taaccttgaa
    41581 aactatatca tcgaccaatt caccaaagcc ttagcaaacg cggaagaaga tgcgttcttg
    41641 aatggtgacg gtgttggtaa accactcggt atttttgcag caacaggtgg cggacaagtt
    41701 gccattacaa ctaatactca aagtgcagtt acggcagatg atgtggtgaa ccttgtgtat
    41761 gctttgaaac gtccatatcg taaaaacgcg aagtttatca tgaacgacca aaccatcagc
    41821 ttgcttcgta agttgaaaga caacaatggt gcctacatgt ggcagccagc tttggtagca
    41881 ggtgaacctg accgtctgct cggttatcca gtttatactt cggagtttgt tccaaccgtt
    41941 aaggcaggtg cgccagtcat tgcttttggt gatttcagct actataacat tggcgaccgt
    42001 ggcgtgcgtt cattcgacca gttgcgtgaa ctctttgcag gtaatggcat ggttggtttc
    42061 ttagctaaag aacgtgtgga cggtaaactt gtgttacctg aagcagtgca aatcttgaag
    42121 atgaaagcat ctgcctcatc ttaaggagta gcctatgagt gacttactaa ataaggtcaa
    42181 ggcaaacctc atacttgagc ataacgaaga tgatgagctg ttggaacgat tgattctgac
    42241 cgctctttct tatgctgaga gctatcaaca tattgagtct ggctactatt cagaacatga
    42301 aatgccgcca accactgaac aggcggttat tgtgttagcc agtaactttt acgaaagccg
    42361 tgatggttca actggtggct tctttgcgga taacgtcaat gccagtaagc aagtctggga
    42421 aacggtcaat ctgttactca ggctcgatag gcggtggcaa gtatgagttt tggaaagatg
    42481 aatcaacgaa tacaaatcgt taaagtgacg aaccaaaagg atagcgctgg attttcagtg
    42541 aaacaagatg aagttcttgc agctgtccat gcatacaaag aggaaaagaa tgcgactgag
    42601 aaatgggtaa acatggcgac ttttagttca gcgacctgcc tgtttcggat tcgttatatt
    42661 cctaatctag aggttacgac agagatggtt gtcatttcag atcagaaaag gtatcagatt
    42721 gtgtcggtgg agaatgtccg aaatcgcaat atgtaccttg aaatgctgac tactgaaatg
    42781 gaggtgagtg cggatggcta aagcattgat gaagatgcca gaagatttct tgttaaaggt
    42841 ttcaaaacta gaaaacaaga cagatgaaat catccccaag gttttagaaa gtggtgctga
    42901 agtggtggaa ggcaaagtcc gaagtaactt aactgccgtt gttggctcaa atacaaaagt
    42961 accaagtcgt tcaactggtg aacttgaatc agcccttggt atcacacaag ctcgtcaaga
    43021 taaaagcggc aactggaaca tcaaggttgg ttttgatgag cctcgaatcg atggcagttc
    43081 aaatgctaag attgcgaaca ttctcgaata tggaaaacac ggtcagccag caaagccatt
    43141 cttgaaacct gcaaaatcac aaagtcgcaa gtcatgcatt gaaacaatga aatcaaaaat
    43201 ggaaagtgag gtaaacggca tttgaacatt ttagaggaat tgaacagtct ttttgccaca
    43261 cttcatattc ctgttgaaac gggcgagttt agtgataaag cgccagacaa gtattgtgtg
    43321 ctgacaccac tcgctgacag gttcgatgtt tacggggata actttccaac cgttgatgta
    43381 caggaagttc gcatttcctt gtttgtgaaa ggcaactatc tgaacacgca aaaacaactg
    43441 gttcagaact tgttgaaaca cgagtttacg attactgaac gattatttgt aggatttgaa
    43501 aaagatacaa attactatca tgtggcgatt gatgtcgcca agcattatga aatggaggtc
    43561 tgaaaatggc aacgattgga ttggataaat tatattatgc accgattaca gagtcggcaa
    43621 ctactggcga agaaacatac ggcacacccg ctatgcttgc gaaagcaatc tcagctgagc
    43681 tttcggtgga acttgctgaa gcaactttat acgccgatga tggtgcatca gaagtagtga
    43741 aagaatttaa aaatggaaag ttaacgctcg gtgttgatga tattggacgt gctgtggcgg
    43801 cagtgcttgt tggtgcaacc gtggatcaaa acggtgtctt aatttcgaca accgaagatg
    43861 gtggcacacc agttgcgatt ggtttccgtg ctcgtaaatc gaatggcaaa tacaaatatt
    43921 tgtggttgta ccgagtgaag tttgccgtgc ccagcacaag cctagcaaca aaaggtgacg
    43981 acattacatt ctcaacacca acaattgagg gtacagtcat gcgtcgtaac aagttagatg
    44041 ctagtggtaa gcatccatgg aaagcagaag ttgatgaaaa cgatacggga gttgaacaaa
    44101 caactatcaa tggatggtac acgagtgttt atgaaccaga tttcacagca gactaggagg
    44161 caacaatgag tgaagaacgc agtgccaaga ttagccttgg cggagatgaa tatgaactga
    44221 ttttgacaac aaaagccacg aaagaaatcg ccagtcacta cggtgggctt gataaccttg
    44281 gtgagaaatt gttgaaatcc gagaactttg aattggcgct tgatgaaatc atctggttga
    44341 ttacgctgtt ggcgaatcag tccatcaaga tttacaactt gaagaacaca gaaaagaaag
    44401 atgaactcac gactgaatat gtggagttga tgacgagtcc gttgcagttg gctgaatata
    44461 aatcagcaat cactgaagca atgttcaagg gcacagctcg caatatcgaa agtgaaacag
    44521 attcaaaaaa caaagcaggc gagtaagtga tgaagaaaca tttactcgcc tgtattacta
    44581 tgggacagtt caaatgggta tgacgtcaga tgatttctgg tactgctcgc ttggactgtt
    44641 tttggattta tgggagtgtc ataagcaatt tactgggatt agcaagccga aagttgagtt
    44701 attcattgat gatgtgatac cgagtggaat ttaagggaag gtggtgattt atttggcaga
    44761 taattttggt ttaaagattg gcgttgaagg tgaaaaggag tttaagaact cgttgcgtga
    44821 aatcaaccaa tcattcaagg tgctaggttc agaaatgaaa ctggtatcat ccgaatttga
    44881 taaaaacgat aaaagcatca gctcactgtc tgctcgcaac caagtcttaa acaaatcgat
    44941 tgatgcccag aaagaaaaaa tcaccaccct tgaaaatgct ttgaagaacg ctagtgacag
    45001 ttttggtgaa aacgataagc gtactcagaa ttggacagtg caattaaaca atgcaaaagc
    45061 ccaactcaat aatatggagc gtgaagttgc taacaacgag aaggcaattg acgccatggg
    45121 tactgaagaa gttgatgctg ctaagaaaac tgatgagctt ggcaacgaac tcaaggatac
    45181 aggtgacgag gctgaaaagt ctggaagtaa atttgaaaag ttcggtggcg ttctaaaagg
    45241 tatcggggta gcaatgggta cagtggcact tgctgctggc gctgctgccg tggagctagg
    45301 aaaacaagtg gtatctgctt acgcggacta tgaacaatta gtcggtgggg tggatactct
    45361 tttcaaggat gcgtcatcca ccgttcaaaa gaatgccgac aatgctttca aaactgccgg
    45421 aatgtcagcc aatgaataca tggaaactgt cacaggtttc tcagctgcct tgatttcatc
    45481 tctcggtggt gataccaaga aagccgcagg agttgcggat caagctgtta ctgatatgag
    45541 cgataatgca aacaagatgg ggacggatat tgagtccatc cagaacgcct atcaaggttt
    45601 cgccaagcaa aattacggga tgctggacaa cttgaaatta ggttttgggg gcacgaagtc
    45661 cgaaatggaa cgtttgctcc aagaggctga gaagatttct ggtgtcaaat atgatatctc
    45721 gaacttcgct gatgtgactg aagcgattca cgttattcaa accgaaatgg gcatcactgg
    45781 tacaaccgcc aaggaagcga gtgagacgat aagtggttcg attgacggta tgaagtctgc
    45841 aatctcaaac ttgatggctg gacttggtaa cagcagcgct gacattggta tgttgattgg
    45901 caatgtggtg gaacaatttc aaaacgtcat caagaatatt ctaccggtca ttcaaaatat
    45961 cgtgtcggct ttaccggcag tggtggatgc cattattcaa gcattggggc agttattgcc
    46021 aacactctta gaagcagtaa cctcattatt ctcacaagtg cttgaaacga tacttggctt
    46081 acttcccaca cttattccag tggtggttca agccgtttta acgattgttc aaacacttat
    46141 tgagaactta cctttactga ttgacgctgc ctttcaactt gtgacaacac ttatccaagg
    46201 actaggcgaa gcgctgccag aacttattcc aattacagta caggcaatca taacgattgt
    46261 gaacggcttg attgaaaact tgccattact gttggatgct gctttacaac ttatcatggg
    46321 acttgcccaa ggacttatca cagcacttcc aatactgatt gaaagtctgc cacagattat
    46381 tacaggaatc gtgaacttta ttatcagtgc tattccacaa atcattcaga ctggtattca
    46441 acttttgaca tcacttgtga cagctttgcc ggatatcatc aatgcgattg ttgcagctat
    46501 tccagtgatt attcaaaata tcatcaatgc cgtgattggg gcaatccctc agttgattga
    46561 cgcaggtatt cagttacttg tggcattgat tggtgcgtta ccgcaaatta tcacaacgat
    46621 tgcgaatgcc atgccgcaaa ttatcaacgg aataacagga actttggtaa acaacatcga
    46681 caaaatcatc aatgctggtg tgaagttgct tgtggcatta gttcaaaact taccagcaat
    46741 catttctgcc attgtcaaag cagtaccgca aatcatcaca gggcttgcga atgctttcgg
    46801 tgagtatgtt tcaactatgg gaactatcgg gaagaacttg attacaggat tgtggaatgg
    46861 tatcgctgat gctggaagtt ggttacgcaa caagatttca ggattctttg gcggtgtagt
    46921 agacaacatc aaggacttct ttggtattca ttcaccatca acactgtttc gtgatgaaat
    46981 cgggaagtac atggcacaag ggattggcgt aggttttgat aaggaaatga ctggtgtttc
    47041 taaaacgatg cagaaagcta ttccaacaaa actttatatg ccaagctttg atattgatac
    47101 cgatatccac gctgccgttg atagcacatt ctcgatgctt gatttaggtg ctaaggttga
    47161 tgccttaaca aacatcacga gtgaaatgtt gccaacgcta cttaaagcaa tggacattaa
    47221 agtcgtcttg aatgatggaa cattggtcgg caaacttgct ccagaaattg ataagagctt
    47281 ggcgacgttg aatcgtagac gtgtagcata ttagtgttgg attttcacat tttgactgta
    47341 tactataact aacgagtgtt tactgaagag atggtggtaa tcatatgttg aagcataaat
    47401 ttaaagcaaa gaaacctgca ctgataggat atattctact gcttgctatt ggattatttt
    47461 taacggcagt aagatggtat agcggcataa atcatggttt tgtattcatc aacaatcaga
    47521 ttaattctca catttcaaac ttcgcattaa gctttctttt ctatttggtg ataggctatc
    47581 tttggttgct tatgggaacg aatttaaggg ttcttactgc gatgggagct ggtctgattg
    47641 ttgcaaatat tgtatgtgaa acgatgatga cttttctgaa tacaccagat ctaatcgatg
    47701 caatatatgg aatttccggc gtgctggttg tatacgcgta tttagcgatt accaagaaaa
    47761 tagggctgga agaaacaacc gaataggatg atagattata aactttagga ttagacattg
    47821 ttgtctggtc cttttttagt tatttagatg ggcggtggtc aattgaacgc atttatttta
    47881 gacggaacaa ttaattcacg gactgattta ggtttgcgaa ttacggaagt gccagttatt
    47941 ccagtgacgg aacgtgtcat tgatagtatt gagattgatg gacgtgaagg agatttgacc
    48001 attctgaaag gctggaaaga tgtgactttc ggttttaagg tagtgatttg gaaatctgat
    48061 gtttggacta gctggcagat aattttgcca caaatcctaa acgctaaaac tatcgcattt
    48121 tcaaatgaac caaacgtgtt ttacccaatc aagtatgcta aggcgagcgg actaactcag
    48181 attttatctt ccatgtggga gtttgaaatg gagttaacct gttcgccttt tcgttataag
    48241 accaatgtag cgactatcaa tcgaaccacg tcaggaacgg tcaataatcc cggcaacatt
    48301 tactcattgc cgaagattaa ggtctacggc aatggaacta ggacactcac gataaatggc
    48361 aagcctatcg tgttaaatct gacgaatggc aacctgacac tagattcgga tttgaaagaa
    48421 tgcttttacg gggatgtggc agccaatcaa tacatgacag gtgactttcc tgagtttcga
    48481 gtcggcagta atactgtgac tcttggtaca ggtataacca aagtagaaat agaaccgagg
    48541 tggcgatact tatgattacg ctttacaaca aaacagaatc aaatttcaat cataacggtc
    48601 tcgctgtcct tgatggcaac attctgaacc ccgtggtgag tgaggaattg aacggactct
    48661 tttcctttga attcgattat ccaattcatg ctccacacag tgaggaattg ttgccagaaa
    48721 tgattgtgaa agcaccagta ccagaactgt ccgaccagct tttcaggatt gttgaacgag
    48781 atgatgcact tggtggtttt ttgcacatag tagctcatca tattttctat gacctagcca
    48841 caaactttat tgaagatact tatatcgtca acaagaacgg gtcgggagct ttgacacaac
    48901 tgcttggtgc aactcaattc atgcaccgtt ttaccggtac atcaaacatc agtgcggtca
    48961 ataatgtccg cttggtgcga ctaaatcctg tagaggtgtt gcttgatagt gacctagaga
    49021 atggttttca aagcagttat ggtggtgaaa ttgtacgcga taacttcacg atttcaatgt
    49081 taacgactcg tggcagtaac aacggcgtac aaatccgaga caagaagaat ctgacaggat
    49141 ataaagctga tgttgattac tctggaattg ttactcgcat tatgccacaa gggtatgatg
    49201 gactatttct tcccgaaaaa tacgtggaca gtccaaacat caaccaatac gtccagccta
    49261 aaatcaaagt catcaagtac gacaacgtca aagttggtga tgaagaggat gagtttactc
    49321 aagccgaggc ctatgatgaa cttcgcagat tggctgggtt agaatactca gctaacaagg
    49381 tcgatttgcc aaaagccacc tatgatgtgg agtttgcccc gcttgagcga actgaagaat
    49441 ataaagattt tgcacagctt gaaacgatta atttgggtga tactgtttca gttattcatg
    49501 ccgaagatgg attgaacatc acagctagaa tggtctcata caaatacgat ccatttctga
    49561 aagcgtatat ctcagtaaca ctcggaaaca tcacgccaaa attcacggat gtggcgaagg
    49621 acatcaaccg ggtggacaac aaggcggagc aagccaaaga cgatgccaat tacgcattga
    49681 gttcggcaaa tggtaaaaac accaacttct atggcccgac aactccaaat aatccgaaac
    49741 tcggtgacgt ttggtacaaa gaaaatggtg acaagtttga aatgtgggtg tatgaaatcc
    49801 gtgatggcgt cactcagtgg tatccactta tgacagatat gacggcagaa gaagtaaagc
    49861 aagccgtgga acaagcacaa aaggatgcca atactgccac tgaaaaggcc aatcaagcat
    49921 ttaacgatgc tgtgaatgct ttgaatgcag ctgaaagtgt aggtgcaagt gttgaaacgc
    49981 ttactacatc cgttgaacag aatactagca agatttcatt agtggcgagt ggtatgtcaa
    50041 ctcttgaagg acgagtaatc acagctgaat caactctgac agtgcaagct gggcagattg
    50101 ctagtaaggc aagtcaatcg acggttgata atctgacagg gcgtattaca actgcagaaa
    50161 gcaccatcac ccaaaatgct gatagatttg agctatctct ttctaaaaca aataaagcgg
    50221 tcaatgtttt agccggtatg agcattgcag atttgcagtg ggcagttggt ggtctgaata
    50281 caaatgatgg aaaagaaaat acctatggtg gatacgttcg ctcaggtttt acaagagtta
    50341 gaaatggcga aaagtatcta cttcaaacat ttgtgggtgg gaaactttac tctacttacg
    50401 caacggctta tctgctttat tacaaagagg ataagtcctt tttatcgtac acccaatttg
    50461 gaaacaccac cacaccgctt actgttccaa gcagtgccgc ttttattcgc attcgtttaa
    50521 caactagtga tgcaccagaa accatcagtt gctatatgtt gaaaacggat gtgacaaatg
    50581 gctatgttga tttatccgag ataagtaacg tcgtaaaact tacagcgacc actgatgcct
    50641 tactgttaag tgtttcagaa accaaagaag caatcggcgt tccatttaaa gtcaatcaat
    50701 gggaacaagg aagtttgagt accactgacg gttcagaagt gtccgcaacc aattatctcc
    50761 gaagtgagtt ccttgatgtc aaagtgggag ataagtatat cgggcaactg cgtgacggaa
    50821 ctcaagctgc tttctactat cattactacg gcatggattt accttacatt gattttgtcc
    50881 caacggcaaa acaatttagt gaaaccctaa cggcgggaac ttatactaat gcgaccattg
    50941 cgagttatct agtaagtaag aacgttgaat cgatgagtat tacaagtggc gtgacaacaa
    51001 acccctattc aaattcagga gattatctgg tcatttacaa attgccattg gaaggcaaag
    51061 tcgctccgag tgtaatttcg tggaatggtc gcaaggatac atcagataat attgtcttgc
    51121 tttatacagg tggtgcttgg gaacagattt cgaccgttac aagtagcagc aatgccactt
    51181 attcttggac gttaacggct gagcagatat cagggattac tactgacagc gtgtatctag
    51241 ctttcttttc agttaagact ggcacatatg ctgggattta taattcaagc aacacacctt
    51301 ttactctaaa cccgtcggat ggttcaagtt atcatcaaat ctcttatact ggttcgagcg
    51361 gagcaattac agtcccgagt ggcacattga aaatgcgagt ccgagtgaac acgagcatca
    51421 aacccgatga atatgaagga aatatttatc ctacgaccac aaggcaagac tataccaatg
    51481 ccaacacatt atattcagca attctcatgc agaaagactt aatcaattta cgagttggta
    51541 aaggagacgt gattaatcaa atcaatatct cacctgaaag tattttgatt gctggaaaca
    51601 aggtgcacat cactggtcaa acaaccattg atagtgcagt gattagttct gccatgatcc
    51661 aaaatgcggc catcacaaac gcaaagattg cagatgccac aattcaaagt gcaaaaattg
    51721 caagcattga tgcggcgaag attaccacag gaactttgtc ggcagcccgt attggcgcga
    51781 actcgattac ggctgataag attgctgcta atatcctaac agcattgact gcgagttcgt
    51841 ccattcgaat cacaggaact acgattggat attattcagg aagcactctt gtgactgaaa
    51901 taaactcgca gggaatgacc attcgacgtg gtggaacgac agtcggaaca attggtgcaa
    51961 ataacatcag tggtcactct gattggcgtg gattggtatt tgatcttgag tatggaacag
    52021 agtatatgtc atgggctcat aaagattcct cctctgcgag tgtctacacc cagaagcttt
    52081 cttggtatgc gagaacattg cagtctggta tggagaaggg gttccacttt gacgacgaca
    52141 tttatttacc atcaatgctt cgtataggga cacaacatca aatatctgtt ggtgggatta
    52201 catttagtag ttccctttat actaagattg gctcaagttc cggcagcgct ggtattgcat
    52261 tcaacggttc taatctgatg ctcggagatg atgggaagtg ggttgacttt ggagtcatcc
    52321 gtgaaatctg tgccaaactt gcgggacgaa caatcgcttt accaaccggt ttcagtagca
    52381 acggtacagc gagtggttgg tacaatccgc agtcgtttaa ttcaatgacc acatacagtt
    52441 aggaggccgc catgaacgaa gaaaaagaag aagtgatagt aactgaacct atcgaggcag
    52501 atattccaaa gccaatgaaa gttgggacgg atatggttga tttgcttgtg atggtcgtgg
    52561 atgatctttg tgtccagatt ggtgatttaa aggagaagat agataatgct caaaattaga
    52621 aatgaagatt tggtggacgt gatgagtttc ttaaaaggtt ttgacttacc accaaagatt
    52681 agccgagtgc gaacgaagct tgcaaaactc atccaggtga aggttgatga actctatcaa
    52741 gacgaggtag acttgcttca aaaatacgga aagaaagatg agcacggcaa tttggtccaa
    52801 catgacggtg ccttttcact catcccagaa accgcaactg aatatcacaa agaaaaggca
    52861 gacttgctca acgaagaaac ccacatcaac gtggatgaac ttcaagacaa gctgccttta
    52921 ttggttgctg gatttgaaaa tagtgaagtc accatttcag ggaaagatgc tgaaatgctc
    52981 gatttaattt tagaacaatt ggaggatttg taaatgaaag aagtgtggaa ttggattcaa
    53041 ctcgccatcg caggtggtgg aggagtgatt ggatggtatg tgggaggaat tgatggtttg
    53101 atttatacgc ttgtaacttt tgtggtagtg gactatgtaa ctggtctgct tcgtgcaatc
    53161 aacgaaaaga aactatccag caagattggg gcaaagggca tcactaagaa aatcatgatt
    53221 tttcttttgg ttggtgtggc tcatatgctt gatgtggaag tcatcaaagg tggaaatgta
    53281 ctgcgtgatg cggtcatttt cttctatatc tcaaacgaag gaatttcact gctggagaat
    53341 gccgtggcga ttggactgcc agtgccggaa aagttgcgtg aaatcttgca gcagttacac
    53401 gaaaagaagg aggaaacaga aaatgggaat tagtaatcta gcaacaaaat atggatttca
    53461 gtcatttagc cattactcga atggtcgtag tggtgccaaa gtagataaga tagtgattca
    53521 tcacatggca accaccaact atgatattgt tcctgatatt tggaaaacgc gtgaggcgtc
    53581 agcacactac ggtattggaa aaaatggaga aattcgagca tacgtggacg aaggaaacac
    53641 agcttggcac gctggtaatt ggaacgccaa tatctcaagt atcgggattg agcactgcaa
    53701 tagcactgga tcaccgaact ggggtatcaa tcaagcaact attgatgcaa gtgcaaagtt
    53761 gtgtgcagat attgccaaac gttatggtct agggaaattg gtggtgaaca agaatatctt
    53821 ccctcactca catttttcgg ccacatcatg tccgggacag ttgcttggta aactgcagga
    53881 gattgcggat aaagccaatg cgattaataa tggttcatca accccaagca cgccagctag
    53941 tggtctgtat cgtgttcgca aatcatgggc tgacgccaag tcacaaattg gtgcctataa
    54001 ggacttgaac aatgcgaagc gggctgcaga tagccattct ggttataagg tgttcgacca
    54061 gaacggtaat caagtttatc catctgggtc atcaacatcc aacgtgaaaa ctcttgtggt
    54121 tcaagtgagt ggtctcaacg ttcgcaacaa gccaagttta agtggtgcgg ttgttggttc
    54181 atacaataaa ggcgcaactt ggacgcttcc gaagtcagag aagatgacga ttgcggatgg
    54241 ttgggtatgg gctcgttgcc cgattggata ttgtgctgtc ggtaagaata ctggtaagac
    54301 ggaatctgac gattatattt tgatttcata aatttaatcg gaacaaaatt atgtccggat
    54361 tgttgactta aaatatcgaa cgtgttatta ttaattcaac cagaacataa atttgttccg
    54421 attgaattat agaaagaggt atcgatatgg ttacgtttaa agatttgtta ccagaaccaa
    54481 cagaaagcca acaagctttc attttagatc acgggaaaac agaagatgat ggtcagttga
    54541 aatacgctga tgatgctaaa agttacggtt ggaacatgcg ccaatatggt aaattgaaag
    54601 ccggcgcggt tgttttgaat cgtcatccgg ggaaaattac taaagaccgt aagtgggaaa
    54661 tttacggggg cggatacgtt gagagcattt ctgaacctga tgaagctgga aatgttacgg
    54721 cagtaattac acacgcattt acaatcgaac cggcaattaa acaaggtgat tcatttattg
    54781 aaaacttcga ttggaataca cctagcaaaa agaagcgtaa aaagccgaat tcatgggcct
    54841 acttctggga tcagtatgga atgaatgaaa ttacctatgc cgattttgtt gcattgattg
    54901 aaaatcgtca tcttatccca atcgatgata cacaatcgtt gccgtctgaa ctggatctta
    54961 caaacgagga agttgaagaa attgagcaag aaagttcaaa aggatttact gttcttgttg
    55021 atgaagtggg accaaatcgc acgaatggga ctcagaaacg aaaattcact ggacgccata
    55081 ctgattggga gcgtgtcaac aaggcaaaac aaaaaacggg tgctctaggc gaagaaatcg
    55141 tacttgattt cctcattcag gaagcagaaa agaacaaaac aaaacttcct gaacatgttt
    55201 ctaaaactga aggagatggt catggctatg atatttgtgc gtttgaccaa tcaggtaatg
    55261 aaatccatat tgaagtgaaa gcttcaaaaa caaacttctc tgacggattt gaaatgagta
    55321 ctaatgaggt tgctgcctcc cttggagata caccatataa aatttacttt gttcacgacc
    55381 ttgatgtggc gtcaaaagtt tgcaaaatca aaatttatga tggcccaatt actgaagaaa
    55441 acttcacgat ggttccaacg agctacaaaa ttttcaataa gtagtttgac gtctttataa
    55501 ttcaaaacag cctcacaagt tttattgtgg ggcctttttt tcgtctagtt ttctttaagg
    55561 taggtgttta cagcattttg cattaggatt aaatgatttc ttaactgaaa tacctgctct
    55621 ttgtaattga tgcagaaaag aggcgattct attgattcca tacgattatt catcgcgttt
    55681 atattggctt gtaaaaactg gtataaagct tcttcagcct ctatggtttt aaagcacttt
    55741 tcaaattctt gtttgaagct ttcatcaaat tcctccagtt cctttgttgt cgcagatttg
    55801 caaaaatgtt catattcttt ttctaaccct tccatatcaa aactactcat aactttctcc
    55861 ttcccacaat tgatatttat attgttattt attggatgaa gcttgtaaag gcttatttga
    55921 tgtttgggaa gtaaaaagtc aaaatttaca aagaataatt cctactatat ataaacaaac
    55981 ttagaagcct gcaagagtga ttcgtttcat tcttgcaggc ttttttcgtt ttgtgggttc
    56041 gagtcggtta tttctttcgc atatggatga aaggcaaagg aaaatttgtg attgggttta
    56101 ctttcatgga ttttcttcgc ctgtatcttg aggaggcgaa agcaatgact aacaagaaaa
    56161 cagaaattaa ctattcagat gaagcagcct attacatttc acagcagatc cttctgaact
    56221 tacttgttac cggtaaaatc aacctggaag aatacgtcaa aatcgacaat aaaaaccggg
    56281 aaacattcaa tcctttttta tcaaggataa tggtctgaaa cgttgatata acggcacttt
    56341 agagctaaca tgtcacctaa tggaaaggag ttgagctgat gaaaacgata cataaattgg
    56401 cacgtccaga gttgccggaa gcttccaaac taaaggtggc agcatacgct cgtgtatcaa
    56461 catcaagtaa tgaacagctg gcaagtttac aaacccagat tactcactat gaaaatcaca
    56521 tcaagaacca tgaacaatgg gactatgtcg gtgtttatta tgatgaaggg atttctggaa
    56581 ccaagactgt aaaacgtgat ggtttaaaac gattgattaa ggatgcagag cttggaaaga
    56641 ttgatttgat tctgacaaag tctatttcca gattttcaag aaatacagtt gactgcttga
    56701 atctggttcg gcagctaact gatattggtg tcaccatctt ttttgaaaag gaaaacatta
    56761 atactggtga tatggaaagc gagttgttgc tttcaatttt atcaagcttg gcagaaagtg
    56821 aatcctattc ccattcagaa aacttgaagt gggcgattcg aaaccggatg tctaaaggga
    56881 aattcaaagc ttacccgccg tatggctaca agcggaaagg ctatgatttt cacatcgttg
    56941 cagctgaagc aaaagttgtt aaacaaatgt tccagtgggc attagatggc atttcgactg
    57001 gaatgattgc caagcggtta aacgatggaa aacaattcac taaacgtggg gcaaattggc
    57061 aagcaacatc agtcagaagt attttgaaaa atgaagttta taccgggact ttgacgatgc
    57121 aacaaacctt tgttgatgac cagtacaaac gacgcataaa caagggcgaa ttgccaatgt
    57181 accagatcaa aaacaatcac gaacccatag tatctaaaga agattacgac cgagttcaag
    57241 aactcattcg gagaaaagct gaggccaaag ggaatactgg tgacgggaaa aagtacacca
    57301 agcgatacgc attttcaggc aacatcatct gtgataaatg cggcagcaaa ttcaagcggg
    57361 ttcacactta ttggaaaggt aatacgaaga aggttcagtg gaagtgtacg gggcatctta
    57421 aaaataaaga tagctgcgat gcattaccaa ttacggatga agatttgaag gttgcttatt
    57481 tgaccatgtt gaataagttg attttcggac acaagttggt tctggaacct tttatgaagt
    57541 ctttgaataa agcaggtgcg aatcaggaag ggctcgaagt gcttgctgat gaaatcgaca
    57601 agattcaaga aaagcttgag gttctggcaa gtttgactgc ttccagtatt ttatcaacga
    57661 aggtgtcttt agaagaacaa agcaaacttc aacaagagag ggctgtcgca caagaaaagc
    57721 agcgcctcat cattgagtca gtcaacggca aaagcactca gcaaattgaa ctagaagcct
    57781 tatacaaatt cgcgcggatg gcagagatgt tgacggactg ggatgaagat ttattcaaga
    57841 agttcacaaa cagcattaca gtttacaacc gccaagaagt cggatttgaa cttaagtgcg
    57901 gactactttt gaaagagagg ttaccagaat gaacaataca attccatacg ggtatgtcat
    57961 taagaatgga gaaatcaacg tggatgaaca gcaagctcaa caaatacgtg agttcttcaa
    58021 tatttacctt gagggtgttg gcttacaaga agctggcaaa cgctctggca ttaacaagcc
    58081 gcatcccttt atgaaacgta ttttgatgaa tgaggtttac ttaggaaatg agatatatcc
    58141 aaaaatcatt gatgaggata ttttcttcca agcggatctg gagcaagaac gaagaattaa
    58201 gttctttaaa aaacgcaagg agatcaaagt gccagatacg aaggtaatac ccactcattt
    58261 taaatctggc aagattaccg aaaagtattc tgacccattc aaacaagcgc aatatgcata
    58321 ttcaaaaatc aaggaggtta agtgaaatgg caggaaaaca ggtgacggtt attccaatga
    58381 agcctagaca atgggcagca gagaataccg aagagaaacc aaagttgaaa gtggcagcct
    58441 attgtcgtgt ttctactgaa atggaggaac aggcgagttc ttacgaagca caggttcaac
    58501 actatacgga ctacattcaa agaaatcccg attgggagct tgtaggaata tttgcggatg
    58561 aagggatttc aggaactggg acgaaaaagc gtgacggttt taaccggatg attgaggcgt
    58621 gtcaaaacgg cgaagttgag tacatcatca caaaatcaat ttcacgcttt gcccgtaata
    58681 cggttgattg cttgcaatac atacgacagt taaaagaact gaagattgca gtgttttttg
    58741 aaaaggaaaa catcaataca atggatgcga agggtgaggt acttttgacc attatggcaa
    58801 gtctggccca gcaagaaagc cagtcactaa gtcaaaacac caagatgggc gtacagtatc
    58861 gtttccagca gggacagcta cgtatcaacc acaatcattt tcttgggtac actaaggacg
    58921 aagatggtaa cttggtgatt gaaccaaagg aagcagaagt catcaaacgc atcttccgcg
    58981 aataccttga gggcagttcc ttgcaagaaa ttgctaatgg tctaatggct gatggcatcc
    59041 taacgggagg gaagcgaaag ctctggcgag gagaaggagt cagattgatt cttcgaaacg
    59101 agaagtacat gggggatgcg ctgcttcaaa aaacatatac taccgatttc ttgacaaaga
    59161 aacgagtgaa gaatgacggg agttacgctc agcagtatta tgttgaagat agccatccgg
    59221 ccattatccc aaaagatatc ttcatgcaag cccagcaaga actagaccgg cgtaaatcaa
    59281 tgaaaaacaa aaagtcacag tgcttttctg ggaagtatgc gttatctggt atcacagttt
    59341 gcggtgactg tggcaatgct taccgcagag ttcattggaa gaatcgagga actgtgtggc
    59401 gatgcaaatc cagagtggat aaacgagaac ataattgcac gggtcgaaca atctacgaaa
    59461 cagacctgca tggagctgtt atcaaggcaa tcaatgaaac gttaatcgat cgtgatgctt
    59521 ttcttcagca attaaatgac aatatcaact cagttatgac tgatggatta actgaccgat
    59581 tggcagaact agacagtcag ttgaaagagc ttgaagctga aattattagc ctggcagttg
    59641 gcggtcaggg ttacgatgaa ttggcaagcc aaatattttc actaagagat gaacgagatg
    59701 cggttgcaaa acaaatagca gcgaatacta atttgcaaca acgggttgat gagatggtag
    59761 tctttgtaaa agaacacgat gtcatcaatg agtataatga agtgcttgtt cgaaggttga
    59821 ttgagaaggt caccattttt gaaaagaaca ttgttgttga tttcaaaagt ggggtgagag
    59881 tgactgtaga aatttaattt gaatagatag gactagacgt taatgtgggt agccatatta
    59941 acgtctttat tgattggatt tcataaagtg attgatgata taatatatta tgtaaaaaat
    60001 aggctggggg cgaagttctg tgagccaatg gaataccatt gatgaattaa gggatatttt
    60061 tattcgtaaa tatacggatt ctgagttgag taatgaactt gaaaaggcct gcagcgaaga
    60121 atatgaattg atgcgtgatt ataatgggcg tcagatttta gagttattac agaatgtaga
    60181 cgatgcctat ggagatataa aatcagaaaa tgatgctagc aatgacgaag tgaaagttca
    60241 gataacatat aaaaataata tcttggaagt tgggaataca ggcacttcat tttcaaaaga
    60301 tacaattgaa agattgtgct taggaagagc gagtaataaa tcatcacaaa atatcggaaa
    60361 taaggggact ggttttcgtt ccttgctaaa cgatgctgag tgggtcgaac tgtattcggg
    60421 agaatttgct attcgattta gtgaagaatt cacaaaaaag ctttttgagc agtatgtatc
    60481 aagagattct gacaaattca gtgaattaat ttttgagcag aagaaaaatt ggaaaaaaga
    60541 agattatgat ttatgttttc caataatgaa ctgtcctcaa ccaataaaca aaattgattc
    60601 tggttttgat acattaattc gagtaaaact aaaagaaact aatttaagta aaggcacgag
    60661 tgtttctaaa caacttcagc agccatttta taagtcactg ttatttttac caaatataac
    60721 aaaaattgtt attgaaacaa ctgatgatga aaaaatatca gagaaaattg tggatggaaa
    60781 tgatgtattg atagaaaaaa gtgatgttac tggtcaatca ccgtctgaag aatattttgt
    60841 ttttgacaaa gaatctatga taggagataa agttgctaaa attgcactag ccgtttctaa
    60901 agattctgat tacgattact ccaaagaaaa actatattgc tactttccag tacgtaattt
    60961 tggtacacca attcatgcac ttataaatgc accgtttata acaaataatt cacgtgatga
    61021 cgtcccggat gatagtgaac aaataaataa gaaaatattt attgaggtgc tcaaatttat
    61081 ttctgaagta gcagaaaaat tatctaagcc tgaatatgaa gacatagcat taaaaatggt
    61141 aactcctttt aaagagaata aactatggga cttagatgta tttgacttaa tgaacgaatt
    61201 cttgggtatt ttatctaatg cccaaatact accgactgtc aattggcaat atatttctat
    61261 caaagataat ccaaaacttt tccttcaaga attccctgag gagctaatag gtgagacatt
    61321 ttccaccttg ttgcaattat tagaaacaaa cgaaaatgac caactagtac aagaacttgc
    61381 taattataat gaatacactg gcctgtcgtt tgttgtagat gaactatctg ataaaatcaa
    61441 cattctttca tcttcttggg aaaatagaat aaggacaaaa gtgttcttat ggtggagtga
    61501 gcaatataaa agttctccga ttgtccctaa gctattaaag gacaatagtg acaattggtt
    61561 gatgaaatca gataaggtat tcttaccgac cgatacaggt gttactgtgc ttccaaaaga
    61621 gctgtcttct tgggttagtt taagcatttt gaaacaaaca tatgttgatg agttaattta
    61681 tcagattaag acttcatcaa gttggaaaaa taaatgggaa gaaacagcta atgcatacaa
    61741 agctgaacgt acaggagata aacgtattct agatgcattc agtgaaaaat atcttgcaat
    61801 tgaattcact gagcaaagca gttcagattt gatcattgga acgattaatc gacaaattga
    61861 cactgttgaa aaatctatca gtttcattaa ttggttttat gataactaca atgaaaaact
    61921 atcagctgga agtgagctgt ctaaagttcc atttaatctt ccaaacgttg ctggtgacgt
    61981 tcaaccttcc aataaaatgt atattggaaa agagtatggc aatgaattag gagataagct
    62041 ttttcaagat acagattaca cggcattgaa aaaaatagat tgctctagtt ttgctgagga
    62101 gagagatttt ctgtcattta ttcttcgatg tggtgtttcc aaatatccta agatttatga
    62161 taacgattct ttaaaaaatg atgttggatt taaaaagttt atttataaca aatataagaa
    62221 tgaaataaaa tttacgaata ttaattattt aacttctaag tacatcagta attttgaaga
    62281 attaatagac aaattagata cgaaagacat tgtcaattgg ctcaatcaag acgaagaatt
    62341 gacagattta ttatcttcgg taaaaaaaga atcaagtgca agtcagcaat cgaactggag
    62401 tggattgtac tttaattcaa atgagtatgt gaaatacatt ttgaataaaa ctgcatggat
    62461 taagattgat ggagaaaaat atagcccgag acaaattgtg ctatatgaaa agctccaggc
    62521 acacgttccg ggattgtatg gagtttctga acaaagctta atggaactct tggggcaacg
    62581 cattgttgaa cagttggatt tcagaaaaaa tatggcgatg ttttcggatg atgaaataag
    62641 agctatatta gttgagttac caaaattcga taaaggcgaa ataagtcgca gattatataa
    62701 tgaacttata agaaataaaa aaggaatgaa tcctacttat tctaccgaag gattatcagt
    62761 cttagccaaa gatggcttct tttatccaaa caaagaatta aggtatgctg ataagcgact
    62821 gcctaaatca atagaaaaac aaatgcgttt tatttacgtg cctgaaaaag ctagtacaac
    62881 tacgatatat gaatggcttg gggttgaacg cttcaaaaca aatttaaaat tggaaacatt
    62941 tgagttattg gaagattatt ctgaggattt caaaaaagag attgatgata tcaaaactgc
    63001 ggtattatct acgatagatg acaatagtaa gaatgttagt tcattaaaac gtattcagat
    63061 tattccatgc agcaaaatat ctgctaaaga tgttgagcaa gatggtcagt caatcgtttt
    63121 ggaagattat tattttgtgg aaagtaaagg aaaattctac ttaaaattac ctgctaattc
    63181 tattgaaatt aatcaattac gtttgattga tacgttttct tcaacaatcg tagacatatt
    63241 caagcaggtg ttaaatctaa gcttagattt gaatttgatt gaattgttaa tttcccgaga
    63301 tacggaacat aaacaaagaa aaataactga agaattcggt gtagataagt ggaatgaatc
    63361 gtttgagcta ttgtttagcg aaaatgcgtt aaataaaaaa gttaatgact ttttgttatc
    63421 aaatggacta aatgaagagg aatatcctac catttttgaa attgactatt catatgcatt
    63481 aaaagaagaa gagtatcaat tgttggcgat agctctagaa agcatttcaa aagatattga
    63541 ggatttgaat ggattgtcag aattgattca tttggatatt cgtgaatttt ggaaactgaa
    63601 attaagatct tatctagata agaaagcgga acattaccgc cagattttat atagcaatgc
    63661 aaataaaagc ggtgatgaaa tactcaaaga aacattccta gaaaaaattg atagatacaa
    63721 agattatcaa tttgagattt ctgaaattcc aaattcagtt caagtcgaca ttgagaaaga
    63781 aagtaagaag agatttccag agctaagtgt gtcaattact gatgaaattg acattgatgg
    63841 cctgtatgat aataatgtcg aagagttagt cacggttgca gctattacaa tagatgattt
    63901 tgattattat attagaggtc atcaaaaagt ttcttcctta ctgtattttg gtattccgtc
    63961 ccgtttaaga gaagatatac tgatattcgt tactaaagaa aatgatgttg atttgaacgg
    64021 agaaaaatct ggtatctcta ttgaaggaac atcaactgtt caaaccattc ttgaaccatc
    64081 gaatagtgtt agtaggaaag gtaagaacac acgaaatgga gaaagaagta aacgagatta
    64141 tgataataga aactccgcaa atgatgatgc tggtgaaaca gcagaacaaa ttgcttacgc
    64201 tgaattgact cagaatcaaa aacttaatgt aatttggaat acgaaatatt ctagtaaatc
    64261 ttcagatcgt aataagcagc caccaaacgg aattgtttgt gatatgtggg ttcatgaccc
    64321 agagaatggg aatatgtact ttgaggtaaa atcatcaatt acagaatttg aaatgtcgat
    64381 taatgaatat aattctatgt tagataaccc tgactcttac gaggtcgttt tagtcaatcg
    64441 tgataccaaa gagataagtc gtcataaatt tgatgaactt gatggattga aacaggcaag
    64501 tagctatctt ttcaaattta agcaaaagag aagtgacggt tgagtgattt gtttccacaa
    64561 acctattcaa tctatcaact caaaaatcat aaacaaccat tattatagta ggaagataca
    64621 gagtgtactc tcaacgcata tcgaattagt cgcttcattc gttaaaggag aataaaaatg
    64681 gaccaaaaag aaaaaatgaa agaattgatc aaaaagaaac aaggtggagg ccgatccaaa
    64741 caaatggaaa cacctaagaa tgatcgtaaa aacatgagaa aaggccctaa aatctttaat
    64801 aaatagacgt gctaaagaaa gactaagaag tgttaacttt tgttattgtt tcttagtctt
    64861 ttta
//
LOCUS       DAZAID010000004        60463 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000004 DAZAID010000000
VERSION     DAZAID010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.004"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1420)
                     /locus_tag="ACG9BE_000203"
     CDS             complement(<1..1420)
                     /locus_tag="ACG9BE_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFN3030992.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
     gene            1672..2853
                     /locus_tag="ACG9BE_000204"
     CDS             1672..2853
                     /locus_tag="ACG9BE_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN3030993.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(2913..4541)
                     /gene="treC"
                     /locus_tag="ACG9BE_000205"
     CDS             complement(2913..4541)
                     /gene="treC"
                     /locus_tag="ACG9BE_000205"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFN3030994.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(4992..7703)
                     /locus_tag="ACG9BE_000206"
     CDS             complement(4992..7703)
                     /locus_tag="ACG9BE_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFN3030995.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7713..8414)
                     /locus_tag="ACG9BE_000207"
     CDS             complement(7713..8414)
                     /locus_tag="ACG9BE_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3030996.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8600..9199
                     /locus_tag="ACG9BE_000208"
     CDS             8600..9199
                     /locus_tag="ACG9BE_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFN3030997.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9280..10293
                     /locus_tag="ACG9BE_000209"
     CDS             9280..10293
                     /locus_tag="ACG9BE_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFN3030998.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10401..11318
                     /gene="rbsK"
                     /locus_tag="ACG9BE_000210"
     CDS             10401..11318
                     /gene="rbsK"
                     /locus_tag="ACG9BE_000210"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFN3030999.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11346..11741
                     /gene="rbsD"
                     /locus_tag="ACG9BE_000211"
     CDS             11346..11741
                     /gene="rbsD"
                     /locus_tag="ACG9BE_000211"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFN3031000.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11741..13114
                     /locus_tag="ACG9BE_000212"
     CDS             11741..13114
                     /locus_tag="ACG9BE_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3031001.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13471..15615
                     /locus_tag="ACG9BE_000213"
     CDS             13471..15615
                     /locus_tag="ACG9BE_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN3031002.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(15667..16707)
                     /locus_tag="ACG9BE_000214"
     CDS             complement(15667..16707)
                     /locus_tag="ACG9BE_000214"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN3031003.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(16984..18786)
                     /locus_tag="ACG9BE_000215"
     CDS             complement(16984..18786)
                     /locus_tag="ACG9BE_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3031004.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18800..19552)
                     /locus_tag="ACG9BE_000216"
     CDS             complement(18800..19552)
                     /locus_tag="ACG9BE_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3031005.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19561..19923)
                     /locus_tag="ACG9BE_000217"
     CDS             complement(19561..19923)
                     /locus_tag="ACG9BE_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN3031006.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20099..20971)
                     /locus_tag="ACG9BE_000218"
     CDS             complement(20099..20971)
                     /locus_tag="ACG9BE_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFN3031007.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21022..21510)
                     /locus_tag="ACG9BE_000219"
     CDS             complement(21022..21510)
                     /locus_tag="ACG9BE_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFN3031008.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(21648..22439)
                     /locus_tag="ACG9BE_000220"
     CDS             complement(21648..22439)
                     /locus_tag="ACG9BE_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFN3031009.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22494..23102)
                     /locus_tag="ACG9BE_000221"
     CDS             complement(22494..23102)
                     /locus_tag="ACG9BE_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFN3031010.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23095..23412)
                     /locus_tag="ACG9BE_000222"
     CDS             complement(23095..23412)
                     /locus_tag="ACG9BE_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN3031011.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23568..24296)
                     /locus_tag="ACG9BE_000223"
     CDS             complement(23568..24296)
                     /locus_tag="ACG9BE_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFN3031012.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24455..25336
                     /locus_tag="ACG9BE_000224"
     CDS             24455..25336
                     /locus_tag="ACG9BE_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN3031013.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25573..26616)
                     /locus_tag="ACG9BE_000225"
     CDS             complement(25573..26616)
                     /locus_tag="ACG9BE_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3031014.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26619..27287)
                     /locus_tag="ACG9BE_000226"
     CDS             complement(26619..27287)
                     /locus_tag="ACG9BE_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3031015.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27429..29765
                     /locus_tag="ACG9BE_000227"
     CDS             27429..29765
                     /locus_tag="ACG9BE_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFN3031016.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29880..30191)
                     /locus_tag="ACG9BE_000228"
     CDS             complement(29880..30191)
                     /locus_tag="ACG9BE_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031017.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30372..31847
                     /locus_tag="ACG9BE_000229"
     CDS             30372..31847
                     /locus_tag="ACG9BE_000229"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFN3031018.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31894..32478
                     /locus_tag="ACG9BE_000230"
     CDS             31894..32478
                     /locus_tag="ACG9BE_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFN3031019.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            32480..32863
                     /locus_tag="ACG9BE_000231"
     CDS             32480..32863
                     /locus_tag="ACG9BE_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFN3031020.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(33180..33464)
                     /locus_tag="ACG9BE_000232"
     CDS             complement(33180..33464)
                     /locus_tag="ACG9BE_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFN3031021.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33816..34526)
                     /locus_tag="ACG9BE_000233"
     CDS             complement(33816..34526)
                     /locus_tag="ACG9BE_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFN3031022.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34523..35995)
                     /locus_tag="ACG9BE_000234"
     CDS             complement(34523..35995)
                     /locus_tag="ACG9BE_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFN3031023.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36133..36606
                     /locus_tag="ACG9BE_000235"
     CDS             36133..36606
                     /locus_tag="ACG9BE_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN3031024.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36759..37310)
                     /locus_tag="ACG9BE_000236"
     CDS             complement(36759..37310)
                     /locus_tag="ACG9BE_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN3031025.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37415..37945
                     /locus_tag="ACG9BE_000237"
     CDS             37415..37945
                     /locus_tag="ACG9BE_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFN3031026.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38428..38871)
                     /locus_tag="ACG9BE_000238"
     CDS             complement(38428..38871)
                     /locus_tag="ACG9BE_000238"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3031027.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38887..40047)
                     /locus_tag="ACG9BE_000239"
     CDS             complement(38887..40047)
                     /locus_tag="ACG9BE_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN3031028.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40554..42254
                     /locus_tag="ACG9BE_000240"
     CDS             40554..42254
                     /locus_tag="ACG9BE_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031029.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42356..43189)
                     /locus_tag="ACG9BE_000241"
     CDS             complement(42356..43189)
                     /locus_tag="ACG9BE_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFN3031030.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43493..45658)
                     /locus_tag="ACG9BE_000242"
     CDS             complement(43493..45658)
                     /locus_tag="ACG9BE_000242"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFN3031031.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45918..48212
                     /locus_tag="ACG9BE_000243"
     CDS             45918..48212
                     /locus_tag="ACG9BE_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFN3031032.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48205..48870
                     /gene="pgmB"
                     /locus_tag="ACG9BE_000244"
     CDS             48205..48870
                     /gene="pgmB"
                     /locus_tag="ACG9BE_000244"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFN3031033.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            48863..49870
                     /locus_tag="ACG9BE_000245"
     CDS             48863..49870
                     /locus_tag="ACG9BE_000245"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN3031034.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49892..50911
                     /locus_tag="ACG9BE_000246"
     CDS             49892..50911
                     /locus_tag="ACG9BE_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3031035.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51192..52559)
                     /gene="nhaC"
                     /locus_tag="ACG9BE_000247"
     CDS             complement(51192..52559)
                     /gene="nhaC"
                     /locus_tag="ACG9BE_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFN3031036.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52668..54089)
                     /gene="tyrP"
                     /locus_tag="ACG9BE_000248"
     CDS             complement(52668..54089)
                     /gene="tyrP"
                     /locus_tag="ACG9BE_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFN3031037.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54332..56209)
                     /gene="tdc"
                     /locus_tag="ACG9BE_000249"
     CDS             complement(54332..56209)
                     /gene="tdc"
                     /locus_tag="ACG9BE_000249"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFN3031038.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56500..57756)
                     /gene="tyrS"
                     /locus_tag="ACG9BE_000250"
     CDS             complement(56500..57756)
                     /gene="tyrS"
                     /locus_tag="ACG9BE_000250"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFN3031039.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57868..58073)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58336..59637
                     /locus_tag="ACG9BE_000251"
     CDS             58336..59637
                     /locus_tag="ACG9BE_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN3031040.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59706..60305)
                     /locus_tag="ACG9BE_000252"
     CDS             complement(59706..60305)
                     /locus_tag="ACG9BE_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3031041.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        18263 a        12361 c        10832 g        19007 t
ORIGIN      
        1 ttcctgcata taaatgaaaa tgttcggagt cagccggtgc aatcgtgtga tcactgaatt
       61 gaatatatct aaaagcgcca ctttccttat cttctgcttc gaatagatac cttacgccac
      121 ggtttcctgc ctcgtaggtc aagatttctt tccctacata acgataattt gaacgtttcg
      181 ttgttccatt cttaaacgta aatgccatct ctttctcact gatcttgatt gaagcaatat
      241 ctgtttgata gcccttcgta taatattctt tatattcttg tgctgtctta tcctgattca
      301 atttttcttt atattcaaaa acaggatcaa aagtcccatc ttctacatac gtatctactg
      361 tttgccaagt tcctgcccaa tctgaaagag agcgatcttg tacatcctcg tcttcaaaat
      421 aaccgttgta gacgctcttt tcttttttgg gggtcaaagt atcttctagc gggttttctg
      481 tctctgtcgt ttttttcaac gcttcgaggt ttgcttccat gatagaaaga tagttttctc
      541 cattttctat ttgttcattc gtcaatcctt ccaaaggatt caatacttct aaagatacac
      601 ctgcttcgtt tgctaacgtt ctggcaatac tatctttggc gttttcttca aaataaatat
      661 agttgattcc atgttcttta acaaagtgtt tcagttcagc aattctcgcc gcagacggtt
      721 cttcactaga agaaagacca gcaatcgata cttgattcaa tccatattct aacgctagat
      781 agttgaatgc agcatgttgc gtgacaaaag atctttgttt tgcttgctta aaggtttctt
      841 gataaagctg atggagtgct tgaagtttct cggtataggc tgctgcattt tttgtccata
      901 cttcctgttt ttctgggtaa gcttcaatca gctgatcacg tatgtttgct acctgcttga
      961 ttgctaaact tggagctagc catacatgtg ggtctagctc atgtgtatgg ccttcttccc
     1021 cgtgttcatg gtcatgatca tgtccttctt cacttcccgg aagtaaaagc ataccttctg
     1081 tcgcttttat cgtatggaca ttgccttctt gtaatgtttt ttggattgcg gggacccatg
     1141 tttccatgtt ttcatcatta taaacaaaga tatctgtatc ttggatcttt gccatgtctt
     1201 tagcagaagg ttcataatcg tgggaatcag tccctgctgg aatcaataac tctacttctc
     1261 cttcatcccc gacgattgct tttgtaaaat catacatggg ataaaaggtc gtcatcacgc
     1321 tcaatttttc tttactgtcc tcggctttcc cacttgcccc gcaagcagtt aacatacctg
     1381 ctaaaaccag catggccaat atacctatca atcgtttcat ttatgacact cctcttctca
     1441 tcttctttca tttgaatgtt ccgattcgct tttcgcaaac cgtaatagtt acgttttagc
     1501 aacaggattt aatttatcat atggttaaaa gaactgtcaa gcgttttctt attttaattg
     1561 atgagaaagt ttatatgaat gattgacgat atttttgcaa tcaacctcta tcattttcct
     1621 tttatacccg cttttgtcta taatgaagtt atagataaag gaggaaaaag agtgaaaaaa
     1681 gaaacagata caacgaatcg tttgatccgc tttttaggag gaaaaacctc ttactatgtt
     1741 ttaggaatag ttatcttact tgcgctgatc atttattttt tccatcagat ctcgtttata
     1801 ttcaagcctg tcggaatcat tatcgctact atcttgcccc cattattgtt tgcactgatc
     1861 ttgtattatc ttttggaacc agtcatcaaa cgtttgagca agcacctttc gaggaacttg
     1921 tcagtcatta tcgtttatgt gattatttta tttttattgg ggcttggaag catatggctt
     1981 attccttttc ttgaagaaca ggcaaagaca ttgatcagta gtttgccaga tttgtttcaa
     2041 gattttcaag agagtctccg acaatttttg gagaaaacac cttttgccga ttcatttaat
     2101 caatttttcg cttctattga cgacgtgacg tctgatatag ctggatttat tggaaattat
     2161 tgggaaagtg gcgcacaacg gatcagcaac attttttcaa ctgttacagc tatttttatt
     2221 actttattta ctggtccaat tgtcgctttt ttccttttga gagatcctca aaaattttat
     2281 cagtcagttt tagcgattgt cccaccagca tttcgtacag attttaaaaa tttgacaaag
     2341 attgctaatc aacaattggg ttcatttcta aaggggcaaa tcattgcttc atttatcctt
     2401 ggagcagtct actgggtttg tttcttactg atcggattag aatttgctag tgttttagct
     2461 atttctgctg ggttgttatg tatcattcca tatattgggc cgtttatcgc cttctttcca
     2521 ggtttgatca ttgcattcca agactctact tttatggctg tcaaatttgt cattgtctgg
     2581 tttgccgtac aacttctcca cggtgacttg gtgattcctc gagtaatggg tgatcgttta
     2641 cagatccatc cgattacgat cttaatcgtt ttacttgtca tgggtgatct aatgggaatt
     2701 gtgggcgtta tttttggtat tcctatctat acgttagtca aattactcgt tacttttatt
     2761 ttccgtaaat tcaaacaacg ctacaataaa ttttatggcg ataaaggaga gtatgaaaac
     2821 tcttcatttt ctgaagaaga ttattttaaa taaaacacaa aacgtcgatc ttacgagaat
     2881 agctcgtagg aacgacgttt tgtgtttttg ttttaatttt tcagcaaagc aattgcctgg
     2941 tatggtttta gctccagact ttgatgaaga acagtatcgg aataattatc gataagaata
     3001 ttggcttctg tgaatccttc tgggatggcg atccttgtat ctttatcata gaaatttgtt
     3061 aatacaagca gctgctgtcc ttcaaattgg cgaagaaagc cataaacttc cggatgattt
     3121 gcttcataag catgataatc tccttgtgca acgattggat atgtttttct cagccgaatc
     3181 aatttcttgt aataggacaa aatagaatct tcagaagata gttctttttc tacattaatc
     3241 tcttctagct tgcctccaac ttttaaccaa ggtttccctg tagtaaatcc tgcttgttct
     3301 gtgtctgacc attgcatggg tgttcgagag ttatcgcgag attttgcttt gatccgacga
     3361 aacgcttctt tttctgtgaa tccttgttca aggagtaact tataagcatt caaactttct
     3421 acgtcaatat agtcggaaat cgatgaatag tctgggtcga tcatgccgat ttcttctccc
     3481 atataaatat agggcgtccc acggttcaaa tggatcatgg ttgctagcat ctctgctcct
     3541 tgttttcgat actgatcgat agctacaaat cgattgagcg cacgtggctg atcatgatta
     3601 ttaaaaaata gagcattcca gccgttaccc tcactcatct tttctcccca tgtatgaaat
     3661 agtcgcttta attctgcaaa atcaaatgga ggagtagacc atttattccc attttcataa
     3721 tcgactttca aatgatggaa attaaagacc attgataatt cttctctact aggttctgta
     3781 tacaagatac aattttcgat cgtcgtagag ctcatttcac cgactgtgat gatagtggaa
     3841 tccgtcccaa acgttgcttg gttcaattct tttagatacg tatgggtgat tggtttgtcc
     3901 gtatattcag gtttgccatc attttcttta ttatttttca attgttcgtc tttaccgata
     3961 acattgataa catcgaaacg gaatcctttt accccttttt ccatccaaaa acgaacaact
     4021 ttgaatagtt cctctcttac tgcttggttc cgccagttca agtcagcttg tgtccgatca
     4081 tataagtgca aataatattt ccccgtatct ccaaatggcg accaagcact tccaccaaat
     4141 tttgataccc aatccgttgg ctcttctctt aaaatgaaga agtcctgata atgctggtcc
     4201 cctgctagtg cttttttgaa ccattcatgt tctgtcgaaa cgtgattcag caccatatcg
     4261 agcatgattt ctatccggta tttctttgct tcggctatta gctgttcgaa ttcctccatc
     4321 gtaccaaaga taggatcgat ggctgtgtaa tcagagatat cataaccgtt gtctttttga
     4381 gggcttggat aaatagggtt tagccaaatc atgtcgatgc ctagatcatt cagatacggg
     4441 agtttttctt tgattccttt taaatctcca attccatcac cattactgtc tttgtaagat
     4501 ttgggataga tttggtaaat gactttgtct ttaaatgtca tggatattta cctccattca
     4561 actgatttat tacctaccac aacttatata tacaagttat catttattat tataactgaa
     4621 ccgaaagcgt ttgtaaaatt tatctttata cattctaaga ggtctgttgc ccaataaaaa
     4681 acaagagatc agacatgtat ttgtctatcc tcttgtttaa aatagcaaaa tattcttgtt
     4741 tcttctataa atggtgttca aaagctttta tctacggcaa tcagtcttct caaatgtaaa
     4801 acatgaggag ctaattttat ttgagtattc tttttgctcg atgcaggacg gcttttttac
     4861 aacctctgat tcatggtgtt caatcagctg tccttcggta ttagctcaaa aaacggcaaa
     4921 atatgaaaaa ctattttgcc gtttgtttct ctaatactca ggtcaaaccg ctgatttcac
     4981 aacctctgtt tttattcatt cgatttcaac gcatcaatca tatcgatatg ctttaatttg
     5041 aaatgggtaa caatcatcac aataaccgta aatacgatag ttaatcctgc tgataagaca
     5101 taagcccacc atgtgatcac taatggaaag atgacattct ccatagacgc ttgttgcaag
     5161 atgaagtcag tcaatatata tcctacacca aatcctccga tgattcctaa aagagtaaag
     5221 atgatatttt ctcgaacaat gtacatcgtg acttctttat caaaaaatcc aaggactttt
     5281 attgtagata attcgcgtac acgctcggaa atattgatat tagttagatt atacaagacg
     5341 ataaaagcca gcagtcctga taatacgaca aagattaaga caaccgagtc taagttcgcc
     5401 attgattctt cttgcgtttc gatttgtttg gacatgaaag acgtatttgt cacagcgtct
     5461 gtggcaagca gtttctcgga aagcatttct tcttcttttg ttgtcatctt atctgtacgc
     5521 agcaaaaatg cattatccat catcgattga tcagtaactg aagcataata ttcaggggac
     5581 atataaataa agctacctag atagtttttc acgatttctt ttacttcgac tttccattct
     5641 ttttgatctt catcataaag cgaaatatga tctcctgtag ataagtcaaa tagttctgct
     5701 actttttgcg taatagcgat tcctgtttta ggcaaggagc gtgggttctc ttcaagatca
     5761 gttaaatgga taaaacgatc caaatcagct gttttacttg gtacgatcac tgtcagactt
     5821 tgcgaagcat gtcctttttt tctcaattct acggtttgag tatgaatagc taggatctca
     5881 tttacttttt cttgcttctg cagcatatcc gctgcttttt gacgactatc ttccgtggat
     5941 gaatccaatg taacgattgc ttgatagtca atgatcggtc caaattgttt ggctgaaaca
     6001 gaacttagag aatctttcaa tcccacgcct gcaaccatca gcccggcaca tccagcaatt
     6061 ccgataatcg ccataagcat tcttgactta tatcggaaga gatttcggta actgattttt
     6121 tgattaaagc taaggcgtga ccacagcgga gtgatgtatt caagaaaaat gcgtttccct
     6181 ggcttcggtg ctttcggttg aagcaatgca gcaggccgtt ctcttaaatc tttgaataaa
     6241 acgatcattg cagcgccaaa tgcggcaatg aagaatgcgg cagctgcttg taaaatcggc
     6301 ggccagtcat aaaaaataag cgcactccca atatcatacc gctcgttaga cagctcaaaa
     6361 atgatccttg gcaaaagatt cgttcctaat atcgttccta aaacaatacc tatccctgag
     6421 gctagacttg catagattgc atattttttt gcgatttcaa ctttcgtata acctaacgcc
     6481 tttaatgtcc cgatttccct gcggttttct tctaccatac gtgtcattgt cgtaaaggtg
     6541 atcaatgcgg caataaagaa aaagaaaaca ggaaaaacat tggcaatcgc agcgatacga
     6601 tcagacagat caccaaattc ttgaaaaccg acattttctg aacgttcatt gtataggtat
     6661 gatggtttag ttaaattttc aattttttct gcatttcgat tcaattcttc ctctgcagag
     6721 gccagttcac gttctttctc aactaattgc ctttcttgtt cagcaagttg agcttcttgt
     6781 tgagataatg tttgaacagc gattgttcgt tgcgcttcag gaagctgctg gatagttgct
     6841 gactgttgcg ctaactgttt tttagcatct tctaaccgtg ttcttgcttg cgttgtttgt
     6901 gctttgccat ccgctatttt ttgctgtgcc ggttctaatt cttcgttggc cgattttttc
     6961 agctcttcta agcgttcttc gggtcgatct tgtaaatact tttctgcctt ttcttgattt
     7021 tgctccattt tatctttgta agctttgctg tacgtattca aattctttac gtttgtaaaa
     7081 tcaagatata agacagaata aatatctgtt ttaaaaacgg attcaggtaa atagacaaaa
     7141 taatcgacgc taccatttcc gacgttcgcg tatcctctct ccaaattgtt gatgaacaat
     7201 ggagaacgga caaagcccgt gatggtatac tctttttctt tcaattcatc tgaatcaatc
     7261 tgataaatat caccgatttt atacccttca tcttccgctt tttcatccaa tacaagctgg
     7321 tttggttttt ccgggagttt tccatcagtt aattgtagct tgttctgctt gttatttttg
     7381 ttataagaaa atatttgaat cacttcgttt ttgccagtat tggaataata gatctgtttt
     7441 cctgtttcta cttttgcatc tgggatcttt tctgcttgag caatatcttt ttgtgtcaag
     7501 ccaccagtag aaacgatttg caaatcagaa agtcctgctg taccaacata gtgatccatc
     7561 gttttttgca tgacaggtcc agcagttttg acacctacaa ataaaagtgt tcctagcatg
     7621 acaataagga tgattgcaat gaatcgtcct ttagattgcc gaatttcgcg aaaacctgct
     7681 ttaagatatg ttcttgtttt cataaaatcc ccctaccata caatctcatc aatggatgaa
     7741 ggatgatcat ttaattcaac agaccttact ttagcatcat taatgtgaat gactctatca
     7801 gcgatgggcg ctaaagcaga attatgtgta atgatcaaaa cggtattgcc gtgttgtctg
     7861 ctagcgtttt gcaataattt caatacttgc ttgcccgttt caaaatccag cgcgcctgtc
     7921 ggttcatcac ataaaagaag tttgggattt ttagctagtg cacgagcaat tgaaaccctc
     7981 tgctgttcgc ctcctgataa ttgggacgga aagttattca atcgatgagc taggcccact
     8041 tgcctcaata cctctacagg atcaagcgca tcaggagaaa cttccgttgc caattccaca
     8101 ttttcttttg ctgttagatt aggaacgaga ttgtagaatt ggaaaacgaa ccctacatct
     8161 gttcgtcggt aagcagtaag ctgtttatct gaaaattgcg caatatctgt gtcatcaata
     8221 atgatctgac cttcatcagg tgtatccatc ccacctaaaa tattaagaat cgttgacttt
     8281 cctgctccac ttggaccaag tataatcact aattcgcctt tttcaattga aaaagaaata
     8341 tcgtcatttg ctgtaatggt cgtttctccc attttgtatc gtttgtattc attttttact
     8401 tcgacatact tcactctgat tcctccattt tcacgatatt tctcgctata tctatgcatc
     8461 gaatgcgttt tctcacactt atttatttta ccactgaatt tctctagact aaagaaagga
     8521 atcctttaat ttagttgaca aataaacgat ttatgattta cttacataaa aaacattgtt
     8581 atttgaaaga aggaacatca tgaatgattt aaaggaaaat atgatttttg atttatcaaa
     8641 aactgctttt gtcgcgatcg acatgcaaaa agagatcgta aataacggta cactctctcc
     8701 acacactgca gcatctattc tgacaaacaa tgatttgtta gtaagaacat tgaaacgtac
     8761 cgctgccttg aaagttttag tgaacgtaga catctccact ttcccttatt tgagtcaaca
     8821 aactgatatg ggtggaatga ctgcttctgt acctccagaa ttcactgatt tacttttgaa
     8881 ggattctttg aaagatacag ataatatgtt gactatcaca aaatacaatc caagtgcttt
     8941 ttttggtact tctcttgatc ttcaacttcg ccggcgtgga atcgaaacta tcattttaag
     9001 cggtgtagcg acaacaaacg gtgtatatgc tactgcatta gatgcctttc aacacgggta
     9061 ccatatcgtg cttgctgaag atgcttgcag tgatcgtgac aaagaatccc atcaattgtt
     9121 cataaaaaag atatttccga aaactgcacg agtacgctca acgaaacaaa taatcgaagc
     9181 tatccaacaa agcaaataag tactggcaca cgtgattttc atttacttct ttttgatttc
     9241 atgggaaaat aaccacagga tttagatagg agtatgaaca tggtgaaaaa aaagatcaca
     9301 atcaaagaag tcgctaaact ttccggcgtt tcaatcacaa ctgtctcctt gatattaaat
     9361 ggaaatacgg agaaattcag tccgaaaact gttcagaaag ttttagctgc caaagaagaa
     9421 ttaggctatc agcctgacta ttttgctaga caaatgatta ccaaggaaac gaaaacaatt
     9481 ggtgtactcg ttcctgatat cacgaatcca ttcttcagta cactgatgcg aggaattgag
     9541 gatatcttgt ataagcagaa tttcgtcacg atattatgta atgctgattc tgatcatcaa
     9601 aaagaaatcg aatatctagc cgagctcaca cgtcgaggtg tggatggatt cattatcgcg
     9661 acttctgctg tctctacaga tgcaatcaac gaaaatttga aaaaacaagg ccggcccttc
     9721 attgttttgg atcagaaaaa atctgaagga tttagtgatg ccgtacgaac agacgatttt
     9781 cgcggtggtt acctagcggg catgcacttg cttagtctag gacatcaaac gatcgccctt
     9841 gtctatcctg aaaatccacc ggaaaatgtc cacgctcgga ttgaggggtt taaatctgct
     9901 cttgatgttt accaaatccc tcacgaccaa ctgatcctct tacctacaca gttctcaaaa
     9961 caaggtggct accagatcac agcagaactt cttgattctg ctgcaactgg tgtgtttgct
    10021 ttaaatgatg aattagcttt tggattatat cgcggattag aggaagctgg aaaaagcatc
    10081 cctgaagact acagcattat tggctatgat aatatagata tgtgcgagta tatcaagccg
    10141 aaactgacaa cgatcgcaca accaatcttc gaattgggac aaacatctgc caagttattg
    10201 ttagatcgga tccaattccc agaaaaagaa tgggaagaaa aaagattacc tgtacgatta
    10261 gaaaaaagat tttcaactgc acccttgaaa tagctttcaa ggtgtgctat atttttatcg
    10321 aactattaaa acgttttact aaaacgtttt aaaaaataat tttcccatca aaataaatga
    10381 gatagttgga ggtttgtaaa atgaacacgg ttacagtaat tggaagtatt aatatggaca
    10441 ctacccttcg tttaaccaac atgcccaagc caggcgaaac gatgcatgcg catgagattt
    10501 ttcacgctgg cggaggtaaa ggtgccaacc aagcagtggc agccaaacgt tcaggcgcac
    10561 ggacatcttt tattggaggc gttggggctg acagcgaagg acaacagtta ttagatctac
    10621 tgacaaaaga aaacatcgat acatctggga tagctgaaat ccaaggcgcc acaaccggac
    10681 aagcaatgat tatggtagac gcagcaggag aaaatagtat cttgatccat gcaggggcaa
    10741 ataatgcttt ccatgaacaa gaagtcctaa aaaacaagca actgattaca aacagtgact
    10801 ttattattgc tcagtttgaa agtagcttag atgcgacgat cctagctttt tctattgcaa
    10861 aagacgcagg aaaaacaacg atcctgaatc ctgcaccagc tcgtgaaacg attcctactg
    10921 aactacttga aaagacagat atcattatac caaatgaaac agaaacagaa atcattacag
    10981 gaatcagagt gacagatcat aacagtttgg tcgcagcagc cgaaaaactc catgaattag
    11041 gtattggaac agtaatcatt acactaggca gtgccggcgc tttctatcat acagaaaaag
    11101 aacacggtat cgtccctgct tttaaagtgg atgccgttga cacaacagcc gcaggtgata
    11161 catttattgg cgcattaagc agcacacttc agccagacct tagtaacttg aaagaagcga
    11221 tcctttatgg aaatctagcc tcttctgttg ctgtgcagtc ttatggtgcg caaccttcta
    11281 tcccttaccg cgaagcttta gaacaatcag cagtctaaaa ttaacaatga gaggaagaaa
    11341 caaacatgaa aaaaggaaaa gtaatcaatt cagatatttc acgagtaatc gcacaaatgg
    11401 ggcactttga taaattaagt atcggggatg caggcatgcc tgtccccttt ggtactgaaa
    11461 aaatagattt ggctgtctca aacggtcttc caagtttttt atctgtttta gaaaacgttt
    11521 tagaagaact tgaggtacaa tcgattgaat tggcggaaga aataaagaca atgaacccgg
    11581 aaatattgga aaaaatcaaa ttattgttac cagatacacc gattagcttt atcccacatg
    11641 aagaaatgaa aaaagagttg aataactgtc atgcatttat tcgtacaggc gaaatgacac
    11701 cttacgccaa tattatatta gtgagtggag tggtcttcta atggaaacga caatgaatcc
    11761 tgaacagatg gtactgtccg agcagattca tatgctgatt gaacagttgg gaggaaaaga
    11821 caatattgat tctgtccttc catgtgcgac tcggcttcgt atcaaattga aaaaacagcc
    11881 agtagccaat gcagaaaaat taaaacagca aatcccttcc attaagtatt tcattttact
    11941 cgatagatat gcagaaataa tcatggatca gggattggct cctgcttaca aggaattgac
    12001 gcgtcaaatc gatcattcag ctccgaaaga aaagctaaat ccgatcgaaa aactatttgg
    12061 tttgatatcg gaaatcttta cgccgatcat tccgctgttt gccggttctg gtatattaaa
    12121 aggtctggtt gttttagcga ctacgattgg ctggcttagt gaaacaagcg gaacttatca
    12181 tattttgtct gctgcgtcga atgccgtctt ttattttctg ccaatctttc ttgctttttc
    12241 tgctgccaaa acgtttaaag tgaatggata tattgcggct gtcattgctg cagcactgat
    12301 tgaaccgaat ctgacctctt tactttcaga cagtggcaaa gcagctactg attttctggg
    12361 gattcctgta gtactgatgc agtatacgtc tactgtcatg ccggcgattt taggtatttt
    12421 cttttattct tttgttgaaa aattcctcaa aaaatatatt cgcgaaaata tgcaattagt
    12481 atttgtacct ttactttcat tagttatcac ggttcctttg acgttaatgg tattcgggcc
    12541 tgcgggtgtt taccttggcg aaggtttagc tgccgcaatt acttggatga tggatattaa
    12601 cggaccgatc agtggcgcaa tcatcgctgg tggttggaat attttggtaa tatttggtat
    12661 ccaatgggct gtaaaccctg tcatgatcag taatatctct gcttatgggt tcgaccgaat
    12721 cgttccgttg acaggagcag ccaattttgg aatggctggt gcagctttag gtgtatttct
    12781 gcgaagtaaa cgttcaaaaa cccgttcgat ctccggttct gcttttgctt cgattttact
    12841 tgcgggagtg accgaaccaa ctgtttacgg aattgcgatc ccattgaaaa aaccatttgt
    12901 tgctgcttgc attggtgcag ctgctggtgg agctgtaatg ggatttgccc aagtgaaagc
    12961 gatcgctttt gtatttggta gtctgaccac attgcctgca ttcatttccg gtactttttt
    13021 ctggtactta gcaggactgg ctgttagtct agtcgtagca atgatcacta ctctcgttag
    13081 cgggtttgat gaagacctaa tgtcttatga atagttaatg aaaaagactg aagtatgaga
    13141 tgtttatcac atatttcagt ctttttcttt ttaggatcat tatttttata aatttttgtg
    13201 atactttcca aactttggta taatttacat atgcataacc atttttatcc ccacttgttt
    13261 atccccaaac aaaggtggtc tatgcaataa aatgaacgtc aaagaaaaac gaatgaatgc
    13321 tcgttgctta tttacaaatt gttttattta gaatacacag acagatttat gaaaagaaaa
    13381 agcttttcga cacgattatc tgttccaaaa aatgtctaat gaatgctgaa taagacagaa
    13441 aatcaatgaa aggaaaagga ctagtttatt atgaaaaata aaaaaacgat acgtttgcta
    13501 gctgctactt ggctgattgc tccgtctgtt ttgtcgacca ataccgtatt tgccgtagat
    13561 aatcaaatag ctgatcaaac agtcacaaca gatacgacta catccagctc agctactagt
    13621 gaaacaacgg attcttttac agaagaccca actggaacaa ctaactcaac aacaggatcg
    13681 accgagccaa ctgaaattga tccggctgat ccatcagaaa atgaaaagcc agccgagaca
    13741 tattccctta caattgctcc ttctttgatt ggtaagatcc aactgactgc ttcgacagcg
    13801 gattcccctg aacagcaacc tttggatatt acggaaagtg gaacggtaga aggtttatta
    13861 gcggaaacga aggtaactta cactattacg ccagctgaag gtgaacaaat tgcttcagtc
    13921 gctgttaatg gtgccaaaga agtaaattat gatgcaaaga cctttacagt aggtacgaac
    13981 aatttatcta ttactgcaga atttaaagct ggaccggata cttcaacaga gaaaccaact
    14041 gatcctagtg aagaagtccc tagtacacca gatccggaaa cgccaaacaa accagatgtg
    14101 ggtaataaca acaataataa tggttcaaat aattcaaaca ataataacaa taattcgaac
    14161 agcgggaatt cttcgaatca aacaaaccca tctggatcga ataacaatgg aaatactcag
    14221 agagaaccaa accatccaaa caatggttca tcgaatggcg gaacgaacaa tgcttcatct
    14281 cgtccaaata cagcaaatca aagcggtata gagaatccat cttctgcttc ttctgatttt
    14341 gttgtaaaat caccaattga tgctgtattg cctacaaata caacgaatgt ccaacaagca
    14401 atcgttcgag aagcattcaa gcatcttgga aaaccatatg tatggggagc aaaaggtcca
    14461 aatacttttg actgttctgg tttgacttat tacgtatata tgaaagcaac cggtcattat
    14521 atcggtggct ggacaggcga acagcaatat gcaggtacgc agattccagt aagtcaggcc
    14581 caacctggcg acctcgtctt ctggggacct tcttccggtg ttactcacca tgtaggtatc
    14641 tatattggga acggacaatt tatccatgcg cctcaaccag gagataaagt tcgagtaact
    14701 tctatttcag atttcacgcc tgattttgct gtccgagtaa acttagcagg tttacctgct
    14761 gcttcaggtt cgctgacagg cggctcatct attttagatg gattagatgg ctcgttccat
    14821 ttcagtcaaa accaaacaac tgatcagttc ttgaaaaaga ttgcagatga tgcacaggaa
    14881 atcgggcaaa aagaaggaat ctacgcatca gtcatgatgg ctcaagctat ccttgaaagt
    14941 ggatctggaa acagcttatt atcaagcgaa ccaaatcata acctttttgg tatcaaagga
    15001 tcatataaag gaagcagcgt gaccttcaat acacttgaac aagatagttc cggacaaagc
    15061 taccaaatcc gcgcacaatt ccgtaaatat ccttcttaca aggaaagttt ggaagactat
    15121 gccgacttga tcaaaaatgg actgactggc aatcctgatt tctacaaacc aacttggaag
    15181 tcggaaacaa aagactacaa agaagcaacc aaatatttgg aaggacgtta tgctacagat
    15241 cgccaatatt cacaaaaatt gaacgcgatc atcgaagcgt acgacctgac aaaatatgat
    15301 gaaccgaaaa aagaagcagc aacaactgaa gaagcaaacg ctgaaatcct caatatgtct
    15361 aagcgattcg atgtgccaat caaatggaca acagatggcg tgccaatcac aggattagaa
    15421 ttttcaacga taaaacgcgt caacaacgct tcttccatct tccgtgtcgc ttcaatctgg
    15481 gatttgtgga acaattttac ggcaagacag ataccagaaa caactactcg tacagttaaa
    15541 gctgcaactg ttcctgtact aaccttgtta aggttagaca aatcattctg gcttgatctg
    15601 cctttaaaga aatagaataa attaaaaagg ggatgtgaca ctgtcacact ccctttttaa
    15661 tttattctat ttctttatat ttattttgta ttccgttaca aactcgcgta cttggttagg
    15721 ttccagagtg acatctccaa aactttcaag tcgctccgct cctggcagtt cttgagtctc
    15781 aaatgtgata ccgccatgat ttactaattt attcccgcgc atttccggtc catcttctcc
    15841 aaaatttgct gtaaaaatca cgattcctga agcatctgtt tttacttgga tcgatacttt
    15901 ttcatctggg cttgtcaatt ccgcagcatt tagtaaacca gattgttttt tcaacaaaaa
    15961 tggatgatcg atgccgccca ctatcattga ttggcgaaaa tcactggcaa agacctcacc
    16021 taatttttta ggacgttgga agtcaaatgc tgtgccactt actgaaacta cttctcctgt
    16081 aggcgttgta tctttattga gcgtagcaaa taatcggcta ttcaaccaaa gtatatgttc
    16141 gtcgattgac tgtgctggat caccggttaa attgaaatag acatgatttg taggattgaa
    16201 tactgttttg cggtcactga tcccttggac cgtcactcgc catgtatcat cgttcgtcag
    16261 cgtataccgt acttctacat ctaaagttcc aggaaatccg ttttccatat ctgggctagt
    16321 ctgatagaaa atgaccgatg cttcgttttc accattgatg actgtataat gccactgctt
    16381 ggcttcaaag ctaggtgcac caccatgtaa acaatgacca gtgtccgggt ctgtttttac
    16441 ttcgaacgtt tcatcatcta atttgaatgt gccattcgta atccgtccag ccatacgtcc
    16501 gattgaagca ccgatataag ggtctttttc tagatattct tttgcttcat caaaacctaa
    16561 gatcaactct cgtttttcat tatcgattat tgtttgaaaa tgaacaaccc gtgccccaag
    16621 atcactgaca gacaaagatg tcccattttg attcgtgatc gtaatcaagt gatagttttc
    16681 gccaaaattt tgttcattga tttccatgtt ccttgcaccc tcttttcttt catttgcact
    16741 gattccagta tacatggaaa ccgttttctt aacagcttat tccgtgagta acgagaaaaa
    16801 atttactata ttctaataaa agaccgtgaa aaaaaggttg tgacaaaaat attgtcacaa
    16861 cctctttttc cgaacaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa
    16921 acgaaaaata tgaggagcta tttttgcttg gctgttctta ttacttgatg caggacagct
    16981 ttttcacaat ctctttttca atacgattcc agtatataac catgtcgtca gtacatagta
    17041 gatgaaatac aatacgaaga atatcgtaaa cggaacccaa atatctttgt atggattttg
    17101 catgaataga ccaaacattt gtaatccgaa taagacatgg actacaccta agattcctgg
    17161 tgctaagaat aacacgccta cttctctacg gatcgattgt ttaagcaacc cgcgtcgtgt
    17221 tcctatcttt tcaagcattt cataacgagc aatgtcactc ttgcttcctg acaagatctt
    17281 gaacatcaaa cagctagcaa gcatggtcaa gaaagctaaa cctaggaaga atcccataaa
    17341 ttcaaaacca gagaatagac cattatacac ttcatacatt tcatatttct ggttgctgta
    17401 ctctactttt tgcaaagatg gattgttcat tttattttga gcaacaagtg tttcgattgg
    17461 tttcaggttt gcttggaagt cttttacctg aactagctgt aaagtggatt gtggcaagtt
    17521 cagttgatca aattctgatt gactgactac cttgatttct ttttcttgct gttccggcaa
    17581 ttcatagctt cgaagttcct cttgtacagt cagttcattc atttttttag caggaacttt
    17641 ttctttcgtg atcttcggcg gtgttccatc ttcatgtttt atcatgatca atggttgagc
    17701 cgcaaattca cttgcattat agtaaatcgt tcccgcatct tctttttgag tataagaagc
    17761 agaaagtgtt ggtgaaagtt ctttgatttt ttgctgatct tctgcccgag gattattcat
    17821 cactagatca tatccagaat tgttttctgt caagtctgca atttcatttt taaaacctaa
    17881 tcctaccgtc aaagcgccca gtgccaaggc aaacagcata gcgaccattg agagcatttg
    17941 tgtatattca cgaatacgaa agtttaactg tgacaatgtg aaattgttca attttttcaa
    18001 tgaaatctgt tctgatttct ttagtaatga taaaaagaaa atgatcacag aatggaaaat
    18061 gaaataagtt cctaatacga tcgtaactaa ggctacccct ataccaaaga cttgataggt
    18121 ccccacgtgt ttcatcatat agtaaccgca gcctagaaaa gcaattccca gtactacttc
    18181 aaggaaaaac aagaatttat gctgcttgat tcttgttggt gtctgatccg cacgaatcaa
    18241 tgctaaaatc ggttttttga caatcgaaaa agcattgaca acagcagcta ataaaaacaa
    18301 aacagcaaaa aacaaaacag tgatcaacaa cccgtgaata ctaaaaggtg taaaatgcga
    18361 aatattgaga tctaactgat ccactaataa tcggtggacg atacttgtca atcctacacc
    18421 gacagctgaa cccactaaag tagctgcgat ccctactaag aatgtttcag aaaaaatcat
    18481 ctgtgcgatt tttctgcctt ttgctcccag catcataaac attgcatagt ctttttgccg
    18541 catcgtcatc aaaaaggaat ttgcatataa aatataaacg aatgtgatga tccctagtaa
    18601 aacagaaccg aatcggaaaa tgataactgt agccgcaacg atcgtattac ttttcaaaaa
    18661 agcttcgttg cttgccattg attcaaacat gtaaaaaata gcagatgcca taactaatcc
    18721 tgagaataac accaagtagt ctcttaaacg gctcttgatc ccagtaagtg ataatttcca
    18781 aagcatagtc aatctccccc tactgttcca agtttccaag aatagatagt atttctcgat
    18841 agaaggaatc acggctttga tctccacgtt tcacttcttg atggataccg ccatccttga
    18901 tgaataaaat acgttggcaa taacttgcag aaaacggatc atgcgtcaca agaagaatcg
    18961 atacatgatc ttttgtattc aattcgtcca tcgtatctaa aagatcccgg gcacttttgg
    19021 aatccagcgc acctgtaggc tcatccccta atagaatcgt tggttgtgtg atcaatgcac
    19081 gtgcggcggc gacccgttgt ttttgtccac cggaaatctc agaaggatag ctttccaaaa
    19141 tatgagagat ggataaacgt tctgcaattt tttggacacg ttgcttgatt tcttttggtc
    19201 tgacgccttg taaagaaaga ggaacagcga tattttcttg tgctgtcaag ctttctaata
    19261 aattgaaatc ttggaaaata aagccaatct cattcgcacg aaaatctgct agttgatttc
    19321 cttttaatgt ggtcacatct ttttgattga ttcggatatg cccatctgtt ggtttatcta
    19381 atgtggataa aatattcaaa agtgtcgact taccagaacc agaagcgccc atgatcccaa
    19441 taaattcgcc tttttctaca tcgaagctga tgccgcttaa tgcttgcgtc tttttctcat
    19501 tgttttttcc gtatactttt gttacattct gtacttcgac tattttgttc atttctttcc
    19561 ctacccttta attagttttt ccagtgctgt tttcggcacg tctttttttt cgacttcttc
    19621 atacgaagtg acacctttat ttttgttcca ggtgattttt aaatatgctc ctttacgaag
    19681 cggtcgagtc aattgtccct tgaagtccag ttctttttcc ttaccctctt tcgtatagcc
    19741 aggcaggata tatctatagt tgactgtttc ttgtccagag tctgattttg aaactgtttt
    19801 tgtaccatct gtcgtaattt gtacatagta ttcttctcca cccataacta cgtaatctgc
    19861 gattttcacc cctgcaagta ccacaacgat gaaaacaagt agaccgatca gaagtttttt
    19921 cactttttcc ccttctctct tactttatat actgattata gaaaaaagcg aacaattctg
    19981 ccttcgaatc aactatcatt cttctttcaa ttttgtcatt ttctttaaaa caactaaaaa
    20041 aagagttcaa gacaataatc cgtcctaaac tccttcttcc tatcttacaa aaagtatgtc
    20101 acttacggtc tttcatgtcg cctgtattga taacaggttg cgttcctttg tctgtaatga
    20161 ttgagacaag taaattattg atcaattgga ctttccgatc atcggtgaaa ttgatttctt
    20221 gtccatcttc gatttgctct aaggccattt gagtgattgt gacagctcct tcaacaatgg
    20281 tttgtcgagc agacaaaatg gcttttgctt gttgacgttg gagcatggca ctagcgattt
    20341 ctgttgcata ggctaaatgg ttcaagcgtg tttcgattac ttcgacacct gctactgcta
    20401 aacgctcttg cagctctttt tgcaactcgt cagacactgc cgtcgtgttt cctcgaagcg
    20461 tcaaatcatt ctcattgaat gtatcatacg ggtattggct agcgatatgc cgaatggctg
    20521 tctcactttg gatttctaca aaatcttgat aatacgctac atcgaataat gcctttgctg
    20581 tatcaacgac tttaaagact actactgctg agatttcgat ggggtttcca tccaaatcat
    20641 tgacttttaa aacagaacta ttgaaattcc gaactttcaa cgaaacagtc attttttgtg
    20701 ccaaaggtat cgtaaggaaa aagccatttt ctcgaatcgt acctaaatat tgtccaaaaa
    20761 acaaaattac ttttgcttga ttaggactga ctacagttgc cgaacttaac aacaataacg
    20821 tgatcaacca aagaaaaata cttaaaaaaa gtgcccaaac tttcgcttgc cacataccta
    20881 aatagaagaa aaacaatcca actaaagcta atacgaccaa tccgacaatc cctgcataac
    20941 cattgatata aaatgctctt ttttctctca tgctacttcc tgcgctcctt tatccttcaa
    21001 taaatatcta atgttacttt tttaattttt ttttcgaata cgcgccatta caccttctag
    21061 ttctaactct ttgtcttcag tgatgtacca accgcttgcc tgtgtccctc cggctgcctg
    21121 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21181 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21241 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21301 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21361 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21421 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21481 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21541 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21601 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21661 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21721 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21781 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21841 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21901 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    21961 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22021 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22081 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22141 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22201 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22261 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22321 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22381 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22441 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22501 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22561 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22621 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22681 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22741 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22801 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22861 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22921 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    22981 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23041 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23101 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23161 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23221 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23281 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23341 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23401 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23461 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23521 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23581 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23641 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23701 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23761 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23821 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23881 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    23941 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24001 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24061 atcccattac ctaaatgaac aatcaaacca atcgctgttc ctatcactaa agcaaaaata
    24121 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24181 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24241 ccaattagtc cgccaaacaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24301 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24361 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24421 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24481 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24541 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24601 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24661 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24721 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24781 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24841 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaag
    24901 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    24961 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25021 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25081 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25141 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25201 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25261 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25321 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25381 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25441 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25501 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25561 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25621 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25681 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25741 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagta caatctctgt
    25801 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25861 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25921 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgaa aaaaaacgat
    25981 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26041 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26101 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26161 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26221 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26281 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26341 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26401 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26461 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26521 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26581 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26641 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26701 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26761 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26821 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26881 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    26941 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27001 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27061 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27121 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27181 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27241 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27301 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27361 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27421 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27481 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27541 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27601 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27661 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27721 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27781 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacaaatcat atgcataaaa
    27841 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27901 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    27961 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28021 ataatcctga actggcccat caatacgcag atcgaatcat tgaattttct gatggtaaga
    28081 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28141 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28201 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28261 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28321 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28381 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28441 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28501 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28561 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28621 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28681 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28741 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28801 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28861 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28921 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    28981 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29041 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29101 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29161 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29221 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29281 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29341 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29401 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29461 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29521 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29581 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29641 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29701 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29761 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29821 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29881 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    29941 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30001 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30061 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30121 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactcctcc
    30181 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30241 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30301 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30361 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30421 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30481 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30541 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30601 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30661 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30721 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30781 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30841 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30901 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    30961 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31021 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31081 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31141 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31201 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31261 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31321 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31381 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31441 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31501 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31561 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31621 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31681 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31741 taagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31801 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31861 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31921 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    31981 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32041 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32101 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32161 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32221 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32281 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32341 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32401 gttaatcacc atacaatgaa acttttggat caatttgaaa ttgtaaatga gcattcaaaa
    32461 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32521 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32581 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32641 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32701 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32761 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32821 gattttagat tggttggaaa aaatagattt ccatatcaaa taaccagttt attataaaga
    32881 tggtgaaaaa ccattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    32941 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33001 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33061 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33121 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33181 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33241 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33301 ttcacgatac tgccttcata cggcacattg tcaaaagttg cttggacttt aactcttcca
    33361 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgccgact
    33421 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33481 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33541 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33601 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33661 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33721 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33781 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33841 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33901 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    33961 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34021 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34081 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34141 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34201 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34261 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34321 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34381 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34441 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34501 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34561 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34621 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34681 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34741 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34801 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34861 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34921 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    34981 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35041 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35101 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35161 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35221 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35281 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35341 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35401 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35461 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35521 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35581 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacac
    35641 cgcaagcttg gatcaaacga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35701 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35761 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35821 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35881 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    35941 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36001 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36061 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36121 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36181 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36241 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36301 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36361 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36421 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36481 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36541 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36601 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36661 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36721 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36781 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36841 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36901 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    36961 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37021 ttaaccggta gttcattggt gggtgtatga atccactctg ccaattgttc cttcccttct
    37081 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37141 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37201 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37261 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37321 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37381 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37441 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37501 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37561 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37621 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37681 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37741 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37801 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37861 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37921 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    37981 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38041 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38101 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38161 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38221 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38281 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38341 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38401 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38461 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38521 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38581 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38641 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38701 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38761 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38821 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38881 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    38941 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39001 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39061 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39121 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39181 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39241 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39301 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39361 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39421 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39481 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39541 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39601 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39661 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39721 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39781 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39841 gtttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39901 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    39961 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40021 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40081 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40141 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40201 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40261 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aatagcttct catattttct
    40321 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40381 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40441 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40501 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40561 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40621 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40681 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40741 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40801 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40861 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40921 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    40981 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41041 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41101 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41161 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41221 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41281 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41341 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41401 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41461 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41521 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41581 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41641 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41701 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41761 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41821 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41881 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    41941 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42001 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42061 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagcaaa
    42121 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42181 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42241 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42301 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42361 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42421 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42481 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42541 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42601 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42661 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42721 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42781 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgaaattgg gagacggtaa
    42841 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42901 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    42961 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt accggacaga
    43021 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43081 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43141 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43201 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43261 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43321 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43381 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43441 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43501 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43561 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43621 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43681 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43741 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43801 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43861 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43921 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    43981 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44041 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44101 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44161 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44221 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44281 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44341 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44401 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44461 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44521 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44581 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44641 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44701 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44761 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44821 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44881 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    44941 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45001 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45061 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45121 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45181 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45241 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45301 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45361 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45421 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45481 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45541 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45601 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45661 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45721 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45781 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45841 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45901 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    45961 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46021 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46081 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46141 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46201 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46261 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46321 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46381 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46441 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46501 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46561 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46621 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46681 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46741 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46801 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46861 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46921 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    46981 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47041 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47101 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47161 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47221 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47281 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47341 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47401 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47461 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47521 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47581 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47641 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47701 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47761 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47821 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47881 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    47941 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48001 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48061 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48121 gttctcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48181 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48241 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48301 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48361 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48421 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48481 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48541 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48601 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48661 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48721 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48781 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48841 ttgaaagaaa agtggcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48901 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    48961 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49021 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49081 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49141 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49201 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49261 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49321 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49381 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49441 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49501 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49561 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49621 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49681 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49741 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49801 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49861 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49921 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    49981 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50041 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50101 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50161 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50221 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50281 ttcagatagt caagatactg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50341 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50401 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50461 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50521 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50581 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50641 cttcgccctt cgcacgatgc agttggcgca aatgtatggc tatgtcattc cagagacact
    50701 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50761 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50821 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50881 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    50941 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51001 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51061 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51121 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51181 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51241 aagacaggtg caagaatcga gaaaaaggcg aatggcaagt aatgcaacgc gctaactttc
    51301 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51361 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51421 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51481 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51541 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51601 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51661 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51721 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51781 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51841 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51901 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    51961 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52021 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52081 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52141 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52201 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52261 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52321 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52381 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52441 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52501 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52561 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52621 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tattttttta tttttcttct
    52681 aaactttttt ctacctcaat cccccaagaa tctttcttga agtgattgat gatcaatgga
    52741 ataacgaaca tgaccactgt gatagctgct actaataatg catcactcaa tgtcgctgta
    52801 cctaatgtag caaatacgac aacgatgata gagaagatca gcatgcagct gactacccaa
    52861 gctaatccgt ttccgttttt accaattcgg taaggacgtt cgaattctgg atgtttctta
    52921 cgcattttaa gcaatgaaac agcaatcaaa atgtacacga tacaataaat aacagttgta
    52981 gcatttgtaa gtgtcaagaa tacagcactt acgtcatcca ttacaccata aagcaaagca
    53041 aataatgaaa taacgattga ttgtgttaga acgacatttc tagatacgcc gtatttgttt
    53101 tctttatgga atccgaattt tggtggaagg aatccttcac gagctacttg gatgattgtt
    53161 tttgatggtc cagtaaccca agctgataat tgtaaaagca caccaataaa taccatgaag
    53221 ctgaagatat ttccgatcac ttctggtaag cctaggattt gacagtcaat caagataggc
    53281 tgcgtgatat ttgctaattc catttttcca tctggtacga catttgctac aagcatcgca
    53341 ttgatgacat tcaataaaac taatccgatc aaagcgataa atacgccttt tgtataattt
    53401 tttgttgcgt ctttcaaacg aggcatgtaa acagaactca tttcgatccc aacaaaaata
    53461 aatgtgatac cagctaagta tttcaatgaa tcaatatgtt ccaagtcagg gaaagctttt
    53521 gaccatgaaa agtcacctag atagccgttt gagactagtc cgactttgaa ggctgccaaa
    53581 acacctaaaa cgaacatgat gactactggg atatataccc ctagccatac accgatgttc
    53641 ccgccgactt ttgccatatc gaatttcaag ttcaagatcg tgatgatcca gtagataact
    53701 aaaatacaac ctaaaacgaa ccagtgatta ttgccaagtt ctacattacc aaatgtgttt
    53761 cctaacaatg gtcctaaagt agaagcgacc attaccatac ctgggaacat ttgcacccat
    53821 aatagccatg caacgacaaa tccccatttg ctgcccagtg ctgtttttac ccataactga
    53881 ggaccgcctt cttgaggaag catcgtggaa agctcaccgg aaaccattga tatcggaccg
    53941 gcaaaaaaca aaactgcaaa aattgaataa aaaatcaatg tccaaccgac tgcggctaac
    54001 gtaggaatac tccgaaccgt tgcacacagt gccattgtca gaccgataaa ggtcccaaga
    54061 cccattttac ttacttcaga tttactcacc gttgacactc tccttgattt tttggataaa
    54121 ataacggtgt ctttgaagta gtttcagcaa taaacgaaac aattcagcga atatttgagg
    54181 ggattctttg agtttattgc ttgaagggtt tcttcaaaga caacctcaaa acaaagggat
    54241 cgaagagctt aggtctcccc ttgctcttca gatccaaaca tgcgtcagaa acagttagta
    54301 ttcaagctgt tttctaacat gattaccttg cttattttgc ttcgcttgcc aataattggt
    54361 ctgcgtagat tttttccaat ttttcttgaa gagctgcttt gattttttca gcatattctt
    54421 caaagtgttc ttctttgttc atgtatggtg tcattacgct ggcacgcaat acagttactt
    54481 ttccggcacg gttccattct tcatctgaga atcctagttg gttcacaaat tgtaatgggc
    54541 tgttgccata gtctgggata gcaaagtcag tatgtgaagt taagaactca ttaccgtaaa
    54601 tgcttccttt cacgtatgaa gaataatcat atacgtcatg gttcaattta ttcattgcaa
    54661 ctaagtcatc gttgcctttt tctttgaata catagtctac catattgaaa tctggataag
    54721 ttaatggatg aacttcgatt tctttgtctc ctactttaaa tgacaagtca ttcaagaagt
    54781 tgtagaaacg atgtgcacct tcgattgatg cacccattaa tttaccatat cctgtgacat
    54841 tcaaaggtaa gacatggtgt gctgcccaaa cgctggctgc tgttgcccct gcttttgaac
    54901 cttccaagat ataagcccca agtaatgctg ggatatctgc gcctttttca aatacatatg
    54961 tcgcaaagta agagataacg tctctcatgc ggatatcttt gatgacgata ccaccggcag
    55021 aatatggaac atagcccatt ttatgtgggt caatcgttac agattctgct tcttcgattg
    55081 ctttatatgc actgtgtact tcttctaaga tataatcttt gttttctgta aagacattgt
    55141 atttgtaatg aacatctttt aaatcttcaa atggaatgaa gttgttgtct tcgtctaaga
    55201 aaatcgcacg gccgtatccg ccataagcag catctacgtg taagtagaag taaataccgt
    55261 ctttttctaa tacgcgacgc aatgcaacga ttttatcgat accgtcgatt gctccttctt
    55321 ctgttgatcc tacaacaccg actacaccta ggattggtgt tttttcagct gctagaccac
    55381 gaacgatttt ttcaagttcg ttgatgtcca tgcgatagtt gtggtcaact ggcactggaa
    55441 tcacttggtc taaaccgata ccgatgatgt cggcagcttt tagccatgaa tagtgtttag
    55501 tttgtggaac caaccatttt cctaattttt ctaaatgttt tccgctacgt gctgagtgag
    55561 ctttgatttc gtcgattttt tctgggacgc tgtctaacag attcatgatt tcttctgttg
    55621 acaagttcat taattcccaa tcgctttttc cagcaactag ttcaggtgtc acttctttca
    55681 ttgcaagcgg caatgatttg atgttgcggg cataccaaag tccttctaag tttgctaaag
    55741 aaccatcagc aacgatgtgg ccccagccat ctttatagct cattaatttt gcaaattcca
    55801 ttcctacttc ttcttccatt tggcttgttg caggagaaga ttcataggca acgttattac
    55861 cattccatag catagcaaaa ttgtacgcta gtaaagatgg catcaatgtt tcagagttca
    55921 tatgacccca gtaacgtccg gcattatgcc aaggaacgga atgggtacgc atccgcgctg
    55981 aaatttctga tagaacatcc tttgttttat tcacagtgtg ttcaaaacta gcactgcttt
    56041 tttcttctgg cgtaataata ggcatgtctt gaggcatgta gttttgacgc cagcctaaat
    56101 gttcatccac caattcattc aacaaagctt tatagatttg gccgttctca gctttgtccc
    56161 caatgaatag ggcatttagg tttagatctt tcgacaatga ttcactcata gtgaaaccct
    56221 cctaaaaaaa tatttaaatt ttgtcgagcc ctacattaca cctgttcttc tattctgtaa
    56281 actaagtttt ggcctgattt ttaatcatat tttaatatgt atcaattatc cgaattgttc
    56341 acacttttga aataattaaa aaaaacgagg gaatttacaa atgtaaactc cctcgcaaag
    56401 attgtattaa cgacaaccag cgttctattt taggatgggt ttcaaaattg gaatgttgta
    56461 atagtatttc cctttttcaa ggtaaaaact tactaaattc taatcaatta ctttggccaa
    56521 aaagtaattt ttcttacctt ttcgaataac cacaaactta ccatcgaatt catcggatgg
    56581 atttatgaca aaatttaaat cggtaacgcg gtcaccgtta atacttatag ctccgtttga
    56641 aacatcttca cgtgcttgac gtttagatgg ttcaattttt gtggaaacaa gcagttccac
    56701 gatattttca ggtgtgcttg aaatttcaac gttcggcatt ttaccgaatc cttgcgcgat
    56761 ttcttccgca tttagttctt taatatttcc agagaacaat gcttgcgtta ttttttgcgc
    56821 ttctttcaag tcttcttcac tatgcacaaa tcttgtaact tcttctgcca aacggcgttg
    56881 tgcttcacgt ttctcaggtt ctgtctctac ttttttcgct aagtcttcga tttcttcttg
    56941 agacaagaaa gtaaagaatt tcaagtattt cactacatcg cgatcatctt gattcaacca
    57001 gaattggtag aactcaaatg gtgaagtttt cttcggatca agccaaacgg caccgcctgc
    57061 agtttttccg aatttcgttc cgtcagcctt cagcatcaat gggatcgtta aaccgaacgc
    57121 ttctgcttct ggtccttcta attttcggat taggtctaat ccagctgtga tatttcccca
    57181 ttggtctgct ccaccgattt gcaattgcac atcgtatgtt ttatgcagat gcaaaaagtc
    57241 gatagattgc aggatttggt aagtaaactc tgtaaatgag attcctactt ccaaacggct
    57301 ggcaacgata tcttttgcta acatcgtgtt gatgttgaag tttttaccgt agtctctcaa
    57361 aaattcaagt aaagagattt ttgacaacca gtcgtagttg ttcacaaatg tgatatttgc
    57421 atctttacca aacaattttc tcatttgatt ggaaagtgcg tccacgttat gttgcacttg
    57481 ttccatcgtt tgcaacacac gttctgtttt gcggccgctt gggtcgccga tcgttcctgt
    57541 tccgccgcca atcagaatat atggatgatg gcctgccaat tggaatcttt tcatcatcat
    57601 aaatggaatc aaatgtccga tatgcatcga atcaccagtt ggatcaacgc cgcaataaag
    57661 tgaaatgctc ttttcttcaa cgagctcgcg aagtctttct tcgtttgttt gctgattaat
    57721 ggcgtcgcgc caagttaatt catcaataat attcatttta ctgacctccg tgtagttttt
    57781 ttatgaaagc aggaacctgc tagcatatgt aattaattat ttcttcagga taggatagga
    57841 aataaaaaaa gtcccttggc atataagcca agggacgaaa tctcgcggta ccacccaatt
    57901 tgtatgtcta gatatccaac atacctctcg aacatttaac gctgtcgcgt ggcaatcact
    57961 gccaaagact cctgaaatgt aattcgcttt aaagcatgta ctggtttgca gcgaccacca
    58021 gctttctgaa acattggctt taacaactac ttcgtttcga tcttcgtctt gttattatta
    58081 atatgtgtct atcatagagc gcaacttttt ctttgtcaac agaaaaagga tatttttttt
    58141 aaattcctca ttttttcttt tttgaatccg ctctaattcc gaacatttta ttttcaatta
    58201 ttgttaaaag aaataacttt ttctgtattt tttctgcaat ttatcgagaa gtcactttac
    58261 tttgcctctt attctttctt cgttacaata aggaatggga caagtaaaaa aatgaaaaag
    58321 caagggacga ggtatatgga aaaaaagcaa aaaattattt taggagccac ttctgcttta
    58381 actatcgtta ttttaattct aagtttgttt tttgtcatcc gatcatctac aaaaaaagaa
    58441 ccaatcattc ctgaaaaaaa aataacagaa aaagcaaaag aaccgacaga tgataagcaa
    58501 caactagtta ctcaagcaaa aaatctggct caaagctacg attatgacca agcgattgcg
    58561 ttattggaaa aagatagtaa gaacggatct actgaaacac aacagcttct tcaaacacta
    58621 aaaaaggaga aagaacaact gatcgactgg tctgatccta ctcagatctc tcatgtcttt
    58681 ttccacagct tgatcgtgga cccgcaaaaa gccttccact ctcaacaggc acaaggatac
    58741 aaagattata tggtgactgt tgaagaattc aaccgttcga tcgaacaact ttatcaaaat
    58801 gattttgttt tagttaattt gaatgatttg atccaaaaag acgaacaagg aaatctttcc
    58861 tttaacgggc ttgcgctacc tgaaggaaaa aaaccattgg ttctttcaca agatgatgtc
    58921 agctattatg aatatatgga taattccggt tttcctagcc gattggtcgt atcaaataac
    58981 aatgaaatca aaaatgtcta taaagagaaa gataaagaag aaatcggtga ctttgacatg
    59041 gttcctctga tcgatgcctt tgtcaagaaa catcctgatt tttcttatcg aggcgcaaaa
    59101 ggtaccttgg ccctgacagg ttacaacgga gttttaggtt atcgaacatc aaaatctcaa
    59161 tatggagaca atgaaaaaac aaaaaaagag attaaagaag ctaaaaaagt ggcggaacag
    59221 ttgaagaaag acggctggag ctttgcttcc catacatggg gtcacttgaa tatgactcaa
    59281 gcttcactag aagatgtaaa aaaagacaat gaattgtggc agcaagaagt tgcacctatc
    59341 ctaggaaaaa caaatattct gatttatcca tttggtgcag acatcagcga ctggcagcct
    59401 tattcagcag aaaatcaaaa atttgcttac ttgaagagtc aaggtttcga tattttctgt
    59461 aatgtcgatg cttctacacc agcttgggga caattaggta cggatttcta tcgaaatgct
    59521 cggatcaata tcgatggcat tcgttttcaa tctgatttag acggaaaaaa tcctattctt
    59581 gatccattca tcaatgtcca tgatgtctat gacaaagaag cacgcgaatc tgcttgattt
    59641 tacgtcattt atgaaccaaa aaagccgaac tgacatttta tcgtcagttt cggctttttt
    59701 atgctttatt tcttcttcgg tgcgattccc ttgatcagga cttctgtcca ttctgctgta
    59761 tatggaagca gttttgcgta tatctccggc ttagaaattc cttctaccat tgcttgaaaa
    59821 tagaggatca agaactcatc cgaatatttc aaatccactt ttccttcttt ccgccctcga
    59881 tggaaaagat caagcatgat actgaaactt tcttgcgtat acgcatccat cgcgtcagaa
    59941 aattcttttt tttctttttt tgtatagtat gtcatcaaat cgatataaaa ttgtttgttc
    60001 actcgattga gataacggat tttgttttca ctcatggcaa tcagtgtttc ttcaaatgga
    60061 agattttccg caatcaacgc tttcgcttct tgccctaaat cattcataaa tgcaaaaaat
    60121 acttcataaa tcagcccttc cttgctgcca aaatatttaa aaatcgtcgc tttacttacc
    60181 tgagcgttct cagctacctt atccattgtc actccagcgg ttcctatgtc tgtgttcatc
    60241 aagtcaaaag ctgcttgtaa aatctctttc tttttctctt gtgctctctt ttcaaaaccg
    60301 ttcatttgta gagcctcccg taattttaaa cttttataat atatatagtt caaatttatt
    60361 ctgaggttta ttataactat ctattgagaa agtatcaagc tagcaattcc ttgtttatga
    60421 attcattgac agaaatttat aaaaagaata ttatgtacta aaa
//
LOCUS       DAZAID010000005        56496 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000005 DAZAID010000000
VERSION     DAZAID010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.005"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACG9BE_000253"
     CDS             3..371
                     /locus_tag="ACG9BE_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFN3031042.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACG9BE_000254"
     CDS             551..1492
                     /locus_tag="ACG9BE_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFN3031043.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACG9BE_000255"
     CDS             1561..2292
                     /locus_tag="ACG9BE_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN3031044.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACG9BE_000256"
     CDS             2436..4616
                     /locus_tag="ACG9BE_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFN3031045.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACG9BE_000257"
     CDS             4636..5118
                     /locus_tag="ACG9BE_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFN3031046.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACG9BE_000258"
     CDS             5134..5895
                     /locus_tag="ACG9BE_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFN3031047.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACG9BE_000259"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACG9BE_000259"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFN3031048.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACG9BE_000260"
     CDS             7667..8152
                     /locus_tag="ACG9BE_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFN3031049.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACG9BE_000261"
     CDS             8145..8828
                     /locus_tag="ACG9BE_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFN3031050.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACG9BE_000262"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACG9BE_000262"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFN3031051.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACG9BE_000263"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACG9BE_000263"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFN3031052.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACG9BE_000264"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACG9BE_000264"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFN3031053.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACG9BE_000265"
     CDS             13125..13457
                     /locus_tag="ACG9BE_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFN3031054.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG9BE_000266"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG9BE_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFN3031055.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACG9BE_000267"
     CDS             14283..15656
                     /locus_tag="ACG9BE_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFN3031056.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG9BE_000268"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG9BE_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFN3031057.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACG9BE_000269"
     CDS             16711..17151
                     /locus_tag="ACG9BE_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFN3031058.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="ACG9BE_000270"
     CDS             17295..17996
                     /locus_tag="ACG9BE_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFN3031059.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="ACG9BE_000271"
     CDS             17993..19114
                     /locus_tag="ACG9BE_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFN3031060.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="ACG9BE_000272"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="ACG9BE_000272"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFN3031061.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="ACG9BE_000273"
     CDS             20493..21401
                     /locus_tag="ACG9BE_000273"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN3031062.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="ACG9BE_000274"
     CDS             21436..21708
                     /locus_tag="ACG9BE_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFN3031063.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="ACG9BE_000275"
     CDS             21719..22765
                     /locus_tag="ACG9BE_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFN3031064.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23016..25625
                     /gene="clpB"
                     /locus_tag="ACG9BE_000276"
     CDS             23016..25625
                     /gene="clpB"
                     /locus_tag="ACG9BE_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFN3031065.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25776..26462)
                     /locus_tag="ACG9BE_000277"
     CDS             complement(25776..26462)
                     /locus_tag="ACG9BE_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031066.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26440..26934)
                     /locus_tag="ACG9BE_000278"
     CDS             complement(26440..26934)
                     /locus_tag="ACG9BE_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFN3031067.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26954..28174)
                     /locus_tag="ACG9BE_000279"
     CDS             complement(26954..28174)
                     /locus_tag="ACG9BE_000279"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3031068.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28321..30156
                     /gene="lepA"
                     /locus_tag="ACG9BE_000280"
     CDS             28321..30156
                     /gene="lepA"
                     /locus_tag="ACG9BE_000280"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFN3031069.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            30352..31263
                     /locus_tag="ACG9BE_000281"
     CDS             30352..31263
                     /locus_tag="ACG9BE_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3031070.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            32274..32510
                     /locus_tag="ACG9BE_000282"
     CDS             32274..32510
                     /locus_tag="ACG9BE_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031071.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            32623..32865
                     /locus_tag="ACG9BE_000283"
                     /pseudo
     CDS             32623..32865
                     /locus_tag="ACG9BE_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32955..33194
                     /locus_tag="ACG9BE_000284"
     CDS             32955..33194
                     /locus_tag="ACG9BE_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031072.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            33341..37624
                     /locus_tag="ACG9BE_000285"
     CDS             33341..37624
                     /locus_tag="ACG9BE_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFN3031073.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            37723..38088
                     /locus_tag="ACG9BE_000286"
     CDS             37723..38088
                     /locus_tag="ACG9BE_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031074.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            38090..38542
                     /locus_tag="ACG9BE_000287"
     CDS             38090..38542
                     /locus_tag="ACG9BE_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031075.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            38991..40466
                     /locus_tag="ACG9BE_000288"
     CDS             38991..40466
                     /locus_tag="ACG9BE_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFN3031076.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            40489..40800
                     /locus_tag="ACG9BE_000289"
     CDS             40489..40800
                     /locus_tag="ACG9BE_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031077.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            41160..42416
                     /locus_tag="ACG9BE_000290"
     CDS             41160..42416
                     /locus_tag="ACG9BE_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN3031078.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            42413..42958
                     /locus_tag="ACG9BE_000291"
     CDS             42413..42958
                     /locus_tag="ACG9BE_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031079.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            42959..43459
                     /locus_tag="ACG9BE_000292"
     CDS             42959..43459
                     /locus_tag="ACG9BE_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031080.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            43818..44096
                     /locus_tag="ACG9BE_000293"
     CDS             43818..44096
                     /locus_tag="ACG9BE_000293"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031081.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            44116..44460
                     /locus_tag="ACG9BE_000294"
     CDS             44116..44460
                     /locus_tag="ACG9BE_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031082.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            44479..45390
                     /locus_tag="ACG9BE_000295"
     CDS             44479..45390
                     /locus_tag="ACG9BE_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN3031083.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            45469..46461
                     /locus_tag="ACG9BE_000296"
     CDS             45469..46461
                     /locus_tag="ACG9BE_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN3031084.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            46458..46676
                     /locus_tag="ACG9BE_000297"
     CDS             46458..46676
                     /locus_tag="ACG9BE_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFN3031085.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            46689..47099
                     /locus_tag="ACG9BE_000298"
     CDS             46689..47099
                     /locus_tag="ACG9BE_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFN3031086.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            47151..49679
                     /locus_tag="ACG9BE_000299"
     CDS             47151..49679
                     /locus_tag="ACG9BE_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3031087.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            49695..51905
                     /locus_tag="ACG9BE_000300"
     CDS             49695..51905
                     /locus_tag="ACG9BE_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN3031088.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            51913..53004
                     /locus_tag="ACG9BE_000301"
     CDS             51913..53004
                     /locus_tag="ACG9BE_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN3031089.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            53025..53567
                     /locus_tag="ACG9BE_000302"
     CDS             53025..53567
                     /locus_tag="ACG9BE_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFN3031090.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            53762..54019
                     /locus_tag="ACG9BE_000303"
     CDS             53762..54019
                     /locus_tag="ACG9BE_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031091.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            54033..54365
                     /locus_tag="ACG9BE_000304"
     CDS             54033..54365
                     /locus_tag="ACG9BE_000304"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031092.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            54410..54958
                     /locus_tag="ACG9BE_000305"
     CDS             54410..54958
                     /locus_tag="ACG9BE_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFN3031093.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            54965..55417
                     /locus_tag="ACG9BE_000306"
     CDS             54965..55417
                     /locus_tag="ACG9BE_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031094.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            55514..55855
                     /locus_tag="ACG9BE_000307"
     CDS             55514..55855
                     /locus_tag="ACG9BE_000307"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031095.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            56223..56414
                     /locus_tag="ACG9BE_000308"
                     /pseudo
     CDS             56223..56414
                     /locus_tag="ACG9BE_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT        19240 a         9125 c        12098 g        16033 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 cattaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    22861 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    22921 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    22981 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23041 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23101 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23161 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23221 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23281 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23341 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23401 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23461 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23521 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23581 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    23641 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    23701 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    23761 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    23821 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    23881 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    23941 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24001 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24061 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24121 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24181 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24241 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24301 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24361 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24421 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24481 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24541 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24601 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    24661 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    24721 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    24781 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    24841 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    24901 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    24961 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25021 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25081 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25141 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25201 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25261 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25321 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25381 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25441 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25501 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25561 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25621 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    25681 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    25741 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    25801 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    25861 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    25921 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    25981 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26041 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26101 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26161 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26221 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26281 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26341 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26401 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26461 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26521 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26581 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    26641 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    26701 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    26761 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    26821 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    26881 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    26941 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27001 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27061 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27121 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27181 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27241 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27301 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27361 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27421 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27481 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27541 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27601 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    27661 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    27721 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    27781 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    27841 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    27901 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    27961 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28021 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28081 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28141 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28201 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28261 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28321 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28381 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28441 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28501 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28561 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28621 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    28681 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    28741 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    28801 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    28861 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    28921 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    28981 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29041 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29101 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29161 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29221 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29281 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29341 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29401 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29461 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29521 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29581 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    29641 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    29701 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    29761 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    29821 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    29881 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    29941 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30001 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30061 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30121 gttctgaaaa tggatgagca agatagtgtg aaataactta taatcaatcg aaaaacctat
    30181 taaataaacc tttttgagta gatgcaacac tgaggattat ctgtataaaa gattttaaac
    30241 tgtcatttgg ttatcacggc gatactaaaa ttaacaaaat tggaatattt ttcttgaaga
    30301 tttttagctg tcagtatatg atgaaagcat ctcataggag gtgctttttt catggttgat
    30361 aattcaagtg gaagggttct taaatctcta agaaaagaga aaaaactatc tcaaaaaaag
    30421 ttagctgatt tagcaggtat ttcacaaagt actttagtta aatatgaaaa agggtcaaga
    30481 aaaatcccta aagacgtaga taatactcta agcaagattc tgaatataga aactctaata
    30541 aaagatgagg aagataagat tgagattctc atcggtaaac taattgcata cagagatatg
    30601 aataagttac ttaacaaaga gttagctgat aatataggaa ttagtgaagt attactcagt
    30661 tatgtgttaa atagaaagag aaatccgtca aaagaaatgc aaaaaaaaat cgatattttt
    30721 ctattaagta atgaaaaaga aattttgaag gagattaatc gtgatagtga gatatttagt
    30781 ttatcaaaag acgataaaat tgttatggga aaaagaatac gagaagtccg aaagaaccgg
    30841 gaagagacgc ttgaaaaatt tggtaaaaac tttaccatct atacaggcaa aaatgttata
    30901 agtagatggg aaaaaggtat taatattcca gatattgaaa aactaatgaa tattgcctat
    30961 cttggtaagg tcactgttcc ttaccttatg tacggagaag attataagaa tatacttcca
    31021 aaagatgaaa gagttagcga ttttaaaaag ataaattctt ttagtatggg attaaggatg
    31081 aggaaaatta ggaaagatta ttatctagaa agggaggagt ttggaaagct tttttctcct
    31141 tcaatttcaa aatggtccat agataggtat gagaatggta gagatatacc caatactaat
    31201 cgaattatcc aatatgctta tataggaaat ctttctttag aatttttgat ttacgggata
    31261 taaaaatagt ttacgtcgat acttattaag ttatcgacgt tttttattat agaaaaaatt
    31321 tataaaagga ggtttcttga tatgataaga aacaaaaagg gtttgttgaa agttgcttta
    31381 ctggcagctt ttttctaagt aactttttga caagctcaat ggcaacacaa gcggaagaag
    31441 taatacaaga actaactgtt atgaataaca gctagataaa atgggctatg tatgttatcc
    31501 aacaatttag taatcactag aagcctagcg aaagttcgct tgataagtta tagatgagta
    31561 ttgatttgta gtgtttccaa atcaaaaggg tacagagcca catgaaatat gttgtcctct
    31621 gcagcgtcag gatgggaatc cataccatgg agattcggag atctgtgagg gaatacttac
    31681 gtggttaata tgtcagttgt gttggacgta gtaatggtca tagtgggtag attgactgaa
    31741 ttcttatatg agaaaatgaa accaacaaga agatagggtt atttatacag aattggggat
    31801 cttttctgga aaagtaatcg gataatgcga gattcgtacg gtctttttta aatttctcta
    31861 aaaaaaacgg catcgaatgg gttgggtgat tgataagcaa tgaatcatag actgatatag
    31921 aataaaaaat gattctattt atctttttac atctatcgtt ttttcaagta gccgcaaatt
    31981 atatcagcat tcaacaattt ttgtatacaa gttctagtaa aaggatctga agaaaaagtt
    32041 ggttgttgtg aagataaaaa aagaatcagg ttttactgag tttttcttca aaatttgtgt
    32101 tattaggtta ggacaaagat aagaagaagt atgaagattt aaaaaaggaa tgaggaaaag
    32161 atagtaattt ccgattattg gagttttttt atttataaag aagaagcgaa taagttgtga
    32221 ttcctagatt tcgcgcgtga ttaattaatg agcaaaaaat gcggaaagtt ttagtgttta
    32281 atacacgagg acaaagtttc ttaggtattg aaggcacacg tgtaacggtt gatgggtggt
    32341 gtgattgttt cgaggttgga aaacatgttg aacttatggc atttactgag ctaaaagata
    32401 agaacaacgt ggaatttttc gagagtgata ttggttggga cgatcatgaa gaagtttatg
    32461 gaaaatttat tttgcggaag ggaaattctt atacgaatgg ggaagcataa gtagcgattt
    32521 gttagagatc attgatagga ttgaaattgt cgggaatatt tatgagaatc tagagttatt
    32581 tgaggtgata gaatgagtga tggttttgaa caagtatcaa taattaaaaa tcaagttcgg
    32641 gagattctta gaaaaaaagg ctatttggta gatagttatt ttgaaggaga ttacgaaaca
    32701 tgggtaggtg tctatgctcg tccagagaat aaacctacct atttagatcc tacgacgtca
    32761 gaagatgcct atttacaaaa taggtatcga gtagatggtt ttaagcaaga ctttgcggag
    32821 tggtttgaat gggaaattga aaacggagaa gtaaaagaag aataagagta cttgttgtat
    32881 caaaataagc cagtagaaca accaaaccag tagctagtaa tgacaataat actgaggaat
    32941 cggatcatta tggtatgatt gatatttctc ttccagaaga tcttaagcca gatcctgtaa
    33001 caattggcaa tagtgtcatg atttttgatt cattagatga agctatgtca tatggttgga
    33061 aagaaattaa ggatgaaggt agtaattggt atggcatgag atttggtgat ggtgaagttg
    33121 ccaaaaaagc tggtattcca attgggaaat ggacagttga tttccacgaa gcaggttctt
    33181 tttttgttct gtaattatag tgggagggta aaatcattga tgaaatgtgt tcagataaga
    33241 gcgccagata aaaatagaag agacagttca cgtcgttatc acttttggta acggcgtttt
    33301 gttttattat ataaaattct caggaaaagg aggaattctt gtgtatgcaa aaacgaaaat
    33361 gaattggttg aaagcactcg cattggtggc tttttctcaa ttctgtttct tggtgggaac
    33421 tgctcaagca catgcagaag aaacgacggc tatacaacct acgatggaag ccattgccca
    33481 agtcgcttct gaagtgacag aagtccaacc agcagaagcc ggggtcccag ctggtgaagc
    33541 cactgttgaa caggcccctg tgacagaagc gccaacggtc aattccaaaa cgactctcga
    33601 tcagacgatt ggggaaacac aacacccagg gaatagtcct ggtacttcac aaccagttga
    33661 agaaactgcc tcaattacaa cccctgcttc agaaacgtcc actctagaag gagaaaaagc
    33721 tgccgaagca acgccttctg tgcagccaga gtcttctgag aaacaagcgg aagaaaagaa
    33781 acgaatagaa gaaacagtgg aacaaccagc ggctactgct gacaaagaag gaaaaaatga
    33841 ggaaaacgtt gaaaaaacaa tctcttcaac cgcagctagt caaaatcaac caacgaaacc
    33901 aaacaaagaa tctacggcta gtattgaaaa tcaaaagaaa aaagagcaga acattccaaa
    33961 aacaggtaca cctacactgt tagtaacagt accaaccaat cgttcggttc gtgttcgccg
    34021 tgcaaccaat gcagctgttt taggtggtcg gaaaattgat tggggcgaaa atattggtgt
    34081 tacttctagt ccgaacaatc taaacttcac tttaaattat atggttcgtt ttgagattaa
    34141 tggacaagtg gcgttttgta ttgagcctgg tgtctcagcc gcagaaggtc aagtttatac
    34201 agctactgct ttgaaaaact atttgaaaaa ttcaaatgta cggaaaaaaa caagcttgat
    34261 ctcttattta ggatatgtca caaataaaga caaatccgat gaacaataca ttgcggcaca
    34321 atttatgatt tgggaagctt tgggcacaaa aatcccaatc aaaaatatcc gtgttgatta
    34381 tgatcgtcga aaagcagaaa ttcaacgttt gattaataac tttggtgaaa aagcaacttt
    34441 taataatcaa acacatacgg taaaaattgg cgaaacgttg aaagtaaaga ataaaaatga
    34501 ttttttgaaa tatgtgaaag acattcaaaa accaaaaggt gtcaatgtat ctattcacgg
    34561 caatgaagta tggattacag tcacaaaaga ggcaccggat aaagcaaaaa ttcaattgaa
    34621 taaaattaaa gttcaaggtg cgccattagc ctatcgtcgt ccaggagctc aaacaattgg
    34681 tgtgttacat ccaacagaac ctaatgcgtc tgttattaat ttacaaattc taaaacaagg
    34741 aaacattcgt gtgtttaaag aagatgcgga aactggtgtg aaagcacaag gtgcagcttc
    34801 tttagaaaat gcggtctatg gtttgtatca agcagatggg aaaaagatta aagaaattac
    34861 cttgaaaaaa attaacggca aagtacaggc agaaattaaa gatcttgatc ctggcaaata
    34921 tgttttaaaa gaaatcaaag cgccaaaagg ttatgtgctt tctgatgaag caattgaaat
    34981 tgttgttgaa cctggaaaaa ctgtcaatgc gaccgcaaaa gatcaagtga tcaaaggaac
    35041 cattgattta gtgaaagttg ctaataaaga tttagtcgat tcaacgaatc cagataacaa
    35101 accaaaatta gctggcattg aaatcacttt gacgtcaaaa acaactggca aagtggtgaa
    35161 aaccgttgtg acagacaatg atggttatgc tacttttggt aaaaataccg ttgtctttga
    35221 tacgtacatt ctttctgaaa ccaaaggaaa agaagggtat aaactatttg aaccacttga
    35281 agtcacaatt tctgaaaatg ggcaaacttt ccattatgtt ttagaagata aagtaattga
    35341 acaacgtctt aaagtggtga aagtggatca agaaacagga aaagtgattc cacttgctaa
    35401 tacagaattc aagatttttg atactttagc caatcgttat gtcacaatgg agattccaaa
    35461 tgatacagaa acaacggata tctttaaaac caatgaaaaa ggctttttcc taactaacgg
    35521 tacacttact tatggaaaag atcgctatcg tttggaagaa atcaaagccc ccgtaaatta
    35581 cgttttgaat caaaacccat tagtgttctc gattaccaat gatacagaac cgatcaaagt
    35641 gatccatttt gcgaaccgtc aagcacggaa aagtgtaaaa ttattcaagt atgaagaatg
    35701 gaacaagaag aaaacgccgc tcgcaggtgt gaactttacc ttgtataacg gtaaaaatca
    35761 agcaattggt gaatatacca ctgatcaaaa cggggaaatt ttagtgaaag gtctagttgc
    35821 cgatgactat tacttcttgg aaaatgcccc attagaaagt ttccaaccaa atacagataa
    35881 acaagcattc acggttgcat tcaaacaaaa tgcgccagat gatggggaaa cgttagtggt
    35941 ttctgtacgt aattacttga ttccaccaaa actagaaaca caagcgtcaa ataaagccga
    36001 tggtgaaaaa gtggttgatc cattagaaac gatcgtcatt aaagacagtg tttcttatac
    36061 caacttgttt gtcggaaaag aatacaccat ttcaggagta ttgatgaaca aggcaacgaa
    36121 tgcgccaatt ttacaagatg gcaaagaaat cacaagtacc ttggccttta ttgcagacaa
    36181 agtcaatggc acaaaagacc ttgaatttgt ggttaatgct agtgttctga aaggtcaaac
    36241 gatcgttgtt tttgaaaaac ttttccgtga tggcattgaa gttgcagcac atgcggatat
    36301 caacgatgaa gcccaaagtg tgaaagtgaa taatccaacc gttcatacca cggctgtctt
    36361 tgaagacgga ttgacagtcg cagacccaac aggattgttt accttaaacg ataccgttga
    36421 aattaccgag attcttccag gaaaagcata cttggtcaaa gggatcttaa tggataaagc
    36481 aaccggtgaa cccttgcttg tgcatggaaa tacggtagaa acgagtgttg gctttattgc
    36541 caaagcagaa aatgaaacgg tgatcgtacc atttaccttt gatttgacag gtataaatgg
    36601 tcgtgaaatc gtcgtgtttg aaagtctttt tagaaatgat gaagaactag caaaccataa
    36661 agacatcaac gacaaagggc aaacggtacg agtaactaac ccaagtgtac gtacaacggc
    36721 tacacataaa cccactggct tgaaagaggt taatccatta acgaaagtca ccattacaga
    36781 cgctgtgaaa taccacgatt tggtcgttgg aaaaacatac acagtcaacg gtgttctaat
    36841 ggacaaagta acggaaaaac cagtgctagt agatggaaaa gaagtcacaa gtaccttaac
    36901 gtttgtggca gaaacaacgg atggtacagt caatctagac tttattctag acgcacgtgc
    36961 gttgcgaggc aaagaagtcg tggtctttga agatctgttt agagaaggcg tattattggc
    37021 ttctcacgca gatattaccg acaatgacca aaccatcaaa atcagtaatc caaccattac
    37081 gacgaaagcg acgaataaaa atggcggaaa agagatcttg gccttgccaa atcaaactgt
    37141 gcttgaatgg gtcaaagtag acggtcttgc gattggtcaa atctatcgct taattgttca
    37201 aggctactta acaccagacg gcacaccatt tgaaggcact catgcggaaa aagtctttgc
    37261 ggccgataaa gaaatgatgg aattcttatt tgaattctta gtggacggtc gcaatcttgc
    37321 aggaaaaggc ttgagttttg cggaatggct ccaagcgaaa acggaagatg agaaagaaac
    37381 gtttgaagaa gtggcgaaac acaatgtaga tctaacaaac aaagatcaaa cggttcaaat
    37441 tgtggaagcg ccaaaaccac cgcaaccagt gaaaccagaa caacgggtta aaccagctac
    37501 tgttgtacca atgttgccaa aaacaggtga acaaacaagt agtatactta tggttcttgg
    37561 attagtagta ttagtcattg ctttaggtat aacactttat aaagtacgta aagccaaaga
    37621 ttagtcatga ggaacaagcg gttaggattt ttaaccgctt gtttgattgt aatgtagaaa
    37681 tatttagaat ttattttggt ttttaagagg aggaaaaact ttatggcaga aaaagcattg
    37741 cgtatgaaga aagagttttt aggtcaagta tttttattgg gttcacggga acctaatcca
    37801 ccaaaggaaa ttaatggtgt gtctgggcga tatgttcaat tacaagcaga gtgttcgcga
    37861 gaagaacagc ctgctttttt tgtttatctc acagctgaag aggataaaag attacgatta
    37921 ccagaaaaag aggtgcgtat ttcatttgaa gaagcttatt ttgtttttcg taaagaacga
    37981 gtgaatagtc gtgaaacaaa tgatatggga caaacagagc gtcgaacaac tggctatcaa
    38041 gatgttggtg tctttttaac tggtgtaaaa attggggagg ttttgtaaga tgggattaaa
    38101 atttccaaat aattatgaca ttacagaagc attagatgaa acttgtttag gagccgtacg
    38161 ctttggcgca ttagaagagt ctgatcgtgt atttgataat gaaacaggcg attatacggg
    38221 agaaattcga tcacagcgag cacaattagt ttttggaaat ccagatgaac caaagattgc
    38281 acgacgagtt aaatttccag cagatgtaga tttgactggt tttaaacgac gtgatcctgt
    38341 gaaattaatt gatccagaat atcatgtggt atatattcca gcacaagatg gcaatcgtga
    38401 atattcagat ttagtgattt atgcgaaagg attagagaaa gtagaagctc caacaaataa
    38461 tcctaatatg aaacaatcac aagaaaaagg tttagatcaa aaacaaccaa gtgttgattc
    38521 taaggaaaaa gagaaaaaat aaggtttttt tgaaagaata ggaaggagtc ttcctattct
    38581 ttttatctaa gatagaagga aggggagatt ttgaattcag ataaaaccaa tcaaatactt
    38641 tgagagattg tgcttgttat caaaatatcc aacaattgat tgagtatttt ggagacaatc
    38701 caacgatttt tgtacaaaca aatgcatata tttgtgcggt tattgggcag tgtatgtttt
    38761 tacgtgatct tgttcgtttt tcattaccgt accagtggaa acaagatcat caggaagtga
    38821 tttggattcg tatagtacga atatgtaata tgtgtgcaca cccttataaa aatcgattaa
    38881 atctttttcg attatgtttt tttactaaat gtgatattcc tgtattagtt gctttttgtc
    38941 aaaagttttt agcacaggaa aagtaaaaac agaaaggagg aatgtgacaa ttgaaagctc
    39001 attttttcaa gtatcgtggt cggcgaattc gctatttcaa tcgttattta tggttttgct
    39061 attatggttt ctttttgttt ccatttttga ttctgttagg ttggtttagt tggaaagtat
    39121 tttatccacg tttaagtacg ttttcgaatt ggcaaatgta tcttattcct ggaataagta
    39181 ttgtgttgta tttgttgctg gttagtgggt taattttagg gttgatgcat tggtcgcgac
    39241 taaaagaagg atattttgcc tctgtttatt ggcgtcagtt acttgttcga atgttaattg
    39301 acaatcggtt tgtttataca aagaaacaag ttagtgagaa acaaacaagg gaaaaaatga
    39361 gattaccaaa agtttatttt tatcaaaaga aacaagaatt agtggtttct tttcctttag
    39421 atggtgggcg ttttcacgaa cgatttttaa aaatgggtca tttgttatct gaagtatttt
    39481 tgcgtgattt aattcgtgaa gaacgagaaa aagcccgaat caattatgta ttcctttcag
    39541 attctggtcg gattcctatt gatcaatgta tagcagaaaa tggacgtatt catttaatga
    39601 aaactttgga ttgggtatat gatcaatcac caaatatgtt gattgctggt gcaatcggtg
    39661 gtgggaaaac ctatttattg tattctttaa ttcaagcttg tttaagtgtt gggacagtag
    39721 atatttgcga tggaaaagcg gcagatttag cagcattagg tgacgtcgat gtattcaaag
    39781 gacatgtgtt ttacaaaata aatgaagaaa tgattatttg tttacgaaat gcattaaaag
    39841 aaatgaatca acggtatgcc tatatgaaaa ccatgaaaga tcctaaatat gaaccaggga
    39901 aaaattatgc ttattatggg ttaccaccgc attttatctt ttttgatgaa tgggcagctt
    39961 tttatggttc gttggatatg gaagcgagaa atagagtcga taaattgatt cagccattag
    40021 tattaaaggg tcgtcaagca gggatgtaca ttattttagc aatgcaacga ccagatgcag
    40081 aatattttcc aagtggtgtt cgggataatt taacattccg tatttctgtt ggtcgattgt
    40141 ctcccattgg ttatttaatg acttttggag aagattttaa aaataagcca ttttacaaca
    40201 aagatcaacc tggtcgtgga tatgcttttc caggatttga tgtacctgta gagttttttg
    40261 cgccatttgt ttacaaagat tatcacttta tcaaagaatt tgagaaattt actgaaatga
    40321 ttgtctatga ttttacagta aaagaggata gtcaagatca aacgatggga gatgataatc
    40381 agttgttaca agaacaacta gagcgccaaa tgtatttagc aaaagatcca aaaggaacaa
    40441 gtttttctga tgcgcatatt ttgtaatcaa ctaaggaggt gaagttaaat ggcagagagt
    40501 tgttattata gagtagctac agcaatacga atgattaatc cttctctttc ttccagaacg
    40561 ttttatgatt ggttgaatcg tattgaacaa gtaactgatt accgcttttt gcgcaaagaa
    40621 cgtgttttta caggaaaagt tattaatcaa gtattgctaa cgaaaaaaga tatagaacga
    40681 ttgactcgat tgtatcatta tcgagtagat ttagaagagg atttaacttt gtcaatttat
    40741 cgcgtttttt ctcctgaaaa atatagtgaa ataacaaaat tagatcattt aattttgtag
    40801 ttattatcta tcgggagggc tctagacgta gtctggtagc cgcactctgc ctggcggtgc
    40861 gcattaaggc tgcgaagcat gggcctataa tgcgcactcg ctagcagcgt gtgcagctca
    40921 caaagaggtc gtgcgcttgc gcacggcctc tttgtgtaaa gccccccgat atataatatg
    40981 gggggcagaa ataacataac acatagaaaa aagttttata aacccttttg tatcaagtga
    41041 ttatctaagg tcagcagttg tactactttt cccaaaaaac gagggggact agttgatata
    41101 tcggtgtttc taaagggtta aaaaaatcga ttttgttatt ttataaaagg agcgagaaaa
    41161 tgacaccgat tgagctaaaa agctacagaa agaagttaag tgcaattcat aatataagtg
    41221 gagataaaaa gccgctatca caagcaagat ttgcgcaatt gatgggctgc tcaaaagatt
    41281 acattagaaa gatggaacaa gggaaaagaa aaatcccaga ggattttgaa caacgaatca
    41341 aggaacgctt tcgattgtat atgaaaagcg gaatagatct tcgagcaatg attgattatt
    41401 tgcgtctttc cttttttggg acagactaca aaacagtgat tcaacgtgta ttacgaatgg
    41461 atctgacaga atttgaaaca cgtccaacgg gcttgtatct ttttgactgt ctttctgttc
    41521 gtgggaatat ctgggtgttc tggcattcaa aacgaactgt aacagaaaat gtacttgtgc
    41581 aattttcagg acaaggttgc cgggaatatg aggaagtcat gcgtgaacaa caaacagatt
    41641 ggcgaacatg gctatattcc atttggaaaa atcaaggaaa gttggaagct ggttatacac
    41701 gtgtgcaatg tagccgattc gatattgcgt tagatgaact ttggggattg gaaacggaag
    41761 aacatttcga tctctttgaa ttactagaaa aacaaaaagc aggtttatta gaaatggact
    41821 ttaagagatt ccagaatatt ggcggttgtt ttttagatga tggctatttt cgaagtgaag
    41881 gattatcttt gtattttgga tcgcgaaaga gtcctctctt ttttaatttt tatgagaaac
    41941 gttttgaaat tgcgaaccga gaaaaaattt cagaattaga agcattaacg aaatatggta
    42001 tttataatcg atatgagtta cggttagcca atgagaaagc aacacaagca gtagaagcat
    42061 ttattttagg tgattcaccg aaacgtttag gagagattgg cgttggttta attaatgcaa
    42121 aacttattgt ttgggataga ctggaaagtg atgcacggat tgttgataaa cgatgggcaa
    42181 aattatttcg ttcgatggaa gaattggatt ttacagtaaa gccagaaaaa ttctcattag
    42241 atcgttcaga acgctggttt ttgaaacaag tagctccgac attaaagttg ttacaagaag
    42301 cagatcgtat tttaggaaga gactttgtac aagaagggat tgataatgcc gaactaaatg
    42361 acgagcaaca aaactatcta gaatggatgg gaacgtcagg agggaaaatt gcatgaatgc
    42421 atatacgaca acagaatggc tgcgcctgtt ggatttaaag gctgcaatcg aagcattaaa
    42481 tgaaaagatg gttgatttgt cttattttcg ttttcgagtt ccttatattg aacaagcggt
    42541 aaaagctgga cgttatcagg aaaaagagaa ctggcaagaa attgcacgct tattagaagt
    42601 tcgtaaggga tacgaacaag aactcgaaga gttggaattt agccgaagga aaggaaggct
    42661 agaattcatt cgcttttatc gattttcatt gccaatacct gcgattttag cggttaaaaa
    42721 aggctgcgat aaaatgaaga tatatgaaaa ctgtgtggca gcacttagca gcgaaaaacc
    42781 tcttatggaa gaaatttcat tggtgactgt cacttgggaa atgagtcgcc agccaacaga
    42841 agaacagaca tatctttctt tagaagagat tgagatagaa ttagaggaaa ttggtcgcta
    42901 tgccacttgt tctacctatt gtggatcggt cattagtatt gctggtgtga ttgtctaaat
    42961 gaaaaaaatt atgatttatg ttggtgcgta ttggtcacgt gatccaacag tattagaaaa
    43021 tccagaggcg gtacactatt gcttgcgcca attattctat ctgtataaag aacgattaga
    43081 atcgttgatt cgtcagttac catatactga cagacgttta gatgaacttc ttctaaggta
    43141 tccagcaatg tataaacgac gaaaaaatag attgttacca gaagagtatc ccattgaaaa
    43201 acatgaactt gaagggcgat ttgtggctta tttctatgat gatgttcgta tgcgcttagt
    43261 agaacaacga atggaaatag aaaatgatcg ctactatttt atcaattatt gtaagaagcg
    43321 taagtatcaa gtaactgatg atttttatga ttgtatttta caagatgggg aagtgattct
    43381 atccaaaatt gctccaagtt ttcgtgaact tgtgccgatt gattttttga aaaaatgtca
    43441 tttgcggatt cttccgtaaa aaagagctat aaaaggaaag aaaagtagaa gccaaatcgg
    43501 tttctgcttt tttatttttt ttaggaagga gaagatggta tgtcactgga atttttagga
    43561 gagccaattg aaaaggaaga gttcgtttcg cattatatgg atttgtttat tcaggtgata
    43621 aaaaactttg tgcgattgat gaattttatc cacgcgaaca agactatcta gccgctgaac
    43681 gaataaaaat cagtttatta tgggaaaaat tcacagatga aaataaccga ccgccagatt
    43741 atcgctattt atcagattgt ttaacgaata atgtgctttc taaagaatac ctatttcaca
    43801 aacgtgagaa aaatcaaatg tcggttgaac atttttgtga ttgttatatt gcattattgc
    43861 gtagtcaaca gttattaaca gtagaacgat atggtgcaga gtttggttat gtcttgcaag
    43921 ctgagcgtca tagagctaca agatgctatg aaaaagaaga tcaagtgatt gatggctatg
    43981 aagaattatg tattcgaaac aatcgctatt tgcaacaacg tttatatgaa caattgatga
    44041 tggatttttc aaatctctat agagggtatg aatgttcgat gattcaagaa aggtagaaga
    44101 aaagggggaa tttttatgtg gcgattatta tttttggtgg tatgtggtat ggcattgttc
    44161 tatgtgtatc gtgtctatct ctatccacga atggaacgat ggacaagttg gtatagagat
    44221 cctttaaaac aacgaaaagg attgaagcgg tatcgttatg aattacttgt tgaattttcg
    44281 gatgggcgac aagcacagta ttattttttt gcaaactatc caaaatacaa caccagtgaa
    44341 ctattgattc aattattcaa aaataatcca tatgttgggt tagaagaaaa cggacgggag
    44401 ttcttttatc gttcagataa tattagtgcg gcgtctatga acaaaacacg aattgcataa
    44461 aaaattggag gaataatcat gaaaaaaaca acattagcag tcgtaacaac actaggaatt
    44521 tcattatttt cgtttcaatt agggagttca ttggtttatg cagatgaaac tgtagaaaca
    44581 cctaagtcag aaattgtaga aacattgcca ggggaaggat taccagatgt tgagacagag
    44641 ctaaatggag gaacaacgga aaaaccagca gaacctgaac aattgcagtc agagaaacct
    44701 gaaaaagagc cagtgacacc agaaccagag tttcctgaat caactttacc gactgattcg
    44761 atcgaagaag aaggtgtgca tagttcaaca acaacggatt caagtatagt cacaactccg
    44821 gaacaatcag gaacaaccag ttcttctgaa aagaatactg attcgtcaag tacggaacag
    44881 ccaacgatgt cgtctaccag tgaagaacaa aaagaagaac cgaaacaacc tgttgaaaaa
    44941 tcagaagcaa ttccaaaaga aaataaacct attgtttcag caaaagaagt cacagtatcg
    45001 gtaaccccaa gcggtgaaat tacgacgaat acttctcaag gaatgagtgt accgattgtc
    45061 actagtaatg tagaggaatt gacccatatt ccaacaccaa ccaccccatt aaaagtagca
    45121 ggtgatcaaa caattgttgg tgtaaaagat ggtattccat taatccaaga tagtgaaggg
    45181 aatttggtaa aggatttgtc gattcctgta aagaaacttc caagtggcaa tattgaaatc
    45241 aaaacagcag atggaaaaac aaaagtctta ccaaagactg gtgaagaaat acatgttgca
    45301 ctttctgttc taggaggagt gttgacaagt attgctggat ttgttggata taaaagaaag
    45361 agaaaaggaa tagaaaatga gggtaattga atggactatt tttcagaaaa acaagcttat
    45421 cgttgtcaat cgtacgtact agtaaaaaca tactagaagg agtagcttat gaaaaaacga
    45481 caactacggc ggaaagaaaa agtttataca gagaaagtac cgaagcaaaa gaaaatccat
    45541 tcaaaaaaag cagtgaaatg gatttggtgt ttggttttat ttctttgttt ttcagggggc
    45601 ttggcatttt ttcaatccat gaaaacgaag gttcaagtgt cgtttttgaa agatgaaatc
    45661 caacaattac aaaagaaaga agtacaaagt cggtcagaat taacgtacac acctgaaatt
    45721 gaatccttta tgaatcgttt tgttgctttg tatatgaata cattcgataa tcatgaacaa
    45781 gaacaacaac gtcaagaaac attaaaaaaa gagttttatg cacaaaactt tattcaagaa
    45841 ccaggaagtg tttcaggtgt tcgaaaatta actgctgctt cgtttattgg attaaaagaa
    45901 attgaaacca ttccaactgc tagttataaa gtaacttatg atattcgctc gatgaatcca
    45961 gagagtaaga aagaaacaag tgtgacaatc acacaagtac tcaatcttcc cttttctatt
    46021 tcaaacaagg gctgtcgtat tattgcttat ccttatttta cggcactacc aaagaataca
    46081 gaaaaacaac gaatgattac ttatgatgac acattatacg aaacggttga tgcgtcggaa
    46141 gcgaaagatg ttgaaggatt tgtggaagat ttcttagata agtatgcgaa aaacaatgca
    46201 aaagatatgt catacatgat gaaagaacct gaaggactca atcaaacagc taaagttgat
    46261 caagtagaga tgaaagtatt gcgttataaa gaaacgaaaa aaccttttat tgtaaaagca
    46321 acggtcgtct ttcgagatgg tgatacgtca cttttacaaa aagaacaaat gacgattgtc
    46381 attacgaaaa aaacaaatca gtattatgtt gaaaaattaa cacataattg gttggatagg
    46441 aaaggagaag aacggaaatg acactgaagg gaattgtaga ttttatatta acgcaaggtg
    46501 gctatttttt aataatggtg gttgtttatc tgggattgcg aaattttatg aaatcaaaag
    46561 taggaaatgc attaatatct atcgcagcag gtgccgcagt ttatttcttc ttaaatgatc
    46621 caacacgtgt atttgatgcg tttggtaaaa tcattgaaaa attatttatg gattaggagg
    46681 atagtgaagt ggattgggaa ccttatgatt attcgattgt ttatgcggaa ccttatcgcg
    46741 tacaacagat tacaagcaat tggcgtcttc catttccagt tgcattagtc aatgcagcaa
    46801 cttttttgtt ggtcgtaatc tttgaatatt tatttttacg ttcatttatt ggttgggtca
    46861 tttctttttt tccaccagca ggagttgttt tttatatagg actcccctat ttatgtgtgt
    46921 tagcactggc aaaactaaaa cctgatggaa aaaaagtgca tttttattta tgggattggt
    46981 tttgttatat gattcgtatg atgaatggtg attgtgtgta ttataactgg gacaaggtat
    47041 caaacaatgc attggaagaa ttactttttg tgaataagta tgtttattta gaggattaga
    47101 aaaagggaca atcaactggt tgttcctttt ttattgggag gtgaaattca ttggtgcttg
    47161 taaaagaacc agtaaaagca ttcaagaaaa attgtttgtt aaatggagag gattgtatct
    47221 gggcctacta tcatgttttt ccacgaacaa tttcaaaaaa cacaccaaaa aagacacagg
    47281 aaataaaaga aatgatggct cgtttactgt tgcttttagc agattatgaa gatgtggagt
    47341 tggtgatgta tccacgaaca atgaatttac ggacgaagtt tgacacatta ggaaagggat
    47401 tttctcccga tagtcaagca attggtgcgt attatgcaaa agaaacagtg acattgttgg
    47461 aacgagaagg tcaagtctat cgtccttcat ttattttagg aatcaaagtc aaagaaaaag
    47521 caacggtgga gagttttcat gatgtaataa atctaatctg gttagattta gcaggaagtt
    47581 tagcacgctt atttggatta gaagtagatg atttggaaac tttgttttct gaaagtgatc
    47641 cttatgtaaa agaagtgaca caattacttt atagcttttc tattcgtccc ttagtaaaaa
    47701 aagagctgtt gtatctcaat cgtctgcaat atattcgagg aatggctcac aaagtagaag
    47761 aacaaaataa ttgggaagta ctgaaagaca atcaaggaac gtatgtacgt ataccgaagg
    47821 aacataaagg ccttcttcaa ttagaagatg agttagggag tagttattta gcattgttgc
    47881 ctattgggga aacacctgaa aatatgagaa attgtgatct atttcaattt gctcaggatc
    47941 tttctttccc agcagaattg cgaatgaaaa ttcactatcc acgcttgaaa ggacagggag
    48001 gtattggttt aaaactgaat tggctatcgg aacggttcaa aacagagcat aaggaattgg
    48061 ttcaaaatga tgaagaacct agtgaacgct tattaactgt tcgaggatta gtaaaacatt
    48121 taaagcaacg agtagataat gggcaacctg tcgtcgattg gatcgcatgt tttattgtat
    48181 acggtcaaac ggttgaagaa gttcgtcaaa atagtgcaac ggttattcaa caacttcata
    48241 cgattatcca tatccaccgt gcaaaaaatg atcaacgaga attgttctat aaatgtttat
    48301 tagggcaacc attgggacgg cagttacatt ggaagcaccg aacaactgta gaagcagttg
    48361 gcgaatggtt gttttcagca gtatcagaat taggtatgga atccggctgg tatattggtc
    48421 ggcaagatac attaggggat ggtggttctc gaggagaaaa aacaccatta gaagaattaa
    48481 tttatgcgtc aaatcgtttt gtctttttga atcttctagc cattgcggaa ggaatcaaag
    48541 gtgcgttgtt tgatgcgcct catgtagcag taactggaaa aaccggtaaa gggaagacct
    48601 ttttagttgg cttgctcttt ttgtatagtt cgtttatgaa tgtacaaagt ttatttgtgg
    48661 atcccaaagg agaaaagcgc aaatggttta cgaaactcat tcaagacccg tattatcaga
    48721 aacattaccc tttgtttgta gaacatttgc aaaaaatgag ttatgtcacg ttagatgcta
    48781 caaatccccg taattatggt gtattagatc caattgtttg tttatcccga gtagatgcga
    48841 aacaagtagc ccaggatatg attgatgagt taagtccgtt aggtaccaat caccttttaa
    48901 aaggggcggt attaaaggga atccaagaag ttttagttca aaaagaagcc ggacaacaag
    48961 ttggcttgat gcatgtggtc gactatttag aacaaatgga acaaaaagag gttcgagaat
    49021 atggtgagtt tttacgcttg acggtagaag attcgatttt acgtttagga ttttcttatg
    49081 gagaaaaccc gggattagac tttaatgcca aaacaacgat tttagagatc caagatttga
    49141 aattaccaaa tgacaatgtc cgaccagaac tatatacaga tgcggatcga aagtctttgt
    49201 gtttgatgat tagtttaggt cgtttttgtg aaatgtttgg aaaacgagat agtagtaaga
    49261 aaacagcgat ttattttaca gaagcatggg tatttaataa ttcgaacgca ggacgtagta
    49321 ttattgcagc aatggcacgt gtggggcgtt cccagatgaa tcaattagtg ttagatacac
    49381 aatttattgg tgacttaggt tcagataaag aaaaagggaa ttttggtgtc gtctttgctt
    49441 ttgatgaaga cagtgagcga gaaaagattt tgaatcattt acgtttggaa gtaaatgaag
    49501 cgaacttagc tgaaatgagc cacatgatta aaggacagtg ttggatgtta gatccctatg
    49561 gacgtgtagg aaaactcaat gtacacagtt tgtttgaaga aatgaccgct gcattacaaa
    49621 caacggaaaa aacaagtcac tctcaagtag aagaacagga tacgttaatc atgcaataga
    49681 aatgaggtga aacaatggct tatataaagc ataaggaaaa gaaatggtgc gttttgtttt
    49741 ttttaacgat tggattcgga ctgctttttg ttggtggatt ggtgtatgcg gcagaaaagc
    49801 aggtggatca atcgatctat gagattggga aatttcatat tgataccttt tcttcttatt
    49861 tatctgatag tgaattttca ttaggcaaaa tgttatttgg ggcaaatggc acagattttc
    49921 taaaaatgat gacgaatatg ttctttagtt taagtaaatt gatttggcaa ggccttgatt
    49981 ttgtcattga gaagctctat gaaggagctg caatgaacga gttaattcac cagtttttca
    50041 ctttttcgga tactatttat tcaaaaatat acgatcctat tggtgcgggg attgtcgtct
    50101 tgtatgtgtt atatctattt actcttcgta tatttagagg gaaacattac gcaaaaatgg
    50161 tgtttattcg atttctcttc gttgtcattt tttcaatggt ttggtttggc tatggtagta
    50221 acgtacctag taaaggagaa gcttttacca aagggttaaa tgatttgtcc gtggaggtag
    50281 aaggctttat tttttctgct acaaatagtc tagatgaatt ggcgacaaaa gatgtcaatg
    50341 gtgttttagt gccaacgaca caaaaggaag ccattcaaaa agtgcgccaa ctttattata
    50401 aagcggctgt tgttgatccg tatatattgt taaattatgg gacaacaaat ttagaagaat
    50461 tgtatcaagc agagattgaa ccacaagaat ttctagccaa ggaagcgtca agtgaagaat
    50521 tatctgatat tgagacaaaa gtgaccactg ctgcaggaga agatagaggg aaaaagaagg
    50581 cttatcgtgt ctatattcaa ccaactaaag ctgtttataa agcagtcgtt ggttctatgg
    50641 tgccattgct taatctaagt attggattac cacttgtagt gattggcatg attcgtttta
    50701 tctttcaaat gttggtgttg tgtttattac ttgtgattcc tttttcatta ctcgcttctt
    50761 ttgttccagt atttgattat atgctcatga ataccttaaa gtcctttttg ggcttgatgt
    50821 ttcaaaaatc tttttacagt gtgattattt tggtggcatt tttgatctat aacatagtag
    50881 acgatttaat tccaatgaat actgtttttg gttttttagg aaatatgttt gtcaaagcac
    50941 tcctatcaat tttactcatt gtaaaacgga aagatttatt aaagaaacta gggttaggag
    51001 ctgcagatca aactataaac aacacgaaaa acatagcaag tcattctttg tctcaaatga
    51061 aacaactgcc tggtcaacta tcaactgctg gacaaaattt ggcaatcaaa ggtgcaggat
    51121 tagcagggaa tgtgtatcga gcaacgaatc cactcgcttc tgcagcgata catggatttc
    51181 aaaagtttca acaaaaacgt acaccacaag aatcggtaaa agggaggcaa gtggttaatc
    51241 cctttgaact aggtgggcgt tcaaatcaag ccttgcctta tagtggtcgt catgttcgtt
    51301 atcatggggt agagcctgaa atgtcttcta ccaaatcgaa aacagagtgg aattctggtc
    51361 tttcaaatgg tttgattcca aattcgtcca tgaattcagt aagaccaaaa caacaggtac
    51421 aatcattggc acaattgagt aagcaacaag ggaataatca aagaaaacca ttgttaaata
    51481 aaggtatcgc ttttgttggt caatcgattc cgttgccttc tggaaaagta cccgtgccac
    51541 aattaacagc tgctggttct ctttcacaac agattcgcca agtgaaaaat caggcgaaaa
    51601 atgggagtta tccagcaatg cagcagttat tgaaccaata ctctgttcct aattcgtcgg
    51661 tagccactct atcaggaaat cgttctgtgc aacaaccggg gcaacgttcg tctgaaagta
    51721 aaccgtcgat ttccgcacca aaatcgttac aacctgcatt ttcttcttta gataagccaa
    51781 cagagcaatc ggaaaaaata gaaaaggatc ctaaaacact gccgattttg gacgcaaaac
    51841 caggaactga ttttacttta ccaaaaggac gaacagtcca aaaaccatta tcgaagaaag
    51901 gatgaggaag aaatgaagaa agggtgtcta ggttgtcttc ttttctttat tcttcccttt
    51961 tttctattgc cattcttttt ttcaggagaa ctttctgagc atggaggaga agctacaggt
    52021 ctagctgtcg cttttaatgg ggaatatacc gaaggattac cgattttttc agagattaaa
    52081 ggacgtggtc caatcacaga tgaacatgcg caagcagcag ttggtgcagc tgtaaagtat
    52141 cagttattac cgagtgtgat tctttctcaa ttaggctatg aatcagctta tggacagtca
    52201 caatctggaa gaaacgatac gaactttttt gggattacct ggtttcctgg ttgtccctac
    52261 ccacaaggtt ctgcacgtgg aattggtggt tctgaaggtg gtttctatat gaagtttccg
    52321 aacttaaaag cttgttatga ctattatggc tttatggtgg cgagccaaag caactttaat
    52381 caatgtgtgg gcaataaaga tcctggaagt tgtttattga ttctaggtcg tggaggctat
    52441 gcggcagcag gtattaccga agggagtccg tattatcgag gagcaatgag tatcataacg
    52501 tcctataact taacggaata tgatactttt gcgatcaagc attggaatag cctaccaaca
    52561 aaaaaaactt cgcttattgc aggaaaaagt aaaggcaatg cgaaggtttt ggatcggatc
    52621 attggacgat ccgtaaataa tggggaatgt tatggtggta cggcatggta tgtatctcaa
    52681 ttaggtggcc cacagttaat gggttctgga cacatgtttg cgatgttaat aggggaagat
    52741 tataactgga caacctatgg ttggcaagta atcatgaacc caaaaccaag tgatttacaa
    52801 gcaggagatg tggtgaattg gcaagcaggt ggtgcattgt ctcctggaat ctatggtcac
    52861 accggaatta taacagatgt ttcatatggc ggacaacatt tctctagtgt ggaacagaat
    52921 gccgagcaag gacggattat ggctcggtat caacgcacgt acaatcaaac aaaaattcgt
    52981 agtctggttc gtaaggtgaa ataagaaagg cagtgactag aaacgtgaaa aaagggataa
    53041 ttttagtatt tagttggtta tgtttgatag gtcttactgg ttgtcattgg ttttcttctg
    53101 aaaaacaaaa aacacaattg agaacgacag aagaaacaat cgaagcagta aaagaaacaa
    53161 gtggaactct tcaaagtact gaaagtaaca gtcaaattgg ttcattagaa gacgagacag
    53221 cgcaacaaat gcaaatagat gaaatgacaa aaaatttctt aaaagatttt gctagaaaat
    53281 ggagtaattt cacagatgtt tataaacgca atcaagctgt gagggactat atgacagatc
    53341 gttgtattaa agacaatagt attgatgtag acccgcatgt acaaatgaaa acaactggga
    53401 aactttacca ggtgacacaa gatatagaga atccggatca atatttgtta tttggagaag
    53461 aaacaatgaa taaggttact caatttgtat tacttcaatt gaaagtaggt caagaagctc
    53521 aaaagattga tgcgatcaag atttattatg tacggagtgc gtattaaaat aaaaaatgaa
    53581 cactcatatt tattatacac cgaagaaata gaaaaaaaca gactgttttt ttaagaaaaa
    53641 ctatgtcatt ctttacttga aaaaagttgt tataggacag aaaggataaa acaaaaacgg
    53701 aaaaaatatg tcttatgaat aactataaga actattctat tttttaggag atggatacga
    53761 gatgaaaaaa ctaattggct atagtgtgat gtgtattgga tttgttggtg tgggattgtt
    53821 tgtatcgact gaatctgctt ctgcggctga atggtataat cctatctggt ataatccgaa
    53881 cattgaatgt caaaaaggct atccgtatgg accgacaaat ctgaaaaatg gaaaatgtag
    53941 agtaaattgg aatggcgttg tagatagtgt aattactggt tcaatggact ctgctctaca
    54001 tggctttacg ggtcactaga aggaggaaga aaatgtctga agagaaaaaa gaaatcgttg
    54061 aatcattagt agctttaaaa gattctcttt ctaagattga atatgttgat aggcaagaaa
    54121 ttcaaaaact aattgatgat actattattg aaatccaaga tgcgcgttgt gaaggtataa
    54181 aaatttctgt agctttatct aaagtaatag aaaaaatgaa ccgttcatta gcgtttaatg
    54241 gattgaagct agatagacaa actagtctga tatgggatca tttaaaagac ttgtatgaca
    54301 agtctaaaag aagtgaacgg actgctgtat caatcttaaa aggcctttgg ggaatgaatt
    54361 cataaacaag atatttaaaa agtaacttat tttttatgga ggagaaatta tgaaaagaat
    54421 aattaaaata tttggtgttt taggtgtttt cttattaggt ttaagtactt ttgttagtac
    54481 tggaacaagt gtgtatgcca cagaaaaagt gaatacagtt gatttagaaa taaagaataa
    54541 attagtagat gaattaactt tttattttga tacagttggt tttttagacg aacatggtgt
    54601 ataccatata aaggattttg gtgctattcg tgaacgaatc agtcaagata acgaacaagg
    54661 aagagcagca agagtctttt atgaacatat tattgttcct agaatggaga aagttgattg
    54721 gaaacgatat gctggatgtg tagccattaa ttctcttcct ggaggaggag tggcttgggc
    54781 tataggtacg cagggagatt ttattgaagc attaaaagct tttaattttg aaaaagctgc
    54841 ggatttatta ttaaaaattg cagaaaaaca attaggaaaa gctgcagcta aagagttagc
    54901 taaggtaaat attgtagcag gaattgcagc agctgctgct agctgtgcat tagagtaaaa
    54961 gtttatgaaa agtttagaac aagagcatat ggcttttaaa caagcaatgt ttaaagaaaa
    55021 tatctattta aaccataact atattcgtgt ttctaaggca tgttcaccag tattgaatat
    55081 gcttggaggt ggaaatggat tatatcattt actgtttgtc gatgtttgtt ggttaatttt
    55141 tttaccagat gaactagtta tagtcaatga aaaaataaca agtaaaaatg aagtgtttaa
    55201 ttattcttta acaagaatta attacaaaga gattaccaag ttttcactag aaaaagttcc
    55261 tttttgggga gaattttgtt taaaaattaa atgtaactgg aaaagaatgt atttctatat
    55321 tgatggtgat gatgctctta cattcggtaa aactactttt tctagtttta attttcagtt
    55381 tctattaaaa aataattttt atggtttatt gaaataaggt tgcgaaggaa gatgtagagt
    55441 tatttgcact gttgtgttgg tatatctctt aatgctgtgt ctttttgggg agattaatta
    55501 gtggagtgat tatatgagta tagatgattg tttagaagaa caaataagat tatttttaaa
    55561 gtttttaaaa gttaaagttc aaaataacta cttcacatgt tatagttatg cacaccctgt
    55621 tttaaatagt cttcctttta attctttagt gcaaggattg attacaaata ttgttttatt
    55681 agtctgtaca ccaaatgaaa tatttgtaaa aaagataggc agtggtattt cttcagtaag
    55741 attaaaagag aaagaatata ctcaaaagat tattcatatt cctaagcctg aaattaagcg
    55801 ttttgagata aaagactatt ctgctttagg gctgttctca attggttact ggtgataaaa
    55861 aataagaata ctaaaactat acgtagaata taagtaatcg gcatcaatcc taattatgtg
    55921 attgaatgcc gatttttgtt acgcgctatt tttaattatc tgtaagaaga ataattttat
    55981 tttactcata ttcgttttca ctttgaagaa gtaaattgca atttcttata agttaagcag
    56041 ctatgcaaaa ggctatttat tctattgtga aatataagat gtagttgaaa caggatatat
    56101 agaatcaaaa tacgaatctt attttgattt ttctagtttt ttgaaaaaag caccctttga
    56161 tcgttgtatt agtggaaaag agaaaaagga gcatacttat catgtcacaa aactacaact
    56221 aaatatctaa acaacaccaa tttgatgcct attgaaaaaa agtattgcga aatgaagcca
    56281 agtctattag aaaatgaaat aatagaataa aagaaaatgg gaaaccgctg aatgatgtaa
    56341 atgaaggtaa gtatccacga actaattttg atgaacaaga tatctatttt ttatttgtat
    56401 tggagatact agtttaatat caaaagttat cagcagtcat aattagttat ccaatgtaaa
    56461 aagtacaaac aatagttgtt atgcagaata gtttat
//
LOCUS       DAZAID010000006        53481 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000006 DAZAID010000000
VERSION     DAZAID010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.006"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            547..1275
                     /locus_tag="ACG9BE_000309"
     CDS             547..1275
                     /locus_tag="ACG9BE_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3031096.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
     gene            1293..2486
                     /locus_tag="ACG9BE_000310"
     CDS             1293..2486
                     /locus_tag="ACG9BE_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN3031097.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            2479..3474
                     /gene="lacD"
                     /locus_tag="ACG9BE_000311"
     CDS             2479..3474
                     /gene="lacD"
                     /locus_tag="ACG9BE_000311"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFN3031098.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            3471..4412
                     /locus_tag="ACG9BE_000312"
     CDS             3471..4412
                     /locus_tag="ACG9BE_000312"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFN3031099.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            4458..6224
                     /locus_tag="ACG9BE_000313"
     CDS             4458..6224
                     /locus_tag="ACG9BE_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFN3031100.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            6241..6720
                     /locus_tag="ACG9BE_000314"
     CDS             6241..6720
                     /locus_tag="ACG9BE_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN3031101.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            6733..7623
                     /locus_tag="ACG9BE_000315"
     CDS             6733..7623
                     /locus_tag="ACG9BE_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN3031102.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            7610..8419
                     /locus_tag="ACG9BE_000316"
     CDS             7610..8419
                     /locus_tag="ACG9BE_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3031103.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            8435..8839
                     /locus_tag="ACG9BE_000317"
     CDS             8435..8839
                     /locus_tag="ACG9BE_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3031104.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(9020..9958)
                     /gene="yidC"
                     /locus_tag="ACG9BE_000318"
     CDS             complement(9020..9958)
                     /gene="yidC"
                     /locus_tag="ACG9BE_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFN3031105.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(10292..11374)
                     /locus_tag="ACG9BE_000319"
     CDS             complement(10292..11374)
                     /locus_tag="ACG9BE_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN3031106.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            11516..12967
                     /locus_tag="ACG9BE_000320"
     CDS             11516..12967
                     /locus_tag="ACG9BE_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFN3031107.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            12960..13682
                     /locus_tag="ACG9BE_000321"
     CDS             12960..13682
                     /locus_tag="ACG9BE_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN3031108.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            13702..14853
                     /locus_tag="ACG9BE_000322"
     CDS             13702..14853
                     /locus_tag="ACG9BE_000322"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN3031109.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(15201..15476)
                     /locus_tag="ACG9BE_000323"
     CDS             complement(15201..15476)
                     /locus_tag="ACG9BE_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFN3031110.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            15584..16348
                     /locus_tag="ACG9BE_000324"
     CDS             15584..16348
                     /locus_tag="ACG9BE_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFN3031111.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            16472..16975
                     /locus_tag="ACG9BE_000325"
     CDS             16472..16975
                     /locus_tag="ACG9BE_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN3031112.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            17240..17887
                     /locus_tag="ACG9BE_000326"
     CDS             17240..17887
                     /locus_tag="ACG9BE_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFN3031113.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     regulatory      17930..17997
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            18048..19316
                     /locus_tag="ACG9BE_000327"
     CDS             18048..19316
                     /locus_tag="ACG9BE_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFN3031114.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     gene            complement(19493..20695)
                     /locus_tag="ACG9BE_000328"
     CDS             complement(19493..20695)
                     /locus_tag="ACG9BE_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFN3031115.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            21222..21947
                     /locus_tag="ACG9BE_000329"
     CDS             21222..21947
                     /locus_tag="ACG9BE_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFN3031116.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            21937..22611
                     /locus_tag="ACG9BE_000330"
     CDS             21937..22611
                     /locus_tag="ACG9BE_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFN3031117.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(22899..23183)
                     /locus_tag="ACG9BE_000331"
     CDS             complement(22899..23183)
                     /locus_tag="ACG9BE_000331"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFN3031118.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(23477..24784)
                     /locus_tag="ACG9BE_000332"
     CDS             complement(23477..24784)
                     /locus_tag="ACG9BE_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFN3031119.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            25273..26379
                     /locus_tag="ACG9BE_000333"
     CDS             25273..26379
                     /locus_tag="ACG9BE_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031120.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
     gene            26970..27170
                     /locus_tag="ACG9BE_000334"
     CDS             26970..27170
                     /locus_tag="ACG9BE_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3031121.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            27317..30106
                     /locus_tag="ACG9BE_000335"
     CDS             27317..30106
                     /locus_tag="ACG9BE_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFN3031122.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            30153..30680
                     /locus_tag="ACG9BE_000336"
     CDS             30153..30680
                     /locus_tag="ACG9BE_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFN3031123.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            30701..31891
                     /locus_tag="ACG9BE_000337"
     CDS             30701..31891
                     /locus_tag="ACG9BE_000337"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN3031124.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            31931..33229
                     /gene="asnS"
                     /locus_tag="ACG9BE_000338"
     CDS             31931..33229
                     /gene="asnS"
                     /locus_tag="ACG9BE_000338"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFN3031125.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            34163..34837
                     /locus_tag="ACG9BE_000339"
     CDS             34163..34837
                     /locus_tag="ACG9BE_000339"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN3031126.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            34834..35175
                     /locus_tag="ACG9BE_000340"
     CDS             34834..35175
                     /locus_tag="ACG9BE_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031127.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            35205..35564
                     /locus_tag="ACG9BE_000341"
     CDS             35205..35564
                     /locus_tag="ACG9BE_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFN3031128.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            35824..35946
                     /locus_tag="ACG9BE_000342"
     CDS             35824..35946
                     /locus_tag="ACG9BE_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031129.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            36088..38169
                     /locus_tag="ACG9BE_000343"
     CDS             36088..38169
                     /locus_tag="ACG9BE_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN3031130.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            38480..39946
                     /locus_tag="ACG9BE_000344"
     CDS             38480..39946
                     /locus_tag="ACG9BE_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN3031131.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            40231..40729
                     /locus_tag="ACG9BE_000345"
                     /pseudo
     CDS             40231..40729
                     /locus_tag="ACG9BE_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            40803..41243
                     /locus_tag="ACG9BE_000346"
     CDS             40803..41243
                     /locus_tag="ACG9BE_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFN3031132.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            41256..41465
                     /gene="copZ"
                     /locus_tag="ACG9BE_000347"
     CDS             41256..41465
                     /gene="copZ"
                     /locus_tag="ACG9BE_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFN3031133.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            41465..43651
                     /locus_tag="ACG9BE_000348"
     CDS             41465..43651
                     /locus_tag="ACG9BE_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN3031134.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            43671..45842
                     /gene="copB"
                     /locus_tag="ACG9BE_000349"
     CDS             43671..45842
                     /gene="copB"
                     /locus_tag="ACG9BE_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFN3031135.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(45954..48125)
                     /locus_tag="ACG9BE_000350"
                     /pseudo
     CDS             complement(45954..48125)
                     /locus_tag="ACG9BE_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(48115..48867)
                     /locus_tag="ACG9BE_000351"
     CDS             complement(48115..48867)
                     /locus_tag="ACG9BE_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFN3031136.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            complement(48864..49187)
                     /locus_tag="ACG9BE_000352"
     CDS             complement(48864..49187)
                     /locus_tag="ACG9BE_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031137.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            complement(49201..50076)
                     /locus_tag="ACG9BE_000353"
     CDS             complement(49201..50076)
                     /locus_tag="ACG9BE_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3031138.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            50403..51692
                     /locus_tag="ACG9BE_000354"
     CDS             50403..51692
                     /locus_tag="ACG9BE_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031139.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            51766..52335
                     /locus_tag="ACG9BE_000355"
     CDS             51766..52335
                     /locus_tag="ACG9BE_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN3031140.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(52511..52687)
                     /locus_tag="ACG9BE_000356"
                     /pseudo
     CDS             complement(52511..52687)
                     /locus_tag="ACG9BE_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            52766..53464
                     /locus_tag="ACG9BE_000357"
                     /pseudo
     CDS             52766..53464
                     /locus_tag="ACG9BE_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT        17489 a         9342 c        11263 g        15387 t
ORIGIN      
        1 taaagttcac aaacatcgaa aagtatagag cccgaatttt acgaatattt tttgtaatat
       61 aaaagtatag atactaaatt cttattcgtt aaacttacgc cataaggaaa ctagaaaaag
      121 acagaattat cataaaaaat ttttgttatt tttaaaaatg tacaacaaaa tttcgactat
      181 cagaccggca tgagagaaaa gttatctaaa aaataaaaaa tcctacttta tttagagaaa
      241 gccattttta tttacatagt agtctattag gcccatttgc ttgtcgatag tagaaatagc
      301 cacagatgta taaagaatat tttttcttta cttctctaga ccatttagct gaatctttgc
      361 caagattcag cttttttttg ttttttattt gtcaactggt atttttgagg tatcttttga
      421 taccagttct aaattacctt gaaaccttgt tataatagct tttttacgcc aaaaaccctt
      481 tgacaaatga ggaatatgtg ggtattcttg tgttgtggtt ataaccagta aagaagaggt
      541 atttttatgg agagctcaag taaaggaaaa tcattatatt atcaactagt tgacgtattg
      601 aaagaacgta tcgaaacaca gatgacacct catgacaaat tgttatctga aagagaactt
      661 tgtcagcatt atggagtgag tcggacaacg gttcgccttg ctttgcaaga attagagacg
      721 caagggtata tttatcgccg acacggaaaa ggaacatatg tgtctgatct atcagataag
      781 tcgtctgatc tggcgggagc gtatagtttt acagaagaga tgagggaatt gggacgggtg
      841 cctaatacag tcatcttgga attcaagcaa atggaagcaa acaaaaaggt tgccaagaat
      901 ctaaacatca atttaggcga aacagttttc aaattgaaaa gacttcgttt agcggatgac
      961 gaaccattga tggtggaacg ttcttattta cctgcaaaac tatttattgg tcttgatctg
     1021 ccattacttg aaagtaagcc attgtatgat gtgttttctc aggattatga acagcaagtc
     1081 caattagcag atgaagaatt ttatgcaagt atcgttcaaa agaaagatgc tgatccgctt
     1141 cgtatcgctg atggtgcccc ggtattatat ctaagccgaa cgacctataa tgtgaaaaat
     1201 gaaattattg aatatacgga tagtgtagcg agagcagacc agtttcatta taagatccgc
     1261 cacgtccgaa aatgattgag agggaaaaca acatgtttga actatcgaag gataaattaa
     1321 aagagttggg tgcggaaata actacgcgag aaatcaaaca gcaaccgcaa ttatggaaag
     1381 aaacagctga gatattagca cagaatcaag aaaaattagc attgtttttt gaccagataa
     1441 aaaaagcagc aaatggcaaa agagttcgag tgatttttac tggtgcaggc acatcacaat
     1501 atgtcgggga tacagcagta ccttatttaa atgaatacgg tgataacgat cactttattt
     1561 ttcatagctt tggtacgaca gatatcgtgt cttcaccaaa agaatttcta tttccggatg
     1621 aaccgacatt actggtgtca tttgcacgta gtggaaatag tccagaaagt gtggcaacag
     1681 tcaagattgc caatgaacaa atcaagacat tgtttcattt gacgattact tgtgctaaag
     1741 aaggtcagtt agcaaagaat gcagcgacag atgaacgaaa ttatctttta ctgatgccag
     1801 aaaaatcaaa tgatgcagga tttgccatga caggaagttt tacgtgcatg ttgttgagtg
     1861 cgttatgggt attcgaccaa actgccgttg agaaaaaaga aagttatctt caacaattga
     1921 tcagaactgg agaagatcta atttcacgag atcaagaaat ccaaaaatta ttgcctaagt
     1981 catttgaacg tatcgcctat cttggttcag gaagtttggc tggtgcaacg agagaagcac
     2041 aattgaaaat attggaattg acagccggta aagtggcaac gatctttgat tcttctatgg
     2101 gcttccgcca tggaccaaaa tcgtttttaa atagcgaaac acttgttatt ggttttgtta
     2161 ataaccaacc gtatacaaga aattatgact tagatattct agaagaaata aaagatgacc
     2221 aaattaccac gagtgtcatc gcgatcgccc aaccaggaaa catcgatttc acaggtacaa
     2281 gtcttttgtt agaagcttcg atcagcctac ctgatgcgta tcttgcctta cctatgatca
     2341 tgacagccca aatcattgcg ctcgatacgt cgataaaagt agcgaataaa ccagatacac
     2401 catcgcctac aggtacagtc aatcgagtgg tcaaaggtgt cattatccat gaatatgtga
     2461 acaaagggga aacgaaggat gaatgaagaa aaaattgcag ctatgaagag tttatcaaca
     2521 aaagaaggaa tcatcagtgc attggcaatt gatcaacgcg gtgctttgaa aaagatgatc
     2581 aaagcgttag gaaaagaacc aacggcgcaa cagatcactg aattcaaaga attagtatca
     2641 gcagaattaa cgccttatgc ttctgctatt ttactagatc cagagtatgg attgccagct
     2701 gctaaagcca gacatcagga agcaggacta cttttagcct atgaaaaaac gggttatgat
     2761 gcgacaactc caggacgtct gcctgattta ttagcggatt ggtctacgct tagattaaag
     2821 gaacaaacag ccgatgcgat caagtttttg ctttattatg atgtagatga agacccagag
     2881 atcaatcatt tgaaacatgt atttatcgaa cgtttaggca gtgaatgtgc aggtgaaaag
     2941 cgtccgtttt atctagaatt ggtttcgtat gatgccaaaa ccaatgacag cacttcatta
     3001 gaatatgcga agatcaaacc gcataaagtg atcgagatga tgaaagaatt ttctgatcct
     3061 aaatataaag tggatgtatt gaaagtagaa gtgccagtca atatgaacta tgtagaaggt
     3121 tattctgatg gtgaaattgc gtatagtaaa aaagaagcag cggcattctt caaggaacaa
     3181 agtgatgcaa caccgttacc atttatcttc ttgagtgcag gggtttcgac tgcattgttc
     3241 caacaaacat tgatctttgc tcatgaatct ggttcaacat tcaacggtgt gttatgcgga
     3301 cgtgcgactt ggaaagatgg agtcaaagca tatgttgaaa atggtagaga aggtgcgatt
     3361 gagtggttga gaacacaagg gcgacaaaat atcgaatcgt tgaataaagt gataaatgaa
     3421 aacgccaccc cttggtttga caaagtaaaa gaagattcgg tgacagcaaa atgatcacga
     3481 cagttaccat gaatccttca attgacatcg cttacacgtt ggatgaatta cggatcgacc
     3541 aagtcaatcg ggtcaaaacg gtcaacaaaa cggctggagg aaaaggcttg aatgttgctc
     3601 gtgtccttca tttattagca gatgatgttt tagctactgg attagtcggc gggcagcatg
     3661 gagcatatat caaagaacaa ctcgatcaat cagggataac gcatcaattt ttacctatca
     3721 aaggggaaac gagagattcg attgctattt tacatggagt gaatcaaaca gaactattgg
     3781 aaagcggtcc tgttgtgacg gaagaagagc aacataaatt taccgagcat tttgcacgac
     3841 taatggaaac aactgatatc tttacgattt cgggtagtct gcctcaagga ttttctaaaa
     3901 gttattatgc agagttactt gccctaacga ataaaaaagg aacacgagta ttgttagata
     3961 cttctggtca aaccttgctt gaagtcttgg aaggcaagga aaaaccattt ttgatcaaac
     4021 ctaatttgga agaattagga gagttggtcc accaaacaat ttcttcggct acttcgttag
     4081 atgaaatcca atcattattg gatcatccgc tttttgaagg aatcaattgg atcgttcttt
     4141 cgctaggcgc aaaaggagca atcgctaaag tagggagaca cttctatcag gttacgattc
     4201 ctaaaatcgc ggtggtgaat cctgtggggt caggagattc gactattgca ggcttagcac
     4261 atgctctatc aaagagctat acgattgaag aaacacttgc gtttgcgatg gcctgtggga
     4321 tggcgaatgc acaacaagaa aaaacaggat ggatcgatcc tgtgaaagtc gattatttta
     4381 ggggggaaat caacgtcaga aagattcgtt gattataaaa ttagaaagcg gtttctaaaa
     4441 acgaggagga aatttgcttg actagatttg aaataaaaga agagtttttg ttagacggtc
     4501 aacctttcaa aattttatct ggtgccatcc attatttccg agtgcatccg acgaattggg
     4561 agcggtcatt gcacaatttg aaagcgcttg gatttaatac tgtggaaacc tatgtgcctt
     4621 ggaatctcca tgaaaaagaa gaaggaacct atgatttttc gaaaatattg gatctttccc
     4681 attttctaga gttagcgcaa tcccttggat tatacgcaat cgtacgtcca gccccttata
     4741 tttgtgcaga atgggaattt ggtggcttgc ctgcttggct attgacaaag gagtgccgta
     4801 ttcgttcagc ggatcctcgc ttcatgaagt atgtcaaacg gtattaccaa acgctactac
     4861 ctaaaatcgt tcctcatcta gtcactcatg gtggcaacgt cttaatggtg caggtagaaa
     4921 atgagtacgg ttcgtatggt gaagagaaag catacctgcg ccaagtaaaa aaatacttgg
     4981 aggaaggtgg aatcgatgta ccgctattca cttctgatgg accttggcaa gccacattaa
     5041 gagcaggcat tttgattgac gatgatgttt ttacgacagg gaattttggc tcaaaagcaa
     5101 aagaaaattt tgcagatatg gcccagtttt ttgaagcaca tggtaaaaaa tggccgttga
     5161 tgtgtatgga attttgggat ggctggttta atcgttggaa agagccgatc attaaaagag
     5221 atccggaaga attagcggat gcagtcaaag aagcattaca gatcggcagt atcaatctct
     5281 atatgttcca tggcggaacc aattttgggt ttatgaatgg ctgttcagcc agaggcgcga
     5341 ttgatttgcc gcaagtcact tcctatgatt acgatgcacc attggatgag cagggaaatc
     5401 caacggaaaa atattttgct ctgaaacgaa tgatggcaga aaattttcca gaaatgcagc
     5461 aaatggaacc atggataaaa gaaacaatcg tcaaaaaagg aatcaacttg aaagaaaaag
     5521 tcagcttttt cgcaacactg gataccatca gcaagccgat ccactcgacc tatcctcaaa
     5581 caatggaaga attgggtcag aatactgggt acctattgta tcgaacaacg atcaaaaaag
     5641 atgcggtcaa tgaaaaatta cggatcatcg acggacgtga tcgggtccat gtttttctcg
     5701 atgaagaaaa gcaagcgatt caataccaaa cagagatcgg tgagaccatt ccattgacac
     5761 ttgagaaaga agaccatcaa attgatctat tatttgaaaa tatgggacgg gtcaactatg
     5821 gacacaagct gctagctgat acgcaaagaa aaggaatacg tacaggagtg atgagcgatc
     5881 ttcattttat cacagaatgg acacagtatt gtttaccttt agagactaca gaacacatcg
     5941 atttttcaaa aaaatgggaa gcaggtcaac cagcatttta ccgctttgaa gcggaactgt
     6001 cagaaatcgg tgatacttat ttggacctta gtgagtttgg gaaagggatc gtttgggtga
     6061 atggcacgaa tattggtcgt ttttgggaag taggaccaat tctttcactg tttgttcccg
     6121 agggtttatt tagaaaagga caaaatgaaa tcgttatctt tgaaacagaa ggtagattca
     6181 gtgaagttat agattttgtc aaagaaccaa tagaaaaaag ttagaaaagg agaatggatg
     6241 atggcaatta ttggagtaag aattgatgga cgtttgatac atggacaagt ggcaaatctt
     6301 tggacgacaa agttgaatat tggtcggatc atggtcgtag acgatgaggt agcaacaaat
     6361 gcgatcgaaa aaagtgggtt aaagttggca acgccagctg gagtcaaatt aagtatttta
     6421 cctattgaaa aagcagccgc aaatatttta gcaggtaaat atgattctca acgagtgctg
     6481 atcgtagcga aaaaaccaga tcgtatctta gcgctagtag aaaaaggtgt accgatcaaa
     6541 gaattaaatg tcggcaatat gtcacaaaca gatgaaacac gttccatcac aaaatcaatc
     6601 aatgtcgtag atgcggatgt agaggtcttc aagaaattag atcagcttgg cgttcatttg
     6661 atcgctcaaa tggtgccaaa tgacaaagca gaagacttta tgagtctttt aatcaaatag
     6721 gaggaatgat tcatggatat tcaatggtgg cagatattat tattaacatt atatgcaggg
     6781 tatcaaattt tagatgaatt acagatttat tcttcaatga gcgccccagt ttttgcagga
     6841 ttatatgctg ggttagtaat gggtgattta aaagcaggtt taatcattgg tggaagtatg
     6901 cagttgactg ttttaggggt agggacattt ggtggtgcat caaaaatcga tgccaactct
     6961 ggaacgatct tagcaacagc tttctccatt tctctaggga tgaacccaga acaagcgatt
     7021 gcggccatcg cggttcctgt agcaagtttg atgatccaat tagatatttt ggcacgtttt
     7081 gcgaatacgt attttgcaca tcggatcgat caacatgtgg aaacaatgaa ttacaaagca
     7141 attgaacgga acttcttgat gggggcatta ccttgggcac tttccagaat ggttcctgta
     7201 ttcttggccc tagcattcgg tggtggctta gtccaaaatg ttgtggctgt tttaaacggc
     7261 gacctaaaat ggctaggaga tggcttatct gttgcagggg cagtcttacc agcagttggg
     7321 tttgcaattt tactacgcta cctaccagta aagaaacaca tttcttatct gattttagga
     7381 tttacgatca cagcgttatt agcaacagtg ttctcgaatg tccaattgtt aggaacaagt
     7441 gtggctagcg tggtcaaaga tttcccagga acattcaatt cattaccgat gcttgctgtg
     7501 gcattgattg gtctagcact ggcaacgatc agttataaga atgatcaaaa gaatgaccaa
     7561 catccagtag taagtcagca atcacaatct gatgaggggg aaatcgaaga tgacgaaatc
     7621 taattataaa ttgaccaaag aagattttaa gcagatcaat cgtagaagtt tgtttacttt
     7681 ccaattaggt tggaactatg aaagaatgca aggttcaggt tatctgtata ctatcttgcc
     7741 acaacttaga aaaatctatg gtgatgatac accagaatta aaagaaatga tgaaaacaca
     7801 tacgcaattt tttaatacgt ctaatttctt taacacgatc attactggga tcgatcttgc
     7861 tatcgaagaa aaagaaggag tctcttcaaa ggaaactgtt tctgggatga aagcaggatt
     7921 gatgggttcc ttcgctgcaa ttggtgattc tgtatttgcg gcattgatcc cgaccatatt
     7981 tggagcttta gccgcaaata tggcaacaga tggtaatcca gtaggtatcc ttatctggat
     8041 ccttgctcag gtcgctgtca tggtctttcg ttggaaacaa ctagaatttg cctacaaaga
     8101 agggatttct ctagttacca cgatgcaatc acgcttgact gcattgacag atgcggctac
     8161 tttactagga gttttcatgg ttggagcgtt agtagcaacc atgatcaatg ttaaattctc
     8221 atgggcacca acgattgggg atgtaacgtt gaatatgcaa aactatatcg atatgatctt
     8281 acctaaactt ttaccagctg gaatcgttgg tggcgtttat tggatgttag gtaagaaaaa
     8341 tatgacacct actcgagcaa tattcatcgt attgatcgta tgtgtagcat tatctgcatt
     8401 aggtgtgatt tcgaaataag ggagaattgg aaagatgagt agacaactag tcttgattag
     8461 tcatggtaaa ttttgtgaag aactaaaaaa cagtacagaa atgatcatgg gaccacaaga
     8521 aaatatctat acgatccctt tattgcctga agatggcgcg gacacgtata aagaaagatt
     8581 cctctccgtt acagcaccat tgaaagatta cgtagtgctt tgcgatctgt tgggtgggac
     8641 acctgcaaat accgtttcca agctaatcat ggaaggtgaa acgatcgaac tatatgctgg
     8701 catgaatttg ccgatggtca tcgaattcat caattcgcaa atgattggag cagaaccaga
     8761 ttttattggt tcgggtcgtg agagtatcgt ttcagtgaac caagtgattg ctggcatggg
     8821 agacgatgaa gaagaataat caagcacaac ttcttgactg gtgatacaac tggatataaa
     8881 tacggatgta taaagaggcc gtgtataaaa aagccaacgt accgcgaaat atagttttct
     8941 atatttcaca gtacgttggc ttttatcgtc ataaaatcag aaatgatctg ggtcgatcga
     9001 agaagctagt tacaaataac tatttacgct tttgctgttt accagcattc cgaccattct
     9061 tcgcattgtt ttttggtgct tgtacttgtt tttttgtttg cttcgcaggt tcagcaggtg
     9121 tgacatcttt acgaggcgtc accacttgtt ttggcggatt ttgttttaat tcttccgcaa
     9181 cttgcgcttt taatcttggt ttcatgatga cattcgtaat gaatgtttgt agacaactaa
     9241 agatcccacc gactacccag taaagagtga caccagctgg ggcactgatc gacatgaaga
     9301 cgatcatagc gggtgatacg atcaacatcg tacgcatcgt tttcttttgt tcttcaggga
     9361 taccgatagt tgagatatag ccttgaagta aataagcaat accggcaatc gctactaaaa
     9421 ctaagctagg gttacctagg tcgatcccgt aaaaggttga ttcacggatg ccttctgtat
     9481 agcgagcagt gaaatatagc gcagaaaaga tcggcatttg gatcagaagc ggtaaacaac
     9541 cgataccacc agtcatactt aagccatttt cacgataaac ttgttgcatt tcagcttgtg
     9601 cagccatttg ttcttctcga gttgtcgcag ctttcaattt ttgttgcgta gcttcaactt
     9661 gtggtttcaa tgcttgcatc ttttcagatt ggatcattgt ttttttcgat tgactaatac
     9721 ctaatggcaa aatgatgata cgaacgatga ctgtcatcaa gataacagcc cagccgtagg
     9781 accaattaaa gttttccact aaatatgtaa tcgcttgtcc cattgggtga accaatatac
     9841 ggtaaatgat tcctgaagtg tcagggttac catttgaatc catgcggaca caaccggaca
     9901 agaaaagcat gatcgtgaca agcccagagc ccaataacca atttttccat ttattcataa
     9961 aatacttcct cttttataat ttttacacaa gcttaactat actaaaaaag cgctcataat
    10021 tcaatcaact tcatagaaat ttcatagaaa taaccgattg gaggatcaaa aaagacgctt
    10081 aatgttaaaa agaagaatcc agctaaaaat acttcagtat aaagcatact tagcacctcc
    10141 gttctctaaa ataagatgag caacggatat ttccttgaaa tcataatttt agcgcaaaaa
    10201 aaagaagtgg aatgtcaatt ttttccactt cttttttttt accattgagc cgatccataa
    10261 aaaagctgac tgccatggta taaggggcta actatccctg tgtcacttgt tctaactctt
    10321 tcattttctg gacaaccagt gctggtgtga tcgtttcttt catgaagtgg atcatttcat
    10381 caggtaaaca tgcacgttca gctacggctt gatagtcact ttctgttaaa tgtaaaccca
    10441 tatcagctaa actcgttggt aagttcaagt gatgataaaa cggcagtagg tgttcgattt
    10501 ctgctaattt attttcgatc atcagctgga caaggatacc gtatgcaact ttgtttccat
    10561 gaagttgctg atggctatca gggagtacgg tcatcgcatc gtgaatggaa tgagctccag
    10621 ctgttcggcc gtagtcatcg ccaaacccac caaccatacc agccaataaa atgtttgttt
    10681 ccacgacatc gataaaagct tgattgattt tttgctggtc catcgcttcc attgcctctg
    10741 cacttttctc taaaaggatt tcgcggcatc gtaacgctgc aaattccgct acttggactt
    10801 cgataggctt gatagttaac tgagagatga ttgcgtgaga ttcataccat tttgctaagg
    10861 tgtcaccaat accggcaatc atcatctcgc gaggggagtg caaaatgatc tttggatcga
    10921 tcaacactaa atcatttgct ttagggaaca tatcatagcg aagcatcact ccttcttcat
    10981 tatagaccac actaagtgga gtataagctg cgcacgtcgc agctaaggta ggtaagatga
    11041 tgattggtaa gtttgtctta taggcaatca attttcctag atctgctact ttcccgccgc
    11101 cgactgcaat aataccattg aaatccttct ttttgcataa agaaacaaaa ctgtcagcaa
    11161 gtggatcggt acattctcca ccataatatt caaaaatttt tgttattgat aaatctggaa
    11221 aaacatcttt tactgctttc caagatgttt ttccatggag aatcaaaacg ttgtcaatat
    11281 gccttttgta aaggtgttca gggagtgaat cccatgcgtt ttcttttatt tcatactctt
    11341 gaggggcacc gcgaacaatt aaattttttg tcatataaaa aacttcttcc attatttttt
    11401 taaattaatc tttttttatt ataatagtta tgctagtata ttagcaagtt atttatgaat
    11461 atttattctt ttatagcttt tctgattaca ttattattca aaggtggcca aatggatgaa
    11521 aaaaggaata aacatattat tagtggcgct gatgtttcta ctggtttttt taccagtaaa
    11581 agtacaaggt gcagaaaaag atgaaattta cgagcggata aaacaaagtg gagaattagt
    11641 cgttggccta tctgcagact atgcgcccta tgaattccat gcgaatgtgg atggtaagga
    11701 taaaatcgtt ggttttgatg tatccattgc cgaaaagatc gcaaaggata tgggtgtgaa
    11761 actaaaagta gaagaacttg gttttgatgc attattaggt gcgttgaaaa ctggcaagat
    11821 cgatttagtt atatctggta tggcggtgac tgaagaacgt ttacaagaag ttgacttttc
    11881 tgacccatat ttcgtggtac agcaaaaagt actcgttcgt aaagaggaca aaaataaatt
    11941 taagacgaca gccgactttt ctggcttagc cgttggggcg caaaagcaaa ccacacaaga
    12001 ggagttagtg aaaaatgaac tgactggtgc agagttgacc tccctgcaaa aagttcctga
    12061 tctgatcaat aatttgctga atgaaaaagt tgaaggtgtt gtgctggaac aaccagttgc
    12121 agaagcatat gcccaacaga ataagaaagt agctttgact ccagtaaaat ttgcgcaagg
    12181 cgaaaaaacc acagctattg cgatgtcaaa aaatacacca gaactgaaaa atcatgtcaa
    12241 tgcgtcaatc aaaacgatca atgacgacgg cttgctgaaa gattatcaaa aacaagcgaa
    12301 tcaattgatg ttccaagatg atcagtcctt tcttcaaaaa tacggtccgt attatttgat
    12361 tggaacaaaa tacactattt tattagcttt tgtgggtgta ttgtttggaa tggtctttgg
    12421 tagtttgctt gcactgatga aattgggtaa gaataaattt ttaaaggcat tagccattat
    12481 ttatatcgaa tacgtacggg gaacacctct cttagttcaa atctttatcg tttacttcgg
    12541 aacaggagta ctgggattgg atctatcgaa actggtagca ggttgtattg ctgtctcgtt
    12601 gaatagtgct gcttatgtag ccgaaattgt ccgtgccggg atcaatgcgg tcaacaaagg
    12661 acagatggaa gctgcacgtt ctttaggaat ggaccagaaa gaagccatgc gtttcatcat
    12721 cttcccacaa gcaatcaaaa atattcttcc agctctagga aatgagtttg tcacgatcat
    12781 caaagagtcc tcagttgttt cggtcattgg ggtatcggaa ttgatctttc aaacgggcgt
    12841 ggtacaaggt gctagcttca aaccattcct accatatgtg ttggtctctt tgatttattt
    12901 cgtgttgacc ttcagtattt cccgtatatt agctttagtt gaaaggagaa tgggtctgaa
    12961 tgcttaatgt aaaaaaactg tcaaaaagtt ttggcgagaa tgaagtctta aaatcaattg
    13021 atctttctgt gaaagaagga gaagtagtgg tgatcattgg cccttcagga agtgggaaaa
    13081 gtacaattct acgttgtatg aatctgctgg aagaaccaac ttctggtgaa atctttttta
    13141 agggacaaaa cattacagcc cctgacagcg atatcaatca aattcgtcaa aaaatgggaa
    13201 tggtatttca aagttttaat ttgtttcccc atatgaatgt actcgaaaat ttgaccatca
    13261 cacctgtaaa aatcaaaaaa gaagacccta aaaaagcaaa agaacatgct ctatcattat
    13321 tgaaccaagt tggtctaaag gaaaaagtgg acagttatcc taccagttta tctgggggac
    13381 agcaacaacg ggtagcaatt gctcgagcat tagcaatgaa tcctgatgtg atgcttttcg
    13441 atgaacctac atctgcactt gatcctgaaa tggtcggtga agtgttggca gtcatgcagg
    13501 atttagctaa aaaagggatg acgatggtcg tggtcactca tgaaatgggt tttgctaaag
    13561 aagttgccga tcgtgtgatt tttatggctg acggcatcat tcaagaagag ggaacaccga
    13621 aagagatttt tgaaaatccg caaaatagtc gaacacagga tttcttgaac aaagtgttgt
    13681 aacaaagatg gaggaagtaa gatggacaaa agtaagattg cacgccggtt tcagcaacca
    13741 gcagaaaatc tgttgatgaa tattgctatt ttggcaaagg aaaaagagga tattatcgat
    13801 ttatcgattg gtgatcctga cttgatcact gatccgacga ttattgaagc agcttataca
    13861 gatgtaaaaa atggtgctac taattatact gcgcctgatg gcagcaaaga attcttgtca
    13921 acggtcgtag atttttatca aaagaaatat ggattgagcg ttgaatgcaa ccaagttcga
    13981 gctacagtcg gagcaatgca cgggatgtat ctagttttac aggcattact agatccagag
    14041 gatgaggtca tcatccatga accttatttt tcaccatatc gggaacaagt cattttatct
    14101 ggaggcaagc ctgtttttgt tcctacctat gaagaaaagg gcttccagtt ggatgtggaa
    14161 gatttaaaag cagcgattac ggatcgtaca aaggtgatcg tcatcaattc gccgaataat
    14221 cctacgggag ctgttttttc tgaaagcacg ttgaaaaaaa tcgctgaagt cgcaatcgaa
    14281 aatgaactct atatcatttc cgatgagatt tatgaagact ttgcctttaa agagaagttc
    14341 gtaccgatgg cgcgatttgc gcccgataat acgatcactg tcagtggtgt atcgaagggg
    14401 tttgcgatga ctggctggcg cttagggtac atgatcgcac cagcatacat caatcaaatt
    14461 gctgggttga tcaatgaaaa catcacttat tccgcaccga ctgcttctca aagaggtgca
    14521 atctacgcct taaagcacta tgaacagctt gtaccgccag ttgttgaagt attcaaagaa
    14581 cgattagctt atattgagaa aagagtggca gaaatccctt acttatcttt atcttctgtt
    14641 tctggaagca tgtatgcttt tattaatatc agtaagagtg ggctcgattc agtaacgttt
    14701 gtggaagagg ttttagaaaa aagcggcgta ctttttgtcc ctggaaaagc gtttggggaa
    14761 acgatcgggg acaactatat tcgactagca gcgactcagc cattggaaat cttaggtgaa
    14821 gcctttgatc gtttagcaaa actaaatttt tagataaaac tgtatatttg aaaacagcta
    14881 tgggtatcca ttagaacaaa gagggaaaat gatcctttcg agagttggct tgttgactgg
    14941 gcagcgtcag gatttgacaa tgaaaaagtg gtagtggaaa atcaaagtcg attttccact
    15001 accataaaat attatagact ccattttatt gaaccaaaat ttttgaagtc ctcccgcttt
    15061 tacgtatgcc tatcactggt tactaaactc gctaataaga atgttttttt gtcgatctag
    15121 gatggagaga ggggcaaact ttttcgatct acatacagac agtccgattt ttctgacatt
    15181 tcctattcat ttctagaagg tcagcctatt acaacaaact tttttctgtc ctccatcatc
    15241 ggattttcct gaaggtctac atattggaca cgtccgctgg ggcttggtga tcgtttgact
    15301 tcttccacga attttttgat tgcctcatcg tcgcccgcag cttcgatcgt gacagagccg
    15361 tcatcttcat ttttcactgt tccggtgatc cctaattgat ccgctacgat ttttgtcata
    15421 tagcgaaaac caacgctttg tacacgtcct tgcacgttca tcttgatttt gttcatttta
    15481 ttcaccgttc tttcttttat ttttaaatta atgataccga cttttttgct actgaacaag
    15541 tgcaatgatc taatccatgg tataatcagt tgaggtgaaa aatgtgaaag agatccaatc
    15601 agcaaaaaat ccaagaatca aagaattaaa aaaattacat aagaaaaaat atcgtgaaga
    15661 aaaaggggaa tatctgcttg aagggtttca cctgatcgag gaggctgttg cctcaaatgc
    15721 gaaggtcaac gaacttttta ttacgcaacg cggtctgact gagtgggcta gttggcttga
    15781 agaaaatcag gcgctttctg ttacactagt atcagaagaa gtgatggcaa ctgtttcaga
    15841 tctaccaact ccgcaaggga tcatggcagt ggtggaagct gaaaaaagag agataccgaa
    15901 ttttgaaggt ggttggttgt tgctcgacaa cgtacaagat cccggcaatg tagggacaat
    15961 gatccgcaca gccgatgctt ttggtctatc ttgtgttgta ctaggtaagg gaacggcgga
    16021 tgtctacagt accaaagtat taagaagcat gcaggggagt aactatcatt taccgatcat
    16081 ccaagaggat ctaacaaaac tattgcctat ccttaaagag caaggattta taacctatgg
    16141 aacagaactg aatgaagaag cagtgtccct gaatgatatc cagacaacag aaaattatgc
    16201 gatcgtgatg gggaatgaag gtcaaggcgt acgtccagaa gtgctggcgc taacggataa
    16261 aaatgtgtat ataccgattc aaggaaatgc ggagtcttta aatgtaggag ttgcagcggg
    16321 catcatgctt tatcacttct ccgtatagtt aaggaactat taagctttct gcattttgcg
    16381 aaaacctcac tagaatatta cgaacactag tggtatacta agttcaattt caaagataaa
    16441 tggaattcaa aaaggtggca gatcgataat tatgacagaa aaatggcgtg aagacgaaga
    16501 atatatgtct tatataaatg atctattaga aactgatgca gtaaagaaat tagcaaatta
    16561 cacgcaacat gtccactcga cacgattaga gcattcaatc agtgtgtctt actatagcta
    16621 caaattagcg aaaaaatggg gcggtgatgc aagagcgact gctcgagcag gccttttaca
    16681 tgatttattt tactacgatt ggcgtacgac gaagttcgat gaaggtacac atgcgtatgt
    16741 tcacccaagg atcgctgtga aaaatgctga aaaaatcacg gagctttcag atctagagcg
    16801 tgatattatt ttgaaacata tgtggggagc aacgattgcc cctcctaaat ataaagaagg
    16861 ttatatcgtt acttttgtgg ataaatattg tgcagtcaaa gaagcagctc aaccaatgac
    16921 agcttcaatg aaacaaaaat ggcaacgata tttcggtaaa gaacaatcga tttaaggtag
    16981 ttaactaagt atttgacccg agtattttga aaaaaatcct cgggtttttt gtttagacat
    17041 tgactaattg gtctttttag tgtttacttt gttgagatga aaaagtacat aaccaacata
    17101 aaaataaagg gggtgatgat aagatgcgat gtgtatctta tttaaaagag tttgagggct
    17161 ttatctaaag gcactttgat agaaagatca cttttttctt caaagtagcc gattttgaaa
    17221 gaagaaagga ctcaaagtca tggaaaaaac aatcaaacca tatcaatatt attttatcaa
    17281 gcgagaagtc gagcagctgt taagcgccta tagttcagtg aatgatccaa agacagtcca
    17341 aacagtccaa gcattagctg cggaaaagat ccacgaggta cttggtcatc ctaatacagc
    17401 tgtaaatgaa tttatggaaa aggtactaga cgtcactcta aaaaaagaaa aagcagaacg
    17461 actattagag gaattgaaag tgatcgttct tccattcata caaccgacaa aaccgcagat
    17521 cgataaatta ttccgaaaag tgaaaaagtt aaaacaacca gattggaatc aactggattt
    17581 aagggaacat acttatattg gttggaatga tccaggtagc caaaaaaaat ttatgatcta
    17641 ttatgaagaa gaaaaactaa aagggatcag tggaacatta tctcctacaa tcaacaaagg
    17701 aatttgttcg atttgtcaaa gaacttcaaa tgtttcactc tttctttcta ccacaaaaac
    17761 tggttcagat ggaacataca cgaaaaaagg aaattatatt tgtcatgata gtgaccaatg
    17821 taatcagcag ttggtacaat tagctccttt ttacgaattc acagaacgag tgaaacgtga
    17881 gcggtgattt cattgtattt ttctgaaaaa tatgctatct tatttggtga agtgaatagg
    17941 tgatggtgtt cgcctttaaa cgttgatcgt aagataacta atgacgccta ctaaagaaac
    18001 ggggctaccc ctttttttag taggcgtttt tttgaggagg aaatgagatg gaaaatcgat
    18061 tacctgatcc attgaaggtg atcatctata tgatcaaatg gttattgatc acagcaattt
    18121 taggtatttt tatgggaagt ttatccgcat tctttctaac tagtttgact gatgtgacag
    18181 ctattcggct ggagcatcct tggttacttt ttcttttgcc gataagtggg ggactttttg
    18241 cttatctata tacgacgtat gggggcaatt cttcccgggg aaacaattta gtgatcgatc
    18301 aagggaatgg aggaaatgaa aagattccat tacggctgat cccactgacc ttatttggca
    18361 ccattaccac gcatcttttt ggtggttctg ttggtcggga gggtacagcg gtccaaatgg
    18421 gcggggcact tgctgagaat tttgctcgtt ggctaaaatt gaacaaaatc gagcgtgaga
    18481 ttcttgtcat cagtgggatc agcgcaggtt ttagctcagt atttgggaca cccttggctg
    18541 ggacattgtt tggattagaa gtattggcaa tcggaaaagt gagaaccgaa gcactgtttc
    18601 caagcttttt tgcagcactt tttgcgaact ttgtaacgga aagtttcaat gtcaagcata
    18661 cccattatca aatgggggcg attcctgcat gggacccttc acttttcttc aaattgttgc
    18721 ttgcgggcat tgcatttggt atcattggct ggctctttag ccgttcgatc gtctggttaa
    18781 aagcttggta tgcaaagcgg atcccccatc cagttttacg caactttatt ggaggagcag
    18841 tcgtggtact cgtagtagtg attttacaga cgcaaagata tctcggatta agcttacctt
    18901 tattacaaga tgcatttaat ggaagtgctc atccttatga ttttcttggg aaacttggct
    18961 ttaccgttct atcacttggc gcgggatacc aaggaggcga agtgacccct ttgtttgaga
    19021 ttggggcaac tttaggtgcg acattagcac ccattcttca tgtatcagta ccttttttag
    19081 caggattagg gtttattggg gtgttctctg gagcaacgaa tacaccgatt gcttgctttg
    19141 ttatggggat cgaacttttt ggaggtcaag cagccagcta cttctttatg atctgtttga
    19201 ttagctttat gtgttcagga aatagtggaa tctattctgc gcaaaaagtt gaagtgaaaa
    19261 aaggaatctt gttcctaccg atccttggaa gaatctcacg acaaaaagag aaataaaaag
    19321 aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa aattgctttt
    19381 taaatttttg tgaatttcgc ttatttccga aggagttgct tctgctccca ccgtttattc
    19441 ggagttagag tctgtggcaa aactgatttt tagttttgtc ccacgctctt ttttattgag
    19501 caaatttgag aataagagca gaaaatactg caaagccaat cattaagcca cagatgataa
    19561 gatggcgttt tttgtgtaag acaaggatgg caataaattc tcttaagata gcattcggta
    19621 agtagtatag tgcagttttt gcaccgattc cgtttgcatt gagtttcgct tgtcgcgcat
    19681 atagtccagc tctaaagata tggtaattat ttgaagtaaa aatcgcgcga taaggcccat
    19741 tcatttgctg atccattatt tcttttgaaa acagcatatt ttctagcgtc gttgtggaat
    19801 tcgtttctac taaaatatca cgctcgggaa tccctttttc taaagcatat tgtttcattg
    19861 caatggcttc agctactttt tcatcggttc cttttcctcc tgacatgagc aagactggcg
    19921 gattcaaagt tgctttactt tgtgcccaat aaaatgcgat cgccttattg atccttttag
    19981 caagtagggg agatacgcgt tcaccatcaa tcaagccagc gccgagaaca acgatataat
    20041 cttggttata ccttggacgg ttgaactgat acaaaattga aacagtcata aaattaaaga
    20101 aaacgatgag tagataagca aaaataaacg gaatgacagc aaatagagca tttgcccatt
    20161 gtgggagcca atggactaat gtatagtcca atatcaaaaa gatagtcaaa gcgactccta
    20221 acaagagagt caaaaggttg gccaaagaac gggattcttt tttcatgact acaacaccat
    20281 tccaaaataa aaaaatgatc aaaatgtaga taccgattgc caaaagaatt aatattggta
    20341 tcccgatcaa agccaataac ccaccgataa agatattttg cgtctctatt agtagccacc
    20401 ctagataacc accaaagaca atcaaaaacg tattaaaaag tacgccattg attaacctac
    20461 gtttttcctt aaaatagaaa aatgcaaaaa tactgaaaaa tagcaatgga atcaaaaaat
    20521 aaaatgtatg ataaaaaact tctaaaacga cgaagtaagt gaccatcatc ataaaagtcc
    20581 acgtcccaca ataaaagaat agagcgggat cgctttggcg cttgcgccaa tagaggatgc
    20641 ctgacattcc taaaatatag ataactaaaa atatcaggtc tctagtaaga ttcattgaat
    20701 gaactccttt cgatttgtaa attttttctt aaaaaagata gcatcattta gtagtaaata
    20761 tatcgaatat agaaaagtta gttactaaat agtcaatcag ttttttcacg acgtttctat
    20821 tataacgaat tatgttgaaa aaagctttta tcgaaaacat aaatcttcaa attaacagat
    20881 tttttagacc tcgatcatga tgaagacttt tgtagataca aataactgta acattgaaaa
    20941 tgaatgcgaa aacgatattt ttttttattg gaaaatatcg gtcaagcaat aaaccagttg
    21001 cataataaaa tgttaacggc aacaaaatct gtggaaggaa tgaaccggat gatttgatgg
    21061 aaaaaacaag atagacaaga agtatacata gtaaaacgtg gaacaagtag gccagaatga
    21121 aaaatcagtt tttatccact cgcttttcta tacattcact gtcacaaata taatcgaaag
    21181 taattctatt ttgagataaa gtattttata ggagatagta tatgaacaaa aaaagattta
    21241 aacgtattga aagaaaattt gcattaactt atgaccaagc taagtatttg aaaacactat
    21301 ttccaaaaca ggtagttcgg gataaatata gtgatcattt tatttcttcc ttgtattttg
    21361 atacaaacga tcatcacttt ttaaaagaaa tcactacaga aaagtcctat cgagaaaaat
    21421 ttagaatcag aaaatatgga gatcctcagg acatatcgcc tatctactta gagataaaaa
    21481 gaaagtttca aggaatgaca gataaacgta gaataaaatt agcgcatcat gagctcgatg
    21541 atatgttgga aaatatcgaa ttggcagaaa ccacattcag taatttagaa gaagagtata
    21601 tttttaatga aatacagtgg ttgttcagaa ggttggcatt ggagcccaaa gtatatatct
    21661 attatttaag agaagcattt gtaaatagag attcagaaga tttgcgaatc acttttgatc
    21721 aaaatatcca ttaccgtcta atgaacttaa cgacctggga acactgtaca gaagacaatc
    21781 tggtagcgcc agaaatcgat gtgctgatgg aagtaaaaac gacaggagaa atacctgaat
    21841 ggttacaggt aggattggat aggatgggta aaaaagagat aaaattttct aaatataaac
    21901 aagtctataa aagatatatc caagaggacc cgctctatgt tcaatagtgt cttaaaaaat
    21961 gagccttctc aactcacact agatgatttc tttttatgta tggtggtgtc gattttgcta
    22021 ggcattctga ttgcttacac ccatatgtat caaaacaact gtacaaaaaa cttcatgttt
    22081 gccttgattg ggttacctac tttagtacat accgtcattt tgttgatcaa cggtaactta
    22141 ggcacgggga tcgctgttct aggcgcattt aacttagtgc gatttcggtc tgctcaaggg
    22201 aattcaagag atattttcag tattttttgg gcaacgtcga ttggtttagc cacagggacg
    22261 ggatatattg tctatgcact attcgtgtct agtatagttg ctgctatcca agtcatcatc
    22321 ttcacgcttc caatcaaaaa taaacataca gtttttaata ctgaaaaaac gatcaagcta
    22381 aaagtttcta aatccttgtt tctttcgagt attattgaag aaaaaattgc agaatataca
    22441 gaaaactgca atttaaaaaa agtaaaaatc aataaagaaa cttgttcctt acactatata
    22501 gtaaaagtaa aaacgagtat gcttgaaaaa gaattggttc gaacgatcat ggatcatcca
    22561 gaagtcattt cgatcactgt aggtatggat aaatcgaata aattattgtg ataaccaaga
    22621 aagtacttaa caatgaagta agaccccagt tccaaagttt agcggggaca cttcaataaa
    22681 gtactttctt ttttgtcttc agcaactact ggtaaaactg agagcaagct ttcgtgagag
    22741 tacagcgata aagatcaacc tcccttttta tctcgagcaa tttccttgca aggctgtttc
    22801 gttgatctta aacgagttag caacagtcaa agatgcgatg gccataaaaa aattgcatta
    22861 agttggctgg tatctgatag ctgggaatct aaggaagact atccattctg tttcttaaat
    22921 tccgctagtt gatagatatc agccaattgt tccagatgat caacatcaaa gataaccgaa
    22981 agatgtttcc ttttattccc atttgcttga agagttttta tcttctcttt ggttccttca
    23041 ctcaatgaga gtgtgactaa tttctccctc gttttttctg ggaaatgaaa ggatactttg
    23101 aaaaatccgt cccaaataga aagccagaag atcggtttta ctttctgaga gtgggacaaa
    23161 agtaagaaac acttttgtct cattctttaa aaacgaataa acggtgggag cagaagcaac
    23221 tccttcggaa ataagccaaa attcacaaaa atttgaaaag caattttcgt gaatttcttc
    23281 ttatttctcg gagttaaaca cttctgtccc aacctcttgt attcaccttt tgatagccaa
    23341 gcttttccat cattataaaa ccgcaaattt caaaaaaaaa cgaacaatac aaaaagcgta
    23401 ttgctcgttt ttctcttcag tttctcacac ctaaacgact tttgtctcga cctctttttg
    23461 gtgactattc atgatgttaa aataaattac tgaaaaaatc tgtgacatgg cgccaaccaa
    23521 tgacaaaaat attagctttt tcaacagtag aagtggtaat gatgtctgtc gaaggctctt
    23581 ggtttgcttc caaatagcct aatttgtcat cagctaattg gatcgttgct gagccaactg
    23641 tagtgccttt gttgatagga gcggtcactt ggtcatccgt tactttcttt ttatcaattg
    23701 ttggagtaat cgttaatttt ccagtatcca ttccattttc gacccagact tttacagggc
    23761 tttttagcgc aacattgacg gaggtttctt ttccatcttt cactttgatg tcttttaggc
    23821 ttgggatgct tgcatttgct tttccaagtt ctttttctga ccagttgtca taacaataat
    23881 ccatcaatcg tgatgtttca ttgaatcggg cacttggatt ttcttcatgg ttatctgcat
    23941 ttaaaacgac tgtgatgatt cgtcggccat ctttgttgat cgttccgaca aagcatgcac
    24001 ccgcaaaatc agttgtgcca gtttttaatc catcgacacc ttcgtgatag tttacaaaac
    24061 caggtagcat ccagttccaa ttgaccattt caaccggtga ctgtgtgttt tcgccgaatg
    24121 tttgatttgt ggtactcgtg atttttaaga cttcaggata atctaagatg aggtgacgag
    24181 caacaatagc gacatctttc gcagacagtt gattttcatc tgtttcacca gtattctcag
    24241 gtcgattctc acctaggtaa gaattgttta aacctgatgc attgacgatc gttgcatctt
    24301 tgatacccca atcttttagt tgtttcttca ttagctcaag aaatttactt tcactgcctg
    24361 caactttttc agctaaagca accatggaag cattggctga ttggatgaaa gctgagtcaa
    24421 caagttcttt cactgtataa tcattttctt tatgcaaagg aacattcgat agatccggca
    24481 cgacacttaa tttttctgca taatccgaaa tggctacctt atcttcccaa gataatttac
    24541 cctctttgat ttgttcaagt acgatatata gaccaatgat ctttgtgatg gaagcaattc
    24601 ccatcgtttt ttcgccatcc tgatcataaa ggatttttcc actttctgca tctacggcaa
    24661 aggcagcttt ggcatttatt ttaaatgcgt cgtctgcttg aacagttgcg ctaggcaaga
    24721 aagaagtgat acataagata agacttgcta atagtacaat aaaacgggta ttttttttga
    24781 acatctttgt tctcctcatt tttaaattga ttcttacaac ttagaatatt atcagagaga
    24841 acaaaaggtt tcaatggttc tgaaaaaaac cttataaaga acaagtctta ttaatgaaga
    24901 atcatagaaa gttatctttt gggttcattg acacgaactt tgactggctg aacttcgggt
    24961 tcggttaggc gtgatgctgc aaaaagggcg cctgctgaga gtgcgaccaa actaaaaatc
    25021 atgaataatt gcataagatc acctcgttct tttttgtttt tttatagtat agcaagagag
    25081 tttgaagcta ctgttaagga aatgagcata taattgatga aagtatgaaa aaaatatgaa
    25141 gtaggacaac acaactaaaa atcgatcagg tatttctctg gtagggattc gttttattgt
    25201 attgataaaa tgggatgtgg tagactttat ttacagatac aaagaaatga tttttttagg
    25261 aggggtacgt ttatggcaaa aaaagaaaaa gaattattat cacaattgac ttcagaaggt
    25321 accaaaggac cgtttgtcac gattatgtta aacacccatg ttgcgcatca agacgtagaa
    25381 aaagatcaaa tcaaattcaa gaattttgca aaggaagcga aaaagcgctt tgaaaagaaa
    25441 tactctgagc atgattggtc tccattccaa gaaaaaatta ctagtttgtt ggacgatccg
    25501 tctttctggc gtagtgccac aacgagtgta gctgtcatag tatcaccaga acaagtgttt
    25561 atccaccgtt tgggtattcc tgtagatgac cagtattatg tgagtgatct accttattta
    25621 ttagcgatca tcaaaaatgg acatttcaac tattcatact atttattagg attgaatcgt
    25681 gattcgatga aattgtacga agtaacgaat aaacaagtca aagaagtggc tttgcctgaa
    25741 gatgcaccaa gagatgtcgt cactgcttta ggatctgaac taacaggagg cgggttgaac
    25801 tacgcatcaa atggtagtga aggtacgacg ttccatggag tcaatcctaa agatgaagaa
    25861 gtcgaaattg attgggtcaa ttattatcaa gcagtcgatg actatttgaa agatacattt
    25921 gaaaatccag agaaatggcc tatcttctta tttgcattac cagaaaatca gacgatgttc
    25981 aaaaaagtgg cgaagaaccc ttattatcgt tccgatgttg cgatctcagc ttcacctgca
    26041 caactttcat taaaagaaat tgaagcaggc gcaaaaaaac tatcgcaaga attagctgtc
    26101 aaagaagcaa gtgactacaa taagttgctt gataaaaagt tcattgatca actcgttgat
    26161 attataccgg cagcaaaaga agggaaaata tcccatttat tcattgctac atctaattta
    26221 gcagatggtt ttggcgaaga tccagaaaca gaatatgacc gccgtcaggt gctgaatgaa
    26281 ctagcagata atgtgatcac gaacggtggt caggtcttta tccttgaaca aaaagatgca
    26341 ccagacgaaa agagtttagt tgcgatctta cgttattgag tgaaaactac caaagtagat
    26401 gaatggtcag gcgttgatag aagtgtatag ctccgagaga aggagtttcc tctgtttcag
    26461 ccgttggatt catcttgagc gagagacaaa agctagcttt tgtctctcgc tcattatttt
    26521 tgtgctttag tcccattggc tttgcgaggg gaagatactt ttctcaaaaa aacgaacctt
    26581 gcagttgaga gggggataga tgcgcatact aatttttatc agttaaaaaa tgatgtggat
    26641 cattagagaa ttccaaagtt cgttcatcct ttttcagata ataaattgaa attttttgca
    26701 aaagtgaaaa tagcaaatag aaattttctg aatcttatag gggtgatttc gagaaaacgt
    26761 tcgtttattt gcaaatatat cttctaaata agagaataaa tacaaaaaaa taaggaaaaa
    26821 ctctggcaat tttgtaaacg gtatgatata gtaagaatgt agtttttgtt cggtaatctg
    26881 tggtagcttg tttcaaataa acatcttcca gatatacacc attcaagtaa ttgcaaaata
    26941 tgtgttgata cacaaggagg atttttatta tggaaacagg tacagtaaaa tggtttaact
    27001 cagaaaaagg atttggattc atcactgcag aaaacggaaa cgatgtattc gttcatttct
    27061 cagctatcca aggcgacgga ttcaaaactt tagaagaagg ccaagcagta actttcgacg
    27121 ttgaagaagg tcaacgcgga cctcaagcaa caaacgttac taaagcttaa tttacctata
    27181 ttaaaaataa aagtccagac atttatgtgt ctgggctttt ttgtcttttt ttccttaaaa
    27241 agcgatgaga gtatttttct gcactttgta gtataatttc aaaagaacat tagaggcagg
    27301 agaagaggaa gatattttga aaccaagaac ttatgcagtc gttgatatcg agacgactgg
    27361 gacgaatcca aaagaagatc ggatcatcca atttggctgc gtcatgattg aagctggtcg
    27421 gatcacttca cgtttttcga tcgatattca tccagggcgg aagatttcaa aacaaatcca
    27481 gcatttaaca gggatcacga atcaacgcgt ccaaaaagcg ccttattttg aagatgtcgc
    27541 ccaaacgatt tacaatcttt tggcagacac catatttgtc gcgcataata tttattttga
    27601 ttataatttt ttgaaccatg aactcatgcg atgcggactt cctagtttga agataccggg
    27661 catcgatacc gtagaacttg cacaaatttt cttgccgacg gaaccaagtt ttcgcttagc
    27721 tgatttgtca gaaagccttg gctttattca tgagaatcct catcaggcgg atagtgatgc
    27781 cgaagtgaca ggtcagcttc ttttactgat cgaagaacga atgaggaaat tgccgatcat
    27841 cacattagag cagattgcac gattaagtag ccatacagga atggatacga gtcgtttcat
    27901 ttaccacgtg cttgaagaaa tgaaagaaaa cccggagccc cttgatctct cattagaaat
    27961 catcgatggg ctagctttgc agaaaaaaga agtggagcta ttcacatccg tccattatgg
    28021 cgagcggaca tatccaagaa aaaaacaagc aaaagaaaaa atgtttggta aaacactgat
    28081 gtatcgaaaa gaacagaaca ggctgatgaa tgctgtctac gaccatttca caaaagatga
    28141 atcaaaagat ttgatgatcg aagcagctac aggaatgggc aaaacgattg gttatctgtt
    28201 gcctttgagt tatttggcta cacctgaaaa accagctgtc atcagcaccg tttcgcttgt
    28261 tttgcagcag cagatcatgg aaaaagacat tccattactg aatcaattgc tagatcagcc
    28321 gatacaacct gtgattatca aaagctatcg ccactatatt gatttacagc gttttaaagg
    28381 tacacttgtg gaaccgccgg gacaaaaaca atatgctttg taccaaatgg ctgtcttagt
    28441 ttggctaaca caaacgaaga ccggtgattt ggatgaatta catttaacca atctgaacca
    28501 tgcattcttt gcagatgtcg cgcacagagg cactgggttt ttagcaagga atcagtcatt
    28561 ttacaaacaa gactttgtgc gtttcttgca aaagaaaatc aaacaaagca attttttgat
    28621 agtcaaccat gcatttttag tacaagaaac acagcgaaaa gaaccattgc ttccagctag
    28681 tccttatttg ttgattgatg aagtgcatca tttgccggaa attgctgaaa aagtaaatga
    28741 caaacgacta agtattaact acttccataa acaaattcag caactggaag agccgaatca
    28801 gttatttgac cgcatacaaa gttatttgcc gaaggaacat gaagccttgc gctggttgtc
    28861 gatttatcga gaagaactct ctgcactatg cgaagcgcag atttgcgtgg cagagggatt
    28921 aatgaactta gtaagcagca agcaacctaa cggacaatat ccagaagaag tcatgatgac
    28981 aaaagaaatg ttggatcagc tgccactgga atctggaaca gcaatggatc atctgcaact
    29041 tttttatcaa gagatccaac aactacaatc acagatgcgc ttgacttttg agattatcca
    29101 agaaagttgg accaaccaga tgcggatcga tttagcaagt ttatatagct tatttgatca
    29161 attagacaag caatacttgc tgatggacca atggctagaa gactggcgag atcatttgat
    29221 ccattggttc gttcccggga atacagcaca gcagatgatt tttcatctcc ttgattttca
    29281 agcagctatg ttgccgtcaa ctgtgtggta tcctcgatat gaccatattc tttatattgg
    29341 aggaacactg aagatcggac ctaatcgaca gtatctctct aaacgacttg gcattgaagc
    29401 agcacctctt aaagtgattg cttctcctta tgattatgag aaacaggcaa gaatctatgt
    29461 gccgaaagaa gcacctgcag tccatcaagt cagcaatcaa gcatatatcg attatttaga
    29521 agcagcactc acaggattga ttgaacaaga agatcggccg atccttgttt tattcacttc
    29581 tcatgatgtt ttgcaaaagg tatatcagaa aatgcatttg tctatgctag aaaaaggacg
    29641 tgaaattctt gctcaaggat ttggcgggag tcgggaaaaa cttttgaaac gcttcatttt
    29701 gtcagaaaaa agcgtcttat ttggtgctga cagcttttgg gaaggcgtag atttacctgg
    29761 agatgctttg cagattgttg tagtcacacg tttacccttc gacaacccgt tacgtccgat
    29821 ggtcaaagct aggaatgctt atctggaatc agaggggatc aatccttttt atcaggaatc
    29881 cgtgcctaaa gcagctttga agttacgcca agcattaggt cgtttgataa ggacggagac
    29941 agatcgtggc gtattactta ttttggatcg gagattagtc actgcaaaat atggtgcacg
    30001 aatcatccat gcattaccga aaaaagtacc gataaaagag ctagctatag atgaaatcta
    30061 tcaggatatc catgaattct taaagaaaaa tgaagaagga agttaaaggt gccgtctttt
    30121 ttcatcatta aaatctgcta taatgaaaag gaatgaagat aaaggagcga cgacccttga
    30181 agaaaaaaca gacaaaggaa aaaacgatta atcgcgtgtt gcttggcttg atcgtatttt
    30241 tattagcggt catcgtgatc agctcgattt tttacttgcg ttccagtcgt ccggcggtcc
    30301 aggcgaaaaa agaagcagta gaaatcgcaa agaaatatgc aaatctcgag gaagccgatc
    30361 atttttactg gtttactcga aagaaaacgt acttttcatt gacgggtacg aatgataaag
    30421 gaaaagaaat cgccgttatc attcctaaat ctggtgaaaa agtaaaagta ttggatcaaa
    30481 caaagggact gactgaaaac gaagcaaaac agacaattgc tgcggcccat cctgaaatcc
    30541 aaatcgaaaa agctgcttta gggatgtatg atgatcagcc tgtgtgggaa gtagtaggaa
    30601 aagaaacaaa tgggaaactg aattattacc tgattggctt tgaatctgga gatgaagtca
    30661 agacaatcaa gggaatttaa aaggtaaagg aatgaatggg atggagttat caaaaagaac
    30721 aaaacgatta gaaccgtctg tcactttggc agctgcggca aaggccaagg cattaaaagc
    30781 aaaaggcaga gatgtgctga gtttgactgt gggagaaccg gattttgcga caccagaaaa
    30841 catccaagaa gcagcaatcg aagcaatccg caatgggaaa gctagctatt atacaccaac
    30901 agcaggaatc ccagagcttc gacaggcaat cgttgattat ctgaagaaaa actatggact
    30961 tgtctatgaa ccttcacaaa cagtcgtaac agacggagcg aaatttgctt tgtataccct
    31021 tttccaaacg atccttgacc cgcaagatga agtcatcatt cctgtgcctt actgggtcag
    31081 ttacggtgag caagtaaaac tggcagaagg acttcctgtc tttgtgaaag gggaagaatc
    31141 caatagctgg aaagttactg ttgagcagct agaacaagca cgttcagaaa aaacaaaagc
    31201 cgtaatcatc aattctccat cgaatccgac tggaatgatt tatagcaaga atgaactgca
    31261 ggcaatcggt gaatgggcag taaaaaatga tattttgata gttgctgatg atatttatgg
    31321 ccgtttagtt tataatggca atgagtttac tccaattgca acgatttcag aagcaatcaa
    31381 aaatcagacc atcatcatca atggcgtgtc taaaacatat gcgatgacag gctggcgcat
    31441 tggttatgcg gtggggaatt ctgagatcat caatggaatg attgctgttg cttcccagtc
    31501 aaccagtaat ccgacagcag tcagtcaata tgcagctgtt gaagcattga aaggcgagca
    31561 agatacagtg gaagaaatgc ggaacgcctt tgaagaacgc ctgaatacgt tatatccgct
    31621 tgtggcagaa ctgccgggtg tttctcttga aaagccacaa ggagcttttt atctttttcc
    31681 taatgtaaaa gaaacgctac gcatgtgtaa atatgaaaat gtcacacaat gggtagaaga
    31741 tcttttagaa gaaacagggg tagcgcttgt gactggcgaa ggctttgggg caccagaaaa
    31801 tgtccgcatg agttatgcca ctgatcgcgc tacgttagaa gaagcggtac gcagaatcaa
    31861 gcaatttatt gagagtaaaa gtcaaaatta atcctgaaga aattcaagga atataaaagg
    31921 agagtcatac gtggaacaaa ttcaaatcat cgattcgaaa aaccatgtgg gagaaacagt
    31981 aacaatcggc gcttgggtcg caaataagcg atcaagcgga aaaatcgcat ttttgcaatt
    32041 aagagacgga acagcctttt tccaaggtgt agttgtaaaa agcgaagtgc cggaagaagt
    32101 tttccaaaca gctaaaaatc tacaccaaga aacatccgtg ttagtaactg gagaaatccg
    32161 tgaagacagt cgttcaaaat ttggttatga aatcggaatt acaaatgtgg aagtggtaag
    32221 cgagagccac gattatccga tcacaccaaa agaacacgga acagacttct tgatggatca
    32281 tcgtcattta tggctgcgtt cttcaagaca acatgcgatt atgcagatca gaaatgaaat
    32341 cattcgtgca acgtatgaat tctttaatca acatcatttt gtcaaaatcg atccgccgat
    32401 cttgactggt tctgcaccag aaggcactac ggatctattc gaaacaaatt actttgatca
    32461 aaaagcttat ttatctcaaa gtggccaatt atacatggaa gctgctgcta tggctttcgg
    32521 aaaagtcttt tcattcggtc cgactttccg tgctgaaaaa tcaaaaacac gacgtcattt
    32581 gatcgaattc tggatgatcg aaccagaaat ggctttcact catcaagaag aaagtttaga
    32641 agtacaggaa caatatgtag cattcttagt acaaaatgtt ttagatcatt gcgaatacgc
    32701 attagatgta ctaggacgag acaaagaagt cttaaaaact tatacaaaat tgccattccc
    32761 tcgtattact tatgatgatg cgatcgaatt actgaagaaa aatggatttg atgacatcga
    32821 atggggcgaa gactttggtt cacctcatga aacgttcatt gcgaattcat tcgaacagcc
    32881 tgtctttatc ctaaactatc caaaagccat caagccattc tacatgaaac cacatccaac
    32941 aagagaagac gtggtgatct gtgcagatat gatcgcacca gaaggttacg gtgaaattat
    33001 tggtggaagc gaacgggcaa cagactatga tttcttgcta gaacaaatcc gtaatcacgg
    33061 tttggatgaa aaagaatatt catggtatct tgatctacgt aaatacggtt ctgttccgca
    33121 ctctggtttt ggtctaggcc ttgaacgaac agtcacttgg attgctggta tcgaccatgt
    33181 gcgtgaagct attccattcc cacgtttgtt gaaccgaatt tatccataag aggttgtgaa
    33241 atcagtgcct tgacctgagt attagagaaa caaaacggaa aaatagcttc tcatatttta
    33301 ctgttttttg agctaatatc gagggtcagc tgattgaaca ccgtttatat caagaggttg
    33361 tgcgtctgcc gttcagctcc gagtcacaag gagtaatttc aaaaaaacat tttttcatgt
    33421 ttgatgacta ttgtgacttg tgaccaagga gttggccgga gagcaacgtt tatttaaaaa
    33481 aaagaactgc gaactctcgc agttcttttt ttttaaatac gggataaagg aagtttccag
    33541 cagataagag aagaaagcaa aagaaattgc tatttgattc acaaggttct ataacgaaat
    33601 cgcaatcaat caaacatata caaagaaggt tattcaaatg gaaatctgat ttgatgatgc
    33661 ttctgctgtt gaaataaagc agcatctagc acctgaaaac aaactaatag taacattcga
    33721 agatggagta ggtccgtatt ttcagcatgc aatgatccat atgtaagttc aattcaccct
    33781 tgaaatcatc ggaccagata aagttatggc caatctatcc caagcaatct agaagagata
    33841 ttagtaaagg aacatttact ggaagtctta gatgagccta taaatatatg ttctgatggc
    33901 gtcatcaagt ggtctgattg acggaagctt aggtgtgatc gatctctctg gaccaaacaa
    33961 tgaaggaatc aaggggaatc cagcacgtta agaaaaaaat agcataaaaa actagacact
    34021 tgatgagcgt tcattcaatt gtttgaaggg actaggctgg tgtcattttt tatacatata
    34081 ttttctcttt cgtcttgata gggcttatgg gataatataa ggaagaaaat catcgtttta
    34141 ttcaaaaagg agtttttgac aaatgaaaaa acgtatatta tttatcggtg acagcattac
    34201 agacgcagga agagaccgag cagaagcaac tgatctaggt aaaggttatc cacttttgat
    34261 tgccggaaaa ctaatctccc aatatccaac gatggaattt gaatgcatga accgtgggat
    34321 aggaggagac aaaattgcag atctgcaagt acgctggcga gaagattgtt tagctttaca
    34381 acctgactat gtatcgattt tgatcggaat caatgatacc tggcataata ctagcgatca
    34441 ggctatattc ggaactgaga aatctgctca gcaatttgaa tccgtttatc gaaaattact
    34501 aaatgaactt caaaaagcgg gaatctgcaa catcatcttg atggagccgt ttgtacttcc
    34561 tataaccgac gaccgtcatg gctggcgtaa agacttagac cctaaaatcc aaattgttcg
    34621 taaacttgct aaggaatatg gcgcattgct ggttccttta gatggcatat tcaatgcttt
    34681 agggattgct tatggttttt cgaaatatac aagagaagat ggggtccacc caacgattgg
    34741 cggacatgag atcattgcac aaagctggat agatgcaatt gccgcagtcg atttttttag
    34801 tggagataaa aatgagcgaa agaaaagtga gaaatgatgg aaatacggtg ggcagtaaaa
    34861 gaagatctac ctcaaatcaa tcagctgatt actgatcatc cagaatcatt cgtaaaagca
    34921 aaaacggcag ctgacctaaa tgataagaat aaactattgc tcgttgctgt aacaaaagaa
    34981 aaacggatta tcggttttgc gcaagcggac gtgcaacagt cagaagaacg ggtgggtcag
    35041 cgaccatcag gaaaactaat ccaacacaca ttagtaacaa aagaaaatac ggaagaaatc
    35101 caaaaaaaat ttgcagaata tttgatgctg gaagctaaaa aaagaagaat aaaatcaatt
    35161 tttgcccagt tttaatcaaa aacagtaata gaaggagaaa tacaatgagt tgtttaggaa
    35221 atgtcatctg gtttatattc ggcggactaa tcggtggtct tagctggtta gcagcgggga
    35281 tactttggtg tatcacgatc ataggtatcc caatcggtat gcaatgtttt aagatcgcct
    35341 tcctttcttt tgcgcctttt ggcaaagaag taatttatca ccagtctggc atgtcattga
    35401 tcgttaatat catctggctg atcgtttcag gcctaccaat tgctctcgca catgtcgtca
    35461 gcggcatcct tctatgtatc acgatcatag ggataccttt tgccaagcaa tctttcaaac
    35521 ttgctcgatt ggcgttgatg ccattcggtg cagaaattgt ataaaagaga ataataaagg
    35581 ttagataaag catagagtca ggtgggatac atgtatttca cctgactcta tgcgttttcc
    35641 tgtataagtt cttccatgtg acttttacgt cttttattta tatggggtta ctgtttatgg
    35701 aaagttgaaa taattttcat taaagaactc tttctatatt tttgaaagcg tagtggagct
    35761 tcgtatatct gggaaggaca ttttgcaggg attgaaaaaa gatattgtcc tatcgaaaaa
    35821 ttaatgaatt tagacaaatt gccagcaaca ggcttcaaac ttagttgcta tcctgtcaag
    35881 atcaagaagg caagtgcggg ttggataaga gcagtggcaa tgatcgaaga gaaaaagaaa
    35941 gaataagcca aaacatctgt atggaagaaa ttttccgatg cagatgtttt tcattttttc
    36001 tttcggatac cttgacatat gaataaccat tcatttataa ttagttatga agagatattc
    36061 atatgttgag gcgaggaggt cattaaaatg aaaagttaca gattagaagg tttagattgt
    36121 gcgaactgtg caatgaaaat cgaaaaagga gttcagaaaa tcaatggagt caaggaagca
    36181 acggtaaact tcacttctgg aaaactaacg attgacgcgg aggaagatca tttggctacg
    36241 attgaacaag aaacaaaaaa agtagtgaag gaattagaac cagatgtcaa agtaacagaa
    36301 atagataagg aaaaagtatc tgaacatgga aatgaaaaag agagaaatac gctgttccgg
    36361 attttatttt cccttgcagg tatcgcttta ttgctattat ttgattttaa tgaactaatt
    36421 cgcttgatag ggtatttatt gatttatctg ctgataggat atgatgtagt gaagaaggca
    36481 gtgatgaata tcgtcaaagg aaaaatcttt gatgagaatt ttcttatgtc tgttgctacg
    36541 attggcgcta tgttgatcgg tgaatatcca gaagccgtag cagtgatgct attttatcaa
    36601 atcggcgagt atttccaagg attggcagtc agtcattcaa gaaaatcgat tcgcgaattg
    36661 atggcgatcc gtccagaaac agctcacgtg cagacggcgg aaggattgat gaccgtgaac
    36721 cctgaagacg tgctgatcgg tcagtttgta cttgtgaagc ctggtgaacg tgtaccgtta
    36781 gatggaacga tcatcgaagg agaatcatta gtcgatacgt cagcacttac aggagaatca
    36841 gtccctaaat ctgtatatgt tggtgaaaca gtattaagcg gatttatcaa taaaaataaa
    36901 ccatttttag ttcaagtaga aaaatcttat gaaaattcta ctatcagcaa attattagaa
    36961 ttagtagaaa atgccagcag taaaaaagca ccagctgaaa atttcattac taaattcgcg
    37021 cgctattaca caccagtcgt tgtaggattg gccgttttat tagctgtttt acctccactt
    37081 gttgtttctg gagcggcgtt ttctgaatgg atctatcgag cattgacatt tttagtgatc
    37141 tcatgtccat gtgcattagt gatttccgtg ccacttagtt tctttggcgg tatcggagga
    37201 gcaagtaaaa tcggtgtatt agtaaaaggt tcaaattact tggagttgct tgcgcaaacc
    37261 gagacagtgg tattcgataa aaccggcaca ttaacgaaag gcgacttttc gatccagacg
    37321 atcgatacaa aaatcgatcc aaagatattc atgcaatata ttgcgagtgc agaacagttt
    37381 tctacacatc cgattgctca atctgtttta gaaggctacg caggtccgct tttaccaaca
    37441 gctaatatcc aggaatttgc aggtgaaggg atcttagctg aagttgatgg caaacaagta
    37501 ttagtaggta atcacaaatt gatggaaaga tttgaaatct cttttccttc aagtcaagaa
    37561 atcggtacat tgctttatct agcaatcgat caaagttaca gtggttatct tgttattgca
    37621 gataccttga aagaagatgc cgtagacgca ttggttcaat taaaacaggc tggtgtgaaa
    37681 aacacagtca tgttgacggg cgactcgaaa aaaattgctg atcatatcgg gaagcaagta
    37741 ggcgtggaca agatatatag cgaattgctt ccagaagata aagtgcagcg gttagaggaa
    37801 atcttacaaa gtaacaataa aaagaccgca tttgtcggag atggaatcaa cgatgcaccg
    37861 gtgctagcaa gagcagatgt aggaatcgca atgggtggat tgggaagcga tgctgcaatt
    37921 gaagcagcag atgtagtcat catgaatgat caaccatcga agattgcaga agcaatccat
    37981 ttagcaaaga aaacattgaa aatcgtcaaa caaaacattg tctttgctat tgggataaaa
    38041 attttggtac tttccttagg tgcttttggt tttgcatcaa tgggagatgc agtatttgca
    38101 gatgttgggg tgacagtatt agctgttttg aatgccatgc gaagcttgca tgtaaaaaat
    38161 aaacgttgat caatgaagag gacgaatgaa cgtcctcttt tttttatatc cttttcttaa
    38221 tgaaaaatgt aaagaaaata gataggatta taaaataact aatgttttta attttgcaga
    38281 aaattcaaaa tagcttgaaa ttcaataagg tcaatgaaat aaaagaaaat aacttatttt
    38341 tttgatggaa aaaatatgaa aaaacgatgt gtattaaata aaataataca cacataaaat
    38401 aacactgtct tgtaagtttg ttaaaaattg aatatgatta catctggata acaaattttt
    38461 atcataaagg agagaacgaa tgaacaacaa acaattacgc tggtttactg ttgcgttaat
    38521 tgcatttaac atggtttggg gcatggggaa tgtagttaat aactatgctc agcaaggagt
    38581 atcagttgtc acctcttggc tattgatttt ggccatttat tttattccat acgcattgat
    38641 cgttggacaa ttaggatcag ccttcaagga tagtaaagga ggcgtcagtt catgggtcga
    38701 aaatacgaca tctaataaac gactggcata ctatgccgct tggacttact gggtcgtcca
    38761 tattccttat ttggcacaaa agcctcaagc aattctgatt gctggtggtt gggcagtcca
    38821 aggaaatgga aatatcgtaa atacgatgtc agtacaaatg gtagcagtta tttcacttat
    38881 tatatttctt gcctttttat atttatcaac aaaaggactt tctactttga aagtgattgg
    38941 cggactggcc ggaacagcga tgttcgtcat gtcgatctta tttattcttt tagctgtaac
    39001 ggcaccgttc atcaatccaa cgatggaatt tgccacacca catatgaatc aagtgaaaac
    39061 atatattcca acatttgatt tcagttattt cacaaccgtt tcgatgctag tatttgcagt
    39121 tggaggggcg gaaaaaatat ccccttatgt caactctaca agaaatcctg caaaggaatt
    39181 tcctaaaggg atgatcttct tagcagcaat ggtagggatt tgtgctgtat taggctcagt
    39241 cgcaatgggt atgctgtttg ctagcgataa tatccctaaa gacttgatgg caaatggggc
    39301 atacagtgct tttcaaattt taggtaatta ctatggtatc ggtaactcat tgatgatcat
    39361 ttacgcattg acaaacatga ttggacaaat ctccgcatta gccttttcaa ttgatgcacc
    39421 actaaaaatc ttgttagcag atgcggatcc agaatttgta ccaaactggc tacgtaaacg
    39481 taatcgtaaa ggtacattgg tgaatggtta tcttctgaca ggtatactag taggaatcat
    39541 tattttgttg ccgatctttg gtatcaaaga tatgaatgaa ttagtaaaat ggttgacgaa
    39601 cttgaactca gttgtcatgc caatgcgtta cctatgggtg ttcttggcat atatgatgtt
    39661 gaataaagct tataagaaat tcaattctga atataaattt gttaaaaatc caaaaatcgg
    39721 ttttatcttt ggtgcttggt gctttgcctt cactgcgttt gcatgtatcc taggaatggt
    39781 gccaaaagta gattatactg ctgatcctaa agcatggtgg ttccaattat tcacgaatat
    39841 catcacacca attgtcttga tcttattagg tatgatcttg ccagcaattg ctcgtcgaga
    39901 caaaaacaaa gaaattactg aaccagaaac agtggttcag ccttaaaata gaaatacttg
    39961 ttacaagagg ttgtgacaga agctacttct ggaacaccgt gaatcagagg ttgtgaaaaa
    40021 gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agctcctcat attttgccgt
    40081 tttttgagct aatgctgaag gtcagctgat tgaacaccat ttattcgaaa caagggactt
    40141 tgacacgact tttgtcacag tcccttgttt ttttgcggcg ttataagtgg tatgatcgaa
    40201 ccaaattcca aagagaagtg agggagaaga atgaagatca tcgtgattgg acaaagtggg
    40261 tcgggaaaat cgtccctcgc aaggaagatc aaagaaatca ccggctttcc gcattcaggc
    40321 aaaaaagtgg tttttgcaag aacagcaatc gtttatagta tctaatccgt catggatcgt
    40381 tgaaggaaat tatacttcta cgttagagga aaggattaaa gaagcagata agatcatttg
    40441 gcttaaagtg ccgcgttatc tcgccatcta ccgagtagtt tggcgttctc taaaaagaaa
    40501 gataaacaaa aaatctcggc cagatatgcc tgaatcattt tcggagaaac taaacaggaa
    40561 atacttagaa tttttatctt ttatctggca ttttgaagga aacaatgaac caaaaataca
    40621 gcagctgatc caagtagcta atgcaagaaa taaactgatc atccttaaaa caagacgaga
    40681 gaaacaagaa tttttaacta aattgaaaat agagagcaaa aataattgac agtttttgtt
    40741 tacaagcgta atctgtattc ataagctaat gattacattt gtaaacgaaa ggtgtgaaat
    40801 aaatggaaaa agtcaaaacg ccagtaaaga tcagtgattc agagtgggag atcatgcgtg
    40861 ttatttggac acttggacag acgacagccc aagaaatcac gcagatctta gctgattcga
    40921 tggattggaa accagcaacg atcaaaactt tgctgggacg tttggtaaaa aaagaagtga
    40981 tctggacaga acaaaaagga aaaaaattca tttatcatcc agctgtctcg gagatggaaa
    41041 cagttcgcag tgcgacggaa aatctttttt cccatatctg tgcaaaaagg attggagaga
    41101 cgattgcgga tctagtagaa gaagctactt tgactcaaga agatatcaat gagattttga
    41161 aacaactgga acaaaagcaa ccagtagata cgatcgaatg taactgtatc ccaggtcaat
    41221 gtgaatgtaa acaacatcaa taggaggaag aaaaaatgaa acaacaattt tcaatcgaag
    41281 gtatgagctg caatcattgt gcagcaagag tagaagaaac agtaagcgca cttgacggcg
    41341 tgcaaaaagt gaaagtaaat ttgaaaaaag caaacggtac ggtcaagttt gatgaaacaa
    41401 aagttcaatc cgaaaagatt tgtcaagcaa tcaatggatt gggttataaa gcagaggtga
    41461 tttgatggca acagcagaaa ccaaaatgga tacgttcgtc attacaggta tgacttgtgc
    41521 aaattgttct gctaggatcg aaaaagagct gaacggacaa cctggtgtgg tcaatgccac
    41581 agtcaacttg gccactgaaa aagcaagtgt caaatatgaa gggacgacca ctgaaaaatt
    41641 aatccaaagc gtagaaaaca tcggttatgg tgcaattttg tacgatgaag cccacaaaca
    41701 aaaaattgca gaagaaaagc aagcgtattt gaaaaaaatg ctattcgatc ttatattgag
    41761 tacggttttg actttgccgt tgatgttgtc aatgattgct atgatgttag gaagccatgc
    41821 ggcgattgtt cacttttttc attttccaat cgtccagtta gtgctgtctg ctcctgtcca
    41881 attttatgtc ggtgctcgtt tttataaagg tgcgtaccat gcaatcaaga cgaaagcacc
    41941 aaatatggat gtattggtag caattggaac atcggctgcc tttgcattga gcatttacaa
    42001 cggatttttc cgtggacacc cccaagatct ttattttgaa agcagcagta tgatcatcac
    42061 gctgattttg ctcggcaaat atttggaaca tacagcaaaa acgaaaacag gtaatgcaat
    42121 caaacaattg atgtctcttc aaacaaaaac cgcccaagtg ataagaaacg ggaaagaaga
    42181 aacgttagcg attgaagaag tagtcgttgg tgatcaacta gtcatccgtc ctggtgagca
    42241 gattccagca gatggtcgaa ttatttcagg aagcagtgcg atagatgaaa gtatgcttac
    42301 tggtgaaaat cttcctgtag aaaagaatcc ggatgacacg ctttttggcg ggacaatcaa
    42361 tacgaatggt ctgctacaca tggaagtcac acaagtcggc aagcagacag tattagcgca
    42421 aatcattcaa atggtggaag atgctcaagg gagcaaagcc ccaatccaaa aaatcgcaga
    42481 tcggatctca gggatttttg ttccgatcgt attagtcatc gcgtttatca cgctcatagc
    42541 tacaggtttg atcactggtg attggcaact cgcattgatc catagtgtct ctgtattagt
    42601 cattgcgtgt ccatgtgcat taggtttagc gaccccaaca gctattatgg taggaacggg
    42661 tgttggtgca cgaaatggaa ttttaattaa aggcggagaa gcattagaag cagctgctca
    42721 cttagacagc attgttttgg ataaaactgg aacgattaca gaaggaaagc ccaaagttac
    42781 agatctagtc ggttcaaaag aagtcctatc tattttttat actttagaac aagcttctga
    42841 acatccacta ggtaaagcaa tcgtggaata cggaaaactg caagaagcag caacatacga
    42901 tatgattgat tttacagccc acccaggtgc tgggatcagc ggtacgatta atggagtacg
    42961 ctattttgcg ggtacacgta aacgattaat tgaattgaac ctatcatttg atgaatacca
    43021 agaacacgcg ttagaattag aacagcaggg aaaaacagtc atgtttttag ctgatgaaaa
    43081 gcaagtgatc gggtggattg ccgtggctga tcaaatcaaa ctagaagcca aacaagcaat
    43141 caagcagctc cagaataaag gattagatgt ttttatgctg acaggagaca acaaactggc
    43201 agccgaaacg attggtaaac aagttggtat tgatccaaag catatctttg ctgaagtact
    43261 tccagaggat aaagcagcct acgtagaaaa acttcagaaa gatggcaaaa aagtcggtat
    43321 ggcaggcgat ggaatcaatg atgcaccggc cttagcatta gctgatgtcg gtatggcaat
    43381 gggaagtgga acggacattg cgatggaaac agccgatgtg acgctgatga atagcagttt
    43441 ggcatccatt gcacaaacaa tcgagttgtc taggttgaca ttgagaaaaa tcaaacaaaa
    43501 tcttttctgg gcttttgtct ataatacgat tggtattcct tttgccgcac taggcttttt
    43561 gaatccaatc attgcaggag gagcgatggc gtttagttcc gtcagtgtat tgttgaactc
    43621 cctgagctta aatcgacaca tgaaaaagta agagaaggga tggagtaaaa atgaatggga
    43681 acgatatgga gaaaaaacat gagcataaaa aagaagtagt aaacaaagaa agtacagaat
    43741 ctcatgaaaa acagatggag atgaaacatg accatagtca gatggatcat tccatgcaca
    43801 tgggacataa ccatggagat atagcaaaca gcaaacaaat ggaccattcg atgcacatgg
    43861 atcacgaaca cggcggcatg gatcattcca tgcacatggg gaatttcaaa caaaaatttt
    43921 ggctatcgct tatcctagct atcccaatca ttcttttttc accaatgatg ggaatggaat
    43981 ttccatttca agtaacgttt cctggttctg attggctcgt attgatctta gcaactatac
    44041 tatttatcta tggcggtcag ccatttttaa gtggagctaa gatggaattg aaacaaaaaa
    44101 gtcctgctat gatgacactg atcgcgatgg ggattacagt tgcatatatt tatagtgtct
    44161 attcgtttat cgcaaatctg ctcaatccac atacacacgt catggatttc ttctgggaat
    44221 tggctacttt gatcgttatt atgctactag gtcactggat tgaaatgaat gctgtgtcga
    44281 atgccagcaa tgctttacaa aaactagcag aattactgcc agaatcagtg aaacgcttga
    44341 cgaaagatgg aaaagaagag acagtttcat tgaaagaagt aaacgaaggt gatcgtttga
    44401 tcgtccggtc tggtgataaa atgccaacag atggtgtaat cttgaaaggt gaaacaattg
    44461 ttgacgaatc agctgtgact ggcgaatcaa gaggaatcaa aaaacaagcc aatgacaaag
    44521 tgattggcgg ttctatcaat ggggatggaa cgatcgaaat cgaagtaact ggaactggtg
    44581 aaaatggtta tctagcgaag gttatggaaa tggtgcgaaa agcacaagga gaaaaatcca
    44641 agctagagtc attatctgac aaagtggcaa aatggctgtt ttatgtcgca ttaattgtgg
    44701 gtatcctggc attcatcgcc tggttgttct tgacagatct tcctaatgca ttagaacgta
    44761 tggtaacagt ctttatcatc gcctgtcctc atgctctagg tttagctatc ccattagttg
    44821 ttgctcgctc cacatcaata gcagctaaaa acggattact tttgaaaaac cgcaatgcac
    44881 tggagcaagc caatgatcta gatgtgatca tgctggacaa aacaggaaca ctaacagaag
    44941 gaaaatttac tgtaacgggt gtcgaagtac ttgatgatgc gttcaataaa aatgaaatcc
    45001 ttcaatatct aggagccttg gaagcgaatg ccaatcatcc attggctgtt ggcattatga
    45061 attatttgaa agaacatgaa atcaagcctt atcaagctga aaatctaaaa aatctttcag
    45121 gagtcgggct agaggcaaca gtcaaaaaac aacaagtgaa aatagttaat gaaaaagaag
    45181 ttgagcgtct cggattaaac gtagaacaag cattattgaa accttaccaa gaacaaggaa
    45241 ataccatcag cttcctgatt ttagaaaatc atctagctgc aatcgttgct ttaggtgacg
    45301 tagtaaaaac agaagcaaaa gaatttatcc gaacattgaa agaaagaaaa atcactcctg
    45361 tcatgcttac tggtgacaat aaaaacgctg cccaagcagt agcagattat ttagggatcg
    45421 aagaatatta tggcggttta ttaccagatg ataaagaagc tgttgtccag aaataccttg
    45481 accaagggaa aaaagtaatc atggtgggtg atgggatcaa tgatgcgcca agtttagccc
    45541 gtgcttcaat cgggatggca atcggtgcag ggacagatat cgcaatcgat tctgcggatg
    45601 tcgtattgac aaacagcgat ccaaaagata ttttgcattt tcttgatcta gccaagcaaa
    45661 cgcgaaagaa aatgatccag aacctctggt ggggggcagg ttacaatatt cttgcaattc
    45721 cgctagcagc cggaattctt gcgccaattg gtatcatatt aagtccagca gttggtgctg
    45781 tcttgatgtc gttaagtacg gtagttgtag ctctaaatgc tttgacattg aaaatcaaat
    45841 aataatgaaa aaaggttgtg acaaaaaacg tgtcacaacc tttttttcga gtaatcgata
    45901 ctcaatcagc tggttctcgg cattccagaa gtagattctc cagaaatttt atcttatttt
    45961 ttgaagcggc tcccttttgt cagaattttt ttgtataccg tttttcttat tatacgcgtt
    46021 atttctaaat aggttttttt atcgaatttt accggatctg cttgtcgtcc atatatttct
    46081 gcttcatatt gagtagttaa tcgaatgaaa cgcagatcta gcttgaattc ttgttctatt
    46141 cgcaggctat attcctgcaa agattctgcg ggtttgcgcg gtaccaatgt ttccaaaggg
    46201 ttaagaagtt tttgatatgc aatcgtatta gtagaagcaa aaaaatgaag atagataaga
    46261 taacttgtcc agcgcagaga ttttctgaat aacaatagaa agaacaacaa gacggataaa
    46321 cacactccaa ggctgaataa gacgatttta taagctggtg tgagtgttgt ttcttctgat
    46381 ggaatgacct cagatctctc actggaaata gtttctgcca tattttcgct ttcatttggt
    46441 tgttcgatag caggctggac ggtttcggtt gtttcagcaa ctggttgtga acccattgga
    46501 ttggcaaatg aaggggttgg ttcaaatgga agccagccaa atccaggaaa gtatacctct
    46561 ggccaagaat gagcatgact attgagaatg ctgtattcct cattcccttg attatctgtg
    46621 cctgttctga gcccatttgt aaaacctttt gtccagcgag ctgggatacc aagtgttcga
    46681 agaaggacga ccatagaagt tgaaaagttg tcacaatagc cgacttttga atcaaagagg
    46741 aaataatcca cataatcgtg ttctttcgga gtatgcccgg tatccgtttt ggaatagcgg
    46801 aaatttccgc ttgtttttaa atactgttag acagcttcga ctttacgaat taaagttgct
    46861 tcatttttgg tcaattcgac cgccaagtcc tcaatacgtt taggcaaact ttttggcaat
    46921 tgaagatttg tctgctgatt ttctaaatca tcagctttta actgactgct ttttaattct
    46981 tctctcgtaa aaactaacct ttctgtttgt aaagttgcag aacgtgtcga ttcattcggg
    47041 acaggaatga atcgctggct ttcaggcaaa tagtaaagcg tttgtccact agctaatgaa
    47101 aaatcagtca aggtagtatt tccataagga taaggtaaga aagcttgttt ttttagggtc
    47161 aatgtaatgt tttctttgtc ttctataacg gtttggtagc tcgtgatgtc aacagggaat
    47221 tcttggattg gttggatggg tgcgtcctga tcttctatcc atccagaacc ggtgtaatga
    47281 tttttatttt cgatcttcca atagccattt cctttttggt tcgctgtaaa aacaacttcg
    47341 ccattatcat ataaaggtcc accaagctgt tcatcattct cactgaagcc tgttcttgca
    47401 cttccggtta attgatactc atggagtgtg tcataaagcc cttcatcatt aagatattct
    47461 cggatcggtt cactcgtttc ctcgagagat tggtgtactt tcggaagata taaaggcaag
    47521 atagcgctaa aagttaagaa acaaataagc agaactgtag tcaatgactg gaatcgagtg
    47581 tttcccgaca acaaccggat ggagaaaaat tgccttgctg ccagatacac gaacaaaaca
    47641 gttactaccg caatcatttc gtacagtaaa tcttttttcc gaaaaacggt aagcatcatg
    47701 aagtaagcca tcaaagaaag aaagggtgcg taccaatagt cgtaatccac ggtcaatgta
    47761 ataaataaat agatcagcaa cattatgaga aaaaaagctg tcttttctgg caaataataa
    47821 agtttacctt gggatagatg gaagaattcc tctgtaactg acgaaaaaaa ctgtaagatc
    47881 cataatatgg aaaatgtctt cgaaggaaaa tataaaaaca aacaacctat cattactggc
    47941 aaagaaagca gcaaacgtac ccatttttta ggaatacagc agacaataag gcataggaca
    48001 ccaatagtta ggataagaag gtagctgttt tgcaattgat tagctactac aaatatcgga
    48061 gcacctacac ttagtatcac aaaggagtaa acgccacgca gccatttttc ccgtttaatc
    48121 gtcaatccat tcacctcctg gcaatgaaca tatcttgtct ttttcttgga ccatgagttg
    48181 tttttctacg aaataaacaa gataagccgt tcgatctttt gacaatgttt caatagcttc
    48241 tgagacttca ggagaattct caggaacaaa gaggacgatt tgtttttttg catatttttt
    48301 caagtgtgcg aaaaaagctt tctcatcgcc agcttccaaa gaagcaaaat aggtatgacc
    48361 ggggtctttg ccatgaaagg cgcggttgcc taatatcaga agttccggct gcttttcttt
    48421 tctcaaatgc cataaagaaa aatacaagtc tagcagaggt tcaaattttg gactgggcga
    48481 accccaaaag caaaaaagag gagaacattc ttcttcttga taatcgtatt cacggtataa
    48541 ccactcttga gtatgagcag acaatttcca atcgattcgg ttgaaagaat cacctggacg
    48601 ataagcgcga tgttcccgaa aatcaagacc tctttggtac gcgttcagct gctgtttttt
    48661 ttccagaact ctctgtaatt tttctgcttg aatcttacaa aaagcaggca gaacagtcac
    48721 aggaaaagag atctctcttt ttgcactttt tctgaaaagt cctagactat cataggctgt
    48781 aaaaatgaca gttaatgttt catggcatcc tctttctttt ggctgccaaa gcaattggac
    48841 ttcttgcggt ttatttgtaa gaatcaaaat ctcttgtttt tttctgcaaa gattgctggg
    48901 aagccgattt gtaaacgagg gagaggtaaa gaaaaagttc tcttttttgc tatggaaaaa
    48961 ataacctgca tacttttttt gcgataaacg agtggctgca atgtacaagt aaccgaaagt
    49021 ttctttagaa agctgcttaa tgaaatcaag ctccatgata cataaagagt aaaaaaggcc
    49081 aacaagaacc agcctaaaga attgttgaat acaagagcaa aaagcacaat gatgaaatat
    49141 accagaaata acacaaaatt aatgaaataa tctttgattt tttccattgt ctacctcaat
    49201 ctacctcaca ggaataggaa tttgtgcaac aagagtatga atgatttgtt tcttttcgtc
    49261 ttccttcaat gaacgatcgt aaaaaatcag gcgatgggaa aacacgtaag gaactaaatc
    49321 ctgcaaatct ttgggcgtga cgtaatttct cccatggatt aaagcataag cttttgctgc
    49381 ctgaataaag gaaaggcttc ctcgtggact gatgccaagc tggatggcag catgacttct
    49441 tgttgctgac actaactgca aggcataaga tgccacttcg ggactgacaa agacattcgc
    49501 cacatttgtt tttatttctt ctatttcata actgttcaat acttcattta agttatccaa
    49561 cactttttcg aatttatcca aaagtagcaa aagttcgtca tcaaaagaag gataaccgat
    49621 ttgtagtcgg aataaaaaac gatcgagctg tgcttccggc aaaggatatg ttccttcgta
    49681 ctcgattgga ttttgcgtag ctaagacaaa aaaatgattg tctagagggt atgtattgtt
    49741 gtcgatcgtt gcatgattct cggccattgc ttctaaaagt gctgactgag tacgaggagt
    49801 cgtacgattg atctcatctg ccaacaagat cgtggtaaag atcggtcccg gacgaaattc
    49861 aaactctttt gattgtgaat tgtatacgga aaaaccaaga atatcactag gcagcagatc
    49921 aggagtacat tggactctgc tgaaggttcc ctgtacagct tttgctagtg ctttgaccag
    49981 taacgtttta cctacaccgg gaatatcttc gaataagaca tgaccacctg ctaatagggc
    50041 cacgatcatc aattggatag ttgaacgttt gccgatgacc gctttttcta attcttatat
    50101 caactgatcg attttttgtt tggattcttc tattttttct tccattttca catcaccttc
    50161 tcataaaatc gctatttatt tcattgtaat ggaagggaat acatcctaca agcaaagtga
    50221 tcttattatg tttttttaaa gtacattcgt tttgaaatga gaatacgatt aaattgagta
    50281 aaataaaaaa taatgaggaa gatttgcgta tttaaataaa tagaaaggga gttttttatc
    50341 tttttaggag tgaactcatt ttgatcaaac aatatgttta gaaatcaggt gagaaatgat
    50401 cgatgagatg gcaaaaacta ttatcaaaaa atgcacagaa tttattttca ctaatcaatc
    50461 taatgaaaga tttgatctgg cgaaaagctg atttagctga agaactgaaa atcgatgtta
    50521 aaacagtgga tcggcatctg aaacgtttgg cggaaagcga ctgtcccatt cagctgattc
    50581 ttcataagaa aaccgttcag ttagtttatg atcaaggata tagcgaacct tatctcttgt
    50641 ttgtttttct gatccaatca cccccgtttt gtttactgaa aaatctgcta ttaaatgaac
    50701 cttacgagca gaagtacgat cgttctacgc aagagcgatt gagaaagtgg tttggcgagt
    50761 accacctttc tttttcttat aaaaaagcac agatctttgg ttcagaaaga aaagtaagat
    50821 atctgctgtt ttactttttg aaagattttc cgcatttttt tgcagatgat ggagagcagt
    50881 ccttttctga gatgttcatc caattattga agcaaagaaa ggatccggca gaaatcgaga
    50941 ttttaggaga cagtcgaaca ctattctttg aattgtttcc tgaattgcta tttagagagg
    51001 agcagatttt gccaataaaa gaaaaaaatt atctagcaat tttgcgcttg agctgtaacc
    51061 agattactga aaagcggtgg aaacggatag aagagacaca agactatcgg aaattgacga
    51121 ttgtgactga aaagcttgat tcgctgtttt ggctgaatag cgaatttgag agcgaaagca
    51181 aatcaaagct gataaaagta ctttaccaag aatggcttcg ttacgtaatt ggtatccatg
    51241 accggctgat agaagaagtt cgttattatc aggaaacatt agaaaaattt cccaattcca
    51301 aagataaact atatcaattt cggctagcga tccaacaagc acttgcacac cccgcagtag
    51361 agtgggggat gcttttactg aaaacactcc aggaacgagg gtatttagat gtccaccctc
    51421 ctgaagtgaa aatcgtcttt ttttttcgct atgaacacca agaagcaaga agacttgctg
    51481 gggtactgaa caaaacgcta agacatcgaa aacggatctc tatacatgta tgtactcgaa
    51541 ataagccgcc tcggtatgca gatctagtga tcaccgatca tttttttcca ctagaccata
    51601 acgaagatca aaaaacgtat ctgtaccatc cttcatttgg tatcgaaaga cttcttaccg
    51661 atatcaatta ctggttaggc aaaaaccgat aaaacctttg gaaaaaagat caaaacaagt
    51721 cagtctgtgc tataataaaa agcgattagt aaaggaggaa atccattgga attaaagaaa
    51781 ttagaagttc ccacatcttc gatcacagaa gtgaaacgat cgccgatgga tgtatttgct
    51841 caagcgcgag acgcgggaac tggcgtatat atatttaatc gtgaaaaagt agctggcgtg
    51901 atgctgaccc aagaacaata caaaacgctc ttgcaagaac tagatgtcct tcgtcaaaca
    51961 gtgaaagtgg agaggaaaga accaaaagaa ataaacgaag aacctattaa caaaacagct
    52021 tccgtcgtat ccaatgctga agggatggaa gagctggccc aaacattaca gcactcgctc
    52081 attactggtg tgacgattac agcaaaaaat ctagatgaac gaatggtagc actaggtttt
    52141 attacgaaaa agaccggatt tggcggagtc gtggatatgc ttagcgagtt gatggaaact
    52201 gggaaaatca actaccagtt aagaagaaaa ccgcaaaatc ggacaatcat agctgaaatc
    52261 ataggagagc aaaacagtca atctcaattg atggataaaa tcatcatcaa gaaaattttt
    52321 ctacatgagc tataagaaaa ccatgttcgc acttctaggc aagccaggct ttgaagacct
    52381 tgccaaagaa ttgaacgaac gcctttaaga taagggtttt tcgctagatt taaaaaatca
    52441 gtgatagaaa catgttcgct tataaataaa attagtgata gttttagtga tagtgagctt
    52501 aaaaaagcac tcaatttgag tgctttttta tttgctcttc tatatagttt tctagttgat
    52561 tcattgatct ttttttcact tcgggagaaa tatgggcata gacttgtgta gtggaaacat
    52621 ctttatgacc taaaaaatct ttgatatctt ctaaaggtat tcctgcttgt ctcgttctag
    52681 ctgaaaaagc aaaacataat gttggtgttt tcctttcagc atatctatca gcaagatgta
    52741 gagtaggaca acctcgtgag ccaaaagtcc tgtgcaaatc gagttacaac acacacatga
    52801 taaagaaact tttgaaaaga actataaagt atacgttgaa ttggcaagag attcagcaat
    52861 gaaatatggt attccattaa cattagacac tccttataac caaccaggaa tcaaatcgca
    52921 tttatgggta acacaaaata tttggggcga tcatacagat ccttacggtt atctttctga
    52981 aatgggtgta agtaaagaaa aactagccta tgatttggct catggtttta cggatgataa
    53041 tccaactact tcggaggata aaccagtaat tgatccaact cgagcaggtg ctgcaaattc
    53101 tacgctgaca gatggaacaa attacgccca cattgatcag ttcggagaaa tcgaaaacgc
    53161 aaacttgcat gtagctggat ggcacattgc taattataaa tacgagtata ttttcattat
    53221 ggactacaat actggaaaag aactagctag agtaagagct gatgggattt atagaccaga
    53281 cgtaaatcaa gcttataata ctttaggaaa tgttggctat catgtatctt tcaatatgcg
    53341 tgatttccct aataagaaag tatacgtcat gatgcgtgcg acaaacgatc cagaaggaaa
    53401 tactaagggt ggagcacaag attttcatga caagcgttgg tatttaaata ttccacaacg
    53461 ataaaaaatc cccccctctt t
//
LOCUS       DAZAID010000007        53461 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000007 DAZAID010000000
VERSION     DAZAID010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.007"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..908
                     /locus_tag="ACG9BE_000358"
     CDS             51..908
                     /locus_tag="ACG9BE_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFN3031141.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1023..1943
                     /gene="pstC"
                     /locus_tag="ACG9BE_000359"
     CDS             1023..1943
                     /gene="pstC"
                     /locus_tag="ACG9BE_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFN3031142.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1943..2827
                     /gene="pstA"
                     /locus_tag="ACG9BE_000360"
     CDS             1943..2827
                     /gene="pstA"
                     /locus_tag="ACG9BE_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFN3031143.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2842..3648
                     /gene="pstB"
                     /locus_tag="ACG9BE_000361"
     CDS             2842..3648
                     /gene="pstB"
                     /locus_tag="ACG9BE_000361"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFN3031144.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3664..4422
                     /gene="pstB"
                     /locus_tag="ACG9BE_000362"
     CDS             3664..4422
                     /gene="pstB"
                     /locus_tag="ACG9BE_000362"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFN3031145.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4435..5112
                     /gene="phoU"
                     /locus_tag="ACG9BE_000363"
     CDS             4435..5112
                     /gene="phoU"
                     /locus_tag="ACG9BE_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFN3031146.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5689..6381
                     /locus_tag="ACG9BE_000364"
     CDS             5689..6381
                     /locus_tag="ACG9BE_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFN3031147.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6378..7145
                     /locus_tag="ACG9BE_000365"
     CDS             6378..7145
                     /locus_tag="ACG9BE_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFN3031148.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7245..8813
                     /gene="liaX"
                     /locus_tag="ACG9BE_000366"
     CDS             7245..8813
                     /gene="liaX"
                     /locus_tag="ACG9BE_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFN3031149.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9110..9430
                     /locus_tag="ACG9BE_000367"
     CDS             9110..9430
                     /locus_tag="ACG9BE_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFN3031150.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9430..9780
                     /locus_tag="ACG9BE_000368"
     CDS             9430..9780
                     /locus_tag="ACG9BE_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFN3031151.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9947..10885
                     /gene="hprK"
                     /locus_tag="ACG9BE_000369"
     CDS             9947..10885
                     /gene="hprK"
                     /locus_tag="ACG9BE_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFN3031152.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10900..11730
                     /gene="lgt"
                     /locus_tag="ACG9BE_000370"
     CDS             10900..11730
                     /gene="lgt"
                     /locus_tag="ACG9BE_000370"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFN3031153.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11774..12799
                     /locus_tag="ACG9BE_000371"
     CDS             11774..12799
                     /locus_tag="ACG9BE_000371"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFN3031154.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12814..13752
                     /gene="galU"
                     /locus_tag="ACG9BE_000372"
     CDS             12814..13752
                     /gene="galU"
                     /locus_tag="ACG9BE_000372"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFN3031155.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13877..14317
                     /locus_tag="ACG9BE_000373"
     CDS             13877..14317
                     /locus_tag="ACG9BE_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFN3031156.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14320..14922
                     /locus_tag="ACG9BE_000374"
     CDS             14320..14922
                     /locus_tag="ACG9BE_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFN3031157.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14981..16087)
                     /locus_tag="ACG9BE_000375"
     CDS             complement(14981..16087)
                     /locus_tag="ACG9BE_000375"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFN3031158.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16297..17298
                     /gene="ccpA"
                     /locus_tag="ACG9BE_000376"
     CDS             16297..17298
                     /gene="ccpA"
                     /locus_tag="ACG9BE_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFN3031159.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17635..19980)
                     /locus_tag="ACG9BE_000377"
     CDS             complement(17635..19980)
                     /locus_tag="ACG9BE_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFN3031160.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20259..21161
                     /locus_tag="ACG9BE_000378"
     CDS             20259..21161
                     /locus_tag="ACG9BE_000378"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFN3031161.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21374..23149
                     /locus_tag="ACG9BE_000379"
     CDS             21374..23149
                     /locus_tag="ACG9BE_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFN3031162.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23215..24801)
                     /locus_tag="ACG9BE_000380"
     CDS             complement(23215..24801)
                     /locus_tag="ACG9BE_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFN3031163.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            25052..25276
                     /locus_tag="ACG9BE_000381"
     CDS             25052..25276
                     /locus_tag="ACG9BE_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFN3031164.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25417..27168
                     /locus_tag="ACG9BE_000382"
     CDS             25417..27168
                     /locus_tag="ACG9BE_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFN3031165.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            27168..28952
                     /locus_tag="ACG9BE_000383"
     CDS             27168..28952
                     /locus_tag="ACG9BE_000383"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3031166.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            29278..30129
                     /gene="licT"
                     /locus_tag="ACG9BE_000384"
     CDS             29278..30129
                     /gene="licT"
                     /locus_tag="ACG9BE_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFN3031167.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            30225..32105
                     /locus_tag="ACG9BE_000385"
     CDS             30225..32105
                     /locus_tag="ACG9BE_000385"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN3031168.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(32145..33047)
                     /locus_tag="ACG9BE_000386"
     CDS             complement(32145..33047)
                     /locus_tag="ACG9BE_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN3031169.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            33278..34681
                     /locus_tag="ACG9BE_000387"
     CDS             33278..34681
                     /locus_tag="ACG9BE_000387"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFN3031170.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            34681..36114
                     /locus_tag="ACG9BE_000388"
     CDS             34681..36114
                     /locus_tag="ACG9BE_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFN3031171.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            36259..37185
                     /gene="trxB"
                     /locus_tag="ACG9BE_000389"
     CDS             36259..37185
                     /gene="trxB"
                     /locus_tag="ACG9BE_000389"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFN3031172.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            37399..39126
                     /locus_tag="ACG9BE_000390"
     CDS             37399..39126
                     /locus_tag="ACG9BE_000390"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFN3031173.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            39141..39491
                     /locus_tag="ACG9BE_000391"
     CDS             39141..39491
                     /locus_tag="ACG9BE_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFN3031174.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(39583..40443)
                     /locus_tag="ACG9BE_000392"
     CDS             complement(39583..40443)
                     /locus_tag="ACG9BE_000392"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFN3031175.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            40535..40963
                     /locus_tag="ACG9BE_000393"
     CDS             40535..40963
                     /locus_tag="ACG9BE_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN3031176.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            41125..41301
                     /gene="rpsU"
                     /locus_tag="ACG9BE_000394"
     CDS             41125..41301
                     /gene="rpsU"
                     /locus_tag="ACG9BE_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFN3031177.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            41328..41774
                     /locus_tag="ACG9BE_000395"
     CDS             41328..41774
                     /locus_tag="ACG9BE_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFN3031178.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(41928..43307)
                     /gene="brnQ"
                     /locus_tag="ACG9BE_000396"
     CDS             complement(41928..43307)
                     /gene="brnQ"
                     /locus_tag="ACG9BE_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFN3031179.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            44013..44984
                     /locus_tag="ACG9BE_000397"
     CDS             44013..44984
                     /locus_tag="ACG9BE_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFN3031180.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            45008..47200
                     /locus_tag="ACG9BE_000398"
     CDS             45008..47200
                     /locus_tag="ACG9BE_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFN3031181.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            47217..47693
                     /gene="ybeY"
                     /locus_tag="ACG9BE_000399"
     CDS             47217..47693
                     /gene="ybeY"
                     /locus_tag="ACG9BE_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFN3031182.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            47677..48072
                     /locus_tag="ACG9BE_000400"
     CDS             47677..48072
                     /locus_tag="ACG9BE_000400"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN3031183.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            48087..48986
                     /gene="era"
                     /locus_tag="ACG9BE_000401"
     CDS             48087..48986
                     /gene="era"
                     /locus_tag="ACG9BE_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFN3031184.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            49129..49941
                     /gene="recO"
                     /locus_tag="ACG9BE_000402"
     CDS             49129..49941
                     /gene="recO"
                     /locus_tag="ACG9BE_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFN3031185.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            50325..51242
                     /gene="glyQ"
                     /locus_tag="ACG9BE_000403"
     CDS             50325..51242
                     /gene="glyQ"
                     /locus_tag="ACG9BE_000403"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFN3031186.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            51244..53319
                     /gene="glyS"
                     /locus_tag="ACG9BE_000404"
     CDS             51244..53319
                     /gene="glyS"
                     /locus_tag="ACG9BE_000404"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFN3031187.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        17351 a         9482 c        11399 g        15229 t
ORIGIN      
        1 gtaatgaaaa tgataaaata aacatacttt aggattaaag gagatttcta atgaaaaaac
       61 ttaaattatt cttaccaata ataggattag ctattctttt atcaggctgt tcaaagtgga
      121 ttgatcgagg agaatccatt acggctgttg gctcttctgc tttacagcct ttagtagaaa
      181 cagttgctga aacttaccag acagaaaatc ctggaaaatt catcaatgta caagggggcg
      241 gaagcggaac tggtttgtct caagtacagt caggagcagt cgacatcgga aactctgatt
      301 tgtttgcgga agaaaaatcg gggatcgatg cctcagcatt agtcgatcat cgagtagcag
      361 ttgttgggat cactcctatt gtgaacaagg atattggcgt aaaagacatc agtatggaaa
      421 acctgaaaaa actatttaca ggcgagatca aaaactggag tgaagtcggt ggaaaagatc
      481 agccagtagt tatattgaac cgcgcttcag gaagcggaac ccgtagcacg tttgaaaaat
      541 gggtattgga cggagaaaca gctgttcagg cacaagaaca agattcaagc ggaatggtgc
      601 gccagatcgt tgccgacaca cccggtgcaa tcagctatgt tgctttttct tatgtttccg
      661 atgatgtaga tacgttgaat gttaacggtg tagaaccgac agatgaaaat gtgacaacga
      721 acaaatggac gatttgggca tatgagcata tgtacacaag agatcatcca aaaaaactga
      781 caaaagaatt tttagactat atcttatctg atgaagtaca agataacatc gttggagaac
      841 taggttatat ccctgtttct catatgaaag tcgaacgtga ttggcaagga aatatcgtta
      901 aagagtaatc tttacacaaa atttacaaac ttagctaaac ttttacatgt tattaaaacc
      961 aaattgatgt tcaatttgta cactgagtga gtatgttaaa cctgattaag ggggagtcat
     1021 ctgtggaaga tgtgaaaaaa gtattattaa caaaatcaaa acgttcaaaa atggaacagc
     1081 gtggaaaatt catcagtttt ttatgtatcg cgctgatcgt gttggttgta ttatctattt
     1141 tttattttgt agctagtaaa ggattggcca ccttttttac tgataagatc aatgtatttg
     1201 attttctgtt tggtacagat tggaatccaa gccaaatcgg agcaaatggg aaaccaatgg
     1261 taggggcatt gcctatgatc acaggttcgt ttattgtgac ctttttatct gctatcgttg
     1321 ccacgccatt tgccattggt gcagcagtat tcatgacgga gatctctcct aaaaacggaa
     1381 cgaagatctt acagccggtc atcgagctgc ttgtaggtat tccttctgtc gtttatggtt
     1441 ttatcggttt atcagttatc gtgccttttg tcagaagtat ttttggcggg actgggtttg
     1501 gtatcttagc tggtacattt gtgttgttcg ttatgatctt gccgactatc acaacgatga
     1561 ctgttgatgc attaaaggca gtgccacggc actatcggga agcttcatta gcgcttggtg
     1621 ccacaagatg gcaaacaatc tacaaagtcg tgctacgagc agccgtgccc gggattttaa
     1681 cagcagtcgt ttttggtatg gctcgtgcgt ttggtgaagc cttagctatc caaatggtca
     1741 tcggtaatgc agcattgatg ccaacaggtc ttactactcc tgcttctaca ttgacgtcca
     1801 tcttaacaat gggaatcgga aatacaatca tgggaacagt tgaaaataat gtgttatggt
     1861 cattagcgct gatcttgcta ttgatgtcat tattctttaa tatcgtgatc cgtatgattg
     1921 ggaagaaagg agagctgaaa taatgaacgc aaaacgctca gataaaattg ctacaggtgt
     1981 tttatatata gtttcgggga tcatcgtatt gattttagca gctttactac tttatatctt
     2041 agttcgggga ataccgcaca tttcatggtc cttcttgacc gaaccttcca aagcctacca
     2101 agaaggcggt ggaatcggga tccagctatt taactcgatt tatttgcttt tgatcacgat
     2161 gatcatcagt tttcctattt ctttaggtgc gggtatctat ctttcagaat atgctggaaa
     2221 aaactggcta acagatgtga ttcgcacgtc gattgaaatc ttgagctcgt tgccttctgt
     2281 cgttgtcggt ttattcggtt tcttggtttt cgttattcaa ttccagtatg gtttctcgat
     2341 tatttctggt gcattggctt tgactgtatt caacttgcct cttttgacta gaacagttga
     2401 ggagtcttta caagcggtcc attacacaca acgagaagca ggattggcct taggtttgtc
     2461 acgctgggaa acagtgataa aagtggtggt tccagaagcg acaccaggga ttttgactgg
     2521 tgtgatccta agttctggaa gaatttttgg tgaagcagct gcattgatct atacggctgg
     2581 acaaagtgca cctgcgttag attttacgaa ctggaatcca ttaagtgttt caagcccgat
     2641 cagcattttc cgtcaagctg aaacattagc tgtccatatt tggaagatca atacagaagg
     2701 cacgatgcct gatggtgttt ctgtttcggc tggcgcttct gccgtgttga ttattgctgt
     2761 tttactgttc aacttcggcg ctcgtgcgat tggcaaacga ttataccgaa aaatgacttc
     2821 agcctaagga gaagaagcaa aatgaaaaaa tataactgga atgaaacaaa tgtcatcacc
     2881 atgccaaaag agaaagaagt agcactatat acggatgatc tacatgtctg gtatggcgac
     2941 aatgaagcaa tcaaaggagt ggatctggaa tttgaaaaaa ataagatcac tgcactgatt
     3001 ggtccttccg gctgcggaaa atctacgtat cttcgttcgt tgaatcgaat gaatgatggg
     3061 atcgctaata cgaaaatcac tgggaaaatc atgtatcaag gcgtagatat taatgcgcct
     3121 caagtggatg tttatgagat gcgcaaacgg attggcatgg tttttcaacg tcctaatccg
     3181 ttcagcaagt cgatttatga caatattaca tttgctttga aacagcacgg cgaaaaaaac
     3241 aaaaagaaat tagatgaaat cgttgaaaca agcttgaaac aagctgcctt gtgggaccaa
     3301 gtaaaagaca gcttggacaa aagtgccttg gctttatctg gcgggcaaca gcaacgttta
     3361 tgtattgctc gtgcgatcgc gatgaaacct gatatcttgc tgctagacga gccggcaagt
     3421 gctttggacc ctatttcgac aggaacggtt gaagaaacac ttgtaaactt gaaagaagac
     3481 tatacgatca tcatcgtgac gcataacatg caacaagcag ctcgtatcag cgactatact
     3541 gcctttttct atttaggaaa agtaatggaa tatgacaaaa ctaacaagat atttaccaga
     3601 ccaaaaattc aggcaaccga agattatgtg tccggtcatt ttggttaaga cggaggcctc
     3661 aacatgacaa aagagattat ttcatctaaa gatcttcatc tgtattatgg aaagaaagaa
     3721 gcactaaaag ggatcgatct aagcttcaat caaggggaaa tcactgcgat gatcggtcct
     3781 tctggttgcg gaaaatccac ttatctgcgt tctttgaacc ggatgaatga cctgattccg
     3841 aatgtaacga tcaccggaag tgttttatat aaagacaaag acatctatgg tccaaaaaca
     3901 gatgttgttg aactaagaaa agaaatcggc atggtattcc agcagccaaa cccatttcct
     3961 ttttctatct atgaaaatgt catctatgga ttgaaattaa aaggggaaaa agataaaaaa
     4021 gtccttgatc aagtagttga agaaagcctg aaggcagcgt ctgtttggga tgatgtgaaa
     4081 gacaaattgc ataaaagtgc tttatcttta tccggtgggc agcagcagcg tgtatgtatc
     4141 gcacgtgttt tggcagttaa tccggaaatc atcttattgg atgaaccaac aagtgcgctt
     4201 gatcctgttt ctactgggaa aatcgaaagt atgctgttag aattaagaga gcggtatacg
     4261 atgatcatcg tgacccataa tatgtctcaa gcttcacgta tttcagataa gacggcattc
     4321 tttttagatg gtcacttgat cgaatacaat gatacgaaga agatattcat gaatccagaa
     4381 aaacaagaga cagaagatta tatttcaggt cgatttggat aaggaggaaa cggaatgcta
     4441 cgtacacaat ttgaagaaga attgctgaat ctccataatc agttctacga aatggggatg
     4501 atggtgagca gcgccgtgca caagtcagtt cgtgcataca tcaaacacga taaaatctta
     4561 gcccaagaag ttatcgaaaa cgatataaat atcaataaca tggaaacacg cttggagaag
     4621 aaaagttttg aaatgatcgc attgcaacag ccagttacaa ctgatttgcg tatgatcatt
     4681 acggtcatga aagctagctc ggatttagaa cgtatggccg atcatgcggt gtctgttgct
     4741 aaatccacga ttcgcgtaaa agggcagacg agaataccgg aaatcgaaaa agagatctcc
     4801 gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc tagttgctta tgtcaaaaca
     4861 gacgaaaaag acgcacgtac gatcgccaat atggatcaaa gagtcaatga ctatttcaat
     4921 cgaatctata atgcaacaat caaaaatatg caggaaaatc ctgagacagt catcagtgga
     4981 acagactact tgaatgttgc cagttatcta gaacggatcg gagactatgt gacaaacatc
     5041 tgtgaatgga tcgtctactt ggctacaggg aaaatcactg agttaaatac gaaccacaat
     5101 gagattttct aaactgaagc tgtgattatg ccgtttgttt aggaaaagag gttgtgaaat
     5161 cagcggttgt gaaaaagctg ttctgcatca agtaataaga acagtcaaac aaaaaatagc
     5221 tcctcatatt tttcgtttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt
     5281 tattcgggaa agggtttgtg acaaaacatg tcacaaaccc ttttcatcat ttcatagaaa
     5341 ggataaagaa tgcaaaaaaa aattagcatc atcactatat tttttatttg tgtgcttact
     5401 tcatgggtta ttcagcatca agccgcttta tatgaagtac ctgtcatcga gatagaaaaa
     5461 gtacgaataa aagaggatca tcaattgatt acaggaaaac tgatgaacac ggatcaaaaa
     5521 ggagaatcga tttatttatc ggttccttat caaaaaaatc aaatagaaac agcaaatata
     5581 aaagcgagac aaatgtatct agtccaaatg catggagatg aatggcagct agtagcaaaa
     5641 aaaaatgatg gctggctttt cttcttcaca agtatcttca ttggcgccat gttgcttatt
     5701 ggtggaaaac agggtctatt tgctttagga agtctcatca tcaatatcgt tttactttta
     5761 cttttgttgc tcatctttac taaaacagaa gggatttctc tattaaatat atctatcctg
     5821 ttcgtcgtta tagggatgat tattagcata ttggctttag atggatggaa tagaagcagt
     5881 ataatcaaaa tttcagctgc agttacgagt gtttttttag cattttttat ttgtcttttg
     5941 acaatgaatc attggaaaga ccaaggactg cgctatgagg aactgaatta tctgacacga
     6001 ccgtatcgtt ctgtttttct ttctagcgta ctgattggaa cagctggagg aacactcgat
     6061 accgttatca cagtcattgc gacattagaa gaattgacta agcgacaacc agaaataaca
     6121 gcgaaacagt tatggctttc aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc
     6181 aatatccttt tgttcgtcta tatcagtggg tcagttccat cgcttctttt atatttagga
     6241 aatcaatggt cgttcaaaga aacgatagaa cagcatttat cattggaatt tttacgcgtc
     6301 attgctggca gcttaggaat agtattatcg atccctatct cggtcctctt ttttctgact
     6361 gtaaggaggc tgaaacgatg aatgtactta ttatacttgt tgggatttta gcaatcagcg
     6421 tgctacttat tggcggtacg caaggtatgt atattctttt aggcttattc atcaatttag
     6481 ggatcttttt cctgttgtta ttcggttatc atcagaagtg gccgatactg gcattgtcgg
     6541 tcataggatt tttattgatt gctgtggtta tcttattttt tatcaacggt tataatctaa
     6601 aaatgcgtgc agcttttgtg agtatactga tctttttatt ttgtttccta ctattgcttc
     6661 ctatcactga ttttttagca atccaaggtt ttacatcggt agaactagaa gaattatctg
     6721 gcttagataa gactttagcg attgatttta gattacttac gagatcgttg cttctgatta
     6781 gtcttagtgg tgctgtttta gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat
     6841 atcaggcaaa tccgcaatta tctttttacc aattgcgcca ttctagtttc gctattgcta
     6901 aaaaaatatt ggcgtcaaca gtaatgacac ttctttttgc attcatggga agttctttag
     6961 cgttgatcct ctggtttatt gatttagaca ttccttttca gcaagtgatc aatgaaaaat
     7021 catttgttct agaatatacg atggctattc ttaccacatt gtccgcacta cttgttcttc
     7081 ctttaacctg cgtgactaca gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg
     7141 agtaaaagtt acaacttaag tcctatgaca tttttactga atcagtgcat aatatggatg
     7201 taaggaaaag cttgcaaaca attttgctta aggaggcaat ttccatgaaa gaaagagaac
     7261 gtattttaga tttagtgaaa aaaggaatcc tttcgactga agaaggctta gatttattag
     7321 aaagcatggc aactgaaaaa gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg
     7381 ctagtcataa agaaaaagat caggcaagtc aattgatcga taaacttgaa aacggcgaag
     7441 aagaaatttc tgagccagtt gatccaaagg aaagagaaag acaagatcaa gaaaacttag
     7501 aaaagatctt agatgaacta gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag
     7561 taaatgctca attcgctgac aataaggcag cacgcaatga gaaacaggaa gcattgatgc
     7621 agctgaatac aaaagaagaa ttaggcgagt tgacagaaga agaattagca cagcgtcaga
     7681 cgttagaagc agaaatcaaa gaattagaag ctagtggcga tacactgtta gaagaacaat
     7741 tgaaattaga agcagaatta aaagatatcc gtaagaatca atggtctgag aaaaaagaaa
     7801 cgtttactga taagtttgaa ttgccagatg attggaaaga tcaagcaaca gatacactga
     7861 atcaagtagg cgaaaaaatg tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa
     7921 cattccaaac tgtttcagaa acagtgaatg acaacatgga atggaaagat gtcagcttac
     7981 gtgtccctgg gattgcgaca acgaaatttg agcatgaatt ttactatgaa gctcctgcag
     8041 caagtatcct tgatatcaag gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca
     8101 acgatgtgaa agttgaagct aagatcaaac tttacggaaa aatgggtgct gaaccatttg
     8161 aagcattctc tgaacggagc cagattgagg ttaatgaaga tcatatctcc ttccaaattc
     8221 caaataaacg tgtgcgtgca gaccttgttt tctacttacc taaacgagta tacgaccacg
     8281 cagctattaa acttctaaat ggcaacatca tgattgaaac attggaagct aaagatattt
     8341 atacaaaatc tacgaatggt aacatcatcg tggaccaatt aactgcaacg atgctggaag
     8401 tagaaggtgt aaacggcaat atcgatattc gaaacgggaa catcttagat tctattatcg
     8461 aaacagtaaa tggtacagtg acatttggtg cgacagcaga aaacctttct gtgtctttgg
     8521 taaatggtga tgtacgtctt acgattaaag aagataattt gaaaaaagta gaagcaagtt
     8581 cagtgaacgg aaatgtcaaa gtcgctttac cagatggcat cggtttggaa ggacatgcta
     8641 aaacaagtct tggaagtatc aacagccgtc tgtccaatta cgaagtagtt cgtgagaaaa
     8701 aagaacggac aaaccaaatg ttgcaattcc gtcgtgtttc agatggtgaa atcgcgcaag
     8761 tgcaactttc aacgacaaca ggaagtatct atttaaaaga tacagataaa taaagaggtt
     8821 gtgaaagaga tgttcagcct tgagcaataa gaagcgattt tgtaaaatag cttcccctat
     8881 tttacaaaat tgtgccttat tgccgatagt ctatctcttg aacaccgttt accaagagat
     8941 tatgaacctg ccaattatcg ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt
     9001 tttatgaata tggtaatttg tgaccgagaa ttttgcagaa agacaccgat taagaagaag
     9061 gttgtcacaa cgattttctt catttaaaat agtagaacag aggtgagaca tgatgaaaaa
     9121 aaagttgaca aaatctaata aaaatgttgt tttgacaggg actttagcag gaataggaga
     9181 atacatcggg attgatccga ctgttatccg tgtgctttat gtattcctga gcttagttgc
     9241 ttttggaagt ccaattattt tatacatttt attagctttg attatcccat ttgcgcgttc
     9301 tgcacgaaca aactatggac ataacaatgc atattatgaa aaaaacaatt atcgtgaaca
     9361 aactcgtgaa caaacaaaaa aacgcaaaga agcggaaaaa atcgatgatg acgaatggag
     9421 tgacttttaa tgtcgtactt tcaacgtatt ttagttaata cgttaacatt catatcattg
     9481 gctgttcttt ttcctgcgat ggtccatgtg agcagtattt ggacggccgt tcttgccagc
     9541 ttcgtattgt ctgtgttgaa tatgtttgtc aaaccgattt tgacgatact gtccctgccc
     9601 tttacattag tcacattggg attgtttacg tttgttatca atgcagcgat tcttaaaatg
     9661 acctcctttt ttgtgggagc agaacatttt agtttttctt cattcggtgc agcgttgtta
     9721 gtagctatct tgatgtcgct tgtcaatgta attgtcagcg aacataacag tagaaaataa
     9781 aagaaagctg tgccgaacta tcggcgcagt ttttttattt agaatcagga atgcgttttc
     9841 tattacattg gtaattaaat aaaaacgttt tttttaattg gtagtacttt tggctagaca
     9901 atgataaaat gatagagaac actctaatag aaatgaggta gcatgaatgt cagaagttgt
     9961 aaaagttagc caattagtga aaaaattatc tttggaaatc gtgacaggcg acgagaaaag
    10021 cttagaccgt gtcatcgtaa ctggggacat ctcacgccca ggactagagt tgactggtta
    10081 ttttaattat tattcccata atcgtttaca gctatttggc agtaaagaaa tcagttttgc
    10141 ggaacggatg atcccagaag agcgtttgat catcatgaga agattatgta gtgaagatat
    10201 gcccgcattt attatttcaa gaggattacc agctccagaa gaaatgatcc aggcagcaaa
    10261 taaaaatggg atagctgtac tgcgttctcc catctcaact tctcgtttat taggagaatt
    10321 atccagttat ttagatggtc gccttgcccc tcgaacaagt gtccacggtg ttttagtgga
    10381 tgtttacgga ttaggtgtac tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc
    10441 gttggagttg atcaaacgcg gccatcgatt gatcgcagac gatcgagtag atgtttatca
    10501 gcaagatgaa ttaacggtga tcggagaacc gcctaaaatc ctggaacatc tgattgaaat
    10561 ccgaggaatc gggatcattg atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc
    10621 tatgcaagtc cagttggctg tttatctgga agcatgggca aaagataaga aatacgaccg
    10681 tctgggctct gaagacacga ccgttgaaat cgcggaagtg ggcattccac aaataaaaat
    10741 ccctgtcaaa acaggacgaa acgttgcaat catcattgaa gtagccgcga tgaatttccg
    10801 tgcacgtaca atgggctttg acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat
    10861 tgaagaaaac tcaggcaacg actagcaagg agtgacaata tgttagcaca aatcaatcga
    10921 gttgcttttg agcttgtagg gattcctatc tactggtatg cattgatcat cgtatcaggt
    10981 atcattatcg ctatgtggct tagcagcaga gaggctgttc gggtaggatt aaaagcagat
    11041 gatgtgacag attttatgtt gtggggattg cctatctcca ttattggcgc tcgcttatat
    11101 tatgttttat ttgatcttcc ccagtatata gctgatccaa tccaaatatt caatatccgt
    11161 tcaggaggct tagctattta tggcggactg attgcaggcg cgatcacgct gtattttttt
    11221 actaaatatc attttatttc tttgtggact tttttagata tcgctgcacc aagtgtatta
    11281 ttagcacagg caatcggtcg ttggggaaat ttcatgaacc acgaagcata tggaccagac
    11341 acgacgcgaa gcttcttaga aagtctgcat ttaccgcaat tcatcattga taatatgtat
    11401 atcgaaggga tctatcgtca accaacattt ctttatgaat cactttggag tttgattgga
    11461 tttattttgc ttttgttttt acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt
    11521 ttatgttatc taatgtggta tagtttcggg cgtttcttta ttgaaggatt acgtacagac
    11581 agtctttact ttttaggtgt gatccgtatt tctcaattat tatctgctgt gttatttgtc
    11641 ggtacattga ttctatttat ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc
    11701 tcattaggaa aaaatcaaca aattatctga tttttagagc gttaaaaatg aaaaaaattt
    11761 caggagtggt aaaatgaatt atagagtagc ggttttagga ccaggctctt ggggtactgc
    11821 tttggcacaa gtcctcgccg aaaacggaca tgacgttcgg atttggggac atcgtgcttc
    11881 acaagtagaa gaaatcaaca cccaacacac gaatcaacgt tatttaccag atcattatct
    11941 acccgattcc atccaagcat tcaccaatat ggaagatgca gtaaaagacg cagatgcggt
    12001 gctattcgtc attccaacga aagcaatacg ttctgttgca aaagaactag tgcctaaatt
    12061 gaatacaaag ccagtgatca tccatgcaag taaaggatta gaacagggaa cacataaaag
    12121 gatctctgac atcttgttag aggaaatccc agcagaaaaa agaaaagctg ttgttgttct
    12181 ttctggacca agccatgcag aagaagtagc aaaacatgac atcactacga tcacagctgc
    12241 aagtacagat gcaaaagcag caaaatatgt acaagagctg ttcatgaata attattttcg
    12301 gatctatacc aatcctgatg tgatcggtgt agagatgggg gcagctttga aaaatattat
    12361 tgctattggt gctggcgcta ttcacggctt agggtttggc gataatgcaa aggcagcaat
    12421 catgacgcgc ggattagctg agatcagccg tttaggtgta gcaatgggtg ccaacccgct
    12481 gacctttatc ggattaagcg gaataggcga tctagtcgtt acttgcacaa gtgttcattc
    12541 taggaactgg cgcgcaggca acttgttagg acaagggcag cccctagaag aggtgctagc
    12601 caatatggga atggtcgttg aaggtgtgaa tactacccaa gcagcgatgg aactagccca
    12661 aatgatggaa atcgaaatgc ccatcactca aacaatctat gaagtgctgt atgaacaaaa
    12721 agaaatcaaa acagccgcaa aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt
    12781 ctcattaaca attttttgaa ggagcgaatg tatatgaaag ttaaaaaagc agttattcca
    12841 gcagcaggat taggaacaag atttttacca gcaacaaaag ctatggcaaa ggaaatgttg
    12901 ccaattgttg ataaaccaac aatccagttc atcgttgaag aagcgttgaa atcaggtatc
    12961 gaagacatct tgatcgttac aggaaaagct aaacgaccaa tcgaagatca ctttgattca
    13021 aatattgaat tagaaacaaa cctaagtgaa aaaggaaaga ccgaattatt gaaattagta
    13081 gaagaaacga cagatgtcaa cttgcatttc atccgtcaat ctcatccaaa gggtttagga
    13141 catgccgttc ttcaagcaag agcctttatt ggaaatgaac catttgtcgt tatgctaggc
    13201 gatgatataa tggaagacga agtaccattg acacagcaat taatcgatga ttacgaacgt
    13261 actcatgctt ctacgatcgc cgttatgcaa gttcctcatg atgaaacttc aaaatacgga
    13321 attatcaatc caggagaagt attggaagat gggttataca atgtcaaaaa ctttgttgaa
    13381 aaaccagatc caagcaaagc accaagtgat ttagctatta ttggacgtta cttattgact
    13441 ccagaaattt ttgatgtatt gtccgagcaa caaccaggag ccggaaatga aatccaacta
    13501 actgacgcaa tcgatacatt gaataaaaca caacgcgtat ttgcccgtaa attcacaggt
    13561 aaacgttacg atgtcggtga caaatttggt tttatgaaaa caagtattga atatggtcta
    13621 acacatccag aagtaggtgc acctttacgt gagtacatcc tgtctttagg taaaacatta
    13681 gaagcagaag gaagcaagat cgatcataat ccaaaaggaa taacagaaat caaagaagaa
    13741 aagaaaaaat aacccttttt tctaacgagc ttcagctgct aattgaataa aaagtagttg
    13801 aagctcgttt ttatttgttt aaaaaacacg aaaatgctat ccttatagaa aagagagaca
    13861 agaagggagt ttgatcatgt caggaggaga aattgcagga ttgatcgcag caatcgcctt
    13921 tgcgatactt gtcgttttta tcgttcgtgt gttgttgcaa gtttctaaaa ctttagaaaa
    13981 agtagatcag actgtagcag aagcaaatac gacgatcgaa atcgtcacaa aagacgtaga
    14041 tcttttgtct agacaagtag aaggcttact ggtcaaaagt aatgaactgc tcttggatat
    14101 caatggaaaa gtcgcaacga tcgatccatt gttcacagca gttgctgatt taagtgaaag
    14161 tgtatcagaa cttaatcatt ctagtaaaaa catcgcgacg aaagtcggcg gaatcggtaa
    14221 aacgacagct aaagcgacgg ttgccggaaa agtcggtggg acagcgatga aattttttaa
    14281 aaacaaaaaa catactgaaa cgacaggagg aaaataaaaa tggctaaaaa aggtggattt
    14341 ttattaggtg cagtagtcgg aggcacagcc gcagcaatcg ccgcgttatt gttagcacca
    14401 aaatctggta aagaacttcg ggatgaatta gctgcccaag cggatgattt gatggatatg
    14461 gcttcagatt acacagatat ggcgaaagaa aaatcgactg agttaacagg tattgcaaaa
    14521 gaaaaagcgc aagatctttc tcaacaagca ggtgatctag caggtagtgt caaacaaaaa
    14581 gtaagtgaat cagcagatga agcaggaagt gaatctgacg atatcttgtt aaatcttaaa
    14641 gaacaatcag ctgacttatc taatcgtttc aaaaaaacgg cagaagatat gaaaaaacct
    14701 gcagatgaat taggaagtat tgctaaagat acttcagaag atatcatgat tgatgtaaaa
    14761 gacgctgctg gtaaagcagg agatactgtc aatgaaggaa tgtcagaagc aaaaccagtt
    14821 gcagatgact caaaagaaat gatgaaaatg gcaaaagaag atgcgaaaga agctaaaaaa
    14881 gaaataaaag atacaatgac agattcatct acaaatcact aaaatcacaa atttatagaa
    14941 taaaaaggtg ggagcggact agctctcacc ttttttactc ttatttttct aaaacaagta
    15001 attcttttgg tgtagtcgtg aatggcacac agccatcgct tgtcacatat acacagtctt
    15061 cgatacgtac ccctactttt tcaggaatat agattcctgg ttcaagagag aagcacatcc
    15121 cttcttcgat tactaagtca tttccttcta caagggaagg gaattcgtgg acggttgttc
    15181 cgatcccgtg acctaaacgg tgattgaaat attcgccgta gccatagctg ttgatcacgt
    15241 ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg acggacggct tcttgtgcgg
    15301 caagctgtgc ttcaagagtg atgttgtaga ttttttcttg gaagtcggtt ggtttttgat
    15361 aagcaattgt acgagtagca tcactgcaat agccattcca aacaacgcca agatcgaata
    15421 acacaagttc attcggtgaa atcttcgtat ttcctggtgt accatgaggg cttgcagcat
    15481 ttgctcctgc aaggactaat gtgtcgaaag acatttgtga aacaccttgt tttttcaatt
    15541 ggtattcaat ttctgcaatg atcgcttgtt cagtcacgcc ttctttcact gctttaaagc
    15601 caatctcaaa tgcaacatcg gcccatttac cagcttcgat cagtttgtct ttttcctcta
    15661 gagttttgat cagttgtaga cgttggatga tggaagttag atcagtgtaa gaatccgttc
    15721 ccgtcaagat atcagagata gcatcgaagc gctcgacagt caaggcgttc ttttcaatcc
    15781 ctacattgcc aggctgacca tagcgagcac gaagttcttt tccgatgatc tcccaagggt
    15841 tttcgctgtc taaatagcca cggacatcat acgcccaact actgttttga gcatcttcaa
    15901 cttccaaagc aggagtgaaa aggaaaggat cttgttcaag agggataaaa agagctagta
    15961 cacgctcatg aggatcactt tcgtatccgg aaaagtaagc gatgtggcca gggtcgctga
    16021 tataagccaa ttcgacttgg tgttgtgcca tccattgacg tagttcattg atttttacgt
    16081 tattcatttg gcaacctact ttctaaatta attttgtatt caataattgt accactgatt
    16141 tattaaaaga ccaacgttct aaaaactgaa aaaagtaaaa attttcatga aaacagagat
    16201 aaaacggttg caattcttga gattatttga tattctattt tagaaatgta agacgtattt
    16261 tcagaaaaca tataaaatat gaataggaga acacaaatgg aaaagcaaac aatcactatc
    16321 tatgatgtag cgagagaggc aaatgtttcg atggctactg tttcacgtgt cgtaaatggc
    16381 aatccaaacg ttaaaccagc aacaagaaaa aaagtcttgg aagttatcga tcgcttggat
    16441 tatcgtccaa acgcagttgc gcgtggcttg gcaagtaaga aaacaacaac agttggtgta
    16501 atcatccctg atgtcagcaa tatgttcttt gcttctttag cacgtggtat cgatgatgtg
    16561 gcaacaatgt acaaatacaa tattatctta gcaaattcag atggcaacga ccaaaaagaa
    16621 gtcaatgtat tgaataactt actggctaag caagtggacg gcgtgatctt catgggtcac
    16681 catatcacag acgaaatccg tggagaattt tcacgttcaa aaactcctgt tgtattagct
    16741 ggctctattg atccagatga acaagtaggt agcgtaaaca ttgattatgt atccgcaaca
    16801 aaagatgcag taaacaaatt ggcaaaaagc ggcaataaaa aaatcgcttt tgtcagtggt
    16861 gcgttgatcg atcccatcaa tggacaaaat cgtctgaaag gctataaaga agcattaagc
    16921 gaaaataacc taccatatag cgaaggattg atctttgaag cacaatacaa cttcaaagat
    16981 ggtctatctc tagtcgatcg gatccataat agcggtgcaa cagctgtcta tgtttcagat
    17041 gatgaattag ccatcggtat tttggatggt ttattagacc gtggcgtgaa agtaccggaa
    17101 gaattcgaaa tcatcacaag taacaattca ctactgacag aagtagctcg tcctcgttta
    17161 acaagtatca cacaaccatt atacgattta ggggcggtat ctatgcgcct attgacgaaa
    17221 atgatgaata aagaagaagt cgaagaaaaa acgattatct tgccttatgg aatcgaagaa
    17281 aaaggatcta caaaataata aaaggacaac cattcagagg ttgtgtgcct gccgtttaac
    17341 tctgcgttac aaggagcaat ttcaaaaaac agcatctcat gtttgatgaa tattgtgact
    17401 cgtgattcgg agttggcagg agaacaccgt ttatttggga aaaggggtta tgacaaaatt
    17461 ttgttataat cctttttttg tgtcttaaaa aagagagaaa gtggttgaaa ttacatttat
    17521 cgtatataaa acaatccgtg tatagatgct gcattttgtt caccaccatc aaaaaaataa
    17581 gggactgtga caatactatt gtcacaatcc cttattactg tgtattctat taaatcaatc
    17641 atctttctca tctgattctt tagttgcttg attcgctctt gcataagctg aagcggtgtt
    17701 ccagtaatct gtatagtttt catcggtacc gccaacgcca aatttaaagg cgcttttctt
    17761 cggtccattt ttcgcccata gcgattcaac tgtttttcct ggtgtttgga tggatttttt
    17821 attgacagta acttttccag gtgtttgacc agtaaatcca gatacttttt tcttcatcac
    17881 atcagatgat aattcgaatt tctcatcagt gccaaaaatg tcaggttttg cttggtaagc
    17941 ttgattgatc agattggcaa gataattagc cgaacgtatc cttgctttgc tgtccattcc
    18001 agtattgtca tcgtgccctg cccagctact gatggtaata gacggagtag agacaattag
    18061 ccatgaatct cggtattcat tggtagaacc agtttttccg acccaatctg cttttcctag
    18121 atttccgttt aagcttgaga tggcatcttt gaatggtgta gtgatttttg cattgatcac
    18181 actacgcatc atgtcattca tgatcgatgc cgttgcttcc gaatagatcc ggacagagtt
    18241 gctttcgtgt ttataaatta catttcctgc gttatctgtg atggaatcga tgatatagcc
    18301 ttcttcatag acgccgccgt ttgctaaagc ctgaaatccg ttggtctgtt gaagcgtcgt
    18361 gacatctact gtacctaaag gagcagattc aacgccccaa ttgtcatttg ccgggtagtt
    18421 catctttgcc aagtagtgct cgtaagcata ttgtttcgat ccaccggaat tcaacacatc
    18481 ttgatataag tgataagccg ggatattgtt ggaccattct agtgactcac ggacggtttg
    18541 gaaggtgttc gatccttcgt tagtagcatt gacgattttc tcgccagcat tttctccttc
    18601 ttgccaagtt gttgcataat ctgatacacg ggattctgat ccgataagcc ccatgtcgat
    18661 tgcaggtcca taaacaagaa ctggtttgat tgatgagcca gcttgacgtt ctgtatcgaa
    18721 ggcatggtta ttttgattct gactgtaatt ccgtccgcca acgaatccgt agatgcggcc
    18781 agttctgtta tccatcaata catttccggt ttctacttga ctgccagcac catcatctaa
    18841 catgtatcca taattttcaa cgccattttg caatgcagca tagacatctt tatcgattgt
    18901 cgaatggatt gtatatcctt tattttggat cgtttgctgt gccttttgat aataagcgtc
    18961 actgaccgat gaatcagaca tatcagcgtt gtctttttca gccaattgtt gtgcaatgat
    19021 tcttgttgct tcattcatca cagtataata caagaattct ctgtcagtct gttctgcgat
    19081 ttgttgtggt agaaagtctt ttgtaaggtc ataggctttt gcttcttcgt attgttcttt
    19141 cgtaatttgg ccttcacgat acatactgaa taaaacaaaa tcttttctgg caaggccggc
    19201 agaaagatct tcttttagcg cgcctgtgtt cgtgtatgga ctatacgtga tcgggctttg
    19261 gggtaatcca gcgatataag cagcttgcgg cagcgtcaca tcttttgcat ctacaccaaa
    19321 gattcctttt gcagcttcct ggactccagc aatgttttgt cccttattat tacgaccaaa
    19381 tggtgagaca ttcaaatacg tagcaatgat ctcatctttt gaaaagtatt tttctacttg
    19441 tgcagaaaga atgatctcgt ttgctttacg tttgaatgtt gtttcatctg taaggatctg
    19501 ctgtttgact aattgctgtg tcaaagttga tccaccagaa gagccaatac cagtagcttc
    19561 agatactaat gctcgtaaaa cagctttagg aacgtaacct tggtgtttat cgaagtattc
    19621 atcctctgtg ctgataattg cagttttcaa taaaggtgaa atcttatcag atgaaatagt
    19681 cgtgcgcatc agatctgtct ggatttttga tatctctgta ttatctgcat aaacaatctt
    19741 tgacgtttct gtaatgtttc ctagttctgt ctgcagtgct tttttagtcg gcaattgtgt
    19801 gtcttcgacg agataggcaa aatagccagc gcctattcca atacctagag ctgcgaacat
    19861 caaaagaaga acagtaataa aaacagtcaa tgattgaaaa actctaaaaa taatcttagg
    19921 aacaaaccat ttttcttttt ttcttctatt ctgttttttt cgagtatttt tgcgtggcaa
    19981 aagggtcacc tcaactaatg gttttatttc gctaattata gcaaataata gaacaaaaaa
    20041 acagacaagg ctgtttttgg caggaatttc ttttttaaaa gagttaaaac gtgagaaaat
    20101 gatctttatt ataaaagagt tcttaagctt ttctttagag aaaatttcag ttactatgga
    20161 gaacagggca aagaaaaatc aatcattttc tttgtatatc atcttaccaa tatataggaa
    20221 ttgttagtaa caattgcctt tcaaggagga atccatccat gttattaggc tcacacgtca
    20281 gcatgagcgg aaagaaaatg ctgttaggag cagcagcaga agcagccagt tatggatctt
    20341 cgacttttat gatctataca ggcgcccctc aaaacactag aagaaaagcc atcgaagaga
    20401 tgaatattga tgccggaaaa gcgtacatgg cagaaaacgg tctaacaaat atcgtcgttc
    20461 atgcccctta tatcattaat ttggggaata cgatcaaacc agaaatgttt ccttttgccg
    20521 tccaatttct gagagaagag attgttcgtg cggaagcttt aggcgctact caaattacac
    20581 ttcacccagg tgctcatgta ggggcaggaa cagaagccgg aattgctaga attattgaag
    20641 gcttgaatga agttttgaca aaagaacaga cggcacaaat cgctttggag acaatggcag
    20701 gtaaaggtac ggaaatcggt cgaacatttg aggaattagc gcaaatcatc gatggagtaa
    20761 aattaaatga aaaattatcc gttacttttg acacctgtca tacgaatgat gccggttata
    20821 atattaaaga agacttttca ggtgtaatgg aagagtttga taagatcatc gggttagatc
    20881 ggcttaaagt actccatatc aatgattcta aaaatccgca aggttcgcat aaagaccggc
    20941 acgccaatat tggttttgga acaatcggat tcgacacgct gaataagatc gtccatgatc
    21001 caatgtttga caatatttcg aaaatcttgg aaactcccta tgtcggcaaa gataaaaaga
    21061 aagcttacgc accatatgga aaagaaatcg ccatgttcaa agcacaaata ttcgatccag
    21121 aagtgtttcc tgagttatta gcagaagaag tgatactcta agattttcta tctgacagca
    21181 gcaaaaaagg accaggcaca ttgccttgtc cttttttgtt taagaaacaa aggagtactt
    21241 tttgcagtct ttttcatcat gcatcttctc ccccctcttt ttaaatttag acaaacaagg
    21301 tatactgtta gaaaagtaaa aaaacgaaaa gtttcacaag caagtggcaa aacgtgattg
    21361 gaaggacgaa aagatgctga acactttaaa acccttgatc aaccaactaa aaaagaaaca
    21421 tacatacttt attcttggca tagccctatt atttacaatt attcgtttgt atttatatag
    21481 taaagcaatc tatggaacag acatgtcggc aacctatgat gaccagctgc tgattcgcta
    21541 tgctcaaaat attttggacg gtaaatggct aggggagtat acgactacaa cactttccaa
    21601 aggaatctcc tacagcttat ttttagtact tgcacataaa ctgcatcttt cttatgctgt
    21661 tttgttcggc atgttcagta tttttgccag tatattggtt gcactcgcgt ttaaaccgat
    21721 cgtgaagaac aagtatcttc ttttaagtat ctatttttat tttttgtttt ctccgattag
    21781 ttttacacat gaatattcca ctcgaacata tcgtaatgcc ctcgtgattc cggctgttga
    21841 gatcattatc gcttgtttac tagctattta ttaccgcaaa tacggttcta gcagaaaatt
    21901 gctgccatgg tttttagggc tttctgtcgc ttttcctttc ttttggttca ttcgcgaaga
    21961 ttctatctgg cttcttcctt ttactgtagt agctttgggt atcagtttcg tccagatctt
    22021 gctagggcaa gacttttctt tcaaagaagt aaagaagtta aaaaaacgac tctttcttct
    22081 ctcgaaacgg gaatggctga aactttcatt gtttttactt cctatagttg gcttttccat
    22141 cgtctatcag tggatcgaat ataaaaatga gacaacatac ggtattgcaa ccatcaatga
    22201 ccgttccagt ggagaatttg gacgtttgat gaaacaactg atccgaatag atgatgaaac
    22261 gaacttgaat aaagaaaaca gtgagatatg ggtctcacat gctgcactaa ataaagcatt
    22321 agccgcatct cctacattcc atactgtttc tacccgtatt gacgaattat atacgactca
    22381 cgcgtggact caaggtggaa aagtaaaaga attacccgga gatatcatct tttgggcatt
    22441 gcgagatgtg gtaagcgaat ctggttatta ccaaaacaat gcaaaagaaa ccaatgattt
    22501 ttggaaaaaa gtcaaccaag aattagaaga agcctatcac aagggaacca tcaaaaagaa
    22561 aaaagagttc tatctgatgg gatcaggaga tggaaagacg atgagtgatc tgccaatcgt
    22621 attcgatttc cttcaatcag gatacgctta tgcatggcga tataaagatt tccatcaagg
    22681 aggaaattat agtttcggaa cgcaagaaca agtcaaacag gctcaagaat tattaaacct
    22741 tccgctaatg gagaattgga tcgatagtaa tgaaccgaga acacctaatt tccaattaac
    22801 aaaatcagca aaagtcgcga atgtcatcat aaaaatttac caagttctgg ctattcccgt
    22861 gctgattgct tcaatagcag ggatattact tatgatacta ggaaccgtat ttgcaaaagc
    22921 tgatcgtgcc tattatagtt cctctttgat tgtggtcacc ggattaatac tgacacaatt
    22981 ggtttttctt ttcggtgtct cgtggttttg ttcttatgcg ccagaaaaaa aggattattt
    23041 tttaagtgtc tacactggcg caggcgtgcc attaacgcaa ttcatcgtag taacaagcat
    23101 gctagttttc agtaaattaa atctgaaaaa ggctttatct tctaaataaa aagacccttg
    23161 gtagctagat ttttaaccta actatcaagg gtctttgtgt ggaaaaatca gtttctattc
    23221 gatttcttct accgtttcct gcaaacgctg ttcatatttt ttgtttcctt tatataaatc
    23281 accaatcgtc catgccaaca ggagaaggac aagaacaatg attacataag cgatgtgatc
    23341 agccaatgct agttgagcag aagtggcttg ttcaccaaag aaactttcga tctgtcctgg
    23401 taatcctcgt aagttcaagt aagttagact aatcacagag aaccagccaa gaagttttac
    23461 gatgaatgaa tttttaaatc ggctacccat ctccgcttgg ctgttggtca tcatcagtaa
    23521 agggatcatc gaaaacggca acgcaaaagc caagaacacc tgggattcat tcatcaagtt
    23581 attcaaagcg gtatgttctt cgatcgttcc ttttgtactt gtaaacaaaa cacaaatcaa
    23641 tacaggaatc acagaaagca atcgtgtgac cagtcttctt agccaaatcg ggatacgcat
    23701 atggatgaag ccttccatga tgacttgacc ggtcaacgta ccagtaatag tagagttctg
    23761 gccagaagca agaagtgcta cagcaaataa agtggataag ataccagatt ttgctacagc
    23821 aatcaagata ccattgctca tagctgatgg atcagataag gcttcataca gaccaaaaaa
    23881 ggaaggatct tggacagaac cggttttgaa gacagcgaca cccatgatca atagcagtgc
    23941 attgactaga aaagccatcg acaattggat attagagtcc caagtagaaa aacgtacagc
    24001 ttgtgctaca tcttcttcgt cttggtgatc aatcttccga gtttgagaaa ctgctgaatg
    24061 aagatataag ttatgcggca tgactgttgc accaataatc cctaaagctc cagtcagcgg
    24121 agtttcgccg ccgatgaccg gtgagtctga gaatgtatga ctgttaggga taaagccagc
    24181 aaataaactg tgccaatctg ggttagacaa gccaacttga tagacaaaaa cgaataaaat
    24241 gacgaaaatt aagcagacaa ccagtgcttc gatcttacga aacccgactt ttgtcagtag
    24301 taagagaacg aaaacatcta atacagtaat aaagacggca atcaccaatg gaatatcaaa
    24361 aagtaagtag agggcgattg ctgcaccaat gacttctgcg atgtctgttg ccataatggc
    24421 taattcagtc aagatccata aaatgacacc caatgtctta cttgtgcgtg ctcggattgc
    24481 ttgggccaaa tccatttggg tcacgattcc taattttgct gccatatact gtaaaagcat
    24541 agcaatcaga ctagacatca aaataactga catcaataaa tattggaaat tttgtccacc
    24601 agtgatcgaa gtggaccagt tacctggatc catataacca actgctacta atgcacctgg
    24661 tccagagtaa gcaagaagtg ttttaaaaaa acttttcccc tttggtacat caactgttcc
    24721 atttacttca gctaaagata aaccatttgt atgttcaata agtcgatgct tatgaaattg
    24781 tttgttggat tgattattca agaaaaaacc acctttccta tttatctacg attacattct
    24841 acacctcaaa aagaaaaaat taaagaaaaa tattcggtat gcctaattta ttttcgtggc
    24901 gcgtcttgtt agatgacgaa agaaattttg ttctcctcta aaaaaaccac tattttttgc
    24961 atgaaactaa agaatacgcc tttctttttt caataaactt tagtaaaata agtcattgag
    25021 agcaattcgg aaatagaaag gagagattta tatgttatca acaggtattg tcgtacttat
    25081 tgctattatt gctttgctaa tcggcgcagt aggtggcttt ttcctagcgc gtaaatatat
    25141 gaaagactat ttagaaaaaa atcctcccgt taatgaggac atgttgcgat caatgatgat
    25201 gtcaatggga caaaaaccat ctgagaagaa aattcgccag atgatgcagc aaatgaagaa
    25261 ccaagggaaa aaataggttc tgacccaacg actttttacg ggctgatttt ttcagtccgt
    25321 tttttatttt gtaatttgtt agactattca gattattcag caaaaagaca agcacagaca
    25381 atcctaggct cttgataaag acaaaggagg agtaaaatgt cgatttttag aaagttaggc
    25441 tggttttttc gtcaggaaaa gaaacattat ttaattgggg ttttctcgct attcgcagtg
    25501 gccttggtac agttagttcc tccaaaagtg attgggatca tcattgatga gattgccgat
    25561 aaaaatttat cagtaaagat cattttcggg tggatcttcc tattgattat cgctgctttc
    25621 ttacaatatc tttttcgtta tatctggcgg acgaatattt ggggaagtgc agctcggttg
    25681 gagaaagaac ttcggcagca gttattcaaa cattttacaa aaatggacaa tctcttttac
    25741 caaaaatatc ggactgggga tttgatggct catgcaacga atgatttgaa tgcgatccag
    25801 aatgtagcag gagctggtat cttgaccttt gcagattcag tgattacggg cggaacaacg
    25861 atcattgtga tgattttgtt tattgattgg cgcttaacac tgattgcgct gattcctctg
    25921 cctttgctgg ccgtcacttc acgagtgctg gggtcgaaac tccatgatgc ttttcgtgat
    25981 tcgcaagaag ctttttctag tatcaatgat aagacacaag aaagtatcac tgggatcaaa
    26041 gtcattaaaa catttggaca agaaaaagaa gatctagcgg attttgaaga aaagattgaa
    26101 gatgcaatta ttaaaaacaa acgagtcaat tttctagact ccttatttga tccattcatt
    26161 accttgatta tcggtctgtc ttatgttgca acaattattt taggtggaaa gtatgtcttg
    26221 gaaggcacga tctcgatcgg gcagatggtc tcatttatta gttatatcgg tatgcttgtt
    26281 tggccgatgt ttgcgattgg tcgattgttc aacgtattgg aaagaggaaa tgcttcttat
    26341 gatcgcgtca atgaactttt gcatgaaaaa acgcatatcg tggaacaact gggtgcagtg
    26401 cagaccccgg taaaaggcga gctgaatgta gctatttcct cttttaatta tccgaaagaa
    26461 gaatcaccag cattagaaca aatcaagttt gcaatccaag aaggagaaac attaggtatt
    26521 gttgggaaaa ctggtgcggg aaaaacaacg atcatcaaat tactgatgcg tgaatacgat
    26581 gattatcaag ggaaaatcca atttggcgga cgttcgatca aagaatattc attgaatgct
    26641 ttattaggat caatcggcta tgtgcctcag gatcacttct tgttttcgat gaccattcga
    26701 gataatatcc gtttttctaa tccaaaactt tcagaagaag aagtagagca tgcggcttct
    26761 ttagcattta tcaacaatga aatcaaggaa ttaccaaaag gttatgatac cttagttgga
    26821 gaacgtggag tctctttatc tggcggacag aaacaaagga tttcgattgc tcgagcattg
    26881 atcaaaaatc ctgaattatt aatattagat gatgcacttt ctgcagtaga tgccaaaaca
    26941 gaagaagcta tcttgcataa tttaaaagaa ataagaaaag agaaaacaac tatcattgca
    27001 gctcaccgtt taagtagcgt gatgcatgca aaagaaatca ttgttgtcga tcaagggaaa
    27061 atcattgaac gaggaaccca tcatgagctg cttgcactag gcggctggta tgaacaaatg
    27121 tgggagaaac agcaattaga agcgaagata gaaggaggcg aggcctgatg gatgaaaatt
    27181 atcaatcgga atggacaaaa tcgattccac ttaaagaaca gaaaaagatc atcaaacgac
    27241 tgatgaagtt cgccaaacct tttagaacaa cgttcatcgt tgctatctta tttgcgtttg
    27301 ctctgtctgt gatcaatgtc ctattgccaa ggatcatcca aacttttatg gatgatcatc
    27361 tggctaaaca gtcggctacg acacaagtta ttttgttttt tgctggtatg tacttatttg
    27421 gagtcctagt aaaaagtatc atttggtttt tccaatggta tttgtattcc atggcttcat
    27481 tgaagaccta tcagtatatt agggtaaaat tatttgaaaa gctgcataca ctaggtatgc
    27541 gttattttga tcagacaccg gcaggatcaa ccgtttctcg agtgacgaat gacacagaaa
    27601 cgctatttga attttggtac gtctttttga tggtattgac tgggatcttt gcagtcgttt
    27661 cttcattcat tgccatgttt caaatcaatg gagaaattgc cttgtggact ttggtgttct
    27721 tgccgatttt aggaattgtc atctggtatt accagaaatt tagttctcgt atttaccgaa
    27781 atatgcggga aaaattaagt cagctgaata caaaattgaa tgaatcgatt tccggcatgc
    27841 gtattatcca gcagtttcgc caagaagctc gtttagctaa agaatttgaa caagtgaaca
    27901 atgagtattt agacacgcgc tatgcgatga tcaaaacgaa ttcgattctg ttaagtccga
    27961 tcatcaattt tctatatgca ttggcgattg cgttggcgtt gactttattt gggatcgatg
    28021 cgttgcattc tcccgtagaa gttggactga tctatgcatt tacgacttat gtccagggat
    28081 tttttaatcc aatgtctcaa atgatggact ttttgagcat cttcacagat gggatggttg
    28141 ctggtagtcg tatcttgaag attatggata cggaagaata tgcaccacaa caaaaccctg
    28201 gagcagatca aatgattact tcaggtaaga ttgagtttcg caatgtaagc ttttcttatg
    28261 atggagagca caatgtatta aacaatatct cttttgttgc taatccggga gaaaccgttg
    28321 cattagtggg acacaccgga agtgggaaaa gctcgatcat caatgtgttg atgcggtttt
    28381 acgaatttta tgaaggtcag atattgattg atgatcatga cattcgagaa tatccaatgg
    28441 aagagttacg taagaaaatg gggctggtct tgcaagatgc tttcttgttt tatggggata
    28501 tcgcaggaaa tattcggatg atgaatccag ctatcacaga cgaacagata cgccatgcgg
    28561 ctgagtttgt tcaagcaaac aaattcatcg atgaattacc aaaaggatat cactcaaaag
    28621 tgatcgaaca tggtgccagc tattctagtg gacaacgaca gctgatcact tttgcgcgaa
    28681 caatcgtgac tgatcctaaa attctagtat tggatgaagc aacagcaaat atcgatactg
    28741 aaacagaagg attgatccaa gaaggattgg cgaaaatgcg acaaggacgg acaacgattg
    28801 cgattgccca tcggctgtca acgattcgag atgcagaatt gattttagtt ttagacaaag
    28861 gtcatatagt agaacgcggg aatcatgaaa cacttctagc gcaaaatggt ttgtatgcag
    28921 atatgtacaa actgcaaagt agtggtatat aaaagatgtt gtgacagtag cattttgacc
    28981 taagcactag agtgaaaata aaacatagct tcccatattt ttcatctgtg agagttagtg
    29041 tcgtagttac cagatttaaa aaagacgacg tcagctagtc agaagtgcct aatagcccct
    29101 aagaaagcgt tgacaaaatc gtttgctttc gataatataa agaaaacgat acaacttgga
    29161 tagtgattgc ctgatgcaag ctaaacctga tgagtctccc atggtttcat ggacggcttt
    29221 ttgggttttt ctttttgttt tggatacagc cctatatatc atggaaagga gcaaaacatg
    29281 ctgattgaga aagtactgaa taacaacgtc gtaataacaa gggatgaaag agatcgggaa
    29341 atgattgtga tgggaaaagg attggcattc aaaaagaagg tgggtgaccc gattcttgac
    29401 tcatgcatcg acaaaaaatt tcagttgaca aatgacggac tttcacaaca gttccaagaa
    29461 ttggcacaag cgattcctct tgagtactta gaaatttctt gtgaaatcat tgaatataca
    29521 aaagaggctc tgcaagtcca tttaaatgat tctgtgtata tttcattgac ggatcacttg
    29581 tatacagcta tcgaacgtca aaaaatgggg atctttgtgc caaatgtatt gctgtatgat
    29641 atcaaacaac tgtttcctaa agagtatgcc attgggaaaa aaacagtgaa gaaaatctcg
    29701 gataaatacc acatcaagtt atcagaagac gaagccggat ttatcgcatt gcatttagtc
    29761 aatgcacaag gcattcaaag ccagcaagac aatacctacc tgtttaccac aacaatacag
    29821 gatattttaa atatcgtccg ctattactat aagattacat ttgatgaaga ttcagtccac
    29881 ttttttcgat tcgtcacaca cctgcgtttt tttctgacac gtgtcctaga tggagcgaaa
    29941 tcagaagatg gagaagtttc tgaggaattg ttccaagtta ttcaaaaaaa atataaggat
    30001 acgtttggtt gcgttgagaa aatagcagac tttttaaccg gggctttcga ctaccagatg
    30061 tccacggatg aaaaaatgta tttaacgatc catactgcgc gtttagtgca tgagaatcgt
    30121 tgtaaataaa taaaaaagga ggacaaatta acaagaacgt atgaaataag ggtagtgaca
    30181 ttcagttggc taaaccttcc actaattaat tggagggaat agaaatggga aaataccagg
    30241 aattagcaga aaacatcgta aagaatatcg gaggaaaaga aaatatccgt agcttgacac
    30301 actgtgtgac gcgacttcgt ttcaaactaa aggaggaaaa taaagcaaag gatgatgttt
    30361 tgaaaaagat ggaagggata gtcaccatca tgaaaagcgg cgggcaatat caagttgtaa
    30421 tcgggaatca tgtgccagat gtctatgcag aagtactgac agtagccggg atttccgatg
    30481 aagcagaaga agaggtttct tcaagtagta atctgttcaa tcgattaatc gacatattaa
    30541 gtggctgttt ccagcctttt ttaggtgctt tggctgcagc gggaatggtc aaaggattaa
    30601 atgctttgtt cttgtttctc aaattttata ctgaaggatc gggtacgtac atcatgctga
    30661 atgggatagg ggatgcaatt ttttatttca tgccagtgat cttagggtac acagcagcga
    30721 aaaaatttca tctgaatccg atggtggggc tcgtgattgg tgcagcactt tgttatccga
    30781 ctgtacaagg agcagcacta caaagtggtt tccctgatgg cagtcctgcg ccttatagct
    30841 tgctgggtct tccggcttat gaaacatttt tgggtatccc atgggtgggc agcagttata
    30901 ctagtagcgt cgtacctgtt atcttcatta tcgcatttgc cggacaagtc caaaaaattg
    30961 ccaagaaatg gattcctgca gtcgtccaga cctttttggt ttctttttct gtgttattaa
    31021 tcgcacttcc tgctggcttt ttgattatcg gtccgattgt cagtatactg acagatctat
    31081 taagcacggg attccaagca ttgatggact tttctccagc tgtgtatggt gcagttctag
    31141 ggtttctttg gcaagtactg gtgattttcg gacttcattg gagtattatc cctcttgcga
    31201 ttatgcagct agcgcaaaat gggtggagtc agatgttagt aggcgtttat ggcgcgagtt
    31261 ttgcacagac agcagtggta ttggcgatgt atttcaagtt caaggatcaa aaaatgaagg
    31321 cgctttgcat gccagctgtg atttcaggaa tcttcggcgt aacagaaccg gctatttatg
    31381 gaatcacctt accgaagaag aagccattta tttactcgat gattggtggt gctgtttcag
    31441 gaatctattt gatggcatct ggaacaatca gctacataat gggcggatta gggatttttg
    31501 gggtggtaaa ctacatcacg gaaacagacg ccagtggtat gtttcaagcg tttatcggca
    31561 ttgctattgc ttcggtaatc gggttttcgc tgactttttt cttttggaag gatgaagcag
    31621 gtgaaacaga tgctatacag aaaaaatcag gtaaagaaat tgtaaaaaca ccgatacaag
    31681 gacaaatcgc tccactaaat gcagcgaagg atgcagcttt tgcacaagga acactaggtc
    31741 gaggaatctt aatctaccct gaaaaaggag aagtacgtgc cccttttgac ggaacagtta
    31801 tgaccctgtt cccgacgaaa catgcaatcg gaatggtttc tgaaacaggg ctggaattgc
    31861 tgattcatgt cggattggat actgtgcagt tggaagggaa atattttgaa agcttagtgc
    31921 agcaaggaga aagagtgaga caaggcgatc tgttgctacg cttcgacaaa aagaaaatcg
    31981 aaaaggaagg atacagttta gaaacacctg tgatcgtcac caattacacg gattatttgg
    32041 atattttgga aaaccaaggg gagacggtcg ggccagacga tgcacttatc accgcactga
    32101 cctgaacggt taggtgaaga atggactagg cttaactatt ctttttattc cacttcttta
    32161 ttttcaaaat tgaaaggctc tttcatgatg gctgagctga tagggcgttt atcgaatttt
    32221 tgaccgacag aaattagatg gactaaattc tgttgataat tttctgcaaa aacacaacta
    32281 tataacacgt ctagaagata acaaattgat gtattgatcg taaaattacc aatttttgaa
    32341 tacagtcgct cgcgtgtcgt aagtcgcaaa gcggctgtac tcattgcaga tagcgttgaa
    32401 tcaccgatac tggtcaaagc taaaattgga acatcatttt gtttcaatat ttctgcggta
    32461 cgaaggatca cattgttttc acccgtataa gaaatcagaa tcccgcacat atcgctcgta
    32521 caattgtacg cttcatagaa tgattcacca aaagtggtga caattgtgac atccattttg
    32581 atgcgcttca ttttcagcat gaaatcttga gaaatcaaag tattcgagtt actggcgaag
    32641 atcacgattt tttttgcctg gagcagtaaa tatttagctt tttgcagttg gtcatgatga
    32701 atgagagaaa gtgtatcatg aatcgtcatc tgttctaaag aagccatttt atttgcgatt
    32761 gtcataatac cgtctgtggt ggaaaaagga agattcgcat ctacgtcttg aaaattccct
    32821 tcaagatagg ctgtttcagc taaaaaagca ttacgaaaac tgacccagcc atcaaaaccc
    32881 attttttttg ctacacggat gagtgtagac gggtggacat aacatcgctc agctatcgct
    32941 tggatggtca tttcgccaag tgctggagaa tcgcttaaga gaaactggat cacttcattt
    33001 tctgaaggag aaaaggagat ttctttgatt ttttcacgta ataacatgta gataccttct
    33061 tcttattttt ttacgtaaat tataactttt ttgaaaatgt tttgtaaacg gatttatact
    33121 atataactta aaataggctt tttttgacaa gatatttaca aaaatccact agaaaacagt
    33181 aacttttgta aatgttactg gaaagaaacg gatttctatt gtttgttttg tttacaaaaa
    33241 agataatgaa agtgtaaaaa agtaaaggag tgagaacatg gcaaaaggaa tcaaaatcgc
    33301 tacgattggt ggaggcagca gttacacccc tgagttgatg gaaggattta tcaaaagata
    33361 tgaggaatta ccgatacgag aaatctggct cgtagatgta gaagaaggaa aagaaaagct
    33421 tgagatcgtt agtcagatgt ctcagcgtat gtgggatgct tctccatatg aagtcaagat
    33481 ctatgcaact ctggatcggg aagaagcatt gaaagatgcg gattttgtta cgactcagtt
    33541 tcgtgtcggt caattagatg cacgaatcaa agacgagcgg atccctagtt attatgggat
    33601 gattgggcaa gaaacaaatg gtgctggcgg gatattcaaa gcttttcgta ccgttccagt
    33661 cgttttggca atcgttgagg acatgaagcg tttatgtcca aatgcttggc tgatcaattt
    33721 cgcgaaccca agtgggatgg tgacagaagc agttatcaga tatggcaaat gggataaagt
    33781 gattggatta tgcaatgtgc cagtgatggc gatgatgaca gaaccagcaa tgcttggcaa
    33841 gaaaaaagaa gagctgatct acaagtttgc cggtctaaat catttccatt ggcacaaagt
    33901 agcagataat aaaggaaatg accgcacggc agaattgatt gatcgtttat acgatgaaga
    33961 taatggatta ccgaaaaata tctttgatat cccgtttttc aaagaacaat taaaacaaat
    34021 gaacatgatc ccttgtggct accaccgcta ttactatcgt gaagaagaaa tgctggaaca
    34081 tgctttagaa gaatatcaaa cgattggtac aagagcgcag caagtgaaag aaacagaagc
    34141 agagctattt gaattataca aagatccaga attagaccat aaacctgaac aattgcaaaa
    34201 acgaggtggc gcttattatt cagacgctgc ctgtgaaacg attgcttcaa tctatggaaa
    34261 taaagaaaca cagatcgttg tatctacaaa gaatgatgga gctgttcctg acctgtcggc
    34321 agattgtgtt gtagaagtca ctgcctatgt tggcgcacaa ggagcgagaa atgtagcgtt
    34381 tggttctctt cctgctgctg aaagaggctg gttgcaagta atgaagaata tggaattgct
    34441 gacgatcgaa gcagctgtga ctggtgatta tgggacagct ttacaggcgt ttacgatcaa
    34501 tccacttata cgatcaggaa agacagcaaa acggatcatg gatgagttgt ttattgcaca
    34561 ccagcagtat ttaccgcaat tcaaggaaac aatcagccga ttaaaagaag aaggaatcga
    34621 agttcaagat gaactggcaa gagaattatc aggtaataca catataatgg aggaagtata
    34681 atggggttca gaaaagattt tttatggggc ggtgctacag cagccaatca atgtgagggc
    34741 gcttatgatg aaggtggacg cggactagca aacgtagatg ttgtgccaat cggaaaagac
    34801 cgttttcctg ttatcaccgg aaagaaaaaa atgtttgaat ttgatgagga tcatttttat
    34861 ccagcaaaaa ttgcaatcga tatgtatcat cgttataaag aagatattgc tttgtttgca
    34921 gaaatggggt tcaagactta tcgaatgtct atcgcctgga gtcgtatttt tccaatgggg
    34981 gatgagaaag agccgaatga agctggcttg aaattttatg aggatgtctt taaagaatgc
    35041 caaaagcacg gaattgagcc acttgtgacg attacgcatt ttgattgtcc aatgcatttg
    35101 atcaaagaat atggtgcgtg gcgttccaga gaactcgttg gtttttatga aaatctgtgt
    35161 cgagtcatct tcaatcgcta taaaggactt gtcaaatatt ggctgacatt caatgagatc
    35221 aatatgatcc ttcatgcacc atttatgggc gcagggctgt attttgaaga aggagaaaat
    35281 gaagaacaaa tcaaatatca agcggcacat catgagttgg ttgcaagtgc catcgcaacg
    35341 aagattgctc atgaagtaga tccggagaac aaagtaggct gtatgctggc agcaggagca
    35401 tattatccat acacttgcaa tcctgaagat gtttgggctt ctagaaaatc agatcgagaa
    35461 aattatttct tcattgatgt ccagtcacga ggagaatatc ccgcttatgc attgaaagaa
    35521 atggagcgaa atggcattca gatcgaaatg gaaccagggg atgccgcatt gctgaaagaa
    35581 catactgttg attttatctc tttttcttat tattcttcac gagtttcgac taccgacaac
    35641 gaactcttgg aacagactgc aggaaatatc tttgcttctg tcaaaaatcc atatctgcaa
    35701 gcaagtgaat gggggtggca aattgatcca ttaggcttga gaataacgat gaatgatatt
    35761 tatgatcgtt atcaaaaacc attatttatt gtagaaaacg gcttaggtgc agtcgatacg
    35821 ccagatgaaa atggttatgt ggaagatgat taccgaatcg attacctggc agcgcatatc
    35881 caggcgatga aggatgcagt agaactagac ggagtcgatc tattaggcta tacgacttgg
    35941 ggatgtatcg atttagtatc agctggtacc ggtgagatga agaaaaggta cggattcatt
    36001 tatgtcgatc gtgataacca aggaaatggc acattagccc gttcaaagaa aaaatcattc
    36061 aactggtata aaaaagtaat tgattcaaat ggagaaaatc tttcgaacaa ttgatcaatg
    36121 atttataaaa gtctattgtt tttcagcgga tttttcgagt ataataaagc tattcgagaa
    36181 acgcaagcag aacaatagac tttttttcgc attggctgta aagtatgctt acttgattaa
    36241 gtaaaggaga atctacatat gtatgatgta atcgttattg gcgcaggtcc tgctgggatg
    36301 actgctgctt tgtatgcttc acgttcaaac ttatccgtat tgatgatcga acaaggtgcc
    36361 cctggcggcc aaatgaacaa tacggcagaa atcgaaaatt atcctggatt cgattcgatc
    36421 atgggccccg atctagctta taagatgtat gaaagtgtct caagatttgg gacagaaaat
    36481 gcttatggaa tcgttcaaga tatcaaggat cacggcagcc ataaagaagt catctgtgaa
    36541 gataaaagtt acgaaggaaa aacagtcatt atcgcaacag gctgtatcca ccgtaaatta
    36601 ggcgtacctg gcgaggaaga atttgctgga cgaggcgttt cttactgtgc agtctgtgat
    36661 ggtgcgtttt tccgtaacaa acgtctagta gtcatcgggg gtggggactc agcagtagaa
    36721 gaagccatct atttgacaca atttgcttct gaagtagtga tcgtccaccg tagagatgaa
    36781 ttacgtgcac aaaagatcat ccaagaccgt gcatttgcaa atgaaaaaat ttcctttatg
    36841 tgggatacag tcgttgacga aatcgttgga aacgaaatgg tagtgacagg tgtgaaagca
    36901 cgtaatgtca aaaccggcaa tgaatttgat ttagcagcag acggagtatt catctatgtt
    36961 ggactggatc cattaactgc accttttaga gaagcaggtt tgacgaacga agctggctgg
    37021 attccaacag acaacgaaat gaaaacgaat atgccaggcg tttttgccat cggagatgtt
    37081 cgtgaaaaag atttgcgaca aatcactaca gctgttggtg acggcggtat tgctggacaa
    37141 caagtgtata aatacttgga gtctttagaa gaaacaacag cataagttta aaaagagtga
    37201 aagaacggtt tcaattgttc ttttactctt ttttttcgcg tgaaatccac tgaatccgat
    37261 ttatgtgaat acttgcaatc atgcttgata aaagaatcct ttcttgattt aagcgttttt
    37321 tggtcctcat actttcatat ctaataagtg aatgctatac ttattaagaa ccatatttca
    37381 gaaagaggtg ttattttcat gtcttgggaa caagtgtatg aacaatgggt acatgaagaa
    37441 aatttagaag aaaacttaaa aaaacaatta aatgatctgg gtgaagatcc tgaaaaactt
    37501 gaagatgctt tttatgcacc gttggaattc ggtactgcag gcatgcgggg aatcttagga
    37561 cctgggatca atcgaatgaa catctatacg gtaagacaag caacagaagg actagcacgt
    37621 ttcatggata cacaaggtga agaaacaaaa cgccgaggtg tcgctatcgc ttatgactca
    37681 cgccatcagt caccagaatt tgcaatggaa gcagccaaaa cactggcaca tcacaatatc
    37741 ccgtcttatg tattcgaaag tctgcgtcca acaccagagc tttcttttgc agttcgtcat
    37801 ttgaagacgt ttgcgggaat catggtaacc gcatctcaca acccagcggc ttacaatgga
    37861 tataaagtct acggagaaga tggcggacaa atgcctcctg cagacgcaga tgcattgacg
    37921 aaatatgtcc gtgaagtcag caatccgcta aaagtcgaag tattatctga cgaagaagct
    37981 aaacatagcg ggttgatcac gatcatcgga gaagaagtgg acgctgctta tctagaagaa
    38041 atcaaagcag tgacgattga tcgtgaattg gttgaaacaa tggggaaaga tttgaaactt
    38101 gtgtacacgc cacttcatgg aacagggaaa atgttgggcg aacgtgcgtt gaaacaagct
    38161 ggattcgaac aattcgtact agttcccgaa caagctgtgg cagatccaga tttcactacg
    38221 gtcaaatcgc caaatccaga agaacattca gcttttgaat atgctatacg acttggagaa
    38281 aaagagggag ctgacttgct gattgcaaca gatcctgatg cagaccgttt aggcgcagcg
    38341 gttcgtttgc cagatggttc ttatgaagta ttgactggga accaaatcgg agcattgttt
    38401 gctcaatata ttttggaagc acatcaatca agaggcactt taccagaaaa cgcagcggta
    38461 ttgaaatcaa tcgtttccag cgaattgcct acagcaattg cggaacacta cggtgctact
    38521 atgttcaatg tgttgacagg gtttaaattc attgctgaaa aaatccaaca atttgaagaa
    38581 gaccattcac atacttttat gtttggattc gaagaaagtt atggttattt agttaaacct
    38641 tttgttcgcg ataaagacgc gatccaagca ttgcttttat tagcagaagt agcagcttac
    38701 tataaaaaac aaggcaaaac gttgtatgac ggcttgcaaa cgatttttga aacctatggc
    38761 tatttcgcag aaaaaacgat ttctgtgaca atgagcggac aagaaggttc tgcgaaaatc
    38821 tctgctttga tggaaaaatt ccgtaaagaa gcaccgacag aatttgccgg agcaaaagtt
    38881 attcaaacag aagactttaa agaattgacg agaacagacc gtgaaggaaa cgtagaaaaa
    38941 ctaacaacac cgccatccga cgtattgaag tatacattgg aagaaaatgg ctggatcgct
    39001 atccgccctt caggaactga gccgaaaatc aagttttata ttggtgtaaa aggaagctct
    39061 acagaagacg cagccgaaaa agtagcctct tatgaagcga ttatcaaaaa gatgacagaa
    39121 gaataaaaaa cttgagactg atggaaaaag tggtaaactt tagaagtcag tcttttttca
    39181 agttggaggt gaatggattg ccagacacgc aaaaatcaaa agtagagaaa gtcagtcaat
    39241 tattcaaaat gttgagtgac cccactcgtc ttaaaattct gctatatctg aaagacgggg
    39301 aacaaaatgt gacagcaatc acccaagcag tggaaatgga gcagtctgct gtatcgcatc
    39361 aactacgttt attaagagaa aatcatgtcg tcaagtctca tcgcgaggga aaagcgattt
    39421 tgtatagtct tgatgattct catgtactgg atattttgaa ccagacactc aaacatgtgg
    39481 aacatcaata acgataggtg caaatagata aacactggag tgtgacaact aaatttcttc
    39541 gttgtcacac tccagtgttt tttaagaaaa agacaaaaat gattaatcgc ttttttctgt
    39601 tgtttcagtt ccataataac tatgatcttc taggtctaaa gcattcaaaa taagcgaagc
    39661 cgtttcttca atagataaag atgccacatt gatcactaca catcctagtt tttcataaag
    39721 atcattcgca aaatcaagtt cagctctaat tttttcgata tcggaataag cagtatcagg
    39781 gtttaatccg taagagcgca tccgttcttt acggatatta tttaaaatat ctgggttatt
    39841 cgttagtccc acgatttttt taggatcgat ctcccaaagc tgtttgggaa tatgtgcttg
    39901 tgggatcaaa ggtaaattgg caacttttaa atttttgtta gctaaaaaca agctcaaagg
    39961 cgttttggaa gttcgggata cgcctagcaa aacgacatct gcttctaaaa aaccacgagg
    40021 atctttccca tcatcgtatt ttacagcaaa ttccattgct ttgatccgtt tgaaatagtt
    40081 ctcattcaat aaatggaggg caccaggttg ccgaattggc ttgataccag ttaaacgttc
    40141 aatttctgat atcggtggcg tcatcacatc aaaactaaat aattgatgtt cttcacaaaa
    40201 agaacgtgtt atctcgatca gttcttgatt gattaatgta tgcaggacaa gtgctttttc
    40261 ttctaatgca tcttgcaatg cctgccttaa tgttttttca tctgtcacga acgttctgcg
    40321 gaataggttg aactcaacgg taggatactg agccatagta gcttgcgcta gttttgtcgc
    40381 agtttctcca gcagagtcgg agatgacaaa aaaagtaacg atttctgtag aatcggaaga
    40441 catggataaa acctcacttg catttttttt atgtttatta taccatactc tagaatgcgt
    40501 acttcgtaat cattttgatt tggaggaata agtcatgaaa aaacagacat tgatcgaaca
    40561 atcattagaa acattgaaaa ataacggttt taaatatact aaaaaaagag aagcattgtt
    40621 aacttattta gtgaagcgta accgctacgt atctgctaaa gaagtttttg actatatgaa
    40681 tcaggaattc aaaggggtaa gttatgatac gatttaccgg aatctccatg attttgcaga
    40741 gatcgattta ctggaagaaa ccgatttaaa tggagaaatg aagtttcgtt ttcattgctg
    40801 tcaggggaaa atcggccatc accatcattt tatctgtaca gtgtgcggaa caacgaaaga
    40861 aatccatatg tgtccgatga attatttcga agaacagcta ggtggatgca cgatcgaagg
    40921 gcatagattc gagattttcg gaagatgtga aaattgtcaa taaggattta aataaaataa
    40981 catttgataa ttttaaaaaa aagcaagatt tccttgacgg tcaaaacagc attagatata
    41041 atatttacag gacagtcttg ttttttgaga tatatttcaa taacaattac ggttactttt
    41101 taagctcgga gggagggaat taacatgtca aaaacagtcg ttcgcaaaaa cgaatcactt
    41161 gacgatgctc ttcgtcgctt caaacgttcc gtgtcaaaag cgggtacttt acaagaatct
    41221 cgtaaacgtg aattctacga aaaaccaagt gtaagacgca agaaaaaatc tgaagcagct
    41281 agaaaacgca aaaaattcta gttctgtgat gaattggagt tggtagtgtg tcactactaa
    41341 gtagattaaa cgatgacatg aaagcagcga tgaaagcgaa agataaggaa tctttacaag
    41401 tcatccgaat gatcaagtca tctatacaaa acgagcagat taaagaaggt cacgacttga
    41461 cagaagaaga agaactaacg gtgttgtctc gtgaaatgaa acagcgacgt gattctttgc
    41521 atgagtttga agaagcagga cgtgacgatt tagctgaaaa agtaaaaagt gaaattgtaa
    41581 tagtcgaaaa atatatgccg gaacaacttt ccgacgaaga aattcgacaa cttgttcaag
    41641 aagcaatcac tcaaactggt gcttcatcaa tgaaagaatt tggcaaagtt atgggtgcaa
    41701 ttatgcctaa agtcaaagga aaagcagatg gaaaccaagt gaatgcgatt gtgaaagaac
    41761 tattacaatc ttaattttat catttaaact gcggacagtg attatccgca gttttttgtg
    41821 ttcgtaaaaa aagaactaaa aaacagctag aagaaagaat cttattcttt cttctagctg
    41881 tttttttcct gtttaatatc tcttatgtta aattgtgttt gatttggtta ttcttctggt
    41941 aactcacttg cttcttgttt atatcctgat acgcgatata cataataaaa gagcaagctg
    42001 ccagcgtaac caagcaacgt tgggacgacc cagccaagac ctaatgcagc aaaaggaaca
    42061 taatgatgat aaaatcctag taagcttgct acgacagttt gctgactcac taatgcaggc
    42121 gaatttgcca acatatccaa tacggctgga acggcagaaa agaacatcgt tacttgatag
    42181 acaaatggac tcttttggaa gaattttgct gttaaggaaa gtaagattaa cgcaagtgac
    42241 aatggataca aaagcatcaa tactggtaaa gaccattgga tgatattatc caatccggca
    42301 ttcgcaacga caaaagagac aaaagtcgtt aaccgcagcc atgtcatata actgacttta
    42361 gggaacagtt tatggaaatc ttgtgcaaaa gaaacaacga gtcccatagc agtagtgaag
    42421 acacctaatg tgaccattac ccctaagaag ctagcaccga agttacctaa ataaaaatta
    42481 gtgatctgag aaagtgcagt tccgccattt tctgataaag acatcttatt caaactcatc
    42541 gcaccgagaa ggaccagacc aaaataaatc atgacttcta acgcaacgct caggctacca
    42601 gcttttgctg ttaattctgt gactttttta tctttaaaac caagatcacg aactgcatgg
    42661 acaaaagtga cacttaaagc aagtaaagct actgcatcaa ccgtgttgta tccttctaaa
    42721 acgccatttg tcaaagcgtt cgtctgataa gaatctgcag cagattggtt tagattcccc
    42781 atgggttgga ataaagaaac gacaaaaatg atagctaata atgcaagaaa caaggagttt
    42841 aaatactttc cgacatattt gaccaaacgt gttggatgta cagtaaggaa ataagcaagt
    42901 aaaaagaaaa gagcagagaa taaaaacata cctaatgaat catatttttc aggcaaaaag
    42961 ggtttagcag ccatttcaaa agcagttgct gctgtccgcg gtgtaccaaa gaatggtccg
    43021 atcgttaaat gaacaaggat caagaacaag gcagcatacc attttccgac aggtttagct
    43081 aagtcataca gaccatcact ttttgtcact actacagcta agatagctaa taatgggaaa
    43141 agagcgcctg agatggcaaa tcctacacca gcgctgatcc agtttgatcc agccatttga
    43201 cctaagtgta caggaaagat cagattacct gacccgaaga acatcccgaa gataagtgaa
    43261 ctgaccatca ataattgctt ccatgaataa gtttttgttt cttccataaa tttttcctcc
    43321 aataattatc attttgagta acaaaataaa cgttgcagaa actagaaata ccactaatat
    43381 cagattccat gaaaaccacc accttaaaat ataaaaaaag cccttttcca cttgggaaaa
    43441 gggctctaga ttctagaacg gtaccacctt ttttcgctta aaaaagcttc ttcattcacg
    43501 gacgcttttc gataaagtta tccgctagcc tgataatgga ggcagccaca atgatctacg
    43561 ttttcgacat tgactcggaa atgatgttcg tctattcgat ttattgtcat ttctcactta
    43621 catgactcac tgggaataaa tcactgaata gcctactgtt ttcctcgacg ctttgctgta
    43681 tatttatttg ttgacgttat aatacacctg ttttttttga aatgcaagaa tatttcaaat
    43741 attttgtttt ctttttttga aacaagaaaa atacccagag aatacttgct aagttagacg
    43801 gaatacttgt cctctatctt atgcatagct tacatgatca gcttttttta ttcaaacgag
    43861 acctttagag aatggaagcg gctagttttt taacgtttct tggtacttat ggttaagcaa
    43921 ctcttttaga accttttctt atttgctata tagtttcttc tgagtatggt aaaataggaa
    43981 agaatgaatc gaaaaaaagg ggatgtctat ttttgacgga agagtctagt tctttagaga
    44041 ttagattgac ggctgctgat gatgtcagca tgcttttagg tgcacacgac aagcatataa
    44101 aactgattga ggaaacaacg gaaacaacca tccatacacg tggagaaatc attcagatca
    44161 ttggtgagca atcccagatt tccttggctg cctcagtcat tcggacatta caggaattga
    44221 tcaaacgagg aatccacgta agtacgcccg atgttgtgac ggcattgaaa atggggcaaa
    44281 aaggtacatt agattatttt gttgagatgt atgaggaaga gatcgtcaaa gaccgtaatg
    44341 gaaaacctat acgggtaaaa aacagcggcc aaaagaaata tgtcgaatcg gtagccaaac
    44401 atgatatcgt atttggcgta ggaccagccg gtacaggaaa aacttttctt gcagtagtat
    44461 tagcaatcgc tgccttgaaa aaaggtgaag tgcaaaaaat cattttgacg cggcctgctg
    44521 tggaagcagg tgaaaatcta ggctttcttc ccggggatct aaaagaaaaa gtggatcctt
    44581 atctgcggcc tgtttatgat gctttatacc aaatttttgg attggatcat acgaaccgat
    44641 tgatggaacg aggcgtaatc gaaatcgcac ctttagctta catgcggggg cgaacattgg
    44701 atgatgcatt cgttatcttg gacgaagcac aaaacacgac agtcgctcag atgaaaatgt
    44761 tcttgacccg tctcggttat caatcaaaaa tgattgtcaa tggggataca agtcagatag
    44821 acttgcctcg agggacgaca agtggtcttg ttcatgcaga acgaacatta aagcaaatca
    44881 agaaaatcga ttttgttaat tttgaagcaa gtgacgttgt tcggcaccct gtagttgccg
    44941 aaatcatcaa agcttatgaa aaggcagatc tccatcaaga ataagctttg cttaggagga
    45001 taagcacatg ttgaataaaa cactgcagaa actacaagaa aaacttggaa aggcagcatt
    45061 gcctttcctg ctgttcttgt tttttatcat tatctgcggc gttacattta gtagtgtcaa
    45121 acaaaaggcc aatgatttca gagaaggcca agtagcagaa gaaagtatcc gtgcgaacaa
    45181 aacgatcgag aacacggaag aaaccgaaca aaaaagaaaa ctggcagcag aagcagtcac
    45241 tccagaatac acttaccaaa aagatcttgc agatgaccag aacaatcgga tcaagcaact
    45301 atttgaattg atcgataaga cgaatgatgc catcaacaaa tcttatgacg aaaagattga
    45361 taaggcaaag gaagatgaaa atatcccaaa gcctactaca gaagagcgga tcgccgcgct
    45421 gaagaaaaat tttgaaaatg tgaatgcaga aaatgttgtg ttttatcaaa aactaccgaa
    45481 caatttttat tcgacgatat ttgaaatgac tgaaactgag attcatactg tgagggatga
    45541 gagtctgaag ctcattgacg aacagatgag caaacaagtt cgtgaaagtg aattggaagc
    45601 ttttaaacaa gaagcagaag agcaaatcca atatttgaat gtcacaccag cacaacaaca
    45661 aatgattcgc tacttagtgg atcaaggtat cgtagtgaat gacgtattga atgagaaaaa
    45721 gacagaagaa ttgaaacaat ccgcacgaga agctgtacaa ccagtgatga tcttccaagg
    45781 agaaatcatt gttcgtgaag gtaatcaaat cgatgcttca gctatgaaaa aattggaatt
    45841 actaggactg actaatcaaa caacttcgat ttttccgcta attgccatgg ttttagctgt
    45901 tttgcttcaa atcggcgtac tgatttataa ttcacttcaa ttccaaaatg caggagaaag
    45961 aacaaaatac gtgcttttct atgtgacagc tatgtcagtc agcatagtgc tgatgaaatt
    46021 tttccaatta ttccaaacgg aacaagctac ttatatcccg ctgttttatc ccgctgcttt
    46081 tgcaccactt gttttaagct ttttcttgaa tcgaagagca gggatcatgg ctggtatttt
    46141 ccaagcagta tccgcacttt tcatttttta tgattcgatt ggcacgaact ttctaacgat
    46201 tatcttgatg tcttatgttt tctctgggct catggcaacg attgtcagac gcaaacggat
    46261 ctcagaacaa ggattatctg ctgccatgtg ggtcattatt tttcctgtga tgatggatgt
    46321 cattttgatc gtctatcaag gaatgagctt tggtgatgca acgacttggt taatgctcgt
    46381 ctgcgggttc actggtgcac tcttctcatt cttgctgacg atcggtctac acccatatat
    46441 tgaattgatg gtcaatgatg acagtgtaat tgtattaaat gaactcagca atccaaatca
    46501 tcctttattg aaacagttgc tggaagaagc gccaggtact tatcatcata gtatgatggt
    46561 agctaatcta agtgctaacg ctgtagcaga aatcggcgga cgttctttgc taactcgagt
    46621 agcttgctat tatcatgata tcggcaagat caaacatgct agctttttcg ttgaaaactt
    46681 gccttcagga gcggaaaacc cgcacaattt cttattgcca gaagacagta aacatattat
    46741 ctttggacat gtgacagatg gagccaaaat attggaagat tataatatgc cgcaaatggt
    46801 gatcgatatt tgcaaacagc atcatggaac cacgctaatg cgttatttct acatcaaagc
    46861 gaaagaacgc aatccggaag taacagaaga gcaattccgt tatcctggac caaaaccgca
    46921 aagtcgtgaa gcagggatcg tcaatattgc tgacagttgt gaggctgcag tacgggcaat
    46981 ggatcatccg acgagcgaaa aaatcgaaag ttttgtccat aatcttatcg aagagcgtat
    47041 ttccgatggt caattagacg atagtggact gaccttgaaa gaaatccgaa aagtggaaaa
    47101 atcattaatc agcggtttaa gtagcacctt ccattctcgg atcaaatatc ctaaaatgaa
    47161 aacagaagtt gagaagataa aggaagaaca agaagaatag aaagaagcga tgtataatgg
    47221 atattacctt tatggatgaa acaaaccaag tctcagaaga gaaaatgaaa gaaatcgatg
    47281 acttgctgca atttgcagca tcttatttaa agctgccaga ggaaacagaa atgtctgtta
    47341 ctttcatgga caatgcggca atccaagtaa tcaatcgtga ttaccgtgga aaagatgcac
    47401 cgacagacgt catcagtttt gctctggaag aagaaggcga agacgaaatc ccagtgattt
    47461 ttgaagatgg agagaatcca ttacctcgtg tactaggaga catcatgatc tctattgaac
    47521 gtgctgctga acaagcagaa gcttacggcc attcatatga tcgggaactt ggctttttag
    47581 cggtacatgg attcttgcat atcaacgggt atgaccatat gacaccagaa gatgagaaag
    47641 aaatgtttgg tttacagaaa gagatcttgg atgcctatgg actcaaaaga taaaaacgta
    47701 gagaaaaaca aacatttcct gatgtctgtg gaatttgctg tccaaggagt caaaaccgtt
    47761 tttgaggaag aacgcaatat gaggaaacat gttgcgttcg gtatccttgc tttatttatg
    47821 ggcgtgattt tccagcttga tcggatagag tggttatggc tgcttttagc tgtttttctc
    47881 gtttggatag tagagatcat caatactgtt tttgaaaatg tagtggacat gtttacggat
    47941 tttcattttc atccgattgg gaaaaaaatc aaagatatgg cagctggagc agttttagtg
    48001 accgcttgtt ttgctgtcat cattgggttg attttatttg tccctaaaat atatcatttg
    48061 ttgtttaatt aaaaaggaga acgtatatgc cagaacataa atcagggttt gtcgcaatcg
    48121 tcggccgccc gaatgtagga aaatccacat tattgaaccg aatcgtgggt caaaaaattg
    48181 ccattatgag tgacaaggca cagacaacta gaaataaaat ccaaggagta tatactacac
    48241 cagaagctca gctgattttt atcgatacac caggaatcca taaaccaaag catcgtctag
    48301 gagattttat ggtagaagca gcttacagtg cgttgaaaga agtggatgca gtcttattta
    48361 tgatcagtgc tgatcaaaaa cgtggccggg gagacgattt tattattgaa cgtctaaaaa
    48421 acgtgcattc tcctgtttat ctgattatca ataaaatcga taagatccat ccagacgagt
    48481 tgctgggaat catcgaagat tattcgacgc agatgccttt tgcacaagtg attccgatct
    48541 ctgctacaga aggaaacaat gtcgaacgct taatggatgt attagtcagt gaaatgccag
    48601 agggcccaca atattttcca gatgatcaag tgactgatca tcctgaatac ttcatcgttt
    48661 ctgaattgat ccgtgagaaa gtcttgtttt taacacgaga cgaagtgcct cattctgtcg
    48721 cagtcgttgt tgattcgatg aagcgaaacg agaatgataa aatccatatc caggccacga
    48781 tcatcgtcga aagagatagc caaaaaggaa tcattatcgg caaaggcgga aaaatgctga
    48841 agcaagtagg aacgaaagca agaaaggata tcgaaaatct tttaggagac aaagtatttc
    48901 tagaactttg ggtcaaagtc caaaaagact ggcgagacaa aaaagtctat ctgcaagact
    48961 tcggctaccg aaaagacgag tactgatatt ctggacttct tataggttag gtatagaaat
    49021 aaactggata aaaatccgta cgttgagaaa aacagcggaa gatcgaaaca taaataatct
    49081 ggatcggcag aatctgcttt tccagattta ttttttagga gggagaatat gagccagctt
    49141 gcagaatcga aaggcttgat tttatctagc cgtaattaca aagaaaaaga taaattagtc
    49201 aaaatcttta cggaatcttc tggcaaaatg atgttttata tcaaaggaat ccatcggcaa
    49261 aatcagccat tagctccggc tttgctcccc tttaccgaag cagtctatat cggtaatttt
    49321 cgaagtgaag gtttgtcgtt tttaagcagt gtcaaagacg tccatgcgtt tcggaacatc
    49381 caagaagata tctttttgag cggttatggt acgtatttgc taaatctagt agatgcagca
    49441 atcgaagatc atcaatatga cccgcattta ttccaattta cgcaacaagc tctgcagcgg
    49501 atggaccaag gagatgatgc agaaatcatc acgaatatct ttgaagtgca aatactgcaa
    49561 cgcttcggaa tcgcgccaat ttggacgcac tgtgtcgtat gcggggaaac aaaaggaaaa
    49621 tttgactatt cttccaaata tggcggtgtg atatgcgaga aacactggcc aatggacgaa
    49681 caccgttatc atgcagatcc tcgcgctgtt tatttcgttc gaatgttttc ggctatttct
    49741 tatgataaga tcagtggaat ccacttgaaa gaagaaacga agcaggcaat ccgtgacctc
    49801 attgaccagt tgtatgatga atatgtcggt attcatctaa aaagcaaaaa gttcattgat
    49861 caaatgaagt cttgggcaca cgtattaaag acgccagaga aaaatatgcc tgaaaaagag
    49921 atacctgaaa aagaagaata aatgaaagat acaacaaaaa aatcaaaact atttgacaaa
    49981 tacagggact tccgtctatt attaaccgta gattaaatag atcgttcact gagaggatct
    50041 attatacaaa ccagaaaact gcaaaatgaa tacgtacatt gaccaagatc agtaaaaaac
    50101 acgtggttta gcgagttcgg gagagtggaa gccggaccca aacgattttt gaagaggcac
    50161 ttgcgagtac gataaatgaa gctgccgaat ctttattcgg aagtagggtg gaaccgcgaa
    50221 attattcgtc cctatgtctg taatcagaca tagggacttt ttgtcgtttt ctttcctgta
    50281 ttcttattga cagcaacgct taaaaattga aaggggcaaa acagatgagt aaaaaattga
    50341 ccgtccaaga tatgatttta acattacaaa aattttggtc atcaaacggc tgcatgctga
    50401 tgcaagctta tgatacagaa aaaggagcag gtacgatgag cccatataca ttcttacggg
    50461 caatcggacc ggaaccatgg aatgctgctt atgtagagcc atcccgccga ccagctgacg
    50521 ggcgttatgg agaaaaccca aaccgtttgt atcagcatca tcaatttcaa gtcgtaatga
    50581 aaccttcacc tgaaaatatc caagagcttt atttagaaag cttgcgtctt ttaggaattg
    50641 atccattaga acacgatatt cgctttgttg aagacaactg ggagaaccca tcgatgggtt
    50701 gtgccggtgt gggctgggaa gtatggcttg atgggatgga gatcacacaa ttcacatact
    50761 tccagcaagt cggaggacta ccttgtaaac cagttacttc tgagatcact tatggactag
    50821 aacgtcttgc ttcttatatc caagaagttg aaagtgttta tgatctggaa tgggctgatg
    50881 gcgtgaaata tggtgagatc ttcaaacaac cagaatatga acattctaaa tattcttttg
    50941 aagtaagcga ccaagacctg ctactaagca attttgatcg ttttgaaaaa gaagctaaac
    51001 gctgtatcga tgaaaatctt gttcatccag cgtatgatta tattttgaaa tgtagccata
    51061 cattcaattt attagatgct cgcggtgcag tatccgtaac agagcgtgca ggttatcttt
    51121 cccgtatccg caacatggct cgtgctgtag ccaaaatctt tgtggcagaa cgcgaaaaat
    51181 taggattccc tttattgaat aaaaacacag caaatccaga aaacgtgaag gaggctgaat
    51241 aagatggcaa aagacttatt acttgaaatc gggctggaag aaatacctgc tcatgtagtc
    51301 acaccaagca tgaaacaatt agaagaaaaa acagcaaaat ttttagatga aaaccaattg
    51361 acttatgata cgatcgaaac gttttctaca ccgcgccgct tagcaatcaa agtaacaaac
    51421 attccagaac gtcaagcaga cagcgaagaa gaagtcaaag gaccagccaa aaaaattgct
    51481 ttggacgcag atggaaactg gacgaaagca gctgaaggat ttgtccgcgg acaaggtctg
    51541 acgacagatg ctatcacttt tagagaatta aaaggcgtgg aatatgtcta tgtaacgaaa
    51601 tacactcacg gcaaagaagc aaaagaagta ttgacaggtt tagcagatgt cattaaaagc
    51661 ctcactttcc cagtaacgat gcactgggca gaatacgatt ttgaatatat tcgccctatt
    51721 cactggatcg tggcattgtt agatgacgaa ataatcccgt ttgagatctt ggatgtcaaa
    51781 acagacagag cttcaagagg tcatcgtttc ttaggcgaag atgtgacatt tacgcatgca
    51841 aaacaatacg aagaaaaatt gaatgaacaa tttgttattg cttcaccgca aaaaagaaaa
    51901 gaaatgatca tcacacaagc aaacgaatta gcagaaaaaa atcactggat cattgatcta
    51961 gatgaagatt tactagaaga agtcaacaat ttagtcgaat atccaactgt cttttctggc
    52021 gacttcgaag aaaaatatct atctgttcct gaagaagtac tagtcacttc tatgaaagaa
    52081 catcaacgtt atttcgaagt acggaacgat caaggtatgt tgcttcctca ttttatttct
    52141 gctcgtaatg gcgatcgggt aaaattggaa aatgtggtac gaggcaatca aaaagtattg
    52201 actgctcgtt tagaagatgc ggaattcttt tataatgaag acaagaaagc ttcaatcgac
    52261 caatacgttg aaaaattaaa aactgttact ttccatgaaa aaatcggtag catctatgag
    52321 aaaatgcaac gggtacaagt catcgctcgc atcataggcg aacaagtagg actatctgag
    52381 gaagaattga tggatctgca acgcgctgct gaaatctata aatttgatct agtaaccaac
    52441 atggtaggag aattccctga actacaaggg atcatgggcg aaaaatatgc tctgcttaaa
    52501 ggcgagaaac cagccgtagc aacagcgatc cgtgaacatt acatgcctat atccagtgaa
    52561 ggagatttac ctgagactac agtaggttct gttttagcga ttgctgataa attagatagt
    52621 atcttttctt tctttgcagt tgggatgatc ccaagtggct caaacgatcc atatgctttg
    52681 cgccgtcaag catatggtgt cgttcgaatc atcgaatcga aaaaatgggc ctttcctttg
    52741 tctgttctgc aagaaacgat tagtgaagtt atcagtaaag atacggatcg ttttggcatt
    52801 ggcttgtcag cgggacaaca acaggtgatc gactttatca aaggccgtct gcgccaatta
    52861 ttgacaacga agaatatccg tcatgatgtc atcgaagctg ttttgaatgc agaacaaaaa
    52921 gatctgacaa aagtatttgc agcagcacaa ttgttcaagc agcatcttgc agatgaagac
    52981 ttcaaaccat cgatggaagc tttgacacgt gtcgtgaact tagcgaaaaa agcggagctt
    53041 gaaaaacaat cagaagtaga ccctgaactg ttcgaaaacg aagcggagaa agaactgcac
    53101 aaagcagtag aggcaatgaa aacaagcttt gctgaccaaa cgatcaacga aaattatgat
    53161 cagcttgtaa gccttcgccc actgatcgaa aattattttg atcagacgat ggtcatggca
    53221 gaagaaccag cagtacgtaa gaaccgatta acgcaattaa gtcaaattgc tgccatggct
    53281 ttgtcactag caagtttaga tcaaatcatc acaaaataat ttctaatatg taaataaacc
    53341 agagaacagg cacaaaatag gctatttctc tggtttattt gttataaata taaataacta
    53401 ttattaattc aatgttaatt tttttgttat tttatttttt gtgtaacttt taaaaacacc
    53461 t
//
LOCUS       DAZAID010000008        52977 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000008 DAZAID010000000
VERSION     DAZAID010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.008"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(202..780)
                     /locus_tag="ACG9BE_000405"
     CDS             complement(202..780)
                     /locus_tag="ACG9BE_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3031188.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(800..1468)
                     /locus_tag="ACG9BE_000406"
     CDS             complement(800..1468)
                     /locus_tag="ACG9BE_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3031189.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1483..2553)
                     /locus_tag="ACG9BE_000407"
     CDS             complement(1483..2553)
                     /locus_tag="ACG9BE_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3031190.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2724..4088)
                     /locus_tag="ACG9BE_000408"
     CDS             complement(2724..4088)
                     /locus_tag="ACG9BE_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFN3031191.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(4085..5644)
                     /locus_tag="ACG9BE_000409"
     CDS             complement(4085..5644)
                     /locus_tag="ACG9BE_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3031192.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(5700..8180)
                     /locus_tag="ACG9BE_000410"
     CDS             complement(5700..8180)
                     /locus_tag="ACG9BE_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031193.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(8164..8499)
                     /locus_tag="ACG9BE_000411"
     CDS             complement(8164..8499)
                     /locus_tag="ACG9BE_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031194.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(8513..9547)
                     /locus_tag="ACG9BE_000412"
     CDS             complement(8513..9547)
                     /locus_tag="ACG9BE_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFN3031195.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(9637..10317)
                     /locus_tag="ACG9BE_000413"
     CDS             complement(9637..10317)
                     /locus_tag="ACG9BE_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN3031196.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(10601..11704)
                     /locus_tag="ACG9BE_000414"
     CDS             complement(10601..11704)
                     /locus_tag="ACG9BE_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFN3031197.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(11926..12396)
                     /locus_tag="ACG9BE_000415"
     CDS             complement(11926..12396)
                     /locus_tag="ACG9BE_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFN3031198.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            12729..14369
                     /locus_tag="ACG9BE_000416"
     CDS             12729..14369
                     /locus_tag="ACG9BE_000416"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFN3031199.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            14523..15845
                     /locus_tag="ACG9BE_000417"
     CDS             14523..15845
                     /locus_tag="ACG9BE_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFN3031200.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(15907..16338)
                     /locus_tag="ACG9BE_000418"
     CDS             complement(15907..16338)
                     /locus_tag="ACG9BE_000418"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3031201.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(16402..17238)
                     /locus_tag="ACG9BE_000419"
     CDS             complement(16402..17238)
                     /locus_tag="ACG9BE_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFN3031202.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(17980..18606)
                     /locus_tag="ACG9BE_000420"
     CDS             complement(17980..18606)
                     /locus_tag="ACG9BE_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031203.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(18901..19236)
                     /locus_tag="ACG9BE_000421"
     CDS             complement(18901..19236)
                     /locus_tag="ACG9BE_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN3031204.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(19223..19507)
                     /locus_tag="ACG9BE_000422"
     CDS             complement(19223..19507)
                     /locus_tag="ACG9BE_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN3031205.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(19563..21086)
                     /locus_tag="ACG9BE_000423"
     CDS             complement(19563..21086)
                     /locus_tag="ACG9BE_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN3031206.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(21079..22254)
                     /locus_tag="ACG9BE_000424"
     CDS             complement(21079..22254)
                     /locus_tag="ACG9BE_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN3031207.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(22258..22443)
                     /locus_tag="ACG9BE_000425"
     CDS             complement(22258..22443)
                     /locus_tag="ACG9BE_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031208.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(22409..24103)
                     /locus_tag="ACG9BE_000426"
     CDS             complement(22409..24103)
                     /locus_tag="ACG9BE_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN3031209.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(24100..24573)
                     /locus_tag="ACG9BE_000427"
     CDS             complement(24100..24573)
                     /locus_tag="ACG9BE_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFN3031210.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(24642..24797)
                     /locus_tag="ACG9BE_000428"
     CDS             complement(24642..24797)
                     /locus_tag="ACG9BE_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFN3031211.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(24931..25311)
                     /locus_tag="ACG9BE_000429"
     CDS             complement(24931..25311)
                     /locus_tag="ACG9BE_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN3031212.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(25315..25530)
                     /locus_tag="ACG9BE_000430"
     CDS             complement(25315..25530)
                     /locus_tag="ACG9BE_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031213.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(25533..25940)
                     /locus_tag="ACG9BE_000431"
     CDS             complement(25533..25940)
                     /locus_tag="ACG9BE_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFN3031214.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(26206..27681)
                     /locus_tag="ACG9BE_000432"
     CDS             complement(26206..27681)
                     /locus_tag="ACG9BE_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFN3031215.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(27671..28519)
                     /locus_tag="ACG9BE_000433"
     CDS             complement(27671..28519)
                     /locus_tag="ACG9BE_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFN3031216.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(28556..28918)
                     /locus_tag="ACG9BE_000434"
     CDS             complement(28556..28918)
                     /locus_tag="ACG9BE_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031217.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(28919..29077)
                     /locus_tag="ACG9BE_000435"
     CDS             complement(28919..29077)
                     /locus_tag="ACG9BE_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031218.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(29218..29842)
                     /locus_tag="ACG9BE_000436"
                     /pseudo
     CDS             complement(29218..29842)
                     /locus_tag="ACG9BE_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(29839..30150)
                     /locus_tag="ACG9BE_000437"
     CDS             complement(29839..30150)
                     /locus_tag="ACG9BE_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031219.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(30194..30487)
                     /locus_tag="ACG9BE_000438"
     CDS             complement(30194..30487)
                     /locus_tag="ACG9BE_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031220.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            30681..31328
                     /locus_tag="ACG9BE_000439"
     CDS             30681..31328
                     /locus_tag="ACG9BE_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3031221.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            31389..32534
                     /locus_tag="ACG9BE_000440"
     CDS             31389..32534
                     /locus_tag="ACG9BE_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3031222.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(32615..32920)
                     /locus_tag="ACG9BE_000441"
     CDS             complement(32615..32920)
                     /locus_tag="ACG9BE_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFN3031223.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(32945..33376)
                     /gene="ruvX"
                     /locus_tag="ACG9BE_000442"
     CDS             complement(32945..33376)
                     /gene="ruvX"
                     /locus_tag="ACG9BE_000442"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFN3031224.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(33373..33639)
                     /locus_tag="ACG9BE_000443"
     CDS             complement(33373..33639)
                     /locus_tag="ACG9BE_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFN3031225.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(33894..34619)
                     /locus_tag="ACG9BE_000444"
     CDS             complement(33894..34619)
                     /locus_tag="ACG9BE_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFN3031226.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(34980..35050)
                     /locus_tag="ACG9BE_000445"
     tRNA            complement(34980..35050)
                     /locus_tag="ACG9BE_000445"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35016..35018),aa:Gly,seq:ccc)
     gene            35167..35919
                     /locus_tag="ACG9BE_000446"
     CDS             35167..35919
                     /locus_tag="ACG9BE_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFN3031227.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            35935..36414
                     /locus_tag="ACG9BE_000447"
     CDS             35935..36414
                     /locus_tag="ACG9BE_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFN3031228.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(36665..38245)
                     /locus_tag="ACG9BE_000448"
     CDS             complement(36665..38245)
                     /locus_tag="ACG9BE_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFN3031229.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(38238..38981)
                     /locus_tag="ACG9BE_000449"
     CDS             complement(38238..38981)
                     /locus_tag="ACG9BE_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3031230.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(39157..39933)
                     /locus_tag="ACG9BE_000450"
     CDS             complement(39157..39933)
                     /locus_tag="ACG9BE_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN3031231.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(40181..41017)
                     /locus_tag="ACG9BE_000451"
     CDS             complement(40181..41017)
                     /locus_tag="ACG9BE_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFN3031232.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(41028..41957)
                     /locus_tag="ACG9BE_000452"
     CDS             complement(41028..41957)
                     /locus_tag="ACG9BE_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFN3031233.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            42149..42502
                     /locus_tag="ACG9BE_000453"
     CDS             42149..42502
                     /locus_tag="ACG9BE_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN3031234.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(42576..43232)
                     /locus_tag="ACG9BE_000454"
     CDS             complement(42576..43232)
                     /locus_tag="ACG9BE_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFN3031235.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            43458..45392
                     /locus_tag="ACG9BE_000455"
     CDS             43458..45392
                     /locus_tag="ACG9BE_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN3031236.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(45478..46701)
                     /locus_tag="ACG9BE_000456"
     CDS             complement(45478..46701)
                     /locus_tag="ACG9BE_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN3031237.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(46695..48566)
                     /locus_tag="ACG9BE_000457"
     CDS             complement(46695..48566)
                     /locus_tag="ACG9BE_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFN3031238.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(48587..49705)
                     /locus_tag="ACG9BE_000458"
     CDS             complement(48587..49705)
                     /locus_tag="ACG9BE_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN3031239.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(49788..50621)
                     /locus_tag="ACG9BE_000459"
     CDS             complement(49788..50621)
                     /locus_tag="ACG9BE_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3031240.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(50614..51531)
                     /locus_tag="ACG9BE_000460"
     CDS             complement(50614..51531)
                     /locus_tag="ACG9BE_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3031241.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(51758..52882)
                     /locus_tag="ACG9BE_000461"
     CDS             complement(51758..52882)
                     /locus_tag="ACG9BE_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN3031242.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        15768 a        10730 c         8889 g        17590 t
ORIGIN      
        1 tctacaaaca attaatttct tcgttattaa attaaaacac ttcctggaac accgacataa
       61 taaataaaaa tataccctga taatcatgag agaataacaa atttgtattt atgaaaaact
      121 acctatataa agaagcaaga tataacgatc ttcgtcatat cttgcttctt cacctaacta
      181 ttttctccct atataacagc tttacgcttc tcctaataca ccattcaaat ataaatcgat
      241 caattgcgcc acatttagcg gttcttccac aggtcttttc gaccgagtga tgtaaggtgc
      301 taacactaag aaaaaatgcc ttgctgcgat ttcttttgaa atcccatttc ttagcacaga
      361 ttggtttgct gaaaagattt cttcaaaagg ttcacgataa tttttcttcc atagaaagaa
      421 aagatgatct cttgtttcct ttgaaagatt gctttcaaga tcgtgaagca tcagaaacaa
      481 atcgaccaga tgtgtctcaa tcaaaaactg cgccattttt gtcaaggtta cttgaaatcc
      541 cgtttcgctt gtttgaacga tttctctcaa agcttttcca acatctttta agtggttttc
      601 taaaactgcc gtatatagtt tttctttatc accgaaataa tgatagagat tgggctgggt
      661 gatcccaact ttttgtgcga tttctcttgt tgaagtgccg agatatccct tggacataaa
      721 caagtcagtc gcttctttca aaattttttg ctgtgtatct ttgttgtatc gatggaccat
      781 ttttccacct gctttttggt tattcttttt ctttcaatac accatcttcc atcataaata
      841 ttctatcgca tttgtcaatc agccgtgtat cgtgagtaac catgatcgtt gctttatttt
      901 tgtcttttga ttcttttgcc aagatatcga ctacttctcg tgccttttct gaatccaaac
      961 tagcagttgg ttcatctgct agaataatgg acggatcatt gtacaatgca cgagcgattg
     1021 cgaccctttg ccgttctcca ccagatagtt cttctggata cttgttgatc aacttatcca
     1081 cgccaagctg ctcaaataat tcgtttgctt ctttttgacg atgttcatgt ttaatcttat
     1141 ctactaataa taactgcttt tttactgtca gaaaaggaac cagattggaa gcttgcaaaa
     1201 taaagccaat ttctttaaaa cgtagtttaa cgcgttcgct ttctttttga ttactgaatt
     1261 cttgatcatt gataaataca gagccactgc taggcgtctg caatccgcct gccaaagtca
     1321 aaaaagtact tttacctgaa ccagaaggac cgatgattgc aacaaattcc cctttatcga
     1381 tactcatggt tgtttctttc aatgctttaa ctacttggtc gccatcttgg aaactttttt
     1441 ctacttcttt aaattctatt gctttcatgt tcttcctcct tcttatccaa ttgccttcaa
     1501 cggatcgatt tttacgattg tccgaacaga aaatagtgcg cctaacacag ctactactaa
     1561 catcaacaag ctaatgacgc caaaaaacag ccaattgctt tggaatggaa ccgcatcagg
     1621 tagaacaagg gatgttccca ctgttcctaa tagaccaaga agaataccaa caaatgataa
     1681 aatgaatgtt tgtgccacta cagaacgcgc aataaatcct cctgtgatcc cttgtgcttt
     1741 cattacacca aagatattaa tcttttgcat cgtcagtaca taaatgaaaa tcccgatcac
     1801 aatagccgca ataacgatca aaaatccgat cataaatcca aaagtcaata cttgcgcatt
     1861 atatccaggt aattcattga tgaatttaga aattttgatc tgttctaaat cttcaggtaa
     1921 attactgatt ttacctctga caactattgc gttaatccgt gcattttcac ttgtatcttc
     1981 tttttcgaag cggatttctt ggtaagcatt gatggttgta tagagaactg gtgagacatt
     2041 gaatttggca tgatccgtaa agccagttag tttaaacgtc ttgtcactac cagataactt
     2101 aactgtatct cctatggcta gaccatattc ttctttcaaa ctaatatcgc ctactgcctc
     2161 atcatcgtta tcaaaagctt ttccttcaac aagattgggc atgatgaatt gatttttatc
     2221 gataccgaag aagctaacat tgatttttcc ttcttctgta ctgtctttac tagtcacgac
     2281 tccaggtgtt tgggcaagat acgccacttt cccaccttcg acttcttctg ctatcttctt
     2341 agtaatcatg gacatcccta gatttgaatt tgcatctttc gaaagcacaa tcgaatctgc
     2401 ctcccacttg tcgactgctg ttcgattatc ttgagctaag ccataagcta acccagtcaa
     2461 aaaaaatacc aggtaggcga tcaaaaacat cacccctgca actaacgcat aacgtaactt
     2521 tgaatgcata atttcattta atgctaaaaa catttaactc ctcctcctga aataatcatt
     2581 ttatacaaca attttatcac ttgataaatt tttggcgaat attatgctca aaaaattctt
     2641 ccgctatttc attatatgaa ttttttattc tcgattgatt aatatttatt atatccccac
     2701 acaataaaag accgtgatat ttctcacagt cttttatcta ctctattgct atttgctctc
     2761 ttagctagta ctttaaaagc taactcattg aggtagtgaa aacgagaggt gtattcagac
     2821 agaaatccaa aatcctcaac tgctagctgt ggaatcgtag ttttgtgttt cacgatatcc
     2881 gcaagcaggt tcaaatctga taataagaat tcttggctta tcagctgtcc gccaattatt
     2941 tctaatgtat ccggatggta gatcagcttt gcatttactg ttttgttttc ttcgtcgtat
     3001 tgtggaaata gatcaaattc tcctgaacac gaaccggtat tttgttccaa gaatggcgct
     3061 tcatcttctg tcagccctac agttcctaaa aagtagtcta taatgactgc tgctgttatc
     3121 ttttgtctga ggtcgtaagc ggttggctga ccactcaaag ttagcgctac tgctcttgcc
     3181 atatgtacag ctcgagtaac taaaggtaaa aagagatgcc gtttattttt gtcaaaactg
     3241 actgggacga tatctccaat cgcatagatg tccggatcac ttgtctgcag atattcattg
     3301 actatcacag tctcatcctc atacaaagcc actttttctt ttaacaatgt cgtattagga
     3361 cgagtttggg cagaaaaaat gaccatatct gcctttattg tttgagacag aagttgtacg
     3421 gatactactt tctcttctga ttttttccaa tcgatcaaga attcatttgg ataaaaattt
     3481 atttgcttag tttgcagctg attttgaaaa ctgttgatca tatccttatc gaaatatctt
     3541 gataaaacat tcggcatata atcaactaga tgaataatgt atcctttatt tctcaaagca
     3601 tctgctaatt ccaatccaat gtaaccagca ccgaagatca ctacttcttt tgcttctttt
     3661 aaagcggcaa gtacttgctt agattgcact agatttttat aggtgtagac attttcagct
     3721 tcaatttgtt tatacaaggt gacaggagag gaacctgtcg ctacgattaa tttatcatag
     3781 gttatggtct cgttttttcc ttctttggat gcgaggatga gctgatgttt gtctggatca
     3841 agttcagtta ctctccattc ggtgatcagt tttgccccac tttcttgaag agtccgcatg
     3901 ttatgggcaa cttcagctaa actacgtatc tttcctttta aataaaggtt gattccattt
     3961 gaaacatagc ctagtccatc tttatgatct tcatcgatca gtaaaacttc tgctttcggt
     4021 tgaagttttc ttagatttag ggcagctgtg atgccttcat gggaagcacc aatgatcacg
     4081 attttcatgt tttattcatc cttaaattca atttttctaa ataacattct tttaaaaatt
     4141 ggttcaaata ggggaaatca taattatatt cgttgctagt gaatacaaaa actttagcgg
     4201 acttctgtga cgggtactct ttcaattggc tagtcaaaat gatatcatac tgcttttctt
     4261 tctcgtattc gactaaatca tatttgatcg tattcagcaa ttgttcctgg atgatttctt
     4321 tcacgatatc cgccattagc ctttcataag gaagatcata agcaatttgc agacgtaatt
     4381 ctaactgctt gatcagcgtc cgtaaaatag atgcatagcg agcaaataat tcaaagcggg
     4441 cattttcctc gtctacttca gtaaaatcaa caatagcaaa tgtttgtctg accaatcttt
     4501 cacataaaac caggaattgt ttatcttcta cttcagttaa ccgctgtttt aatccttctt
     4561 ggccaaaaat attcatccat cctctaaaat aggcaaaatg agcgtgattt tgcatcactg
     4621 tcaacgcaat cttttgcgaa atctcttctg gaaaaacata aaaagagaaa tattcttcta
     4681 tcgattgttt aaacgttcga ttcaattgag ctactacgtc gtctttgtat ttttcgctta
     4741 tcaaaaactc ctggacatgc gagttatgtg tatctaatgt aaagttactg atcatgaaaa
     4801 gataaaaaac caacgtttca ttttgattcc aaggaaaggc atattgaaag aagtaacggg
     4861 tcaaaaatgt atggaggtcc aataaaaatt gtgtcgtttg aaacaatcca acaggaagat
     4921 tgctataatc tggtttggat tttttcgtac agcgcattcg cttcttcata atggaaagcc
     4981 atatttcaaa tttcaatcgt ccttcttctg taatagcaaa actaaactct ttttgcagca
     5041 gatctaaaaa agtgtttcct gataaatcac tgtattcttt tttgttttca agatagggac
     5101 gtccaaacca aaataattgg aaataaaaat aacgaatttg actttctgga ccggtcagcc
     5161 gcccgctttt tattttcaga tgaaactcat ttaaagaatc atttaattcc ttcactctgc
     5221 gagacaaagt agccgaactg atattaagtt ccatgacaag tttctgccga tcaaatgagc
     5281 cattttccaa cagataaaga agtatctgat attttgtaga atagcgcaag taacaatgat
     5341 aataaacatc gactgctgct atatttccat gtctgattaa taaaatattc ccttcttgga
     5401 taacaatttc caattgtttg atattttcca agttagcttg taaataaaac agatcttctt
     5461 tcaaggtagc ccgatcaact gataagcggg taagtacgtt attaacggaa acctctcctt
     5521 tttcctctaa taaaagttgg aaaagtttga tttgccgttg ttcatttttt tcaagcaaac
     5581 tgaaaagttc caagtccact ccttcaagca tttctgattt taacagcaga agtttccttc
     5641 tcatccttga aaaagaggat ctgtgacagt tatcacagat ccatacaaat tctaatcact
     5701 tagctggttg gcccagctgt cagttcccat gtgaccatgc cttgatacgt tgcgccaggt
     5761 tgagcattcg tactgctatt tacctctaag agtaaaccat cggtatcggg aaagtcaacg
     5821 agaacctctc ctttagctgt tccatcactc tcataaatca acgtgttttg accttgctga
     5881 atcaattgct ttgatccaga atgattgaag tataattttg ttccgcttaa ttcttgacct
     5941 tgatcattct taaataaaga agtcaatgta cctgtcagtt gccaaggaga cgtgcttgga
     6001 agccgagtat cccgcacatc aaaagaaaaa tcgccttttg attcataata acttgtcttc
     6061 aacggtttgg ggagatcgtt accaaatgta aaatcatctg gtacattaac taatgttaat
     6121 tcttgtttgg ggacaagaat tgttttagca gatgtttgga caactgggtt aggaagctta
     6181 gcaccatctg ctaatgtaat attgctggaa agtacagctg cctttgtcag tatgctgacg
     6241 gattgtaaag aagtatctac agtatttaat tgatagttgt aagtaagatg aatggtatct
     6301 cctattttgc tcaatagatc tgctgggaca gaaactcctt gttgaatgcc ggtatctgcg
     6361 atagttgtac catttaattt taaggaacca ttgatataag tagtattttc aggaagtgag
     6421 atcgtgattt ttttgtcggt tatcgcatcc ccagattttg ttaacgtgaa tgtcacattt
     6481 tcttgaatcg tatctcctga tagagcagac aggggatctc ctgtctcagg ttgtctgcta
     6541 aggtttttta gctgactatc tgcactcaca gatggaaaat tagccactgt tatttgagga
     6601 agagtggtga ttccagcttg ttcagactgc catttgtacg caaaagacgg atacatgtta
     6661 gtaagtacat cgtccaccaa gcgataatca aaataatatt gccctgaagc caatgcaggt
     6721 agtgtagaag tagctgtatt tacacccccg ttatttgatt ggatcgtttg gaaaagttga
     6781 tctgtccatc ctgtcgctaa tttttgggtt cctttcgaat aaagcctgta atggatcgaa
     6841 ccctttttta gttcagttgc tgaccaattg gccgtgactt ggtacgatgc agaagaaagg
     6901 agctttcccc actgactatc ggctgttaag tttgttggta aaaccgttcc gtctagcttg
     6961 tactgctcca atacctttaa agcaacagga gaaagcaatc cgccttgcgc atctttccca
     7021 ctgtacacga tattggctgt ctgattcaat ccacttgtgc ttgctggagt atgagtcagt
     7081 attgctcctt gtgtaacgtt ttttcctgta aaatagttga ctacgcgatc aaagcgacta
     7141 aatggcaact gatcaaaacg tccttcagtc agccccatcg tcaatgtccg actattggta
     7201 gagcctggaa ggttctggtt atttccatcc acgtattctg tggtatcagc aaatgtatta
     7261 tattttcctg ttgaatcact tggtgagatg taactattcc acgtatttat ctctgcactt
     7321 gcagaaaatg catatgctcc tggattcaag tttccgccag ttaatttagg ataaaatcta
     7381 cctgcagctg gagctaaaat cgaattgaac atacgatctt ggataatatt attcgaatca
     7441 taaaaatcaa cagaaaaaac ggccttttta gacatagaag gttgtaattt acccttggtc
     7501 aaaattttgt ttggactcgt atcatattta tctccgtttt ctgtcatctt cgcgacatta
     7561 gctgtaacat caaattgaag cgggatatcg tccacgttga attcgcctcg cgtaataata
     7621 gcaataatat ctgtcaatag agtatttgta tcccacgacc ccatcccaaa acgcaaggta
     7681 tggcttcctg ctgataataa acttccgcta gtaaccttga tgggggcacc ggcaatatac
     7741 aagtaggcgt tgttccagtc aattgctttt aatacatcac tgcttgatac atgttccggc
     7801 aaattgatga tcgccacaaa tgcaaatcct tgtgcagtga tattcaccaa atcaagtaca
     7861 tttgttagtt tcacatggct ttcccaatga aaacggaccg tgccaccttt ccaaggatca
     7921 tttgaaccat cgttcaaata agtagcagtc aatttaggca attggttagc cccagttagt
     7981 tggacaagct tcccgccatt gactgcacta cgtccaccat ttggaggtac gatattttca
     8041 tacgtgtatg gatcataaga aatggcagga ggcgttgctc caacagcttc tgccgaaggt
     8101 gaaaagataa atatacttaa aacaaaaagc gaagtaaaaa agaaggagcg aaaaaatgaa
     8161 aatttatttt gtagcaacat gttcatcctc cttttccttt cttctccata acaacaacca
     8221 cgcaagaagc cataattcta taccaatgat caaataaaga aaacggatct tttgttcatt
     8281 cgtctccggg aatacgccat gttgattaga ttctttataa atcggtagct gtgtctgctg
     8341 ttttgatgcg ccattgactg aaccacttgg atgcggaatt ggacttagat cataaaatgt
     8401 aatcgtacct gaacttttgg tatctgcttg gactggctct tgccagctga agaacagcaa
     8461 acttgcaata atccctccca ataagatcga tgttttcaac gaaatcacct catcatgttg
     8521 tttctccttt tttctttttc ttgtggaccc acaaaaacag caaaatcaac cataacacta
     8581 gaactgcagc aaccaatcct gcataaagcc atgggaaaga ttgttcttgt actgcaaaaa
     8641 cggaattttc atttactttt tgtgcttttt cttttttgat cgtaaagttt tgtgtaaatc
     8701 gccattcatt actgtcgcct gattttgcta tgatttctgc gcggtaatcg ccggcttttg
     8761 ctttatcacc agctaaatca agattcaagt gaaaattcga atttggtgca actaaaaatg
     8821 cattagacgt attttcaata taaagctgat tttcataaaa gatcttgctc gtaaatatca
     8881 aattgctgat aatcctaggc tgaggatttt ggaaacgaag ctgaacagtt ggctttgcgt
     8941 tgacctcgtc caaatctcct gataaaagtt tcagatccgg ttctatcgtc tcttgacttt
     9001 cttgtaaaac gactgcgaga ttataagcaa tacgattttt gatttgtgct ccttctgtag
     9061 cattttctgt ttcagcttct tctttaggtg aaacatgaat acccgctaat acacgtccct
     9121 taaagctttt tgctgggatt ttcacttcta taggtacagt tgtttctgca tgtgctgata
     9181 cattgatttc ctttttcgca atgagcaaaa cgtcacggac atcaaacggc aatgtctcat
     9241 ctaacgttgg gtttttttga ctataatcga tcgttccacc atcactcgtt accgccggat
     9301 tgacagagac ctcaaatgtt cgttcttcat ctgaagaatt gctgataatt acttctagtg
     9361 tctgattttg ttccggtgcc actaataaat caaaataacc gattgcttta tctatttggt
     9421 tctctggaaa taccggtttg attccaaagt ctcctgctga atcctttgct tggctgattg
     9481 acggaaaaat gaaaaataga cttaaaatag aaaaaaacga aacaaacagt cgtttacttt
     9541 gattcattgt ccaccacatc ctttcaacaa aaactgcggt aaaaaatcct tcacctatag
     9601 tattttcacc gcagtttgtt gaatttattt catactctat gctactgggc cagccacgag
     9661 gttccaagtc aaatcagccg tgaatgttcc agctgctgct ttcggcactt tcagtgtgat
     9721 atttgctgga tcataaacag aaagccatgt tcccagacct tgatcttttg ctgctgtaaa
     9781 tattggtgtc gctgcacttg ctttactcaa cccagttaca gtttgactag ttggtccagt
     9841 ggattcgtta tctgacgtac ttttaacagt ggatgttcca aaatctaatt cagcgccttg
     9901 caaaacttgt gaaccgttta caaaatctga aaccgaaact gtcacgttcc acccttgtgc
     9961 ttggccatct gcacctacac ctcgacgatc tgatacttgg agataaggtg tatcgttttt
    10021 tgaagcgtct acttgatacg tttttgttcc gctttcaatc tcgtgtgtac caaatggcaa
    10081 ttcgggaact acatccagtg ttaaaggacc tgtctggcca gttgctggat tttccggatc
    10141 tgctggctct actggttttg aaggatcagt agggttaaga ggctccacgg tatttgttgg
    10201 ttcagtaaag gtaatatctc cttttgaagt cgcttgaccc tcagcaaaag ctggtgttgc
    10261 caagccgcct agaagaagtg ttgaaagtaa taatgtccca atcatgttct ttttcataat
    10321 aaatcactcc tttttcttag tgacctaagt atagtagaga cagtatgcaa aaaatcctca
    10381 tgaattcatc cgcttacttg aaattatgac cctttttgta taaaagagaa aaaaacaata
    10441 aaatcgttta catgaatatt cttccttttt tattaagtat gaaaaaatat ttcaatttta
    10501 ttttttcgtt tcaaattatt ctattagtga catccttgaa ctataaaaat aggagtacgg
    10561 catcatctgc cgcactccta tttttttcgt catttgtttc ttaaataaaa cgattctcac
    10621 gcaattttat ttttttgata tcttctactg tttgtgttcc agctaattgc atgaccattt
    10681 ccaattctgt tttaaagaaa tcaaacactt gatggactcc tgtacttcct cctaaagaca
    10741 atccataaat aacgggtctg ccaatagcaa caagatctgc acctgatgcg attgctttga
    10801 aaacatgctg tccgcgtcgt acaccgctgt caaacacgat tggtacacgt ttatctactg
    10861 cttctgccac atattgcaac gaatcaaacg ctgctggacc accatctagt tgacggccac
    10921 catgattaga tacccagatt ccgccagcac cagattctaa tgaacgatag acatcttctt
    10981 ctgactggac acctttcaca tagactggca aatcagaatg agcagcaata aattctacat
    11041 cttttggact aagcttctgt ttggaagatt tgtacacagc atccatcgtt tggcctactc
    11101 ctgattgata agcctgaacg atcggcattg gcagtgggaa ggtaaaccca tttctgcggt
    11161 ctgtttcacg gtttccgcca actgttgcat ccgctgtcaa tacgattgct tttgctccat
    11221 tacgcttggc cacttcaaga atatccaaat tgatcccatc atctttgctc atataaaatt
    11281 gaaaccattg cggtgctttc tccccgcctg cttctcttat ctcttctaat gtacaagaag
    11341 cgtaagaact tgctgtatag atcgtcccaa aatctgctac accttttgca gaagctttct
    11401 ccgccttcac atgagctaag ccatgagcag caactggtgc catgatgatt ggtgcagtca
    11461 gcgtttcttt gtcaaaatgt gtagttgtat cgggtaattc tacatctttt aatacatgag
    11521 gaataatcag ctgatgattg aatgcccgtt cattttcttg ataagtaaat aagtcgccag
    11581 caccactgct gatgtaacca taaccaccag ctggaatcac ttgtgaagcg gctatttcta
    11641 aatcttccat attgataaaa tccactattc cttcatttgt acctgcttga tacgtttttt
    11701 ccatttcaat gccttccctc tataaaaaat tgaatgaata atatgatagc gtttacacac
    11761 tttatattac tcttccgtat tgatacaggc aaataatctg tttacatttc agtcattcca
    11821 gttgttacta ctctgaaaga aatgtttaga acacaaatag ataaaaaacg atgggacttt
    11881 gacaaaagtc gtgtcaaagt cccttttacg aaatactgtg gatgcctatt gaaataagtg
    11941 cgggttgttt ctttgtattt caatcgtttc ttgccattct tcgtttaatt ttggattacc
    12001 gttttcttcc ttgaacgcat aaggtcccaa tccaaaaagc tcagttagta tttgattcac
    12061 gattccatat agctcggtgt gatcggacac atgattattg aagatcatct cattatcacc
    12121 acgtttgaat ctcacattcg ggctctcatg atgaacctct atgcagtttt cccctttttt
    12181 taactgacgg atttctgaaa acatctcata aatatcttct ccaataaaac cttcagcaag
    12241 cgccagactg tccacaagac cgcctactgt caactcagag attatcgctt tgttggcacc
    12301 aagctttaat gtatagcttg ttgcttctac tacgccgcat gcattgcaac caccttctga
    12361 tagttcgctt tttctgatca attgaacttc tttcattttc tgttcctcct cttagttttc
    12421 tacttatgta ttttttatca cctgacgtaa gtgtatttta tcgtcacgat cacgtgattt
    12481 attcttcaga gattctattt atctatctaa catatccttt attcttctac ttttctttct
    12541 ttagcatata agagatacgc atagtaatca agcaaggatt gttgatataa aaagaacaaa
    12601 taccgctatt ttgatttctt ttgactctat tactcatata aataaaattt attatacctg
    12661 ctctttttga ctatatcctc atctgactcc tatacttcgt ttgtattaaa acttaggagg
    12721 aatcattcat gcttacaggg ctaaacttat taaataatcc ttttctaaat aaaggaacag
    12781 cttttacaaa agaagaacgg gcaaaatacg gaatcactgg tatgcttcca agtactgtcc
    12841 aaacattgga acaacaatct gtccaagctt acggtcaata cttaagcaaa cagaccgatt
    12901 tggaaaaacg tatcttctta atgaatctat tcaatacgaa ccggacattg ttttataaat
    12961 tgatgggcga acatttagtt gaattcatgc ctgtcgttta tgatcctgtc gttgcggatt
    13021 caatcgaaca atacaacgaa attttcttga aaccacaaga tgctgcattt ctttcaatcg
    13081 acgaaccaga aaatatcaaa gcaagtttga aaaacgcagc agatggacgc gacattcgtt
    13141 tgatcgttgt gacagatgca gaaggaatct taggtatggg tgactggggt gtgaacggtg
    13201 ttgacatcgc tatcggtaaa ttgatggtct acactgctgc ggctggtatc aatcctgcac
    13261 aagtcttgcc tgtatcaatc gacgctggta ctaataacga agaattattg aataatccat
    13321 tatacttagg gaatcgtcat gctcgtgtag aaggcgaaac ttactacgaa ttcattgatc
    13381 aattcgttca atcagctact gaattattcc cagaattgtt attacattgg gaagattttg
    13441 gacgaggaaa tgctgcaacg atcttagaaa aatacgaaga taaaatcact actttcaatg
    13501 atgatatcca aggcacagga atcgttgtac tagctggtgt attaggtgga ttgaatatat
    13561 caggtgagca attgaaagat caaacgatct tgacatttgg tgctggtact gctggtgtag
    13621 gtatcgcaaa catcctttta gatgaaatga tccgtcaagg tgtacctgaa gaagaagcac
    13681 gccaacattt ctatcaagta gataaacagg gtttattgtt tgaagataca aaaggattga
    13741 ctcctggtca aatcccattc gctcgtaaac gctcagaatt cactaatgct gatgaattga
    13801 caaacttaga agcagttgta aaagcaatcc atccaacgat catgatcggt acttctaccc
    13861 aacctggagc attcactgaa gaaatcgtga aagaaatggc ggctcatacc ccacgtccaa
    13921 tcatcatgcc tttgtctaat ccaacaaaat tggcagaagc aaaagcaaaa gatttgattg
    13981 aatggacgga cggaaaagct ttagttggaa caggtatccc tgctgctgat gtggaatata
    14041 acggtgtaac ttaccaaatc ggtcaagcaa ataatgcgtt gatgtaccca ggtttagggc
    14101 ttggtttgat tgcttcaaca gctacacgag taaatgctga aatcatttct caagctagtc
    14161 gagcattagg cggaatcgtt gatgtaacaa aaccaggagc agctatcttg cctccagtag
    14221 caaaaatcac tgaattctct caaacgattg ctgaaactgt cgcaaaatca gttgttgcac
    14281 aaaaattgaa tcgtgaagaa atcacggata tcaaagaagc tgttgaatct gctaaatggg
    14341 ttcctgaata taaatcttta gaagattaag attgttatag taaaagaaca tacagaaact
    14401 ttgtatgacg tctaaaaaga aaaaagcgac tgcggcaaaa cagcagccag ccaactactg
    14461 ctcttttgcc gcttctcttt ggcttttttc atacgataca gaataagaaa ggatctgtaa
    14521 aaatggcaca aaaaacaaca accgcaactg cagttgagga aaagaaaaaa gaagggtttt
    14581 ggtcaacaaa aattaccggc gtaagtttgc ccctttattt gatcatggtc cttgtcctga
    14641 tcattgcaat ggctttagga aaattaccga cgaatatgat tggcccaatc gctgttttgg
    14701 tcattttcgg taatttattt cattttatcg gaacaaaaat ccctatcgtc aaaagttatt
    14761 taggtggtgg ctccgttttt gcgatctttg cctctgccgc gattgctaca tttgggatct
    14821 tacctgagta tgtagtaaaa tccacagaaa actttgtcag caatatgggc tttttagact
    14881 tttatattgc tgccttgatt actggtagca tccttggtat gaaccgtaat ctattgatga
    14941 aagcttctgt tcgttttatt cccgttgcac ttatttcaat ggtcgtctcc ttttttgccg
    15001 taggactcgt aggtatgctt atcggaaatg gttttgcaaa ttccgtttta tatatctcat
    15061 tgcccgcaat ggctggtggt gttggagccg gagctgtacc tttatcaacg atttatgcaa
    15121 atgttttagg tacggacgct tcttctatca tttcccgcct gattcctgcg acagcaatga
    15181 caaatgtcct agctatcatt ggtgcagcgt tagttgctcg tgcgggacaa tcaatgccaa
    15241 aattcaacgg taatggtaaa ttaatggaaa aaggcgacct tggcgatgga aaacctagaa
    15301 atgtaaaacc ttccattcca caattaggtg tcggtttgat ggtatcattg actttcttca
    15361 tcttaggaca aatctgtaac tattttgttc ctaaagtcca tgcttacgca ttcatgatca
    15421 ttatcgttgt tatctgtaaa atcgcaaacc tattaccaga atattacgaa gatgcagcta
    15481 tcatgttcaa caacttgatt gtcaaaaact tgacagcagc tgttcttgcc ggtatcggta
    15541 ttgcgttatt gaatctaaat gtattggctt ctgctttgac atggcaattt gttgtcttat
    15601 gtttgacaag tgttatcgcg atttcagtag tttcaggatt tgtcggtcgt ttgtttggtt
    15661 tatatccaat cgaatcaact attactgctg gcttatgcaa caactcgatg ggtggtacag
    15721 gaaatgtagc tgtcttatct gcagctaatc ggatggaact tattgctttt gctcaaatgg
    15781 gcaatcggct tggtggtgcc atcgttttga tcatctcagg tttcttgatg caattacttt
    15841 cttagtcaaa aaaaatacct gatcatcaag tatcggcttg atgaccaggt attttttgat
    15901 aaaaatttac aggattttca aaaatctttt ttgcatttta tcggaatgat aaccgatgcg
    15961 ttcataaaat cggtgtgctt ctactctgta ttctgcagaa ttaagtctta ttgctgatat
    16021 tcctcgtctt ttcgcttctt ctacgccagt catcaaggcc tttccgattc ctcttttttc
    16081 agcatgttta gacacagcaa gtgccataat attgaacatc ggatcgctga aaatcgattc
    16141 ataggactcc gcttgtacat agccagctgc ttttttagaa gtaggatctt catagaccaa
    16201 gaaaaaattt ttttctggct ccttcaacag tttcttcatt tgtctcaccg taacggttaa
    16261 tggtacatca tatcctaatt gttccgcatt gatttctcgt agtgcttctg catctcctaa
    16321 acgcatttcc cgcaacatcc aaatcaccag ccaattttta tttttttaaa tgaatttttt
    16381 tatcacagta tactcctttt ttcatttaga acaatatgtt tcagtgattt tgacttgcgt
    16441 tagtctagat tctcctaatg tttgtaagat gacgaaagca ttgtaaagat tgatcaaaag
    16501 aaccacgagg acaattactt gaaaaataaa ggtcactttt tttggagaca aaaccctgct
    16561 aaatcttgct ccccacaaac ctccaataac agcagcgggg ataacataaa acaacataga
    16621 taaatcaaag cgcataaagc tacttgttaa agcaatcgtt accaattttg ccagttgaga
    16681 aaagaaaatc gtagatagag aatatagtgt tgcttctttt attggcaatg caaacatcag
    16741 catcaacaaa gaaacattga ctggtccacc accaatacct aaaaaactag ctaaaaagcc
    16801 cagaaccccc ccacatataa ggtaccaagt ccatgacgtc aaatgaaatt ttggctgatg
    16861 atgcttggtg tagaagaacg caaaaaccaa ggtcaatacc gtcaaaaaaa tttggatcag
    16921 ctgcacatgc ttctcgtttt caaataactg taaaaaaact tcgaatacaa tgttgccgac
    16981 tactcctcca acaacagctc cactagatac ccaaccgaca atctgccaat tcagtgattt
    17041 cccgcttgat acttgtcgca cagtcgaaac aatggacatc gtgaataccg caactgttga
    17101 gtaaaaagaa atccctgcaa cagaatgggc gccgataaga tcaagaatcg gtttgatcaa
    17161 cacgcctcca cccatcccgg aaatagcgcc aactgtgttc gccagaacga tcaccacaaa
    17221 ataaatcaga ccaatcatct tgtatttcct ttctcttgtt tatatctttt agcgattgtt
    17281 actcttttct tttattatac agtaaaagaa acaaacgagg cttcgaaagc ttttctactt
    17341 tttaaggaaa tttagtgcta aacgaaaaga ggttgtgaaa aagctgtcct gcatcaagta
    17401 ataagaacag ccaaacaaaa atagctcctc ataattttcg tttggttgaa cttattaccg
    17461 cagatgcaag cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg ttcagctccg
    17521 agtcacaagg aacaatttca aaaaacagct tctcatgttt gttgaatatt gtgacttgtg
    17581 accgaggagt tggcaggaga acaccgttta aggaagagac tgtgaagtca acgttttgac
    17641 ctgattatta gagaaacaaa tggcaaaata gttcttcata tttcgccgtt tttcgagcga
    17701 atgccgaggg gcagctgatt gaacatcgtt tatttggaaa aagggattgt gacaagacgt
    17761 ttgtcacaat cccttttttt atatcttatt aattcattct tttgttctat tcttcttctt
    17821 ccgccatgaa tgagaaaccc atatcaaaga actataaagg ttatcaaaac agcgtttatg
    17881 actaaatgaa ttagagttat ctatcactat ttttcaagcg atttaaacca aatggcatac
    17941 aatatggcat acaaaaactt caataagccg tgggggtttt taaatttctt cttgttcttt
    18001 aaaatcaatg agtggagcta cataatttga atccactttt tcaaacgtca ctttttgcat
    18061 cgacgaatgt tttctttccc aagataacgt aaatagattt gactctggac ttataaagtc
    18121 tacttcacct tctagtatta ctttcttatg caaatattcc tctaataaat atagacttgc
    18181 taaagcatac aacacatttt ccaaatttgc ttccttataa tttgaagaac gaccatgttt
    18241 tactctgtta tatattttcc accaatctgg tgtatcttca ggccacccat ctccaaaagg
    18301 ttttagtgaa gtcaatgtta aagaattcat aaccctaaca ccattatcca aacacattaa
    18361 ttcgctagat ttaataacag ctttaaaatc agttatgctt ttattgctac tgctatcaaa
    18421 tccgcaaagt tctttgaaaa taatctcaca ctccgaacca atcgctagta gtagtttttg
    18481 atattcaata gagaatgttt tgaaattatt ctcatcgagc atcacaaaat ctgtggtatt
    18541 aataaactca ttttctaaat gtagataata cttccaataa aaatttgtaa actctgcttt
    18601 gtccattaaa tcacctcttt tgaattataa caaaaaaaac acgtccacaa cagacgtgtt
    18661 ctttgatttc tacacaatca gcttgccaaa accatccaat cagtcctagc atgcctgtat
    18721 taaaattgaa cgttcatcat ctatcatctt tcttagtcca gcatgtttgc aaccacacct
    18781 acaattttca gtccaacaat agatttcgac tagctaagtc ttgcaaaatt caggcagcta
    18841 acccttcatt tactacctta attatactaa atacccacaa cgtttacaag tgtaatcatt
    18901 ctaagaaatc tgtttagcta tgatcgttgt ataatcttta aacgccgttc cttcattgat
    18961 tgaaataatt ttataactct tgtttttcca agctactttc atatcgtttg ttagttcata
    19021 atcttgatcg tatcgaataa taaacgtcaa cgttccttca agaatggtac ctacactagc
    19081 aagcacgtct tttagcattt gtgaacgcac attagcccaa cattcaaata cagtttcttc
    19141 actatccacc atctgcccat cttcatcttt tacccgtttt gtctttttaa acttgactct
    19201 ctcagtcaat tcatttacat tcttaatgat tggcataata agcacctctc atttgttgta
    19261 ctaatgcttg taccgttact ggtatccgtt cgctgctggc ttcttgacgg ttcaaatacc
    19321 aatgttgagt aagtaatgac accgccaaat caaattgctg ataattatcc atatctttat
    19381 ccgttgcgct actatcaata gcacttttta tatattcgcc tgctgtatca atcaattgtt
    19441 gaataaagtc atcatcaata gtgtgatcga tccgcatact tttttttatg ctatccaaaa
    19501 ttaccatttt atctctccta atgaaaaaaa ggcagtggga taaaccgccg cccaatattt
    19561 ttctatactt ttggtgttac aactggcgta aactcaatat acactgaagc attttgatca
    19621 attttcttat aatcgttgcg aacgatcact gcaagtcctt gtgagtagta atcgaatttt
    19681 tcccattgag tcgttacttg atttctacga gcaacaaaga tagattgtgc aatatccccc
    19741 ataatcatag ggaatgttcc agctttagga ttggcaaaca atgtatctgc aatcaatact
    19801 actggcatac cgaataatga tttaccacta ggtgctgtca catcgggttg taaaatgtaa
    19861 cgtccatctg aatctttcaa tgtatctagg tggtaatatc cgctttggtt tagaattacc
    19921 attttattta atgctgggtc taatgccaca ttgtatagtt gtttcaaatc gtccaacgta
    19981 gcagccgttt tcttagtgaa tgtttttaat aaatccatga tatgcttatt gtcagtgttt
    20041 tctaccaatt tcgctaactg atctttgacc tcttgtacaa tattcactgc tgaatcttcc
    20101 acaacctcat ttgataaggc aatcttacca gcacgagttt ctactttata atcaactgaa
    20161 gtaaacattt cagcgtcaat atcaccaatt tcagcaagtt cagcttttgt tgctaacact
    20221 gcttgttggt tagttgctac tggatattta ccttgtccat ttgatactgt ttttactgtt
    20281 gcatattgag ctaagttata atttgaacgt ttcaaatcaa agacttcacc gattacttct
    20341 tcgggaacaa ctgccgctgc atttacagta gtgactccat cacgcacttc gccttgtgaa
    20401 cggatatatt cttcataccc acgcaattct gttttttcgt tatcgattaa agttttttcc
    20461 atgttattac tctccttttg tgctttagaa gtggattcta agctattgtt tgtttgtttt
    20521 gcttggttgt tactcataaa cgattcgtaa gaacgcttat ttacttggac atttgatgaa
    20581 tcataagcgg gaacggtcac gacactaatt tcattaagtg ctttgatttt atctattgat
    20641 cgttcaattg tgccatcttc ttttttgtcg aatgaatcta atccaagcac gaacccaaag
    20701 gacatagaat caacaacccc ttttgagata ttctcataca catcatttga ataggtcgtg
    20761 tcggttagct ctgcttcaaa atgtaatcct gtatcatcga cattcaattt caacgtacct
    20821 gctttgacac tagctagtgg ctttgaataa tcgtgtccat ataataagaa cacatttgat
    20881 aaatccacct cttttaatgc ttcgggtgta atcacttcta caaagccgcc taaatctttt
    20941 gacggttgcc cgaatttcaa cgcatagcct gaaatagttt tgccgtctgt tggttgtttt
    21001 tcattttctt tctctacttt gggagaatcg gctgttagct cagcttcttc tgtcagccgt
    21061 ttttcctttt cttcttcatt attcaactgg tgtaactcct tcctgttctt ttaattgata
    21121 tttctccaac gtatctaaat acgtaaagtt caaacttgct agtaaacgat cgccaccatc
    21181 catggggggt agccccattt tcgaccgccc ctcattaatc gtgagaagtg accctttgac
    21241 ctgatccaat acatttttta cttttgtttc aggatcagtc tctagtaact gatcggaatt
    21301 aaagcggata ttggtttcaa gtttcgtatc taattcactc acaaacacat taaagtaatg
    21361 aatcagcgta ctttggatat actggagatt actttgaacg gtgctagaat gttcattttc
    21421 aacgcctaaa cgctcaatcg gtacaccaaa cgccttggca atttgttttg tgttccaatc
    21481 attggaattg ataagcttca aaacatcggt gtttatttct aatgttttgt agtccattgt
    21541 ttcatcaaga atgatcgttc taagagcatt atctccacta cttgaaccgt tggcttcttc
    21601 aaacttttca cgaattgcgt tctttgctga tccgtctaaa tctgacttat gaaccttcaa
    21661 aatacctgac ccactgacac cacgagtaaa gaaattgtgc aatgtgcggt tgccagcttg
    21721 ttgaattttc agttcatcac gtaacgcata aagtggtggt aatcctgtta aaccatcttg
    21781 ggtgaaatat ttgaagtgca aaatatcgct agacttcaca cgtctttttt tatcaccaat
    21841 ctgataagac aacgtaccat tatctaattg agtaaccgtt acacttgagt taggtaataa
    21901 gtgaatttct tctacctttt caccgttgcg tttaatctct gcaaagctat taccgtttag
    21961 caacatattg accgccaaag caaactttaa atgccaacca tccatttctg aattaggttt
    22021 ctcgtttagt aacttcacca gttcattatc agcttgtggc atattgtttt tgaccaattg
    22081 aatcggactt gaagcaatat cactagcaat aattcgaacg gctgcaaaca catcactatt
    22141 tcttaatgca cgaacgcttg taaagctggt ggtgtaatca tccgattgga ttgataccac
    22201 atgttgtagg aaaggatctc ccgttgtatt ttgtctcgct ttaaaaaatg ccatctttta
    22261 acctcccttc tgttctgtat ttagcatgac cgctacccca ataagcacta tgcccagcgc
    22321 taacaagcca tagtagacat ttgttagtaa ggtgatagca accaacacac acaccaatcc
    22381 aagaagtaaa agaacggtat gaatgtaatt agaaagaaaa ttcttcactt gtataaaatt
    22441 cattgtctgc tttcgctcct tccgttgtgg tgaagtaatc cattgcaaac acataggcat
    22501 tgattaatgc agctattgga tcaattttgt tactgttctt tgatttatta atctgaatgc
    22561 cgttgttatc cgtttttaaa atagcgttgt tcactgcatg ggtgagaatc gtatttttat
    22621 tgtgaataac gttgccttca taaacttgtt ctctaaaagt tcgggtcgga acgtttagag
    22681 taatcgttcc ttgtcttact tctaacattg ggtagttggc tttttcagct ttactgataa
    22741 gcgaattagc gttatacggg tcataacaaa tccccataca ttccaaatca ttttcttgaa
    22801 tcaaatcttg aataaactga aatacttggt catagtccac gattccactt tctaattgag
    22861 taatggaaca ctcaccagct ttttctagtt ctcgataagg caaaccgtcg cgcttttctt
    22921 tgtcttgaag cccatatttg gtggctacaa agctatgtga atcacaataa agttcgccgt
    22981 tatctagcgg tacgatccat gaaacgctag ttaaatcatc tgttttagat aaatccactc
    23041 cgatataaac gggcttgcct gtaatatctt gtggttctac ttcggttgcc tgccagtctt
    23101 cagcaatcat atagctgtcc tcactcgctt gtctccataa attgaagttt ttcaccaata
    23161 cagaattaag gttattttgt ttcaaagcaa ggttcacatc atcttgaatg gttggaatca
    23221 ttactttttt gatttcttca ctttcaaaaa tcggattggc tttgatccat ttctcttgat
    23281 cgtgaatttc ttcgggatcg tccaattccc atatcgctat aaagtacctg tcggcttgtt
    23341 cttcgccttt tagcacacga tctaacaata agtattcctc gtacatagga acgtttaaat
    23401 caagcccagc ggtactaata acacacaata agccgttctt ttgttgggtc attcctgact
    23461 taatgacgtt gtacgtttta cgagtttttg cttcgtgcca ctcgtcaaga attgcggttg
    23521 ttggtgcaaa cccatctagc gtgctggttt cactagccag tgccatagcg aacgaattag
    23581 agggcaagtg ctgaatctta gagttcataa ttttcagttg tggtcttaaa aacttactag
    23641 acttgaccac gtttctaagt gaattagaca gcatatcata gcccaattta gcttgtttca
    23701 aagcgttact tacaaacaat acttgccttg cttctgctgg ttcttgttcc ataatcaacg
    23761 cattggcagc catgccactt gcgatatacg ttttcccatt cttacggctc atactggtaa
    23821 acgctcgatt gaatcgcctg taattccctt ctttagtacg ccagccataa agactgccta
    23881 gaatgaattt ttgaaatcct aacatcttga ttgctttacc gtccgtagat ggtagcaact
    23941 caatgaattt aatcgctttg gtggcttgtt cttcatcgaa cacataaggg aagctatctg
    24001 acttagaacg ctccaaatcg cttatatgac gctcacacgc ttggataatt ttcttgggtg
    24061 caatcagctt acctgatagg actttctcaa tgtaatcatt catgaattca tcaactcttt
    24121 gaacgggtct tcgggttcat catttttaac cttgttcaaa gctagttttg atcgtgattc
    24181 taaggtcata cctagtgagg tggcttgttt tttcaaatca ctcatagctt gtgattgaac
    24241 ccgaaccgct ggattggctt tctttactcc gttttcgcat gtgataaacg tacctgattt
    24301 tcttatctcg gcttcggctg ttttcattcg agcaaaggct aaacaatagg ctaccaatga
    24361 accataatct gcttcactca atggataatc tttttttaat gttggtacga tacgattcca
    24421 ttcacttcta gcgctcgctg ccatccagtc agggggcgtg atatttatta atggttgctg
    24481 cttgaacaat tcttctctag cgtccacacg gattgctttt tcttcacttg atatattgcc
    24541 ttttgtatcg tgtaatattt tagggtgtcc catgttgtcc tcctttcaga caaaagaaaa
    24601 aggaacgtaa gcaattaagc tcacattcct tcgtttttcg atcagaattt ttctttatct
    24661 aatttatcta tataaatgat ttttccgttc tgcatttgaa tggtcacttg accaaaatga
    24721 ggtaactcaa tggtgcgctg tttccctgtt ttatcagtaa taacaattgt atttttcaat
    24781 aaatctccta ctttcattat atactttttc acttgtgatt acaagtgtaa tcgttatttg
    24841 taattaccct tatgcctttt cacaacggtt atttgtacca aaatgagtag gcatcgattg
    24901 tcagtaagtt tcacactatg cccccttaac tcaaagggta ctcttttctc gtctttgtct
    24961 ttcattggct gtcttggaat tatggtgtgc atggcataaa gaacgaaaat tagtttcatc
    25021 taaccgtcta ttccaatcat cttttagttc aattttgtga tctacaacat cagctttcct
    25081 tatcaagcct tcttctaggc aatcctcaca tactggatga tttaaccgat acaagtatga
    25141 agcctttcgc catgttctac tttgatagaa ttgaaaatat ttaccttcgc tttgctttct
    25201 ttcttcacgc ttaattgccg tttgctttgc tttatgcttc ccacaatact tctgcctata
    25261 atcaatcaat gttttacatc cagcatgatt acattgtttt ttaactgcca tagattacca
    25321 accaaagcga tctcgttcgt cagaatcatc ttgttcccat tttgatttgt attcaatata
    25381 aggcgaaact atcttcaagc acgttccgtc tttaaaatta actttagtga ttttatcttc
    25441 ctcaaaacta ataaagtcta catcttcttg agtgtttgaa tactcaatgt gtttctcacc
    25501 atcgaagtat ctaatacttt ccatatgcat aattattcta cctccaataa attagttatc
    25561 aattcatcat ctgttagtag caaagccaag aacttatcat ggatgtttaa aaacaaatta
    25621 tcctcattcg atgtaagctc tcgttgtctc gctcgatcat ataggttaag cagacaatta
    25681 aattcatcca ttagctcacc tgtatcatca tagttgccac tatctaccaa tgatcccact
    25741 actagcgatt gacaccacgc tactgactcc tgcagccaat caggttcttc ttccattatc
    25801 tttaatactt ctggatcaaa tgtggaataa tcttctgtta ccaatcccac actaaatgcc
    25861 agcgtgttca ttagaaaccg atccatttca tctgtatatt gaaagttcgg tgctttgttt
    25921 gttgaaataa tattgagcat ttactttcct ccttgattcg taacacgtgg taacacctac
    25981 gtaacaggta cccgttacca ctcaatccca acggggacaa gggttctagc gttttggtaa
    26041 caggtaacgg gtaaaatcca gtaacattat tccccagcat attagctaac tatttcaaaa
    26101 gttttaagga aataaggtgt taccccgtta ccaaattgct gtatcccttg gggcagtaag
    26161 ggtaaactag taacgggcta ggtgttactg acccgttact acccgttact ttttgtactt
    26221 tccagctcga tttttatttt cataggcatt tgacttattg taaaatcctc gtcgtttatt
    26281 tctttttggc agttgcttcc gttcccaacc aggaatactg ctaataatca aggcaatctt
    26341 tttagtttct gcatctgatc gaccattcaa taaatccttt gcttgtttat caaaaacagc
    26401 ttccaaaata tcagcggtga gtacgctatc aactaaataa aactcttgtt gcatagtagc
    26461 tccattatag aaatgctgta aatttttctc attacattta ctgtcaggat agttctgata
    26521 ataagaacgt ctcgcccacg ctggagcttc ttcccattta tacgggattt ccatttccaa
    26581 atatttagtg attgcttctt taattggatt ctccatcttg gcatcctctt gatagtcttt
    26641 tgcaatttct aagacttctt tatcttcatc gggactaaaa tagattcgtt gtcctttatc
    26701 aaaaagcacc tttgcttcag ctaacacttg aagaaaatat tcatcctttg ttttaaacgg
    26761 attcatcttt ggcttattct tacaaggcaa aggataaaaa cggcggtttc ctgtatcatc
    26821 tttcaaatac tgcccatcat tcgacgttcc aataaataca cattgccttg cccaagctat
    26881 gacattccga ccataaggag gtctaatttt atcgatcttg gcactgatga agttttttat
    26941 ctgttcaatt ttagttttgt tcattgaaga taattctcca agttcaataa ttacattgcc
    27001 ttgaagctgc atataatcat ctttcttttc acctagcgaa tctaatgaat cggtgaaaaa
    27061 atcatccgtg taaagtaatg ctggaacggt actcttacca atcccttgcg gtccatccaa
    27121 aactggaacg atctcaaact ttattcctgg tttatagata cgtgcgactg atccgactat
    27181 ccaacgtttt gttacttctc gtgtgtaaag gttgtcgtct acccctaaaa ggtcaataaa
    27241 cagtgtttcc agtctaggct gttgatccca tttaactttt tcgatacgtt cttttactgg
    27301 gttgaaaaca ttctttctag ccacatttac tactgctttg tgtattttgt tgtcatcaaa
    27361 aactacattg tagttttctt caatatatgc cgttaaggca tcctcaaaac tgtcttccca
    27421 ttctccagcg gtacgattga tccaaggaga atcttttaat aggtgaatgt atccgctaaa
    27481 ttcgttataa gcaattgttc cagctaaatt gctatcattg gttaaaatca attctgcatt
    27541 cacaacactg ttacttttaa tctgatcgtc tttattgcat cttaatcgtg acatccaggt
    27601 tttcaattca atgatattgt cttcacgctc gctggcttcg ggaaagtcta aaatactatc
    27661 taatttatgc ttatgagcca tgagctttcc ccctcaaatc ttttttaatc atcgatcgaa
    27721 taatttgatt cacttcttta tcttttagcg gtggttcatt gtacgcattt gctgtataaa
    27781 tcaattctgc taaagtttga ttatctacac cgtgccatag taaccaaccg cataacttag
    27841 ttaaatgatc gtttctacta ccttcgccag cgccttgaac aatcatatct aatttttttg
    27901 ctgtccctgt ttttgctacg ggtcggtatt gagtctttat ttcttgcggt ttttttagca
    27961 gtaggttcac tagccaatcg ggagctggtg caatattatt tagagatacg ttttcaatag
    28021 gttcatatgc tctcccaaca cttcttgaaa taatcatagt tggcgctatt ggaactcctt
    28081 tagtgataat atcaatcccg cttaaacttt tacctttatc atcacgcccg aagatatcag
    28141 caaatgcacc tgtttcttgt tggataatta caccatcagg aattttaaag aagaaatgga
    28201 ttcctttacc tggtgtgatt tccgtataag tagaaggaaa tggatcatac attgaagcaa
    28261 gttttttata attctcaacg ccgtttcgtg tttcagaatg ttgatctagg tcaactacca
    28321 ccatttgatg atcttcaagc attaacccaa tatttctagt tgcatcttcc gtccaccatt
    28381 cattaatttg attagaatcg ttagttgcat cattccaacc actagagcct tttattggaa
    28441 ttttagttcc aggagataag ggataaattg accatcccga cttttgtaat tccgttgctg
    28501 cttgataatt atttgccaat aaacctctcc tcctttttca aaatactggt ataaatcatt
    28561 cagacacctc catgagcttc aactgctcaa gttcgtattt caacccatcc cgaacaaatt
    28621 cagcaccttt taattgattc tgatacttta cccatgtatt aaatgttttc acccttgctt
    28681 cttgcatatc agtaatgttt aaccatgcgg ttaaaatctg attttttagc tcgttgattt
    28741 ctcgacaaat tattttgtgt tctgcatgtt ttttgagtac atacaaaact gttagttttt
    28801 cattgaatga atttccaaag taggttttcc cgatattatt ttctagttca gcgatccctt
    28861 gattaatttc ctctaattca aggctatcga gttggtttaa tagttttgtt ttgtacatct
    28921 acttatgccc tccagttctt aattgatagt cgtactcgtc ataagcagtg aagagatata
    28981 acgctaatcc tgcaaagata gctatttttg caattaatgg taataagccg aaaattccgc
    29041 cgattaaaat gcttcctata attaagtttc gtttcataat ggttccctcc gattttttaa
    29101 tatggtataa tcggaacaaa gaatatcggg ttttatccca attaagttgc ctttatcctg
    29161 tccgccaaga ctgttatgga taaggctttt tctttgttct cgatcatgtt aatgctccta
    29221 agttgtctgc aattcagcag aacgattata aattttctca ctcactgaaa actgcaaccc
    29281 atatttttct ttaacagtca ttaattctac tgtctcatct aaaataggtt cacgatcaac
    29341 caacatttca ggagtcatat ctttcttttt taccattttg gaatgaccat aaatagttga
    29401 tacagcttta ttggctatca cattcgcttt aatgtagtct actggttttg gttcagtaat
    29461 agcgtggctt aatttagtca ttgcttcttt ttggtgttct ttgtctagca tgcggaatac
    29521 ttgaaagcct tcaagtcctg tggactggcg tagttctttg atgatgtcga aaatccagtc
    29581 ttgaaatgcc tcggcttctt ttttacggct tttgaatgcc atgcggtaaa tatttttttt
    29641 cgttgatgat gtttacatct tgtgttctgc ccaatgaatc gatgacctta ctagtagtaa
    29701 ccccatcttt aggcaaagag tgaatggctc tagtaacctg tttaagacct aacgcttccg
    29761 caacatcttt cgccactgcc caccattcat cattaatatc gacaaatctt ataatgtgtc
    29821 cgttccaaat ttcagttttc attcaacgtc ctccagttca agccctagca agctcgctaa
    29881 caactcaact tgctgataca aaatttcttg atactcttca atagaaagtc gttctttaaa
    29941 ctctgcttgt actaaatcgt tcaagccacg caatactccg cttaccgttg gttcttgtgg
    30001 tagcaatttt tgatagtcca tttgttcgta caaacattca aacgtttctt ctagcgcttt
    30061 gccttccaag tttgctaatt ctgccgctgt gtcttctgta agaattgttg ataatacttt
    30121 ttcgattgct ttattttgtg tcgattccat tattatttcc tcctattaat ccctttgccc
    30181 ccacggcttg cgtttatttt tggtattctt ccataaagcg atcacattca tcttgtgaaa
    30241 ttcttacgac tccatcaact ataatcaact ttaaaccgtt aggaacatat ttctgcatca
    30301 tagtattgta tgaaacgttc atatacttag cagcttgtgc cttgttcata tattttggaa
    30361 actggtactt tttttctaat aatgaaagta acgttccttg agtttgctct acttgttgaa
    30421 caactcgatc gtcaataatt ttaaaagctg gatcatcaag cgtgagtttg tcaattttta
    30481 attccatatt tatcccccct cgttagcata catttctata caaagatttt aaaaaaatgc
    30541 aagaggctct tgaatacatt tctatacctc ttataaactc aatataccat catacctttt
    30601 catttgcaag aattttaaca tacttttttg aacttttatg tttcacacat atttaaatcg
    30661 tgatatagta gagaaaatat atgaatggtg gtgaaaatgt ggctagaaaa aaataccccg
    30721 aagcagtgga gacaggacaa agaattagac tggctagaat tgaaaaggga ctctcaatgg
    30781 aacaacttgg aggtaaatta tccccaccgg ccagtaaagg tgccgttagt aattgggaaa
    30841 atggctataa tcttcccaat aacaccaggc ttaaacaact ttcaaaggta ttagatgtat
    30901 ccactaccta tctattaaac ggatcttata cattaagaga tatgcatatg atgcccgaaa
    30961 ttaagcgaaa tgtgcttgaa aagcaattga aaaaaactat tgaaaaaaat aactctataa
    31021 ttgattctaa tctttctaat gctatcaata acatcgaaga agataacctg actgtctcac
    31081 agaagattta tttaacaaac gcaataaaat atcttaaaac aggatctcga attgaatttc
    31141 ctgacgggag taccccaatt gcttatctcg cagctcttct aaatacgctt ttacgatatg
    31201 actcattaat taatgattca gaaaaaaata cagatgagaa aaaagagtat caagaatttt
    31261 atgaaaatga tcttctaaaa gagttcacag ctcttgttac tgagctaaat aaacactatt
    31321 caaaataata ataaactgcc tcctccaacc gcccccacgg cttgctacac tatggaggga
    31381 aaactattat ggcaacattc aagcaatatg aaactaaaaa aggtaaattt tggctctatg
    31441 aagcctatct tggaatgaac aaaatgactg gcaaacctga taaggttcgt agacgaggtt
    31501 acaaaacaaa aaaagaagcc cagcttgcct tgtcacgctt gcaagtggac tacgataaaa
    31561 acggtattaa aaaagcgaac aacgaaacgt ttaaagaagt ttatgacttg tggctagaaa
    31621 catataaaac cactgtccga gaagccacct tcatgaaaac tgaaatcaag tttagaaagt
    31681 ggattcttcc aaaatatgga aatcttcgtg tgaatgaagt cacggtaaaa aaagcccaac
    31741 aaattgtaaa tagctgggct gaaactactg accaatacaa ggtacttcat tcaacttcta
    31801 agcgtatttt tagctacgct atcaatcttg ggattattga gactaatcca ctagacaacg
    31861 tattaatgcc taaaagaacc accactaagc ataaagaaaa ggtcaaagtt tacagtaaag
    31921 aacaactgat caccttgttt gattacctaa acagcaaacc tgaaacttat cgaaacgact
    31981 ttgataaaac gttactacgc tttctctttt atagcggggt taggatcagt gaagcccttg
    32041 cattgaattg gtcagacatt gattttcaag aaaatacggt caccatcaat aaaacactaa
    32101 gccaatccaa aaatggctat aaaatcagtg atcctaaaac tgattgtagt gctggggttc
    32161 ttccactaga tgaaagaaca atttctatat taaaaaaatg gcagattaat cagcggaaat
    32221 atatgctgac acttggtgta actgatccga ctatgatttt ttgtggtatc tataaagaac
    32281 taatcacaca ccacgctatt tatgcaagat tgattactat ttgtgaaaaa gctatggttc
    32341 catttctcgg tgtgcatgta gcaagacaca ctcatgcttc aatgctcttg gacgctggag
    32401 ctactatggt agaagttcaa actagattac ggcatacaaa aattagtcag acgatcgatt
    32461 gttacggtca cttggcaaaa gaaacgaaag aaaagactgt tgaaaaatta gtacaacact
    32521 taaatattga ctagatggca tacaatatgg catacagtaa aaaataaaaa aaggagaaac
    32581 cctatcatat caaggtttct cctagcttta tagtctattc ttcttcttcc gccatgaatg
    32641 tattgaatac ttcttcaatc atgtcccatt ctgcgtctgt ttcgatttgt tctaactcgc
    32701 cttctgttcc atcttcgttt tcgatgtatg cataagcttg taattctaca tcttcatctt
    32761 ctgaaacacc agcaggatat aaaagaacat agttacgtcc aaattcttct tgtccgtcaa
    32821 ctgttagtag gatttcatat aatgtttcat ttccttggtc atccaccaat gtgatgtgtt
    32881 cgtgtccttc atggtcgtgg gaatggttat gttctgtcat aagattacct cttttctttt
    32941 attattaaaa taattgctta cctgagccgt ctaaataatt ttgtaagatc atcacagctg
    33001 ctaatttatc gatgactttt tttcgtttgg ctcttgaagt atttgcctgc tctactagca
    33061 tacgttctgc ctgaacagtg gttaatcgtt catcttgata atcaactggc aaggagaata
    33121 gttctttgat cttttctccg tatgacatgg aagcttctgc ccttggtccg atggaattgt
    33181 tcatattttt aggtaatcca actacaaacc ggtctacttc atattgtgct actaattcct
    33241 tcaaccgatc aaagccaaac tcttcttctg cttcattgat ccgaatgatc tctactcctt
    33301 gagctgtcca gccaaagggg tcactgatag caacacctac tgtcttagaa ccgacatcca
    33361 agcccattac tctcataaat tcactccatg atttgagagg tagtccttga caattacttc
    33421 catgatctca tcgcgttcgt gacggcgaat caaatttcga gcatcacgat aacgaggaat
    33481 gtaggacggg tctccagaaa gcaagtagcc gacaatctga ttgattggat tgtagccttt
    33541 ttcttccaaa gcccgataaa caatcgctaa tgtttcgctc acttcttttt tgcgactatc
    33601 gtcaaaacca aaacgtacgg tttcatctgt aaaacccatg attctacacc tctttcccgc
    33661 aaggtaattc cagcttacct tctacaacct tattttactc gaagaaaggg aaaactacaa
    33721 acaaatattc caaacttccg aataaattaa gaaacactat ttgtatgttt ttttcctttt
    33781 ttctgtagct ttatcaaaag tactcttttc ataaagtata cgctatattt ctttgaatgt
    33841 atgtttttac acaatttttt cataatattc ataaaaaatt cgaatagctg ctactaattt
    33901 tttgtttttg ttgcttgtcc tgtctcataa ttgatcgtgt agccatcttg aacattgtag
    33961 ataaagacat tgaattcaat ttttttgtct tcaatgcttt ctgcttccaa ttgcactccc
    34021 ctggctacca gttcattccc ctcaaaatga ggagtgacac ggtagcgtac atgatgattt
    34081 gtttctttga tatatgccgc aatatcattt tcgtgagcaa gcatgtatgg agcattcaac
    34141 gaacgtgtcc ctgtcatcaa gtttttaatg ttgttatttt gaccagtcag ctggaaacca
    34201 attaaatggc tgcgattata taaccattgc ccgtcactta atttcttttg tttccaacct
    34261 gtcgggtcaa tatacaaagg ctctctcttt tcagtaggga acaaatcttt tccaagcata
    34321 gcattggctt gtcctactct gttcaattta tcaatatttg aatagctttc ccatgttcct
    34381 ttggaaaggc taagatcctc ttttgtaaat tccggctgat tcccattgat ttctatttct
    34441 tgatgttttc catcatattc tggcacatcc gctacttgag aagcttgtgg tgtggaatca
    34501 aaaatagttg taatctgctg aaagatatct gaatcttttt gtgtggtgga aacaccgaat
    34561 gcccctgcaa tcacagcagc gacgatcccc acgatcgcct tggttgtttt tttgctcatt
    34621 tctttactcc tttactaaca gctccctaaa tcttaacata tgttccccta taaataaata
    34681 ctagaaagaa atagaaacag caaaaaataa aacctgtttt aagatggctt aaaaatacaa
    34741 aaaagagacg gattcctcgt ctctaatcag tcaccactct atctgtaagg tatttattat
    34801 tattcaaaaa ttttcttcat actcaagaca atacactgat ttcacaaccg cttggtaaac
    34861 ggtgcgcaaa agcttgcatc tgcggtaata agtccatcca agttaaaaac ataagaagtt
    34921 gtttttgctt ggctgttctt attacttgtt gcagagcagc tttttcacaa cctattttaa
    34981 gcgggtgacg ggaatcgaac ccgcgacatc agcttgggaa gctgggattt taccactaaa
    35041 ctacacccgc aatacaaaaa cagtatacgc ctcttttatt ccttcctcaa gtttttttat
    35101 tctttacgct tctggtatgt tttgtagtaa aataaaacta atcaatcaat tattggagaa
    35161 gatgctatga cgggaaaaga attagatctg ttgctggaca cttgtttact cgctgggaaa
    35221 atcatgatgg aaagcaatgc ggagatgtat cgggtagaag atacgatgag tcgaattgct
    35281 ttagcttctg gaaatttccg tttggtcagc tatgtcactc aaaccggtct ctttattggt
    35341 cttgatcgta cctcgaccat ccgaatggaa caaattacca accgttcgat taatttagaa
    35401 aaagtagtaa aagtaaatga tctttcacgt aaatatgttg cttcagagct aacattagaa
    35461 gaactttact gcgaactgca agcaatcgac cgagaccgtt ctttctttcc tgtctggttg
    35521 caaatcgtta gcgcggcagt gatcagtgga acaattatgg tgttatttgg cggaaagatg
    35581 accgatttac ctgtgacttt agtcattgga ggattgggat acgcagctta tctatatagt
    35641 ttgaaatttt ttagaatcaa atttttatcg gaatttttat cttctttatt gattggcata
    35701 gccgctatct acagcgtacg ttttggctta ggaagcaatc aggatctaat cattatcgga
    35761 tgtgtgatgc ctttagttcc tggagttcaa attacgaacg ctatccgtga tttgctagct
    35821 ggacattatg tctcaggagt ttctcgcgga acagaagcga tgatgactgc tacgatgatc
    35881 ggttttgcga ttgcttttat tttccagctt ttttattagg aaaggaacac aagtatgggg
    35941 acgatattta ttcagttttc ttttagtttt ttagcgactg cggcatttgc cgttattaca
    36001 aatgtaccta ggcgttcatt gatttgttgt ggtctttctg gtgcttttgg ctggatgatc
    36061 tactggatct gtgttcaaat aggcggatct gctgcttttg gttcattgct tggttcttta
    36121 ggagtagcat tcatcagcga tcttttctct aaacgtttga aaatgcccgt aacgattttc
    36181 aatattcccg ggatggttcc tctagtccct ggtggtttag cttatcaagc ggttcgaaat
    36241 ctcgttactg aatcctatcg agaagcggct cattatgcgg tccaagcaat tatgattgct
    36301 ggagcaatcg ctttaggtct tgtattatca gaagtcttta accataacat ccgtaatttc
    36361 aaagtaaagc gtgacgcaat catgatgaaa cgaaaaaaac ggctttttaa ataaacgaag
    36421 tgtaacaaag aaaaggttgt gaaagagacg ttcagccttg agcgatgaca aaatatctca
    36481 caaacattgc gacaagtaac cgcaggagca ataagaagta attatgagta ttaaaaaaag
    36541 ttttgtaaaa atagcttctc acatttttac ataaaattga cttaatactg agaaactgac
    36601 ttttgaacac cctctatctg taaaaaaggg gctgcgacat gatttttgtc acggcccctc
    36661 ttctttattc aaattttttc caaaacgttc gttgataata aacgatccat agagcggaaa
    36721 gtattagaac taaaccgatc acaggaatat tcactaacca aaatggataa aacaaagcca
    36781 acattccgta taagacgata agaataatac tgatgatcaa tgaacccatc atagttgcga
    36841 ctaaacccaa acttcctgaa cctctgttaa ataattggtt aatattggtc cagttcagca
    36901 gcagcaaccg ataatctcta gcgaaataac gtagtgacaa taagtagcag ccgaataaag
    36961 aaccaaatag caagctaggg ataaaaaaca aaggcagctg aaaagacagt ccgcttaata
    37021 atgcgatccc accagttaaa aaactctgca atatccatcc gaatctgaac ttttctttta
    37081 aatattggtt catagagatt ggcaaagaac ggataaacag aaaattttct tgatccaaag
    37141 aaataagatt gctgataaaa gaagtctgat taactgttaa gaaagccaat gcgatcccta
    37201 ccaggaaaac aacaccgatt aacttattat ctaaatcagt taattttatt tcgcctccta
    37261 gggcaaaagc aagaataaaa atgattggag ttaacaagga agaagaaaga acttgcataa
    37321 tcaaattagg ttcttttact aattgagcat tgtaactaaa taaaagctga tgcacatttt
    37381 ggttcttttt atgggttctt tttattttga actcactgtt tgaatcagtc agttgctctg
    37441 taagctttgg tactatcagc tgttttacag caaggaaaaa tcccagagcc gtgatagcta
    37501 gtattcccca acccaataaa ccttgttgag aaagtggctg actggcagca tcatagaatg
    37561 gcaataatat cgaaatcgga tgccgatctg ttaactcatt ttcaatagtc cccgtttgat
    37621 gattcatcca catgattcca gcaaatgcga tcagcatcga gctgccaagc aagatactag
    37681 aaactgtttt tctatgtttt ttaaacactg ctgttcgagc caaaccaaaa acaatcaaac
    37741 tagccagaca aaaaagaata atcaaaaata gagaaaacaa aaggattgag ataatgatag
    37801 taagcaaaat ccctagatca gctcgccatc cggttaaaat aaacatgact agaagcggaa
    37861 aaacaaaagg aatgatggtc aaagcaacta ccaaaatctt tgctgaaaaa acctgttctt
    37921 gtccaaaagg cagaggcaaa aatacttgca ggtcatttcc ttcaaaaaag atattataaa
    37981 tgactgatat actttgagtt aaacctaaca taccaaaaag tgccacgtaa taagtaaaga
    38041 aacctggcaa tttaacaaag ttcatagcaa acatagcaaa tccgtaaatc agtaaaaaaa
    38101 tcagccctga aaaaagattt tgagacaaaa gatagatagt taatcgcttt cccttttttc
    38161 ctttctctct tgctttattc gtgagctgtg gattaacata taaaagatta actcgaatta
    38221 attctaataa ttgtcgctta tacatacggt ccatctcctg tcgcttgcct tccagccatt
    38281 ttcaaataaa tcttttctag cgtttcatca ggtgagccat tcaataattc actaactgcc
    38341 ccctgataaa tcaattcacc tttcttcaaa atagccagtt tatcgcataa ctgttgtgcg
    38401 gtatccaata catgtgtaga aaaaatgact gtttttcctt ttgcggcatg agccttcatc
    38461 atttctttca aatcaaatgc tgcttgagga tcaagtcctt gcaaaggttc atccaatacc
    38521 caaatatctg gatctggcag caacgtgccg atcaaaatcg ttttttgacg cattccatgt
    38581 gaaaaacttg cgattgtttc attttgatga ctatacatat caaaaagcgc tgtcaattct
    38641 gctaaacgct tttctttttt ttgtgtgtct aattcatagg cagctgaaat caagtcccaa
    38701 tattctccag ccgtcaattg taaaaaaata tctggggaat caggcacata accgattttt
    38761 tgtttgatac tcaatcgatt ttccgtcaat ttcattccat ctactaaaat cgttcccgat
    38821 gatggttcaa taatactaac taaactttta atcgtagttg attttccggc accattatgt
    38881 cctaaaaatc caaaaatttc cccttgtctt atcgagagag acaagtcttt taatgcttgt
    38941 tttttcccgt aacttttact tacattgatg aattcaatca tttctgtact ccttttgttt
    39001 gttattactt tttattgtac ccttcgttat tggtggagta aatgtcaaaa agagtgcata
    39061 acaaaaaagg atagaaacga ctaggctgct acttgggttg ttctcttcta caggaaaaca
    39121 ctcttctaac aggtaagata tgagtctttg ttttctttaa aatgattcat tttctatctt
    39181 ttcttgcata aaattcttta gtttatcaat acttctcttt cccatctcta gttgttctcc
    39241 atttgcaaat gttactattt cgtttactct gctaatagat gtaatgtttt cagtattaat
    39301 gatataagct tttaaatctt tacataaata ctcatattcg agtgattttt tatatttttt
    39361 aattgtgccg tttacgagaa tctcttcatc tttcaatact aggcctattt tatttttata
    39421 acctggcatg ctcattagat aaaggatttc atcgtaatgg atatagtgat cgctattacc
    39481 ataacggaca gttgtccacc tttttttcgt agcatgttga acaggatagt tttctttttc
    39541 tttcaataag taactttgga tgcttttttt atttttttca taagtgaatt cttttatcaa
    39601 ataaccacta ggcgtaatct ttcgattaat aacagttatt gctttattcg gatgagacgt
    39661 tagataaaca attttacact tttcattctt ttctctaagc atttcgccta gttcgatacc
    39721 gctataatag gcgttcaaat cgatgtctag gaaaaaaata tctgaggaat agatttcatt
    39781 atttttaatg aattcaacaa attgtataac gttcaatccc tgaacgatct caaatttcca
    39841 aggacttaat aaaattctgg attcttccaa aatttctact agcatttttt caaaaaccaa
    39901 actatcatcc aaaataaata tacgcgttac cataaaaagt acctccactc gatattttac
    39961 tatcaaaatc atttcataca acttttcaac atacatgtta ggaataaatt gtaattctct
    40021 tcacaatctt ttccaacacc tttgaaaccg atatcaaata ctttcaggat gctgcttcta
    40081 aaataaatat caaacaaatg aatacatcat gcaaaaaaag ccagacaacc gacgtccttg
    40141 cgtgtcagct gtctgagcta tgtttattta ttttgttctt ttaaaaagaa cgatacacat
    40201 actgattttt cggagcagag actttctcag cgtttgtaac aactacagat gggatttcac
    40261 gcttgaatgg ttgataatag tctgattgga tggttccttc tattttatac cattcatcat
    40321 ttttaaccgt tactccttcc ggcatatgcg tcaacaaacc aaagactcct gaatctgcca
    40381 cacaatggat aatgccgaat cggaacaaga agatatctgt attgctatcg ttcggtgttt
    40441 ggtaaacaaa tccttcatag ctgatttttt tgccaatgaa ttcgctaggg taattataaa
    40501 ttagctccat aacttctaaa tagttttcat ctgtgatctt gatagtttgc ttatccccat
    40561 acttttcttt caactcattc atttggtcta agtagtctga tttgttgaaa taaatactgg
    40621 tatccggctt taagtattgt gtctgcatct ccggatcccc tactgattct ttacttactg
    40681 ggaaattaaa tcctttggct tctacgatcg tagtgtcgag actaactgtt ggaaacaacg
    40741 tccccacgat tactggcaaa gctagcagta cataagcgat tccacgttga taaggttttg
    40801 acaacccatg gtcatgatcg tgctcgtgtg ccttttcttc tgcttttccg tttttgaccc
    40861 acaaatacag ctgaactaaa gccaaaagag cagataaaat cattgaaaga atagctaagt
    40921 agcgataatg gacattgata tattgatcga gtttgccaga aacttgcaag tacatcatca
    40981 attcgaaata cccagataaa atcaaaaacc gcagcatatt ttccctccta aataaataat
    41041 gtgaatccaa taacgctaag ggcaatcagt ccgatcagcg ataaaataaa gcgtcctgta
    41101 aaatatcgtt tcatcatcag tagatttttg atatcgacca ttgggccaaa gaccaaaaag
    41161 gcagctacag gtgctgtgcc gaacagactt aacaaagaag atcctataaa cgcatctgct
    41221 tctgagcaaa gagacatcgt aaaggccaat aacagcatga tgataatggc taaaatcttt
    41281 gttgagccga gcgttagcat aattctagtt ggtaaataag tctgcatcgc agctgcgatt
    41341 aatccgccaa tgatcaaata acgacctgta tcaaaaaatt cgtcgattga atgtgtcaac
    41401 acatgcccag tttgatggat cagctgattt tttggttcat tcttctcact ttgttcttct
    41461 tcatggacat gatgatggtg attttctgct actttagacg tgattttttc tttcaataca
    41521 gcgtcttttt gaaaataggc aagccagaca ccaatcacaa gggcaacaaa aaagctgcct
    41581 atcacccgcc agcttgccat ctgccaagaa ttgccaaatg cgataaaagt ggaaaaaatc
    41641 acgattggat tgatgatcgg agcagtcagc ataaaagcaa acgctgtgtg aactggtacg
    41701 ccttttttaa caaattgatg aacgatcggt acgatgccgc attcacatga aggaaagaaa
    41761 aatcctaaga tcgaaccagt aaaaatagag aagatacgat tttcaggaag ccatctcttc
    41821 acacgttctg gggtcaaaaa tacttgtaag gcgcctgaga taagacagcc taataatacg
    41881 aaaggtaatg cttcaataac gatcgataag aaaatcgtac tcatctgcaa aaccgaattt
    41941 ggaataaaag aaaacatgag tttcctcctt tttatttcta gttcattcgc tttgcaaata
    42001 tacgctttta ttcaaaaaaa acaagttgct ctgtaaattt ttacacaact cttatttttt
    42061 accaaagcac ttcaattgac tttttctgtt attcccatta aattaaggtc tcatacatat
    42121 cacttctatc ataaaaggga gaataaaaat gaaacaaacc actaacttga ctgaagttca
    42181 agacatactg caacagtcat ccgttgccgt catttatctt tcgatgccta attgcagtgt
    42241 ttgtcacgct gttcgtccac gactagagga acttctcaca cattatgata ttccggcact
    42301 tcatttggat gcatttgaaa caccagaagt agctagtaaa tttgaagtgc tgactgcgcc
    42361 agttgtcctg atttttcatc aaggaaaaga agtctttcgg caagcacggt tcatcgactt
    42421 taaaaaaatc cgttatctac tagatgaact gacagcagaa gatgattccc tttcttatga
    42481 agaaatcttc cgaacaaaat aatcgttgtc caaacagaaa aaagcgattg aacaagtgaa
    42541 agttttgcct tcatttgttc aatcgctttt tatttttatt ctgctgtatt ttcggaatgc
    42601 gcatttgcgt cagaatgcaa gaaatagatc aaggtttgca attcattcgt caaatcaaca
    42661 ttttggatca atacatctga cggagcattc aatcgagcag cggtaaagtt caagattcct
    42721 ttgattcctg cttccgttaa ctgattgact acttcttgtg ctctttcagc gggtatcgtt
    42781 aaaattgcta cttcgatttg ttggatcttg atttgttctg acatatcttc cattggataa
    42841 acggggatac cgtctacgat gcgcccaacg atttcctcat tcacatcaaa agcacaactg
    42901 atacgtatgc tattactttg atggaatttg tattttagta aagcacttcc caagttccct
    42961 acaccgatca gcgcaacatt tgtcaattga tcgtcgttta atgtctttgc aaaaaagtgc
    43021 ataagatctt ctacatcata accataccca cgttttccta attcgccaaa ataagaaaaa
    43081 tctcgacgga tcgttgcact atctacttga acggcttcac ttaattcagt tgaagacact
    43141 tttttctttc cagcattatt taaaatcctt aaatagcgat aatacaaagg cagtctttta
    43201 gctgtcgcct ttggaataat atgatctttc acgaaatgac ctccacgtat tgtctaatta
    43261 catctttgat ttctccatca tagcagtatt tataggaaaa aatcaattcc attgctcaga
    43321 tattgtgaaa ttttattctt tattcattta tttatcattt ctgcaaatct cgcagtataa
    43381 tggaatttat ggtagactac taattgaaaa atagtttttt atttgaggag aattcacaaa
    43441 gtagaaaaga ggattccatg atattattac aagccaatca gattgctcgc cattttggtg
    43501 cagaagtctt atttgaaaac atacatttag aaatcagtac aggcgctcgt gtcgctttag
    43561 ttggcagaaa tggcgctgga aaatcgactt tattaaagat cattgctgga attgaagcac
    43621 ctgatgaagg aaagatcgct aaaaataaaa cagcgactct cggatacctc gctcaggaca
    43681 caggacttga ttccaatgaa acagtttgga acgagatgct gaaagctttt gaacaagtta
    43741 gacaaatgga agagcggatg cgcgagctag agattttcat tagcgaaggt acgccagaca
    43801 cacctgctta tagttcttat ttaaaagaat acgaccggct gcaacacgat ttttcagatc
    43861 gaaacggcta tggttatgaa aatgaaattc gttccgtatt gcatgggttt aaattcgatt
    43921 cttcatttta tgatcagaaa atcgatactt tatcaggcgg acagcgcaca cgtttagctt
    43981 tagctcgcat gcttttgcaa caccctgaca ttttgattct agatgaacca acgaaccatt
    44041 tagatatcga gacactttcc tggcttgaga attatctgca aagctatact ggtgcattat
    44101 tgattgtttc ccatgaccgt tattttttag ataaagtagt caatgaagtc tatgaattaa
    44161 gcagaagaaa aatgcgccat tacaaaggta actattcgaa atatctagat ttgaaagcag
    44221 aacagttagc aagtgaatgg aaggcttatg aaaagcagca aacagaaatc cataaattag
    44281 aagacttcgt tgctcggaac cttgttcgcg cctctaccac aaaacgagcg caaagccgca
    44341 gaaagacatt agaaaaaatg gaccggctgg atcgtcctca aggggatgaa aaacaagcaa
    44401 actttctatt cagtatagaa aagccttctg gaaatgtagt cttgcaaatc gaagatgccg
    44461 ctatcggata tgataatgtc accttatctg aaccaatcaa tctagatatc cgtcgtcaag
    44521 aagcgattgc tctagtaggt ccgaatggta tcgggaaatc gacgttgctg aaatcaatca
    44581 ttcaccagat cccttttatc cgtggaaaag aaacgctagg gacaaatgtc accatcggct
    44641 attacgatca aggtcaagct gatctgcata caaacaagac gatcctagca gaattgtggg
    44701 atgagcatcc taccactcca gaaaaagaga tccgaagtgc tttaggtgct ttcttatttt
    44761 ctggcgaaga tgtagaaaaa ccaatccctc tgctaagtgg cggtgaaaaa gcacgggttg
    44821 ctttagccaa gttagcgatg aacaaagaaa attttttgat tttggatgaa ccaacaaacc
    44881 atttagatat tgacaacaaa gaagtattag aaaatgcatt gatcgattat gacggcacac
    44941 tgctttttgt ttcccatgac cgatatttca tcaatcggat cgcgacaaaa gtcgtcgaac
    45001 tctctgaaaa tggcagcaaa gtctatctag gagactatga ttattattta gagaaaaagc
    45061 aggaagaaga agaaatcgcc gaattgttgg cacaagatga gccagaatct gctcctgctc
    45121 ccacgaacaa atctgattat taccaaagca aagaacagca aaagcttatc cgtagccttc
    45181 aacgaaaaat tactcaggtt gaagaagaaa tggcgcggat cgatgaatgg atcgatcagt
    45241 tggaacaaga aatggtcgcg ccagaaaacc tggaagatca cgtaaaactc aatgagctga
    45301 accaagattt agaagctgct agacaagatc aagaaacaaa attgacggaa tgggaagaac
    45361 taagcttgga attagaagaa tatgaaaatt aacgaaaaaa ccaaaacaga aaccattcat
    45421 gctgttttgg tttttctttt tttattttat caaacaaaaa ttccatattt acctttttca
    45481 ccaataagaa gaccaattac tagtcattct cactaaagct tctaaataat agtaatcacc
    45541 ccaaatacaa ggtgtattta ccccattatt tttgggctta ttataaacag actgtgttaa
    45601 aaagccatct tcttcttccg ttgtagcata tctttctttc aattgtcgca taattttcca
    45661 aacagcagac tggtataagt tcttttctgt tttgtctaat agacctgatt ctattaattc
    45721 taataatcca caaaccatga tcaccatact agatgtatct ttttcttctc tgctagaatc
    45781 atcaaagatc aaatcccaat aacaaatggt atcttctggc aaccgattca taaaataatt
    45841 ggctaatatt ttgcttaaat caaaatattt ttgttctttc aaataacgat aacttaatgc
    45901 aaaaccgtaa atcccccatg cttggcctct agcccaacag gaattatcac tataaccttg
    45961 atgcgtactt ccatatcttg gttttccatc tttgacatcc atataaaatg tatgataagt
    46021 tgaaccatct tctcttacta aataatctgc tgcattttga atatgagcct gagcagcttt
    46081 tttatatttt tgatttccag tttcttcact cgcccaatac aataatggaa gattcatgca
    46141 acaatccatg atcattctac cttgttgtga aggatcacta ggatctcccc aagcttgtat
    46201 aactcctgcc ttctcccaaa aacgctgaag taatgtttct gcagcatcaa ttgaagcttc
    46261 cttcattttt cgatttcccg ttattttata atctgctacg gttgataaag aatacaaaaa
    46321 accaagatcg tgatgattag tgataatttg acagtcaatc ctgtggataa aatctatatc
    46381 tgctttttca gctgtttgtt gaaataaagg atcacccgtc ttctcaaata acaaccaatt
    46441 aattcctgtc caaaatcctt ctgtccaacc aatattgtct cttaatggat aacgtccttt
    46501 tataggacac tcgcttggga attgatactg actgttactg atacgtttga ttaacctctc
    46561 cgtgtgtctg cgacaacgct cgatctcatt attccattct ttttccatta attctttata
    46621 acattcattt atccattttt ctggatgtat tatctcttca aacatcactt tatcaagcat
    46681 ttccccacca gcctttagca taattttagt ctttttttat tgtttttatt ttggatagtt
    46741 agcccggttg tcccatcaaa atcaattttg ggacgattgt tccatacttc taaactataa
    46801 tcccgatctt gatgagcaac cactcctgtt ccgagccaat gggttccttt tccaaatgta
    46861 ccctctaatc caggaataaa agtgttttcc catgtcatca agtttgtatt gggcaaaccc
    46921 ataatagctg tggattttcg tttaatagta tcgtcactta aacagataat acctgaaaag
    46981 ccagcttcac tcaccctata gttttccttg tcttcttcta tgattattcc ttctttctca
    47041 ggagaacatt caatagcaaa ccctgtctca tatagaacat attctgtatc taattctact
    47101 ttgtgtacgc gaatctgcca gccttcaaaa ggaataagcc aagttctgat ttttgcctgt
    47161 aaagtaggtt cccatgtaga ggcaagtacc tctttcttac atacaatgtc tcgtacatta
    47221 tttcgaacga tgaactgtcc ataggttttg atgctctcat tcttaaaact ggtcgtgata
    47281 tatgtgtccg ctcgctgaat ccctagcatt gaatccattg ccatttctgc tttcaattga
    47341 ttcgctcgcg gtatcgaaaa accaaattga ctagaataag caaattttaa atatttttca
    47401 ttggtcaatg tatggaaatt tggccctaat tgtcccgcat tcaaaaaaaa tgtatgtcca
    47461 ttatcatgtt gtatgagcaa attggcttct gaaagaagtt tagttccttc acgaataggc
    47521 ataggcattt ctttagccgt ccaatactca tggtcttcag gtagcgccaa caataaaaaa
    47581 atcttattca gccataatgg agataacgta ctattatagg gttcagtcaa tgtcaattga
    47641 gggtaggtat aaccaatatt caggatacct ttttcatcaa atatttcttt ttgcatccac
    47701 catcttatat tccgattgac taaccccttg atttcaccta atttaaaagg caaaagatcg
    47761 gcccacaaca tagctgacca aaagcttgaa actgcaaaac gataaatcat gcttcgacca
    47821 aaaggcacat ttgcaccatc ttccgcaaaa aaatgaatat agtctctaga aaaatcaata
    47881 gctcggttct tatatatctg gctaatgggc ttttctggtt ctagtacgct ataaatcaaa
    47941 ccataataat gaaatgcaaa aggattataa taatcttggc gacctcttga actgtctcga
    48001 taccagccat tttgaagata acaattttct atattatcaa atgcttcttt cagtgctgat
    48061 tctttaactg gataaccaaa tttttggata attgttccga caattatctt aaaaaattgc
    48121 caattaccat ctgcacattc atattcaaaa atttgcgaga accaatgaat aatctggctt
    48181 ttttctcctg tatctaattt tttccataat aattgttcat gatgatataa cgctaataac
    48241 attgcgggca tttctaccat acgctggtct ctttcatgta tttctcccca ataatcggga
    48301 tgggaaggat ctgttccagc ttttattcct gatagaatta atttcaacca tcgttcatct
    48361 aattggtcaa ttgcgggccc cgctccccat aaaacccgag agaatccttc cattcctgca
    48421 attttatttc catatcctac cccttgatgt atataatcaa tttgtgcgcc acttgctcga
    48481 taaaacggct ggagtggttg atttatcttt ttaaataagt caaagtaatc ctgtctggtc
    48541 cttaatagtg gtactggatg tttcattgta ttttctcctc tttttactac caaatagcta
    48601 attccgactg ttctaatcga agtaagcctt caataaaaaa ataatcagaa tagatcagtg
    48661 gcacattata agtcagttct ctatggtatg cttctgctcc gcctccaatg atcccatctt
    48721 catccatatt ccaatttgca tagtgctttt ctgtcttttg gagtatattg atagcagctt
    48781 catgatacat ttttgatcgt acagaggaaa catatcctgc taattccaga agaccgcatg
    48841 cagcgcatac tccagcactg gtatcaatat tcgaatcttt tacagcaaaa tcaatgagag
    48901 gaatataacc agttttttcg attttctgaa taaaatttac tgctatcttt tctgctatag
    48961 ctagatagta cggatttttt gtgtaacgat aacttaacgc gaatccatat aaagcccatg
    49021 catttcctct gctccaacaa gaattctcat tgtatccttg cccgccaagt aaccgaatat
    49081 atttccctga ttctgcatca aattctgcga tatgcccggt tgatccatct ttcctcacta
    49141 gatacttagc tgttgtatct gcatgctgcc gagcaatttt agagaacctc ggatcaccag
    49201 tttccctcga agcccaatac agtaatggta aattcatcat actatcaata atcatccaac
    49261 caggtttatg gtcattccat gctactaaat agttccctga aatattatag cgtccagcaa
    49321 gtaccgtagc tgcatgtaac cctagttgtc gactatgttt gttttgagac tgttgataat
    49381 gtgcaatagc cgatggtaag aataaaaaac ctacatcatg atgaacgtct gaaaaatggt
    49441 acagggcaga ttctaataat tgttcttgtt gtattgctgc ctctttgaac ttttctcgtt
    49501 ttgtcttgtg aaagagctgc cataaaatac cactccaaaa accattggtc caccagtcga
    49561 ttcctctgtc ctttattacg tctgtatatt ttccgttgta agaaatatat ggaattttat
    49621 tccccattct gtctaattcc gcatctatct tttcagaagc tacattcaaa atttttcttg
    49681 accattcctt cgtctgttca ttcatggata tcccgccatt cttttaattt ttatgaatgt
    49741 ggagttgctc ctagaacgaa ctagatggtt aacattcgtt atttttttta cttatttgca
    49801 gctccaatgt cattctcctt aaatttagac tgcgcccaca cataaatcaa gaccattggg
    49861 atcactgaag ttgctaaaat tagcataatt gcgccataat taattggctc acctgtacta
    49921 gaaaccgtca tatttttgat aattaaggga accgtatatt tatctttatc tgaaagcatg
    49981 actaatggaa atagatattg attccaactt cccataaaga taagtaaacc actagtaata
    50041 atcgtaggtg ataaaatcgg tacagcgatt ttcgtaaaaa tcacccactc attggcacca
    50101 tcgattctgg cagcctccat cagtgctgta ggaaattgac tgctatattg tcgcatcata
    50161 aacactgaag ttgtactggc aatcgaaggg atgatcacac ctgcataagt attcgataat
    50221 cctaaacgac tcataatgta aaataaagga atcaatgtgg taaaaaaagg caccatccta
    50281 gatagcataa ctgtattgaa caacagttta ttccccataa aacgatactt tgctaatgca
    50341 taacccgcca ttgtagaact ggctgtagct aaaactgtac tgattacagc aataacaatc
    50401 gaattccaaa taatcaaggg ataattatat ctcgccatga tggcttgcca gttttttaat
    50461 agcatatccc caaacttgaa attgattccg ccacttaaaa tttcttgatt actttgcgtt
    50521 gaagctacaa tcatatataa atatggcatc aaaaatatta aggcaccaaa cacaataatg
    50581 acgtacgtaa aaattctgct ttgtatcttt tgtttagtca tttttatcac ctccgaattt
    50641 aaattgaata gccgataata taacagatac aaacaccatc gtccaggcaa tagcagaggc
    50701 ttttccaaaa tcaaccattt caaaacttgt tttatataaa ttgactccta cgaccaaagc
    50761 tgaattttct ggtccattgg aagtcccaaa gaatatattt gggatattga aattacccaa
    50821 gccattgata gtggctaaaa ctgtggccat taaaatgatt ggctttagca tggggatcgt
    50881 taataaaaag aattgccgaa atttcgatgc tccatctatt tcagcacttt cgtatatctc
    50941 tttagaaatc ccttgaagac cagctaaaaa taaaatagtg ttatatccta cactaagcca
    51001 aatcatcgtg atgatcattg atattcgtat ccaaaaagga tttgttaacc actgtacacc
    51061 gttaattcct agcgcattca aaaatccatt gaataaccca gtcggattga aaaaagtcat
    51121 gaatacggaa gctacagcca ccgttgatgt aatggttgga atataataga cagttctaaa
    51181 aaatcctttt ccttttaact gtgctgaatt caataaaaca gcaaagtaaa tagctaatag
    51241 catcgttatt ggaattgatc ctgccatgat aatcaaaaca ttcgtgtaag atttaataaa
    51301 caatggatct cggaagatag ctagataatt gtcaaatcca acaaattcaa atgagtagcc
    51361 aattttcata aaactaaaag caaacgagat aatgatggga atcaaaatcg tcacaataaa
    51421 gaaaataaca aaaggtgtga ccaatccata agcacttttt ttatgagacg tatctgttgc
    51481 ttttttcttg ctgttcttta ttgtcttgct tggtaacgaa gattgcatca tcgttaagtc
    51541 ctcctaatat tttttaatca ccagttaatt ttacgccgta cttatgagca aaattttctg
    51601 cttcttttag atatgaattt tcaaaatcac ctgtttgcca atagttatat gcagcggtag
    51661 tcaaataaga cgtagcatct gaataagtgg ctggtaaaac agtatttgat gtattttcat
    51721 tgattttatt caatagttca tagtattttt gtcctcctta gtagtcaaac tttttctcag
    51781 ctgcttctgt ttgataggcc aatgagttag atgtagccac gcccagctcc agtgcgttgg
    51841 ctaagcattc ttcatctgtc agcataaatg taagaaattg ttgtgccact tgtggatttt
    51901 ttgcatcttt agggacataa aaacttgatc ctcctgatac tgcacctttt cctggatttt
    51961 cttttgaaaa aggaatcagt ggagccatgc gccatttgtc agatgcatca ggaaaattca
    52021 aagacatatt cgtagcatac cagccgccag caacaaacat tgcagattct tgaaatgctt
    52081 tctcagcatc ttgtgcaccg tagaaatgta caatatctga gtcgatcatt tctttgatta
    52141 actttgcagc atgtttcgct tctttagtat tcagacggat atttccttct tcatcaagca
    52201 aagattgatt ctgctgggct agcatgttga taaacaattc ttggtcccca gattctggca
    52261 aggcaattcc aaaactacca gttttttctt ttaatgcttt tgccacctgg atatattctt
    52321 cccaagaact gatttcttca aaatctacac ctatttcagc aaattttttt ggttgataat
    52381 aacaaagtgc tggggtaaaa tcactagccc aaggaataat cgtttcatta ctcgtttgtt
    52441 gacgcaaaat attcaatttt tgatcataaa actgatcgcc atatttgtca taaaatccat
    52501 attcatcagc actataaaaa gcatcttgaa atttttccag cacacctgct gctgaagtgt
    52561 ctgccatgga gataatatcc ggcatatctt cgtgtgttac caattttggg gtgatcattt
    52621 gtactgttgc tgttcccgaa gctacattca caatttttaa gtcaatattt gctccagttt
    52681 tgcggttata gatttttgca gcattttcaa catagtttgt cagtttgccg tttgcgacat
    52741 atacttctaa tgtagtggct ttttttatca ttggatctgc gatgtttgct gttacttctt
    52801 cgttagtttt tccacatgca cttaagctga ataatagggt aatgtttagc aaagctatca
    52861 ccaatttgta tctttttttc atgtctatcc cttctttctt ttcaagttct tatataaaac
    52921 gctttcactt atgtatatta acggaattag taacacaaat atttatataa aaaaata
//
LOCUS       DAZAID010000009        51257 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000009 DAZAID010000000
VERSION     DAZAID010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.009"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..1561)
                     /locus_tag="ACG9BE_000462"
     CDS             complement(56..1561)
                     /locus_tag="ACG9BE_000462"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFN3031243.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1653..2243)
                     /locus_tag="ACG9BE_000463"
     CDS             complement(1653..2243)
                     /locus_tag="ACG9BE_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN3031244.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2422..4068
                     /locus_tag="ACG9BE_000464"
     CDS             2422..4068
                     /locus_tag="ACG9BE_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN3031245.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4117..4836
                     /locus_tag="ACG9BE_000465"
     CDS             4117..4836
                     /locus_tag="ACG9BE_000465"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFN3031246.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5315..6262
                     /locus_tag="ACG9BE_000466"
     CDS             5315..6262
                     /locus_tag="ACG9BE_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3031247.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            6274..7194
                     /locus_tag="ACG9BE_000467"
     CDS             6274..7194
                     /locus_tag="ACG9BE_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3031248.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            7235..8692
                     /locus_tag="ACG9BE_000468"
     CDS             7235..8692
                     /locus_tag="ACG9BE_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3031249.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            9037..9651
                     /locus_tag="ACG9BE_000469"
     CDS             9037..9651
                     /locus_tag="ACG9BE_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN3031250.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            9644..11368
                     /locus_tag="ACG9BE_000470"
     CDS             9644..11368
                     /locus_tag="ACG9BE_000470"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3031251.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            11365..12816
                     /locus_tag="ACG9BE_000471"
     CDS             11365..12816
                     /locus_tag="ACG9BE_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN3031252.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(13289..15442)
                     /locus_tag="ACG9BE_000472"
     CDS             complement(13289..15442)
                     /locus_tag="ACG9BE_000472"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFN3031253.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(15444..16490)
                     /locus_tag="ACG9BE_000473"
     CDS             complement(15444..16490)
                     /locus_tag="ACG9BE_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3031254.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            16640..17929
                     /locus_tag="ACG9BE_000474"
     CDS             16640..17929
                     /locus_tag="ACG9BE_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFN3031255.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            17942..20644
                     /locus_tag="ACG9BE_000475"
     CDS             17942..20644
                     /locus_tag="ACG9BE_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFN3031256.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            20629..21486
                     /locus_tag="ACG9BE_000476"
     CDS             20629..21486
                     /locus_tag="ACG9BE_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN3031257.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(21557..22414)
                     /locus_tag="ACG9BE_000477"
     CDS             complement(21557..22414)
                     /locus_tag="ACG9BE_000477"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN3031258.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            22625..24796
                     /locus_tag="ACG9BE_000478"
     CDS             22625..24796
                     /locus_tag="ACG9BE_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFN3031259.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            24864..25205
                     /locus_tag="ACG9BE_000479"
     CDS             24864..25205
                     /locus_tag="ACG9BE_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN3031260.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(25481..27235)
                     /locus_tag="ACG9BE_000480"
     CDS             complement(25481..27235)
                     /locus_tag="ACG9BE_000480"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3031261.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(27246..28082)
                     /locus_tag="ACG9BE_000481"
     CDS             complement(27246..28082)
                     /locus_tag="ACG9BE_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN3031262.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(28084..28767)
                     /locus_tag="ACG9BE_000482"
     CDS             complement(28084..28767)
                     /locus_tag="ACG9BE_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFN3031263.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(28926..30320)
                     /gene="uxaC"
                     /locus_tag="ACG9BE_000483"
     CDS             complement(28926..30320)
                     /gene="uxaC"
                     /locus_tag="ACG9BE_000483"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFN3031264.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(30359..31858)
                     /locus_tag="ACG9BE_000484"
     CDS             complement(30359..31858)
                     /locus_tag="ACG9BE_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFN3031265.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(31851..33272)
                     /locus_tag="ACG9BE_000485"
     CDS             complement(31851..33272)
                     /locus_tag="ACG9BE_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFN3031266.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(33332..34324)
                     /locus_tag="ACG9BE_000486"
     CDS             complement(33332..34324)
                     /locus_tag="ACG9BE_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3031267.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            34556..36754
                     /locus_tag="ACG9BE_000487"
     CDS             34556..36754
                     /locus_tag="ACG9BE_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3031268.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(37212..38084)
                     /locus_tag="ACG9BE_000488"
     CDS             complement(37212..38084)
                     /locus_tag="ACG9BE_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3031269.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            38384..39358
                     /locus_tag="ACG9BE_000489"
     CDS             38384..39358
                     /locus_tag="ACG9BE_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3031270.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            39380..40072
                     /locus_tag="ACG9BE_000490"
     CDS             39380..40072
                     /locus_tag="ACG9BE_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFN3031271.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            40038..41546
                     /locus_tag="ACG9BE_000491"
     CDS             40038..41546
                     /locus_tag="ACG9BE_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN3031272.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            41620..42723
                     /locus_tag="ACG9BE_000492"
     CDS             41620..42723
                     /locus_tag="ACG9BE_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN3031273.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            42726..44063
                     /locus_tag="ACG9BE_000493"
     CDS             42726..44063
                     /locus_tag="ACG9BE_000493"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFN3031274.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            44050..44928
                     /locus_tag="ACG9BE_000494"
     CDS             44050..44928
                     /locus_tag="ACG9BE_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFN3031275.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            44925..45848
                     /locus_tag="ACG9BE_000495"
     CDS             44925..45848
                     /locus_tag="ACG9BE_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFN3031276.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            45845..46255
                     /locus_tag="ACG9BE_000496"
     CDS             45845..46255
                     /locus_tag="ACG9BE_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFN3031277.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            46259..47827
                     /locus_tag="ACG9BE_000497"
     CDS             46259..47827
                     /locus_tag="ACG9BE_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFN3031278.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            48067..49485
                     /locus_tag="ACG9BE_000498"
     CDS             48067..49485
                     /locus_tag="ACG9BE_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3031279.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            49497..50807
                     /locus_tag="ACG9BE_000499"
     CDS             49497..50807
                     /locus_tag="ACG9BE_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFN3031280.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT        16520 a         9054 c        10465 g        15218 t
ORIGIN      
        1 aaatcacagt cccttttttt cttagaaatg ggaaaccact agtatataaa taccattatt
       61 cttcaatcaa ccgcttcacg attgcataat ccccttcata aggttcatct acaccattga
      121 ttttttgata gggatccacg ccttttcccg caataacgac tgaatcttgc ggagcgcttt
      181 ctgctaaagc gcgtcgaatc gcttcaggac gatccatttc ataatgaacg atcaaatctg
      241 gattagtaat tgcttcattg atttcttccg cgattttttt cggatcttca aatgctgggt
      301 catctgccgt caaaaaggcg atgtcagctg tttctgatag cacttttcca aaatcatgtc
      361 gacgggagat tgctttgttt cctgggctgc ctaatacaac aattacacgg ccatctggat
      421 gttcattttt ggcaaattcc agcaatgttt tcaagcttag ataattatgt gcatagtcca
      481 catagacatg cgctccattg gattggatca attgatccat tcggcctggt acacgagctt
      541 cttttaatcc gtcttgtgca tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg
      601 cactggctgc attttcttgg ttgaatcctc cagccagcaa aatatcataa gcagtatcgc
      661 agatagctaa gcgatcttca tgagaagata aagtaaaacc gtgagtacct ttatctgaac
      721 ggatcacttg ataattgctt tcttccgaac gaccataagt gatcgttgga atctggaata
      781 gatcggctgt ttccctaagt aaatcaaaat agtcactttc atgatttaaa acaactgttt
      841 ttgaattcaa gatcaattgt cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga
      901 tcggactgat atggtcaggc gagatattca agaagattcc gacatctaaa gtcaagccgt
      961 atacgcgttg tgttttgtaa gcttgtgaag aaacttccat cactagatga gtcataccat
     1021 tttcaactgc ttctgacatc atacgatata aatctaatga ctcaggcgtt gtcaaatggg
     1081 atttgaaata tgttttgcca tctaacgttg tattcatagt agaaagcaat gctgtttttt
     1141 tatttgttgt gtgatccaag attgcttttg taaagtacgc agcagttgtt ttaccttttg
     1201 ttccagtgaa tccgatcact ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa
     1261 gaagcgccat tgctttacgg atatccgtta cgatgattcc tactgctgaa ttttcgtatg
     1321 gttgttctga tacataatat tgaataccct gttcgattgc tgaatccaaa tattcttctt
     1381 tgaaattcat tcctttacag aaaaacaaag tagaagcatc agcttttctt gaatcataac
     1441 ttaactgaag aaattcagct ttttctggta catcaagatg ccagcctctt gttgaaacaa
     1501 attcttttaa tagattttct tttaatagta aatgacggat gtcttctaaa gagaagctca
     1561 ttgattgatc cctcgcttta ttcaatttct tggtttatta tagcacactc tttttaatat
     1621 atcgtttgtt tccgctatga caaaatccag ctttattcaa attttcttga cacaggggac
     1681 aagcggatga gtattttcat aaacaatgaa ctgactgtaa tagataggag aacgaatagt
     1741 gtctccaaca atccaaaaac ataataattc aaaaagacga taagactatt ggtaagtaaa
     1801 agtccttgcg gcattggaag ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata
     1861 ccgccatttg aggccccgac tgtgtacaat gtgtaaaccc caagactgaa caaaagactt
     1921 cctgaaatca gtgaaaagaa atgtgaggag atcatttcca cttttggaac aatctctaaa
     1981 acaacaggaa aaagcagact tcccgctaca gtcttaaaaa atactgattt tcctaatagg
     2041 acagcggcta gaagcagcat cataagattg atgctccaaa cagtaactga gcgttggata
     2101 gaaaaggccg tttcaactaa atgtccgatg gcgcccactc cggcggaagc tatatcgtga
     2161 gggagcaaga accaattcaa tccaactgtg ataaagaaaa gacctaaaaa tataacgggt
     2221 agttttttta tttcttgtgt cattgatatc tcctataaat gttagccata aaaggctgta
     2281 tatttcttta ctttaccaaa aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa
     2341 agttatataa aatcgattgc cagtttctgt tttaccaatt aaaatgatag taatgttttg
     2401 tagaaagttg gaatctgacc gatggataat caaagaaaac cagcattaga tcaattaaca
     2461 ccgcaagaaa aaatggcacg aggctctgct tggatgaccg ccagtaatat catttcaaga
     2521 ttgctaggcg ctgtctatat cattccctgg tatgcttgga tgggcgaaaa tgctaaggct
     2581 gccaatggat tattcaatat gggatacaat atatatgcat tgtttctact gatctctaca
     2641 gcaggaatac ctgctgcgat tgcaaaacaa actgcacgct ataattcatt gaatgaatat
     2701 ggcacttcac gacgattatt tatccgtgca ttacagatga tgggcgggct aggcctgttg
     2761 tttgctttgt tcatgtacat cgcctctcct tggctagctc atgcttctgg tggcggagaa
     2821 gaactgattc caacgatgcg ttctttgagt atcgctgtat tggtttttcc ttgtatgagt
     2881 gtgatacgag ggtattttca aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt
     2941 gtagaacagg tggcacgtat tttttatatg ctattggcta cattcatcat catgaaagtc
     3001 atggatggtg actatgtgac ggcggtcacg caatcaactt tcgcggcatt tatcggtatg
     3061 ctagcaagta ttcttgtcct tttgtatttt ttgaagaaac atcaagcgta tacatcggcg
     3121 tttattcact acagcgaaaa caaaataaat attgcaacga aggaattgtt gttagatacc
     3181 gttaaagaag cgattccgtt tattattgtg ggttcagggg taacgatctt caaattagtc
     3241 gatcagttca cttttatgaa gatcatgtct gcatccaccg agtattccaa tgcacagtta
     3301 ctggatcttt tttcgatttt tagtgccaat ccagataagt tgacgatggt agttattgca
     3361 ttggcaacat cgatcgcgtc tactgggtta ccgttgatta cagaagctgt tacgttgaaa
     3421 gatcgagtag gtctggcaaa attgacaagc ggaaatttac agttgttttc attttttatg
     3481 tttccggcga catttggtgt tatgttactt gcctatccgc taaatacatt attttataca
     3541 ccggattctc taggaagtaa tgtactgatc caagcaagtt ttgtaggact cttcctagga
     3601 ctatatatgc tagtttccaa catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat
     3661 tatttggtta ttgggtttct agtgaaactc cttcttcaat acccagccat tcgtttattt
     3721 gaagtatacg gccctttatt ggcgacaatg atcggatttg ctgtgtcttg taccttgatc
     3781 ttaaagagaa tccatgaagt agcccgcttt aaaccaagtt ttgtctggcg caggacattg
     3841 ctgatcttta ttcttacctt gattatgctt ttggcttcgt ggatcacaaa gatgattttt
     3901 ggtgcttttt taaacgaaga tagcaaattc cagtctctat tgttgattct tgtagtagca
     3961 ggtatagggg ctttagtgta tggttattta gctttgaaaa tccgcttggc agaaagacta
     4021 ctaggaccag gaatagcgcg tttacgacat aaattaaaaa taaaataagt gaatcagaag
     4081 agctagattg atgctctttt gaaatggggg aaacgcatgc gtttagataa gtttttagca
     4141 gatatgggac ttggcagccg caaagaagtc aaaggattga tcaaaaaagg aacgatcgaa
     4201 gtcaatggac aagtggtgaa aagcgataaa gcgcaagtaa atgaattcga agatgcggtt
     4261 acttatttag gagaaatcat tacgtatcaa aaagattttt actacatttt gcataaacct
     4321 gcaggagtca tttctgctac tcaggataat catgatcaaa ccgtaatcga tttattggaa
     4381 gaccaggatt atcgggaaga tctttttcca gtcggccgat tagataaaga cacagagggc
     4441 ctcttgattt taacgaatga cggggtgttt gcccatcagt tactatcacc taaaaaacat
     4501 gtggagaaag aatatctagc tgaggttagc ggtataatga cagaagaaga tatccgttta
     4561 tttgcagaag gactgattat tgatggcgat gagcagacat tgccggcaaa actccaaatt
     4621 gatcaagtag atcaagagac agaaacttca attatccgtc tgattttgca tgaaggaaag
     4681 tttcaccaag ttaagcgaat ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga
     4741 atccgtatgg gaaaattttg gctgcctaaa gatttgaata aagggcaata tcgacccatg
     4801 accaaagaag agctagaaca agtgagagaa aaataattga atggatagct aaaagaataa
     4861 gcagagtagt tgtagcgcca tctgaaaagc agacttgtcc gtctgacttt cggatggctt
     4921 ttttgtgaaa tttcggctta tttccgtaga agctacttct ggaacaccgt ttgttcggaa
     4981 taagggactg tgacaagact tttgttacag tccccattgt tccttgtgac tcagagttag
     5041 atagtaagga cacgatctcc ctaatactca ggccaaaacg ctgaattcac aacctctgat
     5101 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     5161 ctattttccc gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct
     5221 atactttgtt tcattttccc tatactttgt ttatttattc tcattattga aagcgctata
     5281 attacgttgc gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca
     5341 tttttttatg aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct
     5401 ttggatgatt tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt
     5461 tcatatttca gcagatgggt tcttggctag cttgagggaa agtccttggg tgggttggga
     5521 aaatttcaaa tttttatttt cttcggatca agcatttctc atcactaaaa atacaatttt
     5581 atataatgta gcatttatcc ttttgaatct gctgatctca gtcgtatttg caatcattat
     5641 gagcgagttg agaaataaac gactcgttaa agtttatcaa acgatgtcct tactacctta
     5701 ttttctatct tgggtcatta tcagttactt tgtttacgct tttttaagtc ctgacaaagg
     5761 aatattcaat cagtggatca ctggacatgg aggagaagct gtcaattggt ataatgaacc
     5821 gaaatattgg ccgtttatct tggtgttcat tggtacatgg aaagggattg gttataacag
     5881 cattatttac tttgcatctg ttatgggaat cgatccgact tattatgaag ctgctatggt
     5941 tgatggagct agcaaatggc agcagatcaa acatgtgacg ataccacaac ttattccact
     6001 aatgacgatt ttaacgattt tagccgtagg aaacattttc cgagctgatt ttggcctgtt
     6061 ttataacatt cctaggaatt ccggtgcatt atatgatgtg actcaagttt tggatacgta
     6121 tatctacaat ggcctgacat ccacaggaga ttttggtatg acggctgctg caggacttta
     6181 ccaatcagtt gtcggctttg tcttgttgat gatcacaaat acgatcgccc gacgttttga
     6241 tagcgattct gcattattct aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc
     6301 caagtccgct ctttcaataa gacggctaat tttttctcta atataatgat tgcactcttt
     6361 gctatttcct gtattttgcc atttatattt gttatcgcca tctcttttac cgatgagtcc
     6421 atattgtcga ctcacgggta cagtttttgg ccagaaaagt tcagtatatt tggttatacg
     6481 tacttgttcg accagatgca ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt
     6541 actgttttag gtacactgat caatagcacg gctacttctt tatatgctta tgcgatttca
     6601 cggtcaaact ttccattccg tcgttttttt accgtttttt gtttaattac gatgttgttc
     6661 tcaccaggga tggtggccaa ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg
     6721 atttgggcat tgatcttgcc aatggctgtc agtcccttca atattattgt gatgcgcaca
     6781 ttctttaaac gttcggtctc tgattcgatt attgaatcgg cacggatcga tggagctagc
     6841 gaactgcgaa tatttgttca gatcgtttta ccgttagctg ttccaggtat tgcgacaatc
     6901 agtttatttg ccgcattggg ttactggaat gactggttca atgctttact atacatccaa
     6961 gatgataagc tagttccact acagtatctt ttgatgaaaa tccaaagtaa tattcaatat
     7021 ctgacacaaa attcaggagc aggaagccaa ttgacaggcg gattagcctc agttccagga
     7081 gaatctgcca gaatggcgat cgtagtcatc tcgacgttgc ctattgcgat cagctatcca
     7141 tttttccaga agcattttgt caaaggtcta acaatcggcg gagtaaaaga ataaagaaac
     7201 aaaaatataa atcataaaat taggaggaag aaaaatgaag ttatggaaga aactagcctt
     7261 tacaggtgtt tcagcgttgt tgttgggaac attagcagca tgcggaggtt ctggttctaa
     7321 agaccaggca gaagctagca attcggacac tttgcagatg tatcaaatcg gagataaacc
     7381 agataatttc gatcaattga tggaagtagc aaacaaacga atcgaaaaag agatcggtgt
     7441 aaaagtcaat atcaattaca ttggctgggg cgattacgaa aagaaaatga acgtcatcat
     7501 ttcttccggg gaaaattatg atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa
     7561 aggagccttt gctgatttga ctgaattagc gcctaagtac gctgaaaaag cttataatga
     7621 tttagatgaa gcatatatta aaggaaatct ggtcaatgga aaattatatg ctttcccagt
     7681 caatggaaat gtctttgctc aacaagtact tacttttaac aaagctttac tagataaata
     7741 cgatctttca attgatggaa tccaatctta cgctgatgca gaaaaagtct tgaaggaatt
     7801 ccatgaaaaa gaaccaaaca cggcagcttt tgccattgga caaggcttca aagtccaagg
     7861 gaactttgat tatcctttag ggaatacgtt accatttgct attgatctgg atggagatga
     7921 ttcgaaaatc atcaaccaat acgataatga aacgtatatc ggactgttac gtacaatgca
     7981 tagctggtac aaacaaggac tcattccaag tgacgctgca acgagcaata aggattatcc
     8041 actagaagga aatacttggc taatgcgtgg agaaacacaa ggaccgtatg attatggcga
     8101 tacaattttg acaaatgcag caaaacaaga acttgtctca aaagcaatta ctgttccact
     8161 aaaatctaca gctcaagcac aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa
     8221 agaaaaatca gtagaacttc tagggctgct taacagtgat ccagaattat tgaatgggct
     8281 tgtttggggg atcgagggag aagcatggga aaaagtcgat ggttcagatg ataaaatcaa
     8341 attgctagat ggttatcagc caaatacaca tatgtctgct tggaatacag gaaacaacaa
     8401 gattctatat acacaagagt ctattacgga tgacatgatt gccaaacgtg atcaatcgat
     8461 tgctgatgca gaaacttcac cgattttagg attcagcttc aacactgaca gtgtcaaaac
     8521 agaattaagt aatatttcta atgtcatgaa tcaatattta gatggattga atacagggac
     8581 tgttgatcca gatgaaacat taccgaaatt gaaagacgct ttggacaagg ctggctatga
     8641 taaggtattg aaagaaatgc aaaaacaata tgatgagttc cgacaagaat aataatgaat
     8701 aaagacaaag gggttgtgac aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt
     8761 tcaaaagcca gtttctcggt attaagctaa atatatgtaa aaatgtggaa agccatattc
     8821 acaaaatttt tcttactact cgaaactaag cggctatttc acaaactctt ggtaaacggt
     8881 gttcaatcag ctaaccctcg gtattcgctc acaaaacgac aaaatatgag gaactatttt
     8941 tccgtttgtt tctctaataa tcaggtcaag gtgctgattg cccaaccttt ttttgtataa
     9001 tgagaaagta taaacttaat gtttggagga gaagatatgt tagggaaggt tttggaagag
     9061 ttatttatcc ggatctgggt cattataaaa ctaaccttgt atttctggat atatacattt
     9121 gccggcggga tcatctttgg attaggtgcg gcatggaaaa cagtcaatga actattttat
     9181 ttgtacggat tcgagtataa agaaatcaca ataaaaagag gctggaatat atataaaaga
     9241 aattttttaa gaggcaatct gctatttagt ttatttttat ctggtactgc tttattgagt
     9301 tacaatttat atttatctgt tcaaatcaaa gggctcattt ttttagctat tgattttatt
     9361 ttactttttg ccttgctctg cctatttgct acttatcaat acagtatgat tttagatagt
     9421 gaatatacga tctctgttcc taatctgcta aagctaagtt ttatttcggt tttttccagc
     9481 ttttcgtctt ttctaaaaat aattattggt agcggtataa ttttaggcgt gacttggcaa
     9541 ttcaaaggat tgatcctgtt tggtgtcatt ggtcttttga cggtttggaa tggtacgatg
     9601 acaacacatt ggcgtgaaga attagataaa cagttggaaa gttatgaata agtttcggat
     9661 tctattttca aaaggaacct tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat
     9721 cggtgtaacg atgactgcta ccgttatttt tagtatccat acttgggaac aaaatcaaaa
     9781 acaggcagaa aacatgacaa gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa
     9841 aacaaccttg agtaggatca tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa
     9901 tgtgacgacc tacttaacaa aacccataga tcagtatctg atgtatgtat acgaacagca
     9961 aaacagtacc gatgaattag tttctttccc aaatcaaatc aaagatctct acgcaaatta
    10021 tgaagaacta agtgcgatat atatcgtgtt gaatcaacta ccggagtatt atgaatcttc
    10081 tagagaaaac aaaggtgggg aaataaaaaa ggggacacca aaattactag acgcctttta
    10141 tatcaagttg cctatcacac aaggaggagc tgagtcagga agtattttta ttggttttca
    10201 aaagaaagaa ctagatatga ttttagaaaa tctaacgtca ttcaatggac tctctttata
    10261 catgatttct ggaacgacaa atcgactata cacattccat gataataaaa tagctaaaga
    10321 aacgtttgaa agacaagaaa tgatcatcac agagagtctg aagaaaaatt cacaattgcc
    10381 gattgacgat ctggaaaaaa acaattttgt tcaacaccaa gaactgtctg atgattatcg
    10441 tatattagct gtactagata aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc
    10501 gctgatcatt ggtatcatta ttttagacaa tatactgctt atctttcttt ataaaacatt
    10561 caaacgatat tcacagcaag tatctatcgt catgcaggcc atggatcaaa cagcagcagg
    10621 gaatttagag acgcggatcg acataacaaa cacggaatat gaactgaagg aactttcaat
    10681 cggtatcaac gaaatgttgg atagcatcaa tcagtttgta gaagacatct acaaattaga
    10741 aatcaaacag caagacgcac atatgcgggc gcttcaagca cagatcaatc cacacttttt
    10801 atataataca ttagaatata tccgaatgta tgcaatcagc gaaggaagtg aagaattagc
    10861 agatgttgtg tatgcgtttt ccgctttgct tcgaaacaat acgaatcaag agaaaacgat
    10921 cactttaaaa gaagaactgg acttttgcga aaaatatgtc tatttgtatc aaatgcgtta
    10981 tcctaatcga gtagcctatc attttatgat cgatcctgat ttggaaaaga tcgaagtacc
    11041 taaatttgtg atccagccat tagttgaaaa ttattttaaa cacggtattg attttactcg
    11101 attcgataat gcgctaagtg tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat
    11161 caaagataat ggaaaaggga tgactgaaaa acggctaaaa cagattgaag aaaaactgtc
    11221 tcatcctaaa gtggaacttc acggttctat cggtctgcaa aacgtgaatg aacgtttacg
    11281 tgccagcttc ggcagcagtt attatatgtc gcttgagaat aatgagacag gtggactgac
    11341 cgtttctatt acattcaagg agggatgagc gtgtatcaag tgttattggt agatgatgaa
    11401 tacatgattt tgaatgggtt aaaaaagata attgattggc aaagtttagg ctttcaaata
    11461 gtagcaacag cagagaatgc caaagaagga ctagctgtac ttgaacagcg acagattgat
    11521 ttagtagtaa cggatgtgac aatgccagaa ataaatggac tggaatttat tgaagcggct
    11581 caaaaagaac gtcataattt tgaatttatg attttatcag gataccaaga attcgattat
    11641 ttgaaaggag gtatgcaatt aggcgccgtg aattatttga tgaaacccgt gaataagttc
    11701 gaattggttg aaagcctcaa gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa
    11761 aatcaacaag aaatctatca agaactgtta ttcagtcaat ggttgaatga agaattggat
    11821 gaaataagtg aagaagaaat aataggacaa ataagtgaga agcagcatag gatagtattg
    11881 attcaactgg caagaactca tgatactctt atcaatagat ggttgcaaga acggcaacaa
    11941 atcttttact atcaacgaaa ttacggagac cgtgtattga tcacgttgtt gttggaggca
    12001 gatgaagttg atggcttctg ttgttttgtt caagaaaact ttcctgatca ggaatggctg
    12061 atcagtatcg gggaagagac agattctttg gacaaaatcc ctgaaacatt ccaacaagcg
    12121 aaagacaatc tacagttgca tcaattttat ggagataaag acgagcatgt tttttacgca
    12181 gtacaagcag aaacaaaaga tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa
    12241 aataaacaat tggaagtcgc tcaaaaaatg atcactgatt tttttgaaca gtttcagtta
    12301 gcagccatga tgccggaaga tatacgatat tccagttttt tactgtttat ggagattcaa
    12361 cgggaattga ttgatctgga agatgaggaa tatttacaag gaatcgaaaa aatccatcag
    12421 gcaaaaacgg tgcaagaact tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga
    12481 cagaaaaagt acagtgacaa tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa
    12541 gaaccactta cactcaaaga agtgtcagaa aacctgcatt taaatgtcat gtatctagga
    12601 cagctgttca aaaaagaaac gaaaaaaagt ttttcagctt atttgaacca tttacgtatg
    12661 gaaaaagcga aacaactgct tttgcacagt aatcaaaaca tcaatgagat agcaagtgag
    12721 attggctaca acaacaccac gtatttctct aaattattca agaaaatcgt cggcagatcc
    12781 cctaaagagt accgagaaaa cagtggagaa tcataaaaaa aaaagccaat gtaatgaaat
    12841 aacttataaa aaattgtcta gaaatctaat tttgggctgt tcttattact tgaggcaagg
    12901 cagctttttc atgacctctc gataaacgga gttcaaaagc cagtttctcg gtattaagcc
    12961 aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
    13021 aacagctttt tcacgacctc tccactaaat ggtgttcaat cagctgaccc tcggtattag
    13081 ctcaaaaaat ggcaaaatat gaggaactat tttgccattt gtctctctaa tactcaggtc
    13141 agaacgctga tttcacaacc tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat
    13201 aagaccaacc aaacgaaaaa tatgaggatc tatttttgct tggctgttct tattacttga
    13261 tgcaggacag ctttttcaca acctcttgtt aattatagtt tgataaggaa aaaggcaaat
    13321 cttttgcttg atatttctta gggtgaggga tgatccctaa ttggtaagag atcgtgccgc
    13381 ctttcagcaa atcttgatgg gtaaaatata atttcgtata ttctctgtcc atccgtttga
    13441 ttttatggat aaattgttgc tgcggccgat taggcgttgc ttcaatcgtc aattcctttc
    13501 catttgacaa aagcaaaaca gccttgtcaa ttaatggcat accgataaca tattcaccgg
    13561 ttccaggcgt gacaggataa aatcccagac tattgaagat ataccaagcg gccatacttc
    13621 cattgtcctc atctccaggg taacccattg gagaatcgtc gaaggtctga gtcatcaact
    13681 gtttaagcag tggctgtgcc atttcaggtt gtcctatata actgaacaag taaggatagt
    13741 gaaaacttgg ctggtttgaa atagctaatt gtccaaattc tatcgcggcc atctcactca
    13801 tttcgtggat ctcaaaacca tatcctcctg ggtcaaacgt tgggggctga ttgcacagct
    13861 caatgagttt ttctttatat ctctcactgc caccatattt tccgatcagt cctgaaaaat
    13921 cgtgataaac agcaaagctg ctttgccaag cacttccttc tgcataatct tgtccccaac
    13981 gattagctga aaatggttct ctgaacttgc catctttatc ttttgaacgc ataaatcctg
    14041 actcatagtc aaagatattt tgatagttca atgattgctg ctgatatttc ttcgtcattt
    14101 cctcttcttc tagtaccttg gcaacttggc taatacaaaa atcgctataa gcatagtcta
    14161 atgtgtgatt gactgattcg tggtagtgat taggaacata gccatatgtc aagtaatctt
    14221 ctgttccttg gcgtccataa tttggatgtt tgctttgaac cgtagcacct ttttccattg
    14281 ccgttagaaa ttcaggcatc aagtctcgtc ggatatcttt tacagcagca tctgcaatga
    14341 ccgcatcgat caatgtccca ggcataagtc cacgttcatc aggagacagc cattttggca
    14401 aatagccact ttctctatag ctgttcaaaa aaccttccag catttcttcg tatttttcta
    14461 cagcaatcaa agaataaaga ggataaaccg ttttatacgt atcccaaaac ccattatttg
    14521 tatagagaac accaggtttt atagatcgac tagtcgtatc atagtggatt ttttgcccac
    14581 taggatcaat ttcataaaat gtttgtggaa ataaaaacac ccgatataaa ttatgataaa
    14641 acgtccgcaa ttgttgttga tcatggtgtg tgagctgtat acgatcaaaa taggactgcc
    14701 attgttcttg actgtgagca aggtaagtct ctttgctttg gttttgttct agttccaggt
    14761 tataagctgc ttgttccgag ctgataaaag atgtcccaaa tcggatagtt tgtttttttg
    14821 tctgttcaaa atacaacggg atcgagtcgc tatttttgtt ggaaacagct aactcatcaa
    14881 ttgttaaagg atattcaaat ctcaatataa aataaaaaga aaaatcagga tcttctgaac
    14941 cagcaaaatg tgtgattttt ccttccactg tgtaaggatc tataacaaag agctgatggc
    15001 gtcccggaaa ggataaaagt aaaccgctgt catttttgct gtaatcaata gataagattc
    15061 cgccatacat gctaggtatc aatgtagaac agatgccata acgtaattga cagatcgaca
    15121 gatgagtcgg gcaaaatata ctttcttctg ggcggtaaga actttgagca tggaaaaggg
    15181 tgttctcctg tagttttcca ttgatcggcg taaacagcat atgactgaaa tcacccatcc
    15241 aaggacttgg ttgatgggta agacggtagc cttgaaaaac gcggtcatca ggatgaaacc
    15301 accagctgcc gttttgatcc gtagtttgcg gtgcaaaata attcatccca aaaggaacgc
    15361 cagtataagg ctggcagttc ccattagaga aatttggctg attcgctgtt gcatgtctag
    15421 tatctatttg accgatattc attttatttc tcctttgttg attgtctttt gatgaatgaa
    15481 actggaaatt tactatcgga tggtcgatct tttgtctcta accaagtgac caaagcatct
    15541 cccgcaagct tacctaactg ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca
    15601 agagctgctg cttggatatc atcaaatccg ataattgaaa aatcttcagg gatatgataa
    15661 ccatgctgtc tcaagagatt catggtctgg atagcgacaa catcgttttc acaaacaaaa
    15721 gcagttactt catacttagt gatataagaa agcaattcat ctaatgtcgc gcttttgtca
    15781 tccagtgaag tgtggaaagt caaaccagct ttgtccaatg cttcaaggta accaaggtaa
    15841 cgctgtcgaa cggattgtgg atgagtctgc gtaccaaaca aataagcgat ttttgaatgt
    15901 ccctgttcga tcaaataaga ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg
    15961 gtaggaaaag gtaactcata gattttttta tctaatatga cgacagggaa ttctttcaat
    16021 gaaagctcaa aaagtaagtc aagatgttgt tctgtatcaa gagcataata aatcaatccg
    16081 tcaaattcat tgattagctc ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc
    16141 atgacatcaa tctgtttatc ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca
    16201 gacaaatcag cgacatatgg aagaagaaac agaatccgtc gtgtttttgt aggagaagca
    16261 agaatctgtt ccggagaagc ggatttaacg aaactgcctt tcccgcgaac gcgataaata
    16321 agtcctaatt gctctaattc agtcaatgca cgtttggaag tgattctgct tactttatag
    16381 gtacgtgaaa gttctttttc agttggtagt tggctgtcta caggtaattc accagttaaa
    16441 atagccgttt gcaaatcatt aaatattgtc tggtacaatg gttgagtcat ataaacacct
    16501 cttaatgata tatcatattc aaataatacc ataatggatg aaaaagtaaa taaaaagata
    16561 ttttttgaaa gcgtagacat aattggctgt atgaagtata atctttaaag atatatcaaa
    16621 tagaaagaag gttttattag tggtttataa agatatacca aaatcggtgc agaaatttat
    16681 ggaagatatc acacaaaaat gtggggatat ccatgcggac tgggcaaaga attttacagc
    16741 agcatttgcc aatactctgt tgacaacagt caaaaagcaa aatgacggaa ccaccttttt
    16801 attgacggga gacattccag cgatgtggtt aagagactct actgcacaag ttcgtcctta
    16861 tttagtgatt gcaaaagaag atgaggaatt agctgacatg atcagcgggt tagtcaaaaa
    16921 gcaatttttt tatatcaata tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg
    16981 tgctggccac caaacagatc atacaaaaat gaacgattgg atctgggaaa gaaaatatga
    17041 aattgattct ttatgctacc caatacagtt ggcctatctt ctttacaaaa atacaggaaa
    17101 aactgatcaa ttcaatcaat cttttattga aggtatcaaa aaaatcttgg acgtgttcaa
    17161 aacagagcaa gatcatgaac actctcctta tacatttgta cgagacacga caagaaaaga
    17221 agatacgctg atcaatgacg ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc
    17281 tggtttccgt cctagcgatg atgcttgccg ttatggatat ctggtaccat ccaatatgtt
    17341 tgcggtggtc gtgttaggtt atgtggaaga aatttttcaa acgatactcg atgataaaga
    17401 aattgtacag caaacacaga aattgaagaa agagatccaa gaggggattc agaatcacgg
    17461 acgaacggtt aatcaaaaag ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa
    17521 cgccacagtc atggatgaca gtaatgtgcc caatctgatc gccgcacctt acttgggata
    17581 ttgttctgaa caagaggaag attatctgca aacaagaaga acattgttga gtaaagaaaa
    17641 tccatatttc tatgaaggaa agtacgcaaa aggaattgga tcctcccata cgccagaaaa
    17701 atacgtttgg ccgattgcat tagctatgga aggaatgaca acgtcagaca aatctgaaaa
    17761 agagcggata ttgaatttac ttgtagcaac tgacgcagga acacacctta tgcacgaagg
    17821 attcgatgtg aacaatcctg aaaattatac gagagaatgg ttttcttggg caaatatgat
    17881 gttttgcgaa ttagtcatgg attactttga tattagagta gaaaagtagg aggtatcgta
    17941 aatgaaaaag aaagtatata tcatttctca tagccactgg gatcgtgaat ggtacatggc
    18001 ttacgaacag catcacatgc gactgattga attgatagac gatctactag agctatttga
    18061 agttgatccg tccttcaaca gttttcattt ggatgggcaa acgattattt tagatgacta
    18121 tcttcaagta aggccagaaa aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt
    18181 gagaattgga ccattttata tcttgcaaga tgattttctt atcagctcgg aatcgaacgt
    18241 gagaaatatg ctgattggca tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg
    18301 ctattttccc gatacatttg gaaatatggg acagacgcct caattaatga aacaagctgg
    18361 tatttcagca gcagcgtttg gccgtggtgt aaaaccaatt ggctttgata atcaagtttt
    18421 ggaagcagaa aactattctt cccaatactc agagatgtgg tggaaaggcc ctgatcaaac
    18481 agcaatcttt ggtctgctat ttgccaattg gtatagcaac gggaatgaga ttccagtaga
    18541 aaaagaagca gctcttgcct tttggaagca aaaattagcg gatgcagaac aatatgcctc
    18601 tacgaaccac ttattgatga tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc
    18661 aaaagcaatc catctcgcaa atgaactgtt tccagattac gagtttattc attctaattt
    18721 tactgactat ctggaagcag ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg
    18781 agaattgaca agtcaagaaa cggatggctg gtatacttta gccaatacag catctgctcg
    18841 ggtttactta aaacaatgga ataccaaggt ccagcgacag ctagaaaata ttacagaacc
    18901 acttgcgacg atggcttatg aagttagtgg aaactatccc catgatcaat tagattatgc
    18961 ttggaagaca ttaatgcaaa atcatcctca cgacagtatt tgcggttgtt cagtcgattc
    19021 tgtccatcgg gaaatgatac ctcgttttga aaaagcagat gaagttggaa agtatcttgc
    19081 acaagattcc ttagaacagc tgaccgcagc aatcgatacg accggattcc caaaagacag
    19141 ctttccattt gtcattgtaa atactgcagg aatggataaa actggtgaag cagaaataac
    19201 catcgaatta gaaagaaaaa gattcgctga aggaatacct gaacaactat atcaagagtt
    19261 agaaaatttg cctaaacgta agtatcatgt agaaacaaaa agtggggcca ctataccagc
    19321 tattttatct gaagaaactg tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt
    19381 gccatatatg gcaagaatga tcaaagtgac tttaccatta gaaaacatgc cagcattttc
    19441 ttgggaaaca ttcgcgcttg tagaagggga agcgaaagca gaagaaaagg aaacaatgat
    19501 tcatcagagt ggacgaatca tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg
    19561 taccattaca atggaagatc gcaaaaacaa acgaaagttg aataatctgc atatttttga
    19621 agatatcggc gatataggaa atgagtatat ctttaaacaa cctttctgtg atcagccaat
    19681 cctatccagt aacaaagaaa actctgaagt aaaagtatta gtagatacac cggaaatagc
    19741 aaaaataagt attctacagg aaatggaaat ccctgtttct gcagacgagc gtttagaaaa
    19801 agaacagcag atggttgtgg aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg
    19861 aatccttcaa ataaaaacaa tcatgacaat cagaaaagat agtaaaaaaa tcgactttga
    19921 aacgactctc gacaatcaga tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt
    19981 acatgtggaa catcacgaag cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt
    20041 gtctaaaagt tgggaaaacc ccacgaatcc gcaacatcag caagcatttg tcaatattca
    20101 tgatgaagag tacggtgtaa cagtcggaaa ctttggtttg aatgaatatg aagtaacaga
    20161 agacggacaa atcgcagtga cgctacttcg cagtgtggga gaactaggcg attggggata
    20221 ttttccaaca ccagaagcac agtgtctagg tgagcatcgg ttcaattaca gcattgaact
    20281 acatggacca gaagaaaaat tctctactta tttgcatgca tacgcagcac agattccttt
    20341 ttccactcaa caaatcaaac atcatgaagg aacattgatt tcaaaacaac aatatttaac
    20401 gataaaaagt gaaacgtttg caattacagc tttgaaacgc agcaagttta gtgataaagt
    20461 agtagtaaga ggtttcaata tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa
    20521 tggaaaaact gtcatattga atctgcttga agaaccaacg aagcaagcgg ttgtcccaat
    20581 aatccaacct tatgagattc ggacaatcgg attcgaggag gaaaacgaat gtatggcgga
    20641 atagaagcag gcggaacaaa atttgtgtgt gcggtttcag atggtggaga aattttagaa
    20701 aaattaagta tccctaccac aacaccagaa gaaacattgg atcaggtatt tgaatttttt
    20761 gatcgttatg aattgaaggg aatcgggatt ggctcattcg gcccgattgg tatcgacaaa
    20821 acaaaagata attacggata cgttttggca actcccaaaa aagggtgggc acaattcgat
    20881 tttcttggca caatcaagga acgctacaat attcctgctg cttggacaac ggatgtcaat
    20941 gctgctgcat atggggaatt gaagcaagga gcagcacaag gaaaaaatag ttgtatttac
    21001 cttaccgtgg gaacaggaat cggtgcaggt gtcgtgatca atgaagaaat tttttcaggg
    21061 atcgcacatc ctgaaatggg acatatttgg gtgaaacgcc atccagatga ccattatgaa
    21121 ggtacttgtc cttatcataa agattgttta gaaggattag cagcaggacc gtctattgag
    21181 gcacgcacag ggatcaaagg gcaagatctg ccgcaagatc atcctgtttg ggctattcag
    21241 gcttattata ttgctcaagc actgatcaac tatacgttga cgttagcacc tgaaaagatt
    21301 attttaggcg gtggggtaat gaatcaggat catctccttc aaaaaatccg tcagcaattt
    21361 gttgaattga tgggtggtta tatggaaaca cctcaagtaa gtgaatatat cgttcgttgg
    21421 ggactgccga atgaaagtgg gattatcggt agtttgctgt tagcagaaca cgaacatcaa
    21481 aagtaagcag acaaattaca caaaaagaac agagtcttat cattatctgc tgataagact
    21541 ctgttctttt ttatttctat tcccataatc caggtaaagc tttaccgact attttttctt
    21601 gagtaaatgg atggatcatg tgcagttcta atgcatggag catcagtcgt tcagctgttc
    21661 tattttggta gagagggtct ccaacgatcg gatgtccctt atgagctaaa tgaactctga
    21721 tctggtgtgt acgtccggtt tccaaaacac aatagacttg tgtttgttca gaaagtactt
    21781 tgttgacagt gacatgtgtc aaggcgctct gaccattttt atcatctacg accctttttc
    21841 tccgatcatg gcgatctcgt ccaattttat tggtgatggt catatctttg gcaatttctc
    21901 cccatacgat ggcttgatat ctccgataga tcattttttg ttccaacatt cttccaagta
    21961 tcggcaagac aaatggattt tttgcaaata aaacggcacc gcttgtttct ttgtccaagc
    22021 ggtggacaac gtaaggtcgt tgctgtttag tgtcgagata agcagctaaa tgattgagta
    22081 aggtactgtt ttctgtcggt tcatttggat gagtcttcat tcctgcaggc ttatccacta
    22141 taatcaaatg ctcatcttca tataaaacac ggatgtgctt cgcatctcct aattgaactt
    22201 cttgataatt ataatctgtt tcttcgaata acaatgtgat ctggtcattg ccctttactt
    22261 catgatggaa caaagcaggt tgttgattga tccaaacgtt tttacgtata cgcaaaaagt
    22321 gtcgaacctt tctaggaact aaccattcct tttcaagcaa gtcgcgaatt gttgtttcag
    22381 gatggttcat aggtaaagtg atcgtgattt tcacaagtgt actcctttca gtgcaatcgc
    22441 agactaattg taccattttt aaaacatatt taagaaaaaa cttacatttt tctgttcatt
    22501 cttatgttac tctacttgca ctaacgtaaa aaaacatatt gaactacaac ttaagcagga
    22561 agaaaacact ctttcactat ggtaaaataa gacgattgaa tcaaatagac aggagaagaa
    22621 gcaaatggat tttcaaagca ttttagaaaa gataaaagct gctttgatca gtttttggcg
    22681 ttggatcaga ccatatctcg gacaattcca tcgatggcga aagcgcatct ggaaaaaata
    22741 tcatgtgaac aaattgattt tgctgattgg tttgatctgt gtcttagtga caagtatcta
    22801 cctctttatt ttagcaaaac aagcaaatgt cgaaacgtta aaatcaggat taagccaatc
    22861 gacagtcatt tatgataaga aaaacgaaga agctggcaca ctgtacgccc aaaaaggaac
    22921 atatgtagaa ttaaacgcga tttctcccta tatccaagat gcagtaatct cgacagaaga
    22981 taggaatttt tacgagcatc acgggtttga tatcaaaggg attgcccgtg cagcagtccg
    23041 aatggtcttg tctggcggaa catcaggggg cggaggaagt acgatcactc agcagcttgc
    23101 caaaaatgcg tatctgacat tagaccaaac gtttgatcga aaagcgaagg aattattttt
    23161 ggcaatcgag atcgaaaaga agtacagcaa agaagaaatc ttgaccatgt atcttaacaa
    23221 tgcctatttt ggcaacgggg tatggggtgt ccaagacgct tccagaaagt attttggagt
    23281 agatgcaacg aatgttacgc tgtctgaggc tgctactttg gcaggaatgc tgaaaggtcc
    23341 tggtatttat aatccgatcg atcacccaaa aaatgcgaat gatcggaaaa acacagtact
    23401 aagtgtaatg gaagaaaacg gaaaaatcac aaaagaacaa gaacaagcag aaagccagac
    23461 agatatcagc gcgtatctta atgacacata cgataattca gattctggtt atcgttatcc
    23521 ttattatttt gatgcagtga tcgatgaagc agttaacgag tatggtcttg atgaagaaga
    23581 tgtcatgaat aaaggctata aaatctatac agctttagat caggattatc aaaagcaatt
    23641 agaagctact tatcaaaaca gtgcattatt cccagcaaat gcttcagatg gtgccatggt
    23701 ccaatctgct tcagtagctt tagatccgaa tacaggtggt gtacaagcac tagtaggacg
    23761 aagaggagat catgtcttta gaggattcag ctttgctaca cagatgcgtc gttcaccagg
    23821 atcaacgatc aaaccattaa gcgtatatac accagcactg gaagctggat acaagccttc
    23881 cagtatcttg aaagacgaac cgcaaagtta ttacgatgcg cataattttg acgggacata
    23941 ccaaggagaa gtaccgatgt atcaagctgt tgcacaaagt ttgaatttgc ctactgtatg
    24001 gcttttgcat gagatcggtt tacaaaaagg atatgataaa gcagaagaat tcggcctgcc
    24061 tttagcaaaa tctgataagt attatggctt agcccttggt ggactggaaa aaggagtatc
    24121 tccattgatc atggcgggtg cttacagtgc atttgccaat gatggtcaga tgtatacgcc
    24181 gcatcttatc acgaaaatcg ttgattcgac tggagcggtg atcgtcgata acacaaacaa
    24241 aaaaccaaaa caggtcatat caaaggaaac agccgatgaa atgaccagca tgcttcttgg
    24301 tacattttca aatggaacag cggctgctgc gaatcctgca ggttatacga tagcagggaa
    24361 aacaggaacc acagaaacta atttcgatgc cacaaaagca aatgaccaat ggatgatcgg
    24421 ttacacgcca gacgtagtga tttcgacatg gatgggttac gaagaatcca gcaagctgca
    24481 ctatttggaa gggacaagtg gaaatgttgt cgggaaagtc ttcaaatcag cggcagaagg
    24541 catccttccg tatacttcaa aaacaggatt tagtgtagca gatgcctatg ccacaggagg
    24601 acaagtggta gctgcagatc aagttggaaa cacaggaaat tctaatggag agtctggaaa
    24661 ttggcaagat aacttgaatc aatatggtga acaagcaaaa gaagggctga aaaacttcgg
    24721 cgatatggtg aaagaaggcg ttcagggaat tggtgaagcg gctaaagacc tctggcggaa
    24781 atatcaagga gagtagcccc tttgaaaacg aataacccca tgttacaata gagagtaact
    24841 actaaaagaa aagagggcat aacatgtcaa acatctacga tagcgcgaat caaatcgaac
    24901 gtgagatccg tgaattacca gaattcaaag cgttgaaaga agcgtacgag aaagtacaag
    24961 caaacgaaga agcacacaaa ctgttcaaag acttccaagc gatgcaaatc gaattacaag
    25021 aaaaacaaat gagtggacaa gagttcagcg acgaagatgc aatgaaagca caagaaatgg
    25081 ctgtgaaaat ccaatcagaa gaagtaatca atgacttgat gcaaaaagag caaggcttca
    25141 gtacaatcat caatgacttg aaccgtatca tcatgacacc agttcgcgac ctttacagcg
    25201 aataaaacta ttttatgaaa caacagaggg tgtgaaagag acgttcggcc ttgagcgatg
    25261 acaaaattgc ttcttattgc cgataggctg tctcttgaac accatttacc aagaggtcgt
    25321 gagcctaccg tttaactctg agttacaagg aacaatttca aaaaacagct tctcatgttt
    25381 gatgactatt gtgacttgtg accgaggagt tggcaggaga acaccgttta ttcagaaata
    25441 ggagctacga caaaagtctt gtcatagctc ctattttttg tcattcattt ttcaagcggt
    25501 agaaatagcc tttttgagct aacagttttt cgtaagtacc tatttcaacg atttttcctt
    25561 catcgatcac aacgatttta tctgcctctt tgatcgttga aagccgatga gccacaataa
    25621 aagtagtggg tgtttctgtc aggtaattca gggcttgctg gatttttttc tctgactggt
    25681 tatctaaagc tgaggttgct tcatctagca aaatgatttt tggctgacgt atcaacgctc
    25741 gtgcaatgga gatacgttgc ttttggccac cagacagctt gttgccatgt tcgcctacta
    25801 aggtatccag accatcagga agctggtcaa tcagtgaaga aagttgcgca gcttcgatga
    25861 cttcatctaa acgttctttt gatacgtttt tcaagccata agtgatgttc tctttgatcg
    25921 aagcggagaa caacaaagtg gtttgaggaa caacggctaa ataatttcgc acacttcgca
    25981 tatccagttc atccaaaggc tgatcatcta gaaaaatttt tcctgaagtc ggctggataa
    26041 atccaatcaa taagttaagg agtgtagatt ttccagagcc agatggccca acaaaagcaa
    26101 ttgtttctcc agctttcacc tctagagaaa aatgatcgat aatcggtttg agtgagtccg
    26161 gataaagaaa agaaacatcc tcaaaacgaa aatttccatc caaatgttgg atttttttct
    26221 taccgtgata agtttctaca tcgccagcaa tcaacagttc gccaatagaa gtgatagatt
    26281 ccatgccttt tgaaatcgta ggaatcagcg tgatcattcc agaaacggaa ttgacgatcg
    26341 tcgtgaaata cgtctgatac atcacgacat cgccgggttg gatgcgccct tgtaaagcta
    26401 atatcccagt gaaaatcaaa caaatcagct gaaatatctg aaaactagcc cagctgactg
    26461 atccgaaaac agattgcagg atatctaagt ggaatccttt ttcatagatt tgagtaaaat
    26521 gcttatttaa gcgagttgtt tcgtgttttt ctaacccatg agctcgagca atcggtgcta
    26581 attcgatcac ttcgatcact tttgcagaag tctcttccat ttcttgcctg aagtctgagt
    26641 tggactgtct gatcctttta cggaaaaaga gagtaacgaa tgcggcgaga ggaacagtca
    26701 ataagaaaaa catgaaaaca acaggacttt tataaagcgt gatacctaaa gctacgaata
    26761 aattgacagc gatatttaat ccgctgacaa atacttgaga ggacaacgtt tggatagctt
    26821 ctacatcacg gatgatcttt gactggattc gtccagattg caattctttt tgataaggaa
    26881 tagatagttc ttggattttc ttgataaggg tcgctcttag tccagcttct acttgacgga
    26941 tcgaagtgct atggtatttt acatgaaaat aattcgtcaa tatattttga ccaatcagac
    27001 aagccatcaa aaaagcgttg aaataaatgg cctgcaactg agaagaatct ttcgatgaag
    27061 ctgcattgat tacattggca atagcgatcg gcattaacca agatggaaga tgtttgataa
    27121 taaaaaagag tcctgacatg gcaaaacgcc aataatttcc ctgataaaga tgcagcaatg
    27181 tctgaaaagc atttgcatga tatttttgat aacttttctg tattcgttcc tccataatct
    27241 tctccttaac aatcttggta aataaactgc aagtgttcta ttgcttcagg aacataaatc
    27301 tcaggagtag cttcaagagt ggcgtacatt agaggacgct catatttgat ataatgcagt
    27361 accgggcgga aatctaaaca gcccttgcca acaggtacca tgcggatctt tccattttcc
    27421 atgatatagt ctttgagatg aagcgctgta atataaccat gtaaattgtc caaggcacct
    27481 cggatcactt tttcctgatc agcatgattt tctttatgca tcaaattcgc acaatctaaa
    27541 atgattttta aatttggtga agctacttca tcgattaaac gtttagctaa ctgatagtta
    27601 tagattggat gattgatccc tgcttcaaca gcaacagtta cgccaaaatt ttctgcatga
    27661 gcgactaatt caatgataga atttctggcg atctgataag cttcttcagt aaaattttct
    27721 tttgtgtaac cgacagagac actaccggtt tccgtagcaa tcatcttggc ttgataggca
    27781 gagcacagag caatttgctg tttgaaagtt gccaatgctt gtgcacgaac ttcttgattt
    27841 ttgctggata ggttgatata gcatcctaaa atggaaagtt cgatattgtt tttttggaag
    27901 tattcaccaa aataggcagc actcccaagt gtcatgtcag ttaatttaag ttgttcagga
    27961 aatgcttttt gtggtgcaaa ttggatatgg gacaagtgaa gctgtgatac tttttgaacg
    28021 atttcttcac gatttcgaac atctaaatcg tgggcacgga tgcctaaatt taatggaaac
    28081 atctcaaact cctttttgta aataattagc aagaggcaga cgggattctt ttatcaattc
    28141 aacaattatt cgggcaactt tatcagcgcc ttctggacta aagtgggtat tgtcttttag
    28201 tccttctggg taatttttac aagtatcagg tataagatgc aggtagaatt tttttgtctc
    28261 ttcttttgag aaggtctgga acaattcttg tgatcttgag aacacatcta aaagaggaat
    28321 atggtgtttc tcagcaaatg cacgcatcgc ttttggatag tctccaagta tatctggatt
    28381 caatgttccg ttttctaagt aatctcgtcg cgtcacagat gtcagtaaga ccatttggac
    28441 gttcgctttt tttgcgcatt caaaaaatat ctgcagattt tttaaatagc taccgaaggg
    28501 aagcgtacct ctttcttctt ggattttctg gtcgttatgt ccaaattgta caaataagaa
    28561 atcattcggc tgaaactgtg aagctagacg ggctaacctt ccttcctgga taaatgattt
    28621 ggtgctgcgt ccattctttg cttggttatt gatctggatc tcgggtgaaa aataagcaga
    28681 gattttttct ccccaacctg tctcgggacg cttgttttca tctttttctg ccgcagtaga
    28741 atcaccggca atatggatag taggcatgtt atctcttctt tcataaaggt ttataggcaa
    28801 tgaagcagaa cagcaagaga agaagacgga gcaaatttgg tccgtcttct tctctaaaac
    28861 gtggataaac ggattccgtt atttcactat cctgtttttg attcgttact gttactatta
    28921 atcaattagg caaaaaagcc aaagtaattt ttagcatttg tataactaat cgcttgaact
    28981 agacttccta aaagttgttc atcttcaggt gcttctcctc gcatgaccat ttcgcctaaa
    29041 aattcacaaa gaacacgacg gaaatattca tgtctagtgt aggacaggaa acttcttgaa
    29101 tctgtcagca tgccgacaaa attccccaaa gcactaccat cagcaaattg tgtcaattga
    29161 tcgcgcatac cagctcgagt atcgttgtac caccatccag aacctaactg cagttttcca
    29221 ttctgttctt tttggaaaca gcccatcaaa gctaaaatcg caggatagtc attcggattc
    29281 aatgaataaa ggatcgtctt tggcaactga tccgtttctt ctgcacggtt caataatttt
    29341 tgcagaggaa tagtcaaaga aagatcattc attccatcat agccggtatc gggccctaat
    29401 ttttccaaca tttgtgtatt acagttacga taagcatgga tgtgcagctg catcgtccaa
    29461 ttatgagaat gatagaagtg ggtcaaatgt atcagtgttt ctgtacgata agcatcgatt
    29521 tcatctgaag ataatagttt gttagctcgt gctttttgaa aaatctcttc taatactgtt
    29581 tcatccgctt ctttatagcg taagatgtct aaagcgtggt cggaaacacg acatccatgt
    29641 tgatggaagt attctatccg ctgccctaag acttctatca gttcttgata ggtggtgatc
    29701 gtttggtcag ctgcttcttc cagtagtgtg acccatgcat taaatccttc tttttcaata
    29761 ttgagtgctt tatctggacg gaatgaaggt agaaccttca aatctggttc ttcatgagca
    29821 agcaactgat gatagtgcaa gtcgtctgcg ggatcatctg ttgtacaaat cacttctaca
    29881 ttcgattttt ttgcgatttc ccgacggcga aaatcaggag ctgccaaaat ttcatttgct
    29941 tgtgtccaaa ttttctctgc attttcttca tttatcaaca agtcaatacc gaagaaacgc
    30001 ttcaattcaa gatgtgacca actgtaaagc ggatttccga taatttttgg tacggtttga
    30061 caccaagcta aaaatttttc aaaatcagaa gcatttcctg tgattttttc ttcaggcacg
    30121 ccgcaagctc gcatcaagcg ccatttgtaa tgatcgcctc ctaaccaagc ttctgtcaga
    30181 ttgctgaaat tgttgttttc gtaaatttct tttggatcta agtgacagtg ataatcaata
    30241 atgggcatgt tttttgcgta ggaatggtaa agctttttcg cccaatcgtc ttttaataaa
    30301 aaatcatcag ataaaaacat aaatcttcct tctttctttt tatactgttt tatgaaaatc
    30361 aaagtgttac tccgttttta aaaatggcaa tttctcgaac gtcattttgt tcatttttcg
    30421 tttcttctcc actagctaca gcgatgattt tttgaacaaa cgattctaag attacttcca
    30481 ttggttgttc taataagacg cctgcattaa aatccatcca atgacctttg cgttcaaaaa
    30541 gagttgtatt cgtagatacc ttgaccgttg ggacatatga gccaaacgga gtccctcttc
    30601 ctgtggtaaa caagaccaac tggcaatcgg atgaagcaag agcagaggca gctacaagat
    30661 cattcccggg agcttgcaat aaactaagcc ctggttttct gattttttcg ccatattgta
    30721 gcacatcaac gacaggagag cttcctgatt tttgcgtgca gcctaaagac ttatcttcta
    30781 atgtagtgat cccacctgct ttattaccag gagatgggtt ttcgtaaact ggttccccgt
    30841 aagacaagaa atactgtttg aagtcattga tcaagtcaac gattgagtca aaaacttctt
    30901 cattttccgc tcgtgccatc aatacttgtt ctgcgccaaa catttctggc acttctgtta
    30961 agatcgtgct gcctccttga gcaatcaagt agtcagaaaa tgcaccaagt aaaggattag
    31021 cagttattcc cgatagacca tcagaaccgc cgcatttcaa accgacattc aacttagaga
    31081 gcgggactga tatgcgctgg tcattttccg ccgctgttaa aagctcttct aatagagcca
    31141 caccttgttc gatctcatcg taaacatctt gagccactag aaatttcact cgttcttcat
    31201 catattcacc taaaattttt ttgaattcag gaactgtatt gttctcacaa cctaatccaa
    31261 atactaaaac tcctccggca ttgggatgat ttactgcgtc cgctaaaatt tctcgagtat
    31321 tttgatgatc ttttccaagc tgcgaacagc cataaggatg ctttaaaatc gtaatatggt
    31381 caaactgtcc cagatctgga tgtttttcct tgaactgttt gacgatcagt tcagcaatgc
    31441 cattcacaca cccaacggta ggtactaaaa acaaatcatt acgaatccct acttttccat
    31501 tttttcgcaa gtaaccttga aatgttcggt tctcaaaaag gtaatgactt ggatggacat
    31561 ctttttcata tcgataatcc aattcaccag aaagattggt ttttacatta tgagtgtgca
    31621 gccattgacc ggcagttacg tctgtcgtca catgaccgat tggatagcca tatttgatca
    31681 cgttgtctcc agctttcaaa tcacccaatg caatcttatg accttgcggg atatcttcaa
    31741 gagtggtgat cgtttgctga tcaatcgtca gttgtgtttg ttttggaata ggggttaatg
    31801 cgacgaccac actgtctcgt cgatttaatt tcattgttgg ttgcagggtt tcaagcatgg
    31861 accatcgttc ctttcatttc ttgtaatact gaccggacac ctttttgatc gatctttgca
    31921 gctatttctt gaactgtatc tgtcacattc ggtatttgag acaaatcttc tccccaaagc
    31981 gcagcgtttt gcaataatcg aggaatagct gttccattat cagaccaagc atcagtaaat
    32041 tcgcttatcg tcttttcatc atcatgtggc tttacttgat cgcctcgata cataagaagt
    32101 agcgcagcta gtgaaacagt gattaacggt ggtacctgat tttgttcttc gatatacttc
    32161 ttgaacactg gaagcagacg ggtgctgaac tttgaaacac tatttaatga aatcgcttct
    32221 aatttgtgat gggcaaaagg attcaagaaa cgttctttga tttggtcagc ataaatggct
    32281 aattcatctt taggtaaaga aagcatagga atcaattcta gattgaataa ctgatcaaca
    32341 tatgcggaga ataaaggatc tttcattact tcttctacag tttccaattc cgccaacaaa
    32401 gcaagaggga ccatcgctgt atgcggtcca ttaagcaaat gcaccttacg ttcacgataa
    32461 ggagtcatat catctgtgat gatgacattc aagccagcct gtttgaatgg tagttgattc
    32521 tccaattctt taggcccttc gatcacccaa agcatgaatg gttctgcttt gaccatcaat
    32581 tgatcttcat agttatgccg ttgattgaaa acacctgcat cttcgcgagg ataacctggg
    32641 acgatgcgat caactaaact acaacagaaa acattttctt tttctaacca ctcaacaaaa
    32701 gcaggttcta aatcccattt ttctgcgtag cgtaaaatga tttcttttag acgttcgccg
    32761 tttctgtcaa tcagttcgca aggaatgatg gtaaagccag ctttgtttaa tttgaaacgt
    32821 cggtataaaa aggctgtcag tttgcccggg aagctctgtg ggggagtatc tgttaaatta
    32881 tcagtctcca cgaattggat tcctgcctct gttgtattgg agacgatata ggccaagtcg
    32941 tcattttctg ctagagatag ataagcatcc cattcatcat aaggattgat catttgttct
    33001 actacggtga taatctcgga agtatcaacg atttcgccat tgctgagtcc ttgtaaaatg
    33061 accgtatata aattatcttg ctggttcatc atatttccaa gaccttgttc taaaggttga
    33121 atgattgcaa cattaccttt aaacaattgt tggttattca gctgctgaat ctgccaatca
    33181 ataaatccac gcataaaatt gccttcacca aactgaagaa cttttacagg tgcttcgttt
    33241 tttttcacta attcttttgt cagttttttc atataaactt cctttctgtg ttatgcacac
    33301 gttaacattt ttatataaaa ttacttgtag tttacaagta attgatcgac tcacgaagga
    33361 tcaacaaggt ggagagctca attttagatt gttcgatcgt cctgccttcg atcaaatcta
    33421 gtaaaagctt tgttccttct tgacacattt ctttcaaagg cttacgtacg gtggtaagcg
    33481 gcggattgag ataggagacg actggagaat catcgaaccc gatcagagaa atgttctggg
    33541 gaacacggag gccagctaaa tgtacagctc tcaatgcacc aatcgctatg tcgtcgttgc
    33601 cgcaaaaaac cagagtaggc aggtcagaaa gtgataatag gtattccatg ttttggaggc
    33661 cgctttctaa gctgtaatct cctgttaaaa aataggctgg attgacaggg atgctcgctt
    33721 tttttagact tttcaatacc cctttttccc gttcttttgt cgaacgaaaa tttttctttc
    33781 cgccaatata agcgatcttc gtatgtccgc atccaatacc gtaatctact gcctgcataa
    33841 caccggccgc atcatcggca gtgacgttga taaccgctgg atcattgatg tgacgattga
    33901 caacaacgaa aggaaattct ttttctttga gatagtcgat gaaaggctga tcatcatcac
    33961 tttgactcat aacgatcagt ccatcgtagc gttgcaggtt gatttctgtt aaatcaacaa
    34021 catcgatact ttcaacagat aaactgtaat ttgtgtctaa tactgaatgg atgcctgcaa
    34081 tggaatctgc taggaaattt gatgaagttc ctttatgcaa acttgagaaa aatagtccga
    34141 ttagatattt tttttgattc actaaacctt ttgcactata gtttggcgaa taattcattt
    34201 ccttagctag ttgtcgtatt ttttgttttg tttcattttt tattaaactg ctgttattca
    34261 aagcacgaga aactgtcgta tgagaaacgc cagctgcttt agcgatatct ttgatcgtta
    34321 ccattccatc attttcctta ctttttcact ttttgttaac gcattcatca tattacgata
    34381 actacgataa gtcaatagat ctttgatatg aaaagattgc acacgttaaa aattacttgg
    34441 atttttattt ttaattatgc tattcttgaa agcggggaat gctcatttaa ttagatgagt
    34501 atcaatttta cagaaaaatg aaagcctttt attttaaaag gaggagagga agaaaatgaa
    34561 gaaaagtaca gtattttata aaatattttt accgatgttt ttattaggag ccgtacttgt
    34621 cataggattt agtttattca tttaccagaa tacctatgaa tcgatcgaat cgagttattt
    34681 aacagacaaa aagaatttgc tcaggcaagt taaaactaac gttgaatgga aaattagaac
    34741 gattgaatat tcgttttcta cgtatggttc gaccaaaaat ttctcggaca tattcaagca
    34801 tccgctgaca tatacagatt actccactta ttctgaaatc aggaaagaac taaattttat
    34861 agaaacgatt gttatggatg ataataatta tgatttagtc agtttggctg gtaaatgggg
    34921 agtaatcaat ggatcgttga gtcaactgac cgaagaggaa gttgaaaagt atcaacagaa
    34981 gtacataaat aacaaaaata atttattttg gcaaaagaaa aacaaaggaa tcgaaatggt
    35041 gatttctttg ccaatgtttg aaaaagaaaa atatgcattg ggaattgcta cgatagagaa
    35101 acatacaata gaacaagttg ttggtgaaag tggagaagac atactttcta ttgaaaacga
    35161 agttggcaac ctttttacta ctcagacagt aaaaaataaa gagcaaccaa ctgtattaac
    35221 ggacgtggat ctagattaca atcagctgaa agtgataaga gaaaaagata aaacgtatat
    35281 agtactgaaa tcagattata ataattggat ttacagatta gaagttgact ctagtgcaat
    35341 tggcgcaatg atccgcaatc tgagaatcgg gctagttgca gtgtctttaa cactggtagg
    35401 actcattggt cttctttcct acttattttc ggacagattt gcccgtccga tttctcaaat
    35461 acaggaacgt ctgaatctta aatcaaaagg attcagtggg aaagaactag atttggtcgc
    35521 tcaatcagtc agtcggatca taggagaaaa agaagcgtta agtgcacact tagttaccca
    35581 aaagccacag ctggaaacgc tatttgtact tagtttattt agaaatcgag tagaaaggcg
    35641 agaactagat cagcggttgc agcaatttgg ttatagttct caaaatgact gctattatac
    35701 agctttggtt caaatcgatc tgttagaaga tagtcacggt ggggagcgtg atttgctact
    35761 tttagcgatc aacaacatga tcacggaaat tgtgccaaag gaaaatcgaa tgctgccgat
    35821 cgttttgaat gaagaaatgc aagccactat ttatcgaatg gataaagatg atgcagaagc
    35881 aagcaaaaaa gtcatggagt attgccgact cattcaaaaa cgtgtaaaag aatacttgaa
    35941 tttaactatc agtataggca tcagtaaccg gttcgaaacg ctgaatgaaa gcaagcaatc
    36001 ggttgatcgg gcaaaagaag ccctgtatta tcgtgtaaat actggtccaa gttcaattat
    36061 tttttatcag gaaatcgttc ctgctgctca tgaaaaaaca cttattagat atccgataga
    36121 agaacaaaat cgattgtttg aagccattcg ttctggaaat caggaagtga agaatttagt
    36181 acatgaattg attgatgcat tgtttgcaca aaataaaaat cctttgagtc gggaagtagt
    36241 ttcggtccgc ttgatcaatg aactcgttca attaggacag ttattgggag tagattcaaa
    36301 aaactttgat aacatgaaac aaatttatgt aaaagcactg aacaattatc atcccaaaga
    36361 actagaaaaa cttgttttag aacaattagt tctgccaatc acagaatgct ctcaagcaat
    36421 cactgatcat gaatttaaaa atttgtcgga gaatattatg catatcgtct acacagaata
    36481 tgatcgtgat ctgtctttag atgtgatcgc tgataggttg cattataacc ctaactattt
    36541 aagtaatgta tttaaaaagg aaacaggaga aaaatttggt gattttgttc aaaatcatcg
    36601 tttagaggta gcaaaaaaat ggttgaaaga gactaacctc tccgtaaaag agattgcaga
    36661 aagattgcaa tatcgaaatc cgcaaaattt tattcgtttc tttaaaaaga aggaatcgat
    36721 tacgccggga gaatatcgaa aaaaatattt gtgaatcaag aggctgtttt tcttgtggac
    36781 tgattcaagc aataaaagaa gttagaataa ccctagtatt tgtatttatg gatagttaaa
    36841 tttcaaggct aagcacgcaa aaagaactgt gacaaaagtc ttgtcacagt tcttttttac
    36901 gaataaatgg cgttcaagag acaggctatc gacaataagg cataattttg tgaaatatgg
    36961 gaagctattt ttcaaaatcg cttcttattg ctcctgtggg ttactcgtcg caatgtttgt
    37021 gagagttttt atcatcgctc aaggctgaac gtctctttca caacctctga tacacggtgt
    37081 tccaggagta gattcttcga aaaataagcc gaaatttcac aaaaatttaa gaagcaattt
    37141 tcgtaaatgc cttcttattt cttgaagctg accacttctg acacaactac cattttgcgt
    37201 tcagtacata atcacccttt gacagaacct acgagtgctc ctttgacaaa atatttctga
    37261 acaaaaggat aaacaatcag cataggcact gttgcaataa cgataacagc cattttaact
    37321 gtttgtgctg gtggaatcac atcaacggca ctactttctg cttgcatccc acttgataca
    37381 atgacgattt ggcgtaaaag aatctgtatc ggccatttag atgaatcgtt gatatacaaa
    37441 atagcagtca tataagtgtt ccagtaagta acagcataaa aaagcgagat agtagcaata
    37501 gacggtaacg ctaatggcag cataattttg aaaaatacac caaaatctgt gcaaccatcc
    37561 atttttgctg attcttccaa actatccggc aaggcttgga aaaagtttct cataatgatc
    37621 atattcattg cattaacagc cacaggcagg atcatggccc agtaagaatt gaccagccct
    37681 agagaacgaa caactaagaa cgttggaatc atccccccgc tgaacaacat agaaaataca
    37741 acaagaaagt tgacgaaacc tcgaccaaac aaatactttc tggaaagacc gtaggccatc
    37801 agtgaagttg cacacatact gacgatcgtt ccgacacctg taacaccaat cgaaacagca
    37861 agtgctctaa aaatggtcgg cgtagaaaga atgtagcgat acgcgtctaa agaaaaggta
    37921 gtaggaaaaa ggataaactt tttcgcgacg acttcttgtg tagaagcaaa agatgtagcg
    37981 atgatattta aaaaaggaac gatacagatc aaagctagca aaattagaaa cgtattgttg
    38041 aaaagtgtaa aacttctttc tcctatagag actttctttt tcatatagat aggctcctcc
    38101 tcaaaataaa ttaaatgttt ctgtttctat atgttcaatt tatcatcggt gaaagattac
    38161 gtatataatc atttcctatg ctaagtgtca gatagaaaaa aatagaaggt ttgctatgcg
    38221 tagtttattt gtgaaaaaaa acactttatc gttttgtata gcacaaaaat cattgctatg
    38281 taagcgtttt tacttacggt aaaaatgatt atatacgtaa ttcagatagt tatctatcat
    38341 gaagtcataa gcagcaaaca ttcacaaaac taaggagggg cttatggaaa gtgtaaagaa
    38401 acaagaacca tttcaactga cgctatcgaa aaaagaagaa aagctcagaa gacgaaaaaa
    38461 aataattgca ggaatcaaaa cgaataaatt tttatatgtg atgattctgc cgggagtcct
    38521 ttatttcttg atttttaggt atcttcctat ggggggactc gttattgctt ttcaagatta
    38581 tcaaccattt ttaggaataa tgggaagtcc ttgggtcggt ttcaaacatt tcattcgttt
    38641 gtttacagaa ccgacattct ttacattatt aagaaatacg ttgatcttgt tcggattaaa
    38701 tatcttcgtg ttttttccga tgccaatcat tttgtcttta ctattaaatg aagtaacgaa
    38761 caaacgattg aaaaacggga ttcaaacgat catttacatc cctcacttca tgtcttgggt
    38821 aatcattgtt tcgattactt atgtattttt gaatgttgac ggaggggtat tgaatgaatt
    38881 ccttgcaaca atgggatttg aaaaaatcag ttttctaaca tcccctgaat ggacacgcac
    38941 agtatatatc ggacaagtga tctggaaaga gttgggctgg tctacgatca tctacctttc
    39001 agcgataact gcagtcgata cacagttgta tgaagctgca gaaatggatg gagcaggacg
    39061 cttacgcaaa acatggcatg ttacattgcc ggcaattcgt ccagtaatca ttaccttgct
    39121 gattctaaaa atcggtcaga cattagactt agggttcgag catatgtact tgttattgaa
    39181 ttcattgaat cgaagcgttg cagaaatttt cgacacctat atttatacag ctggattgaa
    39241 gaatggacag ttaagtttca gtacagcaat gggattgttt aaaggggtcg taggattgtt
    39301 tatggtaatt ttagctaata agattgccaa aaagctagga gaagacggcg tttactaaga
    39361 tagaaagaga gggaaagata tgctgcagtc attagataaa ataaacggaa ggctaaatcg
    39421 tattttacag ttggtttatt tgaattttct gtggggcggt ttagtcatcg caggtttggg
    39481 agtttttact ttcggtcctg catcgtatgc gatggttgca gtgattagaa aatggctgcg
    39541 ctcaaaagaa acgtttcctt tgacgaaaac gtacttcaca tattttaaag aaaactatcg
    39601 tgaaaccttg ctgatgagct ggatatatgg cgccatcggg attgttttac ttgtggatct
    39661 ttctttcata cacaattggt atcttaggat cgctttgtat attgctgcgt tcatatatgt
    39721 gctctcagca ctctatattt tcccaatcat ggctcattat gattggaaag gatatttttt
    39781 caaaatgaaa atggcatttt tattcggatt ctcacatctt cattactcat tagtgttgat
    39841 attaagtatg gtagggattt atctgatgat catccaaaca atgccaggtg ttttaacctt
    39901 tatcggtggc agtctcttct tttccgcagt cacgtggaca gcaaattcta tattttgcca
    39961 tttggaatgg aagaactcat atacagaaaa aacagaagaa aacaaaaata ttattaaaac
    40021 agtgaaggag aaaatttatg aaaaaagcaa cgctcgtaaa attaggtctt agttttgcag
    40081 gaattctagc acttgccgca tgcggcagca gttcaagtaa cagttcttca gagacagaag
    40141 atggaaaagc taaaatcact tggttaaata ttttgcatca tgcttcaccg cctacagaca
    40201 cgattgtgaa cttgatcgaa gaaaaaaccg atacggaact gacattcaac tggattcctg
    40261 atgcttcaaa agaagaaaga ttaacaactg cattggcttc tgatgaacta ggagatatcg
    40321 ttacacttca aattgcgatg ttgaaaaact cttctgtccg caatgcgtta aaatcaggcg
    40381 tattctggga tgtatcagaa tatttaaaag actataaaaa tttaagtcaa atttctgaag
    40441 atagaatcga agctgcgagt atcgacggtg ccttgtacgg cgtaccgata caaaaagact
    40501 acgctcgtgc tggcttagtc attcgaaaag actggttgga taatctagga ctagatgttc
    40561 caactaccat ggatgagctt tatgaagtag ctaaacaatt tacagaaaac gatcctgatg
    40621 gaaatggtgt ggacgacaca gttggttttg gcgatcgtgc gcctagtgat attcgttata
    40681 ccagctttaa acttttcact tcttattttg gagcaccaaa tggctgggct gtagacgata
    40741 atggcaaatt cactccagaa tttgatacag aagagtatat gaaagctttg aatttttcaa
    40801 gagatctatt tgagaatggc tatttggcac aagactttgc agttacccaa aagactgatc
    40861 agcaagaaca atttgcacaa ggaagaactg gaatctacac tggaatggtg gatatcaaga
    40921 atctgaagac gatgggtcaa ggtattcaag atgatatgga attagtgcct gtaaacaaga
    40981 tctcagatgg tgaaggcggc ggttatcatg tttggtcaga aggaaatggt attggcggct
    41041 tgctagcatt tcccaaatct caagtaaaag acgaagcaca attgaagaaa ttacttcaat
    41101 ttgttgatga tcttttagaa gaagatgtgt ataaattgat gactggcggt atcgaaggaa
    41161 cgcattatga attgaatgat gatggttcta tcgaatttat cgatatggat ctttggcaac
    41221 aagaggtgca accgttatca agctctcgtc ctagtgaagt gacgttctct ttcaaagata
    41281 cagaccctga aaaagaattc tctaatcaat tgatcaaaga aaatgaagaa tttgctgtaa
    41341 tggatccttc tgtgccattg gattcaccga cgaacaatga agtaggatca gaaattgaaa
    41401 aaatcgtcat tgatgctacg gtgcaataca tcatgggaca gcttgacgaa gatggattca
    41461 aaaaagctgt agaagactgg aaagcacaag gaggcgatcg aatcacacaa gagtacgagg
    41521 aagcatacaa agcagcggca cagtaatata gacgattggg acaaatactt cttggtattt
    41581 gtcccaaatc ctattttaat ttttaaagga gagcaaataa tggaaaactg gtcaaagaaa
    41641 ttagcaaaaa gtatcatgga aagaacacct cgcttatatg aagaaaagtg gtataaagga
    41701 aaatggtctt atgactatgg cgtcgtactt aaagggttcc aattactttg ggaacgaacc
    41761 caagaaaaaa tttattttga tttcataaaa gacaatatcg actatttcgt gcaagaagat
    41821 ggaacgatcc gtggctacag cgttgaagaa tacaatatcg accatgtaaa tacaggaaag
    41881 cttttctttt tattgtacaa agaaacaggc gaagaaaaat ataaaaaagc agcagaactc
    41941 ctttcccagc agttggcaaa ccatccacgc acatccgaag gagctttttg gcataaagaa
    42001 atttatcctt atcaaatttg gttggatggc ttatatatgg gttctccttt ttatgctgaa
    42061 tttatccaga attttagtga agaaaaagat taccaagatg ttttgagaca gtttgaaatc
    42121 agttataaac atctattcga tccaaaaaca aatttgctga ttcatgcatg ggatgaaaag
    42181 aaggtccaac catgggctga tccaaaaaca ggtctatccc atcatttttg gagccgatcg
    42241 attggctggt atttgatggc tgcagcagat acctatgaat tacttcatga agaaacagat
    42301 tgttctttgc tgaaagaaat tctagaaaag acactggctg cattacttcc atatcaagaa
    42361 gaaaacagcg ggacatggta tcaagtaaca acagaaacaa aacggaaagg aaattatctg
    42421 gaagcttccg gcagtagtat gtttgtttac gcaatggcta aaggaatacg tttgggactg
    42481 ctgaacaaag aagaatggct tccacaagtg aagaaatcac atcaaggatt acttgatgaa
    42541 tttgttctgg aaacaaaaga aggctggctg aacttgaata aaaactgtca agtagcaggg
    42601 ttaggcggag cagatcagcg agatggttca tatgcttact acatcagtga accaattatt
    42661 tgtaatgatc aaaaaggcgt tggtgcattc ttgcaagcat tgattgaagt ggaggcatta
    42721 taaatatgta tgatattttg acatttggcg catcaattga tgaattgaat actgaagcaa
    42781 tacaacaagc cattgatgct gcagcaagtg acggaggcgg gaccgtagtt gttccagcag
    42841 gtgaatttct aacaggagct ttatttttaa aaagcaatgt cgaacttcat ttaagcgcag
    42901 gagcggttct gaaattctct gacgatccaa aagattatcc tgttgttcat tcacgttggg
    42961 aaggagtcca tcgaaaagtt tatgcttcct gtatctatgc tcaaaatgta gagaacatct
    43021 ctgtcactgg tttcggtaca ttagatggaa atggaaaaaa atggtggcat acttttagaa
    43081 atgaaccaga taatctcgct tatcctcgtc ctaaattgat gagtttccat aactgtcatc
    43141 gaattactgt gaaagatatt aaattgatcc agtcaccaag ttggacgatc aacccgattc
    43201 tttgcagtaa tgcaacgttt gataacttga cgatcttgaa cccagcggat tcaccaaata
    43261 cagacggaat tgatccagaa tcatgcaaaa atgtgcgtat tagtaattgc catattgacg
    43321 tgggagatga ctgtattgca atcaaggcag ggactgaaga tacgtacgaa aggatcgctt
    43381 gcgaaaatat cacgatcaca aattgcacga tggtccatgg acatggcggt gtcgtattag
    43441 gaagtgaaat gagtggtagt attcgtaata ttaccatttc aaattgtatc ttccaagaaa
    43501 ctgatcgagg aatacgattg aaatctagac gaggtcgagg cgggatcgtt gaagatattc
    43561 gagtatccaa tatagtcatg gataatgtga tgtgtccatt tatactgaat ctttattatt
    43621 tctgtggtcc gcgagggaaa gaaccgtatg tatgggaaaa aaaggcttat ccgatagatg
    43681 aacggacacc agcattcaga aggatccatt tttcgaatat cacggcaagg aatgtccatg
    43741 cttctgcagg ttttatttat ggattagcgg aacaattcat acaagaaatc acatttgaca
    43801 atatcgatgt ctcgatggcc caagatgcag ttcccggtgt tcctgctatg atgacaggaa
    43861 tagaggaaat gagcagacaa ggattttata ttggctttgc gggtaaggtt tcctttaacc
    43921 agatgtcgat tgaaaatcac gagggaccag catttctatt agaaaatact aaagaaatca
    43981 gtattgagaa attccaatca aatcaatcag aaaatatgga tgaactggtt gttcgtaaaa
    44041 actttgctta tgcagttgaa tgatttttta ggtgctccat ctagcagaca tacccctagt
    44101 ggaaaacgat tataccgaga aaaacaaaac ggttttatgc ttgaatacta tcaattgtac
    44161 ttaaatgaat tggaaacagt accagccctc tatgcttatc cagaaaaaga aggaccgttt
    44221 ccgccggtcc tttacctgca ttctcacggc ggagattttt cagtcgggaa aagtgaattg
    44281 atccatggtg ctccttactt agcttctcca tcttttgcag aagtactgac aggaatggga
    44341 tatgctgttt ggtcgattga tgcttgggga tttgaagaac gcggaggtat ttctgaaagc
    44401 gagttgttca aacaattttt attaacagga aaaacgttat ggggaatgcg tatttatgat
    44461 gtgatcagct tgatcgatta tttggagaca agggaagaca cagatactca acggctcgca
    44521 acgattggtc tttctatggg aggcttatta agctggtggg tgacagcatt ggattctcgt
    44581 gtcaaagttt gtattgatct agcggcacag gtggacatcg aaatactttt gctacatcgc
    44641 cgcctagatc atcatgggtt ttattactat gtgccaaatc tattgacagc ttatacaacg
    44701 ttagcaatcc aagaaaaaat cgctccgcgc cctcgtctta gcttagttgg aaaaaacgat
    44761 acgatgtgct tggacgaagg cgtactgaag ctgagaaaag ggctggacaa aaagtatcaa
    44821 tcaatgggta gtccagaaaa cttttcttct ttttctttaa caggcggtca tatggaaaca
    44881 caagaaatga gaaatatttg gaaacaattc ataagggagc atttatgagg atgaaagaca
    44941 aactgataat tggaaagaat gctatagata ccccgacgga ttttgaaact atccaagaag
    45001 gaatcgatta tttggcaggc ctgccggata gccatccaaa ggtcttgata gtaaaagaag
    45061 gaagatatga tgaatatata gaaagcagat tatccaattt taaacttgtg ggagcaggaa
    45121 cagtagttat ctccggtaat cgttttgcaa aacaggagta tcttgacggt agccagttag
    45181 ggactttccg aagtgctact tttttcttag aaggatcgaa tgtcgtatta gaaaatcttc
    45241 acatcgaaaa tacagcaggt ccaggagaaa aggtaggaca ggctgttgct ttattcaatt
    45301 caggagatcg cgtatcttta aaaaactgtc gactatctgg ttatcaggat accttatgca
    45361 cgggtccgtt gcctgatttg caaaaagatg gtactccttt ttgtacaccg gcttctgcca
    45421 atcctgagta ttgtcgtcag acctatgaat attgctggat tgaaggaacg gtggatttca
    45481 tatttggtgg ggcagatgcc gtttttcatc agtgtcatat ccatagctta gccagtaaaa
    45541 atcctggata catcacagca gcatctacgc cgaaaaatca aaaaacaggg tatcgttttg
    45601 aaagctgtac cttgactgca gaacctgata ctagaaacgt ctatttagga agaccgtggc
    45661 gtccgtatgc aaatgtctta tttgaagcgt gtaagatagg tgggcatgtt cacccaactg
    45721 gttgggataa ttgggatgac aagaaaaatg aacagactgt caaattctct gaacgtaata
    45781 acaattatga aacatcgatc aaaagacctg aatggataca tttagaaaag cagggggttc
    45841 agaaatgaaa agaaaatggg aactattgct aggaatggtc ggtggcagtc tatctttgat
    45901 tttctttggc gggttagcag ttactttaag taatatgtct gcgtcagagt ttaaaaaaag
    45961 ttatcagtcg cttgctgtag atcattcaac tttgtcttta gaaaatacat ttggattatt
    46021 acaggacatg actggtctgt ttgccgttgt gttgtttatt tcacttgctt ttttagcagt
    46081 agcattgttt ttgactgcta aaggaaaata cttaacaact gccactggtt tgtattttat
    46141 aacaggattc atattgttga ttggcactca gtttattgcg tttccttttg cattttttta
    46201 tttcgcagct ggggcattca gtttatatcg agtaagaatg agaaaaggag cctgaatcat
    46261 gcagactatc aaagtgaaaa atcctatttt aagaggattc aatccagatc ctaatatatt
    46321 aagagtgaaa gagcggtatt acattattgt ctcctctttt gaatggcttc caggaatccg
    46381 agtatatgag tcgaaaaatt tagtcaactg gtcacataca acggatattt tgaccactca
    46441 agtatctttg caaggaaatc ctacaaatgg aagtatatgg gcgccacaaa tcagttatgc
    46501 tgatggattg ttttatcttg tttacacaga tgtcaaaagt gcaggccgtc cgtttaaaga
    46561 tgtccacaat tatttgatca ctgcatctga gatcacaggt ccttggtccg aaccaattta
    46621 tttaaatagt aacggctttg atccttcttt gttccatgat cttgaaacgg gaaaaaaatg
    46681 gcttgtcaat gaactatggg attatcgtct ggacacaccg aataaatcca gcggtatcgt
    46741 attacaagaa tatgatgcag agaagaagaa gcttgatggt cccatctata aaatatttgc
    46801 aggtacagaa ttagcgaaaa cggaggcacc acatttgtat tttcgtaacg ggtattatta
    46861 tttgatgacg gcagaaggcg gtacaggaag aggacattca gttacagttt gccgagcaaa
    46921 gaatattact ggtccttacg agcttgatcc tggttttcct atgctgacag ctagtgacaa
    46981 acctgcttct acattgcaat gtacaggaca tgggagctta gtacaaaccc cgtcaggaag
    47041 atggtacatg acatatcttt gtaccagacc attaaacggt gcggctattt tgggaagaga
    47101 aaccgctatt caagaagtgt attggacgtc cgacgattgg ttaagactta aagatagcca
    47161 gacaactccg atggaagaaa taatgattga gacaaatgaa gaggtacaac aagaaaaaga
    47221 tcatcagttt atggatacat ttgaaggaga gctaaagaag aactggaatg ccagacgcat
    47281 tttgccaaat gctgattggt gtgatacaaa atgtagacca ggttttttac gtctgatttc
    47341 cggtgaatct cttcaatcta ccttcgaaca tcatttgtta gctattcggc aaacggattt
    47401 tacttttgat gcggagacag catttgaata catgcctaat aattttaatc agatggctgg
    47461 actggtactg tacttaaatg aatccaatta tatttattgt tatgttactt ggaatgagga
    47521 caagggaaaa tgcctacgta tgatagaatc agaaaacgga gtagcacaaa tagaagattg
    47581 gacgattcca ctcacagaaa agaagacttt tttgaaagtc actgtaaaga aagagacagc
    47641 acagtttcat ttgaaagacg tatcaactga agaatggaag aaagtaggtc cgcaaaaaaa
    47701 catgtgtata ctctcaggag ggtttactgg aaattttatc gggatagcag tgcatgatct
    47761 aaataaaaaa agaggaagtt atgctgattt tgagtttttc aagtatgatg gaaaagatca
    47821 tctgtaattt aattctttaa taaatgaggt tcactaactt attttcacta gttagtgaat
    47881 ttttttgttt gtatatattg ttatgtaaaa aaagttatca atcttatgca tcaatgtttt
    47941 tagattattt tttgtttttt atctgtcaag ataatgcaat aaaaagccaa cattgtgaat
    48001 ataaaaacaa gaaaaaaaga gttataattt tgaaaacgtt tactagaaga ggtaggagga
    48061 attcaaatgc tttcacaaaa tgctgttaca gcaaatacaa taaaaaaaga aaaaaaggaa
    48121 tttgggattt ccgataagat aggctacatg ttcggtgatc taggtaactg ttttatttta
    48181 ggtttagtca atagtttctt gatgatttat tatacgaatg ttcttggcgt ttcaggggca
    48241 atcgtaggta cgttattctt gatttcgaga attttggatg cgtttgctga tgtgatggtt
    48301 ggaagattat gtgatgtctc agagttaaca gaaactggcc gatttattcc ttggatcaga
    48361 aaaatgaagt atccattttg cttagtgact gtcattttgt ttttgccatt cgtccaacat
    48421 cttccaatga cggtgaagat tgcttatatt tttgtttcat acattgttta tgggattttt
    48481 ctctcaacaa tcaatatccc gtatggagca atggcagcag caattagctc aaatcctaat
    48541 gaccgcacct cgttgtccac gttccgaagc attggttctg cgattggcgg atcaactaca
    48601 ggattcttca ttccgatttt aatgtatacc cataacaaga gtggacaaca agtcgtttca
    48661 gggaatcatt tcttttggat ttcaatcgga tgtgctgcaa tagcatttgt cgcatatatg
    48721 ttaacttgta aattgactac tgaaagagta agagttgtca aaaacgaaaa agtatctgct
    48781 tctcagttgg taaaaggttt gttagctaat cgagcactga ttattttagt cattgttgac
    48841 attttcattg tgatcaatca gattttagct gggacgaata tgacttattt attcaatgat
    48901 tacttccata ataaacaagc aatgtctgtt gctcttttat ttacttacgg aacagtagta
    48961 gttttagcac catttgctac aaagctgaca aaattatttg gtaaaaagga agcaagcgta
    49021 tttgcgttac ttgcttcttc atgtatgtac ttgctgatgt atttccttca tataacaaat
    49081 gcatgggtat atctgattct tttatttatc gctactttag gatctggttt attcaatttg
    49141 atggtctggg cattcattac tgacgtgatt gattatcatc aatataagac aggcttaaga
    49201 gaagatggaa cagtatatgg agtaaattct tttgcgagaa aagtaggaca agcatgtgca
    49261 ggagcggtag gcggatttat gctggcactc attggttacc aatcgtctac aacaggtgga
    49321 gccatccaat cagttttagt acaagaaagg atttatacga tggcaaactt gttaccagca
    49381 ttctgcttat ttgtatccgc aatcattttg ctagtaggtt acccactgaa tcgttcaaaa
    49441 actgtattta ttggtgaaga attgaaaaaa atcaatgaaa gatagggaga aacgtattgc
    49501 ggacaaaaaa agggattatc aatgctttga actttaaaac aaacgacact caacaagatg
    49561 atacaataat actgcaaaaa gcaatcgatc aaggtgcgaa agaacactta cctgtattta
    49621 ttccaaaagg catttattta gtcggagccc tttttttaaa agataagagt catcttatct
    49681 ttgaagaagg tgcagtcctc aaggggagaa cagatatcga agcatttcca gaaatcgata
    49741 cacgagtagc cggcgtagaa atgaaatggc cagcagcgat tttgaatgtt ctctcggcga
    49801 aagatattct tatagaagga aaaggaataa tcgacggtca aggagaccat tggtgggagc
    49861 tgtattgggg aaaagatcaa aaaagcggga caagagctga atacgatcaa aagggacttc
    49921 gttggattgc ggattatgct attaaacgtc cacgcgcttg tttactgtat catgcagaac
    49981 atgttgttgt tcgtgatttg atttttcaga aatcaggttt ttggaacttg caaatcactt
    50041 attctaacga tgtattagta gaaaaagtaa tcatccgcaa caatgatgga ccaagtacag
    50101 atgggattga tattgattct tcaacaaatg tgcgtgtcta tgaatgtgat ttggcctgtg
    50161 gtgatgattg tattgctatt aagtctggca gagatggcaa tggtgcaaga gtcaatcgaa
    50221 aatcatcaag aatcgaggtt gctagatgta aaatccgttc aggatacggt gtaactattg
    50281 gtagcgaagt tagtgctgga gttagtgacg tatatattca cgatatcgat ttttttcagt
    50341 cagactgtgg tattcgaatg aaatcttcaa aagaaagagg gggagtgatc gaaaatatca
    50401 gggtagaaaa tctgaatatg attgatgtac aatttccttt ttcttggata atggactggc
    50461 ataatgaata caatagaaaa aatagcaatg accttgctca aatgccagaa agttggcaag
    50521 cagtggctga agaaatacct gaatcaaagc aaatgacgaa agtcaaagac atacacatca
    50581 aaaacgtaca ggcatgcctt tctccaggtt actcacttcc ttcccgagct tttgatttag
    50641 tagcatttcc tgaaaagcct atagaagatg tctgcttttc tcattgccaa ataactgcaa
    50701 aagaattcgg gcgaatcgaa gcagtacaaa acttatgttt caaaagctgt gatttatcta
    50761 tagaaactgg caacacagct gcaaacaata cttttgacaa tcgttgaaat tataataagg
    50821 tcaagcttaa ataatatttt agcttgacct ttattttttt taatgatata ttaaacaacg
    50881 ttgtcgcgct tcattgaaac attttatgaa aattccaaaa aagagttgac acatgttttt
    50941 agacgtgata agataactaa gtcgttaaaa agaaccactt gcgaagcttg attctatcga
    51001 aaaaaacttc aaaaaagttc ttgacaaata acaaacgata agttatgata aatgagttgc
    51061 tgaaagattt cgaaaaacga aatcgaaaaa aacttttaaa aaagagttga caatcgtttg
    51121 tctctctgat atgatataaa agttgctacg gcagccaaaa atgaatcggg aaacactctc
    51181 gatgaagtag acctttgaaa actgaacaaa gtaagacaaa ccaaatgtgt agggcgtctt
    51241 gattcaattc aagacaa
//
LOCUS       DAZAID010000010        51072 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000010 DAZAID010000000
VERSION     DAZAID010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.010"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..508
                     /locus_tag="ACG9BE_000500"
     CDS             137..508
                     /locus_tag="ACG9BE_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFN3031281.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(782..1474)
                     /locus_tag="ACG9BE_000501"
     CDS             complement(782..1474)
                     /locus_tag="ACG9BE_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3031282.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1477..2262)
                     /locus_tag="ACG9BE_000502"
     CDS             complement(1477..2262)
                     /locus_tag="ACG9BE_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN3031283.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2499..3899
                     /gene="lacG"
                     /locus_tag="ACG9BE_000503"
     CDS             2499..3899
                     /gene="lacG"
                     /locus_tag="ACG9BE_000503"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFN3031284.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3924..4241
                     /locus_tag="ACG9BE_000504"
     CDS             3924..4241
                     /locus_tag="ACG9BE_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN3031285.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4254..5927
                     /locus_tag="ACG9BE_000505"
     CDS             4254..5927
                     /locus_tag="ACG9BE_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFN3031286.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5941..7572
                     /locus_tag="ACG9BE_000506"
     CDS             5941..7572
                     /locus_tag="ACG9BE_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFN3031287.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8063..9628)
                     /gene="guaA"
                     /locus_tag="ACG9BE_000507"
     CDS             complement(8063..9628)
                     /gene="guaA"
                     /locus_tag="ACG9BE_000507"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFN3031288.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9687..10610)
                     /gene="coaA"
                     /locus_tag="ACG9BE_000508"
     CDS             complement(9687..10610)
                     /gene="coaA"
                     /locus_tag="ACG9BE_000508"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFN3031289.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10806..11411
                     /locus_tag="ACG9BE_000509"
     CDS             10806..11411
                     /locus_tag="ACG9BE_000509"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3031290.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11719..12735
                     /locus_tag="ACG9BE_000510"
     CDS             11719..12735
                     /locus_tag="ACG9BE_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3031291.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12876..14177
                     /locus_tag="ACG9BE_000511"
     CDS             12876..14177
                     /locus_tag="ACG9BE_000511"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFN3031292.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14223..14885
                     /gene="deoC"
                     /locus_tag="ACG9BE_000512"
     CDS             14223..14885
                     /gene="deoC"
                     /locus_tag="ACG9BE_000512"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFN3031293.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14906..15304
                     /locus_tag="ACG9BE_000513"
     CDS             14906..15304
                     /locus_tag="ACG9BE_000513"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFN3031294.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15370..16452
                     /locus_tag="ACG9BE_000514"
     CDS             15370..16452
                     /locus_tag="ACG9BE_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3031295.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16664..18226
                     /locus_tag="ACG9BE_000515"
     CDS             16664..18226
                     /locus_tag="ACG9BE_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3031296.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18219..19352
                     /locus_tag="ACG9BE_000516"
     CDS             18219..19352
                     /locus_tag="ACG9BE_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3031297.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19352..20308
                     /locus_tag="ACG9BE_000517"
     CDS             19352..20308
                     /locus_tag="ACG9BE_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3031298.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20407..21573
                     /gene="deoB"
                     /locus_tag="ACG9BE_000518"
     CDS             20407..21573
                     /gene="deoB"
                     /locus_tag="ACG9BE_000518"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFN3031299.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21597..22415
                     /locus_tag="ACG9BE_000519"
     CDS             21597..22415
                     /locus_tag="ACG9BE_000519"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFN3031300.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22432..23139
                     /gene="deoD"
                     /locus_tag="ACG9BE_000520"
     CDS             22432..23139
                     /gene="deoD"
                     /locus_tag="ACG9BE_000520"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFN3031301.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23437..24123)
                     /gene="gpmA"
                     /locus_tag="ACG9BE_000521"
     CDS             complement(23437..24123)
                     /gene="gpmA"
                     /locus_tag="ACG9BE_000521"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFN3031302.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24295..24876)
                     /locus_tag="ACG9BE_000522"
     CDS             complement(24295..24876)
                     /locus_tag="ACG9BE_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3031303.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25009..25692)
                     /gene="rpiA"
                     /locus_tag="ACG9BE_000523"
     CDS             complement(25009..25692)
                     /gene="rpiA"
                     /locus_tag="ACG9BE_000523"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFN3031304.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26011..26424
                     /gene="rpsL"
                     /locus_tag="ACG9BE_000524"
     CDS             26011..26424
                     /gene="rpsL"
                     /locus_tag="ACG9BE_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFN3031305.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26508..26978
                     /gene="rpsG"
                     /locus_tag="ACG9BE_000525"
     CDS             26508..26978
                     /gene="rpsG"
                     /locus_tag="ACG9BE_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFN3031306.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27072..29159
                     /gene="fusA"
                     /locus_tag="ACG9BE_000526"
     CDS             27072..29159
                     /gene="fusA"
                     /locus_tag="ACG9BE_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFN3031307.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29331..30518
                     /gene="tuf"
                     /locus_tag="ACG9BE_000527"
     CDS             29331..30518
                     /gene="tuf"
                     /locus_tag="ACG9BE_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFN3031308.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30925..31233
                     /gene="rpsJ"
                     /locus_tag="ACG9BE_000528"
     CDS             30925..31233
                     /gene="rpsJ"
                     /locus_tag="ACG9BE_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFN3031309.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31264..31893
                     /gene="rplC"
                     /locus_tag="ACG9BE_000529"
     CDS             31264..31893
                     /gene="rplC"
                     /locus_tag="ACG9BE_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFN3031310.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31921..32544
                     /gene="rplD"
                     /locus_tag="ACG9BE_000530"
     CDS             31921..32544
                     /gene="rplD"
                     /locus_tag="ACG9BE_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFN3031311.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32544..32834
                     /gene="rplW"
                     /locus_tag="ACG9BE_000531"
     CDS             32544..32834
                     /gene="rplW"
                     /locus_tag="ACG9BE_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFN3031312.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32873..33706
                     /gene="rplB"
                     /locus_tag="ACG9BE_000532"
     CDS             32873..33706
                     /gene="rplB"
                     /locus_tag="ACG9BE_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFN3031313.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33749..34027
                     /gene="rpsS"
                     /locus_tag="ACG9BE_000533"
     CDS             33749..34027
                     /gene="rpsS"
                     /locus_tag="ACG9BE_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFN3031314.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34050..34397
                     /gene="rplV"
                     /locus_tag="ACG9BE_000534"
     CDS             34050..34397
                     /gene="rplV"
                     /locus_tag="ACG9BE_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFN3031315.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34411..35067
                     /gene="rpsC"
                     /locus_tag="ACG9BE_000535"
     CDS             34411..35067
                     /gene="rpsC"
                     /locus_tag="ACG9BE_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFN3031316.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35070..35504
                     /gene="rplP"
                     /locus_tag="ACG9BE_000536"
     CDS             35070..35504
                     /gene="rplP"
                     /locus_tag="ACG9BE_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFN3031317.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35494..35682
                     /gene="rpmC"
                     /locus_tag="ACG9BE_000537"
     CDS             35494..35682
                     /gene="rpmC"
                     /locus_tag="ACG9BE_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFN3031318.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35707..35973
                     /gene="rpsQ"
                     /locus_tag="ACG9BE_000538"
     CDS             35707..35973
                     /gene="rpsQ"
                     /locus_tag="ACG9BE_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFN3031319.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36031..36399
                     /gene="rplN"
                     /locus_tag="ACG9BE_000539"
     CDS             36031..36399
                     /gene="rplN"
                     /locus_tag="ACG9BE_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFN3031320.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36436..36744
                     /gene="rplX"
                     /locus_tag="ACG9BE_000540"
     CDS             36436..36744
                     /gene="rplX"
                     /locus_tag="ACG9BE_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFN3031321.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36772..37311
                     /gene="rplE"
                     /locus_tag="ACG9BE_000541"
     CDS             36772..37311
                     /gene="rplE"
                     /locus_tag="ACG9BE_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFN3031322.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37330..37515
                     /locus_tag="ACG9BE_000542"
     CDS             37330..37515
                     /locus_tag="ACG9BE_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFN3031323.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37551..37949
                     /gene="rpsH"
                     /locus_tag="ACG9BE_000543"
     CDS             37551..37949
                     /gene="rpsH"
                     /locus_tag="ACG9BE_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFN3031324.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37981..38517
                     /gene="rplF"
                     /locus_tag="ACG9BE_000544"
     CDS             37981..38517
                     /gene="rplF"
                     /locus_tag="ACG9BE_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFN3031325.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38678..39034
                     /gene="rplR"
                     /locus_tag="ACG9BE_000545"
     CDS             38678..39034
                     /gene="rplR"
                     /locus_tag="ACG9BE_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFN3031326.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39055..39555
                     /gene="rpsE"
                     /locus_tag="ACG9BE_000546"
     CDS             39055..39555
                     /gene="rpsE"
                     /locus_tag="ACG9BE_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFN3031327.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39570..39749
                     /gene="rpmD"
                     /locus_tag="ACG9BE_000547"
     CDS             39570..39749
                     /gene="rpmD"
                     /locus_tag="ACG9BE_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFN3031328.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39793..40233
                     /gene="rplO"
                     /locus_tag="ACG9BE_000548"
     CDS             39793..40233
                     /gene="rplO"
                     /locus_tag="ACG9BE_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFN3031329.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40233..41528
                     /gene="secY"
                     /locus_tag="ACG9BE_000549"
     CDS             40233..41528
                     /gene="secY"
                     /locus_tag="ACG9BE_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFN3031330.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41590..42237
                     /locus_tag="ACG9BE_000550"
     CDS             41590..42237
                     /locus_tag="ACG9BE_000550"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFN3031331.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42428..42646
                     /gene="infA"
                     /locus_tag="ACG9BE_000551"
     CDS             42428..42646
                     /gene="infA"
                     /locus_tag="ACG9BE_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFN3031332.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42679..42795
                     /gene="rpmJ"
                     /locus_tag="ACG9BE_000552"
     CDS             42679..42795
                     /gene="rpmJ"
                     /locus_tag="ACG9BE_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFN3031333.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42813..43178
                     /gene="rpsM"
                     /locus_tag="ACG9BE_000553"
     CDS             42813..43178
                     /gene="rpsM"
                     /locus_tag="ACG9BE_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFN3031334.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43206..43595
                     /gene="rpsK"
                     /locus_tag="ACG9BE_000554"
     CDS             43206..43595
                     /gene="rpsK"
                     /locus_tag="ACG9BE_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFN3031335.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43675..44613
                     /locus_tag="ACG9BE_000555"
     CDS             43675..44613
                     /locus_tag="ACG9BE_000555"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFN3031336.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44641..45021
                     /gene="rplQ"
                     /locus_tag="ACG9BE_000556"
     CDS             44641..45021
                     /gene="rplQ"
                     /locus_tag="ACG9BE_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFN3031337.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45293..46132
                     /locus_tag="ACG9BE_000557"
     CDS             45293..46132
                     /locus_tag="ACG9BE_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN3031338.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46108..46977
                     /locus_tag="ACG9BE_000558"
     CDS             46108..46977
                     /locus_tag="ACG9BE_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN3031339.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46967..47764
                     /locus_tag="ACG9BE_000559"
     CDS             46967..47764
                     /locus_tag="ACG9BE_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFN3031340.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            47967..48713
                     /gene="truA"
                     /locus_tag="ACG9BE_000560"
     CDS             47967..48713
                     /gene="truA"
                     /locus_tag="ACG9BE_000560"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFN3031341.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            49377..49571
                     /locus_tag="ACG9BE_000561"
                     /pseudo
     CDS             49377..49571
                     /locus_tag="ACG9BE_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(49759..50535)
                     /locus_tag="ACG9BE_000562"
     CDS             complement(49759..50535)
                     /locus_tag="ACG9BE_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN3031342.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            50658..51065
                     /locus_tag="ACG9BE_000563"
     CDS             50658..51065
                     /locus_tag="ACG9BE_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFN3031343.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT        16210 a         9283 c        11078 g        14501 t
ORIGIN      
        1 cactcagaga acgggttggt ttttaataga tttaataagt actgataatg aaatttctcc
       61 tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt actttgcgga
      121 gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag tcatcaatca
      181 atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg ccattatttc
      241 ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg ataaagacaa
      301 aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag atcttaagtt
      361 taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga aagatacgga
      421 aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa ctcagcttgg
      481 tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa aagtatatca
      541 aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa acactatttt
      601 taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa
      661 gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg tttcactggc
      721 agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt agttgaatcg
      781 tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta aaatctttga
      841 tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta ccgaatggat
      901 caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct gagtaagcat
      961 cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct ttaagaaaaa
     1021 tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa
     1081 accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg acagtagccg
     1141 tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct tcaagccctt
     1201 ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag ctttcttttt
     1261 cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa
     1321 tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt
     1381 ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag tccaaaaaca
     1441 ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag tggtattttc
     1501 ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa cctcccgcga
     1561 gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc
     1621 atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat tatttagagc
     1681 gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc
     1741 atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg
     1801 tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa tttctggtcc
     1861 atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc gaattttctt
     1921 gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga aaatcgtttc
     1981 tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt gaatctgcag
     2041 agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac tttgagcccc
     2101 tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc tgcgaacggt
     2161 catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc cttttttatt
     2221 gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca cctctaattt
     2281 aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt
     2341 tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt ttttatgatt
     2401 atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa
     2461 agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta ccagaagatt
     2521 ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag gaaggtggca
     2581 aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt cccgatccgg
     2641 caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa
     2701 atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct gggaaaccaa
     2761 atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa
     2821 cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac catggagatt
     2881 ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt tttcatgaat
     2941 tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca accaatcaat
     3001 atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc gtcgcctgtc
     3061 ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa aacgaacttc
     3121 cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca gacgcgccgg
     3181 aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta ttagatgcta
     3241 cctatttagg ctattattca actgaaacct tgactgctct tgatgagata tgtgaagcaa
     3301 atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa gcatccacgc
     3361 gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct tacgatggag
     3421 aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg
     3481 gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac tggctgattt
     3541 atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca cattacaaca
     3601 aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat ggcattatta
     3661 tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc aaggcgataa
     3721 cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt ttctcatgga
     3781 caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc cagaagcgtt
     3841 acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg attatttaat
     3901 aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt ggttttgaga
     3961 tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa gaagtaagac
     4021 aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg
     4081 cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag atggagatgg
     4141 gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg agagatctga
     4201 ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac gctatggatg
     4261 gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca cgtaatagtt
     4321 atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta ttttcaagca
     4381 ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct gaatctgtct
     4441 ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta ctcgtttcag
     4501 caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa
     4561 tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta agcgtctctc
     4621 cgattgaagg tggattttca acggaatata tggggacaaa gggactactg gcttcctttg
     4681 tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat attaccatta
     4741 aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt tttcctttct
     4801 ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc ttccaaatct
     4861 catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct gacgggtact
     4921 taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt gttcatggac
     4981 cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa accaatctgc
     5041 aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta gggaattttg
     5101 taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc ctactctttg
     5161 ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta agtttcgctg
     5221 tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt tttattccat
     5281 ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca
     5341 tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt ttggttctag
     5401 gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc gttgacttcc
     5461 tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa gaagcacaaa
     5521 aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag gaagtacctg
     5581 tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg gtactttgtg
     5641 ctggcgcagg gacaagtggg atgttagcta atgctttaaa aaaaggtgca tctgaatcga
     5701 aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg ctgcaggatt
     5761 ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc aaagaagaca
     5821 ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc ggattaacga
     5881 gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg aggagccata
     5941 atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt aggggctttg
     6001 ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg agcagatatt
     6061 tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga tatttcaaaa
     6121 gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg tttgtggcag
     6181 gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct ggaaacgacg
     6241 attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga ttttcactac
     6301 agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca aggtctgacc
     6361 tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc ggaaatgaag
     6421 cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag cggaatgctc
     6481 tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt ggctgctttt
     6541 ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc agtaatgctc
     6601 cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga aatcacgagt
     6661 cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca cggttcaatg
     6721 aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga agtgatcgta
     6781 gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa tgcttttggt
     6841 gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc agagtatttg
     6901 ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg cttcttctat
     6961 tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg tatgaattat
     7021 ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt tgattttgct
     7081 ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt
     7141 aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg gcaaacctgg
     7201 tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt ttttggggac
     7261 tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca aaatgttagt
     7321 ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg agagtttgat
     7381 actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc ggttgttaaa
     7441 acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa
     7501 attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt tgtttcattg
     7561 gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt cagccacatc
     7621 gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac
     7681 gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac actttgtatt
     7741 atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc
     7801 agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg aatatgattt
     7861 gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg agaacttctt
     7921 tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta gggaaccatc
     7981 ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta tagggattcc
     8041 ccctccatat tatgttggct ctttattccc actccactgt tgctggtggt ttagaagtga
     8101 tgtcatacac gatacggttg acgtgggcta cttcgtttac gatacgtaca gagatttttt
     8161 gcaagacttc ccatgggata cgggcaaagt cagcggtcat tccgtcgatt gaagttacag
     8221 cacggatacc gactgtgtag tcgtaagtac gtccgtcacc cataacaccg acagagcgga
     8281 tccctggaag aacagtgaaa tattgccaga tatcacggtc aagtccagca gctgcgattt
     8341 cttcacgcaa gatagcatca gattcgcgta cgatttctag cttgtcttca gtgatttcgc
     8401 caagaacacg aataccaaga ccaggaccag ggaatggctg acgccaaacg attgaatcag
     8461 gcatgcctaa ctgagtccct aattcacgga cttcgtcttt gaataatgtg tttaatggtt
     8521 cgatcaattc aaattgcata tcttctggca gtccacctac attatggtga gatttgatcg
     8581 tctgtgctgt ttctgtacca gattcgataa cgtcagtata caacgttcct tgtgctagga
     8641 aagagacgcc ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca tatacgaatt
     8701 cattcccaat gattttacgt ttttgttctg ggtcagatac acctttcaac ttgtctaaga
     8761 agcgatctct tgcattgact ttgatgatgt tcaagccgaa ttttccgcct agactttcca
     8821 ttacttgttc cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata gatgtcaatt
     8881 ggtcaccgat cgctttttgt aaaagaaccc caacgacact agaatcaacg ccacctgaaa
     8941 gaccaagcaa gacttttttg tcaccaactt gttcacggat tttttctact tgcatatcga
     9001 taaagttatc cattgtccag ttaccttcac agtgacaaac atcaaaggca aagtggcgca
     9061 gcaattcgat tccgtattcg gaatgacgta cttctgcatg gaattggata ccataaaaat
     9121 tacgtgttgt gtcttggata gaagcaatcg gacaatcttt gcttgttccg actgtttcaa
     9181 agccttcagg aactttagtt accaagtcac catggctcat ccatactgtt tgttgttttg
     9241 gtgtttcttt aaacaagaca gcttgatcgc ttgttacttc taacaaggct ttcccatatt
     9301 cgcggttttt agcaggctct acggaaccgc caagtttgta cgtgatcaat tgcataccat
     9361 agcaaatccc taatacagga atacctaaat tgaagatctc aggatcgatc ccaaatgcat
     9421 tctcatcata tacactgttt ggtccgccag aaagaatgat tccctttgga ttcatcgctt
     9481 ttacttcttc tgcagtaatg cggtggctca ataattcgga aaagactcca agatcacgaa
     9541 tacgtcttgt gatcaattgg ttatactgac taccgtagtc taatacgata atcttttcaa
     9601 cgttcgtcat atcggcaacg tttgtcaaat ttattacccc tatcccttta aattttatct
     9661 caaaatagtc aaactatttt aaaatgctaa tattttcgca tgaaaatcga atcgatgata
     9721 tgattttcag ttttatggag aattatatcg gctcttcctc gtgtaggcaa gatatattcc
     9781 tgtaaatttt tcaaattcac atttttccat acatcttttg ccattttaaa ggcttcttca
     9841 cgatctccga tagcaaaacg ataataataa ttctctggat tttggaaagc cgtatccagt
     9901 aacgcaccaa aacgatccaa ataccatttt tcgatcagca cgggatcggc atcgacatag
     9961 acagaaaaat caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa ttgaagcgta
    10021 ttgatcccct caacgatcaa gatatcaggc tgctggatca attcgtactt gtctggaatg
    10081 atatcataca catcatgtga gtaaacaggt acttttatct cttcctgacc agattttaca
    10141 cggttcaaga aatcgatcag catctccata tcataacttt caggaaatcc tttacggtcc
    10201 atgattccac gctcttttaa tatacgattt ggataaagaa atccgtcagt cgtgatcagc
    10261 tgaacgtttc ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct ggctgttgta
    10321 ctttttccaa cggctacact tccggcgatc ccgataataa aaggcggtac ttctacgtat
    10381 ttatgaagaa ataaaccttt actcaagaac aaagattcaa attctttcat ataaagataa
    10441 atcagatgag caagaggtac atagatatct cgaacatcct gcatggaaat gcggtcattc
    10501 aaacttttga tattatctag ttcatcttgt gtcaaaggtg cttgcccatt acgataaaat
    10561 tctcgccatt cttctcttgc cacacggtag tagttcattg gatcattcat attagcctcc
    10621 tgcgttctcc catcaatgga actattttaa cacaaaataa acgcaaaccc caccacttaa
    10681 gagagattgc cgatcttttt gtataatctt tataatagaa agaaacggag tcttattctt
    10741 gacttgcgca cggatacata aaaggataga ctgttgtcat gaaagaaatg agaaggatgg
    10801 aagacatgac aaatcattac tattctgaac gcccagatac ggcgcacgat tttgaacaat
    10861 gggcttttga gttaaaaggc agaacctttc aatttgtaac agattctggt gtattttcac
    10921 gtgaaacagt ggattatggt tcacgtgtcc tgattgatgc tttcgattgg gaagagttac
    10981 ctgatgaagg aaagatctta gatgtgggat gcgggtatgg accgattggg ttagctttgg
    11041 catttgcgac acagcggttt gttgaaatgg tcgacattaa tgaacgagcg gtaagtctag
    11101 cgcaaggaaa cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa tcgaatatct
    11161 atgaagcagt tcatgaagaa acctatgcag ctatcgtaag caatccgccg atccgtgctg
    11221 gaaaaaaagt tgtacatgaa attttgacag gcgcatatcc tcggttgaag aagggcggta
    11281 cactgacaat tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag aaaatggaag
    11341 aaacatttgg taatgcagaa atcgtcacaa aagataaagg atattatatc atcaaaagtg
    11401 tgaaagaata actagctgaa aaatagtgga gcgacgaaag agtggaactg ggcgtgcatg
    11461 tacgggtacg tatcagttct ttttttgttg agaagttttt aaaaacaaag aactattttt
    11521 tcttcaaaaa gaaatagtag gaacagagtc gttgtttttt acgataaaat aaataagata
    11581 cagaacttgt atagaaggct gccaagtcgg tagataaagg aggtttttat caaaaaacat
    11641 actttccttt tttctctatt cttttcttgg gggttttggt acactgatag ggaatttaga
    11701 tagaagggag ccaataggat gaagctgacc atcagagata tcgcagaaat ggcaggcgta
    11761 tcagtaacaa ctgtctcaca gattttgaat aacaaaggaa gcagatttag tgataagaca
    11821 agaaaaaaag tgttgtcgat cgtgaatgaa catcattata agcccgatta tttcgcttca
    11881 aatgtgatca atcgacattc aaagaccatc ggaatgatcg tccctgatgt cactgatttc
    11941 ttcttctcca aactgatcga aggtgtagag agctatttga atcctttggg ctatgtgatc
    12001 atgttatgca attctcgtca tagccaagaa aatgagatca agtatttaca agagctttcc
    12061 caccgctcag tggacggaat cttgctggcc acaccaaata ttttacctga gaaatatgca
    12121 ttagacagcg gtttttatca aaaaatgcca atcattttaa tcgatcgcgg attgaatcgt
    12181 cgggataacg gacggcttat cgtaaaggaa tatgagggcg cctatcaagc tgtttcgctg
    12241 tttattgaaa atggccatac gaagatcggc atgctgaagg aaaccactgg atactatcaa
    12301 ttagaagaac gtttcaacgg atatcgtcat gcgctgaaag atcatggcct gccttttaat
    12361 gggaaatttg ttgaacatgg agagttgact gtccaaggtg gatatactgc atcaaaaaaa
    12421 ttactcaaac ataaagatat cacggctatt ttttgcggga atgatgccat ggcgatcgga
    12481 tgctatcagg cgatcgatga attgggcaaa aaaatcccag aagatatctc agtcatagga
    12541 ttcgatggat taaagctgtc agaatacatg attccgcaat tgacgacagt gcagcagcca
    12601 agttttgata ttggatttta tgctgcacgt ttcctgatcg atacgatcga atttcctcaa
    12661 agaaaagttc cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag cgtaaaagta
    12721 ttgacaaagg gctaactcgg cggtatattg cttatgtaag catttacaaa ggctagttag
    12781 tgcttttttt atttttagta aaccagttta ctaaagtggt ttacctttcc taaaaataat
    12841 gtataatacc gaatgaagac aaggaggatc aggcaatgcg aatggtggat ctgattgaaa
    12901 aaaagaggga tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc acgaactata
    12961 cgaatggaaa gattccagat tatcaagtca gtgcattatt gatggcaatt ttttatcaag
    13021 acatgacaaa tgaagaaatc acgaacttaa cattggcgat tgctaattct ggtgatgtca
    13081 tcgatttgag ttctcttgaa ggaataaagg tagataagca ttcgacaggt ggcgttggtg
    13141 acacgaccac attgatttta gcccccctag tagctagtgt cggagtgaca gtggctaaaa
    13201 tgtctggacg cggtctaggt tacacaggtg gtacactaga caagctagaa gcaattcccg
    13261 gatttcagat cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa agtaaagtag
    13321 cggtaattgg tcaatcagga aacttggcac cggcggacaa aaaattgtat gccttgcgtg
    13381 acgtcacagc gaccgttgat tctcttccgc tgattgccag ttcgattatg agtaaaaaaa
    13441 tcgcggcagg agcagatgca atcgttttgg atgtgaccac tggagacggt gctttcatga
    13501 agaacatcga agatgcaaga cgtctggcaa agaccatgac gagcattggg aaactggcta
    13561 accgagaaac agtggcggtt atctcagata tgtcagaacc tcttggcgaa gctatcggaa
    13621 atagtctaga agttgtcgag gcaatcgaaa cattacaagg aaatggtcca gaagatctag
    13681 tagaaatgtg ctacgcctta ggaagccaaa tggttgttct tgctggcaaa gctaagacaa
    13741 ccgatgaagc aagaacgttg cttcaagaag ctctggaatc aggtaaagcg ttagcaaaat
    13801 tcaaagaaat gatccaaaat caagggggag acccagcgat cgtggaacat ccagaacgaa
    13861 ttttgactgc acgctacaca atggagcttc ctgctaaaca aagcggagtt gtgtcaaaaa
    13921 tcgtggcaaa tgaattagga attgctgcaa tgatgctagg tgctgggcgt aaaaccaaag
    13981 aagaagatat tgatcacgct gttggattga aactgcataa aaaaattggt gacactgtga
    14041 caaaaggtga gtcactattg acgatctata gtaacgacga agaaatcact tctgtcattg
    14101 aattattata taaaaatatt gaaattggtg aatcggcaat gaagccaacg ttgattcatg
    14161 acatcatcac agaataaagg aaaaaagcgg actttaccgt tttaaagaaa aggggaaaag
    14221 aaatggaact aaaccgaatg atcgaccata ctattttgaa agcagacgca tctaaagaag
    14281 ctgtgatgca aattatcgaa gaagcaaaaa aataccattt ttattctgtc tgcatcaatc
    14341 cagcatgggt tgcgctagcg aaagaacaat tacaaggtga gccagtagcc gtatgtacag
    14401 taatcgggtt tcctctagga gcaaacacac ccgagacaaa agcatttgaa acaacagatg
    14461 ccatcaataa cggtgcagat gaagtcgata tggtcatcaa tatcggtgct ttgaaatcaa
    14521 aggatgatca acaagtccaa aaagatatcg aagcagttgt tgaagcagca aaagatcgtg
    14581 cgcttgtcaa agttattatt gaaacatcgt tattagatag agaagaaatc atccgagctt
    14641 gcgaaatagc aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt ttctcaacag
    14701 gcggagcaaa ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca gaaatgggtg
    14761 tcaaagcttc cggcggtatc cataacgaag aagaagcaat ggcaatgatc gaagctggtg
    14821 ctacaagaat cggaacaagc gcaggagtag caatcgtatc tggttcaact ggtgaaggct
    14881 attaattttt tcaaaggaga agctcatggc agaactcaaa caagaatgga tcaacacagc
    14941 tattgaagcg ttagataaag cctatgttcc ttattcccat tttccggtag gcgcttgtct
    15001 cgtaacggaa tcaggcaaga tttatcaagg catcaacatt gagaatgcat catttggatt
    15061 aacgaattgt gccgagcgca cagctttttt caaagcagta tctgaaggag aaaaaacatt
    15121 tacgcatcta gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg gtgcatgtcg
    15181 acaagtaatg gcagaattct gttcccctga tatgccggtt acgctagtag gagacaatgg
    15241 tgtgacgaag gcaacgaccg ttcgtgcact gctgccttat gcatttactg aaaaagatct
    15301 ataactcatg ttcattcagt cagctgactg ttgaaaaata aaacaattat tttaggaggc
    15361 tttacagaaa tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact agcagcaggg
    15421 ttatttttag gtgcatgcgg caacaacggc tctactgatt catccggtgg caaaagctca
    15481 tcagacacaa caactgcagc attgatcaca gacacaggtg gtgtagacga ccgttcattc
    15541 aaccagtcag cttgggaagg actagaaaaa tgggggaaag atcaaggact ttcacgagga
    15601 aatgatggct tccaatattt ccaatcttca aatgaatcag attacatccc aaatattgac
    15661 caagcattga atgctggatt caaaacgatt tttggtattg gttataaact aaaaccagcg
    15721 atccaagaac aagcaacaaa caatacaggt acgaactttg ttattatcga tgatgtcatc
    15781 gatggattag ataacgttgt atctgcaact ttcaaagaca acgaagcttc ttatcttgca
    15841 ggtgttgcag cagcttatac gactgaaaca aacgtagtcg gctttatcgg cggtgtaaaa
    15901 ggtgaagtaa tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc aggtgcgaaa
    15961 gaattaggaa aagaaatcaa agtattgaat caatatgctg gtgacttctc tgctccagat
    16021 aaaggacgtt caattgctca aggtatgtat gcacaaaacg cagatatcat cttccatgct
    16081 tcaggcggta caggtaacgg tgtattccaa gaagcaaaat cattgaacga atcaggcgac
    16141 aaaaaagttt gggttatcgg tgttgaccgc gaccaatcag acgaaggcga atacacatta
    16201 aacggcgaaa agaaaaactt cacattgaca tctacactga aagcagttgg aacagtagta
    16261 gaagatttgg cacaaaaatc agcggacggt aaattccctg gcggcgaaca cactgtttat
    16321 ggattgaaag aagacggtgt tggtctaaca gaaggacaac tttctgacga agcgaaaaaa
    16381 gcagttgatg aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc agaaactcct
    16441 gaagaaaact aaaaccattt ctatagcctt gttagacaca tgtttgacaa ggctatggtt
    16501 tgattagaac aagaattgtt catttatcca agctgccttg tgacagtttg gataagggaa
    16561 caactccctg aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt gaatcttagt
    16621 caatcgttgc cctagataga aatcgttagg atgtgaaaaa aaggtggttc aagaaaatat
    16681 tgtcatagaa atgcagaata ttaccaaaga atttggaaca ttcaaggcaa atgacaacat
    16741 caatttacaa gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg gtgctggcaa
    16801 atctacattg atgaacgtgc tttccggatt attagagccg acatcaggga aaattttgat
    16861 gcgtgggaaa gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag ggattgggat
    16921 ggtccatcag cactttatgc ttgttgatgc ctttactgta acagaaaaca tcgtgttggg
    16981 aagcgagcct agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag agatccaaaa
    17041 agtttctgaa cagtatggat tgtcagtcaa cccagatgct tacgttcgtg atatttcagt
    17101 tggaatggaa caacgggtag aaattttaaa aacactttac cgaggagcag atgtactgat
    17161 ttttgatgag ccgacagctg tattgacccc tcaagaaatt gatgaattaa tcgtgatcat
    17221 gaaggaatta gtcaaagaag gcaagtcgat cattttgatt acgcataagt tagatgaaat
    17281 caaagcagta gctgaccgct gtacagttat ccgccgtgga aaaggaatcg gtacagtcaa
    17341 cgttaaagac gtaacctcac aacaattagc cgatatgatg gttggaagaa cggtttcatt
    17401 caaaacgatg aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg aaaatctagt
    17461 ggtgaaagaa aatcgtggat tagaagccgt gaaaaacttg aacttagagg ttcgtgctgg
    17521 cgaagtactt ggtatcgctg gaatcgatgg aaacgggcag tcggagttga tccaagcttt
    17581 gactggtttg cgaaaggcag aaagcggaca tatcaagcta aaaggggaag acatcactaa
    17641 taaaaaaccc cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag accgtcataa
    17701 atacgggtta gtcctagata tgacattatc tgaaaatatc gcactacaaa cctatcatca
    17761 aaaaccttac agtaaaaacg gcatgctgaa ttattcagta atgaatgaac atgccagaga
    17821 attgatcgaa gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa aagctttatc
    17881 aggcggaaat cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc ctgatctgtt
    17941 gattgttgca aatccaactc gtggactgga tgtaggtgcg atcgaattta ttcataaacg
    18001 tctgatcgaa caaagggaca aatacaaagc agtgttactg atcagtttcg aattagaaga
    18061 aattttaaat gtttcggatc gtattgctgt tatccatgaa ggagaaattg tcggtatcgt
    18121 tgatccgaaa gaaacatctg aaaatgaatt aggtctattg atggcgggct ataccttaga
    18181 agaagcacga aaagagttag aaaagggggc tggcgcacat gaatgatcgc agcgaaaaat
    18241 tccgtaattt attagtgcct gtactttcag ttgttttagg attgatctta ggcgcgatat
    18301 tgatggctgc ttttggtttt cagcccatcc aaggttattc ttctatgcta gatgcagccc
    18361 ttggttcgca aagagggata ggtgaaacgt taaggcaagc aacgccgttg atttttacag
    18421 cattaggttt ttctgtagct aactcagctg ggtttttcaa tattgggctt tctggacaag
    18481 cactttgcgg gtggatcgtc agtgtctggg tggcacttgc gtttccagat ctgccaaaag
    18541 ttgttttgct tccgttatgc gtgatcttag gtgccttagc tggtgcgatg gcagcggctg
    18601 ttccaggttt acttcgagca ttttttggaa caagtgaagt gatcgtaaca atcatgatga
    18661 actatatctt attatacgta agcacatttg ctttacatga agtgatgcct gaaagctatc
    18721 gctcaagttt ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg ttaccatggt
    18781 taacacaagc ttttggcggc tctcgcgtaa actcaggatt atttttagca atcatcgcgc
    18841 ttattttcgt ttggctgctg atgaagaaaa cgactttagg atttgaaatc cgttctgttg
    18901 gattgaatcc ttttgcttct gagtatgcag gaatgagtag taaacgtacg atcgttttgt
    18961 cgatggtcat ttctggcgct ttggcagggc ttggcggtgt ggttgaagga ttaggaacgt
    19021 atcaaaactt ctttgttcaa acgacttcgc tatctattgg atttgacgga atggctgttt
    19081 ccttgcttgg ttctggatca gcaatcggta ttcttttatc agcgatcttg ttcagtattt
    19141 tgaagatcgg tggacttggc atgcagacag gagcaggtgt tccgtttgag atcgtcaatg
    19201 tatcgattgc tttgattatc ttctttgtcg ggatcaactt cctgattcgt ttcgtcatgg
    19261 ctaaagtctt ccaagggaaa aaacaagaag agatcgttgc gacaatagaa tcgaaaccaa
    19321 cgaatcaaaa ccatgaaggg ggcgaactat aatgtcgact atcgaattaa ttgccttgat
    19381 ccttacttct acactggttt attcaacacc gctaattcta acgtcactag gcggaacttt
    19441 ttctgaacga agcgggatcg taaacgtagg gctagaggga atcatggtca tgggtgcttt
    19501 tagttccatc gttttcaact tgacgatgag tccatcactt ggttctatga caccatggat
    19561 cgctatttta gtgggtggcg tcgtaggtat cctattttct ctgattcacg cagtagcaac
    19621 agtcagtttg cgtgctgacc atattatcag cgggacagta attaatctga tggcacctgc
    19681 tttaggtgtt ttcttgatca aagcatggta tggtaaaggt caaactgata acatcacgca
    19741 gaatctgggg tatttttctt tcccaggatt agcagatatt ccggtcattg gaccaatttt
    19801 ctttaaaaat acattcttac ctgcttattg tgctattttg attgctgtcc tagcttgggt
    19861 gatcatcttc aagacgaaat tcggcttgcg cctccgttca gttggtgaaa atcctcaagc
    19921 tgccgacaca ttaggtatca atgtatatgc tatgcgttat tcaggcgtac tgatttctgg
    19981 cttgttaggc ggtatgggcg gtgctgtttt tgctcagtcg atttccggta acttttcagc
    20041 tggtacgatc gttggacaag ggttcatctc catggctgct atgatctttg gtaagtggaa
    20101 tcctttaggg gcaatgggtg cctcattgtt ctttggtttt gctcaaagct tgagtatcat
    20161 tggcgcacaa ttaccagtta tttcttcgat tccgccagta ttgctgcaaa ttgctccata
    20221 tttgctgaca atcattgtgc tggttgtttt cttagggaaa gcttctggtc caaaagcaaa
    20281 cgggaagaac tatatcaaat caaaataatc ttttagcgaa taaaaagggt gggttacggg
    20341 aagtgttttt cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa gaaagaagga
    20401 ataatcatgt ttaaacgcgt acatctgatc gtcatggatt cagtaggaat tggagaagca
    20461 ccagatgccg ataagttcgg tgatgttggt tctgatacat taggacacat tgcgaaagaa
    20521 gcaggattga acgttccaca tttagagcag cttggtttag gaacgatccg tcctcttgaa
    20581 ggtgtggaat cagtgaaaga tcatcaaggc tatgcgacga aattagaaga agtttctgta
    20641 gggaaagata cgatgacagg ccactgggaa atcatgggat tgaatatcaa aactccattt
    20701 cgtgtgtttc ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga attttctggc
    20761 cgtaaagtcg tatgtaacaa accttatagc ggaacagctg ttattgacga ttacggtgaa
    20821 caccaaatga aaacaggcga tttaattgta tatacttctg ccgatcctgt ccttcagatc
    20881 gcggcacatg aagaaatcat tcctttagaa gaattatatc ggatttgcca attcgttcgt
    20941 gatataacaa aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc gtacgtgggt
    21001 gaaccaggag acttcacacg aacaagcaat cgccatgatt atgcgttgaa tccattcgga
    21061 cacacagtcc ttgattcttt gaaagaagca ggaaaggacg tgattgctgt tggaaagatc
    21121 aacgatattt tcaacggaca aggaatcact gaatctgtcc gcacgaaaag caatatggat
    21181 ggcgttgacc agctgctgaa agttatgaaa aacgattttt caggaatcag ctttaccaat
    21241 ttagtagatt ttgatgcatt atacggtcat cgtcgggatg tagtcggcta tgcgcatgcg
    21301 attgaagaat ttgacttacg aattccagaa ttgcttgaag cgatggaaga agacgacttg
    21361 ctaatgatta cagccgatca cggaaacgat ccgacgttta ctggtaccga tcatacgcga
    21421 gaatatgtac cgttattggt ttacagtaaa aaaatgaaag aacaaggggc attgcctcaa
    21481 ggatattatt cagatatttc agcaacgatt gctgagaact ttagtgtttc ttcgactgaa
    21541 aatggcgaaa gcttcttgaa attattaaaa taatcttttt aaataggaga aagcaaatga
    21601 ccaagttaag cgagatgtta caagaaacga cccagtttat ccttgataaa ggagtaaaag
    21661 aaattgattt tggtttgatt ctaggttcag gattaggtga attagcagat gaaatcgaag
    21721 atgctgtaat gatttcttat gatgagatcc cgttctttcc tgtttcgaca gtagttgggc
    21781 atgcaggaaa gctagtttat gggtctctgg caggcaagaa agtgttagcc atgcaaggga
    21841 gattccacta ctatgaaggt cattccatgc aaacagtgac ctatccagta cgtgtgatgg
    21901 ccggactagg ggcccattca gtcattgtga ctaatgcatg tggtggtgtc aacgaatcat
    21961 ttgtaccagg agatttgatg ctgattaccg atcatatcaa ttttactggt gaaaatcctt
    22021 tgatcggtcc gaatgaagat gcaatgggcc ctagatttcc agatatgagt caagcttata
    22081 cgcctgacta tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg gatcttaaag
    22141 aaggcgtata tatgggctat tctggaccaa cttatgaaac gcctgcggag atccgaatgt
    22201 ctcggacaat aggagccgat gcagtaggga tgtcgactgt tccagaagtc atcgtagcag
    22261 ctcacagcgg attaaaagtg ttaggaatct cttgtattac caacttagca gcaggtatgc
    22321 aagcgaatct gagccatgaa gaagttgtag aaacaactca gagagtcaaa caatctttta
    22381 aagcattggt taaagaaaca ttgacattat tgtaatagga ggaacaataa aatgagtgtg
    22441 catattgaag cgaaagaagg cgagatcgcc gataagattt tacttccggg agatcctttg
    22501 cgagctaaat atattgcgga gacttttttg gaagatccag tttgttacaa tcaggtacgc
    22561 ggtatgttag gctttaccgg caaatataaa ggagaacgta tttctgttca aggaacagga
    22621 atgggaatgc catcagccac catctatgcc catgaattga tccagtcgta tggtgtaaag
    22681 aaattgattc gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt tcgtgattta
    22741 gtactagcac aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt ccaagccttt
    22801 cattttcctc caatcagtga tttcaatcta ttactgaaag catatgagat agctaaagaa
    22861 aagggctata cggttcatgt aggaaatgtt ctttcggaag attctttcta caaagatgat
    22921 ttgacagaga cgttccaatt agctgaactt ggcgttttag gagtggaaat ggaagcagcg
    22981 gctctttatt acttaggcgc gaaataccat gtgcagacat taagtttgat gaccgttagc
    23041 gaccatttga tcaccggtga agaaacaaca gctgcagaac gtcaatctac gttcaatgaa
    23101 atgatcgaag taggattgga aacagcgata gcagaataag aaggaaatga aaaagctgcc
    23161 ctgcattaag taataagaac atccaagcaa aaatagctcc tcatgttttt gacttggctg
    23221 gacttattat tgcaaatgta agcttttgaa caccgtttac caagaggttg tgaatctgcc
    23281 gtttaactct gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt tgatgaatat
    23341 tgtgacttgt gaccgagaag ttggcaggag aaaaccgttt atacgaaaaa agggactgtg
    23401 acaaatcgtg tcacagtccc ttttttatag tgttttttat aggtagtatt ttttagcaac
    23461 tgttaagtca tcatttaatt cataaacaag tggttgacct gttggaattt caagatccat
    23521 gatgtcttcg tcagagatac cttcgatatg tttagctagg gcgcgtaatg agttaccatg
    23581 tgcagctact aaaacagttt tgttgtctag taatgctgga gcaatttcat cttgccagaa
    23641 tggcaaagcg cgttctaatg tcactttcaa gttttctcca ccaggaatat cgcgtttgtc
    23701 taacattgcg taacgacggt catttgctgc agaaccttcg tcagtttctt ccattaatgg
    23761 aggaagagta tcgtaagaac gacgccagat gtgcacttgt tcgtcgccgt atttttcagc
    23821 tgtttctttt ttgttcaaac cttgtaattt accatagtga cgttcgttta agcgccatga
    23881 tttgatttgt ggtacccaaa gttgatctga atattctaag atcaagttac atgttttgat
    23941 tgcacgagtt aaaacagaag tgtaagcaac atcaaattcg attccagctt ctttgatttt
    24001 gcgtccgcct tcttttgctt cttcgattcc ttctggtgct agatttacat cagcccaacc
    24061 agtaaattgg ttcaatgcat tccattcact aagaccgtga cgagaaaaaa ctaattttgg
    24121 catgtttagc tctcctttta agataggttt atatttctga aattttcagt tctgttttat
    24181 tgtacaattt tttggaagag atttccatcg aaaaattaga taagattcta tgagaaaaca
    24241 agtagagaat ataaaaaaac gaacagtgcc cacgcaactg ttcgttttta ggtattagat
    24301 gcataattgc tcgatttctt tttcaggtaa attgatatat ttataacatg ttccaactga
    24361 aacaccgcat tttgaagaaa taaattggat cgtttctttt ttttcataat agagacgtcg
    24421 gatttttttg atcgtttttg catcgatttt tggtcttccg ccgatttttc cttttttacg
    24481 agcttcatct aatccaacta atgtacgttc tttgatcaat gcgcactcca tgtttgctag
    24541 gttttccatt aattggaaat agatttttcc catttcgcgc gtatcgattc cttcatcaag
    24601 actaatcaag tgcagcccgc gatctttgaa taattgcgtg agttctgtta attgccgcgt
    24661 agatttgcct aatcgatgaa gttggcaaat gactagtgca tcgccttgtt gcatgtcagc
    24721 gatcactgac tccaaatgaa cagcagtaga atcattttgt tccacttcat atgtctcatg
    24781 gaaaatttct tgacaaccaa agtcagataa ttgatcaacc tgagttgtta aatcaacgtc
    24841 atttatggtt gtgcgtgcat aaccaataat tttcatggtg attcctttct agcagtgatt
    24901 ttctaactgg atataacagt aaaaatgtag cataaagtac gtcaaaaaga tagttttttc
    24961 gcaaattttc cgctgtcttt ttttattgga tgatcaagca gtttttcttt atttacgacg
    25021 gattgcgtca agaatctcaa cgcctttttc tgagccgaca actactttag aaaccatgtt
    25081 taggaaaagc ccatgttcta ctaccccaac aagttcatct aaataggtag ctaagctttc
    25141 tggctgttcg atgacttcta gatgcagatc aatgatgtaa tgaccaccat ctgtggttaa
    25201 gacttctccg ttttcgtctg tacgcaagac gggttggaat cttttttctt caaaaatatg
    25261 aagtagctgt tgggagccat aagggatcac ttcaactggg agtgggaact tcccaagttt
    25321 atcgactaat ttagaagaat caacgatcca aatcgtttct tttgagtacg tagcgactat
    25381 tttctcgaaa agcaaagcag cgccgccgcc tttgatccct tgaaaatcag cactgatttc
    25441 atcagcaccg tctattgtca aatcgactac ggggacttca tcaatactct ttaacggtat
    25501 tcctaatttt tccgcttgtt cttttgtcgc atttgaagta gtaactcctg tgatacgtaa
    25561 accttcttca cgcatacggc gaccaatctc ttctaccata taataggcgg ttgaaccggt
    25621 cccgagacct acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc cagctaattg
    25681 ttttaaattc atctttgcgt ccaccttttt ttagtttgag catcgtgctt tcctcactat
    25741 tttagcgtga tttacggtaa aaagatacca ttctttttta ttccaataaa gctttttcaa
    25801 aaaaaattgc atctcttgtt gacatgtttt ttcattcgtg atattctaac aaaggtgctt
    25861 tatctacagg tttctgtgaa gaaacgtgct gactgtttat caaagcatat ttgataagga
    25921 tgcagacatt gcatttattt tttatcacaa aaaaagaacc acctggatgt gtggaactag
    25981 acgcaaatcg tgggaaggag gaaacaagga atgcctacaa ttaaccaatt agtacgtaaa
    26041 cctcgtaaat caaaggtgga aaaatctaat tcacctgctt tgaacaaagg atataacagc
    26101 tttaagaaaa ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc aactcgtgtg
    26161 ggtacaatga cacctaaaaa accgaactca gctttacgta aatatgcacg tgttcgtttg
    26221 tcaaacttaa ttgaagttac tgcttatatc ccagggattg gtcataactt acaagaacac
    26281 agtgttgtat tattacgcgg tggtcgtgta aaagacttac caggggtacg ttaccatatc
    26341 gttcgcggtg ctttagatac tgctggtgtt aacgaccgta aacaaagccg ttctaaatac
    26401 ggaactaaaa aacctaaagc ttaattttaa ttattaaaca aatgaaactt gcttctacta
    26461 tatagaagta attactatga acaatatttc ggaaggagga gttacggatg ccacgtaaag
    26521 gtcctgttgc aaaacgtgat gttttaccag atcctattta taactcaaaa ttagtaactc
    26581 gcttgatcaa ccgtgtaatg gttgacggaa aacgcggtat tgcagctaac attatctata
    26641 atgcctttga cttgatcaaa gaatctacag gtaacgatcc attagaagtg tttgaacaag
    26701 caatgaaaaa cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg ggttcaaact
    26761 accaagtacc agttgaagtt cgccctgaac gccgcgttac tctaggtctt cgttgggtag
    26821 taaactacgc tcgcttgcgc ggtgaacaca caatggaaga acgcttagca aaagaaatca
    26881 tggatgctgc aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact cataaaatgg
    26941 ctgatgccaa ccgtgcgttt gctcactatc gctggtaaga ttctctctgt ttggtcggct
    27001 tttataagtc gacctcagat agtgtcctta tttttagcaa ctagatagta aagagaggag
    27061 aaacaaggag aatgaccaga gaattttctt tacaaaacac tcgtaatatc ggtatcatgg
    27121 cccacatcga tgctggtaaa acgactgcta ctgaacgtat cttgtactat actggtaaaa
    27181 tccataaaat tggtgaaact catgaaggag cttcacaaat ggactggatg gaacaagaac
    27241 aagaacgtgg tattacgatc acttctgctg ctactacagc acaatggaag ggtcaccgaa
    27301 tcaacatcat cgacacacca ggacacgtag acttcacagt tgaagttgaa cgttcattgc
    27361 gtgtacttga tggtgctgta actgttcttg atgcacaatc aggtgttgaa cctcaaactg
    27421 aaacagtttg gcgtcaagca acaacttatg gtgtacctcg tatcgtattt gcaaacaaaa
    27481 tggacaagat cggtgctgac ttcttatatt cagtatcaac tttacatgac cgtttgcaag
    27541 caaacgctca cccaatccaa ttaccaattg gtgcggaaga cgacttcaca ggaattatcg
    27601 accttgtaac aatgaaggct gaaatgtata caaacgatct tggaacagag atcgaagaaa
    27661 cagaaattcc tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa ttggttgaag
    27721 ctgtagctga aactgatgaa gaacttactt tgaaatactt agaaggtgaa gaaatcactg
    27781 aagctgagtt aaaagaaggt atccgccgcg caacagttaa cgtagaattc tacccagtac
    27841 tatgtggttc agcatttaag aacaaagggg ttcaattact attggacgct gttcttgatt
    27901 acttgccatc accgttagat atcccagcta tcaaagggat tgaccctaaa actgatgaag
    27961 aagtagaacg tcctgcagac gacagcgctc ctttctcagc tttagcattt aaagttatga
    28021 ctgacccatt cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt ttacaatcag
    28081 gttcatacgt acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt atcctacaga
    28141 tgcatgccaa ctctcgttca gaaatctctg aagtttacgc tggagatatc gctgctgctg
    28201 ttggtttgaa agacacaact actggggaca cactatgtga tgaaaagagt ctagttattc
    28261 ttgaatccat ggaattccca gaaccagtta tccaagtcgc tattgaacct aaatcaaaag
    28321 ctgaccaaga taaaatgggt gtggctttac aaaaactatc tgaagaagat cctactttcc
    28381 gtgctgaaac aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt gaacttcact
    28441 tagatatcat cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac gtaggtgcgc
    28501 ctcaagtatc ttaccgtgaa actttccgtg ctggcactca agccgaaggt aagtttgtac
    28561 gtcagtctgg tggtaaaggt caatacggtc acgtatggat tgaatttaca ccaaatgaag
    28621 aaggtgctgg tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct cgtgaataca
    28681 ttccagctgt tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta gctggctatc
    28741 cattagtaga tatcaaagct aaactttacg atggttcata ccatgatgtc gattcaaatg
    28801 aaactgcctt ccgtgttgct gcttctatgg cattacgtgc agcagctaag aaagctaatc
    28861 cagtaatcct tgaaccaatc atggctgtag aagttgttat ccctgaagat tatttaggtg
    28921 atgttatggg tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa gctcgcgctg
    28981 gcggccaaca agttgttcgt gcgatggttc cacttgctga aatgtttggt tatgctacaa
    29041 ctcttcgttc tgctactcaa ggtcgtggta catttacaat gacatttgac cactacgaag
    29101 atgtaccaaa atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa gctgaataat
    29161 ttcttgaaac cggatagtcc tgcaaagaga ttatccaaag agactatatt tttaatatag
    29221 tttcgtgtaa aataattatc gatgatatgg gcatgtggtg taccttgtgt acatcatata
    29281 cctatcagac aaataaaaaa tatattgtat ttttaggagg acattttaaa atggcaaaag
    29341 aaaaatttga ccgttctaaa ccacacgtta acattggtac tatcggacac gttgaccatg
    29401 gtaaaactac attaacagct gcaatcacaa ctgtactatc taagaaaaac ggcggccaag
    29461 ctatggctta cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga atcacaatct
    29521 caacagctca cgttgagtat gaaacagata cacgccacta cgctcacgtt gactgcccag
    29581 gacacgcgga ttacgttaaa aacatgatca ctggtgctgc tcaaatggac ggagctatct
    29641 tggtagtttc tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc ctattgtctc
    29701 gtcaagttgg tgttccttac atcgttgtat tcttgaacaa agtagacatg gttgatgacg
    29761 aagaattact agaattagtt gaaatggaag ttcgtgacct attaacagaa tacgaattcc
    29821 ctggtgacga tgttcctgta gttgctggat cagctttgaa agctctagaa ggcgacgctt
    29881 catacgaaga aaaaattctt gaattaatgg ctgcagttga cgaatacatc ccaactccag
    29941 aacgtgacaa cgacaaacca ttcatgatgc cagttgaaga cgtgttctca attactggac
    30001 gtggtactgt tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt gacgaagttg
    30061 aagttgttgg tattgctgaa gaaacttcaa aaacaacagt tactggtgtt gaaatgttcc
    30121 gtaaattgtt agactacgct gaagctggag acaacattgg tgctttacta cgtggtgttg
    30181 cacgtgaaga catccaacgt ggacaagttt tagctaaacc aggtacaatc acacctcata
    30241 caaaattctc tgcagaagta tacgtgttga caaaagaaga aggtggacgt catactccat
    30301 tcttcactaa ctaccgtcca caattctact tccgtacaac tgacgtaaca ggtgttgttg
    30361 aattaccaga aggaactgaa atggttatgc ctggcgacaa cgtaacaatg gaagttgaat
    30421 taatccaccc aatcgctatc gaaaacggta ctaaattctc aatccgtgaa ggcggacgta
    30481 cagttggtgc cggtgtcgtt acagaaatca aagcttaatt cacttaagtt ttaaagtaaa
    30541 gcaacattta ttcggacgta atcgctcgga acgaaggtct taggatcttt gttccgagct
    30601 tttttattta ttgcaggatc aggaaaaaag acaggagtat tataaataga agaaacacga
    30661 aataaaagag tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt ttttacaaga
    30721 aaaagccttg ctttgtgagt ttatatatat tataatgaca aaggtgcgtt tactttagtg
    30781 aattgcacag acgtaatcag aatgatttcg tcggctaaga aacgagaggt tgcgacacgc
    30841 ccggacgctt tgccacgaac gagcgtgacg gaaattttcg tggagctatg tctacttttc
    30901 aaaataggcg aaggagggaa caagatggca aaacaaaaaa ttcgtatccg tttaaaagcg
    30961 tatgaacacc gtattttaga tcaatcagcg gataaaatcg tggaaactgc aaaaagaact
    31021 ggagctagcg tgtctggtcc aattccatta ccaactgaac gcagtcttta cacaatcatc
    31081 cgtgcgactc ataaatacaa agattcacgc gaacaattcg aaatgcgtac acacaaacgt
    31141 cttatcgaca ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa gctagactta
    31201 ccatctggtg taaacatcga aatcaaatta taaaactaaa acaaaaataa tggaggtgta
    31261 ctcatgacca aaggaatctt agggaaaaaa gtgggaatga cacaaatctt cactgaatct
    31321 ggtgaattaa ttccagtaac tgttgtagaa gctactccaa acgtagtact acaagtgaaa
    31381 acaatggaaa cggatggcta cgaagctatt caagttggtt accaagatat gcgtgaagtt
    31441 ttatcaaaca aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc taagcgcttc
    31501 attcgtgaat tcaaaaatgt tgagctagga gaatatgaag taggaaaaga aattacagta
    31561 gatgttttcc aagcaggaga cattattgat gtaacaggta ctacgaaagg taaaggattc
    31621 caaggggtta tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg ttctcgttac
    31681 catcgtcgtc ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa aaacaaacgt
    31741 cttgctggcc gtatgggtgg caaccgcgta actatccaaa acttggaagt tgtacgtgtc
    31801 gatgcagaaa gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa aaaatcttta
    31861 attactatca aatcagctgt gaaagctaaa taattgtagg aagaaaggag gaactaagga
    31921 atgccgaatg tagcattatt caaacaagat ggaagccaaa acggtgaaat cacacttaac
    31981 gaagaaatct tcggaattga acctaatgaa agtgttgttt acgatgcaat cgtaatgcaa
    32041 cgtgcgtcat taagacaagg aacacacgcg gttaaaaacc gtagcgctgt tcgcggcggc
    32101 ggacgtaaac catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc tatccgctca
    32161 ccacaatggc gtggaggtgg cgtagtcttt ggaccaacac cacgttctta cagctacaaa
    32221 cttcctaaaa aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa agttgctgaa
    32281 aacaacttgg tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa agaattcaaa
    32341 caagttcttg ctaacttatc tatcgactct aaagtattgg ttgttctaga aaaaggtaac
    32401 gactttgctg ctttatcagc acgtaactta ccaaacgttt ctgtagtaac ttctgataat
    32461 gtgagtgtgc tagacgttgt atcaaataca aaagtgttag caacacaaac tgctcttact
    32521 caaattgagg aggtgcttgc ataatgaact tactagacgt aatcaaacgc ccagtgatca
    32581 ctgaaaaatc aatgcttgcc atggatgaca agaaatacac ttttgaagtg gacactcgcg
    32641 caaacaaaac tttagtaaaa caagcggtag aagctgcttt tgacgttaaa gtcaaaaacg
    32701 tgaacatcgt gaacgtacgt gcgaaattca aacgtatggg caaatatgca ggatatacaa
    32761 aaaaacgtcg caaagcgatc gttacattga ctgaagattc aaaagaaatc caattattcg
    32821 aagctgctga ataagcacta atagataact cattaaatta ggagggaaaa acgtggcgat
    32881 taaaaagtac aaacctacct caaatggtcg tcgtaacatg acaggttctg attttgctga
    32941 aatcacaaca tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc atgctggacg
    33001 taacaacaac ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac gccaatatcg
    33061 cgtgatcgac ttcaaacgta acaaagataa cgttgttgca actgttcaaa cgatcgaata
    33121 tgatccaaac cgttcagcta acatcgcgtt agtacactat gaagatggag tgaaagctta
    33181 catcttagca cctaaaggac tagaagtggg tatgaaactt gtttcaggtc cagaagcaga
    33241 tatcaaagtc ggaaatgctc ttccattaga aaacattcca gttggtactg ttatccacaa
    33301 cattgaaatg aaacctggta aaggtggaca attgatccgt tctgctggta caagtgctca
    33361 agtacttggt aaagaaggca aatacgtatt gatccgcttg aactctggtg aagttcgtat
    33421 gatcttagct gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac acgaattaat
    33481 caacatcggt aaagctggac gctctcgttg gatgcgtaaa cgcccaactg tacgtggtag
    33541 cgtaatgaac cctaatgatc acccacacgg tggtggtgaa ggtaaagctc ctatcggacg
    33601 taaagcacca gtttctcctt ggggtcaacc agctcttggt tacaaaactc gtaacaaaaa
    33661 agctaaatca gacaaactta ttgttcgtcg tcgcaaaaat aaataattac tttgaccatg
    33721 tgtcgcacat tttgagagga ggttcaccat gggtcgtagt ttaaagaaag gaccttttgt
    33781 cgatgagcat ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga aaaaagttat
    33841 caaaacttgg tcacgccgtt caacagtttt cccaagtttt gttggatata ccatcgcagt
    33901 ttatgatgga cgtaaacatg taccagttta catccaagaa gacatggtag gacacaaatt
    33961 aggtgaattt gcacctacaa gaacttatcg tggacatgct gctgacgata agaaaacgaa
    34021 acgctaattt gaggggagga taaatccaaa tggcagaaca aatcacatca gctaaagcaa
    34081 ctgcaaaaac agttcgcact tcacctcgaa aagcccgttt agtaattgac cttatcagag
    34141 gtaaaagtgt cgcagatgca atttcaatct tgaaattcac accaaacaaa tctgctggaa
    34201 tcattgaaaa agtattgatg tcagcagttg cgaatgcaga aaataacttt gacttagatg
    34261 ttgaaaactt ggtagtaagc gaagcttttg ttaacgaagg acctacaatg aaacgtttcc
    34321 gtcctcgtgc aaaaggatca gcttctccaa tcaacaaacg tacaagtcat atcacggtag
    34381 tcgtatcaga aaaataagga gggataatca gtgggtcaaa aagtacatcc aattggaatg
    34441 cgtgtaggca tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga gtatgctgag
    34501 ttcttacacg aagatttaag aatccgtaaa tttatttcaa ctaaacttgc tgatgctgct
    34561 gtatcaacag ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat tcacacagct
    34621 aaaccaggta tggttattgg taaaggcggt tctgaagtcg aaagcctaag aaaagaatta
    34681 aacaaattaa ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa accagatcta
    34741 gatgcaaaat tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt tgctttccgt
    34801 cgcgctcaaa aacaagccat ccaacgctca atgcgtgctg gcgctcaagg gatcaaaact
    34861 caagtatctg gtcgtctaaa cggagcagat atcgctcgtt cagaaggata ctcagaagga
    34921 actgttcctc ttcatacttt acgtgcggac attgattacg catgggaaga agcagataca
    34981 acatacggaa aactaggagt taaagtgtgg atctatcgtg gagaaattct tccaactaaa
    35041 aaaaacactg agaaaggagg gaaataatca tgttagtacc taaacgtgta aaacaccgtc
    35101 gtgaattccg cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta gcattcggtg
    35161 aatacggttt gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc gaagctgctc
    35221 gtatcgcaat gactcgttac atgaaacgtg gtgggaaagt atggattaaa attttccctc
    35281 acaaatctta tactgccaaa gcaattgggg tacgtatggg ttctggtaaa ggggcacctg
    35341 aaggatgggt tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca ggcgttcctg
    35401 aagaagtagc tcgtgaagcg ttacgtctag cttctcacaa attaccaatg aaaactaaga
    35461 tcgtaaaacg tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat cagagaatta
    35521 accactgccg aaatgcttga tcaagaaaaa caattaaaag aagaattgtt taatcttaga
    35581 ttccaattag caacaggtca attagaaaac actgcacgta ttaaagaagt acgtaaatcg
    35641 attgcacgca tcaaaactgt gttgcgtgaa caagctaagt aattatggaa ggaggccatt
    35701 aagcgtatga ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt ggtttcagac
    35761 aaaatggata aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc tatctatggc
    35821 aaacgcatga actattctaa gaaatacaaa gtgcatgatg aaaacaacac agcaaaagtt
    35881 ggcgacatcg tgagaattat ggaaactcgt ccattatcag ctacaaaacg tttccgtttg
    35941 ttggaagtag tcgaagaagc agttattatc taataacacg agattttcct atatagattg
    36001 aatgcaaaat ctgaaaggag gatacacaac gtgatccaag cagaaagtcg tttaaaagtc
    36061 gctgataact caggcgcacg tgaaattttg acgatcaaag tccttggtgg atctggtcgt
    36121 aaaactgcta acatcggtga tgtgatcgtt gctacggtta aacaagcaac gccaggtggg
    36181 gttgtcaaaa aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc aggagctcgt
    36241 cgtacagacg gttcatatat taaatttgat gaaaatgctg cagtgattat tcgtgatgat
    36301 aaaagccctc gtggaacacg tatctttggg ccagttgcac gtgaactacg tgaaaacaac
    36361 ttcatgaaga tcgtttcact agcaccagaa gttttataat cttgagacac ttatcaaagg
    36421 aggtgcgaaa cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat cactggaaaa
    36481 gacaaaaaca aagaaggcgt tgtactagca gcgttcccga aaaaagataa agttattgtc
    36541 gaaggtgtaa atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc tcaaggtgga
    36601 attgttgaga tggaagcgcc aattcatgtt tctaacgtga tggtggttga ctctactggt
    36661 gtagctggcc gagttggcta caaagaagta gacggcaaaa aagtccgtgt ttctaaaaaa
    36721 accggtgaag ttttagataa ataaaagttt aaggaaggag ggtcttactg aatgaaccgc
    36781 ctaaaagaaa aatatcttaa agaagtaact ccatcattga tggaaaaatt taactatagc
    36841 tctgttatgc aaacacctaa agttgaaaaa atcgtcatca acatgggtgt tggtgatgcg
    36901 gtatcaaacg ctaaaaactt agataaagca gttgaagaat taactttgat cactggtcaa
    36961 aaaccaatga ttacaaaagc taaaaaatca atcgctggat tccgtttacg tgaaggaatg
    37021 ccaattggtg caaaagttac attgcgcgga gaaagaatgt acgaattctt agacaaattg
    37081 gtttctgttt cactaccacg tgtacgtgac ttccatggtg taagtaaaaa agcctttgac
    37141 ggtcgtggaa actatacttt aggtattaaa gaacaattga tcttcccaga agtagattat
    37201 gatttagtag ataaagtacg tggtatggac atcgttattg taacaacagc aaactctgat
    37261 gaagaatctc gtgagttgtt gacacaatta ggcatgccat tccaaaaata attaaaggag
    37321 gcgaactacg tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc aaaattttca
    37381 acacaagctt atactcgttg cgaacgttgc ggacgtccac attcagttta tcgtaagttc
    37441 catctttgcc gtatttgctt ccgcgaactt gcctataaag gtcaaattcc cggcgtgaag
    37501 aaagctagct ggtaaaaagt aaattcgcat aagaaggagg taaaagatca atggtcatga
    37561 cagatccaat tgcagatttt ctaactcgta tccgtaacgc aaacatggtt aaacatgagt
    37621 ctttagaagt acctgcatca aaaattaaac gtgacatcgc tgaaatcttg aaacgtgaag
    37681 gtttcgttcg tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc cgtgttttcc
    37741 taaaatttgg taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc tctaaaccag
    37801 gtttgcgtgc ttatgtcaaa gctgacgaag taccaaaagt attgaacggt ttaggtatcg
    37861 caatcatttc gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa aaaaatatcg
    37921 gcggcgaagt tatcgcttac gtatggtaat caagtagaat ctacaaggag gtgtctttaa
    37981 gtgagccgta tcggaaataa agtcgttgtt atccctgaag gagtaacagt tactcaagac
    38041 ggaaacaacg ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt ttcagcagat
    38101 atcaaaatga atatcgaagg aaacgaagta actttcactc gtccaaacga cagtaaagaa
    38161 atgaaaacaa ttcatggaac aactcgtgcg aacttcaaca acatggttgt aggtgtcagc
    38221 gaaggtttcc aaaaagcgtt agaactaatc ggggttgggt accgtgcaca attacaagga
    38281 tcaaaacttg tattgaacgt tggttactca catccagtag aaatcacacc accagctgga
    38341 gtaactgttg aagtaccagc taacacacaa gtaattgtta aaggtgcgaa caaagaagaa
    38401 gttggcgaat tagctgcaaa cattcgtgga actcgtcctc cagaacctta taaaggtaaa
    38461 ggtattcgtt atgttggcga atatgtacgc cgtaaagaag gtaaaactgg taaataatcg
    38521 tttagaaaaa ttgcatagaa gccagcagca ggaaaaaggc atttggctga agtttacctt
    38581 caaaaaaata catttttact tgtgccgcag cttctatttt tcgaaacgta tttactgctt
    38641 gaaatagtag tacaataaaa ataaagaggt gacaattgtg attacaaaac cagataagaa
    38701 caaaacacgt caaaagagac atcgtcgtgt acgtaacaat atctctggta ctgctgagcg
    38761 cccacgcttg aacgtttttc gttctaacaa aaacatctac gctcaaatta ttgatgacgt
    38821 agcgggtgta acgctagcaa gtgcctctac cttggataaa gaaatttcag gtggaacaaa
    38881 aacagaagca gcacaagctg tcggtaaact agttgctgaa cgtgcagctg aaaaaggcat
    38941 taaagtagta gtctttgacc gtggtggata cctttaccat ggccgtgtgc aagctttagc
    39001 tgaagctgct cgcgaaaatg gactagaatt ttagaagaag gaggaatacc attcatggtt
    39061 tatatcgatc caaaacattt ggaattagaa gaccgcgttg ttgcaattaa ccgtgtaaca
    39121 aaagttgtta aaggtggacg tcgtctacgt tttgctgctt tagttgttgt cggtgacaaa
    39181 aacggacacg tagggtttgg tacaggtaaa gctcaagaag ttcctgaagc aatccgtaaa
    39241 gcaattgaag atgcgaagaa aaacttagtt gaagtaccta tggttggttc aacaatccca
    39301 cacgaagtaa tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc tgtagaaggt
    39361 tctggggttg ctgctggtgg accagttcgt gccgtattag aattagctgg ggtagcagat
    39421 atcacttcta aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc aacagttgaa
    39481 ggtttgaaac aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa atcagttgaa
    39541 gaattaatcg gataaggagg acaacaataa tggctgaatt aaaagttact ttaaaacgca
    39601 gtattatcgg acgtcctcaa aaccaacgcg atacagttaa agcgttaggt cttacaaaga
    39661 ttaacagttc agtggttaag ccttctaatg aagcaattaa aggcatgatc aatactgttt
    39721 ctcacctagt ggacgttgaa gaaatttaat ttagaaaaat cataactaga acattaagga
    39781 ggtgccaaac ctatgaaact tcatgaatta aaacctgctg aaggttcacg ccaagtacgt
    39841 aaccgtgttg gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg cggacaaaaa
    39901 ggacaaaaag ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg acaaacacca
    39961 ttgttccgtc gtttaccaaa acgaggattc acaaacgtga accgtaaaga ctatgcagtc
    40021 attaatcttg atgtattgaa ccgctttgaa gacggagctg aagtaactcc tgttgcttta
    40081 gttgaagctg gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc taatggggaa
    40141 ctaaacaaga aattaactgt gaaagcagca aaattctcta aagcagctca agaagcaatt
    40201 gaagcggctg gtggatcaat cgaggtgatc taatgtttag actattaaaa gatgctttta
    40261 aagtaaaaga cattagatcg aaaatcctat ttactgtatt tgtgttgttt gtatttcgcc
    40321 taggtgcaca tattactgta ccaggcgtaa atgctgcttc attgcaaaac ctttcttctt
    40381 tgccattttt caacatgttg aacttggtaa gtggtagtgc gatgcaaaac ttttccattt
    40441 tctcaatggg ggtttcacca tacattactg cttctatcgt cattcagctt cttcaaatgg
    40501 acatcgtgcc tcgatttgtt gaatggtcga aacaagggga agttggtcgt aaaaaactga
    40561 atcaagcgac tcgatatctg acattagtat tggcatttat ccaatctgtc ggtatcactg
    40621 ctggattcaa tcaatacgct caattaggtt tcgtgaataa tcctaacgta gcaacttacg
    40681 taacgatcgg tttgattcta acagccggta cgatgtttgt tacttggcta ggagaacaga
    40741 tcacagaaaa aggtatcgga aatggcgtat caatgatcat ttttgcaggt atcatttcta
    40801 gattgcctga aagtatcaga gaaatttatg aagattactt tataaatatt gatccttcag
    40861 acatatggaa atctgctatt tttgtggcca ttttactagt agcggtcttg ttgatcgtga
    40921 cattcgtaac atttttccaa caagctgaac gcaagatccc gatccaatat acgaaacgcg
    40981 tagctggcgc gcctacaagc agctatctgc cgttgaaagt aaatgcagct ggagttattc
    41041 cagttatctt tgctagttcg tttattgcta cgccaaatgc tgttttacaa gcattgagcg
    41101 gtagattcgg ttctgaaaac tggtacaaag tgatgatgga gatctttaac tataatacag
    41161 cacctggtgc tacgatttat acgattctga tcgttgcctt cactttcttc tacgcctttg
    41221 ttcaagtaaa ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt tatataccaa
    41281 gcgtacgccc tggtaagggg actgaagagt atgtctcaag attactgatg cgtttaagta
    41341 cagttggtgc agtcttcctt ggtttagtgg cattgcttcc aatcattgct caaatggttt
    41401 gggatcttcc acaatcaatc ggattaggcg gtacaagctt actgatcgtt atcggtgtag
    41461 ctttagaaac tgcgaaacag ttagaaggct taatgctgaa acgtaaatat gttggattca
    41521 ttgagtaaga aagcgtgttg aggtttcccc tcaacacacg ttctcacaca tttattagga
    41581 gggaaaacaa tgaacctcat tttaatgggg ctgccaggcg caggtaaagg aacacaagct
    41641 gaaaaaatca ttgatgctta tggtattccg cacatctcaa caggagatat gttccgcgca
    41701 gcgatgcaaa acgaaacagc tcttggcctg gaagcaaagt cttatatgga taaaggtgca
    41761 ttagttcctg atgaagtaac aaatggaatc gtgaaagagc gactagctga accagataca
    41821 gagaaaggct tcttattaga tggctttcca cgtacgctag atcaagcaaa agctctggac
    41881 gcaatgctaa aagatttgaa taaaaaaatt gatgctgtca tcgatatcca tgttggtgaa
    41941 gaaatcttgg tcgaacgtct agccggccga tttatctgca gcaattgtgg agcaacttac
    42001 cataaaattt tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg tgggcatgaa
    42061 ttctatcaaa gagaagatga taaacctgag acggttaaaa atcgtctggc tatcaacatc
    42121 aaaaatagtg aaccaatctt ggcttattat aaagaacaag gtttgctaaa cactattgat
    42181 ggtgctcgtg aaatcgatgc tgtatttgca gatgtacaaa aaatcattga aaaatagcac
    42241 gattgcccgt agccgacttg cattatgcag ttactcttgt caagttcaat aaattatgat
    42301 agaatatgtc agtccgactt ctagagaatc ctctagatga tccattttga gaattttgag
    42361 gtgggaactg cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt acaaggaggt
    42421 actgtgcgtg gcaaaagaag atatgattga agtcgaaggt acagtcgtcg aaactttgcc
    42481 gaatgcaatg tttaaagttg aactagaaaa cggacaccaa gttcttgcta ctgtttcagg
    42541 taaaattcgt atgcattaca tccgtatctt acctggtgac aaagtaacgg ttgaattgtc
    42601 tccatacgat cttaaccgtg gccgtattac ttatcgcttt aaataattgt actccgtaaa
    42661 actacaggag gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg cgaacattgt
    42721 aaagtaattc gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc aaaacataaa
    42781 caacgtcaag gataattgga ggtgctataa atatggctcg tattgcagga gtagatattc
    42841 ctcgtgataa acgtgtagta gtttcactta cttatatcta tggtattggt aacactactg
    42901 ctaaaaagat cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt gaattaacga
    42961 atgaacaaac agatgctatc cgtgcagaag tagataaatt aaaagttgaa ggggatcttc
    43021 gtcgtgaagt gaacttgaac atcaaacgtt tgatggaaat cggttcttac cgtggaatcc
    43081 gtcatcgccg tggattgcct gttcgtggac aaaacacgaa aaacaatgca cgtactcgta
    43141 aaggcccagc tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa ggaggttaaa
    43201 acttcatggc agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat atagaagcag
    43261 gtattgcaca tatccattct acattcaata acactatcat catgatcact gatactcacg
    43321 gaaatgcatt agcgtggtca tcagctggtt cattaggttt caaaggaagc aaaaaatcaa
    43381 ctccttttgc cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg gaacacggat
    43441 taaagactgt tgaagtaaca gttaaaggac ctggttctgg acgtgaagca gcaattcgtt
    43501 cattacaagc tacaggttta gaagtgactg caattcgtga cgtgactcca gttcctcata
    43561 atggtgcccg ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt ttgagtttta
    43621 tcagtaacgt cattgaacaa gacacttttc gttttgaaag gggtaaagat aagaatgatt
    43681 gagttcgaaa aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg caagttcatc
    43741 gttgaacctc ttgaaagagg gtatgggact actttaggga attccctacg tcgtatttta
    43801 ttatcttctt tgccaggtgc tgccatcact aatattcaaa tcgatggtgt actgcatgaa
    43861 ttctctacca tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa cattaaaggc
    43921 ttagcgctga aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat taccggacct
    43981 gctacagtaa ctgctggcga tattatcgtt gatagtgatg tagaaattct taacaaagat
    44041 atgtacatct gtagtgtctc tgaaggagct acgttccatg ctcgcttaac agtgaaacct
    44101 ggacgtggtt atgttcaagc agatgaaaat aaaaaagaag atatgccaat tggcgttctt
    44161 ccagtcgatt caatctacac acctgttcgt cgtgtgaact atcaagttga gaacacacgt
    44221 gttggacacc gtgaagattt tgataaattg acgatggaga tttggacaga tggttcaatt
    44281 gaaccgttgg aagcaatgag tttgtctgcg aaaatcatga ctgaacattt ggatattttt
    44341 gtgaacttaa ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga agaaacacaa
    44401 aaagaaaaaa tgttggaaat gacgatcgaa gagttagact tgtctgttcg ctcttacaat
    44461 tgtttgaaac gtgcaggaat caatactgta caagaactta caaataaatc tgagcctgaa
    44521 atgatcaaag tccgtaactt aggacgtaaa tcattagaag aagttaaagc aaaattgcac
    44581 gacttaggtt taggtttacg taaagaagat taaaaccttt tacgaaggag ggaaaccaac
    44641 gtgagttatc gtaaactagg acgcacatct agccaacgta aagcgatgtt gcgtgattta
    44701 acaactgatt tattaatcaa cgaacgtatt gtgactactg aagctcgtgc gaaagaagtt
    44761 cgctcaactg cagaaaaaat gatcacttta ggtaaacgcg gagatttgca tgcacgtcgt
    44821 caagctgcgg ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa tgaggacatc
    44881 gtcattgaat cagctttaca aaaactgttc aacgatattg cacctcgtta cgcagaacgt
    44941 caaggcggat acacacgcat tttgaagaca gaaccaagac gcggtgatgg tgcaccaatg
    45001 gttgtcttag aacttgtcta aaatcacact aataacatca ctttatagat gattctttta
    45061 gagtgttatg atgttggaag atatttttcc gagtctagct cagcgctgct cctcaagcga
    45121 atatcgcttg gggagtaagc actcatcata aagtgttttt ttataccttc aattacctgt
    45181 catgacagat atgtttgaag gcataaaaaa acattttaag cgagaaataa ttgaaaattt
    45241 tgtctttttt tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa tcatggagcc
    45301 aattattgaa ttagaaaaga taaattataa ataccagcca gatgatcttc gccccgcatt
    45361 gaaagatgtc tcctttacaa ttgataaagg tgagtggatc gctattattg gacacaatgg
    45421 ttcaggaaaa tcgacacttg ccaaaacgat caatggattg cttctgccag aatcagggat
    45481 cgtgaaagtg ggaaatcaaa tattggatga agaaaatatt tggacgatcc gacagatggt
    45541 tggaatggtt tttcaaaatc cagacaatca gtttgttggt tcgacagtag aagatgacgt
    45601 tgcttttggt cttgaaaatc aagggattcc cagagaagaa atgctagtgc gtgtgaaaga
    45661 tgcacttgag aaagttagaa tggctgagtt tgcttcacgc gagcctgctc gtttatcagg
    45721 agggcaaaaa caacgtgttg ccattgccgg cgtggttgcc ttacgacctg atatcattat
    45781 tttagatgaa gcaaccagta tgctagatcc agaaggtcgt gaagaagtga tctctacgat
    45841 caagaagatc aaagaagaaa gtcaattgac cgttatatct atcactcatg atattgatga
    45901 agcagctaat gccaatcgga tactagtgat gagacaaggc gaacttgtcc gtgaaggtac
    45961 accaaaagaa attttttctg ctggtcctga actgatcgac ttaggtctag atctaccatt
    46021 tcctgaaaaa ctgaagagcg cactgaaaga acgtggggtt gatgtgccca gtgaatatat
    46081 gacagaagaa aggatggtgg attggttatg gacatccgtt ttgaacaagt agacttcact
    46141 tatcagccga atacaccatt tgaacaaaga gccttatttg acatcaatat gacgatcaaa
    46201 gaaaacagtt atactgcatt agtagggcat accggaagcg ggaaatcaac cttacttcag
    46261 catttgaatg cgcttgttaa gccaacaagc ggtacagtcc atattggaga acgagatatt
    46321 cagccggata cggataataa gaatttaaag cccatccgaa aaaaagttgg catcgtcttt
    46381 cagtttccag aagcacagct attcgaagaa acggtagcaa aagacattgc tttcggtcct
    46441 aaaaactttg gtgtcagcga agaagaagca ctagtcctag caaaagaaac attagaacaa
    46501 gttgggctgg atgaaagcta tttggaacgt tcgccatttg aactttcagg agggcaaatg
    46561 cgtagagtag caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt attggatgaa
    46621 ccaacggcag gacttgatcc ccagggtaga aaagaaatga tggagatgtt ttggcgtctt
    46681 cataaggaac agcagatcac tattgtatta gttacgcatt taatggatga tgtagccaat
    46741 tttgcagatt atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg ggagccgcaa
    46801 gaggtctttc aaaacattga atggctaaaa gaaaaacaac taggcgtacc tactgccacc
    46861 gagtttgcag aagaattaat ggctaaaggg atgaactttg ctaccttgcc tttgacagct
    46921 gaagaattag cagacgctat cgtccattat gctggaggaa atgcccatga tgaataaatt
    46981 gatatttggt cgttatatgc ctggagattc cttgatccat cgattggatc cccgagctaa
    47041 attattagcc agtttttatt ttatcggaat cattttttta gcaaataatt gggaaacgta
    47101 ccttttgttg gcatttttca cattactttc tgtcttcttg tcaaaagtag atttgaaatt
    47161 ttttatccga ggcattcgcc cattgatctg gctaatcctc ttcactgtag ctttgcaggt
    47221 cttgtttaca agtggcggag aaacttattg gagttggggt gttttccata tcactgaatt
    47281 tggtgtgatg aacggcctct ttattttctg tcgattcgtg ttgatcattt ttatgtcgac
    47341 tctactcacg cttactacac cgcctttgga attatcggat gcgattgagt acttgcttcg
    47401 tccattgaaa gccgtacggt tcccagtcca cgaaatttca ttgatgctgt cgattgctct
    47461 tcgttttgtg cctacattga tggacgaaac agaaaagatc atgaacgctc agcgcgctcg
    47521 cggtgttgat tttggtgaag gaaacctagt tcagaaaatg aaagcagtca ttcctttgct
    47581 gattccttta tttgtcagca gcttcaaccg tgcggaagat cttgcgactg ctatggaagc
    47641 aagaggctac aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt ggcataatag
    47701 agacacgatg gttatcattg cttttgtatt attaactgtg ctactgatct ttttgagagg
    47761 ataaacgttc agtgggccgc tatctattct tttcttgctg atctgtttgc aagaaaggat
    47821 gcgagcagtg tatgacaaga aatattaggg tctgtggcag actaaggtca caggcctctt
    47881 tcttttatct gaaaagaaag aacgaaaaaa ttttataaaa aaatcattat tccagataac
    47941 cttttaggaa caaaggagag aaaaacatgg tacgttacaa agcaatcatt gcatacgacg
    48001 gaacaaattt taatggattc caaaaacagc caaatgggcg tactgttcaa gaagaagtag
    48061 aaaaaacatt acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt tcgggaagga
    48121 cagatgccgg agtacatgcg attgggcaag taatccattt tgattatcca gaggagcgtc
    48181 cgttagaacg aatgcgtttc gcgctagata cccagtcacc agaggatatt gctgtaagac
    48241 aagtagaaat tgtttcagaa gacttccacg caagatattt ggtcaaagaa aagacgtatc
    48301 aatttcgagt ggatataggg aaaccacgca gtccttttcg acgccattat gcaagttatt
    48361 ttccttaccc tcttgatttg gaaaaaattc agcgggcgct tcctgattta ttagggactc
    48421 acgatttcac ctctttttgt gcatcaggaa gctcgataga ggataaagta cgtacgatct
    48481 acgaggctaa gatggaggta aatgaaacaa aagacgaatt gttgtttaca tttaggggaa
    48541 atggcttctt gtataagatg atacggatct tagtaggaac gcttttgaaa attggcaatg
    48601 gccgattgcc tgaagatagt atcccagata ttattgcgaa aaaagatcgt aatgcagcag
    48661 gacctacagc acatcctgaa ggactttatt tgtatgaagt gaaatatgaa taagaggttg
    48721 tgacagaagt ggtcagcttc aagaaataag aaggaattta cgaaaattac ttctcaaaat
    48781 tttgcaaaat ttcggcttat tttcgaagga gctacttctg gaacaccgtg aatcagaggt
    48841 tgtgaaaaag ctgccctgca tcaagtaata agaacaacca aacaaaaata gctcgtcata
    48901 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag
    48961 aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaacggaaa aatagttcct
    49021 catattttgc cgtttgttga gctaataccg aaggtcagct gattgaacac cgtttaagaa
    49081 agaggtcgtg agcctgtcat ttaactctga gtcacaagta acaatttcaa aaaacagctt
    49141 ctcatctttg atgaatatcg tgacttgtgg acgaggcgtt gataagtgaa tcatcttttt
    49201 gttacctatt ttttctgtaa ccaaaaaacg atggaacgtt gttttgattg cccttaatga
    49261 ggacagtcgg aacaggtttg tcatttttat atttaaatgg cacaaatttt tctaattatt
    49321 tgtcatttag cgctgaaaat ttttgctatt tatctaaaaa agtgataact acttaagatg
    49381 aagcagaata tttacgccat acggcataca accgcgaaaa atataaaaaa aagaaaacga
    49441 ttgaacgcgt ttttgcagtc gcaaaggaaa agcatggaat gcgctacatc aagtatcgag
    49501 acttggaaaa caatcaggtg tatacgctga ttgtttttgc tgcaatgaat ctgaaaaagt
    49561 tgtccacttg gcagttggaa catatgtctt catatctttt attatacaaa aatagcaaaa
    49621 gcaccagtct acactcttag taaagaggaa actgatcctt ttgtctacat tcttaaagta
    49681 cttaaactaa gtactttatt ttactcttta ggattgatac agtagcatct atccctttat
    49741 taataatctt ttattttttt aaataaagat gttgtctcca tcttcaggca ctaaaacttt
    49801 ttcggaaatt cctttttctt tattgaaaga ctttaattct tctcttgaca gtccccaatg
    49861 gtttacagct tccatatgaa cggcaataat tgtggaatct ggtattgctt tatgcaattc
    49921 atataaatcg tctttcccca taataagaga ttctccaact aaaaaatgat tatctccagc
    49981 atttgctact atgatttctg gattatactg atcaatagtt tgtttaacag cttcatacca
    50041 aactgtatct cctgtaaagt atagtgtctt ctcagtcgaa tgtttgaaaa caataccgca
    50101 aacattacct acataattca agatttctcc tctgccatgt ttgccttttg ttttaaccaa
    50161 ttgtatagat tcaaaaactg tgtcttctcg tagtacctcc acatttttaa aaccatatag
    50221 tcgaatttca ttcgcatcat tttcatcttg cacaaataat tttatttctt taggtaggac
    50281 ctctttcgct atatcgtccc aatgatctag atgtaaatgt gttacaataa cagcatcaat
    50341 accgttgata atatcttcga ctggcattgg caaatcaact agaggattca tttgatcttt
    50401 tcttggtgat ggaaaaggac ctttcgaaat aaatggtgga agtgttcctt tttccgctaa
    50461 cattggatca attaaaaatt ttttacctcc atagtctaag atagctgttg cattacgaat
    50521 gtgatgtata ttcataaacg ttgtctcctt tttgttgata tgaaatattt ttagtataat
    50581 tcaattccat acaataaata aaccgaacaa aaaaagtctt ttactatttt tacttaattt
    50641 atgaaaggtt gtttaccatg ttagaccaaa cagatataaa tatcattgag gaactatcaa
    50701 aaaatagtcg aatcacaatg aaagaacttg gagaaaaagt acatttgact ggacccgctg
    50761 tctcggcgag agtgacaaaa ctagaggaaa gtggagttat tgaaaattat acagtaaaat
    50821 taaatatgga aaagctaggg ttttcgattc acgctttttt aattataatt acgcaaaatt
    50881 taaatcataa accctattta tcatttatta aaaatcatga aaaattcttg attagaaatt
    50941 ataagattag tggagggggc tgttatcttt tggaatgtag atttacttca aaaaaatcaa
    51001 tgaacagctt tttggaacag cttaatgaat atgcgaatta taaattatct attgttatag
    51061 attaataata aa
//
LOCUS       DAZAID010000011        47345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000011 DAZAID010000000
VERSION     DAZAID010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.011"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..807)
                     /locus_tag="ACG9BE_000564"
     CDS             complement(<1..807)
                     /locus_tag="ACG9BE_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN3031344.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKK"
     gene            complement(1031..2971)
                     /gene="thrS"
                     /locus_tag="ACG9BE_000565"
     CDS             complement(1031..2971)
                     /gene="thrS"
                     /locus_tag="ACG9BE_000565"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFN3031345.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3308..3487)
                     /locus_tag="ACG9BE_000566"
     CDS             complement(3308..3487)
                     /locus_tag="ACG9BE_000566"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFN3031346.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3534..5357)
                     /locus_tag="ACG9BE_000567"
     CDS             complement(3534..5357)
                     /locus_tag="ACG9BE_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN3031347.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5635..7914
                     /gene="helD"
                     /locus_tag="ACG9BE_000568"
     CDS             5635..7914
                     /gene="helD"
                     /locus_tag="ACG9BE_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFN3031348.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8007..8653)
                     /locus_tag="ACG9BE_000569"
                     /pseudo
     CDS             complement(8007..8653)
                     /locus_tag="ACG9BE_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8832..9551)
                     /locus_tag="ACG9BE_000570"
     CDS             complement(8832..9551)
                     /locus_tag="ACG9BE_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3031349.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9682..10833)
                     /locus_tag="ACG9BE_000571"
     CDS             complement(9682..10833)
                     /locus_tag="ACG9BE_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFN3031350.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11050..11790)
                     /locus_tag="ACG9BE_000572"
     CDS             complement(11050..11790)
                     /locus_tag="ACG9BE_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3031351.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11794..12117)
                     /gene="rsfS"
                     /locus_tag="ACG9BE_000573"
     CDS             complement(11794..12117)
                     /gene="rsfS"
                     /locus_tag="ACG9BE_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFN3031352.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12174..12767)
                     /gene="yqeK"
                     /locus_tag="ACG9BE_000574"
     CDS             complement(12174..12767)
                     /gene="yqeK"
                     /locus_tag="ACG9BE_000574"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFN3031353.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12751..13401)
                     /locus_tag="ACG9BE_000575"
     CDS             complement(12751..13401)
                     /locus_tag="ACG9BE_000575"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFN3031354.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13411..13722)
                     /gene="yhbY"
                     /locus_tag="ACG9BE_000576"
     CDS             complement(13411..13722)
                     /gene="yhbY"
                     /locus_tag="ACG9BE_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFN3031355.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13738..14847)
                     /gene="yqeH"
                     /locus_tag="ACG9BE_000577"
     CDS             complement(13738..14847)
                     /gene="yqeH"
                     /locus_tag="ACG9BE_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFN3031356.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14844..15371)
                     /locus_tag="ACG9BE_000578"
     CDS             complement(14844..15371)
                     /locus_tag="ACG9BE_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFN3031357.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15551..16336)
                     /locus_tag="ACG9BE_000579"
     CDS             complement(15551..16336)
                     /locus_tag="ACG9BE_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFN3031358.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16329..17198)
                     /gene="accD"
                     /locus_tag="ACG9BE_000580"
     CDS             complement(16329..17198)
                     /gene="accD"
                     /locus_tag="ACG9BE_000580"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFN3031359.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17199..18578)
                     /locus_tag="ACG9BE_000581"
     CDS             complement(17199..18578)
                     /locus_tag="ACG9BE_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFN3031360.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18586..19005)
                     /gene="fabZ"
                     /locus_tag="ACG9BE_000582"
     CDS             complement(18586..19005)
                     /gene="fabZ"
                     /locus_tag="ACG9BE_000582"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFN3031361.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19030..19506)
                     /gene="accB"
                     /locus_tag="ACG9BE_000583"
     CDS             complement(19030..19506)
                     /gene="accB"
                     /locus_tag="ACG9BE_000583"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFN3031362.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19509..20744)
                     /gene="fabF"
                     /locus_tag="ACG9BE_000584"
     CDS             complement(19509..20744)
                     /gene="fabF"
                     /locus_tag="ACG9BE_000584"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFN3031363.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20763..21500)
                     /gene="fabG"
                     /locus_tag="ACG9BE_000585"
     CDS             complement(20763..21500)
                     /gene="fabG"
                     /locus_tag="ACG9BE_000585"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFN3031364.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21512..22429)
                     /gene="fabD"
                     /locus_tag="ACG9BE_000586"
     CDS             complement(21512..22429)
                     /gene="fabD"
                     /locus_tag="ACG9BE_000586"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFN3031365.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22536..22760)
                     /locus_tag="ACG9BE_000587"
     CDS             complement(22536..22760)
                     /locus_tag="ACG9BE_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFN3031366.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22813..23775)
                     /locus_tag="ACG9BE_000588"
     CDS             complement(22813..23775)
                     /locus_tag="ACG9BE_000588"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFN3031367.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23785..24225)
                     /locus_tag="ACG9BE_000589"
     CDS             complement(23785..24225)
                     /locus_tag="ACG9BE_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN3031368.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24531..25496
                     /locus_tag="ACG9BE_000590"
     CDS             24531..25496
                     /locus_tag="ACG9BE_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN3031369.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25553..25783)
                     /locus_tag="ACG9BE_000591"
     CDS             complement(25553..25783)
                     /locus_tag="ACG9BE_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFN3031370.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25869..26756)
                     /locus_tag="ACG9BE_000592"
     CDS             complement(25869..26756)
                     /locus_tag="ACG9BE_000592"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN3031371.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26845..28068)
                     /locus_tag="ACG9BE_000593"
     CDS             complement(26845..28068)
                     /locus_tag="ACG9BE_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN3031372.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28085..29131)
                     /locus_tag="ACG9BE_000594"
     CDS             complement(28085..29131)
                     /locus_tag="ACG9BE_000594"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN3031373.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29295..31022)
                     /gene="ptsP"
                     /locus_tag="ACG9BE_000595"
     CDS             complement(29295..31022)
                     /gene="ptsP"
                     /locus_tag="ACG9BE_000595"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFN3031374.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31022..31288)
                     /locus_tag="ACG9BE_000596"
     CDS             complement(31022..31288)
                     /locus_tag="ACG9BE_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFN3031375.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31767..34001
                     /locus_tag="ACG9BE_000597"
     CDS             31767..34001
                     /locus_tag="ACG9BE_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3031376.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34149..34469
                     /locus_tag="ACG9BE_000598"
     CDS             34149..34469
                     /locus_tag="ACG9BE_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFN3031377.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34674..34898)
                     /locus_tag="ACG9BE_000599"
     CDS             complement(34674..34898)
                     /locus_tag="ACG9BE_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFN3031378.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35096..36676)
                     /locus_tag="ACG9BE_000600"
     CDS             complement(35096..36676)
                     /locus_tag="ACG9BE_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFN3031379.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37077..38453)
                     /locus_tag="ACG9BE_000601"
     CDS             complement(37077..38453)
                     /locus_tag="ACG9BE_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFN3031380.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38581..39669)
                     /locus_tag="ACG9BE_000602"
     CDS             complement(38581..39669)
                     /locus_tag="ACG9BE_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN3031381.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            39841..40275
                     /locus_tag="ACG9BE_000603"
     CDS             39841..40275
                     /locus_tag="ACG9BE_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3031382.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40353..40886)
                     /locus_tag="ACG9BE_000604"
     CDS             complement(40353..40886)
                     /locus_tag="ACG9BE_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFN3031383.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(40980..42158)
                     /gene="mutY"
                     /locus_tag="ACG9BE_000605"
     CDS             complement(40980..42158)
                     /gene="mutY"
                     /locus_tag="ACG9BE_000605"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFN3031384.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42151..42948)
                     /gene="recX"
                     /locus_tag="ACG9BE_000606"
     CDS             complement(42151..42948)
                     /gene="recX"
                     /locus_tag="ACG9BE_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFN3031385.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43055..44437
                     /gene="rlmD"
                     /locus_tag="ACG9BE_000607"
     CDS             43055..44437
                     /gene="rlmD"
                     /locus_tag="ACG9BE_000607"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFN3031386.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44611..45189)
                     /locus_tag="ACG9BE_000608"
     CDS             complement(44611..45189)
                     /locus_tag="ACG9BE_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFN3031387.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45511..45981
                     /locus_tag="ACG9BE_000609"
     CDS             45511..45981
                     /locus_tag="ACG9BE_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFN3031388.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46089..47315)
                     /locus_tag="ACG9BE_000610"
     CDS             complement(46089..47315)
                     /locus_tag="ACG9BE_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN3031389.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT        13485 a        10072 c         8196 g        15592 t
ORIGIN      
        1 ttttttcact ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc
       61 gatcgtagca aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt
      121 tggtctagat gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg
      181 cgtcaacgca atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc
      241 tgcacttcta cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag
      301 ttgcgggagc aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca
      361 tttttgcaga atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt
      421 ctctgccgat tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac
      481 tgtttctttt atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga
      541 ctcatttgct tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat
      601 cgtgattttg aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt
      661 cggcgcactc aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc
      721 aatattaccg ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat
      781 actttcaata cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt
      841 aagtatacca tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa
      901 tccgcccaac agaatatctt atcccatcaa tcccacagat tctattgagc atctgtgtct
      961 ttgaggaata cctgttatct attgttcgga ttgctttcta tctactactt ttttacagga
     1021 aatttaccta ttagttttct ctgctgtaat ttttgacttc tgcttccata gcatcaataa
     1081 agatattcaa atcttctaca gctgtttctt tacttccata acgacgtacg ttcactgttg
     1141 catcttccaa ttccttgtct ccaacaacta attggtaagg gatcttttgt gtttgagagg
     1201 cacgtatctt atagcccatt ttttcattac gatcatcaac ttctacacgc aaccccttca
     1261 tttgcaaacg ttctttgatt tcataagcat aatcgccatg tgcatcaaca gaaacaggga
     1321 tgattgttgc ttgtataggt gccaaccatg ttggaaaagc gcctttgtat acttctgtta
     1381 aataagcgac aaaacgttcc attgtagaaa caattccgcg gtggataact actggacgat
     1441 gcgtattttc cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc aataagaagt
     1501 ctaattgtat agttgataat gtttcttctg taccaagagc tgttttgact tgaacatcta
     1561 gttttggtcc gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat
     1621 ccatagcggc tttcagcatt gcttgggctt tttcccacat tgcgtcatca tcaaaatatt
     1681 tatctgtatt gtttggatcg cgatagctca agcggaaacg ataatctgta atattgaagt
     1741 cagcatatac tgccaccatc aattcaagcg tacgtttaaa ttcatctttg atttgatcag
     1801 gacgaacaaa tgtatgacca tcattcaacg tcatttctcg tacacgttgt aaacctgaca
     1861 aagcacctga tttttcatag cgatgcatca ttcctaattc agcgatacga attggtaatt
     1921 cacgataaga atggatcgtg tttttgtaga ccatcatgtg atgagggcag ttcattgggc
     1981 gaagaacaag catttcgcca tctcccatat ccattggcgg gaacatgtct tcttgataat
     2041 gatcccagtg tccagatgtt ttgtacaact caacatctcc cattattggt gtatatacat
     2101 gttggtaacc tagactaact tctttgtcta cgatataacg ttcgatcgtg cgtcggattg
     2161 ttgccccttt tggaagccag aatggcaaac cagaacctac ttctggggaa accatgaaca
     2221 agtcaagttc ttttcctagt ttacgatggt ctctttcttt tgcttcttca cggagacgga
     2281 tatattcttt caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt
     2341 gattgtttga gtttccacgc caataagcac ctgccactga taataattta aatacttgta
     2401 tacggccagt ggatggaaca tgtggtccac gacacaaatc gatgaattcg ccttgttgat
     2461 aagcagtaat cacttcttct tctggcaatt cactaatcag ttctactttg tacggatcac
     2521 ttgcaaaaat ttctaatgct tcctgttttg aaagaacacg gcgttcgatc ggcagatttt
     2581 ctttgacgat tttcatcatt tctgcttcga ttgccggcaa atcttctgca gttacttggt
     2641 tttgtccatt atctgtatcg tagtaaaacc ctgaatcgat agctggtcca acgccaaagt
     2701 ggatatttgg atagaggcga cgcatggctt gggccatcaa atgagctgct gaatgacgga
     2761 ctaagccaag tgcatcttca tggtctggtg tgatgatctc aatacttgca tcttcctcga
     2821 ttggacgagt caaatcaacg agttttccgt tcactttacc agctaaagct ttttttgcta
     2881 agctttttga aatgctttca gcaattgcca atgttgttgt tccagactca aattctttga
     2941 ctgcaccatc tggaaaagta atttttatca taatagtttc ctccttgaaa tgttttgatt
     3001 gatcagatca tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa gagatactag
     3061 gacgaaaagt tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta
     3121 acggtccgac cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc
     3181 ctggaaaagt ttcagcctag cttttcctct cttatcgaag actctcaaat tcagttggct
     3241 ttctcatcaa tcaattatgt gtttatttaa ccactaataa atagaaaaat caagtgggaa
     3301 actaaactta ttttttccat tccccattta caccgtatgt tccttttttc atctcattga
     3361 tgatgacatg gatgtgttct tttggtgcct gcgtgttttt atgaacagct tccgtgatat
     3421 ctttcatcat attttctaat tgttccggac tgcgaccttc tactaattcg acatgtacga
     3481 atggcataat gctctctcct ctttctgatc gttctttttt caactcgatt ctatcatact
     3541 atgtttgtct tgggtactga atcaggaaaa atggctggat ctttttttgc tctcaaggca
     3601 ctcaaaatag agaccgtatc cacgacttct tgcaaaacag ctccgaataa tgcggggatt
     3661 agtcccgtac ttgcaattaa catcaaaaaa atacaaataa agatgccaat cagtacagac
     3721 tgtttcgcta ttctcatcgt atctcttgaa atcactactg cttttgctac acgcaagaga
     3781 tcatctttca agatgaccgc atctgcactt tcactagcag ctgtagaacc atgagccccc
     3841 attgcaatac cgatatctgc agcagcaagt gctggtgcgt cattcacgcc gtctcctacc
     3901 atcgcaacag gtctttgatc tttagggata ctttctaaaa tggtcagttt atcttgaggc
     3961 aagctacggg catgtacaga atctaattgg actttttctg caatcgtttg agcaatggca
     4021 gatccatccc cggtgatcat catcaaattt ttcacatcaa gttttcttag ctccttgatt
     4081 gtctttgctg cttctgggcg aatctgatcc gaaaatagga tcgttcctgc aaaagatcga
     4141 tcaattgtta catagattgc tgtattggac gtttctattt cttcagctga aacaaaagag
     4201 gcacgtccaa tctttacttc atgattattg actactgctt ggattccttg accggtcact
     4261 tctttcagtt gactaacagg atacagttgg ctgtttttca cgttgttcac taacgaacga
     4321 gctaagatat ggctagattc ctgttctgca ctagctgcat acagaagtag ctcttctttt
     4381 tttattccat tgactggctg aatcgcctct acttccaata tccctttggt aagcgttcct
     4441 gttttatcaa aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt tccagttttt
     4501 acaacgatcc cgtttttact tgaacggctc attcctgcaa ctaaagcaat gggcgcagct
     4561 agaatcagtg gacaaggtga tgccactacc agtacctcag caaatcgtaa agggtctttt
     4621 gagaaccacc aagaaatacc accgatcaaa taagcaatca acgtgaaagg gactgcataa
     4681 cgatcagcta aacggacaaa tcgggcaggc ttttcttttg attcttcgac tagtttaata
     4741 atcatttgat actggctgtc tgcggcacgt ttttcagcga tcatttggat cgatccgtct
     4801 ccatttaaag accctgacat aatctgatct ccaggtttct tggtttccgg gcgggactca
     4861 cctgtcaaag aagattcatc gaccgaagat tctcctttgg tcactcgacc atctgcgggt
     4921 acaagttcgc caggcttgac tagtaactgg tcatcaatat ttatttcatc gattactata
     4981 tcctgcaatt ttccttcttt gaatcgatgg gcaattcttg gggtgttatc taacaaagac
     5041 tgcagttctt tacccgctcg tttattcgcg taatcctcta gactatctcc gcccgtaagc
     5101 atgatcaata tcatcaaact agcccagtat tctttgacgg ctaatgttgc aaggattgcg
     5161 gtaatcgcta atacatctac gccatatctt cctgaacgta acgtcttgat catttccaaa
     5221 aacatcaaga aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc aagggccggc
     5281 tggtgaaaaa caaattcact taataaagct atcccaccaa taagcaaagt aagtaaaaat
     5341 tttttgaaat gtgtcattgt tttttctcct cttttatgtg acttagagag gttagtaata
     5401 aatttccagc tgcctctttc atcttaagca tagtccttga ctagcacttt ttaaaacaat
     5461 aggccttaat tgaaaacaac tatcactatc agcgtttcca aaattcatca gttctacaaa
     5521 caaaaatatt cagcaaggat tactttttcg attcatcagt taaaaagtac cttttttcga
     5581 aaaactatgc tatactttta attcgttatt tacggcggaa ggagcaaaca tgcgatgaat
     5641 gagaaacaac tagaacaaac gcacgtggat gaaacgatcg aactaatcca gatcgaacaa
     5701 aatatgctgg atacaaaaca acaagaattg actcaggaaa tgaatcattc gacaaaagac
     5761 acagctaatc acaagattcg agctggttcc aatgaagctt tttatgaatc tgctgttgaa
     5821 tatcgccaac atgagcagga attgcttttg aaataccata ctcttgaagc acagcaaaaa
     5881 cgcctgcaaa cgttgtctgt catgaaagat agtccttatt ttgcccgtat cgactttaaa
     5941 gaagaccgtg aaacagaaac attatatcta ggtatcgctt ctttaagaga tctggcagaa
     6001 gaaacaatcg ttattgactg gcgcgcacca attgccaatc tttattatga aggtgagatt
     6061 ggtcccgcat tttatgaaac tgaaacagat agaatagatg tcgatctgca gttgaaacga
     6121 caatttaaaa tccaagaagg acgtatttta tctatggtag atacgtcgga agtaatcaat
     6181 gatgactttt tgctagagat attggatgaa tcatcaagca accatatgaa aaacatcgtc
     6241 tctacgatac aaaaagcaca aaatgcaatc attcgggaca caacaagtaa agtaatgctg
     6301 attgaaggaa tcgccggaag tggaaagaca tccgctttgc ttcaacgtat tgcttttctg
     6361 ttgtatcata atcgcaagtg gctcgaggct gaaaacgttc ttctattttc acccaaccat
     6421 ttgttttctg attacatctc gactgtattg ccttcattag gtgaaagcgg cgtgcctaca
     6481 cagacattca aaaattattt ggtgcagctg ctgccagagt tttctttact agatgaacag
     6541 caacaagaaa gtggtttttt gctaggggaa aaagatccga tacaagtgat gaaaagcgga
     6601 ttgaccttag tcgatcagat tgatcgctat atccaaggca tcacaaacta cggtcccttg
     6661 ttccgtgata tgaaaataaa tggccgtacg atcctatcaa aggaatcgat tcgccaatgg
     6721 tataaagaaa caaatgaaca attaccttta catcatagac tttctctatt acaaacgaag
     6781 ctcctgaaaa aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat
     6841 ctggcggaag aacagctcca agaactatac gcaacagatc caaatcttga atatacagaa
     6901 caaaaggagc gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt
     6961 cgaatccatc gagggatcat gaattatcaa tttgtcaata taacgaaaca atatctccac
     7021 tttctacaaa ctgtgccact ttctcttcta acacgacata aaatcagcga gaccaggtgg
     7081 caggaacatt tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat
     7141 gctgtactat tctttttact gatgcgccga acatatccag tgacggttac acacaaagct
     7201 cgctatatct ttattgatga aatgcaggat ttcgcaccat cacaagtggc attgctgcaa
     7261 gccttgttcc caaaagcaaa tttgactttc tgtggagatc taaatcaaaa tgtctttgga
     7321 aatgaaacga tcgttggatc attggaagac cttttcgtgg accgtgaagt cactcgtttc
     7381 caattaacga caagctatcg ctccacaaaa gaaatcactg attttgctaa tcattttatt
     7441 gtgaaagaac atcaagtaga aacaacagca agaaaaggag accttccttt gctcatccaa
     7501 ggaacaacag atgatatcaa attgaattgg ttagcagata ccattcgaac gactcaggga
     7561 aaagcccggt attggcgtac agcaatcata gctaaaacac aagaagaatg cgaagctctt
     7621 tatgctcaac tgccagacga gttgaaacaa caagtccagc tgattttaaa tgagagtgac
     7681 tttatgaagc gttccattat tttacttcca gcctttttag caaaaggtct ggagtttgac
     7741 cgagttttct tgtgggatgt agatgcagaa catttttctt ctcagcaaga caaattgatc
     7801 ctttacacca tgtgtacacg tgcgatgcat gaattgaccc tttttacctc caataatgaa
     7861 agtcctttgt taaacaatat ggatatttca tcttatgaga aaaaagtact gtaagtaaaa
     7921 agacaactgc tatcattaat gttagcagtt gtctttttat ttacaataat aattgcagaa
     7981 aaagtaattc aatcatttaa ggtcatttat tctatatata aatccaaatc tgtttcagga
     8041 tattttaatt tgacaagcga aataatcatt ttcagttctt ccatgttttc tggatattct
     8101 gggtcaaata ccttgctatg acggccaatc ccgccattgt cgatcaatcg gtaaattcga
     8161 ttccattcaa tgaattggtc aattgattgg aaattgctga ttggatagcc gccacaatcc
     8221 aaaatctctg atacacgaaa tgctaggtat gcactatcga tcactttatt tttttcgtgt
     8281 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
     8341 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
     8401 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
     8461 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
     8521 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
     8581 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
     8641 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
     8701 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
     8761 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
     8821 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
     8881 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
     8941 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
     9001 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
     9061 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
     9121 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
     9181 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
     9241 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
     9301 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
     9361 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
     9421 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
     9481 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
     9541 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
     9601 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
     9661 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
     9721 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
     9781 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
     9841 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
     9901 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
     9961 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    10021 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    10081 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    10141 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    10201 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    10261 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    10321 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    10381 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    10441 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    10501 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    10561 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    10621 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    10681 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    10741 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    10801 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    10861 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    10921 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    10981 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    11041 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    11101 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    11161 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    11221 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    11281 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    11341 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    11401 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    11461 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    11521 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    11581 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    11641 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    11701 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    11761 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    11821 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    11881 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    11941 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    12001 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    12061 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    12121 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    12181 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    12241 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    12301 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    12361 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    12421 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    12481 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    12541 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    12601 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    12661 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    12721 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    12781 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    12841 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    12901 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    12961 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    13021 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    13081 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    13141 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    13201 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    13261 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    13321 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    13381 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    13441 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    13501 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    13561 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    13621 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    13681 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    13741 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    13801 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    13861 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    13921 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    13981 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    14041 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    14101 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    14161 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    14221 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    14281 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    14341 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    14401 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    14461 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    14521 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    14581 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    14641 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata
    14701 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    14761 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    14821 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    14881 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    14941 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    15001 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    15061 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    15121 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    15181 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    15241 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    15301 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    15361 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    15421 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    15481 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    15541 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    15601 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    15661 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    15721 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    15781 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    15841 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    15901 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    15961 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca ataagcacct gctgtattga
    16021 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    16081 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    16141 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    16201 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    16261 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    16321 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    16381 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    16441 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    16501 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    16561 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    16621 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    16681 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    16741 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    16801 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    16861 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    16921 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    16981 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    17041 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    17101 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    17161 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    17221 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    17281 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    17341 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    17401 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    17461 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    17521 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    17581 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    17641 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    17701 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    17761 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    17821 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    17881 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    17941 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    18001 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    18061 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    18121 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    18181 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    18241 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    18301 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    18361 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    18421 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    18481 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    18541 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    18601 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    18661 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    18721 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    18781 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    18841 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    18901 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    18961 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    19021 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    19081 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    19141 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    19201 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    19261 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    19321 ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    19381 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    19441 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    19501 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    19561 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    19621 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    19681 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    19741 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    19801 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    19861 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    19921 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    19981 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    20041 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    20101 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    20161 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    20221 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    20281 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    20341 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    20401 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    20461 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    20521 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    20581 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    20641 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    20701 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    20761 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    20821 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    20881 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    20941 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    21001 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    21061 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    21121 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    21181 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    21241 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    21301 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    21361 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    21421 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    21481 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    21541 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    21601 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    21661 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    21721 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    21781 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    21841 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    21901 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    21961 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    22021 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca
    22081 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    22141 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    22201 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    22261 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    22321 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    22381 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    22441 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    22501 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    22561 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    22621 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    22681 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    22741 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    22801 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    22861 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    22921 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    22981 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    23041 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    23101 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    23161 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    23221 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    23281 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    23341 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    23401 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    23461 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    23521 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    23581 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    23641 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    23701 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    23761 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    23821 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    23881 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    23941 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    24001 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    24061 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    24121 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    24181 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    24241 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    24301 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    24361 acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    24421 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
    24481 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    24541 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
    24601 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    24661 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    24721 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    24781 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    24841 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    24901 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    24961 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
    25021 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
    25081 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    25141 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    25201 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    25261 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
    25321 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    25381 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    25441 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    25501 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    25561 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    25621 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    25681 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    25741 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    25801 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    25861 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    25921 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    25981 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
    26041 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    26101 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    26161 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    26221 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    26281 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    26341 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    26401 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    26461 cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc
    26521 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
    26581 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
    26641 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
    26701 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
    26761 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
    26821 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
    26881 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
    26941 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
    27001 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
    27061 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
    27121 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag
    27181 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
    27241 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
    27301 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
    27361 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
    27421 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
    27481 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
    27541 agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg
    27601 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
    27661 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
    27721 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
    27781 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
    27841 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
    27901 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
    27961 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
    28021 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
    28081 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
    28141 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
    28201 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
    28261 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
    28321 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
    28381 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
    28441 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
    28501 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
    28561 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
    28621 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
    28681 acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag
    28741 cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg
    28801 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
    28861 gctggttcga tctgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
    28921 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
    28981 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
    29041 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
    29101 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
    29161 acatagccta caaactgtca ctcaaactta agccctattt ttgtctattc ttactgacaa
    29221 aaacgttttc ttaaaaaaga acaaaaaaac tggcgtagct cttttacaag ccacaccagc
    29281 ttttgatacg ttgtttattt tacatattca tgaacaagtt caacgacttc ttccatcata
    29341 tcgcattctt tcaatgcacg atcagcaagt tcagccattt tcgctgtgtc caagcgtttc
    29401 attaaactac gcgttttaag aatagatgtc gcactcattg agaactcatc tagacccata
    29461 cctactagca atggaacagc tgtttgatct ccagccattt ctccacacat acctgcccat
    29521 tttccttcag cgtgtgctgc atcaattaca tttttgatca aacgtaaaat tgatgggtta
    29581 tatggttggt acaagtaaga aacgcgttcg ttcatacggt ctgctgccat tgtgtattga
    29641 atcaaatcgt tcgtacctac agagaagaaa tctacttctt ttgcaaactt gtctgctaag
    29701 actgctgctg ctggaatctc gatcatgatg ccgacttgga tcgtatcaga tacttccttg
    29761 ccttcactta tcaatttttg tttttcttct tcaaagatcg cttttgctgc gcggaattct
    29821 ttgagtgtag ctaccatagg gaacatgata cgtaaattac catgaacaga tgcacgaagc
    29881 aatgcacgca tttgtgtacg gaacatttcg tcgccttgtt cagataaact gatacgcaat
    29941 gcacgatatc ctaagaatgg attcatttca tgaggtaatt gcagatatgg tagttcttta
    30001 tctccaccga tgtccattgt acgaacaact acaggttttc cttccatacc ttctaataca
    30061 gctttgtaag cttcatattg gtcatcttct gttgggaagt ctggagaatc catatacagg
    30121 aattcagtac ggtacagacc aactgcttct ccaccattat tatggacacc aactagatct
    30181 tttggtgttc cgatgtttgc agctaattca aaatgcttgc cgtcagctgt gacagtttct
    30241 gcatttttta atttttccca ttctgctttt tgtgctgcgt aatctgcacc tgctttttca
    30301 aattcagctt tttgttcatc tgtaggatcg atgattacat cgccttcgat cccgttgaca
    30361 gccaaaatat cgccttcttt gactttagca gtaatttctt ttgtcccaac gattgctgga
    30421 atttctaaag aacgtgccat gatagcagaa tgtgaagtac gtccaccaat gtctgttaca
    30481 aacgctttta caaaattgcg atctaattgt gctgtatcac ttggagtcaa gtcatgtgca
    30541 acaacgacca cttcttcgtt gatcattgat ggatttggta aagtaacgcc taataagtgc
    30601 gctaaaatac gtttagcaac gtcacgaata tctgcagcac gctcttgcat ataagcatta
    30661 tcttccattg cttcgaacat accgatatac atatccgtta cttcttttag cgctgattca
    30721 gcatttacgc tgttgtcttt aatattttgc ttgatttgac ctaccatttc tggatctgaa
    30781 agaaccatca aatgggcatc aaatacttgc gcttctgctt cacctaagct ttgcgctgct
    30841 ttttcacgaa tttgctgcaa ttcttcagta gatttagcta atgcagcatc caaacgagct
    30901 tcttcagccg cagtatcttc tactgttgcc ttactgaatg ttaaatccgg ttgaactagc
    30961 aggtaagctt ttgcaacggc tacaccgtca ctagcggcaa tcccttttag catttcaacc
    31021 attattcaga taagccttct ttcttcattg tttcaacgat tgctgccatt gcatcagctt
    31081 cgtcagcacc ttctgcagta atagtaacat cagaaccttg acctacacct aaagacataa
    31141 cgcccatgat tgatttcaag tttactgatt tacctttata ctctaagttt acatcagagt
    31201 taaatttgct tgctgtttgt actaatagtg tagctggacg agcgtggatc ccagtttctg
    31261 ctactacgtg aaattctttc ttttccatat taatcggtct cctttaagtg agtgttgttt
    31321 ttatgtgagg gttttctttt ttagtaattc agagaatcct aacaaatgcc accctttcat
    31381 ctgtaaagat taacattttt tttcgttaga tacaacagtt ttcattcaat ttcagataaa
    31441 agtattcaaa ttattccgga ttctttttca tccttttctt cacattttat aacaaacgtg
    31501 tttatttcct ttttcttgtc gctttctctt ctcctgacgt ttatcattct ttcctttgac
    31561 cctctttgtt ttcatatgaa acgaattact tgcttccctt tttattttta gtataataag
    31621 agtcaaaggt caggaatggt caatagagat ttcacctttt ctacctcata aatctcttaa
    31681 aaaactaggg gttattcttt tacctcatga aaaacgagat gtaaaatatg gatgtaccca
    31741 tttttgtgaa aggacgtgaa tgattcatgc tttgtcaaaa ctgcggcaag aatgaggcaa
    31801 cgattcactt gtatgccact gtcaatggtc agcggacaca actcgattac tgtcagagct
    31861 gttatcaaaa aatcaaaaat caacaaaatg gaggtctgac taatatggcc caaaatgatc
    31921 catttggatt tggcagtttg gacgatcttt tccgttccat gtctcgccaa atgcaacagc
    31981 aaggaaatta tgagcaaaca ccaccaacac aatttggcgg aaacaacaac ttcaatggtg
    32041 gtcagccacc tcaaggcaat acagaaggtt tattgggaga atacggaatc aatatcacag
    32101 aacaagcacg acaaggtgat atcgatcctg tgatcggacg cgatgacgaa atcaaacgag
    32161 tcattgaaat tttaaatcgc cgaaccaaga acaatcctgt cttgattgga gaaccaggtg
    32221 ttggtaaaac agcagttgtt gaaggtttag cacaaaaaat cgtagatggt gatgtaccgc
    32281 aaaaattaat ggacaaagaa gtcattcgtt tagatgtcgt atctttagtc caaggtactg
    32341 gtatacgagg acaatttgaa gaacgtatgc aaaaattgat tgaagagatc cgtcaagcag
    32401 aaaatgtgat cctattcata gatgaagttc acgaaatcgt cggtgctggt tctgctggtg
    32461 atggaaatat ggatgctggt aatattttaa aaccagcttt agctcgagga gaattgcaaa
    32521 tggttggtgc tactacctta aatgagtacc gcatcatcga aaaagacgct gctttagaac
    32581 gtcgtatgca acctgtacgg gtagatgaac caactgtcga agaaacgatc agtattttga
    32641 aaggtttgca aaaacgttat gaagactatc atcatgtaaa atatactgat gaggccatcg
    32701 aagcagccgc aactttatcc aatcgttacg tccaagatcg cttcttgccg gataaagcca
    32761 tcgatttgct agatgaaaca ggttctaaga aaaacttgac gatccaaatc gttgatccta
    32821 aaacaatcga gaaaaaactg caagaagcag aagaacaaaa agttttagct tctcgagaag
    32881 aagatttcga aaaagcagct tattatcgcg atcagatcaa taaacttcaa aaaatgaaag
    32941 aacgtcagct cactgaagag gaaacacctg tgattaccga aaaagatatg gaaaaaatcg
    33001 ttgaacaacg gacaggtatt ccagtgggtg aattaaaaga aaaagaacag acacaactga
    33061 aaaacttagc agatgacttg aaagctcatg tcatcggaca agataacgct gtagatcgag
    33121 tagcgaaagc gattcgccga aaccgtgttg gattaaacaa acagaatcgt ccaatcggct
    33181 ccttcttatt tgttggacca actggtgtag gtaaaacaga gcttgcgaaa caacttgcct
    33241 acgaactatt cggttcacaa gattcaatga ttcgtttcga tatgtctgaa tatatggaaa
    33301 aacatagtgt ctctaaattg attggttctc ctccaggtta tgtcggatac gaagaagctg
    33361 gtcaattaac agaaaaagta cggagaaatc cttacagtct tgtcttgtta gatgaagtag
    33421 aaaaagctca tccagatgtt ttgcatatgt tcttgcaaat cttagatgat ggtcgattga
    33481 cagatgcaca aggaagaacg gtcagcttta aagatacgat catcattatg acaagtaatg
    33541 ccggaactgg taaagtagaa gcaaacgttg gttttggtgc ggcacgcgaa ggtgttactc
    33601 gttctgtatt gaaccaatta aacaactact ttacaccaga atttctgaac cgttttgacg
    33661 ggatcatcga attcagtgca ttatcaaaag aaaacttgat gacaatcgtc acactaatgc
    33721 tagacgatgt caaccaaatg ctggcagcac agcaacttca catcgaagtt cctacgaatg
    33781 tcaaagaaaa attagttgac ttaggctatg atccatctat gggtgctcgt cctcttcgac
    33841 gcacgatcca agaacaaatc gaagatggta tcgctgaatt ttatcttgat cacccttcta
    33901 tccatgagtt gaaagcaaaa ctggataaag atggaaaaat catcgttact tctaaaccag
    33961 aacgtttggc taaagaatca gccgaagaaa cggctgaata aatccagttt tatagcgtga
    34021 gacggtattt ctttgagaaa tgccgtctca ttcttttttc agaaaagtca aaatactgtt
    34081 cattttataa aaaagaaatt ataataagag aaacaagcaa ttaaaaatac ataaaagagg
    34141 tgagcgccat gaaattggta aatgtaacaa acagccattc ccgtcttgta ttaaaccagc
    34201 ttgaaaacac tgatgcccat ctagtcaagg tctacacagc cggaaatacc actattgtct
    34261 acactgaagc ccctgaacat aatgaaattc tgctgatcaa tgacaaacgt aaaatccagc
    34321 cgaaagaaat cgaagatgct aaaaattatt tcttagataa aatcaaagac gccgtttacc
    34381 acgaggatga aatcaaagta atcgaattgg caggccttgt tgaaatctcg attccaaaac
    34441 atgaattagg cgctacatta gccgtgtaaa caaattttga acataataat taaagagcgt
    34501 gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca
    34561 gaagcaactc cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga
    34621 attccagctt atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt
    34681 tttgattttt ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct
    34741 ttatttttaa ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg
    34801 tttttgtccc agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc
    34861 tgtttttttc tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta
    34921 atcatatgta cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat
    34981 atagtaaaaa gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg
    35041 tttttgtttg gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca
    35101 tcaaactttt taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt
    35161 tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac
    35221 tcatcttctc atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag
    35281 acatgattac ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta
    35341 gctgaccaac agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg
    35401 ctccttcttg aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt
    35461 ttgcagtcac tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt
    35521 ttagttttcc ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga
    35581 tgatatcgcc tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga
    35641 cattgcttaa tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga
    35701 atgttccaga acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg
    35761 cttggatctt gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat
    35821 cttgtgtttt gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct
    35881 gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa
    35941 ttttttcttg attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt
    36001 gttcatataa ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca
    36061 attctactaa tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt
    36121 agatatcata aagaccttct agccctttac ccataccgat tggccaattc atcggataag
    36181 attcgatatc taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac
    36241 gatctagttt gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata
    36301 atttctttgt ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca
    36361 cggccatcaa agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga
    36421 tatttactcg tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac
    36481 gttgtttctc gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta
    36541 cggtcccggc ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt
    36601 tcccagcatc cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta
    36661 attttggatt attcataacc taactccatt tcttccattc aattatttat attttctcta
    36721 ggcacagatt gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc
    36781 gtgtagcagt ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc
    36841 ccacccaagc caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca
    36901 ggatagcttt ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca
    36961 gcgaatgaaa aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag
    37021 aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat
    37081 cttcttggct cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc
    37141 gcttctgttt cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc
    37201 atttcttcag aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc
    37261 ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc
    37321 agatcactgt caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat
    37381 tcgtgttcat cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct
    37441 ccgacgattt cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca
    37501 tctaataaaa tagccatctg attctgcgtc tttttcattt catataacag atcatcaata
    37561 aagaccgttt caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca
    37621 aagccgaatt tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta
    37681 tctttgtctt cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca
    37741 ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact
    37801 tctcgggcaa ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta
    37861 ttcaatacgc cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct
    37921 tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc
    37981 cagacgaagg gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg
    38041 acttcttctg atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat
    38101 acataggtca aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca
    38161 cctaataccg gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg
    38221 ttgaccaaag tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt
    38281 tttaaaagct tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta
    38341 ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg
    38401 atcaataata aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca
    38461 ttctctcctt ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca
    38521 cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac
    38581 ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt
    38641 gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc
    38701 aagcaatccg gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat
    38761 tttcagtgat ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat
    38821 tagcacaacc acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa
    38881 tgcaggtgct agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat
    38941 gaccccaaat aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt
    39001 accaacgaat aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag
    39061 ttgccccaat aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt
    39121 tggcactagt ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat
    39181 tactacggtt gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact
    39241 aaggaattgc tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat
    39301 atctacttcc ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt
    39361 gatgaaatca ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac
    39421 actaagtaaa atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca
    39481 tattcttttg atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag
    39541 aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa
    39601 aataaagttt atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga
    39661 ccaaaacatt agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct
    39721 tactagtata tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt
    39781 gccatacttt ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc
    39841 atgaaagttg tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc
    39901 aggcatcaag tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat
    39961 gaagcccatt gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta
    40021 cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa
    40081 tacagacacc aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat
    40141 caaggctata atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa
    40201 ctaggatata cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg
    40261 aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa
    40321 atctctcact ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg
    40381 ttataccaaa tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca
    40441 accagaatct tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt
    40501 ttgctgtgca tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc
    40561 ttgatatcca agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa
    40621 gctagattac aataatagga aacccccttt tctctgatca ttgctattac attgaaccag
    40681 tattttttat gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat
    40741 tcagtgacta atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc
    40801 atggtatctc gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca
    40861 aactctcctt cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg
    40921 tgtttgggtg ttcattacac cgacatcata attattatat caataaaaat gagctttgcc
    40981 tagaaaggat tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc
    41041 ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg
    41101 gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc
    41161 agatgactaa aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc
    41221 tgcttttcaa atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt
    41281 tctggctttg tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc
    41341 attggaaatg tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac
    41401 gctccagaat gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt
    41461 tttgttttta caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg
    41521 atcggacaag tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc
    41581 attgcttggt tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca
    41641 aagattttcc tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact
    41701 actcgcatca cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc
    41761 gcgcctgtcg tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc
    41821 atttccccat caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt
    41881 gagtaataac caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc
    41941 tcttcaattg taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact
    42001 ctggtttgct gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc
    42061 caaggcaggt ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt
    42121 gtttctttat ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat
    42181 gaatcgctgg atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag
    42241 atttcttttt ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac
    42301 taaataatca tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg
    42361 gatcacttct tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc
    42421 tttttggctt tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg
    42481 aacttggtct tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc
    42541 tttttgctgc atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt
    42601 gcgcacataa ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc
    42661 tacgatttta tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc
    42721 tttttccgat cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc
    42781 tccgctactc ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa
    42841 tcgatagcgt actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac
    42901 gcgatagaaa gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct
    42961 cgaacatata ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga
    43021 ataaaccaca gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg
    43081 acaaacggtt cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt
    43141 caaacggttg atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat
    43201 cacgaaagca actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa
    43261 agatcgcgta gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca
    43321 cttagcttat caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa
    43381 attcaaacca gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc
    43441 ttggcattat agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat
    43501 tgaagctggt ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt
    43561 tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga
    43621 tgcacccatc tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat
    43681 cggtgttaaa actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca
    43741 aaaaaacgca atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca
    43801 aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg
    43861 aaaagaagcc attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt
    43921 ctttcaactc aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga
    43981 tgtccagcct aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag
    44041 tgtagcaaaa caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga
    44101 tgcaaaaaaa aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc
    44161 agagcaagtt ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga
    44221 tccaccacgt acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa
    44281 aaaacttgtt tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag
    44341 taaaaagttc aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg
    44401 tgaagttgtc gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc
    44461 tgccctgtat caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata
    44521 ctgtacttgt gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga
    44581 catagttttt gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata
    44641 aaatgcattt gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg
    44701 atcctgcgga agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc
    44761 tatttttgtg atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg
    44821 tttggtaatg tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat
    44881 atttaatgtt cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa
    44941 aggctcaaag ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt
    45001 agcttcgata ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc
    45061 tgctgatgtt cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc
    45121 tggaatgcta aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat
    45181 ctttttcatg tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta
    45241 acacaaacat tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat
    45301 gcagattata acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat
    45361 atgatctttt tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa
    45421 ttatcaattt ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt
    45481 aaactaatat tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg
    45541 gtttgtgtta taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc
    45601 ggaaaatatt actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg
    45661 ccgatgtgga ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac
    45721 aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat
    45781 tttatatggt ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt
    45841 acacttttag ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg
    45901 gcaatgtggc tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat
    45961 tcaatttatt ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa
    46021 aagtgccacc taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact
    46081 atttttttct agtacccact tgttgttcca tactcattat tctggtcata accataacca
    46141 ttttgttcct gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac
    46201 tgacttgtat cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg
    46261 ttttctgaat aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc
    46321 gaatcgccat cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta
    46381 ggtaatgtat cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga
    46441 agatcatttt ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc
    46501 aactggtctt gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg
    46561 tcatcccaat ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa
    46621 ttgcttacgc catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga
    46681 cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa
    46741 cctttttctc cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag
    46801 tcaattttat tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc
    46861 aaattgatcg ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg
    46921 gccatttcga caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct
    46981 acgatattcg tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga
    47041 ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg
    47101 gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca
    47161 gaacgtttgg aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct
    47221 tctcccataa atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct
    47281 aacaaggtta atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct
    47341 aaaat
//
LOCUS       DAZAID010000012        44828 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000012 DAZAID010000000
VERSION     DAZAID010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.012"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..1012)
                     /locus_tag="ACG9BE_000611"
     CDS             complement(2..1012)
                     /locus_tag="ACG9BE_000611"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN3031390.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1192..1536)
                     /locus_tag="ACG9BE_000612"
     CDS             complement(1192..1536)
                     /locus_tag="ACG9BE_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFN3031391.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1529..1963)
                     /locus_tag="ACG9BE_000613"
     CDS             complement(1529..1963)
                     /locus_tag="ACG9BE_000613"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFN3031392.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2101..2838
                     /locus_tag="ACG9BE_000614"
     CDS             2101..2838
                     /locus_tag="ACG9BE_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3031393.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2831..4042
                     /locus_tag="ACG9BE_000615"
     CDS             2831..4042
                     /locus_tag="ACG9BE_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3031394.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4246..5028
                     /locus_tag="ACG9BE_000616"
     CDS             4246..5028
                     /locus_tag="ACG9BE_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFN3031395.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5098..5760
                     /gene="trmB"
                     /locus_tag="ACG9BE_000617"
     CDS             5098..5760
                     /gene="trmB"
                     /locus_tag="ACG9BE_000617"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFN3031396.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5858..7612)
                     /gene="ade"
                     /locus_tag="ACG9BE_000618"
     CDS             complement(5858..7612)
                     /gene="ade"
                     /locus_tag="ACG9BE_000618"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFN3031397.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7744..8673
                     /gene="comGA"
                     /locus_tag="ACG9BE_000619"
     CDS             7744..8673
                     /gene="comGA"
                     /locus_tag="ACG9BE_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFN3031398.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8630..9679
                     /gene="comGB"
                     /locus_tag="ACG9BE_000620"
     CDS             8630..9679
                     /gene="comGB"
                     /locus_tag="ACG9BE_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFN3031399.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9676..9954
                     /gene="comGC"
                     /locus_tag="ACG9BE_000621"
     CDS             9676..9954
                     /gene="comGC"
                     /locus_tag="ACG9BE_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFN3031400.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9929..10399
                     /gene="comGD"
                     /locus_tag="ACG9BE_000622"
     CDS             9929..10399
                     /gene="comGD"
                     /locus_tag="ACG9BE_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFN3031401.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10371..10718
                     /locus_tag="ACG9BE_000623"
     CDS             10371..10718
                     /locus_tag="ACG9BE_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031402.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            10681..11106
                     /gene="comGF"
                     /locus_tag="ACG9BE_000624"
     CDS             10681..11106
                     /gene="comGF"
                     /locus_tag="ACG9BE_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFN3031403.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            11078..11437
                     /gene="comGG"
                     /locus_tag="ACG9BE_000625"
     CDS             11078..11437
                     /gene="comGG"
                     /locus_tag="ACG9BE_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFN3031404.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11609..12616
                     /locus_tag="ACG9BE_000626"
     CDS             11609..12616
                     /locus_tag="ACG9BE_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3031405.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12641..13825
                     /locus_tag="ACG9BE_000627"
     CDS             12641..13825
                     /locus_tag="ACG9BE_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFN3031406.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14262..15845)
                     /gene="abc-f"
                     /locus_tag="ACG9BE_000628"
     CDS             complement(14262..15845)
                     /gene="abc-f"
                     /locus_tag="ACG9BE_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFN3031407.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15950..16078)
                     /locus_tag="ACG9BE_000629"
     CDS             complement(15950..16078)
                     /locus_tag="ACG9BE_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031408.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(16423..16566)
                     /locus_tag="ACG9BE_000630"
     CDS             complement(16423..16566)
                     /locus_tag="ACG9BE_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFN3031409.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16789..17268)
                     /locus_tag="ACG9BE_000631"
     CDS             complement(16789..17268)
                     /locus_tag="ACG9BE_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN3031410.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17341..17625)
                     /locus_tag="ACG9BE_000632"
     CDS             complement(17341..17625)
                     /locus_tag="ACG9BE_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031411.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17703..17897)
                     /gene="ssrS"
                     /locus_tag="ACG9BE_000633"
     ncRNA           complement(17703..17897)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG9BE_000633"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17969..19255)
                     /locus_tag="ACG9BE_000634"
     CDS             complement(17969..19255)
                     /locus_tag="ACG9BE_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFN3031412.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19393..20064
                     /locus_tag="ACG9BE_000635"
     CDS             19393..20064
                     /locus_tag="ACG9BE_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFN3031413.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20030..20518
                     /locus_tag="ACG9BE_000636"
     CDS             20030..20518
                     /locus_tag="ACG9BE_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFN3031414.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20531..21478
                     /gene="prmA"
                     /locus_tag="ACG9BE_000637"
     CDS             20531..21478
                     /gene="prmA"
                     /locus_tag="ACG9BE_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFN3031415.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21479..22243
                     /locus_tag="ACG9BE_000638"
     CDS             21479..22243
                     /locus_tag="ACG9BE_000638"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFN3031416.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22476..24689
                     /locus_tag="ACG9BE_000639"
     CDS             22476..24689
                     /locus_tag="ACG9BE_000639"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFN3031417.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24700..25146
                     /gene="dtd"
                     /locus_tag="ACG9BE_000640"
     CDS             24700..25146
                     /gene="dtd"
                     /locus_tag="ACG9BE_000640"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFN3031418.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25317..26576
                     /locus_tag="ACG9BE_000641"
     CDS             25317..26576
                     /locus_tag="ACG9BE_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFN3031419.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26665..27612)
                     /locus_tag="ACG9BE_000642"
     CDS             complement(26665..27612)
                     /locus_tag="ACG9BE_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN3031420.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28006..29298
                     /gene="hisS"
                     /locus_tag="ACG9BE_000643"
     CDS             28006..29298
                     /gene="hisS"
                     /locus_tag="ACG9BE_000643"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFN3031421.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29313..31082
                     /gene="aspS"
                     /locus_tag="ACG9BE_000644"
     CDS             29313..31082
                     /gene="aspS"
                     /locus_tag="ACG9BE_000644"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFN3031422.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31191..32066
                     /locus_tag="ACG9BE_000645"
     CDS             31191..32066
                     /locus_tag="ACG9BE_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN3031423.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32332..34215
                     /locus_tag="ACG9BE_000646"
     CDS             32332..34215
                     /locus_tag="ACG9BE_000646"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN3031424.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34290..34574)
                     /locus_tag="ACG9BE_000647"
     CDS             complement(34290..34574)
                     /locus_tag="ACG9BE_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN3031425.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34564..35298)
                     /locus_tag="ACG9BE_000648"
     CDS             complement(34564..35298)
                     /locus_tag="ACG9BE_000648"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFN3031426.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35427..36050
                     /locus_tag="ACG9BE_000649"
     CDS             35427..36050
                     /locus_tag="ACG9BE_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFN3031427.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36242..37363
                     /locus_tag="ACG9BE_000650"
     CDS             36242..37363
                     /locus_tag="ACG9BE_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFN3031428.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37370..40498
                     /locus_tag="ACG9BE_000651"
     CDS             37370..40498
                     /locus_tag="ACG9BE_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3031429.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40517..43711
                     /locus_tag="ACG9BE_000652"
     CDS             40517..43711
                     /locus_tag="ACG9BE_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFN3031430.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43857..44564)
                     /locus_tag="ACG9BE_000653"
     CDS             complement(43857..44564)
                     /locus_tag="ACG9BE_000653"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3031431.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14384 a         7961 c         9405 g        13078 t
ORIGIN      
        1 tttattttga tgaatcagtt gtttcttcag ttgaagattc ttttgttgaa tcagttgatt
       61 ttgtgtcaga actctttgtt gaagctgtag tttcacttga gtctttcgta cttgatgaag
      121 ttgttgtgaa tgcactcaac acattttcaa aggcatcatc tttgattttt acatttgcat
      181 cttttaattc ttcaccgatt actttagttg tgaatgcatt gtcacttagt ttagtttcag
      241 tagcgatatc ttttagttga tctttgtatt tatccatgtc attgccttta ttttggtttt
      301 tcaccatttt gacaacgtag tattctgtcg cataagaagt aggatttgtt gtagtaataa
      361 catcggagat ctcaccgtct ttcaatttga aagctgcttc ttttacttct gcaggaatag
      421 ttgttgtcgt tgagtcgaat ttaactttac cgccatcttc ttttgtctct gtatcagttg
      481 atttgtcttt ggctaattta gagaaatcat ctccatcatc cgctgatttt ttcacatctt
      541 ttgcttcatc ttcacttgat aatttgatga tttgtgcttc tacttctgga tggaaagatt
      601 tccaagcagt tttcaaatcg tcatctgtaa tatctacgtg agctttcaag ccagcttcaa
      661 aagctaaatt gttacgaata tattctttat aagtatcttt cgtatatcca gcagcttcta
      721 attgtgattc aaaagtatca cctaatgatt tcgcttgttt gtcgtattct gcatccactt
      781 gcttatcggt tactttatcg ccatatttgt tattgaatac tttataaatg atcatccgtt
      841 gtactaagga ttggttcgat gattcaagtt ttgcttcatc atagaaatca gatacagtga
      901 tcgttccgcc tttcatcgtt gcgatatctt tattagtatc gcctgaacaa gctgctagag
      961 ttaaaacagc caacgcacta gtagctgcca aaataattga tttttttttc attaaaattt
     1021 gcactcctgt aattagttat tatttttata tattaactat accatatccc atttccttta
     1081 aaaacgttgt cgtggtagtt tcaaacaaaa ttcaaaattt catcacattc ctaggaaata
     1141 cgcaaacttt tctcacttat atagttaaat ctgttgagaa aataatcatt attatatttg
     1201 ttcaggatac tctggcagat cagcttttag tttttctact tgttcattga tttgtgtgat
     1261 acgtggttcg gcttggaatt taaaatcttc gatatctttt ttgattgctt cttggaaagc
     1321 aggaatcaca gattcagctg tttcttttgt ttcgatcaat gcgcttttaa aatgattcag
     1381 gctgttattt aaatcatttg tcagagttgt tgcttcttcc aattcttcga tcagtttctt
     1441 tcttgtttca ttcccgctcc tgggcgccaa taacagacct ccgacagtgc cagcagcagc
     1501 tccaaataac agtcctttga aaaagttttt aatcatcgca ggacacctgc tttacgattg
     1561 tttcttgaat agcttttagt tcttccgatc cataactatc ttgatgattg acaaagtgaa
     1621 tagaaaaatc atcctctttg ctgtagcgag ggactaaatg gatatgtgaa tgaaaaacag
     1681 attgataggc tagttcacgg ttgttattca agatattaag acctttcatt tctggaaaag
     1741 ctttttccaa tgcttgagca atcttaggca cacgcgaaaa aacctctcct gcaagttcgg
     1801 gatcatattc aaaaatatct gctacatgac gtttaggcac aactaatgtg tgtcctttcg
     1861 ttgcttgaga gatgtctaaa aaagcataga ccttgtcgtc ttcatatatt ttatagcttg
     1921 ggatttcttg attgatgatt ttgcaaaaaa tacagttttc catataaaac atcctttcta
     1981 taaagaataa atacttcagc ttaactttac cataagttaa agctattgta aggaaaaaaa
     2041 caaaataccg tcattttctt cgtggtataa taaataaaaa taaatggcgg aggaaaaaga
     2101 atgagtttaa ttatcgatca tgtcacaggg gggtacggac atctccctgt gttaaaagat
     2161 atcagtttcg atgtacagcc tggagagata gtagggttga ttggactgaa cggagcaggg
     2221 aaaagtacga ccatcaagca cattatcggt ctgctggaag cttcaaaagg aacgatagaa
     2281 atcaatcatc tatccttaaa gaaaaatacg gaagaatatc gcaaacaaat tgggtatatt
     2341 ccagagactc cttctttata tgaagaattg acattgagag aacatatcga agtcactgcg
     2401 atggcctacg atatacctat ggatatagca atgaaaagag cagaaaagtt gttggagact
     2461 tttcggttat ctaatcgtct tgactggttc ccagctaatt tttcaaaggg aatgaaacag
     2521 aaagtgatga tcttatgcgc ctttttgatc gagccttctt tatatatcat cgacgaacca
     2581 tttttaggct tggatccact agctatcaat gcattgcttg aattgatggt agaaatgaaa
     2641 aaagaaggag ccggtatttt gatgtctacg catattttag ctactgcaga aaaatattgt
     2701 gatcgttttg tggtcttaca taacggagag atacgagcag acggttctct tgagatgttg
     2761 agaaaagaat ttcagctgcc ggattcttct ttggacgaaa tctatattgc tttaacaaag
     2821 gagcaggcac atgagtagtt tttttcaaaa acgtttagca agacatcaaa agaaaatgat
     2881 gaaatatatg cgttatgtat tgaacgatca ttttgtttta gtttgtcttt tcttgattgg
     2941 cggtgtggga ttatattatt ctaattggtt gaaaaccctg cagccaccgt ttgcaattgg
     3001 agggctcata atctctgtat tttggatgct ctgtttattc gtgggtaaga tggctacttt
     3061 tgcagaggca gcagatattg tttttttact tcctaaagaa aaagaaatgc aaacctattt
     3121 ggaacgagct tttcgctatt cttgcatttt tccgttgatc tttttgatat tggcatgcgg
     3181 attggccatg ccattagtgg tggttagtac ggagcaatcc ttttcgatgt ttttcgtcta
     3241 tctgatcata ttatggagtt tgaaattcag tcatctgcaa atcaagagag cagaattgtt
     3301 tcgactagat caacaagtta ttcgttattg gaaattaggc tggttttttt cttctctgat
     3361 ctttttgttt ctatcatttt attggctaat atgggcagga atagcaggtg cattagtaca
     3421 ggcaagtcta tattactggt ttttgtggaa gcaacaagaa aaaggattgg attgggagaa
     3481 aatggtgcat gtagaagaac ataggttgca tcagatctat caatttatca atttattcac
     3541 agatgttcct gaaattacgg caaagatcaa aagaagacgc tatttggatc ctatattgac
     3601 tcatattaaa agagaatcaa aaaacactta tctttacttg tttgcaagaa gattggcaag
     3661 aggttctgat tttagtggtc tttatttccg attagtaatt ataggtgggt tattgattgc
     3721 cggagtagtc gacttttatt ttagtctgat tatcggtttc ttatttttat atttgatcgg
     3781 tttccaactg ttacctttag cgaaccagtt ccgctatatg gttctcgttc agctttatcc
     3841 aatccataag aatcagaaga agcaggcgat tcagtatcta ctcacatggg tattaattat
     3901 cgcgtcggtt atttttggtt tgattgctgc agtcgtatta gatgggcagc agcgatgggt
     3961 tccgctagcg gcttatttga ttgttactat gctctttgtt aaactatatg taccaactcg
     4021 tttgaagaag atgtcagact gaaaacaaag ctgtgtaggg taaaaatgag aatgaaaaat
     4081 agagcgttga cagtaagaat gctgttcgtt ctattttttt tgagaaaagc tggttttttg
     4141 aatggcaatt aaatgaaaaa gaacgtatat gtgcttgacg tgtagtgttc aggcgactag
     4201 aataataagt actgcaaaac aaagagtaag aggacgggag aatgcatgat ggagtttcag
     4261 ttagaccaag agtggcggtt acgtcccatc aaaggcgaca caggaaaaac atacataggc
     4321 atgcgtgatg aggacaaagt ctttattaaa cgaaacacga ctccgatgtt ggctgctctt
     4381 tcaagggaag gaattacacc aaaacttgtt tggacaaaac gaacggggaa tggcgatact
     4441 ttgacagcac aagagtggct ggaaggtcgc ttgctgactc ctaatgagat tggtcaaaga
     4501 aacgacgtga ttgatgtttt gtaccattta catcattcaa attctcttaa aaccatgctg
     4561 acccgtatcg gtggtcgtgt catgacgccg gagaagatgc tgcaaaaata tgaaaaagaa
     4621 ttaccggaag aactccagaa aaatcaattt ctgcaaattg tccatcgttt tcttcgtgag
     4681 cagatcccgt caatagataa ggaaaactta tcagtagtac acggagatgt aaaccatcgt
     4741 aattggttag tatgtaaaaa ttatttatat ctagtcgatt gggattcaat catgtttgca
     4801 gaccctgcgg tggatattgc aacgatttta ggaaattatg tgccactgtc tagctggaat
     4861 cagtggctga tcagttatgg catacgcccg actgacgaaa tgcttgaaaa atttcattgg
     4921 tatgcggcca tgaatttgct ccaagaaatc accagatatt gcaaacgtga agatcatcga
     4981 cggatgaacg aagaaatttt acaattaaaa cgaattttca gtggctgagg tttggcccaa
     5041 actctgcgga gatttggcca gaccttttta attagaggaa aggaatggag ataaataatg
     5101 cgtgtccgaa atcgtccggg agcagcggaa atgttagcgg ctcacccgaa ttttgtaatt
     5161 agtgatccaa cattgtggaa gggaaaatgg aatgaattgt ttgaaaacga tcatccaatc
     5221 cacattgaga ttggtatggg gaaaggccag ttcatcactg gaatggcaaa agcccatcct
     5281 gaaatcaatt atattggagt agaaatgcag gtcagtgttg tttcgattgc actagacaag
     5341 ttgatcgaac aaccgcttcc taacctgaaa cttctacatg tggatggatc agcgttgaca
     5401 gagtatttcg ctgacagcga agtagatcaa atctatctga atttttccga tccatggcca
     5461 aaaaaacgtc atgaaaaacg gcgattgaca tataagactt ttcttgctgt agatgaacag
     5521 attcttcgtc caaatggaga aatccatttc aaaacagata atcaaggatt gtttgagtat
     5581 tctctggcaa gtttttctca atatggcatg atcctcaaac aggtatggtt agatttacat
     5641 caaagtcaat ttgaaggcaa tattatgacg gagtatgaag aaaaattttc ttcaaaaggt
     5701 caacgtattt atcgagtaga agctcaattt caagataaga ccaaaaagaa aagtctgtga
     5761 aactaacttt ctgctttcat aaaaacatta atatccataa ataaatccca gaaaatgaac
     5821 attacagatg taatgtattt tctgggattt atttgattta aaatgtattt atttcatcgg
     5881 tttttccaca aggtggaatc aattggaagc gagaaaatga aaataatcct ttgtcggtga
     5941 tcttcaattc gggtatgaca gataatgtta agaaagaaag tgtcaaaaaa ggatcaaagg
     6001 ttttagctgc tcctagttga tgtgctttag ttattaattc attcaactgt tcatagacga
     6061 tcgaaaaagg ttcctgtgtc atgattccgc ctataggaag agaaacacaa gctatttcct
     6121 gctgattgtt gacaacggtc atcccgccac ctttttgaat caaaagatta gcagcataca
     6181 gcatatcctc gtcgtttgtc ccaacgacaa ctagattatg cgagtcatgg gcaatcgttg
     6241 tcgctatcgc tccttgtttc agctgaaatc ctttcactat gcctaatcca atgtttcccg
     6301 tttgatggtg acgctcgatc accgcaatct ttaattggtc tttttcgatt gaaggaatga
     6361 atcgttcgcc ttgtcggtcc actttttcta ccaaatcttt agtcactaga ctattcggga
     6421 taatttcgat cacatgggct tgtgaagaag aaagaagcag ctctaacgaa tgtggattta
     6481 atggttgagc gtttagtttt ggcagacaac acgtatatga atttgaatga tcagcaaata
     6541 aatggtcttg gagttgccca gattgaacaa ctaatgttcc cttatagaaa acttcatgaa
     6601 ttggcagctt ggtaagatcg tctgttaaga agaaatcagc ttgataccca ggggcaatcg
     6661 cacctagtgt tttcaaatca aaacattctg ctgcattcaa tgttgccatc tgaatggctt
     6721 gtaatggatc gattccaagt gcaatagctt gacggacgat atgatcgatt gatccttctt
     6781 ttactaaatc attgatcagt ttatcatcgg tgacaaagaa gcatcttctt gaattttcag
     6841 gtgtgatcgc cggtagtagc gatgccagat cttttgcaac ggtcccttct cgaaccatta
     6901 aatacatacc aagatccaaa cgttctttag cttcttttgc agttgtagct tcatgatccg
     6961 tccgaatacc tgctgcagga taaacattta actcttccat gccgatacca gctgcatgcc
     7021 cgtcaatttt tttcttatgc tggtgcatca attggatctt ttctaggatg tctgtttcat
     7081 tatttgctac tgcttgatag ttcatgactt cagctaagcc taaaacttcg ggtttttgta
     7141 aaaaaggggc taattttttt gcatttaaat ttgcaccatt ggtctcaaaa ggggttacag
     7201 gtacacaaga aggtagcata aaaaagatat tcatgagtgt ttgtcttgca tcttctatca
     7261 taaactcaag acctgtactc ccagcaatgt ttcctatttc atggggatct gtcactacag
     7321 tagtaactcc atgaagcaag gatacttttg aaaattcgct tggtgataaa agtgagcttt
     7381 cgatatgcac atggccatcg atgaatcctg gcactagata ttgcccatta gcattatgta
     7441 cgctttttcc ttcgtattca ccgattccta cgatcattcc ttcgcagatt gctacatctt
     7501 tttccatgat ttctttcgta aagacgttga cgatcttagc atgcttgatc actaggtcag
     7561 caggcttttt tttacttgct acttcgatat atttccttaa tgttgtttcc aatggagtgc
     7621 ctcccaagat aaatttttat aagtataaca taaattcggt ttttgccaat atttttttgt
     7681 aaattcattt tatttgaaac aaaaagatca aaaagacgta tgttttttgt ggaggggaga
     7741 ataatggaaa taaaacgatt atcggatcaa atgataagtg aaagttttgc caaacatgcc
     7801 caagacgtat atatattgcc agcttcagaa gtattttaca tctattttcg ctatggaaaa
     7861 aaacgaacat tctggaagaa agtctcttcg gcagatggac agcaactgat ctcaagattt
     7921 aagtatttgg gacagatgga tatcggagaa aaaagaaaag ctcagttagg tgcatttact
     7981 tattctttag aaaaacagga agttcgtctg cggttgtctt ctgtaggaga ttaccttcaa
     8041 cgagagagtt tagtgattcg gctattatac ggtacttttg aaacacccca tcgttgcttt
     8101 agttctcgag attttgcaac tattttacag aaaaccgtca gacgtggatt ataccttttc
     8161 agcggaccag tcggatcagg caagacttct ctaatgtatc ggttggcttt ggaagaaaaa
     8221 ttgcaagtga ttactatcga agatcctgta gagatagaag aaccgttttt tctgcagcta
     8281 cagatcaatc cgaaaatagc acagacttat gatacacttt tgagactcgc attacggcat
     8341 cgtccagact tgctaatcat tggcgagata agagatgaat caactgcaca tgctgcggta
     8401 agagcagcac taacagggca ccgggttttt gcaaccgtac atgcacgcaa tcttgaaggt
     8461 acattggtca gaatccatga actgacaaag aaaaaagaag agctatctga atgtttagct
     8521 ggtgtcattt atcaagaact tttggaagat gccaatcacc atccttctgt tttatggagt
     8581 tatcaattta tggaggaaga atcagtaaaa aaatcatggg aggaaagtta tgaagaagct
     8641 caggaaaata tttggcagtc agaactattc taaaggatta acaagaagcc agcaaaacca
     8701 gtttgcacaa accatgggag aactgctttt atctggtttt agtctgcaag aatcccttta
     8761 tttattactc acgatccgtt cgtttccacc tgttattttg gcaaatatcc aacatcagct
     8821 aaaggaaggg caagcgttta gtgaggtact ttcgcaaata ggatatggag cagagcaact
     8881 ggtacagatc gagttagctg aacgtcatgg gaatctgccg acaactttgt tggaaattgc
     8941 caaacagttt cggcttgttg aaggatttcg caatgatttg aaaaagctgc ttgcatatcc
     9001 gatgctattg ctcacctttt tgctaggtat cttgtttagt atgcggttgt tcgttttgcc
     9061 tcagcttatt ggttcaggaa tggtcatgca agagcattgg ggtattcagc tgatacaagt
     9121 ctttcattgg tatgttctac tattcatcct agcggttact ctattggctt tactagtaag
     9181 gtatcgtctg atgaagttgt ctcctgttga tcagagtgaa tggataagca gacaattatt
     9241 gattggaccg atatattcct tgtatcaatc ggcttatttg tcactagaga ttggaaagct
     9301 ttttcatgaa ggtttcgaat taaggcaaat catagtctgc ttaaaggaaa cgaaacaaga
     9361 aagtctgatt cagcatcttg cctctaagtt gattcaagga ttggaggcag gtatgtcatt
     9421 ggctgagcag ttccagaatt atcgttttct atcagaagaa ttcagtaaaa ttgttttaca
     9481 gggagaggca aagggaagtt taggtaaaga actgattttt tacagcgaat ggacaagaaa
     9541 acagcttttc aaacgtcttc atcactggat gcattttatt cagccaatca tttttttggg
     9601 cgtagcggct ttgattttat tagtttatgc cgctgtttta ttacctgttt acggaagcat
     9661 agaggaggtt ttgccatgaa aaagagaaaa atatcttatt atagcggttt tacattgatc
     9721 gaaatgctaa tcgtcttact gatcatcagt gtattagtgt tgttgttcgt ccctaatttg
     9781 tcgcgttata ggaatcatgt agaccaagaa agtcgtgaag cgatcatcca gttggtagac
     9841 acacaaaaag agctctatgc gcttcaaaat aatggaagag ttcctactgt ggaggaattg
     9901 ttgaatgaag gctatatcaa acgagaacat gcagaaatct atcagcggcc ttgaaggtaa
     9961 ggttttagca tcaggatata cgttgtttga atcactctta ctgattttat tacttacaac
    10021 gatcctttct tttccagttc tcgcattttc tgcatggaag aatgaattgg cagtctatca
    10081 atttttcggc caatttgaaa aacgaatcta ttctacgcaa aaaatcgcta ttgtgaatca
    10141 gatccaaaca ggcttttatt ggaatagtga agagaatcaa atcatttttc atgttccgaa
    10201 tccagataaa ataaattggg aagtattaga tattcctgat gagattacct tgaaacgcca
    10261 tgcttctatt acttttgctg ctagaaccgg gaatgaaagt tcactaaaag cttatcaatt
    10321 ttattgggaa gaaaaagagc ggacgattac gtatcaattc cagataggga gtggtcgcta
    10381 tacaaaaaga attgaataaa acaggatttg ttttgttgga gtcccttaca gcgcttatga
    10441 tcgcttctgt ctgtgtctac ttagtgagct ttggccagct gtttttgctt caggcagaaa
    10501 agaaaagtgg ggaggaactg ctatttattc gaatgattta tgaagatatc aagacgcagc
    10561 ggatgtatca caaaagacca gagcgaaggg aagaaatgat cccatacgat atccaaattg
    10621 tcgataataa tggagtaatc gaaaaagtgt ttgtcaatat ggagaaagaa gcgatcagta
    10681 ttgaaaaaac aagtaattcc agcatttacg ctgattgaat gtctaattgg actgatgata
    10741 ttgagcagtc tattgctcac attcaatctt ttgattcagc aaaccatcca agtaagtgaa
    10801 tttttgcagc gtaaagatca aaaagagtgg ctaatatttt tagcacaatt agaagaagaa
    10861 ctcaagagca gtcacaatgt ctctgtgaaa aatcagcaac tgcactacac gatcgaggat
    10921 aaacagtata tcatcgaaag atatcaggca atgatacgta aaagaagaac ttccggcgga
    10981 catcaaccta tgttaacaag tatcactgag cttcaattat tggaaaaaga acacacaatt
    11041 tctttttatg tacactttga aaatggagaa gatggatatg ccatatggac aaaaaacgat
    11101 cagtagaaaa tgttataagg gctctatttt attttctgtc ttgcttttat ttcttttatg
    11161 tacgttttta ttgatggcag cattagaaag ctaccgtttg tcaatggatc ttaatatacg
    11221 aatcaaaaat tattatatag caaaaataat gaagcagatg gctttatcgg aacagtctac
    11281 gcttgccgaa agttcggaag gagtttttta ctacacaact ggatctgtaa catatcagtg
    11341 ggtacaacag tccctattct tagaggtgga agtctcaccg tttatctttc ggttcacaga
    11401 agaagtaaaa aatgatacag atactagtac agaatgatgg aatgttcgtg aaaacgccct
    11461 cttttctgtt ataacactgt ttttaataaa aaaatgtttg ccgtttcaca aataaaaaac
    11521 agtgaaaacg attgaaattt tgtcaattgt tcgatagaat taaacaggtg aaaaaattgt
    11581 tataatatat ctagaaatga ggtgcaccat gttaccagaa aaaatggaac aagggtttaa
    11641 tcaaaacctg gaagctattc agcttttaca gaatgcattg ggaacttcct ttttagaagc
    11701 ctatgtcgaa aatgcagaaa atttaataga tgattatcaa gtacgtgtgg tggatggagt
    11761 gccgacaaaa gagacaacgc aacggatcac agctctttat gaagagttga agaaactttc
    11821 tttcgagcca gaagaatggc gcagactgtc tcaattactt ttattgaagg gcagccagac
    11881 agaacatttg caggcgaacc atcaactcac tcctgatagt atcggttttt tatttgtttt
    11941 tcttattgaa caactataca caaataaaaa agcaccggtt aagattctgg atattgcggc
    12001 aggaatgggc aacttgttat taacagttct tttaaaccta tcgaatgcgg ggtaccaaac
    12061 agaaggaatt ggtgtggata ttgatgatac attgttggct gtcgcagctt ctacaagtga
    12121 tttgacacaa gcaaatgttc aatactttca tcaagacggg ttgcaggaat tattaataga
    12181 tcctgtcgat tttgcaatca gcgatttgcc aatcggttat tatccgaatg acgaaaaagc
    12241 aaaagagttt ttaacaagta ccgaagaagg ccatagttat gcacatcatt tattgctaga
    12301 gcaatcaatg aaatatgtga agccggatgg ttttggtttg tttttgatgc cgagtggatt
    12361 tttagaaacg gatcaaagtg aagaaatcaa aaaatggttt aaagaagaag gttatcttca
    12421 aggaatgatt caattgccag atgaattgtt ccgtaataaa caatcgcaaa aaagtatttt
    12481 gatcttacag aaaaaaggac cacaagcaca acaagtaaaa gaagtcttgc ttgtgaaact
    12541 tgcttcatta aaagaaccag agaaggtgac ggaatttttc aacgagttta aaaactggaa
    12601 atcatctgct ctgtaatcat tcaacgagaa ggagagtcat atgtctaaaa caattgcaat
    12661 caatgctggg agttcaagtt tgaaatggca attatatcaa atgccagaag aaaccgtagt
    12721 tgcgaaaggg atcgtcgaac gtatcggttt acaagattct atttttacca tcaagtacgg
    12781 cgataatcaa aaattcgaac aaatcatgga tatcgaaaac cacgaagtag cagtaaaaat
    12841 gctgttagat cagttgattg aactgcaaat tttaagctct tatgatgaaa tcaccggagt
    12901 agggcaccgt gttgttcacg gaggagaaac ttatggggat tcagtagtga tcgatgagaa
    12961 agtaatggac cgcattgctg aattagcaga atttgctcct ttgcataatc ctgcaaactt
    13021 aatggggatc aaagcgttta aaaagatttt acctgacatt ttgagtgtgg ctgtattcga
    13081 tacatctttc cactcaacaa tgccaaagca aaattattta tatagcttac cggtgtctta
    13141 ttatgaagat ttcaaagtaa gaaaatatgg cttccatggc acaagtcatc gttatgtgtc
    13201 ggaacgtgcg gcaaaattat tgaacaaacc gatcgaagaa ctaaaaatca tcacttgcca
    13261 cttaggaaat ggtgtatcga tcacagcagt cgatggaggt aaatccatgg atacttctat
    13321 gggattcaca ccattagctg gggtgacaat gggtacacgt tcaggtgata tcgatccagc
    13381 tgttctccca tatttaatgg aaaaattaga gattgatatt gatgaaatga tcaatgtttt
    13441 gaacaagaaa tctggtctgc taggcctaac aggattgtct agtgatatgc gtgatcttga
    13501 aaagaactat gaaaaagaac atattcgttt agcttatgac attttcgtgg accgcatccg
    13561 aaaatacatc ggcggttatg tgacaacgat gaatggtgtt gatgcaattg tctttactgc
    13621 tggtatcggc gagaatgacg gacatgtacg tagtgaaatc atcaaaggta tgtcttggtt
    13681 tggttgtgaa atcgatccag cgttgaatga attgcgtggt gaagaagtag atatctctac
    13741 aaaagaatca aaagtaagag tattagtgat cccaacagat gaggaactaa tgattgcccg
    13801 tgatgtggag cgtctgcgcg gctaagccaa agcattcgtt aggataaaag atgaaacgca
    13861 tgcaagaggg aattcttgca tgtgtttttt ttgcggagaa gaccgcaaaa tggtaattta
    13921 acttcaattt cccttctttt ttacaggatc ttcttaggga attttgcttg gaaaagtggt
    13981 caaaagggaa aagactggca ttttatttat tgattttcag gaaaatccca atattttatt
    14041 ttcattcatc tttataaggg acattacata tgtaaaaaga aaaaacattt ttagatcagc
    14101 ctcgtgggct gtcgacttta ttcttcgctt tatgtttagc tagaaaaccg atccgaaaca
    14161 ttcattgatc gaataataaa aacttgattt tccacctgta ctttgcctga atcgtgaaag
    14221 cacaggtatg aaaatcaagt tttttgttgc gaaaaaacgt cctagtcaat cagttggccg
    14281 agcggctggc ggctgatctg ctcctgatca ataacgagtg cttcttggaa gtgttgctgc
    14341 aaaaagtagc ggtcatggct gactgctagg agcgttcctt gatagtcttg cagcatttct
    14401 tcaatttctt ctcgagcata gctgtcgagg tgattggttg gttcatccaa aatcaggacg
    14461 ttgatttttt ctaaaaagag tttcagcaac gccaaacgga ttttttcacc accggataaa
    14521 tctttgatcc gtttacccac atcgctttga taaaagccac ttttggctaa ttgccgtcga
    14581 gcgacttctt cgtttccagt caatgtgatg gcgtaagcca gcaaacggct atcgggatcg
    14641 gcaaactgca gctgctgcgg caaataaccg atttgcaagc tactacctaa tttgatggtg
    14701 ccaccgtaag gcttttcctc accgagtaat aatttgagta aggttgattt tcctgcacca
    14761 ttgtcgccga tgatggctaa atgatctcca cgatagactg caaaactgct gtctttgaat
    14821 agtacttgat cagcgtactg ctggtgcaag ttttgaatga tgaaaacttc tttgccagat
    14881 tttccaccat tagacaagct ttgcagccga tttttcgggg gaggtggcgg tttgacgagt
    14941 gtcagtttcg ccaggcggcg ttccagttct ttggcttttt tgaagaagga ctcattgtct
    15001 ccttcatgtg cccactgacg aaaacgacga atcatgactt tcaaccgctg gacttctttt
    15061 tgctgcagtt cgtaattttt tgtcagctca gccaagcgag cttctttcaa gactcgaaaa
    15121 cggctgtagt ttccgggata ctcaatcaat gcaccttctt ccagttccca tgtttttgtg
    15181 gtgacttgat cgagaaattg ccgatcatga gaaacgacca gaaccgattt ttttgtattc
    15241 tgtaaatagt tttccaacca gcgaatactg ttaagatcta aatgattggt cggttcatcg
    15301 agtaaaagta aatcggcatc ggctgctaaa attttggcta aagcgacttt aactcgttcg
    15361 cctccgctaa gttctgttaa ccgtgcttcc tgacggtgtg gaatccctaa gccttgcaag
    15421 atcgctgcaa tccggtcttc taatacgtaa ccgcctagtt cttcaaagcg atgctgcagc
    15481 tcaccgtaac gggccagaca cttctctaag tttgtatctg gttctgtcat caatgcttcg
    15541 tagcgactca tttgctgttt gaggtcctgt atctcagcgg cagaatggag caagtagtgg
    15601 ttgacggtcg cttcggaaac tgtaagttct tgttcgaccg tcccgatctt taagcctttt
    15661 ttgcgactga taacaccgct gtcgacacct tctctgccgg tcaagatttg taaaaaggtt
    15721 gatttgccag ttccgttgat accgactaag acaatttttt caccagcttc aatttgacag
    15781 gataaatcct caaataatgg attcccgccg attgtttttt taatagaaat tgcttgtatg
    15841 atcatgatct ctctccttga gaccactcaa aaaggccatg gttatccatg gcctgtgtta
    15901 tttaaactca gattatcatg agaaaaatac cttgagatgg ccattgattt tacgtgaatt
    15961 caactgtttt ggacaaactt gtcccgtcaa cagcaactgt aaaaccaatt gcatggctaa
    16021 atgcgatatg gtatcgttgg taattttcat tacttgttat tttagtcatg cgcatcatag
    16081 tattttccct ccttcgtgta gttgtctttt ccagtgtacc tgaaagtgtc agaaagttca
    16141 actagcgctg acatttactt aatatttatc gatgtgtaat tctgtcagag agcttttttt
    16201 atctgactga gggtaatgtc tcgtggacca catcattgta ttctgagcta aaccagcgtt
    16261 caggcgaaat catatggtgt ttgataaaaa actttgtcta catgacttcc ttttgccatt
    16321 tgcttaatac cgttgcatta aacagctctt ctctttggaa atctcaccaa catcgcgttc
    16381 aggagtatcg ttgtggttgc aatggcaagc ggtagccttt catcaattct tgaatgattt
    16441 accgaatgta gcacttgcct tctcgcggta cccgaacccg aaataaaatt ttagtttggc
    16501 gataaaatcg gtatccgcca tcgatacttc atattttttc gtatcagatt catcggaaac
    16561 ttccacattg acgaaccaat aagctttggc atgcttaggt cgttttaaac cacaccaggg
    16621 cgactaatcg ccctggtgtt cacttttttt tattttactt gaactcattg actgttttcc
    16681 ctttatttta aacattataa agaccgatga gtgcttgccg cgtcttttgt ttttatcacg
    16741 gttttttgtg caaaaaaacc tgccgatagt tggcaggtcg tctacgtgtt aagctttggt
    16801 gtggtcttta gctggcaatt ggttttctaa atcagttcgg acaacaagca catcgcaagc
    16861 tgcgtttcta ataacatatt cagaaacaga accaataaat aaacgttcta cagcattcaa
    16921 tcctgtggct cctaacataa tcaaatctac ttgattatct tctgggattt ctctggcaat
    16981 aatttgtttt ggtgagccat attctacgac agaggttaca ttattcaagc cagctttctt
    17041 cgcattgctt tcataatctg ccaatgtttg ctttgccatt tcagtagctt gttctgcaag
    17101 cattccatca aaggatgaaa cggtttggaa agaacgtgtg tcaatgacat gtgctaatag
    17161 aagttctccg ttatttcgga tagctacatt gaccgccttt ttaaaagcta attcagcttc
    17221 atcagaacca tctacggcaa ccatgatttt tttgtattgt tgtaacataa gcattcactc
    17281 ctcaagtcaa tcgtattgta ctcttttatt gtagaacact ttagggaaaa agagaattat
    17341 ttactgcttt ggttcagcaa ttttttttgc aaatcggata ctgaaggtgg cagaaacaat
    17401 aattacgatc agtaaaaaga ccaaggcaat aaagcgttga ttgaaaaaag ctgttaaaat
    17461 acccattacg cctaaaacag catatgcagc tccgtaaaat tgtaggaatc cttgattttt
    17521 atcatttttg ttgatcaata caaaaaagat atcctgttta ttaaataaat aatagctaac
    17581 gaataataat actaaagtgg cgataatcat taaaatccta atcatgaaca aatcctccaa
    17641 aataagcaac aaatagaaaa agcaatttgc caagagcaaa ttgctttatt ctttaaaaga
    17701 tggtaatctc cgttaatgcc gttgtttgtc ctgtagtatt gatcccgcga attcaagcag
    17761 gtgggtccca tggtcgtagc ttcgaactac caaatgataa ggcatgttac cagcagtgac
    17821 acaagacggg tcccaagata tcaataaaaa tgttcggtca acacacaaag aggacaattc
    17881 gaacatcaca gatttcccac ctctatagta gcatatgaat ttaaaaagag caatgagaga
    17941 gctcgcagaa acagaaccgt tttcttgtct agttttttgg aggatgcttt ttccactctt
    18001 ttatacggta atactgttga cccagagcct gttcatattt ccctgtatct tttggctgat
    18061 aatactgagc attcttcagt ttatctggta aatattgctg attgacccaa gcctgatcaa
    18121 aatgatgagg atactgataa cccacgccac ggttgagtga tttcgcacct ttgtaatggc
    18181 tatctctcaa atgatcagga acatctccag ctttgccctc tcgaatatca gcaagtgctg
    18241 catctaatgc catgtaggca gaattggatt ttggcgacaa gcaaagatcg acgacgacgt
    18301 ctgctagtgg gatacgtgcc tcaggcaatc ccaatttttc agcagctaat acagcgttca
    18361 ctgtacgagc cgcagcagca ggattaccta aacctatatc ttcataaccg ataaccatca
    18421 gacgccggca aatacttgcc agatcacctg cctcaactaa acgggcgaga taatgaagtg
    18481 cagcatccac atcacttcca cgaatcgatt tttgaaaagc cgaaataacg tcataatgag
    18541 catctccatt tttgtcatgg gtaagagctt ttctttggac acattcttca atgatcgaca
    18601 gtgtaagatg aatccttcct tctttatctg aaggagtcga tagtgtagcc agttccagcc
    18661 cattcaatgc actccgcaaa tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt
    18721 cgtctaactg gattgcttgt tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga
    18781 ctgccaactg gatgtcttgt tctgttaatg gtttgacttc aaagatctgt gtacggctgc
    18841 ggatcgctgg attgatggta atataaggat tctcagtagt cgctccgatc aagatgattc
    18901 ggccactttc taaatgagga agcagaaaat cctgttttgt tttatctaat cggtgtactt
    18961 catccaataa gaggatcact gtaccactca tttttgcttc ttccgctaca acttgtagat
    19021 ccttttttga atctgtagcg gcgtttagca tgcgaaatgc ataattagta gagccggcga
    19081 tcgcacttgc gatactcgtt tttccagtac caggaggtcc atatagaatc atggaagaga
    19141 gcatgcgagc atcgaccatg cggcgtatga tttttccttc cccgactaga tgctgctgac
    19201 caacgacttc atcgatcgtt cgaggtctca tgcgataagc caatggctgt tgcataggat
    19261 tttcctcctt cttgttttta cgtcccttaa ttatatcata tttagcaaga tgcctagaaa
    19321 gaacagcctt ctgttgtttt gttttcatag cttatccaac taatattgtg tatgataaag
    19381 taggtgagaa aaatgacgga taaacaaaag atagatgaat ttttttggaa taaaacgacg
    19441 ccagaagtgg cacgggattt attggggatg tatttggagc atgacacacc agaaggaaga
    19501 ttagctggat atattgtaga cgcggaagca tacctaggtc cagaagacga ggccgcccat
    19561 agctatggtt taagaagaac accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc
    19621 tacctttaca cgatgcatac tcatcggatt ttgaacatca ttactcagcc agaagggatt
    19681 cctcaaggag taatgatacg tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa
    19741 aatcgtggcg gaaaaactgg tccagatatc agcaacggtc cgggaaaatt agtggaagca
    19801 ttagcaattc ctcaagaact ttatggtcag tctatagcag atagttcgtt acgcttagtt
    19861 tttgagaaaa aaaagacacc aaaaaagatc aatgctttgc ctagaatcgg tatcccaaac
    19921 aaaggtgtat ggacagaaaa gccgttgcgt tttgttgtgt cgggaaatcc ttatatctct
    19981 ttacaaagaa aaaaccaaat cgagaaagat tggggttgga ggaaagaaaa tgaaaaagaa
    20041 ggatctatta acatatttag atgaaataat tgaaaaacaa gtgacagact atgatgtagc
    20101 tttagattgg gatacaaaaa atcatacgat cgagatcgtt gttcgcttgt ttgcagaaaa
    20161 taaagcacaa tttgagattg atgatgcaga agggatcgtt tcgcaagaac caattatcga
    20221 atttgaagac ggcgtacttc tttttaatcc tcagaaatct gtttttgatg aacgcaatta
    20281 tctagcagta atcccttatg aaggaaaaaa agggttggca aaatcagtag ctgattcttt
    20341 agtggaatat ctaaatgaag tattagctca agggcaaagc gatttgcttg attttttgga
    20401 tgaagacgat gacgaagctg tattcgagct tcactgggat aatcagcaac tagcagaatt
    20461 agtagaagta aaacagcaaa atgaagcaga tacttattta ccgtacccaa gttattaagg
    20521 agtgacagat atgaaatgga atgaagtaaa agtagaaact gaaagtgaag cagtcgaagc
    20581 agttgctaat attttgatgg aggcaggggc gagtggtgtc gcgattgaag attctttaga
    20641 tgttgaacat tttcagtcgg atcaatatgg tgagatttta gataaaaatg attttacatc
    20701 gctgaaagaa ggcgcactag tcatggctta ttttccggaa acgactttct taccggaaat
    20761 cttgcctttt atcaaagatc gtatcacaca attacctgag tttggtctag ctattggaaa
    20821 aaataaagta acagttagtg aagtagagga aagcaactgg gcaacagcct ggaagaaata
    20881 ctatcatccg gtgcgtatta cgcgaatgct gacggttgcg ccaagctggg aaaactacca
    20941 gacagaagat cctctagaaa aaattatcac actagatcct ggtatggcat ttggcacagg
    21001 cactcatccc acgacttcct tgacattgac agcactagaa acagtcttgc gtggtggtga
    21061 aacattgcta gatgtgggaa ctgggtcggg cgtgctaagt attgcagcaa aatatcttgg
    21121 tgcaaaagat gtctatgcct ttgatttaga cgaagtagcc gttcgttcgg cgaaagaaaa
    21181 tatggatatg aatgaaatag ccaaagatgt ccatgtatca gctaacgatc ttttgacagg
    21241 aatcgagatt gaaagtgatg taatcgttgc gaacatttta gcagatatca tcttattgat
    21301 gatcccagat gcttggcgtt tactgaagca aacaggcacg ctgatcgttt caggaatcat
    21361 tgaagagaag aagcaaatgg ttctggatgc catgttagaa caagggttta tcgtggatca
    21421 aattttccag caaaaagact ggtatgcaat tattttgaaa aaaccagagg tggaatagat
    21481 gcagcggtat tttttaaaag aatcatatga atcaaaagac tattttgttt tagaagggga
    21541 aaactatcac cacattgtac gggttatgag gatggaaccc gaggatgaag tttttttggc
    21601 attcaatgat caaatagcca ttgttgcaga aatcacggag attactgaag aggctgttta
    21661 tctgaaagaa attaaaaaag aagaaaatca aaaggaactg ccaagttcga ttaccatagc
    21721 gagtggctat cctaaagggg aaaagctgga ttggatcgtt cagaaatcca ctgaattagg
    21781 agcgcaagct tttcttggtt tcccggcatc agcctctgtc gttaaatggg atgcaaaaaa
    21841 acgtatgaaa cgacaacaac ggcttgaaaa gattgctcaa gaggcagcgg aacaatccca
    21901 tcggcagatc gtgccatcag tagagttatt ggaaacgaaa caagatttat ttgagcggct
    21961 agatgattat gacgtggtct tggttgctta tgaggaatca gcaaagcaag gcgaaaaatc
    22021 aaatgcagcc aaaattctta gtgaaacaaa accagatagt cgaatcttag ctgtctttgg
    22081 accagaaggt ggattatccc gcgatgaagt cgattatttt gtagaacaca aagggatact
    22141 ttgtggatta ggaccaagaa tccttcgtac agaaacagca cctctttatt tattaagtgc
    22201 tgctagctat caatgggaat taatgaatcc ttctttcagc taacgttctt cattaataaa
    22261 agagtatcga cagaaagaat tgaaaaaata gtcaacaatt tgtataatag aaacaattat
    22321 ttatacaatg gatacgaaaa atcgtttgaa aaagctttta aaaagcgaac atttccgtaa
    22381 aaaaacgtat aactgcacgg attttgggac aattatcaaa cagatatttg tcccgattcc
    22441 cgttttaccg aaaaaagaaa gggggatcga ttcctgtgac aaaagaagaa attttaacgg
    22501 gacccggtgt cataaagctc gttagtcaat acatgggtcc cgaacatgta gcatttgttg
    22561 agaaagcatg cgaatacgcg actgctgcac atgacggaca gtttagaaaa tcaggcgaac
    22621 cctacattat tcatcctatc caagtcgcag gtatattagc agatttaaaa atggatcccc
    22681 atacagtggc tacaggcttc ttacatgatg ttgttgaaga tacagaaatc actttagaag
    22741 atctgagaga agaatttggc gatgacgttg ctatgttagt ggacggcgta accaaattag
    22801 ggaaaataaa atataaatcc cacgaagagc agctagcaga aaaccaccga aagatgctgc
    22861 tagcaatggc tcaagattta cgagttatca tggtcaaatt agctgataga ctacataaca
    22921 tgcgtacgtt gaagcacctg cgagaagata agcaaagaag gatcgctcag gaaactttag
    22981 aaatctatgc gcctcttgca catcgtctag ggatcagccg gatcaaatgg gaattagaag
    23041 atacagcact tcgttatcta aatccaaaac agtattaccg tatcgtccac ttgatgcaga
    23101 cgaagagaga agaacgtgaa aaatacgtaa gtggtactgt tgaagatatt cgaatagcga
    23161 cagaagagct ggggattttt gcagaaatct atggacggcc aaaacacatt tattcaatct
    23221 atcgtaaaat gaaagatcag aaaaagcagt tcaacgaaat ttatgacttg ctagctattc
    23281 gagtgatcgt agattcaatc aaggattgtt atgctgtatt aggagcaatc cacacaaaat
    23341 ggaaaccaat gcccggcaga tttaaggatt atattgccat gccaaaagct aatatgtacc
    23401 aatctttgca tactaccgtg atcggtccgg caggaaatcc ggtagaaatt caaatccgaa
    23461 cacaggaaat gcatgaaatc gctgaattcg gggttgctgc acactgggcc tataaagaag
    23521 gaaaaaatga aaaagtagaa ccagatggta tgacgaaaca attaagctgg ttccatgaga
    23581 tactcgaact tcaagacgaa agctatgatg cttctgaatt tatggaaggc gtaaaaggag
    23641 atatctttag tgataaagtc tacgtcttca caccaaaagg agacgttact gagttaccaa
    23701 aaggatccgg accattggac tttgcataca gtatccatac agatatcggt aacaaaacca
    23761 ctggtgcaaa agtaaatggc aaaatggtgc agcttgatta caaattgaaa aacggagata
    23821 tcattgagat catgacttct ccaaattcat ttggcccaag tcgcgactgg ttgaaattag
    23881 ttgctactag caaagcaaga aataagatca aacgtttctt caaagcccaa gatcgagaag
    23941 aaaatgtgat caaaggccac gaatccgtgg tcaaatgtat tacagatcta ggatttacgc
    24001 ctaaagatat tttgacgaag aacaaactgc aagaagcact cgatcgtttt aattatcaaa
    24061 cagaagatga tctctatgca gctgtagggt atggagaagt tagccccttg acgatggcca
    24121 atcgtctgac tgaaaaagaa cgtaaagaac aaaaaatcga gcagcaaaag caagaagcag
    24181 aagaaatcat gaatcagccg aaaaaagaac ctgacaaaat gaaagtacgt catgaaggtg
    24241 gcgttgtcat tcaaggggta gaaaacttac taattcgtat cagtcgctgc tgtaatccga
    24301 ttcctggtga tgatatcgtt ggttatatca ctaaaggcag agggatatcc attcatcgtc
    24361 gagattgtcc gaatgttcag cctgacaaac caaatgtagc agaacgtttg attgaagtcg
    24421 aatgggaaga tacatcgaat acacgaaaag agtatgatgc agatttggaa atttacggct
    24481 ataatcgttc aggcttattg aatgatgtac ttcaaacagt caatgcgcta acgaaaaatc
    24541 tcaacagcgt tgaagcacgg acgaataaag ataaaatggc gacgatccat ttgacggttg
    24601 gtatccagaa tttatcccac ctaaagagta tcgtggataa aatcaaagca gtacctgatg
    24661 tctacagtgt acgccggacg aatggatagg aggacagata tgagagcagt tgttcaacgg
    24721 gtaagtaaag ctgctgtgtc agtcgatcag caagaaattg gaaaaattgg ctacggccta
    24781 cttgttttat taggcattca tgaaaccgat acgcaaaaag atgtggacta tctggtaaaa
    24841 aaaattgcac aaatgcgtat ttttgaagat gaacaaggaa aaatgaatat tagtgtagaa
    24901 gatgtgggag gacaaatttt aagtatctct caattcacac tgtttgcgga tacgaaaaaa
    24961 ggaaatcgac caagctttgt ggaagccgca cgaccagaaa cagctattcc tttatacgaa
    25021 tcattcaatg aaggaatcag aagtcgcggc atcacagtcg ctaccggaca attcggagca
    25081 gatatgtcgg tttctctcgt caatgatgga cctgtaacga tcattattga ttctaaaaac
    25141 aaataaagta atctggtttt cactagtaaa aactatgaaa cacgtcgctt aagttagact
    25201 gataaactct aacttaagtg gcttttttgt ctgcaaaaag cgcttctttt gtcagcagtc
    25261 ggaaaaaaag ttaagcttaa attatacaag ttatttttta taggaggttg ctgaagatga
    25321 aagaagtacc agtagtagat atcaaaatca cagatttaaa aaaattgtat caaaaaggat
    25381 acaacatgtt ggaggagtta cgccatgaag cggacgctcc cgtcgtcaaa gcaaagattt
    25441 tcaataaaga agctatcacg atctacggat catcagcagc aaaagtattt tatgatccaa
    25501 ggaatttcaa acgaaaaggt gctatgccaa aattagtatt gaagacactt tttggtcaag
    25561 gtggagtaca gactttggat ggagctgctc atcaccatcg gaaaaatatt tttatggatc
    25621 taatgacacc ggaaagaatg gaagattatc atcgtatctt agataagaat ctgacacaag
    25681 cacttgaagc acagcatggt caatttgaac tatttgattt aagtaagatg gttttcttta
    25741 catctatttg cgagtgggca ggtatcaatt tatcagcgat ttctaaagat gaggtcgaaa
    25801 aattagcaga atatcaaatc tctatgatca gcggaacgtt tacttctccg attgatcaca
    25861 taaaaggtgt cgaaaatcgc aaaaaatcag aaaaatgggc gcaaggcttg attgaagaag
    25921 ctcgacaaaa tcctgtggct ggaaaagaaa atgtggcatt atatgctttt gccaatgcca
    25981 cagatttaga tggtcagctt ttacctctgg aagtggcagc tgttgaatta ttgaacatca
    26041 tccgtccaac ggttgctcta actgtgtggg cggcattgat gggacatgca cttttcagta
    26101 gaccagattt gtatcagcaa ttaaaaaatg acttttcgac gttacaagat ccgtttattc
    26161 aagaaatgag acgctattat cctttcttcc caatgctacc tgcgatttcg ctgaaagaag
    26221 tagaagtaga tggttatcgt ataccagaag gcagttgggt tattttagat ttatatggaa
    26281 cagaccatga tgaacgaaca gttgaagcgc cagattcctt tatgatcaaa cggtatgtag
    26341 ggaaagcaaa agatatttcc tataaagaag aatatgagat gattgcacaa ggcgggggaa
    26401 attttcgaca aatgcatcgc tgtgcaggag aatggatcac tcttcatagt ctacgtgtgt
    26461 tttccgatca gctagtaaat aagtttgagt tctctgtacc tgaacaagat tggacgattc
    26521 cattcaatca gtttccgact tatccgaata gccgagcgct actttacaag aattagatta
    26581 gcataaactt atcgaaaaaa aggaagatca aacagtgctg atcttccttt tttcatatta
    26641 ctataaattg ataaggtcaa ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata
    26701 atcttcattg gataaatgca aatctaaagc ttggacattg tcagatactt gctctggacg
    26761 cttagctcca ggtataacta cagacactcg tggattcttc atgtaccaag ctaaaacaag
    26821 ttgcgcagga gtggcttggt atcttttagc aatggggcga agtacatcga cagcggttaa
    26881 aatttctctg aaacgttctc cttggaaatc tggatttctt tttcgaggat ctccttcacc
    26941 aaattgtttt tcctcaccaa gttcatattt tcctgtcagc aaaccggaag caagaggaaa
    27001 atagggaaca aaactgattt tgtttttttc aagataaggg aataattctt tttcagcctg
    27061 ccgatggatc aaactgtatt tatcttctac tacatcgaca tatccatctg cattcgcttc
    27121 tttcaattgt tctaacgtga agtttgacac accgactgct cggatttttc ctgcttcttt
    27181 taattcgtgc aatgtagcaa ctgattcatt ttttggtgtg ctttcatccg gaaagtggat
    27241 ataaaaaatg tcgatgtaat cagtttgcag acgttttagt gcatcttcca ccgattgttt
    27301 gagaaactca ggagaattat caaatgttcg ccctttattt ggtacatgag cagcttttgt
    27361 agcgatgacg acacgtgagc gatcatattc tttttccttc agtacctcgc caatcagttc
    27421 ttcggaacgg ccgtttccgt aagcataagc ggtatcgatc aaattgatcc cgctattcaa
    27481 agctgtacga actacttgtt ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc
    27541 tccaacggca tttgctccta atccaagctg ttctgcgtag acttgtgaat gtccgattct
    27601 tatttctgcc atagctgatt cctccttttt gtctagtcta aaacaaaata ccaaaaagct
    27661 gaactaaaac gtttttgtga ggggaaatag tcagatatct cttgactttg agactggttt
    27721 ttagtaggtt ataagtaata tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt
    27781 acaagagagg atcatcgagg ctgaaaatga tctaaagttt cattagccta aagacactct
    27841 ggagatggac gagtgaaaag ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa
    27901 atctttcaaa attaggtggt accgtgtatg aatttcattc acccttgttc atgatgaaca
    27961 agggtgattt ttttatttac atagctaaga aagggagaga aaactatgag tttccaaaga
    28021 ccaaaaggaa caaatgatat tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa
    28081 acagcacgtt tattattcag agattatcag tataatgaaa tgcgtacacc gatttttgaa
    28141 cactatgaag tgatatcccg aagtgtaggg gatacaacgg atatcgtttc aaaagagatg
    28201 tatgattttt atgacaaagg ggaacgccat gtgacgcttc gaccagaagg tacagcacca
    28261 atcgttcgtt cttttgttga gcataaattg ttcggcccag agtatgcgaa accgttcaaa
    28321 actttttata tgggaccaat gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa
    28381 ttccatcaaa tcggagtaga agcatttgga tcagaaaacc ctgcaactga tgtggagagc
    28441 atggtgatgg cacttgattt cttcaaacag ttggggatca cgcaaatccg gctagtcatc
    28501 aattcattag gtgataaaga aacgcgtcaa gcctatcgtc aagcattgat cgactatctt
    28561 ttaccttttg aatcagagct gagtgaagac tctaaacgcc gccttcatga aaatccatta
    28621 cgcgtattgg acagtaaaga caaacgagat caaaaatttg tggcagaagc tccatctatc
    28681 ttagattatc taagcgagcc tgctcaaaaa cattttgata ccgttgtttc catgctggat
    28741 gcattggctg ttccttatga aatcgacagt aatatggttc gcggactaga ttattacaca
    28801 catacgattt ttgaaatcat gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc
    28861 gcaggcggac gttatgacaa tctagtggaa gaactaggtg gtccttcaac acctggcttc
    28921 ggatttgcaa tgggtatcga gcgattactc ttgactatgg aggcagagga agtagttatc
    28981 cctgcattta atgagttgga tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa
    29041 gcattgaaag tggtccaagc gattcgaaac tttggttttt cagctgaccg cgacttcatg
    29101 aaccgtaaag caaaagcgca atttaagaca gcagataaag cgaatgccaa attagtattg
    29161 gtgatcggtg aagatgagct tgctagtcaa acgattaatg tcaaagcaat ggcaagccga
    29221 aaagaaaaaa gttttccatt agaagagata tatgaacatt ttgatgaagt ctatgatgaa
    29281 atgactttat tcgagtagga ggaaaaaaat aaatggcaca aagaacgatc tattgcggat
    29341 tagtttccga acaacaatta gaacaaacag ttacattaaa aggatgggta caaaaaagac
    29401 gagaccttgg gggagtaatc tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg
    29461 tctttaatcc tgaaatcaac aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat
    29521 acgtgatcga agtgactggg gaagttagaa gacgcggaga aaatgcagta aatcctaaaa
    29581 tggccactgg tgaattcgaa gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa
    29641 ccccgccatt tcaaattgaa gatgatcaag caatcagtga cgaaatacgt ttgaaatatc
    29701 gttatttgga tctgcgtcgt ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa
    29761 caaaagcgat tcgtcattat ttagatgata atgactttct cgatatcgaa acaccttacc
    29821 tagggaaatc tacaccagaa ggcgcaagag actacctagt tccttcacgt gtccatgctg
    29881 gacatttcta tgcgttgccg caatctcctc aattgtttaa acaattgctg atgaatgctg
    29941 gattcgaccg ttattatcaa atcgttcgtt gtttccgcga tgaagattta cgtggagacc
    30001 gtcagcctga atttacgcaa gtagatattg aaacaacgtt cttaacagca gaagaaatcc
    30061 aaacttacac agagggattg attgcgaagg taatgaaaga agttcgcggt atcgaagtca
    30121 cattgccatt tcctcgtatg acctatgatg aagcaatggc tcgctacggc agcgacaaac
    30181 cggatacacg ttttgatatg gaattgatcg atctaagcga tacagtcaaa gaagtagaat
    30241 tcaaagtctt ccaaatggct cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag
    30301 gcgctgcaga tcgctactca cgaaaagaca tggatcagtt aggtcaatat gtaggacaat
    30361 tcggagctaa ggggcttgca tggctgaaag tagaagaaga cggattgaaa ggtccaattg
    30421 ctaaattcat gggagaagca acagaagcaa tcattaaagc aacagatgca aaaccaggtg
    30481 acttgctgat gtttggcgca gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc
    30541 ggacacgttt aggaaaagaa ttaggactaa ttgatgaatc gaaattcaat ttcttatggg
    30601 taactgattg gccacaattt gaattttcag aagaagaagg tcgatatgtc tctgctcacc
    30661 atccattcac gatgccaaaa gaagaagata tcccgttatt ggctgaagac cctgcaaaag
    30721 tctatgcaga agcttatgat atcgtcttga acggttatga actaggcgga ggttctatcc
    30781 gtattcatac tcgtgagctt caagaaaaag tactagaaac tctaggcttt acaaaagaac
    30841 aaatggaaga acaatttggt ttcttgttaa cagctttaga ttacggattc cctccacatg
    30901 gcggtattgc attaggattg gaccgttttg caatgttatt agccggagaa gataatattc
    30961 gtgaagtcat tgcctttcct aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa
    31021 gtatggtttc gcctttacaa ttgttcgaat tgaatattga tgtaacgaat atagaagaat
    31081 agccgataaa aaggtaaaaa ggatttgtcg aatacgcttc ttttgacaac cttgatttaa
    31141 ttgaaaaaca ctttataatg aagccataac atataaagcg gggcttttcc atgaaaaaaa
    31201 cattcgaaaa ttgggcttat catgattaca cgaccaagct ttcggtatct atcgtttatg
    31261 cgttgatcgc atcagtcgca ttgaatttct tttatcagcc aggacatatt tattcaagtg
    31321 gtattactgg tctagctcag attttgacga ctttgtcaaa atcactgata ggattcgaag
    31381 taccggtatc aacaactttg tatttactga atataccttt atttgtattg gcgtggttaa
    31441 aaatcgggaa gaagtttaca attttcactt ttatcacagt cactttgacc tccctttgta
    31501 tccatatttt accggaaaca gtcctatcta ccgatccaat catctgtgcg ttgtttgggg
    31561 gggctgtgat gggactaggt attgggctag cgttgagaaa cggcctatca tcgggcggtc
    31621 tagatatcgt gagtatcacg attcgtaaaa aaacaggaaa gtcgattggt tctatttcga
    31681 tctacttcaa tgcattgatc gtttttgttg ccggaattct atttggctgg caatatatgt
    31741 tttacagtac gttgtctatt ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac
    31801 aaaagaagat gcaggtaatg atcgtaacga aacatccaga aaaagtagta aatgaaatcc
    31861 aaaactgtat gcgtagaggg atcacgatca tcaatgaagc agaaggtgct tataagcatg
    31921 ataagcagac agtattgatg acgatcgtta cacgatacga actgcctcta cttagagaag
    31981 caatgaaagc ttctgatcca caagcttttg taagtattgc agaaaacgtc cagatacttg
    32041 gtcgtttcta tgaggatggc atttagatcg agtttgacaa aaattcatga aatactgctg
    32101 tagacagacg ttcgtttgtc tgcagttttt ttctaccata gtagaatcag caaagaaaaa
    32161 ctgctttttt atttgaaatt ttcttaaagt tgaaaaaaag catgtttatt tttttaaatt
    32221 tatgggatga tagaaaaaaa tagtagagaa aggacacgat tctacttgct attttcactt
    32281 aatacccgta tattacgaat gaaaaaagtt tatgaaaagt tggaatattg aatggaaaaa
    32341 caaaaaaggt taaaaaacag ccgctacatc actgggtttg atgggctccg ttcattagct
    32401 gtggtgggtg ttatcttcta tcaccttctg ccaactagta tgaaaggcgg ctatttgggc
    32461 gttccgattt ttttcgtcgt atctggttat ttgatcacgg atctgctgcg gcaggaatgg
    32521 gaacaaaatg gaaaaatcaa tatttggcaa ttttatgtca gaagaatgaa gagactctat
    32581 ccggcacttg tttttatgct tgtgacagct tcggcttata tcactttgtt tcagcgaggg
    32641 ctgttaaaca atcttcgagg agttgtggtc agcagcttac tgtatgtcaa caactggtgg
    32701 caaatcaata acggtttatc ttatttcgat cgatttgcta atgagtcacc gtttacgcat
    32761 atctggtctt tagcagtaga aggtcaaaac tatttggttt ggccgatctt gtttgtattg
    32821 ttgatggtat ttgttcgtaa aagaaaatgg atcttttata ctgtgttagg tacatccttc
    32881 ctctccgcca tcttgatgat gatcttgtat acaccaggag gcgatccaac ccgtgtttat
    32941 tacggtaccg acactcgtct gttttctata tggttgggaa gtgcgcttgc gtttgtttgg
    33001 ccaagtacgc gtttaaagaa aaacatcccg aaacaagcga aaaatattct gaatatcgct
    33061 ggaggaatct cgcttttggc actgattctt tgcttcttct ttttagatga tcattacaat
    33121 ttcatctatt acggaggaat gctcctagtg agtctgttat gcgtagtgct agttgcagtg
    33181 acagcacacc ccggagcaag tttgaatcga tggctgacaa atccagtatt tacttggatt
    33241 ggcaaacgaa gctatggcat ttatctgtat cagtttccag ttatgatttt ttatgaagca
    33301 aaggtgactg atattgccga tcatgttttg ttgcatactt tagtggaaat cacattgatt
    33361 ctaatcatca gtgaactttc ttatcgatat gtggagcgtc cgctagcacg ttttgattat
    33421 ggtcgtacat tccaagtgat gaaaggatgg ttcagcaaac cgatcatcag taagagcaaa
    33481 ccatgggttg ttccaggcac attacttgta gcaattgcac tcgttggttt cgttacggca
    33541 ccgaaaaacg ctgtaacagc cgatcaaaaa caactgcagg caaaaatcgc tgaaagtaaa
    33601 aaattagcag aagaaaccag aaacaattca gaaaacacca ctagttcggt tgaccaagcg
    33661 atccttgata agtacgagtt gtccaaggca caagggaata aggcacaaaa attagaacta
    33721 acggcatttg gtgattcagt tatgttggat gctgcaacgg atctacaaga actttatcca
    33781 aaagtagtgg tagatggaga tgtcggacga caattatatg ccagtgtacc gtacattgaa
    33841 aaattaaagg aacaagattt gttgaaagac actgttctga tcggactagg aacgaatggc
    33901 tcattctcgg aagctcagtt tgatagtgtg atggatgcaa tcggtgatcg aaaagtatac
    33961 tgggtcaatg tccgagtacc gacgaagcgc tggcaaaatg acgtgaatgc gatgcttgaa
    34021 cagatgacga aaaaatacga caatatgacg ctgattgatt ggtacagcta cagtaatgac
    34081 catagtgact ggttttatga tgatcaagtc catccaaatc ctgaagggat gacacattac
    34141 attcacttag tcagcgaagc cttgctagga ggaagttcaa gtacgaacag cagtgagaca
    34201 aacgtgacag aatgatagct acaaaaaact tcaggcaaaa acatttgttt ttgcctgaag
    34261 tttttttata tttaccgtgt ttttaacatt catatagatt caggaagcaa aacggttgag
    34321 tgtgtctgta aataacgttc tttttgtttt cgggtgagtt ttttgaatgc agcttctgct
    34381 tttgttgctt cgcttcttga agaaaatact tctgcatgga tcatcttaac aggtcttctt
    34441 ttttgaagac gagtatattt agcaccagta ccattgttat gttcctctaa tcgtctagaa
    34501 agatcggttg tatatccacc ataaaatgtc tgatctttac agaataatac gtaaaaataa
    34561 tgttcatttg ccatagagca tctcacgaat ctctgcagtg tattctccat ccatattgta
    34621 agtgatcaat ggtggagcga ctttgaatcc gtctaatttg ccttctttga ttccttcaac
    34681 taacaaaata tttgcctctt ttccaggttt tgggtaaaca aattggatcc ttttaggtgc
    34741 aattcgatag tcttccatga cctgaatgat ttctaagaac cgatcaggac gatggaccat
    34801 agtaaagcgt ccatttgttt ttagtagctt acttgaaata tcgatcactt cttctaacgt
    34861 cgtatgaatc tcatgacggg cgattgccag gtgtttatta gggttcgttt tatttgttgg
    34921 cagccctttg aaataaggtg gattacagac gactaagtca caagaattat gtcgaacgac
    34981 agataagctg tctttcagat cgatggtgtg gacagtcatt tgttcattta aatggttaag
    35041 cgcgatacta cgtcttgcca tgtctgctag acgaggttga agttcgatag cgtcgatagc
    35101 cgcctgggtt cgtttactta aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac
    35161 aatcttccct tttttaggta cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa
    35221 agaaaacact ttggaacttt ggataatctt gatatcttct gcgtataatt ggtcgattcg
    35281 ttcgtcttcg tgaagcatgg attccctcca aaatgaaaaa aatagtcgtc tgtattatac
    35341 tatttttatt aaaaagaacc aaggtggctt gtcaaacctc tattttttat gcatactatg
    35401 accataagaa gggaaaggaa gagactatgt tttatcgatt tatgcgcggg gtagttcgag
    35461 tactattagc cttagttaat ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag
    35521 atgagaatta catactagtt gctccccatc gtacttggtg ggacccatta tatctggcgg
    35581 tagctgcagc acccaaggaa ttcagcttta tggcaaaaga agaactgttc aaaaatccag
    35641 ttctgcgatt tatattaact cgatccaatg cttttccagt taaaagagaa aatccaggac
    35701 caagcgtcat taaaacacct gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt
    35761 ttcccagtgg gacaagacat gctacagaac ttaaaggcgg aatggcattg atttccagaa
    35821 tgtctaaagc gaaaatcgtg ccagctgtat atcaaggtcc gttgacactt ggggatctgt
    35881 tcaagcgtca gagagtgaca gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga
    35941 aaacggataa agaaggtatg gcggagatcg aacgacgaac acaagcagct tttgacaaat
    36001 tggacaaaga agtgaaccct gatttcaaat atgaagtcaa aaaaaaataa aatgataagt
    36061 ttatgcgtgg gcggtctttt gaatctactg tcctgttcag ttcagacttt ttcaatgaaa
    36121 acgagggaga gacgtcaagt ctgccctctt tttgcgttcc aatatgtaaa atagaagaca
    36181 ggtgtcatgt aatggatcaa agtgttataa taattagaag gacatttttt gaggtgattg
    36241 aatgcgtttt ttacatacag ccgattggca tatcggaaaa aaactacaag gttatgactt
    36301 gattgaagac cagcgtcatg tactatcttc tatactagat atcgcgaaag aagaaaaagt
    36361 agaggcaatc gtgattgccg gagacttata tgataggagt gtcccagctg ttgatgccgt
    36421 agaattatac aatgaattga tggtcgattg gaatttaaag gaaaagatcc cgatttttgc
    36481 tatatcagga aatcacgata gcagtacgag actttcagcg ggtacgccat ggttcagtta
    36541 ttcgaactat tttttgcgaa cacagctagc agaagcattt gaaccagtcg agtttaaaaa
    36601 cgtacaattt ttctttcttc cttactttga accaattgca gcgagattat attttgaaga
    36661 tgacaatatc cgaacgatca agcaagcggt agccaaggtg atagataaaa tgcaggagct
    36721 atttgattca gaaaaaaaac aagtattgat tagccatttt tttgtaatgg gaagtgtaaa
    36781 atctgattcg gaaacgaaaa tcgaagtagg cgggttggat tcgatccctg gtgaactact
    36841 tacaccgttt gattatgtag cactaggaca tttgcataat caagctgctt tgagacagga
    36901 taatgctcgc tatagcggtt ccccattgaa attctcttta tctgagatca accaaaaaaa
    36961 aggcgtatgg atcgttgacc tgacaacagg cctggagttg aattttaggg agatcgaacc
    37021 cctgcatgat attgaagaaa taacagcgag tttcaaagaa ttgctggatc ctgcctttta
    37081 tcagtccgtc aatcgggaga attacatcta tatctatctc actgatagaa cgatcatccc
    37141 gaatatgatg aaccagcttc ggcaaatata tcctcgtatt ttggggattg aacgggcaaa
    37201 cggtcgagca gaacaaacaa agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa
    37261 attagcagaa gcatttttta gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa
    37321 atggctcgga gaaacgttgc aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa
    37381 aaaattacaa cttaaaaatt ttggtccttt tatcaatgag accgtagatt tttcacgttt
    37441 gacagaagca ccgctttttt tgatcagcgg gaagacagga gctggcaaaa cgacgatttt
    37501 tgatgggatc accttcgcac tttttggtga aacttccgga agacttcgct ctggaaaaga
    37561 gatgcgttct ttgtttgcta caccagacga agaaacaagc gtgacttttt cttttgaaca
    37621 tcagcagatg agctatgaaa tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg
    37681 aaacggaaca aaaaaacagg cagccaaagt aacgttgacc atttttgatg ggaaaggaaa
    37741 agaaataaaa caaatcacaa aacgtacaga agcagatcag ttgatcagag aactgatgaa
    37801 tctcgatgcg aaacagtttt ctcaaatcgt tttactaccc caaggagaat tccgtaattt
    37861 tttggtttca tcaagcagtg ataaagagac tgttttacgt catttattcg gtactcaatt
    37921 tttccaacag ttcaatgagc gattaaaaga aaaagcaaaa caacagcaac aaaaattgga
    37981 tcatttagag caagagctgc atcttctgca aaaaaggttt gttttagtca aagaagagga
    38041 accagcagca gggtttgaag ccgttttagc ccaatggaca aaccatcagg caatactcga
    38101 aaaacaaatc acggaagaaa aagaagctct aacggaactt caggaaaaac aaaagcaact
    38161 agaaaattcc tactatcgtt ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt
    38221 aataaacaag caaaaggaat tagctgctca aaaagacgaa atagaggaaa agaaacgatg
    38281 gattgggtat tatgaattta cgcaacgatt agtcgaacca atccagcgcc tacaagaaat
    38341 aaaagttgaa aaaatagaac tgtcacaaaa aagtagtgag caaacaaaga aactagcgat
    38401 gactacgcag aattatgaag agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac
    38461 aaaaatcaaa gaagctgctc aaaaactgca aaacaaacag atgatggtgc caattgtcga
    38521 atccttacgt agtaaaaaac aagacgcaga acaattaaca atttcattgg ataaagaaga
    38581 attgaatgcc gagaagctgt atcaaaatct cgctatctat caggaaagaa ctcgaattct
    38641 tcaagaaaat ttactggatc aggaaaaact gcaagaagat cgtcttgctt ttggacagtt
    38701 aaaatatcta gatgaaaagc agcaagaaat agaaaaacaa aaagcaaaag acactgggtc
    38761 gctcgcacag ataagagaag aactcaaaaa agcaactgaa gaaataacaa aaaatagtca
    38821 gctattgaaa gaacagacag aacaagcaga acagctgaaa agccaatggg caaagcagca
    38881 aattgctcgt cttcagcttt ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac
    38941 ggaacacccc atacaacaat taaaacacga acagccaaat aaggaagata tcttaaaagt
    39001 agaacaacaa ctggcagaag ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa
    39061 aagtgaagca catgaaaata atcttcaaca acaattatca gatttagaac aaaaaatcat
    39121 ggcaacgaat atacaattgg ctgaacaaga agaacacttg caacaggctt tgaaaaaggc
    39181 atttcctgag ataacagaac aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt
    39241 agatagacga aaaagcaaga tggccaatgc aagaatagag ctggaagaaa taagacaaca
    39301 agcagaaaag ctcgaacaac aattagctgt ctacaaacag cagcagacag aaaaaaataa
    39361 tcagctccag caactgcaag gagaaatcaa aagtttagaa caacaattgc aagatacgga
    39421 tagtaactgt ttagaagagg agatacgacg acttgaaaaa cagttagaag aagactcgaa
    39481 tcaactagct gttgagcaga aagcaggagt gcaattgttg caagatcttc atacgcttcg
    39541 gacacagctt gatcttcaac atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga
    39601 acaagaaaga atagctaaaa agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat
    39661 ctgtgcattt gcacaacagc aagcacaata taaagaaaac caacgtacag ttacttctta
    39721 tcatgaagag caattagtga cagctgaccg gctgcgtcaa ttagaaaata tccagctaac
    39781 tgagacattc ccagatacag aagagatcag ccaacagtta gaagaaataa gacgtatgat
    39841 cgaaataaag caagaaaaga tatacggttt acaagaacaa aaaattagta atcaaaaaat
    39901 ctacgatgaa tgtctttcga tttatcacac aagccagacg aaattagatg agcttacgca
    39961 attacagcag ctttcgcaaa cattcaacgg agaaaatcct aagaagacta gtttggaacg
    40021 gtatgtgctt caggtttatc ttcaagaagt gttacaagta gcaaatgatc atctccaacg
    40081 tttaacgaaa aatcgttatc aatttgagct agcggatacc attggcagtt atcgagggaa
    40141 aaccggatta gaaatcaata tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac
    40201 cctgtctggc ggtgagagtt ttattgcagc actttcttta gcattagctc ttgccgatgt
    40261 gatccagaca caagctggtg gtgtagcaat cgatgcattg tttattgatg aaggatttgg
    40321 gtcattagat gaagaagctt tggaaatggc aatggaagct ttagaaacga tcgaaagtga
    40381 aggaagaatg atcggcatca ttagtcatgt aagagaattg aaagaacgag tattacaaca
    40441 aataagaatt gatacagaag gtagcggaca aagcaaaata cgctatcaac ttggataaaa
    40501 agggagattg gtaactatga aatggagtat tccagaaaaa gtcattgcac gtggacgtca
    40561 atacttgcaa gaaggccgag tgttatcggt tgtccctgac caagaagaaa acgtgtggca
    40621 tgcagaagtc ttgggcagtg aattgtatat ggttgatttg gatggtacag caaaagagat
    40681 agattactgc caatgtccct attgggagga acataaatat tgtaagcata cagttgctgt
    40741 ggaactttat ttacggtcga aaaatctatc tcgtatcatg aaaaaagacc agccaatgct
    40801 agaaccggta aaagcaacaa gtgagggaga aatcattgca aaaggttttt ctcgtctaaa
    40861 aacacaaaac aatcctcaag cggtgcaacc tttgattatt gaataccaag tagaaacgat
    40921 tgagactaat cagtatcacc cagagctttc tattttggca ctttacttgc gtatcggttc
    40981 attggcatca ccgaaaaaaa catatatcgt aaaaaacatc tatgattttt tacaagctta
    41041 cacagaaaaa gaaacttaca ctgttaataa acagtaccag tttcgtctag ataaacgggc
    41101 gtttcaaaaa gaagatcaaa aaatactcac gcatcttgca ggaagtgctc agacgcatca
    41161 attattaggt acgaacggct tacaagtcaa aggaaaacta gataaaagat atctgcttct
    41221 cccagtagaa caagcccgct tcctgttaga gcagatgaat cagactacac gtttgcatct
    41281 gcagataaat gaacaaaacg atcaaggaat acaattttcc gatcaaaaca aacctttatc
    41341 attcaaagtg gaaaaatttg gagaagatta tttgctgcaa gcattgaatg attttgattt
    41401 tttctttatg cattatcagt ggggagttat ccaagggaca ttttattctc tgacgaaata
    41461 tcaacaaacg gtttatcaaa cttggaaaca attgattcgc cgattggaga aaccagaagt
    41521 agtgttcaag aaaaaagaat taccgtcttt gtttaaagag attatccctc tgctgtcaga
    41581 gatcggcgac gtagctattg cccctgcagt agaagaggaa gtagcagata tccccgtcga
    41641 atttattttc tttttccgaa aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta
    41701 tggcgaagtc atctattcga ctgacccgaa gcatgaggtc tcgacagaac aggcttttca
    41761 ggtgttacgt gacacagtag aagagaaacg agtacttgat ctattcagaa tgtatcgcta
    41821 ccagaaaaat gaaactggtt atgaacgtcc attgccaagc ggggaagaac tatatgcctt
    41881 tttccgcaca gaactatctg tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt
    41941 gcgagagttg tatttagatg cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg
    42001 ttcatggctc gatattcgat tcgatgtgac agggatagag gagcaagaaa tcgatcatgt
    42061 cctgcaaagc ttgctgagaa atgatgcttt ttatacatta gaaaatggtc aggttctttc
    42121 ttttgattca gaagaatttc aacaaacaag ccaagtgttg caacaattac gggaatcgat
    42181 acgtacagaa gaaggaacga tccatgtacc taaaaatcaa ggattgatca ttcaaaatca
    42241 attggaaaaa agcaatgcta cttttagtga atcgttccaa acgatggtac aagatctcat
    42301 tcatccagaa agataccagg cacagcttcc caaaggattg aatgcaacga tgagagatta
    42361 tcaaaagcaa ggttttcgat ggttgaagat gttaggtcat tatcagtttg gcggtatttt
    42421 agccgatgaa atgggacttg ggaaaacact acagacaatt gcctttttac tttcggaaaa
    42481 agaagaacgg aaaagcttct ctgctttgat cgtagcgcct gcgagcttaa tttacaactg
    42541 gcaggcagag gtgagaaaat tcgccccttc tttaagtatc caagtaatca atgggaataa
    42601 aaaagaacgt gaagaattac tggctaaaga cacagatatc cgagtgactt cttatgccag
    42661 cttaagacag gatctagcta attatcagtc gcaaaaaatt gattatttga ttcttgatga
    42721 ggcacaaatg gtcaaaaata gcagtacgaa aactgcacaa gcattgagag aattagctgt
    42781 acctcagcgg tttgcactga gtgggacacc aatcgagaat aatttagaag aattgtggtc
    42841 attgtttgca acgattatgc caggattttt cccgactaaa acaaaattca gggaacttgc
    42901 cgcagaagaa attgcgcaaa tgattcgacc gtttatttta cggcgagata aacaaactgt
    42961 gttgaaagac ttgccggaaa aaacagagat gaatctatat tctgcactta cagaagaaca
    43021 aaaaactgtt tatttagctt acttgcgtca aatgcgtgaa gaaatcacat caatggattc
    43081 tgaagcattc aagaaaaacc ggatcgggat acttgctggt ctgacaagac tgcgacaaat
    43141 ttgctgcgat cctcgtctgt ttattgaaga ttaccaaggc ggttccggca agctcgaaca
    43201 agtaaaagat ctactggtag ctgccaaaga gaacaaacga agagttttgc tgttttctca
    43261 atttacaggg atgctgacta tcttgcaaga agagttagcc gagctaggga tctcgacatt
    43321 ttatttaaga ggcagtacga aaccacaaga tcggttaagt atggtagacg catttaatgc
    43381 tggcgaaaaa gatgtttttc tgatttcctt aaaggctggg ggaactggat tgaatctcac
    43441 gggagcagat acggtcatct tgtatgacct ttggtggaat cctgccattg aagaacaagc
    43501 tgctggacgt gcacatcgga tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc
    43561 tgaaggaacg attgaggaac gcatggattc actccagcaa gaaaaacgcg aactgttcca
    43621 aaaagtgatc caaggtaatg aagaacagtt gacgaaactg actgaagaag acatccgcat
    43681 gattttaagt gtaggagaaa tcgaggagta agagactcga aaaagcgttc agtaatgggt
    43741 aagaaagcct ttctaaagaa gcgattgttt atttgattta aaaaatagca aggaggctgg
    43801 gattccattt tatcccagct tccttgctat cattttttta tttctatttt taaaggttat
    43861 acaaagattg tcttcattgt ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa
    43921 attggagtct gttcaatttt gcgttctcga tggttgaacc aaagcgcttg ccagttagca
    43981 ctttttgcac ctagaacatc attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga
    44041 agcatatgaa attctttctc agctagctga aaaatctctt tttctggttt ttggaaacct
    44101 gttgcttgag aaatgagcat attgcgggaa ggaatccaat tgttcaattg aagttgcttc
    44161 agtttttttg tctgatggtc agtcggccca tttgtgataa ttcctaatgg gacattcttt
    44221 tccttcaaga aatcgagggt ttttttgacc tcctcatgca agcagatatt atcaagttct
    44281 tcctcgtaaa ttttttgaaa taagaggcca tcttcttccg taatatgagg gtagtctaaa
    44341 tcttttagtg attggctgat ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc
    44401 attacttttg ggaagttttc atcactatga tggcggaacc ggatatatag tggatgcata
    44461 tctgcatctg aaacaagcgg aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga
    44521 tcatacatcg tatcatcaac atcaaacaca actgatttaa tcaaaatttt acacgccttt
    44581 cggttttcat tgattttttc acaaatcctt gactattgta acagaaaaaa ttgtctaaaa
    44641 aagctgataa ataaaggttt ttcttactat atttctattt ccttgacaga ataaggtttt
    44701 tatatttcag tttttttcac taaaaatcgt gaaacacgtc aataagtttg atgaacaaat
    44761 taatcattat ggtagaagtc gattgattta ttttaatgca atctctgtgg aattttcgaa
    44821 agatttat
//
LOCUS       DAZAID010000013        41707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000013 DAZAID010000000
VERSION     DAZAID010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.013"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACG9BE_000654"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACG9BE_000654"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFN3031432.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACG9BE_000655"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACG9BE_000655"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFN3031433.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACG9BE_000656"
     CDS             complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACG9BE_000656"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFN3031434.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="ACG9BE_000657"
     CDS             2179..2499
                     /locus_tag="ACG9BE_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFN3031435.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3468)
                     /locus_tag="ACG9BE_000658"
     CDS             complement(2611..3468)
                     /locus_tag="ACG9BE_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN3031436.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="ACG9BE_000659"
     CDS             complement(3576..4880)
                     /locus_tag="ACG9BE_000659"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN3031437.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="ACG9BE_000660"
     CDS             complement(4877..5386)
                     /locus_tag="ACG9BE_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031438.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="ACG9BE_000661"
     CDS             complement(5561..7696)
                     /locus_tag="ACG9BE_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFN3031439.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="ACG9BE_000662"
     CDS             complement(7779..9434)
                     /locus_tag="ACG9BE_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN3031440.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="ACG9BE_000663"
     CDS             complement(9436..9750)
                     /locus_tag="ACG9BE_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN3031441.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="ACG9BE_000664"
     CDS             9920..10180
                     /locus_tag="ACG9BE_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFN3031442.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="ACG9BE_000665"
     CDS             10194..10508
                     /locus_tag="ACG9BE_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFN3031443.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="ACG9BE_000666"
     CDS             complement(11238..11792)
                     /locus_tag="ACG9BE_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFN3031444.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="ACG9BE_000667"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="ACG9BE_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="ACG9BE_000668"
     CDS             complement(14153..14869)
                     /locus_tag="ACG9BE_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFN3031445.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACG9BE_000669"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACG9BE_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFN3031446.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACG9BE_000670"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACG9BE_000670"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFN3031447.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="ACG9BE_000671"
     CDS             complement(18339..19799)
                     /locus_tag="ACG9BE_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3031448.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="ACG9BE_000672"
     CDS             complement(19789..21084)
                     /locus_tag="ACG9BE_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN3031449.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="ACG9BE_000673"
     CDS             complement(21416..22093)
                     /locus_tag="ACG9BE_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFN3031450.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="ACG9BE_000674"
     CDS             complement(22123..23214)
                     /locus_tag="ACG9BE_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFN3031451.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="ACG9BE_000675"
     CDS             complement(23315..23749)
                     /locus_tag="ACG9BE_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN3031452.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /locus_tag="ACG9BE_000676"
     CDS             complement(24333..25790)
                     /locus_tag="ACG9BE_000676"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3031453.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="ACG9BE_000677"
     CDS             complement(25787..27682)
                     /locus_tag="ACG9BE_000677"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN3031454.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="ACG9BE_000678"
     CDS             complement(27740..29515)
                     /locus_tag="ACG9BE_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFN3031455.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="ACG9BE_000679"
     CDS             complement(29565..31061)
                     /locus_tag="ACG9BE_000679"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3031456.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="ACG9BE_000680"
     CDS             complement(31255..32877)
                     /locus_tag="ACG9BE_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN3031457.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="ACG9BE_000681"
     CDS             complement(33205..34518)
                     /locus_tag="ACG9BE_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFN3031458.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34763..34987)
                     /locus_tag="ACG9BE_000682"
     CDS             complement(34763..34987)
                     /locus_tag="ACG9BE_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031459.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35007..35429)
                     /locus_tag="ACG9BE_000683"
     CDS             complement(35007..35429)
                     /locus_tag="ACG9BE_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3031460.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35571..36281)
                     /locus_tag="ACG9BE_000684"
     CDS             complement(35571..36281)
                     /locus_tag="ACG9BE_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFN3031461.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36299..36796)
                     /locus_tag="ACG9BE_000685"
     CDS             complement(36299..36796)
                     /locus_tag="ACG9BE_000685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3031462.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36833..37285)
                     /locus_tag="ACG9BE_000686"
     CDS             complement(36833..37285)
                     /locus_tag="ACG9BE_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN3031463.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37420..38025)
                     /locus_tag="ACG9BE_000687"
     CDS             complement(37420..38025)
                     /locus_tag="ACG9BE_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFN3031464.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38475..39095)
                     /locus_tag="ACG9BE_000688"
     CDS             complement(38475..39095)
                     /locus_tag="ACG9BE_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3031465.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39097..39966)
                     /locus_tag="ACG9BE_000689"
     CDS             complement(39097..39966)
                     /locus_tag="ACG9BE_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFN3031466.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(39989..40396)
                     /locus_tag="ACG9BE_000690"
     CDS             complement(39989..40396)
                     /locus_tag="ACG9BE_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN3031467.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40415..41704)
                     /locus_tag="ACG9BE_000691"
     CDS             complement(40415..41704)
                     /locus_tag="ACG9BE_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN3031468.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        11759 a         8983 c         7022 g        13943 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    34621 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
    34681 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
    34741 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
    34801 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
    34861 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
    34921 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
    34981 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
    35041 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
    35101 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
    35161 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
    35221 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
    35281 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
    35341 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
    35401 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
    35461 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
    35521 tttacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt ttataattct
    35581 gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg
    35641 attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc agcattgatg
    35701 gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca
    35761 cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat ttcttccgga
    35821 agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc ttgggctttt
    35881 cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg tacgccaaaa
    35941 gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa ttcccgttct
    36001 tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt agtcttgaat
    36061 gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta
    36121 aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt ttcttcactt
    36181 tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt tgcttcagga
    36241 aatacttccc agaaattggt atccactatt acttttttca tctgttcaca cctcttgatt
    36301 agaataattg cttgatgaat agatagtctg tttggggatc atctcccata taaaaaacat
    36361 gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt
    36421 cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca atagctgtat
    36481 ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag attcgctcga
    36541 tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt agtttcaggt
    36601 aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta tcttttagct
    36661 cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa tgctccggtg
    36721 aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta
    36781 catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga
    36841 tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat
    36901 ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat
    36961 tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat
    37021 agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt
    37081 ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt
    37141 tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg
    37201 ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg gcaatcattc
    37261 cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt
    37321 cacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc ttttttaata
    37381 actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt
    37441 tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa
    37501 ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc
    37561 ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc
    37621 tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt
    37681 ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc
    37741 accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt
    37801 tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag
    37861 cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca atgaaatcgt
    37921 ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg
    37981 agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt
    38041 tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa
    38101 atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc
    38161 gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc
    38221 caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg
    38281 acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag
    38341 tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg tgactcggag
    38401 ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt tttcgatatt
    38461 aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga
    38521 gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat
    38581 ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc
    38641 cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa
    38701 tgacgatctg attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct
    38761 gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct
    38821 ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac
    38881 aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct
    38941 tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg
    39001 gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca
    39061 ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg
    39121 actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta ctgcttcatt
    39181 tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt
    39241 agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt
    39301 attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg
    39361 gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt
    39421 gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac
    39481 aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc
    39541 ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc
    39601 atattctact actgcttttg tcttcaggat attttctttg aaaggcagat gggacccatc
    39661 atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa agtctcgggc
    39721 gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt
    39781 gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgatcgg
    39841 cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt tatgcgtatt
    39901 aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt
    39961 atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg
    40021 caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag
    40081 cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc
    40141 cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga tcgttcacat
    40201 acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta tgcttatctt
    40261 gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt
    40321 ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct
    40381 taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa actgggctta
    40441 agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattgt
    40501 tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac
    40561 catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc
    40621 ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga
    40681 acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa
    40741 tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag
    40801 gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta
    40861 caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca
    40921 tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag
    40981 tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt
    41041 cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa
    41101 aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa
    41161 tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg
    41221 cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc agaccggcaa
    41281 aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa
    41341 tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg
    41401 tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa
    41461 tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta
    41521 aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta
    41581 acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa
    41641 tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc
    41701 ccatgtt
//
LOCUS       DAZAID010000014        41467 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000014 DAZAID010000000
VERSION     DAZAID010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.014"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..329
                     /locus_tag="ACG9BE_000692"
     CDS             30..329
                     /locus_tag="ACG9BE_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3031469.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            594..1469
                     /locus_tag="ACG9BE_000693"
     CDS             594..1469
                     /locus_tag="ACG9BE_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031470.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1466..1684
                     /locus_tag="ACG9BE_000694"
     CDS             1466..1684
                     /locus_tag="ACG9BE_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031471.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1671..4298
                     /locus_tag="ACG9BE_000695"
     CDS             1671..4298
                     /locus_tag="ACG9BE_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFN3031472.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4313..4531
                     /locus_tag="ACG9BE_000696"
     CDS             4313..4531
                     /locus_tag="ACG9BE_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031473.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            6050..6364
                     /locus_tag="ACG9BE_000697"
     CDS             6050..6364
                     /locus_tag="ACG9BE_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031474.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(6417..7190)
                     /locus_tag="ACG9BE_000698"
     CDS             complement(6417..7190)
                     /locus_tag="ACG9BE_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031475.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            7295..7540
                     /locus_tag="ACG9BE_000699"
     CDS             7295..7540
                     /locus_tag="ACG9BE_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3031476.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            7696..8547
                     /locus_tag="ACG9BE_000700"
     CDS             7696..8547
                     /locus_tag="ACG9BE_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFN3031477.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            8580..9257
                     /locus_tag="ACG9BE_000701"
     CDS             8580..9257
                     /locus_tag="ACG9BE_000701"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFN3031478.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            9745..10710
                     /locus_tag="ACG9BE_000702"
     CDS             9745..10710
                     /locus_tag="ACG9BE_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFN3031479.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            10732..10944
                     /locus_tag="ACG9BE_000703"
     CDS             10732..10944
                     /locus_tag="ACG9BE_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFN3031480.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            11019..11495
                     /locus_tag="ACG9BE_000704"
     CDS             11019..11495
                     /locus_tag="ACG9BE_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFN3031481.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            11492..13300
                     /locus_tag="ACG9BE_000705"
     CDS             11492..13300
                     /locus_tag="ACG9BE_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFN3031482.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            13377..13592
                     /locus_tag="ACG9BE_000706"
     CDS             13377..13592
                     /locus_tag="ACG9BE_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFN3031483.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            13684..14547
                     /locus_tag="ACG9BE_000707"
     CDS             13684..14547
                     /locus_tag="ACG9BE_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFN3031484.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            14584..15120
                     /locus_tag="ACG9BE_000708"
     CDS             14584..15120
                     /locus_tag="ACG9BE_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFN3031485.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            15134..15556
                     /locus_tag="ACG9BE_000709"
     CDS             15134..15556
                     /locus_tag="ACG9BE_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFN3031486.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            15486..17885
                     /locus_tag="ACG9BE_000710"
     CDS             15486..17885
                     /locus_tag="ACG9BE_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFN3031487.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            17917..19908
                     /locus_tag="ACG9BE_000711"
     CDS             17917..19908
                     /locus_tag="ACG9BE_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFN3031488.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            19931..20182
                     /locus_tag="ACG9BE_000712"
     CDS             19931..20182
                     /locus_tag="ACG9BE_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFN3031489.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            20172..21401
                     /locus_tag="ACG9BE_000713"
     CDS             20172..21401
                     /locus_tag="ACG9BE_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFN3031490.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            21398..23479
                     /locus_tag="ACG9BE_000714"
     CDS             21398..23479
                     /locus_tag="ACG9BE_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFN3031491.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            23628..27548
                     /locus_tag="ACG9BE_000715"
     CDS             23628..27548
                     /locus_tag="ACG9BE_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFN3031492.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            27549..28493
                     /locus_tag="ACG9BE_000716"
     CDS             27549..28493
                     /locus_tag="ACG9BE_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031493.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            28497..28700
                     /locus_tag="ACG9BE_000717"
     CDS             28497..28700
                     /locus_tag="ACG9BE_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFN3031494.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(29000..29236)
                     /locus_tag="ACG9BE_000718"
     CDS             complement(29000..29236)
                     /locus_tag="ACG9BE_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3031495.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(29419..29790)
                     /locus_tag="ACG9BE_000719"
     CDS             complement(29419..29790)
                     /locus_tag="ACG9BE_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFN3031496.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(29787..31115)
                     /locus_tag="ACG9BE_000720"
     CDS             complement(29787..31115)
                     /locus_tag="ACG9BE_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN3031497.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(31076..31405)
                     /gene="mobC"
                     /locus_tag="ACG9BE_000721"
     CDS             complement(31076..31405)
                     /gene="mobC"
                     /locus_tag="ACG9BE_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFN3031498.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(31663..32033)
                     /locus_tag="ACG9BE_000722"
                     /pseudo
     CDS             complement(31663..32033)
                     /locus_tag="ACG9BE_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            32178..32363
                     /locus_tag="ACG9BE_000723"
     CDS             32178..32363
                     /locus_tag="ACG9BE_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFN3031499.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            32521..32688
                     /locus_tag="ACG9BE_000724"
     CDS             32521..32688
                     /locus_tag="ACG9BE_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031500.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            33306..33965
                     /gene="vanR-B"
                     /locus_tag="ACG9BE_000725"
     CDS             33306..33965
                     /gene="vanR-B"
                     /locus_tag="ACG9BE_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFN3031501.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            33968..35329
                     /gene="vanS-B"
                     /locus_tag="ACG9BE_000726"
     CDS             33968..35329
                     /gene="vanS-B"
                     /locus_tag="ACG9BE_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFN3031502.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(35420..36721)
                     /locus_tag="ACG9BE_000727"
     CDS             complement(35420..36721)
                     /locus_tag="ACG9BE_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN3031503.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            37000..37806
                     /locus_tag="ACG9BE_000728"
     CDS             37000..37806
                     /locus_tag="ACG9BE_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFN3031504.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            37824..38531
                     /locus_tag="ACG9BE_000729"
     CDS             37824..38531
                     /locus_tag="ACG9BE_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFN3031505.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            38528..39499
                     /locus_tag="ACG9BE_000730"
     CDS             38528..39499
                     /locus_tag="ACG9BE_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFN3031506.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            39492..40520
                     /gene="vanB"
                     /locus_tag="ACG9BE_000731"
     CDS             39492..40520
                     /gene="vanB"
                     /locus_tag="ACG9BE_000731"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFN3031507.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            40526..41134
                     /locus_tag="ACG9BE_000732"
     CDS             40526..41134
                     /locus_tag="ACG9BE_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFN3031508.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(41200..41373)
                     /locus_tag="ACG9BE_000733"
     CDS             complement(41200..41373)
                     /locus_tag="ACG9BE_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031509.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT        11746 a         9384 c        10403 g         9934 t
ORIGIN      
        1 gaataattaa ttaaaaagga gaggtttttg tggaagcaat aaaattaatg atttcagaag
       61 aacaggagga aatgtttcta aagcagtttt atcaaattgc tacaaaagct attgaaaagg
      121 ctcaagaaaa tgcggggatg acaagggaat atctcaatca aaaagaaatg gcggaatact
      181 taaatgtgag tgaaaacttt ttaaaaaatg ctgtgagaaa tgagggattg cccgtagttg
      241 aattaggggc aagaaaattt tattcgaaaa aggcagttaa tgagtttatg tttgctcgaa
      301 tgaaaagagg ggaaaatatt ggaagatgat gaagtagaaa ttataaaaaa atgctttaaa
      361 aaaagaaaaa aagtgttccc aaaagtttgc agactggggg aacactcaat aataaagtgc
      421 tgagaaaact ttatttatag ctattatatc aaaaaagaag gtgaaaaggt gttttcttct
      481 ggaaatgttg tagttgacaa acttagagaa ataaattttg acggaaatgt aatccacaca
      541 catggtacga tcaattaaga aaaaaaacga aaaaaggtgt tgagaagccc tatttgaatg
      601 caatagttat cttagcagaa attacctatt ggtatagacc taaaaaaatt tttaacgatg
      661 atggtcagct acttggttat caaaaaaagt ttcttgaaga tattttgcaa aaatcatata
      721 agcaattaag caaaaaaaca ggattatcag aacgagctat aaaagatgca attgtgtact
      781 tagagaaaaa ggggattatc aaaagggttt ttagaaatat ttattatgag aaaagagcag
      841 ttacattatc aaatgttatg tacattgttt taaatgtaca taaattgata gaaattactt
      901 ttcctacttt ggattgcgaa aaaaaggagt atatgccgaa aattcgtcag aggggtatcg
      961 aaaaaaagag atatgtctct ttaaaaaaag gtagaggtat gtacaaattt gtatatacgt
     1021 atacaaagac tacttcacag aacatatcaa agactactct cgttatggaa gaagaggata
     1081 ttaatataaa gaggatttac agtaagatta gtgttcttta tgacgcaaat aacgaagatg
     1141 gtcgaaaatt atttcaagtg tatcactatt tgagaaacgc taatgtagat acagaagata
     1201 tcagaaaaat cattgaatat ttatctgaaa atactcagta tcttattgct gagtgtatta
     1261 taaaacaaca tgaagcatgc ttttttcaaa gaaaaactga agaagggtta tacgattatg
     1321 taacatattt tttgaatgga cttcagaaaa gatataaaag tcaattttta gaaaatgtag
     1381 agcctataga tcagttcaaa aaagctttcg aaatcaaaga gttacctgaa attactatgc
     1441 ataattggtt gaaaggagaa gaatcatgat taaaaatcgc ttaatagatc aaatgtggtg
     1501 tcttgatcaa aatacagata ctctcgaatt aatcgagagt atctgcttca acacagtagt
     1561 tctcgattta agagctgaaa atgacaattg tgtaactcat atcataatta atcaaaaaat
     1621 ggctcagcag ctctctgaga gcctaagaaa atgggctgaa ggagaaaact atgaaagcaa
     1681 ttaaagaaaa aatcgttagt agaaaaataa actggttagc actcagtgta cttgttttaa
     1741 tgattagcgt tgtttttaca agtaatattg ttgaagctag cattttcaat tggtttgatg
     1801 acacaaaaag tcaaacagag tttttgaata attctcttta tagaccttac ctaaaaaaat
     1861 acaatggaga tttccttagt cagttcggga tttggcttgg atgggctgtg gtaaaaggca
     1921 tgtttactgt gactgattcg attcaaaata tgattccaga tgtgttggat ttgttcaatt
     1981 tcattgaaag tacagggcta aataatgttt atcaatcagt cataaataca attgtcgttg
     2041 gattaatgat cctttcattg atgtttgttg gctacaagat gattactgga aaaggaacaa
     2101 tagatttaag atctgttgga atgaatatag tgatgtctgt agcgttaatt cttttaatgc
     2161 caacaatgat ttctagcggt attcaatttg ccaaaatatt ttacaatgat gctactacga
     2221 ttacaaattc agatgacgga gtcgcttggt cattgataaa acaaggagta acagatctgg
     2281 catatattaa taaaacagat caatactcaa gcattgataa aacggaggat cgaaataagt
     2341 taacacgaaa aaattttcaa cagacagatt taacacaagt gttgacggat aaagtgattg
     2401 ataaattgga aaaagaaaat ccagctgcgg ataatcttcg ctatgaatta gttgaaaatt
     2461 ctaataatga atttgttgcc acaaagtttt ctgataactt cttatcaaca ttttctgata
     2521 gcttgaaatc aggttattat cgatatcaag caaatctttg ggggatttcg attgggttga
     2581 cagcgttggc aatcgcctat gtgttcagcg cgtttgtgat cattacagca atattagaac
     2641 ttgcatttaa acgtgtgtta ggtgttcttg tttttgcgac agatattgaa acaggacaac
     2701 gttcaaaagt tgttttatcc gatattttac aatgttactt aacagttgga tttcaagggt
     2761 ttggactatc tatgtttgct atgtttatca attttttaaa tgctggacaa ggtatctcca
     2821 caaatatttt tataaaaaca attgcttata tctgtgctgt gtttgtgctg ataaaaggca
     2881 gtggaacagt catgagatac tttggtgttg atattggact caaagaaggc tatgggcaac
     2941 ttgcttctgc ttttggtatg ggcgcaatgc tgtttagaaa aggatcaact ggatttaatc
     3001 gtgctaaagg aagcggaaat ggaaatggat cagacagtgg agaaggagaa gatcgaaaac
     3061 cagaaaagaa tttcggcgaa actctatcaa aaaaagctgg caaaactgga agagctttgg
     3121 gatatgccca tgaacgtgga ttatcaggtt tagcttcaga tggagctaca atggctagtg
     3181 aaagagcaat aaaaccattt aagagtatgc gtgatatggc taatgatacc aagaataagt
     3241 tcaaagaagg tttagatgat ggtacagtgt ccgctattaa taaaaatagc aaacctatgt
     3301 tagctaaaaa taaagaagat gaaactggaa aatatgctga ttccatgcca acacgtcttt
     3361 cggatagaaa agatggtgct aaggttgaaa atgccgatag aattatgtct agttctgaac
     3421 gtatgcgtga agcaatgaaa aataatgctg aatcgaataa taatcctgta agtgcaattc
     3481 agcaaaaagt tcaacaagat attgaagaac gtaaaaatgc tatgcacggt caagctaagt
     3541 ctgctgaaga attaattaat cagaagcgac aagaagctaa atacacgcct gaagcaatga
     3601 atcgtgagga aatgttaagg aaacgtgtag aagggcgtac aggagctaat acggacgaaa
     3661 aagaagcgtt gaccaaagaa agaatccaag aagctaaaaa ctcaaatacc ggattagaaa
     3721 aattggtcaa agaaaatcta caaaattcta caagtagtca aggtggccga tcagtagatg
     3781 tccgagaaaa tattcaaggt tcattgaatg gtcatgtcgg acggaatgtt gatgtaagag
     3841 agaatttaca aagctcttca aatggtcaag gtactaagac aattgatgtt cgagaaaatg
     3901 tacaaaattc tacaagtagt caagggttta aatcaatgga tgtaagagag aatgtacaaa
     3961 attctacaag tagtcaaggg tttaaatcaa tggatgtaag agagaatgta caaagttcta
     4021 caagtagtca aggattcaaa acagtagatg ttcgggagaa cgttcaaaaa gatagtggag
     4081 aaatgaaaga aaaaacacaa aaaatcaata tcgttgaaga cagaaagtca gcgaaaaaat
     4141 ttgattcaaa tcatgaaaca gtgattattg atagtgaaat ccgtgaaaat gataagggat
     4201 caaaaccaag acgaagattt acctatgaag acaacgagtt attcagagat acacttaatg
     4261 atcctaatcc gcttttcgat aatctattaa aaaaataaaa gggggaattg tcatgaaatt
     4321 aattctaaag attagtacgc atgatttctt agaacttgac gaattatgtg aatcagtaaa
     4381 gaacgcaaca aatgaaaaaa tcacggtcac taattatatt gaactgatga ttcaagaaga
     4441 attaaagatg aaacatgaga taaaaaacaa cttgaaatat tgttatctag aaaaaagtga
     4501 agagataatc catgaattaa atggctttta aaaattgtat ttgacatgaa tttacgacaa
     4561 ggaattagtg gggattaagc gattattctc ttccacttta gagaaataat gggttaaaaa
     4621 gccagaatac gtagagcaat attgacaacc tataaaggag ctattgttga aaattgaagt
     4681 aacaaaaaga taaagggagg tttacatcta tcagattaaa agtgatagta cacccatgaa
     4741 acctgcaaaa gagttgaacc taatgtatat cggtttaact ctttttattt gcaaattaaa
     4801 aaaatatcac cgaagtgacg aatgaaacta tgaccaaatt aagcaagaaa atatatcttg
     4861 caaataaagt agtataaaaa gatattgata aagccaatca aaactttgaa tttcaaacgt
     4921 tagataatca aacagataca aaaaatgaaa tcagttctac gcgtacgatt attaaaaata
     4981 ataaattttc actagaagaa aatggaaata aaatcaaata attaggaaga aaaaacaaag
     5041 caattagaaa agcctgcaag caaatttgag taatcgagat ccaatttttt taaggaagtt
     5101 taacaaaaat atgtaattct attatcacta ttaaagagaa agttggagcg gaatataaag
     5161 aaaagagaaa agtaattaaa cgcaaaaaaa tgtagaactt aaaaaatata aagaggaaca
     5221 accgttaaaa ttaggaatga tgttgtgtaa acattataat tgctatgata taaatgaagt
     5281 gttaaagaaa gcaaatttaa accaattttg tgaacagaaa agccttagtc actatgacca
     5341 aaactacgga cgaaactatg accaaaagta ggtactaaat tatgaccgaa aataggtcca
     5401 aaatggattt ttttatgata tcaaaaatta tgaaatgatt ttgaaagaaa accagttggc
     5461 acagccaact tagccacctt cctgtaggaa ggtgctatca gttttccctt atgctctttt
     5521 ctttcttttt tgtttcatat gtcaaatgcc attttgaaat gtaccatctt aagaaattta
     5581 tccttaaaaa caattgcgtt attggataaa aagatgctac attatcttaa aaaaggaatc
     5641 aaatatgatg aatttagtgg ctagcatatc gtgtgattaa gatactttat agtgtaagtt
     5701 gaaggagaaa aaatatttaa aatatacttc ccatttcagt gggaagtaaa attaatcttg
     5761 ttgtttctac tagtaatgta tctttttgga aactatgtaa cgatgtaaac aagatttttt
     5821 tcagaagaaa aaacgataaa agttgtgata aacgatttaa aaaataggag aaaatatatc
     5881 aagcgcatgg tatgaaacgc acatttaaaa agattcgact aactaaaaaa ataagtttac
     5941 aaagcattgt tgtgtattac aagtgatcta tacactgaaa aatgatagag aaaatctgat
     6001 acgtccgcaa caatggacaa ttgaaaatga aagtgaggaa attggaagaa tggctgatga
     6061 aaataaagtt gttccgcttg aaacactagt gataaataag gaagagtttg agcaactttt
     6121 tcttgatttt tttgttcaat atgacaggat ttatgatttt agttcgtcac tagatttatt
     6181 tgaattagag gcagaaataa tagaaaacag tcgagaggag aatggtaaaa tcatcatttc
     6241 agtagaaaaa ttcaatgaat tattctcaga aacatttgaa atagatacag attattatca
     6301 accacaagaa gttttccaat catttagaaa tcagattatt cgctcaggta agaccgtaaa
     6361 ctgaaaaaca ataaaagaaa cgttgaaaga ataattattc cttcaacgtt tcttttttag
     6421 aatgttttaa tgaccaaagc agtttcattg tcaggcttag gtaattcatg aaatcccgta
     6481 tttatagtgc tcaattgatc aactaaccat gttttacttt ttgaattgta gataaatcta
     6541 aaattaatat cgttattatc gtgttgaagg ggcgtctcca aataatctgc tgtatattca
     6601 tagtcaacgt aacctgctaa tacaaaagag agtatccctt tatttaatgt tagatttaat
     6661 ggataatcat acgactttat ttttttcaac ttcatattgt acatatcttc agccataaaa
     6721 gttgagttcg tgatttcatc ttgggtctca ttaaaaaagt tatccgaata atcgcttatt
     6781 tttgaaaagt ctttattggc accagcctca aaaaattgtt caactttttc agtataaagt
     6841 tgtgctaacg ctcctttttc aatattaact ttaatatttt catgtctatc aatttgtgtg
     6901 ctgttcagta agggaagatc agcttttagt tttatatcgt cttgatttct ttcaatttca
     6961 attggcaaaa tcttttcagt agattcagtt tctgataaag gaacatctag ttttttatca
     7021 tgataataag cttcaaatcc atctggaacc ggaaaaaaat gtattgggta attcttttct
     7081 gaggttgttg tactagtttc cgtacttgta gaacttgtgt cagaagtggt acacccgctt
     7141 atagttccga tgagtaaagc gcaagttaga atgattgcaa atttcttcaa aaaaattcct
     7201 ccttaaaatt gaatatggac taatactagt cccattgtag cataccgaat ctttaagatt
     7261 aataagaaaa aggactaatt ttagtccaaa aggtgtgcta ttcttgaaag aggaaaaaat
     7321 tattgataaa gaaaatgtaa tcggtagcaa tattagacga gtccgattag aaaaaggaat
     7381 tggtcaaacc gagctgatta gagacctaca gcttcgtaaa attgctataa ctagggaaac
     7441 gttggttaag attgaaggtg gccgccaaca cattaagttg gatcaattga aagccatcaa
     7501 agatattcta caggtgtctt atgaagattt gcttcaatga ctacttgtag tcatttcgtt
     7561 tgaaattatt atgttacacc cttatgtgtc acgaagaaat actggtataa taatgaatgg
     7621 gatatgtaac gatagggtat actgttatgt tgcctaataa attgtggatc aaaatacaag
     7681 aatggaggga tatccttgta taaggcatat gaattggatt ttacaaatgc tgacttaaat
     7741 gcattctcct caagctcgca atcatataca catgtagtaa atcaaataga tcacaaccaa
     7801 aaatctatta acaagagaat agaaaatcta tttgaaagtg aaaatgattt agttgattat
     7861 tattctaagg acagcaaaat tctagatgca gacgaaatta ttaaagattg gtttccaact
     7921 ataaaagcag atatttttat atctcactct catgctgatg aaaaattagc tatacgtttt
     7981 gcatcatggc tatttgaaaa ttttggtctt actgcattta ttgattcttc ggtttgggga
     8041 tacagttctg atttattaaa aaaaatagac caaaaatatt gctataagga acaaacaaaa
     8101 acatatgatt atgataaaag aaatgtaaca actagtcatg ttcatatgat gctttctaca
     8161 gctttaaata atatgattga tagtacagaa tgtctgtttt ttcttaatac acctaattca
     8221 attagtttga gcaatgaaat cactaatgaa cagaaattta cgtattcgcc atggctatat
     8281 agcgaactga cgacagcttc aatagtggaa aaaaagaatc cacgattgga aagtaatcct
     8341 caaatgagca ccgaggatgt tagaagcatt ataaagcact attcttcgca agaaaatctg
     8401 caaataaaat attctttaaa tttaagtgaa ttaacttatt tacgaaaagc tgatttgaaa
     8461 aaatgggctt tagatgctgt ggaatgcact aaaggaagta atacattaga tattttatat
     8521 aaaaacacca ttttcactaa tctctaatca atttactgaa tcgaaaggaa ccgggtagaa
     8581 tggataagaa agttttaata aagaggttct ctgaagctat tgaaaaagga aatgcagcaa
     8641 tatttgcagg tgcaggatta tcgatgtcac aaggatatgt aagttggccg gaactactta
     8701 atgatcctgc aacagaaatc gggttggatt ctaaaaaaga aacagactta gttactctgg
     8761 ctcaatatta taaaaatgaa aatggtggat caagaggaat tttgaatcag atacttatgg
     8821 ataattttgg tgaagaatta gaaatatctg aaaatcatcg aatactagca agtttaccta
     8881 ttgaaactta ttggacaact aattatgatc atctaataga aaaatcaatc cgagaagctt
     8941 ataaaaaccc tcaggttaag aaaaattata ctcagttagc aacaactaat ccaaatgtgg
     9001 atacaattgt ttataaaatg catggtgata tagatgatgt cagttcgaca gtaataacaa
     9061 gagatgatta tgaaaaatat gatgattcgt acgcgttatt taaagaaact ttaaaaggag
     9121 atttgttaac aaaaactttt ctctttttag gatttagttt tacagaccct aatttagaaa
     9181 gaattttatc agatataaga tgggttttga gagaaaacca aagacctcat tattgtataa
     9241 tgagaaaaat attatagaaa aaattttagg ttatcgctga aagccccgga aatacgggca
     9301 tttcgcagga tatgagaact caatttacta ccaatttact actttcggat tgagccgcat
     9361 atatgctgaa ccatactgct aatacccgcc accccaaata cggcacatca taaattgaat
     9421 acgacaccgt aaacgcggcg ttcctctttc ccgtcatggg agaacaggac gccgcttttt
     9481 tatgcccaaa tagagaaagg aggcgacgcc atgctattta cttccggccc tgaccgggcg
     9541 tttgaacggc tgatgcagga aaagcccggt ttcgatcact acgacggcgg cggggcggac
     9601 gcgccggagg attgcggcac ctgccgattt taccgcccgg attggaaata tcaattctgc
     9661 cagtttgcag agtgtcccta tcagccgggc aagctgaccg actatgcagc ggtcaaattc
     9721 acagtgaaag gagcgaacaa cgctatggca gttttccgtg tggaaaagaa caaaggctat
     9781 acggtgatgt caaatcacca cctgcggaat aagaacctga ccttaaaggc caagggcctg
     9841 ctgtcgcaga tgttgtcctt gccggaggat tgggactata ccctaaaagg actgtcctat
     9901 atcaaccggg aaagcatcga cgcgatcagg acagcggtat gggagcttga aaaagctgga
     9961 tatatccggc gtgagcaggg ccgtgacgca aaaggcaaaa tggccgatat ggtctatacc
    10021 atctacgaac agccggtatt ggaaaacccg gtattggaaa atccaacatc ggataacccg
    10081 gtgttggaaa atccgacatc ggataatccg gcgtcggaaa atccaacgca attaaataaa
    10141 gaagtacaaa gaactgactt atcaaaaaaa gaaaaaataa ttacagatgg acaaagtacc
    10201 cattccattc ctatcccatc cccgacccct gcccctatgg agggcgaggc agcggctacg
    10261 ccgccggaac ggaaaaggac aggaaaggat gaggctgtac ggatttatcg ggaaatcatt
    10321 ttggagaaca tcgagtatgc ctatctcatt caggacagga gcattgaccg tgaacagctt
    10381 gacgaaatcg tagacctgat gctggaaacc gtctgcaccg cccgaaagac aatccgtgtc
    10441 gccggggacg actaccctgc cgagctggtg aaatccaagt acatgaagct gaccggcgaa
    10501 catatccgct ttgtccttga ctgtctgcgg gagaacacaa cgaaagtccg caatatcaaa
    10561 caatacctgc gggctatgct gttcaacgcc cccagcacca tttccaacta ctacacggcc
    10621 cttgtcgcgc acgacatggc acagcccgat tggggaaagc ctaaatccgg cctgccggac
    10681 tattcctgcg gcgagggcga aagcctgtaa cgagaaaagg aggacgaccc tatgaagcag
    10741 ggcgcattga tttttgacga gcgaacagac cgttacgaca tccgctttga tctggccgac
    10801 tattacggcg gcctgcattg cggggagtgt ttcgacgtga tgatcggcgg caaatggagg
    10861 cccacccgca ttgaaatggc cgaaaactgg tatcttgtcg gcatccgggc cgacgacctt
    10921 tccggcctgc gggtgcggat ttgacctata tctacctgcg gcgtctggtg gttccgcctt
    10981 agacgccgct tttgactttc tgacaaggag ggatgcgatt gcaggaagaa gttactcaaa
    11041 agaccattgc cctgtccatg aaaacgggca agctcacggc tcaagtgctg caagcggcgc
    11101 tgaaaaagta cctgcaacac cgggccaagg gcaaaaacac gctgcaccac ggccagcaga
    11161 gcctaaagca gcttaaaaag catggggctg atctctccaa cattgagatc accgaggcca
    11221 acattggagc gttcaagcca tgcgccaaga aatacggcgt ggattttacc ttacgaaaag
    11281 ataagaccac ccagccgccc cactacgttg tgattttcaa ggccaaggat gcagataacc
    11341 ttgatctggc gtttcgggag tttaccgcca agacgctttc caaggagcag cggccctcta
    11401 tccgcaaggt tttgtccgct gtgaagcaaa aggcggcggc ccagaccaag cagcgggcca
    11461 aggagaaaat caaggaaagg gggctggaac tgtgaagcct gacgtaaaga aattgctgat
    11521 cctgaacctc ccgtatctga tttttgtgta cctgtttgcg aaatgcgggg aggcgtaccg
    11581 gctggccgct ggtgctgacc tgtcggggaa gctcctgcat tttgcggatg gttttgcggc
    11641 ggcttttgcc aaccctctgc caagcctgca cctgtttgac ctgtgtatcg gtatcgccgg
    11701 tgccgtgatc gtgcggctgg tggtgtactg caagagcaag aacgtgaaga aataccgcaa
    11761 aggcatggag tatggcagcg cccgctgggg gacggccaaa gacatagcgc cttacatcga
    11821 ccccaagttt gaaaataata tcctgctcac ccaaacggaa cggcttacca tgaccggcag
    11881 gcccaaagac cccaagacag cgcggaataa aaacgtgctg gtgatcggtg gcagcggttc
    11941 agggaaaacg agatttttcg tgaaacccaa cctgatgcaa tgctttccga ccacggacta
    12001 tccgacttcg tttgtggtga cagatccgaa aggcactttg gttctggaaa ccggcaggat
    12061 gttccagcag gcgggctacc gcattaaaat cctgaatacc attaactttt ccaagtccat
    12121 gaagtacaac ccctttgtct acatccattc ggaaaaggac gtgctaaagc tggtaaatac
    12181 cctgatttcc aacaccaagg gcgaggggga aaagtcagcg gaggattttt gggtgaagtc
    12241 ggaacggttg ttttattcgg ccctgatcgg ctatatctgg tacgaggccc cagcggagga
    12301 aatgaacttc tcaacgctgc tggaaatgat aaacgccagt gaagcccgcg aggacgaccc
    12361 ggagtttcaa agccccgtcg atctcatgtt tgagcgtttg gagcagaaag accccgacca
    12421 ttttgcggtg cgccagtaca agaaattcct gctgtccgca ggcaagaccc gttcttccat
    12481 cctgatttca tgcggtgcca gacttgcgcc ctttgacatc cgggaggtac gcgagctgat
    12541 ggaggacgac gaaatggagc tggacaccat cggggacgaa aagacggtgc tgtatctcat
    12601 tatgagcgat acggacacga cctttaattt cattctggct atgctccaaa gccagctaat
    12661 caacctgctt tgtgaccggg ctgatgatgt gtacggcggg cggctgcctg tccatgtacg
    12721 cctgattttg gatgaatttg ccaacattgg ccaaataccc aatttcgata aactcattgc
    12781 caccattcga agccgtgaaa tatcggcctc tattattttg cagagccagt cgcagctaaa
    12841 agcgatttac aaggacgccg ccgagatcat ttcggataac tgcgatagtg tgcttttcct
    12901 tagtgggcgc ggcaaaaatg ccaaggagat ttccgatgcg ctggggaaag aaacgattga
    12961 cagtttcaac accagcgaga accggggaag ccaaacgtcc cacggattga actatcaaaa
    13021 attaggaaag gagttgatgt cccaagacga aattgccgtg atggacggcg gcaagtgtat
    13081 cttgcagcta cggggtgtgc gcccgttttt atcggagaaa ttcgatatta ccaagcatcc
    13141 ccggtacaaa taccttgccg acgccgacaa gaagaatacc tttgacgtgg acagattttt
    13201 gactatcacg cgccggaaac ggcagcaagt ggtaacgcag gatgaagtat tcgacctgta
    13261 tgaaatcgac ctgtcggacg aggacgccgc cgctgaataa acagcggctt ttttcattcc
    13321 aacaacaaaa tcaaaaaatc attatgagcc ggaaaccggc agaaagaggt aaatttatgg
    13381 cattttttaa ttccgcagtt actactttgc agactatcgt gatcggactg ggcggcgctc
    13441 tgtgcgtatg gggcggcatc aatctgttgg agggatacgg gcaggacaat ccgggggcaa
    13501 aatcgcaggg tgtcaagcag cttgtcgctg gcggcggcgt ggccctgatc ggtgtcactc
    13561 tggttcccct gctgtccggg ctgctgggct gatcctggcc tttgtccctg tccaaaccct
    13621 taactaatcg cctccgcgtc ctcccggcca gaccgggagg gcgcgggaaa ggaggtgcta
    13681 cccgtgatta gtgaaatcat tgaagaatgg ataaaaggca tcctcatgga cggtatcacg
    13741 ggcaacctgt ccgggctgtt cgatacggta aatgccaaag tcggagaaat cgccgccgac
    13801 gtgggacgaa ccccgcaggc atggaacagc ggtattttca atatgctgca cagcctgtcg
    13861 gaaaccgtga tcgtccccat agccggggcc atactcgccc ttgtcatgtg ctatgagctt
    13921 atccagatga taacggaaaa gaacaatatg cacgactttg acagctccat gttcttccgc
    13981 tggatattca aatccgcgtt tgcaatcctg atcgtaacga acacatggaa catcgtcatg
    14041 ggcgtattcg acgccaccca aagcgtcgtc aatcaaagcg ccggtgtgat tatcgggcag
    14101 acatccattg attttaacac cctgatacca gaccttgaaa gccaattgga ggctatgagc
    14161 gtgggcgctc ttttggggct gtggttccaa acccttgttg tgggcctcac catgaatatc
    14221 ctttccatct gcattttcct tgtgacctat ggccgcatgg tggaaatcta tgttgtcacg
    14281 tcgctggggc ctatccccct tgccacaatg ggcaacgcgg aatggcgcgg catgggccaa
    14341 gggtatctga aatccctgct ggcgctgggc ttccaagcct ttttaatcat ggttgtggtg
    14401 ggtatctacg cggtgctgat acagaatatc ggttccgctt ccgacatatc cggcgcaatc
    14461 tggggctgta tgggctatac ggtgctgctg tgcttctgcc tgttcaagac cggcagtata
    14521 tcaaaggccg tattcggggc gcattgatcc caaaatccaa gcatacacag gaggaacttg
    14581 tttatttcaa cgtacaacat catttacgct gacccgccgt ggcggtatga ctgcaaaaac
    14641 gtacagggcg cagcggaaaa tcactattcc accatgagca ttgacgagct gtgcgccctg
    14701 ccggtggaac ggctggcgtc caaagactgc ctgctgtttt tgtgggccac attccccatg
    14761 ctgccggaag ctctgcggtt aatcaaagcg tggggctttt cattcaagac cgtggctttt
    14821 gtatggctga aacagaaccg taaaagcctt acatggtttt acgggctggg ccgctggaca
    14881 aggggaaacg cggaaatctg cctgctggca acacacggca agccgaagcg tcgttctgcc
    14941 tctgtccatc agtttattat cagcccgatt gaacaacaca gcaaaaagcc ggatgtgacc
    15001 cgtgaaaaaa tcgtagagct ggcgggcgat ttgccccgcg tggagctgtt tgccagacag
    15061 aaaacccccg gctgggacgt gtgggggaac gaggtggaat gtgaccttat cctgacgtga
    15121 aaggaggtgc caaatggctt atgtaaccgt ccccaaggac ttaacccatg tgaaaagcaa
    15181 ggttgtgttt gggcttacca aacggcaact gatctgtttt tccggggcgc tggtgatcgg
    15241 cgtcccctta tattttctca cccgtgacta tcttaccaac agcgcggcgg ccctgctcat
    15301 ggtatttgcc atgctgccgg gctttttgct tgccatgtac gagcgtcacg gccagcccct
    15361 tgaagtggtg attatgcaga tcatccagtg ctgcttcatc cagccaaagg agcgtcccta
    15421 tcagaccgac aacacctaca ccgcccttgt gcggcaatac cagatggaac aggaggtaaa
    15481 ggccattgtc caaaaaggaa atcccaagag aaaccgaaaa aacaaagctg acccgcgccc
    15541 agcgaaaaga aattgacgcg gtaatccgta aatataaggg cgacggcagg ccccacacgg
    15601 cgcagcagag catcccctac gaggtcatgt acccggacgg cgtgtgccgg gtatcgcccg
    15661 gcgtcttttc caagtgcatt gagtttgcgg acatcagcta tcagcttgcc cagccggaca
    15721 cgcagacggc tatctttgaa aaactgtgcg acctgtataa ctatgtggac gcctccattc
    15781 atatccagtt ttctttcctc aatcggaaag ttgacccggt gcagtacgcc aagagctttg
    15841 agatcgcgcc gcagggggac gacttcgacg acatccgggc cgagtacacc ggcattctgc
    15901 aaaagcagct tgcaaacggc aacaacggca tggtaaagac caaatacctg acattcacca
    15961 tcgaggccga aagcgtgaaa gcggcgcgag ccagattgaa gcgcatcggc tttgacctgt
    16021 taggctattt caagagcatg ggggccgtag cccatgtgat ggacggctgg gaacggctga
    16081 acctgctgca tggcgtgtac cacccggacg gcgagatatt caattttgac tggaagtggc
    16141 tggccccgtc cggcctctcc accaaggatt ttattgcccc gtcctccctg tgcttcggca
    16201 atgcaaagac attcggcatg ggcggcaaat acggcgcggt aagtttcctg caaatcctgt
    16261 ccccggagct gtcggacgat atgctggccg acttcctcaa tacggaaagc ggtgttttag
    16321 tcaatctcca tgtgcaggcc attgaacaga ccaaggcgat caagaccatc aagcgcaaaa
    16381 ttaccgacct tgacgctatg aagattgcgg agcagaaaaa ggccgtccgc agcggttacg
    16441 atatggacat tcttcccagc gaccttgcca cctacggcga ggacgccaag aagctgctga
    16501 caaagttgca gacccgcaat gaacggctgt tccagttgac gttccttgtc ctcaacgtgg
    16561 ccgacaccaa gcaaaagctg aataacgacg tgttccaagc cgcaggcgtg gcccaaaagc
    16621 ataactgtcc ccttgtccgg ctggactacc agcaggaaca gggccttgca agcagcctgc
    16681 cgctgggcgt caatcagatc aagatacagc gcagccttac gacttccagc gtggccgtgt
    16741 tcgtcccctt tgtgacgcag gagctttttc agggcggcgc ggccatgtat tacggcatca
    16801 acgccaagtc ccgcaacatg attatgctgg acaggaagca ggcccgttgc cccaatgcct
    16861 tgaagctcgg cacccccggc agcggcaaat ccatgagctg taaatccgag atcgtcagtg
    16921 tgttcttaac gacgcctgac gatattttta tttcagaccc ggaggccgaa tattaccccc
    16981 ttgtcaaacg gctccacgga caggtaatcc ggctgtcgcc taccagcaag gactttgtga
    17041 acccgctgga catcaacctg aattattcgg aggacgataa cccgctggcc ctgaaatccg
    17101 actttgtgct gtcgttctgt gagcttgtca tgggcggcaa gaacgggctg gaagccattg
    17161 aaaagaccgt gatagaccgg gctgtgcgcg tgatctaccg gccctatctg gcagaccccc
    17221 gcccggagaa tatgccgatc ctgtccgacc tccacaaagc gttgttagat cagcatgtcc
    17281 cggaggctga ccgggtggcg caggcccttg acctgtatgt gtccggctcc ctgaacgtat
    17341 ttaaccaccg taccaacgtg gacatcggca accggcttgt gtcctttgac attaaggagc
    17401 tgggcaagca gctcaaaaag ctggggatgc tcattgtcca agaccagata tgggggcgcg
    17461 tcacggccaa ccgaagccaa ggaaaggcca catggtattt tgccgacgag ttccatttgc
    17521 ttttgaaaga ggaacagacg gcggcctatt ctgcggagat ttggaagcgg tttaggaaat
    17581 ggggcggcat tccaacaggt gccacccaga acgtcaaaga cctgctttcc tccccggaaa
    17641 ttgaaaatat ccttgaaaac agcgacttca ttacgctgct caatcaggcg tccggggata
    17701 gaaaaatcct tgcggaacgc ttgaatttgt ccaccgaaca gcagaagtac atcgacaatt
    17761 ccgaacccgg cgagggcctt ttgatctttg aaaacgtggt gctgcccttt accaacccca
    17821 tcccgcacaa cacccagctt tacaagatta tgacgacccg cttaaacgag gtggcgggag
    17881 tatgacccgg cgacgccaca aaggaggtga accaccatga aagagctgaa agccaaggcg
    17941 aaagtgacgc aggccatgac ccgcgacggg ctgacgctgg aaaatcaggc caccggcgag
    18001 gtggagaaca tttccagccg ggaggccgaa caggatttgt ccgcgccggg gccgggcggc
    18061 accgccgata agctgctgga acgggccgac gccatccatg acggccataa ggccaagaaa
    18121 gccgcgaagc aggccggggc tgtggccgcc gagggggacg ctgccctgca ccggccatcc
    18181 gcccggctcc aactgtcgga ggaagaacgg gccgacccgg atttacagcc ctatatccga
    18241 aaatcggaca aacgggccga taagctggat aaggcccgcg ccgccatccc caagaagcgc
    18301 gtcccggtca aggaaaccgt ctttgacgag gcaagcggca aagcaaagac cgtcctgcac
    18361 tttgacaaac aggacaaggg gcctccctcc ctgaaaccca atccggcagg ccgtcctgtt
    18421 tccgaggttt ttctattcgg ccacgggaaa atccatgaag tcgagcatga aaacgtgggt
    18481 gtggagggcg gccataaggg ggaggaactt gccgagcggc aggccggaaa agccatccgc
    18541 agcaccatcc ggcaccacag gctgaaaccc tactgggacg cggaaaaggc cgagcgcagg
    18601 ctattgtctg ccaacgcgga gtatttctac cagaaatctt tgcgggataa cccggagatc
    18661 gcacaggcgg ccagcaaccc catttcccgg ttctggcaga aacagcagat caagcgccag
    18721 tacgccaagg ccgcccggac agcgggccag accgcaggcg cggcggcaca gggcgcggcc
    18781 aagacagccg gggcctccac gaaagcggcc aaggccgcag ccaaagaaag tgaacgggcg
    18841 gcctcctttg tggcccgcca ctggaaaggg gcgctggccg tccttgtgtt ggggcttttg
    18901 atgctgctta tcatcggcgg cttacagtcc tgcaccgcca tgttcggcag cgccggaagc
    18961 ggcatcacgg cgtcctctta cttttccgag gatagcgata tgttggccgc cgaggacgcc
    19021 tacgcggagc tggaagccga gctgcaagac tatctggaca actacgaggc cacccatgac
    19081 tatgacgaat accattttga ccttgacgag atcagccatg acccctatgt gctgatttcc
    19141 attctgtcgg cgctccatga cgggatattt accatcgggg aggtacaggg tgatttgtcc
    19201 atgctctttg acaagcaata tatcctgaca gaaaacgtcg tggtagaaac ccgttaccgc
    19261 accgaaaccg atacatggac ggacgcggag ggcaacaccc atacagacac ctaccaagtg
    19321 ccgtatgact attacatctg ctacgtcacg ctggaaaact tcgacctgtc ccatgtgcca
    19381 gtctatatca tggacgagga aacgctaagt ttctattccc tgtatatgtc cacgctagga
    19441 aaccggcctg atctgttttc cggcagccag taccccaacg cctccaccct gacgccgccg
    19501 acctactacg acgtaccccc ggaggctttg gaggatgcca cctttgccgc catgctcaaa
    19561 gaggcggaaa aatatatcgg ttatccctat gtgtggggcg gcagctctcc cagcaccagc
    19621 tttgattgca gcggcttcat ttcgtgggta atcaatcata gtggatggaa cgtgggacgg
    19681 ctgggggcgc agggcctata taacatctgc acccctgttt ccagttcgca ggccaagccc
    19741 ggcgaccttg tatttttcaa gggaacctac gacacccccg gcgtgtccca cgtcggcctg
    19801 tatgtgggca acaatgtgat gctgcattgc gggaacccga tttcctacac gaaccttact
    19861 tcaaactact ggcaatccca tttttacagt tacgggcgtt taccttaacg ccagaaagga
    19921 gctttcaatt atggcaacca aacttgaccg catcgaaaag gacatccaga aaaccaagtc
    19981 taagatcgcg gagtttcaga aacagcttcg ggagctggaa acgcagaaaa ccgaacagga
    20041 gaatttgcag atcatccagc ttgtgcgcgg catgaacatg aagccggagg aatttgccgc
    20101 gttcttacgc agcggcgcaa tgcaggccgc ccctgccgca accccttacc atgaacagga
    20161 ggacaatgcc catgaagaat aagaccagac ggatttcccg tattctcccc gcacttctgg
    20221 ccgtgatgct ctgcgtcacg gcctttcccg ttaccgccct tgcaggcggc aatgacccgg
    20281 caccggcccc gctgccggag gctacaaccg aacccgctac gggcggtttg gagcctgaaa
    20341 cagacgaaac cggcaccgcc acggacggga cagaaaccga ggccgaaaaa gataccggcc
    20401 ctctgaccgg caaggacatc accgacctgt tttccgcgct gttcggttcc aaggtgagca
    20461 ttgccgccac ggacgacggc atccagatca ccaccggcac cgacaaaaaa gaacctgaac
    20521 agaccggcac cgtcaccacc aacggcggca ggctgaatgt ccgcaccggc gcggggcttg
    20581 acaagaccgc gttcacccag cttcccaatg ggacgacggt tgaggtagtc ggcacagacg
    20641 gcgactggat taaaattctc ttgccggagc gcatcggcta tgtccattcc gactatatga
    20701 ccgtcagcga aaaggaagta gcggctaccg gcgagggcgg tttttccctc tccattgacc
    20761 cggaggaaat tgcttctctg ctggaactgt tcaatggcgg cggggccagc gccgccctgt
    20821 ccccggacgg gaacctgtca ctgattgacg acatcggcag cgccgcgaaa aatggcaagc
    20881 agtttatcac cgtggaaacc aaaaacggca atgtgtttta tctgattatt gaccgggacg
    20941 acgagggaaa agaaactgtc catttcttaa atcaggtgga tgaagccgat ctgctgacgc
    21001 tgatggagga cggggaaacc gtcacccccg ctgctgtctg ctcctgtacc acgaaatgca
    21061 aggccggggc tgtgaacacg aattgcccgg tatgtaaaac caacctgacc gagtgcagcg
    21121 gcccggagcc gcaggaaccc cagccggagg aacccgaagc cccggaggaa gaacccaagg
    21181 gcggcgcggg cggcctgatc gtgttcctgc tggtggcgct ggcgggcggc ggtgcggccc
    21241 tttactattt caagttcaaa aagcccaagg ctgaaaccac cggcagcgac gatttgggcg
    21301 agtatgattt tggggaggac gaggatttgg aggacgagga acccgaaacc gagtttgacc
    21361 cggaagctga ttttgtgagt gaacaggagg acgaggaatg acatataaac ttgtaatcgc
    21421 ggaaaaaccc agcgtggccc gcagcattgc gggcgtgatc ggggccacgg agaaacacga
    21481 cggctatttg gaggggaacg gctatctggt gagctggtgc atcggacatc tggtgtcctt
    21541 tgccgacgcc ggacgctatg acgagcgttt caaaaaatgg cgctatgagg atttgcccat
    21601 tctcccggaa acatggcagt acatcgtccc cgacgacaag aaacagcagt ttgacgttct
    21661 ctgttcgctg atgcagcgcc ccgacgtgac cggccttgtg tgcgccaccg acgccgggcg
    21721 cgagggggaa ttgattttcc gtttcgtcta ccagatggcg ggatgccaaa agccctttga
    21781 gcgcctttgg atttcctcta tggaggacag tgctattaag gacggctttg cccacctgaa
    21841 accgggcggc gactatgatc cgctgtatca atcggccctg tgccgggcca aggccgactg
    21901 gctgatcggc attaacgcca cccggctttt ttccgtcctg taccacaaga acctgactgt
    21961 gggccgcgtc cagacgccga ccttaaaaat gctggtggac agggacgcca agattaccga
    22021 tttcaagaaa gagaaatatc acatcgtcca tatcacggcg ggcggcgcgg acgcggtaag
    22081 cagccgcttt tccgacgctg ccgaggcaaa caccgtcaag gcggcttgtg caaaagcgca
    22141 ggccgtttgc gtttccgtga cgcgggagaa aaagacggaa cagccgccca agctctatga
    22201 ccttaccacc ttgcagcggg aggctaaccg gcttttcggc tataccgcca agcagaccct
    22261 tgactatgcc caggcactgt atctaaagaa gctgctgacc tatccgagaa cggatagtca
    22321 atatcttacc gacgatatgc tgcccgccgt ggaaagcctt gtgtccggcc tgtgggagct
    22381 ggtgccgttt gccaagggcc tgaacctttc cccgcagttt gaccgcatcc tgaacagcaa
    22441 aaaggtgtcc gaccaccacg ccattatccc caccgctgaa tttgtgaagc agggctttgg
    22501 cactctggcc gagagtgaaa aaaagctgct gtccctgatc tgctgcaagc tcttatgtgc
    22561 cgtggccggg ccgcatatct atgaggccgt caccgccaca tttacctgtg ccgggcagga
    22621 gtttaccgcc aagggcaaga ccgtcctttc ccccggctgg aaagagattg aacgccggtt
    22681 ccgttcttcc ttaaagaccg acgccgacga ggacgccgag gcggtgcggg agctgccgga
    22741 gctttgcgag ggccagacct ttgcagacgt ggccgcctct gtcaccgagc atttcaccac
    22801 gccgcccaag ccctacaccg aagatacgct tttgtccgct atggaacggg ccggggccga
    22861 ggatatgccg gaggatgccg agcgcaaggg attaggcaca ccggccaccc gcgccgccat
    22921 tctggaaaag ctggtgcaga tgggctttgt ccagagaaaa ggcaagcagc tcattcccac
    22981 caaggacggc atcaatctgg ctgtggtgct gccggaggcg cttacttctc cccagcttac
    23041 tgccgaatgg gaaaaccggc tgaccgagat cgccaagggg caggccgatc ctgacgaatt
    23101 tatggacggc atcgagacgc aggcgcggga gctggtgcag acctattcct gtatcagtga
    23161 ggaaaagcag aaattgtttc aggccgagcg tgttcctatc gggacgtgtc cccgctgtgg
    23221 tgaggctgtc tatgagggca agaaaaacta ctactgcgga aaccgggcct gccagttcgt
    23281 catgtggaag aatgaccgat tttttgagga acgcaagaaa gcgttcaccc cgaagatcgc
    23341 cgctgcgctg ctcaaaaccg gcaaagtcaa ggtaaagggc ctctattccg taaagaccgg
    23401 caaaacctac gacgggacgg tgcttttggc tgataccggg ggcaagtacg tcaactaccg
    23461 cattgaacac aggagctaaa ggacagtaac acagcaagca tagggagtgt ggctacactc
    23521 ccggtaaaat cccccggccg cgtccctgcc gggccgggga tttttgcttg ttggggaagt
    23581 ccccaacccc ctattttttg aaacagaaca gagaaaggag tgaccgcgtg aacgaagtat
    23641 tttctatcca agtccataac cggcagtttt tggaacaggg caaacaaggc gtctggctgg
    23701 atttgcccac cacaaccgaa acgctgcaag ccgccctgcg ggatgttggc attactgccg
    23761 acaatccgca ggattttttt atcaacgggt attcctgccc ggaggacaga caccttgcca
    23821 ttccctatga catggtgctg gccgccgacg tggacgagct gaatttcctt gccgcccggc
    23881 tgggccagct tgacgccgcc gagatcgcag agctgaacgc cgccctgcaa aatccccgtg
    23941 gcggctttga gaatatcggc cagatcatcg actacaccga aaatgtggat tactatgtgc
    24001 atcttcccga cgttaggggg gatggacagt tgggcgacta ctacctgaac cgttccggca
    24061 tggtggatat gccggaggaa tggaaagccg ggattgatac ggcgcagttt ggacgccaca
    24121 ttgcccagca ggaacaaggc gtttttaccc agtacggcta tctggtgaga agcggcgacg
    24181 aatggcagcg cgtccatgag ggccagccgg tgccggagga ataccgggtg atgcactacc
    24241 cccagccgga agctgaccgg gaggaaccgc aggcccagca gccggaggca tccgaaagcc
    24301 ccgacaaagc gccgcccccg gttatcccga tcatgctgga cggcatgaac agcaaagaac
    24361 gcatgaaagt gattactgac cgtctggaaa ccggcgtaca ggagcttttt gaaagtagcc
    24421 gctatatggc ttacttatcc gctatgtcca agatgcacga ttacagtctg aacaatacgc
    24481 tgctgatcgc catgcagggc ggttcgattg tggcgggcta taacaggtgg caggatgaat
    24541 ttgaccgcca tgtgaagcgg ggcgagaaag gcattaagat actggccccg gccccgttca
    24601 aggttaagca gcagatggag aaaaccgacc cggccaccca gcagcccgtg atcggcgcgg
    24661 acggcaagcc cgtgaccgaa gaacaggaag tgacggttcc ggcctttaag gtggtgacag
    24721 tctttgacat cagccagacc gagggcaagg attttcccat gccctatatc agtgaactta
    24781 ccggcgacgt gcagcagtat caggatttct ttgccgcgct ggaacggaca tcaccctatc
    24841 ccataagcat tgagccgatt tcagacgggg ccaagggccg ctgcttctac gcaaaacagc
    24901 gcattgccat caacgagggc atgagcgaat tgcagaccct aaagaccgcc atccatgaga
    24961 ttgcccatgc caagctccac gccattgacc gggaggcccc cgccgaggaa caggcggccc
    25021 ggccagaccg cagcacccgc gaggtacagg ccgaaagcgt ggcctatacc gtctgccagt
    25081 attacgggct ggacacgtcg gactattctt ttggctatgt ggccgattgg agcagcggca
    25141 gggagttatc cgagctgaaa gcctcccttg aaaccatccg ggccacggcc agcgaattga
    25201 taaccgagat cgacggccat tttgcccagc ttcagcagga gcgcgaggcc cggcaggaac
    25261 aggaggccgt cacggttgac gttaccccgg aggcagcggc cccggcgcag gccgaggaag
    25321 aacaggcccc ggcccttgat ccggcagccg agccgcttgt gactgtgcta tggagcgaaa
    25381 gcccccagct aaaggacggc cagcaaatgc cgttgcatga ggccgacgcg ctgtttgccc
    25441 gtctggatgc agcatatccg gcagaggcag gctatgacaa aaccgcgttc cgcattgact
    25501 gtatctttca gggcgagccg gacagctacg aggggcggca ggattttgga gatagggacg
    25561 gttccctgat cgaacatatc cagagctacc accaatttta cgcgcaggac gaaaactgga
    25621 aaaagcatgt aatcgctaca accggcatag agggctggga gcaggacaag gcccatcggg
    25681 atatgctgtt aggcgagttt gtcccctata tgaagctgca ttgcagcctt gcccaaatgg
    25741 aacagacggc gcagggccgc ttacagtccg gggaaaccct gacgccggag gaaacggcct
    25801 atttgggggc gcttctctct tatgtgcagg agtgccgccc catgctcaat cagggccagt
    25861 atcagttgcc ggagccgccg aaactggctg acttcgacca gagcttgcag gactacaaag
    25921 cacaggtaca ggccgagatc gcgcaggagg ccgccgcagc cggtatgacc gtggaggaat
    25981 acgcggcggc tggctatgaa gcaccccaac aggacacctt ttccatctac cagcttcgag
    26041 gcggggagga aacccgcgac tatcggtttg agccatacga gcgtttacag gccgcagggc
    26101 tggccgtgga cagtgccaac tatgaccttg tttatacggc accgctggat aaaagcacgt
    26161 ctttggagga tatttaccgc acattcaaca tcgaccaccc ggcagatttt aggggacaca
    26221 gcctttccgt ttccgacgtg gtggtgctgc accagaatgg acaggacacc gcccactatg
    26281 tagacagctt cggctatatg gaagtgccgg agtttttgcg ggaaaatccc ctgcgtaccg
    26341 ccgaactgtc tacggagcag aacgaaaaca tgattgacgg cattatcaac aatacgccgc
    26401 ccgaaccgca gccggaggaa cccgaacagc tatcttttga ggatgtggca gttgcttccg
    26461 gccagacaca ggaggaaccc ccggaggctg cgcccccgca aacggctgtc cgctattatc
    26521 ccatcaatga gggagccgcc cgccgcgcca aggaagccat gagcttttct gactaccggc
    26581 ccggcagcgc cacggcggaa taccggcact atgtcgatca ggccgtggaa attgcggagc
    26641 gccagaaaaa gcgtgtggag ccagagtatc acgaaaagat agacagcctc cttgacacct
    26701 acgcccgcaa gctggccgag aacatgaata agggctatga gattaccgcc cgcgtcccct
    26761 ctgtcctgat tgccgggccg tccaatttcc cggtacgggc gaaagaaaag cagaacgccg
    26821 ccagtgaccg caacatggag gaatttcagt acattcaggg cttgcttgac aaaatccgca
    26881 gcaccggcat gggtggtatc agcgccgacg atccgcaggc cgtttccaag ctggaaaaga
    26941 aactggaaaa gctggaagcg tcgcaggagt tgatgaaggc cgtcaatgcc tactaccgca
    27001 agcatgggac gctggacgga tgcccccatc tgatcgaaag aggcattgaa aacctgaaag
    27061 cggatatggc gtcgggctgg cactatgaga aaaaaccgtt ccagtcctgg cagctctcca
    27121 acaataacgc cgagatacgc cggttaaagg gccgcattga ggaattgacc cgccaaaagg
    27181 aggcggccta tgtgggatgg gagtttgacg gcggcactgt ggagatcaac cgggtggcca
    27241 accgcctgca aatcttcttt gaggggaagc cggatgctgc tgtacgcgac gagctgaaaa
    27301 gcaacggttt ccgctggtcg cccaaggccg gggcatggca gcggcagtta aacgacacca
    27361 caatccgcgt cgctgaccgt atcaagtgca tccagcccct ttccggcgaa aagcccagcg
    27421 ccttacagct tgcggcccgc agggagaaag aaaagccctc tatccgggcg cagctcaacg
    27481 cggccaagac cgaacaggcc aagcagccac cggcgcggga aaaatcaaag ggattggagg
    27541 tagagtaaat gcagaagttt gaaaatgtgg acgttctggc cgcgctggaa cagatcatgc
    27601 ggcagaacac cgcgttttat caaagtgact ttggcattga caaagacatt atcaggcggg
    27661 atgccgccag cgatcaggcc gcagacaaga ccctgctctg gatgtcccgg ccctccggga
    27721 cgtactgttt ccgggaacgt gatgttttcc tgaaagacac ccgacagtac aacacatgga
    27781 aattttacgg ggagcagacc cgtgacaaga tactggccta cgcggtggag ctgaccggca
    27841 cacagggcgg cactatccgg ggcaatctct atgagctgga ctatccgcag catttccggc
    27901 acgtcattga aaccgcccag ccggtgagtg tgaaccggct cttttatgag catggcaccc
    27961 gtgacatccc ggagagccag cgttttgacg gaagccctga ccgggtattg ggaaactttt
    28021 tgtactatga ggcccagccc catgacccgg ctgtgctgca ggaggcgcta cggacggagc
    28081 agcacagccg ggggcgggcc gtccccggcg atttcaaagt ccacgtcacc gctttgcatg
    28141 acagccggat tgaaaccgag gcccggcgta ttgtggacag cctgaaagat ctgtccgcgc
    28201 ctaacagccc caataaaacg catttcatgg tggagatctc cccgcacttt gcggcgattg
    28261 cttccaccaa ggataatgac cggctctttg ccatgctgcc ctataaatcc ctgtgtttta
    28321 ccgggatgaa aggcaggcat ggcgtgtatg ccgtgattaa ccgggacgag cgccgggatg
    28381 tgagtattcg caggccccgg ccctctatcc gcaagcagct atccgacagc aaacaggccg
    28441 ccagcccaaa aaaggcggcg gccagaacca aaaagaatga attggaggta taaggaatga
    28501 tgcactttac cgtggaggaa gaaaacctga tctgtatgta ccacaatgca gaccggcgca
    28561 gaacgataaa caggctcacc gccgtcatgc cggttatgga tggggatatg cgggcgcagg
    28621 cccagcagac cctttccaag ctggaacgca tgacggatgc cgactatgac ggccagaagt
    28681 tccacttcac agacgaataa cgaacagcag acgccgggcg cggcggccat gtggtcgctt
    28741 gcgtccggcg tcttttcttt ttgcctattc ctacgcattt agaacccggt attgtcgggg
    28801 ataggatgaa gtattcgctt gcccctgcaa acatgcggga gaagccttgc agcgggcggt
    28861 tttcaatccc ttaaatgcgt aggttgcagc tataattaga cccctgaaac agatatagcc
    28921 gcgcagcggg tgtatctgtt tcaggggtgt gcaggtgata gccgcaaagc ggcgtaagag
    28981 ggtgcagccc tcttgccgtc tatctctgtt ccagcgggaa gctcggcaat gcttccagca
    29041 gtttcaggga aatgcgctgc cgtatatctt cgtcaatgac tttcttcttt ttgccgtttg
    29101 cctgtctgat ctccaccgtg gaaagctcgt tgatgtagtc agcataatgg gccaacactt
    29161 tttccgtggc ccacctctca ccggcaacgg cggctttgat cgtttcatag tccagcagct
    29221 tttcttgtcc gtccatcctg atacccccgt cagactgatt ttttctatga taccatattc
    29281 ggaagcatgg aaccagagaa aacggcgctt ttctgctggt aaattctacg catttagaac
    29341 agcacttcgg cggggatagg ataaaacacc cacatacccc gctgaccatg ccggaaaagc
    29401 cttgaaatat aagggctttt acagctctaa gtgcgtaggt ttccagtatc ttttccgctg
    29461 gcgctcggcc tctttgcggc ggcgcatccg tttggagcag gccgcgcagt atttggcccg
    29521 gttagacttc ggcgtgaacg tggccccgca ctcacagcag cgtttcactt cgtcccggtc
    29581 tttggtgatc tcggcataca gggccgcatc cagcgggagg acagcggcct taaaccagcg
    29641 gcacagaagc gaataggaaa tgctctgtac gcaaatacat tcttcgccgt cgtccagcaa
    29701 caggcagttg ccgtcacaat agttgcagca ctcatgcgtc agcttccggg ccttgcgata
    29761 ttgagggtaa ttcattcgtg gaatactcat agctcttgcg tccattcctt tcccggcgtc
    29821 atacgcaaaa tgccgtccac attctgcttg atgatgccgt attcctgcat ctgctttttc
    29881 gcttcgccgt acttctcata cagccgttcc ttttcctcgg ccaatcgctt gtactcggct
    29941 ttcagggcgt acagatcggg gagctttttc acaccctgtc cctttagggc ctttgccgca
    30001 gcttcataca aaatcaacgt gctttcatgc tcacgccgga attttgcctt atccgctgca
    30061 ttgcggtatt ccatagccac gggccagagc tgcttgtagg tagaaatatt ctttatcagc
    30121 accgccatat ccgcaagccg gtgttccata gctttgaggg tagcggcggc ctcgtcgctg
    30181 gcggcggtga tctcggccac tttgctttcc aaatccgggt aggtgtctat cccatgttca
    30241 gtcaggaaat tcaaagtctt tgcggcctgc ttcaaattgt ggagcttcgc ccacctctca
    30301 tagcctgccg actgctgggc cttaatgttg ttttccagat caatccgcaa agaaatcccc
    30361 ttgcgctctt tgggcgctct ggtgataacg cggcctttga tacgctcttt gatggcttcc
    30421 tcggtgtagg cttcccccaa tgtcttgcag cgggtaaagc gttcctgtcc gggagcgcgg
    30481 aaagaaataa actttcccgg cttgatctca tagccctgcg cctgcatgag ccgcaggaaa
    30541 tcatcaaaat cttttgcctg cgggatagcg gcgtcaatgg ctgctttgag cttcgctttc
    30601 cagctcgtcc ctttccgctg ggcgtcccac tcggcatagc tcttgccctt actctggccg
    30661 ggcttcacca cggacaatcc gttttccttg cacagcctgt cgctggtgcg ccggatatag
    30721 gcatagctct gtttgttgga aatgtacttg cgctgattta ccatatccac ggcacagaaa
    30781 ataatgtgat tgtggacgtg gcccttatca atatgggtag tcagcacata gggatatttc
    30841 ccttgcagca cttcctcggc aagctgcttc ccgatctcat gggcctgttc cggcgtggct
    30901 tcgccggggg caaaggattg tatcaggtgg tggcccagat tattgccctt ttggtatgcc
    30961 tgcgccagca gcatttcaaa ttcaatgtcc gctgtttcat aagagcaggc aaaggaggcc
    31021 accagcagct tgccgtcagt tttgtccggg ttctcgatgt agtcaagggc ttttttcagc
    31081 gttgacttga taggatgtat ctttgtaact gccatatctg cgccagcgcc ccctttatat
    31141 cctcgatgtc ctgtgcgtac accggcccca taccgttcag cctctttgca atctgattga
    31201 cgttgacgcc gatcttctgt atctcggcgg tctgcgcccg gatgtcgctg gtgtccatgt
    31261 tgacgatata gccgtcaatg agcatcttcc gggcgtaggc tgaaaagttc ttcgtatgca
    31321 gcagcgccat tttctgttca atcatagccc gttcctccgg cgtcacaggg acgcgcagca
    31381 cgatttttcg ctttctgttt gccatgcttc accgtccttt ccattcaggg gtttggggat
    31441 ttccccaaca agcaaaacgg acgcggcccg gcagggacgg ggcaggccgt ttttccggga
    31501 gtggctacac tccctatgct tgctacgtta ctgtttctcc actaacaaga cgtttccaaa
    31561 gccccaaact acccgcaaaa tagccctctg gaaagaaaaa agtgcaaaaa aatagaagct
    31621 acgctgtgca gctcccgcta aaagtaaaac agaggatgcc agttacacat cctccgtttc
    31681 attcttcttc ggcttttcaa ttagtatggc gctgtcaatc gcgccctcga tgatcggcag
    31741 gtattcttcg gggcagagtt tcagcttgtg gctgaccctt tgccgcagtt cgctttcctc
    31801 ccgcatgatc tccgggttaa agtagcgttc cacaggaagc ccgcaggctt taataagctg
    31861 tatgacaacc ggcagactgg gaatggcccg tcgttttcga tgttggcaag atagcgccag
    31921 tcaatattta ttttctctgc cattgctttg cgcgaaacgc gctttgcttg ccgggccgct
    31981 ttcacatccg cgccgaaagt ttcaaagccg ggacaatctt caatattcgc catatcgcac
    32041 ctcccactta cattgtatat ttcatactct gcccgtggaa tgttggtata tgcacctttc
    32101 ttaaagttta tatttcacat tttgtttctt ttattcttcg agcaaaagaa ttataatgta
    32161 ccctgtggag gtgtactatg gactacatga cgttgaaaga ggcaggcgag aaatggggtg
    32221 tgcccccacg atggataaac tactattgtg ctgcgggacg catccccggc gctgtaaaga
    32281 tggcgacgat ctggctcatt cccaaaactg cggaaaagcc gattgacagg cgtacaaaga
    32341 aaggaaaggc ggttactgaa tagtataatg aaaccggcgc attttggata tggttttgaa
    32401 gagagagaaa gcatggtatt ttgtcacgga aaaactataa gatgatagat ggcaggctgc
    32461 tccagaccga taaaaagtat tctcatctga aacagaaaga aaagatttac acatggatgt
    32521 atgaagaagc gaaactttat catgacagaa atggaaaatg cccgaaacaa agacgaggaa
    32581 gctgctgctg tatcagccgt atatggtaga attgaaaagg ccggcatctg gattctttat
    32641 ggtgaagtct ccagacattg gataactgct ggtctaatgc gcctttgagt atggggtgat
    32701 tgattttaat accggggagg tacggctttt tgaggaaaat attctgggat ttacatttgg
    32761 cgattattac atttcctgta actgcaagga cacggatgat gaagatatga ggaatataga
    32821 cttgctgatt ttctactgtc ttgaacggta ttaggactga atctgctgcg cggagtgcgg
    32881 caagagcgac cggagggagt acacacaggc cctgcctgtg tgaaaagcca taccgcaagg
    32941 tgcattcgca aaaaagaggc cgcgcatttt gctgaggctt ggggtgtccc caacaagcaa
    33001 aacgagaacg ccgcaggcgg gcaagtggtg gatcagaata tccgtaaagg atattctgat
    33061 ccattgcttg ccagtattat cgggatgttt tgacaaccgc tgtaatcgaa acatcccaaa
    33121 cattaagaag gtgttaatta ttattatctt gatttaattc gtttaaacca ataatataga
    33181 tacatacacc atgaaaagaa gcgagtatcc caatgacgga tgattaaaat ccgaaaagaa
    33241 atcgctttga aactacaggg aaactacaga ctgttatgtt atcttcttaa aatggaggga
    33301 tttttatgtc gatacgaatt ctacttgtcg aggatgatga tcatatctgt aatacagtaa
    33361 gggcgtttct ggctgaggca ggatatcagg tggatgcctg cacagatgga aatgaggcat
    33421 acaccaagtt ttacgaaaac acttatcaac tggttattct tgatattatg ctgcccggta
    33481 tgaacgggca tgaacttttg cgtgaatttc gtgcgaaaaa tgatactccc attctgatga
    33541 tgacagccct gtcggatgac gaaaaccaaa tccgggcgtt tgatgcagag gcagacgact
    33601 atgtaacaaa gccattcaag atgcagattt tactaaagcg ggtggaagcc ctgctgcggc
    33661 gcagcggtgc gctggcaaag gaaatccgtg tcggcaggct gacacttctg ccggaggatt
    33721 tcacggtact ttgtgacggt acagagctgc ccctgacacg aaaagaattt gaaatccttt
    33781 tgctgctggt gcagaacaaa ggcagaacct taacgcatga aatcattttg tcacgtatat
    33841 ggggatatga ctttgagggt gatggcagca cagtccacac tcatatcaaa aatttgcggg
    33901 ccaagctgcc ggaaaatatc atcaaaacca ttcgcggtgt aggataccga ttggaggaat
    33961 cataataatg gaaagaaaag ggattttcat taaggttttt tcctatacga tcattgtcct
    34021 gctactgctt gtcggtgtaa cagcaacact gtttgcacag caatttgtgt cttatttcag
    34081 agtgatggaa ttacagcaaa cagtaaaatc ctatcagcca ttggtggaac tgattcagaa
    34141 tagcgatagg cttgatattc aagaggtggc agggctgttt cactacaata accaatcctt
    34201 tgagttttat attgaagata aagagggaag cgtactctat gccacaccaa atgccaatac
    34261 atcaaatagt tttagaccca actttcttta tgtggtacat agagatgata atatttcgat
    34321 tgttgctcaa agcaaggcag gtgtgggatt gctttatcaa gggctgacaa ttcggggaat
    34381 tgttatgatt gcgataatgg ttgtattcag ccttttatgc gcgtatatct ttgcgcggca
    34441 aatgacaacg ccgatcaaag ccttagcgga cagtgcgaat aaaatggcaa acctgaaaga
    34501 tgtaccgccg ccgctggagc gaaaggatga gcttggcgca ctggctcatg atatgcattc
    34561 catgtatgtc aggctgaaag aaaccatcgc aaggctggag gatgaaatcg caagggaaca
    34621 tgagttggag gaaacacagc gatatttctt tgcggcagct tctcatgagc taaaaacgcc
    34681 catcgcggct acaagcgttc tgttggaggg aatgcttgaa aatatcggtg actacaaaga
    34741 tcattctaag tatctgcgcg aatgcatcaa aatgatggat aggcagggca aaatcatttc
    34801 cgaaatactg gagcttgtca gcctgaatga tgggagaatc gtacccatag ctgaaccgtt
    34861 ggacataggg cgcacggttg ccgagttgct gcccgatttt caaaccttgg cagaggcaaa
    34921 caaccagcgg ttcgtcacag atattccagc cgggcaaatt gtcctgtccg atccgaggct
    34981 gctccaaaag gcactatcca atgtcatatt gaatgcagtt cagaacacgc cgcagggagg
    35041 cgaggtacgg atatggagtg agcctggtgc tgaaaaatgc cgcctttttg ttttgaacat
    35101 gggcgttcac attgatgata ctgcgcttcc aaggctgttc accccattct atcgcattga
    35161 tcaggcgcga agcagaaaaa gtgggcgaag cagaaaaagt gggcgaagcg gtttaggact
    35221 tgccatcgta caaaaaacgc tggatgccat gagcctccag tatgcgctgg aaaacacctc
    35281 ggatggcgtt ttgttctggc tggatttacc gctcacatca acattgtaaa tatttaaaac
    35341 ttaaatgatt ttgggctctt tgtcaaatag gactgataga gtgagataag aacatctaag
    35401 gtagaattcc ttttctactt tagatgtttt ttgattcata ggtaatcaga ttatttagta
    35461 agaaaattct ggtcttcatt ttccgaaagc ttcgaaagcc ataagcgact cgtttcaatg
    35521 ctttgatgtg ggtatgcaga ttttctattt tggcatttga ataaggcaat tcgagggcta
    35581 aacgaatgcc ggattcatag tttataaaag ctttttttat agaatgcagt tcttttggaa
    35641 cggaattggg catcgttctt acaagtttaa agaaagtgtc cgcctctttg tcgtgaaacg
    35701 cttgaagaat ttcttgatag taaaaatagg cgattcgtaa ttcgtcagag atcgaaagca
    35761 gacgatccac cacttcactt tcggtgagat acgcccaacc aaaattgcgc catttggtcc
    35821 atttgtcata aacaagcttg tcctgtcgtt tgatcagaag ccgagacaac gcctttaatt
    35881 gtcgataata tttggcttgt gttggatcgt ttcgatcaaa gcttttcatg attcgtacgc
    35941 gaatatcctc aaatttacga atgagatgtt tgacaatgtg aaaacgatcc acaacgagtt
    36001 ttgcctttgg aaaacattcc tttatcagag aggcataaga aacattcatg tccgtcacga
    36061 tgacctttac cttcaatcgt gcgcgtcgtt caaattgcat aaaataagaa acgagagcct
    36121 ttttcttccg tgaaggaaga atatccagta attttccagt atcgccatcc atacaagaaa
    36181 aggagtaggg accgacttga tttttcagtg aacgaaattc atccattaat aaacaacgcg
    36241 gtaaccaatt tctttttact tttgtcatgg ggatcagaga agcgagtgtc cgagaaacgg
    36301 tcgaccaaga aacgccgcaa tgcttagcaa ttgtcgtcat tgcttgtttt tcagttagct
    36361 cttctaaaat tttatagcgc acttgtttag cgataaagca gtttctatcg acaatgtagg
    36421 tattcgccgt aaaataggtg tggcagccac gacagtggta gcgttgtttc gctaaagcaa
    36481 gataagtcag ttggttcgca catttattaa gttgaatgag cgattttttc tttccgttct
    36541 taacgatcat gccttcatga ttcatctgtt tacacaaagg acagcgacga atttctttac
    36601 ttaacgtcgc agaaacaaca agactcttga tattcttaat gctcttcgat tccacatttg
    36661 tgatgaaaat gtttttatct ttgattccaa gtagtagttt gatatgatga agatcaggca
    36721 tacagaacgc tcctttgatt gtgttgtggt gattcaattc taacggagct tctgtatgtt
    36781 ttgttttttc atgctcaaaa gggaccgata gaatgatttt ctatcagtcc tagaaattat
    36841 agagccgatt ttgaccgaca ggtataaccc tgccggtctt tttttgctgc tacaggaaaa
    36901 ctacagactg gctacaggga aagtacagat gcgcttgcca taataacaat cgtaccagcc
    36961 acaaatcgta gttttattgc aaaggaggca ttcgatcaaa tggaaaaaag caactatcat
    37021 tccaatgcgg atcatcacaa acggcatatg aaacaatccg tggaaaagcg ggcttttcta
    37081 tgtgcgttca ttatttcgtt cacagtctgc acgctgtttt tggggtggag actggcttcc
    37141 gtattggagg caacacagat accgcccatc cctgcaactc atacaggcag cagcactgac
    37201 gtagtggaga atttggagga aaacgctctt gccaccgcca aagaacaggg agatgaacag
    37261 gaatggagcc tgattttagt gaacaggcag aaccccatcc ccgcacagta cgatgtggag
    37321 cttgagcaac tatcaaatgg tgagcggata gatattcgga tttctcccta tcttcaagat
    37381 ttgtttgatg ccgcaagaac tgatggagtt tacccgattg tcgcatccgg ataccgaaca
    37441 acagaaaaac agcaagaaat tatggatgaa aaaattgccg aatataaggc gaaaggctac
    37501 acctctgcac aggctaaagc ggaagcagaa acttgggtgg ccgtgccggg aacgagcgag
    37561 catcagcttg gtcttgctgt ggatatcaat gcggacggaa ttcattcaac aggcaacgag
    37621 gtttatagat ggctggatga aaacagctat cgttttggtt ttattcgtcg ctacccacca
    37681 gacaagacag agataaccgg tgtgagcaac gagccgtggc attaccgata tgttggcatc
    37741 gaagctgcca cagagatgta caaccaaggg gtttgccttg aggaatattt aaaaccagaa
    37801 aaatgagaaa aggatgttat gttatggaca gaaaaagatt gacacagcgt ttcccgttcc
    37861 tgcttccaat gagacgggcg cagagaaaaa tgtgctttta tgcgggaatg agatttgacg
    37921 gctgtcgcta tgcgcagacg ataggggaaa aatcgctttc ccatttgctc tttgaaacgg
    37981 attgcgcatt atacaaccac aataccggat ttgacatgat ataccaggaa aacaaggtgt
    38041 tcaacttaaa gctggcagca aagaccttaa acggcttatt gataagaccg ggggaaacct
    38101 tttctttctg gttgttcctg catacgccat tgacaattat ccagcgcaga ggtcacgaag
    38161 taaaggagtt tccagagcca aacagtgacg agattaaagg ggtggatgca accatttcag
    38221 agggctggat tgatttaaaa gtgcgaaacg ataccgactg cacctaccaa atatgggtga
    38281 ccttagatga tgagaaaatc atcggtcagg tgttcgccga caaagagccc caagcattat
    38341 acaaaattac aaatggcagt atccagtatg tccgtgaaag tggcgggatt tatgaatatg
    38401 cccaggttaa acggatgcaa gttgccttag gtaccgggga aataatagat tgcaagctgc
    38461 tttatacaaa caaatgcaaa atctgttatc ccctcccgga aagtgtggat attcaggagg
    38521 agaaccaatg agaaaaagtg tgggcattac tgtttttgga tgcgaacagg atgaggcaaa
    38581 tgctttccgc gctttatcgc cggattttca tattatccct acgctgatta gcgatgcgat
    38641 atcggcagac aacgcaaaat tggccgctgg caatcaatgc gttagcgtaa gccataagtc
    38701 cgaggtttcc gaggcgacaa ttcttgcgct gagaaaggtc ggggtaaaat acatttctac
    38761 ccgcagcatc ggctgcgatc acattgatac gactgccgcc gagagaatgg gaatctcggt
    38821 tggcacggtt gcgtattcgc cggacagcgt tgcggattat gctttgatgc tgatgctgat
    38881 ggccatacgg ggtgcaaaac ccaccatgca cgccgtggcg caacaagatt tcagattgga
    38941 tcgtatccgg gggaaagaac tgggggatat gactgtggga gttattggaa ccggccatat
    39001 cgggcaagcg gtcgtcaaaa ggctgcgggg atttggatgc catgtgctgg cctatgataa
    39061 cagccgaaaa atggatgcag attatgtcca gcttgatgag cttctaaaaa acagcgatat
    39121 tgttacgctc catgtgccgc tttgtgcgga tacccgccat ctgatcggtc agaagcaaat
    39181 tggagagatg aagcaaggcg catttttaat caacaccggg cgcggagcgc ttgtcgatac
    39241 cggggcgctg gtggaggcgt tggaaagcgg aaagctgggt ggtgcggcac tggatgtgtt
    39301 ggagggcgag gatcagtttg tttataccga ttgctcgcag aaagtgcttg accatccctt
    39361 tttgtcgcag cttttaagga tgccgaatgt gatcatcaca ccccatacgg cgtactacac
    39421 tgagcgtgtg ctgcaggata ctacagaaaa aacaatcagg aattgtctca attttgaaag
    39481 gagtttacag catgaataga ataaaagtcg caatcatctt cggcggttgc tcggaggaac
    39541 atgatgtgtc ggtaaaagcc gcaatagaaa ttgctgcgaa cattgatacg gaaaaattcg
    39601 atccgcacta catcggaatt acaaaaaacg gtgtatggaa gctatgcaag aagccatgta
    39661 cggaatggga agccgacagt ctccccgcca tactctcccc ggataggaaa acgcatgggc
    39721 tgcttgtcat gaaagaaagc gaatacgaaa cacggcgtat tgatgtggct ttcccggttt
    39781 tgcatggcaa atgcggggag gatggtgcga tacaggggct gtttgtattg tctggtatcc
    39841 cctatgtggg ctgtgatatt caaagctccg cagcttgcat ggacaaatca ctggcctaca
    39901 ttcttacaaa aaatgcgggc atcgccgttc ccgaatttca aatgattgat aaaggtgaca
    39961 agccggaggc gggtgcgctt acctaccctg tctttgtgaa gccggcacgg tcaggttcgt
    40021 cctttggcgt aaccaaagta aacggtacgg aagaacttaa cgctgcgata gaagcggcag
    40081 gacaatatga tggaaaaatc ttaattgagc aagcgatttc gggctgtgag gtcgggtgtg
    40141 cggtcatggg gaacgaggat gatttgattg tcggcgaagt ggatcaaatc cggctgagcc
    40201 acggtatctt ccgcatccat caggaaaacg agccggaaaa aggctcagaa aatgcgatga
    40261 ttacagttcc cgcagacatt ccggtcgagg aacgaaatcg ggtgcaggaa acggcaaaga
    40321 aagtatatcg ggtgcttgga tgcagagggc ttgcccgtgt tgatcttttt ttgcaggagg
    40381 atggcggcat cgttctaaat gaggtcaata ccctgcccgg ttttacatcg tacagccgct
    40441 acccacgtat gatggccgcc gcaggaatca cgcttcctgc actgattgac agcctgatta
    40501 cattggcgtt aaagaggtga taagtatgga aaatggtttt ttgtttttag atgaaatgtt
    40561 gcatggcgtc cgttgggatg ccaagtacgc cacatgggat aacttcacag gaaaaccggt
    40621 ggatgggtat gaggtgaatc gcatcatcgg cacaaaggcc gtggcgtttg ctctgcgcga
    40681 ggcacaaatc catgcggctg cgcttggcta tggcttgctt ttatgggatg gatatcggcc
    40741 aagaactgcg gtggactgct tcctgcgttg ggcagcgcaa ccggaggaca atctcacaaa
    40801 agaaaaattt taccccaata tagagcgagc cgagttgatt acaaagggtt atgtggcctc
    40861 acaatccagc catagccgtg gaagcgcaat tgatcttacg ctctaccacc tggatacagg
    40921 ggaacttgtt tcaatgggaa gtaacttcga ttttatggac gaacggtcgc atcatacagc
    40981 aaaagggata ggggatgcag aggcacaaaa tcgaagatgc ttgcgtaaaa tcatggaaag
    41041 cagcggattt cagtcttatc gctttgaatg gtggcactat aagttgattg atgagccata
    41101 ccccgatacc tattttaatt ttgccgtttc ataatgaaag tattggtttt gtatcagatt
    41161 ttggatttga taaaatctat gctggtcatg gacaatcttt tactggaaag tggttaaagc
    41221 atcttttgta atacattgca aattatagta ttcaatctgc ccagcggcag aaaagaaaaa
    41281 aaccgctgtt gggcagacct ttcccacgcc cattttgtat tttgctggcg gcggttttga
    41341 gaaatcaagg ccgccgttct tttctggcag caaaaggtgt ggcggcccat aggaggatgc
    41401 cgctatgctg ttttgctatt gccatgctct aatccacttc caccactcaa aaaaagcctt
    41461 gcccctc
//
LOCUS       DAZAID010000015        39802 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000015 DAZAID010000000
VERSION     DAZAID010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.015"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..891
                     /locus_tag="ACG9BE_000734"
     CDS             22..891
                     /locus_tag="ACG9BE_000734"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFN3031510.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1056..2327
                     /locus_tag="ACG9BE_000735"
     CDS             1056..2327
                     /locus_tag="ACG9BE_000735"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFN3031511.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2324..3619
                     /gene="rho"
                     /locus_tag="ACG9BE_000736"
     CDS             2324..3619
                     /gene="rho"
                     /locus_tag="ACG9BE_000736"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFN3031512.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3718..3981
                     /locus_tag="ACG9BE_000737"
     CDS             3718..3981
                     /locus_tag="ACG9BE_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFN3031513.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4426..4875
                     /locus_tag="ACG9BE_000738"
     CDS             4426..4875
                     /locus_tag="ACG9BE_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3031514.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            4932..6350
                     /locus_tag="ACG9BE_000739"
     CDS             4932..6350
                     /locus_tag="ACG9BE_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN3031515.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6350..7210
                     /locus_tag="ACG9BE_000740"
     CDS             6350..7210
                     /locus_tag="ACG9BE_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFN3031516.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7384..8157
                     /locus_tag="ACG9BE_000741"
     CDS             7384..8157
                     /locus_tag="ACG9BE_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN3031517.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8304..8669
                     /locus_tag="ACG9BE_000742"
     CDS             8304..8669
                     /locus_tag="ACG9BE_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFN3031518.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8817..10025)
                     /locus_tag="ACG9BE_000743"
     CDS             complement(8817..10025)
                     /locus_tag="ACG9BE_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3031519.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10044..10322)
                     /locus_tag="ACG9BE_000744"
     CDS             complement(10044..10322)
                     /locus_tag="ACG9BE_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031520.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10515..11171
                     /locus_tag="ACG9BE_000745"
     CDS             10515..11171
                     /locus_tag="ACG9BE_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN3031521.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(11613..12410)
                     /locus_tag="ACG9BE_000746"
     CDS             complement(11613..12410)
                     /locus_tag="ACG9BE_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFN3031522.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12407..13591)
                     /locus_tag="ACG9BE_000747"
     CDS             complement(12407..13591)
                     /locus_tag="ACG9BE_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFN3031523.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13616..14863)
                     /locus_tag="ACG9BE_000748"
     CDS             complement(13616..14863)
                     /locus_tag="ACG9BE_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFN3031524.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15078..16823
                     /gene="dnaX"
                     /locus_tag="ACG9BE_000749"
     CDS             15078..16823
                     /gene="dnaX"
                     /locus_tag="ACG9BE_000749"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFN3031525.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16843..17157
                     /locus_tag="ACG9BE_000750"
     CDS             16843..17157
                     /locus_tag="ACG9BE_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFN3031526.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17182..17778
                     /gene="recR"
                     /locus_tag="ACG9BE_000751"
     CDS             17182..17778
                     /gene="recR"
                     /locus_tag="ACG9BE_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFN3031527.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17842..18486
                     /gene="tmk"
                     /locus_tag="ACG9BE_000752"
     CDS             17842..18486
                     /gene="tmk"
                     /locus_tag="ACG9BE_000752"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFN3031528.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18501..18830
                     /locus_tag="ACG9BE_000753"
     CDS             18501..18830
                     /locus_tag="ACG9BE_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFN3031529.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18844..19782
                     /gene="holB"
                     /locus_tag="ACG9BE_000754"
     CDS             18844..19782
                     /gene="holB"
                     /locus_tag="ACG9BE_000754"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFN3031530.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19818..20642
                     /locus_tag="ACG9BE_000755"
     CDS             19818..20642
                     /locus_tag="ACG9BE_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFN3031531.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20635..20982
                     /locus_tag="ACG9BE_000756"
     CDS             20635..20982
                     /locus_tag="ACG9BE_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFN3031532.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21051..21923
                     /gene="rsmI"
                     /locus_tag="ACG9BE_000757"
     CDS             21051..21923
                     /gene="rsmI"
                     /locus_tag="ACG9BE_000757"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFN3031533.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22032..23153
                     /gene="dinB"
                     /locus_tag="ACG9BE_000758"
     CDS             22032..23153
                     /gene="dinB"
                     /locus_tag="ACG9BE_000758"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFN3031534.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23207..23809)
                     /gene="nrdG"
                     /locus_tag="ACG9BE_000759"
     CDS             complement(23207..23809)
                     /gene="nrdG"
                     /locus_tag="ACG9BE_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFN3031535.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24124..26313)
                     /gene="nrdD"
                     /locus_tag="ACG9BE_000760"
     CDS             complement(24124..26313)
                     /gene="nrdD"
                     /locus_tag="ACG9BE_000760"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFN3031536.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            26639..27406
                     /locus_tag="ACG9BE_000761"
     CDS             26639..27406
                     /locus_tag="ACG9BE_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3031537.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            27396..29438
                     /locus_tag="ACG9BE_000762"
     CDS             27396..29438
                     /locus_tag="ACG9BE_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3031538.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            29719..29868
                     /locus_tag="ACG9BE_000763"
     CDS             29719..29868
                     /locus_tag="ACG9BE_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFN3031539.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            29879..31393
                     /gene="dltA"
                     /locus_tag="ACG9BE_000764"
     CDS             29879..31393
                     /gene="dltA"
                     /locus_tag="ACG9BE_000764"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFN3031540.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            31390..32595
                     /gene="dltB"
                     /locus_tag="ACG9BE_000765"
     CDS             31390..32595
                     /gene="dltB"
                     /locus_tag="ACG9BE_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFN3031541.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            32640..32873
                     /gene="dltC"
                     /locus_tag="ACG9BE_000766"
     CDS             32640..32873
                     /gene="dltC"
                     /locus_tag="ACG9BE_000766"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFN3031542.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            32876..34144
                     /gene="dltD"
                     /locus_tag="ACG9BE_000767"
     CDS             32876..34144
                     /gene="dltD"
                     /locus_tag="ACG9BE_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFN3031543.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            34285..35367
                     /locus_tag="ACG9BE_000768"
     CDS             34285..35367
                     /locus_tag="ACG9BE_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFN3031544.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            35367..36170
                     /locus_tag="ACG9BE_000769"
     CDS             35367..36170
                     /locus_tag="ACG9BE_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFN3031545.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            36240..36980
                     /locus_tag="ACG9BE_000770"
     CDS             36240..36980
                     /locus_tag="ACG9BE_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN3031546.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            36991..37995
                     /locus_tag="ACG9BE_000771"
     CDS             36991..37995
                     /locus_tag="ACG9BE_000771"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFN3031547.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(38040..>39802)
                     /locus_tag="ACG9BE_000772"
     CDS             complement(38040..>39802)
                     /locus_tag="ACG9BE_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031548.1"
                     /translation="LFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDSK
                     YTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESN
                     PGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVL
                     TMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPF
                     VHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELP
                     ELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIY
                     QTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHI
                     QEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLIL
                     ARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIY
                     LSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQE
                     KDTFIDRITVQLSRNYPNPV"
BASE COUNT        12763 a         7013 c         8264 g        11762 t
ORIGIN      
        1 aaacttagga ggaattttat tatgccagta gtatcaggag ctgaattttt aaaagctgct
       61 cgtaaaggcg gttacgctgt cggcggattc aacacaaaca acttggaatg gactcaagca
      121 atccttgaag cggctgaagc aaaacaagca ccagtactta tccaaacatc aatgggtgca
      181 gctaaataca tgggtggtta caaaatctgt aaagatttaa tcactgattt agttgaagca
      241 atgaacatca ctgttccagt tgctatccac ttagaccatg gtgattacga agctgctttg
      301 gaatgtatcg aattaggcta tacttcagta atgttcgatg gttcacatct accatttgaa
      361 gaaaacttga aattagctaa agacgttgtt gaaaaagcac atgcaaaagg tatctcagtt
      421 gaatgtgaag ttggttcaat cggcggagaa gaagacggaa tcatcggtaa cggcgaatta
      481 gctgatattg aagaatgcaa acaaatggtt gctacaggta tcgactactt agcttgcggt
      541 atcggtaaca tccatggtca atatccagaa aactggaaag gattagcttt tgaccactta
      601 caagcaattg ctgatgctgt aggtccagac atgcctttag tattacatgg cggatcaggt
      661 attcctcaag atcaaatcga aaaagctatc tcaatgggta tctcaaaagt aaatgttaac
      721 actgaattcc aattatcatt tgctgcagct actcgtaaat atatcgaagc tggtaaagat
      781 aaagaaggta aaggattcga ccctcgtaaa ttattagctc caggtaaagc agcaatcgtt
      841 gaagatgcta aatctcatat cgactggttc ggttcagcta acaaagcgta agcatccaca
      901 taacagatac gatatatagg aatggagaac gacttttgaa gtcgctctcc attctttttt
      961 acttaaaacg gcagaccatg ctcgtttctt ttttttatct atgataaaat acaagcatat
     1021 ccatgaaaaa agattaataa acaaggtgga aacagatgaa aaaaatcgta atcaaaggaa
     1081 accgaccttt agctggtgaa gtaacgatca gcggtgcaaa aaatagtgca gtcgcattga
     1141 ttccagctgc aatcttagct gattccccag tgatcttgga aggtgtgccg gacatacaag
     1201 atgttcattc attaattgaa attttagaga tcatgggcgc aactgtccat ttttccaaca
     1261 acatactaga aatcgatcct cgagaagtcg tatcgattcc gatgccgcac ggaaaaatca
     1321 atagtttgcg tgcttcttat tattttatgg gaacactgct tggtaaattt ggcgaagcgg
     1381 tagtaggcct gccaggtggc tgttatttag gccctagacc gattgaccta cacgtcaaag
     1441 gatttgaagc tctaggtgct gaagtgacaa atgaacatgg tgccatgtat ttacggacag
     1501 aaaataaagg attgcgtggc aatcggattt tcatggacgt ggtttcagtc ggagcaacga
     1561 tcaatgtgat gttagctgct gtaaaagcaa aaggacagac agtaattgaa aacgctgcac
     1621 gagaaccgga aatcatagat gtggcaactt tactgaataa tatgggggca aaagttcgag
     1681 gagcgggtac agatgtgatc cgtatcgaag gagtcgaaac attacatggc tgtcggcatt
     1741 tcatgattcc tgaccgcatc gaagcaggaa cctatctagc attagcagct gcagtaggaa
     1801 atggtatcaa agtgaaaaat gtgatctttg aacatttgga aagctttata gcaaaattgc
     1861 aggaaattgg tgtccatatg aaaatcagcg aagatgaaat cgaagtttac ccctcgaaag
     1921 aattaaaacc cgcaaacatc atgacctatc cttatccagg atttgccaca gatctgcagc
     1981 agccacttac tgcgttacta ttgatgacta caggcacttc agaaatcatc gatacgatct
     2041 atgcgaaacg tgtgaaccat gtgccggaac tagcacgaat gggcgctgat attaccgtgg
     2101 aaggaaatat gatcatcgtg aatggtccaa ataaacttca cggcacagaa gtcgtggctt
     2161 ctgatcttcg ggcaggtgct tgtttagtga ttgctggact attggcagaa gggacaacga
     2221 cgatttacaa tgtagattat atcttacgcg gttatgatca tattatcgag aaactaacag
     2281 ccttaggggc atcaattgaa atgatagagg aagtgggagc tgaatgagcg attatttgac
     2341 aatggcagaa ctagagaaca aaacgttaaa agaagtttat agttatgcca aagaatttaa
     2401 aattccttat tacagtaaga tgaataaaaa ggaattatct ctagctgtga ttcgtgcaca
     2461 agcagagaaa caaggatttt attatatgga aggaatattg gatatcgttg gtcaagatgg
     2521 ttacggtttt ttacggccaa tcaattatgg accaagtgca gaagatatct atatttctgc
     2581 ctcacaaatc agacgattca gtttgagaaa tggggacaaa gtggcaggaa aagcccgtcc
     2641 acctaaagaa tcggaacgtt attatggttt gatgcacgtg gaaagcgtta atggaaaaga
     2701 tccggaagaa gcaaaagaac gtccgcattt tccagcctta acacctcttt atccgcaaag
     2761 acagcttcaa ctggaaacaa caccgcaaag actatctacc cgaatgatcg atatcttttc
     2821 tccaatcggt tttggtcagc gtggattgat cgtcgcaccg ccaaaagcag ggaaaacttc
     2881 tgtattaaaa gaaatcgcaa atggtatcac cgaaaactat ccggaagtag aattaatcgt
     2941 tctactgatt gatgaacgac cggaagaagt aacggatcta gaacgaagcg tgaaaggcga
     3001 cgtggtatct tctacatttg atctgcagcc gcaaaaccat actcgagttt ctgaattagt
     3061 attggaacgc gcaatgcgtt tagtcgaaga taaacgagat gtcgttatct tgatggatag
     3121 tattactaga cttgctcggg cctataactt agtcgttcca ccaagcggac gtacattgag
     3181 tggcgggatt gacccagccg cattgtataa acctaagaaa tttttcgggg cagcaagaaa
     3241 catcgaagaa ggtggcagtt tgacgatttt agcgaccgct ttagtcgata caggaagccg
     3301 tatggatgac gtgatctatg aagaattcaa aggaactgga aatctagaat tacaactttc
     3361 acgtgaatta tctgaacgtc ggatcttccc agccatcgat atcaaaaaat caggtacccg
     3421 aaaagaagaa ttattgatgc caagtgaaga attagaagaa atttggaaac ttcgaaaaaa
     3481 tatgactgga gattcattag aatatacgga acaactgatc cgctttttgc gtaaaacaaa
     3541 aaacaatcag caatttttca aagaatttca agatgtctcc tttggtaaaa aaaatccgac
     3601 cagaaatctg aaaagataat tgcaaccttg actgaaacat gttaagatgt taatgttgta
     3661 aatcgacaaa tcaactctgg ttcagcaaat ggagcagggc aaaggagcga aaaaatcatg
     3721 aaagaaaaca tccatccaga ttaccaccca gtagtattca tggattcaac aactggtttc
     3781 aaattcttgt caggttcaac aaaaacttca caagaaacag ttgaatggga agacggtaat
     3841 acatacccag tcatccgtgt cgaagtaact tctgactcac atccattcta cactggacgt
     3901 caaaaattca cacaagcaga cggacgtgtg gaccgtttca acaaaaaata cggtctcaaa
     3961 gacgcaaacg ctgcggaata ataaaaacgc tgataactca acgtttagca gactatctga
     4021 tagggatagt ctgttttttt atgttttttg ccaatttaaa acttcgtttg atgctgaaaa
     4081 agtcgtagga tcataatgca caaaacaatg aaaaactagt atatagtttt aattatccac
     4141 ttgataaaaa caccttatat taacgaaatg aagaggggtt atttcgaagt aaaaaaccat
     4201 ttacctaaaa ttagaagcta agctttcctt tcaaggaagg ctttttgttt tgttatagac
     4261 ttgcgattgt ttgtgagcta gatcgttcat tttaatgact tatacacgcg gatgatgggt
     4321 ctttttcctc tgttcattca taatattgaa cggtacagct agcgatgcta tactgacctc
     4381 agttccaaga aaacgttaac attttttgtg ggggagaggt tttttatgag gtttgatcaa
     4441 ttgatcgcag aagaactgat tgttttcgat ccatcgatag attcaaaagg atcattattt
     4501 gagcgaatag gggatgtgtt gaaaaaaaca aataaggtaa gaaatgttca aaaagtcgtt
     4561 aaggcactat tcaaacgaga ggaggagatc tccacaggaa tagaagatgg atttgggatt
     4621 ccccatgcaa aaagcaaata tgtgacaaaa ccagcactgt gtttttttca tacagccaaa
     4681 ttaacggact atagcgggat agatgaaatg ccaattgaat atatttttgc catcgtagtt
     4741 cctgaaaaat cagcagatat gcatctagag attctcagca cactctccag acgattgatg
     4801 aatgcagatt ttagaaaggg aataaaagaa gccactacgc cagcagatat catcagacag
     4861 attagtcaag aatgaaaata gtagagtctt tgtccgagta tcgatagtga agaaaaagga
     4921 ggaaaataat catgaaaatc gttggtgtaa ctgcttgccc gactggaatt gctcatactt
     4981 atatgtcggc agaaaaatta gaagtaacag caagaaatct aaaccatcaa gcaaaattcg
     5041 aaacacaagg tgtaaaaaca gaaaatcagt tatctgaaca agaaatcaaa gaggcagatg
     5101 caatcattct tgcagtagat aaagaaatcg aattggaccg atttgccggg aaaaaagtaa
     5161 agcgtgtctc tacaagccga gcaatcaaag aaccgcaagt agtaatagac gaagcattga
     5221 gagatattgg cgtattcgtt gtgtctaatg aaaaagaacc agctgcaaat gagaaaaagc
     5281 cgacaatcta taatcatttc atgaatggtg tgaattacat gcttccattt gtgatcgcag
     5341 gcggaatcat catcgcactg agttttgcct ttgggattac agcggctgat cccaactcag
     5401 cagactataa tgtgttagca gctgcatttt caaggatcgg aggagataca gcgtttgcca
     5461 tgatggttcc tgcgttaggc gcggggattg ctacatcgat tgctggaaaa gcaggttttg
     5521 ccccaggaat tgttgccgga ttactggcct ctacaggtgg ttcaggtttt cttggcggaa
     5581 tgattggtgg cgtgctagca gggtatatct gtgattttct ggcaaataaa attagcgtaa
     5641 aaaaagaatt ttctgctatt tatcagctga ttgtcgtccc ctttttatcg atcttaatta
     5701 ttgggctttt gatggtattt gtgattgaac aaccgatcac atgggtgttg gatgctttga
     5761 caaattggct gaatagcttg ggaaatacat ccgggctatt gttcggatta cttatcggga
     5821 tcatgatggc agctgatatg ggtgggccga tcaataaatc gacttcgact ttctcgatcg
     5881 gtttgatgtc agctggagta aatgcaccga ttgctgcttg tatggctgca ggaatgacac
     5941 ctcccctagg tattgcttta gccactttat tattcaaaca taaatttacc aaagaagaac
     6001 ggacttcagg tcaatcatgc tggattttag gcgcttcgta tattacagaa ggtgctatac
     6061 cgtttgcggt atctgacccg atccgcgtga tacctagttt gatgacaggt tcagcagtcg
     6121 ctgcagggat ttccatggca gtaggagtta cttcaatggc acctcatgga gggatttggg
     6181 tgatgctaat tccaaatgtt atgaacaatt tacctatgta tgtcttggca attgcatcag
     6241 gtacagtcac tacagccttt atgcttggac tattgaaaaa aacagtgaaa gagaaaaaac
     6301 aagacaagtg gacgaaaaat gaaaatctaa caaatgaggt ggaagcgtaa tgttatatac
     6361 aatgaaagag ttattgaaag tggctaaaga gaatcaattt gcagtaccag ccttcaatat
     6421 ttgcagctat gatatgatga aagcaatcat cgaagaagca gagagattag aagcaccagt
     6481 aatcttagaa attcatcctg atgaaatcgc ctatttaagt gatgagtttg ttgcggcagt
     6541 aagagcctat gcttatacaa gtaaactgcc gatcgttatc cacttggatc atggcggtac
     6601 gatagaagat gtcttacggg cgattcgaaa cggctattct tccgtgatga tcgatgcgtc
     6661 tttgaaatca ttcgacgaca atgtacagat tacgaaaaaa gtggttgcgt tagctcatgc
     6721 ggtaaatgtc tcagtggagg ctgagcttgg aacgattggg aataatggtt ctgctgaagg
     6781 aggtgctgaa acaatcattt atacagatcc tgatcaagcg aagatttttg tgaaagaaac
     6841 aggaatcgat acacttgctg tagcaattgg aacagcgcat ggcttgtatc cgaaaaacag
     6901 aactccaaaa ttaaatatcg acttactgaa agaattgaac caacaaatcg atcttccatt
     6961 tgttttacat ggcggttcag gtaatccgga cgcagaagtc agcgaagcag taaaatacgg
     7021 agttgccaaa gtgaatttga gctcagatct taaaagcgtc tttttcaatg ctttgagaga
     7081 aatattgagt gaaaatccat cgatgtacga gccgaatgca atttatccag cagctaataa
     7141 aaaggtacaa gaagtggtca aacataaaat ggaaattttg aacacagtag gcaaggctag
     7201 tttatactaa aaataaaaat gtgtggtcaa gagggtatga caatagtctt gtcataccct
     7261 cttaaccaca cacgatgttc agaagtagtt ttttcgaaaa atttcttctt atttctcgaa
     7321 gccaatcact tctgtcacaa cctctttttt cctgttacta tataaagtaa atggggtgat
     7381 tttatgttga atgaagtgga gaatagacag caggagattg tgaattatct aaaaaacaac
     7441 caatttgcaa gagtcaccga tttgatcgag ttagtaaatt atagcgaagc cacgattaaa
     7501 cgtgaccttg tttttctgga aaaaagtgga ttgatccgta gagttcgcgg tggcgccatg
     7561 cttattgaca atcgaaaaat tgacgtgcct tacctgatga agatcaccaa attagaagaa
     7621 gaaacagaaa aacgtaagat tgcagataca gcttcttctt tgattcgaga tgatatggtc
     7681 ttattcttgg attccagtac aacctcttta cacttagtca gaaacctagc aagatttgat
     7741 ggtttgcaga tcattacgaa tggcatcgtc acagctgcta tgttgagtga atatacaacg
     7801 gcaaaagtat ctgtattggg aggggcaatt gttccgaaac gagcaacaat caacggttca
     7861 aaagcctata acgatgcatt gtcatattat gcggatttag cgataatgag ttgtcgcggg
     7921 ctagatttca gccaaggagt aacagaaact catgaaggag aggcaatggt caaaaaagct
     7981 tttagaaaac aatcagaccg gcttgccgtt ttagttacac agcaaaaaat tgcacataaa
     8041 ttcatgcatc aagccattgc ttttcatgaa atcgattatc tggtgacaag taaagaactg
     8101 acaaaagaac agtatgcgaa agtaaaggaa caccatatcg aatgtctgta cggataaatt
     8161 attacgtgaa tgttctaagt ggcttcctca tttttagata ggaccttatc tttgctcgct
     8221 tctctcagcc gatggtataa taatgaaaag gttatcttgt tagataatct tactttttta
     8281 tccaggagaa aggagtgcag aaaatgaaac atgtgaaggc gttggtagtg aaggcaatca
     8341 tgatctgggc gattctgtgg gtcgtgctga caggtttata cggtgttagc tttatggatt
     8401 caacgattgt tggggtaatt attgtagtca tgatctatgt gctaggggat ctgttgatct
     8461 tacgtaaagt agggaacatc gcagcgacga tcgcagatgc tggttcagct gcagttatct
     8521 tgtggctcta tctatatttt atgaatgata cagtcgatat ttggatgaaa gtattgattc
     8581 ctgctctatt gatcggtgtg gcagaatggt tcttccacaa gtggttgcta agtcaaggca
     8641 tcgtaccaga tgaacggaaa atggaataaa tcaacagtta aactattctt atcgataaat
     8701 gaaagcttta aaaataaata gatgtttaat taagaataat cataaactat ttttactcaa
     8761 aaaaccgctc agattatttt cagatctgaa cggttttttt tatctattca tcaagatcaa
     8821 atttcatttt tcaatgattc aatcaattcg ccctctgctt catgactatg ttttcggcca
     8881 ttaacaagat agagtgctgt acatatcaat ggaatgactc cggcaatcac atacaatcct
     8941 ctaaatgaag taagagattt tagcatcccc atcaaataag gaccaacgcc taagcctagg
     9001 tctaaaccaa taaagtaggt agataaagca atactgattc gatgttcgcg gacgattttt
     9061 aaacaaacag cctgtccatt tgacataaac gtaccataac ctaggccgac taatgctcca
     9121 gagataagca atgtcacact attcgtcgtt acactcagta aaaataatcc tgctgttaag
     9181 aataaatagc taggatacat cacgtattgt tctcctttga catcgaaaat ccttccagtc
     9241 aatggacgtg taaatgtgat gaccaaagca tatacgacga agaaaaatga cccagcagca
     9301 actaagtgga ttacttttgt ataagaagat aaaaatgata aaacacttga ataagaaaga
     9361 cccatcaaaa aggcaattcc tgtaatgaaa agtgctttct tttcaataaa actattgata
     9421 ttccatgagc gaagagatat tttttgttct tcagataatg gaatattttt tacaggaaag
     9481 acaaggcagg caatcgtaac aaagaagacc agtacaattg aaaaccagat aatgaaacga
     9541 aaatcagtca tattcagcaa aatcattcct aaaaatggcc caacagcggc agctaaacta
     9601 gtacttaatc cataataatt gatgccttcc ccttttttag atgcagggat atatgcagta
     9661 acgatagcat ttgtagctgt agaaactgta ccgtagccga atccattcaa gaatcgaact
     9721 aaatacaatg ccccgatagt tggcgtataa agatatgcta atgttgaaag gagatagaag
     9781 atcgcgccgc ctcttaaggt aaacttccga ccaaatagtt ctaactgttt ccccatgaat
     9841 aagcgtgcca gcaatgttcc gatgatatag atcccagaag caaaacctgc ttggctcaag
     9901 ctggcgtgta aggtgtcttg cgcaatgact gccatgatga ccatcaacag ataataaatc
     9961 aggtagacaa taaaattgat caatgtgatt ccaataaaat atttattaaa taatttcttt
    10021 tccatgtatg ttatacattc cccttaagct gaatatgctt ttaatttttg tatttgaact
    10081 tctacatcgt gataattgga tggatagcga tcaacaagtt ggcgaataaa taaatctctt
    10141 gtagacataa acagatttaa aaattcttcg cattcgtttg ttggattctc atccactgct
    10201 ttgatcaatt cgatcgagtt ttgatacgca tcatttaaaa agactaaaga agctttcata
    10261 tcaaatcttg tttgatatgc tgtttctaat cgattaatga ttgtgttcat ccagtaattc
    10321 atgttttttc cctctattct taaaaaattt tacttctgca gtataacgaa taaaaaaaag
    10381 gaaaaaaatc gcataagtga tggaaaaagg tacttaacaa atgttaatat cattagtaaa
    10441 atacctttgg actttaaaaa gttttaaagt actgtatgat ctttttagat gaaagtttgg
    10501 atgggggagc atcattggat agtagagtct taaaggaata tttggataaa aatgattttc
    10561 cgattatacg caaaggatat cataaatatt tgacattcga agggatggaa gattcccata
    10621 cgtatatctt gaaagaggga acggtaaaag caagtgttat ttcaaatgat gggagagaat
    10681 ttaaccttcg ctatatcaaa gatcttgaaa tcgtctctct tttaaaagac gaatattcac
    10741 aattcatcga ttcgccgtat aatattcgag ttgaatcaga atatgcagag ttttatcgaa
    10801 tcaatcgtgt gcgcttttgg caaaatatca accaagaccc aaagctacaa cactatgtaa
    10861 aagagtatta ccgctatcgt ctgctttatt cgatgaaaaa aatgcagcaa atgctattaa
    10921 acggaaagct gggagctgtt tgtacgcaac tttacgaatt atatgattta ttcggtgtgc
    10981 ggacagaaga agggatgctg atcgattttt cgatcacgaa tgaagaaatc gccaaatttt
    11041 gtggcatctc aactgctagc agtgtaaaca gaattttaaa acaactgaaa aaagatggta
    11101 ttatcaccgt taaagaccat catatcgtta ttttgaatat ggacttgtta gaagacaata
    11161 ttatcttttg atgaacaaag aggttgtgaa atcagcgttt tgacctgagt attagagaaa
    11221 caaacggaaa aatagttcct catattttgc cgttttttga gctaataccg agggtcagct
    11281 gattgaacac cgtttaccaa gaggttgtga gcctgccgtt taactctgag tcacaaggaa
    11341 caatttcaaa aaacagcttt tcatgtttga tgaatattgt gacttgtgac cgaggagttg
    11401 gtaggagaac accgtttacc aagaggttgt gaaaaagctg ttctgcatca agtaataaga
    11461 agagccaagt aaaaacagtc tcttatgttt ttaacttggc tggacttatc accgtagatg
    11521 taagcttttg aacgccgttt atttgcaaca aaaggggctg tgacacgatt tttgtcacag
    11581 cccttttttt gcgaacattc atttagcaat ttttagaaac ttctgccgcc accgccaaaa
    11641 gtcccgccac cgctagaatg ggtagtactt ccgccaccgc cgcttccagg aggtgggctt
    11701 ttggggattc ttcttgtcgt gacaaaagaa tttgtcagac gatcggtttt ttcagttaat
    11761 ttgatactgg atttttctcg gaatggatat ttgtagatgc ctgatttcaa ttgataacgc
    11821 gaaacagtca caaaataaaa gacaagacta aggataagag ccaaaacaac agcaatgatg
    11881 atttctactg tcgtcaaaac cttatagcgt gtaattttgc cagtttcttc gtctacacga
    11941 taatgtcctc ctggaactcc gtcttccaca tatgttgctg atttagacag gtaagctttt
    12001 gcagcattgt aataggcagc atttgtcatc tgatcataaa catcatctaa gatcgaatcg
    12061 attctgctat cagtcaaata atcgatcata ttaccagaag tcgaaatgta gatttcacgc
    12121 tgtcccatat ctaatagcaa tactgagcca ttttgatcat ttccgactgc gtctcttaag
    12181 taattatcag caaagtctct tggttcttct gaattagatg tagtcgttac gatcaagact
    12241 cgtcctttga ttttttcatt cagcttttct gcctgttgtt ccaaagattg gatttgatct
    12301 gttgtgaata acccagcctc atcatttacc gtagctgtat ctgcatgagt agccaccggc
    12361 cagccaacac ttactagcaa aagaaagaat aaaaaaaatt ttttcatcat atcaagtaac
    12421 ctccgatcag aaagaggatc gctagcccag cgaaaatccc aagagcggtc agtcccaatt
    12481 ttttctggct gatcggcagg acaccgctga cctttcctgt ttgtccattc attgcgtagt
    12541 agtagacttt tttacttgag tcattgcttc gataagtaac aagccatacc ggcagtaata
    12601 cgtaattatt tacttcgttt gtgattgaag cagaagttcg cacaccggtc aatgttgtat
    12661 atccatttgc ggtatctttt aacagcttct ctgaatagtc ttttaattca gattgaatct
    12721 gtgatttgat tgcctcatat tcgatatctc ttttttctgc ttggaacccg gctaaatact
    12781 ggcttttaaa atcaattgct ttatctaacg ggaaaggttg cacgccttcg accatctttt
    12841 gttgcgtatt ttttgataag gcatttttta ctagttcttt aaaagaaagc ttgcctgctc
    12901 ttgaaacagc aaattgcttc gtttctgtgt attcgatgtc acctatccgc catatgcgga
    12961 taactgtacc attcgcctgc atggcaccat ctacttcagc atctgtagcc caataaggga
    13021 aatacacacc tgtcaactta tcgatttgat ccttattgaa aaaagctttg ggaacaaacc
    13081 attttttctt ggtccaagcg agaaatctgt tgattgcttc ttctttctcg acagcaaaag
    13141 gaagtacttt atttggcaag aattctccgc ttaaacgtcc cgatagtacg acgggattat
    13201 ggcagtagta acaataagtc gcagcagttg tcgcatcagt cacgatctct gcaccacagt
    13261 tgggacaaag aaaaagatct atcgctgcgg tattatctgc agattcgtcc gctgtatctg
    13321 aaaaaccgcc tgcctcgtca accgctttgc gctcggcttc attcatatcg gcaagctgtt
    13381 cttgggctgt aaaagtaaaa tcttcggtag gtgaaggttc ttctttgctt tcttcgccag
    13441 ccatacgcgc ttctttttgc ttttgctcat attggctgac ttcttcttct gtatagatat
    13501 tcaggcagta ttcgcaatga aatttttggt ctttgggatc aaaagtcaac ggaccgccgc
    13561 aattaggaca tttatgtgta agaacatcca tcagctgcac acctttctaa ataaattaat
    13621 taacggggat tcctttaaac ggtttaccac agtttggaca gaatttaggg atgccgttgg
    13681 aaagatccac gatctcattg cattcactgc atcgcatttg tagcttaggc tgaacgtttt
    13741 ctacaggttt ggcagctccg cagttggaac agaattttcc ggaattttct gtcccgcaaa
    13801 ctggacatgt ccaagtatcc gcatttcctt gtgcagtttg ttgattttgt ttttctgctt
    13861 gttgtttgat ttgttcttgg ttattttgag ctgcttgaga aagataagaa ccatttgcat
    13921 tcattcccat acctacaccc ataaagccgg tcattgctcc tgcttcattt tttccggctg
    13981 cttccatacc acgtgcaatg gaaccttgca cgtagccttc acgtacgcta ggatcaccta
    14041 gcatcgcccc ttcgttacgc atattgatca gtttcacaga atcatcggtg taagaaatgc
    14101 tggctacagc cacagaaaca atctccatcc cacgtaattc acgccaagaa tcatccaatg
    14161 cagtatccat gtatttgctt aattccaaac tcttagatgg tacataagaa atccgttgac
    14221 catctgcaga catctgattg attgctgatt gtagcgctgt taggaattca gctaaatact
    14281 gctcattgat atcattgatt tccacttgtg ttttgttttt agggattgcg tttgtataaa
    14341 ataaaatcgg gtcaacgaca tgaatagaat aagtgccatg tgcccgtaag aataattctg
    14401 cgttatagaa attgtcaaag tagttcaacg gcgcgcttgt accgaatttg attcccttta
    14461 tttcttggag attgatataa aaaacttgtt gtttctgcgg tgtgacgccg ccaaatttga
    14521 atcggtcaaa agtttcagca attgcttctt tcagtgttcc attgaacatg gaaggagctg
    14581 cgtcattttt aatggtgtaa taaccttcct cagcggtata atcgatgatt tttccaccat
    14641 cgaccaaaag catcatcatg tttggataga catggacgac tgttccgtct gttaaaacat
    14701 cttctgttcc tttacgattg gagccacgtt tgtcatcttt gcggactttg acaccttttg
    14761 tcatgaccgt tgtatcactc atattgtctg gttcgatgac ttctagccat tgatctgcca
    14821 gaccgccgcc aaccatactt gttgctgctt tgataagacc cataaaaaat tcctcctctg
    14881 attcagctag tgccaaacaa ttattgttta tttattatac cataggaaga tgtgaaactt
    14941 ctcggactag agacggaatt gaaatggaaa attgcgtatt gtttaaatac ctgaaaaagc
    15001 cgagaaggat agtagttttt ttacctaata tcacgtataa taagagatag tgaatttttt
    15061 aaaaaagggg gatccttatg gcatatcaag cactttatcg agtctggcgt tcgcagcgtt
    15121 tcgaagacat cgttggacaa aaagctgtga cacagacact aaaaaatgct attgtttccc
    15181 acaaaacctc acatgcctac ttgtttacag gacccagagg aaccggaaag acgagtgcag
    15241 caaagatttt cgcaaaagca atcaactgtc cgaacagtaa agacggtgag ccatgcaacg
    15301 aatgcgaaat gtgtcgatca attactgctg gtacgcaaga agatgtgatc gaaatcgatg
    15361 ctgccagcaa taacggagtc gaagaaatcc gatttatccg tgatcgggca aattatgcgc
    15421 ctacaaaggc agcgtacaaa gtgtacatta ttgatgaggt acatatgctt tcaacaggag
    15481 ctttcaatgc cttgttgaaa acattggaag agccaaaaga aagtgtcata ttcatcttag
    15541 caacgaccga acctcacaaa ataccagcta cgatcatttc acggactcag cgatttgatt
    15601 ttaaacggat caatacggca gatattgtcg aacatcttga atttatcttg aaagaatcga
    15661 ctatcagcta tgaaccagct gcattgaatg tgattgcccg ttctgcagaa ggcgggatgc
    15721 gggatgcatt gagtattctt gatcaggcaa tctcgtttag tgaaggaaca attactttag
    15781 atgatgccat gcaagtgaca ggtagcttaa ctgacgaaat gatggacagt tacttgagtg
    15841 cttgcgtgaa aaaagaagta ccagaagcat tggaaacatt gaaccaaatt ctcgcagccg
    15901 gcaaagaatc acgtcgcttt ctagaggata tgttacaata ctgccgtgac ttgctgatgt
    15961 atcagcaagc acctaaccta gttgaggaac aatcgagtac gatcagaagt acatttacag
    16021 aattggcaca aactgtttca gcggatcagc tgttttcaat gataaagatt ttgagcgata
    16081 ctcaaaatga aattcgtttt accaatagtg ccgctattta tttggaagtc gctacagtca
    16141 aacttgcaca gctaccgtct gcttctgttc cttcagcaga tgtagcattt gcagatgatc
    16201 agatagtaaa acagctacaa caagagatta aggaactaag aaacgaaatc aaggaactac
    16261 gcagtaatac aggaattgag aatacaaaag tacagccggt acagcaaaca aatcataaaa
    16321 aacagacaag tacctatcgt gtaccgacgg aacgagtata taaagttctg cgggaagcga
    16381 caagacagca tctgaaccaa gtaaaagaag tatgggaaga cttgctgatg agtctatctg
    16441 tcactcagcg agcaatgctt aaagcaagcg aaccagttgc agctggacct acaggactag
    16501 tgattgcgtt tgattatgag attgtttgtc aaagagcagc aaatgacgaa gaattgcagc
    16561 tttcggtaca caacaactta agccgaatga tcaaagatta tgcgcctgat cctgtttata
    16621 ttacaagaga aagctggcca gaacttcgaa gaaattattt gacgcaggca caagaagagg
    16681 gaacagaaaa ctcttttgct tctgaagact cagaaggaga aatcgagttg cttcctcaag
    16741 aacaagaagt accagaagaa acacttgtag taaaagcaga ggaactgttt ggctccgaag
    16801 ctgttacagt ggtagatgat taataaaaag gagtagataa ttatgatgcg tggaatgggc
    16861 aatatgcaag gcatgatgaa acaagtacaa aaaatgcaaa aagaaatggc agaagcacaa
    16921 gagctgctaa ataaaacaga gtttacaggt aaagccacca atgaattggt caaagcggtt
    16981 tttacaggcg atcgtcgcat gaaagatatc caaatcaatg aagaagtcgt tgatcctgat
    17041 gatatcgaga tgcttcagga tcttgtcgta atggcagtca atgatgcgat cgcacaaatt
    17101 gatgtggaaa ctgagaaaaa attagggaaa tacgcaaaag gtttgccagg attttagttt
    17161 taaactgaag gagtaatgaa tatgcaatat ccagaaccaa ttgcgaaact gatcgacagc
    17221 tacatgaaac ttccaggaat cggtcagaaa acagcgacaa gactcgcctt ttatacgatc
    17281 gatatgaaag atgaagtagt aaatgaattt gctaaatctt tgttaagtgt aaaacgcgat
    17341 ttgcattttt gtagtatctg cggaaacatc acagaagaag atccctgcga aatatgtaga
    17401 aatccttcac gtgatcgaag cgttatccta gtggtcgaag agccgaaaga tgtaatggca
    17461 atggaaaaaa tgcgagaata tcacggactt tatcatgttc tccatggtgt gttatcaccg
    17521 atggaaggga caggtccaga agacatcaat atttcttctc tgttgcagcg gctgcatgat
    17581 gcagatgtaa aagaagtgat tatcgcaaca aatgcaacaa cagagggaga agcaacagct
    17641 atgtatctct cacgtctgat caaacccgca ggtatcaaag tgactcgtct agcacatggt
    17701 ctgtctgtag gctctgatat cgagtatgca gatgaagtta ctttattgaa agctgtcgaa
    17761 ggaagacgtg agttataaat ttctaattaa gtgttttttc agatacaata taaaagcagc
    17821 acaagagatg ggggcaacaa tgtgaacgga ctatttatta caatcgaggg gcctgatggt
    17881 gcaggaaaaa ccagcatctt gaatgaactt taccctcttt tgaaagaagt agcaaaaaca
    17941 gaaatcattc aaacaagaga accaggaggc attccgattg ctgaagagat ccgagcagtg
    18001 atccttgatc caaaaaatga tcggatggat gaacgtacgg aagcactgct ttatgcagca
    18061 gcaagaagac aacatctagt cgaaaaggtt ctgcctgctc tggcaaaagg gaaaatcgtt
    18121 ttatgcgata gatttgtcga cagttctctt gcttatcaag gtgcaggccg acgcatcggc
    18181 gttaccgaga ttgctcggtt gaatgagttt gctacagaag gtactacgcc ggattttacc
    18241 ctttatcttg acgtagattc agacaccgga ttaagaagaa tcaagaaaaa cagacaaaat
    18301 caaatcgacc gtttagattc ggaaggtttg gaattccacc agcgtgtacg tcatgcttac
    18361 ttaaagctgg cagaagagaa tccggaaaga atccacaaag ttgatgcaag aaaaagcttt
    18421 gaagaagtgc tgcaaacaag ttatcataca attattgaac agtatccgca attttttgaa
    18481 aactaggaag gtgaacgttt atgaaaatta ttttagcgat tatccaagac aaagacagca
    18541 accgtttatc aaatgaattg attgatgcaa atatccgtgc cacaaaacta tcatcgacag
    18601 gcggcttcct taaggctgga aacagtacgt ttatcgtggg tattgaagat gaccgtgtag
    18661 aagaagcatt ggaaatcatc aaaaaaacgt gtgaatcaag aaagcaattt gtatctacac
    18721 ctgttacact ggatatttcg atggatgggg gcgtccctta tccagtagaa gtagaagtag
    18781 gcggtgccac agtcttcgtc ttacctgttg aaggattcca tcaattttag gaggagctga
    18841 gctatgaatc aagaaataaa cttggctcaa atgcaaccga tcgtgtatca gcagcttcaa
    18901 cggagttttg agcatgggcg tatcgctcat gcttatcttt ttgaagggga gaaaggaaca
    18961 ggaaaacatg aagtaggtat atggcttgca cagcacctgt tctgtaccca aatgaaagac
    19021 aatctgcctt gtggaaaatg caacaattgt cagcggatcg aaaagaaaga acatccagat
    19081 gttttggtta ttgaaccgga aggacaaacc atcaaagtcg atcagatcag acgtttgcag
    19141 acggaattca gtcgcagcgg ttacgaatca agaaaaaaag tttttttgat caaagaagct
    19201 gaaaaaatga atagcagtgc ggcaaacagt ttgttgaaat tcttggaaga gccccctgga
    19261 gattttttag cggtgttaga aacagatgca gtaggaagaa ttctccctac tattcaatct
    19321 cgttgtcaag ttctgcattt tcaagaactt tcgaaagaag cactgatcca tcgattgcaa
    19381 gaagagcaga ttccattgga aaaagcaaaa cttttggctt tcttaacaaa cagtttagga
    19441 aaagcagttg aaatctctca agatgaatgg tttaatgatg ctaaggactt gatttatcaa
    19501 tggttcgttt acttgcaaaa gcaagatact caagccttta tctatgtcca aaaaaaattg
    19561 gtaaaatcat ttaaagaaaa aacacaacag tttacaggat tatctgtctt gatgttttat
    19621 tatcaggaag cacagcgtca agcagtagca aacggcgaat atggcaaagt gaaacagata
    19681 aaccagataa ttgaacggat attgatcgca gagcagaaac tacgcagcaa tgtcagtttt
    19741 caggctgttg cggaacagtt cgttttgcat acgatttttt agcttgccgc aaaggatgat
    19801 gatcaaggag gatggaaatg gtagaagtag taggtgtccg atatagagat gcaggtcata
    19861 tctattattt tgcaccagga gactctgaat atgtttatag tgacaaggta ttagtcgaat
    19921 ctcagcaatc cttacatatt gctactgttg tccttcctaa aaaagagatt gatcccaatg
    19981 accttcctga agctatccgt ccgatcttgc ataaagcgtc acaagctgaa ttagaaaaag
    20041 acaaaaaaaa caaaaaagat gcttatgatg cattgaaaat cgcaaaagaa aaagtgcagt
    20101 tgcgtgagtt ggaaatgaaa atggtcaaag ccgaatacac atttgatcga aataaattga
    20161 ttttttcttt cacagcggac gggcgaattg actttagaga attggtaaaa gatctggccg
    20221 ctgtatttcg tactcgtatt gagcttcgac aaattggtgt ccgcgatgaa gcaaagataa
    20281 taggcggaat tggtccatgt gggcgtccat tatgctgttc ttctttttta ggcgatttcg
    20341 tgcctgtatc aatcaaaatg gcaaaagatc aaagtttatc tttgaatcca acaaaaattt
    20401 ccggtttatg cggacgcttg atgtgctgtc tgcaatatga gagcgaagaa tacgagacag
    20461 cgaaaaaaga actccctgac tttggaaaag taattgctac acctgatggt aaagggaaag
    20521 tcgtagggtt gaatctgctt agccgtatcg ttagtattcg gctgactgga cgggaaaatc
    20581 ctgtcgaata tgaatgggat gaattgaaag aatttatcca agtaggtgag atgaatggat
    20641 aagagatcat tatacgacgg attgaatcaa ttagaatcag agttgagaaa tacattaaca
    20701 gaacttgttg aaatgaagca tgctttgcat gagatcgtag aaaaaaatac aactttggag
    20761 atggaaaatc aacgtctgcg ggaacacctg cgggaattga accaagttgc acaagctcca
    20821 gctgaaaatg tgaagcagga actttctaag tctcgtatga atcttgaaaa aatttatgaa
    20881 gaaggattcc atgtctgcta cgatttatat ggatcaagaa gggacaatga cgagccttgt
    20941 gcgttttgtc tagaagtgat ctatcgcgaa agcggtcgat gaccaataac aagaggagac
    21001 tggagcggag aaatactgct ccagtctttt tattaaggag attgtgacag atgtataatc
    21061 aaaaaagttt caagggaaat ccggtatatg gggatttata tcttgtaccg acgccaattg
    21121 gaaatttaga agatatgagc tacagaagtg ttcgtatgct gcaagaatcc gatcttatcg
    21181 caagtgagga tacgcgtaat acacaaaagt tattgaatca ttttgaaatc catacacccc
    21241 aaaaaagttt acatgaacat aattataaag aacgtgtccc tcaattgatt gctttgttaa
    21301 aagaaggaaa aacgattgct caagtcagtg acgcaggaat gccttccatc agcgatcctg
    21361 gacatgagct agttttagcc tgcataaaag aagggatttc tgtgatctct atcccaggac
    21421 ctacagctgg gatgacggct ttgattgctt cagggctagt gcctcaacca tttttatttt
    21481 atggcttcct tccacgtaag aaaaaagaac agaaagaaac attgaaaaag ctaaaagaac
    21541 agacagttac gctgattttt tatgagtcac cttatcggat cactgctaca ttgacgaatt
    21601 tcttagaaat ttttggaaat cgccaagtcg ttttgtgtcg ggaattgaca aaggtccatg
    21661 aagaatacct tagaggaagt acggaagagc tactcgctta tatcgaagaa catccagtaa
    21721 agggtgaatg ttgtctgctt gtagaaggga atttgttcga aatgccagtg acggaagttg
    21781 atgaagagtt gggaacgtta aaggaacaag tagaagcaaa gatcgcttca ggcgaaaaac
    21841 caaatgctgc tatcaaagca gtagcgctga aaaatgggct gaaaaaacaa gaagtctacc
    21901 gtcagtacca tgaattagac tgagatagta taagttgtct ataatgaaag actaatgagg
    21961 aaatcagcta ctaacttttc aaatctcttg aacgcgaacg aatgttcttg tataatgaat
    22021 aagaggtgag gatgatggag ctgcaatttc ctttacataa tgatacatca agaaaaatca
    22081 ttcatattga tatggatgct tttttcgctt cggttgaaga acgagatcat cctgagtttc
    22141 gcggacatcc attagtaatt gccagacacc ccagtgacac tggtgggcgt ggagtagtga
    22201 ctacagcaaa ttatgaagca agaaaattcg gaattcattc agcaatgagt gcacaaaaag
    22261 cgtatgagct ttgtccgcaa gccatcttta agcctggcga ccatcataaa tacagcgata
    22321 tttccaaaca agtacgggaa gtattctatc gttatacaga tattgtggaa ccagttagta
    22381 tcgatgaagc gtatcttgat gtgaccgaaa ataaaatcaa ttgcaagtca gccatcaaaa
    22441 tcgcccgaat gatccaagca gatatttggg agagtgtcca gctaacatgt tctgcgggag
    22501 taagttacaa taagtttttg gccaaattag catcagacta tcaaaaacct agaggaatca
    22561 cggtagttgc ccctcaagac gctgtgccat ttttaaaagc tttgccaatt gaaaaatttc
    22621 atggggtagg gaagaaaact gtacctagaa tgcatgagct agggatttat acaggaaaag
    22681 acctatatga atgcacagaa atgatgttga ttcgaaattt tggaaaaatg ggctattctc
    22741 tttatcgtaa agtcagagga atccatgatt ctccagtaaa cgttacaaga gagcgaaaat
    22801 ctgtgggaaa agagcatact tatgggcagc cgttgcaaac ggaagaagca gtgctgactc
    22861 agttgcgcca actagctgaa aaagtagaag aagcactgag aagagtccaa aaacatggga
    22921 aaacagtagt tttgaaggtc aggtacacgg actattctac tgtgaccaaa agagtgacat
    22981 tgccagaata tatctataaa aaagaagcac tcttttatca agcaagcctt atatgggagg
    23041 aaatcttggg agtagaaaaa ggaatccgat tgttagggat tactttgacg aatctggacc
    23101 cgatgactta tgaaaacatc gtccttccat tatgggaaaa tcaagaaata taaaaacagc
    23161 tttgagagtt gaaaccggaa atccgttttc tctcccaaag ctgtttttat atcaagtctt
    23221 gctttttgat ttgttcatag gtttctgttg catccgtaca tttttcccaa atcacaactt
    23281 gattggattc taaagatttt tggacgtcaa tgatccgctg gttgctactt ccgcgaaatt
    23341 gtagttttag gtcacgtttg cttaattcaa agcgtccgtc aacaaggata tcgatttgcg
    23401 aaaggagttc taatttatct tctgaatctt gtagcaattc atcaaaggta tagcctgtcc
    23461 atgaccagat atcttttgtc gtaccgaact cttttcggat acgtcgtacg acttgcagac
    23521 aaacatctgt attcaaaaaa ggttcgccac caagtaaagt taatccttgc acgtaatcgt
    23581 tgcgcaagtc agcgatgatt tgttcttcta attcaggtgt gaatggtttg ccatatcgaa
    23641 aattttgtgc agctttgttg aagcagcctt cgcaggcaaa taagcagcca ctaacgtaaa
    23701 gactgttgcg gacaccttca ccatcgacaa aattaaacgg tttgtagtca gcaatatagt
    23761 tttggctgta gtcttttgct aaccattctt ttggttttgg atttctcata aaaaaacttc
    23821 tttcatttaa ggttcaaaga tatagctatt gaagataaaa tctactcgac cacataaata
    23881 agaacaaact gacctaaatc cattgttcaa gaaagcttgt tcttattttg atagagggcg
    23941 ttcaaagata caaagccatc gaaagagggt ggtgaaaaaa gacttgtcat agcctcttat
    24001 tctgaataaa cggtgttcaa aagcttgcat ctgcagtaat aagcccaact aaacgaaaaa
    24061 tatgaggaac tatttttgtt tagctattct tattacttga tgcaggacag ctttttcaca
    24121 acctcatttc atgtgtttca cacgtgagga aatttcttta tggcggccat gtaccatcgg
    24181 acgagcttgc ggatttccta aatagccaca agtacgtttg actacatcac atgtttttgg
    24241 atcatgattt ccacattctg gacattcgaa ccctcgttct gtcggtttga aatcaccttc
    24301 gaaaccacat tgatagcaat gatcgattgg cgtattcgta ccaagatacc cgattttgtc
    24361 atatgcaaaa tcccatacag cttctaaagc tttcggattt tgttggagca tagggtattc
    24421 acagtagtgg atgaatccac ctgaacaata ttttggataa tccttctcaa attccaattt
    24481 ttcaaatggc gttgggtttt tacgcacatc atagtggaaa ctgtttgtat aatattcttt
    24541 atctgtgata ttttcgatga tgccgaattt ttctgtatcc aaacggcaga aacggtcagt
    24601 tagactttca cttggagttg aatagacact gaaatgataa ccatattcat ccccccatgc
    24661 atctgcatta gcttttaatt ctttgacgat atcaagtgtg aaagcttttg cttctgggtt
    24721 tgtttcccat tcgccaccga agaaagctga agcaacttca tacaatccaa tgtagcctaa
    24781 cgatacagta gctcgacggt ttttgaataa ttcattgaca gaatcggtac gtgaaagacg
    24841 tttcccgaaa gcaccataca tatataaaat tggcgcattt gctggaatgg cttctttaca
    24901 acgttctaca cgatagagaa gcgcatcttt catgatttct aagcggtcag acagtaactg
    24961 ccagaattta ttctgatctc cttttgattc gatcgcgatt cttggcaaat tcaatgtaac
    25021 aacacctaaa ttcattcgtc cgacatttac ttcttgacca ttttcgtctt tccacccttg
    25081 caagaatgaa cgacagccca taggtacttt gaaacttcca gttaattcaa cgattttatc
    25141 gtaattcaga atatcaggat acattctttt cgttgcacat tccaatgcta gttgtttgat
    25201 gtcgtaatta ggatcttcag ggtttaagtt cacacctttt ttcaaagtga agatcagttt
    25261 agggaagata gctgtccgct tttcactccc taatccattg attcgaatct gtaaaattgc
    25321 tttttgaatt tctctttcaa accagttttc tcctagtcca aatcctaatg aagtgaaagg
    25381 agtttgtcca tttgaagtaa ataaggtatt gatttcatat tccaaacttt gcatagcatc
    25441 gaagatatct tttttggttt ttgcttttgc atattctttt tggcgttctg gcgaatcgat
    25501 ccattcttcc gcgtctttta gatgtttttt atagttcaat tctgcaaaag gagcaagcaa
    25561 ttcatctgtt cggtcggcag aacagccacc atattgactt gacgctacat tggcaataat
    25621 ctgtgagatt tgtgctgtgg cggtctggat agattttggc gattcgactt cggcattgcc
    25681 gattttgaac ccattgttca gcatgccttt gaaatcaatc aagcagcaat tcgtcatcgg
    25741 tgtataagga tgataatcca aatcatggta atggatatcg cctttttgat gcgcatttgc
    25801 tatatgtggt ggcaacattt tcagtccgat tgattttcca acgatcccgg ctgtcagatc
    25861 tcgctgtgta ttgaaaacat tactgtcttt atttgcgttt tcgttcacga cagtttggtc
    25921 cttgttgatt agcttttcaa tcgaaaagtt gatatctgtt gctttactgc gagcaaaatc
    25981 tcgtttggta cgataattga tgtactcttc tgctaattca tattcgtttt tttctagtaa
    26041 tatatgttcc acgatgtttt gaatctcata gattttgaca ttttcagcaa accggctttt
    26101 gatttcttgg acaaccaagt ctgcaatcat ttgaattttt tcataagtaa gtggtgataa
    26161 tgagccgtga attttctttt ctgctttgat cagtgcttca ttgatctttt gttcatcgaa
    26221 atcaacatgg cggccatcgc gtttgatgac tttcatctgg gatgtctctg gctgctgagt
    26281 aacgacagtt ttttcttcat aatttacttt cattatcttc aactcctgct aaattgttct
    26341 tgtctatcat aaaacaaaat cggggaagga gcaactaaat atcggctata tatagtattc
    26401 aatgtgctgt ataattggat ttctatacta tatgtagtgt atattttatt gtgaaaaaag
    26461 gtgaaaactt gtagctggtg gatttgtttt ttgcatattt aattttgctg aataaaataa
    26521 ttgaataaaa ttttttattc agtccaagta aaagtgacaa aaacataaga gcaaattatt
    26581 cgtagatatg ctactatgca aataaataga aatgaaaaat gaaagaggag gagaagaatt
    26641 ggaaaagatg atcgaagtga aacatctaag taaaagctac ggcagcaaac tttctcccgt
    26701 caaagcctta gataatatct cattcacagt agaagaaggc gagtttgttg ggattatggg
    26761 cccaagtggt gcaggaaaga caacgctgat gaatatcttg tccacgatca atttgcctac
    26821 gatgggcagt gtctcgatcc aaggaaaaga aatcacaaaa atgaaaaaca gtgagctcag
    26881 tgactttcgt cgaaaaaaac taggattcat ttttcaagag ttcaatttga tcgataccct
    26941 aaatgcaaaa gacaatatcc tattgcccct agcagttgaa cggatgacta aagaggaaat
    27001 ggaaaagaga gtaagacatg tagctcaatt gttgaatata gaagaactgc tcaaacggta
    27061 tcccgatgaa ctttctgtcg gacagagaca acgaattgca gctgctagag cacttgttgt
    27121 tcagccacaa gtgatttttg cagatgaacc aactggctct ttggactcga agtcggctac
    27181 agaattgttg aactacttga agaccatgaa tcaaaaagaa aaagcaacga tactgttagt
    27241 cacccatgat ccatatacag caagctactg cgatagaatt ttatttatta aagatggtgt
    27301 gattttttct gaagttgtca gaagagggac aagaagagag ttctttgaga aagtaataga
    27361 catgcaggcg acgattggcg gaggaggaaa aatgaatgct gtataaacta gccgttaaaa
    27421 gtttttttcg gcagtcacga ggctatcttg tttatttttt cagtttgact ttatccgtaa
    27481 tgatctatta ttccttcagc gcaatgacct acgatcaatc tttagtcaga agagcaagtc
    27541 aagatacttc gatagatggt ggattgagtt ttggtggatt tatcattact gttgttttgc
    27601 tttttttcgt attcagtgcc aaccgatttt tcttgaatag aaggcaaaag gaaataggca
    27661 tctatcagct ttttggtatg aataaactgc agatatcagg tatatatgtg ttagaaatca
    27721 tgataattgg attgttcgcc tgtatttctg ggatattact cggtattatt ttttccaaac
    27781 tcttttcaat gattttggtt cgaatgatgg atatggatct tactagtcca ttttttatct
    27841 ccattccatc agtagtagat actttatttg cttttttcat catcctttta gcagtatctg
    27901 tatattcaat atggaaaata tggcgctatc caatgattcg aagttttgga gaaaaagaac
    27961 aagcagatag ttcaacaatg cgcttcagga caaggcaccg attactcgca gtcatcgggt
    28021 tactactttt aacgagcgga tattttggag ctagtcattt tagggaaatc acttcatgga
    28081 tgatcagtta tcattatctt gatacattag ttttcttttt tcctttttta atttttttcg
    28141 tttgtgtggt cggtacttat ttgtttttct gttatacatt tcggttattt ttgatcatat
    28201 tgagtaaatg gaagatttat tactatggta tcaatttttt gataatgggt aacacgcaag
    28261 ttcatttgct caaaagttgg cgtacaaatt ctctgattac agtcattctc ggaatttctt
    28321 taatgacaat tggcggcacg gctagtatct ctactatttt atcctaccag gagaaagttt
    28381 cctccccgat ggactatcag tttgatgttg agacagagaa aaaaatcaag ccgattctag
    28441 cagaggaagg gcagaagata actaaagaaa tcaccttgca ttataaagtc attgggagta
    28501 tctatagtca aaatctcgaa gaacctatgt ttcaaactgc taatctgatc acggaaacac
    28561 aatatcaagc cttcagaaaa gttaatccag atttgccgaa catttctttg aaatcacctg
    28621 gtcatactgt tttgcttgat caaatgcaaa ctctttttcg gaatatttca ttatatggat
    28681 ctagtattgg actgccagat aatcaagaat tgaagattca acaaatgctt ccaagtgtat
    28741 taggtgacga aacaatggta tatctaagtc ctgtactgat tgtagatgat accgtttttg
    28801 aaaaagcaaa aggtgtggat tatcaagtag tcaatgtaga tgtatcaggt ggaaataatg
    28861 aaaaaataga tgaacgactg agcaatgacc tgtcgataaa gtggctcaat gctgtttatt
    28921 attcttatga tatcgtaaat ggaagtctac aaggagaaat tagcacaaag aaacttccgg
    28981 atgaccaaat gaaggaagca caatttagcc aagaaagctc tcgtttgaat ttctcttcca
    29041 gctattcgaa attgcgtgca gttaatcggc gaatcggtat caatattttt gtcacactat
    29101 ttgtcgggat gatcgtgatt attgctactg gtagtatttt gacagtccgg caattagcag
    29161 aagcagagga agaaaaagaa aattatcacc tattgactaa gctggggatt ccacaaagaa
    29221 ggatcagacg gatgatcttc gagcaaaacg cacttacctt ctttccaccc atgatattag
    29281 ggatcactca cgcagtattt gcaatcaatg tcttcactca gtatataaaa actgccgatt
    29341 actggttggc ttatcttgtg agtggcttat tgatccttat ctatcttttg ctttatgtca
    29401 ttacgagtca gttatattgt cggattatcg aggaatgata tacggaataa gacaagataa
    29461 taaatagaac taaattttga ataccattta tttggaataa gaggttgtga cacgattttt
    29521 gtcacaacct ctttattttt tatatttttt aaaagtacga gaaattataa agaaaaaagc
    29581 agtgatttga tgtgctattt ttataaaagc tatgtgacaa aactgttagt aaaaaaacgg
    29641 tttctgtaag ctagacacat gtttcttttt gtcaaaatta gttacacttt gtgtgaggtt
    29701 atatgaggag ggaatccaat gaacaaatgg cgttctaatc caaaattgaa ttattgggga
    29761 aaattcatca ttcgaacaat tttttatagt gcagtactgc tgttcctgat ttatctgtat
    29821 cactataaaa atattcaagg aggaactttt atctataacg aattttagga ggaagagtat
    29881 ggaaatcaaa acgattattg aagcaatcga tgattgggga ataaatgaac ctgatcgagt
    29941 tgcttatcag gcggatgaga cgcatacttt tggtgaattg aaacaagcat cagacgcact
    30001 tgcttattat ttagaaaaaa aggtagaagg cgatggacca attgtcgttt ttggcaattt
    30061 ggagtttgaa atgattgtct catttctagg ggttgtcaaa tctggccatg cctatttgcc
    30121 gattgaagag catacaccca aggagcgtat cctatcgatc tttcgggtgg ctaaaccatc
    30181 tatggtcatc agtatcgggg actggataga agatattcct tcggttcctg tcataacaaa
    30241 aaaagaattt caagaaatca agaagatacc agtaggattt tatcccagaa atagcgtaaa
    30301 aggtacagaa aatttttata ttatttttac ttctggtact actggagaac caaaaggcgt
    30361 acaaatcagt catgataatc tagtcagctt tgttcaatgg acgatggaag actttgggat
    30421 caagcctggt atgcatttct tagcccaagc gccattttct tttgatttat cggtattttc
    30481 tatttatcct gcattagttt ctggcggaat gctgaagccg ttaaataaag cagttgttca
    30541 agactttcgt caattattcg cgacattgcc agccttgaag ttgaatgtgt gggtttctac
    30601 tccatcattt atggatattt gcttgatgga acctacattc aatggagaaa atgtgccgga
    30661 attggacatg tttttatttt gcggagaaga attgacgaaa aaaactgcag aaagtctact
    30721 tgatcgattc ccaaatgcac ggatatataa tacttacggt ccaacagaag caactgtagc
    30781 tatttcaagt attcagattg accagcatgt actagatagt tatgaccgac taccgattgg
    30841 ctacgtaaaa aaagacacga aagtttctat tgtccaagaa ggtaaacctg tttcaaaagg
    30901 agaaacgggt gaaatcatta ttgcaggacc aagcgtatca aaaggttacc tgaacaatcc
    30961 cgaaaagaca gctgctgcgt tcttttgtca aaatgactta gcaagttacc atacagggga
    31021 tgcaggtaga ctagatgaat atgggctttt attttatgaa ggacgtatgg atcagcaggt
    31081 caaattacat ggataccgga ttgaactcgg ggatatcgaa cattatctgc tccaagacaa
    31141 ccgaatcaaa caagctgtcg tcgtaccaaa ataccaagga acaaaagtac agcagctggt
    31201 tgcttttgtc gtattagaaa acaaaacgaa acagccggat tttcagttga ctaaatctat
    31261 caaagaacaa ttactagaag ttgtaatgga ttatatgatt cctcagaagt tccattatgt
    31321 cgatctgctg cctcagacag ttaatggaaa aatcgatcga aaaaaattaa cagctgaggt
    31381 gaatcctgaa tgatgttacc tcatatgatc ccttatgcag agccgtttta tttttttctt
    31441 ttagccattg cgcttgtacc gatcgttcta tctttattga tatgggaaaa aagattacca
    31501 gtttatcaat cagcagtgac acttttcttt ttatttgtca gctttggagg taattattgg
    31561 caacagggag cggcgctgat tgggtatgtt ttgtggcaga cgatacttgt atggggctat
    31621 tttgtttatc ggcaaaggaa aaatcagaca ggatggtttt ttgccgccgt tattttagcg
    31681 ataattccac tgtttttaac gaaagtgact cctttttttg aacatggaaa agcatcgtta
    31741 cttggttttt taggaatctc ttatttaacc tttaaatctg tacaaatgat catggaaata
    31801 agagatggca tgatcaaaac atataagcca tttcgttata tccagtttct gctgttcttt
    31861 ccaacaatct cttctggacc aatcgatcgc tataggagat ttgaaaaaga tcagctccat
    31921 ccgcctgttc gtgataaata tgttgatctt ttgggaaaag gcgtccaaaa tatttttgtc
    31981 ggttttttat ataaattcat tatcggttat tattttggtc aagtactgct tcctgtagtg
    32041 ggacgcattg ccttacagca tggtggtatt tcatgggcat tagtaggtta tatgtacgtg
    32101 tacagtatgt atttgttttt tgattttgct ggctatagct tatttgcagt cggtacaagc
    32161 tacatgatgg gatatcagac accaatcaat ttcaataaac cttttttatc atggaatatc
    32221 aaagaattct ggaatcgttg gcatatgacg ctgtcctttt ggttccgtga ttatgtatat
    32281 atgcgattga tgttcttcat gatgaagaaa aaatttttta aaagcaagat cgtaatgtca
    32341 aatatcggtt attttgcttt gtttcttcta atgggtatat ggcatggatt gacgtggtac
    32401 tacatcgtat atggactgta tcatgcttgt ctgatctgtc tgacagatgc atggatccga
    32461 tttaagaaga agcataaaga gcagttgcct tctaacaaat ttacgcatgg cttagccgta
    32521 tttttgacat ttcaagcagt atgttttagc ttcctgattt tttcaggatt tttagataaa
    32581 ctttttttta gctaacgaat agtgacatat taatttatat aacaaaggag aaaaggaaaa
    32641 tggaagaaca aattttatca atcttagaag aaataacagg aacagatgaa gtaagaaaaa
    32701 atcgtgatat tgatctattt gatgaagggt tgcttgattc tttagcatct gttcaattat
    32761 tagtcgagct agacggagag ctggatatcc aagtccctgt atctgaattc gagcgagagg
    32821 attggtgcac accgaataaa atcattcaac aagcaactgc attgaagggt tgagtatgaa
    32881 aaagaaactt tttgctattt tcggcccggt tgttttagct gttggcttac tgtttgtgtt
    32941 ttttacctcc tcctatcaag tagatatctc taatccagaa acgattagac gtgcctcttc
    33001 ttccatgtca gtgaacgtgt tgaaaggaga tgcgataaaa aattatgcat tatctgaaaa
    33061 acaatatatt cctttttttg gctcaagtga attaagcaga atcagtccat ttcatccgtc
    33121 tgttctggca gaaaaatacc agcgaaacta tcgtccattt ttgctgggag cacccgggac
    33181 acaatcgtta agccaatata tgatgatgcg ttctgcagga gatgctatga aaaacaagaa
    33241 agtcgtcttt attatttctc ctcagtggtt cgttaaaaat ggtgtaaaaa ctgattactt
    33301 taatacgtat tattctgaac ttcaaacata cgactggttg ttttcaatga agaaagtaac
    33361 gcctgctgat cgctatttag ctagaagatt attaactttc tcaaaagtaa aagagaatga
    33421 tacgttgacg gcgattctgc aaacgatcaa aaaagggaag ctacctttac ctgaatcttt
    33481 gaatcagctg cgcagccaat ggaacatgct gaagagagag gatgaagtat tcagcaatat
    33541 tgggttgaaa gatcgacagc aaaaaatcga tcatgagtca aaacgattac caaaacaata
    33601 ccaagaaaca gaattatcca ttttagcaaa tcaaattgga gaacgagaaa cgacgaataa
    33661 tccattcggg ttgaaaaacg atttttatac gcatcgtatc cgagcccacg agccagagct
    33721 caaacagtca caaaaaaatt gggattatcg tttttcgcca gaattttctg attttcagct
    33781 ggtcttggat caattagcaa aaaaccataa cgaagtattg ttcatcatcc caccggttaa
    33841 cgagaaatgg agcgattata ccggactatc tcaagaaatg ttacaaggat ttgccaaaaa
    33901 gatcaaattt caattaaata gccaaggatt caatcgaata gctgattttg tcaatcaagc
    33961 gggtaccaac tattttatgg aagacacgat ccatctaggc tggaaaggct ggttggctgc
    34021 agatcaacag atcagaccgt ttttagaaga aaatcatatt actgctagta agtatcatct
    34081 tgacgatgct ttttttagca aatcttggca acatcaaata ccagataaac tacaattaaa
    34141 ataaagaaaa caggcgagtg ttgctcgctt gttttcttta tttttgaata ttctgaataa
    34201 aagataaaaa taccaagttt gcctgaacaa aaatcataag caatggtata atcacaatat
    34261 gaatatagaa atgggggaaa acatttggat acaaaagaag aacagctact tattgattta
    34321 acttctgcaa aaggagtacc tggaaatgaa gaagaggtaa gagaagtatt cagagaatat
    34381 gccaaaccat tcgctgatga tattttttat gacggtttag gcagtgtgat tgccaaacat
    34441 ggtgctggcg gaggccccaa ggtctttatt tctggacata tggacgaagt tggttttatg
    34501 gtaacaaaaa taaccgaaaa aggattcttg gaattccaaa ctcttggagg ttggtggggg
    34561 caagtcatgc ttgctcagca agttgaaatc aaaacacgtg aagggaaaat cgtccacggt
    34621 gtgattggtt ccaaaccacc acatgtttta acacctcaag taagaaataa accttatgaa
    34681 ataaaggata tgttcattga tattggcgca tcaagccaag aagaagccaa agagtggggc
    34741 atacgtcctg gagatatggt cactccatat attgaataca aacgaatgaa tggcagtaaa
    34801 tatttactgg caaaagcatg ggataatcgt atcgggacag ctgtttctct gcgagtatta
    34861 gagaatctat ctaaagaagc acacccaaat gttttattcg caggaagcga tgtgcaagaa
    34921 gaagtaggct tgcgaggtgc tcgaacaagt acacaccttg taaatcccga tattgctttt
    34981 gctttagata ctggtacagc aggagacact ccaggaatga caccaaaaga agcagattcg
    35041 attttaggaa aaggcccaca gattttgatt tttgatgcat ctatgatccc gcataaaaaa
    35101 ttattgaatt ttgttattga tgtagcagag gaaatggata ttccattcca atatacagtg
    35161 atttctggcg gaggaactga tgcaggacaa atgcatttga caagagatgg tgttccgtct
    35221 gtagccatta ccgttcctgt tcgttatctg cattcccata catcagtgat tcatgaagat
    35281 gattatttga atacagtgaa actagtcaca gaattagtga aacggcttga tagtgaaaca
    35341 gttgaaaaaa ttcggagtta tgaataatga ataatagaat acctatttct gaatatcgga
    35401 aaaaagcacg agtttctttg gaaaatcaat ggggaatcaa tacttggctt gttttcttat
    35461 ctataatatt tacgagcgtc atccagaact tttttgacgg tttcagtcct tttccggaag
    35521 aaagcatgca gcagcactta ttgagttttc tagtcgataa tttactttta ttcgcattca
    35581 actatgcatt gttttacatt gcgctagtag ttgtacgggg tggcagacca aaaagtaacc
    35641 ttttattttc agttttccaa agaaagtatt ttggttcgat acttggcatc aatttgatca
    35701 attcagtcat caactggcta ctcaacagtt taatctttct gcccatgttt ttaatcagcg
    35761 gagtgagtac ttatatgcag ctgcttttca gttcaaatgc ggtacctacg ggtgtcatgc
    35821 cagaagattt tcttgatatc agctttgttt tatcgatgat agggatgatt tttgtttcag
    35881 cactcatcat gagtattgtt ggcggtttat tccaatttgc tgcatggaca aagttcgact
    35941 atcctgattt gtcggttatt caatgtttga aatatggctg gtttttattg aaagatagaa
    36001 ttggtacgta tattttgttg cagctgtctt ttatcggctg gtatttgcta ggagcaatcg
    36061 ctttgttcat cggtcttttg tgggtcattg tttatgtgaa tgtgtcagtt gcagaatttt
    36121 atgaacaagc acggattgaa aaaggaggtc cagacgccta ttttacttag catgtaagca
    36181 gtttattcat caaaaaaata acttttgtgg taggataaat ctttgagggg gaatgtacga
    36241 tgaaattaac tgttttagga tgcttaggag cgtatcctta taaaaatcaa gggacaacag
    36301 gttatttgct tcaatctgac gggttcaatt tgttgatcga tgcaggtagt gctacgctga
    36361 tcaaattaca agaacacctt gatccacttg atctagatgc tgtgatcctt agccattatc
    36421 atcatgatca tattgctgac cttggtgtat tacagtatta ctggcaactt catcctaaga
    36481 gaaatccgga gacattgctt tcaatttatg gacacacaaa agattccatt cactttgaag
    36541 aattgacgtt ggaaaatgtc tcaaaaggac atccttattt tgaagctgaa gaattaaaaa
    36601 ttggtccttt tctgattact tttatggaaa caatccatcc agtagtatgt tacgccatgc
    36661 gtatcgtaga agaaacaacg gggaaagtat tcgtatttac aggtgattca ggttatttgg
    36721 aaagtttcgc tgattttgcg aaagatgctg atttgtttct agcagatacc tatctttttg
    36781 aaggaaatga acatcatcat gcccatttca cttcaaaaga agctggagaa cttgcgaaat
    36841 cagctaacgt aaagaaactt gtacttacac acctacctca gtttggtgat ttagaacaat
    36901 tgaaaaatga agcgcaaaag gcagcaggca aagggattga tatagaactt gctgcagttg
    36961 acaaagtgtt taacatttag gaggaagaac atgatttttg taccaaatga aaaccacgac
    37021 ccaagaatca atttagcaat tgaacaatat ttgctgcaag aaatgccttt agatgaacct
    37081 attttgctct tttatatcaa tgaaccttca atcatcatcg gcagaaacca aaatacgctt
    37141 gaagaaatca atcgagatta tgtggaagaa aatggcattc atgttgtccg tcgcttaagt
    37201 ggaggcggag ctgtctacca tgattttgga aacctgaact ttagttttat catgcctgat
    37261 gatggggatt cattccgtga ctttgcgaaa gtaacacagc caatcatcca agcacttcat
    37321 gaattgggtg ttgctggggc ggaattgaaa ggccgaaatg atctagtcat tgatggcatg
    37381 aaattttctg gcaacgcgat gtatgctacc aacggtcgga tgtttgctca tggaacaatt
    37441 atgttcgact ctgatatcaa tgaagtagtc aatgcattaa aagtaaaaaa agacaaaatc
    37501 gaatcaaaag gaatcaaatc gatccgttct cgtgtaacga atatcaaacc atttcttcca
    37561 gaagaaaagc aaaaaatgac aacagaagaa tttcgtcaag aaatcttgtt gaaaattttc
    37621 ggcgtagagt cagtagaaga agtaaaaacg tacgaattga ctgatgaaga ttggaaaaaa
    37681 atcaatcgaa tctctgagga atattatcga aattgggatt ggaattacgg gaagtctcca
    37741 gatttcaatt tctctcgcca aaaacgtttt tctatcggtt cgattgaagt ccatttgaat
    37801 gtctcagaag gaatgatcaa agaagcaaaa atctttggcg atttcttcgg actaggagat
    37861 atctctgacg tggaaaaaaa attggttgga cagaaatatg ataaagcatc tctatcaaaa
    37921 atagtcagcc aaattgatgt gaagaaatat tttggtgcga tagaaccaga agaattgctt
    37981 accttacttt attgaaaata aaaaatagcg gaatccacat atatggattc cgctattttt
    38041 tatacaggat tcgggtaatt tcgagagagt tgaacagtga tccgatcaat gaaagtatct
    38101 ttttcctgcg ttagtgttcc tgtgtaacga tccgctacgt ctttcatcat cattaagtca
    38161 aaacgttcct gatcatctaa ttcttctgtg atgcctcgac taaataggtt tgtatcgtaa
    38221 tgtgcactat gttcgcaaca aatgacgaaa tgttcctctt gttcgtgtat cgacaaataa
    38281 atttttcgat acagtggatt ttcgatggca tgagtttctc taaaggaatg ctctaaagcg
    38341 tggatcaaaa gttgagccag atcgtcatcg ttgataggaa gttcttcggg aagatctgct
    38401 tggaaggtta tctgaatatt ccgtttggca gctaacttct ggtaacgcgc taagataaga
    38461 tttgtcacct ggtggttgca aaaagaacac agcatttcga aattttttgc ttcttctaat
    38521 atcttttcca catagttttt agcttttgtt tggtccgtat gcattaaatc ttgcagattc
    38581 tccatctctt ggacaaattc caggcgaagt gtatttactt cttggatatg ctgtcgaagc
    38641 tgctcataat gttgctccaa caaatcattt ttgattttta agaaatctac ttgtctccga
    38701 tctttgtcgg tatttaagat gtgatcgatt agattgtagc tgataataac gattagaatg
    38761 ataatatata aaatcgtaga ccaattgatc gttgtttgat agatacctaa tgccgtttgg
    38821 atgtaaatca agcaatggat aatggctacc aagacgatcc aaggtgtaca agctacataa
    38881 aaacggttat tcttatatgc ttcgccaatg catgcaagtg aagtgactaa agtgcttata
    38941 acactagcaa tattagcaat cggcattaat tcaggaagtt ctgcaggagt aaaagttgca
    39001 taaagcagaa cgccgataaa aatcaaaagt tggaacgtac aaaaaattcc gtaccatttt
    39061 ttatagtaaa acattcggct ccaatagtag cagatgatga aagctgacgt taagataata
    39121 aataaatgag ataaaaaaga atgaacgaat ggttcaaaaa gaataccact taaaatatct
    39181 tctgatacag cttcaaatcc taatgccaaa gcaaagcaac ttaagagaat aagtgagtat
    39241 tcaagacgtt tttgtttata catcaagtag atcgtataga aaaaggtccc tatagcgata
    39301 aaaacagcga ttatcatcgt caatacttgc ggcactgatt gtgaataaat aaaactaatc
    39361 aatggtccag ttgttcctag ataaactttc ggtggcagtc cagcatagtt ctgataagga
    39421 gatgagactg taagggtcaa tttttttccg atataatttg ctggtaaatc tatcacagcc
    39481 aatgagagtc cgggattgct ttcttcctga taatctttcc gttggtataa aatcttttcg
    39541 cctagatata cagtaatcca ttgatggttt cctttgatta gtaaatttgc ttcgtccaac
    39601 ggtttttcaa gtgtcgtttc cataatgaat gtttcgtctt tatccacggt aggaatcgta
    39661 cgagtgtact tgctgtcaaa cttaaaatca ggatcatctt ttgtatagaa aacccacgaa
    39721 tcttttattt ctttcatttg attgctgtcc gcaggagaaa aaataaaata aaagaaataa
    39781 gcaattcctg ttaaaaataa ca
//
LOCUS       DAZAID010000016        39635 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000016 DAZAID010000000
VERSION     DAZAID010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.016"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..574)
                     /locus_tag="ACG9BE_000773"
     CDS             complement(29..574)
                     /locus_tag="ACG9BE_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFN3031549.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(586..1278)
                     /locus_tag="ACG9BE_000774"
     CDS             complement(586..1278)
                     /locus_tag="ACG9BE_000774"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFN3031550.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1297..1581)
                     /gene="macP"
                     /locus_tag="ACG9BE_000775"
     CDS             complement(1297..1581)
                     /gene="macP"
                     /locus_tag="ACG9BE_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFN3031551.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1594..2157)
                     /locus_tag="ACG9BE_000776"
     CDS             complement(1594..2157)
                     /locus_tag="ACG9BE_000776"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFN3031552.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2385..3047
                     /locus_tag="ACG9BE_000777"
     CDS             2385..3047
                     /locus_tag="ACG9BE_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFN3031553.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3171..4367
                     /locus_tag="ACG9BE_000778"
     CDS             3171..4367
                     /locus_tag="ACG9BE_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFN3031554.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4468..5844)
                     /locus_tag="ACG9BE_000779"
     CDS             complement(4468..5844)
                     /locus_tag="ACG9BE_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3031555.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5881..6748)
                     /locus_tag="ACG9BE_000780"
                     /pseudo
     CDS             complement(5881..6748)
                     /locus_tag="ACG9BE_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(6837..7862)
                     /locus_tag="ACG9BE_000781"
     CDS             complement(6837..7862)
                     /locus_tag="ACG9BE_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3031556.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7880..8554)
                     /locus_tag="ACG9BE_000782"
     CDS             complement(7880..8554)
                     /locus_tag="ACG9BE_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3031557.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8738..9631)
                     /locus_tag="ACG9BE_000783"
     CDS             complement(8738..9631)
                     /locus_tag="ACG9BE_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN3031558.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9705..11402)
                     /locus_tag="ACG9BE_000784"
     CDS             complement(9705..11402)
                     /locus_tag="ACG9BE_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN3031559.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11395..12708)
                     /locus_tag="ACG9BE_000785"
     CDS             complement(11395..12708)
                     /locus_tag="ACG9BE_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFN3031560.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12708..16649)
                     /locus_tag="ACG9BE_000786"
     CDS             complement(12708..16649)
                     /locus_tag="ACG9BE_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN3031561.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16758..17216)
                     /locus_tag="ACG9BE_000787"
     CDS             complement(16758..17216)
                     /locus_tag="ACG9BE_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN3031562.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17514..18032)
                     /locus_tag="ACG9BE_000788"
     CDS             complement(17514..18032)
                     /locus_tag="ACG9BE_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFN3031563.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18034..18651)
                     /locus_tag="ACG9BE_000789"
     CDS             complement(18034..18651)
                     /locus_tag="ACG9BE_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFN3031564.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18648..19502)
                     /locus_tag="ACG9BE_000790"
     CDS             complement(18648..19502)
                     /locus_tag="ACG9BE_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3031565.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19499..20410)
                     /locus_tag="ACG9BE_000791"
     CDS             complement(19499..20410)
                     /locus_tag="ACG9BE_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3031566.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20451..21785)
                     /locus_tag="ACG9BE_000792"
     CDS             complement(20451..21785)
                     /locus_tag="ACG9BE_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3031567.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21993..22823
                     /locus_tag="ACG9BE_000793"
     CDS             21993..22823
                     /locus_tag="ACG9BE_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFN3031568.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23067..23316)
                     /locus_tag="ACG9BE_000794"
                     /pseudo
     CDS             complement(23067..23316)
                     /locus_tag="ACG9BE_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23507..23701)
                     /locus_tag="ACG9BE_000795"
     CDS             complement(23507..23701)
                     /locus_tag="ACG9BE_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031569.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24206..24391)
                     /locus_tag="ACG9BE_000796"
     CDS             complement(24206..24391)
                     /locus_tag="ACG9BE_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031570.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25241..25582)
                     /locus_tag="ACG9BE_000797"
     CDS             complement(25241..25582)
                     /locus_tag="ACG9BE_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN3031571.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25582..25812)
                     /locus_tag="ACG9BE_000798"
     CDS             complement(25582..25812)
                     /locus_tag="ACG9BE_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN3031572.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26099..26437
                     /locus_tag="ACG9BE_000799"
     CDS             26099..26437
                     /locus_tag="ACG9BE_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031573.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26642..27204)
                     /locus_tag="ACG9BE_000800"
                     /pseudo
     CDS             complement(26642..27204)
                     /locus_tag="ACG9BE_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(27936..28517)
                     /locus_tag="ACG9BE_000801"
     CDS             complement(27936..28517)
                     /locus_tag="ACG9BE_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3031574.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28700..29326)
                     /locus_tag="ACG9BE_000802"
     CDS             complement(28700..29326)
                     /locus_tag="ACG9BE_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFN3031575.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29348..30679)
                     /locus_tag="ACG9BE_000803"
     CDS             complement(29348..30679)
                     /locus_tag="ACG9BE_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFN3031576.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(30834..31235)
                     /locus_tag="ACG9BE_000804"
     CDS             complement(30834..31235)
                     /locus_tag="ACG9BE_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN3031577.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31321..31527)
                     /locus_tag="ACG9BE_000805"
     CDS             complement(31321..31527)
                     /locus_tag="ACG9BE_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031578.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31622..33124)
                     /locus_tag="ACG9BE_000806"
     CDS             complement(31622..33124)
                     /locus_tag="ACG9BE_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFN3031579.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(33142..33597)
                     /gene="argR"
                     /locus_tag="ACG9BE_000807"
     CDS             complement(33142..33597)
                     /gene="argR"
                     /locus_tag="ACG9BE_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN3031580.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33695..34312)
                     /locus_tag="ACG9BE_000808"
     CDS             complement(33695..34312)
                     /locus_tag="ACG9BE_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN3031581.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34533..35906
                     /locus_tag="ACG9BE_000809"
     CDS             34533..35906
                     /locus_tag="ACG9BE_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3031582.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            35981..37093
                     /locus_tag="ACG9BE_000810"
     CDS             35981..37093
                     /locus_tag="ACG9BE_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN3031583.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(37165..38802)
                     /locus_tag="ACG9BE_000811"
     CDS             complement(37165..38802)
                     /locus_tag="ACG9BE_000811"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFN3031584.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(38970..39578)
                     /locus_tag="ACG9BE_000812"
     CDS             complement(38970..39578)
                     /locus_tag="ACG9BE_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN3031585.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT        11375 a         8436 c         6886 g        12938 t
ORIGIN      
        1 ttttatttta tcaaatcact ttgttttttt ataaaatttc tgcgcctagt gtggactcaa
       61 aatgtctcaa tgcccattca tgtccaacgg gatcgaaact tgccacggaa tctttgaaaa
      121 tatggagttt gtatcctaga ttatacgcat cgactgctgt atgcaacaca cagatatctg
      181 tacatacgcc ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg atgtctagat
      241 ctgttccact aaaagcggaa taatgccgtt tgtctatcca ataaacattt tcctcttgct
      301 catagtcttg gtataaactt tttaaagagc cgtacaattc ccgtccaccc gttcctatca
      361 cattatgcgg aggaaataag cggttctccg gatgaaagga atcttttgga tcatgcgcat
      421 caatggcaaa aacgacaaaa tccttttgat caataaactt ctttgtatga gcagtcagct
      481 ttttttcaat tacttgtcct gctgtcccag tagtcagctt tccatcctct gcaacaaaat
      541 catatgtgta gtcaatcgag atcaatgcgt tcatcttagt cctccttata cgagatgttt
      601 gacaaattcg atcaccattt ctgctgatcg tttgcctgcg tcttcgataa attcgtcaaa
      661 gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca cgtacgatca aaaatgggat
      721 attgaactga cgagctgttt gtccaacagc ggctccttcc atttcgcaag ctaatgcttc
      781 agggaaattc gttaagattt ctttgatttt atctggcgag tcgacaaatg tatcacctgt
      841 aacgatcaat ccttcttttg cgttcaaatt cgttgctttt gctgctttaa ccatttcact
      901 gcgcaaatat gtgcttgctt cataatatag aggcattcct ggtaattgtc ctgggttgta
      961 gccaaatcct gttgcatctg catcaaaata agctactttg tctgatataa cgatgtcacc
     1021 tacttgtaag ccttcaccga ttccgccagc tgaacctgta ttgatcacca tgttcacgcc
     1081 gtattgttgg atcaacagac ttgtggtaat agatgctaag acttttccta taccggaacg
     1141 tacgacgatc acttcgtggt tacctaatga acctgaaata aataaagcgc ctgcgcgttc
     1201 ccatgataat ggttcgctta ggttctctct caaaattttt acttcttctt ccattgcgcc
     1261 gataataccg attttcatct ttttaattcc ttctttctat ataaatgcga ttgctaaaaa
     1321 aaccacacat aacattagga taacaataat aagagatttc atcaaaaaag agttccattt
     1381 ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt ggtttattct ttttgctttc
     1441 tttacgataa aagctggcaa tctttttttc ttcttgctga taagctgttt ctgcttttcg
     1501 ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt cgcttcgcgt
     1561 aataagcggt cctttggcca taagccgatc cctctacttt cctttggtca atagttccca
     1621 gtattgaatc gcataaatcg tctttgcatc acaaatcaac tgatctttca tcgcttgttg
     1681 cgcctcttca agagttagat gatacaactc cagtacttcg tcttcatctt gtgctaaagg
     1741 attctcgacc ttctctaccc cttgtgcatg ataaatatga aggacttcat ttgcaaaacc
     1801 tggtgaaaga tacatcgaag taagatggct cagcgacttc gcacgatagc cagtttcttc
     1861 ttctaattct ctagctgctg tcatttcggg attttgtcct tctcctggat cgatttttcc
     1921 agctggaatc tccaaaatca ctttttctaa aggttttcga aattgtttga caagaagcag
     1981 acgatcttgt tcgtcaaaag caatgatccc gacaccgcca ggatgaaata ccagttctct
     2041 cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa taatttttcc
     2101 gctatagatt tctttgcgac tgattgtttt ttcttcaaat tgctcattgt tcagcatatc
     2161 ttctcctgct ttctttgtaa tcataactgc ttttttttca tttttcaatt tttctcaaaa
     2221 actccttatt agtttaccat agataaacct tagattttct ctaaatatag aaaatccttc
     2281 tttagtccga tactctttct agtagtccgt atgctaaaat aagtatgtaa tcgaataacg
     2341 aaaagaattc aatactcaac gataaatgac atggagggtt tattatgata aaacgaaatt
     2401 ggccgtggct tctagcattg gtcttgctat ttctctttgt ccgaaacaat agtttttctt
     2461 ttgttctagg acttttattg ataggtgtag ttttagtttt gtggggactc tttggttcgc
     2521 gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac tgacgaactt gaaactcact
     2581 atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca atgaatcaaa
     2641 caaaaaatga gatcaatcaa ttgcaaatga atatgcagca gacagccaaa cttaaagcaa
     2701 tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa ttggtgaaag
     2761 aaccaaaccg actgcatgaa gcaagtcagt ttctttatac gcatctgccg aatctagttg
     2821 atttgacaaa taagtataat gagatcaatg accatgagat caaaaacaaa cagacctatg
     2881 aaaaactaga agaaagcgcg cagatcatcg accagctgtc agctttaatc gcacaagatt
     2941 atcaaaaatt cgttgctgag gatctagatg atatcgacgt agagatatct gttgcgaaac
     3001 agagcttaaa gcgtgataat aaatatgaac cagaacaaaa ggattaactc ttttgttcat
     3061 aaaagaagac tcggaacatt cagatgatgt ctggctgttt cgagtacttt ttctaaagta
     3121 gagacattca tgtaagaatc gagaacagat tcaacaagga ggaatttttt atggatcata
     3181 aaccgactga aacaaaagat gttacaccag ttaacgatac acttgaagat ttattgaaca
     3241 acccgttttc aactccaaca gatacgctga ccacttcaca acaagcggag attgatcagc
     3301 tccaagagca acagacagct gctcgtttaa ttgacaaact accggcggaa cgccaagaac
     3361 aagctaaaca gctagcagca aaaatcgatg ctaatgatgc acagtctgtc atcagctacg
     3421 gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat catgtgcaag
     3481 cacaagatat tggtcctgta ggagattcct taactgaact gatgtatcgt ctccaagaag
     3541 ccaatcctga cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc ttcgggaagg
     3601 tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa gattggtgca caaattgaca
     3661 agatttcagt gaaattagat aaagaaaaag acggtctgct aaaagataat ctgatgttag
     3721 aacagcttta ccagaaaaac aaagactact ttgatgcact gaatatttat atcgctgctg
     3781 gagaattgaa aatggaagaa cttcaaacaa cgatcattcc agaagcgatg aaaagagcag
     3841 aagaaactgg cgatcagatg gatgttcaga ttgccaatga ttacacgcaa ttcctagatc
     3901 gtctggataa acgtacgcat gatcttcgct tagctagaca gatcacgatc cagcaagcac
     3961 cacaaatccg tttgatccaa aacacaaatc aggccttggc tgaaaagatc caagcatcta
     4021 tcgctacagc cattccgctg tggaaaaacc aagtcgtgat cgcattgacc ttgcttcgtc
     4081 aaaaagatgc agtaactgct caacgccaag tctctgaaac aacaaacgac ttattgaaga
     4141 aaaattcaga aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat gaacgtggaa
     4201 tcgtggatat cgagaccctt caaaccactc aaaatgattt gatcgaaaca atccaagaga
     4261 cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta gagcttggac
     4321 acatggaaga agatctgaaa caaaaactgt tggatttaac ccaatagatc acctttcgct
     4381 tgttactaaa acaaacgccc aacaacagtt tattacaact gttgatgggc gtttttctta
     4441 tctttcttct aacttctcca ttggagtcta tttctcattc atcttgctgt gtccatagcc
     4501 atagccaaaa taaatgatca gtccaataac cattacgata ccgaacacga tccatgtcac
     4561 agtttgcaga cgaagcatca atacaataca agtcaaaatg gagacgattg gcaatagcgg
     4621 aacgaatggc actttgaatt ctccagcttt tggtttgcca aacatttttc tcaacttcag
     4681 taagcctaga ctaacaagca tcaaataggc gagcgccatg atattcgtta gttccgctaa
     4741 tacattcaat gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg cataagtcgc
     4801 ttttttcggt gtacgatgct tttgatcgat ttctgtcata aacttaggaa gtaagccatc
     4861 cttgctgaca gcaaacagta aacgagcaag tgaatacatc atcgagatcg ttaccgtcag
     4921 tagcgttaaa attgccccta cagcgatcac cgcgcccact gttccatgtc ctacatacct
     4981 cattgcaaaa gccactgggt cattgactcc tagttcagta tacggaacga ttcctgtcaa
     5041 gatcagcgtc acgatgatgt aaaggacagt agcaatcagt atcgaaccaa tgattcctct
     5101 tggcacatct ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg catcaaatcc
     5161 caagaaagca aaaaacacaa gagctgcacc atctagcaca ccggaaaaac caaaaggcat
     5221 gaatggctgc cagttatcag gtttaacgaa aaatattcct acaatgataa acaacgcgat
     5281 gataccaaat ttgacatata ccatggcatt attcaaacgt aatgctttct tggcttcaag
     5341 cgacacgatg taagtgacga atatcactac gagcaccgca atcaagtcaa tgtatgtacc
     5401 attttccgga ttgtacccgc cagttaatgc ttgtggaagt tcggccccta aacttttcaa
     5461 aaaaccttgc atatagccag accatccgga agcaacagag gaaaccgcta gtaaaaattc
     5521 cccgatcaac aaccaccctg tcagccaggc agcaaattca ccaaatacaa catacagata
     5581 ggcatatggc ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac ttagtccaga
     5641 caaaatgaca acgattgcag ctaagacaaa tgaaagtgac agtgctggac cagcattttg
     5701 attggccgca acccctgtca ccacaaatat tcccgtaccg atgatcgcac caattccaag
     5761 catgatcaaa tcagacgttt ttagttcttt tttcatctca ctcgtatgat tgagattgat
     5821 ctctttctta cgaaacaaat ccattgaagt tcctccccat tttaggttgg aaaacagcga
     5881 ttaaactgtt ttcatatacc ctaaaaggga taccacctaa aatgactaaa aggcaaccaa
     5941 caataacata aatgagctct tttttcgttt ttctttcgcc aaggatgaaa atcccaccta
     6001 aagtagagat aatgatcccc atttgtgaaa gtgagaagcc aacagctaaa ccaagtgatt
     6061 tgactgttag aagcatgcag atgtttccta agccccataa taatccacag atcatgtttt
     6121 tccacacaaa ctgatcgact ttaactttac ggaaagcaaa gaagcttgcg cctaagatca
     6181 ttccgatact ttgaggtaag ataattgaca tcgcatccaa attcgcccag ttgacgatga
     6241 tggtatatac accataacca atcgtagaga agatcaatgc tctaaaaccc ttaccaaaat
     6301 caagcatttt attatctgta tctatttttc cttcgtcatc ttgtcgggca gtcaaatacg
     6361 caccgcttac aagaagagct aaagcaataa taccgtatac ataatctttc gtttgtgtcc
     6421 attcatggaa gaaaacggct cccgcaatcg tattcaaaat taattgaaaa ccagtagaca
     6481 atggtagccc tacagaaaca cccatgtact tcattccatg gaactgtcca ttttgtccaa
     6541 cactccaagc taagccggat aaaaatccga tcaagaaaat ccaaggggtg attacaggac
     6601 taacaacgaa aaagacaact aaagaaaaca gaaatgcacc gatcgtcatt cctagtgttt
     6661 gttgattggc atcgccacca attttaccac ttactaaacc aatactaccc catgccaaca
     6721 tagggactaa tgctattaaa atttccataa ccaatctcct tttatttcac atgataagaa
     6781 acttatcagc gaaaacgtta acaacacaag cacttttttt cgtttctaac gattttctaa
     6841 cgtttttcat tataaaaatt taagattggg aaaaataaag tcattgttgt cccttttcct
     6901 tcagttgatt cagccgtcaa atggatacct aatttttcgg ccagattttt tgctaaataa
     6961 agtcctaatc ctgttgaatg tgttttgcta agtcgaccat ttctcccagt aaatccctta
     7021 tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc ctgaatcttt aactgaaagg
     7081 ctgactcctt gcgcttcctt ttctatgctc acaatgatgt ttccgtgatc cggcgtatat
     7141 ttgatggcat tactaagcaa ttggttgaag ataaaagcga tccatttagc atcggtcaaa
     7201 acagtatgat cctcaccaac gatctcgtag cgaatatttt tttggataaa ataatttgcc
     7261 tgactgcgta tcacaggctg aatgattcct tttagtccat attcttggat gaggtagtcc
     7321 cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt ctacgtattc atcgattttt
     7381 gataattcat tttctagtaa tgtatacttt tgatcgtcaa tatcgaactc gatcgaacga
     7441 actagcaatt tagaagcgga aagtggcact ttgatttcat ggacccatga gtcaatataa
     7501 tctttttggt cttgttgatt actgattgct gattgcatta cttcttggtg ttcacggatc
     7561 agctgattga tatattcttc taccagtttt tcttcttctg tcctcccacc actcaaataa
     7621 ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc cccaccatct tctcttgctc
     7681 aagtaaccac ccagcagata aaaaatcaga aagacgccct caatcaaagc taaataagca
     7741 attgtgctca aatcaacttt gatgtttggc gccagccaca tcacaaagat cgtcaatgca
     7801 ataaaaaaca gccatcccaa gatcaatgtc cactgatctt tcaaatattt aaaaaaattc
     7861 atcttaaccg ctcatccttt cagggaacga tatatccctg tcctactttt gtttcgatat
     7921 atccttggat acctgcctgt tcgatttttc ggcgtaaccg attgatgttg actgtcaatg
     7981 tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg cagcaatttt tcccgactta
     8041 agatctttcc attgtgcttc atcaaaataa ataatagacg atattcattt ttgctcaaat
     8101 caatgatctc tccgttgatt tccatacttc cgttgtcaac attcaacgta atgccattgt
     8161 ggctcatcat ttcactagat aattctgtgt agttgtaaga acggcgaagt aatgcattga
     8221 tttttgcaat cagtacatcg atttggaaat gttttgtcac aaaatcatct gcccccatgt
     8281 tcatagccat gatcatatcc atattcgtat tcctgctgga gataaaaatg atcggtactt
     8341 tcgatacttc acgtattttt tgacaccaat aatagccatc caaaacggga agattaatat
     8401 ctagtaatac aagctgtggt tcttctcttt caaagtctga gaacacttga ttgaaatcgg
     8461 tcgttccaaa ggtttcaaat tgccatttct caagttcctc catgatcaat tgtcgaatga
     8521 tctcttcgtc ttcgactacc atgatttttg ccataaaaaa tccctccatc tttcttcttg
     8581 ctactacaat atcaaagaag cccttgtgaa acaagttggc ttacctggaa aatgtcattt
     8641 tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc gcgtttgtct cttccaagca
     8701 gcttaaattt attatgttat tcttaataat tggtctatta tctgttattt ttctttcgtt
     8761 gtttttgtct tttaaatttt cgataacacc aataaccgaa aagcgttagc actagcaata
     8821 tacctagaag gagtataagt aatcgataaa gatggtattt ttttgtctgt tctatctttg
     8881 aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac tagtaatcgg tgagtgttca
     8941 cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg cttttctcta attgctaatg
     9001 tatttgtttt tgttggaagg acaacaattt tctcaacgac ttcataagcg agcatccgac
     9061 catacacttc aagatagaat cggtcatctt gctccatttc ttcaagatcc gtaaagagtt
     9121 ttttctcagc aataccgcta tgggcggtaa tcacggaatg tgtactctct cctccagttg
     9181 gaaaagaggt gccttgcagg actgtcgctc ctctatctaa caaaagatca ttcgtttcat
     9241 caaataaagg caagctaaca ttgattttcg gaatatagat ggctccgatc atatgttgtt
     9301 cgtaatattc ttttcctgga ttttggacat ttctaaccgc ttgctcaaaa ggatctttga
     9361 cttgtcccat tcctgggatt actgatgtat gagcaagcgt ctgatttttc ttttcatgca
     9421 ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt tgataattct
     9481 cgatccttct ttgatccaaa taattgctga gagcatctgc taaaaatgga tatgtaaaaa
     9541 ctgcagctcc ggcaagaaac atcactgcca taaataattg taataaaaga ctgatttggg
     9601 tttgaggtct attacctatt ttctttttca ttttcttcac cttcttattt aaaaaaactg
     9661 cagcaacctt tataaaaccg attgctgcag cttatttttg ttgcttatac ttcagacttc
     9721 attttagatt tacggtacca gctgtacgct ccaatcatca aacttattcc gattagtaag
     9781 aatgccaaga gcccgttccc accagtagca ggaagtgacc cgcgtttcat attcggtact
     9841 ctttctggcc ctggattttc tactcctacg atctgaattt cttcattata gcttccataa
     9901 gagatgataa attcagtaaa ggctgcctca cctgttggaa gtacataacc ttctggcgcc
     9961 atggtctctt tcatttgata ggttccataa tctaaccctt gaacctcaaa gtatccattt
    10021 ccatctgcga caaattctgt tgcttgtccg cttgttgccc actcaattct atctgctcct
    10081 gattcataaa cagctaaagg cgactcccct ttataaaata ttgcgtattc agaacggtct
    10141 tcattccaca attcgaatct tgcatcttta agcaattcac tgcttgatgc atcatatttt
    10201 tcaaactttc ttccacctgt tgtgactttc tctggcggct ctactccttc ttcataatca
    10261 aattcatcat ttcgtccgat ttcaataacc gctgtgttgt tatggaaatc atccgcttct
    10321 agttctgtgg aaaccttcaa attatacgtg atcgttaaat ctcctccacg tgcatctgct
    10381 aatctaccta aagtatctac tttggctact ccattttctt ctgtcaaaat cgtcacgaca
    10441 aacccgttgc ttactacttc tactatataa tcttgtcctt ttgttaaaat cgtctcgccg
    10501 gctcgcactt ctatttcctg atcataataa gttagaccaa ctgtaggcgt atctgtgatt
    10561 ttgtaatttt tgataacagc gcttccgttt tcaaggacag aatcgatgaa ataaggaata
    10621 ggtgctgtga tcgtgtaaga agccgttttt cctctttcta gatcaatata agaaatcgtt
    10681 tcttgttctc cagcttcatt aataatcgtt acttgtctaa ggtcgctttc ctctacattc
    10741 atctgtttat ctgcttctct gatttcgttt ttcggataca aatgcacatc tgtattgtat
    10801 gtgtctttcc atgtagaacc atcccacacg ccatccacca tatcaggcat catcactggc
    10861 atcataacaa ccattggaga cgcgattctg gaaatatgag ctggcgaata agtttctaag
    10921 aaaagataga ctttatctct agaagaaggt gcttcttccg tcacttggac ggtagagaat
    10981 accgcttctc cagcttcatt cgttgtttgt ttgtcaatac ttgtcagtcg atattttaaa
    11041 aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg cttcggcaga attaagaccg
    11101 tcttctcgtt ccggtgttaa aggattatct cgaatcaaat cgtaatatac ttccgttacg
    11161 tcaaacaaat caaaatcgac tcctggaata ttttcttcac caaaatccgt agaaaccaat
    11221 ccgctattcg gtcgttcttc cggaacagat gagaaacctt ttttgtgtaa tgtaatcgtc
    11281 acttcacttg gtttttgtgc gacttcttcg gctttgacta gttttccgtt cccgaaaatc
    11341 cctattcccg cagtaacgag taacagtaca ctcattcccg tgacaatcat ttttttattc
    11401 atttttttct ccccgttctt tttttattaa taaaacagca cctaacaata aaagaattcc
    11461 tagtaaagaa atcaacgttc ttgcttcatt cgtttgcggg aatacggacc attttcctga
    11521 ttgacctgga ttgcttggac cactcggctc tttcggtttg tccgttgttt cttttacggt
    11581 ctcgttataa acacgtggtg ttgcggattc ataggcagag actggaactt tcccggattc
    11641 taattcagcc atctcctgtc cgttaactac tacatattgt ggttggcctt ttgcatccac
    11701 ccaaaattca gggatgatga cctcgattct cttacttgct tcatccatct cgtaaccttc
    11761 cacacctttt aattcttcaa aataataggt gccagaaggc aagaaacgtt gtcctgtcgt
    11821 aacaaggccc tctttatccg aaatgaattt gcttacattt ttgtcattca aaggatcatt
    11881 gtctgctggt ttgatccatt gatttttcaa gtcattagca ggagccatat ctaaataata
    11941 tttcccccct tctatcatct gatatagagc aaaaacagca ccttctaatg gttttccttt
    12001 ttcagttgtt cctctttgac gtccatactt gaagaagtat ggatctctta gataaccgac
    12061 atttttagga tagatatgaa tctcttgcag ttcctcggtt gattctgtcg ggtgataaat
    12121 tggcaaaata gcagcaattg ggatagctgt ctgatcgata tcaacatcca atccaacttc
    12181 tggatcaatc gattcttcga aaatcaaata aactgcatcc cgatcttggt ttttcttagg
    12241 tgctttgata cgtgcaatcc cgtctcctaa tccgccaacc gtatctttcc cagttgttac
    12301 cgaagtccct atttggacaa gcctttcttc tgtgattagc tgacggatat tgtttctgtt
    12361 cgtttcagcg atttgctgga cgaattcttt aggagtcatg ttttcagcag cttttttttc
    12421 atgataataa tcggtcatgt cgtaaatggc aaatgtcgct ccatttagtg gcaccgtctg
    12481 tgaaatgata tctgctgttt cccctgcttc ctcagagata gccaagccat cattttcatg
    12541 gtattcaaat tgatcgttat aatcgatatc tcgaatcatc cgtttatgca acacgaattc
    12601 cacgacatct tcccctgatt cttctgccga catcgcatgt ggaagaagga ctgctgcaag
    12661 cggtagtaaa acaagtaatt gaagcatcaa acacaacagc tttttcatta ccttgctccc
    12721 tcctttttag attgtctgac aaacaaacca taggctgctc ctgcaagcgc catgaagaat
    12781 gcagcaagca ggatatagaa taatcgtccg gatcctcctg tagcaggcaa cggattttca
    12841 ggatcgttcc cgacactaag ctgtaatctg attcgatttg tctccaaaag ttcatattct
    12901 aactcttgct gtagatcagg cgtctctgct tcttcgtttg gataatacga aatctcaact
    12961 tttagtgtac ccgcttcttt tcctggaact acccgatcat tttctatgtc aggttcttct
    13021 cgataactaa tttctaaaat aaagtaacct ggaagttttc tatagctgtc aggtgacttc
    13081 acttccttga tccgatagac cccttccaac aagtctgaag cattgaatag ggaagaatcg
    13141 tttggatctg gtgccaagcc tccctcgatg atttcatact ctcctgcact attttgtttc
    13201 tcaattgaaa attctgcgcc ttcaagacgt cggctattca tactgtcaat tttcaaaagt
    13261 tccaaatcga ttggcgctag ttcatcttct aaggttactt gtagtacacc atcgatgatt
    13321 tcatagcctt ccggcaaggg agcagtttcg tcttcgatga ccgtatcttc aatgataaac
    13381 ttaccttctt cattcagtgc aaactggaac acttgatcag atagccgata cccatttggt
    13441 gcgacagatt ccgagacctc atattcacca atagacagat gcagaggttg gtcgtaattt
    13501 aaatcgacat acttttctac tcctacggca cgagaataga agtatccgat cccaggcaaa
    13561 cgtttcgatt cttctggttc taacggatta ccagtgtatg ctcgatacag cgggaaggaa
    13621 gaatccccca ctttcctgat atcgaaacct gcaccatcta atgtatggcc ttcatggtct
    13681 aattttctaa ctcggatctc ccgataatca aacgtattgt aagcagtgaa tgctacccat
    13741 aaacgatcct cttcatctgt tgtatacgtg agatattgcc aattttcttc acctgtttgc
    13801 ttgagttgga tagtccctat agcattatcg taaagtcgca agatccatgt gtcattttca
    13861 tcaggttctt tgaaaccttc tgggttctgg ctctcaatga ttttgtattc tgcatagttg
    13921 gtagtttctt cactatcctt ataaacaaat gaatctggct ctccgattgg atgaataact
    13981 ttctcaccat ccacgattac atatttcccg tcttcatcta ctgaatagaa cgtgagtctc
    14041 cctttctctt cctctacttc gttcacacca gaaagccctt tcgctactgg atatccgatt
    14101 tccagtccgt taccatctgt ttcataaagt tcaaactctg cgccattgat tggcatatta
    14161 ctgccttcaa ttagtttatt gatattcatg tggattggct tgaattcatt tcgcatttcc
    14221 agtttgacat acaactcatc atactctgtg ccttctatgt taggctcttg tacaacagaa
    14281 agcttgtgat attctccttc ataataggaa ttcggatctt taccttccgg tccgccgact
    14341 ctcgaaccag atgtatattc atctacaagc tcgtagtgac ctaacatctc atcttcgttg
    14401 gctgcatatt ttttgatttg gaattgatac gtctcatcta ctaactgata acgtggtggt
    14461 ccagcgatct ctctgatcca aaatatgtcg ctgactgctc cagtttcatc tcctaaaatc
    14521 ggcatcttcg tttcaaagat cgctattccg tccgtggtag ttttacttgt ttcgattggt
    14581 tcggcttccc cactctctgg attttggtca tataatgcaa attcgactcc agccaacggc
    14641 tcgccatttt gacccttctt gctgacttgg aaattacggc ctttatgttc gtaacgaatg
    14701 gaagaagatg ggaaatacat ccgtccagta gtgatatcat tctcataatc ctccaggttc
    14761 tgaatatagg ccatattgtt cactgtgcaa aaatttccgt cttgagctcc cgcatcaagt
    14821 tttacttggt acttgatcct gaccatttcc ccttcaccta aagaaatcgg agaaaattta
    14881 taagcttggt tttcccgtcc gacatacgtt gtcggagtcg ccactacttc tggtggggct
    14941 tgtccatgac gttcttcatc ccatatttct ggctcttggt acttcatgcc gttttctcct
    15001 ggatctagat ggtaaaactg aatatctgcc gatctgccag ctccaaatgg tgagacatta
    15061 aaaccagttt catcataaag tgtggcttgc tggatcgttc tcttgaaggt agaagagata
    15121 tcctccaagg ctgtaacaat gtcattcttg ttgttcgcat tgtaatatcg cggtgttacg
    15181 ccatcttctt ctaaatcagc aatatttttc aatacatttc gtccaatagc atctagtcgt
    15241 tgtctcccgc taacgtttcc atcgattcct aaaccaatgg agaagatact tggcgggttc
    15301 tcttgatact tgtttttcag ccaatgatga taagcaactg tgttgatcgc agatgacttt
    15361 tcttgcgaag taggactgcc attcgtattg ttattagaag aaccatcacc aacccagtga
    15421 gtcgctttat tatctccttc ttttacctct ccataacgat agtgtacatt tacttcatct
    15481 tctggacggt taaagtcaga tgcataggta actggatgag taaaccctgc attagaagta
    15541 ttcccagaaa actcctcaaa atttctgaac aaaacgtcat tatcttcagc aatcttatct
    15601 tccatcacag tccagttgct ccaagctcca ttagtgctcg tacgataact tgactgaatc
    15661 ggtgcataac tgaaggtcgg tgtcccatct cccacaacga ttaggatttt ttctggattt
    15721 ctattttctc cgccattgtc tttatacaat gtttcatagc catttttaag ccctagtgta
    15781 agcggagttc ctccgattgg tgtacgattt aatgctgtat tctcacttcc cgtcaaacga
    15841 ttgatatcat ttgtcaatgg aaagatgtcc gaactgattt gctcatggtt attgatagct
    15901 gtactattat ggtaaaaatt caccataccg attcgaatat ccagactcgg gtcagagagc
    15961 agatctttac tcatttcgtt tactgcctca atcaacgcag catttttagt ttgactattg
    16021 gtcccagcgg ttaattcact catactcgct gacttatcta gaacaaaaac gatatcgatt
    16081 ggactgattt cctttttctc gcccccgatg acatctagat agacattgta aagggttgtt
    16141 gcatcttcag gatgtagatc agatgctgtg ctgcttggca gaacaggttc tacccatttt
    16201 ttgatgtacg ccccgttata atccaagtaa ctgttttcaa aattccgttc atttccccaa
    16261 agctgaacgg tttgagctgg aacgtttcta tcttcttcgc ctgcagttga tccattatta
    16321 gtagcatagt cgtaatttcg ccagttcgct cccccgtttg tataatcact atcatcgatc
    16381 aagttctctc cgtcatatgg cgagcctttg tctccttgat agcgttcatt gctttgtgtc
    16441 caatttccta acacttttgt atagtcattc gttgtaaact tcgggtagcg atgctgttcg
    16501 ttttcaactt ctccgctagg atagatatat tcataggggt cttgcgtagc tgttggccca
    16561 agacgctcca aaataccgtt gaataactgg cgttgaatgc gctggagtgc ttgaccgctg
    16621 ggtacgattg gttcgtccag cttttccatc tcattttctt cgctacgttg aatttcagcg
    16681 gaatcttctg tatcagtttc attttcttgt aattcttggt tatctattct ttcatcaacc
    16741 accacttgaa gaggtagtta cccttcgttc acatcgggga tcttttctgt ttcaaaaatc
    16801 aatgtgccgg tttctgcttc ttttgaataa acttttccga cataccattc ttgaccatcg
    16861 atcgtctcga cttttaggtc ttctccatct tcatcatagg aggatagacc tgttccacga
    16921 acgtttttta cggtaccaat cccggcggct gccgcatcga ttgctaaacg tattctacct
    16981 tcattggcac tttccttttt ttcaaaagcc agatgccatt caagggtttc ttctttttca
    17041 atatacttcc cttcaatttt tgcatagtcc tcatctaaga atacctcatc ctcctttgtt
    17101 tctgttgact cagcagctac atccagggtc gtcatatgat agactgactg gaaatatggt
    17161 ccaaatagca aactgacagt tactaactgc aaaagcttca attttaaccc tttcacatcc
    17221 ttatactcct ttcaaaaata tttgtcttct ttcacaacaa tcaacaaaac aatgcccatc
    17281 taattataat ttaatttaat tttaatttta aatcaaatga tatccttttt agttaaaaaa
    17341 caccaattat acaacattta ttattaaaat taaattataa aattttataa cgttctcatt
    17401 taacaaaact taagttatta agacaaatga ataaacaact acgctttcct cgaaacaaaa
    17461 aaataaagca caaatgcgca tttacgcact catgctttat tcgtacaatt aattcagata
    17521 acggcagcgc ataggatgcc aataacactt cctaaaatag ctcctcctaa aatgtcagta
    17581 ggataatgta aaagtaaaaa aatcctcgaa taagcgatca aacaagcaaa taaaaaggct
    17641 aatattcctg caactggact taattcgaac aacgcagcta ctgataaaaa ggctagcgct
    17701 gtatgtcctg aaggaaaaga agaagtcttc ggtttacttg tgtaaaggac aagccgacga
    17761 tttttttcat aaggtctttt ccgtcttgtc gtttttttga taaaaagcaa accaacaaaa
    17821 aaatagatca aactgccgat aagaatggct agaccatatt ctctagtatc aggtatcaag
    17881 actaaaacag cagcaaggag caaccaaata aaaccaccat tagagatatt actgataaat
    17941 ttcaaaaaat tgatttttcg actatccgtt ttccgttgct tggcgaactt gttgataata
    18001 ctatcatcga ttttagcaat caaacgcaac atgttattcc tcctttttaa gaaccttttc
    18061 ttgcgtacga tggataaaaa gcaggacttg aaaataaaac aagaaactga aacctaataa
    18121 agcaaaatag attggtcctg caaaccagaa tgcgacaaga aggatcaacg tgcctgccaa
    18181 atagaaaagc gcgcttttga aatcattaaa aaacaagaaa aatgcagtaa tccaagaagc
    18241 ttctttttgt tccagttgaa gcaagatttt gtaactaaag agaagcgcgc ttaatagaag
    18301 caaaaacaac gtagtataca tcaatgtata gccaattccc ccaccccaat agcggaaaat
    18361 ctgataatcg atcaatagaa gaagaaaata aacacttgct aaactatctt gctttccata
    18421 aagagagaag ttctcacgaa actttttcca gaatagtgaa ccgagctttt tctcttcatt
    18481 ttttttgtaa tgataaatca tatacagaac tgttcgactt gcagttccta atgtgaaaac
    18541 aggcaagacg cacaggaacc atagtagctg taaatggata agacgtggaa tccattcaat
    18601 gatttctaag aaataatcat tggctttttt accaatcgat cgattcatca tccttttgaa
    18661 cctcctgtca aatccatccc ttcaatgaaa taacgttgtg caaaaaagaa cagcaaaatc
    18721 gtcgggatca agacaagtgt tgctcctgcc atcagataat tccattgtgt cgttgcttgg
    18781 ttctgaaaaa cttgcaagcc aatctgtaac gtgtattttt cttgatcttg gatatataac
    18841 agaggaccta agaaatcatt ccaagctgcg ttgaaggaat tgatcgcaat ggtaatcaag
    18901 gcaggtttcg tcaatggaag catcaaatga ctccaaatat acaaatgatt cgctccgtcg
    18961 atttcagcag cttcgatcaa ttcattattg atcgataagt aaaattgacg catcagaaat
    19021 atattgaacg cattgccgaa gaacgatgga acgatcaggg gcaaataagt acctacccag
    19081 ccgatcttgg agaataatac atattgaggg atcatcgtta caaaccccgg gatcatcatc
    19141 gtagaaagaa ccaaagcaaa catcagcttt ttcccaggaa aatcaagctt cgcaaatcca
    19201 taagcaataa aagaatttga aaggacatta ccgatcacta ctagcacagt gatgaataat
    19261 gtatttcttg catagcgcca aaaaggaaag gcggcaatcg tatccagata gttttcccat
    19321 acgatttttt ctgggaaaaa ggtcggagga taacgaacga tttcttccat tggtttcaat
    19381 gaagtaaaca ccatccaaag caatggaatg atcaaaatga ttcctattac tgtcatgagg
    19441 acataactta gtattttttt tgtttgccga ttcacactgg tctttttgaa ataatccatc
    19501 atttgccgtc acctccagaa taatagaccc acttaggtgc aagcttcaaa ttgacaaacg
    19561 tcagaaccaa cacgatcaga aacaggatca ttgacatagc catcgcataa cccatatcaa
    19621 aagctacgaa cgccttgttc cacatatgca gattataaaa aagcaaagaa tttgcaggtc
    19681 cgcctgttcc atttttcgtc atgacataag cttcttgaaa aatttggaaa gaaccgatcg
    19741 tagaggtgat cacatcgtaa aaaataatag gagtgattaa tggcaaagta atacttttga
    19801 actttcccca aacgcctgct ccatcgattt ctgctgattc atataaagat ctaggaacat
    19861 tttgtaatgt agctaagtaa agtagcattg ctcctccaga actccatatt ttcatcaaaa
    19921 tcaacgcagg tttcgtccat tgtggatcaa acagccaagc tggtccaggg atatggaacc
    19981 agcccagaaa tgtgttgatc aatcctgcag atggagaaag cagctgcatc cataagatgt
    20041 agactgctac tcctgataag actgccggta aatagaaagc tgtacggaat acactgattc
    20101 ctttgatttt ttgattgagt actgtggcta aaaacacact gaaaatcgtc gtcagcggta
    20161 cattgaagat gacgaaataa acggtgttgt ataacgattt ccaaaataaa tcatcgtgaa
    20221 cgaacattct tttgaaattt gctagaccaa tgaaattcat cttggatgtc aaattataat
    20281 cggtgaaact accaataact gaaaaaagca taggccctag cataaatacc aagaacccga
    20341 taataaaagg cgagataaat aaataggccc atcttgcttc ttttttcttt cggatagata
    20401 aacgcttcaa attttcttgc ctcctctgta cctagacagt caaaaatgga ttattgattt
    20461 tgcttgacta agttttctac tgctttttgt gcatctgcca atccttcttt tggtgatttt
    20521 gttcctaaaa gaatctggtc aatctgttca ttcactaatg tcgtgtaatc cggagcatct
    20581 aaaggaactg gtgtgatcac cgtattttta aagttttcgt ccgccatttg atagatcatt
    20641 tttcctttat ccgttaggtt gtcattattc actaaattct cattggctgt cttgctcgcc
    20701 atgatatcaa aacttttttc gccgaatttt tcttgtactt ctggagaaga aagatatttg
    20761 atgaattcat aagaggcctc aggatctttt gctccgtatg gtacttccaa gacgaatccg
    20821 ccaccccacg accaatggcc tgaaccttct tctttttctg ggagctgcat cacgccaaaa
    20881 tcaaaatcat ccggtgcatt ttccatcaaa ctagagtaat aattgatatt ttgggcacgc
    20941 atggctacta atccggaaat aaatggatca gatacaccag acccaaattc ggcttctaat
    21001 ttattgatcg tatttttccc ataataatct tgccagtctt ggatccactc caaggcttcc
    21061 actttatttt tagtatcaat cttgactttt tcatctttgt caaaccaact agtcccgtta
    21121 tctgcgttta gcgcccatac atcagcccct aaattccata atggataaaa accgatgcgt
    21181 gtaaattctt ttccttcttt tttatctagt ttacgtgctg tttcttctaa ttcatcccaa
    21241 gttgtcggta gatcttgttc actgattcca gcttctttaa ataacttttt attataaaaa
    21301 atgacttgtg tatctgtatt gaatgggatc gcataaggat caccttcata ttctactgta
    21361 tcccaaagct gcggataaaa atccttagaa gtgtcttttt ctacatattt ggagagatta
    21421 gttgcttgct gtgcttctgc tctttgggca acggaattta tgtcctgtac aataacatcg
    21481 ggtgcatttc cagctgttat tgcagataaa gatttagtcc agatgtcacc ccatggctga
    21541 tacttataat ttacatggat ctcgttttgt gattgattga aatcctcaat gatttcttcg
    21601 atagcttctt ttcgtgcacc ggatccccaa aaggaccaga aatcaatatt ttttcttcca
    21661 tcacttgttt tttcagaaga atttgctgca cttgaactgg catcgcttgc attacttgat
    21721 ccgcatcctc ctaataaaaa caatgctgaa cttgccagca accctgtaaa caataatttt
    21781 ttcatatttt acctccaaat tttaatgtaa ccgatgccgg ttttaatata acgatatcat
    21841 tttgttctga aaataacaat ttataaggta tgattctaaa aaaattatta atgataccaa
    21901 tactttatat aattcgcgag cgctttcttt gaatttttta atctttcgta gcagaatgag
    21961 aatgtattaa aaatagatta ggaggaattt tgatgtttag acaccagaaa gaattgcagt
    22021 ttgaagcaaa accagatcgt ccgaatccat taatcgcgaa atatctgcag gagctgatcg
    22081 gcggccaata tggcgagatg agtgtagcta tgcagtacct gttccaaggc tggagctgcc
    22141 gaggagaaga aaaatataaa gatatgctga tggacattgc aacagaagaa atcggccacg
    22201 tggaaatgtt ggcgacaatg gttgctcgtt tattagaggg tgcgcctgtt gaagatcaag
    22261 aagcagcgat gcaaaaagat ccaacaatcg gtgccattat ctctggaatg aatccacagc
    22321 acgcgatcgt agatggctta ggacctcgtc ctgctgacag cgtgggcaat ccatggagcg
    22381 gtgcctatat cattgcgagt ggcaacttgt tagcagactt tagagctaat ttaaatgccg
    22441 aatcacaagg aagacttcaa gtagcacgta tttacgaaat gatcgatgat cgtggcgtaa
    22501 aagatatgct gtctgttctc cttgcaagag atagctatca tcaaaatcta tgggaatgtg
    22561 cagtgaaaga attagaagaa aaagaaggtt caatcttagt cccaagtact ttcccgcgtg
    22621 acaaagaacg aaaagacatt gcttatgact tctataactt ctcagaggga gaccaaagta
    22681 gtgaaggttc ttgggctagt ggcaaagcac tggatggcga aggcgaatat caatggttga
    22741 aagaacctaa gatcgaaggc gctgcaccaa aattagaacc agtaacacca aaaatgtatg
    22801 ggacacctcc attcaagaaa taaatatcta cccgtagaaa cacccgtttt ttgttccaca
    22861 cgctttgtga gcaaaactga ctaaagtcat taatcataac cacccttcaa aagggtggtt
    22921 ttctctagag ctataagctc aaatcaccgg ccagcgccta aagacgctgg ctttcatatt
    22981 ttatgttcaa gccatattgc ctttgccagt tttgcccacc tcttaaaagg tttatgtact
    23041 actccttagc ccttaaaagg atcgtcttac tctcgtacac tcagcttatc taacgcacta
    23101 tcatgttttt cttgttcttg aatgtacttt ttgatggtcg cttcattttg tcctactttt
    23161 gtcacatagt atccttccac ccaaaagtgt cgatacccaa atttatattt tagatttgca
    23221 caatctcttg atgatctcct gaatattggc tcgatattga ttataaatta tttttcttct
    23281 atactttggt gtgaatacaa tgtggtattt acacatctac tttgtacggg ctaaactatt
    23341 tccctttatt gctccacccg catagcaagt ggttttgtat ttcaatcgct cggcgcgctc
    23401 aattaggtct aaagacaata aatctaaaaa cccgctgctt cactgacaga aacaacgggt
    23461 tttcagaaaa aaatgaaatg aaaaagatct cttacggcaa tctattttat ttttttgatt
    23521 ttttataaat ggaaaatgct tcaagaagtc caagaaataa agccgtataa aaacaagaac
    23581 ctatgaaatc tttatttatt agatattcaa cagaaatccc gataacacta gccaacaata
    23641 tatttatcag aagtgtaaaa taaggatgct ttttatagaa tagaactagt gttgttttca
    23701 tagactctcc tttcttcata tttccaatta atcacaaatt cacggcatta ctagaaaaat
    23761 taaaggggcg cagaagtgga aaaatactct tcgactttcc ctacaaacca ggccatcttg
    23821 tgtttaataa ctataccaaa tctaaggtac ctctaaatct aaattccatt tgtggcggtg
    23881 acctccaatt tttaactctt ctatctccca tccagtatac ataaccttga tacttcgacc
    23941 acttatatat cgaacacaca aatacctatc cattgcagga gtactaccta acttagttgc
    24001 tacttgcatc taaataacag ctgaaaagat aggacttact cttagtaaag tatctgaact
    24061 attaattttt aaactatcat ttttatcctc aactacatgc acatctgttg caacttcact
    24121 ttctgtctca tccagataaa tgattaagga atcgggaatt gcaatatctt cagctgacgc
    24181 tggaatagtt gagctaaatt agtcatcata tctttattat aaaataacca acagcaaaaa
    24241 atacaatgaa aaacaaactt ggaactaatg tatcattaat aaatgttgaa aaattcagca
    24301 actcattact gtatgatttt agcagcccaa ccaacatata ccctataaaa gctaataaga
    24361 tggttaggaa tcctttagaa tttttactca ttcatagaca cttccttaaa taacttttat
    24421 tatagaaggt attgtaaata ataatattga caaaaagtga ctcaaaataa aaaaaacctt
    24481 acattatact ggaatgaact gtattgatag tgtacctaaa agctctactg acataagatc
    24541 gtgtaatcgg taaagtaatt ttattaatta tacgggatgt aatcaattag agaataaact
    24601 aatcataaaa ggtactttta cattcataat tgtcgctttg agattctact ttggttcact
    24661 atctgaccca attgcacatt ttcgatcatt acaagtattg gtgtcgttag aacaactaaa
    24721 taaaaactag cggtagattt ttcagaatct atgctaattt ctgtatggat gttccagtga
    24781 tggcaaattt ctcttttcat tgtttttctt gtcgcatgat atgccttttt gtgttattca
    24841 gttaaactat agaaattctt tcctagacag atcttgatcc caaattttac gtttttgagc
    24901 atcgatatag tttaaaatga cattactcat atctaaagag tttagatttt ttaggactaa
    24961 aactgtcagt tgttcttttt cttctttctg ttaaagtatt gtcgcgactt ttcgtatacg
    25021 ttaagatatg tcgtgaaaaa acaagttttg tgacacaaaa aaacgaatga cttgaggaaa
    25081 tcacctcaat cattcgttta aaatgctata tattctttag tttaagatac ggaaattaat
    25141 gccatcgacc acactagtag aatgaacgac atttacttgg ccttttttta atttcgttat
    25201 gtttagcata taaaaatatt atagagttta cttacaatgc ttaaaacata tactgtataa
    25261 tctgatcaat tttagccatg tcttgcaatg gtaagttttc tatttttttg agttttcgtt
    25321 ccttaaaatc aagagatttt aattgtgtaa taattacttg tccttttata tctagctctt
    25381 caggtaatga gtaacgtgtc ggtaaattct ttatagtgga agtaattgga catatgaccg
    25441 aaaacattgt tttacggtta aattcataac gagagacgac tagtcctggt cgtctttttt
    25501 gaatttcttt tcctacggag ggatcaaaat caatccatac aatatcacct tttttaggaa
    25561 tataaattct ttcttctgac actataaaat ttctctccct tcaggagtta aatcttccca
    25621 ttcgtctttc tcataatact cggcctcttc cccaccacta aatggatctt caatttttgg
    25681 aactaacgta attgttccat catcagaata cacaacgata tattcttggt tttctgaagg
    25741 tttctttccg ttatcagaag gtaaagttac cactacagaa cttccttgta atctagattt
    25801 tgtaacaagc attatcatca ctcccttaat aaaagtatac tatataacac taagtaacgc
    25861 aaaattcccc tttgtaaact atctagaaaa ttgttttgtt taaagtatat cttattggat
    25921 ataaatagaa ataaattgta gccagttcat tttttcttta actaaaaaaa taatccaaga
    25981 aacattgaac ctatggatcc aaaagctacc caaatactat agctaacccc ccgctacctt
    26041 caattttgca gataaaacgc tttgggagaa aatttcaaag acaacaggcc atcctatcat
    26101 gcccgattat aaacaaaaag ctggctattc cttttgctct gacaaatttc aattgggaaa
    26161 gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg atcaaagaat gcccgcttca
    26221 aatagaaaca ttggttcaac gaatttcaac tagacaagac tttgctattg tagaacgtga
    26281 gatcgtagct atttttgtag aagaagggat tttacatgat ccaactcata tcaatgtgga
    26341 taaatggcaa cctcttatct ataaattcag agaatataca actgccaacc agtcgttaac
    26401 tttgaatttt agattccaag aattcaaaga gtcataatga taaacatcgt taggtataaa
    26461 taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc tatacattag aaaggtcgac
    26521 tgtgacccta tagatgcagt atatgcagct tatgagcatt tcttctgttc tctttgtttt
    26581 tgggatctgt gctgaagtct ttttcgtttt ttgccgctct ttctttgaaa aatcgattat
    26641 gttatacctt ctccattact aattcaggca tctgctgagt aatgattttt cgcgcaaaag
    26701 aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa gacaagcggc attcggtcat
    26761 ggattggtgc gactgtgcgg ttaggttcag tagtcattat gattgattct gccatttttt
    26821 catcctgatg gctacgataa aaaccgccaa tatacactat tttttcctta tttgaaaaca
    26881 taaatttgtc tttttcttca ttccactcat tgggaaaaca caacgatttt gcaaaaatgg
    26941 ttgatgaaag gtcttttttt cttctactgt ctctgcacgg gcattaatca tcaattgtcc
    27001 ttttttaaat ccggtaaatc cccacttcat gatttccgga gtgaggatgt tttcttcctc
    27061 ttttcttaac gtcacaacaa gatttgacgg aaaaacttct ccagctgcta attctgctgc
    27121 ctgcttcggt tgtttcgtct gggcttctcg ataataattc tgaaggagct ctgactctag
    27181 atcaaaaaaa taacgaccac acatcctaat ccctcccaat ctttttttct atcatacact
    27241 gcaacaagaa gttcgcacca acaaaacgct ttcaattttt gtagaactaa aaaagttgtg
    27301 acaagtgaca aactctccag tgaatggtat tctcctgcca actcctcggt cacaagtcac
    27361 aatattcaac aaacatgaga agctgctttt tgaaattgtt ccttgtgact cggagtgaaa
    27421 cggcaggctt acgacctctg ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc
    27481 aaatttatgc aaaaatgtag aaagctattt tcacaaaatt tttcttaata ctcgaaacta
    27541 agcgactttt tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata
    27601 agcccaacca aacgaaaaat atgagcagct atttttattt ggctgttctt attacttgat
    27661 gcagggcagc tttttcacaa cctctccact aaacggtgtt caatcagcta accttcggta
    27721 ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc gtttgtttct ctaatactca
    27781 ggtcaaagcg ctgatttcac aacctctaat acacggtgtt ccagaagtag cttcttcgaa
    27841 aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
    27901 ttgaagctga ccacttctgt cacaacctct aattattaaa gaaccttttg cgaagttaac
    27961 aatctttctt tgatcaattg aaaattctgc ctaagtagtg atagatcctg tgtgactttc
    28021 aacaataaga tacttccctg gatttcagca atcaacgctt tggcttgacg gtctgcttct
    28081 tcatcggaca gtttttcttc agaaagtgcc aggatatttt gtttcgtata cttgacccat
    28141 tgattgacta attctagtag caacagacgg tattcttcat ccactgtact caactcaata
    28201 atcaaattac ctacaggaca gccatgataa gtgatctgtg tctcatgatc agcgaaaatc
    28261 cagtccaaca tttcttctaa tgcttcttct ggctgttttc cagcatttaa gatgtcttcg
    28321 aacaattctg tctgccatac gtccatatgc gcatggatca ccgctagaca aacttctttt
    28381 tttgaagaaa agtaataata aaattgacct tttcctgtat cagaagctgc tagaatatct
    28441 cggattgacg tttcatgata gcctttttta taaataagat ctttggtcgc ttccaagatc
    28501 ttttcctttg ttttcaatga tattctcctt tttcatttct catagtccta tattatacag
    28561 taaattcatc attttttctt cttaatcgtt taaaaaagac tgtgccaaat tatctgacac
    28621 agccctcact ataaaactgt tctcatatag ctagatgttc attgattcaa gaactgcgag
    28681 gacaaaagca attcctatct tatgatagtt gctctaatac ttcctctgtt gtcatcactt
    28741 gatctgcaat aaagccaaag ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac
    28801 caattgcatc tgtcgctacc acaacattga atccactctc taccaattca cgcatatggg
    28861 aatcgctaca caagtttccg ttcattccac caagaattac tgtgtcgatt ccattttttc
    28921 tcaattggag cgccaaatca ttgctttctg gtccaaagat tttatgagga gagctgatga
    28981 tcgtattttc atccaaataa ggtttcaatt cttcgataaa atcggctcct gaaccttctt
    29041 ccactgattc atattgactt tttctagcaa acatctgctg ctcaagcatc atatgttctc
    29101 ctgcaccgcc aaattgccat ttatggtcat gtgggtaaaa ataatgagga gaaataaaga
    29161 gtttgtatcc tttttctttt gcttgtttta ccatttcaat catattttcg atcgtattgt
    29221 atttttctaa gatatctttt gtcaaaccaa agccaccgcc tgctggtttc aagaactcga
    29281 cttgcgggtc agtgagaact actgctgtat ttttattatt gaacatggtg gattctccat
    29341 tcgttattta ttttaataca tcatttagag cttcactcat tgtcggatga gaatagattt
    29401 gatctctcaa taacgtataa ggaagttttc cattcatagc tagcgaaatc aagttgatga
    29461 cttcatgaga atcttccgca tagatacttg cccctagaat caagtcagtt tcagggtcga
    29521 ctaaaacttt gaatacacct ttaggatctt ctaatacttg tgctttaggg acaccgcttg
    29581 tcatatactt gaataatttg taattgacgc cttgtgcttt ggcttgtttt tctgttaaac
    29641 ctacattgga taaagtcgga gtgataaata cactgtaagg cacagctttc cggttattcg
    29701 ttgttcgttt attctcgccc ttcaactgat ccataatgat tcgataatca tctagagaga
    29761 tataagtgaa ctgcaatccg cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg
    29821 tacgaaggta ttcatccaca gcgattgctc cgcgatctgt tagtttaaca tctgtatttt
    29881 ctaatccaag tgatgctgta cttggttttc ttccagttgc agccaaaact tttgacgctt
    29941 ggattgttac ttcttggcca tcttctgtaa aggtgacggc agctgttgat tcttggtctg
    30001 ctacttgatc tacagataca cctagatgga attcgattcc cgcatttgtc atgtcttcta
    30061 aaatcatttg cgcgatatct tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa
    30121 cgacaacttt agatccgtat tcattgaaca ttgatgcaaa ttccaagcca atatatccag
    30181 ccccgatgat cactaatttt tccggtaatt gttcctgatc catcgcttgc gtagaatcaa
    30241 tcaagtagcg acttgtattc aatcctttga ttggtaaaat gattggttca gaaccagtat
    30301 tgatgaaaat ccgttcgcct tcaacaatca ctttttcacc attattcgtc acttcgattt
    30361 gatggttaga aagaaaacgt gctgtaccgt ccatcactgt cccagttgcc tcatcgctga
    30421 tcatatgata atttttggcg cgaagcatgc ccgtcaattt ttctttccga gcaacagcct
    30481 ctgtaaagtc gattcctctt tctccattga aaatcaatga tttagaagga atacagccga
    30541 tgttgataca tgttccgcca tacatacgtg ttgacttttc aacgactaaa accgattcac
    30601 cttttgaagc taacgccttc gctaaagtct taccgccctt tccaaaaccg atcacaatat
    30661 tttgatattt ttccatcatt tttccctcgc ttctttttta cctaccgatt ggtacaaaaa
    30721 tatgataacc gataattgtc tattctccaa atcatctgct ttatttgtca aaaaaaacca
    30781 gacaaaaagc ggtcctttca cgtttttagt ctggttcgat ttcctttatt cttctaaaca
    30841 atcgttactt ctacatcgat atttcctcta gttgctttgg agtaagggca gaattcatgc
    30901 gcatcttctg caattttttg ggcttcttct tcgctgatgc catcgatatg cacttctaat
    30961 actacaccga tctgatagcc gccatcatct aaattataaa gagagacacg tgcttttact
    31021 gtggactctg ctgtcattcc tgcttctttg atcatatgtt gtaccgcccc attgaaacaa
    31081 gaagcataac ctgctgcaaa taattgttct ggatttgttc cttctgcatg gattcctgga
    31141 ggagtgattt tcatatgcat cgagccattt ggtgcctcga cttctccatt gcgcccgcca
    31201 tggttgatca ttgctgtttc gtatgctttt ttcattgtac tgctcctttc gattgaccat
    31261 ttgctacttg gctataatta tagtgtatga caagtaaggc gctttctaca aaaattctgc
    31321 ttattcgaat accagaatgc cgtcttctac tatttttcca aaaaacaaaa aaatactact
    31381 cgtgaacaac agcgtcatat ttgttccaaa ataaatagca atcttttcta cataatcttt
    31441 tatatgattg attttaagat acatatatac cagaaagttg accccataaa ccaatactgt
    31501 taaaaaaata accatattcg ttaccatttt ttcctccttt tgctggtctc cctaccagca
    31561 ctcgatttaa ccataccact ttttttgccg tccaagcaac tagcctttca aaaagactgg
    31621 tttattctgc tgttaatgat tcaagatcag aaaaatgtcc gaataatttt gctaatttta
    31681 ctaacgccaa cttgttcttt ccgaattcat cattttctat ctgtaatact agtaacggct
    31741 catgtaagct taaacggatc aaaaaccagc cgtttccgta tggctcttct agatcatatc
    31801 gaataccttc ttcattttcc gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct
    31861 tcatttcttc taatatcttg tttccttgtt cgcgtggttc atcgctgaac aattttaatc
    31921 ggatttcttg tacttctacg ggctgtttca actctttgat ttgttcatct aatgtgatac
    31981 cttttctttt taattctggc aaaagcatca ggatctttgc gatgacatat gcaccatcat
    32041 ccagaaaata gttttccttg aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat
    32101 ctttcccaag acggttaagt tctaatgctt tattgattac atttcgatag ccggaaatat
    32161 aacgaacttg gatgcctcct ttttcttcaa tgaatctttg tagatgagaa gaagtagggg
    32221 aattcgtcac gattcttgtt cctggatgct cttttaatac gattgtactt aatactgcga
    32281 tcaaattatt tcgattgatc actgttccag aagcactgac aactgcagaa cgatccacat
    32341 ctgtatcgaa aatgatccca agatctgctt catttgctaa aacagcctgc ttgatacttt
    32401 ccatcgcttc tttattatca ggatttggga tatgattagg aaatcggcca tctggttcca
    32461 aaaattggga acctgttgaa tccgcaccta agctattcaa tactttttca gcaaagaatc
    32521 caccagcacc atttccggca tcaacaatga ttttccaacc atttagtggt ttgctttcta
    32581 gtgttccaat ccccttttta atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt
    32641 ctgctttttc tacgcttcca gtttgttcgc tttttatttt ttgctgctgt gataggataa
    32701 aagcaatatc ttctttttct gctcctcctt ctgaggtaaa gaattttatt ccattataat
    32761 aatagggaag atggctagca gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt
    32821 gagtggacat aaacatagca ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt
    32881 gttctttcag actttggatc aacgcctctt tcagtgctgg tccgcttatc ctactgtcat
    32941 gaccaatccc aatcgtccat tttttatttg gatcggttga tttcttcttt ttctttatcc
    33001 agtttgctag tccccagccg atctttttta ccatgtctgt cgtcaagttt acttcatatc
    33061 cttcaactgg taatgcgatt cctcgaatat ccgatccatt ttgcagcgct ttcatatctt
    33121 tcatataaaa gtcctcctca tttattccct cattttttcc agcttttctg caaatcgctg
    33181 tgcttcttct tctgagcggc aaataataat gaatgtatct acaccggcca tcgttccgac
    33241 aacttcaggg taagccactt catctagcca attcgttacc acatcggcac catttcgttc
    33301 agtcaatacg ataaccatga actgaacaga ctggatacgc aaaacctcgc gtctaattgc
    33361 tgtacgcaaa cgttcttcat ttaaagaaac agatggctgg ttaaataatg tatagcgtac
    33421 tattttgttt tcattttgac tttttactaa cttcatttct cgaatgtctc gagaaatcgt
    33481 tgcttgagtc acatcggcgc ctgcttcatg cagccgcgca agcagctctt cctgtgtccc
    33541 aataggttgt tctgtaatga tctggcggat cagtgcttgt cgctgtttct ttttcatatt
    33601 agacacctct caatgttgat tatagcaaaa gataagtaat atttcttcat attttggtat
    33661 tttttacttt tattttttgt tttcattcag gttgctaacc tttttctatt ttaacacgca
    33721 aaatcgtctt cattttttca ggtgctgatt ttcttggatc gctgatgtaa atctctttgt
    33781 gttctttaga agctctttca tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg
    33841 ttgctggttc ttcggcatag ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca
    33901 tctttttgaa tactacttgt tctttcaagt cttccggaag tttgctagaa cgctgaatgg
    33961 cttcttgtgc tatcgcttct gtaacaaagt caggctgtcg gatcatcaat tgataagaaa
    34021 aatgttcttt tttcatcact ggttcatgca aatacttttc ttgcagtccc cattgtcctt
    34081 ccagtggata gacagtatac atctgataat caggaatcag ccaatttttt ttaggcgaca
    34141 tgcgtattgc atagctgact gcatatagac aggctactct tcgtttaaag tcttcctgat
    34201 tcgggtcgcc gatccctctg atgctaaaga atgatttttt aggaacctct agtatttgcg
    34261 ggcttttttt catactatag ctcttgtctt tacgccattc aattttttcc atttttcttc
    34321 ctccgcgaaa ttccatttag ttatttatta tcatacctct ttttgagtga cttggctaat
    34381 cgtgttgaca ctatttttca gtttgtgcta gaataagtga agtaaataaa tcgtacagat
    34441 aacacgtttc ggcgtgtcga gagagaggcg gtttctattt ttataataga aatcactctc
    34501 tttttttacg aaatttttta gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag
    34561 tacctgcagt aattgcaaca gggatgatgt cttttgcggg cgtattgatc gagacggcca
    34621 tgaatgtaac tttcccaact cttatcagac agtttgattt aacgactgca caagtccaat
    34681 gggtaacaac gatttatctt ttgatgattt cgatcattgt tccgctgtct acttatttga
    34741 atcgcaattt tagtctcaaa aaactctttt tagcttcaaa tctgcttttc ttggcaggta
    34801 taatgatcga tttcttctct cctaccttca tggtcctatt gtttggtcga ttgctccaag
    34861 gtgcagcgac aggtatcgct cttccgttga tgttccatat tatcttgaca tttgcccccc
    34921 tcaatcgacg aggcgcgatg atgggtgtcg gtactttgac aacggccatt gctccagcaa
    34981 ttggccccac ttatggaggc attatgactt cacattttac atggaatcat atcttcctgt
    35041 ttcttgtgcc cgttttgttt ctttctttga ttatcggctg gtttgctatt cccgagatgg
    35101 cagtacaaaa aagtggttat ttggattgga taagcgtttt aggaatttgt cttttgttta
    35161 gtggaagtct aacatttttg agtacgttgg gaacagtttc cagctggatt gctttaatca
    35221 tcggcggtat tggctggttc cttttttact atcgaagtaa aaaagtggag catccactga
    35281 tccgactgtc tattctttca aataaaacat tccgcttgtt tttgattggc ttcttagttt
    35341 atcagttttt attacttggt gtatcttttg tcttgccgaa tttccttcag atcgttcaag
    35401 gaagtagtcc ttttgtcgct ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc
    35461 tttctccttt ttccgggaaa atgctggatc aatatggtcc gaagaagcca atttttgctg
    35521 ggctgattct ttctttgatt ggttggctag cattattcct actcatagga cacgcttcag
    35581 tgggtgtatt gattgcttgt catgtgtttt atatgatcgg tatcggatta tcttacagca
    35641 atatgatgac caccggaatg aatgcactga atgaagaact acaaggagac gggaacgcaa
    35701 tgttcaatac cttgcagcag ttttctggcg ctgtggctac ttcattagta gcggtcatta
    35761 tcaattacgt ccagcatcat acttcccata actatgaagt gtctactaca ttaggatcaa
    35821 aagcagcatt aggcgtcttg ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt
    35881 atctgttctt tgctaaaaaa gcgtagattc catatacggc aggtgttgca tctgccgttt
    35941 ttttgtgtga aaatggttgt ttgtaaaagg aggctttttt atggaaaaaa ttgccattat
    36001 cggtggcggg atcattggta tgaccctagc cagttactta gatacatcaa aattcaatgt
    36061 tgctttattt gatgaaggga caggacaggc aactaaagct agcgctggga ttatctcccc
    36121 ttggttgtcg aaacgcagaa acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt
    36181 tttccctaaa cttataaaag atttccaact tgatcaaacg atttacgcac aatcagggac
    36241 acttttgctt cgacctgaaa aagatttagc tgaattggct gttcttgccg agaaacgaaa
    36301 agaagaagcg ccagaaattg gaaacatcga atggctttca cctgaacaga catctcaagc
    36361 acttccgtta ttgaaaccag cagtttcact gcgtatcagt ggtggtggtc gtctggatgg
    36421 aaaagcatat ttagaacatc ttggtcagcg tttagcacaa aaacaaatgg agcagatcca
    36481 ggcaaaagtc actttgcata agaagagcga ttcttggctc atcaagggac cgtttggaga
    36541 atacacagct gatcgtgtaa ttttgacacc tggcccggca cttaagcctt tattacaaga
    36601 attaggctat gctgcagata tacgtccgca aaaagggcag ctcttagcat ttcagacatc
    36661 ttataagatg agtcaagact ggcctgttgc tatgcttgac ggagaagctg atttcattcc
    36721 tttcatggat ggaaagatcc ttttaggtgc tacccatgaa aacgatcaag gctgggattt
    36781 ggctcctact agcgaagctt ataagggatt gactaagagt acagaacagt tcttgactga
    36841 tccagatcag ataaaaaact ggccatacga gttccgtgta ggtacccgtg cttatacgtc
    36901 agatttttcc ccatttttcg gcgtactgcc agatgatcca agcttactgg ctgctagcgg
    36961 tctaggctct tctggcttga ccacaggtcc ttatatcggc tatctattag ccaacttctt
    37021 aaatactggt cattggccta cggaagatta tcaaaaggac atctccacct atattacgaa
    37081 aaatccatca tagaaaaaca gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga
    37141 ttacttcttg aaaaagctgt tattttagtt caatttctga atcaaattct ccaaactttc
    37201 ttttgcttca tctggcaaga gatcttttcc tatctgagct tcaatgaatc gtaaacgaat
    37261 atcaagtcgt tgtttcacca gatttttata ggacgtatcc tcaaaagaaa caggaaaatc
    37321 tggtataggt aaatagtgga tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg
    37381 ttgcacgatt ctttcaacga ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa
    37441 atatccagta ttcaggacat agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc
    37501 ttgatagtct tccctaagag gatagacacg gaaaggatta gcaaatggtt caatgactag
    37561 ctcatctagc tgtctttttg tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt
    37621 tgcaccaaaa gacgctgcta aaactgggtc attgatcttg ataattggcg gaagcgaatc
    37681 atctttcatg atccaaaaaa cagcatcgat cttttcgcta agatgatcga ctcggtcggg
    37741 tgtcgcataa cgtgatttga ctgtccgccc atttccgttt cgtatatctc ctgttactag
    37801 gactttttct cctttacttg tacatgtcac gcccacattc tgacaagtaa ggaaagaaga
    37861 aatggccgga tcatcagacg gatagtcttg tgttttatca aagtaagaag gttccaaggc
    37921 agtagcaaaa ccattttttc gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga
    37981 gtgaccttcc gtatgttttg ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa
    38041 tacagccatc gtataggtct ggtcattact agcatattgt ttcatcccac cgtggcaggc
    38101 aataaagcca ttgcgatttc ccaatgccca agcaagcgtc aaagtcgctt tttttaattc
    38161 tccaaagtaa cgtaatccta gcacagccgc tgtattatgg agagcatcaa acaaaatgag
    38221 tccatttgga aatgtttcat gtgtccattc gggatcacaa aagatataaa gatctccctc
    38281 atcaaaggaa acagactttt gataagtctc taagatcttc gaattcgctg gctgaaaatt
    38341 cagcaaataa gaatacaaat tgttctcaaa tccttctgga atcagcaaat gactttgcac
    38401 agaaaattct tcagataggc caacgattac ttttgtatgg taaaactggc gcttgcgact
    38461 ttgatacaca gcttctctta aaattttctc gtatttgctt tgttccttgt ctaagtcctc
    38521 aaagatccgc cgagcacttg ctgttcttcc cacaatgctt ccatcattga atactagtgt
    38581 cctaacatca gctggtaaat ctagttcttc agcatgtgag attgttaaat ctgtgatgat
    38641 cgttcctgga ctcccttttg ctagttgata agctgtcttc ctatcaaaaa cttcatgaac
    38701 attattaccg taaaatgtcg tttctacaat cgatcgaata ctggataaca gtggatttcc
    38761 acttgtgatt tcgtttaatt cgagactctc ttttgtactc atggctcctc ctcattccct
    38821 aaacaaaaaa acgttttctt tattatacat tattcgagtt tttatttgta ctgctgcgct
    38881 gtgggaagca aacgagttat ttttcacaca aaaaaaccga gaacttgaat aaacaagttt
    38941 ctcggtttca tcattttcct tttatcggct tagtaccagc cgtttgctaa ccagaatgtt
    39001 ttagcagcat cccatgaacc ataacgagat gttacatatt gttctgctac acgttcttga
    39061 ttggcagctg aatagtcgcc gtttaagtat gaagcatcta attgataacg gccgatgtaa
    39121 cgtccgttag tagctgtgta tgaaccgcct gattcttttt gtgcgatcca ttcttttgca
    39181 gagcttgaag ttggcgctgt tgcagcagct ggggcagcag gagctacttc aactgtttca
    39241 acaggtgcaa cgtatgtatg ttcttgcgtt gctgtttcag ttgtagatgc tgtattttct
    39301 gttgttttgt tttctgttgt tgctgaagaa tcatttgaag ttgggattgt taattgttca
    39361 ccaacataaa tgcggttgat gtttgcgatc gaattttttt cagcaattgc atcgatcatt
    39421 gagttgtctc ctgcaaattt ttgtgagatc ttagataatg aatcacctga ttttactgta
    39481 taaacttcat cagcgtgagc atttgtagtt cctacaaaca ttgcagtagc accagcagca
    39541 aaagttgtac ctaaaacaat tttcttaaat gaattcatat taaactcctt tttttctccg
    39601 ttttttattc tttcgacgct attaacttta tcatt
//
LOCUS       DAZAID010000017        36189 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000017 DAZAID010000000
VERSION     DAZAID010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.017"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..906)
                     /locus_tag="ACG9BE_000813"
     CDS             complement(<1..906)
                     /locus_tag="ACG9BE_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN3031586.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTL"
     gene            1077..1445
                     /locus_tag="ACG9BE_000814"
     CDS             1077..1445
                     /locus_tag="ACG9BE_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFN3031587.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            1615..2046
                     /gene="mraZ"
                     /locus_tag="ACG9BE_000815"
     CDS             1615..2046
                     /gene="mraZ"
                     /locus_tag="ACG9BE_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFN3031588.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            2067..3026
                     /gene="rsmH"
                     /locus_tag="ACG9BE_000816"
     CDS             2067..3026
                     /gene="rsmH"
                     /locus_tag="ACG9BE_000816"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFN3031589.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            3031..3432
                     /gene="ftsL"
                     /locus_tag="ACG9BE_000817"
     CDS             3031..3432
                     /gene="ftsL"
                     /locus_tag="ACG9BE_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFN3031590.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            3433..5625
                     /locus_tag="ACG9BE_000818"
     CDS             3433..5625
                     /locus_tag="ACG9BE_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFN3031591.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            5651..6613
                     /gene="mraY"
                     /locus_tag="ACG9BE_000819"
     CDS             5651..6613
                     /gene="mraY"
                     /locus_tag="ACG9BE_000819"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFN3031592.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            6627..7988
                     /gene="murD"
                     /locus_tag="ACG9BE_000820"
     CDS             6627..7988
                     /gene="murD"
                     /locus_tag="ACG9BE_000820"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFN3031593.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            8014..9102
                     /gene="murG"
                     /locus_tag="ACG9BE_000821"
     CDS             8014..9102
                     /gene="murG"
                     /locus_tag="ACG9BE_000821"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFN3031594.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            9117..10337
                     /locus_tag="ACG9BE_000822"
     CDS             9117..10337
                     /locus_tag="ACG9BE_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFN3031595.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            10493..11818
                     /gene="ftsA"
                     /locus_tag="ACG9BE_000823"
     CDS             10493..11818
                     /gene="ftsA"
                     /locus_tag="ACG9BE_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFN3031596.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            11841..13082
                     /gene="ftsZ"
                     /locus_tag="ACG9BE_000824"
     CDS             11841..13082
                     /gene="ftsZ"
                     /locus_tag="ACG9BE_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFN3031597.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            13099..13776
                     /locus_tag="ACG9BE_000825"
     CDS             13099..13776
                     /locus_tag="ACG9BE_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFN3031598.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            13790..14389
                     /locus_tag="ACG9BE_000826"
     CDS             13790..14389
                     /locus_tag="ACG9BE_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFN3031599.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            14470..14691
                     /locus_tag="ACG9BE_000827"
     CDS             14470..14691
                     /locus_tag="ACG9BE_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFN3031600.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            14740..15522
                     /locus_tag="ACG9BE_000828"
     CDS             14740..15522
                     /locus_tag="ACG9BE_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFN3031601.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            15543..16250
                     /locus_tag="ACG9BE_000829"
     CDS             15543..16250
                     /locus_tag="ACG9BE_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFN3031602.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            16512..19310
                     /gene="ileS"
                     /locus_tag="ACG9BE_000830"
     CDS             16512..19310
                     /gene="ileS"
                     /locus_tag="ACG9BE_000830"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFN3031603.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(19359..20885)
                     /gene="zwf"
                     /locus_tag="ACG9BE_000831"
     CDS             complement(19359..20885)
                     /gene="zwf"
                     /locus_tag="ACG9BE_000831"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFN3031604.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(20900..21547)
                     /locus_tag="ACG9BE_000832"
     CDS             complement(20900..21547)
                     /locus_tag="ACG9BE_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFN3031605.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(21731..22060)
                     /locus_tag="ACG9BE_000833"
     CDS             complement(21731..22060)
                     /locus_tag="ACG9BE_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFN3031606.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            22237..22965
                     /gene="sfsA"
                     /locus_tag="ACG9BE_000834"
     CDS             22237..22965
                     /gene="sfsA"
                     /locus_tag="ACG9BE_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFN3031607.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            22981..23994
                     /locus_tag="ACG9BE_000835"
     CDS             22981..23994
                     /locus_tag="ACG9BE_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3031608.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            23994..25271
                     /locus_tag="ACG9BE_000836"
     CDS             23994..25271
                     /locus_tag="ACG9BE_000836"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN3031609.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            25334..28036
                     /locus_tag="ACG9BE_000837"
     CDS             25334..28036
                     /locus_tag="ACG9BE_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN3031610.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            28188..28505
                     /locus_tag="ACG9BE_000838"
     CDS             28188..28505
                     /locus_tag="ACG9BE_000838"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3031611.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            28535..28855
                     /locus_tag="ACG9BE_000839"
     CDS             28535..28855
                     /locus_tag="ACG9BE_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN3031612.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            28963..30423
                     /locus_tag="ACG9BE_000840"
     CDS             28963..30423
                     /locus_tag="ACG9BE_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN3031613.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            30491..30712
                     /locus_tag="ACG9BE_000841"
     CDS             30491..30712
                     /locus_tag="ACG9BE_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031614.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            30743..30925
                     /locus_tag="ACG9BE_000842"
     CDS             30743..30925
                     /locus_tag="ACG9BE_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFN3031615.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            30925..31338
                     /locus_tag="ACG9BE_000843"
     CDS             30925..31338
                     /locus_tag="ACG9BE_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFN3031616.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            31461..32642
                     /locus_tag="ACG9BE_000844"
     CDS             31461..32642
                     /locus_tag="ACG9BE_000844"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3031617.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(32713..32877)
                     /locus_tag="ACG9BE_000845"
     CDS             complement(32713..32877)
                     /locus_tag="ACG9BE_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031618.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            33031..33104
                     /locus_tag="ACG9BE_000846"
     tRNA            33031..33104
                     /locus_tag="ACG9BE_000846"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33065..33067,aa:Arg,seq:cct)
     gene            complement(33173..34312)
                     /locus_tag="ACG9BE_000847"
     CDS             complement(33173..34312)
                     /locus_tag="ACG9BE_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3031619.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            34484..34618
                     /locus_tag="ACG9BE_000848"
     CDS             34484..34618
                     /locus_tag="ACG9BE_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031620.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(34611..35246)
                     /locus_tag="ACG9BE_000849"
     CDS             complement(34611..35246)
                     /locus_tag="ACG9BE_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031621.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(35359..35994)
                     /locus_tag="ACG9BE_000850"
     CDS             complement(35359..35994)
                     /locus_tag="ACG9BE_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFN3031622.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(36028..>36189)
                     /locus_tag="ACG9BE_000851"
     CDS             complement(36028..>36189)
                     /locus_tag="ACG9BE_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFN3031623.1"
                     /translation="KKYIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFD
                     FKKKELLGL"
BASE COUNT        11949 a         6164 c         7482 g        10594 t
ORIGIN      
        1 taatgttaat agaactagcc atataaaggc atcatccctt tcagcaatag gtaacttaat
       61 gttcattcca tagatactgc cgacgattgt cggtatagtt aaaataatcg ttaaagaagt
      121 taaaattttc ataatattat tcagattatt cgatacgatt gctgaaaaag tctgattcat
      181 ttgttctact agttttaatt gaatgcgaga agtagtcatt gcctgctctg tttccaccaa
      241 tacatcacgt aaatgatttt tcaaagcatg attttcttgg aaaagtttat tttgcgctag
      301 ttctttcaat gcatctaaat tagcagaagt cgcttctctg aaataaacga ggcttttttg
      361 gatatccatg atttgataaa gttgtttgtt ttcagtggat acttgaagtt tggcttccaa
      421 agtatcgcat tgagattgaa gcgtagctaa ataactgttg taagagagtg tgacctgcca
      481 aatgaattga aagaagatat tgagattagg cgaaatatca ctttttggaa aggtctggct
      541 aaagactttc tttaaaaaca gtggttcatg attcgtaatt gtgataagct tctttttggg
      601 cgtcaaaata atagagagcg ggtatgtatc cacttgaaaa taaccgctag gacttgtttt
      661 agcaaaagga tattgtaaca ataataacgc tggctttgtc aactttgatt gattgaattc
      721 ttctgtacgg gaattttctg catcgtccaa tatggaagtg atgtaatctg acggaatttc
      781 aaattgatgt gtcagctgat gaatctcttc ttgattagga cgttctacac aataccagat
      841 cgtttggtca gtttcctttc cggtattacg cagtttctca ttatctaatg taaaatattt
      901 gatcatgatt ttcctcctgt tgcctgtttc ttttattata acggataggg aattggaata
      961 catggaatgt gtttgtaaga tatagaatat aactgcataa atttaaataa aatgaaacag
     1021 ttgtgtaaag tatatacagt atacttgaag caacgataaa aaaggagttt acgttcatgg
     1081 gcacattcac accacttatc ttgttttggt atatttaccc ggtaatcgtt ttgtttggtt
     1141 gccaatttct tgtaaaatta ttttcgttaa atcgtcgttt taaaataaaa gcccctgatt
     1201 tggcgattcc ttttttgtgg ggcggtatgc atgcattgtc tagaaataca gggacaattt
     1261 ctatcttacc gtttttcttt atttctgttt tgctaatggg aatttgtatt gctgttttcc
     1321 aagcatatta ttatgaagaa attatttatc cgcgttattt caagatgact tggcgtttgg
     1381 tttttctgtt gacaatcgtc ttgtacgctg tcttgatcat tgtgaacata ctttcttacc
     1441 tatgaaaaaa atagaaaagc tgtaaaaagt cttttaaaag gctttttgca gcttgatttt
     1501 taaaaaaatg tttatgaata aaaaaagttg ttcccggatt tcccagaaaa ggtggtagaa
     1561 agtgggggat tgtggtagac taattttagt tagtggagga atggggggct ttcaatgttg
     1621 atgggtgaat tccaacataa tattgatgca aaaggacgtt taatcgtgcc ctcaaaactt
     1681 cgtgaagaac ttggcgaaaa gtttgttctg acacgcggat tggatggctg tcttttcggc
     1741 tatccgatga gcgaatggga aaatctggaa gctaaactga acgaaatgcc gttggctaaa
     1801 aaagacgcac gtacttttgt gcgattcttc tattcggctg ccactgaatg cgaacttgat
     1861 aaacaaggac gaatcaatat acccagcacg ttaagaaatt atgcagcttt gacaaaaggt
     1921 tgcgtagtca tcggtgtatc taaccgaatt gaaatttggg atgagacacg atggcaagat
     1981 ttttctgcag cagcagaaga gaactttgac gaaattgctg aaagtatgat tgatttcgga
     2041 ctataagaat aggaagaggc tgactaatga ctgaaacatt tcaacactat acagtcatgt
     2101 tgaaagaaac ggtagatgga ttgaacatca aaccggatgg cgtttatgta gactgtacac
     2161 tcggcggtgc cggacatagt gaatatttat taagccaact aagttctaaa gggcatctgt
     2221 atgcgtttga ccaagatcaa aaagcaatcg atcacgccaa gctgcgttta gcttcttatg
     2281 tcgaacaagg tatggtcaca tttatcaaag cgaacttcag agaattagaa gaacgcttga
     2341 gtgagcatgt agataaggta gacggcattt tgtatgatct aggggtttcc tctcctcagc
     2401 tagatgaagc agaacgtgga tttagttatc atcaagatgc accgttggat atgcgcatgg
     2461 atcagtcagc tcctttatcc gcttatcacg taattaatga atattcctac catgaattag
     2521 taaagatttt cttccgctat ggcgaggaga aattttccaa acaaattgct cgtgaaatcg
     2581 aacgagtacg aaaaacaaaa ccaatccaaa caactggtga gcttgtcgaa atcattaaac
     2641 aagtcatccc agctcccgca agaagaaaag gcgggcatcc tgcaaaaaga atttttcaag
     2701 ctgtgcggat tgcagtaaat gacgaactcg gtgccgaaga ggcctcttta gaacaggcaa
     2761 tccggttatt gaaagtcaat ggaagaatca gcgtaatcac attccattca ttggaagaca
     2821 gaattgtcaa aagtatgttt aaagaatata gtacggtaca agatctacca ccgggacttc
     2881 ctgtcatacc tgacgaattc caaccagaac tgaaagtcat tactcgtaaa ccaatccttc
     2941 cttcagagga agagctaaca gaaaacaatc gatcacgcag tgcaaagtta cgtattgcag
     3001 aaaaaataag aatgaaagag gagtagaatg atggcagaat taaagaaaat gcaagattac
     3061 gaatatgatt tacctgaatt ggtggatcag ccggaacaac aaatgcctga ttcttcttcg
     3121 aatcaagaaa tcattcttcc acaatcaccg gcaaaacgat taaagcagat ttctgctttg
     3181 gaaaaaattg ctgtggcaag cgtggcgatt gctattgttg ctttatgtgt tttaacagtt
     3241 atgctgcgca caaatatcag cggagctgaa aaagaaatca cgtcaataca aacagaaatc
     3301 aatgataaaa agcaagaaaa aacgagttta gaacaagaaa aaaacgaact ttctcgtact
     3361 gaacggatta aacaaatcgc agaagaaaaa ggattgtcta ttaatgacga caatttaagg
     3421 aaagtgaagt aaatgagtct taaaaataaa attcggcggt ttatggaaaa gaaaaatcta
     3481 aatccgatga acaatcgcaa aaaagtaggt atcattttat ttgctacgag tattggattg
     3541 ttctttttat ttgccgtccg tttctcgtat atcgttattg gaggtcatgt tgctggtacc
     3601 tcattagctg aaaaaacaaa acagttgtac caaggaagtg aagtagtaaa agcaaaaaga
     3661 ggaacgattt atgatcgtaa cggtgtggcg ctcgcagaag atgcctcctc ttattcaatt
     3721 aaagcgatac tctcaaaaac atatacctct ggggataaaa aactgtatgt tgaagaaaag
     3781 aattttgata aaatagcaga aatcctgcat aagaatcttt ctatagacaa aaaagatgca
     3841 ctgaatattt tagaggacgg cgcaaaaaaa gagctgtatc aagttgaatt tggtagctat
     3901 gggaaaaata tctcccaaga aaccaagcaa aacatcgaag cggacatgaa aaaagaaggt
     3961 gtcgctggtc tttattttgt cgaccatcag gctcggatgt atccaaatgg cgtcttttct
     4021 tctcatttta ttggatacgc agttccagac aaagatgaga atggcttagt tggtaaacta
     4081 ggtttggagt ctgcttacaa tgatatcttg agcggtaaag atggtaagat tatttatcaa
     4141 aaagataatt ttcaaaatcc tctgccaggt acagtggcag aagaagaaaa agctgtcgac
     4201 ggacaagata tctacacgac tttagacagc agactacaaa gttatttaga aacactgatg
     4261 gatcaagtaa atgaagaata tcagccagaa gaattgacag cagtcctgat gaaagcaaaa
     4321 accggagaaa tcttggcaat gggacaacga cctactttca atcctgaaac aatggaagga
     4381 ttgactggaa aagatgctat ctggcgtaat ttcttggtac aagatagtta tgaaccagga
     4441 tcaaccatga aagttttcac tactgcagct gctatcgaag aaggagaatt caacgagaat
     4501 gaaaccttcc aatctggaaa aatccaagta gaagatgcta cgatcaatga ccatgacttt
     4561 ggtgaaaaag gtgtgctgac catgcgtcaa gctctttcat ggtcaagtaa cgtcgggatg
     4621 gtcatactgg aacagcgatt aggcggacga tggtataact atttacaaaa actgggattt
     4681 ggacaaagta ctcattctgg attagatgat gaagtaaacg gtgctttacc tacattaaat
     4741 attgtcgacc gagcaatgag tgcttatgga caagcggtag gtgtgacgaa tttccaaatg
     4801 atgaaagctt tcacttctat tgcgaataac ggtacaatga ttcaacctcg atatatcagc
     4861 aaagtggtgg atccccaaac tggagaagag cgaacaactc aaacagaagt tctagggcaa
     4921 cctttttcaa aagaaacaac agaaaaagtc cgtgaatata tgagagatgt tgtcgaaagc
     4981 gaaaactacg gtagtgctta tggtgtatat agtgtacctg gatacaatgt atcggcaaaa
     5041 aacggaacag cccaaatcgc ttctgataca gggggatatc aaacaggaga tacagcctac
     5101 ctgtattcga ttgtggagat ggttccttct gaagatccag actatgtctt gtatctaaca
     5161 atgaaacacc ctaagacata tgatcgaatg gcattggcta aaatcgcaaa tccattgatg
     5221 aaacgggcaa tggattttaa agaaactgaa gaagattcag atacagaaac aaaaacagaa
     5281 aaagtatccg tagccgatta tcgtaatctt gaagcggacg ttgcagcagc tgatgctcaa
     5341 aaaagtggac tgcaacctgt agtgatcggt aatggtaaaa aagtacaaaa acaatctact
     5401 gccaacggtg atcagttgat ctctggagaa aaactgatcc tatatactgg tggtgataag
     5461 ctgatgcctg acgtaacagg atggtcaaaa gcagatatca tgaagctggg gaaaatcctc
     5521 ggtattgaag ttagttttga tggagatggt tattgtgtca aacaagaact tgctccatat
     5581 gaaaaaatca ctgaagacaa acttagtttt actttagaag aataagaaga gaaataggag
     5641 agaaaagagc atggaatgga cacaagcatt gattcccatt gtcagcagtt gtgcaatgac
     5701 aattgccgca atgccgctat ttattggtta ttttcaaatg aaaaagcaag gtcaggcgat
     5761 tcgtgaggag ggaccaaagt ggcacaatag caaagccgga actcctacaa tgggcggctt
     5821 agtgtttctg atcggctcta ttttgacagg aatttgggtt ggagcttggc aaaaacagct
     5881 aactcctact cttttcatct tgctttttgt attagcgtta tacggagtaa tcggctttct
     5941 tgatgatttt ataaaaatat tcaagaaaag aaacatggga ctgaactcga agcagaaatt
     6001 gattggacag attctagggg gaatcatctt ttatcttgtt taccgttctg aaggttatcc
     6061 aggtgtattg aatttcttcg gactagatct acctttagga atcatttatg gtattttcgc
     6121 tctcttctgg cttgttggat tttcaaatgc agtaaatctg acagatggca tcgatggatt
     6181 agttgctggc ttaggaagta tctcttttgc agcttacggt ctaattgctt ggcatcagca
     6241 tcagtatgat gtattaatca tttgcttaag tgttttagga ggtttactgg gtttcttcgt
     6301 ctacaacaga aaacctgcca agatttttat gggagatgtc ggttcattag cgttgggcgg
     6361 acttttagcg gcaatttcta taatgctaaa ccaagaatgg acattgcttt tgattggttt
     6421 gatctatgtt atggaaacag caagtgtgat gcttcaagtt acttccttta agcttacagg
     6481 aaaacgaatt tttaaaatgt ctccaattca tcatcacttt gaaatgtgcg gatggtcgga
     6541 gtggaagatt gatacagttt tttggttgat cagcattgtg acttcgttga tcacattatg
     6601 gatcgtttgg taggagatga cgggaaatga aaaaaatatc aacatatgaa aataagaaag
     6661 tgcttgtctt aggactcgca aaaagcggag taagtgcggc caaattatta catgaattgg
     6721 gcgcacttgt caccgttaat gatggaaaac cttttgacga aaatccggaa gcacaagagc
     6781 tgctttcttt agggataaaa gtaataacag gtagtcatcc aatcgaattg ttggatgaag
     6841 aattttcatt gatggtcaaa aatccaggga ttccctacag tcatccttta gtagcaaaag
     6901 cccaagaaat gggtattccg gtaattacgg aagttgagct agcttacgaa gtagcggaat
     6961 gcccaattat tgggattaca ggaacgaacg gaaaaacaac gacaacaacg atgacaggac
     7021 tactactgaa tgcaggagca gatcaaggaa ttgcacgttt agcgggtaat attgggtatc
     7081 cagcaagtgg tgtagcgcaa gaagcaaaat cagaagataa aattgtgatg gaattgtcta
     7141 gcttccaact catgggaatc acagatttcc gccctcatat agcggtgatt accaatatct
     7201 atgaagcaca tattgattat catggtacaa gaaaagaata tgtcaaagct aaatggaacc
     7261 ttcaaaaaaa catgacagaa aaagattatc tgatccttaa ctggaatcag tcagaactac
     7321 aagaattagc gcaaagaaca aaagcacgcg tactgccttt ctcgacaaaa gaagtgttgg
     7381 aagacggtgt gtatgctgac gattactcaa tatactataa aaaagaaaaa ataatggaaa
     7441 tatctgaact tggcgtacca ggtaagcata atgtagaaaa tgcactagct gctatttctg
     7501 tagcaaaatt gtatggtata tcaaatgaag ctatacgaga aacgttgcat tttttccatg
     7561 gcgtaccaca tcgtacgcaa tatgtaggag aaatccaagg cagaaaattt tataatgatt
     7621 caaaagcaac caatatttta gcgactaaaa tggctctaag tggatttgaa acgtctaagg
     7681 tcgtgttatt ggctggcgga ttagacagag gaaatacatt tgatgaatta attccttcat
     7741 taaaaggaat caaagcaatg gtcgtatttg gccaaacaaa agaaaagttg atggatgctg
     7801 gaaaaaaagc aggcatcgag acaatagtaa cagccgattc tgtagaacaa gcagttcctt
     7861 tagcattaga aaacagtaca gatggtgatg tagttttatt atcaccagcg aatgccagct
     7921 gggatcaata cccaaatttt gaaactagag gaaacagatt catggaagct gtgaaccgat
     7981 taaaatagaa aaagtagaag cgagcgataa aaaatgaaaa ttttggttac aggcggagga
     8041 acagggggac atatttatcc agctcttgcc tttgtcaatt atgtaaagac tcaagaaccg
     8101 aatgctgaat tcatgtatgt gggagcaaaa cgaggtctag aaaacaaaat cgtccctgat
     8161 acaggaatgc ctttccatac cttggagatt caaggattta aacgaaaaat ttccatgcat
     8221 aacatcaaga cggtccgatt gtttcttaag agtatccgtc aagcaaaaaa aatcctcagt
     8281 gattttcaac cagatatcgt tatcggtact ggaggatatg tttccggagc ggtagtgtac
     8341 gctgcagcca aaatgggtat tccaacagtt atccatgaac aaaacagtgt accaggtgtt
     8401 accaataaat ttttaagccg atatgtggat cggatcgctc tttcatttaa agatgcggct
     8461 tccttttttc ctgaaaacaa agcagttctt gtaggtaatc ctcgagcaca agaagtagca
     8521 gatactaaaa aatcagatat tttgaaaaca tttgatttgg atccagaaaa gaaaacagtc
     8581 ttgatttttg gcggtagtca aggagcactt aaaatcaatc aagcagtatc agttttttta
     8641 agtgaatata ttccttcttc ttaccaagtt ttgtatgctt caggagatag atattatgaa
     8701 gagatcctaa ataaaataaa tcctgaaata caaaatatta gtgtccagcc ttatatcaaa
     8761 aatatggcag aggtcatggc ttccagtgat ctgctggtgg ggcgtgctgg tgcaacttcg
     8821 atcgcagaat tcacagctct ggggttaccg gcagtgttgg ttcccagccc atatgttacg
     8881 aatgatcatc agactaaaaa tgcaatgagt cttgttcacg caggagcagc aaaaatgatt
     8941 gcagataatg aactaacagg tgaatcacta agtcagacag tcaacgaaat catgggtgat
     9001 gaagaattgc aaaagcaaat gtgccgagct tctaaagaac aaggaatccc tgatgcttca
     9061 aaacgtcttt atgacttagt aaaacaaata atcaaaaaat aaaggcggtg gtcttagtga
     9121 gtaagaaaaa agaaccaaaa aaattgtctc atccgtcttc tgatgaaaac aatttgactc
     9181 cttggcaaaa ggctaaccga aaatatctgg cagaacaaga gatgaaaaac caagagccaa
     9241 aaaaagaaga cataacatta gatacacccc tttcagattc tgaagaagaa aaaacggttc
     9301 aagaggaaca gcaagaagaa agccagtccg agaaaaaaat cgaatttttt gaagagatca
     9361 agcaaaagaa aaacggtgga ccatataacg gttctttttt gaaccggctg cccaatttaa
     9421 aaagtcaacg gcacaaagta ctatttcgtc ggttagcttt gatcatcact gtattgggta
     9481 taccgctgat ttttttgatc tattatgttt ctccttatag caaattacaa gcagttgcag
     9541 tatcaggcaa taagacagtc aacagccaag aaatcatctc tgatacaaaa ttgtcattag
     9601 gagaaaatgt ttggggacag tattttcatc gatctactta tatcgatcgc cttaaaaaag
     9661 cacagccaag aatcgaaaca gcaaatatac atttcaaagg gatgaatgaa ttcgatttgg
     9721 atgtaacaga atataaagag attgctttga tcgcaaaaaa caatcaatac tacccagtga
     9781 ttgaaaatgg aacagtatta gatgaaaaag tagcaaatcc tacaaaaaat ctccctatct
     9841 tggaagagtt caaagactct gcaaaaatca aagaattaac aaagcaatac aatcagttat
     9901 catcagaatt acaaaaagca atttctgaaa tcaagtacac gccaagagca agcaataaaa
     9961 atctgatcca attgaatatg aatgacggca atcaagttat tgtaaatatc aataatcttg
    10021 cgaaccaaat gaaatattat tcgcaagtag ccaaagatat ggatgaaaaa ggtgtgatcg
    10081 acatggaagt tggtatcttc tcgcatcctt acggtagcac tcaagaaacg gaagaatcgg
    10141 gccaatcgga agaaacaact gaaagtgaag aaaatgcgga tcaaacaaac gaatcaagtg
    10201 aagaggcaga aacgcaaaaa gcacaaacac ctggatcgtc tagcacaaac ggatcatctg
    10261 attcaaatga ttcaagtgaa acaagtcaaa tagaagataa tccaagtaca actccgtcaa
    10321 gtttaaatga aaattgataa aacaacttta aaaagtaaga atgtactttc taaaaaccag
    10381 taatttatgg tagaattgga agcagtgaat atatctatta ataagtatat tttaaaacgg
    10441 acgctagttg ttcgcttgta tatggaatag taagtaggag ggaatcccat tcatggcaaa
    10501 aacaggaatg tatgtaggcc ttgatattgg aacgacatct gttaaagttg tcgtggctga
    10561 atatatcgac agccaaatga atattattgg agtaggaaac gcaaaatcag aagggattaa
    10621 ccgaggcatt atcgttgata tcgataaaac ggtccaagcg atacaacgag cagtacgaca
    10681 agcagaagaa aaagcaggta tccagatcaa aggcgtaagt gtcggtttgc cagcaaacat
    10741 gcttgaagta gaaaattgcc agggaatgat tgcagttaac ggagattcaa aggaaatcac
    10801 cgatgaagat gtcagaaatg tggcttcagc tgcccttgtt cgttcaatac ctcctgaacg
    10861 tcaaatcgtg tcaatattgc cacaagattt tactgtcgat gggttcgagg gaatcaaaga
    10921 tcctcgtgga atgatcggcg taagattaga aatgtacggt ctgttgttta caggacctaa
    10981 aacgatcatc cataacatac gtaaatgcgt ggaaaatgcc ggattaatcg tcaacgaaat
    11041 ggtgattact cctttagcgc tgactgaatc aattctttct gacggagaaa aagattttgg
    11101 tacgatcgtc attgatatgg gtggcggcca aacaacaacg gctgttatgc atgataagca
    11161 attgaagttt accaatcttg accaagaagg tggcgaattt gtcactaaag atatctctat
    11221 tgtattaaat acgtcattta acaatgcaga agcgttgaaa ataaactacg gggatgcata
    11281 cccagaacgg acatcaccag atgaagaatt tccagtagat gtgattggtc agtcagaacc
    11341 agtgaaagtc gatgaacgct acttgtcaga aattatttca gctcgaatgg aacagatttt
    11401 caacaaagca aaagatgcat tggaccaaat cgaagcatta gagttgccag gtggcgtcgt
    11461 attgacaggc ggagctgcta gtcttcctgg tgttgtggac ttggcacaag aaatcttcgg
    11521 cgtaaatgta aaattgtatg ttccaaatca catggggctg agaaacccgg tatttacaaa
    11581 tgtaataagt atcgtggatt attcagcaaa cttgagtgaa gtgtatcaat tggcaaaaat
    11641 tgctgttacg ggtgaaacct cagcagctcg tcaaatggta gttgaacaag aaacaacaaa
    11701 cacatatgag agctatgaag cacctgaaga aacaatctac gatgagccgg aaccaaaaga
    11761 gtccggagaa aatgtaaaaa ataaaatcaa gggtttcttt acaaatattt ttgattaatt
    11821 catcctggga ggaataagat atggaattct caattgataa taatatcaat gacggcgcag
    11881 taatcaaagt catcggagtc ggtggtggcg gtggaaatgc tgtaaaccga atgattgaag
    11941 aaaacgttaa aggtgttgaa ttcattacag ccaacacaga cgtgcaagca ttaaaaaatt
    12001 caaaagctga aacagttatc caattaggcc ccaaatatac tcgtggttta ggtgctggtt
    12061 ctcagccaga agttggacaa aaagcggcag aagaaagtga acaatcctta cgcgaagctt
    12121 tagacggtgc agatatgatt ttcatcactg caggaatggg tggaggaacc ggtacaggtg
    12181 ctgcaccaat cgttgcaggt atcgcgaaag aattgggcgc attgactgtt ggtgtcgtga
    12241 cacgtccatt cacttttgaa ggaccgaaac gtggccgttt tgctgcagaa ggtatcgctc
    12301 gtttgaaaga aaatgtggat acattattaa ttatctcaaa taaccgttta ttagaagttg
    12361 tagataaaaa gacaccgatg cttgaagctt tccgtgaagc agataatgtt ttacgacaag
    12421 gtgtacaagg tatctctgat ttgatcacag cgccaggtta cgttaacctt gactttgctg
    12481 acgtgaaaac agtgatggaa aaccaaggta cagcattgat ggggatcggt gtggctagtg
    12541 gagaagatcg cgtagtagaa gcaacgaaaa aagcaatctc ctctccattg cttgaaacat
    12601 ctattgatgg agctgaacaa gtacttctta acatcacagg cggtttggat atgacattgt
    12661 tcgaagcaca agatgcttca gatatcgtag caaatgcagc aactggcgat gtaaatatca
    12721 ttctaggtac atctatcaat gaagaaatgg gcgatgaaat ccgtgtgacg gtcatcgcaa
    12781 caggtatcga tgaatcaaaa aaggaacgca agtctagccg accagctaga caagcacaaa
    12841 tgcaatcacc tgctcaaaag actgtattag atatggatca agctaaacca acttcttcag
    12901 aagaagaaaa cagttttggc gattgggata tcagacgtga acaaaatgtt cgtcctagag
    12961 ttgatgattc caattttgac aatatcgaaa aaaaagaatt tgatacattc aaccgcgaag
    13021 aaacaaaatc tcatggcgat gatgaattaa atacgccacc gtttttccgt cgtaaaaaat
    13081 aaaggagagg aagagcacat gattgctgac aacttgcaga aaatcaagca agaaattcag
    13141 gattcgtgtg ctcttgttca tcgtgatccg aaagaagtta cattagtagc tgtaacaaaa
    13201 tctgttgaat ccgctcaagc tgaagaattg gttagagctg gcgttatgaa ttgtgcagaa
    13261 aaccgagtag ataaattatt agaaaagaaa caagatttat ttgattttcc tgaaataaag
    13321 tggcatttaa ttggtaattt acagcgcaga aaggtaaaat tgattgtaaa tgaaattgat
    13381 tactttcatg cgttggacag tttaagatta gcagaagaaa tccagaagcg gttagagaaa
    13441 gaattatcct gttttattga agtaaatgtt tctggtgagg aatcaaaaca tggaatttca
    13501 ccagatgagc ttttaccgtt tgtggaatct ttagctgcct gcacaaaaat caaggttgtt
    13561 gggttgatga ctatggcgcc taaagaagct tctgatgagc aaatccataa agtgtttggt
    13621 acgctgaaag aattgcgtga tcttgttcaa gcaaaaggtt atgcccatgc accatgtagt
    13681 gaattaagta tggggatgag tcaagacttt caaattgcga ttgaagaagg tgcaacattt
    13741 atccgtgtag gaacagcgct attcaaagac gaataaagga ggaacaaaaa tgccgctttt
    13801 aaacagagga gcaattgcta attttttcgg attagctgat gaggaagagt acgaatacca
    13861 agattatcct gaaacaacaa aaaaggctgt acagccaact aaaaaaaata caacacagaa
    13921 tcaatcaact cattctgtat ctgctgcgaa tactcatgaa aaacaaaaaa cggcgacttc
    13981 acgtcctgta acgcatgaaa gtattcagcc ggctgaaaag aaaaaagtaa ttgaactaca
    14041 tcaatcacct acaaaaactg tagcgaaaga aagaacagct tcaagaacct ctacacaacc
    14101 taaagcaaat cttgctggaa aaatcactgt aatggaacca agagcttatt ctgaagctat
    14161 ggcgattgct aaacgaatca ttgctggaga agcaacctta gtgaattttc atttagtaga
    14221 agaaaaacaa gcgagaagaa tcgttgattt tctaacagga acagtttatg cactagatgg
    14281 agatatcaaa cgtgttggag atgagatttt tctttgtaca ccgactggga tggagattga
    14341 cagttcaacc gctcaatctt ttgcagatgc gaatttgttt gatctgtaag aggaggaact
    14401 acgatttata ctattgcact attacttcat ttacttcaac gggcagccta tttctatagc
    14461 attatcttga tggtatatgc attgctctct tggtttccag gcgggtatca atcagggcta
    14521 ggacgattat tagctaagat ctgcgaaccg tatatcagtt tgttcgatcg tctgcctttg
    14581 aggattggac ccttggattt cacaatttta ggggcaatcc tgatcttgaa cttagccgta
    14641 caaggtttag cacagatcgt tatatggata actgtccatt tagtttattg attgaagcaa
    14701 caaaatcttt attctcagaa tagataggag gggaaaattt tggatgcaaa tgtataccag
    14761 cactttcggg cagatgaacg tacatttatc gattcggtag gcgattggat tgaacaggtt
    14821 caaggacaat atgcacccta tctgaccgaa tttcttgacc caagacaatc ctatatttta
    14881 gaaacactga ttcgacaaag cagtgatttg cgtttcatgt tttatggcgg atatgaacag
    14941 gcagaaagaa aaagatgttt gattttcccc gattattacg aacctgtaga ggaagatttt
    15001 gaaacagcat tgtatgaaat ccattatcct agcaaatttg ccagcctaag tcatgggaaa
    15061 atccttggaa ctttagtggg aacggggatc aaacgctctt actttggcga tatcatctcg
    15121 gacggtgatc gctggcaagt atttattgca aatgaggtgg atcattttgt tgctgcacaa
    15181 gtaacaaaga ttggaaaggt tgctgtacgc ttggaaccaa tcaaatacac agaaatgatt
    15241 atgccgaaag atagctggac acaagaaaga ggaacagtta gttccatgcg actagacagt
    15301 gtgatctctg agatttataa tatttccaga cagcggtcca agcagctgat cgaagctggt
    15361 aaagtaaaag tgaactggac agaaaataca agacctgatt ttgtacttga actattagat
    15421 attatttcag tcagaggtta tggcagagtc cagatccaag aacttgaggg gaagacgaaa
    15481 aaagaaaagt acagggtact gttcggtgta ttacgtaaat aaaataaaaa ggtggtttac
    15541 tcatggcttt aactccatta gatatccaaa ataagacatt cccgacaaaa atgcggggat
    15601 ataatcaaga cgaagtagat gatttcttag atttagttgt tcgtgactat gaggaattga
    15661 ctcaaagaaa tcgagaatta gaaaaagcag tcaaacattc tgaagaaaaa cttgagtact
    15721 tcaatgaatt aaaagatgct ttgaaccaat caatcatcgt agctcaagat acggctgata
    15781 aagtaaaaac aagtgcaagc aaggaatccg aagtgatcgt tacttctgca caaaacaaag
    15841 cagatgagtt agttgcgaac gctgaaaaac gtgcacatca attaacaact gatgcagaag
    15901 aaaaagcaag aaaaatcttg acagatgcaa cagaaaaagc aagacagctt gcgacagaaa
    15961 ctgaagatct taagaaaaaa acacgagtat tccatcaacg tatcagcttg atgcttgagt
    16021 ctcaattaga acaagtgaaa agtccggaat gggatgaaat tttacaacca ttctccagct
    16081 acgtgactga cagccatgaa gtgatcaaag aagttttagc aaaacagctt gacaatgaaa
    16141 atgatactga tgtaaactca gacacaggag tcatagaagc acctatgacg gcatttgata
    16201 cacaggctat tgatatctcg atcgtgcctg gtgaaaatga aaacgagtag aacagaaaag
    16261 cactttgtta ttcttaaagc gagccgatga gagtggaagt tcggtaagac actgcatggt
    16321 gaaagatcct ctacttgaga cctgcttttc cgaaattaga gtaagaagca gcggctgacc
    16381 tcgttattgg tcacagagag tttgcactag atagatgcaa ctaaatttgg gtggtaccac
    16441 ggcatgttcg tcccttgcgg ctgaacatgc ttttttgtgt ccggaaaaca aataaaggag
    16501 tgtatggttg aatgaaaatg aaagaaacct tgcaattagg aaaaacaaaa ttccctatgc
    16561 gtggtaattt gccaaaccga gaaggcgagt ggcaaaaaga atgggaagaa aatcgaatct
    16621 accaaaaaag acaagagttg aacgaaggaa aacctagctt tgttcttcat gatggacctc
    16681 catatgccaa tggtaatatc catttaggac attcattgaa taaaatcagt aaagacatta
    16741 tcattcgttc aaaatcaatg tctggctatc gtgcaccata tgttccagga tgggatacac
    16801 atggattgcc gattgaacaa gtattggcta ataaaggcgt gaaacgtaaa gaaatgtcca
    16861 tggcagaata cttggaaaaa tgtcgtgaat atgcactttc ccaagtagat aagcaacgag
    16921 cagatttcaa acgcctagga gttgcaggag attgggaaca tccttatgtt acattagatc
    16981 cttcttatga agctgcacaa gtacgcgtat ttggtaaaat ggctgaaaaa ggctatattt
    17041 acaaaggatt gaaaccaatt tactggtctc cgtcaagtga gtcttcttta gcggaagctg
    17101 aaattgaata taaagatgta aaatctccat ctatttttgt agctttcaat gtcaaagacg
    17161 gcaaagatat tcttgatgaa gatacagctt ttgtcatttg gacgacaaca ccatggacat
    17221 tgccagcaaa ccaaggtatt gctgtaaatc caaaatttac gtatgtattg gtggaagccg
    17281 atggccgtaa gtttgtcgta gcgaaagatt tgctagaaac agtccaatca gaaatcggtt
    17341 gggaaaatgt aacagttcta aaagaatttg ctggacaaga aatggaatat atgactgcaa
    17401 gtcatccatt ctatgaacgt gaatcacttg tgattttagg cgatcatgtt actttagacg
    17461 ctggtactgg attagtccat actgctccag gacatgggga agatgactat atcgctggta
    17521 gcaaatataa actatctgta gtgtcacctg tagactcaaa aggattgttt acagaagaag
    17581 cacctggatt tgaaggtatc ttttatgaca aagcaaatcc tatgatcact gatctattaa
    17641 aagaaaaagg agctttgctg aagctagatt tctttactca tagttatcct cacgattggc
    17701 ggacgaaaaa gcctgtgatc tatcgggcaa ctccacaatg gtttgcatct atcgataaat
    17761 tccgtcagaa tattttggat gaagtagaaa aagtagagtg ggttatccca tggggtaaaa
    17821 cacgtttgta taatatgatc cgtgaccgcg gcgattgggt aatttcccgt cagcgtgcat
    17881 ggggagtacc acttccgatt ttctatgctg aaaatggaga agcaatcatc acaccagaaa
    17941 caatcgaaca tgtggcaaaa ttatttgctg aacatggatc aattatctgg tttgaaaaag
    18001 aagcgaaaga acttcttcct gaaggattta cccacccagg ttctcctaat ggcgaattta
    18061 caaaagaaaa agatatcatg gatgtctggt ttgattctgg ttcttctcat gaagctgttt
    18121 tacgtcagcg accagaatta tcattcccag cagatatgta tttagaagga tcagaccaat
    18181 atcgtggctg gttcaactca agtatcacga ctagtgtggc tatcaatggt gtagcaccat
    18241 acaaatcagt tatttctcaa ggatttgctt tagatggtga aggccgtaag atgagtaaat
    18301 ctttaggaaa catcattgca ccagaaaaag tgatcaaaca atttggtgca gatatccttc
    18361 gcttatgggt atctagtgta gattatgaag cagatgttcg tgtttctatg gatatcttaa
    18421 gtcaagtggc agaagtatat cgtaaaatca gaaatacgat gcgtttctta ttagcgaata
    18481 cagaagattt cgatcctaaa aaagatactg tttcgtacaa tgatttacgt tctgtagaca
    18541 aatatatgac cgttcgattg aaccaagtaa tcaaagaaat ccgagaagaa gggtacgaca
    18601 aatataactt catgcatatt taccgtacag ttatgaactt cttgacggtt gatctatcgt
    18661 cattctacct ggattttgct aaagatgtag tttatattga agcagaagat gattatcaac
    18721 gtcgttgcat gcaaactgtt ttctatcaaa cagctgtagc tttgacaaaa ttattgactc
    18781 ctatcattcc gcatacagca gaagaaatct ggtcattcct gaaagaagaa gaggagtacg
    18841 ttcagttaag cgagtttcct ggttatgaag agtttgcaaa ccaagaagaa ttgatggata
    18901 cctggactgc tttcatggac ttccgtgata atgtcttaaa agcattggaa gaagcaagaa
    18961 acagcaaatt gattggtaaa tctttggaag caaaactaac tgtttatcca aaacaacaag
    19021 ttcgtgaaat gttaaaagct ttggatgcag atattgcaca attattgatc gtttcaccag
    19081 actactttga agttatggca gcagatgaac aagtgccaga taacgctatg gtatttgatg
    19141 atgttgctat tacagtggaa aaagcagacg gggaaacttg cgatcgctgt cgccaagttc
    19201 gtaaagatgt aggagtagat gaaaaacttc ctcatctatg cggtcgttgt gcaaaaatcg
    19261 ttgaagactt ctatcctgaa gctgtagctg aaggattcga agaaaagtaa aagggaaaag
    19321 ggggtgtgac atgttctttg tcacaccccc ttttgttatc aatcgtttaa ttttccacgt
    19381 tcacgatacc agttatctgg ttgccagatc cattcgaatc cgtctttttc aagtagttcg
    19441 aaagctgctt taggtcccat tgatccagct ttgtagttgg gaaaatccgg ttgtgtttgg
    19501 tcccaagctt ttcgaatgac atccacgata tgccaagatt gagcgacttc ttcccaatga
    19561 gagaagttcg ttccatcacc attcaacgca tctaatagta atttttcgta agcttctggg
    19621 ctattttcaa ccatttctgc actatttcta aattccaatt tgattggttc cgtttcaaat
    19681 ccttgcccta cttctttccc attcaaggat aaagaaaatc cttcagttgg ctgaatatag
    19741 atcgttagaa cattaggtgg aagtgtcgta tcgtcacatg gttcatcgac agatgttttg
    19801 aatacattaa caggaacttg tttgaatacg atgttgatcc gagtcccttt ttcagtcaaa
    19861 cgtttgcctg tacgtacata gaaaggaaca cctgaccaac ggaagttatc gatagtgaat
    19921 ttgcctgcga cgaatgtttc agtctctgat tgagcatcca ctttgtcttc ttcgcgatag
    19981 cccttgaatt gtacaccatc caattcacct tgagcatatt gcccgcgaac gaagttttct
    20041 aacacttctt cttcgttata aatgcggata gctttcaatg ctttgatctt ttctgtacgg
    20101 atttcttttt cggaaaatgc tactggcggt tccattgcaa gtaatgctac aacctgtaaa
    20161 atatgatttt gcaccatgtc tttaagtgcg ccactatgat cgtagtagcc cccgcgatct
    20221 tctacaccga ggttttcgcc aaaagtaatt tggatattgt cgatatagcg attgttccat
    20281 tgagcctcaa aaatattatt ggcaaaacgg atagctgaga tgttttggat catttctttt
    20341 cctaaataat gatcaatcct gaaaatatct tgttctggga atacttctct gatttgctgg
    20401 ttcaattcaa aagcagactg gtaatcagaa ccgaacggtt tttctatgat caaacgattg
    20461 aagccatttt ttgtcataat attttgtgat ttcaaatgag aaacgatcgt tccaaaaaat
    20521 tgaggtgcca tagccaaata atagacacgg ttaccgttta attgatagtg tccatctaat
    20581 cggtcagaca attcttttaa tgtctgataa tgcgttgaat catttacatc atgggattga
    20641 tagtaaaaat ggcttgcaaa ggtagttgct tcttcatctg aaggattcaa tgcacggatt
    20701 gtttctttga caacttcacg gtagtgctca tcgctccagg gacggcgagc ggttccgatt
    20761 acagcaaaat gctcgcctaa atttcctttt ctgtaaaggc ggaacaaaga cgggtacaat
    20821 ttacgctggg ctaggtcacc tgtaccgccg aatatcgtaa acaatacatt tttttcatta
    20881 gacatgtgga tacactcctt tatattttat cgacaaaaat cgtgctggcc gccttaaagc
    20941 tgacggggat tttctttcta tcattttgga tagtgatcgg tccttcataa gctgcgatgt
    21001 caacgatctc atattcttcg ttgatattca ggtcaatagt gactaagtaa tccaacaatt
    21061 ctttttcatc aagcactcgt gcgatacgca cttttgtacc gacaggatag gactccaatg
    21121 tttgtcggcg ttcttcatgg acaacttcct cttttttagg aatcgatcct ccatgaggac
    21181 aatgttcagg atgattcaaa tagttttcta aatgatctgc aagatgttcc gaagtcacat
    21241 gttccaatac ttcagcatca tcatggactt cattccagct atagtctaaa tgttcaacta
    21301 aaaaaacttc ccataagcga tgtttgcgaa tcagactgct agctttttgc agtccgcttg
    21361 cagtcaattg tacaccttga taaggggaat gttctacaag ctcttctttc acaagtttag
    21421 aaatcatttc actgacagat gcagctgaga catctagact agatacgatt tgtttattgt
    21481 tgactttcgt tgtatctcca cctagttcta aaatgatttt caaataatct tctcgatttg
    21541 gtgtcatatt cgatcatcct ttcatggtaa aaaaccatca ttagtaagtg taacaaaaaa
    21601 aacgttaatt gactattttt ctaaataaaa aacaaaggat ttttagacgt ttttacaaca
    21661 aaaaacgaaa gatgatagag aagactcatt ctttcgttta taaaggatat atatatacat
    21721 tgggagggat ctatttttca gataacaact tatatgaagt gtctgcagat tcgtcataaa
    21781 gagtttgttc gggataagaa aagataagat aaaaaccata ctgatcacgt acgactaaac
    21841 gatattcatc tatttctacc acagatgtgc caagagactg accgttgatc aaaaacgtgt
    21901 aatcagatgt aatctgtaat tgttgttttt gctggctatc actaaagaac catgtacctt
    21961 gatactttga gagtccttct gacttttccg cttgtttttt ctttttcaag taaataaaac
    22021 ttgagagaac ggtaatgagt gaaccaagaa aatagatcat tttttttatc accaaatcac
    22081 tcctttatga gaaaataatc gcatagacat attctttctt ctttatttca ttgtaccgaa
    22141 taaaagctaa aatttctcta agactttcga ctggtttttt tactgttttt ctacaaatta
    22201 tgataggata catagtagga agaaaggtga cataatttga agtatcagca agtggaaata
    22261 gcttttttta ttgaccgccc gaatcggttt atagcacatt gtatgaaaaa agacggagaa
    22321 ataattattg cgcatgtcaa aaatacaggt agaggaaaag aggtcctgat accaggcgct
    22381 gaagtggcta ttgtatttgc tcctggacct aaacggaaaa ccgcctatga tctgattgct
    22441 gtaaaaaaga agggtgactg gtttaatatc gatagtcaat tgcctaatcg gctggcaatt
    22501 gatggtattt tagacggcac gatcctttta ccaggattaa ctggtccatt tacggtatac
    22561 aagcgtgaag taacttatct tcactccaaa tttgatattt atttagagac ggtggtcgga
    22621 caaaaagcat ttgttgaagt caaaggtatg acacttgaaa ataaagcgat tggtgctttt
    22681 cctgatgcgc ccacactcag aggattgaaa catataggag aattaacgta tgccgcacaa
    22741 gatggttatg ccgcttatgt cttgtttatt gctcagttcg aacatcttca tctggcaacg
    22801 atccatgaag aaatgcaacc agcactagct gatatggttc gacatgcgca gcaaagcgga
    22861 gttcaaatcc tcgcctataa ttgtcaagta tcggcagatg aggtaactgt caagcaggcc
    22921 attccatttg atgtagatat cccttttgaa gatcctatac aatagaaaga aggtaaaata
    22981 atgatcaact taggtattat cggaacaaat tggattactc atcaatttgt ccaagcagct
    23041 ttaagcacaa aaaaatatga attgacagcg gtatattcta gacatttgga aaaagcacag
    23101 gaatttggtt ctcattatga aggggatatc gaattcgcaa cagatttgaa gacttttttc
    23161 aatattactc atatggacac catttatatt gcttctccga atagtcttca ctttagtcaa
    23221 gctaagcaag caattttggc aggaaaaaat gtgatagttg aaaagccagc tttttcgacg
    23281 ccaaaagaaa tggatgaaat catccatttg gcaaatgaac acaatgtgta tttttttgaa
    23341 gcagcaagaa atatccatga atcaggtttt aaaaagattg ccgattactt accattaaaa
    23401 gacgagataa tcggtgcaaa tttcagctat atgaaatatt cttcgcgcta tgaccaagta
    23461 ttagatggag aagaaccgaa tatcttctcg actcatttct caggtggtgc attgatggat
    23521 ttaggtgttt accttgtata tgcagctgtt gcttggtttg gaatgccaaa agaagtccat
    23581 tatttcccaa gaaagatttc tactggtgta gatggtcttg gatgggggat cttacgttat
    23641 gagaattttg atgtatcgat ccagccaggt aaaatcggtg attcttattt accgtcagag
    23701 atttattttg actcaggaac gctagtgatg aatggagtca atgcaattga acaagcacaa
    23761 tttcatgatc gcgcgcataa agaaatcatt gatttggcgg taacagctgc agaaaatcca
    23821 atggcagatg aagcagagga ttttgccaat gtcatggctc atccgaaaga tccagcttgg
    23881 ggattgttgt atgaagagtg gatcgagcta gctagaaatg tgaaccaagt gatttatgat
    23941 ttaagaaaaa atggcggcat caaattcgat gcagataatg aggaagattt ttaatgaacg
    24001 aatttgtaca agtatttccc gaaaattggc agaaaaaata tgaggataaa gggtttatct
    24061 caccttctct tattcaacga gcagtttttc agccactaag taataaacaa agtatagtag
    24121 cggtttctcc tacaggttca ggtaaaacac tggcttatct ttggcctcta ctactaaacg
    24181 tggaaccagg tgaagcaagt gcgttggtca tttttgcttc ttctcaagaa ctagctattc
    24241 aagtagcaga tgttgcacgt gaatggggaa aagacaaaga gttgaaagtc caatctttgg
    24301 tgggcggagc caacgtcaaa cgtcagattg agggtttgaa ggaaaaacca gaaatcttga
    24361 ttggtacacc aggaagaata ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag
    24421 tgaaaacaat ggtatttgat gaagcagacc agctatttga cgctgggaac agccaaatca
    24481 ttgatcaaat tcttcatcaa gctccaacag agtatcagct agcttttttt tcagcgacag
    24541 cagatcgttc ccttgaaagc attgaaaaaa tcactgggaa aacgtttgag acaattgacg
    24601 tgacagcaga ggacgattct cgaaaaggac tgagacatta ctttttacgt gtaccgatac
    24661 gaaagaaaga tgactacttg agacgtctca cgcatataga agattttcat ggtctgattt
    24721 tctttaatca actgactgaa ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac
    24781 cggtaggaag tctggcctct gatcagaaca agctactccg aaaagcagcg ctagaacaat
    24841 tcaaaaagaa aatggtcaga gcacttttga ccacagatgt ggcggctaga ggtttagata
    24901 ttacaggtct tccttatgta gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc
    24961 atcgttcggg aagaactggt cgaatgggta atgaaggaat agtaattaca ctcgtacaag
    25021 ataataatct aagagattta aaacggcttt tacgtccatc aaatataaca cttgaagaga
    25081 tttttttata cggaggtaag ctgcaaactg agcagccaac caaagaagat gcagctcctg
    25141 cagtaaataa aaagtatgcg aaaacttttt ctggaaagga gcagaaccca gaatataaca
    25201 aagaaaaaat aagaaaaaaa gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa
    25261 ggcgcaaata agcgcctttt tatgctatga ttatgaaaac gggaacaaaa ttggttgcca
    25321 gaggaggggt tatgtgaaag aacgtcaaga agagatcttg cagctattga cagaaaacca
    25381 acaaggattg acagcaagtg atgtagctga gcgcttaact atcgatcgaa gcaatgcaag
    25441 ccgatattta agtgaactat acaaggccca tcatattgta aaaaccgctg gccgaccagt
    25501 cgtttactct ttgccaacgg aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac
    25561 gcaagtgact tttgaaacac tcgttggaga aaatgattct ttaaaagtca gcatacagca
    25621 ggcaaaagct gcgattcttt atccaccgcg ggggttgcat acgattattt ttggagaaac
    25681 tggtacaggg aagtcgatgt ttgctgaatg tatgtatcat tttgcaatcg attcagagat
    25741 gctttctgca gatgcgccat ttgtttcttt caattgtgcg gattatgctc aaaatccgca
    25801 actgctcttt ggacacatct ttgggatcaa aaaaggggct tacacaggag cagctcaaga
    25861 tagtcccggt ctaatagcca aagcagacgg cggtatattg tttttagatg agatccaccg
    25921 tttaccgccg gaagggcagg aaatgctgtt tactttcatt gataaaggta tttaccgtcc
    25981 gcttggagaa agtgcacaag tacatgaagc atctgttcag atcattgggg cgacgactga
    26041 atcttcagaa agtttcttat ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc
    26101 gaatcttgct tcacgttcac tggatgagcg ttatgaaatc atctctcttt ttatcaaaca
    26161 agaagcgaat cgtttgaatc aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat
    26221 gctttatgat gcggaaggaa atatcggtca agtgaaacga gacttgaaac tagtctgtgc
    26281 aaaatctttt ttacattacc gtactcatca tgagaaaaag ctaatcattc gaaaagaaga
    26341 actttccctt caagtacaaa aaggattact gaaaatcaag gaaataccgg aacgtctcga
    26401 ccgattcatg gacagcaaaa gtcagtattt gacatttgaa ccaggatttg ctgatgttgt
    26461 ctggtcacaa gatccagaac gaaacatgca ggtctataac gatattgagg aaaaagtcac
    26521 ttctctttcc gaaaccggtg tggagaacat tgatctagag accctcattt caaaagatgt
    26581 ggatgcttat ttccagacat atgtcaaaga attgactaaa agttcgattc ccaaagatct
    26641 tttgccagat gaaatctggc ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt
    26701 agaccgtatt tataatgaaa aagctcgttt tgcttttgct ttgcatctgc aaagtacatt
    26761 ggatcgtgtg agagaaggtc atatgatcgt ccacccagat ttgaataacg tacggaaaaa
    26821 ttttaagagg gaattccagg tagcaatcga tctttcaacg atcattgaag aagaagaaca
    26881 agttgagatt ccttttgatg aaatcggctt tattagtatg tttctttcta tcaatctagg
    26941 agaaagtgag cctgttaaag aaaacaaagt agatgttctc atattgatgc acggcagaca
    27001 gactgcttca agcatgttgg agatggcaca agaactatta ggaacaaaag tagggaaagc
    27061 ttttaatatg ccactggtta tggaagttca aacaatgtat gaagaagtgt tacaatatgt
    27121 caaaaactat caagagcatt tgacgaatgg cctactcttg ttaacagata tgggatcatt
    27181 aaacaccttc agtgatttga tttatgaaga gactggcatt cggacgaaag ctatcacgat
    27241 gacaagtacc atgattgtga tagaagcgat acggatggca agtgttggaa gaagcttaga
    27301 agatatttat caaaatatcc agctttcctt tgaatcgatc gtacgtgatc agttcaaagc
    27361 ggaaaagtct gaaactttac agcgtaaaaa agccgtaatc gtgacatgct ttactggtga
    27421 aggagtagct gccaaattat atcagcgtat tatgccagtg attaatcaaa ataaggtaga
    27481 actgatccag atgcagtttt tagaacgtga atcattcaaa aaacacatcg atgccttatt
    27541 ggaagaatat gagatcaaag caattgcggg aacagttgat gttgattatc aaaatatccc
    27601 atttttttca gcctatgacg tttttgatga tgaaaaacta aatattttaa agcgtatagc
    27661 tgatgatgag atcccgacag ctaaaattgt cgattcactt gctggatcat taagcgatgt
    27721 cttttctatt gatgaactga tccgttcatt gcaaaagaca gttcagcaga tccagaccga
    27781 tttgtcgatt atcgtggaac ctggagtaga tactggaatc gttatccatt tagccttcat
    27841 gttagacgga ttgatcaaac aagaaagcca tcggcaattt gagaaactgt ctattttctc
    27901 aaaaatttat cgtttggaaa tggatatcgt tcgggcaaac ttgatggcac tagaacgaaa
    27961 atatcaggta aaaattccag aagatgatat cgcatttctt acacaaatgt ttcttgaaaa
    28021 taaaataaaa aaatgaacac actaatcata ctgtttacac actaaacatc tgtgtgcgca
    28081 ctataaaaaa gagctaaaaa gcatgaaaac gctatttcag ctcttttttt tattggcatg
    28141 cttcttgcgt tatataaaag tgtaaaaaaa taaaaggagt gttaaaaatg gcaaaaaaaa
    28201 caatcatgtt agtttgttca gcagggatga gtacgagttt attggtcaca aaaatgcaaa
    28261 aagcagcaga agaaaaaggg atggaagccg atatttttgc ggtctctgcc tcagatgcag
    28321 ataataattt agaatctaag aatgtcgatg tattattatt aggtcctcaa gtacgtttca
    28381 tgaaagctca atttgaacaa aaacttgcgc ctaaaggtat tccattagat gtcatcaata
    28441 tgcaagacta tggcatgatg aacggagaaa aagttcttgc acaagcagaa aatctaatga
    28501 aataaatcaa gtatgcagtt gggggtataa caacgtggaa gatcaacaaa atctagaagc
    28561 catcatgggg ttaatcatgt ttggcggtaa tgcaaagagc gatgcaatgg aagcgattgc
    28621 ggctgctaaa atcggtgatt ttgagttagc ggataggaaa atcgctgatg cagaagaatc
    28681 acttatacaa gcgcatcatg cacaaacaga catgttgaca caagaagcac aaggaaatca
    28741 tatgcaagtg acattattga ctgttcacag tcaagatcac ttaatgacat ctattgcgtt
    28801 tactgattta gcaaaagaaa tcatcgaact ttatcgtcgc gtatacagcg aataaataca
    28861 acttcttttt tgaacggaag aagaaaaatt tttgaacgga ttttttattt tgttataagg
    28921 agaactctct ttatataaaa aacttatagg aggaagtaaa aaatgaacgg actatcaagt
    28981 tggatggaga aatacatcct gccggttgcc gcaaaaatcg gagcgcaaaa acatttagtt
    29041 gctttgcgtg atgcttttat cggcatactg cccgcaacaa tggcgggatc agtcgctgta
    29101 atgatcaatg caattattcg tgatttaccg caacagttta acagtaccta tgctgacagt
    29161 ttagcagcag acaaaggaat ctttgccgta ttgaatgtca tcatcggaat caatggtttt
    29221 gtctggaacg gtacactagc aatcgccgga ttgatctttg ccttttcttg ggggtataat
    29281 cttgcccgag cttatggagt aaatgatcta gctgggggga tcgttggttt agctaccttg
    29341 attcaaggta tttcctttgg tccagcatta tcagcagcat tagatgtgaa tgttccttca
    29401 aatgtcgcag atgcaatcaa tagttctgag attggtgcaa cgattgcaga tggcacactg
    29461 tcagttggat tatggggttg gctaaaacta gatcatttga atggaaacgc ttattttaca
    29521 gttatgatca tgggagctat ttcagtcatt attttttgta aattgatgct tgccaacatt
    29581 acgatcaaaa tgcctgattc agtaccacca gcagtatcta aagcatttgc agccatttta
    29641 ccagcgacga tcgctttata cgtcattgct attatcaatt atatcgtagg tcgtgtagct
    29701 gatgggcaac tgatcattga tttggtgcaa aaatatattg cagaaccatt cctaggatta
    29761 tctcaagggt tgggagcagt attgatcgtc acagtatttg ttcaagtatt ctggttcttt
    29821 ggtattcatg gatcaaatgt attggcacca gtgctagagg gaatctgggg acaagcacaa
    29881 ctaatcaata ttgatatttt ccaaaaaggt tataacggaa aaactggtac accagccgtg
    29941 ttagatgcaa tcgaagatgg aaaagcttat atgtgggtcc gcggatcgtt cgatgctttt
    30001 gcatggtttg gcggatcagg cggaacgatc gtcttactga tagctatctt gattttctct
    30061 aaacgtgcag attatctaac agtagctaaa ttatccattg ggcctggtat cttcaatatc
    30121 aacgaaccag tgatgtttgg tctgccaatc gtattgaatg caatcatgtt tatcccattc
    30181 ttgattgctc cagtagtagc aacagcaatt ggatggacag ctacttatct tggcctagtc
    30241 gctccggtat cgcagcaagt cacatgggta gtaccgccgt tcttattgtc attcttagca
    30301 acaggagcgg attggcgggc accgatcgta acattagtat gtatggtcgt gactttcttg
    30361 atttgggcac catttgtcat ctctgcaaac aaaatggacc ctgccgcagg cgaacaacaa
    30421 taagatgaac gtgaggagcg ggacgcttta agtttcgttc ctcataatga attttcgagg
    30481 aggaactgaa atgattacaa ttgccagtgc aatgcaacaa gatgaaatca aagaattgtt
    30541 agaaggatac aatgaagaag gaatgacttt tacatttaaa gaaaaacaag gaatcaagct
    30601 gttctttgaa acaagtgcag aagatccgga agttgcggct aaaaaagcaa aggaactaat
    30661 caaagcacag ccttggggaa cagtccttta ttttcaatca acagctgttt agaaaatggt
    30721 ttaaaaagat aggatggttg agatgataaa aaacggttta tttttatgta gtattggttc
    30781 tttgatcgtg ttgtatagtt tgaatccaca ttcgcaaagg tatgatttgt atagtatggt
    30841 cacaggtctt tttctaacag gattaggaat ctatttcttc atgaagggga aaaaaagaga
    30901 agaagcaaag gagaaaaata actaatggaa atcacgaaaa aattaaatat tccagcatcg
    30961 tttttttacg ataaagttat ggactctgtc cttttcgatg tacgtaaagc gaccggcaaa
    31021 tcagtaacta gaaaacagct gaaaaatttg gagtacgtca aacaattctc gcaaaataac
    31081 cgggctcgca taaaaatcga agaagtcatt gaaaaccagt cttataagtt tcgtacatcg
    31141 acaacgaaga atgaattcgt cgttcattat caaatcaaac cattggacga ttcgacctgt
    31201 gaggttcatt acacagaaaa aatggagtct tatggcatgc ttcaaaagtt gaatgatatg
    31261 cttttaggaa caatcttgat gtattttaaa cggagacgtt tcaaaaaaat gctagatatg
    31321 atcgaagcaa cgtattaaat caatgaaaca tggtactatt tagttattga aaaatgaagt
    31381 aagctagaag gaatttttca taggaagata gatctttcct gcgaatcctc actagctttt
    31441 ttgtaggaag gggcagcaaa atgacaaaca aagtaagaga gctaggtgta tcttcgattg
    31501 aggaaatgtt tgatttagcc acttatgcat ttaatggggc agatacacct gaaaggcgag
    31561 aacgttttcg tacacttgcg gagaattctt ggaattacgg attttttgat gaggaggatc
    31621 ggctaacaag tcaagtcatg gcgacccctt ttccagtgaa tttttacgga caagagtacc
    31681 taatggccgg cattggctat gttgcttctt atccagaagc aaggggacaa ggcgggatca
    31741 atcagatcat ggaaaagatc cttgaagatt gccgtgatcg taaagtaagc ttatcttatc
    31801 tggcgccatt ctcctatccc ttctatcggc gctatggcta tgaacaatca tttgataaaa
    31861 tcagttatca actaaacagt cgtgatgtgc cgtttatcaa aaaaaaatct ggaaaaataa
    31921 agcgtatgga ttttgaagat gcaaaagctg caatcagaag aatttatgaa cagatgccgg
    31981 aaaaccaacg agctggtctg aagcgcgaag actggtggta tacgtataaa ttcaagcaaa
    32041 aaggcaatta ccagtttgct ttgcattttg atgataatga ggaagcaacg ggatatatcg
    32101 tttatcagct tgcaacgtct tcctttatta ttgcggagtg gggatttttg gatcatgatg
    32161 ctttttgcag ccttgttcgc ttcgtaagtt ctcacaatgg tgcttttgaa acatttgaat
    32221 attcatgcgg ctacggcggt aatgatcaaa attatctatt agaaaatcca tttgcttctg
    32281 taatgatcac accttatatg atggcgagga tcgtagatat ttctttgttt ttggaaaatt
    32341 atccatttaa aaagagacaa gctgcatttg ccctacaaat agaagaagat cattatgcga
    32401 aatggaatga aggaatcgtg gaagtaacaa tcgaaaatgg aaaaaatcat ctctcaaaag
    32461 tagaacaaac aaaactccca atcgttcgag gaagtatcca acatatcacg cagctattat
    32521 tagggtatcg taaagtggaa gagttggtat tccaagaaag aatacaggtg acaaaagaat
    32581 atgaagaaac gttaagctca atcttgccac agcaacagcc aattcttaat gactattttt
    32641 gataaaaaac gggtgatgac aatatttttg tcaccaccct tttgtttttc atttggcatt
    32701 tgaaggaaaa tgttaaagta aatcctcttt tttcgtaaat ttgaattttt tgttttcttt
    32761 tgttttaggg ttttcttttt tgtggtcttg gattgcttct ttcgtaatct tttcccgatt
    32821 ttttttagat gaatctaagc gttctgcgta tactttttct tttggcttat cattcattgg
    32881 aaaggacctc cctacattat ttcattctac ctctagttta tcatctgtta aggactagtg
    32941 gaaacgtttt gcctaactta taagctgatt tttttagcag ataagcttgc aagtaaaaaa
    33001 caaacaagct acaattgatt tattgaaatt gttcctgtag ctcagtagga tagagcaatc
    33061 gcctcctaag cgataggtcg ctggttcaat tccagtcagg aacgtataaa taacccctgt
    33121 atcctttgcg gtacaggggt ttgttttgta tttgtgccaa aaatgtgcca aactagttat
    33181 catttttata taaactatcg aacacagtat tgattttttc tttatcctga tcttcaagct
    33241 ctcgaacaat atgaagataa gtagacattg tagtttctaa actgctatgt cctaaacgtt
    33301 tcgatacgct aagtatattt actccttgat aaagtaaaat agatgcatgt gtatgcctta
    33361 gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg cgcaaatatt
    33421 tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca aaattatgag
    33481 ggtttttgat ttcaaaattc tcgaataatt ctttttgatt gattttaaac ttttttaaga
    33541 gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt tttgtttttc
    33601 cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga tcttcgaaat
    33661 cgatatcttc ccatgttaat cctagaagtt cagcaaatcg cattccagtg gcaccagcaa
    33721 ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg agtttcatta
    33781 aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat gaatcttttc
    33841 ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct acggcatctt
    33901 tcaacgacgc tctaatatgg ttattaaact taataacggt agattttaca tggtctttag
    33961 catacttatt taaaaagcgt tgatatccca ctctatctaa atcagaaatc aataccgctg
    34021 gcatatattt ctttatgttc attaacgtat cttcgtattt tctataagtg ataggagata
    34081 ctgttccttc tttataaagt tgcatccaat cctcaaagta atctgataat aatagatttt
    34141 tgcgatcccc tttaaggcct ttgttcaagt tatattctaa ttcgttggcg gcatcttttg
    34201 cttctccttt tgtcttaaat cctgattttc ttatcttcgt gtattttccg tcatcttttt
    34261 tgtatgaaat ttcgtattgc caactatttc cacgtttgac taatcttgcc ataattgaat
    34321 cagctctctt tctctgatac aataggaact ataaagaagc ccattgtttg ggttttatta
    34381 ttttttgcac atcctttttg cttgccggcg caaggatgtg ctatttgtcg ttttaatggt
    34441 ataattggat gtgtattaga tcatccaatc agaggtgttt attatgatta aagtgatttt
    34501 taaaagaata actgaacttc cagagtttag attatttgtt gattattggt atgtctggtt
    34561 ttttattgtt gcattgctga taggtctaga tgtcatatta gaaagaaaac ttaagtaaga
    34621 gttgagttaa taaattgaat tcctaaatga gatattttgt agcccatatt ttgtttgccg
    34681 acatcaccta atactatgga gctaatcgtt ttttctaaca gaccaaaatc agataatcta
    34741 tttaagctac catctattag ctcttgtgaa tagccagaca tgctgatatt tccagtgaaa
    34801 ttttcgtttc cacgactcat taagcatgct aacaattgaa tctcaatcga tgttaattga
    34861 ttcagtacaa gaagataata ctcctcgtta tcccatgaag cgttgttaat tcgaagtaaa
    34921 caattcttat aaagattttt atacatatct attttatttt gtgcctttga tttttctacc
    34981 tcgtcattta tcccttcgaa tattccaatg agttggtcct tgttctctaa gttgaaaaca
    35041 tcttctggaa tttgatcgcg aatgttttct aatgattcgt ttaaattttt gtagaatttc
    35101 tcgatgcgtt tgaaacgctt ctcgttttta gacccaaaat aaagtgactg cacaacgcca
    35161 ccaatataag gaattgctcc aataccgcct tcaactatta attcaagata ttcttttttg
    35221 gttagttttt cattgctatt atccatagtt acctcctatt ttgtcatttc ctttacaagc
    35281 attgtgactc ctgatggcac tcttttgtat aggttttatt attgtgcaca atctaactct
    35341 tagtggggcg tggttcattt attttttact tactttacta ttaatcatat cccattgagc
    35401 ataggatttt tctatgttcg atcctcttga actaatttct cctgtaaaag ttactttatc
    35461 gccctttgta aaggtatcag cattgtttaa gtccttcgca aaaattacat atgaaacttt
    35521 aggtgttgca gaaatatcat tccaactttt atcatcgtac tctttgtctg ctataatggc
    35581 tattctagta gtcattgatt cgataacagt tccttcaaaa gtatatgttt taccactaat
    35641 atatttagta aaagctgtat tttgctcagt agctggagta atagaataat aactatctaa
    35701 gaaagcaccc cagtcatgat tatttgtctc taaaaattct tcaaattgag ttaggctttt
    35761 attgctgctg ttagatgtag tagtttgagt atcagatgat gagctttcct gtttgttatt
    35821 gtcatttgcc tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa taatcccgat
    35881 aataaggaga atcataggtg ttttcttatt tgtttttttg ataaatctta caattaacat
    35941 gattaaacct ataataaata aaacaatacc agtaaaacca aatataccgt ccattaatag
    36001 atcctctttc tcttttgata ttgaatatta taaaccaagc aattcttttt tcttaaaatc
    36061 gaattcttct tgattaataa tcccttcatc taacaactct ttatactttt taatttcatc
    36121 tgcacttgaa atttgtgttt ctttggaatt attatctaat atagattgtt gcttctctat
    36181 atatttttt
//
LOCUS       DAZAID010000018        34806 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000018 DAZAID010000000
VERSION     DAZAID010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.018"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(457..2349)
                     /locus_tag="ACG9BE_000852"
     CDS             complement(457..2349)
                     /locus_tag="ACG9BE_000852"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3031624.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2346..4073)
                     /locus_tag="ACG9BE_000853"
     CDS             complement(2346..4073)
                     /locus_tag="ACG9BE_000853"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3031625.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4089..4739)
                     /locus_tag="ACG9BE_000854"
     CDS             complement(4089..4739)
                     /locus_tag="ACG9BE_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFN3031626.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4943..5239
                     /locus_tag="ACG9BE_000855"
     CDS             4943..5239
                     /locus_tag="ACG9BE_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN3031627.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5431..6051
                     /locus_tag="ACG9BE_000856"
     CDS             5431..6051
                     /locus_tag="ACG9BE_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN3031628.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6143..7309)
                     /locus_tag="ACG9BE_000857"
     CDS             complement(6143..7309)
                     /locus_tag="ACG9BE_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN3031629.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7321..7674)
                     /locus_tag="ACG9BE_000858"
     CDS             complement(7321..7674)
                     /locus_tag="ACG9BE_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFN3031630.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7802..8824
                     /locus_tag="ACG9BE_000859"
     CDS             7802..8824
                     /locus_tag="ACG9BE_000859"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFN3031631.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8843..9004
                     /locus_tag="ACG9BE_000860"
     CDS             8843..9004
                     /locus_tag="ACG9BE_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031632.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9263..9697)
                     /locus_tag="ACG9BE_000861"
     CDS             complement(9263..9697)
                     /locus_tag="ACG9BE_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031633.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9807..11666)
                     /locus_tag="ACG9BE_000862"
     CDS             complement(9807..11666)
                     /locus_tag="ACG9BE_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN3031634.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11667..11807
                     /locus_tag="ACG9BE_000863"
     CDS             11667..11807
                     /locus_tag="ACG9BE_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFN3031635.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11784..12944)
                     /locus_tag="ACG9BE_000864"
     CDS             complement(11784..12944)
                     /locus_tag="ACG9BE_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN3031636.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13251..14675)
                     /gene="araA"
                     /locus_tag="ACG9BE_000865"
     CDS             complement(13251..14675)
                     /gene="araA"
                     /locus_tag="ACG9BE_000865"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFN3031637.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14691..15389)
                     /locus_tag="ACG9BE_000866"
     CDS             complement(14691..15389)
                     /locus_tag="ACG9BE_000866"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFN3031638.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15346..17007)
                     /locus_tag="ACG9BE_000867"
     CDS             complement(15346..17007)
                     /locus_tag="ACG9BE_000867"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFN3031639.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17225..18625
                     /locus_tag="ACG9BE_000868"
     CDS             17225..18625
                     /locus_tag="ACG9BE_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFN3031640.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18877..19956)
                     /locus_tag="ACG9BE_000869"
     CDS             complement(18877..19956)
                     /locus_tag="ACG9BE_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3031641.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20042..21001)
                     /locus_tag="ACG9BE_000870"
     CDS             complement(20042..21001)
                     /locus_tag="ACG9BE_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFN3031642.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(21013..22524)
                     /locus_tag="ACG9BE_000871"
     CDS             complement(21013..22524)
                     /locus_tag="ACG9BE_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFN3031643.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22546..23109)
                     /locus_tag="ACG9BE_000872"
     CDS             complement(22546..23109)
                     /locus_tag="ACG9BE_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN3031644.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(23115..23978)
                     /locus_tag="ACG9BE_000873"
     CDS             complement(23115..23978)
                     /locus_tag="ACG9BE_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3031645.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23992..24888)
                     /locus_tag="ACG9BE_000874"
     CDS             complement(23992..24888)
                     /locus_tag="ACG9BE_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3031646.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(25063..26352)
                     /locus_tag="ACG9BE_000875"
     CDS             complement(25063..26352)
                     /locus_tag="ACG9BE_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3031647.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26324..27841)
                     /locus_tag="ACG9BE_000876"
     CDS             complement(26324..27841)
                     /locus_tag="ACG9BE_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFN3031648.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            28039..28929
                     /locus_tag="ACG9BE_000877"
     CDS             28039..28929
                     /locus_tag="ACG9BE_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFN3031649.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(29002..29826)
                     /gene="nadE"
                     /locus_tag="ACG9BE_000878"
     CDS             complement(29002..29826)
                     /gene="nadE"
                     /locus_tag="ACG9BE_000878"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFN3031650.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29838..31310)
                     /locus_tag="ACG9BE_000879"
     CDS             complement(29838..31310)
                     /locus_tag="ACG9BE_000879"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFN3031651.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31947..32309)
                     /locus_tag="ACG9BE_000880"
     CDS             complement(31947..32309)
                     /locus_tag="ACG9BE_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFN3031652.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32532..33284
                     /locus_tag="ACG9BE_000881"
     CDS             32532..33284
                     /locus_tag="ACG9BE_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN3031653.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33424..34347)
                     /locus_tag="ACG9BE_000882"
     CDS             complement(33424..34347)
                     /locus_tag="ACG9BE_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3031654.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34362..34634)
                     /locus_tag="ACG9BE_000883"
     CDS             complement(34362..34634)
                     /locus_tag="ACG9BE_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031655.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10276 a         7232 c         6010 g        11288 t
ORIGIN      
        1 aagcttttga acaccgttta aggaagaggt tgtgaaaaag tcgcttagtt tcgagtagta
       61 agaaaaattt tgtgaaaatg gctttctaca tttttgcata aatttggctt aataccgaga
      121 aactggcttt tgaacaccgt ttaccaagag gttgtgagcc tgccgtttaa ctccgagtca
      181 caaggagcaa tttcaaaaaa cagcttctca ggtttgttga ctattgtgac ttgtgaccga
      241 ggagttggca ggagaacacc gtttaaggaa gaggttgtaa aatcagcggt ttgacctgag
      301 tattagagat acaaaacgga aaaatagctt ctcatatttt gccgtttttt gagctaatac
      361 cgaaggacag ctgattgaac accgtttatt cggaaaaagg gactgtgaca cgacttttgt
      421 cacagcccct aaaaaaaata ataaatttgt actattctat gaagcttctt cttcagaaaa
      481 ctgactgtta taaagatctg catagaatcc gtctttttcc atcaatgtat cgtggtttcc
      541 tgtttctacg atcgaaccat gattcatcac aataatgttg tctgcatcgc gtattgtaga
      601 caagcggtga gcaacaacga aacttgtccg gttttccaat aggcggttca tcgctttttg
      661 aatcagtatt tctgttcgtg tatctacact agatgttgct tcatcaagga tcaggacatc
      721 tggatttgct aagaatgccc gcgcaattgt gattagctga cgctgccctt gcgaaatatt
      781 gcttgcatcc tcgtttaata cagtgttata tccttctggc aatttacgaa caaaatcgtc
      841 tacgtgagca gctttagcag cctcaatgat ttgttcgtct gttgcatcgt cgttcccata
      901 tttgatattg tcatagatac ttcctgtaaa cagccaagta tcttgcagta ccattgaaaa
      961 ctgtgcgcgc aattcatcac gtgaaagatc acgggtatct acaccatcgt aacgaatact
     1021 acctccagaa acatcgtaga accgttcaag aaggttgatc agtgtcgtct tccctgcacc
     1081 agtcggacct acaatagcga ccatctcacc aggtttgaca tccaagttaa aatcagtcat
     1141 tagcaagtta tctggtgtgt agccgaattg aacattttca aaacgaactt tgtatggtgt
     1201 atctttttct tctggaaggc cagatttttc attggacatc tcttcttcat ccaacacttc
     1261 aaatacccgt tcggctgaag caactgttga ttggatagtg ttcatcaaac ttgcaagttg
     1321 agtgattggt tgagagaact gtgttgtata ttgcaagaac gcttgaacat cccctaatgt
     1381 catgttccca tttgcaactt ttactccgcc tagtacagcg acgaacacat agcctagatt
     1441 tttaacgaaa ttcatcaacg gcatgatgat tgccgaaata aattgagctt tccaaccagc
     1501 ggcatacaga ttctgatttt ctttttcaaa tacttcctgg tctttttcag tatgattgaa
     1561 tgttttaaca atggtatgac caccatatgt ttcttctact tggttattca ataaacctaa
     1621 acttttttgc tgatctgcga aaaacacttg tgatttcggc gctacgatca tcacgatgat
     1681 caagcttaaa gggatcatca ataacgcgac taacgtcaat tgccagctga ttgaaagcat
     1741 catcacgatg accccaatca atgtgactgc actagttaca aattgagtca aattttgctg
     1801 cagcgtgctt gcgatgttat ccatatcatt gattgctcga gacatgatat ccccatttgt
     1861 atgtgtatca taataggaaa tcggtacacg attcattttg gcttccaaat catgacgtaa
     1921 ttcataaact gttcgttggg aaacacgtgt cataataaat tgctgtaaaa agttgaatac
     1981 agcagaaatc aaatacatca cgattactgt cgcaatgatc tgcgcgattt tatcaaagtc
     2041 tatcggaaat tttgttactt gttggccttg ttgcatctgt tgagcgccag ccattacacc
     2101 tttgaatatt tctgtggttg ctttccccaa gacctttggt gtttggattt ggaagatcac
     2161 ggctgctatt gctagtacaa atacagcgat gatcgcaacc atacgtttag acatatagcc
     2221 aaacaatcgg cgtaccgttc cccagaaatt tttaggcttt tcacctttta caccgagatt
     2281 tctgccaggt ccaccatgtc ctggaccgcc tgctggtcca tgagagggac gtgtgtttgt
     2341 tttactcatg ctaagtcctc ctctcttagt tgtgaatgaa cgatttcttg ataagtttcg
     2401 tttgttgcta atagttcttc atgtgttcct ttgccaacca gttttccttc atctagcaca
     2461 aggatcatat ctgcatccat tactgtactt actcgttgag caactaaaac ggtgatactt
     2521 tctttcatat tcgttttcaa tgcttggcga agattggcat ccgttttgaa atccaacgct
     2581 gagaaggaat catcaaacac atagatatct gctgttttga ctaatgcccg tgcaattgcc
     2641 agacgctgtc tttgtccacc agagaagttt cctcctcctt gttccacatg gctatctaag
     2701 ccatcttcca tctctgaaac aaaatcttta gcttgtgcga tttctaatgc tttccaaatc
     2761 atttcatctg ttgcatccgg cttaccatac tgcatattgt ctcggatcgt tccagagaac
     2821 agaactgctt tttgaggaac aaagcccatt aattctcgca aattatattg agtcatgtca
     2881 ctaatgtccg ttccatttat ttggatcgaa ccagattcga tgtcatataa tcgagggatc
     2941 aaattgacta aggtcgtttt acctgaacca gtccccccta tgattgcgac gatctcgccc
     3001 gacttggctt ggaaatcaat atcttccaat gcaagttttt ctgctccatg atatcgataa
     3061 ttgacatgat tgaaggccaa tgttgcgttt tttccagcaa aagaaactgt tttaggattt
     3121 tcagggtcat ggataccgat tttttcatcc aacacttcat taatacggtc agctgaagct
     3181 tgtgcacgag gcaccatgat aaaaatagcg gaaagcatca taaagctgat caagatctgc
     3241 atcgcataag tcataaatgc aatcagatta ccgacttcca aagtcatatc tgctatgtag
     3301 tgtccaccaa accaagtgat tgctacgttt gtaccgctca tgatcaatgt catcattggc
     3361 atcatcaatg cgacgatcgt attaactttg attgccgtat ttgtataatc tttattcgct
     3421 aaatcaaaac gattttcttc aaagcgtgtc ttatcgaatg ctcgaatcac tctgacccct
     3481 gtcaatcctt ctcgaaatac caggtttaaa cggtcagttt tcttttgcat acttttgaat
     3541 aatggaacag caaagtacat gattccgccg atcaaaataa tcattactgg gatgacaact
     3601 aagaagattt ttgtcaactg atgatctttt tgataagcaa gtatgctcgc gccaatcaac
     3661 gtgattggtg cattgatcat catacgtaaa aacatttgtg taaccatttg gatttgagtc
     3721 acatcattgg tcgttcttgt gatcaaagag gccgttccat atttatcaaa cgcatctttt
     3781 gtcagactct ctgatttttt atagacatcc gtacgcaatt gcttacccag tttctgtgat
     3841 tcacgagtag caaaaaatgt atttgcgata gcagctaaga tactgatcaa agaaaagccg
     3901 atcatcacaa aacctgtatg ccaaatataa tcgacatctc cttgcgctac ccctttatca
     3961 ataatattgg aggtaagtgt cggcaaatat aaatctgcca gtacttggat caccatgaaa
     4021 actgcagctg caattgctga agtcaaggag atccgtttta cgatttttag catctagttc
     4081 ccttctttct atttatatac tccgtttttc agccaagaaa gtttcaaatt gaattcactg
     4141 attgcttgct ctactgagta atcttcagat gttgaaaata agcgatacga atgcgcaatt
     4201 gttgtaaata cagtattcat cactaattgg atcagtaatt ccaattcatg atcatttttt
     4261 actttcagct tcgtccgatc gatcaattca agcaactctt ttgcatcttg tttgtgttct
     4321 ttatgtggat cgtgaggcgt tttttggcga ctcgtatgag gagcgacttt tcttgaggca
     4381 cggtaatcca tgttcataaa caagttacga taaaaagaag cattctcgcc ttccaaaatc
     4441 tcataaatca ttttagaaaa ataggattcg aatcctttga atagatcacc atcggattct
     4501 ttcattagtt gtaacatatc tttagagctg tcacgtctca ttgtctcaaa gtaataaaaa
     4561 taaagatcct ctttatcttc aaaatactga tagaaactgc ctctagggat atccgctaat
     4621 ttgacgatat tagcaatcga agcttctttt aagggtactc tagaaaactc gattctcgca
     4681 gcctcgatta gtcttttttg tttttcttta ggcaaatgga aaaatgtctg tgtcggcata
     4741 tggattctcc tttctttcaa gatgacacat tgtcatatga cgctatgtca ttataacacc
     4801 tcctacctgt tttgcaagac atttgcatag tttttttcat tatcatccga tacattaaaa
     4861 aagtgataag aggaatcctt ccatttgaat taatgagagt tgcccattaa aatagccaca
     4921 tactaaacaa aggatgagat aaatggccca atcaattagt gtccaagact tcaataaatt
     4981 gcctctcgat gctgacagca tcgttttaga tacaagaaat accttagatt acacaaaaaa
     5041 ccacttaagc cactcgcttt ctatccccgc tgctatcttg ccaagacaat taaaaaatct
     5101 agatccaaag aaactttatt atattctaag ctatacaggc aggcgatcag aagcattagc
     5161 cgatcaactt actgctaatg gttttcgtgc tgtttatatc attggtggga tggaagctct
     5221 tcagtcgagt gttgcttgaa tattgcttgt tagattcaaa agaggctgtg acaaaaaatc
     5281 gtgtcacagc ctcttttata ccctgtcttt ttttagttat ttattcttca accttatcaa
     5341 tatgacttga tagccttttc tttttcgagt aaactgattt tgttagtcat agttataaca
     5401 atcatagcaa taaaaggaga aggtaagcat atggcatctg ttttagtagt caaaggtcat
     5461 cccctaactg cagaagaatc gcgcacagtt aaggcattga cgtctttttt aacaagttat
     5521 aaagaaaatc atccagatga tgaagtgact gttttggaac tttatcgcga tgatattccg
     5581 gaaatcgatg aagaattatt atcaggttgg gaagcattac gtgctggtgc agaattcact
     5641 tccttatcag aaagtcaaca acaaaaaatt gctcgtttca atgaattgac tgatcagttc
     5701 cttgctgctg ataaaatcgt tatcgcaaac gccttatgga atctaaatat cccaacaaaa
     5761 ttaaaagctt ggttcgatac cgtcaatgta gctggaaaaa cgttccgcta tacagaaaac
     5821 gggccagaag gattagtgac aggtaaaaaa gcactgcaca tccaatctaa tggcggtgta
     5881 tacaacggac aagactttgc ctctcaatat gtaaaaggta ttttaaactt tgtaggcatc
     5941 gatcaagtag atcagctatt catcgaagga atcgattatg accctgaccg tgcagatgag
     6001 ttgatgcaaa atgcattaga taaagctgct gctttaggta aatcatttta aaaattgaga
     6061 taaatgcata caaaagagac agaggaaaaa atcttcaaag cagcatttgt gccttggata
     6121 ttttcccatc tgtctctttt tgttattctt tctgcttttt caacggattt tcttcataaa
     6181 gagaagagat tgttgtgtac cattcaaaaa catgagtaat cactacttta gctgctgcat
     6241 aaacaggaat ccccaagatc acaccgatga ttccgaacaa tttcccagaa gtcagcagaa
     6301 ctaataaaat cgttactgga tggattgcta actgattccc tagtactaat ggagagatca
     6361 agcgtccttc tattgtctgc tctactgcaa aaacgatcag tactttgatg atcatgaatg
     6421 gacctgctac aatggctaaa aagatagccg gaatcatcgc taaaaatgat cctaagtaag
     6481 ggatcaggtt taaaaaaccc gcaataatcc ccaacgtgac agcatactct aaaccaatga
     6541 ccgcaaaacc aatcataaac atgattgcca cagcaaaagc cacagttagt tgtcctctta
     6601 tataagaaga aacttgacta ttcatttcac tcagcacatt caacgtcggt ttttgcatgc
     6661 gtgtcggtaa aaatttgaca aaataaggtg ctaattgttt accgtctttc aatagataaa
     6721 ataagataaa cggcatcgtc agcagagcca ctaaaatagt cgctaccgcg ccgacaaaac
     6781 ttccaagact ctgaactgta ctcttagata tatcttggat catatcgcct gccgaattca
     6841 tgaacttatc acctgctgtt tccaactgtt cgcgaaattg gttgaacagc gggtcccgca
     6901 aaatctcggt taatttatta tcaatcgtat ctacatattg agggaagtta ctgatgaagc
     6961 tcactgtttg ttcctgaatc ttagggatga tcactacgct cccccatgcg attagtgcaa
     7021 caaccaaaac aaacagccca atgatactgt aaagccttgg tacttttcgc ttttcaaagt
     7081 aatcaactac cggattcatc aagtaataca agattcctgc taaaatgata ggcagaccta
     7141 cgattgctaa aaattgccag attggtgcaa acaaatatga aacttttgta aataaaaaga
     7201 cgatcagcaa tattaacaat acaacaagta aggctgtgac tacttgatta tttaaaaacc
     7261 agcgccaaaa ccacgatacc ggtctgttct tcttattttt tccttccata attcctcctg
     7321 ttagttataa aaaatttggc ttcccaaacc aatagcaggc agtctatgcg gatgaagata
     7381 gattccattt gcaatttgtc cttcttctgg acaagcatca aaaatcaatg atacgtgagc
     7441 tactgtcttt aaattcccat tggccatcgg accgacataa tcgatcgtat atacctgatc
     7501 atcaaaagca attgttccgc ctgctttcaa agaaaaaggt tcgtcttttg atacttcttg
     7561 aatcaccgaa tattttcgta aggcagctgt tgctgtttta tcgaacaaaa tcagcatggg
     7621 ttcttccttg tttattgctt ctgtcccaat ttctgtaatc tttgcctgaa tcattgcact
     7681 ctctccttta attgctttca aaaacagtat aacacattct cattctttag ccgtgtggaa
     7741 taattatgtg atagaataaa aacgaaaact atttcattac aattaggagg aatcagctca
     7801 tatgatcgaa aagataattt taggaaccta tacgcgtcgc gaaagtaaag gaatctatac
     7861 gatcgacctt gatacagaaa aagaagaatt agccaacctt tcattagctg ttgaagaaaa
     7921 tagccctacc tatgtagcaa aaagtaaagc tggcaatctt tacacagtta ctagtgtgga
     7981 tggattaggt ggtgccggtg cgtacgataa ggattttcat tttttgaatg cggtaacaga
     8041 gtctggcgct cctttgtgtt atgtagcggt tgatgaagca cgtcaattac tatacggggc
     8101 gaactatcat aaaggtgaag taaatgtcta ccatatatta ggagatggcg aactgaaagc
     8161 agcagattca atattccatc aagaagcgac aggtcctcat aaaaaccaag atcatgcgca
     8221 tgtccactat acggatctga ctcctgacca acgattagtc gtatgtgatc ttggtacaga
     8281 tcgtgtgtat acttatgatg tgtccgaaaa cggcaaattg aatcttgtaa ctaccttcac
     8341 tgcagagcca ggaacaggcc ctcgccatct tgtctttcat ccaaatggac aattcgctta
     8401 tttatttggt gaattagaca gcacagtccg cgtgctttct tataataaag aaacaggtgc
     8461 ttttgaagaa ttacagaaaa tatcgacctt gccagaagaa ttcgacggtg aaaatggagg
     8521 ggcagctatc cgcatttcaa atgatggcaa gttcctttat gcttctaacc gaggacataa
     8581 ctcgattgct gtattcgcag tttttgaaga tggttcccac attcaaaaca agcaaatcat
     8641 ctcaacagaa ggggattttc cccgcgattt cgcactagat ccaagcaata attttgtcgt
     8701 gtgtgccaat cagaacaccg ataatctgac attatatcgc cgtcatcctg aaacaggatt
     8761 actggaatta ctgaaaaaag atgtctatgt accagaatgt gtctgtgttt attttgatcg
     8821 ttagtaagaa aaggagaatg aaatgaatca gcaagacgta ttagacagac tacgggaaga
     8881 actaaatatt cctttctttg atgggaaaat cgaagataaa gagtatagcg aagaggaata
     8941 tcagaaactc aaagccgatt tacagaacta ctttgaacaa tatgtacaga atgtagaaaa
     9001 ctaatcagct ttccatacta tctgaaaaga acatcaggac aagacttttc cgtctatcct
     9061 gatgttcttt tctacaaata aaaaaccgcc gagatggtta gactcgacgg aaaaggagta
     9121 tttactcgat tttgagtaag tacaaggttg ttgttggtta taggtatgtc attatcataa
     9181 aggattttga tcactctatc ataaaggtaa tattacattt atattagata actctagttt
     9241 ttattgttat caaagatttc ttttattttt tcaacaacaa attagtcatt ttttggatgg
     9301 cagctgtcaa ttggtctgtc gcttggtctt gttttgcttc agatgtattg aatagatctt
     9361 catctacccc tgattcaaga tcgatacttt ggatcatttc ttcacagcct gctacatatg
     9421 aaagataagc tttttcaaaa tttttatgaa tgcccattgc ttttgctggt ggacgcaaag
     9481 tactgacttt cataagcatt gcccgatatt tttttgttcc ttcttcaaat aacaggatga
     9541 tctcttcaag agtcgatttg tccagttcac ttgtttttcc ttcatcaatt gcgtttctga
     9601 tcacttcata tttcggatgc attccttctc cgattgcttc tgtttccttg acgatttgat
     9661 cgatggactg cacataatag ccaatattcg gtaacatgtt cgttttccta ctttctccat
     9721 tgttgttatt atttattcta cttgttttta tcctcaaatg acaagtactt gaaaaaattt
     9781 aatggtttct atacagctcg ccattcttat tcgtgctcaa agaaaccatc tgctaaacta
     9841 tattgtttcc ctgcgacaat gtcatattgg atcaattgat attcgtcgta aatctcttgt
     9901 tgcgagcgat tgaattgcgc ttctttatac cactgtccgt tttgatagta cagttctcgt
     9961 tcaaacgccg gaagttcctg ttctaatgat aaaagcagct gataaaagcc atttgttgtc
    10021 tgattcgtct gttccattag attcgctgca aaataaaacg gactggtgat tgcatcgtga
    10081 gtcgtttgct tttctaattc tcctttactg tcgaacataa gaaattgtgt ttcatgcaat
    10141 gtttgtttgt cattttttgc ttggatcgta tctgaataga tccctggaag atgatccccc
    10201 caaaaaacaa ctaaagtcct tctagaaaga ttcttcaatt cttccgtgaa ctgcttcagt
    10261 gccgtgctgc tataggaaat atcttgcaga taattctcta agtcaagtgt cccacttcca
    10321 tctgaagttt ttgcaggata gtctagatta tcatactttc catcgtaggg catatgtgtc
    10381 tgcatcgtga ctaaatgaat gaactgaggt gttttttctt ccttgagaag tgtcattact
    10441 tctttgtaag ctgactcatc agaaatatat ggattattct ctatcgtatc cgtataggtc
    10501 atcgtacgct cgctgatgaa ctggtcaaat cctaatgtct gataaacatc ctctctttta
    10561 tacatcgaag tattgtatgg atggatcgct gtcgtctggt atgattgtgc gtttaacgct
    10621 gaaacgatag aaggaagctg atccattttt ggtacaagca tcgtgtaagg ggtggtgagt
    10681 tgcgcattaa ataacgccat agaaaaacca gtcaatgctt caaattcgat atttgctgtt
    10741 ccaccgccat agttttgaga aagcatattg ccactatatg tttgatcagc tacttcataa
    10801 taatctgcca atggctcacc agatacttct acgccgttta atcgactagg atctgagaaa
    10861 ctttcactca tcacgaacac gatgttcggc gactcttcct ctacagtttt atttttttca
    10921 tcggctagct tctggtattt ttcagtgatt tctttgatct tttctttcga atatccttca
    10981 ggttcatcca ttggttctac cttcagattg tacaaaaacc caccgataaa accagtgtta
    11041 tagtagttca ttttctggct gtaaggtatc catagtgccg ttttattata tgcttttcgc
    11101 aacaaattat cgggattgtt gaaattactg atatacccta gcagtccaat tgccacaagc
    11161 agtgtcgtta atcggatcaa ttggaatcgc cttccttttt tcaggcttcg aaacatgtac
    11221 cagcaaaaca atcctatcac ggttcctgcg gcagctaaga tcactgtaaa cagcatagtg
    11281 ccggtcatat ctttcagcaa tccgatttca gtgatcatct tcaaatcgtc tggataaatc
    11341 ggttcttgcc gataactcat tttcatataa tttgcaaaac ctaatacacc gatggctacg
    11401 acatacgtaa gggaacctaa agggacagat cctgctagtg caatcagaaa aataagaata
    11461 atcagcaaga cgagacaggc taagaagaat ttttccgtat gccaagaaaa agcaaatttc
    11521 aacgcaaggt ctacggatag atcattttga caccactgca aaaataaatt actaaaaaca
    11581 acaagaaaaa agatactgat gattgctgct gttcctttta aaatcttttt ctgtttggtc
    11641 gaaagtctgc ttatatatct tttcaaatgc ttgctccttc atttctatca ataaaagaat
    11701 gttgcttctc ttattttagt tgtacgaaat tcgaaagtaa agaacggaaa gaagaattaa
    11761 tgaaatccaa atattctctt tctttatggc ctttctggtt tctttaataa gcggaccttt
    11821 cgtactccag aaattttttc tagctgttgt ttcacttgat tgagctgttc aaaatcagta
    11881 ctgtctatgt caatcaatat gtagacatgc tcattcttat ggttacgcga tatatctgcg
    11941 atcgctagct cgttctcatt cagcagagaa aatatctctg ctaatatatt ggttttcttt
    12001 tgataaaata tagtgaatcg aaacggcgct tggaaaaaca ttcgtgcgga tggataattg
    12061 accgcttctc taaccgtacc gaacaaaaga taatctttta aggcttcaac tgccaaacgt
    12121 ccgctgtctg ccaaagcttc ctgagtcgat ccccctagat gaggcaacat aagtatacgg
    12181 tcattttcta agaactcttc ttctggaaaa tcagtcaagt atttagccag atgttgtttt
    12241 tccaatgctt gcataacagc atatttatca acaatttcac ttcttcctac attgataagt
    12301 acagcatctt ttttcatttt ttctatattc gcctgatcga tcaatccttt tgtatcttct
    12361 gtcaacggca gcatgactac gatgtaatcc gaggtagaaa gaactgtttc taaatctgct
    12421 tgccgaacat aatccaactg ggcatcgcgg atagaataac ccaagacatc catccctaag
    12481 ctatagcaac ttagcgctac ttttgtgcct atcgctccaa gaccaagaag accgatggtt
    12541 ttgccttgca attctcgtcc cacataagca cttcttttgt tttctgcctg ttctaaaata
    12601 ttaggacctg ttagtgtttg taccatacga cttgcttcga ttatcggacg ggaagataag
    12661 agcaaacagc acaaaacaag ctcttttact gcattagcat ttacacctgg tgtattcatg
    12721 acgatcgtcc cgttttcaga agctttttca acgttgatcg tgttcacccc aacaccagct
    12781 cttgaaattg ctagcaaatc tggtgtaagc cattcatctt tcactttcgt actacgaata
    12841 actattcctt cggctggcaa atcatctatt attttgaaat attccccttg taacttttct
    12901 atctcttgtt ctggaatagc atcaatcaaa tgaacgtgat acataaaacc ccctccttac
    12961 taacaagtat ctcgaaattt ctgcaattag acaactgttt accatttgac ttcctagaag
    13021 caaaaagagc agttagagca gagtaggctg agtgataaaa ataaggctat gacaagttac
    13081 tgccataagc cttattttcg agagcaaaat attgagggtg agatatcttg ataaatggtg
    13141 ttccgttagc tgacattgat aataagacta aatttcaaaa aagttgaaaa gcaaatttcg
    13201 tcaatctctt cttatttctt aaaactaagt ggctttttca caacctcttt ttatctaata
    13261 accactgttt ccaactcaac taattttgcc cacgtttcaa tttgatctgt tgttagattt
    13321 agcgagacaa cggtatgatg accgccacct gcctgaatcc atgaatggac accttcatgg
    13381 aaattcggtt tagttttcca taacacacgt gcaacaggta aattaggtgc ttcagtcgtt
    13441 ggttcaaagg cttctacttc attgatcagc agtttataat gcgtaccgaa atctgccatc
    13501 gatacgacta ccccttctcc tgctttccca tcaaatacaa gacgcgccgg atcttctcta
    13561 tctcccattg ataaaggaga aacaacgatt cttggtttcg tcgcagcaag cgtcggatct
    13621 acttccatca tatgggattc caagattgct tcttggccac tagctagctc gtaagtataa
    13681 tcttccatga atcctgtatc tttccctcga gccatgattt tcagcaaccg atcaatcgct
    13741 gcagttttcc agtcgccttc cccagcaaaa ccgtatcctt ctgccatcaa acgttgtaca
    13801 gctagtccag gtagttgttt cattccatac aggtcttcaa aattagtcgt aaaagcgtta
    13861 tagcctcctt cttctaagaa acggcgaata ccgatttcct gctgagcttg tacttttaca
    13921 tgttcctccc atgtcttttc ttcataatca ccatagtcga aggtatacaa ttccttgtac
    13981 tcttcaaata attgatcgac ttcttcgtct gatacgcgat cgattacttg tacaagatct
    14041 ccgataccaa aataatcaac agtccaacca aattgaatct gtgcttccac tttgtctcct
    14101 tctgtgacac ccacgtttct catgttgtca ccaaaacgag ccacttttat tccaaaactt
    14161 tcattatagg ctactgcaac atccatccaa tcagcaatct ctttttggat ctcaggtcgt
    14221 ttccagtagc caacgacgat tttattttgt ttatttaatc gagcattgat gaacccatat
    14281 tcgcggtcac catgagcgga ttgatttaaa ttcatgaaat ccatatcgat cgttttccaa
    14341 ggaatgcttt cgttatattg cgtagctaaa tgaagcaatg gtttttgtag aagctttgtc
    14401 ccgcggatcc acatttttgc tggtgagaaa gtatgcatcc aagtgatcac acctgctact
    14461 tcttctcgat aattgacctc tttcatgatt tttgtgatcg tatcagcagt aactgctaat
    14521 tcttgaaggc ggattggata aggcaattgg ccattttcat tcagttcatc gaccatttct
    14581 tgtgcatgtt tctttacttc tcgcagtgct tcttccccat ataaatgctg tgatcctaca
    14641 acaaaccaaa attctttttc tccaatattc aacataatta gccactcctt ttatttgttt
    14701 tgtccgtaat acgcattttt tccgtgtttt cgtaaataat gtttatctaa aatccgttga
    14761 ggcagctctt ctgaaaatga attgagctgt ctggtaaata aattcatctt gcatacttca
    14821 tctagtacga ctgcgttcat taccgcactt tgcgcatctt tcccccaagt aaatggaccg
    14881 tgtccgtgta aaagtacccc tggaattgct aagggatcaa ttttgcgttc tttgaatgtt
    14941 tctatgataa cttttcctgt ttcatattcg tacccgctgt cgatttcctg ttgtgtcaaa
    15001 aatcgagcac aaggtaccga tccataaaaa gtatctgcat gcgtcgtccc catcgcagga
    15061 acatccaatc ctgcttgtgc ccagatcgtc gcccatgtcg agtgagtatg gacgattcct
    15121 ccgatttgag gaaatgaacg atataaaaca gcatgagtag gcgtatccga agagggattg
    15181 agtttacctt caatgatatg attgtccaag tccacaacga ccatgtcgtc tgctgtcatc
    15241 tcttcataac tgactccgct aggtttgata acgaaatatc gtgtttcagg atcaaaagcg
    15301 ctcacatttc cccatgtata tttgacgagt ccgtgtttag gcaagtcaag atttgcttgg
    15361 tatacttctt ctttcagttg ttccagcatt ttctatcact cctttttctt gcaattgatg
    15421 aatcgctgtt ttttcaatgt cgatcccctc acggtaacgt ctcataaata tctgatagcc
    15481 tgctatttct tctgctgaag gtgtgatggt gattccttgg tcttgtccaa acacgtcttc
    15541 tgccaagaag ttttctaaag acttcccatg tcgatccatc atataggctg ctaataacgc
    15601 gattccccac gctccacctt ctccggctgt gtccatcact gtaactggtg cctccatagc
    15661 agatgctaga atccgctgtc caacttctgg tgttttaaaa atccccccat gccccactaa
    15721 acgatcaatt tggacattct cagatttcaa tatatccatt ccgattctca ttgcaccaaa
    15781 agcactggct aaatggatcc gcatgaaatt cgctaaatca aaacggctac ctggtgtacg
    15841 tacaaataat ggccttcctt catttactcc tgtaatgttt tcaccggaaa aataaccata
    15901 agaaagtaat cccccgcaat ccgtatcgcc ttctagcgct ttgttgaata ataccgtgaa
    15961 taatttatct ctgcttatct ccatacccaa agaattggta aattcttcaa aaatactgac
    16021 ccaagcattg atatccgaag agcagttatt cgtatgaacc atgcctacta gttctcctga
    16081 aggtgtagtg accatgtcga tttccggatg gacattttct agttcttttt ctaatacgat
    16141 catagcgaag gcggaagtac cagccgaaat gttccctgtc cctttggcta cactgttcgt
    16201 agcgaccata ccggttcctg catccccttc tggcggacaa accgggattc ctggtctcaa
    16261 atttcccgta gggtcgatca gataagcccc tgattcgctt aacttgccag cttcttctcc
    16321 tgctactaga actttaggca aaatgtgatc caagtcaatc gctacgtgcg ctttttcgat
    16381 taactgacca aaaatatttt tcatttcttg atggtaggtt ttcgttgaag gatcgatagg
    16441 aaacatgcct gatgcatctc ctacgcctag tactttctca ccagtaagct gccaatggac
    16501 ataacctgca agcgttgtca aaaaattaat ttctttcaca tgttcttctt tattcaaaat
    16561 agattgatac aaatgagcga tactccatct ctgcggaata ttgtattgaa atttttccgt
    16621 taatattcgt tctgcttctt cagtgattgt atttcgccaa gtacgaaatg gtactagtaa
    16681 ctgttccttg ctatcaaatg ctagataacc gtgcatcatt gcactgaacc caatcgaccc
    16741 gattgtcgta agattctcgc catagatttc gaatacttct gctgccattt ttcggtaact
    16801 gacgcgcagt cctttccaaa tatcttctaa tgaataggtc cagataccat tttctagttt
    16861 gttttcccac tcataactac cagcggcaat aggagtaaaa gatgaatcaa tcaatacagc
    16921 tttgatccta gttgatccaa actcaatccc taaagatgta cgaccatcta tgacatctaa
    16981 catactttgc gcatttactt ccttcataat gatttaactc cctccttctc gtcaaacctg
    17041 atcaaaccgt ctttttagta gcgcttacaa tgattattgt atacttttgt acgtacatgt
    17101 caagcagtta tttacaagaa tacaataaaa aacacttgtt ttgtacgtac ataagttata
    17161 aaatgaaatt gcttacaaaa tctgatcaac cgccttctcg aaattctaga aggagagaat
    17221 acttatgaaa aaagaaaaaa aagtttcctc atcgtttatt tatttttttg gcgcatttgg
    17281 cggcattcta tttggatatg atattggtgt tatgaccggc gccttacctt ttttacagca
    17341 tgattggggt ttggcaggta aagcttcttt gataggatgg atcacgtctt cggtcatgtt
    17401 aggtgcgatc cttggcggct ctttatctgg tcttctttct gataagcttg gacgacgaaa
    17461 aatgattctt ctttctgcac ttatttttat ggctggttct gtcctttctg catctgcccc
    17521 tcacaatggt tcttattttc tgattgcagc aaggattctt ctaggtttag ctgttggcgc
    17581 tgcatctgcc ctagttcctg cttatatgtc tgaaatggct cctgctagac taaggggaag
    17641 attgtcaggg atcaatcaag tgatgattgc ttctggcatg ctcctttcct atgttgcaga
    17701 ttatttgcta aaagggttgc ctgaaacgat ggcttggcgc gtgatgttag ggctcgctgc
    17761 tgtccccgcg ctgattttat ttttcggtgt tttagctcta ccagagtcac cgcgttttct
    17821 tatgcaatct ggccgcttgg aagaggcaaa aagagtattg aactatattc ggacaccaaa
    17881 agaagctgaa caggaattcg aacaaatcca gctgaatgtc aaacaagaaa agacaaccgg
    17941 aacttcttgg catactttgt ttttagaaaa atatcgttcc ttagtcattg ccggtattgg
    18001 tgtagcagta ttccagcaat ttcaaggagc taatgctatt ttttactata ttccgctgat
    18061 cgtagaaaaa gcgactgggc atgcagcgag cgatgcattg atgtggccga tcatccaagg
    18121 aatcatttta gttgctggtt ctttgctctt tttggttatt gcagacaagt tcaatcggcg
    18181 aactttattg aaaataggcg gtagcgtgat gggactttct ttcatcctac cagctgtttt
    18241 aggaactgta ctggatgctc atacaaattc gttgttgatt ctcttgtttt tatgtattta
    18301 tgtcgcattt tattcttgca catgggctcc actgacatgg gtaatcgtag gcgaaatatt
    18361 cccactagct gtccgcgggc gagcatctgg cctagcctct tcttttaatt ggattggttc
    18421 tttcctagtt ggcttgcttt ttcctgtgat gactgcttct atgtctcaag aaatcgtttt
    18481 cggcattttt gggatcatct gctttttagg ggttttattt attcaagaga tcgtaccaga
    18541 aactcgtggg aaatctttag aggaaatcga acaaagtgca tctaaaaaaa cttatcctaa
    18601 acgtatttct atccatcatt cttaaagagg ttgtgacaga agtggtcagc ttcaagaaat
    18661 aagaaggaac cgcttagttt ccagtcacaa ggaacaattt caaaaaacag cttctcatgt
    18721 ttgatgaata ttgtgacttg tgaccagttg gtaggagaac accgtttagt ggagaggtgg
    18781 tgaaagaaac gttcagcctt gagcgatgac aaaagctctc acaaacattg cgacgagtaa
    18841 ccgtaggagc aataagaggc ggtaaaaacc cgtctcttat ttttcgtttt ctttcactgt
    18901 ttcccgttca actagtacag gttcatagag aatatttggc tgtgcctgtc ctttttcaat
    18961 cgttgtgacg atccaccgtg cggcatcttg tcccaacttt tcttttggat gcgtcaatgt
    19021 tgtcaatttt ttattcccta aatggcttaa agaagaatcg tctgttccta cgatcgatag
    19081 gtcatctgga atatgatagc cattttctgt caacttatct agcattaaac tagctatttg
    19141 atcgttatag caaatgatgc catctaaagc ttgttcttct ttcaaccgtt gtactgtttt
    19201 ttcagcgatt gtttctcgtg tttccgtggt gaaagtaatg atatcttccg tcaaaaaacg
    19261 cacattatac tgttcacacg cgctgataaa acctttcatc ctgtattttc cttgaagatc
    19321 gtcgattttt gtgatgaaca acagacgctg atgtcctttt tttatcaaat attctgctgc
    19381 cataaaaccg actttcacat catctaaaca aatataagag gcatttaact cttcataagc
    19441 agcattgatc atcaccatcg gaatgccttg ttctctcaaa gcaacataaa gtgctaagtt
    19501 tgggttatac tgattacttt tcgtaggctc aacgatcaaa ccatctaccc cataagcaat
    19561 cattttcttc aaacactctt tctccaattc gtgatcgtta ttcgtactgg ctaaaagcaa
    19621 tgaataccca gattctcgta attcattttc gattcctcgg ataatcgacg gaaaaatata
    19681 atcagagaga tatgtggtga tgacaccgat cgtttttgtt ctgttcaatg gtgttttttc
    19741 tttcttatag ctgtcatcta catatgtgcc cgaccctttt tctttacgca gatagccctc
    19801 atttaccaac acagcgattg cttgccgaat cgtatgtctg ctgacgtcat atatttcttg
    19861 cagcttgaat tctgagggaa tggttgtatt tacttcgtat tcctcacgaa ggatcttttg
    19921 ttttatctct tctgcaatat cttgatattt tgccatttgc cttgcccctt tttatttgta
    19981 cgtacatata tcataacgag tctgcaaggt ttattcaagc aaaaatcaag gtttcttgtg
    20041 ctcataaggc atacggaaca ggttcaccaa attctggaaa tccttgctca ttccacgtaa
    20101 agcattgcgc gttcgcgtgc cgattaggat tgtctaacgg atcccctttt tggttcttct
    20161 ctggtcgagc atgatagatc aaaatatcaa agcacccgtc ttctgaaacc gtaaacgagt
    20221 tgtgaccagg accaaattgc ttattttttt tagaagaaac gaatactggc tccgataatt
    20281 tatgccatga ttcttttaat aaaagattct tgtgctcatc agcccacagc aggcccatgg
    20341 catagttttc atctgttgca ctaccagaat acgtaatgaa tattttccca ttgcgagtaa
    20401 ggactgctgg accttcattt actgaaaaac cccgtttttc ccattcatac tcaggtatag
    20461 aaaggaggat ctgcttcccc tttaacgtcc aaggattatc catttcggat agataaagat
    20521 tagaattccc agtaatttca ggatcttgtt gggcccacac ataatatagc tttccttgag
    20581 aagagaaagt tgttccgtct aaactgaaag tttccctttt cgtttttatt tgtccctttt
    20641 ctatccaatc tgtattcatt ggatctatat gctgattttc aagtacgaac atgcggtgat
    20701 gatgagattc atcacgtata gttttatgat tgcttgcagc aaagtaaata taccattttc
    20761 cattgatata atggacttca ggtgcccaga tcaattcact catcggtcct tcttcatgag
    20821 cctgccatac aatcaggctt gcagcatgtt ctaattcatt cagtgacttt gcccttctta
    20881 attcaatcac ttggtagcct ggaaccgatc ctgtaaaata ataataacca tctgtatgtt
    20941 tatataccca aggatctgcc cgttgccgaa cgataggatt attataaagt gtcttcatca
    21001 taacaaattt cctcacattt tctttattct aattacattc caagagtatt tttctatccc
    21061 tacatttaat tcgtcatgtt tttctattga aaaatagttt atttcaggtt ttattttttc
    21121 tggttcttca cgtgtatttt tgtccaacag atcgctactg gttaatgtgt agtgctctcc
    21181 ttgatctagc aaattgaaat catgttcaat cgttatcttt tgttgttcat ttgtgcgatt
    21241 gatcaagaaa atagtcagct gattgccgtt atctacgatc acactgtcca cataaggaac
    21301 attttttatc ttttcacaat cataaacagg gcaaatgatt tgttgtttca atacttgacc
    21361 tttagcatat ttcgataagt gttgcatcac ataataagtt ggattcctcc atgcttgtcc
    21421 tttagcatct gtcagtatta aaggtattgt gtttactaac aatgattgac aactaatctt
    21481 tacgcgatct gcgtgtcgta aaatagccag ccctaatgaa ccaaataata atgtatcttc
    21541 catagtaaag taacaccaat cgatgggact accgacttca aaatccctat gtcttttttc
    21601 cggttgggca tgtacattcc attcatcaaa cgctagatac attgttttct cacttctcaa
    21661 atatcctttt acataatcac aagtagcaac gatttcttca atttgacgat cgaatcgata
    21721 agcttttgca aggtatgtcg ctgtatcatc acgctcattt ttaggaggtt tagtcaagtc
    21781 ttcttcatca tacttatcaa tatagttatg caaagctaaa taatctacat tctcataagc
    21841 ttcttctaat gcgatacgat cccattcagg gtacgatggt aaacgtggcg tagagcttcc
    21901 aactaagatt gtttcaattg aatcatccac taacttcatg acttttgagg cttcatttgc
    21961 taacctacca tattcataag cactcttagc ccccacttgc catggaccgt ctagctcatt
    22021 ccctaaacac cataatttaa cgccaaaagg ctttttttgc ccatttttta ttcttaaatc
    22081 actccagaag gttccttttg gaaaattaca atattctact aaattttttg cttcatcaat
    22141 tccttttgtt cctaaattca ccgtgaatat cggtttcgct tgtacatgac tgatccattt
    22201 aaaaaattcg tggagtccaa aagaatttgg ttctatacct cgccatgcta aatcaatttt
    22261 ttttggtcgt tgttctttcc tacctacagt atccacccaa tcataaccag atgtaaagtt
    22321 tcctccagga tagcgaatga cacctaattc taattccttt acataagaga ggacatcttg
    22381 tctaaacccg tcaccatcag ctgtttcatg gcccggttca taaatccctg tatagacgac
    22441 acttcccata tgctcaacaa atgagccaaa cagccgagaa ctgatctctc ctaccgtaaa
    22501 atcctctctg atttttactt tcacttcttc gtccaacagc tttccctacc tttcatttcc
    22561 tatagtcaag ataatagata caagtttcca tacgccacca gcaaagataa aaataaaagg
    22621 agggaaaaga aatatgagca gtacaaatcc taataacaat acgcaacaaa gaatatttct
    22681 caaaaaatca aaaaatactt tccatgcact ttgttcaaaa atttgtaaaa atgtgatatc
    22741 tttgttttca gaaatcacat atatccagga taaaagaaac atggaataaa taagtaaaac
    22801 tacatatcca ctaattgaaa gtacgattgt ctgttcttct aataattgat taacaagaaa
    22861 ataaataatg atcgttaaca gactaaggaa caagttctta ccaaaatgtt gctttattcc
    22921 ttgaaagaaa aaataaatga tatttcctga tccctcacct ttccattgcg ccattacata
    22981 aaaaacagca catgtagcag gcacgatagt aataacaggc aaacacacga ttacccagag
    23041 aatacctaat aagatgtaat cagaaaccac aatcaactgt ccataaagtt tagtctcaac
    23101 tattttcatc ttcctcactc tttcattcca gacatagctg ttccttgaac aaaatatttc
    23161 tgtgcaaaaa tataaaccac tagtaatggc agcattgaaa taaccgttcc agccattaca
    23221 ggtccataaa aagaaatgtt ctgccctgta aataaagcta accctgctgg taaagttctc
    23281 atttttgaac tacttgtcaa tatcaatgga taaagcaagt cattccaact attcatcagc
    23341 gtgaaaatcc ctaatgataa aagagtaggt ttgctcaatg gcaacatgat tttcaaaaaa
    23401 atattaaact cacttaatcc atcaatgcgt gctgcctctt ccaaagaatc tggtaaagta
    23461 ataaaaaagg aacgcatcat gaagatacca aatgcagttg tcatacgagg aatgatcaaa
    23521 cctgcataag tatcaagcat gccaagcaaa ttgacttcaa taaatagagg aatcatgaag
    23581 acttggaatg ggatcatcat tgtcagcaaa acgaaataga acaaaactga ttttcctttg
    23641 aaacgcatac gagcaaatgc ataccccgca agagaatcaa agaatacaga agtgatcact
    23701 gtccctccag caaaaatcac tgtatttttg atgtaatcta ataaagggat cgttgtccaa
    23761 accttaatat agttatctag ggtatagcta tctgcaaata ttctaggcgg aaatgagata
    23821 atgtcttttt cgtgtttaaa agaagaaata atcagccaca aaaaaggaaa cgcaataacc
    23881 aacaatataa ctagcatact aatcgttcga atgatttttt taccactaac tgcttttcga
    23941 ctattcacag taactgtaat cgagctttct ggtgccatac aggtttccct cctatttcat
    24001 ttgtttttct ttccgtgaca tgtacacatt agtggagata gctatagttg caacaatgat
    24061 aaacaaaacc gaagacagtg ctgatgcata gcccaattca taaggtgctg taaatcctcg
    24121 actgtaaata tattgaacag ctgtttctgt cttaaattgt gggccaccag cggttgcaac
    24181 atatacttgg tcaaacactt gcatcgaacc aatcagattc gttaaaatac aaaaacctaa
    24241 ggagggaacg atctgaggta gcgtaatgtt aaaaaactgc tgtcgatttg ttgcaccatc
    24301 catttgagca gattcataca aagagggaga gattccctga atagcagtca agagaatcgt
    24361 catagtaaca ccaaaattct tccaaacagt catgatggct accgtcggca tagcaagggt
    24421 tggatcgcga aaaaattgag ggatcggcaa gccgacttgt gaaaacaagt aaggaatcgc
    24481 ccctacattc ggatcaagca acatagaaaa aataatcgca attgacgtca atgaacaaat
    24541 aactggaatg taaaatgtcg aacgacaaaa acgattgaat ctggtatttt tagataatgc
    24601 tactgctgct aataaaccaa cgataatttg tgttggcgtt tcgagtacgg tgaaatataa
    24661 agaatgaatc aacgaattaa ctgctcgttc atcactaaat atacgtgtga aattttcaaa
    24721 acttacaaaa tttcttgatg taaagaaaat attcaaatct gtaaaactaa tagcaaatgt
    24781 tcctaaaagc ggtatgacag taaataataa taaaactaaa aaagatggag ttaaaaacaa
    24841 atacgcagca cgatcaggac gattccaata tttggtgaat ccgttcatat agtccacctc
    24901 atctttcatt ttgataaaat atacaatctt ggtttaaacg aatctgaata agatatccga
    24961 acaatcgctg tgatttttgc aataacacct atctgcaaat actaggtcga tcattcagat
    25021 atctgtatct tgccaacagt ctatcctgta taaagttctg tactattttt gcagtaattg
    25081 attgatggca tcatctgcat cgttcatgag tgtctcagga tcttctcctg ctaataattt
    25141 ttcaatcagt ggattgatga tatcgttatt gatcgcagag atttgggtaa aacctggtaa
    25201 atatggctct gcatagtcca tttgtttcga taattcagat acaataggat cattttttac
    25261 ttcttcatct tttgctactg aatccaagta ggcaggaaaa ccattttcga tactccattt
    25321 tttaccaatt tcagttgtat tccaatattt caaaaactca tagacagctt cttttttatt
    25381 attatctgta cttgccggaa tggcatatcc tacgccatct aaaatagctg tttttttgtc
    25441 tgaatcactc ccttgtggta aggtagtcac attataatca atctcgtttg ctttcaatcc
    25501 gttatttaac catggcccat tgatttcaat ggctagttga ccagcattca ttaaattgtc
    25561 catttcggcg ccagaaatcg attctggacc agtcttgtct acatggatca ttttttggat
    25621 cgcttttaac gttttaaggt tctcaggaga cgcaaattca gacttagtat aatcatcgtt
    25681 cactaattta ccgccatttg ctaacatcca gttatataac ggtgcagttc catccttatt
    25741 gaagacaaaa cctgatacat ttttactact atcagttaat tttggtgcca tttctgtcaa
    25801 ttcttcccaa gtagtaggtg gttgttcagt atctaatcca gcttgtttaa acaaatcatt
    25861 attccaatac atatacatgc cttgtacctg catggggaca aaatactgtt taccttctac
    25921 ttcacctaat tcaatagccg tttctttaaa atcagattta tctactccat catattccca
    25981 gaaatcgtct aatggttgta ccgctccgtt ttctacatac tgtgcaaaat ctggataatt
    26041 taatgcaata aagtcaggtg cttttttaga agaaatcgct gtaggaagtt tttcattcaa
    26101 attggcccaa gtcatgatat ccatttcaat tttgatgttt ttatcatttg ttttattaaa
    26161 ttcattaacg atttcttgca atatttcacc atcagatgca gtaaacccat tccaaaaagt
    26221 caaagtcact tctttatcag aatcagattc tgctgaactt ccgccaccgc aagctcctaa
    26281 aatacctgtt gcagctacca acaaagagcc aattaaccat tttttacctt tcattttttg
    26341 ttcctccttc atctgataaa cgacatctga ccacattcca agatagcggt ttcaataaac
    26401 aattgatttt cccatcttca gcttttacat ttgttgaatg acttggtttt acttgttcac
    26461 ttttaggaag attagttgct tttgtatcaa atccactcat ttcagaaaaa tcaatcactc
    26521 cctctaatga taattcagac aagtgaacag ttagattcat ttcttcttct ccacggttaa
    26581 ctgcaaatat gacaagttct ttgtcattta ttacggctat actttctaaa tacggcactt
    26641 cttcaaaatc ttccgtcgca tagtttgctg aacgaatttg tgggcttaac acctctcctt
    26701 gtccatacac agcagcttgc ataaatggat aatagatcgt ctgtctccaa gcttctccgt
    26761 ttgtttctgt cctgattggt gcaataacat ttaccaattg agcaagacaa gcaatcttta
    26821 ctctatctgc atgtttcaat aaagtgatta atagacagcc taatagtaat gcatcttcaa
    26881 aattatagtt atcttctagt aaaggaggag caatctgcca aggcgctgct tgctcatctt
    26941 gttccgctga atgataccaa atattccatt catcaaagga caggttgatc tgcttcttac
    27001 tatgtttctt cgccttgact gtatcgcaca tcgctgtcac ttcagaaatg aaccgatcca
    27061 tgtctaagga acgtgcaaga taatgagaaa gatcattttt ttggttgcca taatattgat
    27121 gcagagacaa ataatctatt tgatcgtacg cttgatcaag aactgttaac tcccaatcac
    27181 caaatgtagg catttgccga ttcgaacttc cgcataaaac cacctcaatt gaatcatcta
    27241 cgagtttcat cgccttagcc gtttcctctg ctaaccgtcc atattcttcc gctgttttat
    27301 gtccaatctg ccaaggaccg tccatttcat tccctaaaca ccatgtctta attccaaaag
    27361 ggctctcttg accattcgaa cgccttaaat cactccaata agttccttta ggaaaattac
    27421 aatattcgac taaattacga gctgcatcta ctccacgagt acccagattg acagccatat
    27481 tgatttcggc ccccacatgc tgacaccagt tataaaattc atgaatccca acttcattcg
    27541 tttcaattgt ccgccaagct aaatccaacc ttctaggtct atttgctatg ggcccaatac
    27601 tgtcttccca acgaaatcct gacacaaaat ttccaccagg atatctaatc atgggaatgt
    27661 tcaattcttt taccaagtga atgacgtctt ggcgaaatcc acttttatta gctgatggat
    27721 gatctggctg ataaatccct tcatatactg ctcgtcccat atgttcaata aatgaactgt
    27781 acaaccgcgg atcaactttg cctacacgct tgacactact aatcaacatt gtggcttcca
    27841 taactgacct cctttaattt atacatgaat aatacacctc gttgaaagcg ttgtcaataa
    27901 tataaatgtt aaacttactt attttttatc gattttaatt aaccaaaata ttaataatga
    27961 ttaaaacaaa taatttaata taacttttat taacaaatat gttaatatat aagaaaaacc
    28021 ttttaagagg tgatatatat gcaactacaa ataaacgaag aatccttacc ggtatatgaa
    28081 gcattagcta gtaaaacaag gatcaagatc atccagctac tttccaaaaa aaagatgaat
    28141 gtcaaagatt tggcaaaaga acttggagtg agcagtgcta tcacgacgat gcacgtgaaa
    28201 aagctagagg aagcaaacat catcaaaaca gaaaaagtag gacaacaaaa aatttcgagc
    28261 cttcgtgtcg ataaaatcga tatcagtttt ccagaaaaaa tattcaatgc ttttgatacg
    28321 aaagaaacat cgatacctat tggtcactat acaaattatg caattgaacc aacatgcggt
    28381 ttagctacga tccatgattt tatcgggaaa gtggatgaac cacgctattt tatggatcct
    28441 cgaaggatgg atgcccgcat tctctggttt accagcggat ttgtcgagta tcaggcacct
    28501 aattttttaa atacagagga tacattggag atgttggaag tttctgtgga aatttcttct
    28561 gaatttcctt tttcaaatga caattggcct tcagatatca ctttcagcct aaatggtgtg
    28621 gagctaggaa catggacgag ccctggagat tttgctgata tccgcggtaa atatacgcct
    28681 gattggtatc cagataatct taatcaatac ggcttgctga agacgatccg aattaccaaa
    28741 cacttgacga atatgaatgg agaacccttg tcaaacatca cgatcaacga catccctaaa
    28801 gagcaagaca cttggcattt gcgaatcgaa gtgaaagacg atgccaaaca tgtggggggc
    28861 tgtactttat ttggtaaagg attcggtaat tacgatcagg atatcaaatt aaaagtttac
    28921 tacagctgat atttttcttt tctattttat aaaacaaaaa cagccaatcc ctatgaaggg
    28981 acggctgttt ttgctttatt gttatttcca gaaatcatcc aaaacggaaa ttggcaaatg
    29041 tcgtttatgt tgtgtcttgt tccaccatgc ttcgattttt tcttgtgctt gagcgggaac
    29101 ttcttttcct tctagataat catcaatttc attatatgtg acacctagtg cgacttcatc
    29161 tgcaatcatt ggtttcccat cttcaagatc tgcagtcgga atttttgtgt ataatttttc
    29221 cggtgcattc aatgcttgaa gcaattgttt cccttgtcgt ttgtccaaac ggaataatgg
    29281 caaaatgtcg gctccaccat caccaaattt cgtgaagaat ccagtaatgt tttctgcagc
    29341 atgatctgtc cctaatacgg ctcctgctct ttctccagct acggcatatt gcgtaatcat
    29401 acgttgacgt gctttgatat ttcctttgtt aaaatcgctg attcccacac ctgcttcgct
    29461 taatacttgt acttgtgcat caacagctgg tttgatattt actcgcagac taacatcggg
    29521 ctggataaag tcaagagcag cttttgcatc ttcttcatct gcttgttcac cataaggaag
    29581 acgaacagcg ataaattgat agttttcatc ttttgtttct tcacgcattt cttccatcgt
    29641 tagttgcgcc aaacggcctg ctaaagtaga atcttggccg ccgctgatac ctagtacaaa
    29701 agttttaaga aacggatatt ttttcaaata tgctttcata aagtcaatac ttttacgtat
    29761 ttcttctttt ggatcaattg ttggtttgac acctaattca ttgataattt ctgtctgcag
    29821 gctcatttat tctcctccta tgcttctgta tgaagttctg ctacgctttt tttcattcgt
    29881 tccatgatag ccattttatg gttccagcat tcagtagaca gatcgactgg gtatttttgc
    29941 ggattcaagt ctcgtttata ctcttcccat agtgaatcta gattttcttt tgtatattct
    30001 ttgatttcgt ttagtgcagg taattcgtaa actctttttc cttcgataaa gatatcttgt
    30061 aaaactgggc gagctgtaaa gtctcgaaca gttttattga taaacgtatg cactggatgg
    30121 aacatgaaga tagcttcttc ttctcgaggg tcttcatccc ataatgtcac gtagtctcct
    30181 tcagatttcc catcaaaatt tcgagtgatg cgccacactt gttttttccc tggagtagtt
    30241 actttttctg cattgcttga taacttgatc gtatccatca tttttccttc atcatcttcg
    30301 atcgatacta gcttgaatac tgcaccaagg gctggctgat cataggctgt gatcaatttt
    30361 gtcccaacac cccatacatc aattttagct ttttgcatct ttaaattcaa aatcgttgct
    30421 tcatccaagt cattcgaagc atagattttt gcttccgtaa atccggctgc atctaattgt
    30481 tcccgaacac gcttagatat ataggccata tctccactgt cgatgcgtac gccttggaag
    30541 ttgattttgt cacctaattc tttcgccacg cgaatcgcac ttggcacacc tgatttcaac
    30601 gtatcataag tgtcgactaa gaacacgcag tctttatggg ttttagcgta ggccataaat
    30661 gcttcataat catttccata agattggact aacgaatgag catgggtccc gctagctggt
    30721 atcccaaata tcttcccagc acgtacattg cttgttgcat ctgctccgcc gatatatgcc
    30781 gctctagttc cccagactgc tgcatccagt tcttgtgccc gtctagttcc aaattccaat
    30841 agtggatctt ctccgatgac agatttgata cgagctgctt ttgtcgcgat caacgtttgg
    30901 aaattgacca tattcaacag tgctgtttct actaattggc agtgcgctaa aggcccttcc
    30961 acttggatta atggttcatt tgcaaaaact agttctcctt cacgagcaga acgaactgtt
    31021 gctttaaatt caaaattttg taaatactcc aaaaaacctt caggatagac ttctaatcca
    31081 cgcaagtaag caatatcgga atcattgaac gtcagattct ctaaataatt gactaaccgt
    31141 tccaatcctg caaaaaccgc gtatccgtgg ttaaaaggca tttctcgaaa gtaacattca
    31201 aaaacagcat gtaaatcagc tcgacctaat tcccagtatg tctgcatcat attgatttga
    31261 tatagatccg tatgcagcgt caaactgtcg tctgggtaag tattattcat cttgtattac
    31321 tcctcttggc aagttttcat ttcctaccca ttataacaaa aaaaattaat aggaaaagaa
    31381 atcttccatt ttttctgaaa aagcacatgt tttacggata tttcttgcta aaaatgaact
    31441 ccatctgcta aaactatcct ttcttattgg tatttaacct aaacgagaaa gaatacttat
    31501 tttattcaat gtatccgaga cacgaagaaa ttagcgtttt ctatttttct tacttgataa
    31561 aataagtaga ttttcctact acaatcgtag ccgttaagat accgatgatt gaacaaatat
    31621 aaccaaatgt ctctacaaca taatcaatgg caaaagtttt tgaagcaata atacgtcttt
    31681 ccttttttgc ttcttatccc caaagaaacg gatataactt attatgtatt gaattttttt
    31741 ggaaaggcga attatgacaa gatatacctt gcgttgaaaa aactcgcgga aaaaatatca
    31801 cacgttttta aaaagataca aagtaagaaa gcagctgtca atagaatgga aacaaaaatg
    31861 gcgagacatc aatcagtctc gccttgtttt ttattgtata cattagatat ggagcaggtt
    31921 accataaaga tgattattag tcgttattaa atagaatcct tcgtattttt ttgttctatc
    31981 actacaatat ctttatttgg acgtttgaat tgtattccgg cacgttctag ctcctgtttc
    32041 actcctacat ggactttgac atcttctcgt gtcattttta aaacgtgatg aatcgcatag
    32101 tcgatttctt gccttgtgat ttttttcttc gtattgatca tcacgacttg ctttttatca
    32161 tatgtgtcta ccaatagcag ttgtgttcga tctaacgaaa ataatttgta ataattaata
    32221 gccgtattcc caaaaaagaa ttccactact ttaggataaa atgtcttaat atttaaatta
    32281 ctattcacag gtgtttcgat tagcttcatc tcattcctct tttctatata gtcgatttag
    32341 atccaatctt aagttatgtt accatctttc ctagcattaa ggaatcggtt acaattgaac
    32401 ctttaaagac aaaaggattt accttttatc acttaatagt ataacataat ttcgtgagaa
    32461 aaaaatacaa ttttttcttg accatctcgt tacgtaaagg attatgctta catttgtcag
    32521 gaggaaaaat aatggagtac acaataaaga aaatgtcaga gatatcagga gtcagtccga
    32581 gaaccttgcg cttttatgat gagattggac tgctaaaacc agctaggatc aattcttcag
    32641 gttatcggat ttatggcaaa aaagaagtcg atcgtctgca acacatctta ttttatcgaa
    32701 caatggcatt caagttggat gatatccaag aggtcttaga caatccatct tttgaccacc
    32761 agaaagcttt aatcaagcat cagcaaatgt tgctggaaaa acgggcacaa atcgataccc
    32821 tcttgacaac tgtccaacag acacttgata tgtacgaagg aggaagaaaa atgagtgaca
    32881 aagaaaaatt tgaagggttt aaacaacaaa agttgaaaga aaatgaagaa tcctatggac
    32941 aagagattcg acaaaaatac ggaaaaaaaa aggttacagc atccaatgaa aaatggatga
    33001 atatggataa agaaacgtat gaagaaatgc aaactgttga aaagcagatg ttatttgatc
    33061 tcactcttta cttaaaagaa ccagaagatg aaagcttagc agaccgtatc ttccaagctc
    33121 ataagaagtg gttctcttac agctggcctg aatatcagcc aaaagcgcat aaaggactag
    33181 gaatgatgta cgaagccgat gaacggttca ctgcatatta tgacgagcga agtggtcatg
    33241 gtgcagcaaa agctttaaat gaaattatcc aaaaaaaggc gtaatctttt acatgatcca
    33301 acaaaaaagt acacgtaaaa tgacctaata acaaaacagc agacacccaa gttagatttt
    33361 tatgctaact tttggtgtct gctccattct ttcacaagtt ttttcatcct actttaaata
    33421 tattcatcgt tgaataaacg tataaggtat tttcacttta tccttttttt ctgtaagaaa
    33481 caattgaaat gccatttctc cgcaagcttc cagatggtgg tcgatggttg ggaaaccaag
    33541 aagctcgctg atcagcaaat tatcgcgtcc aatcaccact ggtgtcttat cttgcagata
    33601 gctttgcaag atgcctgctg caaccatatc cgaatttgta aagacaccat caactccaag
    33661 ctctttaaaa aagaagcctg cttgttttcc tttctctgca tctatacaat cccaaaaaac
    33721 aagtgattca tcaaaagatg gaaaaagttg atgacatagc tgcaaagtga tttttgagtt
    33781 ataacttagc ctgccacttc gccccaacgt gatgcccaat tgctttatac cttgctgctt
    33841 caaataagta agaccttctg taattgattg ttctcgatca ataaagacac agcttgcctg
    33901 ttttcccttg atttcttcac aaaaaactag aggtccatat ttttgatatg ccaataaatc
    33961 atctagtgta ttggcacgag acgtaatgat caatccatcg aaagctttag ctgcaaattc
    34021 ttcaagatac cttcgctctt tttctttatg atagcccgtc ggtaaaagtg tcactttgta
    34081 atcataatca aacgctgctt caatgatccc actgatcaac tgatcaaaat acggatgatt
    34141 ggtataaggc agaatcactc caatgctttt cgtttttcca taacttaact gtctggcatt
    34201 tgcattaggg atataatcga cttctttcat gaccgcaagt atttctttcc ttttttcctc
    34261 atctacatat ggatgtccat tgattacacg tgaaatagta gaaactgaat aacctgtgag
    34321 ctttgcgata tctcgaatgt ttgccactgc tgcttcgcca cctatacaaa aagattttct
    34381 tgtaaatagt ctaccacagc tggaccgcat tcaaaaaact gatacatttt tccttcatgc
    34441 gaaaaactga tccgctgccc gccccaatat atatcttttg tcaaattttc gatttcattc
    34501 attggtattt cttttactat attcctattc aacccttgaa aactgtacaa cgttaatacg
    34561 gatcccttga actcgacaaa cgcataaaaa taattgttcg ttgtgccatc tgttatctct
    34621 acataagtat tcattgcaag ccctcctttt gatactttga gtatagtgta tgaaacgcgt
    34681 ctcatagcaa ggagtttgtt ttgccaaaga aaagttttat tcctctttca ttattaccaa
    34741 agtctcttat tttaaattga atacattata atgatactgt aaatatatct ttattattag
    34801 tgaatg
//
LOCUS       DAZAID010000019        33413 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000019 DAZAID010000000
VERSION     DAZAID010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.019"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..1068
                     /locus_tag="ACG9BE_000884"
     CDS             157..1068
                     /locus_tag="ACG9BE_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN3031656.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1446..2363)
                     /gene="rnhC"
                     /locus_tag="ACG9BE_000885"
     CDS             complement(1446..2363)
                     /gene="rnhC"
                     /locus_tag="ACG9BE_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFN3031657.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            2565..3002
                     /gene="zapA"
                     /locus_tag="ACG9BE_000886"
     CDS             2565..3002
                     /gene="zapA"
                     /locus_tag="ACG9BE_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFN3031658.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            3060..3608
                     /locus_tag="ACG9BE_000887"
     CDS             3060..3608
                     /locus_tag="ACG9BE_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFN3031659.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            3723..6083
                     /locus_tag="ACG9BE_000888"
     CDS             3723..6083
                     /locus_tag="ACG9BE_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFN3031660.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            6170..6484
                     /gene="trxA"
                     /locus_tag="ACG9BE_000889"
     CDS             6170..6484
                     /gene="trxA"
                     /locus_tag="ACG9BE_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFN3031661.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            6666..8444
                     /gene="uvrC"
                     /locus_tag="ACG9BE_000890"
     CDS             6666..8444
                     /gene="uvrC"
                     /locus_tag="ACG9BE_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFN3031662.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            8589..9488
                     /locus_tag="ACG9BE_000891"
     CDS             8589..9488
                     /locus_tag="ACG9BE_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3031663.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            9481..10743
                     /locus_tag="ACG9BE_000892"
     CDS             9481..10743
                     /locus_tag="ACG9BE_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3031664.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            10904..12802
                     /locus_tag="ACG9BE_000893"
     CDS             10904..12802
                     /locus_tag="ACG9BE_000893"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFN3031665.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(13132..13851)
                     /locus_tag="ACG9BE_000894"
     CDS             complement(13132..13851)
                     /locus_tag="ACG9BE_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFN3031666.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    14041..14088
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            14188..14520
                     /locus_tag="ACG9BE_000895"
     CDS             14188..14520
                     /locus_tag="ACG9BE_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031667.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            14507..16510
                     /locus_tag="ACG9BE_000896"
     CDS             14507..16510
                     /locus_tag="ACG9BE_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFN3031668.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            16529..16999
                     /locus_tag="ACG9BE_000897"
     CDS             16529..16999
                     /locus_tag="ACG9BE_000897"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFN3031669.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            17020..17604
                     /locus_tag="ACG9BE_000898"
     CDS             17020..17604
                     /locus_tag="ACG9BE_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFN3031670.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            17619..18605
                     /locus_tag="ACG9BE_000899"
     CDS             17619..18605
                     /locus_tag="ACG9BE_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFN3031671.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            18595..18906
                     /locus_tag="ACG9BE_000900"
     CDS             18595..18906
                     /locus_tag="ACG9BE_000900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFN3031672.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            18928..20709
                     /locus_tag="ACG9BE_000901"
     CDS             18928..20709
                     /locus_tag="ACG9BE_000901"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFN3031673.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            20702..22078
                     /locus_tag="ACG9BE_000902"
     CDS             20702..22078
                     /locus_tag="ACG9BE_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFN3031674.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            22085..22720
                     /locus_tag="ACG9BE_000903"
     CDS             22085..22720
                     /locus_tag="ACG9BE_000903"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFN3031675.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            22750..24105
                     /locus_tag="ACG9BE_000904"
     CDS             22750..24105
                     /locus_tag="ACG9BE_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFN3031676.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(24297..25691)
                     /locus_tag="ACG9BE_000905"
     CDS             complement(24297..25691)
                     /locus_tag="ACG9BE_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFN3031677.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            25783..26754
                     /locus_tag="ACG9BE_000906"
     CDS             25783..26754
                     /locus_tag="ACG9BE_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFN3031678.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            26751..27533
                     /locus_tag="ACG9BE_000907"
     CDS             26751..27533
                     /locus_tag="ACG9BE_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFN3031679.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(27702..28073)
                     /locus_tag="ACG9BE_000908"
     CDS             complement(27702..28073)
                     /locus_tag="ACG9BE_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3031680.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            28207..28650
                     /locus_tag="ACG9BE_000909"
     CDS             28207..28650
                     /locus_tag="ACG9BE_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFN3031681.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(28693..29502)
                     /gene="yidA"
                     /locus_tag="ACG9BE_000910"
     CDS             complement(28693..29502)
                     /gene="yidA"
                     /locus_tag="ACG9BE_000910"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFN3031682.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(29521..30888)
                     /locus_tag="ACG9BE_000911"
     CDS             complement(29521..30888)
                     /locus_tag="ACG9BE_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN3031683.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(30872..31702)
                     /locus_tag="ACG9BE_000912"
     CDS             complement(30872..31702)
                     /locus_tag="ACG9BE_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFN3031684.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            31865..32302
                     /locus_tag="ACG9BE_000913"
     CDS             31865..32302
                     /locus_tag="ACG9BE_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFN3031685.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            32378..32998
                     /gene="rpoE"
                     /locus_tag="ACG9BE_000914"
     CDS             32378..32998
                     /gene="rpoE"
                     /locus_tag="ACG9BE_000914"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFN3031686.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(33161..>33413)
                     /locus_tag="ACG9BE_000915"
     CDS             complement(33161..>33413)
                     /locus_tag="ACG9BE_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3031687.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT        10852 a         5726 c         7186 g         9649 t
ORIGIN      
        1 tcaaaaggag atttcaggat atgccaaaaa tgaaaaaaat cattttagca gtaatgttat
       61 tttctacggt tatatctttt acagcccctt ttttgcttac ttaagctgat tttaagtttt
      121 ggcccttagt ctggatctac gaggtgagag tcaagaatga aaaaaataat tggagctttt
      181 gtatttgcaa tcattatcat tattatctgt tttcaaatag atgtttttgg tcaaaaaaag
      241 atagttggac aaacggtcag cagtacgaac agtacggtta caaagacaaa ggaatcattt
      301 gatgatacag aacaagagac attaatcaaa atcggaaaaa atgaccgtac actatataca
      361 gatgactcgc taactacccc acttgcaact ataaacggcg gagagctggc ggagtttctt
      421 tctgaaacaa aaaatgctta tcagatcaag acaaatgatg gctatactgg atatttagcc
      481 ctcgaagatg gaaccaaagt cacaaagaat atacaaacaa aaccggaaga gctatctgat
      541 gcagtgattg tattagatcc tgggcatggc ggaaatgata ctggggcatt aagcaatgat
      601 gaacaaacag aagaaaaaga tataacatta agtacagcca taaaagtaaa aaaggcgcta
      661 gaggatgctg gagcaacggt ttatttgacc catactacag atgaattagt acagcttgga
      721 gatatatgcg actacagtga agaaaaaaag gcagatgtct ttatcagttt acacgcagac
      781 tctacggaat atgcgaatga agcaactgga atcacgacat attattacta tggacaagag
      841 gaaacattag cacagacaat tgctgactcc ttcactgatc tgcctctgga ttcaagagga
      901 atctccactg ggaactatca agttcttcga gaaaatcttc aaccttctat tttaatcgag
      961 atgggttata tgaataatga tagtgattta aaggaattag taacagacag ttatcagcag
     1021 cagattgctg caagtttgac acaagcattg accacttatt ttcagtaata aataagccca
     1081 gccaaacaaa aatagctcct catatttttc gtttggccgg gcttattatc gcagatgcaa
     1141 gcttttaaac accgtttaag gaagaggtcg tgagcctgcc gttcaactct gagtcacaag
     1201 gagcaatttc aaaaaacagc ttctcatgtt tgatgactat tgtgacttgt gaccgaggag
     1261 ttggcatgag aacaccgttt aagaaaaagg ttgtgaaatc aacgttttgt tctgagtatt
     1321 agagaaacaa acggaaaaat agttcctcat attttgccgt ttgttgagct aataccgagg
     1381 atcagatgat tgaacaccgt ttattcggaa caagggactg tgacatgact tttgtcacag
     1441 ttccattatt tttttagtag cttcaaagct ttttctgtat tggcaaaatg aagtttggca
     1501 tatttttcaa gcataggcat gccgcctttc tccaatagtt ttgccgctac ttggtcagat
     1561 ttcgttccag ctcctgacgg aagggtgatt ccagcttcag ctgatgcttt gtctaactct
     1621 tccaaaaagc tggcacgact gatgatagaa gcagcagcaa cagctaaatg atattgttct
     1681 cctttagtaa cgaagtacag tttttcttgt aattgatttt tttctaaacg cgcatatttt
     1741 ttaaaatttg cttccggcgt aaactgatcg atcaaaatcg cttctggttt ttctggctcg
     1801 atttttttta gcagcaaatg gatcgcttga ttgtgcaaag cgactttcat ccgaacggca
     1861 ttgtattttg gttggatctc attgtatttt tctggagata agacaagcag tttatagggg
     1921 atggtttttt ttaagatgtc agcaagctgg atgatttggc tatcttttaa ctcttttgaa
     1981 tctctaacac caagtttacg taattctgca agctgatcac gtttcacata agcagcacat
     2041 actgttagcg gaccgaaata gctgccattt ccgacctcat cactacctaa aacggataat
     2101 tgactgatat tttttggcaa agaagaggct gtttttgctg gcttggcttg tttatcctgt
     2161 gcctcccccc atttatcagc ttctattgct gcattcgtac cttgaaacat cacttttccg
     2221 gatgtataag cggtaatggt cgtcccgtct ttttttgcag caaagctagt ataaggaatc
     2281 tgtttattta acaaataaga gcgatagtat gctttcattt gactaagttg gtcgtttgtt
     2341 gctttgataa tgacgttgct cataaaattc tcctcgtttt tatacataaa actataccgc
     2401 aactcacgac ttttcgtttt ctaagagatt gcgagacata gcattttttt aaagtttatc
     2461 atataatagt taagtatgtg aaaaatattt gtggagaacg gtgaaagaac caacttcatg
     2521 gtacactaaa taaaactttc atcaaatagg aggaaatgat tcgtatggcg cacgaaaaaa
     2581 cacgctacaa agcggtgatt gccaaccaaa catatactat tatcggacgg gaaaccaagc
     2641 atcatatgga tatcgtaacg aagctgatca atgaacagtt agcagaattg aaacaattat
     2701 caccacaaat ggataatgaa caagcagcaa tcttgatggc tgtcaatgcg ctttcggatc
     2761 aattgaagaa acaagaaaga atattggagc tggaagaaga aacagcagaa ttaaagaaaa
     2821 aaatgatcaa atttacagag cttgaaaatc gtgtgaaacg cattgaagca atcgagaacg
     2881 aagctcggga agtattgaaa gaaaatggtc aagcagatca taccattaaa aatcatgtgg
     2941 aagcacaaca gattttaaac gagaaacgaa aaggacaaat taaacaaaaa tcttctaatt
     3001 aagatccttt tatcaaaata agaaaaattt aaaaaagtta gaagagaaag gaacgtagca
     3061 tgctgagttt gctgatttta tttttattgc taattgcgtt tttttctgga gcacgtcgcg
     3121 ggtttgcttt gcaaatcata ttcgcgattg gatatgtatt gtcatttatt gctgcccagc
     3181 atttttacaa accattagca agtcatttgg aattatacat tccataccca gctgtcaccc
     3241 caacatcaaa gttagcattt tttgatcagg tatttgcctt tcacctagat gaagcttttt
     3301 atgcaggaac tgcttttctg attattttgc taattggctg gctgcttacc cgatttgtgg
     3361 gggtttttgt ccatggattg acgtatgtgc cgatcttgag acaggtagac tggattgcag
     3421 gaggcatcct gtcactaatc atggcttacg ttactatatt tttgattttg cagttacttg
     3481 cctttgttcc attagatgtg gtccaaaatc aattcggcga gcatagtttc gctcgtttta
     3541 tcgttgagag aacgccattt cttaccaaca agatgtatga gctttgggta acgaatattt
     3601 tgaattaagg aaaatagtgc tggaaaatat ttcgtttttt gtacagagta gtttatttaa
     3661 gaaggctgcg tagcgtatgt ccggcacagc tcttttttca tagtgaaaga taaggtgata
     3721 aaatgaataa ccgaatcctt gaaacattgg aatttgaaaa agtcaaacag atggtcagac
     3781 aatttgtagt gactgcacaa ggaaaagaag aactggcaga attggtacca gtgagtgaga
     3841 aacaaacgat cacaaactgg cttcaagaaa ctgaggatgg attgaaagtg caacgtctgc
     3901 gaggcgggat ccctatacct aaaattgaaa atatccgtcc ccatatgaaa cggattgaga
     3961 tcggagcaga cttgaatgga gtggagcttg cacaagtttc cagagtattg tctacgacaa
     4021 gcgaattgaa tcgtttcatt gatgatcttt ctgacagtga aatcgaattt gcacgtttgt
     4081 atatgtgggc agatcagtta gtcacgatcc ctgttttgag ccgccgttta aaggaagcaa
     4141 tcgatgaaga cggacgcgtg actgatgacg cttctcctga gttaaagagc ataagacaga
     4201 acattcggcg aagtgaacaa gctgttcgtg aacagttaga tgggatcgtc cgtgggaaaa
     4261 atgcaaagta tttaagtgat gcgatcatca ccatgcgaaa tgaccgctac gttatcccag
     4321 tcaaacaaga ataccgcggt gtatttggcg gtgtagtcca cgatcaaagt gcttcaggtc
     4381 agactttatt tattgaacca aaacaagtgg tggatttgaa taatcgtttg cgtcaatacc
     4441 aaatcgctga acgaaatgag attcaacgta ttttaagtga actttctgct gaacttgttc
     4501 cccatcgtca agaaatcatc cacaatactt atgtgattgg taaaatggat ctgatgaatg
     4561 caaaagcacg atttggtaaa gaagtaaaag ccatcgtgcc gggaatcagt gaagacaatc
     4621 atgtcgtctt gaaacaagca agacatccac tgatcgatca ggaaaaagta gtttctaatg
     4681 acatcacaat cggaaaagac tatcaagcca tcgtcattac aggacctaac acaggtggga
     4741 aaacgatcac gttgaagact ttaggtctgt tacaactgat ggggcaagca ggattaccga
     4801 ttcctgcagg tgaagaaagt caaatcggta tttttgagga agtatttgcg gatataggag
     4861 acgaacagtc gattgagcaa agtctatcga ccttttcttc tcatatgacg aacactgtag
     4921 atattttatc aaaagtcaat gaaaaaagtc tggtattatt tgacgagctc ggtgcaggaa
     4981 cagatccgca agaaggtgct gctctagcaa tcgcaatctt agatgatctt ggtaaaaagt
     5041 ctgcttatgt gatggcaaca acgcattatc ctgaattgaa agtttatgga tataatcggg
     5101 caaatacgat taatgccagc atggagtttg atgtggatac attaagtcca acgtatcgtt
     5161 tgctgattgg cgttcctgga cgaagcaatg cgtttgaaat ctctagccga ttaggtcttg
     5221 atacagaagt tattgatgaa gcgaagcagt tgatgaatga tgagagccaa gatttaaacg
     5281 aaatgatcac agatttagag aatcgccgga aaatggcaga gaccgaatat ctggaaatgc
     5341 gtcattttgt ttctgaggca caagaacttc acgatgattt gaaagaagct tacagctatt
     5401 tctttgaaga acgtgaaaaa gaaatggaaa aagcaaagaa aaaagcaaat gaagtcgtgt
     5461 cagaggcgga agaaaaagca gaaaaaatca ttgctgatat tcgtaaaatg caacagcaga
     5521 ttgggcaagg aaatgtgaaa gagcatcagt tgatcgatgc aaagacccaa ttagcaaatc
     5581 tacatcaaga agaaactttg aagaaaaata aagtattgaa gaaggcgaaa gaacaaaaaa
     5641 cgttgaaacc gggagacgaa gtgttggtta cgacttacgg ccaaagaggg acgcttttac
     5701 gtaaaaacgg gaatcagtgg caagtagaaa tcggtatatt aaaaatgaat gtgtccgaag
     5761 acgagctgac gcctgttgct cctcaaaaag agccgacaca acgagttatc catgctgtac
     5821 gatctgaatc tagtagtcac gtgccgaatc aattggacct tcgaggaaaa cgatacgaag
     5881 aagcgctaag tgaggtagat caatatttgg attctgccat tctagctggt tatcctcaag
     5941 tgactattgt ccacggaaaa ggaacaggcg cacttcgcaa gggaattact gattatctta
     6001 aaaaccatcg gagcgtcaaa agctttgagt ttgcaccggc aaaccaaggc ggaaatggtg
     6061 cgacaattgt taaatttaag taaagcttac taaaggtaag cagatggttc gctatccaag
     6121 tatagaaatg ctatactagt catagatttt aaaaatggag gtctttgata tgtcacaagt
     6181 aattacagat aagacattta atgaagaaac agataaagga cttgtattga ttgatttttg
     6241 ggcaacttgg tgtggtccat gtcgtatgca agcaccgatt ttggatcagt tggaacaaga
     6301 atatgatgaa gaagaattca gaattgctaa aatggatgtg gatgaaaatc cagaaactcc
     6361 tcaacaattc ggtatcatga gcattccaac attaatgttg aaaaaagatg gtcaagtagt
     6421 agaaaaagct gttggtgttc attcaaaaga acaactacgt caaatgatcg atcaatattt
     6481 ataaaaactt acagcaaaaa taagctgagg gtgggactga atccagttcc gtcctcggtt
     6541 tttttgcggt tgagttaagg ttattcaaat taatattttt tgagtataat tacgttgtca
     6601 ggctggattg tttttttgac agacatgcta taataacttt aattgaatat gaaggggagt
     6661 accagatgaa cgaacgaatc aaaaacaaat tagctttact tcctgatcag cccggctgtt
     6721 acttgatgaa agataaaaat ggaacgatca tttatgtcgg caaagccaaa atattaaaaa
     6781 atcgtgtccg ctcatatttt actggtagcc ataatacgaa aacggaacgg cttgttagtg
     6841 aaatcgaaga ctttgaatac atcgttacag aatcgaatat cgaagcattg ctcttagaga
     6901 tcaatctgat aaagaaaaat gatcctaaat acaacattat gttaaaggat gataaaacct
     6961 atcctttcct taagataacg aatgaaaaat atccccgttt ggtgattact cgtaaggtac
     7021 taaaagacaa agcactgtat tttggaccat atcccgatgt cggtgcggcg aatgagacaa
     7081 agaagatcct cgatcgatta tttcctctga gaaaatgcaa acctagtcag acgaaggagc
     7141 cttgtcttta ctatcatttg ggacaatgcc tgtgtcctta ttatttcgac gtagatccga
     7201 aagtctatac gaaaatcgtt gatgaagtga aaaacttttt aaacggagga cacgaaaaaa
     7261 tcgagtcaga gatccatcag aaaatggaaa aagctgctga aaacatggag tttgaaaaag
     7321 ctgcagaata ccgagaccag atccgtgcca ttgagacaat catgactcgc caaaaaatga
     7381 caaatactga tttactggat cgagatgtat ttggttatgc gattgataaa ggatggatgt
     7441 gtgtccaagt gtttttcgta agacaaggaa agctaattga acgagatgtt tcaatgtttc
     7501 ctttctataa cgaagcagaa gaagatttct tgacttacct cggtcagttt tatcaggaaa
     7561 atgagcattt cattcctaaa gaagtcttga ttcctgatac gatcgacaag cccagtgtag
     7621 aagctttgtt agctacgaaa gtgctgcaac caaaacgagg agaaaagaaa aaattagttg
     7681 aattggcgag taagaatgcc tcagttgctt tgaatgaacg gtttgatctg attgccagaa
     7741 aacaggaacg gacgatcgga gcagttgaac gtttaggaaa cgcgatgaat atcccgaccc
     7801 caatacgtat cgagtctttt gataactcta atatcatggg gacgaatcct gtggctgcga
     7861 tggtcgtatt cattgatgga aaaccggcaa gaaaagaata tcggaaatat aaaataaaaa
     7921 cagtcaacgg accggatgat tatgcatcaa tgagagaagt catctatcgc agatattcgc
     7981 gagtgattcg cgaagggttg cctttacctg atttgatctt gatcgatgga ggaaaaggac
     8041 aagtagatgt ggcaaaagat gtcttagaaa atcaattagg aatcgatatt cctattgctg
     8101 gcatggtaaa aaacgataaa cataaaacaa atgagttgct attcggtaca gatttggctg
     8161 tagtaccgct tgagcgaaat tcaccagagt ttttcttact ccagcgaatc caagatgaag
     8221 tccatcgatt cgcaatcact ttccatcggc agctacgcag taaaaacagt tttgcttccc
     8281 gattagatga aattgaaggg ttaggaccaa aacgaaaaaa aatgctgctt aaagagttca
     8341 aatcgttgaa aaatatcaca gcagccacga cagaagaatt acaagaaatc ggactgccga
     8401 aaaatgtcgc acaaaacgta tttgataaat tacatcagga atgaggaaag ggcactttac
     8461 aaagaaagta aagtgtccta tcattttatg tacaacaaaa agagcgttta cgttttgttg
     8521 gtaaataata aatcgcttac gtctggtgtc taaggatata tgtgatttct tgaagaagga
     8581 gtgaagaaat gttagaagtc aaggatcttg ttaaaacttt tgggaactac acagctgtcg
     8641 atcatgtctc tttccaaatc cccgatggaa aaatcatggg gctaattggc caaaacgggg
     8701 ctgggaagac aacgactttt cgtttgatct tggatttttt gacacaagac caaggagaag
     8761 tgttatggaa tggccatcat cttggggaga aagattatga tgtcatcggt tatcttccgg
     8821 aggaacgggg actttatccg aaagtaacga ttcaagacca actgctttat tttgcagaac
     8881 ttagagggaa atcaagaaaa gagattgaac caaaaatcga cttttggatg gaaaaatttc
     8941 aggtaaaagg gaaaaagacg gataaagtaa aatctttgtc taaaggaaat cagcaaaaag
     9001 tccagctgat cgccacgtta atccatgaac ctaaattgat cattttggat gagccattca
     9061 gcgggttaga tccggtaaat gctgaattgc taaaagatgg aatattggaa ttgaaaaaac
     9121 atggttcatg tgtcattttt tctagccaca atatggataa tgtagaaaaa atctgcgatc
     9181 atttgatcat gttgcgaaac ggaaaaatgg ttttgaatgg aaaagtacat gagattcgtg
     9241 aatcatttgg ccgtacgaag ttattcttgg aatctggttt aagccaacag gaagtagaag
     9301 aaatcgaagg cgtgaaaaaa gtgatccttc atgaagacaa tacgttagag attaccttag
     9361 aagatcctga agctgggaaa gaaatcttta ctcgtgcgac tcagttcggc tacattccta
     9421 tgtttaacca gcaaccgcca actttggaag agattttcaa attgaaagca ggtgaaatcg
     9481 atgcgtaagt tttggattat taccaaagat gtctatttaa aaaatgttaa gtcgatttct
     9541 ttcttgatca tgatattagt cccttttatc atgctgggta tcatttatgt agcaggaaac
     9601 tttgctcaac aaaatagtga tgtcaataaa atcggcgtat tggctgataa tgagcaaatc
     9661 agtcagcaaa tgagccaagt caaaacagat gattttgagt tcaaagcagt tgattctgaa
     9721 gaaaaagcac aaaaagagct ttcggaagaa aaaatcgatg cgtatttagt agtctcaact
     9781 gagaatgata agataaatgg gatgctttat agcgaaaatt cattaggaca aagtacacaa
     9841 ctactgatac aacaacaact gactggcttg caatcgatgc ttcgagcgag tcaattaggc
     9901 atttcaccag aagaagtagc tagcttgagc caacaagcaa gtttgactcg acaaaaagtc
     9961 aattttgatt caaaaggaaa aatgacagtc ggagaagata actcgacagt ccaatacgtc
    10021 gtaagttatg tagcaacgat tgtcttgttc attattattt tgacgtatgc gcagatcatt
    10081 gcacaagaga tcgcttctga aaaaggcacg cggatcatgg aagtgatcct gtccagtacg
    10141 actgcacaaa agcattttta cggaaaatta acaggtgttt tacttgttgc attaacgcaa
    10201 atggcattat acggtattat ttttggtata ggatacagtc aattcaaaga tatggaaatg
    10261 gtcaaaaata tattagacgg tatctcattg gataacttga ttggaccatt cttgtggtat
    10321 tctcttctat tcatgatctt tggcattttg attttttctg ttttagcagc tttatgtggc
    10381 tctttggtca acaaagcaga agatacagca aaagcaattt tgccagtgac ttatctatct
    10441 ctaggcggat acatgcttgg attgatacta ggagcttcag atccaaacaa tatcgttatt
    10501 cggatcactt cgtatattcc attcttatct tcatatatta tgccgattcg cttagcaaat
    10561 gagacagtcg gtatgggtgg agcattgatt tccttgttgg tacttgtagc cgtaacattc
    10621 ttattgatga tcttgtctgc aaacatgtac aaatcgaatg tactcgttta tagcgaaggc
    10681 ggattatgga gttcattgaa acaatcgatt tcaattatga ggaatgaacg aaaaaaagca
    10741 taaaacaatc ctaaaataca gaaggaagct ggacatctat tgttcccagc ttctttttgt
    10801 atagacctat tttatttatt ttactatatt ggatatatat tcattgaaaa tagtgtataa
    10861 caactttata atattgagta taccaatttt tggaggcgtt ttaatggacc gttatttaga
    10921 tttactttca gaaaaattcc cctcaacgga ggatgtcatc acggaaatca tcaatctcga
    10981 agcaattttg cagcttccaa aaggaacaga gttatttttg agtgatatac atggcgaatt
    11041 tcctgctttt gatcatattc tgcgtatcgg ttcaggaaat cttaaagaaa agatcaaaga
    11101 ccttttttct gaacaaatga cggaaacgga tatcagccaa ttcactattt ttacggctta
    11161 tccagaatat gcattaacga ctgattggta caaagaacaa gataaatcgc agctgatcca
    11221 tcatttgatc gatcttttac ggtttacgac agtcaaatat acccggtcaa aagttcgaaa
    11281 aggattgccg aaagaatata gctatatcat tgaggaactc ttgtatatcg atgaccgtat
    11341 caatggaaaa aaagactatg taaaaaaaat cattgaacaa cttgttttga tgaaggaaga
    11401 agaaaaattt ttaacgaaac ttgcacagac gatccaaaaa tctgtcatcg atcatttgca
    11461 tatcgttgga gatatttttg accgaggaac tcaagcagcc aaagtgatgg atcgaatcat
    11521 ggatttttct tctgtagata tccaatgggg aaatcatgat attttgtgga ttggtgcgta
    11581 ttgcggctct gaggcatgtt tactgacact tttacggatt gcagcacgct ataattattt
    11641 atttgaatta gagaaagaat atgggttgaa tttacgtcca ttgttttctt ttgctcatca
    11701 aacttatcag aagaatccag tatttgcgcc taaaaacagt aaaaatctat ccgaaagaga
    11761 gcaagaagaa ttagaaaaaa tccatcaggc actttctatc cttcaattca aatcagaaca
    11821 tcagttattg gatcgtcgac ctgaatttaa aatggacgat cgtaaattgc tggaaaaaat
    11881 cgactatgaa gcatcaacga ttgatttaga aggtcagctc tatgagttga aaggaacttg
    11941 ttttcaaacg atccagccag atgatccaaa aaaattcttg gaagaggaac aaaaagtgat
    12001 tgatagtttg atgtattcat tccagcactc tttgagaatg caaaaacata tgacgttttt
    12061 gatcgaaaaa gggggaatgt atctcacgaa caatcagcat gtgctgtttc atggctgtat
    12121 acctgtagac gagaagggac agctcttagc atttgaatta gaccaatctt atcgcggaaa
    12181 agaactgctg ggcttttttg aaaaacagat aactgaaagc gcaaaagatc tgactgcaaa
    12241 agaagattta gcaaccgatt tgatttggta tgcgtggagt gggccatttt cgcctttatt
    12301 cggcaagagt aaaatggcga cttttgaacg ttattttctc acagagcctc atacgcacgt
    12361 cgagaaaagc aatccttatt atcacttgcg agatgaagag tggttcagtg aaaaaatttt
    12421 agcagaattc gacacaaaag aagaagggtc agcaatcatt aatgggcaca cgccagtaaa
    12481 agtaaaaaaa ggggagtcac cgatcaaagc aaatggaaaa gtatttgtca ttgacggagg
    12541 tttatccaaa gcttatcaaa aaacgacagg aattgccggt tattcactat tatgcaattc
    12601 ttatggtttc caaatcgtga cacattatcc gtttttgcct attgaacaac aatttgcaaa
    12661 aaaatccgac caaacaaatg tgaaaaaagt aatcgaacag caactaccac gcaaactcaa
    12721 tcgtgataca acaaaaggta tggaactgca gcagcagatt acccagctca aaaggctgtt
    12781 ggactatcga aaggacgatt gagaagaatt ccagtgaaca ttgttccata gactggagac
    12841 tgagacaaaa gtcgtgtcgc agtctctttt tccggataaa cggtgttctt ctgccaactc
    12901 ctcggtcaca agtcacacta ttcaacaaac atgaaaagct gttttttgaa attgctcctt
    12961 gtgactcaga gttgaacggc aggctcacga cctcttggta taaacggtgt tcaaaagcca
    13021 gtttctcggt attaagccaa atttatgcaa aaatgtgaaa agccattttc acaaaatttt
    13081 tcttaatact cgaaactaaa cagctttttc acaaccccct cagaaagtct attaaaattc
    13141 attcacaaag aaatcgaata agcgctgttc agatggatgt acacggtggg taagttccgg
    13201 atgccattga acaccgagga ttttatgcat atcatctttg ctttgaaccg cttcaacgag
    13261 tccatcgtta gaaaaagcga tggcttttaa taaaggtgaa agatctttga ctgcctgatg
    13321 atggtaagaa ttcacaaaat atggatcggg gagaaggttg cttaacaaac tgtctgaaac
    13381 aatcttgact tcgtgagtcg taaattgcgg ggcagtgggc tgttgttcat gttttactga
    13441 ccactttgga tataatgaaa gatcttgata aagtgtacct tccaaaacga cattcaatag
    13501 ttggagtccc cggcagactg caaaaattgg ttttctttgt ttcaatgctt ccaagatcag
    13561 tgcctgttca aattggtctc gttgaatatt tgtttccgtt agctttggat gaggatcttc
    13621 catgtaaagt tgtggactga catcttgacc acctgcaaga ataagtttgt cgatttgtcc
    13681 aatcagttga ggagctgaat ctggagacat gactggcaga ataagaggca atccttgagc
    13741 atctaaaact gcatccacga atccttgcgg tgtatagctc acttgatttc cttggaaagt
    13801 atctgttgct cgaatcagtt gatttccggc aatgccgatg attggtttca tttgtattcc
    13861 ccctaaagta gacttattgt aacaaggatt taaaaaaaat taaactatgt aagacttgcc
    13921 cctatactta tctgttcttg ctgtgttact atctgtaaag agaaatttcg tgtgaaattc
    13981 atgacgatag ttgaaaatat tgcttaattt gttataataa aaagtaagca gaaataaaaa
    14041 ggttgggcgt aagcttcaag gatctagttc ttcaaggggt gagaaggaca ttttattgaa
    14101 ggggcctgcc tatactaagt tatctttaga ggtacatact ttcaatgaaa aaagaaagca
    14161 tgtacctctt ttttaaatcg aaaggaagtg aagcaagtgt tagaagcttt ggaacaaatc
    14221 cgaaaagcag aagaagaaaa tgaaaagcgt gctcaaacac tgcggcagca gctgaagatc
    14281 tatgcaacag aaaaagagca ggaattggaa acgatccaaa caaactatcg acaacaacta
    14341 aaacagttgg tggaagaaaa agaacaattt gaaatacaag aattagaaaa agaaaaaatg
    14401 aaattgaaag cagaagcaga caaggtgcaa gcaactttga aagatcaata tcaaaagcat
    14461 cagagtcaag cgatcggtgc gatcattgaa agggtgaaag aaacttatgg ccgtcactaa
    14521 gatggaaaaa gtgaccctca tttcagataa aagaaatcag gagaggattt tacaagcagt
    14581 acaaggaatc caaaacgttg aaatccggga tctgtatcaa aatagaacaa acaaccagtg
    14641 ggtggagaag tattttccag ataccacgat tattgataaa gaagcaaaat taagcgcttt
    14701 aggtaatcgg ttgaaagaca tccaagaagc tatccaattc atacaacacc acggcgatag
    14761 ccgtcaaaag aaactccatt tgaaacgtca agaactttcc ttacaagatt tggaagccaa
    14821 ttattccgaa gaaaatttca taaataaatt gaaagaagtc cttgatttaa aagaaaagtg
    14881 ggaacaacta actgaagaac gtgatcgttt agaagaagag gaagaatggc ttcttcattg
    14941 gcaacagctg gatattgctc caagtatgta tgcaagtaag acgaccattt ttgagatggg
    15001 gactgtgagt gcagcaaatt ttgaaagttt caaggaagaa ctgagtaagc ttgatgacgt
    15061 ttatatggaa gaaatagatt atacgccaaa acaagtttat ctttcgtatg ttgctttaaa
    15121 aaaatcagtt gctgttatag aagaaattgc tgggaaatac gggttcaata aagaaacgta
    15181 tccttatgaa caaaatccta aagaaaaact gcaagagacc aagcagcaat tatccattgt
    15241 tcacgagcag caaaagaaga taacgacagc gttaggtcag tgcagtggct atatccaaga
    15301 atttgaatgg gtagaagaag tcacacttgc cgttgcagaa cgagaaaaaa taaaagaacg
    15361 gtttatccat gcgacttatc tgatcgtttt gcaaggttgg gtagatgcag aagaaaaaca
    15421 gaatttgctg catgtgttaa acgaaaccgt atcagaagaa gatatatacg tcagtttcga
    15481 aacaccaaac tcagaagaga ttcagcagga agtccctact aaattgaaaa accatccgct
    15541 tgtcgaacct tttgaattgc tgactgaaat gtacagtctg ccaaaatatg aagaagtaga
    15601 tccaacacct tggatgacac cattttatct tgtcttcttc gggatgatgg tcgcagatgt
    15661 tggttatggt ttgctgatgc tcattgggac aatactagct caaaaactac ttgttctacc
    15721 acgcgggatg aaacgtttcg taaaattctt tgagattctc tcgataccat cgatcatctg
    15781 gggattgatc tacagttcat ttttcggaca gtcattacca aaagaactgt ttggcatccg
    15841 cttgcctttc ccaatcctat ctacgacgga tgacgtcaat acgattttga ttttatccgt
    15901 gattttcggt ctgatacaga ttttggttgg attatttgtg gcagcaaagg aaaatatcag
    15961 gagaaaagat tacttgagtg ctgtcagtga tggatttgcc tggcaaggga tcctaattgg
    16021 gatcgttatt gcgttggttg gtgccatgtt gataaaaaat acagcatttg tttaccttgg
    16081 cgctgggcta tcgatcattg ctgccttatg tatcttgatc gttccgatta tccaaacgcc
    16141 gtctaaagtc aaaggtgctg ccaaaggtgc gtataatctt tacggactga ccggctatat
    16201 cggtgattta gtcagttata cacgtttgat ggcattgggt atttcaggcg gaagtattgg
    16261 agcggcattc aatatgctag ttgctttcat gccgccagct gctcgattca gtgtagggat
    16321 ccttttgatc atcgctttac acgcactgaa tatgtttttg acactgctaa gtgcctatgt
    16381 ccatggcgca cgattgcagt atgtcgaatt ttttgggaag ttttatacag gaggcggacg
    16441 tgcctttgat ccgctgaaaa cggcggaaaa atacgtcaac atcaatcaca agaaaaaaaa
    16501 caataaataa ttggaggatt tttatataat gatggattac ttaattagtc aaaatggtgg
    16561 aatggtattt gcggtattag cgatggcaac agcgacgatt ttttcaggga tcggttcagc
    16621 taaaggtgtt ggaatgacag gtgaggcagc agcagcattg acaacaagtc aacccgaaaa
    16681 attcgggcag gctctgattc tgcaattgct tccaggcacg caaggtttgt atggattcgt
    16741 tattgccttt ttgattttta tcaacttagg aaatgacatg tcagttgtac aaggactgaa
    16801 ctttctaggt gcttctctgc cgattgcttt tacaggcttg ttttcaggta ttgcccaagg
    16861 aaaagtcgca gcggcaggta ttcaaatttt agcgaaaaaa cctgaacatg ctacaaaagg
    16921 aatcattttt gctgcgatgg ttgaaacata cgcgatttta ggtttcgtta tttccttcct
    16981 attagtgttg aatgcctaag cataaaggag gaaaagcaga tgaacgccat tgaaaaaatc
    17041 atctctcaga tgaacgaagc agcagagcag gaaagagcgg cgttagaaca agaagagcga
    17101 atgaagatcg atcaaaactt tgaacaaaaa cgtacgcaag ttgaaacaga acatcaaaaa
    17161 caaaaagaaa aacaaatcga attactcgaa aaaaaatacc gtcagctaag gaatcgccag
    17221 caagtagaag tacgacaaga aaatttgaat gcgaagcagg aatttttaag acgattgttt
    17281 gcagatgcgg taactgaaat ggaaaattgg gatgaatctg aacaaatcca gtttatcaaa
    17341 aatgcattgt attcattacc tttgacagga aaagtagcat tcattgctgg agaaaaatca
    17401 gcagcctatc tttctcagac gttgttggat gaatggaaca atgaacttcc ctttatgatg
    17461 gttttaagtg atgaaactgt tgctgatcaa gcgggttttc tgataaacga tcaaggagtt
    17521 cagtataatt ttcttttcag cagccttgta caagatatcc aaggaacgat gagttttgag
    17581 attgccaatc aattattcga ataaggaggg atcagtgtat ggaataccac gaactaaatc
    17641 ccttgattcg cggaagagag cttgaacttt tgtccaatga gacatttgaa cggatgattc
    17701 aaacaaattc tgtcgaagca cttggtgaaa tattgaaatc cacgatttac agtccatata
    17761 tttatgacgg atatgaatat gacttcgaac agaatctttt aaaggagcag agccgtctat
    17821 ttcgctggtt aaaggaatca gcaccggagc ctgaaatcgt ttggatctat acgatgcgtt
    17881 atactttcca taatctgaag gttttgacaa aagctgagat gacaaatcaa aatctggacc
    17941 atttgtatat cgacgatgga ttttattcat tagaaacctt gaaggatgcc attcatacgc
    18001 aagtttcaaa tgaacttcct gaaagcatta tggacagtat acgagaagta cgtgattatt
    18061 ttgaagagtc taccattctt cagggaattg acgtgattta tgatcgacag tttttgacag
    18121 agcagcgacg gcttgccgat aagctaggat atcaagaatt gctgacagaa gtcattgctt
    18181 ttattgactt gaccaatatc acaacgatgg ctcgaggaat attgcagaaa agaacatatg
    18241 ggttcatgac gacggtactt tccagctcgg gaagtatcga aaaagaagta tttttgaact
    18301 ttgtagataa agatatggaa gattacacga attttctatt agagacagat tatgctgatt
    18361 ttctgcgccc tgtcatccgt gagggaaata ttgatctcgt aaagcttgaa cagttgaaag
    18421 atgattatct aagttcattg tatcaagaag cacaaaccca ggctttcgga ccactgccac
    18481 tactagcctt tttgaacgca aaagaggtag aaagcaaaaa ccttcgttta ctggttatcg
    18541 gcaaaagaag ccatttcgaa tcagaaacaa tcagaaagcg ggtgagacaa gtctatgacg
    18601 cataagatcg gtgtagttgg cgacaaagat tctgttttgc cattcaaatt gtttggcttt
    18661 gatgttcgtt atggtacaac caagcaaacc gttcgtcagg caattgaaga aatggcaaag
    18721 aatgagtatg gcgtgattta tgtcacagag caatgtgcag cattgagccc agaaacgatc
    18781 gaacgttaca aggaacaaat gatacctgct atcgtattga ttcccaatca tcaaggatca
    18841 ttagggatag ggctggaaga aattcaaaaa aacgtagaaa aagcagtagg tcaaaatatc
    18901 ttatagtatg aaaaggagat agaaattttg caaattggaa aaatcataaa agtctccgga
    18961 cctcttgtaa tggctgagaa catgtctgat gccagtatcc aagacatgtg tctagtggga
    19021 gatctaggag ttatcggaga aattatcgag atgcgtggcg atgtggcgtc tattcaagta
    19081 tatgaagaga cttctggaat tggtccaggc gaaccggttc gctctactgg tgaagcttta
    19141 tctgttgaat taggaccagg gatcatttca caaatgtttg acggaatcca acggccatta
    19201 gataccttta tggaaatcac acaaagtaat ttcttagggc gcggagttca attgcccgca
    19261 ttggaccatg agaaaaaatg gtggttcgaa ccaacagtag aagccggaga aacggtgagt
    19321 gctggagatg ttatcgggat cgtagaagaa acaaaagtta taaaacataa aatcatggtg
    19381 ccaaatggga taaaaggaac gatcaaaaca atcgaaaaag gttcattcac tattgatgaa
    19441 acagtatgtg tcattgaaac cgaaaatggt gacaaaaatt tgacgatgct tcaaaaatgg
    19501 ccagtaagac gtagtcggcc aatcaaagaa aaattaaacc cggatgcacc catgatcact
    19561 ggacagcgag tgattgatac attcttccca gtgacaaaag gaggagcagc agctgtacct
    19621 ggtccttttg gtgcagggaa aacagtcgtt cagcatcaaa ttgcaaaatg ggccgatgtg
    19681 gacatggttg tctatgtggg atgtggcgag cgtggaaatg agatgacgga tgttttaaat
    19741 gaattccctg aattgattga ccctaataca ggagaatcgt tgatggaacg tacggtattg
    19801 attgctaata catcaaacat gccagtagca gctcgagaag cttctatcta tacgggaatc
    19861 acaattgcgg aatatttccg tgacatgggt tatgacgttg ctatcatggc agactccact
    19921 tcacgatggg cagaagcact tcgtgagatg agcggacgtt tggaagaaat gcctggtgat
    19981 gaaggttatc cagcttatct tggttcacgg cttgcggaat actatgaacg atctggaaga
    20041 gtcattgcat taggatcaga aggtcgcgaa ggaagtataa ccgcaatcag tgctgtatcc
    20101 ccgtctggag gggatatttc ggaaccagtc acacaaaata cattgcgggt cgtaaaagta
    20161 ttctggggtc ttgattccag tttagcccaa aaaagacatt ttccttcgat cgattggata
    20221 caaagctatt ctctttattc aacagaagta gggaagtata tggatcatat ccttcaagct
    20281 gactgggcat ccatggtcac cgagggcatg cgtattttgc aggaagaaca gcagctgaat
    20341 gaaatcgtgc gtttggtagg gattgattcc ctttcagata atgaccgtct gacattagaa
    20401 gtggcaaaat ccattcgtga agattatttg cagcaaaatg cttttgatga tgtcgataca
    20461 ttcacttcaa gagagaaaca attcaaaatg ttgaagatga tcttgacctt tggtgaagaa
    20521 ggacgccgtg cgttgtcgtt aggtgcttat ttaagtgaga tcatggacgg taccgtatcg
    20581 attcgtgaaa ggatcggacg aagcaaatat atcccggaaa cagaattaga aaaactagat
    20641 caaattattg gtgaaatcaa gcaaacgatg caaacgatcg tttcagaagg aggcatgagc
    20701 aatgattaaa gaatatcgta cgatcggtga agtcgtcggt ccattgatgg cggttgaaaa
    20761 agttgctggt gtcaaatatg aagaattaat cgaagtccgt atgcaaaatg gcgagattcg
    20821 tcgtggacaa gtattggaag tccaagaaga taaagcaatg gtccaaatat tcgaaggaac
    20881 aagtggtatc aatttacgtg actcttctgt acgattttta ggccatcctt tagaactagg
    20941 tgtatcggaa gacatgatcg gtcgagtatt cgatggtttg ggacgaccga aagataacgg
    21001 accagagatc ctgcctgaaa agcatttgga tattaacgga gaagtaatca atccagtagc
    21061 tcgggattat ccagatgagt ttatccaaac aggtatctct gcaatcgatc atttgaatac
    21121 attagttcgt gggcaaaaat taccagtatt ttctggctct ggtcttcctc ataaagaatt
    21181 agctgctcag atcgcgcgac aagcaacggt actcgattca tcagatgatt tcgccgtagt
    21241 ctttgctgct attggaatca cttttgaaga agctgaattt ttcatggaag attttcgtca
    21301 aacgggcgca atcgaccgtt ctgtcatgtt tatgaattta gcaaacgacc ctgcaatcga
    21361 acgtatcgct actcctcgta tggctttgac tgctgcagaa tatcttgctt atgaaaaggg
    21421 aatgcatgtt ttggttatca tgacagacat gaccaactat gcagaggcat tgcgagagat
    21481 ttctgcagca cggcgagaag ttccaggaag aagaggatat cctgggtatc tttacacgaa
    21541 cttagctact ttattcgaac gagcaggaag aatccgcgga ctaaaaggat cggttactca
    21601 aattccgatt ctaacaatgc cagaagacga taaaactcat ccaatccctg acttgactgg
    21661 ttatattaca gaaggacaga tcatcttgac tagagagctt tacaagagtg ggattcagcc
    21721 accaattgat gtactcccgt ctctttcgcg gttaaaagat aagggaactg gacccggaaa
    21781 gacaagagaa gaccatgcag ctacgatgaa tcagcttttt gcagcctatg cacaaggaaa
    21841 acaggcaaaa gaattagccg tagttttagg tgaatctgct ttatctgatg tagacaagat
    21901 ctatgcgaag tttgctgagc ggtttgaaaa agaatatgtc aatcaaggat tctacacgaa
    21961 ccgttcgatt actgaaacac ttgatctagg gtgggaactg ctatcgatgt tgccaagaac
    22021 cgaattaaaa cgaatcaaag acgacatgct ggacaaatat ctgccagaag ggaagtgagt
    22081 tctgatggca cgattgaatg tgaatccgac ccgcatggaa ttgactcgat tgaaaaaaca
    22141 gttgacaact gccacaagag ggcataaatt attaaaagat aagcaagatg aactgatgcg
    22201 tcaattcatc ctgttgattc gaaagaacaa ccagctgcgt caagaaatgg aagagtctat
    22261 gacagaagca atgtctgatt ttgtattggc aaatgcttcg atcacggaac ctttcatcga
    22321 agaattattt gttttgccgg cggaaaacgt tgatttgtct atcgtagaca aaaatatcat
    22381 gagtgtaaaa gtacctgtga tgaattttga atatgatgaa cagctgacag acacaccttt
    22441 ggaatacggg tatttgaatt ccagtgccga actggatcgg tcaatcgatc ggtttactac
    22501 cctgcttcca aaacttttgg aactgacaga aatcgaaaaa acttgtcaac tgatggcgag
    22561 tgaaatcgaa aagacacgtc ggcgagtcaa tgcattagaa tatatgacga tccctcaatt
    22621 agaagaaacg atctattata ttcgtatgaa gctggaagaa aacgagcgcg cagaagtaac
    22681 aaggttgatc aaagtaaaaa atatgggtac cactgactaa gtggtatctt tttttaaaag
    22741 aggaaaacga tgattaaaca aagcaaacaa ataaagaaac gtttgccggc tgttcagctt
    22801 ctcgcattag gattttttct cctgatttta gttggcggcg gcctacttac cctaccattt
    22861 tttagtaata gtgggcatgg gacaaatttt atcgatgcgc ttttcactgc aacttctgct
    22921 gtctgtgtaa cgggattgac aacattgaat acagcagagc attggaacag tgctggtcaa
    22981 tttttgatca tgcttttgat tgagatcggc ggtcttggtt ttatgatggt tccgattcta
    23041 tgctttgcat tgataaagaa gaaagtcagt ctcagcacac gtattatatt aaaagaagcg
    23101 ctgaatctag aagaagtatc aggtgtgatg aagctcatgc tttacatcct aaaattggcc
    23161 tttgtgatcc aattgattgg tacggttgct ttatcttttg tatttgtgcc tgaatttggc
    23221 tggttgaaag gtatctggta cagtttgttc catgcaatct ctagtttttg taatgcaggg
    23281 tttgatttgt taggagacag tttagccaat caccagcaaa atgtttattt gatcatggtg
    23341 gtatctgctt tgatcatttc tggtgggtta ggcttcatcg tatggcgtga tttactggaa
    23401 tatcatcaga aaaagaagat gacgattcat tcaaaaatcg cattaaccgt tacaggtacc
    23461 cttttattag gcggattcgt tgtgttcttt ttcacagagc gcaatggggc aacgctagtg
    23521 gaagggaata tgtttcaacg attggcaaac acctttttta tgagtgtgac acctagaacg
    23581 gctggatatt attcagttga ttacctccat atgagccatg caggtctgat tttaacgatg
    23641 tttttgatgt acatcggcgg aacatctggg tcgacggccg gcggattgaa aacaactacg
    23701 ttaggtgtgt tgctgatcca gatgcgttcg atgttgaaag gacgtacgag agcggaagca
    23761 tttggccgta ccatccgaca aggcgcagtg ttgcgggcac ttactttatt ttttgttacg
    23821 ttgacgttgt gtgtagtcgc catcatgatt ttatcggtga ctgaaacgat acctaaaact
    23881 tctggcatcg aatatattgc ttttgaggtc ttctctgcgt ttggtacagt aggtcttacg
    23941 atgggattga cgccagactt gactgcaata gggaaattga tcattatagc gcttatgtac
    24001 attggacgag ttggtgtatt gactgtggtc ttctctctga tgacaaaagc acaaagaaca
    24061 caatcaaaat acaaatatcc agaagaaagt gtcatgattg gataatttca tcttctgaca
    24121 gaaacctagt gatttagtaa aataagaaaa taaaataatt tttcttgctt cttggatact
    24181 ttaagtgcaa aatgttcgtt tattttttga ggcactaccc gttgttgaac ctcgggtagt
    24241 gcctcttttt tattgaaagg aatcaataag atttgattcc tttatttatc aataggttat
    24301 aattcatatt tttttatttt ttcttttacg atacttagtc cagaagctaa aatttcaagg
    24361 ttattagagg atagggaaat tcgaacgaat gcatctggtt gcgcacgtgt agtaaagcgt
    24421 ctggaatgat aaagtcggat accgttagca agaaagtcat tctcaattat cttagggtca
    24481 atctcaggat caactggaag aattcgatag taagggacac gattcgttgg tttttctact
    24541 ttgaaaagag aatcaaaaag ctgattggct ctttttgtaa gacttaattt agtttctaag
    24601 atcctatttg cttcatttga atggaggatt tgagtgatga cttccacatc taatccagaa
    24661 gttttcacat ttatattaaa aattgcttgt tctatcttgc tttggacatg tactggaact
    24721 acaagataag caactcgcaa tccagaacaa ataaattttg tcattccagc taagtagatt
    24781 gtttggttag gtaataaatt ttggaaagga ggaatgatcg tatgttgata gtaagttgtt
    24841 aaaaatgaat ggatatcatc ttcaataacc catagacctt ctttttcaat cacttcggct
    24901 aattgctctc gtcttttgtg agagatttga aaccctatag gattattaca agaaggcata
    24961 agaaagacac catgaatctt cttttttcta cattcctgaa tcaatatatc aggtcgcatt
    25021 ccttgctcgt caaaatcaat tggaacgatc tctaaatgat ataactgagc caattcaatg
    25081 aaattagcgt atgtatatcg gtctgttgct attctatcac ctggagagaa aagtgcagaa
    25141 agtgtgatag ccaatccatt ttgaacaccg gaaacaatag atagagattc taaggacgga
    25201 atgactcctt gagtttttaa ccaatttgct gcagtagtca attgaaaatt tgtgcctttg
    25261 ggatcttggt aattaagcaa accaacaaga tttgggttat tactagttga ttgaataaac
    25321 gggacaacga atttattaca ttcttcaaaa gaactaacca gaccaaaatc aatgatatgt
    25381 tcggttactt cattggtaga gatggtgatg gactcaaaga cattgggaga aataaatgtg
    25441 ccacttccaa tattcgtata cagcaaacct ttctcaattc catatttata agccttgcca
    25501 acggtagtaa aatttaaatc taaataatca gctagctctc tttgggaggg taactgagtg
    25561 tttttctgta atttattctt taaaatatct tgttctaatt gttgaatcaa cgatcgataa
    25621 taaggacgct tcaatttttg tttgtctggt cgccaagtta acgggtaatt gtcataagaa
    25681 ttaataggca ttttctccac tccttaaaat tgtataccat acaattttac cattgattgt
    25741 attgctttta gaagttaatc ttttgtgagg agggaattgt ctatgaaaat tattaatttt
    25801 gaggaagtag tagcttcttt tagttttgaa gaagcgattc aagtaatgaa aaaatgtttt
    25861 tttgattatg aaagtggtaa aatcagccaa aatcctcgat tggtggaaat tttacctgac
    25921 ggaaaaggca aaaatatgtt tgcgatgatg cctgcttatt tgggggagaa cagatatttt
    25981 ggcacaaaag tcattaccgc ttttccggat aatcatcagt atcatttacc ttctcattta
    26041 ggagagattt tcttgtttga tgctcaaaac ggactaccca aagcattgat aaacgcaaat
    26101 gcagtcacct ggattcgaac agctgctgta tctgcgctgg caacagatta cttagcaaat
    26161 ccgcatgcag aaacacttgc tttaattgga gctggtcaac aagcattgtc gcatttgact
    26221 gcgatttcta ccattcgtcc catcactaca gtgtttgttt acgatatttt agaagaaaga
    26281 cgaaatactt ttatcgctaa agctaaaaaa cagtatccta aaataacgtt tataaatgca
    26341 atatcagtag aagatgcagt acgtccagcg gaaatcattt gtacgttgac ctctagtaaa
    26401 gaggcatttc ttcaaaaaga gtgggtaagg gaagacgcac atatcaatgc tgtgggcaca
    26461 tttactcctg atacaagaga gataagtaca tcgttgatgg aaacttctca aatctatgta
    26521 gatgattact cagctgcctt aacagaaagc ggggacctgc ttatagccat ggcagaagga
    26581 gatttagagg aatcttgtat tgttggtaca ttaggcgaat tagtttctga gaaaaaaaca
    26641 ttttataaag gagaatcgag gccgacgatt ttcgacgcag ttggtctagc tattgaagat
    26701 ctttgttgtg cagaatatat ttataacaaa ttagagaaag gggctttaaa atgaacttag
    26761 attattacgt agttgatgca tttgcagatg aggtgttcaa aggaaatcca gcagcggttt
    26821 atgtgctaga agaatggctg ccagaaggta cgatgcaaaa aattgccatt gaaaacaatt
    26881 tatctgaaac agcatttaca gtgaagaaaa accaagaatt tgagcttaga tggtttacgc
    26941 cagatcgaga aatcgatctt tgtggtcacg cgacattagc aacagcattt gttttattta
    27001 actattacaa aatacctgat gaaacgatta aattttctac tcaaagcggt aatctatttg
    27061 tgacaagaat taaagattat tattacatgg attttcctag tattatgccg aaaaaagtgc
    27121 cgatattgtc tgaatatgaa gaagcgattg gggctaagat taaagaagcc tacttagcac
    27181 gtgatttatt tttcgtatta gaagatgaaa aaacggtggc taaattgcag ccagatttta
    27241 cagctatcaa aaattttgaa ttaggaatag gagtcatcgt gacagcagaa agtgtgcaaa
    27301 aagactttgt ttctagaacc ttctttccaa aattaacaat caatgaagat ccagtatgtg
    27361 gatcagcaca ttctaatctt attccttatt gggcagaaag aatgaataaa acaaaattgt
    27421 cagcttatca aatgtcatca aggggcgggg atctagaatg tgaattaaaa gaagatcgtg
    27481 tgataatagg aggaaaagca gttttgtttt ctaaaggtac ctcgtttatt tgatttcttt
    27541 caaatgaatt aaaaataaat tggtggagag tggcaacctt atagctacaa tggttgttaa
    27601 aaaaagacgc aaactttttc aatttaatga aaagtttgcg tctcatttta attattggtg
    27661 tttgtttttt attcaatcaa gagtaaaatg ccccatacta actatcctcg gatgacttcg
    27721 attttataac catcagggtc aatgacaaag tagtaagaag gcgcagtgcc aggtaatcct
    27781 tttaagtcag taatctcaag accagcagct ttatgttgct cgtgtaattt ttctagatca
    27841 tcagctgcta tagcgatatg accatagccg tctcctagat catacgcacc atgatcatag
    27901 ttgtaagtca actctaattc atagtcatcc ccaggaagtg tcaaataaac aagtgtaaat
    27961 ttgttttctg gaaaatcacg gcggcggctt tcttcaaaac caaatgcctt ttgataaaat
    28021 tcgattgatg cttcaagatc tttcactcga acacatgtgt gagccatttt catagattca
    28081 tctctccttt tccttttact caatcataac aaaaagaagt aaggaaatag ttgatttgtg
    28141 cttgttcatg agaaatttct catgtaaaat ggatagaaaa taaaactgtt aaggtggaaa
    28201 ctattcatgg ataaaccaat ttttggtgaa aaaaagcaat ttgctgatta tcaaacccgt
    28261 tatgcagcat atattgtcat agaaagagat cagcagattg cagtaatcga ggctccaaat
    28321 ggtgcttttt ttcttcctgg cggggaaatt gaaggagaag agacgaaaga agaagcgatt
    28381 gtccgtgagc tgatagaaga aatgggaatt gtggcagaaa tttcttctta tctgggagaa
    28441 gctgatgagt atttttactc gaattatcgt gcaacttatt attataatcc aggatatttc
    28501 tttgttgctg attcttggaa gcgtatagga gaaccaactg aaaagaccaa taaaatttgg
    28561 tgggtgagcc ctcaagaagc gcttgtgaaa ttgaaacgtg gtagccaccg atgggcagtc
    28621 gagagatgga tcgaaaaaaa taaaaactga aagaaagcga gtagatcagc tttctctcag
    28681 tttttttatt tattacagta catatttttt gattgcttca gctacaccgt gctcgtcgtt
    28741 tgatgaagtg atgacatttg ctgcttcttt gacaagagaa acggcatttc ccattgctac
    28801 tcctagacca gcatattcaa tcatggataa gtcgttctcg ttatctccga tagccatgat
    28861 ttcatcttga tcaataccaa ggtgctgtgc gagattcgca actgccgcac ctttacttgc
    28921 ttctttattt agtacttcga agtaataggg cgcacttttg accgttgtgt atttatcacg
    28981 gaaagtttgc ggtaaacgcg caattgctgc atctaaaatt tctggttcgt cgatcatcat
    29041 cattttgacg atgttcatgt ctggtgtcat ttcttcaggt gttcgatatt ttagcggcat
    29101 cttgactaaa cttgcttcgt ggatcgtata agcgctgata tctcgattag gcgtgtagat
    29161 cgctttgtca tcgatcgaat ggagatgact gccgacacgg cgtgccatta cttcgatctc
    29221 taggaaatct tcatgtgtca gtccgtgacg ggcgatgatt ttacctgatt tcgtttgttg
    29281 tacgagtgcc ccgttataag taataacgta atcattatct tgaaaaaggt tcaactcgtt
    29341 taactgttcc tggacacctg gtaagggacg gccagtgcat aaaacgatcg aaacaccttt
    29401 tgcaacagca gcggcaatcg cttcttttac ttcttttgtt acttctcttt tctcatttaa
    29461 taaagtacca tcgatgtcga tagcgactag tttgatagac ataatttcct ccaatatatt
    29521 ttaatttatt tcaatcaatg cgccattacg gatatggctg gcaaattctt gataagtttc
    29581 gtcaaataaa tcataatgat cacgcaagga cggatcaacc atttcttttg gaaagaaaaa
    29641 tcgctcatct ccttgttctt gtcccgctaa tgcagcaaca agctgactaa cctgtgataa
    29701 ttctatcaaa gttccgtcat ttcgcaaaat ctcgatttgt gtgcgatgtc tgccttgttc
    29761 tgggcgataa aaatcataag gtagatcgta gctagaattc acagcagtgt aataaacagg
    29821 attatatccg accttttcta ctaacaaagt gagttcttgg actaattttg aatcccggtt
    29881 gccagaaaaa gtagcagatt tcagtggttt acgattcaaa aaacgtttag ctaaatcatt
    29941 taaaatagga tcagcagatt cagaccattg agtaaagtaa gtgcccaaga ccccatcatc
    30001 aagtttgagg tattcttcta aagtaaagct tccgtcaaga aatggcagaa gtaattgaga
    30061 atggagttca aaactgctgg catcttcttg atacaactcg tgtgcgcgat gaagcagatg
    30121 ctccaaaatt acttccatac cacgggaaac aggatggaag tacacttgca catacatttg
    30181 atatcggcta acgatataat cttccactgc atgcatgcca ctcatggaaa aagcgatccc
    30241 gccttcataa ggacgaatca cacgtaatat tcgggtaaga tcaaatgttc catattctgt
    30301 tccagtgaaa taagcatccc gaagcaaata gtccattcga tctgcatcga tctgactaga
    30361 aatcatttgg acaacttgtg ggttggggta agttttttgg atcacactag ctaccttttc
    30421 aggaaaacct tcttctactc ggttaagaat ctgatacact tctgtttctg gagaagtgat
    30481 gatctctaca gtgatggctt catgattcgt atggaagata tgttcaaatg tatgagagta
    30541 aggaccatga ccgacatcat gcaacagtgc cgcacataaa gtcaccagcc gttcctgatc
    30601 atcccagcct ccgttgccta ttttttcttt tgaaaaattt cgagaaaaga tatcgcagat
    30661 tcgtcttgaa atctcataaa cgcctaatga atgagtaaaa cggctatgtt ctgctccgtg
    30721 aaaagtgaaa gaagaagtgc ctaattgttt aatgcgtcgt agacgttgga cttctttcga
    30781 attgatcaaa tctaagatta cttgatgctg gacatggata tagttatgga ctggatcacg
    30841 aaaaactttt tccataggca gcatttgata tttataaggg gttgtcataa tgtagctcct
    30901 ttatgttatt acgttgttcc attctctgga tttggttgtt ccattgctcc agctggattt
    30961 ctttttttag ccagtcggta gaatcctgta aatcacttgc agaatacagc ttttcaaaag
    31021 cttttgcaaa tgcttctttg acttctttta caaaaagggg ttggtcaagc agttcttcaa
    31081 gtgttgccat tgtagcagga tccactgcag gatagccatt tgtaccaaag tcttctttta
    31141 aagcagattg ataaaatcgc ttcatgactt gcccacgttt ttcctgatta ccgttgacac
    31201 ttaagtacat cataacagaa atcccatttt tgatccgacg ctgagcgata ccggcaaatt
    31261 ttttgccatg gatgcttaaa tcataagttc caggacaata agaatgggtg atttcgaatg
    31321 cttgtatcgg tgcctttgga aaagcgtatt tagtcaatag gagcatttgc tcgtaagcgt
    31381 catctatggt tatttttttt gtgtcacttt gggggaaaat caaagaaata ttcaaaattc
    31441 cttcatctga tacgatccct aatccacctg aatttctgat cactggagcg taacctgcat
    31501 cttccagtgt ttggatcccg tcacttagaa aaggagttcg aacatccttc atacctaaga
    31561 tgactgtgca gtcatattgc cagaaatgta aaaagatttg ctggtgttct ttagaatatt
    31621 ccgttaatat atctgttaat gcaaaagggg aaaagtcgtt tttttgataa atgtgctgat
    31681 ctagtcgaat cgcagggttc attcaaccgg ctcctttgta taaattcata ttttctacaa
    31741 tattatactc taaaatcaaa gagttgtttt ggaaaaaagg ttttctgtaa aaatggacaa
    31801 gttggttgac aaaacaaaag aaactaggaa caatgtagct atgagaagta aaggagaatg
    31861 aataatggaa ttatcacaag gaacaccgat ttcaatcaaa ttgcgtacaa aagtaaaaca
    31921 gcaaggggaa gtgcaagatt tttattttga tttgaaaggg caaatggtga caattggcga
    31981 tacgctgtac atacgttata aagagattca ggaggagacg ggcgatgaga ttcctgtaac
    32041 gatcaaatta gttccagatg gtcatgtaca gctgatccgt gcaggagaaa tgcggatgcg
    32101 tttgaaattt ggctataaag aacggttaga aacatcctat cgaacaccat acggtatgat
    32161 ccagattgct acttttacga aagaactgca tgttagtttg aaagaccgtc ccacagcagg
    32221 gaaggttcga atcgattatg atctgttcat gggaccggaa aaaatcggcg aatattattt
    32281 gaccttggat tttacagcgt aaatataaaa gaaaagtttg attttttgtg tctgattttg
    32341 tatgataagg agtagactgt gaaaggacgt gtcctgattg gaaattagtg tatttgaagg
    32401 tgcaaacaaa agcgaattat caatgatcga agtagcacac gcgatcttag aacaacgtgg
    32461 cgatgtcatg gatttttctg atttagctaa ccagatccaa aactatcttg ggaaatcaga
    32521 cagtgaaatt cgtgattcac tcgcacaatt ttatactgac ttgaatattg acggcagctt
    32581 tatctcatta ggggataatc gttggggctt gcgttcatgg tatgctatcg attcgatcga
    32641 tgaagaagta aaccatggaa tggacgaaga ggatgaagat actccacgtc gccgtaaacg
    32701 caagaaagtc aacgccttta tcaatgatga tgaagacgcg atcgattaca atgacgatga
    32761 cccagaagat acagatctga cagaagaaga tgacgatgat ctgtttgatg acgatgatga
    32821 tgaagatgaa gaaatcgctg cttataattc tgacttgcaa gaaatcggag cagatgaccc
    32881 gacagatgat gaagaagact tgccaggaaa tatcgaagaa gatttaagta tcatagacga
    32941 agacgatgat gatgaagatt atgaagagga agaattttct gactctgaaa aagaataaac
    33001 attattttct tacgagaggt tgtgacagag acgatcagcc tatcagaata aggaggtgtg
    33061 acacgatttt tgtcggctct tcgtcaaatt gtgttggcga agacaaattt gaataaaact
    33121 tagtccgata tgcggcgcgt tctgtgcgcc gcatattttt ttagttttca aagatcagac
    33181 cttgttgtaa atagatccgt gcccgaaagt tataaaagtt gcgataacca aacgcaattc
    33241 gtttaatcag cttgatcttg ttattcagcc cctcagtgac gccgtttgag tggggcacct
    33301 taaaggcatt tgaaataccc gattggtagc gatggaagat cgtgaatttc tttttaaacg
    33361 actgaggtaa cgtagatgaa attgttttaa gggtttcttg aaagccttcc gta
//
LOCUS       DAZAID010000020        33313 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000020 DAZAID010000000
VERSION     DAZAID010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.020"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..415
                     /locus_tag="ACG9BE_000916"
     CDS             59..415
                     /locus_tag="ACG9BE_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN3031688.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            405..671
                     /locus_tag="ACG9BE_000917"
     CDS             405..671
                     /locus_tag="ACG9BE_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3031689.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            830..1831
                     /locus_tag="ACG9BE_000918"
     CDS             830..1831
                     /locus_tag="ACG9BE_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFN3031690.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(2201..2815)
                     /locus_tag="ACG9BE_000919"
                     /pseudo
     CDS             complement(2201..2815)
                     /locus_tag="ACG9BE_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3055..3300
                     /locus_tag="ACG9BE_000920"
     CDS             3055..3300
                     /locus_tag="ACG9BE_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031691.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            3398..3619
                     /locus_tag="ACG9BE_000921"
     CDS             3398..3619
                     /locus_tag="ACG9BE_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031692.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            3941..4621
                     /locus_tag="ACG9BE_000922"
                     /pseudo
     CDS             3941..4621
                     /locus_tag="ACG9BE_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            4761..5300
                     /locus_tag="ACG9BE_000923"
     CDS             4761..5300
                     /locus_tag="ACG9BE_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031693.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            6320..7489
                     /locus_tag="ACG9BE_000924"
     CDS             6320..7489
                     /locus_tag="ACG9BE_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN3031694.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            7962..8516
                     /locus_tag="ACG9BE_000925"
     CDS             7962..8516
                     /locus_tag="ACG9BE_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3031695.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(8689..9126)
                     /locus_tag="ACG9BE_000926"
     CDS             complement(8689..9126)
                     /locus_tag="ACG9BE_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3031696.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     IKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            9174..10121
                     /locus_tag="ACG9BE_000927"
                     /pseudo
     CDS             9174..10121
                     /locus_tag="ACG9BE_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            10392..10827
                     /locus_tag="ACG9BE_000928"
                     /pseudo
     CDS             10392..10827
                     /locus_tag="ACG9BE_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            10843..11676
                     /locus_tag="ACG9BE_000929"
     CDS             10843..11676
                     /locus_tag="ACG9BE_000929"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3031697.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            11921..12946
                     /locus_tag="ACG9BE_000930"
     CDS             11921..12946
                     /locus_tag="ACG9BE_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3031698.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            13084..13209
                     /locus_tag="ACG9BE_000931"
     CDS             13084..13209
                     /locus_tag="ACG9BE_000931"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031699.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            13234..14349
                     /locus_tag="ACG9BE_000932"
                     /pseudo
     CDS             13234..14349
                     /locus_tag="ACG9BE_000932"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            14432..15328
                     /locus_tag="ACG9BE_000933"
                     /pseudo
     CDS             14432..15328
                     /locus_tag="ACG9BE_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            15344..16168
                     /locus_tag="ACG9BE_000934"
     CDS             15344..16168
                     /locus_tag="ACG9BE_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3031700.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            16200..17510
                     /locus_tag="ACG9BE_000935"
     CDS             16200..17510
                     /locus_tag="ACG9BE_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFN3031701.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            17658..18917
                     /locus_tag="ACG9BE_000936"
     CDS             17658..18917
                     /locus_tag="ACG9BE_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN3031702.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            18927..19799
                     /locus_tag="ACG9BE_000937"
     CDS             18927..19799
                     /locus_tag="ACG9BE_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3031703.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            19811..20641
                     /locus_tag="ACG9BE_000938"
     CDS             19811..20641
                     /locus_tag="ACG9BE_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3031704.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            20681..22864
                     /locus_tag="ACG9BE_000939"
     CDS             20681..22864
                     /locus_tag="ACG9BE_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN3031705.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            22925..24145
                     /locus_tag="ACG9BE_000940"
     CDS             22925..24145
                     /locus_tag="ACG9BE_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFN3031706.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            24142..25602
                     /gene="gtfA"
                     /locus_tag="ACG9BE_000941"
     CDS             24142..25602
                     /gene="gtfA"
                     /locus_tag="ACG9BE_000941"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFN3031707.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            25679..26785
                     /locus_tag="ACG9BE_000942"
                     /pseudo
     CDS             25679..26785
                     /locus_tag="ACG9BE_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            27107..27316
                     /locus_tag="ACG9BE_000943"
     CDS             27107..27316
                     /locus_tag="ACG9BE_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN3031708.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            27309..27458
                     /locus_tag="ACG9BE_000944"
     CDS             27309..27458
                     /locus_tag="ACG9BE_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFN3031709.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            27612..28277
                     /locus_tag="ACG9BE_000945"
     CDS             27612..28277
                     /locus_tag="ACG9BE_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3031710.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(28561..29646)
                     /gene="xerS"
                     /locus_tag="ACG9BE_000946"
     CDS             complement(28561..29646)
                     /gene="xerS"
                     /locus_tag="ACG9BE_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFN3031711.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            29980..30213
                     /locus_tag="ACG9BE_000947"
     CDS             29980..30213
                     /locus_tag="ACG9BE_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFN3031712.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            30210..30617
                     /locus_tag="ACG9BE_000948"
     CDS             30210..30617
                     /locus_tag="ACG9BE_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFN3031713.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(31754..32272)
                     /locus_tag="ACG9BE_000949"
     CDS             complement(31754..32272)
                     /locus_tag="ACG9BE_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFN3031714.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            32639..32887
                     /locus_tag="ACG9BE_000950"
     CDS             32639..32887
                     /locus_tag="ACG9BE_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031715.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT        11554 a         4967 c         6110 g        10682 t
ORIGIN      
        1 aattcgaatt tttatgttat atactataat ataaaatcaa tatatgatat aattgggtat
       61 ggaaaaaata tcttttgaaa catttaaaag acctaatggt catgatgaat ttttagaatg
      121 gttggaaaca ttaccaaaaa aagatagtgc gaagctttta cgtacaatag aagaaacaga
      181 aaagaatggg atgctagtag ctcaacggtt gaaatgggtg aaaaaattag acactaattt
      241 gtacgaatta agaagtaagg taggatcaaa catccaaaga gctatctatt tccatgtggt
      301 caatggaagg tatgtcatta cccatggatt tactaaaaaa acggagaaaa caccaataag
      361 tgaacttaaa catgcaaaag aacttagaga ggagtggttc acaaatgatg attaaaaaat
      421 tgattgaaga aaagcaaaaa gacaaagatt ttgcagaagc atatgaccgt gaaggaagaa
      481 aactcgaaac tgcggttgct ctttatcatg cacgggagga agcaggccta actcaagcgg
      541 agcttgcaca aaaagcacat acaactcaat ctactattgc tcgaattgag agaggggata
      601 acgtatcttt cgataaactt gatgatatcg ctagggcatt aggcaaaaag ctggtagtag
      661 aatttgctta aaagcgagtt gaaaatatac ctgtaatagg ctcctaacta atcaattagt
      721 ttagtgggcc tttttgtact aaatagataa aagttgtcct atagtgtaaa agtcaataaa
      781 ttttatacaa aaattacttt aaagagacat aaagagaaag gtacaataaa tgaaaaccat
      841 aaatctaata aaagtattta tagcatcacc aggtgatata gcagcaaaga ggaatgaagt
      901 agaagaaacc atatatagat ggaatataga aaatacagat aatacaaaag taattttgat
      961 gccaattcga tgggaaacga atgcgattcc tacttatata aataatagct ctgggcaaag
     1021 tgttattaat aaacaaattg tagaaaatag tgatatatta ttagcgattt ttggccataa
     1081 gttgggttct ccagttgata attatagatc gggaactata gcagaaattg attactttta
     1141 taaaaatagc aaaggcgagg taggggtttt tttcttagat gaaccagttc cacaagtttt
     1201 ggctgaagag tatatagaag ttaagaagta cagggactct ttagaagata agggttttta
     1261 tggaacatat agcaaagaca atatcagaag gtttctcaca aaaaaagtga attatttaat
     1321 tgagaaagca caaaataatt tagaatcaaa acgctttgat ttagattcta tttcttacaa
     1381 gacaccttca gtaattaaca aaaaagttac cggaactgat aaagattttg atgacaactt
     1441 agatattttt gatccaatag aatttgacaa tgatgaaaga ttgctgctaa tatatgcgtt
     1501 tatggatgaa aattctatct tttcaactga taattcaact tatagaaaag tagttcaatg
     1561 ggaaagtgat aacaatttaa ggagttactt gagtgataga gtatttgatg tactagaaaa
     1621 attaagtaaa tcgggaattt taaaggaaga atacgattct tcttttgatg agtatccatc
     1681 acgctatatt ttgatttcag aggactataa gaaatttaaa aggtattttt taaaaaatga
     1741 aaaaaagata tctgttatta tggagaaata caaagctcca atacaggctg atccattttc
     1801 caatttggat gaatcagatt taccgtttta aattttaaat agataatttc attttttagt
     1861 agcagaggta taaacttttt taatgtttga attgaaaaga aatgctaata ggtcgacttt
     1921 atgtttctct tgtgcaatcg taaatgcggg ggaccataaa attgatctat tatcgagtgt
     1981 gtataaaata aaattcaaat cttattagtt tacataagat acattatact aagttgtcaa
     2041 tgttaaatgc ctttaaataa ggacgattta tgcatcaatt atatttatgt taactaaatt
     2101 ctttttaaca taaatgtaat caacttagct agatgaatct aaaatgattg tctgtattga
     2161 tgacaaagct aattaataat tacgtgattc aaaaaagaaa tcatacaatt agttggtcga
     2221 tttctttttt tattcggtaa attgtttgcc tagtcacatt gtagtctgtt gcaatctttg
     2281 agatagcaac cccattctca agatcctgaa cgatactcct atagacaagg cgacgttgag
     2341 gatctttggt gtctgcactg tataacttag gacgaccttt atagacgccg tttgctttag
     2401 cgattttaat tccttgtgcc tggcgtcgtt tcgattcagt acgttcttgt tcagcaatca
     2461 tggctgagat ttaaatgatt aagtctttga tgaatttatc taggagagga tttccaattg
     2521 cttcagccat gatcggtaaa ctggtgataa taagtcgcac attcttcttc tttagataat
     2581 tcactgaatc gataatctcg tcataattgc gtccaagtcg atcaatggct tctacaataa
     2641 aaatatcttg ctctctgaca aaatctaaag cttcctggaa aatcggccga tgactaatcg
     2701 tcgctccaga ttgtttttcg ataaaaattt tttcagcgcc aaactttttc atttcttcaa
     2761 tttgacgttg ttcattttga tcgatggatg aaactctaat ataagcaatt ttcaaattta
     2821 ttccctactt ttctataacg tttataatac accctaatca tacgttagaa ctctaaattt
     2881 ttcaatgtta tatttttgtc tatttagggt aaactctaaa tagacaaacg gtttttgaat
     2941 tctaaaagtt aaatagttta taatagaaga aaaagaatgg ggtgacgatt tcttagattg
     3001 aaaaggaaag ataaatatta tagattatta cttttgacag ctgaagcagg atggatggat
     3061 tttccagaag ttgtaaaaga agttcaagag ataggtgcaa ctatttctga tagccgctcc
     3121 tctcctatga actatcagga tgcccatgga attaaaataa ttgatcaaga gagaaatgtc
     3181 agaaaataca caacaataaa tgagtgtgta attaatgaaa atctttgtag ggcaccgata
     3241 acgaaacaca ttaagaatag aagcaaagca aaagacggaa gaacttttac taccttctaa
     3301 gtttttttca tatttgtttg ctatacttag atctaaggaa ttagactttc gctgcccatt
     3361 atttaagttt gaggtagcgt cttgcgaggg atcaataatg gaagaagaaa aaatatttga
     3421 aaaacgctgg gaattggctt cagttgaaca aagagcaagg taccataatc taatgtcctc
     3481 ttaccgtaat attgattgga cttacaaaga aaagaagtac ttgctttggt tatgtcagtt
     3541 agatgtaaat acctttgaaa catttgaagt gattctagat aagattaaaa acagcaatga
     3601 aaaaagagct gatctataat ggttcgctct ttttatttta ttaactcttt ggttactata
     3661 tgtaaaatac agcttcatat tacaataaat ttcaggatac aaatttattt aagcatatat
     3721 aaatataatt gaaataggac ataaatatat gagtatgaga aaggtgattc catgtttaat
     3781 aaatgggcaa accacccaat gaactcgaga ggtagagata atttgaagga gacggacaag
     3841 ttctgggaag aagtatatag taaaaacggt tctgttgcaa agttttccaa aaaatctatt
     3901 ttagtgtaaa attgagaaaa aagacagaga ggacagagta atgaatcatt ttaaaggcaa
     3961 acaattcaaa aaagacgtca ttattgtcgc tgttggttac tacctgcgtt acaatctaag
     4021 ctatcgtgaa gttcaggaat tgttatatga tcgtggaata aatgtttgtc atactacgat
     4081 ttatcgttgg gtataagagt acagcaaagt cctctattat ctttggaaga agaaaaatag
     4141 acaatccttc tattcatgga aaatggacga aacctatatc aaaattaagg gacgttggca
     4201 ttatctttat cgtgcaattg atgcggacgg cttaacctta gatatctggt tacgaaagaa
     4261 acgggatacg caagcagcct atgctttctt aaaacgactc cataaacagt ttggtgagcc
     4321 gaaagcaatt gtgaccgata aagcaccttc tcttggctcc gcctttagaa agttacagag
     4381 tgtgggttta tatactaaga cagagcaccg aactgtgaag tatcttaaca atttaataga
     4441 acaagaccat cgacctatta aacgacgaaa taaattttat caaagtctcc gtacagcctc
     4501 ttccacgatt aagggcatgg agaccattcg aggaatatat aaaaagaacc gaagaaatgg
     4561 aacgctcttc ggcttttcgg tgtctactga aatcaaggta ttaatgggaa taacagccta
     4621 agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca gaaccgagaa
     4681 ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt ttaaagtgga
     4741 aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt atttttactg
     4801 tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa ggtcgtttag
     4861 ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc ctatatgact
     4921 atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa gtaaaagttg
     4981 gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc tatacagtag
     5041 aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga acagttgtga
     5101 aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct attaaaggta
     5161 cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg attctatctt
     5221 tattaattag ttcaataact tttatattct tatttacacg ttatttaata agatgctgcg
     5281 gtgaaatgaa aatagattag atagatatta taaatatgtt gattctattt tcatagcaat
     5341 ggagaaaata cattagcaat atataaaaga aggtatcaag agtcacagtg aatttattcc
     5401 gaaaagtgaa aagagcaaat ataaaattta atgaaaatat agaactaaat aggaaaacgt
     5461 agacgtaatt ttctacgttt tccttttcat atatattaaa tattacggaa aattatataa
     5521 actaatttag tgttttgttt gtaattccta attttgctcc acttttagat aatttttagg
     5581 tctatttaac tcatcagaca ttctatgctc atttttctcc aaatttgttt caactctttc
     5641 gtacttaatt ttgcgtattt gaaaattctt cttttagagt acttttatta cgatactagt
     5701 atgatatata tctatagcaa acagttaaaa tcttcctaaa ataagcgaat agcattccta
     5761 aaacctataa aatttttaga tgaagcgaca agtggacaat taataaacta cttgaacatg
     5821 aaattatgga tcacctttca aatttagaaa atggaattct tatacttatt gcgcaacatt
     5881 tattgattgc aaaattatgt gacaaaaaaa actagtagaa gaaagaataa tggcttgata
     5941 ataaagtact acctattaaa ggtagtactt tatttaattt agtataaatt aacatactac
     6001 tatattttag gtttcatatt atataaatta ataatatact accatttgtt ggtagtataa
     6061 tttaaaggta ataagttatt cttttattac tatgtttggg ttttatagca aagaaagttt
     6121 aataagagtt tggacctagc aaacaatatt aatgaggtca ttaaatctta ttatctttag
     6181 tgatataacg aaatatctac acagacaaaa tgatagcgta aaatattttg tgtaaataga
     6241 tatttagggt agaaaaaaag aaagtccatt ctgtaaaatt aaagttacca caaccgaaat
     6301 tttataggag gactttctca tgcccaattt tactacagaa attatggaaa cactaatcaa
     6361 taaaggtgat ttagatgaat tatttcgatg tcatttagaa ttagctgtta attcattatt
     6421 gcaggctgaa ttaacagctt ttcttgatta tgaaaaatat gatcgtgctg ggttcaattc
     6481 aggtaattcc cgtaatggga attattcacg ttcattcaga acagagtatg gagaattaaa
     6541 tttggtaatt cctagagata gaaatggtaa gttttctcaa caaactttac ctgcttataa
     6601 aagaaccaat gactctttag aaaccactat tattcagcta tttcaaaaag gaattactat
     6661 gtctgaaatc tctgaattga ttgaaaaaat gtacgggcat cactatacgc cacaaaccat
     6721 ttcaaatatg actaaaatag tttctgaaga tattattgcc tttaaagaaa ggtccttaga
     6781 atccagatac tctgtcattt ttatggatgc tactcacatt cctttaaaaa gacaaactgt
     6841 ttcaaaagaa gcagtttata ttgtaattgg cattcgatta gatggaacca aagaggttct
     6901 tgggtttagt cttgctccaa ctgaatctgc ttatgtttgg aaagagattc ttcaagacct
     6961 aaaagaccgt ggtttaaaag aggttttatt agtcgtaact gatggtttaa gtggcattaa
     7021 cgatagtatt catagtgttt atccaaatgc tcaatttcaa caatgttgtg tccatatatc
     7081 tagaaatatc gctcataagg ttcgtgttag tgatcgacaa gaaatctgta gcgatttcaa
     7141 attagtttat caagcaagtt caaaagaaga agcaaataat caaataaggt ttatgataga
     7201 taaatggaaa aagcagtatc cacgagtggt taaattgctc atgaatcctg ctatattaac
     7261 cttttataat ttccctccgt caatcagaag aactatttat tcaactaatt tgattgaagg
     7321 ttttaacaaa caattaaaga aatataccaa aagaaaagag caatttccta atgaagaatc
     7381 tctggagaga tttctagtat ctcaattcaa caactataat caaaagtttc tatgccgagt
     7441 tcataaaggt tttaaagaaa tacatgatac attagaatca atgttttaac ttgatcaaca
     7501 gaatgggctt ttccatttac acaaaattat tgacgctccc gacaaaatta tgatcttgcg
     7561 taaagttata aaatattaat aaaaatattt tattaatgaa aagcttggta gcacggttat
     7621 tcatatgcta atttatttca ctttatagta aggggagttc ttagtatatt gttcaaactt
     7681 aaatttcata taatttaatt atgtaaatta gctattctaa gaacttctac ggataactgc
     7741 ccgtctaaaa tttaaataag agcttagaat cttcatataa gtaaggttaa tttttttgta
     7801 caaaaaagtc ggaatattct gagttactga aatgatataa aatcataaac taaatccctt
     7861 gattttattg actttcaaaa gtttatatat ataagttaat gattatggta aaatatatac
     7921 ataatcatta actcagaata tttttaagaa gggaaacaat agtgagctat aatgtacaac
     7981 cgttaaggac aaatgaagag atacaagatt ttcagttttg gctaagaaga acaagtaatc
     8041 cggaaagaga tagtttttta tttttgtttg gaatcaataa tgggttacgt atgtcagata
     8101 ttataggatt aaaagttgga gatatacgag gcaaaagtaa gcctattatt gttgaacata
     8161 aaacaggtaa gcgaaaaccg atttttatag ataatttgag agaagaaatt ttattatata
     8221 ctgaaggtaa agaagaaaat gattggttat ttccgagtcg gcaacaaggt cgacatataa
     8281 caagggatcg tgtctatcag atatatgcag atatagcaga aaaattagga cgagatgata
     8341 taggaacaca tacattaaga aaaacttttg ggtatcacta ctacaagaaa actagagaca
     8401 ttgctacctt aatgtttatt tttaatcata gctcgcaagc aattacaaaa aggtacattg
     8461 gtataactga agatgaaata ggagcatcac ttcgtggatt taaactaggt gtataattta
     8521 aaaattttta taaacagtaa agacaaagat taaaagctat ttttatctag aacgatttta
     8581 tttcataaag ttcgcggaat aaaagctggg gttactggta cttggtatag gttcgtgtta
     8641 tataaactaa gtaattaaat aacattttta ggttggaaaa ggatgaaatt aaaaaaagag
     8701 aatcaatacg gtctcaaaaa cgcaaacgct gcggaataag aaaaatactg ataactcaac
     8761 gtttagcaga ctatctaata gggatagtct gcttttttcg tttttgacca tacttttgat
     8821 cttctgcaaa cttccttgct tttcctctgt agaactcatg aaatctgcaa aacgaattgc
     8881 cgtttcctct gtctgctttt tcgcgatatg ggtgtatata tcatccatag tggttttaat
     8941 atctgtatgt ccaagtcgaa catacacttc tttgacagac gcaccagatt caaagagcag
     9001 actacaatgg gtatgacgaa aaccatgaag tgtgatatgt tttaagttat tctctttaat
     9061 gatgattttc aaattgtgat ttacatagtc taaataaagg ggcttatttt cttctgatga
     9121 aaacaaaagt tgttcaccat cttttatagg tttgattctc atttttagat attatgcccg
     9181 cacgtattca tcacatttct attgtgaatc gctttattcg accgacattt gacttttatc
     9241 ataatatact cggtttaaaa ttactaatga aaacaattaa tcaagacgat cataccatgt
     9301 atcacctgtt tttttcagat aatcaccacc gtgtgggaac tgaactgact ttttttgaag
     9361 tacaagaagg gaacaaccag ttttttggaa ccaatacgat tgaacgaacg atactaaaag
     9421 ttccttccga agcatcattg catttttggg aaatatattt tgaaacacaa ggcgtttgcc
     9481 attatggaat cgaatcattt tctggacgac ctatcttacg ttttgaaggg ccagatgcaa
     9541 cacgattagc tttagttccc ttacgcgaat ttgaagatat tgatgattat ttcccgtatg
     9601 tcacagacca gatccctaca gagcatgcca ttttaggaat tgatgccatt caactacgcg
     9661 tacaatattc cgatgcgact gaacgagagt tattgtcggt aggctggtag cacaaagaat
     9721 acgtcagttt cttttcgggt catcaacgag tgacaatctt agaaaatcaa gacactcagt
     9781 tctatcaaga agttcagatt attcaagatc gtgagaatcc agttgccatt gaagggattg
     9841 gtagcgtcca tcatgttgcg tttggcgtag aaaataaatc tgatttacaa cgcatcgaca
     9901 aacaattaca agagcgcaat tttataaatt caggaataaa agatcgtgaa ttttttgtat
     9961 cactttatta tcgtgaccca aatcaattat tgattgaaat tgctacaggg gaaggaaacc
    10021 tagatgcaaa agcgtatgaa aatcaaagtc cacgctttga agaaattcct ttatttttac
    10081 cgcagtactt aaatttcatt cgtgaacaag tggagcaata attagctaat caaatccatg
    10141 acgaagagta agaggttgac tcataagtca aaaaataaag aaaaacgcat caaatccatt
    10201 ctaaagaatg ttgatttgat gcggttccta tttttataaa ctatcatttt cgactaataa
    10261 aactacttat gagtcagcct ctcttttttt cttgattttt atacctcttt ctaggtaaac
    10321 taaacatctg ccaaaaatac cgtgtctcat tgaaacattc tcaactattt caaaataaag
    10381 gggagtacca aatgaacgag tatattgaaa ttatccacgc aagagaaaca acttaaaaat
    10441 gtcagtctca aaattcccaa aaataaaatt agtattttta ccagcgtatc tggttcagga
    10501 aaatcatcga ttgtttttga tactattacc caagaagctg gtcgtcaact gaatgacacc
    10561 tatgatagtt ttacccgttt atttttacct aaatacagtc gtccagaggt tgatgagatt
    10621 gataatcttt ctacggcgat tgtggtcaat caaaagaaga ttggtggcag tgcgcgctca
    10681 acgttaggga cgattactaa catcaatgcg ttatttcgtg tgttgttttc atgttttgca
    10741 acacctagtt taggatatgc aaatgcttat tcatttaatg atcctagtgg aatgtgttct
    10801 gtctgtcagg gaatcggtaa gacgattgca tttttaatag gattgcttta tttttcggct
    10861 attgataatg ctgattttgt attttctgat ccatttaccg cagaagtttt gtctggaatt
    10921 gaggaacagg caaagaatca tggttatttt gtattggtgc ataatgttaa ttcagcagaa
    10981 gatgttaaaa ctattcagag aaattggcgt tttgaggggt tcattgttgt tggtgtacgt
    11041 gtaaaagaat ttttggaatt aaataacgtt tttgatggtc ctgttgttta tattgatacg
    11101 catctttcaa aagagacatt aagagaactt gaacaacaaa tgaatcgttt atttgtaaat
    11161 acaaatgatt accaagctag tacattggca gtggaacatt tgattcatta tggtcatgag
    11221 aaaatagcgt ttctttcttt tgactacatt gtaggtgaac caagtgttat tcaagaacga
    11281 catcaagctt atatcgatac attaaagaaa tatcatttaa cctttaaatc gcattggata
    11341 tataacaact ctgaatttga gcgaatttat caagaattag ctgaatttac tgcaattgtt
    11401 gttactggag attatttagc ggctaaatta gtaaaatttt taaaaaataa gaataaacga
    11461 gtgatggaac aattatcgat tattggattt gatgatatta cttttgcaga attaatggat
    11521 cctgcgttga caacaatacg actaaatcca ggtaacaagg gggcgattgc ttttcaccaa
    11581 ctgattggac tggccaaaca agaagaaata aaaaatcaag tactactatt agagggagaa
    11641 ttgatttctc gagactctgt tcacaaaatc aaataaaaaa gctctgtgaa ataagtatag
    11701 ttaacaataa aatatccgaa cgatagagag aatgaactgt gatctagcag ttcctcgtcg
    11761 caaattcact agaattagca ttatttactc taaattgttc ggatattttt tcttgttttt
    11821 aattatgatt acagtgccta tttttaacga aatttgctta ttttcaaaat gtatgattat
    11881 atgttagtaa acactttcaa atatgaaaag aggagctatt atggtaggaa ttagggatgt
    11941 tgcaaaacgg gcgggtgtgt ccattgcaac agtatcgcgc gtgctaaatg aggatccgac
    12001 actttcagta accgatgaaa ccaaacgaaa gattgcagaa tcggttaatt tttatcatta
    12061 tgtcaaaaaa agtcccgttg caaaaaattt aacaaatatt gcattaataa cgacagtttc
    12121 tgaagtagat gagttggaag atccctattt tcgaacgatt cgcagaggga ttcaagtcga
    12181 agcagaacaa agcaaagtca atttgaagaa aattattcgt ttgtcagaaa caccgttgga
    12241 gcaagacgat ttgctaaaat gtcaaggtgt gttgattatt gggcaggtgc tatcttctgt
    12301 gattgaagaa gtatcagcaa ttaattcgaa tgtcgtagtg attgatgacc ctagtattga
    12361 agctgaagta gatgcagttt atactgattt aacaaaagca acgaatcagc atttagatcg
    12421 attgtataaa aaaggacacc gaaacattgc gtttatcgga ggaggacgtg ttcgtttaga
    12481 tagccaagga aataaacacg tgtcagatga tgattatcga aaaatagcct atgagacgtg
    12541 gatgaaacaa aaaggattag aaaaatatag tcaaatttat ttagaaggct ggaccacact
    12601 tgacggaatg caggcagcag atttactttt gaaaaaacat gctaaagata agttgccgac
    12661 tgctatcatg gtaggaaatg atcctattgc tgtgggggtt tatcgttcac ttcaaaaaaa
    12721 tggtctacat attccagaag atatttcgat tgtgagtttt gataatattg aagttgctga
    12781 atttttaaca cctgcattaa atacggtgaa tgtgaataca gaagaaattg gaaaactagc
    12841 agttcgcatg gttaatgaac gaataaaaga agttagaaat gtttcgatca aagtgattgt
    12901 ttctaataat attattgtta gagaaagtga aaaaaatatc gattaaaaaa gtaaacatcc
    12961 aaaaaagggt gtttactttt ttgtttacta atatttttag taaaaaatct cgacaaaaca
    13021 ttaagtaaac agttatggtg ttgttaacaa aaagaaaacg cattcaaaac aggaggaatt
    13081 aagatgaaac atttcaaaaa aatagcttta acagggatag ctgttttaac tgggacatta
    13141 ctcgcagcat gtggtggggg agatagtaat tctaaagacg ggcaagtaac tttaagctat
    13201 gcgagttgag atactgacca agctccagga ctaagaaaaa tgttagatga atttgaaaaa
    13261 caaaatcctg atatcaaagt agaaatggag acaacgcctt gggatcaata ttgggttaaa
    13321 ttagaagctg caacaacggg aggtaatatg ccagatgtag taaccatgca ctctagcgaa
    13381 tcctataaat atatgtcaca gaatgctttg atgggacttg atgatgttat caaagaaaat
    13441 gatattgaca tgggaaactt tactgaagga attgctgatt tctatacgct tgaagacgaa
    13501 ctatacgcaa tccctaaaga tgcttctgta gtggggctat ggtacaacaa agaattattt
    13561 gataatgcag gtgtcgatta tccagatgaa acatggacat gggaaacact cagacaagct
    13621 gcaattgatt taacagatga atcaaaaggc atctatggtt ttgcatcaca tattggaaca
    13681 gagactggct actggccatt tatttatcaa aatgggggag aagtatttgc cgatgacaat
    13741 acaaaatcag gtttgaatac cccagaagca caagaagctt tagcttttta tcgagatatt
    13801 gcacttgttg atcatgcttc accaacaatt acagaattac aagatcctga taagttaagt
    13861 cgttttcaat caggtagatt agcaatgatg attgaaggaa actggtttgc accgggattt
    13921 ttgaaaaatg actatctagt tgaaaatgtt gcttctgccg tgttacctaa gggaaaagtt
    13981 aatgcaacaa ttacaaatgg acttggttgg gctgccagtg cgagtacaaa atacccagaa
    14041 gaagtgaaaa aactaatggc atttttagct agtgaggacg ccaataaaat ccaagcggaa
    14101 acgggggcat caatcccagc actaaaaggc tttgaagacg agtgggctgc ttcttcagat
    14161 acaattgatt tgagtgcttt tggaaaaatg ttggagtatg gagtccctcg tccatacaat
    14221 aaagatggat tacgagctga acaagcagaa gttcaaatta ttaaccgtat tctgtctggt
    14281 gaagtaactg ttgaagatgg aaccgctcaa attgaaaaag aagtcaatgc gatattgagc
    14341 ggaaattaaa ttagttaatt atacaatata caggcgaaag gattctattt cgtctgtata
    14401 ttaatggatg aataccaaaa agggggatat tatgaaaaat cgaatttttg gaaaatatcg
    14461 taaagatcaa gtgatatggg gctatatttt tatttttcca acggcattag gattaatttt
    14521 attaaatata tggccagcaa ttcaaacagc aattttaagt tttcaaaaaa cggtagggtt
    14581 cggtgatact gaatgggtcg gattaagaaa ttaccaaaaa atgtttgcag acggtgaagt
    14641 ttttcagtct ttattaaaca cattaattta tgcagtggtt tcgataccta gtattgttgt
    14701 attatccttg ttggtggcag ttttaatgaa tagaaaagtt aaaggattgt cgatttatcg
    14761 gacaatttat tttctaccag tcgttgctgc accatccgca gtcgcaatga tttggcgctg
    14821 gatgttcaat aatgattatg gactgattaa taacatgttg gctaaaatag gtctcgatgg
    14881 accagcatgg ttaacggatc catcgattgc gatttattct attatcattg taggagtttg
    14941 gagtgctatt ggttataaca tggtgttatt actagccggc ttataagaaa ttcctaaaga
    15001 ttattatgaa gcagcggaaa ttgatggtgc tagtccaatc cagcagtttt tcaacattac
    15061 gttaccatta gttacaccaa cattattttt tgtagttgta acaacagtta tcaatgcttt
    15121 ccaagtattt gatgttattt ttatgatgat tactcctaat agtaccgcca tgtttaaatc
    15181 acaatcatta gcctatttat tctataaaca ttcatttatt ttaaatgaca agggatatgg
    15241 ttcggcaatc gttatgttcc ttttagtgat tatattaatt attactgcaa ttcaaatgaa
    15301 gttgcagaaa aagtgggtaa actattaagg aggctatcaa attatgaaaa ataaattttt
    15361 ttcaaatgcg ccaatccata tcatgcttat tttaggtggt attgcaatgg cattaccttt
    15421 tatttggatg attttaactt cagggaaaac gttggttgaa tctacccaaa ttccacctac
    15481 aattttccct gagaaatttc agctaagtaa ctatcaacaa gtacttgatt tattaccatt
    15541 catggctttt tatatcaata catttttaat ggttgttgga cgagtgattg gctcggtttt
    15601 attttcagca atggctgcat atgcttgtgc gcgtctaagt tttccaggga aaaatatcct
    15661 tttcagctta gtacttattc aaatgatgat tcccggttcc atttttatta ctccacagta
    15721 tttattagca caaaaattgg gtattttaaa tacaattcca gcattaatat taccaggggt
    15781 agttagtgcg tttggtacgt ttttattaag acaatttttc ttaggtttac ctgatgaatt
    15841 ggaggaagcg gcctatttag aaggtgccaa tatttggacg atctttttca aaattatgtt
    15901 gccgcttgct aaatcaggat tggttgcact tggaatattc acagcgttgt ttgcatttaa
    15961 agatttaatg tggccattga ttgtcaatac ttcaattgat aagatgcctt tagctccggg
    16021 attagcaaac ttacaaggac aatatgccac gaacttccca caattaatgg caggttcgtt
    16081 attagcaatt tggccaatag ttgtgatatt catcgttttc caaagaaaat tcattgaagg
    16141 aattgctaca acaggtagta aattataaaa ctattaatta gtgattggag agtaagtaca
    16201 tgacaaaaat tacatttatt ggtgcaggaa gtactatttt tgcaaaaaac gtattaggcg
    16261 atgcgatgtt aactccaagt ctacaagatg cacatattgc attgtacgat attgatgaaa
    16321 aacgactaaa agaatcaaat ttaatgctta aaaatattaa taaaaattcg aatgaaaatc
    16381 gtgctgatat tgtttcttat tatgatcgca aagaagcgtt aagagaggct gattttgtta
    16441 tcaatgctat tcaagtgggt ggttataaac cttcaacagt gattgatttt gatattccta
    16501 aaaaatatgg gttgcgacaa actattggtg acaccatggg gattggtggg atttttagaa
    16561 ccctacggac actaccagta atgttagact ttgctaaaga aatggaagaa gtttgtccaa
    16621 atgcttggtt attgaactat acaaatccga tggcgatgtt aaccatgggt gttttaaaag
    16681 cgacgagtat taagactgtt ggtttatgtc atagcgttca agtatgcgta ccagaattgt
    16741 ttgagcattt aggaatagcc gatcaatatg acttaaatga ctttcaatgg aaaattgcag
    16801 gcattaatca tatggcattc ttgttggaaa ttacaaaaaa tggggaagat ttctatccaa
    16861 caattcgtca gttagctgca gaaaaaccaa atccacatcc agattctgta cgttttgaat
    16921 tgttgaaacg ttttggttac tatattacag aatcaagtga acacaatgca gaataccatc
    16981 catactttat taaatcaaaa tacccagaat tgattgacga attagggatt ccgttggatg
    17041 aatacttacg tcgttgtgaa caacaaatta atgattggga aagcatgcga gaggacattg
    17101 taaataatgg ggagttaaca catacgagaa gccgtgaata tgcttcatat atcatggatt
    17161 ctattacgac aggaactccg tcagtgattg caggaaatgt cttaaataaa ggcttaatca
    17221 caaacttacc gacagatgtt tgtgtggaag taccttgttt agtcgataaa aatggcgtgc
    17281 aaccaaccta tgtcggtgaa ttgccaacac aattagctgc tttaaatcgg acgaatatca
    17341 atgttcaaga actgacagta gaagccgctt taacactaga taaatcaaaa atttatcaag
    17401 cagcgtattt agatccacat ctatcatctg aattgtcttt aacagatatc aaaaatttag
    17461 tggatgattt aatcgaagca catggggact atttacctga atataattaa aaagaagctg
    17521 aaagagtaag gaattttcaa ttccttactc ttttcttagt taaatttagt aaaaatattg
    17581 acaaaacact tagtaaacag ttacattagt gttaacaaaa agaaaacgca ttcaaattgt
    17641 ataaggagga gcataaaatg aagtggtcta aaaaaattat tttagcttgt atagcactag
    17701 gagcaacgat ctctttgact gcttgtggaa aaaatcagga agatgcaagc ggtaaggtag
    17761 aaatagagtt ttttaatcaa aaaaaagaaa tgactcaaac gattcaggag attgctaaag
    17821 attttgaagc aaaaaaccct gatattcatg taaaagttgt ggatgtaccg aatgctggtg
    17881 aagtaattaa aacacgtatg cttgcaggcg atgtaccaga tgtcattaat ctttatcctc
    17941 aaagtataga attgaaagaa tgggcgaaag ctggttattt agaggactta accgaagaac
    18001 cgtatttaga gaatatcaaa aatggatatg cacaacgttt tgctattgag gatcgtgtat
    18061 atagcatacc attaaccgct aatgtctatg gtttttacta taacaaaact gcttttgagg
    18121 aaatggggat tcaagcacca gaaacttggg cagaatttga aaaaatagta gctgagatta
    18181 aagatcaaaa caaagtaccg tttgcgattg caggagcaga aggctggact ttaaatggtt
    18241 atcaccaact agctatagcg actgctgctg gtggtgaaga ggaagcgaac aatgtttggc
    18301 gtttctcaga agttaatgga attaatgctg aaagcaaaga aatgcaaaaa gattttaatc
    18361 gattagattt gttaagagaa ccaaaagctt tgcaaaacaa ctggcaaggt gccggttata
    18421 acgatacagt agttactttt acaaaaggtg atgcgttgat tatgccgaat ggttcatggg
    18481 caatgccgat gattcattca caaaatccag attttgaagt aggaactttt ccttttccag
    18541 cagatgaagc aggtcaaagt ctaacgattg gcgcaggaga cctagcctta tcagtttctg
    18601 caacttctca acataaagaa gaagccaaac gttttgtaga atatatgaca acacctgaag
    18661 cgatgcagaa atattatgat gtagatggtg caccgtgtgc tgttgagggc gttatcgaga
    18721 atactcagga ttcaccatta agtggtttaa ctgaacttgc ttttactgat cgtcatttag
    18781 tttggttagc caaagattgg aatagtgaaa atgattttta taccttaaca acaaattatt
    18841 tacacaatgg caatcaaaaa gccatgatta acgcattaaa tgctttcttt aatccaatga
    18901 aagctgacgg aaggtaggaa aataaaatga aaataaaaga aattttaaat cgttactggg
    18961 caatcatttt tgtagcaatt ccaattattt tgcagatgat ttttttctac tttccaatgg
    19021 ttcaaggtgc tttttacagt ttaactaatt ggactggtct gacttataat tttgattttg
    19081 tgggtttgaa caattataag attctttttt ctgatccgaa gtttatcaaa tcgattggct
    19141 tcactttaat tgtgacgctt tgcatgattg ctggacaaat tggttttggt attttgattg
    19201 cgcgggcgtt gaacgcaaat attaaaggga aaacattctt tagagcatgg ttcttttttc
    19261 cagcggtctt atcgggcgtg gttgtttctt tagtatttaa acaaattttt aactatggtt
    19321 taccaaaaat tggtagtatg ttaggcattg aatttttaca aaccagtctt ttaggaacaa
    19381 catggggagc gatttttgca gtaatttttg ttttactttg gcaaggaatt gccatgccag
    19441 tcatcatttt cttagcgggt tttcaaagta ttccacaaga aattattgaa gctgctagaa
    19501 tggatggggc gaatgataag caaatttttt ggaatattga aatcacctac ttattaccaa
    19561 gtatttcaat ggtatttatt atggcattaa aaggtggctt aacagcattt gatcaaattt
    19621 ttgctttaac tggtggtgga ccaagcgatg ccacaacatc tttggggtta cttgtttata
    19681 attatgcttt taaaaacaat cagtttggtt atgcgaatgc tattgcttta gtcttattta
    19741 tcttaattgg gattgtttca ctggcacaat taaaattatc gaaaaaattt gaaatctagg
    19801 aggtttccca atgaaaacat caaaagaaaa aaatatttct aaatacttgc ttttattatt
    19861 agggggcttc atgattttag ttcccttagt ttcaacgcta ttcggttcat ttaaaacaac
    19921 acccgatatt atgaataatt tctttcgttt tcctaatcca gcgactttgg aaaatttccg
    19981 aagattgtta gcggatggaa ttggtcatta cttttggaat tcggcaatca tcacaatcat
    20041 tgcagtagtt gctgtgacag caattattcc tatggctgct tattcactag caagaaaaat
    20101 gactcgtggg aaagcatacg gattgatgta tttattttta attttaggaa tttttgttcc
    20161 tttccaagta attatgattc cgattaccgt tatgatgagt aaaattggtt tagcaaatat
    20221 gtggggatta atcttactct atctcactta tgccgtacca cagacactat ttttatatgt
    20281 tggttttatt aaactaaatg tgcctatcag tctggatgag gctgctgaaa ttgatggggc
    20341 tagtcacttt acaacttatt tcaaaattgt tttcccaatg ttaaaaccaa tgcatgcgac
    20401 gactattatt cttaatgctc tatggttttg gaatgatttc atgctaccat tattaatttt
    20461 aaatcgtgat tctaatatgt ggacactacc attgtttcaa tataattata cgggacaata
    20521 ttttaacgac tatggtccaa gttttgcttc ctatacagta ggaattgtca cgattacact
    20581 agtttatctt gctttccaaa gaagtattat agctggaatg agcaatggag cagtgaaata
    20641 accaataaat tattctcatt ataaaggagt tcataaactt atgattatat ttgacaatca
    20701 cacacaaaca tttcatttac aaaataaaaa aattagctat ctattaggaa tcgaagaaaa
    20761 taattattta acgcatcttt attttggaaa aaagattacg cactattctg gtgggtatcg
    20821 ctacccccga acagatcgtt ccttctcacc caacaatgca aatgcgcaag atcgtttatt
    20881 atctttagac acattaccat tagaatttcc aggatatgga catggtgatt ttagagaacc
    20941 ggcgcataat attaaattag gaaacggtag tcgtgtaaat gattttcgtt atgatagcta
    21001 cgaaattcaa aaaggaaagc ccagcttagc gggattgcca gccttatata cggaagatga
    21061 tgatgaagct gaaacgctga ttattcaatt gaaagacgcg attagtggat tgactttaca
    21121 gttgttctac acaatttatc aaaatcacag tgtaattatt cgttctacgc aattagtaaa
    21181 taatagtgat gaaacagtgg agattaaccg cttagctagt caatcaattg attttccaaa
    21241 tcgtccactc gagttaattc atttaaatgg tacctgggca agagaacgtc aaatgactag
    21301 agaaaaaatt catacaggga taaaagtatt agatagtaaa cgcggatcaa gcagccatca
    21361 acaaaatccg tttgttgctt tagtagatgc caaaacagat gaatttcaag gagaggcata
    21421 tggatttaat ttagtttata gcggtaatca cgaaacggtg attcaaaaag atccatttga
    21481 ccaaacacgg gtcattatgg gaattaattc atttaatttt ggttggcaac taaaaactgg
    21541 cgactcattt caaacgccag aagtcgtgat ggtttatagt aatgaaggtc tgaatacgtt
    21601 atcacagacg tatcatgaat tgtataatca ccgtttaatt cgtggaaact atcgtttaaa
    21661 agaacgtccc acgttaatca acaactggga agcaacgtat tttgatttta acgaagaaaa
    21721 aatcatgaat attgtcgatg atgcgaagaa cttgggcatt gaaatgtttg ttttagatga
    21781 tggttggttt ggcggtcgtg aaaatgactt cacttcatta ggtgattggg ttgaaacaga
    21841 aggcaaactt gcttgtggtt tggaaaattt agccaagcaa gtgcatgata acgggatgaa
    21901 atttgggatt tggtttgaac cggaaatgat ttctgaagat agtgaactat ttcgagcaca
    21961 tccagattgg gcattgggta ttccaggtcg tgaccgctca attagtcgaa atcaatatgt
    22021 tcttgatttt tcaagacaag atgttcgtga taatatctat caacaaatga cggcgatttt
    22081 tgatcgtgta ccgattgatt atgtgaagtg ggatatgaat cgaaatatga cagaagtcta
    22141 ttctgtcttg ttggactcaa cgatgcaagg tgaagtggct catcgttata tgttagggtt
    22201 atatgaattt ttagaaaagt taacaacgaa ttatccaact attttatttg aaagttgttc
    22261 gggtggaggc ggtcgttttg atgcaggttt cttgtattac atgccacaaa cttggacgag
    22321 cgacaatacc gatgccgtgg ctcgtctgaa aattcaatat ggaaccagct tagtgtatcc
    22381 aatttcaagt atgggtgcgc atgtttcagc agtcccaaat catcaaacag gaagagaaac
    22441 gagtttaact attcgtggca atgctgcgat ggctggtgtt tttggttatg agctagatct
    22501 aacgcaatta acttctgaag aaaaagcaga aattaaagaa caagttgcct tttataaaaa
    22561 gcatcgccaa cttttgcaat atggaacttt ttatcgcttg gaaagtccgt ttgaaggcaa
    22621 tgatactgca tggatgtttg tctcaaaaga ccaaacagaa gccatggtct tttatttccg
    22681 agtattagca gaagctgcgc atccattagt cacattgaaa ttagcaggat tagcagaaga
    22741 agcaacttat caaatggaag atgtaacatt tgctggcgat gaattgatga atctcggttt
    22801 ttatattgac tcagaactac atggtgatta tgcgacacaa cgcttttatt tgaaacagat
    22861 atagtagctt gaatttacaa tataagtgca acaccaaaag agactcattc caaacgaaga
    22921 aaatatgttt tatttacacg catttactaa aaagcagcct gatttgaatt gggaaaatga
    22981 agaagtacgt gaagaaatct atgcaatgat taactattgg ttagataaag gactaggcgg
    23041 atttagaatc gatgcgatat taaacatcaa aaaaaaacta gagtatggca tctttgaatc
    23101 tgatggagaa gatgggttag ccttcattgg gccctggatt cttaatcagt cgggcattga
    23161 agtgtggtta aaagaaatga gagagcggac atttaaacca cataatagta tgacggtagc
    23221 agaagccgat gttcctaatg aacgtttaga tgaatatatt ggtgaagatg gctattattc
    23281 gatggttttt gattttagtt atacggatat tgatgtacct gaaactggtg aatggtttaa
    23341 agatagccaa tggacatgga cggatatgcg taccaatatt tttacgaatc aattagttac
    23401 gcaagataaa ggttggggcg ccttatattt agaaaaccac gatcaaccac gttcaattaa
    23461 taaatatatt ccagaagaat ggatcaatga ttatagtaaa aaaatgttgg cgacgctttt
    23521 tatgttacta agaggtaccc cttttattta tcaaggacaa gaactaggaa tgaccaacat
    23581 agaaatggca tcattagaag attttgatga tgtagctacg cattctcagt ataaacgggc
    23641 attagaatat ggattggcac cagagcaagc attgaaagct gtcaataaac gaagtcggga
    23701 taattctcgc acacctatgc aatggaatac gcaaaaaaat gccagattct caacttctga
    23761 taatgtatgg ttaaaagtaa atcctaatta tccagaactc aatgcagaag ctcagttaac
    23821 caatcaacag tcggtattca attattatca tcaattaatt aaattaagaa aaaatagtgt
    23881 atacaaagac gtgctaatct acggacaatt tctaccagtg aaagataaaa atgaacatat
    23941 tttgctttat gaacgccaat tagaagataa aatcgtatta gttatcttaa acgttacagc
    24001 aacagaacaa gaatattatg tagatgaaca gtaccaacaa gtattaatca ataattatga
    24061 agatgtttta ttagatcaaa gtcgaaaatt gcgtttacga ccgtttgaaa gtattgtatt
    24121 agctaattat ggaggacaat tatgaaaatt aaaaacgaag caatgctcat cacctatgca
    24181 gatagtttag gaaaaaacat gggcgaatta aaacaagtct tagacaaaca tttacaaggt
    24241 gttattggtg gtgtacatct gttgcctttt tttccatcga caggtgatcg aggctttgcg
    24301 ccgagtgatt atacgacagt tgatccagct cttggaacat gggaagatgt ggaagcatta
    24361 ggtgaagatt attatttgat gtttgatttt atgattaatc acatttctcg tgaatcaaaa
    24421 tttttccaag atttcaaaaa aaaccatgag cagtcaccat acaaagaaat gtttatccgt
    24481 attcatgaat ttttcccaga aaatcgacca acacaagaag atattgattt aatctacaag
    24541 cgtaaagaca aagcgccgtt tcaaacggta gaatttgcgg atggtacgac agaagaggtt
    24601 tggaatacct ttggggaaga acaaattgat ttagatgtga ccaaagaagt tgtgaaagaa
    24661 tttattcgag aaaccatcaa agatatggcg aaccatggct gttctttaat tcgattagat
    24721 gcgtttgctt atgcgattaa aaaattagat accaatgatt tcttcgtgga accagatatt
    24781 tgggaacttt tagacgaagt gcgtcaagaa gcagcgaaat atgacgtcga attattacca
    24841 gaaatccatg agcattattc cattcaaatg aaaattgcca atcatgatta ttatatctat
    24901 gacttcgcct tgccaatggt cactctgtat tcactttata gtggaaaatc agaacgttta
    24961 gccaattggt tgaaaatgag tccgatgaag cagtttacaa cattggatac gcatgatgga
    25021 attggtgttg tggacgctcg tgatttatta acagatgaag aattggatta cacctctgag
    25081 gaattgtata aagtcggggc gaatgtgaag aaggtctatt caagtgccaa ttacaataat
    25141 ttagatattt atcaaattaa cagcacctat tatagtgcat taggcgataa tgaccgtagc
    25201 tatctgttag cacgtgcgat tcaagtattt gcgccaggga ttccacaaat ttattacgtt
    25261 gggttattag caggaaaaaa tgatattgaa ctgttggaag caacgaaaga agggcgtaat
    25321 attaatcgtc attactatga tttagccgaa attgaagaag aagtgcaacg gccagtgatt
    25381 caacaattat ttgcactttt aaaatttaga aatgaatctg cggcgtttga tttggatgga
    25441 acgattgaag tagcaacgcc atcagaatca gaaatagtga ttacacgtaa aaataaaaac
    25501 ggtagtgata gtgcaacatt aacggctaac ttggatacga aagaatttat cattgagtct
    25561 aaagatataa aaattttcac tagtgttgca ttaaaatttt aacctattcc atagaacaaa
    25621 aaatccttta tactagcact tggatgactt atccaagacc aataaaaagg actcatacat
    25681 ggataagtat aaaacaaata cagcatttaa taaatggttt tctgccataa aattaggaaa
    25741 actttctgtc aactcccaaa aatgtatttt tgattacaat tcgtatcgta aaaaactgtc
    25801 ttttgaagct gtgctcaagc tctttctcta cgcagttaat gaagaaaaag aaagtttgcg
    25861 tgatttgagt acagctctta tcgaccgatc gctccaacta gaaaccgatg tgtcatcgat
    25921 tagtcatacc caattgtctc gagcatttaa tgcacttgaa cctaaagtgt tagaagaaat
    25981 ttttcaacaa ctgttagaaa aagttcaaca tcaaatcaaa ccaaccaagc gaaatcatct
    26041 gtatctgatt gattccagta cgttttcgct tagtttaaaa cgtcataagt gggcgacttt
    26101 ccgtcaaaca aaatcaggtg tcaagttaca cttaaactat tgttatatgg acgatactac
    26161 gatgtatcca actgatttta ccttaacaaa tgcccatgaa catgatctca atcaactacc
    26221 agtattggtg aatcaaccag aggcaactta cgtgtttgat cgtgggtatt aggactttga
    26281 aaagatggat cagatgcatt gggatggcta tttcttcgtc actcggatta aaaaaaacac
    26341 caaagttcac gtgatagata ctctggaaac atcgccagaa acagaaattc tacgagacga
    26401 gctggttcgt ttaggatcta aaacctatct aaccgccaat ttccgactag tcactgttca
    26461 agataagaac ggacgtgtgt ttcaatttat caccaatcga atggacgtct cttcaaaaga
    26521 aatctctgat atgtatcacg ctcgttggca aatcgaattg tttttcaagc acatcaaaca
    26581 acacatgacc attaaaacct tcttttctca aagtgaaaaa ggcgtgcaga atcaattgat
    26641 tttaacgatg atttccgctc tcctcacgtt tctcatcaaa ttggagacga aaacggaaaa
    26701 atcagtgttc caaatcaaac gattctttcg ttatttactg tttcagccct ttgaatgttg
    26761 tctagaaaaa ttaattccca catgagaaaa aagaaatttg gctcgttact tttggttttc
    26821 agttgaataa cagataaaaa attttctgga aaaagtcatc ctctgcgtca gcacagtttt
    26881 ttttgactta ttttcagagg aaatagtttt cagaatattc taaaatgttt tatgcaacac
    26941 tagtgatatt ttttagaatt agatttctaa tgtgcatttt ttgagtatta aggagcaagt
    27001 atacaaagat ttataaagct gattaaattt gaataaaaat atgcaggaac gctactattt
    27061 ttagtagtgt tcttttttat atttgaataa aaaatgtggt ggttaaatgg tggttataac
    27121 aaaaaatgaa ttaatgaaat taggttatgg gaagtatcag tctgagagaa tcatacgaca
    27181 ggcaaaagca attatggctt caaaaagcta tacctattac gaaaatagaa gattaggact
    27241 agtacctgtt gaatctgtag aagaagtttt aggtatgaag atagaggtag aggaggattt
    27301 tcagaatcat ggttaatttt aaaaaagata aaaatgggaa atatcatttt actgctagtt
    27361 tgggattcga caaacgaaca gggaaaagag tacaaaaaaa cgtagagggg ttaaaacagt
    27421 taaagaagaa aaaaagcgta taccgaactt gtttctaatt atggaaaaga agcagaaact
    27481 tctcaaagta ctatgttgtt tgaggagttt ttttataaat tattcctacc ttattatcaa
    27541 gggaaagtag aagaaagaac atataaaaat cggttatcta caatcaaaaa aacttttctt
    27601 atttctttaa aatggagtta agagaaatac gtccaattga cattcaaaag tggcaaaata
    27661 atttattgag caaatataaa aattcttatg taagaaacat gtatgggatt tttcagatgt
    27721 cattagatcg agcagttgtt ttgggactac ttgaaaaaaa tcctgcaaaa gttattggaa
    27781 atgtgaagaa aaggaaaact acagttgatt tttggacaaa agaagaattt acaaaagtta
    27841 tttcaacttt ttatattgat gatttgtacc aacattttgg ttttatttgt atgtggttac
    27901 tttttatgac cgttatgcgt ttgggagaag ccactgcatt aacttggaaa gatattgatt
    27961 ttgaaaataa aaacttgcgt gtgaataaaa cactctatta taagagagcc aaaacctatg
    28021 agtttgtaga acccgaaacg gcttctagta ttcgtacaat cgaactagac gacaatacaa
    28081 ttaccttttt aaaagcttgg aaggagcgtc aagtttcaat aggaggcatg tcgtttgtac
    28141 tttcttataa tggaacacct actcagaaat atacaatccg tcatattatg agaagacatg
    28201 caacgatcgt tggtgttcat ataatccgca tacacggatt aaggcatagt catgcttctt
    28261 tgcttatatc acaataaaaa gatgcacttc gaattaaaga aaggttaaaa catgctgatg
    28321 tacaaacgac tttaggaact tatggtcatt tgtatctaaa ttcattgaga gagataacaa
    28381 atgatcccac agattatatc caaactaatt tttctaaaat caaaccgaca cctatttacg
    28441 ataataaaaa ataaatgata aacaaaagta ggatcacacc aaaagaaaca cactagaaaa
    28501 actataatac aggcttttct agcgtgttgt ttcttatttc cgcctattta tacattatac
    28561 tcataaagca tttaatgcat cgatcgtagc cgcattgtct atatgggtat ataaagttgt
    28621 tgtattggta cttgaatgtc ccaattgttg ggcagttaat accaatgaat ttgtctgttg
    28681 gtagagtcta gtagctaatg tatgacgcag tttgtgaggg ctgacttgaa ttctaaagcc
    28741 ttttgaatat tttgctacca tccgttcgat tgcaataccg gatatacgct tggcttctcc
    28801 ttggtaaatt gctagaaata aagctttttc gttttctgtt actttatatc gttctttccg
    28861 aatagctaga taattagcta aatattccat tgcgaatgct gctatattga cagcatctct
    28921 tttatttcct tttctagtta ctaccacatt attcttatct aaagataggt catctaaatt
    28981 aatatttata acctcggaga gtctcaatcc agatcctaaa atcagtgcat tgatcgccag
    29041 gtctctttct acatctcgtt gataatagat caaagctcgt ttactcaaag tatttttata
    29101 ttcattggca atataatcta gataattttg tgtatcctta tctagaaata attttttctc
    29161 aatggcattg gcacgtgcag ctaacgtagt tttatctgtg atggttgcta cttgatccat
    29221 aggattattg tctaagtatg gttttcctag aggattattg gaacttttcg aaagatattt
    29281 aaatagaacc tttaatgccg tgatatttct attgatagta gaagtagata gacctaagtt
    29341 attattatct ttgagcttag tttttgttgt ctcttttttt acattttctt ttgtgcgacg
    29401 tttcaaatga gatttgtaca agtttaggtc ataggcagtt aactgtctta tcgaggtgat
    29461 agaagtatca ggaacgatag actcactcag aagccattca taaaagagct tataattttt
    29521 tagatattct aaagtggttc gaggtgattg atctttatcc tcacgatctt gaacaaaatc
    29581 ttttatataa tcgggcatat gaagtatgat tgtatctatt ttttgatata tttctgtatt
    29641 tttcatgtca ttcatccttt caataagtag gggagaagag tatctctata attggactat
    29701 accaattata gaatgttttg tcaattggta tgctctaaaa tttattaatt tgtttcaaaa
    29761 aaagcatata aacaggtata cgtttaataa tttaaaataa atggtataca taaataggga
    29821 aggaaataga agggagcaat ctaaattttt tgaattttct taattaatta tatagaaaga
    29881 acaatctttc tatagagtta gacatgagag ggactatgct tgatttaaaa tcaacaagcg
    29941 tatatactgt atatacaaag aaaaaggagg gagaagacta tggcaattaa agaaagaaaa
    30001 tttagggaag tggggaactc agtagtatta actatttcaa aggagtttct taaagaaaat
    30061 gggttaaatc ccggagatac ggttttttta gatgaagaaa aacttcgagc ggcaattaca
    30121 aaaaaacaag ccttatctga tgaaattgat ttagcaatat ctcaagcatt aactgaatat
    30181 gatactactt ttagagagct tgtcgataaa tgagtattca ttacctagaa gccaaagata
    30241 ttataaaaat gaatgtaatc caaataaaaa aatactctcc taatgaaccc cttggtgtaa
    30301 aggatgcgaa tgctttagaa atgtgtgtag ggcaacttcg agcctcagct tttggtgaag
    30361 aggtttatcc ttcgatttat gaaaaagcag ctatcctact tatacaattg ataaagaaac
    30421 atccttttca caatgcaaat aaacgtactg ctttcttagc tacttttgtc tttttgaaaa
    30481 tcaatggaca gcttttagct attgatcaaa aagatgctgt aaatttagcg gtatatattg
    30541 ctacgtatga taaggatttc gatcaactaa aatatgaagt gatcaacact ataaagacac
    30601 atacgcagcc catttaagga gttactgtat gtgtctttat agggttatta gctgctatag
    30661 cgaaataatt gaagtaaatt acaaatagct ataattcaaa tccaactata tagaaaacgt
    30721 aaattttcta tatagttgga ttaagataac gactatttat tttatcatat ttttactata
    30781 ccctatactt ttacttggta tatttatgaa taatcgcaaa tttaccccta tactactagt
    30841 ttgtaagtta gattaataaa aatactaaag ttatttgttc ttttattaaa tacacctaca
    30901 acttataaat tgatggtatt tagataacac gaaagaataa aaataacagc tgatagtaaa
    30961 gtttgttgac gattgctact caatcgaaag tgagttagtg cgtatgaact tgcttctgaa
    31021 aacttatgaa atgaaagacc ttttacatct tgcagaaatc gttactggga ttaaactaag
    31081 caataaaaac taatgttccc ctcattcttt gaacttctac tatttgattt atttattctg
    31141 ataaatgacg atttcacctt caggatcttc aaatcccgta tagaatttat ttaagatctc
    31201 ttcagtatca gtaataggat aggcgatagg ccacataccc tttgatggtt agaataaact
    31261 gctcaaaatc tgtatcgtat gttggcttta gcatattaac aaaggctatc tccaaattat
    31321 aaaccataag cgttgaatcg ttcttaacaa tttcaattct tcctaaccca aagtcagaag
    31381 atataattac cggacttgcc ttacccttca tgtgggttgt tgagattcca tatttaaagg
    31441 gaattgtcgg tactatcggc ggtctgaaaa acatcccaaa aataaaaaaa tctttcccct
    31501 gctagtctcc taagtccctt tgtagcggat atggcaccat aatctcattt tatcgaagtg
    31561 agtcatggtt tatctgttgg ccaaccaaaa ttgatacaat ctaacgatgc accctctacg
    31621 gcattctagg ggagtgcact tgcattcacc caataaaaac cggcagttag caaagaactt
    31681 atgtagtcgt atgttcttac tgttaacata caagaggtct cctttccaca attgcttagc
    31741 agtaaacggg aatttacaaa gctaatctct tccattttct tatctttgtt ctaaaagtcc
    31801 caaatggagc aactgtattt acatgaataa atttatatac ttcccataca gccgttttgg
    31861 ttgcctcgtc tgcccatttt cttttgtgtg cagtgaatag atcttcatca ctcattgaat
    31921 caatcatcat gttaatatca tcaatattac gattgagaat ttcctctaat tctttcaatg
    31981 gtagatgtgc ataggtattt gtaaaccact tatataactc tcctaactga ttccatttaa
    32041 attgatcaga aggcgtttta acagataatc catttctttc atcattttcc cattttagaa
    32101 ttaaattcgt ccaaccaact tgatatgcta aattttcagc aggtgttcta tccacttcct
    32161 caactctttt atccttcaat tcttcaggaa tagtttcaaa ttctaaaata tatttagtat
    32221 aagtcctatt tatttctttt tttaattctt ctttattttt atatgttctc attatattac
    32281 ctccataaat tcctaattgt ctcctttaat tcatctataa tttcatccac gatattttaa
    32341 tccgttctac aatgcatatc tgatatgcat tgactgtcta actcatatgt gaatgattta
    32401 taaaatatcc caacgtctca tctaaagaaa tgttgatcgt cagcaaggag ctaaaaaata
    32461 cgaatatatt tcttttgtag taaaatcgga ctggtttgca tatcattttc ttcgtaaata
    32521 tcaaaatcat gttaactact atcaattact aataaaaact accactttaa gcgttaagaa
    32581 ctgttaagtt tagttaagtg aggatagttc acaaaatcca ttgttaaact tcacgaaagt
    32641 ggtaaaactc aaaatgcttt atataggatt acagcatcgc tctttcttcg attgcgcgtt
    32701 gggtaaaaaa tattatgaag taaaaattga cgatgacacg ttccttacgg ctcatcagat
    32761 gaaacagcta caaaaacgaa atgatcaact cgaagaaaat ttaattctaa aaaagcgatt
    32821 gccatattca cgcctcgctc gacgaactat taaaggtcaa gatttacctg aagatcatcc
    32881 tttttaggaa attgaagctt tttatgttgc acaatgcatt tataatacta caatgatgtt
    32941 ttcaccagat attcttctag ggtgcggtgt aatgaaacag atacacttgc aaaagaaaat
    33001 tcaccgcgaa tttactaaaa ttatgaataa ttatgttaat cttccgaaat ttttttccaa
    33061 aattaaggga tcaagctggt gttatagatt gtttaaggtt ggcaaaaaaa taacttcttt
    33121 ttaaacaatt atagaaaacc gatactctgt taagttggtt gacacggtgt cgatttttat
    33181 gactttctta ttctgaaact tcactaacgg acagtaccta aaaagtctat agtatggacg
    33241 tttacgagta ctattttatc aaaaatgctg ctcatttcaa cttatatgtt ttagtgaact
    33301 acccccactt agc
//
LOCUS       DAZAID010000021        31238 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000021 DAZAID010000000
VERSION     DAZAID010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.021"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACG9BE_000951"
     CDS             147..1016
                     /locus_tag="ACG9BE_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3031716.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACG9BE_000952"
     CDS             1225..2250
                     /locus_tag="ACG9BE_000952"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN3031717.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACG9BE_000953"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACG9BE_000953"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFN3031718.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="ACG9BE_000954"
     CDS             3333..5036
                     /locus_tag="ACG9BE_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3031719.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACG9BE_000955"
     CDS             5038..7071
                     /locus_tag="ACG9BE_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFN3031720.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACG9BE_000956"
     CDS             7082..7522
                     /locus_tag="ACG9BE_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3031721.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACG9BE_000957"
     CDS             complement(7722..8849)
                     /locus_tag="ACG9BE_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3031722.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACG9BE_000958"
     CDS             complement(8852..9529)
                     /locus_tag="ACG9BE_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN3031723.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACG9BE_000959"
     CDS             9793..11559
                     /locus_tag="ACG9BE_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFN3031724.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACG9BE_000960"
     CDS             11574..12011
                     /locus_tag="ACG9BE_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3031725.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACG9BE_000961"
     CDS             12024..13175
                     /locus_tag="ACG9BE_000961"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFN3031726.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13237..13935)
                     /locus_tag="ACG9BE_000962"
     CDS             complement(13237..13935)
                     /locus_tag="ACG9BE_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFN3031727.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13928..15280)
                     /locus_tag="ACG9BE_000963"
     CDS             complement(13928..15280)
                     /locus_tag="ACG9BE_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFN3031728.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15503..16903)
                     /locus_tag="ACG9BE_000964"
     CDS             complement(15503..16903)
                     /locus_tag="ACG9BE_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFN3031729.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17064..17801
                     /locus_tag="ACG9BE_000965"
     CDS             17064..17801
                     /locus_tag="ACG9BE_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFN3031730.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17926..18405
                     /locus_tag="ACG9BE_000966"
     CDS             17926..18405
                     /locus_tag="ACG9BE_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFN3031731.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18402..19169
                     /locus_tag="ACG9BE_000967"
     CDS             18402..19169
                     /locus_tag="ACG9BE_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFN3031732.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19399..19968
                     /gene="lepB"
                     /locus_tag="ACG9BE_000968"
     CDS             19399..19968
                     /gene="lepB"
                     /locus_tag="ACG9BE_000968"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN3031733.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20072..20674)
                     /locus_tag="ACG9BE_000969"
     CDS             complement(20072..20674)
                     /locus_tag="ACG9BE_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFN3031734.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20780..21010
                     /locus_tag="ACG9BE_000970"
     CDS             20780..21010
                     /locus_tag="ACG9BE_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031735.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            21001..21249
                     /locus_tag="ACG9BE_000971"
     CDS             21001..21249
                     /locus_tag="ACG9BE_000971"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3031736.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21355..21939
                     /locus_tag="ACG9BE_000972"
     CDS             21355..21939
                     /locus_tag="ACG9BE_000972"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFN3031737.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21942..23015
                     /gene="prfA"
                     /locus_tag="ACG9BE_000973"
     CDS             21942..23015
                     /gene="prfA"
                     /locus_tag="ACG9BE_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFN3031738.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23008..23847
                     /gene="prmC"
                     /locus_tag="ACG9BE_000974"
     CDS             23008..23847
                     /gene="prmC"
                     /locus_tag="ACG9BE_000974"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFN3031739.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23840..24019
                     /locus_tag="ACG9BE_000975"
     CDS             23840..24019
                     /locus_tag="ACG9BE_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031740.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24041..25066
                     /locus_tag="ACG9BE_000976"
     CDS             24041..25066
                     /locus_tag="ACG9BE_000976"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFN3031741.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25257..26501
                     /gene="glyA"
                     /locus_tag="ACG9BE_000977"
     CDS             25257..26501
                     /gene="glyA"
                     /locus_tag="ACG9BE_000977"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFN3031742.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26654..27601
                     /locus_tag="ACG9BE_000978"
     CDS             26654..27601
                     /locus_tag="ACG9BE_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFN3031743.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27735..28364
                     /gene="upp"
                     /locus_tag="ACG9BE_000979"
     CDS             27735..28364
                     /gene="upp"
                     /locus_tag="ACG9BE_000979"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFN3031744.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28503..28745)
                     /locus_tag="ACG9BE_000980"
     CDS             complement(28503..28745)
                     /locus_tag="ACG9BE_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031745.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28866..28938
                     /locus_tag="ACG9BE_000981"
     tRNA            28866..28938
                     /locus_tag="ACG9BE_000981"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28899..28901,aa:Thr,seq:cgt)
     gene            complement(29057..29695)
                     /locus_tag="ACG9BE_000982"
     CDS             complement(29057..29695)
                     /locus_tag="ACG9BE_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFN3031746.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29818..30450)
                     /locus_tag="ACG9BE_000983"
     CDS             complement(29818..30450)
                     /locus_tag="ACG9BE_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN3031747.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30801..31100
                     /locus_tag="ACG9BE_000984"
     CDS             30801..31100
                     /locus_tag="ACG9BE_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN3031748.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10660 a         5450 c         6291 g         8837 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatcca gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttcgct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata
    13261 gggcagatcc aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc
    13321 caggttctca tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta
    13381 aatgacacct tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa
    13441 aggtcgttca ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt
    13501 ttcattgaat tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact
    13561 taacgtgtag tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta
    13621 gtagtgacct agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat
    13681 atagttgatc aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa
    13741 gtcttgacca ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat
    13801 actaacgatc tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa
    13861 aaggtttccg ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag
    13921 ttcataatta gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac
    13981 gaagctgcaa tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt
    14041 tgattttttc gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc
    14101 cggcaacttt tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg
    14161 ggatatccat atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc
    14221 cgtctgcgta attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa
    14281 tcatatcgat tacttgattg atacctacag gatttttgac aagtactaat gtacattttt
    14341 tcccattgat attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt
    14401 gtttgatttt atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat
    14461 tgtagacatt atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat
    14521 ctgctgacac attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt
    14581 tgaagtcaca atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat
    14641 gcaagatgtg atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt
    14701 cacgatcttc ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat
    14761 tgaagattgg tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt
    14821 ctacaatcat tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa
    14881 tatttgtaaa taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt
    14941 catctatttc taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag
    15001 tcgatacaat tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt
    15061 gctggcgcaa aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga
    15121 caacgacgac ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg
    15181 ctagtttacc tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg
    15241 tttttccaac tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga
    15301 tttgctgacg aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt
    15361 ttcttattct taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga
    15421 tttaacaaaa aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt
    15481 ccctcttacc tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc
    15541 ctagatctcg tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc
    15601 caataaacat cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc
    15661 ctaaagataa accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg
    15721 tttgttcttc tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca
    15781 taaagacaac gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga
    15841 tgttatcttc acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg
    15901 ttcgaatccc gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa
    15961 tgatcagtgt tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag
    16021 tcgtaatcga ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata
    16081 aatggtcctt ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag
    16141 ctaacacagc taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt
    16201 tgtataatag ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa
    16261 tcaaaaacat gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc
    16321 cagtgacgtc aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa
    16381 cggcatcacg ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc
    16441 caaaaagtaa ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac
    16501 cagacaaacg cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc
    16561 gtcgtttaga aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt
    16621 gaaataatat ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag
    16681 tcgatagtcc agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa
    16741 catggctatc tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca
    16801 tcaatatata ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct
    16861 gtcctcgccg ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac
    16921 aatagtcgtt tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca
    16981 gataacaata cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa
    17041 aaagaaggag gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa
    17101 atcaaaaaat aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag
    17161 aagtaaagcc taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg
    17221 aattcatgtc tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta
    17281 aaagtgagta tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga
    17341 aaactgacca tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa
    17401 tggttttaac aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct
    17461 tcaatatgcg attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg
    17521 gaagtgccct gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg
    17581 atatccaaaa gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct
    17641 atgaacgctc tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt
    17701 gcgtgttcgc tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag
    17761 cttacttaaa aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac
    17821 gtaagaaaaa aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag
    17881 taaagtaagg aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca
    17941 cctattttga agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt
    18001 attgtgaata gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca
    18061 acaccaacta cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc
    18121 actacgacaa gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca
    18181 actgacagct caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc
    18241 aatccaagta caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact
    18301 gtagatgata acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca
    18361 ggaaataccc aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc
    18421 ttatcaacgg ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc
    18481 gtggatatac gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac
    18541 tttggcaaaa ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg
    18601 tttattttac tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg
    18661 tcactcagct ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat
    18721 cgatagataa tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg
    18781 acgaagatta tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga
    18841 tcaaaacaaa tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc
    18901 cttattacta tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct
    18961 actcggacat ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac
    19021 ctgtcggatt gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc
    19081 cttatcgtct gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata
    19141 gtcgtagcca aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg
    19201 ataaaaacgt ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt
    19261 atataacgag aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt
    19321 gtagaataaa gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat
    19381 acaagtagga ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta
    19441 ttagcggcta ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat
    19501 tcgatggacc ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat
    19561 gaacgaatgg atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa
    19621 cggatcatcg gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat
    19681 ggcaaaaagt atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag
    19741 ttacaaggag aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc
    19801 tttacgagtg actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac
    19861 cgcttgattt caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga
    19921 aaagttgttt tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa
    19981 aagaagctgt gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct
    20041 tggagttgct cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa
    20101 gcgaagcgtt tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag
    20161 agacaaagcc cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga
    20221 ctttcggatg actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag
    20281 gagctaaaat ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta
    20341 caagtgatag aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt
    20401 ggatcgttcg gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca
    20461 tagtttctga agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa
    20521 caagcccagt tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat
    20581 aagtagttag ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat
    20641 ggttagcgat ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg
    20701 aaattttaac ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa
    20761 gggagaatct agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa
    20821 gtgtgtccga gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga
    20881 aaaatatatc gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata
    20941 tttaggcttt atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt
    21001 atgggaataa aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca
    21061 gaaggttacg cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac
    21121 tactctatat atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa
    21181 gtttttccaa atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa
    21241 caaaagtgag caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa
    21301 atgctgaaaa tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca
    21361 caactatttt ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc
    21421 gcacataatt atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca
    21481 agagatggtt taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata
    21541 tttgaagaaa cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg
    21601 ctgatcgatg aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg
    21661 gacgaattgg atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta
    21721 tttgaaggat caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc
    21781 tgttggttct gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt
    21841 tatgaaggcg atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa
    21901 cattactttc acccatcaat gattacagaa ggagaataag catgtacgat caattacaag
    21961 caattgaaga ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg
    22021 atacaaagcg ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag
    22081 tttatcgtcg ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg
    22141 aaaaactgga tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag
    22201 aaaaagaagt cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg
    22261 ataaaaatat catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg
    22321 ctggcgatct tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag
    22381 tgatggaagc taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg
    22441 gagacaacgt atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc
    22501 cttcaactga atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag
    22561 aagctgaaga agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg
    22621 cttcaggtgc tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact
    22681 taccaacagg gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa
    22741 aagcaatgaa aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg
    22801 agtatgatgc caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga
    22861 cgtataattt cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat
    22921 tagatcagat tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc
    22981 aaacatcaaa actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca
    23041 cgggcttctt cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt
    23101 ttggaacgaa aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca
    23161 gaagaagaac agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct
    23221 cagtatttgc taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca
    23281 ctgatccctc gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt
    23341 gtaccgttag aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa
    23401 gcagcaagaa aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta
    23461 gcgaaagaaa atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta
    23521 ggaccagtaa tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg
    23581 aatgaatggg aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt
    23641 gctgagaacg atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta
    23701 aaatcaaatg gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa
    23761 atatttcagc aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat
    23821 gaacgaatga tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt
    23881 tattataaaa aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt
    23941 ttatccacta ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag
    24001 acaaaataag aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga
    24061 tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc
    24121 taccgaaaca gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt
    24181 gttttctgta aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga
    24241 acaagtaaaa gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt
    24301 ctggcctgga ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt
    24361 gacgggagga ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt
    24421 aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag
    24481 tccaacgact gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga
    24541 tgatggagct acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc
    24601 catgcctatg attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga
    24661 atctcctgtt gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg
    24721 gatgaaatac aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg
    24781 gtcaactgca gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc
    24841 cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc
    24901 agtagaagcg gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact
    24961 tggattagat ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat
    25021 gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg
    25081 tcgcattctt ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa
    25141 ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg
    25201 aaaaataaaa ttgtaggaaa ataaaattgt aggaaaataa aaaggagctg ttgtttgtgg
    25261 tagattacaa aacgtttgac cctgatcttt gggctgcgat cgccaaagaa gaagaaagac
    25321 aagaacataa tttggagctg attgcctctg aaaactttgt ctccgaagca gtgatggctg
    25381 cacaaggaag tatcttgaca aataaatacg cagaaggtta tccagggcat cgctattatg
    25441 gtgggtgtga atttgtcgat atcgttgaaa atttagcgat tgaccgtgca aaagaattat
    25501 tcggagcgaa atttgcgaac gtccagcctc actccggctc tcaagccaac acggctgctt
    25561 atctcgcttt ggtagaacca ggggacacga tcttaggaat ggatctttcc gcaggcggac
    25621 atctaacaca tggatcacca gtcaacttca gtggcaaaac ctatcacttt gttgcttacg
    25681 gtgttgatcc gacaacagaa gttattgatt ataatgtggt acgtatttta gctagaaaac
    25741 atcaaccaaa attgatcgta gcaggtgcca gtgcttacgg acgaacgatc gactttgcga
    25801 aattcagaga aatcgcagat gaagtaggcg caaaactgat ggtagatatg gcacatatcg
    25861 caggattagt agcagcagga cttcatccta atccagttcc ttatgcagat atcacaacga
    25921 ctaccactca taaaacattg cgtggccctc gaggcggcat gattttgaca aacgatgaag
    25981 cattagcaaa aaaaataaat agtgcggtat ttcctggtat tcaaggcggc cctctagagc
    26041 atgtgattgc gggtaaagca gtagcattca aagaagcatt agatcctgca tttaaagaat
    26101 atagtgaaca aatcatcgcc aatgcaaaag caatggtcaa agttttcaat caagcaattg
    26161 gaaccagagt catttcaggg gcgacagata atcatctgat gttgatcgat gttcgcgagt
    26221 taggtataaa tggaaaagaa gcagagagca ttttggacag tgtgaatatc actgtaaata
    26281 aaaactcgat tccttttgaa acactaagtc cattcaaaac aagcggtatt cgaatcggca
    26341 cgccagcaat cactactcgc gggttcaaag aagaagatgc tgtaaaagtg gctgaactag
    26401 ttgtcaaagc attacaagca aaagatgata acgctcaact agacgaagtc aaaacaggtg
    26461 tacgcgaatt aactgaaaaa tttccgttgc ataaaaaata aaaatttaaa ttccattagt
    26521 aaacagaaaa gttcaatgtt gggcttttct gtttattttt tgggaaaacg gattctttga
    26581 caatatcggt gatgctctct agtatatgag gtgtaatttt agtcttcaca ctaaatctca
    26641 gggaggaaaa gtcatgactg atcgtatctc gttagaaaag gaagcagtag cattcagtga
    26701 tgcaaatgcg cctcatccgc gtatctatga actagcaccg gaagaaggaa gagttttact
    26761 agaagaagta caagaatcac ctgtgaacaa atattccgtc gatattgaag acttgatggt
    26821 ggatactggc gaatggggaa aactcaatat tcgtctcgta cgaccagaag ggaatatgga
    26881 taaattacca gttatttttt atattcatgg agcaggctgg gtttttggca gtgctcaaac
    26941 ccatgataaa ttgatcagag aacttgcggt tcgtacgaat tccgtcgttg tctttccaga
    27001 atatagtcga tcaccagaag caaaatatcc aacagcaatt gaacaaaatt atgctgtgtt
    27061 gcaacagctg aataaattag ccaagtcaaa aaatctggat ctcgagaatc taatcgttgc
    27121 aggagattca gtaggtggta acatggctac agtcatgacg atcttaacaa aagaacgaaa
    27181 gggcctccca atatccaagc aattgctgta ttatccagtc acaaacgctg attttgatac
    27241 agattcctat catgaattcg gagaaaacta ttttttgaca agagaaggaa tgatgtggtt
    27301 ttgggaccaa tatacgacga atgaagaaga gcgtgctgaa atcacagctt cgcctttgag
    27361 agcaaatata aatgaactaa atgatcttcc ttcagcaatg attttaacgg gagaagcgga
    27421 tgtattaaga gatgaaggtg aggcttatgc gcgcaaactt cgtgatgcag gggttcctgt
    27481 tacacaagta cgcttccaag gaatcatcca tgattttgtc atggtaaatg ctatggatca
    27541 gacaaaggca acgcgtgcag caatggcatt atctacttct tggatcaaga aaaaagaata
    27601 agctttgctt gtccgcagga aaacttcttt tcaaaaacgg attttcttag gaaaaatggc
    27661 gagtttgact agtcaatcac ttgattttgt tttacaatta aaaagaaacg acagaacaaa
    27721 ggagagctta aaaaatgggc aaatttcaag tgatcgatca tccattgatt caacacaaac
    27781 taactattat tcgggacaaa aactgcggaa caaaggtttt tcgtgaagta gtcgatgaaa
    27841 tcgctatgtt gatggcatat gaagtatcac gggatatgcc attggaagac atcgtgatcg
    27901 aaacaccgat cactgaaaca acacaaaaaa cgttgtctgg taaaaaagta gcgattattc
    27961 caatcttgcg cgcaggtatt ggaatggttg acggtatctt ggaattgatt ccagctgcta
    28021 aagtgggaca tatcggtctt tatcgagatg aagaaacatt ggaaccacat gaatatttcg
    28081 tcaaacttcc agaagacatt gatgcacgtc agttgtttgt agtagatcca atgttggcaa
    28141 caggcggatc agcaatcatg gcaatcgatg cattgaaaga acgtggcgcg tcgaacatta
    28201 aatttgtttg tttggtagca gcacctgagg gagtaaaagc tttgcaagaa gcgcatcctg
    28261 atattgacat ctatacagct tctctagatg aaaaattgaa tgaacatggt tatatcgttc
    28321 ctggactagg ggatgcgggt gaccgtttgt ttggtacaaa ataagtaaat acgaaaaagg
    28381 gtgtgacaca acttttgtca catccttttt tggtcatttt taacgtttga tgcagaacag
    28441 ctttttcaca gctttcgttt gcatataaaa aaaccatccc aaaaatgcag agatggtcaa
    28501 attcaaatct tttttagttc tttgtagtta acgattacta cacccttact ttgcgtcatc
    28561 aagttcgcat tttctgcagt ttcatcaatc tcaacaacag cggaattttt caattgcttt
    28621 gaaatcgttc cagtttgcga cttgccgtga attgtgaaca ttacgtgagt attgatcttg
    28681 aatttttcac caatttccga tggtacgata gttgtatcaa attttccaaa agttgccatt
    28741 ttcataaaac ctgcctttca catcaattat accacgtttt atgaaaaata tcatgaaatt
    28801 aaactaattt tccatcggac tctttacaat cagccaaata gagtatataa tttcttttgg
    28861 ataaggccag cttagctcag ttggtagagc aacgcactcg taacgcgtag gtcgtaggtt
    28921 cgatccctat agctggcatt aaaagagaag acagatatat caaggatttt taatccattg
    28981 atacatctgt ctttttgttt aaaggaaata aagtatgctt tcttagcaat agagcatgta
    29041 ttatttctat aaaaatttag gagaataaaa cgactagcaa tatagctgtg aaatcaaata
    29101 tcacatgaac tgcccaactt gtaatgatcg agttgctttt aatggcagct tggttgaata
    29161 acaatcttgc caccccttgt atcaaaagga tgtgtgctac agtgtgtaaa atatttccat
    29221 tatcataagt actgtaatgg ataaatccaa agatgattgc tgacaagaaa ctagcgacag
    29281 gaatgctgaa cttactagaa aaaatagcga gaaaatacac tgagaacaat tcttctccaa
    29341 gtatcatgat aggcaaaata ataaataata acgggctgaa agcctcattt attggattgc
    29401 ctgttaaacc ccatttcaaa atttgttgca aaaataccga gactgcggca ctgattacta
    29461 cattgataaa aaaatatttg gctatatttt tgacaggagc gacaggtttc gagaacattt
    29521 ttttcgtatc ttttttacca aaaacaagtg taaatgctat aaatccagtg atagttaaag
    29581 aaaaaatgct aataatggat ggcgcaaata gcaaccctaa caaaccgagt acaacgacaa
    29641 tagtgcccaa tatccctttt ttaagttcta ttttttttcc taaaatgtta ttcatgataa
    29701 taactcctac tattcagttt ttgaagatag agtcagtata catgaagagt taaaataggc
    29761 aatgtttttt tatggatatt tgtattgcta aaaatttatc ttatatattc attttgatta
    29821 tactactttt atttgtaaaa attttctagc aaaaccaagt gcttgcaact gattaagcgt
    29881 acaatttttt agatgttccg agcgtacttc aatcatttca aaggagttgc tacttaaatc
    29941 gatatttttt aaatcagtat caaaaaagct agcattttct aaatcattag tagaaaacat
    30001 tattttagtg agggaacagt ctacaaaatc tgcgcttgat aagacagagt gctcaaaggt
    30061 actttctttt atgtttgaac cagataaatt tacatagtcc attttacaat tctcgaataa
    30121 cgtgttccca atataacttt ccatcaattt agtacctgtt aatttacaat tgacaaattt
    30181 cacgcgatgc aacatgcttt ttgtcaatat acagcctgaa aaatcacagt tttcgaatat
    30241 acagtcggta atctccattc tttgaaaatc attggaagca aaaaatgcat acttgaaaat
    30301 agatttgtaa ataattggat ggcagattat ttggttttcg atgacctctt tttgtgaaag
    30361 gatattatac agttcaaaat cttcgttttc atagatatga aaaaaggatc ctttattcaa
    30421 gcttgcttca ttaactttag gaaaaatcat atttaattac ctccattttc ataaagtcat
    30481 attaacaaaa gctaaaccaa cttacattag cagaaaatga gataggtttt cgcctttttc
    30541 gataacgata gtgtcgctat ctcttgaaac gttcttagaa ttgccctgag agacacaaat
    30601 taggcgtaat tctaataaaa atcttagaat aaaaattttt actgatttaa tttagaatgt
    30661 ttttaaaata aaaatcgttg ccataacagt gtattgaaag gttttaacaa tccagtaatg
    30721 atttaaaaaa atctagatgt agcgtatact ttgtttatag aatgtagtgg gaagaaatat
    30781 atttttggga ggaaatcgtg atgtctaaaa gtgaagaaat agaaaacatg gtactaaaat
    30841 tatatgatct cgttttaaat ccagagatca aagaaaagga acgtatactt ttgatagacg
    30901 caaagacagg cttagaaaaa ggtcagtatt atcctaaagt cataaataat ttggaacgta
    30961 gtttacgtcc gttagcaatc aggggagaat tgtctaaacc agtatcaccg ttttatatgg
    31021 agatatcaac aataggaaaa tttgaaaaag aattaggtcg aggaatggct tcggcaccga
    31081 ttacttttgg gcatttataa aaacgagtta ttcatctcaa tgaattaaaa agtatagtca
    31141 atctcataat gcaggctagg ttcatttatt tgaacagagt ctgtattttt ttattctaaa
    31201 taaagaaaca gatgtttctt tatattgtat tttatcct
//
LOCUS       DAZAID010000022        30899 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000022 DAZAID010000000
VERSION     DAZAID010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.022"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG9BE_000985"
     CDS             complement(296..1024)
                     /locus_tag="ACG9BE_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3031749.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG9BE_000986"
     CDS             complement(1002..2576)
                     /locus_tag="ACG9BE_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN3031750.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG9BE_000987"
     CDS             2805..3743
                     /locus_tag="ACG9BE_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3031751.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG9BE_000988"
     CDS             3755..4666
                     /locus_tag="ACG9BE_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3031752.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG9BE_000989"
     CDS             4690..6162
                     /locus_tag="ACG9BE_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN3031753.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACG9BE_000990"
     CDS             6213..6824
                     /locus_tag="ACG9BE_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN3031754.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACG9BE_000991"
     CDS             6854..8767
                     /locus_tag="ACG9BE_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFN3031755.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACG9BE_000992"
     CDS             8895..11876
                     /locus_tag="ACG9BE_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFN3031756.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACG9BE_000993"
     CDS             11873..13534
                     /locus_tag="ACG9BE_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFN3031757.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACG9BE_000994"
     CDS             13574..14506
                     /locus_tag="ACG9BE_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFN3031758.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14898..16445)
                     /locus_tag="ACG9BE_000995"
     CDS             complement(14898..16445)
                     /locus_tag="ACG9BE_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFN3031759.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="ACG9BE_000996"
     CDS             complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="ACG9BE_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFN3031760.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(16890..17084)
                     /locus_tag="ACG9BE_000997"
     CDS             complement(16890..17084)
                     /locus_tag="ACG9BE_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031761.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(17310..18512)
                     /locus_tag="ACG9BE_000998"
     CDS             complement(17310..18512)
                     /locus_tag="ACG9BE_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3031762.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(18515..18709)
                     /locus_tag="ACG9BE_000999"
     CDS             complement(18515..18709)
                     /locus_tag="ACG9BE_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN3031763.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(18756..19604)
                     /locus_tag="ACG9BE_001000"
     CDS             complement(18756..19604)
                     /locus_tag="ACG9BE_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFN3031764.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(19809..20159)
                     /locus_tag="ACG9BE_001001"
     CDS             complement(19809..20159)
                     /locus_tag="ACG9BE_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031765.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            20337..21200
                     /locus_tag="ACG9BE_001002"
     CDS             20337..21200
                     /locus_tag="ACG9BE_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3031766.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            21824..22816
                     /locus_tag="ACG9BE_001003"
     CDS             21824..22816
                     /locus_tag="ACG9BE_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3031767.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            22832..23716
                     /locus_tag="ACG9BE_001004"
     CDS             22832..23716
                     /locus_tag="ACG9BE_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3031768.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            23765..25372
                     /locus_tag="ACG9BE_001005"
     CDS             23765..25372
                     /locus_tag="ACG9BE_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3031769.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            25373..26857
                     /locus_tag="ACG9BE_001006"
     CDS             25373..26857
                     /locus_tag="ACG9BE_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFN3031770.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            27034..28026
                     /locus_tag="ACG9BE_001007"
     CDS             27034..28026
                     /locus_tag="ACG9BE_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3031771.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            28078..28947
                     /locus_tag="ACG9BE_001008"
     CDS             28078..28947
                     /locus_tag="ACG9BE_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN3031772.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(29367..>30899)
                     /gene="guaA"
                     /locus_tag="ACG9BE_001009"
     CDS             complement(29367..>30899)
                     /gene="guaA"
                     /locus_tag="ACG9BE_001009"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFN3031773.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         9690 a         5145 c         6234 g         9830 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
    14821 agtaagcgcc ccatagaaca gtacctgtac gtcaaataac ccgacctata gaatagaatc
    14881 gggaatgttt ggcgttttta gttacgagaa aaatgttgtt gtacatcctt gttccaaggc
    14941 aacagttgat ctaaagtttc tgctgaaagt acgggaagat ttggtatctt atcaaataga
    15001 aactctagat actttacagg attcaatccg ttaagttctg ctgttttata tacactcaga
    15061 atgattcctg aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt tttccgtcct
    15121 atcaccagct ctttgatatt tcgctccgca cgattatttg tgagttctaa acgaccatct
    15181 tcaaaaatag gttcaaaacc acttcgtagt ttacacgcat attgaacggc tgcatccaac
    15241 ttggattttg caacaggatc tatcgttgcc atccactcat aaaaagcaat aaataatgga
    15301 cgcagttctt gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt ccatttcttt
    15361 tctaggacaa accatttatc caattgtttg actgcttgag ctgcgggaat atccgtgttt
    15421 ttctttcctt ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa acaatagaca
    15481 agtcgtacac tttccacggc attatacccg ctatagccgt cacagtgtaa ataccctgta
    15541 aactccttga gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg gtgataataa
    15601 acgatgggat gctgactgtc ttttcctgta caaaactgcc agtaataggt atctgttttg
    15661 gcactttcaa tcacacgata acttgtttca tccccgtgta gaatctcttc ttgattaaga
    15721 tagactctta atcgttctcc taaagggtct aacgcgtttt gcactgcttt aatatgccaa
    15781 ttggtgatat tatctctcga aatatccaac ccatgtgttt gtttccaata gttctcttgc
    15841 cgataggcag gaactttctg accaaatttc atccggatcg tttccgccac taaactattg
    15901 gaacctaagc tattagaaat caaaggttta ggaacgatcg cttttttaaa agaagaaagt
    15961 ccttcttttt cgcagctttt acaacaatag gtatgttgat aatgggtgag acaatccaac
    16021 atagcctgat ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc tttcatctgt
    16081 gaaccgcaat gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt ctctgttata
    16141 ggtaagtgtt tgattttgtc tgctttcgtt cctacaactc gttgatgtcc ttttactgga
    16201 ataatggtgg cagctgcttc tggaacactt acagaaattt cttcatcact aaaaagattc
    16261 aattgcccat tcgtttcctc aaagagagtc tctttagagc gtccaaataa tttgttggtt
    16321 aaataattga ccttttcatt caatagttgg atttcagttg taagattctg aatggtttgt
    16381 tgttgtgctt gattggtttt atgggcttct tgaagcaact gaagcaacgt atctgtagtt
    16441 tccaaaattt ttcaccacct tgaatcgaac tgattttgtt acagaatacc acaagaaacg
    16501 cacaagaaat aggggttcaa cacatttttt gtgttaaccc cccagcttaa aaaactcctt
    16561 tttttcctgg caaaatagct cgaggttgat cgatacttaa tccttctaac aaccatcgaa
    16621 cctgttggtt cgttaaggtt cgaacttcgt ctttcgtccg aggccatttt aacttgccgt
    16681 tttctatccg cttataacag agaagaaaac catcaccgtc ccaatagaga agtttgaaac
    16741 ggtcttgacg gttcccgcaa aataaaaaaa cggcatcttc gtaaagctcg agttgatact
    16801 cttgttgaat aagactggca agaccatcaa ttccttttcg caaatctgtt tttccgcaca
    16861 caatataaat atgtttggtt tgaagtaaat cataaagcat tggacaacag ttctttcaaa
    16921 atagcttgaa gaatataatt gttaataccg ttatagatca aaacttcaga accattctct
    16981 cttttgatgc gcatcgctaa cttggcagcc gcgggtttac ctttttctgg tgcactcttt
    17041 tcgtttccta attggacagg aacaatctca tttaatttat acatagaaaa cctccagact
    17101 tcttgatagc tcaagtatat cttgaggtgc atttgttagt aaggtactgt tctatggggc
    17161 gcttacgttg aaaaacgttg atttatcggc attccccatc catattatat ttacgtctta
    17221 ttcccactca atatgagtag cctcgtaaag aagctattat atcagtgctt tttcagccta
    17281 ttcctcattt tggtagggaa atggtagggt tagattttta ctgcttgatt tccattaaag
    17341 tttgcaactt tagattttgt tgcacaggta aatttaatgt ttcctgtcat ttgttctgca
    17401 attccttcat cattacggct ccatagatga gcgtagtgct ttaaagtgat ttcagggcta
    17461 gaatggccta accgttgcga cacgaccaga atgtctgcgt taaactcatt gattaggtag
    17521 ctaacatgag aatgacgtaa tccttttcct tgtattaggg gtaccttagc tagtttggca
    17581 taacgctgaa taattcgttg aacagttgaa cgatagagtg ggaggtcagt gtagctaaga
    17641 ataaattgtt ctacaccatg atttttttga ataactttcc acttttttag cacttcaatt
    17701 gtatcatcgt ccagagaaat gatccgcaag ccgctttcag tttttgtgta aggtttccga
    17761 ataaaatctt tttgatttgt catttccaag gtgtgatgaa ccctcaactt tttacgtttg
    17821 aagtccacgt cattccaatt aagtgctaat ccttcgctta cacgaacacc agtcatgtaa
    17881 tatagccaga tcattaaaaa acacatatgt tcgtagaaat catctttata aatttttgcc
    17941 aaaacttctt caaattcttc ttttgtccag taaggaacga ttgattttcc ttttggaatt
    18001 gcttttgttc ttttggaaat attctcattc agaaattgca gggtgaccgc atagtctaat
    18061 gatttacgaa acataccgta cataagtgaa ctgtatgctt gtgagtatcc gctatcgttc
    18121 aataaccaaa ttcgataatt ttcacaatct cgcacattga tttcacgaag cttcttatca
    18181 ccgaaaaatt cagtaatctg aatcaatcca cactcacgag aactccaagt acttttttgt
    18241 acgctagctt tgtagtgtgg aacgtaagac tgctccataa attctttgta agttaaatca
    18301 taattcgcaa atccatttac aaccatgtat tcatttttta tacgcatgac ttctttgtgt
    18361 gcttcacgag ctgatgagaa tcgttttcct agagaatctt ttgttccttt cttctggatc
    18421 ctctttccag tcacttgatc tgttcctaac tcaactagat aagaaaagcg accattttta
    18481 tcttgataca cacaagggta ttttgttttc atagttattc ctcctgtgaa agaggaacac
    18541 ctataatttc ttcaattact tgtcggggta ctaccccaac tcttttatta ttataatatg
    18601 gatatccctg atttaccatt cttgtttttg cttgtcggat tattgtggta gcttgatgtg
    18661 ttttaaatcc tagagcctct aaatcttttc gacaaattgt atttttcatt gtataaactc
    18721 actttcaaag aaaacactat cattgggtaa tagtgttatt tctttatttt tgtataatga
    18781 acggtacttt cttgaaaatc catcacttgt tcaggacttc ccaactcatt taaagctaaa
    18841 gcattgtaaa aaaatggacg tctaactata tattcgcttg tatatagtat atcttgattc
    18901 gtggaatcat agggtttctt taccatcttt ttggtgacag ttggtagttt gagattccgg
    18961 gacttgaaaa aagctttttt cttatgctct ttcaattcca gctctttatc gaaatatttg
    19021 ctgatatagc gcccgcgatt ttcgatactg tctacatcga tcttgttgat cttaacgaaa
    19081 ccctgacccc aaatcgtagt taaccggtta aatgagataa aggggaaatc aaacaagata
    19141 atatggtagt ggattgcacc gcgttgctgc ttttcccacg tggcgagata ctttaaattt
    19201 gcttttttag ttttatagag gtcataattc agtcgtttga taaatttttt aaattcatta
    19261 tttgcatact caatgtcttg aatgttttct ttgaaagtga gagttaaaaa tttcgtttga
    19321 ttgtcaaaat tcgtgtctac aaggcgggca atctcaaatc gcatactttg ataatgtttc
    19381 tgtttgcgtt ttaaactgtc atattgtcct tgagcggata attcatcata gttttttctt
    19441 ttttcaagag taatatcatt ttcataaatc caagctaatt tttgttttcg ttccagattt
    19501 acttcgtttt cgatcgtttt acgtttggga gtaatgattg cttcataatc ataaatttca
    19561 atatacgtgg gagtttcaat gattttctga ttataagctt gcaaaaatca tcactccttg
    19621 ttgcgtattc gattgtcaaa gaaccagtca ttttctcctg atgttgatct ataaatcaag
    19681 ttaaggggga aggcctgcgt agcagccctt ctagcctatg gggtgttggg cggtcttaac
    19741 ggacagaaac ggctctgtgg gcgctgcccc gccgtttcgt cctaaccacc caacactcca
    19801 tagtatagtt agttttgtgt agtggtacct tgttttctgg gaatcaaagt tgccgtatca
    19861 gcggttgccg ataacccata actgacaaat tgtccattcg agcgtcccca aggtacaatc
    19921 accaaattgg taaatgtaat tatggctaaa ccttgttggg taagttcttc cggcgtgatc
    19981 acaggatgtt cagatttgac tttgacactg attttttcgt agcgaagttt tggtaggata
    20041 cattcgtagc tccaacctag aatttcgccc tccttttgcc agcgagtgac attgacgact
    20101 tggtattctt tttcgagtgt ttcttttggt aaactgagat cggtaatttt tattggcatc
    20161 acaattctcc ttttttatac gaaagtttat acgatttgat taaaaaatat agatggccat
    20221 ctacgacttg tattatatac gagaacttat acgtagtcaa gagattttct aaaaaaatat
    20281 atacgatttt ttatacgagt gttataatga aaaaaatgat aaggaggaat ttgaatatgt
    20341 ttggtttagt acaacctaat aaagtgaagg tgggccaacg aatcaaagaa ataaaagacg
    20401 gaatgtctct ctcttttaca gaattaggga atcgattagg tttaaaaaag ccgacgatca
    20461 gttcctacgt tcaaggctat gcgttggctc ctaagagtgt gatcaaccaa ttatctagta
    20521 tcagcggaaa acctgtcggc tggttttatt ttgggcatgt ggaagaatat atccgagagt
    20581 atttgtttct cattggtcag aaaaaaatag tggacgatta tcctgaaatc attgagaaaa
    20641 taaagcaaga attttatacg ggcgagttca aaaatcctgc ttgggagaat gagctaggct
    20701 atcctttgga ggaatttatc caagaatgtt ttgtagaata ttcgagtgaa atcatgaagg
    20761 actatgtcgt acagattacg aaagaggtac ttgaagaatc caaaacgcta aacaaactat
    20821 catcaaaagc aaaagaagaa gctgtcacga ttctttcatc tgggatcatt gactatatcg
    20881 agatgagtgg tgagatcaag tatggagaga aagatcgcat tgcgaaaatg gtgaaagaat
    20941 caataaaaac ctttgatgtg aaacaagacc tgacatttaa tgattcctat ttgattggaa
    21001 agttgatcaa tattcttggt acagatgaag atacggagct gtttctttct tctttatcaa
    21061 tgatatttac tgataagcat ttttccactc gatttggtgg ggaagaatta gtcgaaatat
    21121 ttcaatcgat gcgaccagcg ttaatcaaac tctatcggga gaaaacacca gatgaatttt
    21181 atgattggtt tgagaagtaa aataagaaaa gtggcttaga ttacaagttg gtgaaaaaat
    21241 ttttttcagc ataacatcag taatctataa aggggttgtt gattatactg tagaagttaa
    21301 gtactcaaga gattttttgt acgtaaacaa agcaaaaaag ggtgcagttt cactagaaaa
    21361 tgtactcttt tttgtgattt ttcttagtta tagagttttt ttgaaacttt aaaaatgaga
    21421 aaacatattg attttaaaga gtgtagacat agcttctttt gctaaataga ttaaacttac
    21481 tggtgagtgg tcttgaacat acttttaaat cacattttac tgagttttat tggattttat
    21541 aattcattgg aaacctcgta cttaatctgt ttttatctat cgactagaat ctgtatatat
    21601 tccagctatc aatgaggccg cagaaaaggg tgcttaaaaa taatctgttg tttaaaatat
    21661 tattatcaga atacttagat acaaaattct ggcacaaagt aaaaaaaata ttgacttaaa
    21721 aaaatggata tggtaatcta taaacgaaaa tagtggtaac gtttccaaaa tgcatcttcg
    21781 ttaaattttt atttttttaa acgaaaatgg aaacgtttct aaaatgtatt gggaggagat
    21841 tgaattggaa aagacattgg gaccaggtgg tgagataagg gcggtacaac gggataaaaa
    21901 aaatatttcg aagtgggaaa aattaaagaa acagttgcgg cgtgataagt ggatgtactt
    21961 gttgctctta ccaggtatca tcatgacgtt tatctttaag tatgtgccaa tgtacggtgc
    22021 tgtgattgca tttaaagact ataatcctat gctaggaatt ttggaaagtc cttgggtagg
    22081 actagatcac tttagggaat ttatcagttc accaaatttt acgcttttgc tgagcaacac
    22141 attaaaaatt agtatttatg gtttattgtt aggttttttt ccaccaatta ttttagcact
    22201 gagcttgaat caaattatca ataaaaaaat aaaacagaaa atccagttga ttctatatgc
    22261 gccgaatttt atctcagtag ttattattgt ggggatgctg tttttgttcc tatccgcaaa
    22321 tggacctatt aatgggttag tagaaaaggt tacggggcaa ccaattatgt tcatgtctaa
    22381 tccagcttat ttcagaacga tttatatttt atcgggaatt tggcaaggaa tcggctggtc
    22441 atccattctt tacaccgcaa cgctggcggg agtcagtacc gatttatatg aggcagcaaa
    22501 tatcgatggg gcaaatattt tacaaaagat ttggcatatc gatttgcctg cattgaggcc
    22561 tgtgatggtg atcagcttca tcttggccgc cggcggtatt atgaatgtag gatatgaaaa
    22621 agcctacttg atgcaaactt caatgaacat ccctgcctca gaaatcattt ccacttatgt
    22681 atacaaagtc ggcttgcaat caggtgatta tgcctactct gctgctgttg gtctgttcaa
    22741 tatgattatt aatgtcgttt tattactgat tgtcaacaat ataaccaagc ggctaaacga
    22801 tggtcaaggc ttgtaatgaa aggagaaaat tatgaatagt gctttatcaa aaatcgaccg
    22861 tcaaatctta tggttgaatc gagtgattgt aactttttta gtcgtcatca ccattgttcc
    22921 gctgatttat gtactggtag cttcttttat ggacccagct actttgatca ataaaggtat
    22981 aagcttcaat ccgaaagatt ggacaataca gggatatcaa cgggtatttg cagatgattc
    23041 cattttacgc ggttttgtaa attcgttgtt ttactctttt agcttcagct tattgactgt
    23101 atttattacg atgatcactg cgtatccttt gtctcgacct aacttggcgg ggaaaaagat
    23161 tattatgact ttttttgtca ttacaatgtt cgtgggtggt ggcttgatac ctacctattt
    23221 attggtaaag aacttgggca tgttagatag tgtatgggcg attatcgtac caggtgctat
    23281 aaatgtgtgg aatattatct tagcccgtac ctattttagt gctttgccaa aagaactttc
    23341 tgaagctgct actatcgatg gcgccaatga cttgcaaata ctgttcaaag tcatgcttcc
    23401 attagcgaaa ccaattatgt tcgtattgtt tttatatgcc tttgttgggc aatggaactc
    23461 atactttgat gccatgattt acatcaaaga tcccgactta cagccgttgc aactcgtatt
    23521 aagaaatatt ttgatccaaa accaaccttc acaagacatg gtgggagcca atactgccat
    23581 ggcagaaatg aaacaaattg ctgagttgat caaatacgca acgatcgtca tttctagtct
    23641 gccattaatc ataatgtatc ctttcttcca aaaatatttt gataaaggtg ttttagttgg
    23701 ttctgttaaa gggtaagtat tgttcactat ttgtatgaat aaaaattata aaggagattt
    23761 taagatgaaa gcaaaatgga tgctgccaat cgtttttact tccgtactaa ttttgggggc
    23821 ttgtggaggc tcaaaagaaa agagtaaatc aagccctgat tatgaactag aaaatgtctc
    23881 ttttccattg aaagaaaaag tatctttaaa aatgatgtct caaagtgccc ctttggctcc
    23941 caatgatcct aatgacaaat tgattttcca acgattagaa gaagagacca atctccatat
    24001 tgattggaca aactacagta gtgattttgc cgaaaaaaga aatttggata tcgctagtgg
    24061 tgatttacca gatgccatct tcaacgccgc tgcaggtgac tatgatttat taaactgggc
    24121 ggaaagtggc gttattgtac cgattgaaga tttgatcgaa gattatatgc caaatctgaa
    24181 gaaaattttt gatgaaaacc ccgaataccg acaaatgtct actgcacctg atggtcacat
    24241 ttactctatg ccttggattg aagaattagg cgaaggaaaa gagtcgattc atacagtaaa
    24301 tgggatggct tggatcaaca aggagtggtt ggataatttg ggtcttgaaa tgccgcaaac
    24361 cactgatcag ttgatgactg tgttggaggc tttcaagacg caagatccaa atggtaatgg
    24421 tgaagcagat gaaattcctt ttagctttat taacaatgga ggaaacgaag acttaaaatt
    24481 tttaatgggg gcattcggtc taggtgataa tgatgaccac ttagtcgttg ataacgatgg
    24541 tcaggtacaa tttactgcta acaaagaagg gtataaagaa gccatcaaat atttcaatga
    24601 aatgaataat aaaggtctta ttgataaaga ggctttcgaa caagattgga atacgtatgt
    24661 tgccaaaggg aaagaacata agtatggcgt gtactttact tgggataaag caaatatcac
    24721 gggtgcagat gatagttatg acgtcttgcc agtcttagcg ggaccagatg gaacgaaaca
    24781 tgttactcgt acgaatggca tgggtttctc aagagatcgt ttcgtaatta ctagtgcgaa
    24841 taaaaacttg gaacttacag ctaggtggat agataaaatg tatgaaccgt tacagtctgt
    24901 ccaaaataac tggggaacct acggtgatga agcacaggcg aatatttttg aattaaagga
    24961 tggtatgtta aaacatttgc ctttagaggg gacagcaccg ggggaactaa gacaaaaaac
    25021 cgaagctgga ggaccattag cagttcttga ttcttattac ggcacagtca ctactatgcc
    25081 agatgatgcg aagtggcgtt tagatttgat gcatgacacg tatttgccat atattacaga
    25141 agacaatatc tatccacgtg tctttttaga aaaagaagaa acagatcgat tagcaaaaat
    25201 cgaaacggat atgtgggatt acatctatcg caaaagagca gagtggatca ccggcggtaa
    25261 aatcgatgaa gaatgggaag aataccttgc tgaattggaa cgtgtaggtt tggaagaatg
    25321 gattcaaata aaacaaactg gctatgataa ttttttgaag ggagcacaat aagtgttagt
    25381 agaagaaacg gcgattttgg ataaagcaaa tcgatttatt gaaatgatgc gaggaaaagt
    25441 taaccctcag tatcgacaaa actatcattt ttccgcaccc atcgggtgga taaatgaccc
    25501 caatgggttt atatattaca aagggcatta ccatctcttc tatcaatact atccttacga
    25561 tagtcgatgg gggcccatgc attgggggca tgcgcgcagt agtgatttgg tccattggga
    25621 agatctacca gtagcgttag ccccagatca accttatgac agcggcgggt gtttctcagg
    25681 aagtgcaatt gaaagagacg gaaaactata tttattttat acaggctgtc aagaaattga
    25741 tggtattagc catcagcatc aatgtttggc ggtctctgaa gatggtattc attttagcaa
    25801 atacagtggt aatcctgtgg tagcagaaaa acagctaggt gaaaatgggc taatacaaga
    25861 atttcgtgat cctaaagtaa ttgttcacaa cgatggttac tatatggtag tagctactaa
    25921 atctacacag tctactggta gaatagtgat gtttcaatcg tcagatttgt atacttggca
    25981 cttttactct gtgctgctag aagggacttc agagcaagga ataatgtggg aatgtcctga
    26041 tttattccat ttagacggac gagatgtctt aatcatgtct ccgatacaaa tcaagaagaa
    26101 cggactggaa tatcataata tcagttctac catggcttgt attggtgagg taaactggga
    26161 aacaggtcag atgatagttg ataatttcca tgaaatggat tttggaatgg atttttatgc
    26221 accacaaaca acaagagatc ctaatggtgg acgggtgatg attgcttgga tgcagatgtg
    26281 ggatcgtaca ttgcctacac atgagttagg ccatctttgg gcaggctcta tgagtttgcc
    26341 ccgagagtta agagtgaaaa aaaatcgatt ggtacaaaaa cccatctcaa ctgtatatag
    26401 tcatttagtt tatcaacaag gcagtgaaaa tattgcagtg actgacacat cagtcgtatt
    26461 tcgaaacgcg ctgtctgatt cttcatattt acatctagtc gcagatttgg agaaagctga
    26521 agtttttaca attgaattac tgcggtctaa gacggattgt ctcaaactag cctatgacac
    26581 tgctacacag gtttttgaat tggatcgtca gaaactagga agagagctta caggggcgga
    26641 agaagaccct ctgaatgtac gaaaagttga agtacctttg attgataatc agctcgttct
    26701 tgagattttt agagatacct catcaattga agtctttatt aatgggttag aagttatgac
    26761 ggctactttc tatgagctgg aaaaagctcg agatatcttg tttcattcga tcggagaaag
    26821 tcgtattcaa gcttttgaaa cagcgagtgt gaaataaatg tttgattgct taggcgatga
    26881 atcaattatt taataggttt tgaaggataa tgtttttaga aaagaacaga tagctagatt
    26941 tcccatggat ttgctattag tattttgaaa aaattagtaa tttatgacaa aattcattat
    27001 actaaacttg tgcagaaatg tgagggatca actatgaaag caacaatgaa agatgtcgca
    27061 aaattagcag gtgttggagt aggaactgtt tcccgggtat tgaataacgg ttctgttaaa
    27121 gcttctacta gagaaaaagt tgaagcagca atgacagagt taaactatca accagatttt
    27181 tatgcccggg ggttaaagat gaagcatacg aacacggttg ctttgattct acccacggta
    27241 tggcatccat tttttggcga atttgcctac catgtagaaa cagcattaga aagacaaaac
    27301 tacaagcttt atttgtgtaa ctccagtggt aatagtgaaa aagaacaaga atatatccag
    27361 atggtcagcc aaaataaagt agacggcatc attggaatta cttattcaga tatcgatgaa
    27421 tacatttcga gtaatctgcc ttttgttagt attgaccgtt atttcactga agaggtgatt
    27481 tatgtgaccg ctgacaatgc ggccggtgga cgtttggctg caaatgaact ttatcgccga
    27541 ggatgtcaga atgtagcata tatcggtggg taccaagaaa caccgaatga aacgaagagt
    27601 cgtcgaaaat attttgaaat cgagtgccgt caactgggga tttcctataa agcgttagat
    27661 atgttagaac ctttagacca tctctcagaa cgtgtaacaa attttttgtt agagaatccc
    27721 gatatcgatg ggatttttac gatcaacgat ccaatggcat tagctgtcat tgatatgctt
    27781 gaaaaaatag ggaaaaacgt accagaggat gtccaagtta tcggatttga tggtcaaaag
    27841 atgacagaag gagatcctta tctagtctca acgattgccc aacctacaaa gcaaatggcg
    27901 gaagtggcag tggcaaaatt aattgctgtc attaatggag agacagtaga aaaacgtacc
    27961 gtattgccga ttgaatttgg cgaaggtgac acaacgaaac ctttagtgag aaacgatttt
    28021 gcttagtgca aatacgtttc tttttttgaa aatgagtgaa tgaaaggtgt agataaaatg
    28081 ttatatggtg gaatagaagc cggtggaaca aagtttgtct gtgggattgg taatgaacaa
    28141 ttacaagtga tagaacggaa aagttttcct accacaacac cagaagaaac gatggagcaa
    28201 gtgtttgatt tctttgataa gtacaaagga aatatcgcat caattggtgt aggagcattt
    28261 gggcctattg atattcaaat cgaatcaaga acttacggat atatcaccaa cacaccgaag
    28321 ttagcttggc aaaattatca ttttgtagga gctttggaaa acgccttgtc ggttcccatc
    28381 tattggacta ccgatgtaaa tgctgcttgt tatggagaat atatggcagg gtatggaaaa
    28441 ggtaaaaata gtgttctgta ttacaccgtt ggtacaggtg ttggtggtgg tgctgtagtg
    28501 gatgggaaaa tagtcgcagg ttttagtcat cctgaaatgg gacatatgct cttacaacgt
    28561 cataatgaag atcaatttga aggaatctgt ccttttcatc atgattgttt ggaagggtta
    28621 gcatcaggac ctgcgattga aaaacgcttt ggaagaaaag cccagactat ctcatttgat
    28681 catccttatt ggacgattga ggcagattat cttgctcagt gtgtgtataa cacaacgtta
    28741 ctatatgcac cggaagtaat aattttaggc gggggtgtaa tgaaacagcc gcaactactc
    28801 gagttgattc gccaatcgtt taaagaaaaa atgggttcct acgtggatac gcctactctc
    28861 gaagaatatc tactaactcc aaggcttgct gacaatgcgg gcttgattgg ctgcctagct
    28921 ttagcaataa agaaactcaa tcattagaat agtatttatt agaactattc ataacatacc
    28981 ggaagtttaa aagggtattc tgaaacttaa atagtctcaa ttcattcttc tttcttgaga
    29041 atctgagtag agacttttat tctgtttcaa attaatataa ttacgaaatg atattgagta
    29101 caagaaagtt ctttttacca gatcttttaa atcgaaaaat ctgatatata tatttatagt
    29161 actaaaaatc tgattagata aaaagctaat tcaataggaa aataaaggta gtgaataaaa
    29221 gcaaagccct tgttagtagt taatctaaca agggctttgg tagggaaaat ggtaggaaac
    29281 catcttattt tttacgaaaa tcaggaactt tagtaattga ataatgttga tttataggta
    29341 ttcctcatcc atattatgtt ggctctttat tcccactcca ctgttgcagg tggtttgctg
    29401 gtaatatcat acacgatacg gttaacgtgt gcgacttcat ttacgatacg gacactgatt
    29461 ttttgcaaga cttcccaagg gatacgggca aagtcagcgg tcatgccgtc aatagaagtg
    29521 acagcacgga tacctacagt gtagtcatac gtacggccat ctcccataac cccaacagag
    29581 cgaatgcctg gtaggacggt gaagtattgc cagatgtcgc gatctaaacc ggcattggcg
    29641 atttcttcgc gcaagatagc gtctgattca cggacgattt ctaatttttc ttctgtcaat
    29701 tcaccaagga cacgaatacc taagcctggt ccagggaatg gttggcgcca aacgatgctg
    29761 tcaggcatgc ctaattgggt acctaattca cgtacttcgt ctttgaacaa tgtgttcaat
    29821 ggctcgatca gcttgaattg catgtcttca ggtaatccac ctacattgtg atgtgatttg
    29881 atggtttggg cagtttcagt tcctgattcg atcacatcgg tataaagggt tccttgtgca
    29941 aggaaagaaa cgccttcttc accggcaagt ttggttgctt catcatcgaa gacatataca
    30001 aattcatttc caatgatttt acgtttttct tctggatcag acacgccagc aagttttgat
    30061 aagaaacgtt ctttggcatc gacttttata atgttcaagc caaattttcc gccaagactt
    30121 tccataactt gttctgcttc acctttacgc aacaatacgt gatcaacgaa gatacatgtt
    30181 aattgatcgc cgatcgcttt ttgtaaaaga acaccaacaa cagaggaatc tactccgcct
    30241 gaaaggccta ataaaacttt tttgtcaccg acttgttcac gaatttttgc gatctccatg
    30301 tcgatgaagt tatccattgt ccagtcacca gtacattggc agacatctag tgtaaaatgg
    30361 cgcaacaagt catttccata ttcggaatgg cgaacttctg catggaattg gattccgtac
    30421 attttgcgtt ctgcattttg gatcgatgcg atggggcaat ctttgctggt agcaacgaca
    30481 tcgaaatcag tcggtgcttg ggtaactagg tcaccatgac tcatccaaac ggtttgtttt
    30541 tcaggagtgt tttggaaaag ggccgcttct gcagaagtga tttccaattc tgcttttccg
    30601 tattcacggt ttttggctgg tttaactttt ccgcctaatt tataagtaat caactgcata
    30661 ccgtaacaga ttcctaaaac tgggatcccc aaatcaaaaa tcttttcatc aataccaaag
    30721 gccttctcat cataaacact gtttggtccg ccagaaagga tgattccttt agggttcatt
    30781 gcctttactt cttctgcggt aatgcggtgg ctcatcaact ctgagaaaac accgaactca
    30841 cgaatacggc gagtgatcag ttggttatac tgactaccgt aatcaagtac gataatttt
//
LOCUS       DAZAID010000023        29479 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000023 DAZAID010000000
VERSION     DAZAID010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.023"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(285..1676)
                     /locus_tag="ACG9BE_001010"
     CDS             complement(285..1676)
                     /locus_tag="ACG9BE_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFN3031774.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1889..2275)
                     /locus_tag="ACG9BE_001011"
     CDS             complement(1889..2275)
                     /locus_tag="ACG9BE_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFN3031775.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2331..2954)
                     /locus_tag="ACG9BE_001012"
     CDS             complement(2331..2954)
                     /locus_tag="ACG9BE_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFN3031776.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3018..3686)
                     /locus_tag="ACG9BE_001013"
     CDS             complement(3018..3686)
                     /locus_tag="ACG9BE_001013"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFN3031777.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3698..4138)
                     /locus_tag="ACG9BE_001014"
     CDS             complement(3698..4138)
                     /locus_tag="ACG9BE_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031778.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4197..4868)
                     /locus_tag="ACG9BE_001015"
     CDS             complement(4197..4868)
                     /locus_tag="ACG9BE_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031779.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(5053..5451)
                     /locus_tag="ACG9BE_001016"
     CDS             complement(5053..5451)
                     /locus_tag="ACG9BE_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFN3031780.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5448..6221)
                     /locus_tag="ACG9BE_001017"
     CDS             complement(5448..6221)
                     /locus_tag="ACG9BE_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFN3031781.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6218..6901)
                     /locus_tag="ACG9BE_001018"
     CDS             complement(6218..6901)
                     /locus_tag="ACG9BE_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFN3031782.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(7068..7955)
                     /locus_tag="ACG9BE_001019"
     CDS             complement(7068..7955)
                     /locus_tag="ACG9BE_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3031783.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7952..8821)
                     /locus_tag="ACG9BE_001020"
     CDS             complement(7952..8821)
                     /locus_tag="ACG9BE_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3031784.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8958..10244)
                     /locus_tag="ACG9BE_001021"
     CDS             complement(8958..10244)
                     /locus_tag="ACG9BE_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3031785.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10517..11095
                     /locus_tag="ACG9BE_001022"
     CDS             10517..11095
                     /locus_tag="ACG9BE_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN3031786.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11095..12024
                     /locus_tag="ACG9BE_001023"
     CDS             11095..12024
                     /locus_tag="ACG9BE_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3031787.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            12021..13757
                     /locus_tag="ACG9BE_001024"
     CDS             12021..13757
                     /locus_tag="ACG9BE_001024"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3031788.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13820..14656)
                     /gene="pheA"
                     /locus_tag="ACG9BE_001025"
     CDS             complement(13820..14656)
                     /gene="pheA"
                     /locus_tag="ACG9BE_001025"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFN3031789.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14653..15162)
                     /locus_tag="ACG9BE_001026"
     CDS             complement(14653..15162)
                     /locus_tag="ACG9BE_001026"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFN3031790.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15155..16450)
                     /gene="aroA"
                     /locus_tag="ACG9BE_001027"
     CDS             complement(15155..16450)
                     /gene="aroA"
                     /locus_tag="ACG9BE_001027"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFN3031791.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16463..17554)
                     /locus_tag="ACG9BE_001028"
     CDS             complement(16463..17554)
                     /locus_tag="ACG9BE_001028"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFN3031792.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17571..18737)
                     /gene="aroC"
                     /locus_tag="ACG9BE_001029"
     CDS             complement(17571..18737)
                     /gene="aroC"
                     /locus_tag="ACG9BE_001029"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFN3031793.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18739..19803)
                     /gene="aroB"
                     /locus_tag="ACG9BE_001030"
     CDS             complement(18739..19803)
                     /gene="aroB"
                     /locus_tag="ACG9BE_001030"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFN3031794.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19836..20858)
                     /gene="aroF"
                     /locus_tag="ACG9BE_001031"
     CDS             complement(19836..20858)
                     /gene="aroF"
                     /locus_tag="ACG9BE_001031"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFN3031795.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21517..22491)
                     /locus_tag="ACG9BE_001032"
     CDS             complement(21517..22491)
                     /locus_tag="ACG9BE_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFN3031796.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22472..24355)
                     /locus_tag="ACG9BE_001033"
     CDS             complement(22472..24355)
                     /locus_tag="ACG9BE_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFN3031797.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24373..26598)
                     /locus_tag="ACG9BE_001034"
     CDS             complement(24373..26598)
                     /locus_tag="ACG9BE_001034"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN3031798.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26713..27747
                     /locus_tag="ACG9BE_001035"
     CDS             26713..27747
                     /locus_tag="ACG9BE_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3031799.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27814..28827)
                     /locus_tag="ACG9BE_001036"
     CDS             complement(27814..28827)
                     /locus_tag="ACG9BE_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3031800.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28905..29093)
                     /locus_tag="ACG9BE_001037"
     CDS             complement(28905..29093)
                     /locus_tag="ACG9BE_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFN3031801.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         8482 a         6382 c         5209 g         9406 t
ORIGIN      
        1 actcagagtg aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa aagcttgcat
       61 ctgcggtaat aagctcaacc aaacgtaaaa tatgaggaac tatttttgtt tggctgttct
      121 tattacttga tgcagggcag ctttttcaca acctcttatt cacggtgttt cagaagtagc
      181 ttctcggaaa taagccgaaa tttcacaaaa atttgagaaa ctatttttag gaaacttctt
      241 cttatttctt aaagctgacc acttctatca caacctcttt ttgactatgc attcacatcg
      301 atcactttag aatcatcatt gatgacatcg cgatctaaat ctttgatgcg tgcacttgtc
      361 acgataccag ctaccatgct accgctgacg tttactgctg tacgtgccat atcgattaat
      421 ggttcaacag agataactaa cccaacgatt gctactggca agttcattgc ccctaaaacg
      481 atcagtgaag caaatgttgc gcccccaccg actccagcta caccaaaaga actgattgtt
      541 acaattgcca caagcatcaa aataaattcc aaactaaaga cattcactcc agctgctggt
      601 gcgacgatcg ttgccaacat agctggataa acgccagcac agccgttttg tccaatagac
      661 aaaccaaaac ttgctgagaa attagctgtt gcatcatcta ctcctaaagc ttttgtctga
      721 gtctcgatat tcaatggcaa agctccagca cttgaacgtg aagtaaacgc aaagctcaat
      781 accggaaagg cttttttgaa atagttgatt ggattgactt ttgcaccaat caaaatcagt
      841 gagtggacaa tcaaaacagc aagcaatgct acgtaagaag caacgacaaa tttacctaaa
      901 ttgacgatag ctgcaaaatc acttgttgcc aaaacgtttg tcatcaaagc aaatactcca
      961 taaggtgtca aacgcaaaac aagcgtcacg atccgcataa tgattttata cagactgtcg
     1021 actaaatctg aaaagaatgc tgcttctttt ggcgcttttc ttttcacgcc tagataagcg
     1081 attccgacaa atgccgcgaa aataacgact ccaatcgtac ttgttgcacg actacctgct
     1141 aaatcagcaa aaacgttttt agggataaag gataaaatct gctgtggaat cgataagttt
     1201 tcaactgttt cctgacgtgt tgccagttca gagattcgtg ctgtttctgc agctccttgt
     1261 gtgaatttag ctccttggag tccgaacagc attgttgaac caattccgat caatgctgca
     1321 atagctgttg ttccaagtaa agaagtcaat acagtcgcac taattttccc tagcttttca
     1381 gaaactttca tttttgtgaa tgcaccaatg atcgaaataa acactaaagg cataactagc
     1441 atttgcaaga atgatacata tccttgacct acaatcccga tccattccat tgattgagta
     1501 acgactttgt cgtctacacc aaagatcaat tgaagcagac cgccaaagac gataccaact
     1561 cctaatgctg taaagacgcg tgttgaaaat tttgtatggc gcttttgcat acgatagaac
     1621 acatataata atgccgcaaa aactagaacc actaaaactg tgatgagtgt tgtcattttt
     1681 ctcctcctca tattattaat gacaagtcca tcgaaggaag taaaggttcc cgagaatgat
     1741 tcttcaacca tcctcctgaa aaaatttttt tcagtgactt ttatatttta gacgattttt
     1801 atcgaaatga cattcttttt gcttacaatt agtaataaaa aaacatacct gtgatagctt
     1861 taatctatcg caggtatgtt tttactgtct attcttgttt ttctgattga aaattcaatt
     1921 ttaccccagg ctgatctaag gcgatctctg tcacctcata agacattcgg cgtacgatat
     1981 cgaataacgg tgctactagc tcgtccactt cttcttgtgt gacatcattt tgaccttcaa
     2041 ttttccgatt gaggatatga ttgatctgac tcacacttcc agataaaaca tatgtatcaa
     2101 acactaaacg gaactctaag cgtgctccta cgatgctatg ttctttcgga tagttttcga
     2161 tcgtttcttt taaaggcgag aagcctactt tcaaatctgt ttctacttct tggttcggca
     2221 atctcatatc ataatgaaat gcttctacaa cttctttttg tcttctgatt tccatataat
     2281 tttatcactc ctttatgaaa attataacat atctcctttg aaaggggata ctacttttta
     2341 atgatgctct gcaaggtaag gatttcattc agatttccta ccacataatc tgccgggatc
     2401 tcacccagaa aatgatcgat gtcgtaaaga caagtagcga ctcctgcatt tttccctgct
     2461 tcgatatcta aacgacgatc gccgatcatc atcgtgtctg ttctttctaa atgaaaagta
     2521 tcgatcaaat aatttaaaga tgctggatca ggtttacgag gaaagtcctg atcgatccct
     2581 actacttcct caatcagatg tgccaatcga tgttctttta acaggcccca tgttgattct
     2641 gtcaaacggt gggtcaggat gaaatgacga ccgccgttgt cttttagcat ttccagtact
     2701 tgtttcgttt ctggaaatgg acgggaaact tccgtacttt ctttttcata tcggtgaaat
     2761 aattcattaa actctctggc attcaagttt gattcattga tcaaataact agtagaatac
     2821 tttttcattt tgaagtagat ttcttttttg tccatactga taccaaaatc ttttagtgct
     2881 tgccatgcac catctaccat tgcgggatac gtatcaaaca atgtaccatc aaagtcccat
     2941 atataatttt tcatgttcct tttgttccat tcctttgctt cttttttcta tctctcactt
     3001 ttagataaag gtgttcttta aagtattttt ttgtccagaa ttgtcaaatc atctgctaat
     3061 gtatactgga ttggttgtgc atttgggata tcgatcgtat ccatttgatc ttcaggaaca
     3121 tcttctaaat attttgtcag tgctcggatg ctattaccat gacccacaac tagtaaatct
     3181 tgccgatctt ttagttttgg tgctagatga tcttgccata agggaatgat ccgattcact
     3241 gtcatttcca gactttctcc gtatgggatc aaatgcggat ccaagtgatc atatctgcgg
     3301 tcaaatctat tttctgcggt taacggcgga acttccgtat atcctctgcg ccatgttttt
     3361 acttgatctt ttccgtatat ttctttcatc ttatctttgt ttttacccac aagatcccca
     3421 taatgccgtt catttagacg ccatgttttt attataggaa gccataactg acccatttct
     3481 tctagtgcga cttgtccggt aagaatcgca cgtttcaaaa cagaggtata aacggcatcg
     3541 aattgaagtc cggcttcaag cattttttga ccggcaactg ctgcttgttc ttttcctttt
     3601 tctgttaagg cgacatctag ccatcctgtc cagtaatttt caaaatttgc ttcgctttcg
     3661 ccatgacgta ataatactaa tttcattctt ttctccctta aacattcgtt tcgaatttta
     3721 taccacatgt tttgcatgtc acacgtattc gtccttttcc tttaggtgca cgctgctttt
     3781 gatgacaatt cgggcactca aaaaaagtat aactttttcg atccattttg acttgctttt
     3841 tgttttttat ctgatgatat ttttctttga ctctatcaag atatttcaaa taacgttgat
     3901 tctcatttag tctggggtag attttttttg ataggaaacg aaatatcatc catccgaaaa
     3961 agacgatgaa caacaattgt ccgattctat agggcaacca acgacttagc aaactaaaga
     4021 caagcgcagc gatcaataga ccgcgattca actgatcaaa tttcgcatat cttccctgca
     4081 taagtttgtg atagttctgc tgaaatcgta ttagcctttc taaccatgtt tgcatcatat
     4141 tatctcctcc atcatctaat atttattatg acaaaaaaat ccaaaaagaa aaagccttaa
     4201 ttggcctttt ccattgcaac ttggtgaagg acattccgat ttctggtaaa gtcaatcaca
     4261 atcactttct ttttatcttc tctgttgact actcgaaatg tgtccgtcaa tggaagaaac
     4321 agcttcttag atggtagctg ttgcatacgt ataaaacgat ggatcatcgt aatcaattga
     4381 ggaaattgaa tagacgtgtg aagcagacct ggatattttt tcaacagctg tgtcgttttg
     4441 atcagattcc gttttttact gatcactttt aaaaagcttt ttagaatctt ttgctgacgt
     4501 ctaaaaatag aaaaagaatt ttcgtcatct atttgataac gcatttgttt ttgcagttct
     4561 gctgcagtca gatggatctt cccatcgttt gtatcgatca ttaaaccttc tggaaacagt
     4621 tcaaccactg ctcgatccca agattcaaga gaaagctcga tataatcttt tactggtaat
     4681 tccaattcta ccttaaaaaa gtgcaaaaca gccgacagcc cttctgcttg atagatttct
     4741 cctaatgtta gcccctctag atggaactct ggtgaaaaat gcagcaatgt tttattttcc
     4801 tgcaattgaa catatagaaa tgtaaatcgc tcaatcagag tatcttttaa aacaactaaa
     4861 aaattcattt ttctccccct tttttcatca tttttaaaat agcatatttt gaaaagaaag
     4921 tctgatgctt agagtagaat ttcataaaaa tttactattt cataagtatt cacgagaaaa
     4981 atacaaacaa aaaagcggaa ataacgctca tccgccttct tgtttgtgtt tatttcctct
     5041 tcaaacgctt gtctatgatt cagacttttc tctatcgtta ttttcatcta aaagttcttg
     5101 cattccgata atttctcgat attgatgaat cttagtgata ccaatgactt tgtctacatc
     5161 aatactaaaa caaataccat tacctggaat atcgatgttg atgccttgac caatcgcatc
     5221 cattacttta ttcgtcaaac tatcgggtac taatgtcaac acaatatctt tttctggcga
     5281 aatgtccaaa cctaaaaatt tccctgaatc atgtacacca gaacctcttc catgtaagat
     5341 cgttccgcct gctgcacctg cttcttttga aaagtcgata acatcttcgc ccattcctcc
     5401 gtcaacgatc gtcacaatca tgtgaagatt caatgctaca agatctttca tttgctttcc
     5461 cccaattttt gcaatctttc tttttcgatt ctgtcttttt catcttttcg tttataaata
     5521 tatcctagta ctaaagtgga taatattggt gtcaatgcaa tcattgcaac cacaccaaac
     5581 ccgtcaatga tcggatctct tccttcaatt tgtgcagcga ttgccacgct cattgataaa
     5641 ataaatgtcg aacacatcgg gcccgttgct actccaccgt tatcaaaagc cattgctaaa
     5701 aagatcttgt ccacttttcg tcctaaaata aatgctagcc cgtatcctgg gatcaagaaa
     5761 taataaagag agaatccgac gagcaaacgc cacatagaca atcctactgc tgcgccgatc
     5821 ccaattgaaa ttaccatcag catgattttc gatcggattc tcccctcgct caattcttca
     5881 acctgtttga tcattacatg aatcgccggc tcagcaaacc ctactaagaa ccccattaaa
     5941 aagccaaggg gaaccagtat ataattgtaa ggcaatcccg caatagcact gcctaaatgc
     6001 tgtcctaccg gtacataagc catattgact ccgtgcagga aaaggatcaa cccaatagtc
     6061 gttatcaaga aacctttcat aatgtcaaag aaacgctttt tgcgcatacg aaaactaatg
     6121 atattcatta aaacaaatag gattacgata ggtgccactg ccaaaatgac ttccatggta
     6181 acgatttcga ttccatctat cacttgctgc cagaaactca ttggaagatc acccccaaaa
     6241 tcatcacggc aagaatcggc cctaacgaag caataccgac cattccaaaa ctgtcatttt
     6301 catttttctt ttgtttgatc atactagtca tccctccggc aagggccaag ataaatggaa
     6361 cagttactgg accagtagtc actccccccg aatcaaaagc aatcggcata aattcggaac
     6421 tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat agccatcaat tgataatagg
     6481 atagcttgaa tacgacccga agcagcgcaa aggctagaaa tactccagtg ccgacgctga
     6541 cgatcccaat cagcaacact ctgccaatct ctccgtcaga tatatcatat acttgttgtc
     6601 ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat cccgatagca aatcccaaac
     6661 tgaccaaaac ggtcaaactt ttctttttga tcatatattt ccctactaga tctccgactt
     6721 ccatcataga ataatctgct ccaaacaaaa agagcgtcat tccgatcatc atgattgccg
     6781 caccaaccag aaatgaagcg aggtcttctc cttctaacgg tgcaaaaaca aacgttaaaa
     6841 tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa ttttttagca
     6901 tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt cgagcacaaa
     6961 cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc aaaagtatct
     7021 ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta ttttacagag
     7081 ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa
     7141 gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga agcacccata
     7201 gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac taagggccat
     7261 aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata ggtattttta
     7321 gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc taatcgagcg
     7381 gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa gatcaaaaaa
     7441 gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa aaccgttccg
     7501 ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg aaccatcatc
     7561 gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat ctcaaatcca
     7621 tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc gatcatcgct
     7681 gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg aaaattgatg
     7741 atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt attcgtcatt
     7801 cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct taatgctgta
     7861 tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt gacgttgggg
     7921 cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct actttcgttt
     7981 ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct gccgcatatc
     8041 caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg gtggtcgttg
     8101 catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc aatgtcccga
     8161 tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc gtcactttga
     8221 taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa
     8281 tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc caagtgatcg
     8341 aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa
     8401 tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat agacttttca
     8461 tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg gttcgaaaca
     8521 accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt gctagaaaca
     8581 acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct tttttaaaag
     8641 ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata ttgacagggg
     8701 gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc atgattgata
     8761 tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct ttctgcttca
     8821 ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa agacaatatc
     8881 tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt
     8941 cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg tgtatctgct
     9001 agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc tacgactgct
     9061 tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt ccatgttgaa
     9121 aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata agcttcttgt
     9181 tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt gcccatcagt
     9241 tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc tccagctact
     9301 cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt ttgcagattt
     9361 tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc tgctccttgg
     9421 atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga ttgtatcgca
     9481 gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa aagagtggca
     9541 aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt ctccccatct
     9601 tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa
     9661 ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc tacttgaata
     9721 tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc tgccatcaga
     9781 tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta tattttatct
     9841 cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac aatagcagac
     9901 aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata atcttcaatc
     9961 aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt cagtttctga
    10021 acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt ctcataaact
    10081 ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca gacagtgact
    10141 ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa acctgatgct
    10201 gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat ccgctccttt
    10261 tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga aaacggtttc
    10321 tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac aaatcaacta
    10381 tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg tttctttaaa
    10441 ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca agactattct
    10501 taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac agacgatttt
    10561 agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa tagggacttt
    10621 ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata tcgtggtcac
    10681 agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa aacagcaaca
    10741 acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg ttaaagattc
    10801 ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg atttttttct
    10861 aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac
    10921 caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac aaactcgtcc
    10981 attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac cgaaaaacgg
    11041 ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc attgatggat
    11101 agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc cggctatgta
    11161 atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg tcaagaagtt
    11221 tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat caatgaaata
    11281 ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg aaagaacctt
    11341 gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc aagtagttat
    11401 ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga gaatttcttc
    11461 acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc aatcaatcgt
    11521 ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga actttctttg
    11581 ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga tttttctatt
    11641 cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa aaaagacaat
    11701 ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa agaaatcaat
    11761 ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc catctttact
    11821 gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag taaagccaag
    11881 ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt cggctatacc
    11941 gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc ttctcaatac
    12001 cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc tttgccaaac
    12061 ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct ttgtttattt
    12121 atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat gtggtactaa
    12181 aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca ttagccgctt
    12241 ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa catatggaac
    12301 agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa atccatccaa
    12361 tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc ggaaacgaca
    12421 tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag agagtgaatg
    12481 aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt cgcgataaga
    12541 aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac gtttacggat
    12601 atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct gtagtaaatc
    12661 ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca tcaaatgaag
    12721 aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt gaaacaacga
    12781 tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt acattactca
    12841 gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg attattttgt
    12901 ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg gatcgacaca
    12961 caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat ggcttaaaag
    13021 ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg atcaatcaat
    13081 tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag aagaaacgtt
    13141 cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat aatacgctga
    13201 ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa ggaatcgaca
    13261 gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt ccagtcaagg
    13321 aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat ggcgaaagaa
    13381 tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg aatatgatcc
    13441 ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg caaggattca
    13501 tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt gataacggcg
    13561 atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga atcggcatca
    13621 gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta acgatccaat
    13681 cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct aaaaatcaat
    13741 cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag ttaaataaat
    13801 caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact ggataacagc
    13861 ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc tctactaatg
    13921 tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag ctagttttca
    13981 atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct gccagcattt
    14041 tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt tttactggag
    14101 ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc tggttcaact
    14161 cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct gtttctaatg
    14221 aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct gcagcagttg
    14281 ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac tgttgtgatt
    14341 gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga ttgcctaaaa
    14401 gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga tgtttggaaa
    14461 gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga acgacagcca
    14521 gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca tacgcagtca
    14581 attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt tctggcccta
    14641 gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga tcttttctgg
    14701 tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag aaagtcgttg
    14761 tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc gaatagacgt
    14821 ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt aaagaacaat
    14881 cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc caccagtcgc
    14941 gataattttg tcttcatttg acagctttct taaaatatct gactccactt ttcgaaagct
    15001 tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt cctcaatctt
    15061 tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac cgattgttgt
    15121 ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct cctcctgaca
    15181 caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct tcagctcctt
    15241 ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata ccgattcgat
    15301 gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg atgatcagac
    15361 cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc accgcatcaa
    15421 ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg attccttctg
    15481 cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga atgatttctc
    15541 caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc gattggttct
    15601 gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca tctaagatac
    15661 cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc ggaacaagaa
    15721 gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt atctgttgac
    15781 cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg atccttccgc
    15841 caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca acgattacgg
    15901 atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc gcactggcta
    15961 caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg attggtagat
    16021 actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt tccatcactc
    16081 gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta aaaggctgtc
    16141 cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg tcaattgatt
    16201 cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc tcttcctctt
    16261 cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa tcttctgcgc
    16321 gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg atgctgcgat
    16381 gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt atttgttgta
    16441 ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta ttagacataa
    16501 tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc agtattccta
    16561 aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc gcttttccga
    16621 tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata aataaatcaa
    16681 aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt tcttttgccc
    16741 gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct gctatttgtg
    16801 tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg ctcaacaaaa
    16861 tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga aagcctcctg
    16921 ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga ttgactaagc
    16981 cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca tgttctcttg
    17041 ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt ttttcaacat
    17101 cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat agtttagcat
    17161 ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg acaaaccgaa
    17221 gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca tccgtaatga
    17281 tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag gttgtacgga
    17341 cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa tcacttgcaa
    17401 cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga ctatcccaac
    17461 caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa gcaccaatca
    17521 gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt ttagtaatgt
    17581 ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga atcacttggg
    17641 aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc aacgacgcta
    17701 gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg ttcttttgta
    17761 tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat gaccccgcga
    17821 atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt attggtacgt
    17881 cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga gcctggtaat
    17941 gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat actaaccaca
    18001 gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc taatccagct
    18061 ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt tttctttgtc
    18121 tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg tacttctgac
    18181 gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc tgcttggatt
    18241 ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag aattttcttg
    18301 gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg ttcaagcaca
    18361 ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg tcctggacgc
    18421 ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac agacatgaca
    18481 gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga accaattgtt
    18541 ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt catccttcct
    18601 cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc ttctgacaga
    18661 ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg ggattctccc
    18721 gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct ctgtgggaac
    18781 aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt ggcttcctct
    18841 tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta gatcgtacgt
    18901 aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc cagttggaga
    18961 caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta ctccttctcc
    19021 atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg tatgaccaaa
    19081 atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt ttcgtttgat
    19141 ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt cgtctttcag
    19201 ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga tgatttcagc
    19261 gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat caatcaatac
    19321 accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat tgactgctgt
    19381 cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt gaacgaaatg
    19441 gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca ttcctccacc
    19501 aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat cgtataactc
    19561 agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa ggactggttc
    19621 aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag caacgtttgt
    19681 atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg atccagcctg
    19741 ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat gtacagtaag
    19801 cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg aatcttttcc
    19861 attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt ttcattcaac
    19921 gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac gatcatgcca
    19981 tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca aattcctgta
    20041 ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa tgggacagca
    20101 cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg ttcgataaaa
    20161 atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca ttcattgatc
    20221 gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac agcagatagc
    20281 agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta ctcagccacc
    20341 atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa ttcatcagcc
    20401 gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata tggagaagta
    20461 cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc cattcgcgca
    20521 cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt cacgaaactg
    20581 ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg gaattctcga
    20641 gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc atagcgtgta
    20701 aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac tcgttctgtt
    20761 ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac tttttttatt
    20821 tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc tttcttttgt
    20881 ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac attaattcct
    20941 actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact tatgacccag
    21001 ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac atcttcaacg
    21061 agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt ctctacgtgg
    21121 ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt tgtttgtacc
    21181 cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat tcagttttgt
    21241 aagttttcta gatttcataa aaaatcgctc caaactgatg taagaattgt acctagtata
    21301 gacataaaaa aatcgactgt caatcatttt tatgaaactt tttttaattt tctgatagtt
    21361 aactttgttt ttcagaaaaa taacaattgg ttttattgaa gaaaatagtc atgaacaaaa
    21421 gtatctgaga acttttttac tctcttgttc atgacatata gaataaagca gctttgtctt
    21481 gtaaactccg tttttcaaat aataatctaa agattgctac tgaataataa agccgatttc
    21541 atggtcctct tcgctgctga ttcggtaagg tgactcaaca tccgctcccc agctattgat
    21601 acccccgact cctcttactt ttgcaaaaat cgtaagaacc gttcgtcttt ctggcggtag
    21661 ttcttctata tgtgttgcct gttccagttc ttctgctttg aatggtaaag cggaaaaagc
    21721 aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct tcttgatctt tatccgtatt
    21781 atccaacgtt tgatttcttg ttagttctac ccatttcgtt tccatatgca tgccgcattc
    21841 ttggggaaca aggtatgggg aaacggggag tcctgccact ttataaatac cttcgacacc
    21901 accagccatc cgatccggat atgtttcccc tgataggcct tcatactcaa aaaattttgc
    21961 tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat tccggtaatc ctttttgtcc
    22021 ataataatgc atattgacaa gcattttacc ctctccagcg atttggtagg aaattgtcac
    22081 ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg atttcaaccg tttcagcgta
    22141 ttcattactt gaatattgat tatttactgg agcaatcggc aaatcgatct ttttattgtc
    22201 caccagtact tctatttctt tgacttttgg atacaatccg gctcctaacc acataccgga
    22261 tttaaatcca aatccgctac ctctgtcatt atcggtcgtt gctcgccaaa aaactggaag
    22321 tgtttcccgg tatagccatt cttttccgtt tttattgagc gattccagtc cgcctcttgt
    22381 ataagaaaaa atataatgcg tattccccga tttgacacct aatgtacagt cgccgaagac
    22441 gattgcaact ttttccgtat ccatatgact tttatttact gtatttttca taataatatc
    22501 ttacctcctg catggcgggt ttttcttgtc catctgcaaa aacgatccca ttccctgaga
    22561 attcatagtc acttggttta tcgtcaaagt caccgccata gcgcatcact tctctgcctg
    22621 tgattggatc agtcacttgg atcgcctgat ctttaaaatc ccatatatag cctccttggt
    22681 acatttcata acgatcaagc aacgtcatgt aagaatccat tccacctaac gaattcccca
    22741 tgtcatgcat atattcacat aagatgaacg gtttttccgc tttattttca aggtagtcaa
    22801 tgatctcttc tggtggcgca tacatgcagc tttttacatc tgaaatcgaa tcttcgaatg
    22861 ctggatttgg aaaattccct tcataatgga caagtcgatc ggggtctact tttttgaagt
    22921 aatcgttcat ttgtcgaatg ttttcttccg cataagattc atttccaaga gaccaaaaaa
    22981 gaatacttgg atggtttttt aatgtttcga aattcgtttt tgctcgatca agaacagccg
    23041 cctgccactg aggtactgca cctggtacgt tgtaagaagg ctcaaatgcg cccatttttt
    23101 gccatgagcc atgggattcg aggttcgttt ccgccatcac atggattcct gcttggtcgc
    23161 ataaataata ccaagtgatc tggttggggt aatgagatgt ccgtacgccg ttgatctttg
    23221 cattttttat gatagccaaa tctttttcca tatcttctat agtgatggcg cgtccttttt
    23281 tcggatgcca ctcatgtcga tttaccccgt tgataatcat ccgttttcca ttgaagtaga
    23341 tgattttatc tttgatttcg attttacgaa aaccgaagga atagtcaata tactccgtta
    23401 ctcttttttc ttcgtctatc gtctccaatt caagcttata taaattgggc tgaaaatgcc
    23461 cccacagtgc cacattttca aatccatcga tgcgaatcgt catttgctct tttaacggat
    23521 attgctgttg atagatttcc ttcccatcag gatctagtat cgctacattc agctgactgt
    23581 ttttggctac ttgctcaaaa gacaaatcta cttcaagttt gccattttga catttgtcat
    23641 ctaagcgagg tttgatccaa agatcttcta aatggttttg tcctcgagca actaagaaga
    23701 catctctgaa gatcccaaaa aatcggaaaa agtcctgatc ttccaagaat gcagctgtac
    23761 ttcttttgta aacctccaca gctagaatat tttcttcttc caaataaggt gttaaatcaa
    23821 attcagacgg agtgaacgaa tcttcggcat acccgataaa atgaccattc aaccaaataa
    23881 acattgcttg ttctacacca gcaaaatgaa tcgagatttg ttttcccttt agaccttctt
    23941 ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc tttagcagct gaaaagatcc
    24001 cttccgcctc tttctcaggg cttgtcgtga aagggggacg gcgaaataca tgtccctccc
    24061 acggatacat cgtattgatg taatggatct tgtcataatt tgctaattca atatgactag
    24121 gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt tttataaaat gtttttggtc
    24181 gtaccgaagg atttggcgaa taacaaaaac gccatgatcc atttaaagac tgtttaaggg
    24241 aggatgtggt ttctacttct tcccttgttg cataacaaac atgatcgcta tgtgcatttg
    24301 attggttcac tctaaaaact tgtggatcat ctaaccattt tatatttgct ttcatacttt
    24361 tttccttctc tctcataact ttttgaaatg aagcagttct gtttggaaat caccttgaaa
    24421 atcggggaag taaagtccaa ctgtcatcaa ttcggccccc gaaaatatcc gcttcgaggt
    24481 ctgttcctga taaagtgcct catcatctaa ccccatcaga tatacttcat ggaacgctgg
    24541 ttgagctgaa gctagtatcc tccctaaaaa cacaatagct tcatcttgtt taggagacac
    24601 aaacatccat gcggcaatat tttcttcaaa aggactcgcc aaacgataat aatggccata
    24661 ctggatcacc ggacgaatcg tttgatagga tataatttgg tcttttactt gctttttttc
    24721 ttcaggagac agctttgtca aatccagttc ataaccaaaa acagcgctca tagctgtttc
    24781 tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga tttggtacag ctgatacatg
    24841 agctgtgatg gacgaaatcg gataagcaag gcttgttcca tattggattt tcatacgctc
    24901 cactgcatcc gtgttatcac ttgtccatga ctgcggcata taataaataa acccggcatc
    24961 aaaccgtcca cctccaccag aacagccttc ccataagatt tccggataat cagttgtcaa
    25021 atgttcaagt agttcataaa gcccgagcat ataacgatgc gctacttctc cttgtctctg
    25081 tgggtcaagg gtaatcgaat agacatctga aagcgaacgg ttcatatccc atttgatgta
    25141 gtcaagagga atcgtatcta aaatctttct catttgtttc tcaatcgtct gccgaacatc
    25201 taaccttgta aaatccagta catgttgtga tctggaagca gaaggcattc tacctggctc
    25261 ctgcataaga aaatcaggat gtgttcgata cagttcagaa tcaaccgaga tcatttcagg
    25321 ttcaaaccat aaaccaaatt tcaatccttt gctatgagca taatctgcta tttcacgtaa
    25381 tccatttgtc aacttgcctt catactcgaa ccagtctcct aatgaagaat tgtccgcatc
    25441 tctttttcca aaccagccat catctaatac gaacatttcg atgcctagct cctttgcttc
    25501 atcaatgatc gccttgattt tttcactatt gaagtcaaag taagtggctt cccagttatt
    25561 gaccagaatc ggccgttcag caaattgatg acgacctctt gcgattcttt cacgtagcaa
    25621 ctcatggtgg atctgactca ttttgttcaa tccctgactt gagtaagaaa ggatcatttc
    25681 aggtgtttga aaagactggc ctgcattcaa ttcccacgaa aaacgatggc tgttgatccc
    25741 accgacgact ctcaattgat tgatctgatc tttttcaagt tcgaaagaat ggttgccgga
    25801 ataaacaaat tgaagtccga tcgcttctcc agtattttct gtcgtatgat gatgaactaa
    25861 agcaataaag ttattcatat ggtgactgct cgttcctcga cggctttcaa atacttttgt
    25921 tccagataaa acagattgcc gtgaaatctg tctttcattg acatgtgctc ctggcaaagc
    25981 aatgacttca tcaaatcgcc tagtatgggc aaagtctagc tggaaactag ccgctttttc
    26041 tagtttgatt gtttctccgg tttcatttct tatctttaca gatcgtgtga tcactgctct
    26101 atcagtaaat atggtataga acaaatcgaa ataaatcgct gtttctctat ccttaagtga
    26161 aatcaccaac gtttgtgcct ctgacttgtc catcacataa gtttgaggca agccttcgat
    26221 ctctggcttc ccatctgtaa tataatggct gtcatatctc agatccagta agtctgatcc
    26281 attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc atgctgccat gagaagagta
    26341 ttcttgcggt agtgtatctt tcgacagaga ccggtcagga tttaagggaa catttcctga
    26401 aaatccacga tctcttcggg gataattttt attgtctttg tactgcttga ctggtttgcc
    26461 aaaataaata tgcgccaata cattgctttc ttctatcttt atcagataag aaagaaatgt
    26521 atttcgtaaa tggaagactc ggctcttttc atcaaataag atgaaatttt tatctgtttt
    26581 tctatgcgta atatccatca tttcactccc aacaaaacgg aaaccgttta ctttttttta
    26641 taattcctgt atactgttta ctaaacaatt ttgtcaataa aaatttttta tttttttgag
    26701 gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa aggcaaatgt ctctcctgct
    26761 acagtttccc gtgtgttgaa taacgatcag acgatccatg tggctcagac tactcggcaa
    26821 cggatagtag aggcagctgc tgcgttggat tatgacatca gtactcgtaa atatatcaaa
    26881 aagagaatgc cttcgattgg tgtgatctct acgatcggca aagcgagcga agaacaggat
    26941 atctattaca aacatttgcg agtagggatg gaagaagaag ctcgacgtct gcatttaggg
    27001 atgaatcgaa tctataatct atctgataat cctaaacaat gggaagatct agatcagtta
    27061 ggtgcggtga tcgttgttgg gactgttacg aaagaatcga ttaccgcctt gttaaaacaa
    27121 aacatgcaaa tagtcgtcgt ggataaccct gacatccagc aagaggtcga tatggtgtat
    27181 gcagatcttg aacggatgac taaaaccgta ctggaattgt tcttagaaaa tggtcataaa
    27241 agaatagcct atatcggggg atataaagtc gatatggatg aattcggtgg gaagcagatt
    27301 tctataaatg aaaagaggat gcgtgcctat cagcatttta tgatcgatca tcacttagaa
    27361 aaatatatgt cttatcatct aggtggctgg acagaaaaac aaggtgaggt acttgcgact
    27421 aatctattag aaaatgaatc ccctcttcct actgccattc ttgtcgggag cgatccaatg
    27481 gcaattggtg tctatcgggc attgaaacaa aaaaatatcc aaattggaga agagattgtt
    27541 gtagcaagct ttgataatat cgagacaatc gcaaacctct cccctggctt gaccagtgtt
    27601 caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa tggcattgga gcgaattgac
    27661 ggtatacggg acgaaccaat cattttgact tatcctgcac gattgattgt tagggaaagt
    27721 tttatcccaa gaacaaaaag gtattgaaag agaggttgtg aaaagccgct tcgtttcttc
    27781 atactcaaaa cgaaacggct ttttcacaac ctcttactta tacttattca caaaatccaa
    27841 tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa tcaaattggt attcatgggt
    27901 aatctctttt tttgtatctg tataaaacaa ggattgcacg ggtaccttta attctgtcaa
    27961 ccgattttct aatgcttgac cttgctcctg gaatgagtat gcatttccgt cagttatata
    28021 cgttggcgga aattccttag aaacatggtc agccacacta gcttctttca attgctcact
    28081 cgttttccaa tcttttgttc caatcaacga ccacgcaacg gttttgacga aaaacttcat
    28141 aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa tcaacaggtc cgcaataaga
    28201 aatggttcct ttcagactat cttttggaag tagctgtttg attttcattt gtttagcata
    28261 cgcatcattc gtttgtatag ctgcatattg caatgcgatt tgtgaacctg cactatctcc
    28321 accaaaaaat aattgttcca tgtctagcaa tttgtcgtct ttttcttcag ctaataaatc
    28381 tttgatcagt tcattcactt gaagaacttg attagggtat tctgaatcag gcgctaattc
    28441 ataattcatc gcaaccactg cgatgttggc gtctgaaact aattttgtcg caaattcttt
    28501 gacacccgat ttatctccgc cgacgaatcc tccaccatgt acccaaaata gaacaggaac
    28561 aggccccttg ctgttttttg gatagtagat gtcataagta ttctctttat acttggactc
    28621 atacgtcaaa tctgtttttt tcgccacgtt tttttctgct tgatcgaacg tttttttatc
    28681 agtgatttga acagtatttg aaaacatatg gttgatcaag aatgctccag gtctggggga
    28741 aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga atgattccga agaaccatcc
    28801 gataaattta agaattttct ttttcataag gctccctcag tagttagtat taagcattct
    28861 gattatacca tagtgttatt tcctatcaag ctctcctaga tgatttaatg attttcccca
    28921 aacttcttct gcaatgcttt tgacaaaaga aattttttcc attgttcttc aaacaaaaca
    28981 ttttcttctt ctgttgaagc aaatccacat tgaggactta agcacaaacg atccaaaggc
    29041 agatatcgtg tcgcttcgta gatccggtca atcacttgct ggtgctcttc catttcgcca
    29101 tttttcgtta cagtcaaaat ctaagacaac taatttatct tcagagactt tctctaaaag
    29161 aacaaaactc ccagcacgtt cactatcgta ttctaaataa taggcacgga cattttcttg
    29221 agtaaaataa tgggtctgct acgtcgtcat cccccctgat gccgccaaaa tcgaataata
    29281 atttccgcgt caaacatgta tatttagtgt tgaatcattt gagaatatga aaataaaaat
    29341 ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa atctgagaca gaatgacgtt
    29401 cattctgacc cagatttgat tttgcacaac tcaacagtcc ttattggcaa atcgccaaaa
    29461 taatgtagac taactaaaa
//
LOCUS       DAZAID010000024        29100 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000024 DAZAID010000000
VERSION     DAZAID010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.024"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACG9BE_001038"
     CDS             complement(<1..1031)
                     /locus_tag="ACG9BE_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3031802.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACG9BE_001039"
     CDS             complement(1113..1802)
                     /locus_tag="ACG9BE_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN3031803.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACG9BE_001040"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACG9BE_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFN3031804.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACG9BE_001041"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACG9BE_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFN3031805.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACG9BE_001042"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACG9BE_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFN3031806.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACG9BE_001043"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACG9BE_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFN3031807.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACG9BE_001044"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACG9BE_001044"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFN3031808.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACG9BE_001045"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACG9BE_001045"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFN3031809.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACG9BE_001046"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACG9BE_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACG9BE_001047"
     CDS             complement(7636..8523)
                     /locus_tag="ACG9BE_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN3031810.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACG9BE_001048"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACG9BE_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFN3031811.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACG9BE_001049"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACG9BE_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFN3031812.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACG9BE_001050"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACG9BE_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN3031813.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACG9BE_001051"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACG9BE_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFN3031814.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACG9BE_001052"
     CDS             complement(10454..10957)
                     /locus_tag="ACG9BE_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFN3031815.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACG9BE_001053"
     CDS             11554..12519
                     /locus_tag="ACG9BE_001053"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN3031816.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACG9BE_001054"
     CDS             complement(12618..13790)
                     /locus_tag="ACG9BE_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3031817.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACG9BE_001055"
     CDS             complement(13777..14922)
                     /locus_tag="ACG9BE_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3031818.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACG9BE_001056"
     CDS             complement(14936..15868)
                     /locus_tag="ACG9BE_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3031819.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACG9BE_001057"
     CDS             15985..17064
                     /locus_tag="ACG9BE_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN3031820.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="ACG9BE_001058"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="ACG9BE_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17789..18016)
                     /locus_tag="ACG9BE_001059"
     CDS             complement(17789..18016)
                     /locus_tag="ACG9BE_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3031821.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="ACG9BE_001060"
     CDS             complement(18119..20707)
                     /locus_tag="ACG9BE_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFN3031822.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="ACG9BE_001061"
     CDS             complement(20727..21386)
                     /locus_tag="ACG9BE_001061"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN3031823.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACG9BE_001062"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACG9BE_001062"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFN3031824.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="ACG9BE_001063"
     CDS             complement(22159..23025)
                     /locus_tag="ACG9BE_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFN3031825.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="ACG9BE_001064"
     CDS             23134..23775
                     /locus_tag="ACG9BE_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFN3031826.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACG9BE_001065"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACG9BE_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFN3031827.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="ACG9BE_001066"
     CDS             complement(25216..26115)
                     /locus_tag="ACG9BE_001066"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN3031828.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="ACG9BE_001067"
     CDS             complement(26117..26914)
                     /locus_tag="ACG9BE_001067"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFN3031829.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="ACG9BE_001068"
     CDS             complement(26892..27596)
                     /locus_tag="ACG9BE_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFN3031830.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="ACG9BE_001069"
     CDS             27727..28302
                     /locus_tag="ACG9BE_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFN3031831.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="ACG9BE_001070"
     CDS             28420..29097
                     /locus_tag="ACG9BE_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFN3031832.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8564 a         6166 c         5230 g         9140 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactac acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aatgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actatttaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
//
LOCUS       DAZAID010000025        29059 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000025 DAZAID010000000
VERSION     DAZAID010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.025"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            317..637
                     /locus_tag="ACG9BE_001071"
     CDS             317..637
                     /locus_tag="ACG9BE_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFN3031833.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            791..1696
                     /locus_tag="ACG9BE_001072"
     CDS             791..1696
                     /locus_tag="ACG9BE_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3031834.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1693..2466
                     /locus_tag="ACG9BE_001073"
     CDS             1693..2466
                     /locus_tag="ACG9BE_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN3031835.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2647..4071)
                     /locus_tag="ACG9BE_001074"
     CDS             complement(2647..4071)
                     /locus_tag="ACG9BE_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFN3031836.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4282..4728
                     /gene="nrdI"
                     /locus_tag="ACG9BE_001075"
     CDS             4282..4728
                     /gene="nrdI"
                     /locus_tag="ACG9BE_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFN3031837.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4793..6775)
                     /locus_tag="ACG9BE_001076"
     CDS             complement(4793..6775)
                     /locus_tag="ACG9BE_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFN3031838.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6789..7685)
                     /gene="murQ"
                     /locus_tag="ACG9BE_001077"
     CDS             complement(6789..7685)
                     /gene="murQ"
                     /locus_tag="ACG9BE_001077"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFN3031839.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7688..8749)
                     /locus_tag="ACG9BE_001078"
     CDS             complement(7688..8749)
                     /locus_tag="ACG9BE_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFN3031840.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8960..9487)
                     /locus_tag="ACG9BE_001079"
     CDS             complement(8960..9487)
                     /locus_tag="ACG9BE_001079"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN3031841.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9554..11056)
                     /gene="eat(A)"
                     /locus_tag="ACG9BE_001080"
     CDS             complement(9554..11056)
                     /gene="eat(A)"
                     /locus_tag="ACG9BE_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN3031842.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11314..12435)
                     /locus_tag="ACG9BE_001081"
     CDS             complement(11314..12435)
                     /locus_tag="ACG9BE_001081"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN3031843.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12561..13502
                     /locus_tag="ACG9BE_001082"
     CDS             12561..13502
                     /locus_tag="ACG9BE_001082"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN3031844.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13652..14548)
                     /locus_tag="ACG9BE_001083"
     CDS             complement(13652..14548)
                     /locus_tag="ACG9BE_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFN3031845.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14817..16157
                     /locus_tag="ACG9BE_001084"
     CDS             14817..16157
                     /locus_tag="ACG9BE_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3031846.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16375..16818
                     /locus_tag="ACG9BE_001085"
     CDS             16375..16818
                     /locus_tag="ACG9BE_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFN3031847.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17322..18740)
                     /gene="nhaC"
                     /locus_tag="ACG9BE_001086"
     CDS             complement(17322..18740)
                     /gene="nhaC"
                     /locus_tag="ACG9BE_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFN3031848.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19143..20366)
                     /locus_tag="ACG9BE_001087"
     CDS             complement(19143..20366)
                     /locus_tag="ACG9BE_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN3031849.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20397..21236)
                     /locus_tag="ACG9BE_001088"
     CDS             complement(20397..21236)
                     /locus_tag="ACG9BE_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN3031850.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21253..21933)
                     /locus_tag="ACG9BE_001089"
     CDS             complement(21253..21933)
                     /locus_tag="ACG9BE_001089"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFN3031851.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21935..23008)
                     /locus_tag="ACG9BE_001090"
     CDS             complement(21935..23008)
                     /locus_tag="ACG9BE_001090"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFN3031852.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23064..23302)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23345..23578)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23655..24326)
                     /locus_tag="ACG9BE_001091"
     CDS             complement(23655..24326)
                     /locus_tag="ACG9BE_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3031853.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24413..27061)
                     /gene="mgtA"
                     /locus_tag="ACG9BE_001092"
     CDS             complement(24413..27061)
                     /gene="mgtA"
                     /locus_tag="ACG9BE_001092"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFN3031854.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27403..27577)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27750..28187
                     /gene="ndk"
                     /locus_tag="ACG9BE_001093"
     CDS             27750..28187
                     /gene="ndk"
                     /locus_tag="ACG9BE_001093"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFN3031855.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28209..29057)
                     /locus_tag="ACG9BE_001094"
     CDS             complement(28209..29057)
                     /locus_tag="ACG9BE_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN3031856.1"
                     /translation="MKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSFVTLRSF
                     LERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHFKKTEDL
                     YYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFPWIFSDR
                     FIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNEDMKRSLS
                     HKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSLEKKLLA
                     IAEPSN"
BASE COUNT         8387 a         6100 c         5163 g         9409 t
ORIGIN      
        1 ttaatttatt gcccgataat atatagaaat aaggtctagt tactgttttt tttaaatgat
       61 taataaataa caacttcttt ctatgaaatt gcaaagcatt catcttcttt tccgctaacg
      121 atatatccgt cagtgcttgg tacactttac taaaaagtat gagattgccg atatctggat
      181 aggtgtgcta tactacagaa aaagatatac aatctgccac cgggtaggat aaagaggatg
      241 atcattccat taggtctttg aagggatgat tttcctcttt ttgtgtattt ttttatagtt
      301 aaggaggaat gtaagtatgg catggatgga attaatcata gcaggaattt tggaagtatt
      361 ctggtcaacg atgatgaaat ggagtgatgg tttttctaaa atcaattatt ctttttacac
      421 agtgattggc atgattgcta gtttttactt tttgtcaaag gcaatcaaaa gtttgccgat
      481 gagtctctct tatcctatct ggacagggat tggcgcagtt ggttcggtca taattggtgt
      541 ggtcttgttt catgataagc tgaatctttc cacttggttc tttgtgggga tgctactcat
      601 cagtatcatt ggcatcaaga ttactagcgg ccactagaca cttggaataa gtactgatca
      661 gaaaaaataa gcctttttat ttaatattca atttttttat gaaaaaataa gaaataagtc
      721 caatgaccta gcgacttctc cactcatcag gtgtatgctt gtactatcta tagaaaagga
      781 gtgaatattt ttgaaaagtc tttcggtcca agaattatcc aagaactttg gtcacaaaca
      841 agccctcagc gatgtcagtt ttagcttatc ccctggcaaa atcgttggat tagtaggtcc
      901 taatggcgct ggaaaaacaa cgatcatgaa agctatttta ggactgatcc attactcaga
      961 tggaacaatc tgtattggtg aaaatgagat ttctccctct agtcataaag ggttggaaca
     1021 agtaggagca ttgatcgaat acccgggtat ctatccattc ttaacaggct atgatcatct
     1081 gaagttattc tctgaaacca acgatgtatc tgctatcgac actatcgtga atcagctcaa
     1141 gatggaaaag tacattcaca aaaaagcgaa atcctactcg ttgggaatga aacaaaaatt
     1201 gggaattgct cttgccttac tgaaccatcc gtctatcgtt attttagatg agccgatgaa
     1261 cggtttggat ccacaagcaa caagagatgt gcgaaaaatc attaccgact tggctactca
     1321 gggcacctct ttcttgatct ccagtcactt actcagcgag cttgaaaaaa tggtcgatga
     1381 gctgatcctg atcaatcaag gaaagatcat caaacaatgt acaatggctg aattgaacca
     1441 aacagcgcaa gatttcatcg ttatctctac aacaaatgat ctagctgctc gaacgatctt
     1501 gacagaagct ggatatactt tggaagatcc tgataaaatc aagcttttaa aagaaaacga
     1561 agaattatta ggaaccgtga tccagctatt gtctgcacaa aatatccatg tgacagacgt
     1621 ccagcatctg acgaatgatt tggaatcttc tctattggaa attctggatt cagtaaaggg
     1681 ggaagaagaa aaatgacatt gcttgtaaaa caagaattat ttaaattgat caagaaaaag
     1741 tcaactgctg ttttatctgt tctattagtt gttttgctca taggaacagc acttctagcc
     1801 aaaaaataca cgacgatcat tgatccagtt gaaatgactg ctcagctttt tagtgccaca
     1861 tcttggattg tctttatcat gattgcagct gcaagcacga tcatttcgat ggaagcacaa
     1921 tatggtacat tgaaaaatct cctttatcga aagtattctc gaggagaaat attagtaagc
     1981 aaatggatca cattagttat ttattctgtt tatttatacc ttttagctat tattgtcact
     2041 gtgttgatga aactgattct tttcccttcg atttccttca cggagaaggt ttctactggt
     2101 caaacactga tccaaagctt gttcacttat actttgggta gttatatcgg tttatggctg
     2161 attttaagtt tagtgttgat gattgcttgc tttatcaata gttccggcgc atccatctca
     2221 gcaggaatcg tcttttattt tgcttcgtca atcatatctg gtatccttat tgcactcatc
     2281 caaaaatggg agtggatcaa atggaatccg atcagtatgt tgaatctgca aaaccaaatt
     2341 ggaaacgaag agatcatgaa gcaattgact catctttcta ctaatcaaat gctattcggt
     2401 aatttagctt acatcgtttt gttcttagca ttaggatatc tcgtatttaa gcgaaaaaat
     2461 gtatgatgac tcatcaaaaa agaggctgtg aaagagacgt tcagccttat tgccgattgg
     2521 ctgtctctta aacaccgttt attctgaaaa aggagctgtg acaacatttg tgtcacagct
     2581 cctttcctag tatgtttttg aataaacgac aggctcacaa cctctcttta tacttctttt
     2641 atacttctat acgtgaagca tcccagtggc aaataaaacc attgcaatca ctccgaccat
     2701 cgtcacacct ataagtaaaa tcttatctaa gtggatatgt tgtacaggct cgttttgctc
     2761 cttacgtgct ttgaggtaaa aataaatgcc tggcaagaaa agaacaagaa caagcattac
     2821 ttcattaaag gctacgacta gcatagccca tacttgaaag gcacttgcta aaagccctac
     2881 taaaaattgg acccattctt tccgctgata agaaagtttc atttggtaca atcctaagaa
     2941 aagccacgtg aagaaaatag ctgctgtacc aagtgtgtaa gcaaagttat aagcactgtc
     3001 agtgaataat aatgaaagta aaaagacatt cgtacataac gtcgtcaata cgatagcata
     3061 agttggtgcc ccatttttgt tgagttttcc ccatgcttta ggcaaaagtc cgtcttcagc
     3121 catgtttccc attgtttcaa tcggtaagat cgtccatgag agccaaacac caactaaaga
     3181 aatgatcaaa ccgccgttga tcacgactgc tccccattta ccaaccatca tttcaaacac
     3241 atagcccata gcaggctgcg ccttgatcgt ttgcagctga ttcatcgtca ttgtcccata
     3301 tggaagcatg gaaaccaaga cgtaaataat aatcaagccg aatatcccta gaatcgttgc
     3361 tttcccaaca tctgattgtt tttttgctcg tgaacctacg acagaggctc cttcaatacc
     3421 aacaaatacc caaagcatca ccatgatgca actcaatact tgactggaaa acgggatcgg
     3481 ctgtccattc gcattcaata taatgtcgct agaaaagtta tcaatgaatt gttggatcga
     3541 aaacgtgctg atagctacta caataaataa aaataatggc actaatttac agaatgtgac
     3601 gatagcattg acgaaactag cattttcgac tccccgattc actagccatg catatcccca
     3661 taaaaagact gtactcatca cgatcgatgc cagattttgc ccaccttcaa atactgggaa
     3721 gaaatatcct aatgcgctca tcaacaatgt tgcaaaagct acgttgccta accagcatgt
     3781 gagccagtaa ccccaaccgc tgataaaacc agcgaaacgg ccaaaacctt cttctgcgta
     3841 actaaagatc ccgcctttta aatctggacg ttttttattg atattattga tcgacaagga
     3901 gagcatcaac attgctgttc ctacgagtac ccaactgaaa atagctgggc cgccagccga
     3961 tccggagatg tccgtcataa taccaaatac gccacctcca atcgctgaac caattactaa
     4021 ggcagcaagt ggaattaaac caattccttt tttctgttgc tctgtatcca tgtaagcatc
     4081 ctccaaaata aaaccgcttt tatcaaaaag tataacaaac cttttttatt tttacttaat
     4141 taataaaaac tttaatagaa atataattct accggctttt ttctcattat acaagcaaag
     4201 cgcctagtcc ctttccaaat tacaccacca aaaaacttaa aaaagatgct atactaaaag
     4261 gaaagaattg gaggatgatc catgaacatt ttatatatat cgatttcagg aaatacgcgt
     4321 gcctttgcca aacatctagc agaatatgct gaaaaaatgc atgcagaaga tcctgtcaat
     4381 cctgaagtta ctttaaaaga aatccatgaa aatagtgatt ttacgaaaga aacagaacca
     4441 ttttttactt tcgttcctac ttatttagac ggcggaaatg gtttagataa cggtgataca
     4501 gaaatcttga ccgaaacgat gcgtgaatat ctggaacatg aagacaatca taaactttgt
     4561 cttggtgtag tcggtagcgg aaataaaaac ttcaataatc agtattgtct aacagccaaa
     4621 caatatgcac aaacatttgg ctttcctttc ttggcagatt atgaattacg gggaacacca
     4681 tctgatgtga cacgaattta tcagatttta gcagaaaaca ataaataata aaaaagaaga
     4741 agctgaaaaa tagatttcag tttcttcttt cgtttctatt gtttctatca tattaagctt
     4801 tgacatccat cacaggtgct tttgcttttg ctttacctgt tttttccaag acaaagtttt
     4861 ttacacgatc catattggtt accaagacga tcatggatgt ttctttgcct gctgctttga
     4921 tcgcttctaa gtcagcttct gctacaagcg tatcaggtgt cacttgctgc ccttctgtta
     4981 ctttcacatc aaatggcgta ccgcctaagt caactgtgtt gattcccata tgaagcaaga
     5041 tttctaaacc atttggcgtt gtgatcccaa tagcgtgttt cgttgggaag acagagctga
     5101 ctaccccagt aactggagaa tagatatgtc cgttttctgg ttctacaaag tagccttcac
     5161 ccatcatctt ttgtgcaaaa actggatcac ttgctttatc taaagaaaca actgttccgt
     5221 cagcaactga tgagaggata gtcacacttt cagctgaagt ttgaggtact actttttcag
     5281 gcacttcatt tactgtggct gtttcagtcg ctaattcttc tttttctaaa cggtctttag
     5341 gaataccgaa gaagaatgta gcaacgaagc caccagcata agctgccaat aatccaagga
     5401 tgtagcctaa ccatttaccg tcagagatca atgggatcaa agcggctcct gaaggtccaa
     5461 tagcgatagc cccgacattt ccgatcattc cgatcactgc cccgccgata cctccaccga
     5521 tacaagcagt aataaatggg cgtcctagag gtaatgttac tccgtaaatc aatggttcgc
     5581 cgattcctag gatcccgact ggtaaagctc ctttgatcat ttctgttaat tctttatctt
     5641 ttttgcagcg aatccataat gccaatgctg cacccacttg tcctgcacca gccatcgcaa
     5701 gaattggaag caatagcgta cgaccagttt catcaatcat ttgcacatgg attggagtca
     5761 aaatttgatg cagtccgaac ataaccatcg gcaagaataa tgtgcctaga acgaagccgg
     5821 caaatgctcc accaacgcca agtacccagt tgatcccacc gaccatgcca tcagaaatga
     5881 aacctgctaa aggcatgatg aagaaaattt caatcagacc gatggcaatc aatgaaagag
     5941 ttggtgtaac gatgatatct accgcatcag gtactacttt atgcagtttt ttctcaacga
     6001 ttgacaatag ccaaacagcg aagatgacac ctaggatccc accttgtcca gctgcaagag
     6061 cttcaccagt aaataagttt ttcagtggtg cttccggatt catccctgtc agcatcacaa
     6121 cggccccgat aactccacca agagttggtg ttgcaccaaa aacttgtgca gcattgatac
     6181 ctgtatagat aactaggtag ctaaacatcc cgtttttcaa gatgttcatc acatcgatat
     6241 attgttgcca tgttgctgca tcaagtgttc cggctgtgac taagtttgct aaaactgcgg
     6301 caatccctgc cacgatccca gtaccgacaa atgcaggaat cattggcaca aagatattag
     6361 aaatgtcttt taataccgat ttgattttag atggtttttg tttcgatttt tgtgccgctt
     6421 tcatttcttg tgcccgttcg tttacacggt cttttccaga tgctgatgaa tttgctgtag
     6481 ctgtacttac agaagaaagt ttttctccta atttcacgcc tgccaaatca accatttgct
     6541 gtgctacttt gttcactttc ccaggtccga tgatgacttg aagctgttca tcttctacta
     6601 caccaagcac tcctggaatc gcttttaatt cagagacatt tacaagatca tcattgatga
     6661 tcttcatacg cacacgagtc atgcagtgga caatgctttc tacattgccc atgccgcctg
     6721 cccctttata aatttgtttt gccagttctt ccaaagtcag ttcgtttttt gccatggtct
     6781 tttccccctc ataaggtccc ttttacaaat ccgtttgctt cagctaattt tgcttgagct
     6841 tcctctttat cactatttgt caaaatcatg acgatagcca gcttaacatt ttgatctgct
     6901 tcttcaaagt acttttcagc tgtttcgtaa tcagcttctg tcgcttgcat gatgattcgt
     6961 tttgagcgtt cgattagttt ttcatttgtt ggtttgacat cgaccatcaa gttattgaat
     7021 actttaccta taccaatcat actgatagta gaaatcatat tcaagataag tttttgtgct
     7081 gttcctgact tcaatcgagt agagcctgtc aaaaattctg gacctgcatc gacttcgatt
     7141 gggaaatccg cgtgtttact gatttcggct tgtttattgc aagaaatcgt tcctgtgaca
     7201 gcaccgatcg attttgcgta atctaatcca ccgatcacat atggtgtacg accgctagct
     7261 gcgataccaa taactatatc attttttgtc aaattcagct tgattagatc ttcttgccct
     7321 agctcataag aatcttcggc cccttcgaca gctacagtca ttgcttcttc tccaccagca
     7381 attagaccta tgaccatttc cggttctaca ccgaatgtcg gtacgcattc agcagcatct
     7441 aacacaccta gtcttccact tgttccggct cccatataaa tcagacgtcc acctttttta
     7501 aaggactcaa tcgtgttagc aatcactggt tcgatacttg tcagttcttt ttcaaccgca
     7561 gcggccactt tctgatcttc tttgttcatt agttgaacgg cttgagaaat actcatctca
     7621 tctaatccaa aagtttcttg gtttctgcgt tccgttgtta ggttctctaa attgattttg
     7681 ctcattttta tcttccttct tttcttataa attgatagtt cataccagct ttgatttggt
     7741 tgatcaatgg tagctcttct ttgataactt tagctactac attcactttt tcatccgctg
     7801 gcaaatcata tttggttaat tggatctcac ccatatagcg caaatacttt tcgttatcta
     7861 aagtaataga ccctttcata cgcgtagctg tatttcttgc tgggataggc ggaatctctc
     7921 gaaaacgtgc atctacgctt cgtatgacat ctcttgcatc gtcttgccga ttcgtatgct
     7981 tccctaatat atattcgtaa aattcttcat ctacaggttc cgtatggagc aagactgtct
     8041 gttctttttg gaaagaagca aattgctcct gaacgttttc tgacagacct gcatctccga
     8101 tgtaaacaat atccgtgtta cttgctaata gatctagagc gcctgcaaga ggatggatcc
     8161 ctcgatgttc ttccaaagtt ggcaatcctt gatatagtgg gcctctcaac tctgtatctc
     8221 ctggtacaaa accttggaca aggagacctt gaattttgag ccattgattt tttttctgat
     8281 accattcttt gtccagccct gtttcgggac gtggataata attatgccaa gcttcaatct
     8341 gtgagaaatc agcttctgct tctttcaatt catcgatatc tttaggcgtg atcgtgctag
     8401 cattcaaact gattttcaat tgatgtgacc agtcggcaat ctgctgattg gaaatatgat
     8461 aatccattct taaacctgtt acaccaattg ctttcaaagg cgttagatct tctaatgaaa
     8521 aacccgctcg ttccaatgcc tctcctgaaa tatcgaccat caattctagt ttattctttt
     8581 tcgcttcgct tcctagttcg atcaatcgct tttgatatgc cgaaacatca tcttctggta
     8641 tatgcatgga agtgaaaata ccgacaaagc cattggatgc catttggcgg atatactgtt
     8701 tcttctcttc tgacaaagaa tcgttcataa atattgaaaa gccaaacata ttatccctct
     8761 ttttctgtat ccgtcttcat ttctatgact agagtatata tctaaagaaa caaaatttca
     8821 atacttttat taaaataatt aaatttcatt tctaaaggtt gagtttatat tatcaaaaga
     8881 attgtcataa cctaaaaaag gcactgctaa gcaaaagcag tgccttgaaa tattttattt
     8941 aaataacaca aaaacataat tactcataag aaaccaattc tgtattatct accatgaatg
     9001 taaagaaccc ctcattaggc atcggatgaa aataagcatt gatcactctt atcaaggcat
     9061 tatggcaaac taacagatag acattttctt cgtcttcgat gcattctttc aataaaggaa
     9121 cgatccgtgc atacacatct aagactgact ccccatttgg gaatcgtaca gcgaacgcta
     9181 aacgtgcttt ttgaaagttc tcatctttgc ttggcagtcc atcataatcc ccaaaatcca
     9241 tttctaccaa tcgttcatca acggtcagcg gcaagctgag acgatcagca actgcttgtg
     9301 ctgtatctct tgctcgttga agaggggaat ggatgatttt cgtgattgga acaggcaaat
     9361 ctactacctt ttctgctagt ttctgtgcct gcatctgtcc tttttcagtc agcggcacat
     9421 ctgcccgtcc gcaaactaat ccttgtgcgt tccaagttgt ttctccgtgt ctcgttacat
     9481 aaagcatgtg attctctcct tcgttggcaa agttcccctt aaccatacct tgttgttctt
     9541 gagattacaa gggttagatt ctctcaagag aaataatctc agtagatact ttatccagga
     9601 aggtttgatc atgttccact agtaacatgg caggtttcac gttcaagatc agttgttcta
     9661 attgttcttg attgaagaca tccaaataat tcaatggttc atcccatgta tatagttcag
     9721 ctggctgtga taaagattta gccaattcca cttttttccg ttggcccata ctcatctgct
     9781 cgatcttgtt atgaaaaaca tctctttcca tcccaagctt tcggaggttg ttcaaaaatg
     9841 cttggtagtc tacttggttt ttctctgcaa attccgccaa cgttcctcga ttgtcttcat
     9901 aattttggct tgcataacta atgctcagat gttttggctg gtatttttct cctataacct
     9961 tgccgttgaa tgcccccaga agataatgga taatggatga ctttcctgca ccgttaggac
    10021 ctgaaatcgc cacccgctga tgaggctcga ttgtaaaatg aattggctcg aataacagat
    10081 tttcataccc taattgaaga tcttctacgc ttaaaagtcg cttatggtga gacgcttggc
    10141 tattcatcgt caaagaatcg atatattcga tatcttttaa tagtttttct ttctcgctga
    10201 tctgggtctc catccgattc acgattgctt tggatcgttt catcgtccgt gcagcatcag
    10261 cacccactgc tcctttattc actcgtctag attcagtatc gatgaatccg acttgtttct
    10321 ttgttttatc tccttctcgg gaacgagacc attcggcttt ttcagctgct gttttcttta
    10381 gcctactgac ttcttttttc aatttttcat tttgagccat ttcaaattca tcacgaagtt
    10441 ttttctgttc ttcatagata gagaaattcc cttgataaag ttccagttga cttttttcga
    10501 ttgctaaaac atggtccact acttcatcga taaatccccg gtcatggctg accacgatga
    10561 agccttgttt tttctttttc aaataagccg ctacttgttt tctaccagag atatccaaat
    10621 gattcgttgg ttcatcgatt aacgggaaat gagtgtcatc cacaaataaa agtgccagta
    10681 ggacttttgt cttctctccc cccgatagtg tgctgaattc tctccataag atttcaggat
    10741 ctgtttgcat caattggagc tctctttcga tttcccatat ctcaaaatcc gtaatatcat
    10801 ttaacacgta ataggttaaa cgttctttat cttttgtctg ttgcgggaaa taggcaaatt
    10861 cttgctgatg gattacttgc ccttgataag gtagtttgtt ttgtagaata ttcagtaaag
    10921 ttgtctttcc tcgaccgttt cgtccgataa gtcctagttt ccattgtgtg tcaaaattta
    10981 gatttgcttg ttcaaataat aatgtgcctt ggctgtcgta gccgaatgtc agatttttta
    11041 tttcgatttt agacattttc catcacctcg taaaaaaaga ccagtcctat tttcagaact
    11101 ggctaaagag gatgcaaggg ttggatacac acaaaaagac ccatacatcg aaaaaaccaa
    11161 gccaatcccg aaaatctgca cactgcttcc ttgtctccac aaggtacagt ctgaatgcag
    11221 ctttcatcaa tcgacttaat ttttcaatgc acggatccct cactttgtcc tatatttgaa
    11281 attacgatag cataaagaaa atgattttgt caactacttc gggaggatgg tgaaaaatct
    11341 gctaagtgtc tgtcctaaga tttttgtttt ttctaatgtt tgcctgtgtt ccattgcttc
    11401 ttctaaacct attggaccca aatgaatact taagacaatt gggagaaaat cagaaagtcc
    11461 ttctagctct tggtctctac ttccgcaaat agcgattatt ggttttcgat atttttttgc
    11521 aagcactgct acaccgtatg gaacttttcc gtggatcgtt tgctgatcga tccgcccttc
    11581 tcctgtaatg atgacatctg cttgttcgat ctttttttcc aatccagtga tttggctgac
    11641 taaatcgaaa ccagaaacca tcttcgctcc caacaaaaaa agggctccac caataccgcc
    11701 tgctgctcca cttcctggaa tatcatctaa atccacctcc cagaaatctt ttatttgctg
    11761 agcaatcttt cctgcttgtt tatctaagaa agctagcgtc gtatctattc ctcctttttg
    11821 tcttccaaat tgccaagcag ctccttgttg acctgtatat ggattggtaa catctgcagc
    11881 gataatcaat tttactttag aaacaagaac gtttcttcga tcaaaatgaa gttttttagt
    11941 agataataaa ggatttcctt ctgtgtaccc ttctaaagaa gcacccaagg attggagtaa
    12001 tcctagacca ccatcgctac acccgcttcc tcctaaagtg acaataattt gttctacttc
    12061 ttgaagcagt gcatctttaa taagttctcc taaggcataa ctggaagcaa gtcggatagt
    12121 ttgatccgtt ggtttatcaa gtagatctaa ccccaatgct tgtgcagatt cgatgactgc
    12181 ggtgtttttt ccttgccaag ttgtcaataa atagctgatt tttcgctgac ggaataagag
    12241 atccatccca tcaatggtac gccatgttcc cctgagcgcg taatagattg cttctgcagt
    12301 attttctccg ccatctgcaa tcggcacatt gatttttctt cctttacgat cttcccagcc
    12361 atttagaact gcctgattta gttcaaatga agaagcactt cctttaaatg aatcaattgc
    12421 cgccaaaata tccatctgta tccctcactt tcttcttaag taagacactt tttttcattt
    12481 ttttcaaaca aaacaatgct attttcttcc atttttctat atactaaaag ggaactcatt
    12541 gaataaagga gagaaaaaac atgaaaatcg caatagctgg ggccggagca atgggcagcc
    12601 gaatcggatt gatgctacat caaagcggaa atgaggtcct cttgatcgat cgttggcctg
    12661 ctcacattga agccattcgt acaaatgggt tgattgctga ctttaacggt aaagaagtgg
    12721 tcgcaaaact gccaatctat tcaccagaag aaatcatcga aagcaacgaa cacgtggact
    12781 tgatcgtagc attaacaaaa gccaatcaat tagatgatat gttttgttcg atccaatcca
    12841 ttattactga caatacttat gttttgtgtt tactaaatgg attaggtcat gaagatgtac
    12901 tggaaaaata tgtgccaaag aaaaatatct tatttgggat cacgatgtgg acagctggtc
    12961 tagctggtcc cggaaaagtc acattgttag gtgatggaga aatcgaacta gagaacttgg
    13021 aaccagaagg cgaagcattt acaaaaaaag ttgtagaagt ctttcaagaa gcaaatttaa
    13081 atccgatcta tagccataac gttcgttatt ccatttggcg aaaagcttgt gtaaatggaa
    13141 cattgaatgg tttatgcacg attttggatt gtaatattgc tgaactaggc gcgcaaaaag
    13201 cggcagagag catggttcga acaatcgtat ctgaatttgc ttctatcgca gcaaaagaag
    13261 gaatcatact agatcaagaa gaagtgtacc aacatattgc ttctacttat gatcctgaca
    13321 acatcgggtt gcattaccca tctatgtacc aagacctgat caaaaaccat cgtttgactg
    13381 aaatcgacta catcaatggt gccatttggc gaaaaggaca aaaatatgat attgctacac
    13441 catattgtgc ctttttgacc caattagtcc atgcaaaaga aggaatcctt ggcgcagaat
    13501 aaaaggctct ctgatgagaa tcagctgaat cagcagctgg ttctcttttt ttaagcccaa
    13561 taaaagatca aaaagaaaac acaactctca tgatgccata agaattgtgc ttgtttgttt
    13621 cagtacataa tctcttcaaa atgtaggttt tttatttgtt ttcaacagag gagaggtcat
    13681 attcgtattg ctcataaata gtggaatcgt tagtgataaa tcgttttgcc caattttctg
    13741 catcataaaa ttccaatagc tctgatagga ttggcttaaa tggtgtatgc agaaattccg
    13801 gtaattcatc taaatcacgt tctcgaagca aaaaaatatt ttcaggacga taaaaatgat
    13861 aatgagcaat cgcttgatta tccgtcacta gtttcttttg aaaatacatg gattcaaatg
    13921 tccgcaaagt cactccttgc tgaccctccc gcaacagttc cagaatcccg cgcgattccc
    13981 ctgttttttt caggtactct tcataagtaa gatatccagg ttgttcattc cctcgatgtg
    14041 gaagaatgaa atagttggtc gtaacatctc tttttataaa ttcctgctta tagctttctg
    14101 ctcgttcttt tctcccatta ttcgttgcac caaaaaatag atccgtcttt ggttcgctag
    14161 aagaaaaatt catatgtttc gaataaaatg aactattatg tttcagccca agttcttttg
    14221 cttccaaaaa atcaaaatga taaatctcat cgattgccgg ttcatcttgt gcctgcttca
    14281 gcatcgcctc gtgttctgct ttgaagtgat tccaaaaaaa taacacaatt tttgcattgg
    14341 taaatgggcg cacgcgtcga gccatctcca tatcaaatcc ataatctaaa aaaacgatca
    14401 gctgataatt tgccaactgt tgtctagctg atttaatgaa ttccgactgt agtaaatctt
    14461 ttccggacca aacatctaaa gagcggatgg aaggcaaaaa ataaagtgat ttattttttg
    14521 caactagcag tacgtcattt ttgttcatct tttccgcctc actttctctg tcactttttc
    14581 ctatgatagc ctgtttaaat agtaaaaagc cagtaaaacg tttcctatat ttgtatgaaa
    14641 atttaacatt tgtatgaaaa tatgatgaaa taaatcattc ttctattaaa attcgcctaa
    14701 aagttttctg aatagttgta attgtctgaa aatttgttat cattagtcat tgttaaatat
    14761 tgctatttat tcttaatata aattttgatt gtgaatttaa aagaaagagg tcatttatgg
    14821 aagataaaaa attgaaaaaa caacttagct ccagacatat caccatgtta gctttaggtg
    14881 gagcaatcgg tgcaggtcta tttaaaggaa gtggcgaagc gattgggatc gccggccctt
    14941 ctgtattgat tgcttttttg attggtgggc tgattttata tatcgtgatg aaaggtctgg
    15001 gaaaaatcgt tttaagcggt ggcgacacgc atcacggatt atctggtttg atacgccctt
    15061 atctaggtgc tcattctgct gattttactg attgggtcta ctggtctatg tggattatca
    15121 atatcatcgc tgaagctgtt gcagctgctt cctttttgca gttgtggttc ccgcacattc
    15181 cagcctgggt atttgttttt atgttggctg ttttaactac gatcatcaat ctttattcgg
    15241 tacggttatt cgcagaaaca gaatattggc tggcttttgc taaaatcagt gtcattatct
    15301 tactgatcat ttttgctact tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc
    15361 ctacccttca gcaattgact gaccatggtg ggtttactcc tcatggtatg aagggaatca
    15421 tcagttcttt gcttgtagtt atctattctt acggcggttc tgaactgatt gctatcacgg
    15481 taagcgaagc agatgatcct aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa
    15541 gaatcatttc cttttacatc attcctttgt ttttgctttt gattcttttt ccttggaata
    15601 ctttagcagg cacgaatgtc agtccttttg tgatggtatt tgaaaaaatg aatatcccat
    15661 ttgcagcgga tatcgtcaat tttgtgattg tccttgcttt gttctcatct atcaattcgg
    15721 gtgtgtatgc atcttcaaga attttgttct tccgtttgaa ggatcgaaaa ggttcctcta
    15781 gaaaactagc tgttttaaac aagcatcaag taccgcaacg tgctgtttta ttttgtacca
    15841 gtgttcttta tcttggtgta gctctttctt attttgtcgg tgacaaatta tttggctatc
    15901 tggcaggttc cctttcgtat acagtattat tgatctggat cattatcagt gcggcatcct
    15961 ttgtactggc actaagaaaa ggaacattat gggataaagg aatcagcttt tttgcattag
    16021 ctgtcttagg attgattttc cttggtatcc tgttcacgaa ctccattggg gtaactgtat
    16081 tgactggcct gctttacctg ttcatttatt tcagttatca aaaaaagaat gatgcgttcg
    16141 tattgacgaa tgaatgaaat cgatagaaaa ttttatttta tttatgtgga atagatggag
    16201 ctattgaaga aaggagttgt gacaaaagtc ttgttgcaac tcctttttcc gaataaacag
    16261 tctcatgacc tcttggtaaa cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc
    16321 tccatgctca tgacatgata gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa
    16381 aagtattgga agtttttgtt gatgatcggt gtttcaacgg tagttatgtt tggtatgatg
    16441 tacttgaatg tctatgcgct cgatcatatc ttcttcagtc gtacacgagt attcatggcg
    16501 ttgatgatgg gtgcagtcat ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa
    16561 aacaaaaaga taaatgttat gatcttagcc gctagtcttc ttgtttttgc cggttccttg
    16621 tttatggtac gaagccaaac tactgtcaac gacacagctt ggatgaaggc catgattcct
    16681 catcattcta tcgctatcct ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa
    16741 aaattagcgg aagaaatcat tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg
    16801 atcaaagaaa tggaatgacc attatccgtc caaataaaaa aagaggatgt tccagaagtt
    16861 atcagcttca agaaataaga agaagctact tctggaacgc cgtgaataag gggttgtgaa
    16921 aaagctgccc tgcatcaagt aataagaaca gccaaacaaa aatagcttct cctatttttc
    16981 gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt aagaggttgt
    17041 aaaatcagcg ttctgacctg agtattagag agacaaatgg aaaaatagtt cctcatattt
    17101 tgccattttt tgagctaata ccgagggtca gctgattgaa caccgtttat cgagaggttg
    17161 tgaaaaagtc gcttagtttc gcgtattaag aaaaattttg tgaaatggct ttccacattt
    17221 ttgtataaat ttggcttaat accgagaaac tggcttttga ataccgttta ttcgtaaaag
    17281 ggattgtgac aaaagtcttg tcacaatccc tttttatgtt tttattgttc taattgcgct
    17341 gtcttgctta ctgcttcttg cggtttcaca aatttttttg agattcccca acctgtgaag
    17401 gcactgacca tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg caagtaagtg
    17461 agtgtaggaa taccaaatgt acttgctacg aaacttccgg caattcccca aggcactaag
    17521 tagttgatta ctgttccacc atcttctagc gttctaccta atacagttgg atcaaatcct
    17581 gctttttcat aagtttcttt aaatgcatta ccaggcagaa taacagatag gaattgttca
    17641 ccaataaaaa tatttactcc gatacttgtc atcaatgttg taaaaatcaa atttggtgtg
    17701 ttcgtcatct ttcttgatac tcgttccatc acggttgaga tcaagccaaa ttcgatcaac
    17761 aatccaccta atgacaaagt tagaataatc aatgacacag taggcatcat actttcgatt
    17821 cctccgcggc taagcagtga atcgatttgt tggtttccag tatttgaaac aaaccctgat
    17881 tcgatgaccc cagccaactt cgttaaggaa gtttgtgggt tttgaataaa aatcattcct
    17941 actgctacag cgatattcaa caaaatcgtt aaaattgccg gcaccttttt ccatgcacac
    18001 agtaacatta aaaataaagg aatcagtgac cagattgaaa tagtaaagtt tgcttctaga
    18061 acatcagtca cttcacgaat agatgaaaga cttgcggctt tacttgtgtg tcctaaaatc
    18121 atgaataaaa agaaagaaac aacaaatgcc gggacagttg accacatcat atgcttgatg
    18181 tgtttgaaca aatcggcatc aacaatagct gctgctagat ttgttgattc ggataatgga
    18241 gacatcttat ctccaaaaac ggcacctgaa acaatagctc caacaaccag cgctggattt
    18301 aagcctagtg ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat aacagtaaag
    18361 gcactcccta cagcgctacc tacgattgca cacacaacaa atacagaagg gacaaaccac
    18421 tggacactga tcaatttgaa cccaatgacc attagagttg gaatgatccc tgcttgtatc
    18481 catacagcaa tcaatgaacc gactaaaata aaaataaaga ttgggatgat tcctggtttg
    18541 atgccattga tgattccttc gtgcagccaa tctgtcgggt atctttttat cactgcatac
    18601 aacatgacca ttccgatcgc tgctaagatc gtgatattag gtgagacctt taatccgaca
    18661 acgccaatcg caatgcctaa aacaataacc attaatacaa gtgttgcttc taaaaaactt
    18721 ggtgttcctt tttttcccat gataatctct ccttttttag ttgttcttat gttctaaaaa
    18781 agttaagata tcggattact ccgccgcaat tcatccgcga ttcctccttt ataaaaataa
    18841 aaagacctgt tttaaactaa ggacgaatga ctccgcggta ccaccttaat taaaaacaga
    18901 tctgtttttc actttcaaac ttttattcaa atgtatggac tttccatgta attcgtcaat
    18961 taggcttgat cggttcacag cgaccaccga ctttctgaac aaaacaccta accgctactg
    19021 cgtgaaaagt tgacttgtac gaagtttacc ttccataaga tcatttgtca atgaaaaaga
    19081 aaatattttt tcatttttct gaaaattatt tttataactt tcacacacaa gtttattttt
    19141 tactagaaag tagcgccctc ttgttttttt cttgctacta tttcttgatg ttcttgcttg
    19201 attctttcta atagctttgg ctctttcaac agatcaagag ctgtattggc gagtagttct
    19261 gctgcaatcg caatagaagc aaggcctttg tcacttttag cagcagcttt gaattcttct
    19321 gtgtgtccgg caatatatgt ctcagagatt gctaccgtag gctggattgt tggtatgact
    19381 tggctgacat ttcctacatc agaagaaccg agacttgttc tttgttccgt ttctatttct
    19441 tcatctggaa cacctgcttc ttttgcatgc tggaagaata agtcatcaaa agaaggagta
    19501 acgatgacat catcaactgc attttggaac aatccaaact caaaagtcgt gcccgtactt
    19561 atcgcagcac ctcgtacgat attttccact ttttcatata ctttatctaa tgtttgccga
    19621 tttcctgcac gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt ggcagctact
    19681 cctccgtctg taatgatccc atggatattg acatcttttg gcaattgcaa gcgtagagcg
    19741 ttgattccat taaacacttg gatcaatgct tccaaagcat ttactccttt ttcaggtgct
    19801 gctgctgcat gggaagcgac accgtaaaat ttgatgtcga ctggatcatt cgctaaagat
    19861 tctgtcgttt tcccataacg aaaagctgga tggacgcata acgctacatc gacatcctca
    19921 aaaaaacctt ctcgaacaaa gctgccttta gctgatccgt tctcgccccc ttcttcccct
    19981 ggggtgccat atacacggat ttctccacca gtttcatcaa tgaattgctt cactatgctg
    20041 gcggctaata cggagtaagt accaaacaaa ttatgcccgc atgcatgacc aatacctggt
    20101 aaagcatcga attctgccaa aaaggcaatt gtgggaccgg gtttattgga aatataacgt
    20161 gcatcaaagg ctgttcggtg tccagctact tctttttcta cccaaaacct ttctttttct
    20221 aattgtttaa tcaacgctgc tgaggaataa aactcatagt tacttacttc aggatgttcg
    20281 tggatatcta gagcaagtgc ttgatattct ggtaaacgat attggattag atccttcaat
    20341 tggtttgttg agattgttgt tgtcataaaa aattcctcct atgctagtca tttttcttat
    20401 tttctcccga atgtttccca tgcaggcacg cttgatcctt ttgatgtttt ctcgattact
    20461 ttgactgtgt cttctgtttg ataagcgtct actatttttt gatacgtttt attgttttca
    20521 tcttcttttc ttgcaacgat gatattcaca taaggacgtg catggtcatc cactggttct
    20581 aagaagatgg catcagaagc aggattaaat ccagcatcta cagctacacc gctattgatc
    20641 actgaaatgt ctacatctga caaagcacgt gctgtttggg aagcatctaa ttcagaaatg
    20701 tctagatttt ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc ttttgcctta
    20761 tccactttga tcaatccagc agtttgcaat aacagaagag ctctgccgcc atttgtaaca
    20821 tcattaggga ttgctacttt tgctccgtct ttgatatcag ctacatcctt caaagtgtta
    20881 gaataaattc ccagcggtgc attgacagta tttccgatcg atactaaatc agttccgtgt
    20941 tctttattgt aattgtctaa aaagaattga tgttggaaag aattcaaatc aatcgatcca
    21001 tctgctaaag ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc caaattaatg
    21061 ttctcctttt tcaaacggtc tttcacgttg tcccatacat ctgtatcttc tccaataatc
    21121 cctagtttta cgatagtttc ttttttgtca gttgtatctg cttttccttg gctacaccct
    21181 gccaaccaaa taattgctac aaatgtaatg actacaaaac gaataatttt tttcattttt
    21241 tctcctccac tttcaatgtg taattttttt aatcagttga ttactgataa attgacttaa
    21301 aaaaactaag attagaatca ataatgtagc cacaatcgtt acatctgttt ggaatcgatt
    21361 ataccctctc gatatagcaa gattccctaa tccgcctgca cctactgctc cagccatggt
    21421 agtcagtccg atcacgttga tgatagtcaa cgccgagacc cgaatgatac caggcaaacc
    21481 ttctaacaaa tacacacgta aaatgatacc gattggactg gttcccatcg cttctgccgc
    21541 ttcgatcacc ccaggatcaa cttctagcag tgcgttctcg atttgacgag caaaaaacgg
    21601 aacaactcca aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg ttccaactaa
    21661 gaaacgagta gtcaatgcta aaagagaaag caagataatg aaagggatgg aacgaatgat
    21721 attgatcagc ttatcaagta tttcaaatag cagacggtta gctagtaatc cattagggcg
    21781 tgtcactact aacgtcacac ctaagatgat tccaatcatc ccgccaatga atgctgtcca
    21841 aaaagacata tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa tcaaataaac
    21901 attgggcaaa tatgttttaa agaaatcagt cattttatct tcctccttcg atcactttga
    21961 tacgactatc taaatcgttt ttttgtaaaa tatttatttt tacaccagat ttgatcaaat
    22021 aatccaatgc ttttttccgc tgtttggttt ctccagctaa agtaacgatc aaactcccta
    22081 acggcgtttc ttgtaataac tctacattgc catataaaat attcgctgtt acttgatatt
    22141 tgctgtacag atgagcgatt agaggttcgt tcgtctgatt tccaatataa cttaattcca
    22201 ccagccattt attggcgatc tgatcggcaa aagcagaatg ggcaatgatc gtttctaatg
    22261 cttggtcaag atgagtagct gttcgaataa aatctttggt caattcttct tcaggattgc
    22321 tgaagatctg gatgctgcta cctttttcaa cgattcttcc atcttccata accgctacct
    22381 tgttgcagat ttctttgaca acttgcatct catgagtgat caggacgatt gttaaaccta
    22441 actggcgatt caattttttt aacaatgcaa ggatctgaag ggtcgttttc ggatcaagtg
    22501 cactggttgc ttcatcacat aataatacct taggttcatt cgccaaagct cgtgcgattg
    22561 ctactcgttg tttttgtccc cccgacagtt gtttaggata agcagatgct ttttcttcca
    22621 aaccgacaag ttccagtagt tcattcactt tttgacgacg ttcctgtttc gattttccag
    22681 aatactttaa agaaaaatca acgttgtcaa aaatagttcg gctttccatt agattgaaat
    22741 gctgaaagat cataccgatg gcctttcttt cttttcgcaa ttctttggga gagagctgac
    22801 tcagattcgt ctgattgaca atgacttctc ctgctgttgg tttctgcagc aaattcatca
    22861 cacgaactaa cgtacttttc cctgcacctg agtaaccgac gattccataa acatcgcctt
    22921 tttcaatcgt taaatctaca gaatctactg cctgtatctc tttttctttt tgtttgaaac
    22981 atacagaaac atctcgcaat tcaatcatta aaattcctcc ttatctaaaa aaagcgtcct
    23041 caagaaaatg aaaatgtcat tttcttgagg acgctttttt gcgtgttacc acctcatgtt
    23101 caagctgtgt tcacacacaa ctcctcttca agtacgatca tactctagca agatatcggt
    23161 cgcagccgtc atcgccttac atctatgctc gggatgaatt gctcgatggc cattttccaa
    23221 tagtcgttcc ttgcttcttc tcagctaccg aagctctctg tcaaaggttc ttctatcgta
    23281 cttaccattt caatgcaaat aatattcaaa aaaaaagtcc ttttactaac ataaactatg
    23341 tcagtaaaag gacgacgtat cgcggtacca ccttttttcg gaagtttcct tccctcaagc
    23401 ccaacatctc aaaaagagag attggcgttc gttttaacgg tcgaacagtt ccgaatttgc
    23461 ctacttatcg acaaattatt ctccaaggcc attttcagct tttcttactt gactcttctc
    23521 accagccaga gcctctctgc aaagtttcca aacttacttt ccttttcaac gaaatcgata
    23581 ttttgttgtt gggattaatc tacatgagga aaactgaaaa gtcaagtacg aaattcaaat
    23641 tattttttct atatttattt ttctgttttt ttatttaaca tttcaataac agttcgaaga
    23701 tcttttcctt ccgcaagaag tttcaatttt tccttttctt cgacggtata atttcggaca
    23761 aaactggaat caggtatcaa aacaacgcct aatcctgctc gtgaagcagc tgttgctcct
    23821 actaaagaat cttccactgc aaaggctgaa ctagcttcta atcctgttcg ttccaaagct
    23881 gtcagataag gaatcggtga aggcttgttc tccgacactt cgtctccgaa tgtaaacgtg
    23941 tccaagtaat cagctaattt aaagtgatcc aagatatctg ttgctctttt cgtaacagtg
    24001 gtcgtagcaa tgcctagttt catatgacgt tcttttaagt ctgccaatat ttcttttgca
    24061 tatggtttta agtggatggc tccgcttgtc agctgttgat aaaaatattc ttcacgcttc
    24121 gctcggactt cctgtacttt ttcagggcta cctgctgctt tcactaaaat atcaaaactt
    24181 tgtttccagt tttttccgcc ccaagcgtca acgatttctt ttgggatatg gatatcaaat
    24241 tgctgaaatc cccaaagcca gccatcacga tacgtttttt ctgtgtcaac taacaaacca
    24301 tctaaatcaa atattgcacc tttcatctat acattaatcc tttccaaaac aatacacata
    24361 gtttagcacg actggtccaa aaagaacaga agcaaagaac cagccttctc gtttataaga
    24421 gttcttggaa tcgacggata tagattttct tcacagctgt cacaagcgcc agataagcga
    24481 tgactgtagc aaataaccat aaccagtaat tgccaggaag tggcatcaag cctaattcag
    24541 caccaaaact tgtaaaagga aggacagaac cgacaataat tcctaatgta gtgataacaa
    24601 acatggcaaa tgttgcgttg ctttggataa atggtacttt cggtgtacgc agtgcatgga
    24661 gcactaaggt ttgtgtccat agtgattcga caaaccatcc tgcatggaat aatgcgatga
    24721 aaatcactcg ctgtgaacta tccaatgtat ggaaacttcc gcccactaca gcaggacaaa
    24781 taacaaaata catcaacaag taagtagtaa tatcaaatat cgaacttgtt ggaccaaagt
    24841 aaaccatgaa ttccccaata ctagatgcat tccatttttt tggctcgtcc aaatattctt
    24901 tatccatccg atcccatggc aaggaaatgc aggaaacatc atagatcaga ttcaaaaaca
    24961 aaagttgtaa aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc agcacggaaa
    25021 acatattgcc aaaattagaa cttgctgtag ccttgatata tttcatgata ttcccaaaag
    25081 tttctcgtcc agaaagaagg cctctttcca aaatcgtcaa atctttttct aaaaggatca
    25141 cgtctgcaga ttctttcgca atatccacag ctgtatctac agaaataccg atatcagcca
    25201 ctttcattgc aggagcgtca ttgataccat ctcctaaaaa tccgacagta tgaccatttt
    25261 tacgtaaagc tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg aatacttcat
    25321 ggtcttcagc tacttgtgct aattcatgat cactcatttc cgagattttc tcaccggtaa
    25381 ccaattcatt gatctctaga cctacttgac ggcacactga acgtgtaact aattcgttgt
    25441 ccccagtcag aacttttaca gctacaccat gttctttcag agctttcaac gcttgtttcg
    25501 ttgtttcttt tggtggatca aggaaagcaa gataaccaat caaaaccatg tctgactcat
    25561 ctttgactga aaattctcca acgacgcttg gattggtctt ttgtgcgaca ccaatcactc
    25621 gtaagccatc ttcattaagt gactgtactt ttgcaagtac ggcttctttt cgttcttttg
    25681 ttaatggtgt aacagtaccg ccaagatcaa taaatttaga aatgttcagc atttcttcaa
    25741 tcgcgccttt tgtgatcatt tgtgttttac cagctggatc agcaaccaca acactcatac
    25801 gacgacgttc aaaatcaaaa ggaatttcat ccactttttg ataattgatc ttatctgtat
    25861 ctaaagtttc tgttgcttca tcaatgatag ccacatctaa caaatttttc agccctgttt
    25921 gatagtagct atttagatac gcatggcgca aaacacgatc atcttcattt ccttcacaat
    25981 ccatatgata ttcaagaatg attttatctt gtgtcaatgt ccccgtttta tccgtacata
    26041 acacatcgat tgcaccaaag ttctggatgg cattcaaatt tttgatcact gttccttttt
    26101 tggccatcac acttgctcct ttgaccaaat tcgtcgtaac gatcattgga agcatttcag
    26161 gtgttagacc aactgctaca gatagtccaa aaagaaaagc ttctagccaa tccccttttg
    26221 taagcccatt gatgacgata actgctggag ccatcaatga cataaatttg atcagtagca
    26281 tggatgtttt atggatgcct aattcaaagc ttgattgaat tggtttttca gatagcgttt
    26341 ctgctacatg accaaatagt gtttcatttc caacagaaac aatgattcct tccgcacttc
    26401 cactgatgac attacttccc ataaagacaa gattttcgta acttgtttcg ctttgctgat
    26461 cgtcttcaca tactttcgca tgcttttcta ctggatagct ttcccctgtc aatgctgctt
    26521 gggaaatgaa taaatccttt gcggacaata aacgcatatc cgctggtatc atatcccctg
    26581 ctgaaagccg aacaagatct ccacagacga tttcttccat aggaatctct gtgtactttc
    26641 cattacgttt tacagctgcc gttactttca caagtgattt cagtttttca gccgcttgat
    26701 tggaacgtac ggactgaatc aacgtcattg tcccgctgac gataaccata gtgaagataa
    26761 tggctacacc gaaaagatct ttatctccag gagctgctaa tacatattcc gtgatgaatg
    26821 aaataattcc taatgcgatc aacactaatg taaatggcgt gatgtatgct ttcaatattt
    26881 ctacaatgaa tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa ttttctcttg
    26941 aacgttcgat ttgttcttct gtcaatccat ttgctgatgc atggaatagt tcaaataatt
    27001 cttgtttcgt ttttccagca aaagctgcat agattttttg tgtatctttc tttttttcca
    27061 tctcaatctc tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg tctttagcta
    27121 ttcatcggat cgatagagaa aaaactgaaa ggggctgaga acagatagat aatagccctg
    27181 gcctttcctc tagatccttg tttttcgaat gactgtcatc catttgttct catctccttt
    27241 actaagtaat tttaattcat gacttccaat catttttcga atagttattt ttccaattag
    27301 accacctcct ttttattttt caccgatggg gatttgcggc aacaaaaaaa cctctcgcca
    27361 aatactcagc gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat ctcatcgttg
    27421 agttttgaca ctgtacaacg taaaaagcag atacctgctt ttagctacct tatgaaaagc
    27481 cttatctcga caattcctgt tctacccatt ggcgtctctc gacgtttttg ggcagtagcc
    27541 tatgtttgta taggagcctc acctaacaaa tcttattcgg ttttccttcg ttaatataac
    27601 aactaaaaaa gaggatgtca aatcaatttt ccatgttttt catacttttt ttaaaataca
    27661 cacgttatat tcctgtctca cgcggtttta tttgtctcat tttccacctc ccgctataat
    27721 agaaatatag taggaaacga ggtgctgtta tggaaagaac acttgttatt atcaagccag
    27781 acggcgtgag acgccattta gtcggaagca tcatccaacg ttttgaagca aagggactgg
    27841 ccattgctga aatgaagttt gaaactatga ctcctgaatt agcaaaagaa cattatcagc
    27901 acttgactga gcgcagtttt tttgatgaac tgatcgatta catgacttcc ggtcctgtcg
    27961 tttatcttgt gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg gttggagcaa
    28021 caaaagctgc cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg ccggggactg
    28081 aaaacatcat tcacgcttct gattcccgtg atgctgcggt aaaagagatc gcccgctttt
    28141 tcccgccatt cgatataaac aaaccgtctg aaaaagcggc gaagtgatac aaattctaaa
    28201 ctgctggtct agttgctggg ttcagcaatg gccagcagtt ttttctctaa ggacctccgt
    28261 tgattctttc ctaacatata ttcgagcact gtgaagaatt cttggtagtc tttcgtcttt
    28321 tcttccccta aagcagcaga aatcaatctt tgataataat ctgccatcaa tgtttcatag
    28381 atcactgtac ttctttcttt atgtgataag ctccgtttca tatcttcatt cacactacgt
    28441 gcctttttca accgaccatt ttctatataa aacgaagtca ggttcattaa aaaagtaaag
    28501 agatactgct catgattgtt caactgtgct aatcgtcgca ttcttttttt gattttcaaa
    28561 taattgttct caatgaaccg atcactgaat atccaaggaa aatcagctag aatagatagt
    28621 tcaaattgac catagtctgg tatctgcatc aagtattctt caatgattcg gcattcataa
    28681 cgtaaaaatt caaattctgt ctcagatact tctgtttttt cgaccatgtg aagattcaaa
    28741 accaaataat acagatcttc tgttttctta aaatgcctac ggttattgct gatctcttta
    28801 taaggatcaa agctgtcttc ttgtacccga tctagcattc gtcttttttt ctttaacttt
    28861 attggttcat ccagctggtt tcgcatccat tcatattctg caagtgtcat gttcattcgc
    28921 tctaaaaatg atctcaatgt tacaaatgac atttttttca ctgaatttgt ctcaatcccg
    28981 actagagaag tcttaaaaca gattccttcc gctaattggg actgtgtcaa cttcttgttg
    29041 actcgaagtt tcttgatca
//
LOCUS       DAZAID010000026        28760 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000026 DAZAID010000000
VERSION     DAZAID010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.026"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..815)
                     /locus_tag="ACG9BE_001095"
     CDS             complement(<1..815)
                     /locus_tag="ACG9BE_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031857.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRW"
     gene            complement(972..1631)
                     /locus_tag="ACG9BE_001096"
     CDS             complement(972..1631)
                     /locus_tag="ACG9BE_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFN3031858.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(1634..2659)
                     /gene="sppA"
                     /locus_tag="ACG9BE_001097"
     CDS             complement(1634..2659)
                     /gene="sppA"
                     /locus_tag="ACG9BE_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFN3031859.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(2672..2962)
                     /locus_tag="ACG9BE_001098"
     CDS             complement(2672..2962)
                     /locus_tag="ACG9BE_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031860.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            3372..5177
                     /gene="glmS"
                     /locus_tag="ACG9BE_001099"
     CDS             3372..5177
                     /gene="glmS"
                     /locus_tag="ACG9BE_001099"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFN3031861.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(5249..6604)
                     /gene="glmM"
                     /locus_tag="ACG9BE_001100"
     CDS             complement(5249..6604)
                     /gene="glmM"
                     /locus_tag="ACG9BE_001100"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFN3031862.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(6628..7797)
                     /locus_tag="ACG9BE_001101"
     CDS             complement(6628..7797)
                     /locus_tag="ACG9BE_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFN3031863.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(7794..8681)
                     /gene="cdaA"
                     /locus_tag="ACG9BE_001102"
     CDS             complement(7794..8681)
                     /gene="cdaA"
                     /locus_tag="ACG9BE_001102"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFN3031864.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(8870..12550)
                     /gene="nifJ"
                     /locus_tag="ACG9BE_001103"
     CDS             complement(8870..12550)
                     /gene="nifJ"
                     /locus_tag="ACG9BE_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFN3031865.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            12748..13269
                     /locus_tag="ACG9BE_001104"
     CDS             12748..13269
                     /locus_tag="ACG9BE_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFN3031866.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(13968..14533)
                     /locus_tag="ACG9BE_001105"
                     /pseudo
     CDS             complement(13968..14533)
                     /locus_tag="ACG9BE_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14712..15368)
                     /locus_tag="ACG9BE_001106"
     CDS             complement(14712..15368)
                     /locus_tag="ACG9BE_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031867.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(15936..16871)
                     /gene="whiA"
                     /locus_tag="ACG9BE_001107"
     CDS             complement(15936..16871)
                     /gene="whiA"
                     /locus_tag="ACG9BE_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFN3031868.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(16905..17903)
                     /gene="yvcK"
                     /locus_tag="ACG9BE_001108"
     CDS             complement(16905..17903)
                     /gene="yvcK"
                     /locus_tag="ACG9BE_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFN3031869.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(17900..18784)
                     /gene="rapZ"
                     /locus_tag="ACG9BE_001109"
     CDS             complement(17900..18784)
                     /gene="rapZ"
                     /locus_tag="ACG9BE_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFN3031870.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(18919..19650)
                     /locus_tag="ACG9BE_001110"
     CDS             complement(18919..19650)
                     /locus_tag="ACG9BE_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFN3031871.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(19654..20919)
                     /locus_tag="ACG9BE_001111"
     CDS             complement(19654..20919)
                     /locus_tag="ACG9BE_001111"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFN3031872.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(21182..23998)
                     /gene="uvrA"
                     /locus_tag="ACG9BE_001112"
     CDS             complement(21182..23998)
                     /gene="uvrA"
                     /locus_tag="ACG9BE_001112"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFN3031873.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(24008..26002)
                     /gene="uvrB"
                     /locus_tag="ACG9BE_001113"
     CDS             complement(24008..26002)
                     /gene="uvrB"
                     /locus_tag="ACG9BE_001113"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFN3031874.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            26261..27763
                     /locus_tag="ACG9BE_001114"
     CDS             26261..27763
                     /locus_tag="ACG9BE_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFN3031875.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            27765..28502
                     /locus_tag="ACG9BE_001115"
     CDS             27765..28502
                     /locus_tag="ACG9BE_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN3031876.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
BASE COUNT         8202 a         6288 c         5021 g         9249 t
ORIGIN      
        1 aaccagcgct tcgggtcttc gatgtatgta tcatagacag gataaacaat tgcagattgc
       61 aacgtgaatt cttcatttaa cacggaacta agcagcgcat cttcattacc gtaacgatct
      121 gaaaatacac ataattgctg gtaacttgcc tgctgctgcg gctttggcag atcttttggt
      181 atgactttga ctaacttttc ttcaatcgtc aaatagaaaa caggatggca ttcgttcaat
      241 tgcctaagtg tctccagtaa ttttgccctt gctaaaatat ggacctctgg cgatacaata
      301 agtttactcg ctgtttggat aaactcatga taaggaagct gttcgatctc atcaatagac
      361 gcaagctgac gtgcaggttc caacgaagga agttcctccg atttattgtg gagtgccttg
      421 atcttctgcc tctccatttg tccggataat tcttctgcaa attggatttt ttcaatgacc
      481 cgctgctcga aagcttcata aaaatctgta cttgctatca attttctagc atataaaaaa
      541 tcttgtgtac tgattaacaa gtcaaaataa aattcagcaa actcctcatt ttgaagatac
      601 tgtttttcat gcaagagtgc ttgcttcaat gcttcttttt tctcgtccaa ctcgtaaaga
      661 caaaaaacaa gcaggctatt agcctcaaag ctttctttca actcgtaagc tcgttgaaat
      721 aattcttttg cctgctgata ttgctggttt gctagtgctt gttgtgctac tcgcattaaa
      781 ttctcatatt tttctggaaa atcaatcggc ttcacgcagt gtcctcctct tgctcatgtt
      841 cagctcatgt tcaatagtag caaaacaaaa aaagcatgac aattaaaaat aactgaagca
      901 ccctctaaaa gctagatttt agatctgact tttagaaggt gctttcaact gttactgctt
      961 tttattgctc atcagctgtt gacttcctgg ttgaatgctt tgatcagatt gatcgtaaca
     1021 acacttgtat tagtgaaaaa atcgcctaga tgttgctttt tctgattaaa gattgctgga
     1081 agatacccta acatcagcaa aggaaatttt agaatgaacc ggcaagcacc ttctctaact
     1141 aaaacggttg accagctaag ttcttcttca tcaaagctga tgactcgaat accaaagatc
     1201 attttcccaa tcgtttgacc atgattgaat ttcgtcaata aaataaaata cgctagataa
     1261 ataagtaagg ctgtgaatcc gtagatactc agataagaat ctgaaaaatt aagccaacct
     1321 agatttttgg caataccaag tgttaaacgc gtaatagctc cgatacacaa aagatcgacc
     1381 aagaaagcaa acattcggat aaaaaaacct gcatagaaat acttaggaaa atcatttgct
     1441 tttggcggtt catttcttgt gtccgtatac tttttccatt tacgttgttt ctttttgatt
     1501 tcttcgggcg tcaaaggctc tctttcaaaa gagctcagta ccttttggga aaagttggag
     1561 tcaggtccta cagcaggctg ctgttgctta tctttttctt gttccttatt ttcatttgaa
     1621 agctctgaca tgcttactca cctccgtaat aatacattgc ttttggtgat tccgctgtcc
     1681 ctagattctc aagaacggat aagatctgac tagtctcaga aggcttcaat ccctggaatt
     1741 ctgctagttt acttcctaac catgtactag caaacccaga tggcgtggaa gaatattcta
     1801 ctagttccgc atcttcaagc tgttgttcct ttcgcatagc ttttaaagca tcttcaggaa
     1861 atcccaattc atcgactaaa cctacttctt ttgcttggac accatcataa atccttccat
     1921 cagcaatttt tttgacttct tcttcagatt tattccgtcc ctcgctgacg attgctacaa
     1981 aacgactata tgcgctgtcg atatatgctt gaagcaccgc ttcttcttct tttgtctctg
     2041 gtcgcgttcc tgaacccata tctttcaacg ctccgctttt aactgttgta tcttccacac
     2101 ccagtttttc tagaagtcct gaataattca atcctgacat gatcacacca attgagccgg
     2161 tcactgtttc ttctgttgca aagattttat ctgcttgagc agaaatatag tagccaccgc
     2221 tagctgccat gtttttcatg ctgacataca ttgggatatc atgttcttta cgaatcgtat
     2281 ccaatttctt agcgatttcc gcactttcgt agacaccgcc accaggagaa tttacttcta
     2341 aaaagatacc tttgactgtt ggatcttctt caatcgcctt cagttgttcc ataaaatttt
     2401 tatgattgta accttcagaa gcaaataaac ctgaagaccc tcccgcagca atcgttccat
     2461 ctactgataa ttttacgatt ttttgcgtac tgtctccctc ttccaacaca tttgaagcta
     2521 gttcttcatc cccatataac caactgttca atccctcaat ttgatcgttt tcctgaggct
     2581 tagtcaaact agctgaaact aacgaaacca caaataatcc tgcagcaatc agaactgctg
     2641 cccaacgtct cttattcatt ctttctcctt tctaatacaa gttttttatg acgatcacag
     2701 tttctgactg ctcagaagtg tcgaaccgct ctgctttttc ttcccgcgca tacccggaaa
     2761 acgactcttt tgtcaattca tcttctaccg taaactcacg agcttcagca acttccaagg
     2821 catcaggtgc gatttcttgg atgctatccc agccagtatc taaaaatatt ttcgtatcat
     2881 ctgatacacc agctgatgct tcgaatttgg acaattgttt ttttaacatt cccagtgtca
     2941 tgatctcaat ttttgaatcc atttttactc ctcctctatc cgattacttt ctattgtacc
     3001 gaactcctga aaaagatgat agataaaaac ccattatttt ttgacataaa aagagcattt
     3061 gacaaagcat ttgatatata ccaatttatt ttttattctt tttttgttga caagaacatt
     3121 cgcaaaaggt tatgatttta aatgtttcca gataaaaaac aaaagaggag gatagttata
     3181 ccttttagga tagcgccagg tctgtgtaaa cagatgacga ggagagggtt tatcgaaaga
     3241 ttcggcggat ggccctaggg actgcactct acagaaaatc cacaaaaaat atctgcaaag
     3301 ataaaacaaa aggattttcc tgcagctgga agcattcata cgaattcatt atagaaagta
     3361 ggatgttttt tatgtgcgga attgttggaa tgattggatt aaaaaatgta acaccaggat
     3421 tgattgacgg tctagaaaaa ttagaatacc gtggttatga ttcagctggt atctttgtct
     3481 cagatggcac aactgattat ctggtcaaag cacaaggcag aattcaaaat ttgaaaaaca
     3541 aaataaatat tgatactaca ggaaatatag gtattggtca tacacgctgg gcaactcacg
     3601 ggcaaccttc tgaagaaaac gcccatccac atacttccca aagtggacgc ttcgtgctcg
     3661 ttcacaatgg cgtaatcgaa aactttgaag aacttaaaat tgcttattta gcaaatgacc
     3721 actttattgg tgagaccgat actgagatta tcgctcatct aattgaaact ttcgcgaagg
     3781 atactacaac gcaagaggcg tttttaaaag ctttgcgggt catcaaaggt tcttacgctt
     3841 tcgctttgat tgatcggaca gcacctgatg ttatttacgt cgctaaaaat aaaagtccac
     3901 tattaatcgg actcggcgat ggattcaatg tgattgccag cgatgcaatg gctatgcttg
     3961 ctcatacgaa ggaattcgtt gagattgaag atgaagagat ggttaccgtc acatcagaaa
     4021 aagttattat tcaaaatttt gcaggtgaca tcgtagaacg ttcttcattc gaagcacaag
     4081 tagattcttc agatatcgag aaaggtacct atccttttta tatgctgaaa gaaatcgacg
     4141 aacagcctat tatcatgcgt cgtatcgctc aaaaatacgt aacagaagac aatcgtgtct
     4201 ctcttgatca aaagctggtc gatacattgt ctgatagcga tcgaatctat atcgttgctt
     4261 gcggaactag ttaccatgcg gggttagcag gtaaacagac tctggaaaaa ttgacacaga
     4321 tccctgttga ggttcattta gccagtgaat ttggctataa cacacctctt ctttcaaaaa
     4381 aaccattctt tattttcttg agtcaaagtg gcgaaacagc agatagccgc caagttctgg
     4441 taaaaatcaa ccgtctcggc tacccttcac ttacgattac aaatgttgct ggttctactc
     4501 tttcgcggga agcatccttt acactgctat tgcatgctgg acccgaaatc gcagtagcat
     4561 caacgaaagc ttatactgca caaatagctg tactaacttt attagccaaa gctatcggtg
     4621 ataaaaaaga atttgaaact tctcttgtat tcgatatcac tcatgaattg agtttagttg
     4681 ctaatgcgat ggaatcggtc atcgcccaaa aagattattt agaagagttg gcagaagtat
     4741 atctcagcga tacaagaaat gccttttaca ttggacgtgg cgctgactac aacgtttctc
     4801 ttgaagctgc tttgaaattg aaagaaatca gttatatcca ggcagaaggt tttgccgctg
     4861 gcgaattgaa acatggaacg attgctttga tcgaagaagg aacaccagtg attggaatca
     4921 tcagtgaaga agtgactggt gcgcatactc gaggaaacct gaaagaagtc gaaagccgtg
     4981 gcgctaaaac actagttatt gtctcagagg gattagaaaa ggaaagtgac cagctggtgc
     5041 taccaactgt ccacccttat ctaactactc taaccacagt cgtaccgact caattacttg
     5101 catattacgc aacgctatta cggggattcg acgttgacaa accacgtaat ttagcgaaat
     5161 cagtgacagt agaataataa aagaacaagg accagtgtca gacatctgac actggtcctt
     5221 gttcttttat tttttatgaa aatatctctt aatccagtcc gatttcttct ttgactactg
     5281 ccgcgatttt atcgacatag taattaactt tctcatcagt aggcgcttct gccatcactc
     5341 gaagtaatgg ttctgtaccg gaaggacgaa ctaggattct tccttctgat cccatttcag
     5401 cttctgcctc ttcaatcact tttttgattg ccggaacatc catcgcaccg tttttatcac
     5461 ttactcggat attcactaat ttttgcggat agattgttac ctctgctgca agctctgata
     5521 atttcttacc tgtctgtttc atgatattca gtaactgaat tcctgaaagc atcccatctc
     5581 cagttgtgtt aaaatctaag aaaatcatgt gcccagactg ctcgccgcca aagttataat
     5641 cgtttttgcg catttcttct acaacgtaac gatcaccaac ttgtgtaacg acatctttca
     5701 aaccgattcc ttccaccgct ttgtggaatc ctaagttact catgacagtc gtaacgatcg
     5761 tatctttttt caatcgattt ttttctgcca agtatctagc acagataaac atgattttat
     5821 caccgtcgac aatgtttcca agttcatcca ccgcaataat acgatcgcca tcgccatcga
     5881 aagctagtcc agcatctgct cctttttcaa cgaccatctc agctaaacgt tctggatggg
     5941 tagagcctac gccatcattg atatttaacc cgtctggatt tgtgcccatg gtataaaaat
     6001 ctgtttcaag atcggcaaac aaacgattaa cacttgttga agttgctccg tttgctgcat
     6061 cgacacaaat ggtcaaaccg gaaaggtcac cgttgatcgt ttgtacaagg aattgagaat
     6121 atttcaataa gccttctgga aattcttcta cagttcccaa tccttcagct gatggacgag
     6181 gcaattcatc cacttcagca tctaataatg cttcaatttc tagctcttgg tcatctacta
     6241 gtttaaaacc atctgcaccg aaaaatttga ttccattatc ttgtgctgga ttatgagaag
     6301 cagaaatcat gacccctgcg cttgcttttt gcaaacgtgt cagataagca acacctggtg
     6361 tagagatcac acctagctgg aacacttcga tacccaccga taaaagtcct gaaatcagcg
     6421 cttcttctaa catttgacct gagattcttg tatcccgccc tactaacacg cgtgggcgtt
     6481 ttccttcttc ttcatgctgg cttaggacat aacctccgca ccgtcccagc ttaaatgcaa
     6541 gttctggcgt aagttccttg ttggcttcac cgcgtacgcc gtctgttcca aaatatttac
     6601 ccatagttgt aaaattactc ctttaactta attttctgag gaatttgttc tgctcatttg
     6661 tgttgattcg cttgtttctg atgtttcttc tgtacttgtg gtcgagctgc ttgtcccaga
     6721 ttccgaactg gaagaaatgg ttgttgtcga tggttccgtt cctgaatcag cagatgaaat
     6781 acttctactc gtacttgtat tttcttgtgt ccgactgctc gtcgaagcag agctactgct
     6841 tgacgtttgc aacacgggtg taatctccac actaactgat tcgggcttga tacttaaccc
     6901 ctcttctact ggtatttcaa ttttacgagt tgttcgatga ttgatacctg agacattgat
     6961 cggtacagca atactgtcac cgatcgatgc aagcaaatcg ctagagccgc ttacttctgc
     7021 ttccagtttt gaaaaactga aatcatagct ttctaccccg gcagctggtg ttccttgctg
     7081 tatcccataa agtcgtaccg acttgcttgg cttgatcgaa tcagctctca ctgttacttg
     7141 cactggatca gagacgatac ttaatacttc ccctgctgca ttcagcgcct gtattgggac
     7201 ctcactatta atcccatcat ctgtgaccat cgaagcgtcc acatcagcta ctactcgttt
     7261 gatttctgcc aatgttttgt cacctgtagt gatagagact tttttaggac taacggaagt
     7321 atcatcaagt tcatatccag atggtgtgaa cgttgaagga atcaacgttt ccacatcgaa
     7381 atcttttatt acctttttct caaccgttac tgtaatagtc gatggttcga tctttgctgt
     7441 gacagcactg ctcaaatttt gaatttttag cggaacctca tgtgttcctt cgcccagctt
     7501 agtgatatct gctgtgacgc ggaacatccg tgtatcttcg tttgcctctg cattcaactg
     7561 aactcggttt gcactggaaa gtttgacagt cactgcattt tcatatccat ggatatagta
     7621 gcgctttgaa tcataagaaa tcttgattgg caccccagta actgtttgat tatacgcttc
     7681 atttccagaa agcgtatttg gaacggactg gccatttgca ttgaagtaca gcgcaatact
     7741 gaacagcaat gcaataaaac tgtaaagaat gcttgtccgt ttctcttttg agatcatttg
     7801 cgatcccctc ccttagtcat accgtctagg aaagattgaa tcacgttttt cttgtttttt
     7861 gttttctctt cagcaatcag ttctctacgc aaaattgcca aatattcttg ctgggataag
     7921 ccagccatca gttcattatc taatgtaatg ctgacatccc cagtttcttc agagacaacg
     7981 atggtaatcg cgtcgctgac ttcgcttata ccgacagctg cccggtgtcg agtcccgaat
     8041 tcttttggaa tcaacatact ttctgataga ggaagatagg cgcttgctac tgcgatcttt
     8101 ccttctttta caataactgc accgtcatgc agcggtgtat taggaataaa gatattgatc
     8161 agcagctctc cagtgatatc tgcatctaat gcaattcctg tctcaatata ctcatctaat
     8221 ccagtatgcc gttcgatagt aattagcgca ccgatttttc gcttggacat atattggatg
     8281 gctttgtcaa aagacaagat catcttctca tcttcctgct gttcgctttt tgattgtttg
     8341 aaaagcgaac tgcgtccaag atgttccagt cctctccgga tttccggctg aaaaatcacg
     8401 atagcagcta tcaccccgta cgtaatgact tggttcatca gccaagacaa ggtgtgcaga
     8461 ccaatgattt ccgctacgat tcggataaca ataaaaatag ctacaccttt cagaagctga
     8521 atcgcttttg tgcctcgcag cagttggatc agtttgtata caagatacca gacaaccaga
     8581 atatcaagta tgttcaccaa aaaattcaga gagaacaagt caccggaaaa catttgctgc
     8641 caatagtcca aattaaaaag ttccccgatt tgaaatgaca taaaaagcct ctcttctctt
     8701 ttgagatttc ctctctagta tatcatatgt tttaatttta gaggctgatc tgctcgtatt
     8761 tttcttgaga aaaacgcata attttcagga aaattgcaag tatgcgtttt atttttctct
     8821 gcaataaata tttttaaact tcagagaatt attcattata ttttttctgt tatttttcct
     8881 ttttttcttt tgtagcttgt tccatttttt tacgtcgttc tgctctggca gctcttcttt
     8941 cttctgcttc aaggtccgtc gttttttctt ttctagtctt aacgggtaca tcgatagatt
     9001 cctctggttc aagtcgtttc ttgatttttt ctaattctcc cgtcagtgca gcatagttgt
     9061 aaaaacgtgt tttagcgtct tggatagtct tttcaaataa agcttttgca agatttggat
     9121 ttgcgttctc tagcgaggaa aagcgaactt gtttcctcat gaaatcaggc atagcagaga
     9181 aatcaggttt tttgtagtcc aaagtcattg ggttcttgtt tttttcgacc attgcagggt
     9241 tatatcgata taatgaccaa tagccagaag aaacagcttc ttttgcttct ttaagcgatt
     9301 ctttcattcc accaagcaaa ccatgtgtga tacaaggcgt ataggcaatg attaaagaag
     9361 gtccaggata cttttctgct tcttcaaaag cctttatcgt ctgcatttga ttggcacctg
     9421 aagcaatttg cgccacataa acatttccat atgtcatcgc catcattcct agatctttct
     9481 ttgaagcata ctttccacta gccgaaaatt tagcgattgc cgaagctggt gttgcttttg
     9541 aggtttgacc gccagtattg gaatagactt cgttatccaa aacaagaata ttcacgtctg
     9601 ctccgctggc aagtacatga tcgatcccgc caaaaccaat gtcgtaagcc catccgtctc
     9661 caccaatcat ccattggctg ttttttacga atagatcctt tctcttgtat atatcctcca
     9721 gttcagggac aattgatcgt tcgctttcga tcgcagcttg cagttttgct gctctttgct
     9781 gcgttccttc gctctcaaac atgtgtacca accagtcttc catcaattct cgtagttctt
     9841 ctgatacttt tggcaatact tctttgattg aacttgccaa agcctcgcga cgagtctgtg
     9901 ccgctagaag cataccataa ccaaattcag cattatcttc caataaagaa tttgaccatg
     9961 caggcccttg accacattca ttggtcgtat aaggagaagc aggagaagca ccgccccaaa
    10021 tagaagaaca gcctgtcgca ttcgcgatca tcattcgatc accgaacatt tgtgtcaata
    10081 attttacata aggtgtttcg ccacagcctg agcaagcgcc cgagaattca agaagcggtt
    10141 gattgaattg tgtagacaag acagtattct ttcctttgac tggatttgct ttttgtctta
    10201 atgtcattgc aaaagcccaa ttcacagctt gttctttctg ttcttcataa ggcttcatca
    10261 ctaaagcttt ttcttttgcg ggacaagcct ctacacacag accacaacct gtacagtctt
    10321 ccactgaaac ttggatacgg tattttacgc cgtctgcgcc tcgcatttct ctcacgataa
    10381 atccttctgg cgcttcttcc atttcctctt catctgctaa aaatggacgg atggcagcat
    10441 gaggacaaac aaaggcacat tcattacaca tcgtacagcg atcactaatc cactcaggta
    10501 cttctaaagc gacgccccgc ttttccatag aagccgttcc taaaggcatt tcgccagttg
    10561 tcattccatt tgctacaagt gtaccaacag agagttgatt tccttcctgt gcattcactg
    10621 gttcaagtat ttcttgtata taaccagtgt tccttttatt gattttttca ggcatctcca
    10681 acgttctcca atggtctggg atcgcgactt gatgaagcag ttcggtcgtc cgatcgattg
    10741 cttgtatgtt tttttctacg atagccattg atttatgtcc atagcttttc agggcttctt
    10801 cttttagcag cggaagcacc tcatcaaaag gaatgatgtc agctaattta aaaaaagccg
    10861 tttccattgc cgtattgatc cggcgtccca aaccgacttc ttgggccaat ctcatggcat
    10921 tgatcgtata gaatctaatg ttattttcag ccaagtaacg ttttaatttt aagggcagat
    10981 gcgtttctaa ttgttcgtcg ctccaaagag tattcaataa aaaaatgcca cctggcttca
    11041 atcctttgac caagtcatag ctatgcaggt aagctggtgt gtgacaggca acaaggtccg
    11101 catgctcgac aagatacgct gaacgtatcg gcgtatctcc aaaacgaaga tgagagacag
    11161 tcagaccgcc agattttttc gaatcataat aaaaatagcc ttgcgcatat ttatcggtgt
    11221 gatctccaat aatcttgata gctgatttat ttgctccaac tgttccgtca gatccaaatc
    11281 cccaaaactt ggcctggaat gtttgcggtt ctgtcaaatc cagactttcc tttggttcca
    11341 aagattgata agtcacatca tccacgatcc ctatggtaaa ccgttttttg cgtatgcttg
    11401 ggtctttttt caattcatca aaaactgctg agatttggtc gggagtcaca tctttagacc
    11461 ccaatccgta gcgaccacca ataacagccg ggcgaagttc agaatcatat aacgcacttt
    11521 gaacgtctaa taagagaggt tctccacctg cccctggttc tttacttcga tcaagaacag
    11581 caattgtttt tactgtatga ggcaactttt gcacgaatgt ttccactgga aaaggacgat
    11641 aaagatggat attcaaaaaa cctgttttcc gtccgtttgc attcaagtaa tcaatcgttt
    11701 gttcaatcgt ctgtgcaact gatcccatag aaacaatgac ttcttccgca tcgtctgctc
    11761 cgtaataagt caccaagtca taagaagttc ctcgtaatcg gttgatctct ttcatatatt
    11821 tttgaacgat ttcaggtaat ttatcataat aggggttgat cgtttcccgt tgttgaaaat
    11881 ggatgtctgg attctggttc gtaccactaa cagtcggatg attgggattc atccctcgtt
    11941 tacgaaatga agctaatttc tcctgattga tcagaggagc taattcttca tattccaaaa
    12001 cttcgatttt ttgaatctcg tgactcgtac ggaatccatc aaagaaattc aaaaaaggta
    12061 cttctccttc aatagaagca agatgggcaa caggtgctaa atccatcact tcttgtacac
    12121 tgctttcagc taacatagca aatcctgttt gtcttgcagc catcacgtct ccgtgatctc
    12181 cgaaaatatt caacgcattt gtcgtcactg cccgactagc aacatggaaa acacttggca
    12241 gtagttcccc agcaatcttg tacatattcg gtatcatcaa aagcagccct tgagaagcgg
    12301 tatatgtagt tgtcagtgca ccagttttca atgatccatg gaccacacca gctgctcctg
    12361 cttcggattg catttctact acttttactg gttgaccgaa tacattttct tttcctgctg
    12421 ctgaccattg atctactaat tcagccatgg tcgaactggg tgttattgga tatattgcag
    12481 caatctcggt aaatgcataa gaaatatatg cggctgccgt attgccatcc attgttttca
    12541 tttttcgcat gattcatcat ccttgttcat attctctctt attatctgtt ccattataac
    12601 gtaaaacatt ccattttttc ttttatttat aaaaatattt cacatatgtc tatttgaatt
    12661 ggtcatctaa tttttagaca aacagtctaa atcgttgcct ttttttgtca gacatgtata
    12721 aactataagc gaaagaggtg ttttaacatg gatatatcag tgattgatgc aacgaaaaca
    12781 aatacacaaa aaggaatcct ttatggttca tcgaatgctc ctaaaaaaat ggtcgaattc
    12841 atcaacctat cttgtccgta ttgccgacaa tggtttgaag aatcttatga gctattagaa
    12901 gaagcagtcc aaagcgggca attgcagcga gtgatcaaat tgttcgataa agaaaaagaa
    12961 agcttgcagc gaggaaacgt gatgcaccgg tatctcacaa tcagcgatgg tcaaaaagcg
    13021 atcaaagaga tcaaacaaat attcgatact caagacgagt ggaaacatct ttctctccaa
    13081 gaagtagcgg attttgcagt agataaacta aagctgacag aacaaaaaga cgaacagctg
    13141 agccaagctg tcatcaacga agctgagcaa gcacatattc gatttgttcc gactgtcatc
    13201 ttgggtaaag aaatctttga tgaatctatt tcaatagagg aattaaaaga attgatccaa
    13261 acaaaataaa aaaataagga gcgtaggaca aaagtaaaga acacttttgt ctcacgctct
    13321 aaacacctat aaacggcgga agcagaagca actccttctt atttcttgga gttaaacgct
    13381 tctgtcccag cctccttatt ccgaataaat ggtgtccaaa agcttgcatc tgccagatta
    13441 tctcatttct tatgacaagc tgagcaaatt atctccacca aataatttat tcatattttt
    13501 gacttgcgca tactaactaa ttcttaaatt ttcatacaaa atttctcaaa ttcccttaac
    13561 gagtaatatt tatgtacggt gattcacact agtatcccaa tcacttttta agttatcccg
    13621 atttcaattc tacttcaaaa atagttgctt ttatgtatgc tgttagttga ttagatggtc
    13681 gtttgttgag ctaggtcgtt tattttaaac aagttgtttc tttagcttat acctatctcg
    13741 tctaccaaac gcaagatggc aaacacaaat gcatacgcat ctgtatttgc cactttgatt
    13801 cgtataaagc taacaactcc taactcctct catttaatag ttgctctctt ttttattcag
    13861 atacattttt gctgatcaca gcctagtatt tcaatggttt tgaacactaa accaaattgt
    13921 tccattacaa cattcactgg ctgatgaaat ggattaatag atttcgatta ttcaaagcga
    13981 ttttctattg gtggattgtg ttcccaacta acaagacgga ttccaaagtc attatataac
    14041 tcttcaagag ttaacccttt cctgttccca atagatgtat ctctcccacg ctcgatataa
    14101 aatatggagt ttcgaatgcc aagacctggc aaatctttcc ttgttacagt tacactgcta
    14161 ctaacttccc catataaaat ccagctcctc caaaatgata atatattttt cgaccatcat
    14221 ctaactcatg taacaataat tgttgtgccc tcttgtcact cgccaaatgc aaacgccacg
    14281 tgcgatacca tgccatgcta cctgcatctg cagaaaatcc atcaaataat gcaacaattg
    14341 tatcttctat cacaatcctt tccccatcta cttcatattc taatctaaaa tcaaactccc
    14401 ctcgggtaat cgcaggtctt ggtggcttag gacccaatat tattctactt aaaaccgcat
    14461 tcgttcccag atgaaacaca gctataacaa caataatgat taatattttg tttctatttg
    14521 ataaatttct cattattcgc aaatcacctc tacattctca catactagtt cgtaatactc
    14581 ctttaatatc aaaatatcta ctgatcgtaa taatattcgc tatgaattcc tactactcgt
    14641 ataagaaagg ccaaatcatc agcttttact tgctgatgat ttggcttgtt tctatttatc
    14701 gtaattgttt tctaatcacc aaaattgtta acaatcggtt cagatatttc ccaattcact
    14761 agccttatat tatattgatt taacaattct tcttcattta ttgtattttg atcaagcgga
    14821 ccaaattgtt ctaataattc tttgtcatgt aaaatatccc ttctagtaat gcctctttcc
    14881 ctttcaatga aagaagcact aggaaaagta tgattataac cattcaacct atctcccatg
    14941 taataattag caggtcctgg aaaataaaat atttgcttat tatcactaac ttctaacagc
    15001 aacactaaat cttctccgct ggctaaacga cttgtccatc tacgatatct ccctatgcct
    15061 tcgttcattc caaatccatc aaattcaact attaacgtgt cttctatcac atgtaattct
    15121 tcattcaatt catactcaag tctaaaagga aattctccat atgtaatctc tggcaatggt
    15181 ggtgctggag ttctccaaat aaatacaata actgtgaata gaaaaaatcc aacgatcgat
    15241 ataaatgtta tgagacctgc tcgaagtaaa acccgatata ataaaaatgc tgtatttcgc
    15301 tttgctatat ctagaaaaat aaaaagtact gcaatcataa tgatagtaaa agttgtaaaa
    15361 aaacgcattt ttgccctccc tttataaaca tcgattctca aattctaaac caaatcttgg
    15421 tagttcaagt tcaggtataa cgtattccaa ccatacatct caattactat ttatgaactt
    15481 atgattatca ctgaataaat cactccatcc ccttgttaaa acttctacat taattacacc
    15541 tggttgtttt cgactagcta atccaatgaa cttatcaacc atccttatcc accaaccacg
    15601 agaatcttga ccagaagtaa gaagagcagt tctcaaggat gatcgagcag ttccttccag
    15661 ttttttaatt aatcaggcca gttatagata aatacttgat cttatctata tcaggacttc
    15721 tatattaagc aaaaatttat gtacaaaact aaaatcttca ttcagttgtc tccccgcttt
    15781 ttatacgatt gtattaaaaa caatagaagc ctttccaaat tatgttactc cagtaaagat
    15841 ggaattatag tttgacggaa gaaaagcaag tataactttt aaaaaaaact gatacattcg
    15901 catctcctgc ttcatgtatc agttttttct ttggattaaa ctgcttcttc acgcagtttt
    15961 tccgcaaatt catttatttt gcgaattcgg tgattaattc ccgattttga tatcgcacct
    16021 gagggaatca tttcccctaa ttctttcaag ctgacctctg gatgttgaag ccgaagttct
    16081 gcgatttcct gaagtttttc aggaagggca tgtagaccga cctttgcttc gatcaattcg
    16141 atgttttcga tttgtttaga agccgcatca atcgtcttgt tcagatttgc tgtctcacaa
    16201 ttgactagac gattgaccga atttctcata tcccgtacga ttcgaacatc ttcaaatcgg
    16261 agcattgagt tggttgcacc aatcaatgtt aagaaatcag caattttttc tgctcctttt
    16321 aaataagaaa tatagccatt gcgccgttca agcgttcgag catgtagacc gtaataattt
    16381 aacatatcgc aaatgtcttg gttatgctgt tcataaatag aaaatatttc caaatgataa
    16441 cggctcgttt ccggattatt gactgaacct gaggccataa aagcaccacg taagtaagaa
    16501 cgcatttttt gcgaatttcc cataatctcg tccgaaacgt gtccattgaa catcatacca
    16561 tccatgatat caagatccat caacacatct tttgtgcctt gcttcaaccg gacgatatag
    16621 acattgttct tttttaattt cattttttta cgaacgagta gctcacttcg tacctgataa
    16681 tgatctttca aaagagaata gatacgtctg gcaattgcag cattttctgt ttggacgttc
    16741 aaaataaact gctggttcac aatacctaaa gagccgttca ttcgcagcaa agcagctaat
    16801 tccgctttcg catgttctcg atgaacttcc aaaccagtta gttctttctt cacatctgca
    16861 gcaaatgaca tcctattctc ctcctttctc caacaataaa ttgcttaata ttttgaaccg
    16921 aaaactaaac ggaacaattc ttctactact ttgtctccat cgtggaagac accaccatct
    16981 ttcaattcca aaaagtcggt ggaaatcact cgacatcctt catttctcaa tccgttgaag
    17041 tcgtgtgcga cttgtactag atactcatca tagatctctg ggtccatata atcttcaggc
    17101 actttttccg tattgaccaa tactgtatcg ataaaaggct gattcaaatg cttgtgtaaa
    17161 acacgtatat gatcggcatc tgtgaaatgt tccgtttccc ctttttgggt catgatatta
    17221 cagatataga cggtttcagc agttgtcttg agcattgctt cgccgatttc tggtatcact
    17281 aaatttggaa ggatactggt gaataaactt ccgggtccca aaacgaccat gtcggcgtcc
    17341 ataattgctg aaacgacttt tcgagccgct ttggcttcac ttttatcatt tgtgttgcag
    17401 acatacacac ggtcaatggt tttacggtcg atcgcgattt tagattctcc tactgcagtc
    17461 gttccgtctt taaagacagc atgcaaggtc aaaggagttt ctgaagaagg ataaacatgt
    17521 ccatctacat gcatcatttt tgctaataat tgaatcgcct cataagtgct tccccgcatc
    17581 tcggaaactg cggcaatgat cagattaccg atcgtatgat tggctaaaaa ctgatcttcc
    17641 tttttgaaac ggtattggaa aatatcttca tagaattgtg gcatatccga taacgccact
    17701 aagacatttc tcaagtcacc aggaggagcc atagcgatcg attctctgat tgcaccgctg
    17761 cttcccccat catctgcaac agtcactact gctgtgatat ctgctccttg atttcgcaaa
    17821 cttttcagga taactggcag accagtgcca ccgccgacca ctacgatctt tggttttctt
    17881 atccggtagg ttttcatttt cacgaccggt tcaccgtctc tttcctttta tccttgtccc
    17941 gatgtgtcac attgacttta tagtttttcc ctaaagcatt tgccaaacgt tcagtcaatg
    18001 ccacagaacg gtgttgaccg cctgtacagc cgattgcaac tgtcaagctg ctttttcctt
    18061 ctttaatgta tccaggcaat acagtttcca gaagatccaa aaatttatgg tagaatgatt
    18121 gcgtttcatc aaaggacatc acataatcat agactggctg atcttttccg gtcaatggtc
    18181 ttagttccgg gatataatga gggttgggca aaaaacgcac atccatcaca atatccgaat
    18241 cgatcggcaa cccgtattta aaaccaaatg atacaaattc gatccgaaat ccttggtctc
    18301 cctttgattt gaatgactca ttgatttttt cacgtaattg tcgtggtgtc aatgtcgtcg
    18361 tatcaatgat cacggaagct tgggctttca agtcgtctag tatcgcccgt tctttacgta
    18421 tcccttcagt gatcaaaccg tccatcgcca ttggatgtgc tcgtcttgtt tctttgtagc
    18481 gagccactag ttcttcgtct gaagcatcta aaaataagat agaagtgtcg atgaagttcg
    18541 tattttcgat ttcgatcagc atgttttgga tttcttcaaa gaaggaacga gaacgtaaat
    18601 ccactcctaa agccattttt gtgactttgc ctgattcttt gattaattcc cagaattttg
    18661 gaatcaatcg aggcggcata ttatcgatac aaaaatagcc catatcttca aaactttgga
    18721 tagctactgt tttccctgca ccgctcatac ctgttatgat gactaattgc aaattatctg
    18781 tcataattac gaactcctct cacattgtgc atagtaaaat tatatcattc cgggcgtatc
    18841 attaccagtt ttctttttct tttacaaaaa gatttggacc aaatatcttt taggatattt
    18901 gccccaaatc caattttttt acttttccga tgctgtatcc aatgccttgc ttctttcagc
    18961 tagcgctcgt tcaatcgtcc gatctgctaa aatagattgg gcatcgatca ctggataatg
    19021 gttatcttca gcaaattcat gcatcaatga caattctgtg caacctaaga taactttttc
    19081 acagtttagg cgctctaccg cttcttctaa gatttcgtaa tatagctctt gattcaaata
    19141 atccgattcc ttgatctcat gatagatcag ataattgatt ttttcttgaa gaacagtatc
    19201 aggaatcact gtttcgaatc caagattttt gacttcgcgt tcatagattc cagctttcat
    19261 acttccttct gttcctagaa tcgctactct ccctgtcgta tgctgacgga caagttcatt
    19321 tgctgcttct cttggcatat gaaggatagg aatatccgtt gccgcttgta attcttcaaa
    19381 aaagtagtgt gcggtgttac aagtcaacac aatgaagttc ggctgcaata aattttgttt
    19441 ctcaatatca tccagcagaa acggcattgg attttcttct gatcgatcta aaatataagc
    19501 tgtacgatca ggaactgttg catgattgaa taagacataa ttcaaatatt cttgatcttt
    19561 tgtagctttc gttcggtggt tgatcaagcg aacaaaactt tctgtcgcca tcgtgcccat
    19621 tccgcctaaa atactgaaaa aattctccat gaactagcct tctttcacat gaaaatactt
    19681 tttgaatctt ggaatatagt tatggaacat atatttcatc aacaaccagc gcttgattga
    19741 ccggtctttt tcataaaaca cagtagtacc gtaacgattt tctttgatca tttgctcagc
    19801 tagttttttg tcttcatttt cccgagcata ttctaaaaag atttttttgg gcacacccat
    19861 ccataatctt gctttatctg attgattcgt tccataggtc gtttcaacaa agggtttgtt
    19921 aaacactcga tcttctgtga cgaaacgagc cagattcaag ccgttcaatg tcacaaagaa
    19981 actgctacga ccttgtctca aattgatttc aaataattta tattctccat ctctaggatc
    20041 atacttcata tcgaaattgg caaagcctgt atattcgatt ttttccaaaa atgccttaat
    20101 ggtttgatag attttttcgt tataatctgg catgatcaca acgtaattac caatcgatgc
    20161 tggtgtagga tcttctaaca gtggatgacc aagacacatc atccgaactt gatgatcttc
    20221 atctacatac gcatttagca cacgcatatt actatcatca cccgggataa aatcttgtac
    20281 aatcatttca cttttataac cggcttcgta tatccggcct aaaattaaat cgaactcttc
    20341 tctagtctct aaaataaaag ctttttttct tccttcaaat tggacagaaa gatactccac
    20401 actgtttgca ggtttcaaag caactgggaa ttcaaaaggc aattcctgtt ccaaatgtcc
    20461 atttactaac atttcttctc tgacgatcaa tgttttagga tacggaagat catattcttc
    20521 acaaacttca taaaagctta ctttattgat cagtctttca aataaagaat aatcaatata
    20581 tggacagata aaatattctg acagctcttg tttatgttgg gagatcaatt ctgcatatcc
    20641 atcaccacaa gcaatcaaaa gatatttttt actcttatca gaatatcttt cttttgccaa
    20701 acgaagcatc gtctcgataa acacagggtc tttatcaaat cctggaataa cttctacatt
    20761 gacaatttta ctgtagcggg taggggctaa ctgatccgaa gcatatgctt ggcagatttt
    20821 gccgtatgct tcattaaaag aacgtgccat accataaacg ttcatgtcgc ttcctaataa
    20881 tatagggata aattcttcat tttcaatact gttcatcatt atctctcact tactcataat
    20941 ctcttgtttt tcttttccaa ctacacaagg aatatcatac cataaagaaa aaagcaatca
    21001 aaggaaactc ctccgattgc tcaattcttt tttgaacgga ttgaacttat aggaagtttt
    21061 tgtctatacc aatcagtgta gaaagaacta ccatgtaatg attctagaac actgatatta
    21121 ataaaacaag caaaaattca tttttttatg tttttttaac tatctcagct atctatcctc
    21181 tttataccat tatttttttc aaatagtgac ctgtataact ttcttttacg ttaatgattt
    21241 cttctggtgt tccagtcgca aggatcgttc ctccaccttc tccaccttct ggtcctaaat
    21301 caatgatata atctgctgtt ttgattacat ctagattgtg ttcaatcact aaaactgtgt
    21361 taccagcatc tactaatctt tgtaatacat gcaacaagcg ggcgatgtca tcgctatgaa
    21421 gtcctgtcgt tggttcatct agtatataga aattctttcc attagagatt ttgtgcaatt
    21481 cactggcaag tttcatccgt tgtgcctcac caccggacaa tgtcgttgct ggttgcccca
    21541 tcgtcacata acctaagcca acatcaacaa tcgtttgcag tttgcgatga atctttggaa
    21601 tgtgcttgaa gaattctaca gcatcttcta ctgtcatttc caaaatatca gcaatgcttt
    21661 ttcctttgta atgcacttct aatgtttcag agttatatcg tttgccatga catacttcac
    21721 aaggaacata gacatcaggc aaaaagtgca tttcgatctt aataattcca tccccgcgac
    21781 aagcttcaca acgaccgcct tttacgttga aactaaaacg ccctttctta taaccccgca
    21841 tttttgcctc gttcgtttga gcaaataacc cacggatatc atcaaataca cttgtataag
    21901 tcgctggatt acttctcggc gtccggccga ttgggctttg atcgatatcg atgatctttt
    21961 cgatactttc gtagccggaa attgtcttga atttacctgg cttagcagaa ttcttattta
    22021 atttttgcgc taacgatttc tttaagatac tattgaccaa tgtactcttt ccagaaccag
    22081 acacacctgt gactgctaca aattctccta atggaaattc aacactaata tttttcagat
    22141 tgttttctga agcaccagtg atcttgattg ctttcccatt acctttacgg cgttcttttg
    22201 gtacagggat tgattttttc cctgaaagat atttccctgt caaagaattt tcatcattag
    22261 ctacttcttc aggagtgcct gcagccacga tttgtccgcc ttgttctccg gccccaggtc
    22321 cgacgtcaat cagataatct gctgcccaca tcgtatcttc atcatgttcg actacaacga
    22381 gggtgttgcc aagatcacgc attttcttta atgagccaat cagtcgatcg ttgtcgcgtt
    22441 gatgcaatcc gatcgatggt tcatctaaaa tatacaaaac tccagaaaga ttcgatccaa
    22501 tttgtgtagc caatctgatt cgctgcgctt ctcccccaga taatgttccg gaagcgcgac
    22561 tcaaagttag atagtctagt cccacatttt ccaagaatga aagtctgtca tttacttctt
    22621 ttaaaatcgg tttggcaatt actttctctt gttcagataa cgttaagttt gagaagaaat
    22681 ctactgcttt ttgaatagac aactcatttg tttgcccgat attcgttcca ttgatttgaa
    22741 cagatagagc ttggggattc aatcgataac ctttacatgt ctgacaagtt aattctgtca
    22801 tataaagacg catttgctca cgcgtgaaat cactgttcgt atcatgataa cgacgctcga
    22861 tattcttcaa taccccttcg aaagtcgttt ctacatcgcg aacgccacca aaatcatttt
    22921 cataatggaa atggaaaaga tcatctgtcc cattcaagat gatatcctga tgctcttccg
    22981 gtaattcttc gaatggtgta tctaaatcga taccgaaact ttcacaagcc tgcgctaaca
    23041 tttgaggata atactgagag ctgattggat tccatggaac gatagcacct tgactaagtg
    23101 tcttcgttct atctggaatc actaaatctt gatcgacttc taatttgaca cctaaaccat
    23161 cacagtctgg acaagctcca aaaggcgcat tgaatgagaa cagtcttggt tctagttcgc
    23221 caacagtaaa tccgcaataa ggacaggcat agtgttcgct aaacaacatt tcttcttcac
    23281 cgatcacgtc aaccagagca tagccgtctg ccaatcgcag agccgcctct aaagaatcaa
    23341 atagacgaga acgaatccct tctttcacaa tgatccggtc gatgacgatc gcgatatcat
    23401 gttttttgtt tttctccagc tcaggcactt cacttaaatc atagatctca ccatcgactc
    23461 gaacgcggac gtatccctct ttttggattc gttcgaatat ttttttatgt tgtccttttt
    23521 tctttaccac gatcggtgcc aacacttgga tctttgtgcg ctcaggcaaa gccattactt
    23581 gatctgccat ttgatctact gattgactgg taatctcgat atgatcgttg ggacaaatcg
    23641 gatgtcctac acgtgcaaac aacaagcgta aatagtcatt gatttccgtc acagtaccta
    23701 ctgtcgaccg aggattttta cttgttgttt tctgatcaat agaaatcgcc ggactcaatc
    23761 cgtcgatgct atctacgtcc ggcttatcca tttgtcctaa aaactgacga gcataagcag
    23821 aaaggctctc tacgtatctc cgctgccctt cagcgtacag cgtatcaaat gctagggaac
    23881 tttttccaga accggacaaa cctgtaacga ccacgaattt atctcgtgga atcgttacat
    23941 ctatattttt caagttatgg gcacgtgcac cgtgaatcgt aattttatcg ttagccatgt
    24001 tgtcctccta ctttgaagct tttaattcta aaatcatatc tcgcacatta gcggctgttt
    24061 caaaatccaa tgctttcgcc gcatttttca tttctttttc taatttcatc aataacgtat
    24121 ctttttcttc tttcgtcatt tcttcatacg agacttgtac cgcttctttt gtatcatctt
    24181 ccgattcttt cgtaatagaa atcaaatctc ggatttcttt aatgatcgtt tttggaacga
    24241 tcccatgttc ttcattgtac ttctgctgaa tcgtacggcg tcttgatgtt tcatccatcg
    24301 ctagacgcat agaatctgtt actttgtctg cgtacatgat tactttacct tcttcattcc
    24361 gagcagctcg tccaatcgtt tggactaagg aacgttcact acgtaaaaag ccttctttat
    24421 ccgcatccaa gatagcaaca agggaaactt caggtacgtc taatccttca cgaagcaaat
    24481 tgattccaac taacacatca aattcaccta atcgaagatc tcgaatgatt tctgttcgtt
    24541 ccagcgtttt gatatcacta tgcaaatatt tgactttgat ccctaattct ttgaaataat
    24601 ctgtgaggtc ttctgccatt tttttcgtca atgtcgttac aaacacccgc tggtcttttt
    24661 cgactcgttc atttatttcg cctactagat catcgatttg tcccatgatt ggacgaactt
    24721 cgatcactgg gtcaagcagc cctgtcggac ggatgatttg ttggataacg gtatctgttt
    24781 gttcatgttc atatggtcct ggcgtcgcag agacataaat gatctgattg acatgttttt
    24841 caaactcttc taaccgaagt ggccgattgt ctaaagcaga tggcaaacgg aaaccgtaat
    24901 ctactagcat ctgttttctc gctcggtcac cattatacat tcctctgact tgaggcatgg
    24961 ttacgtgcga ttcgtccgcc acgatcaaaa aatcttcagg aaagaaatcc aatagcgtat
    25021 aaggcggttc accttcttta cgtccgtcca tatgccgaga ataattttca attcccgacg
    25081 tgtagcccat ttctaacatc atttcaatat cgtaattggt tcgctgttct aaacgttgag
    25141 cttccagaag tttgttttca tttcgcaaga cagttaaacg ctgttctaat tccgctttga
    25201 tattcgctac ggcatgctcc atatgttcat cattcgtcac aaagtgagtc gccgggaaga
    25261 ttgccacgtg ttccgtttct cctaaaactt cacctgtcag tgcattgact tctcggatac
    25321 gttcgacctc atctccaaaa aactccacac gtaaggcacg ttcatctctt gaagccggaa
    25381 aaatctccac gacatcaccg cgcactcgaa aacgaccgcg ctgaaaatct atatcatttc
    25441 tttcgaattg gatactgact aagtcacgaa tcaactgatt gcgatctagt tctgctccct
    25501 gtcgaatcga aactacttgt ttttggtact caaaaggcga acctaatcca aaaatacatg
    25561 aaacggatgc aatgacgatc acatcatttc tttctaataa tgaactagta gccgaatgtc
    25621 gtaatttgtc gatctcatca tttacacttg aatctttttc gatatacgta tcgcttgaag
    25681 gcacataagc ttcaggctga taataatcat aataagaaac aaaatattca acagcattgt
    25741 tagggaaaaa ttccttgaat tccccgtaca gctgaccagc caatgttttg ttatgggcaa
    25801 taattagtgt tggtttattc actttctcaa taagattgga aatcgtatat gttttccctg
    25861 ttccagtcgc acctaataag atttgtgctt ttttcccgcc gactacgcca tccactaatt
    25921 ggttaatcgc ttcaggctga tcaccagccg gttggtattt cgagacaagc tcaaagtgtc
    25981 gggaggtatc tctctcaatc attcttcgtc ctccttttca ttcattcctt ttcattttac
    26041 gaccaataaa aagaaaagga aagcaaaggc attccttact aaaaaattat accacaccga
    26101 acatactttc gccacttttt tatcttatta tgcatgttac gttttataac ataaaatgtt
    26161 agaaactcgc atttttttca ttttttcgag cttacagtct tgtctgattc agcacttttc
    26221 gcctataata ataaaatatt ggaaatttag gaggttgatt atgaaaagaa agcatttatt
    26281 tcactcattt gttttcttaa tgacaggact tgtgacactt gcgtttggac atgttgcaca
    26341 agctgacaac actacaacgg cagagtcaag cacaagcgag acaatgcaaa aaatagagcc
    26401 caaaaaagac gtatacgtaa ttgccagtga ctctgctttt gctccatttg aatttcaaaa
    26461 cgcaaagggc gagtacgaag gcatcgatgt aaaattgatg gaaaacatcg ccaaactgca
    26521 aggatttaac atccgaatgg actaccgtgg tttctctgct gctttgcaag ctttggaatc
    26581 aggacaagca gacggaatga ttgccggaat gactgtaaca gatgaacgaa agaaaagcta
    26641 tgatttttct gattcttact ttgatagcgg gatccaatta gctgtcaaaa aaggagatac
    26701 ttcgatcaag tcttatgaag atttgaaagg aaaaacagtc ggagcaaaag ttggtaccga
    26761 aagtgctgaa tttttaagcg accatgaatc tgaatatggc tattctatca aatattttga
    26821 tgctgctgat caattgtacg acgggttaaa agttggcgca atcgatgcaa tgatggatga
    26881 ttatccggta atcggttatg cgatcaaaca aggacaagaa ttggaaacac cgatcaaaaa
    26941 agaatctggc ggaaaatatg catttgccgt taaaaaagga caaaatcctg agcttcttaa
    27001 tatgttcaat gaaggattga aagaattaaa aagaacaggc gaatacgata agattattaa
    27061 agaatatgta gcggatggtt cagaaacttc cagtgagtct tccactgctg atgaatcaac
    27121 atttcttgga cttatccaaa acaactatca agcattgttg aaaggacttt ggagaacaat
    27181 tctcctaact ttgatctcct ttgctttggc tatagtaatc ggtgtgattt ttggattatt
    27241 cagtgtagca ccaatacgcg ctcttcgcac aattgctagt atttatgtcg atatcatccg
    27301 aggaattcca atgatggttc ttgcgttctt tattttcttt gggttgcctg gtatcgttgg
    27361 atttacgatt cctgacttcc ttgctggtgt tatcaccttg actcttaacg caagtgctta
    27421 tatcgcagag attgttcgag gcgggatcaa cgccgttcct gtcggacaaa tggaagcttc
    27481 cagaagttta ggtctttctt ataatcggac aatgcaaaaa atcattttgc cgcaagcgat
    27541 acgcatcatg atcccatcat tcgtcaatca gttcgttatc tcattaaaag acacaacgat
    27601 catttccgca atcggtgtag tcgaattgct gcaaactggt aagatcattg ttgcacgtac
    27661 aacacaaagt acgtatgttt acttgatcat tgcgatcatg tacttgattt tgatcacagc
    27721 attgacaaaa ttagcaaaag ttttagaaaa gaaggtgaag taagatggca gaaaaaatct
    27781 tagttgagca tttagtcaaa aaatacggcg ataacacagt attaaacgac atcaatgtgt
    27841 ccatcaacga aggagatgtc gtctgtatca ttggcccttc aggttcagga aaaagtacat
    27901 ttttgcgttg cttgaatcgt ttagaagaac cttctgacgg tgaaattatt attgacggcg
    27961 cccacttgat ggataaaagc acagatatca acttggtgag acaacatatc ggaatggtat
    28021 tccagcactt taacctattc cctcatcttt ctattttgga aaacatcatt ttagcaccga
    28081 tggacctgaa gaaagaatct agagaagaag cagagaaaaa agcaatcaaa ttgctgcaga
    28141 cagttggtct agaagataaa aaagatatgt atcctgaaat gctttccggt ggacaaaagc
    28201 agcgtgtggc tattgctcgt gctctagcga tgaaccctga catcatgctt tttgatgagc
    28261 ctacatctgc actcgacccc gaaatggtcg gtgatgtctt gaacgtcatg aaaaagttag
    28321 cagaacaagg tatgacgatg gttatcgtta cgcacgaaat gggcttcgct aaagaagtgg
    28381 ccaatcgtgt catgttcatt gacgacggaa actttttaga agacggaacg cctcaacaaa
    28441 tctttgaaaa tccgcaaaat gaacggacaa aagatttctt gaataaagta ttaaatattt
    28501 aataaaaacc aaaaatgccc ggtcgcttca tgacgaccgg gcatttttag tttcttgttt
    28561 tttcacttaa ctgtaccagc cgcatcgctt gttctcctgc acaaacaagc aatcgctcta
    28621 cctgttccat cgcataagaa agtaccatcg gttcattgac gatatcaagt acatagtcga
    28681 taccaccttc agaaactatg atgattatct tattctaaag ctggcttttt tgttggtaca
    28741 aacatgacaa ctacaaaaaa
//
LOCUS       DAZAID010000027        27796 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000027 DAZAID010000000
VERSION     DAZAID010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.027"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            283..2022
                     /gene="yfcC"
                     /locus_tag="ACG9BE_001116"
     CDS             283..2022
                     /gene="yfcC"
                     /locus_tag="ACG9BE_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFN3031877.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2179..3363
                     /gene="iadA"
                     /locus_tag="ACG9BE_001117"
     CDS             2179..3363
                     /gene="iadA"
                     /locus_tag="ACG9BE_001117"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFN3031878.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3474..3677
                     /locus_tag="ACG9BE_001118"
     CDS             3474..3677
                     /locus_tag="ACG9BE_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFN3031879.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3779..4441)
                     /locus_tag="ACG9BE_001119"
     CDS             complement(3779..4441)
                     /locus_tag="ACG9BE_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3031880.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4454..5956)
                     /locus_tag="ACG9BE_001120"
     CDS             complement(4454..5956)
                     /locus_tag="ACG9BE_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3031881.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6504..8183
                     /locus_tag="ACG9BE_001121"
     CDS             6504..8183
                     /locus_tag="ACG9BE_001121"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN3031882.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8310..8789
                     /locus_tag="ACG9BE_001122"
     CDS             8310..8789
                     /locus_tag="ACG9BE_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFN3031883.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            9008..9688
                     /locus_tag="ACG9BE_001123"
     CDS             9008..9688
                     /locus_tag="ACG9BE_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFN3031884.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9696..11027
                     /locus_tag="ACG9BE_001124"
     CDS             9696..11027
                     /locus_tag="ACG9BE_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFN3031885.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            11042..12004
                     /locus_tag="ACG9BE_001125"
     CDS             11042..12004
                     /locus_tag="ACG9BE_001125"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFN3031886.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12115..12453
                     /locus_tag="ACG9BE_001126"
     CDS             12115..12453
                     /locus_tag="ACG9BE_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFN3031887.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12532..13239
                     /locus_tag="ACG9BE_001127"
     CDS             12532..13239
                     /locus_tag="ACG9BE_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFN3031888.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13342..14823)
                     /locus_tag="ACG9BE_001128"
     CDS             complement(13342..14823)
                     /locus_tag="ACG9BE_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3031889.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            15132..15668
                     /locus_tag="ACG9BE_001129"
     CDS             15132..15668
                     /locus_tag="ACG9BE_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3031890.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15746..17116
                     /locus_tag="ACG9BE_001130"
     CDS             15746..17116
                     /locus_tag="ACG9BE_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFN3031891.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17183..17518
                     /locus_tag="ACG9BE_001131"
     CDS             17183..17518
                     /locus_tag="ACG9BE_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031892.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17599..18456)
                     /locus_tag="ACG9BE_001132"
     CDS             complement(17599..18456)
                     /locus_tag="ACG9BE_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3031893.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18562..19077
                     /gene="tadA"
                     /locus_tag="ACG9BE_001133"
     CDS             18562..19077
                     /gene="tadA"
                     /locus_tag="ACG9BE_001133"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFN3031894.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            19100..19184
                     /gene="ffs"
                     /locus_tag="ACG9BE_001134"
     ncRNA           19100..19184
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG9BE_001134"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19394..20779
                     /locus_tag="ACG9BE_001135"
     CDS             19394..20779
                     /locus_tag="ACG9BE_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFN3031895.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20816..21493
                     /locus_tag="ACG9BE_001136"
     CDS             20816..21493
                     /locus_tag="ACG9BE_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFN3031896.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21501..22265
                     /locus_tag="ACG9BE_001137"
     CDS             21501..22265
                     /locus_tag="ACG9BE_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFN3031897.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22267..22926
                     /locus_tag="ACG9BE_001138"
     CDS             22267..22926
                     /locus_tag="ACG9BE_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFN3031898.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(22956..23465)
                     /locus_tag="ACG9BE_001139"
     CDS             complement(22956..23465)
                     /locus_tag="ACG9BE_001139"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFN3031899.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23577..24575)
                     /locus_tag="ACG9BE_001140"
     CDS             complement(23577..24575)
                     /locus_tag="ACG9BE_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN3031900.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            24776..25360
                     /locus_tag="ACG9BE_001141"
     CDS             24776..25360
                     /locus_tag="ACG9BE_001141"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN3031901.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25437..26612)
                     /locus_tag="ACG9BE_001142"
     CDS             complement(25437..26612)
                     /locus_tag="ACG9BE_001142"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFN3031902.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            26694..27074
                     /locus_tag="ACG9BE_001143"
     CDS             26694..27074
                     /locus_tag="ACG9BE_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFN3031903.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27148..27510
                     /locus_tag="ACG9BE_001144"
     CDS             27148..27510
                     /locus_tag="ACG9BE_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031904.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         9055 a         4685 c         5788 g         8268 t
ORIGIN      
        1 tgaaattcaa tttttcaatg attgtaaaat gaaaaaaaca ggacgagatt ttctctaatc
       61 taaaaataaa aaaagaattt taaggaaaaa cagagaaata aaatacttaa tataaatgga
      121 aagagaaaaa aataatcata cataggagaa aaaaaacaaa atagaaaaaa gtgattttct
      181 aataaattat tcatgtggaa atgtcatttt tactgtataa ttcatttgat tatccatttt
      241 ttattcatca aaattactga aaaaagaaag gaagaaataa atttgaagaa aacaaattct
      301 tcaggaaaac cgaaacgcga cttcactaaa ttatcgacac cccatacgta tgtcattatt
      361 tttggtgtag tcatctttgc atggatactg acttttgtcg ttccagcagg aaagttcagc
      421 acgcaagata ttgaatacaa agatgcaaat ggcgaaacaa gtactcgtac tgttttacgt
      481 caagattcct tccgttatgc ttacgagctg gataagtcgt atgtgtttga tcaattagaa
      541 gaactgcaag atcatccagc agaacgtgaa aaactggatg ttccggaaaa aggattggaa
      601 aaagtcattg ctgacggaga aaaaaatctt acgcaggaaa aactagatga aatctccttg
      661 acagatgatg tgctttacga tcagtacggc gaaaatatct atgatacttc taaaaaattg
      721 cataaaacag ccaaaatatg gggaacagat gattttggcg gctttggttt cttaaatttt
      781 gtttttgaag gactagtatc tggagataag tatggatctg ctgtagggat cgctgcattg
      841 atccttgttg ttggaggtgc ttttggtatt attatgcgta ccggagcgat cgacgcaggt
      901 atctatgcgt ttatcagtaa aactaaggga ctggagcgtt tagctttacc gctattattc
      961 tttgcttttt cattcggtgg cgctacattc gggatggcag aagaggtaat tccattctca
     1021 atggtgatgg ttccatttgt cattgcgtta ggatatgact cgatcgtagc agtaaccgtg
     1081 acttatgtcg catctcaagt cggaaatgcg acctcttgga tgagcccgtt tagcgtagcg
     1141 gtggctcaag ggattgctgg aatccccgtg ttgtctggtg cgacattccg attgattatg
     1201 tgggtagttg ttactgcatt agccgctggt tatatgatga tatatgcaga aaaaatccgt
     1261 aaaaaaccag aaagttcgtt gacttacaaa tcagatgatt atttccgttc tcatatcaaa
     1321 aagacatctg atgaaaataa aaaattcatg cttgggcata aattgatctt attagagatg
     1381 ttagttgtcc ttgtctggat cgtttggggc gtgactcaaa aaggctatta cattccagaa
     1441 atcgcttctc aattctttgt gatgggatta gttgctggaa tcattgctgt tcttttcaaa
     1501 ttagatggaa tgaaagtcaa tgacatcgct tcttctttcc aatctggtgc agctgattta
     1561 gctggtacag caatcgtagt aggtatggcc aaagggattc ttcttgtctt gggaggatct
     1621 gatgcatctg ttccttcagc tttgaatacg attttgcatg ggatcgggac ggcgttgacc
     1681 ggtgttccag ctgtcattgg tgcttgggcg atgtacatct tccaaagttt gttcaacttg
     1741 gttgttacat caaattcagg acaagcagct ttgactatgc caatcatggc tccactggca
     1801 gatttagtag gtgtatccag acaagtagca gttcttgctt atcagcttgg ttcaggtttt
     1861 atggacgctt ttacacccgt atcagccagc ttgattggtg tgctaggtgt tgcacggatc
     1921 gaatgggcaa aatgggcaag attccaaatc aagatgcaag gattcctgtt tgttttagga
     1981 actattttca tcatgattgc cattgcaata ggtctgcagt agtactggag acctgtaaat
     2041 aaaaaagtaa tccaaacagt agataaggac tccaagaacc gtcattttac ggtaaaatca
     2101 aaggagtaaa gatcctatcg tttggattat tttttaagat cctatcatca gttatactta
     2161 aagaaagagg aaaaatgtat gaaaatcatt cgacaaattg aagtgtttgc accagattac
     2221 ttaggaaaaa tggatgtact gattgccggt gggaagattt tggctgtaga agaacagcta
     2281 gaaggcggct acgaaggagt ggaagtagaa gaactttcag gtgagggaaa agtactaacc
     2341 ccgggtttca ttgactgtca tttccatatt ttaggtggag gaggagaagg cggttatcaa
     2401 aatcgaacgc cagaagtcac attgagccaa ttgactacag ctggtgtaac gacagttgtc
     2461 ggttgtttgg gaacagacgg agaaggtaga gatatgactg cgttgatcag caaagcgaaa
     2521 ggtctggaag cagaaggaat ctccacttat gtatacgaag gttcgtaccg attgccggta
     2581 aaaacagtaa cagattctat catcaaagac tttttgacaa ttgataaaat tatcggaatc
     2641 ggagaaatcg cagtatccga tcaccgttct tctcagccaa gttttgaaga gttcgctcgt
     2701 gcagtagcag atgctcgtgt aggaggcatg ttatccggaa aagctgggat catcaatgtc
     2761 cacttaggcg gaggaaaaag aaagctagag ctattgacac gaatcgtaga agaaacagaa
     2821 attccgatca cacaattttt gcctactcat gcaaatcgca caccagaatt atttgaagct
     2881 tgcgtggcat ttgccaaacg tggtggcacg attgacttca ctgctagcga agatcctgat
     2941 ttttgggaga agacagatgg tgaagttcgc ttcagcaaag cactgaaacg attgatcgag
     3001 gaagatgttt ctttagataa tttcacgatg acctcagatg gacaaggaag cttgccttat
     3061 tttgatgaaa acaatcattt cctagggtta ggagtaggaa gtgccaaggc attgctagta
     3121 gggatcaaag aagctgtgca aaaagaaagt atccctttgg aaatcgcctt acgagctata
     3181 accagtaatc cagcacgtat attgaaattg gataaaaaag gaaaaatcga aattggtgct
     3241 gatgcagatc tgtgtatctt ggataaagaa acgcttgata ttgatacagt tatcgctaaa
     3301 ggagaaataa tggtccaaga aaaagaagtc aaagtatggg ggactttcga aaaaagtttt
     3361 taatcaacag ctctaactat cttttttaga aaacgagcgt tctgtttgtc tgtatctgtt
     3421 gttgatgtta ccctgaaggt agatcaataa acaaactaag aggaggaagc acaatggcag
     3481 atttaaaagg acgttctaaa gatatgaaag ataaagtagt cggtgaagga aaagaagcat
     3541 acggcaaagc aacagacgac aagagcaaag aaacagaagg gaaagcacaa tcactaggtg
     3601 ctgacatcaa agaaaaagct cgaaatttag gcgacgaagt caaagacaag ttttctcgaa
     3661 aagacgaaaa aaaataaaaa ccacttatta ctaattgaat caagcaaata aaaaatccga
     3721 accatatctg cgagacagat atggttcgga ttttttattt gatcattatc ctgttttttt
     3781 aaacttcgat tttttgaaat tttttatttt ttagttcaaa gacttcatca cagctttttg
     3841 ctacttcaat ctcatgagtc acgatgataa tacagcgatt ttgttcatgg gcgatttctt
     3901 ggaaaagttt gacgatatct tgtgaagttg tttcatctag atttccagta ggctcatccg
     3961 ctacgattag ttgatgttca cagcagatag cacgaacgat agcgactcgc tgctgctgac
     4021 ctcccgaaag tttggtcacc tgtttttttg ctaatatttc atcaattcct acacgagcca
     4081 gtcgatctaa agcaacttgt cgataattct cttgcttggt cttagtgatt tccattgctg
     4141 ctatgacgtt gtctagagcc gataaataag gaagcaagtt gtaagcttgg aaaataatcg
     4201 agacatcatt tttacgataa cttcttaacc cgattttgtt cagtgaacga tcttgatagt
     4261 atacctttcc ttcttttggt gcatctagtc cagaaataag cgaaagaaaa gtcgtttttc
     4321 cagaaccact tgctcctaaa atagcgtaca tttttccagc ttcgaaattc agttctacat
     4381 ctttataaag cgcatcttgc ggatcttgat accaaaatcc caattgttct gtccgtaaca
     4441 tcaaattcct cctttagttt aataggattt ttcttgggtt taatcttaat atccctatag
     4501 aagctaaaaa gacagaaaac agactgattc caaatgctac tccggctaat agtcccaatt
     4561 gtgcaggttt gacattgatt tccaattcgc tgacttgatc agaaacagca aatggattat
     4621 tcgtggttcc ttgttgacca tcgcgttgac cgccttgttc ttgtctgtta cctggcattt
     4681 gtccaccgcc aggagcttgc tggttttgtg tcgtggtttc tgtctgttgg tcaagtagct
     4741 gctgtcccac ggcattcgcg acgacgtttc cgctaagcga agcaactcct aatgcaagaa
     4801 tcatacaaat agccacttcg gtaaacaatt ggagaatgat tttacttctg gattctccta
     4861 aagatagcaa gacgcctaac tcatgccgac gctcccgaat ggtaatcata atgatcagag
     4921 taagtatgat aataccggct acagccacta gtaaaacaac atttttagaa aaacttgcca
     4981 cgttgtttaa aggttccaac attgactgat acatggaatc atttgattgt aagctaaatg
     5041 tatctgtgtc gatcagcttc tcagcttctt tgagaaactc atccatttta tctggatcag
     5101 atagagtgta aatagcagaa tctatcgtgt tatcgtcaga tgtcccattt agttcatttg
     5161 caaaagtgta agaagtgtaa agtgtattcg acggattcat gaagttgaat ttcataccca
     5221 tactgccgct tgtttcgctg gattcataaa ttcctttgat cgtcatttca taagtggtat
     5281 cttcatcttc agtactcgtg attgtgaaag tatctccgac tgccaggtca tttgtttctg
     5341 ctagagtaga atcaatgact acattgtttg tgcctttgtc gctgtcatct attccttcac
     5401 catccgtgat ttttgctgtt ccatcagaaa aattactatt ttgtgcagtt gctgacacac
     5461 cactgatttg aaagtctgct tgagtcattt gcggattccc gcccattcct ccagcaggtt
     5521 caccgcctgc atcagtagtt tctgaactgt cttcacttga atcagaacta gtgatggcag
     5581 ttattccatc ttttgccata gctgaggcgg aactttcaaa ggaataactc tttacattat
     5641 ctaattcagc tattttttcc gcgtccgtta aggaaactgg tgtaagagaa aaactgcctg
     5701 gatctggacg ttcttcagtg gtagaaccgc ttgaagaagt ttgttcctgt tttttaaatg
     5761 aagcttctcg attggcactt aaagtcactg ttgctccaac ttctttttgt gcttgattcg
     5821 ctgcttcttc tgcagcgctt cgaatcgtga ggccagctag cacaaaaata agaatggctg
     5881 tgaacacggc aaacaataac aatgaacgtc cccattttac ttttgtactt ttccatgcac
     5941 gtttcataaa attcatattt tgcatccctc ctagtagatg catttgataa tagacaacct
     6001 atcttaaagc tagcttaaat cttatgctgt ttgcacacca tcaacatgag gttctgtata
     6061 tataaaaagt cactatagtt attgcaaaaa atgtggtaaa atgaaaaaag tatgtttcaa
     6121 accgacgaag aagaaatgaa agaggaatag caaaagaaat cctagatgct acagagggat
     6181 ttttacgtgc atagttgttc taacaattgc tgatactagc gatttagttt atttgttgta
     6241 taataatggg gaacagctga atacttatat aagtattctg gctatagcgt ttcgcaaaag
     6301 atccattttg ttaatgaagt atatccaaaa taaaatgccc atccaactgt cagaacgcgg
     6361 atgtgctggt aattttcctg ctgattctcc ataagttgag aaatagtgat tttgcatatg
     6421 cttcattgga atggctcata tatttagata aagaagaaaa tcgacatctt taaacttgga
     6481 tgtcataaga agaggtgaag tttgtgagta ccattaaaat tatcccatta ggcggcgttc
     6541 gtgaaaatgg aaaaaacatg tatgtcgcag aagtgggcga ggatattttt attttagatt
     6601 gtgggctaaa atatcctgaa aatgagttgt tggggatcga cgtcgtaatc cctgatttta
     6661 cgtatttaga agaaaacatt gatcgtgtag ctggtgtctt tttaacacat ggacatgcag
     6721 atgcgatcgg ggcacttcct tacttgttgt caaaaatttc tgtaccagta ttcgggacag
     6781 agttgacgat cgagttagca aaattgaatg tcagtcgtaa tgaagcagcc aaaggattta
     6841 aggatttcca tgtggtagat gagcatacag aaatagactt tggtgaaaca gtagtgagct
     6901 ttttccgtac cactcatacg ataccagatt cagtggggat caatctgaaa acaaaagaag
     6961 gtagtatcgt ttatacggga gattttaaat ttgaccaaac ggctatccca atgtatcaaa
     7021 cagattatgc acgattggcg gaaatcggca aagaaggagt cttagcctta ctaagtgaat
     7081 cggcaaatgc agaaaatcct cagcctgtag cttctgaagc ccaaatagca gatgaagtat
     7141 ttgatacgat caaatattgg gaagggcgta tcatcgtcgc ttgtgtagca agtaatttgc
     7201 aacgagtgca acaagtattg gatgcagcag aaaaagcaca acgaaaagtt gtcttgactg
     7261 gtcaagattt cgaacgaatt attcgcacag cgatgcgttt agaaaaactt caattaccaa
     7321 gtgaagatct gcttgttacg ttgaaagaca tgaaaaacta tgatccagaa gaactgatca
     7381 ttttagaaac tggccgaatg ggtgaaccca tcaagtctct gcaaaaaatg gcaaacggta
     7441 cacaccgtaa tgtcaagatt gaagaaggag atttggttta tatcactact acgccaagca
     7501 tcgcgatgga aaccatcgtg gcgaaaacgg aagatatcat ttatcgagct ggtggaacag
     7561 tgaagttgat ctctgagaat atgcgcgttt ctggccatgc caatcctaat gacttgcaat
     7621 taatgatcaa tttgatgaaa ccaacatatt ttgtgccagt tcaaggagag tatcgtgaat
     7681 tagctgctca tgcagatttg gctcatgcag tagggatgcc atacaaaaat atctatatta
     7741 ctggacgcgg agacgtgctg gaatataaga acaagcgtat gagtgttgca ggaactacaa
     7801 gtgcagaaaa tgtaatgatt gatggattag gcataggtga tatcggaaac attgttttac
     7861 gtgatcgaaa aatcttgtca gaagacggga ttttcgtagc ggttgtcact atcagtcggc
     7921 gagaaaagaa aatcatttca aaacctcaaa ttacttcacg gggattcgtt tacgtaaaag
     7981 ctagccgtga tctgatgaaa gaaagcagca atatgattga aacaatcgtt gagaaacatt
     8041 tagaaagcaa tgattttgag tggagcaaat taaagcaaga catccgagat caattaggcc
     8101 gttacttatt tgaacaaaca aaacgccgcc cagtgatctt gccagtcatt atggaagcta
     8161 ctcaacgtcg aaataaaaaa taaacaaaaa gagtaagatg aacctctcta tagagtgctc
     8221 gtcttgctct ttttttgcga aaaacaaata atttttgaat acagcatccc tcgctttata
     8281 ataaacgata gaataaaggg agtgaatcaa tgatgagtca aacaaaagtc gaacgttatt
     8341 tagaagaaca aaagatcagc tatcaaccac ttgattttag tcatgtggta gaagaatctt
     8401 tcacagaaga actacaaaaa agagggattg atccagcttt gatatgtaaa actctcgtag
     8461 tgaaaggaaa caaaactggg gtgatcatcg cattgattcc tttgaacgat cacttagatt
     8521 acaaaaaaac gagacaagtg acgaaagatc gtaaagttgg ttttcctgga atggattttg
     8581 tattaatgca tacaggatat ccccacggag caaacacccc tattggaata aaactcgcac
     8641 atcctgatta ttgtttttta gcagacagtt ctttaaaaga aaaagaagaa gtaattgtgt
     8701 caagcggtga aatcgggaga agccttcgga taaaagtagc cgatttagaa aagatcgtcc
     8761 atcctattta cgctgattta attagttaag ggacgagaag agaagctaag cagagcagag
     8821 acctcaaact tgagattata tctgcttggt acttggtgca tcctgtatta tctgttacaa
     8881 ttgctttgta catactgtac tatcaatcca atgaaaatat atttagcagc tatatttttt
     8941 cattgatgtg caagtgatga aatcgttttt aataaaaaag atacagagaa aacgcgaggt
     9001 gtaaaatatg aaaattacgt atcatggaca ttcctgttta acaattgaaa tggaaaatgg
     9061 ccaacgcgtg atgattgacc cgtttattac aggaaatcca ttgacagact taactgcaga
     9121 agaagtaaag acagattggt tgctgattac tcatggtcat agtgaccaca taggagatat
     9181 gattcctatt gcaaaagaaa atcaagccac aattatcagt attgttgaga ttgctgatta
     9241 tgcgaacagc agaggagtag attcattcgg tatgaatatc ggtggaaaac atgcgtttcc
     9301 attcggcgtg gtaaaattcg tacatgcaca gcatagttca agctatgaag tagacggtat
     9361 cgttcagtat atgggcgagc ctagcggaat tattattcaa gcagaaggaa aaacgattta
     9421 tcatgcaggg gatactgctt atttcagtga tctaggattg cttgcagaag aattcgatat
     9481 tgatgtcgct ttcttgccga ttggcgataa ctatacgatg gggccagaag atgcagcaaa
     9541 ggctgcgcaa acaattcgtg ccaagaaagt agttccaatc cattacaata ctttccctgt
     9601 cattgaacaa gatcccgaat cttttgtaga gttactggaa gatagtgaag gaaaaataat
     9661 gagcgtctcg gaaacgctgg aagtgtaggc agaaaatggc gacaaaacat gatttgattt
     9721 tagaatatat tgaaagttta cctgttggca atcgaatttc tgtaagaagc atcgcaaaag
     9781 aattgaatgt cagtgaagga acagcgtacc gtgcaatcaa agatgcagaa aatgtcggac
     9841 tagtttcaac aattcaacga gtagggacca tccgtatcga acgcaaatta aaaaaacata
     9901 ttgaacgttt gacatttggc gaggtcgtca ggatcattga aggggacgtt ttaggcgggg
     9961 cggcaggatt agacaaggtc ttgaataaat tcgtcatcgg cgcgatgact aaacatgcca
    10021 tggaacgcta tattacgcca ggatcactaa tgatcgtcgg caaccgggcg gaagtccaaa
    10081 aattagcatt ggaagatggc gccgcagtat tgatcacagg aggattcaat acgactgctg
    10141 agattgcaaa gctagctgat gatcttgaaa tgccgatctt acgcacctct tatgatacat
    10201 ttacagtcgc aaccatgatc aaccgagcgt taagcgacca attgatcaaa aaagatatca
    10261 tgcttgtcag tgatatctat atgccagctg aaaagactca ttatttgcag cagacagata
    10321 cgatttctga ttatcagcgt ttgtcagaag aaacgcagca ttcgcgtttt ccagtcgtca
    10381 accgccatca tcgtttgatg gggattgtta cggcaaaaga tgttttaggg aaatcgccaa
    10441 accagatcat cgatcgggtg atgacgaaag aaccaatcag tgtaaagaaa acaatgagta
    10501 ttgcctctgt tagccatcaa atgatctggg atgggctaga agtcatgccg gtcgtttccg
    10561 atgacttgac attggaaggc ttgattaccc ggcaagatgt aatgaaagcg atgcagcttg
    10621 tccagcgaca accgcagatt gcagatacga tttcggatca gatatctgga aatatccata
    10681 cgattgatac tgaccgtgaa ggaaatcgtt tggaaaaccc taagttcaaa ttcgtagtag
    10741 caccacaaat ggtcaatggc gttggaacca tttcttttgg cgtacttagc gaaatcattt
    10801 ctgatgtcgc acaaaaaaca atggtgatga accaaaaacg aaatatcttg attgaacaag
    10861 tgaacctaca ttatttgcgg ctgatccaat tggaaagcga attagacatt cgtccacgtg
    10921 tattggaaat cggcagacgt tccgctaaac tggatatcga agtgtttatt gaaaacacca
    10981 tcgtttccaa agcaatcgtt gtttgtcaag taatggaacg ttcttagaaa gcaggagtga
    11041 aatgacagta cagcaagaaa tagtagatac aataaaaaaa tacgataaaa tcattatcca
    11101 caggcatttg cgccctgatc ctgatgcatt aggatcacaa gtaggattag ccgaactatt
    11161 acgggaaagt ttcccagaaa aagaaatacg tcaagtaggt gaaacgattg aagggcttcg
    11221 ctttttagca gaaatgcaag aagttgaaga tgtcttctac caaggagcgc tagtcatcgt
    11281 gactgataca gctaattcac ccagaatcag tgatcaaaga tatcagttgg cagaaaaact
    11341 gatcaaaatc gaccatcatc ccaatgatga cccttatgga gatcttgtgt gggtaaatac
    11401 aaaagcaagt agctgtagtg agatgattgc agaatttgct ttgatgtttc ctgaatcttt
    11461 aaagatgaac gttaaggctg cacggttact ttatgcagga atcgtcggag atacagggcg
    11521 ctttttgtat ccctccacga cgtcccgtac attagagatt gcttcgatat tgagaaatta
    11581 tccatttgat gcaagtgctt tgaacagaga gattgaacaa atgccgatga aagtcgctaa
    11641 actttccggt tatctttatc aaaatatcca ggtggatgaa aatggtgcag gaagaataat
    11701 ccttccgcaa gaaattcttg ataaatatgg aatcgatgat tcagagacag cttcgatcgt
    11761 ttcacttcct ggagtgattg acgaagtgct ggcttggggg atttttgttc agcagccttc
    11821 agggtattac cgcgtacgtt tacgttctaa ggggccgatc atcaatgaat tggctaaacg
    11881 ccatcatgga ggaggccatc cattagcaag tggtgcgaat gcaaaagatt tagcagaagt
    11941 agaggaaatc tataaagaga tccaagatat atgccaatcg tataagagca gaaaaacaga
    12001 atgatacttc gttcgaaaac gtgccttttg tagtaaaaat cacaaatgta tcttttcaag
    12061 atcttctttt tccgttacaa tagtgctcac taaagatttt aaaggagaaa gaagatgaca
    12121 ttaccaaatt gcccagaatg tggctcagag tacacatatg aagatcgagg cttgttgatt
    12181 tgcccagaat gcgggaacga atggaacaaa gaagaacaag aaactgtcat agaagacgga
    12241 ttagtggtat tagatgcaaa tggaaaccgt ctgttagagg gcgatagcgt gacagtgatt
    12301 aaagacttga aagtaaaagg tgcgtcaggt gccattaaac aaggaacaaa agtcaagaac
    12361 atccgtttag tagaaggcga tcataatatc gattgcaagg tagaaggatt tggaccgatg
    12421 aaactgaaat cggaatttgt gaaaaagaat taaagaatat tcaaggtctt ttcacattct
    12481 tttcctacta ctaatgataa aataaagtaa acgaaaagga gtgaaacgaa aatgtatggt
    12541 tatggaatgt atggtttttt agatccgaca tttcttttgg tcattcttgg attagttatt
    12601 tccggaattg cgtcagcgta cgtcaatagt actttccgaa aatacgatca agtgagaagc
    12661 aaaaacaatg tgactggtac acaagcagca caatatatat tacaaagtca acagataaat
    12721 aatgtcggtg ttcaacaaat tgccggagat ctcacagata actacaattc aggtaataag
    12781 atgttgagtt tgtctcaagc aactgcacag tctacttcag ttgctgcgat aggtgtagca
    12841 gctcatgaat gcggacatgc ggtacaagat gcttcgggtt atgtcccatt gaagattcgt
    12901 gcagcacttg ttccagcagc taactttggt tcgatgattt cgttcccctt gattatggta
    12961 ggtgtattgc ttagctggaa ctctacgtta atcaatatcg ggattttcgc tttttcttta
    13021 gcattacttt tccaattggt gacattacca gtcgaattta acgcgtctcg gcgagcaatc
    13081 caaattttga gtgaaggcgg actactgacg gaagaagaag taccgatggc aagacacgta
    13141 ttatttgcag cagcactgac ttatgttgca gcagcgcttt cgacattcct gcagctgttg
    13201 cgcttgatct tattgttcgg cggcaaccga agagattaaa ataaagaagt tgtgaaatca
    13261 gcgctttgat ctgagctaat accgagagtt agctgattga ataccattta tttggaataa
    13321 gaggatgtga caagaatttt gtcacatcct ctttgttttg ttctcataac cttttttcat
    13381 atacagttca tgaaccgctt ttattaactt gccaaataac actgtattgt gcgttgtatc
    13441 cagatagaaa gtctccgttt catctgaaat tttctcatgc tgatctgtaa tgatcaaatc
    13501 agcatgttct gaatatgcgg taattttaac agcatctttg cgaaataact gctgcaaatg
    13561 gctttgaaga tgataatttc ctgtaaactg tttggttatc tgcaaatgga tgagtaactg
    13621 ctctttcgtt tctgtttgta gtaagtgttg gatcaattga ctaaggtatt gactgatttg
    13681 gtttctttcc ccattttttg aaaaaattgt atcgattttt tctcttaatt tcttttcctg
    13741 tataaaagaa ctagaatgat tggacgtggc ttctggaaag caaagtgtag aaaaactact
    13801 gctggattca cccaacagtt cgaaaaaaac aactgccaac acaagaaaat aaaaataaat
    13861 tggtatttta ttttctaaat ggttgagagg aaactgttga gttaagtaag tcatcaattg
    13921 accgacttgt cgacaaataa ggtttttgct tgtggaaaat aaaaaaccaa tctcctgttt
    13981 ttgtcgaaaa gagatttgat ttggaaaaaa acaatagaaa aaataattaa agtaggctct
    14041 ctctgtttct gtctcttcac aagggcgact cactgaaaaa gaagtaaagt attctgaata
    14101 atcatgtgta tgctgcaata agtccatcaa ttcagtgatt tcagaagaag tttttggaaa
    14161 atatgcctgt tgtttgtttc gtaatatgaa taaagttaac agcaagtaga tatttttttc
    14221 ttgttttgaa attttttcta tttgctcatc tgtactgttc atttttagtg catttaattc
    14281 aagtgaggaa aatggccaat cgttacctcc aaatgtatct gaaagaaaaa agtaaagata
    14341 gagacggata gataactcat ttccttcaag agtacagcaa ttttttacag ataggatccg
    14401 taaatggaat tttattaagg aatgattgag ctgttttgtc agtttttcga gataggaggg
    14461 cgatatataa agttgttcaa gtaaatattt ttttggtaca gcagtttgtg gataattgat
    14521 tagtaacatc aaatagttaa aacgattgga ttcttttagg tagcataatt ttaacttaga
    14581 aaaaacagct aactttttag tttgatcggt ttctttggtt tggatctctc ctttatgatt
    14641 agaataaata aattcatcaa tcaataaaaa ttgcttgagt gatacattta tttttcggat
    14701 cagttctttg attgtccgtt ttggaatatg taagtcattg gataattctt gtaaagaaca
    14761 attatttttt tgaatgacca tttgtagaac aagtaactct ttttttcttg cttcaattag
    14821 cataaatacc tcctttttat tctgtttttg tatatagtta gacaaaaact gtgagtggaa
    14881 agtcatttca gtctgtgtta ttctcacaga gtgtcgcctt cttttttata taacgttatt
    14941 taattttttt gttaccttgt ttattctatt gtttatttcc tagggtattt tgtcaagata
    15001 ttcttgtttt taaggcggaa aaattgattt ttaatggata taactaaata tgttttgata
    15061 attatcaata atatttttag aattatttat gttatttatt tatgggtaga tgaaaagaga
    15121 cggaggtaag aatgatgggg agagtaacaa attatagtaa agaatatttg ctgttcaaaa
    15181 gtatggtata cgtggaagaa tatggaatga agtcaattac tgcaagagag ttagcagagt
    15241 tctgcggttg ttctacatac ccgatttata cacatttcaa atcaatcagc gggttaaaag
    15301 agaagatatt agaagaaatc actgtttatt ttgaaaacta cttagctgaa tgcagttccg
    15361 atgatgtttt aagcattgtc tacttattga aagatttttt ttctatccat gaaagtattc
    15421 gtgaaatcat tgcaaaatct gatttagaca tggattcgct atttaagcgg ccatttttct
    15481 catatgtcaa aggtcacacg aagatcaaag aagaaaagtc gcttgaattg gtatggctta
    15541 atgcattggg aagtttgcgc tcagatgatt cttctcaaga ttttcttgaa atgatgaacg
    15601 ctgtttggga taaaaacgag gtgacggatg aatacttgga aaatatctta aaggaacagt
    15661 caccttaggc agcaacatga aaaagtagat cagccacctt tatttttcgt aaaaaagatt
    15721 gtaaaatgtc taacgaaaca ttggaatgaa ggaggaagcg acaacgttac cacagcaatt
    15781 tatcaaaaaa tatcgtcttc ttttaggcga agaagcatcc gatttttttt ctgcgcttga
    15841 acaaggaagc gtgaaaaaag gatttcggtg gaatccacta aaaccggcag gattggacat
    15901 ggtccaaact taccatagcg aagaattaca acccgctccc tacagcaatg agggatttct
    15961 tggcactgtc aatggaaagt ctttcctcca tcaagctggg tatgaatata gccaagaacc
    16021 aagtgcaatg atcgttggaa cagctgctgc agcaaaaccc ggtgaaaaag ttttggattt
    16081 gtgtgcggct cccggtggta aatcaaccca attagctgct caaatgaagg ggaaagggtt
    16141 attagtgacg aatgagatct ttccgaaacg tgcgaagatt ttatcagaaa atatagaacg
    16201 atggggagtt tcgaacgcaa tagtgacaaa tcatgcaccc gcagaacttg tccctcattt
    16261 ttcgggcttt tttgatcgga tcgtggttga tgctccttgt tcaggagaag gaatgttccg
    16321 aaaagatcca aatgcaataa aagagtggac agaagaatct ccgctatact gtcaaaaacg
    16381 tcagcaagag attttgtctt cagcaataaa aatgctaaaa aataaaggac aactgattta
    16441 ttcgacttgt acgtttgcac cagaagaaaa tgaagagatc attagctggc ttgtagaaaa
    16501 ttatccagta acgattgagg agattccttt gactcaatcc gttagttcag gacgttctga
    16561 atggggaagc gtagctggtt tggaaaaaac aatacgtata tggcctcata aagatcaggg
    16621 tgaaggacat tttgttgcaa agctgacttt tcatggacaa aatcagatgc ataaagaaaa
    16681 gaagacacga aaaaaaagta aagtgcaaat gacaaaagag caggaaaaac tctggacgga
    16741 gttttcaaat gattttcatt atgaagctac tggaagactt ttagtgttca acgaccatct
    16801 ttgggaagtt cctgagttag ccccttctct cgatggttta aaagtcgtac gtacaggttt
    16861 gcatttagga gatttcaaaa agaatcgatt tgaacctagc tatgcattag cgcttgctac
    16921 aaaaaagata gagaatatac cttgcttgcc gatcactcaa aaggaatggc aatcttacac
    16981 agcaggagaa acatttcaac gtgatgggaa tcaaggatgg gttttgctag tattagataa
    17041 gatacctgtc ggatttggga aacaagtaaa aggaacagta aagaactttt tccctaaagg
    17101 attgagattt cactgagtgg attagttcgt ttattttttt gtctatgcta tgatggagat
    17161 agataaaaag gagctgatac ttatgaaaac atcaacaaaa gtaacaatcg gattaagcgt
    17221 agcagccgct gccagtgtag cgacagcagt cgttgtatcg ggaaaagtaa tggaaaagat
    17281 ccatcatatg actactcgga cgaaagtaaa aaaattcgtc tatgacaaat ttgatggaaa
    17341 tgaaaaatta ctagaaatcg tagatggtct ttcagatagt gacttggatt ctttgatggg
    17401 aatgttagca aaaatcaaat caggaaaaaa gaaaatctct gtttatggtg attcaatcaa
    17461 agattcaacc gaagatgtaa aagaccggct aatgcatttt gttgaaaaaa tgatgtaaga
    17521 gaaactagct tttgaacact attcattcga aataaggggt tgtgacaaaa ttattgtcac
    17581 aaccccttta atctatcttt aattatgttc atgaagcaat aaaagatagc aaaacagtaa
    17641 atcgttggct ttttttagat tgaaaccaga ctgttcttga aatttttcga ttcgatattt
    17701 tagagtatta cgatgaagaa acagtgtttt tgcagcagag ctgacattgc ctaattcatt
    17761 ccataattct tgaatgatca cttccatttc attttgtttg atcagttcta tgtaactttt
    17821 gatcaactta ctatctttta cagtatcttt tgtatagtaa tgtaaagcca catcttgcat
    17881 agaaaaagta cggctgtgag tataaagatt gttttgctct gttaaaaaaa tttttctttc
    17941 ttcagcaaac aaagggacga ggtcggtgcc aggtgaatgg aaagaaccaa cgaatacaga
    18001 cgttgtcgta tcaaaatcag catctaaaga tagaaaaata ccgttgattt cagtagcatc
    18061 aagataatca cggtttattt gttcgactaa gagcgcatct gtgtctgtat aaaaaaagaa
    18121 atcagcagaa tagcgaaata attccaaaat agtatcttgc cattcttttt ttaagaattc
    18181 tccttttgat tcgacgtgaa gctggatcaa acgaaagctt ttttcttgaa cacaagaagc
    18241 atctttaaaa agatagtgat accatgggtg ttttttttga tcgttgatta cagggaataa
    18301 cgctttcaaa aggctggttt cttgttgaga taatgaatct tgtttgatcc agataaaagc
    18361 atcatcaatt gcaaaagaaa ggatatccgc ttctgatgct ggagagtttt ttttctgtgc
    18421 ttgtgggtaa agagcaagta atttatccat tttcatcttg ttcacctctt gtatttattt
    18481 taccatagaa aaaaagttag ttacagcctt tttatcatat gctaaagtaa aacaagaaaa
    18541 aacaaaggca gggaagggta attggtaaat acaaatcttg atgcagtaga aaaagaaaaa
    18601 tggatgcgcc ttgcattggc agaagcaaag aaagcagaaa tgcttcatga agttccgatt
    18661 ggagcagtgg ttgtattgga tggaaaagtg attggaagag gatataacct tagagaaacc
    18721 acgcaagatg ctactacaca tgcagaaatg cttgcaatca aggaagcctg tgaaaaagtg
    18781 ggaagctggc gcttggaaga tgctgcactt tttgttacct tagagccttg tcctatgtgc
    18841 agcggcgcga tgattttgtc tcgtgtagca gaagtttatt tcggcgcgta tgatccaaaa
    18901 ggcggaacag ccggtacctt gatgaatctt ttggaggacg agcggtttaa ccaccaagca
    18961 tatgtagaag gtggcatttt agaagacgaa tgtgggatga ttttgactga ctttttccga
    19021 aagctccgcc agcgaaaaaa gaaattaaaa aaagactggt caacgaatga aaaataaggt
    19081 atactattct ttgccgaaag gctaggacaa tggtgggctt gtgaagcgtg tcaggtctgg
    19141 aaggaagcag cactaagcag gttccgccat gtgtctgatt ataatgaaac gacaacagac
    19201 tttcgggtct gtttttttta tttttatagt actgtaaaaa tgaatgttcc tgatttttta
    19261 gccagactga tttatcgaat attgtactta tacaatttgc ttcaaggcag gaaaaactct
    19321 gaaatagagc ttgtttcaat ggaagaaaaa gctgcctatg gtatgataaa gataaagaga
    19381 ggcgagaggg tacatggaaa aaatagtgat tttacatacg aatgatctgc attctcattt
    19441 agaaaattgg ccaaggatca gaaggttttt ggatcaacgt aaacgagaaa acgaaaagaa
    19501 agaaaaaacg acaacgattg cagtagatct aggtgatttt gtggatcgtt ggcatccatt
    19561 gtcagaagca acgaatggac aggaaaatat cttgctgatg aatgaagttg gttatgatgc
    19621 agcgacgata ggaaacaatg aaggcgtagg aaattccaaa gaagagttga atcatttata
    19681 cgatcatgct gagtttgata tcatcctgga taacttattc gataaaaata cgctccagcc
    19741 accggcatgg acgaaaccat ataaaatcat tacaacgaaa caccagacga aaatcggact
    19801 aattgcattt actgctccat ttccgcttac ttataatccg aatggctggg acatacggaa
    19861 tccttatgat attttgccag aattagtcga agatttacgt tcacaagtgg atgttttagt
    19921 tttgatgagt catctcggga ttcaggatga tttccaaatt gcaaaagaaa taccagcaat
    19981 tgatgttatt ttaggatcgc acacccatca tttattcaga gaaggtcacg taatcaatga
    20041 tgtccaaata gcagccgctg gaaagtacgg gcagtatgtc ggtgaagtcc atttaaccat
    20101 cgatgaagag aaaaaaatcc ttaagcattc agccaaagcc atccctaccg aaacaatgac
    20161 agcttttaca gaagatgaac aagaaataaa tagttgtcta gaacgtgggc atgaattatt
    20221 aaaagaaaaa gaagtagcag atttgccatt ttctctgagt ctagatatct tcaatgagca
    20281 ttcgtttatc caagtagcct ttgaggcggt gaaagaacga ggaaagacgg aagcagcaat
    20341 attaaacagt ggtctgtttt taactgaaat tccaaaaggg cgggtcaatc aggatcagct
    20401 tcacactgcg ttgccgcatc cgatgcactt gttaaatgtc acattgggtg gaaatgatct
    20461 gatccgctta gttttagaaa tagaaaaaaa tcgtaacttt ttacgtaact atcctatgaa
    20521 gggaatggga tttcgcggta agatttttgg gcagatggtc tataatggaa tcacatatga
    20581 ttctattaac catcaagtct tttggaagaa caagccaatc gatctgaata aaaattatac
    20641 ttttactacc gtagatcatt tgatgtttgt tccatttttt ccaacgattg agattgctgg
    20701 aaaaaatgaa tttttgttcc ctgaatttat ccgaagtgtg gtaggagatt acttgaaagc
    20761 tcactaccca atcaaataaa aacaaggtat aataaaaaag ataggtggtg aacgcatgtc
    20821 tgcaaaacaa aatgaacagc agaacgttga gcataaaaca gaaattgcta agaaaaaatc
    20881 atcagctgaa caggaaacaa cagtcaccga agaactgaca gctgctttgg aagaaatcgt
    20941 tgaggagcct aaagcagaaa aagtcaaagg cgagctagtg acactgttag atgacagtaa
    21001 ttttttgata ggtgaaagac attatcggct tgtgtccgat tatcgtgaag gatttaatgc
    21061 agaaaagctt ggagaacgtt acagtgatgt actagcccga tatgattata tcgtaggaga
    21121 ttggggttat gaacaactac gcctaaaagg atttttccaa gcggataatc gacgggcgca
    21181 tccagatcag cgaatcgatt ccttggaaga ctatctttac gaatattgca attttggttg
    21241 tgcgtatttt gtcatcgaga gaatcggcgg taaaaaagaa aagacaactc agcgccgaaa
    21301 aaagaaaaaa aaccataatc gtgcgtatat cgatgagaaa aaaggaccag tcgcatctaa
    21361 tcgcaaaaaa ccggttatta aaaatcgaaa aacagagtca gcaaaaacct ctgaaaaagt
    21421 aaaaaaacaa gaacaaccta aacaagtaga aaaaacaaaa acagcattta cgatcagaaa
    21481 aagggaagag tagtggatga atggactata aaggatattt aattgattta gacggaacga
    21541 tttatcgtgg gacacaaccc ataccggccg gtaaacgatt tgtggaagaa ttgcaaaaaa
    21601 gaaaattacc tttcttgttt gttaccaata acacgactaa gtcaccagaa acagtagcta
    21661 atcgtctagc agatgagttt gacattcatg tagctccaga gacagtctac acagctactt
    21721 tggcaacaat cgattttatg aaagcagatg gaaaagggaa aaaagtctat gttatcggag
    21781 aagcaggatt gattgacttg attttagctg ccggatttac atgggaagaa gaggctcctg
    21841 actatgtagt ggtaggctta gataattatc tgacctatga aaaagtagtt aaagcaacac
    21901 ttgctattca aaaaggtgct actttcattg gaacgaaccc agataagaat atacctactg
    21961 aacgcggcct tcttcctgga gcagggtcag tcatctcttt tgtagagaca gcaacccaaa
    22021 cgccgccgat ctacatcgga aaaccagaag ccatcattat ggataaagcc gttgaagtat
    22081 tagggctgca aaaagaagaa gtaatcatgg ttggagataa ctatgaaaca gatatccaag
    22141 ctgggatacg caataatata gatactttgc tcgttttatc aggttttaca aaagaagaag
    22201 atgtacctgg gttgcctatc ccagctacct atgtgaaaca atcactggat gaatggagct
    22261 tctaatatga aaaattcgag atggcagtgg atggaatatg ctggattgtt cagcctattt
    22321 ctgacactga tcagtctggc agttggtgtg acgatcaact ttcgaccact atacgtattt
    22381 gatatcggac atttgcaaat tttggattat acttctttag atcaagagac gttactgaaa
    22441 aattttgatc acctaatgaa ttatctcaat aatcccttca aaaccatcct cagcttgcca
    22501 gatttcccag tttctgctag cggagcccat cacttttatg aagtgaaaat tttgttttta
    22561 gtggattatg ctgtgttttt catcactttg atcccgagta ttttgtttat caaatatttg
    22621 caaaagaatg atcggctttg gcggttgatt cgtccgtttc aaatcggcat gttactgcca
    22681 gttgttttcg gttttttcat gatgatagga tttgatcgct tttttatcct ttttcatgaa
    22741 acgtttttca acaacgatga ctggttattc gatcccgtta ctgatccaat catcaatgta
    22801 cttccagaag aatttttcat gcatagcttt atcttatttt ttgtattatt agaattattt
    22861 tttgctgttt ttcttttctt aggaaaaaat agcttgaaac aaacaaaaaa gaaagaactc
    22921 gtctaaatag acgttttctt tctttttttg ttgaattatt gagtaaggcg acttcttttt
    22981 tctggttctt tgtgtgctaa tcgactggca gcagcagcgg cgatcgctcc gacgatatca
    23041 tctaagaagg tatggacatg aacgccatcg tggctgttta gttcttttaa aataccaggt
    23101 ttcaccttgt cgatataacc ataattcgta aagccgatcg taccgtaaac attcacgatt
    23161 gacaaggcta ggatctcatc gataccgtaa aggccttcgt catcttcgat aatatgttgc
    23221 aacggactca tcaatttttt cttttctgct aatatatcta attggattcc agtgataatg
    23281 gcgttatgta cttcacgctt tgttagcacg ctgttgacac tttcagtaca atccgccaaa
    23341 gtcaaatcag aaatatagtc tttttgtaaa aacatgacaa gttgtgcgat atcttctaca
    23401 gttacgccac gttccttcaa tagttctctt gccttttctt ctaaagtctc agttttgacg
    23461 accatattat tgctccccct ttattttttc tttctctatt tcatgttaac ggaacatgaa
    23521 aaaaatgcaa gaaactgctt tcgggtttaa cttgaaatgc aaagagatat aaaaaatcaa
    23581 ctaggacgaa gatttttatc gtataagctt gtgctgtgac caggctgagt tcgttccttt
    23641 ggattaatat agtgtaaagc gttgttcact gcagtaggtg cttcaccgag cccagtagca
    23701 atcagtttca cttttccttc ataggtacag atgtcaccag ctgcataaac gcctggaata
    23761 gaagtagaca tatctgattt tacagtaatg gcatttctgg tactatcaag tccccaagaa
    23821 gaaagatgtt ctaaattaga agagaatcca taattgacga tcagagaatc aatcatcaaa
    23881 tcaatcgtct cgtcactttt cacttttttt aaccggatat ctgtcagttc cccattattg
    23941 cctgataatc catcgatgag atagggagta agcaagttga cagatgaaga tttcaagcgt
    24001 gatacactat gttcgtgcgc acgaaattcc gggcgacgat gaatcaagta aacttcgctt
    24061 gcaataggct ctaacatcaa cgtccaatcg atagcagaat caccacctcc agcaatagcg
    24121 actttttttc cagcatactt catcaaatca ttcacgaaat aatccaatcc gtggttttca
    24181 aatgattcag cattatctaa attcaatttt cttggctgga atgaaccatt tcctaaagct
    24241 aaaatgactg ctttggaata atggatgcct ttatttgtag taatctcgat aacttcgtct
    24301 tcacgagtaa gcgagagaac ttcttctttt aaatggaatg tatggttaaa agtagttaat
    24361 tgtttttcta attgatcgat taattcactt gcttttattg ctggataccc aggaatatcg
    24421 taaatatatt tttcgggata caaagttgct aattgaccac caagttgagg tagactgtca
    24481 atgatttttg ttttagcttg gcgcattcct gcgtaaaaag cggcaaacat ccctacagga
    24541 cctgcaccga tgattgtaat gtcatagatt tccataaaaa ttcctccctt ttcacactat
    24601 atcagaattt aattgagaat gcttctcaaa aatgtttgaa ttcgaaatcg aaacggtaag
    24661 ttatagaatg tatctagtga tgaaaaataa aagaaaacaa atctagtatt tttacgagag
    24721 gttaggtatt atgttattat tattatgaag tatgcaaata ttaggaggaa acaatatggc
    24781 attcccacaa ctagatttaa aaagtgaaaa aggaccaaaa gctgttatca aaacaaaccg
    24841 tggtgacatt actgttcaat tattccctga actagcaccg aagacggttc aaaatttcat
    24901 tgaactttcc aaaaaaggtt attatgatgg cgtgattttt caccgcgtta ttcctgattt
    24961 catgatccaa ggcggcgatc caactggaac aggaatgggt ggcgaaagta tttatggcga
    25021 aagttttgaa gatgaattca gtcgtgaact gttcaacctt cgcggagctt tatctatggc
    25081 aaattcaggt ccaaacacaa acggaagcca attctttatt gtaaataaca caaatgtgcc
    25141 agctaatatg cttggacaac tagaaggtgc tggattccca tcagaaatca ttgaagcgta
    25201 taaaggtggc ggaactcctt ggctagattt ccgacataca gtctttggac atgtactaga
    25261 agggatggac acagtagatg aaatcgcaaa tgtacaacgt ggtccacaag accgacctgt
    25321 acatgatgta gtgattgaaa caattgaaat ttcagaatag tttggtatgg tacggagtaa
    25381 ttatgaaaaa ggacgaaacc tagtaaggtc tcgtcctttt attttgaata aatgatttaa
    25441 tcgattgtct ctaaagcata cttgattcga ttaagtcctt ccagtaaagt ttcttctgga
    25501 caagcaatat tcaagcgcat atgcccatga ccggaaggac caaacgtgat gccaggattt
    25561 aatacgactt ttcctttccg aacaagagtg ttctccaatt cttggtcgtc tgaaatatag
    25621 gcggtaaaat ctaaccacat taagtaagtt ccttcagggt tcattattct cactttcgga
    25681 agatactgtt caaaaaactg attaactgtt tgaacatttt tttctagata aggaatcagt
    25741 tggcttaacc attctccacc cgtctcataa gccgcttggg ttccaatcag tccaaaagtg
    25801 ttgatttcgt gctgttggtt catcactaaa tgtttttcaa aatcagcttt catctctgga
    25861 tttttgataa ataccattga atttttgatt gcagctagat tgaaagtctt ggttgcggaa
    25921 gtaaaagcaa ctagtagatc atctaaatta ggatcaatcg ttagcatgga agtaaatgta
    25981 tgatcgaata aagttaaatc ttgatggatc tcgtcactaa acagaatcac ttgatgtttt
    26041 aaacacaatt cgcttaactg tttcaattct tctttgctcc aaacacgccc tccaggattg
    26101 tgaggattgc ataaaatcat tgcttttaca ttgttttctt cgattttttt ctccatgtca
    26161 gacaagtcca tgatgaaatg attatttttt tctagcaaag agcttcgaac gatgtgacgt
    26221 tgatttgttt ctataataga ggaaaatgga ggataaactg ggtcatgaat caaaatagaa
    26281 tcgcctggtt ttgtaaaagt ttgtactgct gcagctaaac tggttaacac accacttgta
    26341 aaaacgatat tttctttcgt caactccaca gcatggtgtt ggcgttccca atgaatgata
    26401 gcctgatata gtttatcaga cagagtggca taaccaaaga ttccatgttt gatgtattca
    26461 ctaaacgctt tagctacccc accgggagac aagaagtcca tatctgctac ccacaaagga
    26521 agtaagtcat ccatttgata tgtctcagca attgcgtccc attttacact ttctgtatct
    26581 tttcttgaaa tctgtttatc aaattcgatc atcttaagac ctccagtcat tttcgtcgct
    26641 tctttttttg agctatttac agtataatga taaatgcgaa gagagggaag tagatgactt
    26701 ataaaattgg acaaataatt gaaggaaaag tgacagggat acagccttat ggcgcattcg
    26761 tttctcttga taacgagacg caaggactta ttcatgtttc agaagtacaa tccggttata
    26821 cgaaaaatat ccattcattg ttaaaagtcg gacagccagt aacagttcag atcatcgaca
    26881 ttgatgaata ttccaaaaaa atcagtttat ctttaagaac gttagaaaaa acaaatccag
    26941 ctgttccata tcgcagaaaa aggtatttca cgaataaaaa tcgtaagatc ggatttacca
    27001 ctattgcgga gcagttacct atttggatag aagaagcatt atctgaacta gaacggaaag
    27061 aaaataagaa ataaaaagtg tattttttct gaaaatgtgc tagaattttt atgtaagcca
    27121 tagaacaagt tgatcgaggt ggaaattttg gcaagcaaaa gagttgctgg tacaattatg
    27181 ttgcatttgg aggatggtac taaaaaattt ttggtccatt caattgatga caagttggaa
    27241 tttgcgttag cggaactttc tgaaggaaag actggtttag cgaatatact taatttctta
    27301 aaagaaatag ttcatatcga tgttagcaag attagtttga tggaattgac aaatacacat
    27361 attaaagagg aaaatgttcc gttgtttgtc tttgaaactg aacaaaagaa tcaaagagaa
    27421 gaattaggcg aaggatatat ttgggaagaa ccaggtcaaa tgcgctctgt tcttggaact
    27481 tacgaagtag agggagtgcc tttcttctaa aatacagatt aagcgaacaa agaagtttga
    27541 atttttttca aacttctttt tttattgttt ggtccaaaaa atacaatgaa atgttctgct
    27601 aaagatagtt ttttagtgca ttttattttc aaaatatgtt gaaaacggtt gtataacgaa
    27661 taataataat tgatcgaaaa atcatttcac aaagtaatgc ttaatttata gttgaccaag
    27721 gaagaaatac atgataaatt taacagcgtt gattctgaag tggatgttat atcacatttc
    27781 taaaaaattt aaaaaa
//
LOCUS       DAZAID010000028        27536 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000028 DAZAID010000000
VERSION     DAZAID010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.028"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..995
                     /locus_tag="ACG9BE_001145"
     CDS             <1..995
                     /locus_tag="ACG9BE_001145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFN3031905.1"
                     /translation="LDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIG
                     VEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLG
                     RKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDI
                     YAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNY
                     NFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLC
                     SQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            1107..2558
                     /locus_tag="ACG9BE_001146"
     CDS             1107..2558
                     /locus_tag="ACG9BE_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN3031906.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            2907..3539
                     /locus_tag="ACG9BE_001147"
     CDS             2907..3539
                     /locus_tag="ACG9BE_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFN3031907.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            4127..5566
                     /locus_tag="ACG9BE_001148"
     CDS             4127..5566
                     /locus_tag="ACG9BE_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3031908.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            5861..6079
                     /locus_tag="ACG9BE_001149"
                     /pseudo
     CDS             5861..6079
                     /locus_tag="ACG9BE_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            6328..6543
                     /locus_tag="ACG9BE_001150"
     CDS             6328..6543
                     /locus_tag="ACG9BE_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031909.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(6760..7016)
                     /locus_tag="ACG9BE_001151"
                     /pseudo
     CDS             complement(6760..7016)
                     /locus_tag="ACG9BE_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7203..8114)
                     /locus_tag="ACG9BE_001152"
     CDS             complement(7203..8114)
                     /locus_tag="ACG9BE_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN3031910.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(8133..9137)
                     /locus_tag="ACG9BE_001153"
     CDS             complement(8133..9137)
                     /locus_tag="ACG9BE_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFN3031911.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(9134..11257)
                     /locus_tag="ACG9BE_001154"
     CDS             complement(9134..11257)
                     /locus_tag="ACG9BE_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN3031912.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(11262..13709)
                     /locus_tag="ACG9BE_001155"
     CDS             complement(11262..13709)
                     /locus_tag="ACG9BE_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3031913.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(13696..14085)
                     /locus_tag="ACG9BE_001156"
     CDS             complement(13696..14085)
                     /locus_tag="ACG9BE_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN3031914.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(14145..14648)
                     /locus_tag="ACG9BE_001157"
     CDS             complement(14145..14648)
                     /locus_tag="ACG9BE_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFN3031915.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(14661..14885)
                     /locus_tag="ACG9BE_001158"
     CDS             complement(14661..14885)
                     /locus_tag="ACG9BE_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031916.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(14989..16899)
                     /gene="ltrA"
                     /locus_tag="ACG9BE_001159"
     CDS             complement(14989..16899)
                     /gene="ltrA"
                     /locus_tag="ACG9BE_001159"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFN3031917.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(17645..17782)
                     /locus_tag="ACG9BE_001160"
     CDS             complement(17645..17782)
                     /locus_tag="ACG9BE_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFN3031918.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(17779..18963)
                     /gene="mobT"
                     /locus_tag="ACG9BE_001161"
     CDS             complement(17779..18963)
                     /gene="mobT"
                     /locus_tag="ACG9BE_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFN3031919.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(19222..19512)
                     /locus_tag="ACG9BE_001162"
     CDS             complement(19222..19512)
                     /locus_tag="ACG9BE_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031920.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(19502..20644)
                     /gene="dcm"
                     /locus_tag="ACG9BE_001163"
                     /pseudo
     CDS             complement(19502..20644)
                     /gene="dcm"
                     /locus_tag="ACG9BE_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(20720..20884)
                     /locus_tag="ACG9BE_001164"
     CDS             complement(20720..20884)
                     /locus_tag="ACG9BE_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031921.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(20877..21551)
                     /locus_tag="ACG9BE_001165"
     CDS             complement(20877..21551)
                     /locus_tag="ACG9BE_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN3031922.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(21629..22969)
                     /locus_tag="ACG9BE_001166"
     CDS             complement(21629..22969)
                     /locus_tag="ACG9BE_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFN3031923.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(23052..23387)
                     /locus_tag="ACG9BE_001167"
     CDS             complement(23052..23387)
                     /locus_tag="ACG9BE_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFN3031924.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(23397..23771)
                     /locus_tag="ACG9BE_001168"
     CDS             complement(23397..23771)
                     /locus_tag="ACG9BE_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN3031925.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(23784..24098)
                     /locus_tag="ACG9BE_001169"
     CDS             complement(23784..24098)
                     /locus_tag="ACG9BE_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN3031926.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(24211..27438)
                     /gene="fss3"
                     /locus_tag="ACG9BE_001170"
     CDS             complement(24211..27438)
                     /gene="fss3"
                     /locus_tag="ACG9BE_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFN3031927.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         8066 a         5656 c         4797 g         9017 t
ORIGIN      
        1 tgctagatat accggggaat aatttagcta atattttttc gataaagaat tttccaaatg
       61 caaaagagat ttttaaagct ttggatgata gtactattaa aaaagtagga attattggag
      121 caggatatat tggagttgaa attgctgaag ccataagaaa aagagggaaa gaagtatatc
      181 ttttcgatgt tgcagataga gtgttgtcga cttattacga ccgtagtttt tcagataaag
      241 ttgaagaaat actatcaaaa aatggaatac atctttgtct atcagaaagt cctaaaaaat
      301 atttaggaag aaaaaagatt gaaaaaatag tcactacaag tggtacccaa tactctatgg
      361 atatggtcgt acaagctata ggatttctat ctaatagtcc aattggggaa aaggaactct
      421 tgagagatgt tagtggagca tatctaacta atgaatacca gcaaacaaat ataaaagata
      481 tatatgctat tggggattgt gctacaactt tttttacgtc cattaataga agtagtgttg
      541 atttttctat ttctaatgct ctaagaacag cttatattgc aactcaacat attaacaatc
      601 aagattttac accttctggt tcccaattta caaatggctt ttctatattt aattacaatt
      661 tttacgcagc aggtttgaat ttaaaaacag cacgattatt aagtgagtct atagattata
      721 tagaaataga agatttcatt agacctgctt ttttgagaaa aaatagtcgt gtaaaattaa
      781 gaattagctt tgaaaaaaat acaaagagaa tagtaggtgt acaactttgt agtcaagaag
      841 atttatctgg actattatca atgttttctc ttgctataga agaacaaatt acattagaca
      901 aattgaaaat gttagattat ttatttcatc ctagttttag ccaaccctat aattttattt
      961 taaaggcagt aacaggatta aacattgaaa cataatcgtt actaaatgaa atcaagaaat
     1021 atactgtatt actcaattca ttgaaaatat ttatcataat ttttaataga aaacactatt
     1081 aaagagaaaa aagagaggta aatcaaatga aaaaatcttt aaaacttgca tcagcagtta
     1141 tattaatgtc ccaactcctt atagcaacac ctttccatgt gttggcaagc gaaaattcaa
     1201 catacaaaat tgcaaaagaa agaaccgaga cgactgaaat gagtaattcc gatgtaacag
     1261 cagagagtag tgaacttttg acaacttcat caagtgaaag tagtacgact tcaaatgccg
     1321 agtcttcaac aacttcatca agtgaaagta gtacgacttc aaatgccgag tcttcaacaa
     1381 cttcatcaag tgaaagtagt acgacttcaa atgccgagtc ttcaacaact tcgtcaagtg
     1441 aaagtagtac gatttcaagt accgaatctt caacaacttc aagtagtaca agtgaaataa
     1501 cttcttcgaa agtgacaaaa ccaaaccata tagaatcttc aaaaaaggaa gcgccaaaaa
     1561 cggaaggaca aacaaaaact ttttcgacac cacagcaaat agaatcaaca gaatctttcc
     1621 aatcacaacc aatactatct ggtaatgaag aaattcattt tgacaaaaat gaatctgtag
     1681 aaaattttat tatgaaaatt ggtgagtctg cgagaaaaat tggtcagaaa tatgatttat
     1741 atgcttctgt tatgattgca caagcgatct tagaatctgc cagtggtcaa agccagttag
     1801 ctcaggcacc gaattacaat ttatttggga taaaaggaac ttataatgga aactttgtca
     1861 ttatggtaac aaacgaagat ttgggtaatg gaacgcttta tacgacccaa tcaaaattta
     1921 gagtgtatga aaactatgag gaaagctttg aagattatgc caaattatta actaagggaa
     1981 ttagtggaaa taaagatttt tatgcaggag cattaaaagc taatagtaaa acatatcgag
     2041 aagcaactaa attccttaca ggacggtatg caacagatac acaatactat ttgaagctaa
     2101 atgaattaat taaaacctac gatttaacca actatgataa agaagttaaa ggtccacaaa
     2161 tgagtaagac tggataccaa gtgcctattc ataattattc aataaccagt ttgtttggaa
     2221 atcgtggaaa tgaatttcac cgaggacttg atatggcagc tacacagggt gagccgattc
     2281 atgcagttaa agctggtaag gttgttaaag cagagtttca ctactcttgg ggaaattacg
     2341 tggtgataca acacgaagat ggatcaaccg cgttatatgc tcaccagcaa caatacctag
     2401 taaaaactgg agataatgtt ttgcaaggac aaataattgg ctatgtagga agtactggga
     2461 atagtactgg cccacacctt catttggaaa tttgcaaaga tagtagtctt tctcagagca
     2521 tgttagttga tccgaaaacc ttattattcg gagactaaaa aaggtaatca atctgacaaa
     2581 gtaacaagaa aatggttgga ttcttagacc tttgatcagg atattgagac tggaattgat
     2641 atctcgatta cgatgggttt gacaaataaa acaagtccat tatcgaatat cgagtgtatt
     2701 attgccgtct ttatgctcat attttgagca ggtttcacta gatgaaaaca atttatctat
     2761 cttgaggatt tcgtgtacgt atcgttcagt cttgtattgt aaattagtca taaaatcaga
     2821 ctaagaaaaa ttggaaatat ttttttctga tttattaaaa gttaattgaa attatatggt
     2881 acttctttct tgggagggta ggtgcagtga aggaaagata tggtcacctt actgttgttg
     2941 aggagattac tcataatgga tataaaaaat atcgttgcct atgtgactgt gggaatgaaa
     3001 cgattgtata tcgatcaaac ttactatcag gtagaaccaa aagttgtgga tgtggaaatt
     3061 ataaaaatag gcataaatat aaaaatttga caaataaaac ttttggtaag ttagttgcaa
     3121 tcaagccaac taaactgcga agtgaaggaa ctgttgtttg ggaatgtcaa tgtctgtgtg
     3181 ggagaaaaaa gttagtatcg gcaaagcgac ttctaaaagg tgatgtaaaa agctgtggat
     3241 gtttgaaatc aaaaaagcat cacattgaaa ataaaaaatt cggattactt actgcaaagt
     3301 atcctttgga caataactgt tcatggaaaa caaaatgggt ttgtcattgt gagtgtggca
     3361 gtatgtgtga gataagttat agtaacttat tttttggaca tactcaaagt tgtggatgtt
     3421 taaaaaggca agaaacaagg acatgtgttg aagggacagt aatagagtgt ctaacgtcaa
     3481 aattaccgaa aaataataca agtggtgtta agggagttta tcgttctaaa gagaaataga
     3541 ttgcgtatat aactttaaaa aaaaagaggt actatttagg atcgtatgat tctattaaga
     3601 gtgcagctca agctagaaaa gatgctgaaa ataaattgtt tcaacctttg attgacaaat
     3661 atcgaggtaa tcattaatta ttgtggataa taattaacag tgatatataa acatctataa
     3721 tatattattc atactattga tatttagtat aatcctatgg aattgaatat catagcaatg
     3781 tatttgaaaa gcaaattgga aaacaaatag gtgcaacacc ataaatatga aaaaattggg
     3841 aaaaagtaga atggaaattt gcttacgtct cacgagatag ctcacactat taatctgaag
     3901 aacaatgaag tcgactttta gaaattgcta gaagaaagta aagcgaatta tcactagtta
     3961 ctatagagta agttcaaata aagcatctcc aacttacatg gttacatgag gatatcaaat
     4021 cgaaattact caagcttcct atggctagag tcgcgagaag ttgttgggca gtagacatag
     4081 gttatattgt tcttagctac acgatcatta gaaaggaaat cctattatgt atagttatca
     4141 agaagatgca aaagtccaaa aaaatcgctg gtggattttg attgctatat ctatgtttac
     4201 cttcatgtcc acattggatg gcagtattgt caatattgca ctacctacca tctcagatga
     4261 tatgggcata ccaatgaatc aagcggaatg ggtagtgtcc gtttatctga tgattgtctg
     4321 ttcttgtttg ttgctgtttg gtaaaattgg cgatagccgt gggaaaatca aagtatttcg
     4381 gattggtacg ctgatctttg tcttgggatc gctgttgtgt gggttcaatc attcgctagg
     4441 tattttattg ttcgcacggg tggtacaagc cattggtgct agtatgacta tggctacaaa
     4501 tgccggaatc atcacggaag tctttcccat acatgaacgt ggtcgggcat tgggagcaat
     4561 aggtgcgttt gtttcattag gctcgattgc tggaccaggt cttggcggct tgattttgtc
     4621 ccaattttct tgggcgtaca ttttttggat caatgtaccg gtgggaatcg taacgatgtt
     4681 gattgctgaa aaattcttac caaaagatat caccatgagc ggccatacaa tcgatggagg
     4741 gggctttgct gccattgcgg ctacgattat cagctttttt ggagcaatct ttttggggca
     4801 agaaagtggc tttttccatc cagtccccct tgtactgttc gtaatttcag tgattgcctt
     4861 tgtcagcttt ttaaaagttg aaaagaaagc agcctctcca ttgatttctt tcggtatttt
     4921 taagaacaag atttttacca tgagcttgat tacagccttg ttgatttttt cttccaactt
     4981 ctttgtcaat gtagtgattc ctttttatct gcaaaatgct cgcgggttga gtcctagcta
     5041 tgctgggctg ttgatgatgg tctttccttt gatgatggtc gtcggggcgc ctttaagcgg
     5101 ctacctgacc gaccgggttg gcccggagat tttggtatta gtaggcttgg gcttattgtc
     5161 ggtaacccag gtcatgtata tgtttttgtc agtggactct cctttgtggc tatacattgt
     5221 cgctactgcc atcatgggtg ccggcaatgc cttgttccag tcccccaata atacgatggt
     5281 tatgagctcg gtcactatcg aaaacttggg actggcaggc tcgatgaatt cctttgcccg
     5341 aaatttgggg atggtcagcg ggatttcgct ttctacgacg gtcttatatc aagcgatgtc
     5401 tgctaagatg ggccagcaag taactaacta tatcgcaaat cggccggatg tctttttata
     5461 tggaatgaag attacctttt taggcagctt tgttttgtgt tttgttgcct ttttcctgac
     5521 tatctggcgt atcaaaaaac aaggaggtat tcagaaaaat gtctgaaaaa accactgttg
     5581 aagaattttt ggaggcctgc gtatgggtga gtgggatttc catggatgat ggaagatgaa
     5641 gtgaacttct aaaagccgaa aaactcaagt attatcttca aaatactagt gctcaatgag
     5701 atgcttgatt tgatcacaag aagactgcga aagccgctgg caaccgcaag ctaggtatcc
     5761 caccgatgga ctacgtttta gaggctactg gttatgaata cggagccaac gctctcatct
     5821 gcataagaat acatcaccca gattatgtaa agattttcga tacttctgtc aaaaaatcat
     5881 taagatagat aaatgatttc cgtctagtca aattggttca gaatgtagac acacagatgg
     5941 taagaagtca ctcacaattc gagaatggac ttgtcctatt tgccgtatac atcataatcg
     6001 aaacgtcaac gatagtatca atattttgac caagggctat gaatacaagc attgccttag
     6061 aaaacatcaa gaaccttagg aacaacgggg atagcttggt taataagaga taccgctgtt
     6121 taggcttgca agtatactct tttatcaaga attccgtggc tttagtcatg agaggcttaa
     6181 tactatgaaa attttcttta gcatttttga tttaggttcc ttttctaatt ataataagat
     6241 gaccactaca gtaacctact gaaatatata aacagccact gtagtaccat tttcaaagta
     6301 gtctatgaga ggagaatctt cattcttatg aacgctggaa ttgcggatat gaatttaata
     6361 aaaaaaaccc ttaatgattt tacttccaat tcaatatcaa aaggtactgg tatcaattta
     6421 agcacaataa aaaagctgaa aagtggtgaa agatcagtgg aaaagctgaa tcttttagat
     6481 gctataaaaa taacggaatt tgctatgaaa aatggaaaag ctgagattga aatatggaga
     6541 taattatcgt ctagtgaatt acttgccact tagctgaatt taacgatcct tggtgcttga
     6601 agggggagat tcttgggaat agagcatact tgcaagccta tgtctttact aacaacgata
     6661 tctcttatta accaagctat ccccgtagtt ctaaggcttt tacttttatc taagcaataa
     6721 tcgtattcaa aggaaatttt tgacccttag aaagtctcat tttatcagac gtgagcttag
     6781 gcattgctga attttgttaa ttcggtttta ggttgtgctt tgagcataat atgaaagtat
     6841 tctttatcac gatttcattc atctaaggta atgtgatgtg attctgaaat tctttcaaag
     6901 gcagtttttg cataagctga cattacatca tcaatgacat gtttacaatg tttcactact
     6961 aaaataaggg ggtaaccaag aagaaaaact gaatgattat ttgtatctaa ttccatggta
     7021 tatcaaccat ttcctatata gactgattat gacatggatt aaaataaaaa ggcactatgt
     7081 cttaattgtc ggttttcgag ttaccgaatg gcattcatca cccacctata gaggatgggc
     7141 gacttctgcc tgaaataagt tacactactt ataacttcta ataaaattag attgagtcat
     7201 tattatttaa catcaacgat tttccagttt ccattttttt gtaatactaa tttgtactga
     7261 aagtagctag tggtttttga tacattatca aggtatttta tctttgtact cactagaaaa
     7321 ttttttccgt tttgtagata aactggattc tctaactccg aaaactttaa gtcaatattg
     7381 attggtcgca tcgcaccatt ttcaacataa tactccaatt ctttttcaga agctgatgga
     7441 taaagtttaa aaaatgtttc taaaaaatcg gtgatttctt ccgttgtttt ggcatcaatt
     7501 tctgaatcgt tttctacttg ttttgcttca tattttgcct tgtctggctt tccaatcatt
     7561 gttggcaaac tagttactaa atgattcctg ttttcatctt caaagattgt cactcgatat
     7621 gcagaagaaa ttgattgcgt cttttttccc tctgtaatat gttgctccac taaaaatgaa
     7681 acgacaaatt cttccgagtg tttttcaaca tctagaattt ttacagattg aacttcggaa
     7741 ctcgtgggaa tatctgctcg aaccatatct tggattaaag cgagaccgtt ttctgtaagg
     7801 tactgttcga gattactcat tctcttttca attgcttctt tgttattttt ccatgaaaaa
     7861 tattctttcg caaaatcctc tgtaaaactt tcaacgccag aagtattcac taccttctct
     7921 tttataacca ctttttcatg agttgtatgt tgatcaatgg cggtgaaatt tttataaacg
     7981 ccaaaagcaa gactacaaga caataaaagc caaagggcaa gcaccatttt tcgatgctta
     8041 cccacggata caactcgttc ttttttcttt tttgggctct tttccttttg attccgttca
     8101 atttttatct tcattctttt tcctcttttc tcttatttgt atcggccagc tccgataaga
     8161 tgctgccgcc aatagttttc cgttagattc gtccacccca ctggatctcc tgcatgaaac
     8221 atgcgattct cccctgcgta aatgcccaca tgggtcacat aagtaccggc atcataggtt
     8281 gaatgaaaga aaactaaatc acctggtttg gcatctttta gatcaatatg agtcatggca
     8341 tcatattgat cctgagccac ccgaggaagt ttcaggtcaa ctttggcata acaccattgg
     8401 gtcaagccac tacaatcaaa ccccgtttct ggacttgagc caccaaacac ataggccgtt
     8461 ccttcatact taaatgcctc atcaaaaatt tcttggacta ttttgtcatc aaactgagtt
     8521 gtagttaggt attgcttcac taataaacag taaaacatat tgccataatt ataccgccac
     8581 ccaccatttt ccttaatcgc gattgggttg gggtaatcga ctttttgacc acctgatttt
     8641 tcctttgaaa aagattcagc taaagcaaaa ctatgcttct tcccgttttt cccgacatga
     8701 tctagatacc cgccaccata gttataggcc tgaataatac tatcttgatc acaccctagt
     8761 tctttggaac gcttaaccaa agacgcaaaa tagagacagc cttgtttgat ggatgcctcg
     8821 gtacttaatg aattaggtgg taatccggct gactcagaac tttgcataac gtcttccatt
     8881 gtgccaccag attccacttg aataatcgcc aaaagaattg gaatatactc tgttatatcc
     8941 gattctttgg catacttttc caccattgca cggtgtttca atacttcttc tgagacacta
     9001 gttccgccag gagaagtgcc agaactacca tttccctcac tgtcttcacc aaacaacaaa
     9061 cccacacaca tcaaaatacc cataaaagag atggtcagaa aagttaatga aataacaccc
     9121 actttcttca agttcatttt ttctcacctg accttgattt tggacgatgg ttcattcttt
     9181 tttgtacttt ttgattggtc tgctttggca gagtggacct ctgtttcact tgttggaaag
     9241 aatttttctt acttgctgat tggcttttcc cttttgaaat gatttcttga ctagttagtt
     9301 ttcgtttgat ttcgggattc agatttgctc tcacaatccg ctttggtgct tcttttttca
     9361 gcatcatatc tttctgaggt tgagctgctt tatctattgg aatattcgcc cttctttttc
     9421 cctgacgatt tgttgcttca ctcagtatct tgcgtttttc gtccattttc gcatttcttc
     9481 gttgtcgttg tttttctcgc aaatccactc gatccgcttt ttcttggaac agtccgcgct
     9541 tgaattcttc tggtgctttt ttcgactttt cgattccttt atggagattg tatttcaaat
     9601 tcgttggcgt attccttacc tgatccttta cttgtttagt cttatctttc accaaagctt
     9661 gggtatccaa aactttgccg atttttctcc cagccaaatt cattcgggat tgtttcttat
     9721 tatttgaaac tggattctcc ttagttaagt cagatagtat ttcgtgatcg ttcggtaatc
     9781 gtgaattctt tcgtagagaa gaaccggatg gcttaaattt ggattttgat tgttcctttg
     9841 ccaacttatt tccgactgct gcaccaccac caaaagccaa agctcgtccc acatttcgat
     9901 tgagttgccg caattttcga ttcattaaca tttgcggttt tcgcatgacg cgtcttccca
     9961 ttgactggct atcattactc tggaggttga acatactcat aatgtcgccc aatttgaaat
    10021 agataccggc aaaggtcaca atttgcagaa aagcaaccat gaaaaatgga taggttgccg
    10081 aaatattgaa aaacatcgtg gagatgctaa atgcagtcgt aatcaccaaa gtaacacccg
    10141 ctcgcatcat aatcgtattg aataaacgaa taatcgcctt ctttcctaaa ctttcataag
    10201 tcggcaacat ggacaataag aaactaattg gcagaaacat cgcaaagata atgaagagaa
    10261 tttgcgagaa caacataatc cctgttagaa gaaaaacaaa gaaggaaatg ccaatattga
    10321 acaataccaa aaagaccacg gtccctaaac ggttcatggt cttggtaata ctgagattcg
    10381 cattgtcatt gtcttcaatc tctgccttga ccgcttcttc ccgatctttg cctttgtttt
    10441 catctggact cacagataga atcttgttga cccggtcttc accaatttct tctatgttgg
    10501 aatcatcaaa ttgcagtagt aaccaaggtt gttggacttg aatcgaaaac aaactatccc
    10561 gaatcaaatc cacactatct tttccttggc tttctgaatt gggtacaacg atctcggttc
    10621 caagggtaag ggccgcttca ctaacatctg agctaaagtc attgattttt gtgatatagg
    10681 taggcgcata agcaatgaac gaacccgata gtagaaaaat catcacaaaa ttaatcacgg
    10741 ctcgaactgc tttcgtggtt tctcgtttta ataatccggt atacgccaca tacaccccca
    10801 gagctaaaat catcaataat agaaacccaa aataaaatcc ggaggtctgg agtccatttt
    10861 cagtaatacc agccaatgtt tggatattct ttccaatact ttcagccgta tccgaaatga
    10921 aatctaattt gtaagcctct tgaaccacat aacccgtggc gttcgacaaa tacaaactca
    10981 ccgtccaaat gaaattagta atggcataaa gaccatacat cacgcttttc cctaagccat
    11041 caccccaatt ccaaggcaac caatcccaag agctatccac aaagaaatct agttgatagt
    11101 tgttcagtga atatttggaa tatagatttc ctgcttcaac tgtatcatca actagccctg
    11161 tcgcctcggc taccgtacca actaaactca atagaactat cagagtgagt atgctaataa
    11221 aaacgattcc cacaatccga aaaagtttct ttttcatccc ctcactccat tctctcttga
    11281 acaggtggac gggtatcaaa ggcatggaag agttcttcaa agactggatg tacctgaacc
    11341 acgcctaccc gaccatacaa atcctgcatc aagcattggc cattttccaa atctcgtagg
    11401 cgtttttgat tgctttcatc ttctcgatcc aagccaaaga attccaaggt tttcttgatt
    11461 tcatgaatat cggtactgcg aaacgcaaac ttcaacccga tattattctt caagcgttca
    11521 tccagtaaat catcactatt ttgcgtgaca aagtaaaccc cggcattcat agcccgccca
    11581 gcacgaacga gcttattcga taatgttttc ccttgcgcta cttgaagaaa actccacgcc
    11641 tcatctaagt ctacaatttt gaagatttct cgattactat ggataaagtc caaggcaaaa
    11701 gtggaaatga cgattagcat ggaaacacta agcaattcca tggtggtata ttcttcaaaa
    11761 gtggtttctg catctggtaa aaccaaatcc gctacttgaa tgatatttaa ctgcctatct
    11821 aaactaatcg actgggtaat cgaaccatct gagaaaagca aatgggcaaa atcataatcg
    11881 gtaaaggact cgatgtgatc cgcgatattt tccgcaactg gtgagccttc ttttcgaagc
    11941 tcattaatta cttgtaataa tccccgttga ttgctttggg taaccgtccg aatggcctta
    12001 cgcaacactg gaaacttctc cccatctcga ctagaaattc ctgttaaaaa ggtcagaata
    12061 tcaattgcta gactttcaga atccttcttt ttctccataa tcacataagg atcaagtaat
    12121 ccttgatttt ttggcttact agttaagttg agaatattga tttcatccgc aatttctggc
    12181 aaggtttctt tccagccacc ccgttcccct tttggatcga caatcacagc ttgtccacca
    12241 aaaaggacgg cgtaataaac taagagattg ttactaaagg actttccgcc accgagtgaa
    12301 ccgaggaatg ccgctgctag cgcattggta actgaatcct tgaccccttg tgaggccaat
    12361 gccggtttca ggtaaacatt gcgccccgta tctacgttat aaccaaagta aatgccttcc
    12421 aactccccta acatctgagt ggcgccaaaa ccaagtccgg cgagaaaatc agacgtaaca
    12481 tactggatat aatcattcat gtatcgctta ctagaaggta agaattcttc atgtagcccc
    12541 atcatatcgc caaaaggccg gactagtttt acattggtat catcataaaa atctagcact
    12601 tcatcacacc ggcgctttag ttcatctact gattcggcac ttactcgaac cacatagctc
    12661 agtttgtaca tggattcttt ggactgatcc aaggtagttt ccaattcatc tacggaatcc
    12721 aatgcatcta agacattgga atttgtttca ttgtcagatt gataggcatg gttgtccaag
    12781 tcttttaatt cctttttctt attgcgaacg gtagcaagtg cctttttgtt cgtcacaatc
    12841 tccacattca tactagtgtc aattgggaat gtaaattgtt gttgctggta atagaaaagt
    12901 tctgaggagg gaaactccat ttcccccaca attgtattaa ttgttaagta cgctacatat
    12961 gattcatgat tctcatgttc catgcataag taccggggct tttcttcaat caaacaacga
    13021 ctgggacgta ataagtcgta acgtttcacc aaagtttccg acttcagctt tttctttggt
    13081 agctgaaatt catattcttc aaatggtgta cccttttcgc catagatatg ttcaatcaaa
    13141 tacatcagat cactcggtgt gactcgtctg actttaaatc ttcgagccaa tttactttcc
    13201 attagttttt caagcttggt atatcggcga atttcttcat tggataagga aacaaaatcg
    13261 cccatcaaat gatgattcac tccatagaca aattcttgaa agccggagaa gaaagatttc
    13321 ttgagattct ttagattgac ctcttcatct gtcgcaatca gtttgaagcc aataaaaaag
    13381 cgataatcaa tctgactatc gccaatcatg gatactaatg cctctgtctg taaatcaatt
    13441 cgttgtttcg ctacttctgt taatcgcccg gtaatttcct tttttgatcg ttcttgcgtg
    13501 gcacgaatac tactttctgt ggcaatttgt aaggcatgaa tcttgccttc acgattttgc
    13561 gcaatcaatt gacgaaagtt atcgtggact tgatatttct gttcaggtga caaaaaggag
    13621 taattgtaag gtattaactc atagtaggca aaacattctc cctcttgatt aaagacaaga
    13681 ttattttcaa tatacttaat cgggtacata gttcacactc cttaccagag taatgggttc
    13741 gtcccatcgt ttcttctgtt ctttgacttt ctttcctgcg aaagtgacct taaatcgtaa
    13801 gtgatagcta ataaaagatc gtaaaaatcc caatggtttc ttaccgtcaa acgttttttg
    13861 cgacatgaac caagtaactg ccaccggtat acctaagtat tttaaaaagg ccccctctat
    13921 catagatagt ggaggcacat gggcaaatac gatgacaaac agcaaggata aaacaaacca
    13981 agccatctgg ttaaaagtca caggaaaagg cagttgaaag tcattaattg cataaatgac
    14041 cttttcaact gcccaaatac tggtgtagct ttttattttc ttcatcaaat cattccttta
    14101 aaaagaagga tagtttagaa atcaactacc cttcaataat tttgttaata tggatactca
    14161 aaaacacctc gactcgttac caaataattg cctccaatct ccaagtctct accacacgca
    14221 tcaaaatcaa tatacgtctt caagtgttct ggtacttctc caaaatcacc agcttctaca
    14281 atcaagaaat atgctacatc actcatatca tcgcaatctg gataatagat aatatcatcc
    14341 acatgctcta ccatctcctc aaaagaattg aagaaagcat gttgaatctc agaagcaact
    14401 tcaccaactg gcgttccttc aagttcctcc gctaagccac acaaccgatt aatttcctca
    14461 attggcgtat actcatcaat ctcaaaggga agttcataat catgaattgc atattcctcg
    14521 tattcgtcat ttaacccaat tcgttctttc acttcatcaa aatcgacagg tggggtaaac
    14581 cacgccccca ccaattcacc ttcgttatat ttcccgaggt tcgcaatata aacacgcatc
    14641 tgttccatcg tgcttccccc ttacgcaccg acaattcggt taaacaaact gagtaagaca
    14701 tccttcaccc cagaagcatt aaagaccaat ccgaccgcaa tcaaagcaat caccaaaaag
    14761 ccaatcagtt tggaaaactc ccgcttaaat cctaaaaaca aaccaatgac cacaattgcc
    14821 agtaagacta aggattgggc attggttaaa aaccattgat atatggatag gtaagttcct
    14881 gatattataa atatctcaaa cttttccccc cattcacacg gagcgtgcga ctttcaccgc
    14941 actccgcgtt ccatcgtgtt tatattgaaa agtatttatg tagtagacct atatcaagtt
    15001 taattcttga ttgccagcta atactctata tttatttagt cttatgagct ggactttatt
    15061 tagcttcaat agagctatat atttttgtat tgtttcttct gttttagcgt gtattagacg
    15121 gtgtaccgcc tcttttacca aaataagatt tttataatcg tcatttccac cagctccagt
    15181 tggtacaata tgatgacaat gtatttcatt agcttgttga aattcttcca gagttatagc
    15241 acattttcca tgttgagcag aaaatagcga aattcgattg tctgcgtatt ctattgtgcg
    15301 attttccaaa gataatttca ttaactggta catcagtaat ttatcaacac ttagattggt
    15361 gtggataaat tttcttccct ctagagtgta gcaattcgct aaatacacct ttgccattgg
    15421 ttttctgtgt tggatatatc caacagggaa aataggttcg ccctcgccag caacataccg
    15481 aagcatttta gatttaccat atcttgttgc ctcctttgtc gtaagttgtc tgcctgtttt
    15541 tctcaatctg ccccttttgt ttgtacctaa tctgttagtg aagatagcca ttacagctct
    15601 attcagtttc atacaatcca gatttacatt cgttgcaatc tgataataat tttgcatacc
    15661 taataccatt gaattataaa gtcttgtttc ttctaattcc gttttccctt ttgagggaga
    15721 agcaattttc tttgcctgtg ataccagctt gtctttttgg tttttgaatt gtttatcact
    15781 tatatgtgat ttaacaacta acttctttct tctcggtatc attttaatct tgaaaccaag
    15841 aaagtcggag tatctttttc taacatttac aattcttgtt ttttcttgtg atacttccaa
    15901 ttttaatcgt tgttcaatcc attgagtgat tgctattttg gctttttcag cactttcctt
    15961 gtagcggcag aaaactctaa aatcatctgc ataacgaacc atatacattt cctttaactt
    16021 tgttttcttc atttctttgt aagcgttgct tttacatggg ctaccgcttt tattgatatg
    16081 aaccacatag ttttttgtaa caggattttc ctgccattgg ctttctaccc aatggtctaa
    16141 ctcattaaga acaatgtttg ctagtagtgg ggatattatt cccccttgcg gagttccttt
    16201 ctctggatat gttattgtac cgttagtcat tttgattgga gcttttaata tcctttttaa
    16261 aatgaatatc aagtgcttgt cccgtattcc catagcccat atctgtttta tcagcttgga
    16321 atggttcacg ttgtcaaaga aacccttaat atcaaattca atcacataat gtagctggct
    16381 gatttgtagg cggttatagg ttgcttttat tgcattttca actgacctat taggtctaaa
    16441 accatagctg ttttcactaa atttagcttc gcagattggt tctaatactt gttttataca
    16501 ttgttgtata agcctgtccc acatacaagg aatacctaga ggtctggttt taccatttgg
    16561 ttttggtatt tcttttcgcc tcactggctt aggtcgatac ccatgtggac tgcctgcaac
    16621 aatgtatctt acttttttga caactgtttc tgctggcaat ttgccaatat ctttaattat
    16681 tgtaccgtct gttcctgctg ttttactgcc tgcatttgct ttgatatttc gatatgccag
    16741 taatatattt tctctcgata ggataatatc cataagatga gtgaaaatat ctccattctt
    16801 acttttggca tataaatcat caaaggtgtt ttgcatttcg taatattcag cgtgtcttaa
    16861 atcttctaca cacaatgttt catttttatt tttaggcata aggcttcact cctttacttg
    16921 aaattgagtg agctttttta tcttactcgt aatccttatt gaaacaacat aatacttttc
    16981 taatatatac acgacttgag ggcattcctc cattctcatt acaagaattt cttaggttcg
    17041 ccctctgctt ttcagcatta gttcaactgc ttattgttct tcgtcagttt attctcttga
    17101 tgagaaccca atacctttcc tcgttccgat aatcttatct atacatatat atccttaggt
    17161 cactagctat aagcctgctg gcttgatacc gcctgtaacg ttatatggca tttcataacc
    17221 tatattctta ctttgccaca ccagctacga tttctcgtac ctttgcattt ctacaaagtt
    17281 caaatttaga cccgtacatt cgctgtttcg tcagttcctt atagaacatt ctaaccatag
    17341 atattttata gacccccgac ctgttggata cgctacccac ctctggatag ttttcgatag
    17401 aagatatatg ttcatcaact attacggtat ctttcagccg tctttaccga gcttcaaaca
    17461 ttctgagttt cctcatacaa tgcttgtcgg aggctcaggg caggcgtttc agagcgttac
    17521 ttcatcattc cacctgtcag attatcagtt ctcctaataa ccaaactttt tcgttctttt
    17581 attagtgcct tagcttttca ctaaggaacg agtcgcacat aaattttgtc cgaaattcat
    17641 cttgtcactt tcctttcttt tccattgctc gatccgctgc tgcacttgca ttcatcaagc
    17701 acatcacagt aacaccaagc atactgccac caaataccaa aaaaatatct tttacaattc
    17761 ccatgacgat tcctcttttc atataatcaa attttcaatt gccatacttt gttgctccaa
    17821 aatcttttta tgtcggtctg ataagcgtgc attctgtacc atatcctgga tataggttgt
    17881 ccgattcaat cgatccaact ccttagccat tttccacgta ggggcgactt gtcggcctag
    17941 ccaattcaag gttcgttcaa tggtatacgg ttttggctcg gaagttaatt gaatctcttt
    18001 ccgattccgt ccaataaaac gcccccattg ttcattcaag ggccagttgg ttcgcctttt
    18061 accttctact ttatccgcaa atcgcaagta acgattgata atggaaaacg tggtgctctc
    18121 aatgctacga ccttttaaca aatcttgaat cgcatgaaaa gcacggtcat ttttcagtcg
    18181 aatctcaaaa cgatttttcg tctcagtttg gtctaatgga atccctaact tgatatattg
    18241 ttcatagtct ttttcataca aacagaaata aacctcactc ttgagactgc cgatatataa
    18301 ggtattgccc atgccgtcct tttcatcagc ctttaaaagt tccccggaac gataactttt
    18361 aaaggtacga aacaaggaga tacattcttc cttctgacat tttttcgtta gctcaggtat
    18421 atccaatagc cccactcgat cattgattgc caagtccaag cgtttcatca cgccaccggc
    18481 ctttagacaa tcctcgaaaa agtcgtacca gctacgcttt tgagcgagta aaaaagtttc
    18541 aaattgtcgg caacctcgcc cctttaattc taaaagcaca cctttatctt cttcatgaga
    18601 caacatcacc acaatatcac ccatgacata ctgttcttga taggagtaaa aggcataatc
    18661 ttcatggagc atatactcaa atttcaaatg gagaatttct tgaataattt ttcgtgcatc
    18721 cgttgtggga aagcgaatcc ttacatagtc aaacagtaac gtcaaaggca aatcgggatt
    18781 aaagctttcc agttggtcaa aaattttcct catggttttt atagatgcgg ttacgccacc
    18841 attttctatc tgatttaagt acggccgact aagtccacaa gccgtagcca atttattttg
    18901 tgaaacgccg tattcgttgc gtcggtcttt taatagccga taatctaaat tccgttgtgc
    18961 caagttctca cctccaaatt caaggtgtta actttttcaa atgagttaac acctatacaa
    19021 attacttact atctttgata caacaagcat tcttaatggt tgcttactta ttttttgtgt
    19081 ttttgtaccc ccctgttaga tacgggggtg agacatcatg gtcggctaac gccgtccatg
    19141 aacaggcaca gcaccacgga ggatttctgc ttcgcgaatc ctccgcggtc agcgcctaac
    19201 agtcagttgt gttggtttag gttactcatc tgtaatacca tttacttcca actctgcttt
    19261 catttctaac aagtattgac gaatatctgg ttcttttgct tggtccgcaa tccgctcaaa
    19321 ttctgcaatt acttgattta tttcaattcg agcatccgtc tcctcattca ttaaatatgg
    19381 atcgccagcg actttgacac cattcttgcg aatatcaaat tttaaccgtc gcaattgttc
    19441 ttctaattct gggtctgact gaatctttcc aatcacccaa taaatttctc caatcaactc
    19501 atcataaacc atgtacatcc aactctttta atcttctggc aatatcaaaa atgaccggaa
    19561 ctgttacaga gtttccggct tgcttataca actgactatc tgaattgact tgagaatctc
    19621 gatcaaaagc ccaatcggga aagccctgaa gtcgccaaca ctctctaggt gttagcttcc
    19681 gaatttgata ctgatccgtg acgatcgctt gttggtctcc tgttagtaag gtatgaacac
    19741 tctgttttcc cagacgacct cggcgacttg tggaattagg aaaagccaga ttcacactgt
    19801 cttctggctt cacctcttga tattctgttt cattttttcg aatcatgatt tttgtaccac
    19861 cttgtttcgt tgacaaggtg ggggcggttc cagtaacttc ataaactcga ttaccttgtt
    19921 caaactttcc cggtaatagt cctgctacag agattccatc ccttgctggc agtgcgattt
    19981 tgattccttc atcacctgtt aaggttggcg ctacgccatc tgaaccatag acttgtccac
    20041 ccattcctcg attggaagga ttgatattta ccaaatttct gatgttttga tcagatgttg
    20101 aagacggtcc aatgatagga aatacttgtt cggtacatgc gtctcgagaa tgtccgataa
    20161 ggaatatccg ctcccggctt tggggaatgt aggcctttga gttaagcacc taccactcca
    20221 cgtcataccc caatccatcc agcgttcgga ggatcgtctc gaacgtagcc cctccttcgt
    20281 gattgagcaa tcctcggacg ttctcaagga ataaaagctt aggtcggaga agagtggtga
    20341 accgagcgat ttcaaagaat aaagtacctc gagtatcgga aaatctttgc cgctttcccg
    20401 caattgaaaa agcttggcac ggaaatccgc cgcaaaggat gtccactggt ccgagggatt
    20461 gaataaactc atctgataca cttgtgatgt catgcatttc cacctcctta cttgtatcat
    20521 gaattgcttt ataactacgt ctagcaaatt tatcaatttc acaaaaaccg atacaatgat
    20581 ggctcgcctg ttccatacct aaacgaaagc caccaatacc ggcgaataaa tctaaaaatc
    20641 tcatacatta cctcattcat tgcaaagaaa aaagccaaga acattttgct cttggctcat
    20701 acattattaa tttgtcctac tattttttat ttaatccaac taatttccgt tgtttttcat
    20761 tggcctgatc ggctacatat ttaatcaccg ttttgaactc ttttgcagta gcattttccg
    20821 ataaatccgc aatgctctta ttgtatttct ttacaatttt cgttacttgt tctttctcag
    20881 tcatgtaatt gaacctccaa atctttccta ctatacccta gtggtaaaaa taattctaga
    20941 ttatccgtag cataatgaac ttcggaaata ttattcgtat aatcttttat tatgatatga
    21001 agcaatacat ctggatgttt actctttact tttgcaatgt tctctcttga tttgaaaaaa
    21061 gcgttaataa atgccttttt cggcacatga cgtccctctg caatttcacg ttcttgtgta
    21121 aatttccaag cgattttagg gtcttgataa acatagaaaa tcgtcactcg aaaatgtttt
    21181 ttcaacgccc gcgagatatt tttttcagca cttaatattg caaaagttgc atccagaata
    21241 aaagaatagc catgatctaa gacatatcta aatgcctgct ctaccaacca agaagatgct
    21301 ttttgaaaga gactagagtt ttttccatta taatcaggaa actgggtgcg aaagtaatcc
    21361 gcatcaataa tcacatggtt atcataattc tcagctaatc caatggcaac ctctgatttt
    21421 ccagccccag gactccctgc cataaaaact gcgtctcgtc caacttcggg ggatttccct
    21481 ttgataatat aagaaataaa cttatcctta tttgcttttg catactctaa ataagaatca
    21541 ttactttcca tattaattat cccctttctt cctactatat aaaagtatta tacgttactt
    21601 gaagtataaa gtcagtattg aagatcactc aatcattcat cttttctaat ttactaattg
    21661 aatctaagaa atcataccct tttggtacaa gtggcgtata aaactcacta atcacactgc
    21721 cacctgtatc cacataacct cgacctttga tgtgcttcat aaagaagttt ttttcgacct
    21781 ccccaaacat catggaatat ccgagttcac tcatacgacc caaagccacg cgaaagttga
    21841 attgatcgcg gattccgtct cctaaatatt tcgcatccgg cctttggcaa gccaaaatca
    21901 gaaagaaacc agattgacga cccaacatca cgatttgttt gagcttattc aaaatcaccg
    21961 cactttcttt cgtcgttaac atctccatat aagccacgta ttcatcaaag attaaaaagt
    22021 tgggtggaag tccaagatag gcataattct ctcctgtttt gtagttagac atttctttca
    22081 ttgccttgct acgagccatc atacgttcat agaaatcttc cacacaagca gaaatttctt
    22141 ccttttgtga ataaacatga ggcatcaccg tacctaaatc agctaaatct gcatttttgg
    22201 gatcgaggat aaacaattct gcatctgact tcaatagtgc ttcgataatg gtgaggagga
    22261 aataggtctt ccctccaccc gtgccaccag cgattaacat atggggtaaa gaatcataag
    22321 cccatacttg atttttcatc aatcgcagag tcccattttc tgccactact tcgtctatcc
    22381 caattcgatt ggcgatcata tcatacagta aagtatactc cacataggag tctttgagtt
    22441 ccttttctac taactcacaa tacagtccgc tttctaactt cttttccaat tttaaaagct
    22501 gatcttggta tttcccaagt gaaatttgaa cgcgaataga cagtaatcca tctttcattc
    22561 gataacagat tttggggaag taactgatag tctctctggt tcggctacta ttcaaatctt
    22621 tgaaaaagcc ttcactttga gtttgtttca cttcatacca atggttctct agaatcatcc
    22681 gagctagctt ttgtcgatgc cacaagcttc gccaatgatc aagaaaaaag tgaatataaa
    22741 gcaatatcat agctcctgaa aaagcgagac ttccaagcag acatactaaa tcttttatag
    22801 accaaacgat ttcaatagta gaaagtgata gcttagaaat atcaaattgt ctaaactctt
    22861 gaaaataaaa gacgctcatc caaccgatga acagagcgag tagccagcca agaacaaagt
    22921 gataaaccaa gtgctgatct cctgcgcgta tgcgatgtcc tttgtacatt ttgatggttt
    22981 ccacctccaa tcgaaaaagc ccaataggat ttcattaggc aatttcaaaa agttatgcta
    23041 gtcatttact gctacgagga ggggagttgt ttcgtttcaa ccaattgatt taaaaagcct
    23101 tcaatagcat ccggcatctc cacaccaatt tcttccaaaa tggattctgc ctgccgacat
    23161 aagttttcat ttacttcgac aactataaag aaagactctt cttctcctaa ccagtctagt
    23221 acatcagagt cttcttcact gtcgctattt tctccaagta aaccttgatg tataatcaga
    23281 aacatttttt catcagaggt taatgcttta acgagttgtt cctcagtcag ctcgcctttt
    23341 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccataat cttcacctat
    23401 ttctttcttt cattttggtt cgtaggattt ccttgattcg gctgccgctt caagactaaa
    23461 tcatccgcct taatgaacca attaacggta gttccttctc gaaaaacata attggcaacg
    23521 gtatccacga ttggattgac caactctacc tctgcatcat aagcaaactc gcgtaagggt
    23581 acattagctg ggatactaac ttgaatcatt cgcccttgtg cagaggattt taaattatag
    23641 gttcgttctt ttacctcatt accaattgtt ccatcctcat tttgcttccg tacttctcgg
    23701 cgtaatgcgg aaaaacgcaa cttcccaaat gttaaacctg aatctacgac aataccttct
    23761 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcaacata caacacgtaa
    23821 tcagtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
    23881 tttagcggtt ggtcttgatc aaaatccttt tcaccggctt ccgcagctac taccacctca
    23941 atatcatcgg cccgttgctt actggaatac aaatgataac tgcgaccaat caccgtagta
    24001 ttccgtccat atccttctgt tataatttct ccttcaccgg cataacttaa ctttccaaag
    24061 gtttcagcca tatctggcac gacaaatttc aattccatct tcttttcctc ttttcattgt
    24121 tagttttgga tacaaaaata gcgcgactac ttttttagga ggcggtcgcg cacgttggtt
    24181 cttaggattt acttgtatca tctcatcacc ttagttccgg cgattccaga aataatagcc
    24241 cgccgtcgca aaaatcaaag taaaaccaat gaacagtaaa acattggaat ttttctcccc
    24301 ggtttgaggg aaggtcttca ctgtgggact agattcttga gagccacttg gaacagttgg
    24361 tgtttccggt tgtttcggcg tacttagtat ggttggaact tcttttggtg ttaaggtttg
    24421 agatttttct ttcaaatctt cattgtgttt tccattgata ttcccatctt tatcgtaatt
    24481 gatttccgca aaagtaaact tggttccttc tggatatttt ccggtatcta cttttttcgc
    24541 aagcacagtt ttggtgaatt ctttatcatt tacttcataa tcaatcttgc cagatttcca
    24601 aatttctttg tttgtaccat ccggtaataa agcatacaga attgtttcga aagcttcttt
    24661 tgagccatcc agtacatcat gagtaatcga cacatcatca aacatatcca ccacatcacc
    24721 atgagtaaag gtctgagaac cgttttctag gtgggccttt gtttggatag aaacatggtg
    24781 ttccactgta caactgacag tttgggcttg gttattcaag ctaacatcct tcgctactgg
    24841 cttatcgcct gcttcaaaag cttccttatt ttcatacaca tagttgaata agacaatagt
    24901 tttatttaaa acttgttcgg cggttaattt atgctgaatt ttccaagttc ctgttttctc
    24961 tttgttcgcc aacgttgcgc ttgtttctgc aatcaccact ggtttggctt tttcatcttt
    25021 ttcttgggca gcttttgtcg catccacatc aatggcttgc gctaccacat accagtcttc
    25081 tttgatttca tgtagattgt aactcaattt atcaactagc tcggtagaat ccagagaagt
    25141 taattctttg tttccgtctt tccaagtcgc aagagaagtt aaactatctt cttcctcagg
    25201 caaatcatag agcattaaac ggttcaaact aacagaaaat gctttgttcg tcagcttctc
    25261 gtaaggaacg gtcaccgttt taattggttg tttttggtct tgttcggtaa tggtaaagac
    25321 atactcactc ttcacaaagt cttctttgtt ttccttaaaa gtagaacgga tttctaatgg
    25381 cgtaatcttt tgaaaacctt ctggggcttc tacttcttca agtaaataat ccccataagg
    25441 aagattttca aacttgccat aaccatcaaa ccctaattgc tcgttataag ctgtggtcgc
    25501 ttcatcttcg gcacctgtga tttcattggt cccttccaat ggcgacactt taaaggtcag
    25561 atcattgaag ccagtttcgg tagtgcccgc agctgatcca gcaaatttaa agaaatcaaa
    25621 gccaaagcga atcacttgtt cttttgccgt aacatttcgg gtaatcacgg catttttttc
    25681 gctatcatcc acctttttga tggatacagg atactttgtt tcatcaaggc tatatccttc
    25741 gggcgctttt gtttcttgcc aataatactc gttgatagct aaatgtttaa cggcaacttg
    25801 gttcttttcg tctaaagtca aagtgaccgt ttcatcagaa cctttcgttc ccttcgctaa
    25861 ctctggttta aaagcctcac tccatttaac agcttttcca tccttagcag taaacaaggt
    25921 gtattcagca ccctcaaaga ctgccttacc ttgtgcttta tccccagtat ctttgtcttc
    25981 tttcgtcaaa gtggtttcgc cagtgacttc ttgattttgc ccttttacgt tactggtaac
    26041 aggggctacg gtttgattgg catacttcaa ttcgactttt acaggtttga acgtattcac
    26101 gaacccatga ctagctttag tttcggtcac ataataggtt cccaattcca acgcattagc
    26161 aatctctttt ggtgtttccg catgaccgtt ttcatccgtg gtcatttctt gaacgatttc
    26221 accggttgga ctgtctttgc gaatggcaaa cgtattacca gctaaggaat aattgtcatt
    26281 ccaaagatca ctccctgttt caactccgct tttatccaga atgatttgcc ctttttcccg
    26341 tgcgtttttt gaagtcacgt aaatggtttc gcctgcttta atggtggtag tcatgggggt
    26401 ggtatcaatc gtataaggcg ccggtactga tttttcggta atggttactt ttgtaccatg
    26461 aggaatctca tccaatgtag caatgccttc tttgtcagta gtcacgtctt ttgcaggtaa
    26521 agtttttcca aagtctaaat ggaaaaccgt tcctggtaca acatcacccg attctttatc
    26581 gacttttttg atctttaaag aaccctccgt ttccacatca attttgattg tgtaggtatt
    26641 aggtttatca atcgccccgg ccattaaagt ctgttggcca gcctttttat aggcgacagg
    26701 tgttccggta ccagcagatt tcttaagcgt cagcacacca ctttcttttg aattggcatt
    26761 tggggtgatt atcaactgat taccattcac acgataatca atgtttgcgg cattttccac
    26821 aacctcatcg aactctgaca aattcagatt attcgtatcc gtcacagtag tcgattgccc
    26881 taatactgtt ttagccgtac tattatggaa ggttggtttc ttctgataat cgtcaatggc
    26941 ttgatttatt tttgcctcaa tggctgcgat atttacggca ctaccatctg atcgttttat
    27001 tgagtgaagg gtatacccat taacttcttg ccagatcatc ttctgtgcca caatatgagt
    27061 atctgcatct gtacctactt ttttccataa aacggaaact aatttggctt tatcggacat
    27121 atctggtaac gggtttttct catagccagg agtgaactca ttgtaaatcg ggactcctgg
    27181 ttcaatacag aaaatatctt gtttaaagcc gttgtacact gcataatgcg gaacagtgac
    27241 ttctgacatt ggacgaccat cactaaaagt tccttcaaca gcatattgat gtgctaaatc
    27301 tagttctacg gttaccgttt gtggatgggt aatttcatca gcaaaagtaa tcgtaggagc
    27361 aattgctcca acaattgctt gaacagccaa actcaaaata gttaatacac ataatttctt
    27421 tttttgtttt ttatacatga aatgttctcc tttcaaatta agcacaaaaa aataagatgc
    27481 tcgcttgaac atctttgttt ataaagtctt tttaaaattt ttattgactg aataga
//
LOCUS       DAZAID010000029        26923 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000029 DAZAID010000000
VERSION     DAZAID010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.029"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..1094
                     /locus_tag="ACG9BE_001171"
     CDS             90..1094
                     /locus_tag="ACG9BE_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3031928.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1095..2039
                     /locus_tag="ACG9BE_001172"
     CDS             1095..2039
                     /locus_tag="ACG9BE_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3031929.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2039..3001
                     /gene="opp4B"
                     /locus_tag="ACG9BE_001173"
     CDS             2039..3001
                     /gene="opp4B"
                     /locus_tag="ACG9BE_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFN3031930.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3028..3942
                     /locus_tag="ACG9BE_001174"
     CDS             3028..3942
                     /locus_tag="ACG9BE_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3031931.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3968..5749
                     /locus_tag="ACG9BE_001175"
     CDS             3968..5749
                     /locus_tag="ACG9BE_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3031932.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6097..6783
                     /gene="rnc"
                     /locus_tag="ACG9BE_001176"
     CDS             6097..6783
                     /gene="rnc"
                     /locus_tag="ACG9BE_001176"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFN3031933.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6803..10384
                     /gene="smc"
                     /locus_tag="ACG9BE_001177"
     CDS             6803..10384
                     /gene="smc"
                     /locus_tag="ACG9BE_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFN3031934.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10393..11202
                     /locus_tag="ACG9BE_001178"
     CDS             10393..11202
                     /locus_tag="ACG9BE_001178"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3031935.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11213..12211
                     /gene="ftsY"
                     /locus_tag="ACG9BE_001179"
     CDS             11213..12211
                     /gene="ftsY"
                     /locus_tag="ACG9BE_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFN3031936.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12398..14665
                     /gene="gshAB"
                     /locus_tag="ACG9BE_001180"
     CDS             12398..14665
                     /gene="gshAB"
                     /locus_tag="ACG9BE_001180"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFN3031937.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14754..15206)
                     /locus_tag="ACG9BE_001181"
     CDS             complement(14754..15206)
                     /locus_tag="ACG9BE_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFN3031938.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15356..16306
                     /locus_tag="ACG9BE_001182"
     CDS             15356..16306
                     /locus_tag="ACG9BE_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN3031939.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16299..17258
                     /locus_tag="ACG9BE_001183"
     CDS             16299..17258
                     /locus_tag="ACG9BE_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN3031940.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17255..18010
                     /locus_tag="ACG9BE_001184"
     CDS             17255..18010
                     /locus_tag="ACG9BE_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3031941.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18033..18986
                     /locus_tag="ACG9BE_001185"
     CDS             18033..18986
                     /locus_tag="ACG9BE_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3031942.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19630..19926)
                     /locus_tag="ACG9BE_001186"
     CDS             complement(19630..19926)
                     /locus_tag="ACG9BE_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFN3031943.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20281..20637
                     /locus_tag="ACG9BE_001187"
     CDS             20281..20637
                     /locus_tag="ACG9BE_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN3031944.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20647..21546
                     /locus_tag="ACG9BE_001188"
     CDS             20647..21546
                     /locus_tag="ACG9BE_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN3031945.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22066..22794
                     /locus_tag="ACG9BE_001189"
     CDS             22066..22794
                     /locus_tag="ACG9BE_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN3031946.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSRWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22939..24966
                     /locus_tag="ACG9BE_001190"
     CDS             22939..24966
                     /locus_tag="ACG9BE_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFN3031947.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            25074..26171
                     /locus_tag="ACG9BE_001191"
     CDS             25074..26171
                     /locus_tag="ACG9BE_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFN3031948.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            26478..>26923
                     /locus_tag="ACG9BE_001192"
     CDS             26478..>26923
                     /locus_tag="ACG9BE_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3031949.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT         9025 a         4663 c         5684 g         7551 t
ORIGIN      
        1 ataacctttg ttataaaacg ctttattctg tgtaatgtcc ttttttatta agaaaaactt
       61 ccatttagga agcagagagg ggtattcgat tgtccgataa ccaattatta gaagttcaga
      121 acctacatac aggattccga ctcaaagatg actattacga tgcagtagat gacgtttcat
      181 ttactttaga caaaaacgag attttagcaa tcgttgggga atctgggtgc gggaaaagta
      241 ccttggctac tacgattatg ggattacttg atcctaataa cacaaaaatt acaggtgaga
      301 ttttatataa cgatctgaat ttaatcgacc tgaacgaaac gctctataat aaaatccgcg
      361 gaaatgatat cggaatgatt ttccaagatc cgttgtctgc attaaatcct ctcatgagaa
      421 tcgaagacca gatcaaagaa ggactttcct atcacacaaa gatgaatgca gaacagcgtc
      481 aagctcgtgc actagaattg ctagaacaag tagggattcc taatcctgcg agagttggac
      541 gccaatatcc ccatgaacta tcaggcggta tgagacaacg tgtcatcatc gcaatcgcaa
      601 tcgcctgcaa acctccaatc gtcattgcag atgaaccgac gacagcccta gatgtaacca
      661 tccaagcaca gattcttgat ttactgaaag accttcagga agaaacaaaa acagggatca
      721 tcttgatcac tcatgactta ggagtcgtag cagaaatggc tgatcgagtg gcagtgatgt
      781 atggcggaca gttcgttgaa gtagcaggtg taaaagaact gtttgatcat cctaagcatc
      841 cttatacacg ctctttactg aactctatac cacaagaagg aaatcatgat gcagaactcc
      901 atgtgatcga aggtgtcgtt ccttctctga aaaacatgcc tcgtaaaggt tgtcggtttg
      961 caccacgtat tccatggata ccagcaagcg atcatgaaga agagcctatc cttcatgaag
     1021 tcgcaccgaa ccacttggta cgttgctcct gctataagca tttccacttt agagacgaaa
     1081 aaggagaggt gtagatgaca gaattaatca caatcaaaga cctgaaagtt cattacccta
     1141 ttcgcagtgg attcttcaat cgagtaacgg atcatgtata tgctgtagat ggtgtggatt
     1201 ttattatcga aaaaggaaag acatatggtt tagtgggaga gtctggctct ggaaaatcta
     1261 ctacgggaaa agccgttgtg ggattagaaa aggtaacagc cggacaaatc atgtatgaag
     1321 gaaaagatgt aacgaagcga agcaaccgca aaaaaatggg ttataacaag gatgtgcaga
     1381 tgatcttcca agattcaatg tccagtttga atcctaaaaa gcgagtgttg gatatcattg
     1441 ctgaacccat ccgaaatttt gaacggttaa gcgatcaaga agaaaagaaa aaggtaaaag
     1501 gactgctaga tatcgttggg atgccggaag atgcgctata taaatatcct catgaatttt
     1561 caggcggaca acgtcaacgt ctaggagtag cccgtgctgt tgctaccaat ccaaaactga
     1621 tcgtagccga tgaacctgta tctgctctgg atctatctgt tcaagcgcaa gtattgaatt
     1681 tcatgaaacg aatccagcaa gaatacggcc tcagctattt attcatttca catgatttag
     1741 gtgtagtaaa acacatgtgc gacaatatcg caattatgta caaaggtcga tttgtagaaa
     1801 tcggtacaag agaagacatt tataatgacc cgcgacatat ctatacgaaa cgtctgcttt
     1861 cagctattcc gcaaatcgat gtagaaaata gggaaagcca caaagaaaat cgacgaagag
     1921 tagaacaaga gtatatccaa aaccaaaaag aatactatga tgcgactggt cgagtctacg
     1981 atttgcgtac gatcacaact acacacaaag tggcgttgaa agatggaggt gcaagctaat
     2041 gtggaaaacg attctgcgac gtgttttatt gatgatcccg caagttatta ttttaagtgt
     2101 tctgatcttc ttattagccc aagcgatgcc gggggatcca tttaccggtt tgatcaatcc
     2161 aaatcaggat ccggcagtga tcgaacagat gcgacaagca gctggcttga atgacccatg
     2221 gtatgtccag tacttccgtt ggatcggaaa tgctttgcaa ggcgattttg gtcaaagttt
     2281 cttatacaaa cgctctgttg ctgacttgat cggcgaacgg atcatcaaca ctttatatct
     2341 atcgatcttg acagttgtcc ttacttattt gattgctgtg cctctgggga tgcttgctgg
     2401 ccggtatcaa aattcaatct tggataagat tgtcgtaatc tacaacttct ttagttttgc
     2461 tgttccttta tttatcttcg gcttgatcat gctatttgtt tttggttatc gtttaggctg
     2521 gttcccaaca agtggatcac aaacactagg tttgcaaggt ggatttatca ctcaatggat
     2581 agaccgtttg aaatatattc tgttaccatc aattacccaa gcatttttgg cgactgccgt
     2641 cacaatccaa tatctgcgaa gtgaagtaat cgattcgaaa tcgctcgatt ttgtcagaac
     2701 agcccgttcc aaaggggtac cgacgaataa agtattcagt cgacatattt tccgaaatgc
     2761 agcgttgccg atggcttctc aaatggggta tgaaatcact tcgttgattg ctggttctgt
     2821 agttatcgaa aagatttttg gttatccggg agtcggaaaa ctatttattg attccatcgg
     2881 ccaacgggac tataccgtga tcacatcctt agtattgatt ttaggaatcg cgacgctagt
     2941 ggggacatta ctttcagata tcatcatgag tatcgttgac ccgcgtattc ggattcaata
     3001 aaaccaatca gaaaggagaa tggatcgatg agtgaaaaaa acaatacagc agttcaagaa
     3061 agtattcctc ctatggggat acgaatgatt gctcgagaat ttaaaaaaga taaaattgct
     3121 attttttcat taggtttgct agtggtttta ttattagtga tctttatcgg tgcgatgctg
     3181 accgatcaag acaaagtgat gtatgttagt atcttcgaca aatacgctgc accaggaaca
     3241 gtttcgacac aaggacaaaa gttcattctg ggagcagatg aaggcggacg tgatgtatta
     3301 ggacaattga tcataggtgc tagaaactct gttacgatcg gatttgcgat cacgatcctt
     3361 acttcgatca tcggggttgg attagggatc atctctggct actacggagg gatcattgac
     3421 aacatcttga tgcggatcgt tgattttatc atgattctac cgatcatgct gatcattatc
     3481 gtcttcgtat cgattatttc cagatacaat gtctggtcct ttatattcat catgagtgca
     3541 ttttattggg tagctaaagc ccgattattc cgaagcaaga cattatctga agcaagaaga
     3601 gattatgtca gtgcctcaaa aacaatggga accaatgatt ttaaaatcat gttccgcgaa
     3661 atcatgccga atctaagttc attaatcatt actaatttga cgatgaactt tgcggcaaac
     3721 atcggtatcg agacaacttt gactttctta ggtttcggct tgccggcaaa tgtacctagt
     3781 ttaggtacat tgatcggtta tgcaagtaac ggagaagtct tagtgaatcg gcaatggatc
     3841 tggttacccg catcaatcct tattttagtt ttgatgttga gtatcaatta tgttggacaa
     3901 gcattcaagc gttctgcaga tgcgcgccaa cggttaggct agtatagaag cgaaggggga
     3961 aataggaatg aagaaaacga tttttggatt tgcagcgctg atgtctgctg tcgttcttgc
     4021 agcatgcggg ggcggaggta cagatacctc tggaaacaac tctggaggca acggcaataa
     4081 caaagaggaa ttaacttttc caattgcaac aaaaaataat gatgatgcaa tcgaagacgg
     4141 cgaattagaa gctgcggtcg caacggacag tcagtttaaa ggattgttcc agtgggagtt
     4201 ttaccaagat tcatatgatg cagcttttat ggctccttct catgaaccgc tatttgcaaa
     4261 cgatgacgat ttcagaatca cagacgacgg agctgcaacg cttgatctag atgaagatac
     4321 gaaaaaagcg acaatcaaag taaaagacaa cgtcaaatgg tcagatggtg aagatgtaac
     4381 ggcggacgat gtgattttcc cttacgaaat cattgggaat aaagattaca ctggtatccg
     4441 ttatgacgac actttccgta acatcgtagg tatggaagaa tatcatgatg gaagtgccga
     4501 cacaatctcc ggaatcaaaa aaatagacga caaaacagtt gaaatcgaat ataaagaagt
     4561 aaacccaagc atgcttcaat cgggtggtgg tatctggtca tatgcagcac caaaacatac
     4621 gttagaaggt gtagcaatca aagatatgga atcaagcgat caagtacgta aaaatccagt
     4681 gacttttggc ccttattaca tgagtaatat cgttgcagga gaatcagtag aatacttgcc
     4741 aaacgagcac tactggaatg gtacgcctaa attgaaaaag atcacgatga aatctgtacc
     4801 gatggcaagt gcaactgaag cattgaactc taaattatat gatatgattt tccaaatgcc
     4861 aacagatact tacgatacct ataaagaaat tgctggctat acaaacttag gccgtgaaca
     4921 aacttcttat acttatctag gattcaagtt aggtaaatgg aatgcagata caaagagtgt
     4981 ggaatacaat ccagattcaa aaatggctga taaatcatta cgtcaagcaa tgggctatgc
     5041 attagataat aatgcagtag gtgaacgttt ctatgcaggt ttgagaagca atgcgacttc
     5101 attgattcct ccagtattcc agtcatacca tgatgatgag atgaaaggat tcacacaaga
     5161 tctagacaaa gcgaaaaaac tattagatga tgctggctac aaagatgtgg atgacgatgg
     5221 tttaagagaa gacaaagatg gaaataaact aacaatcaac tttgcttcga tgtccggtgg
     5281 ggaaacagcg caaccattgg cagattacta tatccaacaa tggaaagaaa ttggtttgga
     5341 tgtccaatac acaacaggtc gtctgatcga attcaattct ttctatgata aattagaaaa
     5401 cgatgatcct gaaattgatg tttaccaagc tgcttggaat acaggaactg atccaaatcc
     5461 aagtggacta tggggaccaa atgctgcgtt caactacact cgttatgaat cagaagaaaa
     5521 tacaaaattg atcaacgata tcagttcaag caaagcattt gatgaagagt accaaaaaga
     5581 agcatatcaa aaatggcaac aatatgcatt tgaagaagca tttgcgattc caacgttgtt
     5641 ccgtaatgaa gtattgccag tcaacgaccg tgtcaaagac tgggattggt cttatgatgt
     5701 accaaaccca tgggcaaatg tttctgttac agcagattca cgttcataat cactaaaaat
     5761 gaccgaaaaa gggcgatgtc ctttttcggt tttttgatat ttttctgttt atttctctaa
     5821 tattcaggtc aaggcgctga tttcatgact tctccaataa acggtgttca aagtccagtt
     5881 tctcggtatt aagtcaaatt tatgcaaaaa tatggaaagc cattttcaca aaattttttc
     5941 ttcatactcg aaactaaacg aatttttcac aacttctcgt caatcactag gattattaaa
     6001 gctttactta ccttttgacc gaaccatttc aaatttgaca aaaaaccagt ataataagga
     6061 agtatcgaaa aaatatccat aaaaggaatg tggaaaatgg acaaccagct gacaaaagaa
     6121 ctaaaagaaa actataacat cgtttttcac gatctaaatt tgcttgaaca agcttttacc
     6181 cattcatcct atgtgaatga gcatcgcaat ctgcaattat cggataatga gcgacttgaa
     6241 tttttaggag atgccgtttt agaactgatg gtttctcagt atttattccg tttatatcct
     6301 catttgccag aaggaaaatt gacaaaaaca cgagctgcga tcgttcgcga agacagcttg
     6361 tcgaaatttg ccaaagaatg tcactttgat caatatgttt tgttgggcaa gggagaagag
     6421 aattcaggag gacgtacgcg accagcattg ctttgcgacc tattcgaagc ttttttaggc
     6481 gcgttatatc ttgaccaggg attcgacacg acccatgcat tcatcgaaaa agtgattttt
     6541 cctaaagtca aagcaggtgc tttttcacat gagatggatc ataaaacaaa actacaagaa
     6601 gtcttgcaaa aatcaggtga tgtatcaatc gaataccgat tgatcaatga agaaggtcca
     6661 gctcatgaac gagtattttg gatcgaagtt tatgtagatg atcaattgat tggcactgga
     6721 caagggaaat cgaaaaaatt agcagaacaa gctgcggcgg aaaatgcatt ggcagcactg
     6781 taaaagtaag tgagggaaag ctttgtattt gaaacgaatt gaaatagccg gttttaaatc
     6841 atttgcagac aagacagtca ttgattttga aaatagcgtc acagcagtgg ttggaccaaa
     6901 tggcagcgga aaaagcaata ttacagaggc tatccgctgg gtattaggag aacaatctgc
     6961 taaaagtctg cgaggcggaa aaatgccgga tatcatcttt gccggttccg atacccgtaa
     7021 gcagctgaat attgcagaag tcacagtgat tttagacaat agtgatcatt atctgccatt
     7081 ggagtacaat gagatcagtg taacccgccg ttatcgacgg actggggaaa gcgagttttt
     7141 catcaataaa caaagctgca gattaaaaga tatccaagaa ttgttcttag attctggatt
     7201 aggaaaagaa tcgttctcaa tcatttctca aggaaaagta gaagcgattt tcagtagtaa
     7261 accggaagac cgtcgtggga tctttgaaga agcagctggt gtcttgaaat acaagcagcg
     7321 gaaaaagaaa gcagaacaga agctttttga aacagaagac aacctcagcc gcgtccaaga
     7381 catcatccac gaattggaag aacagttaac tcctttggcc gcccagagtg aggcagcaaa
     7441 ggagttttta cggctaaaag aaacactgac tcaaaccgat gtctctttga tggttgctga
     7501 aatcaagaca gcaaaaaaag attgggacaa caaacaagca cagcttgcga aattcaatct
     7561 agaattaggc aaactatctg agtcgatcca agaacaagaa tccattttgg cgaagcagcg
     7621 taaagaaaat gcgcaagccg atcgtctgat cgaaaaaaat cagcaagtat tattagatct
     7681 ttcggaaaaa ctgaagcaga cagaaggaca aaaagatgtt ttgcaggagc ggacaaagca
     7741 tacacaaaaa agctcgcaag aatatcaaac ttcattagca gaagcacaaa aaaaggttaa
     7801 acattttgaa aagcttcaag aaagcttgat gaaagcagca gcagaaaaag aaacagagat
     7861 ccaaaaagct gaagcaaatc tcataaaaac acagcaagaa ttagaaaaat accaaaaatc
     7921 aacgaaagaa ttattggcag aattaagaga tcaatatgtt gatttgatgc aagaacaagc
     7981 agctgtagga aacgaactga agtatctaga acgtcaatat atacaagaaa cagcaaaaag
     8041 caagcagacg ttagccaagc aatcggaagt agaagcatca gtagaccgat tgatcttaca
     8101 aaaagaagaa ttgacacaaa agcaggcgca gctaaaatca tctttaactg aaacaaaaga
     8161 gaagttagaa atgatccaac aaaacgggaa aaagttccaa gaagcattag caaaagaaca
     8221 gcctaaaatg tatcaattaa tgaatcaagt ccagcagcta cgtgctcgta agaaaagcct
     8281 tcaagaaatc caagaaaact attttggttt ttaccaaggg gttcgattgg tcttgcagca
     8341 taaacagcag ctttcgggta ttgtcggagc agtggctgaa ctgatcgatg ttcctgcaga
     8401 tttcacgtta gctatcgaag cagctttagg tggggcagcc cagcatgtca tcgttgaaaa
     8461 tgaaaaagat gctcggcaag ccatcacgta tttgaaacag caaagaggag gaagagcaac
     8521 attccttcca ctgacgacaa tcaaaccaag acaattaccg gctcatatat tgacacaagc
     8581 agcagctgta gaagggttta tagggattgc cagtgaacaa gtcagttatc ccgatcaaat
     8641 ccaaactgtg gttcaaaacc tgctaggaac gatcctttta gccaaagact taactagtgc
     8701 caatgcaatc gcgcaaacga tccgctatca atatcgagtg gtttccttag aaggtgatgt
     8761 gatgaatgcc ggaggatcca tgacaggtgg agcgaataaa cgaggaaacc aaggcagttt
     8821 gtttgtacaa aatcaagaat tgaaacagtt gactagtgaa tttgaagaag cagataaaca
     8881 actccaagca caagaaaaaa aagttcagga gctgcaacaa gaaacagctc gtttagccga
     8941 agaacaagaa gttttgcgta cacgaggcga acaattacgt tttgaagaac aagaagcgac
     9001 aaatcagcta caaaacatca taaatgaact tgaacgtttt gaaaaagaaa agcaaatctc
     9061 aacttttgag acacgtgaac tgcagcagtt tatcgaagat tacgaaaagc aaacgaacga
     9121 attgaaagac aaacaaacag atcttgaatc gcaacgacaa aaaatcgacg aagaaatcaa
     9181 atcgttaagt caagaaagcg accagatgga agccagaaga gcacaagtac aaagtcaaaa
     9241 agcacaagaa caagcagatc ttgctgtctt gaaagagcag tttaatcatc tgcagatcca
     9301 gctccgcggt gcccgagtcc aaaaagcaga ggcaaccgaa cgtcaagaag cgatagaaaa
     9361 gcagttggcg acattgacag cagacttttc tgatcatgaa gtcacagaag aaagtttgga
     9421 aaaacaaatt aacgaattat ctgctcagcg agaaacatta aaagccgagt tggcaaaagc
     9481 caaagagcag cgagatagaa ggcaaaagga gatcgataag cttgaagcag tcttggcaga
     9541 gcgtaatcgt gagcaaaaag cacgactgtc ggaacaatcc aaattagaag tccaaaaaga
     9601 ccgagctgaa atgattttag ataatcattt gacctatctg cagtcagaat atcagctgac
     9661 atttgagaaa gcttcccaag actatcaaga aacaaccgat atcgaagaca gtagaacaaa
     9721 ggtttcatcc ttaaaagaac agattgaaaa actgggacca gtaaacttaa attcaatcga
     9781 acaatacgaa caagtcagtg aaagacatac attcttagca acacagcgag atgatctgtt
     9841 agcagcgaaa aaccaattgt ttgaaacgat ggatgagatg gatgatgaag tgcggacaag
     9901 attcaaagaa gtatttgaag ctatccgtca agaattcaaa attgtctttc caaatatgtt
     9961 tggcggcggt aaagctgaat tagtactgac ggaaccaact gatttgctga aaacaggtat
    10021 tgagattgaa gtacaacctc ctggcaaaaa actgcaaagt ctaagtttgc tttctggagg
    10081 agaacgagca cttacagcca tcgcgttgct cttttccatt atccgtgtct gtcctgttcc
    10141 gttttgtatc ttagatgagg tagaagcagc gttagatgaa gccaacgtga aacgttttgg
    10201 tcgttacttg agcgatttcc aagatgacac acaatttatc gtagtcacac accgcaaagg
    10261 aacgatggtc gcagcagatg tactgtacgg agtgacgatg caggaatccg gtgtatcaaa
    10321 aatcgtttct gtacggatgg aagatatcaa tgaagaggcg aaaatcaacg aaacgaaagt
    10381 gtaggaacga aaatgatcaa attagtagca atcgatttgg acggtacatt attaaatgaa
    10441 caaaagctga tttcagatga gaataaacaa gcattagcac aagcaaaaca gcaaggggtc
    10501 aagatcgttc tatgtacagg acgcccctta gctgcgatgg cgcattatct gcaagaatta
    10561 ggactggtag atgaaggtga ctacagcatc actttcaacg gcggtttggt acaaaaaaat
    10621 gatactggtg aaatcatgga aaagaaagtg atggaagtat cagatatcca acgcttatac
    10681 aaactggctc agcaattgga tctgccacta gatgttttgt ctgacaatgt ggttttacag
    10741 ctgcctagtg ccccaaggaa gcaatccttg tacaatgtgt tgaataagct gctgcaattc
    10801 caaccagcgg atataagcga tattacagat gaatttgtgc ttaataaggc agtaatcgct
    10861 tatgaccaag aagaattaga tcctaaaatc aaagaaatac cagcagaatt ccatgaaatg
    10921 tatgagatca tcaaaacaag aagtatgctt ttggaattta tgccaaaagg tgttacaaaa
    10981 gcttatggta tttctttatt ggcaaaagat cttggtttga aacaagaaga aatcatggcg
    11041 atcggtgatg aggaaaatga tttaccgatg atccaatatg ctggactagg cgtagcgatg
    11101 gagaatgctg tgcctttcgt caaagaagca gcagatttcg tgacagccag caatgaagaa
    11161 aatggcgtag ccaaagtgat ccaaaaattt gtattagagt agggggagtc aaatgggatt
    11221 ttttgataaa atcaagcgtg cttttactgg cgaagagaaa acagaagaag ttgaagtaca
    11281 ggagaagtac gacaagggtt tagaaaagac gcgtaaaaca tttggtgaac ggatgaatga
    11341 gttgtttgcc aattttcgaa cagtcgatga agactttttt gaagaattag aagaaacact
    11401 tatcggtgca gatgtaggtt ttgaaacagc gatacgaatc accgaagcat tacgtcaaga
    11461 agtgaaactc agaaatgcga aaaaatcggc agaagtccaa aatgcgatca tcgagaaact
    11521 tgtggatctc tatgaagcgg aaggaatcaa tgaagtaaat gaactaaata tccaagaaga
    11581 cggactaaca gttattttgt ttgtcggtgt taatggtgta gggaaaacaa caagtattgg
    11641 taaacttgcc catcagttca aaaacgaagg taaaaaagta ttgatggcag cagcagatac
    11701 cttccgcgct ggagcaatcg accagttagt tgtctggggc gaacgcgctg gtgttgaagt
    11761 tgttcgtggc aatgcaggag gcgatccagc agctgtcgta ttcgatgctt tggaaagagc
    11821 aaagacagag caggcagatg ttttactagt tgatactgca ggccgactgc aaaataaagt
    11881 gaacttgatg aacgaattag aaaaaataaa acgagtaatc cagcgggaat taccagaagc
    11941 accccaagaa acacttcttg tattagatgc aaccactggt caaaacgcga tggttcaagc
    12001 aaaacaattc aaagaaacaa cagatgtgac cggtcttgtt ttgacaaaat tagatggtac
    12061 agcaaagggc ggtatcgtac ttgctatccg taatgaattg catttaccag tgaaattggt
    12121 cggtcttggt gaaggaatcg atgacttgga gccgtttgat ccaaatgatt ttgtcgttgg
    12181 attgttcaaa ggattactaa aagaagaata acccatgtct aaaaagcagg agctgtgaca
    12241 aaagttgcag ctccttgttt gaatgattgt ttttaacata gctgtgtttt tgatgccttc
    12301 atttttatac ttatttgtaa gcggttctca aacaatttcg ttctttttct tagacttttt
    12361 gattataatt agattcatat gaattggagg tcacttaatg atgaatttta agcaattatt
    12421 attgcatgtc aatgcgcgcc cttttatcga ccaagctcga tttggtatag aacgggaagg
    12481 acagcgggtt gatcttgcag gaaatctagc aaaaaccgat catccagcaa tctttggcga
    12541 tcgatcctat cacccctata tccaaacaga ttttagcgaa acacaaacag agatgatcac
    12601 ccctgttacc gattctattc ccgaattatt tcagtatctc gctgctgttt atgatgtgac
    12661 tgctcgttct ataccgaaag aagagatgat ctggccatta agtatgccac ctgccttacc
    12721 ggaaaaagac gaagagatca ttattgcaaa attaaaaaat ttcgaagatg tcttgtatcg
    12781 acgttattta gcaaaaaaat acgggaagcg aaaacagatg gtaagcggta ttcatttcaa
    12841 ttttgaattt ggcgatgagt tgctaagaac attgttcagc catcaggaag aatttcaaga
    12901 tttttccgaa ttcaaaacag aactttattt gaaaacagcg agaaacttta tgcgctatcg
    12961 ctggatgatc acttatttat tcggtgcttc accaatgagt gagaagaact actttttaga
    13021 tgaatcacat ccgcaagaac ctgttcgcag tattcgcaac agtgcgctgg gctatacgaa
    13081 tcatccaaat gtgaaagttt cctatgcttc tatgaaacag tatttagcag atatcgagcg
    13141 aatgatcgaa gaagggaaac tttcggagga gaaagaattt tatacaccgc ttcgtttccg
    13201 aggcggaaag aaagtcgcag atttagcaac aacaggtgtt cgttatattg aattgcggaa
    13261 tatcgatttg aatccttatg caagattggg tatcaatcca gagcaagttc ggttcttaca
    13321 attattcctg atgtacatgt tatggacaga agaaaaagaa gactgtgacc aatgggtagc
    13381 agaaggtacg actcgaaata acaaagtagc actagaacaa ccatcagatc aaacagaatt
    13441 tcatcaagaa ggtagagaaa ttcttgaagg aatgaagcaa atgctggttg aattggattg
    13501 gttggattct ctttatttag tagaggaagc attgactcaa atggatcatc ctgaacaaac
    13561 gttagcagcg aaactctatc aagaagcaca gctatcgagt cagcaagaag ttgctgtggc
    13621 attgggacat caatattata aagaaagcca tgaacgtccc tatcagttgg ccggttttcg
    13681 cgaaatggaa ctttctactc agatatttat gttcgatgcc atccaaaaag gtgtccaagt
    13741 caaagtatta gatgagtcgg atcaattttt gcgtctgcaa ttccaagacc atgtagaata
    13801 tgtgaaaaat gcaaacatga caagtaagga cagctatatc gtcccactta tcatggaaaa
    13861 caaaacagta acgaaaaaag tcttaaaaga agctgggttc cgggtaccag gaggcgcaga
    13921 attttcatcc atggaagaag cggtaaaagc ttatccgaga tttgccgagc aggcatttgt
    13981 gatcaaacct aaatcaacca attacgggtt aggtatcacg attttcaagg aaggagctag
    14041 tctggaagat tatcaagcag gtttagccat tgcttttcgt gaagacagtt cggttttagt
    14101 agaagaattt atgccgggaa cggaatatcg tttctttgtg atcgacggag aagtccaagc
    14161 catcatgttg cgagtccctg ctaatgttat tggtgacagt atccgaacag tgaaagaact
    14221 tgttgaagaa aaaaatagtg accctttgcg tggaacgaat catcgtgcac ctttagaatt
    14281 gatccaatta ggtgaattag aacaattgat gttgaaagaa caagggttga cgatcgaatc
    14341 agttccccaa gccaatcaaa ttgtttatct aagggagaac tcaaatatca gtacaggtgg
    14401 tgattcgatc gatatgactg atgaattttc tgaagcttac aaaaaaatcg ccgtttctgc
    14461 ggtagaagca ctaggagcta agattagtgg tatcgatttg attataccag ataaagaaat
    14521 cgatcctaca actgataaaa aagcatatgg gatcatcgaa gcaaatttca atccagccat
    14581 gcatatgcat gtttatcctt ttgcaggaaa aggcagaaga ttgacgatga atgtattgaa
    14641 attgttatat cctgaagtat tttaaaagaa aaagcttttg acagattgtc ctatttgatg
    14701 tcaatctgat caaaagcttt ttttgcatgt aaaaatttat gtgttttttc gttttaaaat
    14761 aaacgatccc agaagctttt tttctttttt ggtttttctt tttcagctgg cgaagagttt
    14821 tcttctgggt attttttttc gatatcaatc acaggttcat ttactgcttc ttttgctgtt
    14881 aagagcaatc cgaattcctc aggatttgca gtgacgtaat cgttgactac agtgaaacgg
    14941 ctattgtttt tcgttgctag ctggatatat tggttttgga cagaaagggg tagagcccca
    15001 ttcaagagta tggaagcatc tggatgtttt ttgagttctt ttaaaaagtt ttctttgttt
    15061 tttggttgga tcatttcagc aacagtcata ctaagatagc agcgttccct gaatgtccct
    15121 aaatatttat gctgttcttc agggttcact aaaggtgtac cataaagtcc cttatccaaa
    15181 tggcttttta catcatcttg tgccataaaa aataccctcc ttcttctctt tgtctttctt
    15241 acgattatag catattccca ataataccta aaacatatga aaaagcggtt gcttttgttc
    15301 aacgattcac ttaaaataag aatgaaaatc attctcaatt agaaaggtgg attggatgcg
    15361 ctacggacga ctagtagcat tattagttat tttaagtagt atttcattgt tcatcggagt
    15421 atcgaatgta caattgtcag aactgctcca gttagatggg caaagcttac aaatcttgct
    15481 agtgactcgt ttgccacgaa cgatttgttt gatccttgtg ggagcgacca gtagtatttg
    15541 tgggttgatc atgcaacatt tgacccaaaa taaatttgtt tcaccaacga ctgcgggcac
    15601 gatggatagt gcgcgtttag gtattttagt ggctatgatt ttcctacctg gtgcatcctt
    15661 gattgtccga tccttgacag cattttgctt tgcttttgca ggaacattgc tttttttatc
    15721 ccttacgcga ttgttcctcc agaaaaatca attgatgctt ccgttgattg gagtcatgtt
    15781 agggaatatc attggttcag ttgctacttt ttttgcttat caattccagt tggtacaaaa
    15841 tatgacttca tggcttcaag ggaattttgc gactgtgata cgaggaaatt atgagttatt
    15901 gtatgctgtt gttccattgt tgctcttggt ttggctattt gcttaccgat ttacgatcat
    15961 tggtctgggc gaatcgtttg cagtaaatct gggaatagat taccagaaga tgcagttttt
    16021 aggcattggc ttagtatcat tggctagtgc agtcatgttg atcatggtag gaagcattcc
    16081 ttttatcgga gtgatcattc cgaatcttgt ctctagaata tatggagatc aagtgcacaa
    16141 aacaattggc gtaactgctt tagcaggtag tttgttctta gtattttgcg atcttgtcgc
    16201 tcgcgtgttg atttttcctt atgaaatacc agtcagtgtg atcgttggaa tcatcggtgg
    16261 ggctatcttc ctttatcttc tgataagggg acgacgatat gcttaaaaaa aagccatcta
    16321 tttttattgg tatgcttgtt cttgttttat ttgttacagg aatgtacttg acgatccata
    16381 catatggaaa ctggcatttt gcatgtgcat tgaggagtaa gaaaattttt gcatttatcc
    16441 ttgtctcgct agctgtcagc agtgccacaa tcagctttca gacaattacg cataaccagt
    16501 ttttgactcc ttcagtattg ggacttgagt cgttatatgt ccttgtccaa acggtccttt
    16561 tcttctttat cggaggagtg acgatgcttc agcagacagg tacagttttt ttcttattga
    16621 acgttgggct gatggtcggg ctgagtctat tgttattttt ctttcttttg aaaaaaatga
    16681 gagcgaatct ttttttatta ctgatgtcag ggatgatttt aggtacgctt tttggtagtg
    16741 tcagtacctt tttacaagtc atcatggatc cgaatgaata tgatttgctt caaggcagat
    16801 tatttgcaag ctttggcaat atccagagtc agtatctttt gcctgcttct ttgatcatta
    16861 tcggctgctg gtacctgtta tttcgatatc gacatgtgct agacgttttc catctcggaa
    16921 atgaacaagc agtaaacctc gggatagaca cagatcgttt tgaatttttg attttatgtt
    16981 gtgtcagtct tttgacaggg acatcaacgg ctcttgtggg tccagttact tttttagggt
    17041 ttattgtcgc aaatatcagt tatcgtgtct tttctactta tcgtcatggt cagctattta
    17101 tcggaagctt tctggtagct atgctgtttc tgacaggagg acagctcgca gtagagcaga
    17161 ttttcggctg gaatacaaca gtgagtgtcg tcgttgaatt tgtcggagga atttattttg
    17221 tcacaaaatt gattcgcgaa aggaagagta tcctatgatt gatttacaaa aggtcaataa
    17281 actgtaccat ggaaaatcag tggttaaaga tgtacagtta acgattcctg aaaagaagtt
    17341 gactgctttt atcggtccaa atggtgctgg aaagtcaacg gtgttgtcga tgatcagtcg
    17401 tttgatacca aaagatacag gtgaaatcta tttagaccat aacgaagtaa aggcttggaa
    17461 atccaatgaa ttagccaaaa aattatcgat cctacgacaa tcgaatgaaa taaatgtcca
    17521 aatcaccgtg aaagaattag tagaatttgg acgatttcct tatacgaaag gaaaattgac
    17581 gacaaaagac gaggaaatcg ttcaggaatc actggaacac cttgggttag aagaaatagc
    17641 ggatcaatcg attgacactc tttcaggcgg tcaactccaa cgtgcgtata ttgcaatggt
    17701 gctagcgcaa gacacggatt atattttact ggatgaacct ttgaacaatc tagatatgaa
    17761 ttttgcggtt caaatcatgc agattttaaa aaatttagta caagaaatcg ggaaaacaat
    17821 tgttattgtt cttcatgata tcaactttgc tgcaagttat gcagacgaaa ttgttgcaat
    17881 gaaagatgga cagttattta cgcaaggaac gacccaagaa atgataaaaa aagatgtatt
    17941 ggatgaatta tacgaaatgg acatacggat ttgtgagatt gaaggccgca gattctgtct
    18001 ttatttctaa aactaacatt aggagagaag agatgaaaaa attagtagta ggtatcttga
    18061 ttctagcatc atttggttta gcagcatgtg gaaatagcaa caacaccagt caggcagaca
    18121 caaaaaaaag cagcacacaa actgaaacta cattgacgat aacagatagt aatggagacc
    18181 aaatcgaggt gccaaataat ccagaaaaag tcgtcgtatt cgacaatggt tctttagata
    18241 cgatggatgc tcttggtgtt ggagataagg tagttggagc agctacaagc agccttcctg
    18301 agtatctttc atcctataaa aaagtagaat cagcaggtgg aataaaagaa ccagacttag
    18361 aaaaaattaa tcagcttcag ccagatctaa tcatcatttc tggccgacaa agagattttc
    18421 aaaaagatct aagtcaaatt gctccgacca ttttcctttc tttagatgcg aaaaatccat
    18481 gggaatcttt ccagcaaaat gtcactgctt taggggagat ctttggtaag caagaagaag
    18541 ctaaaactca attagaggaa ttatctagtg caatcgatca gacgaaaaag aaagcagaag
    18601 ctactgacaa aaaagcacta gtcacacttg tgaatgaagg gcaattatca gcctacggat
    18661 ctggttctag attcggtttc atccatgatt tatttggctt tgaacaagca gatgatcaaa
    18721 tcgaagcatc cactcacggt caaagtgttt cttatgaata tgtgctggaa aaaaatccgg
    18781 atatcttatt tgtagtagac agaacaaaag ctattggtgg agacgacagc aaagatgata
    18841 tttctgccaa tgaactggta gcacaaacga atgctggtaa aaatcagcag atcatctccc
    18901 ttgaaccaga tgtctggtat ttaagcgggg gcggtttaga atcaatgaaa ctgatgatcg
    18961 aagatgtgaa tcaagcattt aaatagcata aataacagag gattaaactc tcatactatg
    19021 taacaataat gtttcttaca ttcttaaata gcaagagcct atcctcaaag aagttatcaa
    19081 aaaccatccg taaaatcgta ttgatgattt tacggatggt ttttatttat ttgagaaatc
    19141 ttttttcaac gtgaattcat tatctacatc atacgggttg attctagaat ggggagtggc
    19201 aaaaaatagt gtgatccatt ttttcagcat caagatcgcc tcctttgatt tgaatcaaag
    19261 gtacaaagcg aaacttagat aaaacttaaa tttagatatt tttattttgt accgatctct
    19321 tctttaaacg atgttccatc tgctgcttct cggcaataag tccactagac tagaaaatat
    19381 gagaagctat tttctgtcca gcgttcttat tactcaaaac aaatcgcagt tgtcacaatc
    19441 tcttctaaac gatgttccat ctgctgcttc tcggcaataa gcccactaga ctagaaaata
    19501 tgagaagcta ttttctgtcc agcgttctta ttactcaaag caaatcgcag ttgtcacaat
    19561 ctcttctaaa caatgttcaa tcagctgacc ttcggtatta gctcaaagca ctgatttcac
    19621 aacctctatt taggcatgtg tctcagcaag cggttgatag ttgatgatct gcatttttcg
    19681 ataaaaatcg gcgggaagct tactccaccg ttttagtaat tgatagaaat aactctcttc
    19741 tgctttgatt ttatactcgc ttaatatcaa tccaaaacta ttcgtttttt ttgaagctaa
    19801 ttttttataa atatccaatg ctttggtttt atcttcattg acaaagagtg tttttacttc
    19861 aatataagaa ccctcgatat tttgtgtttc ttcgataaaa tagatgtttt ttatatattc
    19921 cttcatagtg attcctccaa agaagtcttt tatttgataa gtttactcta ccgaagagtt
    19981 gttaataatc cgtgaacaaa aagtaattct ttttccaata gttgttaaag gttttttatt
    20041 ttttatagta ttttttgtta tttaagaaag gaataaggtt aaacaaataa caggttgctt
    20101 tttgggtctt tttccgttca agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg
    20161 ataagagaaa tcataataaa cgaatgactt actttccaat aaattgagaa cagtcagaac
    20221 cagacaaatt agttgcatct gatgaataaa gtgattattc tatgacatga ggtgatacag
    20281 atgaaattag aagtgacaaa agaagcacaa gaagtattga agaataaatt agaagcagac
    20341 gatcaacttt tattagatat cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt
    20401 tgccaattgg atacggcatt tcgattgatc attgtcaaaa aagatacaca aacagatcta
    20461 agtctttatt ctgtagtggc tgatacacca gttggaccaa tcaaaatcaa agacagtgca
    20521 atactttaca tggatgatcc atccacactt gctttagaac caacttataa tagtttacaa
    20581 ttaaaaggac caagcggctt gagaaaaggc aacttgcaag ttgttcgaaa agactagagg
    20641 gataaaatgg gaaaattggc gatcatcagc gacctacatg tggacatcaa taaattcggc
    20701 gaatcagagc tgatgcagct ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat
    20761 ctagccggag atacggctaa taaagtagac cgttgcctag ctgttgtcga atttttcgtc
    20821 aataaaggat tatctacgac atttcatttc ggcaatcacg agttggcaga tgtgcccaat
    20881 gaacagatga tggaccattt tccagaccca cattttttaa atgaacggta tattgcttta
    20941 aatgagcaga ccgttctttt agggatgaat ggctggtacg actatcagtt ttccgaattg
    21001 aaagattacg aggcaatcgt acgaatgaag cgtttgtatt ggtatgatcg gttcatccag
    21061 cgtgggaaaa cagatctcga ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta
    21121 gaccagctgg aagcaaaaaa acttgaggtg attttatcca ctcatttcgt tcctaaacgg
    21181 gcatttatca tgtatcaatc tgctccttat gagcgttgga acaaattaaa tgcattttta
    21241 ggatcagaat cattcggaga attacttgac cgttattcaa atatcaaaca agtagttttt
    21301 ggccatacgc atcgtcgctt tgaagataaa tccatcggtc acacgattta cagctgcaga
    21361 ccttttggtt attattatga atggcagctg acccgagatt ttgtcttgaa agaacagttg
    21421 ctagacagct atgatcctat gaaattacgt gtcctcttgc gtgcccatca cccagctttt
    21481 caggcctatc aaacacaaca tttgcaagag gaatttgaac aagcgatgac aatcgttccg
    21541 tattgagaca gcattagtaa tgtgtaaaag aaaatacctt gtaaaacata aggataagta
    21601 agagcagtag atccatttat cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa
    21661 tgtttatttg atcggatgat ttgtaactcg cagagtacta gatgcaagcg tgtattcttt
    21721 ttttgaacga taatcacaaa aaaaaatata atatacattt tttatttaat tataattaat
    21781 attgattgaa gtctaggcag tcaagtcaca ttttgataga taggaggggg acttacgaat
    21841 tattttaaag gctatgacag ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa
    21901 actagaaatc aaatcgggct gataatcatt tataactaat cgatacatag gaccaaatat
    21961 aggtctatgt tcttttattt cataagtatc gtaaaacaga attttgctat ggattaatcc
    22021 aatcgactga tatgtttagt aaataaagaa agaggaatgt caatgatgaa aaaagaaatc
    22081 gtattaacag cagcaatgat gttattcagc atggtagcaa gtacgacttt tgtttctgca
    22141 actgaagtat acccaaagga atataacaca gaaggaacga ttacatttga agcaggagat
    22201 gagggtgtaa ctcctcctgt tgatccagag aatcctgatc ccaataaacc tgtcgatcca
    22261 tcagatcctc caagccctgg aacaggaggg gcattatcga ttgattatgg atcaaaattc
    22321 aaatttggga ctcaaaaaat ttctactgcg gataaaacgt attatgcagc tgctgatgtg
    22381 atgaacgatg ggtctagaaa accgacatat gtccaagtga cagataggag aagtacctta
    22441 tctaggtgga aattaagtgt ctctcaacca gaacagttta aaactgccag tggggatgaa
    22501 ctagttggag cacagctgaa atttacgaaa gggcaggctg tttctttggt tgatccaaca
    22561 tatacgccgc aaacggtgaa ttctgaatta acgttgacac cgggaggaaa caatacgtta
    22621 gcaattaatg ctaaatcagg aacaggtgtg gggacttggg tctatcgatt tggtgcaaat
    22681 gaaaatgaaa atcaagatgc ggtacagctt tcggtacccg gtaaatctgt caagttagcg
    22741 caacaatatt ccactaaatt agtttggaca ttagaagata cacctaacaa ctagtttact
    22801 gaaaagaact gttaaaaata aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa
    22861 attgtcaaag gctaggctaa atttgtttgg atacgaatag ttcatctagg aatttaacga
    22921 tcaggaggaa aagatatatt gaaaaagagg aggatcgtgc tcattttaag cagtctctta
    22981 gtgactaata tctttccgat catagggtac tctttggaat tgaatcagtt ggagcatgga
    23041 aatagaagag acgaagaaag caaactcact tcgacattcg acagtttaat agaatccaac
    23101 cagacacaag aaagcagtga ggatttaagt atttcgagta gtggagagac cactgacaac
    23161 gtagaagctg agggttcgga caaacaagga ctagaagatg caccaatttc tgcggttgag
    23221 ctgcctgacg atcccaatat tatttctatc gataaaattt ttcaaacacc tatcggtact
    23281 agcacgtcca tattggaaaa tgggaagtta ctgcagttga acccagcaga aaggtctcaa
    23341 agaggagcta tttggtcaaa taagaagatt agtttactct cggattttac atttaaatct
    23401 tatttatact taggaaatca acgagataga gcgggagatg gcatgacctt tacattgact
    23461 ggcgatccta gaatggctag taatcccagt caagttattg gatcgccagg tatgggaatt
    23521 ggctcatatt ctactcgttc tggccaacct tacattgcga atgcgctctc gattgagttt
    23581 gatacgtata aaaatagtgg ttcttctgat cgaatggatc gagagataac tgctgataat
    23641 agagggcatg gacatgtcgc ttttgtcacg cctaaaccaa ataataataa ttattctgga
    23701 gagcatactg gcgtgacggt agctcctaca tttctttcta atggcacatg gcgcttgtta
    23761 ataattaaat gggacgcgga gcaaagaagt cttacttatg atttggaagg tgtcggtcgc
    23821 tcttctcgga ttatacctga tctcattagg cagtttggtt ctacagaagt aaattggggg
    23881 tttacttctt caacaggtac tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca
    23941 tcgaatgtca catcaacagc tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct
    24001 gaagctggag gaaacgataa gataactatc agaaatacat tgaaggtcga gggggatttt
    24061 cttgaatata agaaaatgca gactagtatc gacctcccac aagaattaac tattcccgag
    24121 catatcttga tcaatggaaa tcaaattgat gccaaagata tcttgataac cggaaatcga
    24181 gtcacgctaa atctgacaga ttattttatt gctggagata cgttaataat cgaattggag
    24241 acaactttgc aggaagtaat tcctgaaaaa gtactcacta tgcattttga ataccttgaa
    24301 aacgggaatt tgattgagca atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag
    24361 atagatatcc atgtttatta taaagataaa gacagtcaga ttgatattgc tgaaatgaaa
    24421 acaatcactg ggaaaatagg agagaagtat aaagaaactc ctattgatat tgaagggtat
    24481 gtttttgact cggattcagc gaatacagaa ggcatcttct ccgaaaatac agaggatatc
    24541 tatttttatt acagaaaagg caaactttat ctctcaactg ctcctcaata tgtcaatttt
    24601 ggaaaacata aaatatccag tagaccatta agtgaattcg gacagttttc aggtgggttg
    24661 aaaattatag atgaacgggc ttttggaagt tggaaattac aattgaaaca agagaaagtt
    24721 cttacaaatg gagattttga gcttccagaa gctctttcct ttgttacctc tgaaaataca
    24781 acggttatag ggacatccgc tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat
    24841 ttgtcctctc ttttagattc tacagctcat cgtggaatca gaataaatat tcctgtagaa
    24901 aaccaaagaa tcggaacctt tgaggggaaa ctatcttgga tacttgcaga tgtcccagga
    24961 aattaacagg aagagaagaa tataacaaaa ttattgagtt tcaagaaggg cacaggtacc
    25021 ataggctctt gtgtcccaca gagacaagat tttgttatag tcttctctta catatgatta
    25081 aaggagcgat tttcttgaga ccaaaacaac aattatttat aacactccta gttattttag
    25141 gggcactttt taacaatata tcagtgtcag ctagtgcatt taatttttca gtgacgccga
    25201 taacttcaga gaatcaaatc gacaagagaa aaacttactt cgatcttcaa cttgatccgg
    25261 atcaggaagt agaagtaaaa gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg
    25321 atatttctgt gaatagcgca accaccaatt caaatgtgat ggttgaatac ggaaaaaacg
    25381 agattgaaaa agacatgagc ctgatctttg atctggtaga ttatgtgagc tatccacaga
    25441 tagtgacgct aaaacctaaa agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg
    25501 agcgatttga tggtgtattg gctggtggga tcacattcca agaaatccag acggaaaagg
    25561 accaaacaga aactaaggat caaagcttat cgattgagaa tgaatacacc tatattattg
    25621 ctttgttgat gcaacagaca ctgaacgaag ttgctccgaa ccttactctt catgaagtta
    25681 aaccagatca aatcaatgca agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa
    25741 cctatatcaa tcaagtagtt attgaaacaa aaattacaaa aaaaggacat tctgaagttt
    25801 tatatcaaga ggaaaaagaa ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa
    25861 ctgctttaaa tggtcaaccg ctaaccccag gtgaatatca ccttacaatg actgttttag
    25921 gaaatgaaaa tgaaaatgga aaattttcga gaaaaaaagg aaatactacg atcaactata
    25981 ccaatcaatg ggtatttgaa aaagatttta caatagacgg aaaagtagcc aaggaattga
    26041 acacgacgga tgttacgatt aaaaaagaca ataattggat ttatatctta gtagcttcac
    26101 ttttcttatt gtttatttta ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga
    26161 aagaaaagta aggttaagat aacaaatagt aaatttttat tattgtagga tggttgggct
    26221 ctttttataa caatataaaa tataccattt tattaataaa aatgcatact agtaaatgat
    26281 ataattctaa taacaggttt gatcgcgaga aaggccaaat aaaacgctct ccaaaatgtt
    26341 aaaaaaaaga caggacgatg tatattatac aaacaagaat cgctgacaac agtgactcgg
    26401 atccaggaaa tcagttttat ccccagctag ttggtgaatg catggaaagt aatatacgta
    26461 gaaagagctg atgactaatg aattatgagt tttttttaag caaagaagat cacagaagtt
    26521 attcattact taaatattta gaagcaagct caaatttatc agagtcaata tcgaatgttc
    26581 aagaagagtt gtcgctatcg acatttctct tgaaaaaaac gatcgataag ctgaaacatg
    26641 atttacaaaa gttcgatcta gagaaaaatt tgcgagtaac cgtcagtgaa ttggatatat
    26701 gtctggaaat taatggaaaa tgttctagca aagctctgtt atctcgttat ataacggatt
    26761 ctttatctat gaaaatggta ttggctttct ttcaggaaaa atatatctca gtagaaaatt
    26821 ttgctaaaca gcatcacgtg agttattctg tggcttataa agtattacaa agactaaaaa
    26881 gaaatctaaa aaaatatcaa attttttttg aaaagagaaa gtt
//
LOCUS       DAZAID010000030        26807 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000030 DAZAID010000000
VERSION     DAZAID010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.030"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..1451)
                     /locus_tag="ACG9BE_001193"
     CDS             complement(36..1451)
                     /locus_tag="ACG9BE_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3031950.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(1462..2130)
                     /locus_tag="ACG9BE_001194"
     CDS             complement(1462..2130)
                     /locus_tag="ACG9BE_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFN3031951.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2260..2844)
                     /locus_tag="ACG9BE_001195"
     CDS             complement(2260..2844)
                     /locus_tag="ACG9BE_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3031952.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3175..3408)
                     /locus_tag="ACG9BE_001196"
     CDS             complement(3175..3408)
                     /locus_tag="ACG9BE_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFN3031953.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3423..4373)
                     /locus_tag="ACG9BE_001197"
     CDS             complement(3423..4373)
                     /locus_tag="ACG9BE_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFN3031954.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4370..5212)
                     /locus_tag="ACG9BE_001198"
     CDS             complement(4370..5212)
                     /locus_tag="ACG9BE_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFN3031955.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5214..5933)
                     /locus_tag="ACG9BE_001199"
     CDS             complement(5214..5933)
                     /locus_tag="ACG9BE_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN3031956.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            6481..6783
                     /locus_tag="ACG9BE_001200"
     CDS             6481..6783
                     /locus_tag="ACG9BE_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3031957.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            6855..8081
                     /locus_tag="ACG9BE_001201"
     CDS             6855..8081
                     /locus_tag="ACG9BE_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3031958.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(8169..8561)
                     /gene="rpsI"
                     /locus_tag="ACG9BE_001202"
     CDS             complement(8169..8561)
                     /gene="rpsI"
                     /locus_tag="ACG9BE_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFN3031959.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8577..9005)
                     /gene="rplM"
                     /locus_tag="ACG9BE_001203"
     CDS             complement(8577..9005)
                     /gene="rplM"
                     /locus_tag="ACG9BE_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFN3031960.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(9539..10006)
                     /locus_tag="ACG9BE_001204"
     CDS             complement(9539..10006)
                     /locus_tag="ACG9BE_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFN3031961.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(10126..11664)
                     /locus_tag="ACG9BE_001205"
     CDS             complement(10126..11664)
                     /locus_tag="ACG9BE_001205"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFN3031962.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            11744..12430
                     /locus_tag="ACG9BE_001206"
     CDS             11744..12430
                     /locus_tag="ACG9BE_001206"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFN3031963.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(12631..13158)
                     /gene="msrA"
                     /locus_tag="ACG9BE_001207"
     CDS             complement(12631..13158)
                     /gene="msrA"
                     /locus_tag="ACG9BE_001207"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN3031964.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(13242..13919)
                     /gene="pgmB"
                     /locus_tag="ACG9BE_001208"
     CDS             complement(13242..13919)
                     /gene="pgmB"
                     /locus_tag="ACG9BE_001208"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFN3031965.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(13916..15046)
                     /locus_tag="ACG9BE_001209"
     CDS             complement(13916..15046)
                     /locus_tag="ACG9BE_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN3031966.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(15046..17229)
                     /locus_tag="ACG9BE_001210"
     CDS             complement(15046..17229)
                     /locus_tag="ACG9BE_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFN3031967.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17226..18251)
                     /locus_tag="ACG9BE_001211"
     CDS             complement(17226..18251)
                     /locus_tag="ACG9BE_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3031968.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(18296..19123)
                     /locus_tag="ACG9BE_001212"
     CDS             complement(18296..19123)
                     /locus_tag="ACG9BE_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN3031969.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(19120..20187)
                     /locus_tag="ACG9BE_001213"
     CDS             complement(19120..20187)
                     /locus_tag="ACG9BE_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFN3031970.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20213..21049)
                     /locus_tag="ACG9BE_001214"
     CDS             complement(20213..21049)
                     /locus_tag="ACG9BE_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3031971.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(21046..21915)
                     /locus_tag="ACG9BE_001215"
     CDS             complement(21046..21915)
                     /locus_tag="ACG9BE_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3031972.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(21985..23268)
                     /locus_tag="ACG9BE_001216"
     CDS             complement(21985..23268)
                     /locus_tag="ACG9BE_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3031973.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(23282..24967)
                     /locus_tag="ACG9BE_001217"
     CDS             complement(23282..24967)
                     /locus_tag="ACG9BE_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFN3031974.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            25242..26267
                     /locus_tag="ACG9BE_001218"
     CDS             25242..26267
                     /locus_tag="ACG9BE_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3031975.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         8039 a         5404 c         4462 g         8902 t
ORIGIN      
        1 ataatctttt gttttcatat atattctcct caattctatt tcgttatgtc attaaaaatc
       61 tgttctatca aagttgaatc ttctactcta agttttatat ctttttttat tccaccattt
      121 tttaaataaa taattctatt gcatgtttta taaagaaatt ccggatcatg agtagctata
      181 actactgcac ccttttcttt taagtcttta ataaggtcag caatcctttc catattaata
      241 taatccaacc cacttgtagg ttcatcaaat agaaataaat tagtttttga caatgaagct
      301 atagaaacca ataatctttg tttttctcca ccggataaat caaacggtaa ttgatctttc
      361 attttataaa gttccatttt ttttaaaatt ttttcttgct gatctttact aactatttca
      421 ttccccaggg ctaactcgct cctcactgat tctgtgaaaa attggtaatc catttcttgc
      481 attactaaaa acggcgatga caaatttttt ttattttcga ttatctttcc tttggtaggg
      541 cgaataatct tcatgagtat tcttagtaag gttgttttcc ccacaccgtt tttccctacc
      601 aaaccaataa catctccaac tttgacatca aaatttatgt cttttagaat attttcataa
      661 gtaatttctt caacttttaa tacatctgca cttacctttt cgatataggg aacttcaatc
      721 gaactattaa aaatgtcaaa tttccttaat gcgttattgt tttcagtttt taacaactga
      781 atagttgtag tttttacagt tctattttta acaaatagaa cttgatcaat taattctttc
      841 aaatagtaaa aacgatgttc agctataaca atagtaaatc ctttattttt taaatcacaa
      901 agtaagtcag caagtttctt agtcgctttg aagtccaaat ttgaagacgg ttcatccaat
      961 ataattaatt tagagtttag cgataaacta catccaatag aaattttctg ccgttcacca
     1021 cttgatagcg aaaaaatgtt tttatctaat atatttgaaa gatctaataa ttcagctaat
     1081 tcattgattc ttttatccat atgctctttt gtaaatccaa agttttccat cgaaaatact
     1141 aactcactcg ttgtatttgt tgtaaagaat tgtcctctag gatcttgact tacgtagcca
     1201 atattttttg atatactttc ttgtgttaag gattgtagcg agttattttc aaataaaatt
     1261 tccccaacca attttccgtc ataaagtctt ggaataagtc cattcattaa ctttaatagt
     1321 gtggatttcc cactcccaga agaaccagta atcaatgtaa gactgccttt ttttattgaa
     1381 aaattaaggt caacaagcgc ttgttcctta acatccttat atgaaaaaga aacattgtta
     1441 aattcaaaca attctttcac ctcaaatcaa aatacttaag aaaaaaatag taacggaaaa
     1501 caggatgata tagtctaata agctcatgtt tgttttatag taagaacttc ttttcacagg
     1561 agattctatc ccctttgtta ttgcattagc ggtcatttct tcagcaatca tagttgcacg
     1621 tataagaacg ggaactaaca gaaattccaa atattgcaga ggctgaaaaa aagacaagcg
     1681 aatgtttctc atttttaaag attctttaat cattcttatt tcagaacgat aagtcggaaa
     1741 gaatctgatc actactgtaa tacttaacat tatttttttc ggtaaatgta ttttttctaa
     1801 agaggataat aattcattgc tagtatagac actaaccaac aaccatgaga tcattacgac
     1861 cggcaacatt cgaaatacaa tatataagga tgtcgcaaaa aatatagtaa aaaactctat
     1921 cttgatagca tcaataattt tcattactaa aaacagaact gaataaatca agatgaaata
     1981 aatacatgtc tttattttac gaaacatcaa tactaaaact gtagcaataa tgaaacaccc
     2041 cacttgaaat atatttccct ttcctaaaaa aataatgacc ggaaaaaaaa tatttaaagt
     2101 aatcaaggtt aacggattga tttttgacac tctttcacct ccaaaaaata agatcgcact
     2161 gttatcttaa gctgataaaa atgcaccgtt attcagataa cagtgcgatc ttatcaaaaa
     2221 gcataactga gaacaattct caatgcaagc tttttttaat taactcagta attcaatcca
     2281 cccaggaaca gaaaattgat acaatcttct ggttaatagc atagtctttt cttttgaata
     2341 gctatgcttc aatatatcat agaatgtagc gtagaatgag taaatgtaga agtggatttc
     2401 ttcttcatta aagactaaat tttgattgat cattccgata gtctttaact tatctagaca
     2461 acagactgtc tttttcgtca caaatgatgt aattttttca acaatatctt gatatttggt
     2521 tccagctgat tggaataaaa tcaaatcgaa tatacgttta ttctcaaaaa tataattaat
     2581 aaatggatct aattcattga agttctctct ccaacttttc tctatatcta tattttctaa
     2641 gtttaagagg tatttggtaa actcttcatt atacatatta ataagcgcat taatatgcgg
     2701 ctgaataatt tcttcaaata acgcttcctt agaagtaaaa tgtttataaa aagcacccgt
     2761 agttacgcct gcggtcaggc acagttgcct tatcttagtt tgctgaaatc cttctttttc
     2821 aaaatggctt agtccacttt ctattaactt ttggtgagtt ataatataat ctctggtttc
     2881 tatgggtact tcctcctaat aagatgaatt aactttatta tcttacacag gacttgaagt
     2941 ttcaatcgtt aacgtttatt actgaactca ttccttagag ctaaactaat gagaaagagc
     3001 agaataactg atacaaaaag caatcctaca agaattaata gactcatcta aatcattcac
     3061 cttttctaaa ataatccgag aaagatttaa aaaaattata tccagctatt aggataagtc
     3121 ctatcataat cactccaata gccattttct tatcctccct aaaaataaag tcttttactt
     3181 aaaacctttt ttgtaatcgt caataaattt tttcccttta tcagttaatt gaaatcctat
     3241 atctaaatag tcctcatatc taagtttagc cagcatctca cttacagatg ctggactaac
     3301 acttagaact tttgctaaat cattattttt taatttcttt tttctttttg ataattcctg
     3361 aattgctagt aaataaatta gtatagtagt cgttttaatt tgtaccatac ctaccctcca
     3421 aattatttcg aaaggccttc agcaatttta tccaggttcc atttcatcat cgcatagtaa
     3481 ctatcaccat cttgtccttt ttctgcaatt gaatcagtaa aaatcgttga atagatagga
     3541 atattggtat cttttgatac tgttttcatt ggtctatcat ccacactgct ttctacgaat
     3601 aaggagggaa cttttgttgt gcgtaatttt tcaactaagt gttttatttg atctggtgtt
     3661 ccttcttctt cagtgttgat ttcccaaatg taagcagaag gcacattata cgctttcgag
     3721 aaatatttaa agcatccttc acttgtaaca atcatttttt tatcattcgg aattgaagca
     3781 aatttagatt tagcttcttt gtctagagaa tccaattttt caatatactt atctagattt
     3841 tctttataga attttttatt atcaggatct ttttccgcta actgtttttc aatattttta
     3901 gcgtaaataa taccgttttc taaatttaac caagcatgag gatcttcctt ccctttctca
     3961 ctctgaccct ctaagtaaat aacatctatg ccatcacttg ctgcaaaata gtctttgttt
     4021 tcctctttgt tcgcattttt aactaatttt gtaaaccaag catttcctcc agtttccaag
     4081 ttaacaccgt tataaaaaat caaatctgct tttgaagttt tttgaacatc ttcaggcaaa
     4141 ggttcatatt cgtggggatc tttcccaata ggtacgatac tgtgtaaatc aattttatct
     4201 tttgctatat tttcggtaat atctgctaaa atcgaattag taactactac ttttaatttt
     4261 tcgttcgaag ccactttatc tttgtcttta ctatttattg aacatgcccc gaatataaaa
     4321 agagcaatca aaatccccag acttcccagt actgctttcc attttctcat cataacgatc
     4381 tctccttctt tcttaaaaat ttttgttttg gtgagattag aaaactaatt acaaataaga
     4441 atgccgctgt taaaacaata cttgatcctg ctgctatgtt aaaactataa ccaataaaca
     4501 atccaagcaa tgatgaactt gctccgactg ttgcagaaat aaatatcata gttttcaagc
     4561 tatttgcata taaatatgct gtagctgctg gtgtaattaa cattgcaaca attaatatag
     4621 ttcccacact ttgcatagct gttactgcta ctaaagttaa tagaaccatc aatagataat
     4681 gataaaaact gacattcatc cctattgctt ttgccattaa tggatcaaaa gaagtgatta
     4741 ataattcttt aaaaaataga actactataa ccaacactaa aaccgaaacg ccaattgtaa
     4801 tccacttatc aatatcttgt acagctaaaa tatttccaaa taaaatatga aataaatccg
     4861 ttgagctatt tgcaactcct ataaggataa ctcctagtgc taaaaaagaa ctgaaagtaa
     4921 taccaattgc tgtatctcct tttattacac tattgctttt tataaaagta ataatgatag
     4981 aagctaatag accaaataca atcgcaccaa taaaaaaatt aatacctaaa atataagaaa
     5041 ttgctactcc tggcaaaaca gcatgagaaa tagcatcacc cataagagac attcctctta
     5101 aaataataaa acaacctact gttccagaaa caatccctat aactacagat gtaattagag
     5161 cattctgtaa aaagtgaaag tcatacagac cattaataaa attttgaagc atattattcc
     5221 ccctctccaa taaatatagt atctccatag gcttttttta aattttcttc attaaataca
     5281 gattctacac ttccgtgagc aattagattt cgattcaaaa gaataatatt atcaaaatac
     5341 ttctttacct tgcttaaatc atgatgcaca attagcactg tttttccctg atgtttcaat
     5401 ttttttaacg tatccataat tattcgttca ctaactaagt ctatccctac aaatggttca
     5461 tctaaaaata tgaaatccgc attttgagca agacatcttg ctaaaagtac acgttgaaat
     5521 tgtcctccag ataattctcc tatttgatga ttagaatatt ttcccatatc gaccttttcc
     5581 aaagcctttg aaactctttg ccattctgca tttttaattc tttgaaaaac tttcattccg
     5641 gcatatgttc ccatagatac acattctttt acttttattg gaaaagtaaa atcaatatca
     5701 gctttttgct ctacatatgc aatcttcacc agttttttat tgatatcttc attattaatg
     5761 gagacctctc cctgatgagg gataatattt aacattgctt taagcaatgt ggattttcca
     5821 gcaccatttg gaccaataat acctgttaaa gcaccttctt gtatttttaa agaaatctcg
     5881 ttcaatgcta aaaaatcgtt ataacaaaca ttaagctttt tcacttctaa cataactttc
     5941 tccttctaaa tgataatgat tatcacgatc atatggtata caaaaattat attttagtca
     6001 accctaaatt ttatagttta gatttttttc tgttgctgaa gattagaatg acaaaactat
     6061 ttgaaactac gcgtatagta agtagctttc aaaatatata aaaaaggagc gttctcaaat
     6121 gagaacatgc tcctaaaact actttattgg cgtatggaaa aattatactt gaagtggata
     6181 gcgggttgaa taagttgata agcatcaaaa catttataaa aattattcaa gactatgtct
     6241 gttccatata caaatatgaa aatcagtaaa ctccatttaa cttttttcgt agtgtgtctt
     6301 tttatacatg tttatcgtta ttaatagtga ataggaaaaa aactattttt gatcattgac
     6361 aattaaatac aatttttgaa taacgatccc tgctattcga gctagaaact caagtacgcc
     6421 atgacctgat tagcgacaaa tacttagaga tttctttttg ttctgggaaa acaaaatgcc
     6481 atgacaatag cagcaaataa tgatacttcc gtaaggctcg ccgtaaactg gggagaggtg
     6541 aaattcctat gtgcttaccc aaagcaattc aaaaatttca taactactat tttaggagat
     6601 gattttatag aatccagagt ctacctgaat aaaaaacaaa cctgtgcatt tctaggaatc
     6661 gataataata ctctagataa gtggattcgt atatataatt tcccagtaat aagagttggt
     6721 ggtgtagttc gctttgacgt agaagatatt aaagacttta tgaatacatt taaaataact
     6781 tgaaattttc ttctcgccgc atatactaaa attagtgaaa tatactgcct tacttttttt
     6841 aaggagtgag gattttgcct acaaagcttt caaatgggaa atataaaact aatctgagat
     6901 atccaaaaag gtttaaagaa ataacaggaa ttgcatcaga aaagtatcaa aaaacatttc
     6961 caaataggca attggcgatt aaagccgaaa atgatatgaa gaagaaaata gaaaaagttt
     7021 tgagagagga gaatgcaaac tcattagaat taaaaggaaa aataacgttt aaaaaatttt
     7081 acgaatctaa atggcttcct agatatgaat taggtcaaac aattagatca aacaggcctc
     7141 ctagtgatat aaccattagt aatacaaaag atatatttcg gttgcatatt ttacctatgt
     7201 ttggtgaata cgcaatgaat tatctaaata tcaatactga gataatatca gatgaattaa
     7261 ccaaaaaatc gaaagaatat gcaaatataa aaatcataaa aggatatgtt cgttccatgt
     7321 tcgatattgc agaaatacta aattatatcg aatttaatcg aactactaaa attattcaaa
     7381 gtatcactgc acctaaaaaa aatgctttag aagaaaaaag aatacaagaa ggtaagcaag
     7441 cactatcatc aaaagaactt actaattgga tagaagcagt aaatgatgat tttaataatc
     7501 atttacttac ttttcatgat tacacacttt ttatgattac cctgtatctt ggtgatagaa
     7561 aaagtgagac ttacgctttg caatggaaat atattgattt tgaaaaacaa acggtccgtc
     7621 ttaaacatac tttagataaa tatcaaagaa aaaagtttac aaaaggtcga aaagataccg
     7681 tcattcaagt accagaagtt gtaatgacat tgctaagtga atggaaatcg gtacaagctg
     7741 atcaattgtt gaaattaaaa attaagcaaa cgttagatca gtatctcttt acttatacta
     7801 aaccatctgg agaagtaaat tgtcctgttc atgccgatta cctaaactat cgaatcaatt
     7861 caattaaaag aaggcatcct gacttagttc atttatctcc acacaaactc cgtcatactt
     7921 atgcaactat agctagacaa ggtggagcag atatgaatca aatttcaaat gctttaacac
     7981 actcagatat ttctacaaca aagatatatg tgaacacacc agatatagtt gataaggctg
     8041 tttttgaagc ttttcagaga ggattgaata agtgtgattg atatgtgttc ttaaatgtgt
     8101 tcttaaaaac aaaagaagca ctttccaaat aattgaaaag tgccagaaac gttgatatat
     8161 aaaggatatt aacgttttga gaattgtgaa gctttacgtg ctttcttaag acctggtttt
     8221 ttacgttcga ccatacgtgc gtcacgagta agtaagccag cacgtttaag agctaaacgg
     8281 aagtcaggat caacttctag caatgcacga gcgataccat gacggatagc tcctgattgt
     8341 ccagcgtatc ctccgccgtt tacgttaaca aatacgtcat atgcgccttt tgtttcagta
     8401 acagcgaaag gttgattgat tacttcacgc aagtcagcat gtggaatgta ttcttcaaca
     8461 tcttttttat ttacagtaat tttgccagtt cctggtacta gacgtacgcg agcaactgcg
     8521 tttttacgac ggcctgtgcc gatatattga acttgtgcca atgaaattcc ctcctattag
     8581 attaagtttg tgatgtctaa aacttctggt tgttgtgctg catgtggatg ttctgctcca
     8641 ccgtatacgt tcaattttgt aaattgtttg cggcctaaag tgtttttagg aagcatacct
     8701 tttacagaag tttcgatcaa acgacgagaa tttttagcac gcaattcgcc agcagtgatt
     8761 gatttcaatc ctcctgggta gttgctgtga cggtaataaa ttttgtcagt cgctttttta
     8821 cctgttaatt tcactttgtc tgcattgatg acgatgacga aatcgccagt atccaaatga
     8881 ggtgtgaaag ttggtttatt tttaccacgt agtacagatg caacaactgt tgaaagacgt
     8941 cccaatggaa catcagttgc gtctactaca taccatttac gttctacttc gcctggtttg
     9001 gccatatatg ttgtacgcac gttattttcc tccaatttaa gttctgatgt ttgacataca
     9061 cttcgagttt ccggggctct ttgtggggca aacaatacca tttaacatat tacccgctaa
     9121 tctagtcaat gtcaacgttt ttctgcttga ttgaaaaatt tatatgaaac tttacttctt
     9181 ttctttcttt tatataaaag ggtcgtttcc tgtcaaaatg aaaaacaaaa agaggctgtg
     9241 acaaaagtct tgtcacaacc ccttattttg aataaacggt gttctcctgc caactcctcg
     9301 gtcacaagtc acaatatcca taaaacataa gaagctgttt tttaaaattg tttcttgtga
     9361 ctcggagctg aacggcaggc ttacaacctc ttggtaaacg gtgttcaatc agctgaccct
     9421 cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgacgtttg tttctctaat
     9481 actcaggtca aaacgctgat ttcacaacct ctttttcggt caagttcaac aacagttttt
     9541 acgcgtcgat ctctggcgct ttgcccaaat gagcttggat catccagata cgtttttctg
     9601 tggctgtttt gaattcgata aagatatctt gtgttggatc gtcgccttct tctccagaaa
     9661 cttcgattcc tttttggtaa agatctgcta gataacgata gcctgcaaca agtgtttcca
     9721 aacgttctgg aatagtacga tcccagcggc caatttcatc agggattttt gtgttatctg
     9781 caaattcacg caatgtagaa taaggtgaac cgtctaatgt gatcaagcgt tcggatactt
     9841 catctaactg agcgttcaaa tcatccatga actcatccat cattgggtgc aatgtaagga
     9901 aacctggtcc tctcatgtac caatgtgttt ggtgaacaac aacagacatt tggcttagat
     9961 ctgcaactaa ttgatttaat acttctttgg tttgttggaa tttcatcttt cttgcctcct
    10021 aacattttct tacacttaaa ataatagtag actttcaatg aaaagtacaa caaaatcatt
    10081 tacctgagaa cgattttcag ttcgcttttt tatccgtaaa gccaatcatt tttcagaatc
    10141 agctaattct tctcgcagtt gagcaaattg ctgataagtc tgtgctaaat gcttttgtgt
    10201 cgaaacaaat aatggataga ttttctgata ttttttatga tcagtttctt gtactccaac
    10261 ttgctctttc aattctatca attcctgtgc ttcactgata ctttggatct ttccgatgct
    10321 tctccagcct aaaatcacag ctcctaatgc ggatgcttct gtgacagtag ggaaaacaag
    10381 cgggtaaccc agtacatcag ccatcatctg acgaaaaatc ggagattgag caaacccgcc
    10441 tgtagcacgg atctctgtga gctgattttc ctggcaaact ccttttaaaa tccgataaag
    10501 gttgagacag atcccttcta gaatcgcctt catcatttct ttttctgtat gttgccgctg
    10561 taaaccgata aaactgccaa acgcatctgc gtcccaaagc ggtgctcgct caccaagtaa
    10621 atagggatga aaaatcagac cattggcacc cgctggcaca aaagcgatag ccgaaagcag
    10681 tgcatcatat ggattttctc ctttttcttt agcagattga acgacttcct gtaaaaatgt
    10741 atgccctgcc caatcaaaga tgccagcacc attgctagtc gctcctccca ctacccaatg
    10801 tttctgatcc agaacataac aaaacgtctc catttctgga tggaactgag gctgagtagt
    10861 aataaaacga agtgctccgc tcgttcctac ggtcaaagcg gcgatcgtat caccaaaagc
    10921 acctaaccct agattagaaa gcggcccgtc tgcccctccc aaaacaaatg atatatcatt
    10981 tggcaaacct agttctgctg ctgtttttga tggtagacct gttacttgat aggtggaagg
    11041 aaagacttgc ggcaattgct ctattcgaat gttcaagtag tcaagcactt cttctgccca
    11101 ttggaattca tggatatcaa aatatccagt tcctgaagct gttccgtagt cgctgatcag
    11161 ttcgccaaac aatctataaa agatatagtc tttgataccg acaaaataag cagttttttt
    11221 gaataattca ggttggctgt tttgcagcca cttgattttg ttcaatggtg tcattggatg
    11281 aatcggcgtc ccagtctgcc gataaaattc tttgccaagc acggtatatt tcagtttttc
    11341 tgcttcgtcc gctgctcggt tatctgccca agtgatcatt cgagataacg gtgttttctc
    11401 ttcatccaac aatatcaggc catgcatcgc agaggagaag gagacaaatt gaatgattgt
    11461 gtctgaaggc aactgtttaa ttacttcttt tataacggtg cagacacctt gaaagatcaa
    11521 ttcctgatct tgttctgcca tcccttgtgt ttcttggatc aaagggtagc gaaaataagc
    11581 ggatgctgct tcttgggctt catctgtgaa agcaactgct ttggtttgtg tggttccgat
    11641 atctaatccg attgaaacgt tcattttttc acctcaaatt ttatgtatgt acaaaaattt
    11701 agtttaagct tgcgtatctt ggttttgaaa agggggagat ggcgtgttac tttattttat
    11761 cattggttgc tgtatcggat catttttatg tttaaccgcc cagcggattc cgttaggtca
    11821 ctctattatt tatcctagat ctcattgtgt caaatgctgt cgttcgcttt cttggtatga
    11881 attgattccg attctctcga tcatcgtcca acgatttcgc tgccgctatt gcaggtgtcg
    11941 gttgcccttc tactatttgt tggcagaagc cttatgtggt ggtctttttg cctggttctt
    12001 tactttttct tccgcaagaa atttttcgac gttcatatgg atgctttcgg ctttgctatt
    12061 ttcgttgatg gatttatttt acttcatggt ccattctcac acgctattct gttcttgggc
    12121 aattttatgg attttttggt tgcaggccgg cgtcttccaa tggcaaagtg tgctgttaac
    12181 gtttatcgta ggcggtgtgt gccttcgcta tggtcaatcc tatctcggag ctggcgatac
    12241 tctgttgatg ctttcttgga gtggtggctt gtctctagaa gagttgcttc agatcttgtt
    12301 tcttgctagt ctgtttggaa tatccgtttt ttctgtttac tttatctgtc ataaaaaaaa
    12361 gttggaaaag ctgcctttca ttcctttttt gagcagtgcg ttgctgattg tccttcatct
    12421 taggtattga aaaaaggttg tgacaaacga cttgtcacaa cctctgatat atagtgttct
    12481 cttaccaaat ccttgtgact tggagctagg cgacaggttc acaacctctc cactaaatgg
    12541 tgttcaatca gctgaccctc agtattagct caaaatgctg gttttacaac ctctgatata
    12601 cggtgttcca taagtagctt tttcggcttc ttatttcttg acgccccaat gtcgacgaag
    12661 gaaacctgca cgtccagaac cttctcgata aagattgtaa tggagcttgt ttttcttgta
    12721 ataatcctga tggtaatctt ctgcaggata aaattcgctt cttggttcga tttttgtcac
    12781 gattggctta tcgaatcgcc cgctgtcttg caatgcttgt tttgaacgct ctgcaatatt
    12841 tcgttcttct tcatcattat agtagatcac tggacgataa gaatcaccgc gatcagcgaa
    12901 ctgtcctaat gcgtcagtcg gatcggtttg ctgccaatag atttccacca actgatcata
    12961 agaaataact tctggatcat attcgatctc tactgcttct gtatgccctg ttgtgcctgt
    13021 tgttacttgt tcataagttg gatttggtac atgcccgcca gtataaccgg atgtcacaga
    13081 aatgatccct ggctgctctt caaatggttt gaccatacac caaaagcatc cgcctgcgaa
    13141 aactgctttt tttgtcatat aaaatccccc ttttttctca tcataccttg atttccaaaa
    13201 ggaggataac gttttgcttg tgacagaaaa aacggaaact attatatacg tgatacggct
    13261 tctgataaat gccaaaacga acgtacttga tcaaaaacgg ttaaatcagg aatcacatat
    13321 tcagcagaag gaagatttat gttttcacct attcccaata catgcatgtt accagccaaa
    13381 gctgcttgac aaccagcttc tgaatcttca atgaccaagc atgcatttgg cggtacattc
    13441 aattgctgtg ctcctttgag aaaaacttct ggatcaggtt ttgcttttga tacttgcgtg
    13501 ccgtcaataa ggacatcaaa ataacttgtc aaattcgttt tttctaaaat gaggcgtgca
    13561 tttttgcttg ctgatcccaa tccgatcttg atgtttgttt ttttcaagta ctcaagaaca
    13621 gctattgcac caggcaaaac ggatgtttca tccatttttt gaatcgcttg aagatataga
    13681 ttgttttttt gctcagcaaa ggctgatttc tctttttctg agaatttctg ttccattttt
    13741 ccaaatgaca gcaatcgctc tagtgactcg gttctggata ttcctttcag ttgctcgttt
    13801 tctttctcag tgaatggaat ggacagctgg ttagctaaaa ccagccacgc ttcgtaatga
    13861 taacgagctg tgtctacaag aacgccatct aaatcgaata gtgctgcttt gattttcatc
    13921 gattacctcc ttgaaaatga acgacttgaa gtaaagatgc gatgttttta gctgtgcgat
    13981 gaagattatc agtggcttgc tgtaaagctt cttgtaaagg cacagctgca ggaaggatcg
    14041 gaaaaacggc cgaaatgcca acatctgctg ctttttcgct tcctttacca acacttccgc
    14101 aaatagctat cacaggaacg ccctctggta ctcttctagc aatcccaact ggcgcttttc
    14161 cttttagact ttgtccatct agacgtcctt cgcctacgat tacgaggtct gcttgctgta
    14221 cccgtttatc gaactccagt aaatccaata cgtagtcgat gccagagact attttccctg
    14281 aagcaaatgc taacagtcct gcacccattc caccaccagc actactgccc gatgcctcga
    14341 ttacttgagg ggcaaatctc atataaaaat catgcatggc ttggtcagcc actgctaatt
    14401 cattttcttt caagcctttt tgtggtccga aaacaaaaga agcgccttct tttccgcata
    14461 aaagattagt aacatcagta atgacagtga ttttggcttt aatcagctca ataggcacgt
    14521 tcgttttatc aactgtagca attttaccta gattttctcc cagtgcttct actttttgat
    14581 tatcttgatc gtaaaaagca taacctagac cttctgccat gccgatgccg ccatcgttgg
    14641 tactacttcc gccaacgccg atgatgatgt gtcgaacacc ttgttgcaac aattgtcgta
    14701 tcagttctcc tactcctcgc gtggataacc taagtggctg ttgttggttt agtgggacat
    14761 ctttcaatcc gacgatttct gccatttcaa aaatcgcagt ttgaccgtct gaagcgtact
    14821 ggatttcttt gtttttgcca aatgcattag taacgactgc tgtttgccgc ttcaacctta
    14881 aactgtctgc taatgatgaa accgttcctt ctcctccatc gcctataggt agcagatcat
    14941 attcagcctg aacaaaaact tcttgaaaac ctgattgaat ggcttgtgct gcctctaagg
    15001 ctgtcagact ttctttgaaa gaatctggcg caatcagaat acgcattatt ttcccctccg
    15061 ttccaagatc agttcttttt ccagctggta ttggtctcca ttgacaaaaa taggtaatgt
    15121 ttctttcgtc tcttttttta ttactatttg tttttttgtc aaaacaaata gcaacctgtg
    15181 atttttccaa ataaaaggaa aagaaatttt ttcccatgct gaaggaagtc ttggagaaat
    15241 agaaagagtt ccattcgtta ctgtaatacc tgaaaaacca aatataactg ctagccatag
    15301 tgctcctaat gaagcagcat gaattccgtc atctgatgaa tgcggatctg gtcctaaatc
    15361 aatcaggcac gcttcttgga ataaatgata cgcccattcc atttcttgga tacggcaagc
    15421 ctctatggcg tgaatcgctt tactcaacga tgagtcgtgg attgttttgt tttcatagta
    15481 atgcaaattc ttagaaacga ttggttgaga aaaatactca ggtaataagt ataacagcat
    15541 gacaagatct gcttgcttgc tgacttggag ttcatttact tcgctacggg cataatcaag
    15601 taaaatagac tggctgccag gacgttcttt gtaagtggtc aaatcaataa ctggcttact
    15661 gagatacgtg tcatcttgcg gaatcagctg ttctttgtta ggcactggca aatataactt
    15721 atctaaaaaa ttttgacatt ctttcaaaaa aagcggatcg ccagcttgat atttaacagc
    15781 taaggccaca ttataatgcg ccagatagtt cgtatatgca ttattgtcga tatattctgt
    15841 gtattcatct ggaccaatga ctctgtgtat ctctaaatga tcatcgactt ttacagctct
    15901 gctcagccaa aaagctgctg tttcatgaat gagcgttttt ccctttgttt ctaaaaacga
    15961 ttgatcctct gttgcttgga catactcttg aacggcataa gcaaggtctg cactgatatg
    16021 atgttctgcg gcagctgaag caacttcttg tcttgttccg gttttgatat tcaatgcagc
    16081 aaatttgggt gtctcttcct gaccagtgtg tgcgctttcc catgggaaaa gtgcaccttg
    16141 atagtgataa tctcttgctt tttgccgtgc gccttctaat cgatcataac ggtacagcag
    16201 caaattcttt gctacttcag gaaactgata taaaaagaaa ggcaggataa aaatttccgt
    16261 atcccagaaa acatgccctt tataaccttc gccagttagt cccttcgccc ctacactaac
    16321 acttggatca ttgcggggag tcataatctg taaatggtaa gcagcgaagt ccatcgctaa
    16381 ctgatcgaaa gaagaatcgc cttggatcgt tatccgatgc tgatgccaaa aatctgtcca
    16441 ataagacatc gactgctcta aaatttctgt ataactaacg cttttctggg cttgggcaat
    16501 acttttctta aacgggtctg tttgctgaag agaggtagag agcacgatga tcttctccag
    16561 cgtaaaggac gtattttttc tgatcttttc tgaaaaggaa gcggtgatcc ggcgattttt
    16621 tgcttggaat acacctgctt tattgaattt agctgcaatc ccaatatcaa ttttactttc
    16681 tgttgtctgg taggatgcac tgatcaaatc gtcaccaaat actcgcagtt ctttttcaac
    16741 caactgctgt gtaccaaaat tggtttgctg tccatcgata cccgttttca catctacttg
    16801 gcagtcttcg gtcaacggct tgatgaccac tttcagggct gcctgatgga gattattttg
    16861 atcagcaaca cgacaagtaa tgacttccac tttttttcct tgagcagttt caatgagcgc
    16921 atagcgttta agctctccca tttctaaatc tagtgcccgc tcgtatgcaa gaactttcat
    16981 cgtctcaaga gaaaaatgtt caccgtcgat ttttatttcc catctgatga catctgggag
    17041 attcactaac tcggctgatt ctgctcctaa aggtcggtta tatacaccag ctgcaaacat
    17101 tccccttact tgattccgat agtcttcctc ctggcttcct ctgataccaa aaaatccatt
    17161 tccaatggtc atctgactcg catacttatt gatatactct aaatcaaact gtttttcttc
    17221 tactttcata aggtaataac ttctcctgtt tctgccgaac gatataaatt ttccaccaga
    17281 ttctggatga catatccttg ccagccatct gcaatcatct gagtctggtc accgagcgcc
    17341 cgattcacaa aatgagccat acttagttca tgtgggtcat taactggcgt ggtcttttgt
    17401 aaggaaacca gctttccatc tacatcttcg aatagttcgc ctggatacag attagcacct
    17461 gcctgatcgc cacaaaaagc cacattcagt ttgtcttctc tgatattcaa agcaaacgaa
    17521 gtattcagtt ggatgatctt gccgttttct aattccagtg tgccaaaaat tgcatcttct
    17581 actgtgaatt tgcttggatc ccatttcccg aatttccctg aatttttttt cgtaccaatt
    17641 ttttggaagc tgtacgcccg aacgcttttt actttaggaa atccagtaat atacatcgct
    17701 gtatcaagca tgtgaatgcc ataatcgatc aaaggcccgc ctccttgaag ttctttgtcg
    17761 ataaacgttc cccagcctgg aacaccagac cgacgaagcg cattcgcttc tacgtaataa
    17821 atatccccga gaacatcaag atgattcttg atcattgttg cttcgtcaga aaaacgatgc
    17881 tggaatccat aagccaaaat ctgcccgttt ttatcggctg tctctgccat ctttcttgct
    17941 tgatcagctg tcatagctgg tggtttctcg cacatcactg aagcattagc ttcgagggca
    18001 gccaatacat gttcgaagtg aaagcgattg ggtgtacaga cactcacgat atcgggagaa
    18061 atctcattca gcatttgtgt aactgatgta tacgcatgag aaaatcctgt acgtcggcaa
    18121 aagtcttctg ctcgctggct gactggatca accacagcgg ccaattctaa gacatctgat
    18181 cttttttgat aataagcagc atgtactttt tctgctactt gtccagcgcc tatgattgct
    18241 actttcttca tttatttctc cctcctttgc ttatttactt attgttattc tttctctatt
    18301 ctttctctaa atcatttaaa tgacgcgctg tttccagaca gtttttcaag tattgcaatg
    18361 attcctgata aattttcact tcattttctc ctcgcaaacg acattcatat accatataac
    18421 cttcatactc attttgaagt agcgtttgaa aatggcgagt aaaatcaatt gcgccagatc
    18481 ctggttggta gcgatgatta tctgctaaat ggatatgacc cacatactct ttttgcgtag
    18541 tcaatgcttt tgaaatatca tcttcttcaa tattcatatg ataaaaatca gcgatgatct
    18601 gcacatgttt caattgatta gagtcaatgt aatgacgcgc atccgctaac aaattgatca
    18661 tatggtcttg gtatcggttc aaaggctcca aataaataac cgtacctgtt tcttcggcta
    18721 ctttatctaa ataacgaagt gattcgctga ctgctttata gtctccttct ttgcttcggg
    18781 gtgaaaccat cggcggtaat cgataagtga acattcccca agctgctggg ataacgattc
    18841 ctttgcctcc aacctcgctt agtgcacgca aaatctcagc aatttgacgt acaccattaa
    18901 gacgccgttc ttcgataaag tcaccgatcc acccatcgta gccgttacaa gctgtcacta
    18961 ctgggatccc tgtttctttg attgcctgtt tgactgtgtc gatattttct attaacaact
    19021 taccgtcgat ttcgtattct tcaaatccac agtctttgat aaatctaaat ttttctaaaa
    19081 tgtctcttgg gaaaaatgct tgatcttgcg taccgaattt cattgttctc ctcctcggac
    19141 ttgaacgccc attttgatac tttcttctgg gtgctggtct acataacgca tgtaagcttc
    19201 tgcgctttct gcaaatggca caactggatc aatgatttct tcacaatcca agtaaccgtt
    19261 catcaataat tcccaacatg tgtcctcgat tcgtttacgg ttccatcttg ggtaatctgg
    19321 gtttggttca ctagatgctc gagcaaagac gatttttgca ttattgaaat gtgcttcgcg
    19381 accaagatta aacccagctg gaaaaggttt agcaaaggca acataagaaa tgatcccgcc
    19441 atagccgatc cctcttaaag aagcttgtaa tgcggcagca ctgccactcg tttcaataac
    19501 tgaatctact ccacgtttat ctgtcaattc ctttagacga aaaccgacat ctgctttcat
    19561 aggatctagc gtgtaatcgg caccgtattt ttctgctaat tctctacgct gcgctatggg
    19621 atcgatccca acgatggtag tagcacctgc ttttttagct aattgaatcg caatttgccc
    19681 gatagcgcct aaaccgaaga ttgctacagc atcacctgct cgaacatgtg cttcccggat
    19741 accacctaaa gcgaattgag caggatcgta acatactgca tttttccatt gatccaaccg
    19801 atcaagttta cgtagtttat aattatcaac accgtttgta atcagcgttt cacgaatggg
    19861 tccataagtg cagactaact ctcctttttg atactctgtc accaaactgc ccgtttcttc
    19921 gatcgtaccg acaatcatat ttcctaattg aaattcgcca aataccacac cgcgttggct
    19981 tccttcttct cttggcaaga ataggcgcca ttcttctgaa aattcttcat cgataaatgg
    20041 acttaatcct ctaaagtcga caatttctgt tcctggtttt ggagtagcga aaattactcg
    20101 gatacgtact tcgttctttt cgatctcacg gtcttcgtaa tctttgaatc cggctattct
    20161 tggttctaat gcaactaatt ttttcatctt ttctttcctc tttcttttct ttttatcctt
    20221 ttacgccgcc ttcgactaaa tcacctttaa taaatcgttc agataacgca tacataatga
    20281 tgactggtaa agcggtcacc aatgaagcgg ccatcattcg tccccaaatg tagtctggtg
    20341 tactgaataa tgtgttcaaa ccgattggta aagtaaattt ttcggaggtc gacaggaaaa
    20401 tcgaagcaaa taaataatca ttccacgaaa ccataaagca ataaacgaag acagaaatga
    20461 ttccagaaat agccaatggt ataatgatcc gaataatgat ctggatccgg ctaagaccat
    20521 cgatcattgc tgcttcttca atatcagtcg gaatcgtgtc aaaataactt tttagcatga
    20581 atatcgcagt aggaagtgtt tgaacgacca tcgtgataat cagtgctgta ttggtatcat
    20641 acaaacctag accggaaatg attttaaata atggaacaac gatcaaaata ccggaaaaca
    20701 tataaacggt gtaaaagcta gcattgattt tcgagcgagc tttgaagcgt agttttgaca
    20761 aagcgtatgc cccgctgatc ccaatcacta ctgcagttac ggaggctatc aaggagatca
    20821 ctaaactatt tttgaaatac atgatgaatg gaaaaatctc agcattgaaa atatcctgat
    20881 aatgttcaaa agtccattct ttcggaaaaa tagtttgtgt cgttgcaatt gcttcttgtg
    20941 aatccttgaa tgatgtcatc agcatgatca aaaatggaaa caaattgaca gctaatagca
    21001 acaaaatcgt cgcgtaaaat aagattttcc tgctcttttt atttttcatt tcaagtccac
    21061 cttctttcgt gagagcaaga cgaccgctgc aataatgatg aacaatagaa tcgatatagc
    21121 tgctgctttt ccaagatcat tgaacgcaaa ggctgtctga tacaaataaa cgcctaagat
    21181 attgacttta ttcgtcagta gatagacatc tgtaaacata tagaacatcc agatagatcg
    21241 taaaacaatc acagttgcta gtactggttt gattgccggc agtgtcacga tcttgaactt
    21301 ctgccaagca tttgcaccgt ccatttcagc tgcttcataa agcgttttgt cgactgtttg
    21361 caaaatagcc aaaaatgaaa taaacgcata cggatagtat ctccagatag caaacaaaac
    21421 gaccataaag aagctagcaa ctggctggtc gaaccacagc ggtgcttcgt tgaaaagctg
    21481 caacctatct actaacagat cattgacgat tccataacta ttattgaaca tatatttcca
    21541 agcaaaaatc aaagaaatag atggcgtaac ataacttaat aaaatcagtg aacgcaccgt
    21601 tttcctaaat ctgaacttgc ggttaagcaa aatagctacg gctaatccta aagctgtact
    21661 gcctgctaca actaagaatg tgtaagcaac tgttaaacct aaagaacgat aaaattcact
    21721 atcacttaaa atttcaagat aattgcttaa tccgataaaa acggcgtctg agttaggatt
    21781 cagcggcata tcgaaaaaac tgatctgaat attggaaagc attggaatca gcactaaaac
    21841 agctaataaa agcatgcttg gtgctaaaag tcccatggct aattttaagt cagaatgttt
    21901 ccttggacga ttcattgaaa cctccccctg ataatctgcc aatagctatc tacttttttc
    21961 gaacaccatt ggcagaactt cttgttattt cgtatttttt tgtgcgtcag ctaaagcttg
    22021 atcgacatct tcgtttccga cagttacttg gttcactgct tttccgatca cacctgagct
    22081 tgtgatatct cccattttcg tgaaattctt ttccccgact agtccaaaga cttgtacttc
    22141 atcaaaagca ctagcaatct cagatggcag ctcgccaaaa gacttgatga cttcattttc
    22201 tttgtaacca tccagttcga ctacttgttt attcactggt tgagcaccac caggagacat
    22261 caagacccac ttttccatgt ttttcggttg agataagaaa gctgtgaatg cttcagcagc
    22321 ctctttttgt ttctcgtcta gaccagaaga aatcgtcaat ccagatactg tgccatagac
    22381 agcttgatct ttttgtgttg ggatagcaaa accgatattt tctgagttcc ccgcttcata
    22441 aacagaaggc aaaatgtaag ttgaatagat tgccattggt gctgttccat tcatgaaagc
    22501 atctttgatt tctgtcacat cattagagcc tggcatcgtg tattgggata agttttgata
    22561 aaacgacaat gcttctctca tcttttccgt atcgatcgtt acctcgcctt catcatccaa
    22621 aacatttgct ccatttgaca aagcaaattg agagaacgct tgctcactca ttgttccttc
    22681 agcagtaggc atagcaattc catattgttt attctcttta tctgtgaaat gttgagcaat
    22741 ttttaaaata tcatcccaat tttctggttc tgaaaaacca gcttctgata atttttcttt
    22801 gtcataccaa ataccttgaa cccagccgct aattggtgca gcaatatagc tttttccatc
    22861 ttctgaacga acaaggtttt tggctccgtc ataataatta tcttctccag cttcctcgat
    22921 aacgttttgg actgccgtct gatcaatcag ttcatcttta tccatcactt tagcaaaatc
    22981 ctgactgact tccattactt ctggtaattt accggaacga gctaacgtaa cgatttttgt
    23041 attgaacgct gattcttcta ccggcacttg cttgattttg atgtctggat tttctttttc
    23101 aaaatcagca accagttcat tgatcacact taatcgttcc tgttcgacag aagagtgcat
    23161 gaattgaatc gttgtctgtt gtgattcgtt agagctttga ctttcaccac aagcagtaag
    23221 aagaaatcca gtagctagca gacaggtagt aacaataatt tttttcattt ttttccacct
    23281 cttattttat tattttcagc cacatatatt catacggctg gaatatcaac tttcgttctt
    23341 gactgcaatg gataatttgt ccagaaaaca ggtctaggta atctcctttt ggtaaaagat
    23401 aacctgctga ttgattggaa aaattatgga taactaagac tttctcttcc ttcgcttcgc
    23461 gaataaaact gataaaagaa tctgctgagt ccagtaagta aatcaaacta ttcggatgga
    23521 aggctttttc tttttttcga atagatagca tctgggtcaa ttcaccatat actgctgttc
    23581 ttaatggatc tgtctgcaac tctttctcaa tatctgctaa caaaaatttt tgccgattga
    23641 tttttcgatt ctctcctgtt ttttctacgc ctttgtaatc atttcgactt cccagcacac
    23701 tttgaatata aattgccggc acgcctggta acgttaccaa aaatgaatga gcagctaaaa
    23761 agcgtttgct tctccattcc gtcgtatctg ttggagaact taacgcatct agatacgtag
    23821 cgtttatctc atatggactg gtcgttccat ctgggttttt cttgtagttt actcgggtac
    23881 cctctttctt cagctgttcc actaattcaa ggatcgtttt ttccggcaga atacctcgta
    23941 ccggattcaa tccgataccg tcatgggagg ctaaaaaatt aaaaaaagtt gtttttttcg
    24001 atgcgaaatc gatggttttt aaccattctt tcaacttact tgtattaccc gaacgaatcg
    24061 cttgaagggt caaaggtggt agcgaaaact gatagaccat ctgtgcttca ttctcgccat
    24121 tcccaaaata actgatatta tctttgtgtg gcacatttgt ctcagtgatc aataccgttc
    24181 ctggagctgc ttcatcagcg attgcacgga ataattggat gattttatgc gtttctggca
    24241 ggtgtataca agaagttcct agttctttcc ataaaaatcc cacagcgtcc aagcgaataa
    24301 atgaagcgcc tttttctaag taaaacaaaa gaatatcgat cattttcaat aaaacttctg
    24361 gatttttaaa attcaagtct acttgatctt cgctaaaagt cgtccatacg tgtttttctt
    24421 tcccgttttt gtcaatgaac gttgtaagta acgggctggt ccttggtcta gtcacgctgc
    24481 ttgtatctgt atccagtggc aaatcaataa aaaaatcttt gtacgtatca ttcaagttta
    24541 aatattcctg aaaccaagta ctttcggcag acatatgatt gcagacaaag tcgaacatta
    24601 accgtgtttc gcttctcatt tcttctatat cttcccatgt gcccaaacgg tcgttgattg
    24661 cctgataatt tacaacggag aaaccatcat cagatgtata tgggaaaaat ggcaagaaat
    24721 gtacaactct aaatgtatct gccaaatact tttgataaaa tttcttaaat gtacgcaaag
    24781 ttggttgctg ctcttcaaaa aattgatccc cataagtaat caagaaaata tcttcttcgt
    24841 cccaatgcag tttgcctgcc tggcctttct tctgctgata aacactgact ttttgttcaa
    24901 tcatagaggc ggttttctca gacaaatctg agtcgcgata aatcgcttgt aaaagttttg
    24961 tatacatcgg attccccttt cttttttggg agcgttccca acgatttgat catattatgg
    25021 aaaacgctta ctgtcaatag cattttttta aataattttg atatacacaa aaatacaaat
    25081 aagaaagctt atgtatcaat aattaatagc ttcttaaaaa aatatggtct ctcatgatca
    25141 tgcaagctat tcctaaattt tcaaaatcat agacaaaaaa gaatgtctat tgtactatgg
    25201 aattattcta aaacgctccc aaaaaggagg tgcatataaa attgaaacct actatttatg
    25261 acattgcgaa aaaagctaat gtctcaaaat caactgtctc cagagtatta aataaccagc
    25321 caaatatctc tgataaagca aaagcaagtg tacttcaagc aatcaaagaa ttgaattatg
    25381 aaccgagcaa aattgcaaga ggactgtcag gaaatggttt tgacgcgatt ctagcattat
    25441 ctcatcgtac ttctcatacg acctatggca atcctttttt ttcagatgtt attcaatcca
    25501 tttctgccgt ttctgaacaa aataacttcg acttgattct acaaacggca aaaacaccag
    25561 aagaagagct agaaaaatgc gcgaaaaaga tccaagaaaa aatgatccga ggaattatta
    25621 ttttaagctc tacgatggat gaaacacttc tttttgaatt ggatcaatac aatattccta
    25681 ttgtggtaat cggaaaagtc ggaaacgact acacgaacat ctactcagtg gacacggata
    25741 atttccagga tagttacgat ttggtggaaa cactgatcaa gaatggacac cgtcatatcg
    25801 gctgtctcca tgctccgaca gaagtttatg tatccaatga tcgtctcaat ggttaccgtc
    25861 aatgcttatt tgatcaccac ctagatatcc gtaatgaatg gattatagac ggcggctttc
    25921 gcttagaaga tagcatggca gcaatgaatg atttaatgaa gctacgagaa agaccaactg
    25981 cgattttttc taccgatgca ttaaaatcat tgagtattta caaagtggca gaacaatctg
    26041 gaaagcagat acctgatgat ctttcgatcg caggattcag tgatccctta ttgtcctccc
    26101 tattttcgcc acagttgact cggatagaca ttccgacaaa tcagttagga atcaaagcca
    26161 cagaactttt attccaacta atccaccacc aataccctga agaaaaaaga atcattcttt
    26221 caaccgagat tactctcaca gattcgatcc aacaacaaga aatgtagaac agaattataa
    26281 aagaacagaa acactgagga taagtcagtt actctgttct tttttcaatc actcatccct
    26341 cgttaccccc tcccggtatt ttagcgaatt tttcttattt tccatctgtt ttagatagct
    26401 gtggataact ataatgtttg tgttaaacta ttttttccac agggaagaaa aaactatttt
    26461 taaccacgtt gtgtataagt ttttatagtt cgttttttta aacacattta cccttatttc
    26521 aatccatgtt atttggggat acttatctct aaaacataaa tagaataaca gacatgtcta
    26581 ctggttttgt tttattcgag gaattattca ataatagcta gcaaaatatt aaaaataacc
    26641 gctctatctt cccatcaatt tattttgcat tcaaaaaatg atctatcttt acttttgttg
    26701 attgacttct cttggaagat gatcgttccg ataccttaat tggatacgaa ccgatgttgt
    26761 taagatcctt tcccactttt cttctagtta gacaaattat tttagac
//
LOCUS       DAZAID010000031        26663 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000031 DAZAID010000000
VERSION     DAZAID010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.031"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..888)
                     /locus_tag="ACG9BE_001219"
     CDS             complement(<1..888)
                     /locus_tag="ACG9BE_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3031976.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKG"
     gene            complement(1017..1781)
                     /locus_tag="ACG9BE_001220"
     CDS             complement(1017..1781)
                     /locus_tag="ACG9BE_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN3031977.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            complement(1785..2174)
                     /locus_tag="ACG9BE_001221"
     CDS             complement(1785..2174)
                     /locus_tag="ACG9BE_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031978.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            2309..2851
                     /locus_tag="ACG9BE_001222"
     CDS             2309..2851
                     /locus_tag="ACG9BE_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFN3031979.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            2999..3631
                     /locus_tag="ACG9BE_001223"
     CDS             2999..3631
                     /locus_tag="ACG9BE_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3031980.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(3765..3890)
                     /locus_tag="ACG9BE_001224"
     CDS             complement(3765..3890)
                     /locus_tag="ACG9BE_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFN3031981.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(4024..4917)
                     /locus_tag="ACG9BE_001225"
     CDS             complement(4024..4917)
                     /locus_tag="ACG9BE_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFN3031982.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(5099..6025)
                     /locus_tag="ACG9BE_001226"
     CDS             complement(5099..6025)
                     /locus_tag="ACG9BE_001226"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFN3031983.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(6110..6871)
                     /gene="pflA"
                     /locus_tag="ACG9BE_001227"
     CDS             complement(6110..6871)
                     /gene="pflA"
                     /locus_tag="ACG9BE_001227"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFN3031984.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(7115..9346)
                     /gene="pflB"
                     /locus_tag="ACG9BE_001228"
     CDS             complement(7115..9346)
                     /gene="pflB"
                     /locus_tag="ACG9BE_001228"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFN3031985.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(9623..12073)
                     /gene="parC"
                     /locus_tag="ACG9BE_001229"
     CDS             complement(9623..12073)
                     /gene="parC"
                     /locus_tag="ACG9BE_001229"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFN3031986.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(12085..14130)
                     /gene="parE"
                     /locus_tag="ACG9BE_001230"
     CDS             complement(12085..14130)
                     /gene="parE"
                     /locus_tag="ACG9BE_001230"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFN3031987.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(14296..14730)
                     /locus_tag="ACG9BE_001231"
     CDS             complement(14296..14730)
                     /locus_tag="ACG9BE_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFN3031988.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            14857..15495
                     /gene="plsY"
                     /locus_tag="ACG9BE_001232"
     CDS             14857..15495
                     /gene="plsY"
                     /locus_tag="ACG9BE_001232"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFN3031989.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(15627..16502)
                     /locus_tag="ACG9BE_001233"
     CDS             complement(15627..16502)
                     /locus_tag="ACG9BE_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFN3031990.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(16584..17375)
                     /gene="codY"
                     /locus_tag="ACG9BE_001234"
     CDS             complement(16584..17375)
                     /gene="codY"
                     /locus_tag="ACG9BE_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFN3031991.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(17393..18793)
                     /gene="hslU"
                     /locus_tag="ACG9BE_001235"
     CDS             complement(17393..18793)
                     /gene="hslU"
                     /locus_tag="ACG9BE_001235"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFN3031992.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(18807..19355)
                     /gene="hslV"
                     /locus_tag="ACG9BE_001236"
     CDS             complement(18807..19355)
                     /gene="hslV"
                     /locus_tag="ACG9BE_001236"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFN3031993.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(19392..20297)
                     /gene="xerC"
                     /locus_tag="ACG9BE_001237"
     CDS             complement(19392..20297)
                     /gene="xerC"
                     /locus_tag="ACG9BE_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFN3031994.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(20495..21871)
                     /gene="trmFO"
                     /locus_tag="ACG9BE_001238"
     CDS             complement(20495..21871)
                     /gene="trmFO"
                     /locus_tag="ACG9BE_001238"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFN3031995.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(21840..23918)
                     /gene="topA"
                     /locus_tag="ACG9BE_001239"
     CDS             complement(21840..23918)
                     /gene="topA"
                     /locus_tag="ACG9BE_001239"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFN3031996.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(24040..24897)
                     /gene="dprA"
                     /locus_tag="ACG9BE_001240"
     CDS             complement(24040..24897)
                     /gene="dprA"
                     /locus_tag="ACG9BE_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFN3031997.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(24953..25720)
                     /locus_tag="ACG9BE_001241"
     CDS             complement(24953..25720)
                     /locus_tag="ACG9BE_001241"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFN3031998.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(25713..26573)
                     /gene="ylqF"
                     /locus_tag="ACG9BE_001242"
     CDS             complement(25713..26573)
                     /gene="ylqF"
                     /locus_tag="ACG9BE_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFN3031999.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         7074 a         5720 c         4565 g         9304 t
ORIGIN      
        1 ccctttttct tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat
       61 atcttgcttg atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa
      121 tccaatcaca ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc
      181 tcctagtgct agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca
      241 gttttctgtc atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact
      301 aggaagatca acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg
      361 ctcgtccact tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg
      421 cgttgctttt gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt
      481 ttccccaaac aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct
      541 gacacctgtg atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa
      601 ttggagagaa taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta
      661 atagggctca gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt
      721 cgtgacaagt gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa
      781 aactaactct ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt
      841 catttttgaa acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt
      901 tctaacatgt tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat
      961 agcccaaaat aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat
     1021 ttatactttt ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagttgtatt
     1081 tgtaccatat gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta
     1141 tgccgatttt tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt
     1201 ccatgccaaa agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag
     1261 ctcttaaccc atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat
     1321 gcgcgcctta cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg
     1381 cccgctacta tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa
     1441 accccgccaa ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt
     1501 gtgtcaaatt caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa
     1561 gaaaccggct gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc
     1621 tgctgcgcaa tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga
     1681 taatagatga caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact
     1741 ggaatatgat ggacagaacg tttcaatata attggattca attgttactc acctattttc
     1801 attaaatttc ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt
     1861 gtaccaaata atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt
     1921 cccaccactc ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca
     1981 acgctaccga aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa
     2041 ccatctgtca gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa
     2101 tagacaccta agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga
     2161 ttgaccgttt tcattttaat cacctaacct atctttataa ttctattata atcattttat
     2221 catttttttg tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt
     2281 tgttatataa atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt
     2341 cctcattaat tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg
     2401 ctatcgactg attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt
     2461 atcccttgtt ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta
     2521 cgcagtaggt atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat
     2581 ggtcacaacg attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag
     2641 agaacacggt tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt
     2701 tctcgaaata ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat
     2761 cgaagagcgt gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac
     2821 taaaaaagtg agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct
     2881 aaattaaatt ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca
     2941 tatactcatc aatttgttat tatactttat atacttatca atttaatttg gagatattat
     3001 ggaaattcaa cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac
     3061 gatcttattt tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt
     3121 aggctggttg cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt
     3181 gattttgtct tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca
     3241 tcgacatcct ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc
     3301 ttttttagca attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa
     3361 tggtttagtc gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca
     3421 attgaaatgt ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt
     3481 ttcctttttt gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg
     3541 aaatctgttg gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa
     3601 caaacaaaat aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag
     3661 ttttaaacac ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc
     3721 attcgtttct tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct
     3781 tcccataaaa tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt
     3841 cttatcacta cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt
     3901 ccagaccatg tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt
     3961 aaccataaag gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct
     4021 ctcttaggtt ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg
     4081 tataaatttc ttgctcagct cagaaaagat ttgtcgttta agaaaggcca tttcctccat
     4141 tgtcaatgaa ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg
     4201 taattctaat gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg
     4261 gaaaatacta ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc
     4321 tatttgatca aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa
     4381 tgccttcgtt acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg
     4441 gaagtgttct atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct
     4501 cagcaattct gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc
     4561 ttcgaaacaa tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag
     4621 tctcttcaat tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt
     4681 aggatcaaat attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc
     4741 atcgatcaat aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc
     4801 tcttagaatc gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag
     4861 cggatcatcg gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata
     4921 tgttctcttt tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga
     4981 acaagagtca taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg
     5041 tttcttttta tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt
     5101 agctaaatac agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa
     5161 gcaaagcacg gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt
     5221 taccagcaac aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca
     5281 gatcgtagcc atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat
     5341 ccatcacttc attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca
     5401 ttgggaaact ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg
     5461 ttcctgcttt caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa
     5521 gttcttttgc agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat
     5581 ctgaaatgat agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt
     5641 ctttatatag ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca
     5701 atggattagc tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta
     5761 cttcgctgat atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg
     5821 cattttctgc tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg
     5881 cgtattgtgt ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt
     5941 cattttgcaa gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt
     6001 gaccaaaaac taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata
     6061 atagcaaaaa aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta
     6121 aatattttgt atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg
     6181 taggtggttc gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc
     6241 ccatcgtatg ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt
     6301 tgtctagtcg gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa
     6361 tccaaaccgg tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt
     6421 cccaatgcgt tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta
     6481 aatctgtata cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag
     6541 gttttccgca tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt
     6601 cgatcaaaaa atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat
     6661 ctccccagta aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt
     6721 tccctccgcc aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc
     6781 cttgcataaa cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag
     6841 aatgaacata gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat
     6901 tttaagtaca agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg
     6961 cttcgttatg tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt
     7021 tctttcaaat aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc
     7081 gacgctaagt cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca
     7141 tcaagacatc taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata
     7201 cccggatcgt caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat
     7261 tactgaatac attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat
     7321 tacgcaagtt ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt
     7381 gggtatttga aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc
     7441 tagctaataa cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt
     7501 ttccagcttc acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag
     7561 taatcgtcaa taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt
     7621 gtcccatgaa gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc
     7681 cgtatttagg gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga
     7741 ttgcttttac ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag
     7801 caatacctgt agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt
     7861 cataagaata tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg
     7921 ctaaccaatc catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg
     7981 atgtgattgg acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat
     8041 tgatggcata taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt
     8101 ttccgacaac tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt
     8161 cacgaagctg attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag
     8221 catatgttct gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg
     8281 gtgaaggtcc catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg
     8341 aacgaccatc taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga
     8401 ataattggtt atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt
     8461 ggtcgatcaa ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga
     8521 tatagatatc aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt
     8581 tgattgctgc taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg
     8641 gtttagaaat atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat
     8701 attgttcagt gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag
     8761 tcatatcatg atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac
     8821 gacgatagtc accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt
     8881 tgtttttacg agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg
     8941 ttttgcgcca gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt
     9001 ttagcgcatt gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt
     9061 tgtctgtctg tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg
     9121 aagtaatagt tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga
     9181 tatcatttaa cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca
     9241 aaaagctgtc atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta
     9301 cgccttcttt ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct
     9361 aaatttctgt tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg
     9421 caagggtttt cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc
     9481 aacgttgaca taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat
     9541 aaggatttat taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct
     9601 cttcattaaa atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt
     9661 cgatgacttc tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac
     9721 ctatagggaa atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta
     9781 tttcttttga tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac
     9841 gcatgaccgt cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc
     9901 gtttaacagc tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa
     9961 gcagcccgtt gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct
    10021 ttgctccaac aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta
    10081 ctaagaaaac atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt
    10141 cttccgcctt tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga
    10201 actctgtcat tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct
    10261 ctgcttgaat tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg
    10321 agattgtttg agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga
    10381 cagggcggta gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca
    10441 attcacctga gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag
    10501 cactataaga acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta
    10561 cgtcttcttg tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga
    10621 ttgtcgttag acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa
    10681 cagagaaaag ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca
    10741 agctttcgga ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca
    10801 atgtgacaat tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt
    10861 tggcgtcttt tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata
    10921 aagctttcat caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt
    10981 gactacgttt tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca
    11041 gtccaacttg atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct
    11101 gtagctcagt gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca
    11161 attcgacgac aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat
    11221 cgattttctt attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt
    11281 catatgggat ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg
    11341 ttttcgaacg aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt
    11401 ttccttgcaa gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt
    11461 caaggctcgc ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt
    11521 tatgagtcgg gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa
    11581 ggtttgggta tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca
    11641 cgagatcgac tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc
    11701 tggcttcagt ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt
    11761 gcatttcgat cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat
    11821 aaatactgat gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg
    11881 atttacggaa tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc
    11941 gttgcactgg cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt
    12001 tggaataacg tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt
    12061 gtcgattttc catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt
    12121 atctgctcgt tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc
    12181 ttttttatct aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca
    12241 ttttcttcga ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc
    12301 agcatcatcg atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca
    12361 caattgttcc gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc
    12421 gactttttta atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt
    12481 ctttccttgt cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc
    12541 ttcaatcagc ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc
    12601 gccgtctgta tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga
    12661 aaactcagga ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt
    12721 caagatatct tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat
    12781 tgcttggaac tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac
    12841 taaatacaat tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag
    12901 aagagattcg ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc
    12961 tgcttctctc gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct
    13021 attttcctgc aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc
    13081 aactggcgta ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac
    13141 acggatagaa agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc
    13201 ccgttctttc agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc
    13261 tttcatacca gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa
    13321 aacattttct gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt
    13381 ttctcctgaa aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac
    13441 aaattctttg attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag
    13501 atcagaaaga gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc
    13561 taaaatttca tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac
    13621 agtcgtcccg tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc
    13681 gccgttttta aatttctgct gatactctac gccatctcta acgatcgtta caattagcca
    13741 tttcgataag gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta
    13801 ccctccttga ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg
    13861 tattccggaa gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact
    13921 attgtcttca tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac
    13981 tgcattatcg acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat
    14041 gtacatccct ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc
    14101 attgtattcg ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg
    14161 caaaaccgca ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag
    14221 aaatcaattt taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct
    14281 tctttgtggt catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca
    14341 acgatccatt acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt
    14401 ttcaatccct aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat
    14461 ttcaggtaaa aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc
    14521 tgtcaaattc gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac
    14581 tggtatgatt ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc
    14641 tggtttattg ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat
    14701 cgtttcttgt gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc
    14761 catctattat acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa
    14821 ataaaggata gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt
    14881 ttagcatatc ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa
    14941 aaagatatcc gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta
    15001 ggcaaaacag ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact
    15061 tgtttgccga tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc
    15121 attcttggcc atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca
    15181 agtgcaggaa tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt
    15241 atcgttagtt tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg
    15301 atcactttat ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat
    15361 tggttactga cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac
    15421 aatatcaagc gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc
    15481 aagaaaaaat cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga
    15541 tgtcacactc ccttattact tacaggatag cttttttaca caccttggta aacggtgttc
    15601 cctgctaact tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag
    15661 atttcatggg cacctagttg attgatccct actttttcca tcaagtttcc actactgttc
    15721 accgtgtcag cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa
    15781 ggagaccaga tccctacata aggcatatct ttatagctta aagttacgct atgggaagat
    15841 ttttcacttc gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc
    15901 tcaaaaagtt cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc
    15961 atatcgatga atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc
    16021 agatagaaat cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca
    16081 ccgatagaaa agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg
    16141 ccgtctcctc ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat
    16201 gtctccggcg tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca
    16261 aaatccatat cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca
    16321 tactgatcat cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa
    16381 tacgctggat caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc
    16441 aattccgcac cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc
    16501 attgccattc ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc
    16561 tatctgcccc agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg
    16621 gaattcaata cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca
    16681 gctgattcca attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg
    16741 gcaatattag aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt
    16801 acagccttca attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc
    16861 cgtacatctg cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta
    16921 gctccatatt ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag
    16981 ataatcgtac ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat
    17041 ggatatttct ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta
    17101 gcttcagttt tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca
    17161 acaaacattg ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat
    17221 actccgtcac ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc
    17281 attttatcgt aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg
    17341 tttatcttgc gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa
    17401 tatagcgact taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt
    17461 ctgtgatcgt tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta
    17521 gtttttccaa aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat
    17581 cgaaagcgat ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt
    17641 ctgtaccgat caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct
    17701 tcacaaaatc ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa
    17761 gttctggaat tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga
    17821 cgtgatctgt ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca
    17881 atatgtctct ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga
    17941 tcttatcgat ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac
    18001 tatggatatc agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag
    18061 cttctttgac tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt
    18121 catttaggtc gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt
    18181 ggtcttcaac ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca
    18241 agactgtacg gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat
    18301 tttgctgact agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct
    18361 gttctttttt gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag
    18421 cacttgtgta tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta
    18481 aatcccgaac cattgattct acatctcttc cgacataacc gacttcagta aattttgttg
    18541 cttcgacttt gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt
    18601 taccgacccc tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt
    18661 tttcatctaa ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg
    18721 ctgcgtgctg tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt
    18781 ttaattcata catatctact cacctcttac agttcttcta cgataatatt atgattagta
    18841 aagatacaga tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg
    18901 tcgttttgag catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat
    18961 ccgatagcta agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc
    19021 atttctttat cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc
    19081 cgccattctt gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt
    19141 ttgccttcaa atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca
    19201 acaacttctc cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat
    19261 tcacccattg ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca
    19321 cagatagtgg ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct
    19381 tttccttatt tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt
    19441 gttacatggg catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact
    19501 gtccgcaaat ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga
    19561 ggatggatat cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt
    19621 ccagtaggcg tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga
    19681 tattttgtca tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca
    19741 aaagaaccta gaggagcata tctctctttg ttccctttac catggatcaa catgacctct
    19801 gaatcaaaat caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac
    19861 ccgtaaagga cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg
    19921 ctgtcaaaaa gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc
    19981 tttttgagat gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac
    20041 tgataaaacg aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga
    20101 cgatgatcag ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga
    20161 tcagcgtctc cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct
    20221 tctttcgttt tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc
    20281 cacgtggctt ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg
    20341 tttataaaaa gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat
    20401 tggcgcaatt cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg
    20461 agactatccg tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt
    20521 ctaattctgt ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc
    20581 ttgctttttt gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca
    20641 tcggttggaa atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg
    20701 tttcttgtgg aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag
    20761 caagtagtcc gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa
    20821 agaacagatc ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca
    20881 taaagctgtt tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa
    20941 tcatccggaa aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt
    21001 tgtataaaga agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc
    21061 cagttttagg atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc
    21121 ctcgctgtgc cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag
    21181 atttaagttc tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt
    21241 ctgtcatcgg acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata
    21301 ctttatccat atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa
    21361 aaccatctga accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc
    21421 ctgttgctac tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta
    21481 ctaatggatg ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag
    21541 ctaaggcacc accagctggc actgctgttt catctgccga agtgattact accgaattca
    21601 atcggcgcat ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg
    21661 aattggaaca tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg
    21721 gacgcatttc atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac
    21781 ttccggcaag ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat
    21841 tatttttgga tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg
    21901 actttcttct cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc
    21961 caagaagtga attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta
    22021 gattttcgtt caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt
    22081 acgattggtt tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat
    22141 ttccctaatt ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca
    22201 tctttgattt gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt
    22261 ttatagaaac gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa
    22321 tctccttcca tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt
    22381 aatgaattga caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca
    22441 tagtagcgtc tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg
    22501 ttttcttcta gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa
    22561 tgttgttttg gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga
    22621 atattttctt tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct
    22681 ttgaatttga tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact
    22741 ttcattgtat ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat
    22801 gtataaagtt tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc
    22861 acactcgttg gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt
    22921 cggtgatcat gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct
    22981 gcttttgctg tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact
    23041 gttccttgtt tacccaaagt gattccttca tataattgct gtgctaccat catggttttt
    23101 cttgttcgaa agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga
    23161 ttcgcagggt ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca
    23221 tcaattctag cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg
    23281 tcgattcccc agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg
    23341 ctccaatatt cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt
    23401 aaagcgacag attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata
    23461 ggactgatcg aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact
    23521 aaatctacat cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt
    23581 tcattgaaga ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc
    23641 caagcaatcg cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt
    23701 tttgctgcgg ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg
    23761 ggttcataat tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg
    23821 ccaacactgg ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt
    23881 gcaggtgatt ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat
    23941 tgtcgtgttc ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca
    24001 gaaaacgttt gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa
    24061 gatatcttca cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt
    24121 agaatgttct gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc
    24181 ttgctgtgct gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt
    24241 agacaaagct gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg
    24301 aggaggatat tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt
    24361 ttcagcagga tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg
    24421 agagattgct gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac
    24481 atatccagct tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga
    24541 agtgtgtctt ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt
    24601 ataaaacagt acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc
    24661 aaagcaagtg ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg
    24721 ccaacttttg cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc
    24781 aaatccgatt tctttataag gatactgcat catccagtta gcaagtgccc attttccttg
    24841 actactgatt ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat
    24901 aaaaccgcct ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta
    24961 aatatttttt tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa
    25021 gtccttctaa gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat
    25081 aaagcacgtc atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag
    25141 cagcaatcga cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat
    25201 ccaaggtcat cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt
    25261 ggatcatcgc taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa
    25321 cgatcccgat cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct
    25381 ttttctctga taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga
    25441 taactgcagc tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg
    25501 caatccattg gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt
    25561 tttccttaat taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca
    25621 cgcctttgcg tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc
    25681 ttttcaatcg ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct
    25741 tcgcaacgat ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat
    25801 tcgctgccac gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat
    25861 tccggtgcca atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct
    25921 gaataatatc gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc
    25981 tggtctttga tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg
    26041 ggccataaaa tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc
    26101 tgaccttttg ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga
    26161 ttcatcaacg tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga
    26221 ggcttgatcc cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca
    26281 ggtactagac ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct
    26341 tgtttacgaa aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta
    26401 ttcaataaaa caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa
    26461 gataaaggta atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca
    26521 gaaacttctc gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc
    26581 tccattcctt tcataccgga atattccccg gctttacttg tttttttctt tttcaatata
    26641 aattcacttt tttattataa aga
//
LOCUS       DAZAID010000032        26515 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000032 DAZAID010000000
VERSION     DAZAID010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.032"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..361
                     /locus_tag="ACG9BE_001243"
     CDS             <1..361
                     /locus_tag="ACG9BE_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032000.1"
                     /translation="AGSTMDHLYIKYIHQFSVLVLGTLIIAGIVAFIIQGRDILTVHQ
                     EFFSYAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLPPKIFTLLGIQCLSFSFISYII
                     VLIFLSFIVWMFYLVKK"
     gene            456..1340
                     /locus_tag="ACG9BE_001244"
     CDS             456..1340
                     /locus_tag="ACG9BE_001244"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN3032001.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1564..4731
                     /locus_tag="ACG9BE_001245"
     CDS             1564..4731
                     /locus_tag="ACG9BE_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFN3032002.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4799..5296
                     /locus_tag="ACG9BE_001246"
     CDS             4799..5296
                     /locus_tag="ACG9BE_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFN3032003.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5315..5779
                     /locus_tag="ACG9BE_001247"
                     /pseudo
     CDS             5315..5779
                     /locus_tag="ACG9BE_001247"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5776..6534
                     /locus_tag="ACG9BE_001248"
     CDS             5776..6534
                     /locus_tag="ACG9BE_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN3032004.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6620..7360)
                     /locus_tag="ACG9BE_001249"
     CDS             complement(6620..7360)
                     /locus_tag="ACG9BE_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN3032005.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7421..8674
                     /locus_tag="ACG9BE_001250"
     CDS             7421..8674
                     /locus_tag="ACG9BE_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN3032006.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            8996..9223
                     /locus_tag="ACG9BE_001251"
     CDS             8996..9223
                     /locus_tag="ACG9BE_001251"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFN3032007.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9346..9672
                     /locus_tag="ACG9BE_001252"
     CDS             9346..9672
                     /locus_tag="ACG9BE_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFN3032008.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9740..10453
                     /locus_tag="ACG9BE_001253"
     CDS             9740..10453
                     /locus_tag="ACG9BE_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFN3032009.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10763..11613
                     /gene="licT"
                     /locus_tag="ACG9BE_001254"
                     /pseudo
     CDS             10763..11613
                     /gene="licT"
                     /locus_tag="ACG9BE_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            11734..13599
                     /locus_tag="ACG9BE_001255"
     CDS             11734..13599
                     /locus_tag="ACG9BE_001255"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN3032010.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13613..15056
                     /locus_tag="ACG9BE_001256"
                     /pseudo
     CDS             13613..15056
                     /locus_tag="ACG9BE_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            15164..15865
                     /locus_tag="ACG9BE_001257"
     CDS             15164..15865
                     /locus_tag="ACG9BE_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3032011.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            15991..16503
                     /locus_tag="ACG9BE_001258"
                     /pseudo
     CDS             15991..16503
                     /locus_tag="ACG9BE_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(16901..17110)
                     /locus_tag="ACG9BE_001259"
     CDS             complement(16901..17110)
                     /locus_tag="ACG9BE_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFN3032012.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            17351..17926
                     /locus_tag="ACG9BE_001260"
     CDS             17351..17926
                     /locus_tag="ACG9BE_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFN3032013.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            17926..18366
                     /locus_tag="ACG9BE_001261"
     CDS             17926..18366
                     /locus_tag="ACG9BE_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFN3032014.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            18382..19083
                     /locus_tag="ACG9BE_001262"
     CDS             18382..19083
                     /locus_tag="ACG9BE_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN3032015.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(19571..19996)
                     /locus_tag="ACG9BE_001263"
     CDS             complement(19571..19996)
                     /locus_tag="ACG9BE_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032016.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            20370..21341
                     /locus_tag="ACG9BE_001264"
     CDS             20370..21341
                     /locus_tag="ACG9BE_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3032017.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            21373..22770
                     /locus_tag="ACG9BE_001265"
     CDS             21373..22770
                     /locus_tag="ACG9BE_001265"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3032018.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            22929..23552
                     /locus_tag="ACG9BE_001266"
     CDS             22929..23552
                     /locus_tag="ACG9BE_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032019.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            24301..26394
                     /locus_tag="ACG9BE_001267"
     CDS             24301..26394
                     /locus_tag="ACG9BE_001267"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN3032020.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         8914 a         4424 c         5606 g         7571 t
ORIGIN      
        1 ggcaggaagt acaatggatc atttgtatat aaaatatatt catcaatttt ccgttttggt
       61 gttaggcaca ctaattatag cggggattgt cgcatttatt attcaaggcc gtgacatatt
      121 aacagtacat caagagtttt tcagttatgc atttggtatt cctctgatcg ctataaggtc
      181 tgataacaaa aagtggaaga aacgctatta cattctcttg ctatttttgt gtttttatct
      241 gccacctaag atttttactt tattaggtat acaatgccta tcattttctt ttatttccta
      301 tatcattgtt ttaattttcc tcagtttcat agtatggatg ttttatcttg tcaaaaaata
      361 aatgaatgtt tcatgaacag tacttcatca cagcctaatt tttttcttta tgctatcgtt
      421 atattaaaga aatcaatcag aagggaaaga aagtgatgaa aaaagtttta ttagtggtaa
      481 accccagttc aggcggtgaa caagcaaagg aatttgagca attagcgata gcaaaattgg
      541 aatcggtgtt tgatgaagtg gtcgtcttgc atacaaaaaa agcaggggat gcaaaaaact
      601 ttactcgcga agcggctacg gaagggtatc atagtgtgtt tgtcatgggc ggagatggaa
      661 cagtcaacga aggaatcagt ggaatcgctg aacaagaaca tcggccaaat ttcggatttt
      721 tcccattggg tacggtgaat gacttggctc gagcattagg gataccgcta gaacctgagg
      781 aagcgatcaa tcatttttcg attgaatcag tcaagccatt ggatattgga aaaataaatg
      841 acgactattt tatgaatgtc gtcgcaattg gctctatccc tgaatcaatc aatgatgtag
      901 atgcggagaa gaagacgaaa tttggaaaac tagcttattt catgtcagga atcaaacagt
      961 tagcttccac acaatcctac tcgtttcacg tagaagttga tggaaagaag gaagaaattg
     1021 agagcagtac cttgctgata ggactaacta actcagtcgg cggttttgaa acattacttc
     1081 caaatgcaaa agtggatgat ggtaagctcc atttggttta tttaaaagat tcttctttga
     1141 tcgatacgct taaaacattg ccagacctat taaaaggagt agatgaatct acgaaaaatc
     1201 tagcctacct tacttgtgag caattagttg tgtcattagc tgatgaggaa gaattgacga
     1261 cgaatgttga tggagatgaa ggggataaat tacctgtgac aatcaaagta ttgccctcac
     1321 atttacaggt ttattgttaa aatggaggtt gtgaccgagg agttggcggg agaacaccgc
     1381 ttgttcggga taagggactg tgacaagact tttgtcacag cctttttttt gtacaaaaaa
     1441 gaaatttaca cctaatagaa aaatttttcc ataaaaatac atattttaaa tatgaaagta
     1501 caattggaaa tagattaaaa cgataaaata aaattgtcaa aacgtcagag aggatggtgg
     1561 agaatgaatc aatgtgtaaa aaagctgatt gcgtcgattt tgtttgtctt ccctctcttg
     1621 atggtgacat ccgtttttgc cgatgtagag aaaacaaacg aatcaacgca atcagagaat
     1681 caaagttttg acaagactac tttttcagaa gaatcgatta cttctgaaac tacagaatct
     1741 caaagtgagg cttctactaa taaaaggcaa gaagaaaata agacaagtga gacctcagaa
     1801 gtagctgaac aaacgtctga tagcagtgaa gcggcactgg caagagctgt ttcagcggat
     1861 gggtacagcg aagctgccac aacagaagaa ctggcgcagc ttctcgcaga cgaatcagta
     1921 actaagatcc gactgattca gcctttgact ttagacaggg aattagagat caaacgagat
     1981 atcgttattg attttggtgg attcgctcac aatttcggca cacaccatat ttatatcaat
     2041 gagatacctc agtcgatcga gttccagaat tttaaaggaa cagctgcaca tccaggtgga
     2101 ttgacaccaa atatcgatgg aaatgccatt atcattgctt atatgaccga tttttgggga
     2161 agcagatatc attttacggg agaaataaaa tttaccggga cacttgattt gtatgatggg
     2221 tccaaattag gactgattta cgctcctcgt gcaactgtca cattagatgg tgtttccggt
     2281 gtgttggatg tccaaccagt gaaggaagga ctgaatgccg ctccgggtaa agcctatttt
     2341 tgtagaagct atcaattgaa tgtgatcaat ggtagtcaac tttatggacc gtatctaggg
     2401 aaattctacg gttttctaga ttcaggagat gagactggta gtggaaaaac agcgccagga
     2461 atccatatct taagcggttc aaaggtctct cttgattatg atcgggcaga tggcgttagc
     2521 gatgaaggag aggcagtcga tacgctacct ggtaatgtca tgtttgaagt cagtggttcg
     2581 ggaagcgaat tttcggtaaa tacaaagatc aatattacca atgacaacaa tcgcggaatc
     2641 atccaaatga gaggttcagg atcgaaagtc actgtatcga atggtgggaa aatcaccatc
     2701 aatacagcaa ctacgggcgg atttcgcttg caaggagatt ctagtcaaat ccatgtatca
     2761 agtggtggac aaatctcggt caaaatggct ggagatgacg atcaacccgg gaacaatggg
     2821 atgcgttttg taggaaaagg cctttctttg atcgtagaag gagccggatc agttatcgac
     2881 atcgataagc agagtgggcg ttccagcgca gttcgttttg agaatggtac gcaaaagctg
     2941 acagtaacca atggaggctc attgagggta agaaatacag gtgatggcaa atcttacgtg
     3001 aacagactca atcaaggaaa tcaagcgatc caattttatg acgcttctgg ttttctagaa
     3061 agtccaggtt cagctgattt tacagtcact ggagaaaaat caaatatcga tatacgcgca
     3121 gattccggag caactgtaga tacaactgga gatattgatt taaattttac ggcaggtgaa
     3181 aaaacttatt tagtcatggt cgggaaaaca ggggaagata agaccggcat catcagtggg
     3241 gatgtgttga atgtggactt agaatctcct acctattttg atttccaaaa caaacggatt
     3301 ggaagcgaga ccaatggcat aggcggctgg gtattccaag gaaatggaga ttcttcttta
     3361 aagatcgccc attcagaaat cgctttatgg cgaaaaacag gcgcaacatt cgataacttt
     3421 gaaaaagacc cggattattt ttctgatatg acagacttat cgatcaatgg gaccgactta
     3481 ggcaatttcg tatcaagccc tagtgcaggt ttaacaaatg aatttgctca gacaggtgcc
     3541 ggaatgaaaa attataatcg aatcagtgcc aataatcagc tcccgatgat tgaatcctta
     3601 agagtgccaa cgaatgcaga taaaaaaatc tatgggcatg tcgttgttcc cgaaggtgtc
     3661 gaggcaaagc caagagatgc ttggacaggc gaagtagaag tgactttacg tattacttac
     3721 gcagatacga cgaaatcatc tgtagaaatt actgctctca cgcaaggaca aacagatgaa
     3781 aatgaagggg caggatataa cccatgggga gaaggcgaac aaggcggctt gttcgaagtt
     3841 caagtaccca atgatgagag attaaaaacg gaggatcaag tgcaagttgt tgctgctaaa
     3901 aaagtaagag gtggccaaga agtgacagaa ctggactctg ccaaaacgac agtcgatgtg
     3961 attcctcctg aacctgctaa tttaagtacc acattgatca atgcagcttc tcgcacgatc
     4021 acaggtacag gtgaagcagg agccttggtt tcattgaaaa aaaatcaagc tgttttgtca
     4081 gaaacaacta ttgatgatac aggggaattt gttctttcta tcccagaaaa tcaacttttt
     4141 gcaggagatg tattagaaat tgtattaaac gaccaagcag gagaagctac tgctaagggt
     4201 gtgcagaaca agccaagtac caacgatgaa attgggaatc acaatccaag tgatacgcca
     4261 gttgtttatc atgatgcccc gccttttgca cctgctacga agatcgtagt agaaggtggt
     4321 ctctcatttc ttgccccttc caaactcgat tttggtcaga tcaaagcaac tggtttgaca
     4381 caagaagcat ttggccaaat ggatatacag gaaaaattat ggatttatga tcagcgagaa
     4441 acgaaaaatc cgtggatctt aacgataaaa ttatctcagc ctttcacaaa tgatgcagga
     4501 ttttctatgc aggatgtcct atatttcaag aaaggagagg agtatcaatt agtgaatgaa
     4561 gaagaactgg aaattgtcag aggacaaaat tcaacgaatg tgcaagcaga ttattcagaa
     4621 tatttaaata acggtcgggc gtttaaactt gtcttaacca aagaaaatca gatagctggt
     4681 aattactcag cagagataac atggacatta gcagatgcgc cgacagaata aagatcaagg
     4741 aggatgattg atgaatcgaa tatggaataa aagtcatcga aacaactgct ctttacttat
     4801 gatccttctc cttggattca tcctttggca gacacctgtc catgcctccg aattcaattt
     4861 tgcggtagat tttgaattac cggctaatca gattgataag aacaaaacat atataaatgt
     4921 aaggatggag cctgggaaaa cacaggaaat cccctacacg gtctttaacc gaacagataa
     4981 atcagtaaca gttgagacat tgatccactc cgctacgacg aattcaaacg gtgtgatcga
     5041 atatggagaa tcaaaaagta aatttgatga aacattaagt tatgatatga aaaacttagc
     5101 aaaaacagtg ggagaaataa ctgttcctcc tcaaagtgcg gtcactgaga cactttcact
     5161 gaaagcgcca gaaaaagagc tggctggtgt ggtcgcagga gggatcacgt ttcaagaaaa
     5221 aaaagagaac gtttctaaca aaagtacagt tggtgtcgtc aatcagtttt ctcaagtgaa
     5281 agcaatcttg atttgaaata gcgaaaaaga gtcttcccca gatttgaaat taaaaaaagc
     5341 ttatgcggat caaagcgatg gaagaaatgt gattaaggcg aatcttcaaa atccaaatgc
     5401 ttcttatatg ttcggcttga ctacatcggt tgaaatctac aaaaaagaaa caaaagagcc
     5461 ttacctttca ataaaaaaag aaaaaataga tgtagcaccc aatacaaatt ttgatttact
     5521 tgtcccatta gaaggaaaaa aattagctcc gggacattat caagcggttg tgaagggtgc
     5581 ttggaaagaa aaacgctgga atctccatac cgattttgaa ataactgatg atgcagcgga
     5641 ccaattgaat gaaaaggata ttgatttaaa aaatcagcca gaaacatttg actggcgatg
     5701 gctgatactt gttggagtca ttcttattgg cttatttgct ggattattta ttttaaaaga
     5761 aagaaggaaa aagaaatgaa aaaaacaatt gcattaagta ccgttctagc tggaagtcta
     5821 ttattatgta cacaaagtgt ttttgctgag aataagagct cagccactac agaaggaacg
     5881 atttctgtga ccaaaccaga tgctgcaaat cctgaagacg ttgttccagt tcctcctgtt
     5941 gatccaaatg atcctgatac tccttttaca cctgacgaac caaatcaggg aacatccgga
     6001 ttactgacga ttgaccaagc tccagatctt gattttggaa caatcaagtt aggtacagca
     6061 aaaacagtat atgctgcatt acaaagcgga acggacagta ctggagcaca aacacaagta
     6121 cagaatttcg tacaagtaac ggataaaagc ggaaattatg ccggttggaa attatcagtc
     6181 aaacgaacag aatttattgg tacaacatct gatacgaata aactaactgg ttcaaaggtg
     6241 aaattcaaaa acgccgtgat tcgaaatagc gcaactaata gcagtggaat gccatctacc
     6301 gtttctggaa ctggcgctga aggaatcgaa atcccagcag atcaggcagt agatctagta
     6361 actgcaggag caaatgaagg gattggcaca tggatttatt cattaggaga gaatgtggaa
     6421 caagggagac aatcagtgga attatttgtt cctgtatcca attatgctgt agacaactac
     6481 gtttcaacat tcacatggtc gttaacagat gctcctacag aagaggaagc gtaaaatcat
     6541 atcaacgatt tcagggagtg gagccgattc aaggcccact ccctgaaatc tgctatgctt
     6601 acgtctcagc tcttattcat caactcgaag cagttttttg gaagtcatgg aaacgttcct
     6661 ttgtcttttc aaagatcttt cgaccaacga gtaaggagtc accgttgaaa aattctaaca
     6721 cataatgagc agaatcaatt ttctgcaatt gcgataagtt gatacagtaa gattttaaaa
     6781 gtaaaaagta agcatacttt tctaactctg tcttcaattg ttttaaagaa gtattcacta
     6841 atttatcttc tgtagttgtt ttgaccataa cttggttttt caaacctttg atactttcaa
     6901 tatataaaat ttcccgagga tacaggacga gacttccacc tttttgatca ataaagattt
     6961 tatcgacatt tacgatctgc tgttctttcc aattgatctg ttctagcact tgacgaattt
     7021 gttcagataa attcctgtct tttctgatat atccaatcgg aaggattttc cgattgatga
     7081 tctctactgc aaaattcgta taagcggtga aaaaaacaat attactatgt gcattttttt
     7141 tacgtatttg ttcggcgaca tcaatacctg tgagtgattg cttcaagtcg atatcgataa
     7201 agaaaacatc gtgattatca atagtgccca actggatcaa ctgattgatt tgagagatag
     7261 aagtgagctg cacgatattc tcgatatgat gattgaccaa ttctctgatg atccattcgc
     7321 tgtgaaactg gttatcttct atgataaaat actttttcat atgcaggctc ctattaaaaa
     7381 aatatgtgta cattttgaaa caaaacaatg gaggaataag atggtcgcaa cagtgacact
     7441 ttatttgacc tttactttga ttttagattt aggtatctat ttattctttt cgaaacaact
     7501 gctagtaaaa aacagctttg gttgtatagc tttgatggtg gtgacacttt tacttcctat
     7561 ccctaaagtg agctttcctt acctagttgg cttattattg atgtttcttt tttattttac
     7621 tacgaaaaac ctgtttatca gctgtttgat cagtaatctt ttttttgcta tcactggact
     7681 cagctttttt ctggtttttg atctgatgga ttatttagga aagagacatc ttggaacagc
     7741 aagttttttt gcactactta tcgcatttat tttctatcta ttagtaaaaa gtatagacaa
     7801 acagacgaga tttctcaact tgctttttac ttatccaact gaacactatt ttggaggtat
     7861 ttgtatttta ttaagctggg gcgtgattta cttggtattg ggaagtgttc acttccgctc
     7921 atctaattat ctgcttctat ctcttttgtc gatagtcatc agtttttttt ctttgttttt
     7981 ttccattatg ttgatcagtt tccaagtaaa agaaagacaa catcaggaat cgcttcaact
     8041 gtatcaaata aatgaagaat attaccatca tctagaggaa ttcaaacatg attataaaag
     8101 tctactgttt tcattaaaaa ttactcttca ggaaaataaa aaagaaacgt tggaattttt
     8161 agaaatatta gaagatcatt ctgcagctat ttttgaaaaa ccgcaaatac aacagctaac
     8221 aaatattgta tccccagctg tcaggggtgt ctttttaaaa tttctagaaa aagcagaatc
     8281 ggagaaaatc ccgatccaag tagttattcc tgtcgaagtg aaaacaatcc cgattgatct
     8341 ggttgttttt attcgctgtt tatctatttt tatttctaac gcattcgatc atcgtgtagc
     8401 cgatcagagt ccgattacga tcaatctgct ggaagaaaaa gagggatcac gttttagtat
     8461 agcgaacaag gcaaatccaa cgaatttgac gctatcggag atggtcaaaa gaggaaaaac
     8521 ctctaaaaaa aatggcgggt taggtctata cagtgcgaaa aaaatgttgg atgtttacga
     8581 aaatgcagaa ctaaaaatcg aattttcaaa acagaatcac tatttttttg tagaaatgta
     8641 tctggcatct agaaagagca aatcagccta ctgacttact gtcaaaggat aagtttgagg
     8701 aaagtctaca cggagataga gacaacaaca cagcatattc aaaagaaaca gaagcgtgat
     8761 gaccgttgtc acccttctgt ttcttttgct atgtagttaa acaaagtagt tgacacttcc
     8821 gctacttagt attagtacga aagtatactt catgatacta agtagataag aagtgaaaag
     8881 cggaacccac gatcgtcaaa atgctaactg ctttgcttac accagataaa gggaagagca
     8941 atatcgctta ttttaggcgt ggagaagcaa gcaaatccct acgatcacaa aaaaaatgct
     9001 gacccagcag ttaagagagt tagaacaaga tgaaagtatc catcgaaaag cctataacca
     9061 agtcccgcct aaagtagagt actcgctcac cgaagaagga aaatcattaa gagagattct
     9121 agtagcgttg tctgtttggg aagaagaacg tatcgaaaaa caaaaagcct caagggaaga
     9181 agacacgcta ctgcatacac atggtggcta cctaaattat tgattttcta tgacatttat
     9241 tttaaataaa agaaaacagg agttttttta aaagcatcat ctatttttta tgtaaaaaaa
     9301 tggtatgatt aatcaagtaa gaagtgattg gaagaaggca agcaaatgaa tagtatggaa
     9361 aaagtcaacc agtttagaga tgaacgtaat tggcgacaat ttcataacga aaaagatttg
     9421 gctatctcta tttctttgga agcatctgaa ttgttagaac tttttcaatg gaaaacgccg
     9481 gaagaagtaa aagaaacaca attagaacga atcaaagaag aactggcaga tgtattgatc
     9541 tattcataca tgatggcaga caaccttcac ctggatctag atgagatcat tgaagaaaaa
     9601 ttgatcaaaa ataatttgaa atatcctgta gaaaaaagcg caggtgtacg aaaaaaatac
     9661 acagagttgt agaaatgtgt cattcggctg ttggaaagag caaaagaaaa acagtaaaat
     9721 gagtctggag ggagagaaaa tgaaaaaaaa aggaaaagca aaaagatggc tagtcaatat
     9781 cctcttgttc ctcttgttat tagtaggact agctttgatt ttcaatgaac agatcaagga
     9841 ttattttgtc agagagacag gagataaata tgctatagcg aatgtaacaa aagaagatct
     9901 gaaaaagaat aacgataaag atgtcagttt tgactttgat gcggtagaac caatgacgac
     9961 agaaggggtc atgaggtcgc aaatgagagg aacagatcta ccagtgattg ctagtatcgc
    10021 agtcccttca gtttcaatca atttaccggt tttcaaaggc ttggataata cgtcactact
    10081 ttacggagca ggaacattat ctccagatca agaaatggga aaaggaaatt atgccttagc
    10141 aagccatcgt gcaacgaacc ctgaattgct gtttacaccg ctagaaaatt tagagatggg
    10201 tgccaagatc tatttgacag atttagaaaa tgtgtataca tacaaaacat tttttaaaga
    10261 aaaagttgct cctacagata cccaattact gaatgaagtc gagggaaaag aaattgtcac
    10321 actaattact tgtggcgaca tggacgccgt cacgcgacta gttgtacaag gagaactgga
    10381 aagtgttaca tcgataaaag atgcgacaga tgatatgaga tctgctttca atctagagac
    10441 aaaaacattt taggatactg ggacataagt cgttacagat taaaaacagc caaaggatat
    10501 gaatcgataa ggaaaaacca atttgctgaa aaaaagagaa acgcaaagag gtgcatctct
    10561 gatttggttg ttttctagat agacttgtgt tccagttttt ttatttttta tatgaaaagg
    10621 cttgcataaa taaaaattcg atggtatgat gattttgaat aagaaatgga ttgttactgt
    10681 acggcaggca aaaccagaat tcagctaaga aacagtaggc gaattctagg tttttttatt
    10741 tttcttgaga gggcgaagca aaatgaaaat tgataaaatt ttgaataaca atgtcgtcat
    10801 ttcgaaaaat ggattcgggg aagaagtcgt ttgtatggga aaaggactag cttttcaaaa
    10861 aaagattggg gacgagattt cgccagaagc ggtccaaaaa gaatttgttt aagagattct
    10921 tttgctaaaa accaattcca gcagctgatg gctgatattc cggcagagga gatcgaatgg
    10981 gtcaaacaag tagtcgaact ggctgaagat aaactaaaag ttgaattttc gcctaatatt
    11041 tatctgacat tgaccgatca tcttcattat gcgatcacac gagcaaaaga aaatatccaa
    11101 ctgcctaatc cattattatt tgaaacaaaa aaattttatc cgaaagaata tcaagcagcg
    11161 aaagaggctc ttttgttcgt gaaacaaaag acgggtgtgg aactttctga ggatgaagcc
    11221 ggttttattg cctttcattt tgtgaatagc caacaaggaa acgaagacat gcaagtgacg
    11281 atgaccgcta ccacaatggt aagagatata ctgagtatca tcagtaaatt cttcggcatc
    11341 gtgttcgatg aagaatcttt gaattatcag cgtatcatta ctcatctgca gttttttgcc
    11401 cagcgttatc tcaaagggga aggatctgac gagcaagatg aatttctcta cgcattagtt
    11461 caaggaaagt atccaaaagc gtttcattgt gtcgaacgga tcaatgagta tcttcgacaa
    11521 acgcaaaaac aggaaatggg gatcgcagag cagatttatt taacgattca tattcaaaga
    11581 gtagtaagtg agaaaaaaag catccaatca taaggattgt tactgtatgg caggcaaaac
    11641 ctggattgaa aaaataggtt cacgaatgtt tgtgactgtt tttttcaatc tgggttttta
    11701 ttttttaaat aaaaaagaaa gaaggaaaaa acaatggata atcaagcagt tggtcgtcgt
    11761 gtatgggaag cggtaggagg acaaaagaat gtcaaaagtt tagtacattg tgctacgcgg
    11821 ttgcgattca gattaaaaga tgaatcttta gcagatacgc aaaagttaaa agaagacccg
    11881 gacgtgatcc aagtcgtaca aagtggcggt cagtatcaag tagtgattgg cagtaatgta
    11941 gcagatgttt atcaatcgat cgtggatgag gaaggtcttg cagtttcgga ggatggaaaa
    12001 gaagagtcca aaaatatttt gaatcgtctg atcgatatta tctctagtat atttacacct
    12061 tttctagggg cgatggcagc cgccggggta ttgaaaggat tcttatcatt ggcaacggtg
    12121 atgggctggc tgacagctga tagcggtact tatcaaatat tgtttgctgc agcagatggt
    12181 gtctttacct tcttacctgt catgcttgct tttacagcag ctaaaaagtt caaagccaac
    12241 cagtttttag cagtagcaat tgctatggca cttgtgtatc cagctatcac agcagttgct
    12301 ggtgcgggag aagctatcag tttctttggg atcccagtga ttctttcacc atctggttat
    12361 acttcttctg tcataccaat cattctagcc gtatgggttc aaagtaaatt agagccattc
    12421 gtaaaaaaag tgattccgca atttctgcaa atgattttag ttccgcttgt cgttttagta
    12481 gtgatggttc cattaacatt tcttgcttta ggaccaatcg ggacggttgc cggtaatgct
    12541 ttaggcggtc tcttcaactc gatttatgga tttagtccaa tcgtagcagg tctaatcatg
    12601 ggaagtttat ggcaagtatt cgtgatgttc ggcatgcatt ggggattcgt accgatcatg
    12661 tttttgaata tcgaacaata tgggtttgat gtattgatgc caatgcttct tccagccatc
    12721 ttggcacaag gtggggctgc tttagctgta gcattgcgaa caaaagatac gaaacttcga
    12781 gcattaggga tttcttctac agtgacttcg ttatttggaa tcactgaacc aactgtgtat
    12841 ggtgtgacat tacctttgaa gaaaccattc attgccgctt gtatttctgg aggtattggt
    12901 ggagctatta tcggattttc cggagtaaaa gctttctcca gcagtttggt cagcttattg
    12961 acgattccga ctttcattaa tacggtggat ggtgtagagt caaatgtgac agtagcagta
    13021 atagctacag gaattgcttt tgttcttgca ttcgtaggta cattgatttt aggatttgac
    13081 gaacaaacac aagacaatca attagaaaac aaacatgcga atgcaggaga accgattact
    13141 tcagcacggc ataccttaaa aagtccttta acgggtaaag tactgccatt gagtgaagta
    13201 ccagaccaag ttttttcttc tggcgtgatg ggaaaaggta tcgctattga tccagaagta
    13261 ggagaactag ttgcacctgc agacggagag atcacgacta tttttccaac tggacatgct
    13321 gtagggatca caacgactga cggagcggag attttgattc atatcgggat ggatacagta
    13381 gaattaaatg ggaatggctt tgagatctta gtcaaacaag gggatctcgt caaagcagga
    13441 gatttattga ttcgttttga catcgaagca attagagcag ctggctatag cgtaatcact
    13501 ccagttgtca ttacgaatac tgatgcattt gccgatatac ttgaattaga ccaaaaagag
    13561 attattgcaa acgaagatgt tctggcaatt gtaaaataag gaggaaatca ggatgcaatc
    13621 acgtttttca gaaaaatttt tatggggtgg agcaactgct gccaaatcaa ttagaagggg
    13681 cttatgatcg agacggaaaa ggactatccg ttgcagatgc tatgcctgga ggtaagcagc
    13741 gctttgcgat tatcggcagt gaagaatttg actggacgat tgatcaagaa aaatatattt
    13801 acccgaatca tcgaggaatc gatcattacg atcgtttcaa agaagatctt gccttgtttg
    13861 cggaaatggg attcaaatgt tatcgttttt cgattgcctg gacacgaatt tttccaaatg
    13921 gagacgaagg tactcctaac gaagcgggat tagaatttta tgatcagctg attgatgagt
    13981 gcttaaaata tgatattgaa cctgtgatca cgatttctca ttacgaaatg cctcttcatt
    14041 tggcaaaaga atacggtggc tggaaaaatc gaaaactgat cgacttctat gaacgatttg
    14101 ctcagacagt attagagaga tacagcagta aagtcaaata ttggatgact ttcaatgaaa
    14161 tcaattcggc ctttcacttt ccagctttaa gtcaaggatt agtaaaaagc aatggagcag
    14221 gagagtacca aaatattttc caagcatggc ataatcaatt cgttgcaagt agcaaagccg
    14281 taaaaatcgg ccacgaattg cgttcggata tccaaatcgg ctgcatgatt atctatgcaa
    14341 caacttacag catcgatgcc aatccagtca atcaggcagc aacaatgatc caaaaccaag
    14401 aattcaattt tttctgcacg gatgtccaag tacgtggaga ataccctgca tacacagcgc
    14461 gtacccataa aaaatatgga gtcgatccgg aaaaattaga gcaaacggaa aaagacttcc
    14521 agttgctaaa agaatatcca gtggactaca tcggatttag ttattatatg tctacggcaa
    14581 tcaatgagac tgatccacaa gcagcaactt cagaagggaa cttgctggga ggagtaaaaa
    14641 atccgtttct tgaggcaagt gaatggggct ggcagatcga tccagaaggg ctgagaatcg
    14701 cattgaatga attatacaat cgttaccaga aaccattatt cattgttgaa aacggtcttg
    14761 gagccattga tcatatggaa gaggatggca cgattattga tgattatcgg atcgattatt
    14821 tacgtcgtca tatcgaagcg atggctaatg cagtagcaga cggtgtagat ttgatgggct
    14881 atacgccttg gggatgtatc gatcttgtca gtgcatcaac aggagaaatg agtaaacgtt
    14941 acggctttat ctatgtggat ctagacgatg aaggaaatgg gacactgaat cgatcaaaga
    15001 aaaaatcttt tgattggtat aaagaagtaa ttgcctcaaa tggcgtaaac ttgtaattaa
    15061 agcagctgaa tgaaaaagaa cacacttctc acatgtgatt tgtgttcttt ttttattttt
    15121 tatgtgctat tcttttagta atagtaaagc aaagggtgac gttatgaaaa ggacacgtgt
    15181 actatacttg gaagtcgcag ataaaattaa agaagatatt ttctcaggaa aatatccagt
    15241 cggttcgatg ttgccgacag aaagtgaatt agaagaactg tttcaagtca gcaaaatcac
    15301 tgtccgaaag gcaatcgaac tgctggcatc agatgaatat gtcgcaaaga aaagtggtcg
    15361 tgggacgacg gttctaagca atcgtcctta caatcgatta tccaaagcgg caagctttac
    15421 gcaaatcttg gagaactccc gatatgaagt ccggaaagaa acgctggatt tgcaattggt
    15481 ccatataaag aaggaagatc ctttgttttc ctttttcggt gagcaagctg tttgttttaa
    15541 gcgcttatat tatttgaaca atcagcctta tatttatttt gttcattatc ttccacaaga
    15601 aatgatgcaa ttcagcagag aagaatttgg caaagaatct ttgtatcgtt tgctgacgaa
    15661 aaaaggatat actattgact cattttctga tgatttctcg gcagtatttt taacagaaga
    15721 agaacaagga attctgaaaa cagacgcaca gatcggcatc aaacggatac ggaaatcgtc
    15781 tacgcaaatt ggggaaatga ttgaatattc ggaggcggtc taccatacgg agctgcatcc
    15841 ttaccatatt gattatgaaa cataaataaa aaaagaggtt gtgaaaaagc tgccctgcat
    15901 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttaggctta
    15961 ttaccgcaga tacacggctg tttctggatt accatttatt ctggataagg gggtgtggca
    16021 cgattttcag aaggaatcta tgaaccaatc actgtattga ttttagcttc gatggtaacc
    16081 ccattcggct tgacgatcgc ttatttctta gggaaaatca ttcgtaaaaa tatactcaat
    16141 cgtcaagaaa tcgatacatt gaagaccgct tttcctatgg ggatctgtca gatcacagag
    16201 ggatgtttcc ctatcgtatt gaacgacttg gcacgcaatg tcatagcaac gggagtcggt
    16261 gggacagtag gtggcggatt aagcatgttt tggggcgcag atagccgtat tccggcatcg
    16321 ggaatgtttg cagtcgctac gatgaccaga ccatgggcat tcattggtgc attgttagct
    16381 ggttcttttg tcacaggaat aaccatttta ttattgaaaa aaccagtaga tccgaatgca
    16441 gaaatcatcc aagaagaaaa agaggaagaa gacatatctt gggatgattt gacaattagt
    16501 taggagaggc agaatggatt acaagaaaaa agtacaatag atgtaccttg agacaggaat
    16561 ggtatgaaag aatcagagtt agaaagaatc gaatatacag attttggttt aggagcgatc
    16621 gaaactgtcc agagcatcgg caccctcctt gaaatgacga tgaaggaaaa atgagaacct
    16681 ttcgttgtcg aaagggtctg gtatacctta tgtcgaagga aaggaaacaa aaaaactttc
    16741 ctgtactatt ccaaaaagga aagaaagtta ttatacagta aaaaaagaaa ttattcttca
    16801 tgctagagat cagtacacgt tcgagcccaa tatcaaacac tagttccagg caggcgcaac
    16861 aggtgcggtt atcactgaat tttcttccag cagtgacgat tcaagtgata tttttacaaa
    16921 tccagctatt tatcgttaac aaaaagattt ttaggagaag ctgatgaaca aaaatgatca
    16981 aattttgatc gtttagttcg tcggcttttt tatttagaga aacatcattt atcagtgaaa
    17041 cataacacga gaacaaaatg attttttcgt taataaaaga aacaagtatt tgttgtttaa
    17101 aataatacac gttattggca tataggacga aatcccagta tcttctataa gtaagtcatt
    17161 tcatatcgat gaaaacacgt tatttatttt tgttttatct ataaaatggc tgaaaaatga
    17221 tttttttcta aatactatat atagtggcgt ctatcctata taccactaaa aaactacaaa
    17281 atctagtctt ttattttaaa aaaacacgtg tagaatgaag atagactttt gagttaggga
    17341 gtgttttttt atgaaactta ctgttttttg cggttcccgt tttggtaata aagaaagtta
    17401 caaatttatt gcgcaaacgt taggaaaata catggcacaa gaagaaatcg aactggttta
    17461 cggtggttca tactccggga ttatgggagt gattagtaaa acagtactgg aaaataacgg
    17521 aaaagtcaca ggtatctatc cgaatgggct ttttgaaact gaattgcctg gaaaagatgt
    17581 tactacattt attcctacag aaacgatcga tgaaagaaag gtattattat ttgaaaaggg
    17641 agatgctgtc ctcgttttcc ctggaggtct aggtacattg gaggaattct cacaacttct
    17701 ttcttggata gcaatcggtc tgacgcccga caagccaatc ggaatattga atattggcgg
    17761 ttattacagc ggcttgcaac aattattaga aacttttgca aaagaggatt ttatggataa
    17821 aaaatggttg gatcaagtaa tcttttccaa caatccattg gaactggtca atgaattacg
    17881 agcagtatct attaaaaatc agttattatt gaaggaggca aaataatgag tcaaatatat
    17941 ttagcaggac catttttttc agaggagcaa atcgatcgag taagtcgtat cgaaaaagcg
    18001 ttggaggaaa acaaaacagt cacaagtttc tactctccac gtcatcacca agaaagcaat
    18061 tatgaactat tcagcgctgg atgggcgcag gaagtatacg aaaaagatat ggaagaactg
    18121 acaaatgcag aatttgttgt tgctatcctt gactttgagc atcaaacaat cgatccagga
    18181 acggcttacg aattaggtgt ggcaactatg ttgaaaaaac cgatgatcat tgtacaagaa
    18241 gagacggtac ctacgaactt gatgattaca caaagtctcc atacgtattt aaaatcagac
    18301 caagctgtac gtgaatatga ttttgaaaca ttgcctgttg aaacttacgt aggcgagtac
    18361 ttatagaaga ggaagggttg tatgtcagtg attttgattt caggaacgat tggtgcaggt
    18421 aagagcagtt tgacggatat gcttgcaaaa gagattgatt ctaaaccatt ttatgaaaat
    18481 gtcgaagata acgaagtatt gccgctcttt tattcaaatc ctgagcagta cgctttttta
    18541 ttgcaaatct tctttttgaa caagcgattt ttagcaatga aaaatgcttt agtgaacgat
    18601 gacaatgtgc tagaccgatc catctatgaa gatagcctgt tgttccatct aaacgctgat
    18661 ttaggtcggg taacggatat cgaagtacag cagtacgata gcttattaga tacgatgcta
    18721 aacgagttag atgatgttgc tccaaaaaaa cggcctgatt tgatggtcca tatcaaagtt
    18781 tctttggaca cgatgctaga acgtatcaaa aaacgtgggc gtgactatga acagctagaa
    18841 agcgatgaga cgttatatac ctattatgaa acgttgaaca cccgctataa tcaatggtat
    18901 gaagattttg atgtatgtcc taaaatacaa gtggatgggg ataaattcga ttttgtagaa
    18961 aatccagaag atgcaaaaaa ggtgatccgt cagatccaag aaaaattagc tgaattggaa
    19021 ggaaagcctg ctggaaaata ttttacggat aaccagcatg caaaaggtca agaattaggc
    19081 taaaaataaa aaaccaaacg acgggaaaga aaaattacta cgtgatgtag aataagcctt
    19141 tctatcgttt ggttatttta tattcaaata tttgaaataa ataaatcttt actctttgat
    19201 ttgtaaactg aaatgatcca ctgtttgttt tactgatgaa gcttgatcaa aggaagaagc
    19261 tgagtttgaa gttcataaaa atcagccata gtgagttttg cttggatttg tttatcttct
    19321 tctacttttt acataatttt gaacaggata agattcaaat gttttaaatg aatctttgac
    19381 tagtttgtga cagttgtcgc atatgctcaa gcttccttgg tcattaaatt ctgtatggat
    19441 atttttgttg ttattacatg tacaataatc aggactatcg atcatccatt tcgtctcctt
    19501 tacaatcatc ttgcttcttt actatagcag aaaagaaggg gagaaacaaa agaagatcca
    19561 acagacggca ctatagactg tagatgttgc catcctcatc cttcaaacgg taattaccga
    19621 tttttgtaaa tatgactgtt gtttcttttt tattttcttt tcctgaaata atatattcgt
    19681 catctttggt agtgatcttg atatctgtca gcactgtttt ttcttcagat gattcagaag
    19741 aatcagttcc ttgtgtctga tacgtgcgtg tccattccat ttttttatct gtaaatgtca
    19801 actgtcccgc ggataaatcc atatctttac gtacttttag cgtatataca ctgttggtgg
    19861 tatctgcttt ttccgctttt aagacagaag aatcggttgt ttcgtccgag gattggtcag
    19921 agccgccaaa caaatcacag ccagtcaatg tcaaaagtcc cgcagacaaa ataaatagta
    19981 cgattaattt cttcaaaata cttcccccat ttgataggat aaagaaatta taccattgga
    20041 atgtaaaact tcaaacggat ggaagagaaa aacgcacaaa acacccacaa atagagtaaa
    20101 ttttagaata aaattttaaa aaggcaaaaa atccgccact ttcagaaagc gttttaaaaa
    20161 agtgttgcaa tcttttaaaa aaatgctata ttaaaaatga accggttcaa atacttgttt
    20221 aagtataatt aatagtgaga ggaagttgct ctactgaaga ctttagttat atggatattg
    20281 atgacaagca acactatgca aagaatgcgg tcatcataat cgaacaacaa gaaagagaaa
    20341 tcaaatgtaa ctggcaggag tgagaatgta tggcatcgat ccgtgatgtg gctaagatgg
    20401 ctggtgtttc agtgggaaca gtttcccgtt acttgaatgg tcagcaactg aaggaaaaga
    20461 acatgaaaaa aatagcagaa gcgatttcag cgttggacta taaagaaaac atcattgcta
    20521 aagggttgaa gaataatcga agcttctcta ttggactttt gataaacggg atttccagcc
    20581 gttttgggtc ggaagttgtc tcagggatcg aaagagtagc tgaagaaaat gggtatagct
    20641 tattattaag cgggtttgcc gatcagccag aaaaaattga acaaaaaatt gaatacctga
    20701 tgaagcatgt gatcgatggg ctgattgtct tcttatccga agaagagtgg tctggatttg
    20761 aaatgctcac aaaaatgagt attccagtta tcgcattgaa ttgtccagaa ggaccagcag
    20821 gtattgatac aatattagtg aatgatcgag aaagtgtagc aaaagtgatc gctcatcatg
    20881 gtgaacaagg acataaaaaa atcggattta ttgctgccac tcaaacagac tacgtagcaa
    20941 gagaacgttt agcaggagcc aaagaagcgg tccaaaatga tccatcgatc caattagagg
    21001 tctttacagg agactattct agaaaaagcg gctattacgg ggcaaaagca ttattagaaa
    21061 aaggggcaac tgctattttt gtcagcaatt acaatatgtc gattggtgcc atcgaatgtt
    21121 tcaatcaaga aggtgtcaga atcgggaagg atattgcgtt cagtcactat gattatttag
    21181 ataaaaacga tctgagtatc cttccgaaaa tcacgatcac gccaccaaca gataaaatcg
    21241 gggaacgagc agcagaacag ctgttaaaac gaatcaaaaa agaagaggaa gaaaatgaaa
    21301 aagtgattgt gatggaaaat atcatacatg gaatcaacta aaacgaacta aaaaagaaaa
    21361 agaggtgctt tgatgaaaaa atatcaattt ccagcaaatt tttggtgggg ttctgcagca
    21421 agtggcccac aaacagaagg aaggatttca ggcgatggaa aaggagagaa catttttgat
    21481 tattggtaca aaaaagaacc gcaaaagttc tttgatcaag taggacctga aaaaacctcg
    21541 caagtttata cgaagtatca agaggatgta cagttgatga aacagactgg gcataattca
    21601 tttcgaacat ctatccaatg gagccgtttg atacctgatg aatctggaac aatcaacgaa
    21661 gaagccgttc gtttctatca tcgttatttt gatgagttga ttgaaaacgg tatcgaaccc
    21721 tttgtgaatc tgtatcattt cgatatgccg atgtatctgc aagaaaaagg cggatggttg
    21781 aaccgagaaa cggttgaagc ctatgaaaaa tatgccaaaa gttgttttga attgtttggt
    21841 tcgaaagtaa aaaaatggtt tactcataat gaaccaatcg ttccagtcga agcaggttat
    21901 ttatatcgat ggcattatcc agaagagtca gatatgaaaa aagcgatcca agttggctat
    21961 catgaagcgt tggcttcagc gttagccatc aaatcttacc atgaaatgga gcaaggtggt
    22021 gttattggta tcatcttgaa tctgacacca agctatccaa gagacgaatc aaaccctgaa
    22081 gatgttaaag cagcgcaaat agcagatgcg ttcttcaatc gttcattcct tgatccagct
    22141 gtaaaaggcg agtttccaag tgaattgatt gagatagtaa aagaacttga tctcatgcct
    22201 gtgatccatg cagaggattt gacggttatc aaagaaaata cagtagatct tttaggtatc
    22261 aattattacc agccacgccg ggtaaaagca aaagaaacac caatcgatac taaaaacggt
    22321 ccaatgcctg aggattattt cgacaattat gaaatgcctg gtcgtaaaat gaatccttat
    22381 cgcggatggg agatctatga acaaggaatc tatgatattt tgatcaatgt acgagatcat
    22441 tatggaaata tcgactgctt tatttctgaa aacggtatgg gtgtagaaaa cgaagcacga
    22501 tttatcaaag aagatggcat gatccacgat gattatcgga tcgaatttgt ccaatctcat
    22561 ctgaaatatg tccatcaagc cattcaagaa ggtgccaatt gtttaggcta tcatatgtgg
    22621 acatgtatgg ataactggtc gtggaccaat gcatataaga atcgatatgg ctttatttca
    22681 gtagacttag cacaagatgg aaaaagaaca atcaaaaaat cgggtcattg gttcaaagac
    22741 gtatcagata aaaacggatt tgaggcttaa gaaagaatag ttgagagcaa atgcgtgaaa
    22801 ttctccattc ttaccatttt gtgaaaaaga agttcaatta tttacgatct ttttcaactt
    22861 ttttgtcttt ctatttcatt ttttgtatat aatagaatag tcaaaattaa ataggagtgg
    22921 aggaaagcat gaggaaaaaa agaccgtttc ttctcttttg gttattcggt tttttcttac
    22981 tgcttatttt atccgcagca ttttcaggaa aaatgacagc agatcttgct cagaaagaaa
    23041 cagaaaaaat cgtagcaagc aaagaatcag tagataattt caaaaaagaa ttctcaactt
    23101 attggtgggc ggagtataaa ataactgcca attacgaaaa aggcgaattg gacatttatt
    23161 tgccaaaagg aaaagaagaa aatcaattta ttaatatcga tgacgtactt agtgcgattt
    23221 ctctcagaca atatcaattt aaagacaagc atttgaccgt tttgcttttg actcatgatg
    23281 gcgacaaagt agcccgtttg aaaaatggaa aagtgatcac cgcttattct gaaaaagtag
    23341 acaaaaaagt ggtcgccgat cgtttagcgg aatggaaaaa gacaaatgaa caagtcaaga
    23401 aaacaactgt ctcttctcaa aaaaacgagt cgacaggaac aagtccttca gattctggca
    23461 gtggacaaac gataacggga agtgaagaaa atcaagcctc aattattacg aatgagtcat
    23521 cacaaggaca atatcaatcc tatggacaat gaaaaacaga ggttgtgaaa tcagcgtttt
    23581 gacctgagta ttagagaaac aaacggcaaa atagttcctc atattttgcc gttttttgag
    23641 ctaataccga aggtcagctg attgaacacc gtgtatcaag aggtcttgag cctgccgttt
    23701 aactctgagc cacaaggagc aatttcaaaa aatagctcct tatatttttc gtttggttgg
    23761 gcttattacc gcagatgcaa gcttttgaac accgtttacc aagaggttgt aaaattagcg
    23821 tcctgacata atctaatacc gagggtcagc tgattgaaca ccgtgtatca agaggtcttg
    23881 agcctgccgt ttaactctga gccacaagga acaatttcaa aaaacagctt ctcatgtttt
    23941 ataaatattg tgacttgtgg ccgaggagtt ggcaggagaa caccgtttaa ggaagaagtt
    24001 gtgaaaaagc tgtcctgcat caagaaataa ggagctgcga caagaatttt gtcacagcct
    24061 ctttttttgt acgaataccg agaaactggc ttttgaatac cgtggagtga agcggtcacc
    24121 acaagacttt tatctcttca ttagtttaat cgcttacatc gcagcggttt tatgctgtga
    24181 aaagtggacg gaataatctt tagaaagaat aaaaaaatct taagataaca acttttttag
    24241 gaaattttca tttttcagga gcaaaataag aaaaaatgtg aatagagaga agtgatatac
    24301 gtgacacaac ttaaaaaatt gtgccaaaat cgtttagcaa tcgttttgac agctgttttc
    24361 tttttctggc tgaagacgat cattgcctac tatgcagatt tttcattagg tgtggaaggt
    24421 acgatccagt actttatctt gtggatcaac ccaattgcga ctaccttatt gttttttggc
    24481 ctttctttgt atattaaaaa gccaaagcca gcgttagcta ttcttttgat cattgatatt
    24541 ttaaatacgc ttttgttgta cttgaatatt attttttatc gggaattcac agatttcatt
    24601 acagtcaaat ctgtattagg cttttcgaaa gtgtcgcaag gattgtcagg aagctccttt
    24661 tctttgatga agccccatga tgtcatctac tggctggata tcgctgtgtt cattgggcta
    24721 ctggtttggt tgaaggtcaa gaaaatacct atcaaaagca accctgtcgg caaaccgatg
    24781 gctatcgctg ttacctgtct ttcagggctt attttttcag gaaatctagc gctgagcgaa
    24841 gccaaccgtc cacaattgct gcagcgaaca tttgaccgtt catatattgt caaatatcta
    24901 ggaatcgatg cgtatactat ttatgacgga atcaaaacag gaatgactag cagtgttcgc
    24961 gcgcatgcaa gcagtaatgg gattgatgaa gttttagatt atacaaagaa acattatgca
    25021 gagccaaatc ccgaaacatt tgggatcgct aaagggaaaa atgtgatcgt tctccattta
    25081 gaaagtttcc agcagttttt gattaatatg aaggtagacg gacaagaagt cacaccgttt
    25141 ttaaatagta ttttccagaa tcaagcaacg atcagctttg acaatttctt tcatgaagta
    25201 ggacagggaa agacaagtga tgctgaaaat atgttggaaa ctggcacatt tggtttacct
    25261 caaggctcat tattcacaga acttgggtca gacaatgtct ttcaagccgc accagcgatc
    25321 cttggacaaa agcaaggcta tacaagcgct gttttccatg ggaatgtagc tagtttttgg
    25381 aatagagatc atgtatataa aaacttaggt tatgacaatt tctttgatcg atcttatttt
    25441 gatgaatccg acgaaacgtt aggttatggg atattagata aagatttatt cagagaatct
    25501 gcccaatatc ttgagcatct acaacagccg ttttacacga aatttctttc cgtaaccaac
    25561 cacacgcctt attatacgga tgacaagaat ttcgactttc catctttgaa tactggcaat
    25621 agcacagtag ataattatgt tcgaacggca cattatctgg atcaatcatt ggaacaattt
    25681 tttacgtatt tgaaaaaatc tggtatttac caaaattcaa tctttgtgat ttacggggat
    25741 cattttggga tctcgaatac agataataaa gatttagcaa gcgctcttgg taaagatcca
    25801 gatacatggg atgaatttga caacgcacag atgcaacgag tgccattgat gatccatatg
    25861 cctggttata ctaaagggac agtcaatcat gaatacggtg gagaaatcga tgttttgccg
    25921 acattactac atctattggg aatcgatgat aaagattatc ttcattttgg tacagactta
    25981 ttatcttctc agcatgatca agtagttgct tttcgaaatg ggaactttgt gacacccaaa
    26041 tacaccgtcc taggaggaaa agcttacaat aatcagacag gagaaatcat cgatacgaaa
    26101 gcagcaggaa tcgatgagga aatctccaaa gatcaggaaa aagtcaagac tgcgttaagt
    26161 ctttcagata agctgaatca ggaaaatctc ttgcggtttt atgtgccaaa cggatttgag
    26221 acgattgatc cgaaaaaata tgactataaa aatgaagcgg aacgtaatga agcaatcgaa
    26281 aagcaaaaag gcgaacattc gacaagtgtc ttttcaaaaa atggtaataa aacaacaacc
    26341 aacttatatc cagtaacaag aacagacgtt gaacagcaaa aaaataatca ataaacaatc
    26401 aaaccaacct tcagtagtta gacaacattc taatgcttga aggttggtat tttttatgaa
    26461 aaaaacattt ttttattttt ttcgtgtatt tttccttttt aacgtatcta tttat
//
LOCUS       DAZAID010000033        26082 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000033 DAZAID010000000
VERSION     DAZAID010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.033"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..114)
                     /locus_tag="ACG9BE_001268"
     CDS             complement(<1..114)
                     /locus_tag="ACG9BE_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3032021.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINE"
     gene            complement(228..320)
                     /locus_tag="ACG9BE_001269"
                     /pseudo
     CDS             complement(228..320)
                     /locus_tag="ACG9BE_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(456..1091)
                     /locus_tag="ACG9BE_001270"
     CDS             complement(456..1091)
                     /locus_tag="ACG9BE_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3032022.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            1603..2745
                     /locus_tag="ACG9BE_001271"
     CDS             1603..2745
                     /locus_tag="ACG9BE_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN3032023.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            2738..2944
                     /locus_tag="ACG9BE_001272"
     CDS             2738..2944
                     /locus_tag="ACG9BE_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFN3032024.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            3023..4216
                     /locus_tag="ACG9BE_001273"
     CDS             3023..4216
                     /locus_tag="ACG9BE_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3032025.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            4421..5560
                     /locus_tag="ACG9BE_001274"
     CDS             4421..5560
                     /locus_tag="ACG9BE_001274"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFN3032026.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5553..6353
                     /locus_tag="ACG9BE_001275"
     CDS             5553..6353
                     /locus_tag="ACG9BE_001275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN3032027.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6353..7180
                     /locus_tag="ACG9BE_001276"
     CDS             6353..7180
                     /locus_tag="ACG9BE_001276"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN3032028.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7185..7892
                     /locus_tag="ACG9BE_001277"
     CDS             7185..7892
                     /locus_tag="ACG9BE_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN3032029.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            7992..8858
                     /gene="rfbA"
                     /locus_tag="ACG9BE_001278"
     CDS             7992..8858
                     /gene="rfbA"
                     /locus_tag="ACG9BE_001278"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFN3032030.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            8872..9444
                     /gene="rfbC"
                     /locus_tag="ACG9BE_001279"
     CDS             8872..9444
                     /gene="rfbC"
                     /locus_tag="ACG9BE_001279"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFN3032031.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9466..10494
                     /gene="rfbB"
                     /locus_tag="ACG9BE_001280"
     CDS             9466..10494
                     /gene="rfbB"
                     /locus_tag="ACG9BE_001280"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFN3032032.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10592..11443
                     /gene="rfbD"
                     /locus_tag="ACG9BE_001281"
     CDS             10592..11443
                     /gene="rfbD"
                     /locus_tag="ACG9BE_001281"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFN3032033.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11477..13510
                     /locus_tag="ACG9BE_001282"
     CDS             11477..13510
                     /locus_tag="ACG9BE_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032034.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13554..14834
                     /locus_tag="ACG9BE_001283"
     CDS             13554..14834
                     /locus_tag="ACG9BE_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFN3032035.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15044..15850
                     /locus_tag="ACG9BE_001284"
     CDS             15044..15850
                     /locus_tag="ACG9BE_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3032036.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            15862..17082
                     /locus_tag="ACG9BE_001285"
     CDS             15862..17082
                     /locus_tag="ACG9BE_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3032037.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17072..18658
                     /locus_tag="ACG9BE_001286"
     CDS             17072..18658
                     /locus_tag="ACG9BE_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3032038.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            18697..20835
                     /locus_tag="ACG9BE_001287"
     CDS             18697..20835
                     /locus_tag="ACG9BE_001287"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN3032039.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21203..22186)
                     /locus_tag="ACG9BE_001288"
     CDS             complement(21203..22186)
                     /locus_tag="ACG9BE_001288"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN3032040.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22564..23955
                     /locus_tag="ACG9BE_001289"
     CDS             22564..23955
                     /locus_tag="ACG9BE_001289"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFN3032041.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            23971..25011
                     /locus_tag="ACG9BE_001290"
     CDS             23971..25011
                     /locus_tag="ACG9BE_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN3032042.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25030..>26082
                     /locus_tag="ACG9BE_001291"
     CDS             25030..>26082
                     /locus_tag="ACG9BE_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFN3032043.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTE"
BASE COUNT         8447 a         4402 c         5327 g         7906 t
ORIGIN      
        1 ttcattgata atatcttgca atgatgtgtt atcatatcct ttttcacaaa acatttttgt
       61 agaaacatct aaaatgagtt ttactgtttc ttctggatat ttatttcgtg ccataactta
      121 cctccaatac aaaccgttgg tctgattaaa gtatatctta gtattttatg cttgtcaata
      181 taaagacttg caattttata aaaatgcaga atgcaagcct ttttcattta ttctacaatc
      241 ttccagttgc tttccttatg cggtgtcaat tcatactgtg atacctgcgt tgcctttgcc
      301 tgatttacca caaatcagat tttatccctt tcttcctctt gattgcccac aacagaaaag
      361 aaaaagccct gtcaagagct ttgccaccat tggtggtgct ttgcactctt gatcgggctt
      421 tttgtttgct gtatcttctc gcaagaggaa aaaagttact cgtcctcatc atcaaaattg
      481 ttattcctta atacttctcg taacagttcc atatcttctt ttgagttcgg gtttatcatt
      541 ttcacgactt gataaggtgt cttatactta tgtccctcta tgctttcccc aaacatatcc
      601 atactgtata aatcatcata gcggtctaat aatttttgaa acttcaaatg ctggataaat
      661 tctttgattg ggtaaaggtc ggacgcttca atgcttttcc cgttgatata gtctgtaata
      721 tattcccgta acttttctaa ctcatattcc agccattctt cctcgctgtc aaagaagtta
      781 tcatagtgtc catgtttcag ttctgcattt agacgttctt cggctcttaa aaactgacag
      841 acttcctttt cagattgatt gacatacttc cattctcccg acaacaattc attgggcgta
      901 acgtccagca cttccattat cttttccagc gtttcatagg caggatagtt caaccctctt
      961 tcaatcttgg aaaggctctg catattgata ccgattttgt ccgcaagttc ctgttgtttc
     1021 attcccctaa attttcttat ggtctgtata ttatttccta aatggcttat tttcattgca
     1081 taaccctcca tatctcgtta tatcttcgct attataagtc tatcatgctt aatatgattt
     1141 gtaaaggcaa aaaaagatat tgacaaataa gcatataaga attactatgt tcttgtaatc
     1201 gaataagcac aatagaatga aatataaaaa cattcaaaaa gaaataccta tctgattaaa
     1261 tccgtagata tgaatatcgg ttgaaatgga acttgttaga aaaggcaaaa cagaacttgc
     1321 tgacaaagta tttctgacac ggtggcggaa gcctctcagg cttaccgcca tgacaccttg
     1381 tgtcaagggg aagaaatgct ttgtcggctg gcgagcctgc gagccttgtc tgtgcccccg
     1441 ttatctaaca ggggggcact atttcacaaa agacaagtca aaaaccatac acccccaaac
     1501 ctgtgagtag taatgagagg ttttaagaag atagtacata tcacactttt tgaaaggtgg
     1561 tttacaggta tttcaaggct attgtgggat tggaggaata caatgaacaa tttagaattt
     1621 gcacttgaaa tgaagaacaa acgtcttgcc agcggtcttt cacaaggaga acttgcaagt
     1681 cttacccatg tatcaagata tagtatcaac cgctttgaga acggaaaagc taacgccagc
     1741 aaggaaacac agaacattat cctgcgttgt ctgaattact atatctgcga taagcctttt
     1801 tatctgctcg ttgattatct gtctgtccgt tttccaacga ctgacgcatt ggaagttatc
     1861 cgaaaggtgc ttggcatgaa agcagattat tttatccatt atgactatgg gtattatggc
     1921 tataaggagc attatgctta cggggaaatc aaggtcatgg cttctgatga tgaacacatg
     1981 ggcgtatttt tagaattaaa aggggcaggc tcacgcaaca tggaatatgt cttacaggca
     2041 cagaacaggg actggtattc attcttaaac cgctgtcttg actgtggcgg tgttatccga
     2101 cgttttgact tggcaatcaa tgatatgtgc ggtctgctgg atattcctgt gctatctgaa
     2161 aaatacagaa atggtggtgc ggaatgtcgc tgtaaaaact atgaaaatgt acaaggtgga
     2221 aaattaagcg gaaaaaaccg taatttagca agtacccttt atatcggctc aaaggcaagc
     2281 acaaaatatt tctgcctgta tgaaaaacaa aaggaacagg caacgaaaaa gaaacatacc
     2341 gatattatca accgttttga aattcgtctg cgtgacaaaa aggcggtaca ggcagttgaa
     2401 gaactgttat tgacgtataa ccctcatggg ctggtgtttt atctcattac cgattttgtg
     2461 gaatttcctg actatccatt gtgggagata ttcatttccc atgacagttt accttttgaa
     2521 atgaaccctg tacctgtcaa tatggaacgc actctgcaat ggctggaaag acaggtcatg
     2581 ccgtcaatcg tgatgataga ggaaatcgac aggctgacag gttcaaacta catgaaaatg
     2641 attgatgaat gtacccacct ttctgaaaaa caggaaatgc ttgtggaaca gatgtgtacg
     2701 gacatatcag acgtaatcga aagtgagggg gtgttttatg agtaatacac aggatattcc
     2761 cgtatgggaa aaatacaccc ttaccattga agaagcgtca aagtatttcc gcattggaga
     2821 aaacaagtta agacgattgg cagaggaaaa caaggacgct ggctggctca ttatgaatgg
     2881 caaccgcata cagattaaac gccgacagtt tgaaaaggtt attgataaat tggacgcaat
     2941 ctaatgcaaa tgagccttgt atgtgttatg atgaacacaa gtcatatcaa ggctctttcc
     3001 aacaaggaaa ggagcagaca ccatgaaaga aaaaagacgg gataacaaag gtcgtatcct
     3061 gcatactgga gagagccaac gaacagacgg aaaatactta tataaatatg tagacgcatt
     3121 gggaaacaca aaatatgtgt atgcttggag attgacaccc acagacccga caccaaaggg
     3181 aaaacgggaa aaaccctcac ttcgagaact ggaacagcag ataagacggg atattgagga
     3241 cggtatcgac agcacaggca agaaaatgac gctttgccaa ctctatgcca aacagaacgc
     3301 acagaggaca aatgtgaaga aaagcacaca gaaacaacgg gaacaactca tgcggttatt
     3361 aaaagaggac aagttaggtg ctaggagcat tgatacgata aaaccctctg acgctaaaga
     3421 atgggcgtta cgcatgaaag acaaaggctt ttcctacaat accattaaca accataaacg
     3481 ctcgttaaaa gcgtcattct acatagccat acaagatgat tatgtgagga aaaacccttt
     3541 tgatttcaag ctaagtgaag tcatagaaaa tgataccaaa gagaaagtcg cattgacaga
     3601 ggaacaggag caagccttat tgtcatttat taagacggac aacgtgtatc acaagcatta
     3661 tgatgatgtg ctgatactgt taaagacagg acttcgtatc tcggaactgt gcggactgac
     3721 agtagccgac attgatttca agaatgaagt tgtgattatc gaccaccagt tactaaagag
     3781 taaggaacag ggctattata ttgaaacgcc taagacgaaa agcgggataa ggcaagtgcc
     3841 attaagcaaa gaaacgatac aggcatttca acgggttatc aacagccgac caaagataaa
     3901 gtcgatagag gtagacggac acagcggttt tgtgtttatc aatccgaaag gtaatccaaa
     3961 agttgccatt gattataacg ccttatttat ccgtatggta aagaaataca acaagcacca
     4021 caaggacaat cccttgccac atattacacc gcatacgcta cgccatacat tctgcacaag
     4081 gctggcaagc aagaacatga acccgaaaga tttacagtat atcatggggc attcaaacat
     4141 cagtatcaca atgaactggt acgctcatgc gtccatagat accgcaaaat cagaggttca
     4201 gcgtctaatc gcatagaagt atttaccacg attttaacca cgattgacag ggaaaatata
     4261 agaagataga cctagatatg cgaggtttac cacaaaagca aaatgcccgt agagccgata
     4321 aaataaggct ttgcggacat ttgagaagat ataaaaagat agtcaaaaag acatatataa
     4381 tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt cttttagtat
     4441 ggttataaga ttttttctga caattttatt atcactgata ttaacacctg tattcaaaat
     4501 catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa ataaagttcc
     4561 aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga ctttattctt
     4621 attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt ctttagtaat
     4681 catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa aaagtatagg
     4741 gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg attccttcac
     4801 gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa cgattttttg
     4861 gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc tggcggcagg
     4921 gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc tccatgcttc
     4981 tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg gtttttttcc
     5041 ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat tcctagggtt
     5101 tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt catcgatttc
     5161 tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca ttaggagaaa
     5221 agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc gactaatcag
     5281 tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt tgatgttttc
     5341 tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat tgattattgc
     5401 catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg agaaacatca
     5461 gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc aagtaaaaca
     5521 gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac cagccgatcc
     5581 ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat aattttattt
     5641 caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat tcacttcccg
     5701 agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc tattttcatg
     5761 atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc gattctcgct
     5821 atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca gcattggtct
     5881 tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt aatgaagatg
     5941 gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt ttgcgctttg
     6001 tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt gaatgccgag
     6061 gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt atggtagctg
     6121 atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg tactttgaag
     6181 acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac acaccctttt
     6241 atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg tttcgcgtgt
     6301 ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc tgatgaaacc
     6361 agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc cagcttatgg
     6421 accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg ataatagtcc
     6481 tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc atgatccgca
     6541 aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg aaggaacacg
     6601 ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg aactattgcg
     6661 taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt ctgggagccg
     6721 acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag cagtagaagt
     6781 tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct ggtctgtcaa
     6841 atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt tggatcattg
     6901 gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg tacgaatgga
     6961 acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc gttctatttt
     7021 acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca gtcaccgacg
     7081 acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc atatctggcg
     7141 gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt tcggtttgta
     7201 tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt ttatctcagc
     7261 tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat acatgggata
     7321 tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg cctcgtcaag
     7381 gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta atctttttgg
     7441 cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac tattttgagc
     7501 agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc gagctgcaag
     7561 agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt aataatgtga
     7621 taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa cccgatattt
     7681 tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg ggaggaaaga
     7741 agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta aacgcgagtg
     7801 agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat ctgattacgg
     7861 cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc ttaatatttc
     7921 catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat caaaaaggag
     7981 agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt ctgtaccctt
     8041 taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg atttattatc
     8101 caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca acgccacaag
     8161 atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg cagatcgaat
     8221 atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga gaagaattta
     8281 ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt ggcggtctgt
     8341 ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt ggctatcatg
     8401 tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa gctttgtcga
     8461 ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt tatttttatg
     8521 acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga gaactagaga
     8581 ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa cttatgggac
     8641 gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca tctaccttta
     8701 tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa attgcttacc
     8761 gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg aaaaaaaatc
     8821 aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca gatgaaagta
     8881 acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg cgatcatcgc
     8941 ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt gaaatatgac
     9001 tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg gcttcatttc
     9061 caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc agttttagat
     9121 gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg atttatctta
     9181 tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg attcgttact
     9241 ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc agaagcagat
     9301 gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga tccatcaaga
     9361 gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga agcagaaaat
     9421 ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa aaacatcatt
     9481 gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt aaacaatcat
     9541 ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa agagaacttg
     9601 gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga tgcagaatta
     9661 gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga atcccataac
     9721 gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg tacgtataca
     9781 cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac tgacgaggtt
     9841 tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga aggagaaaaa
     9901 ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa agctggttca
     9961 gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat ttcaaactgt
    10021 tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca agtaacgaat
    10081 atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg tgactggatc
    10141 catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag aattggcgaa
    10201 acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga attgatctta
    10261 gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc aggacacgac
    10321 ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa gcctgagttt
    10381 acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca tgaagactgg
    10441 tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca ataatacaga
    10501 gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc tgtctctttt
    10561 ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag gaaatgggca
    10621 actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg tttcaacaga
    10681 tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca cagaattaaa
    10741 acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag aagaagaagc
    10801 aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac ttgcagcaaa
    10861 agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg gaaaaaacaa
    10921 aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg gtcgtacgaa
    10981 attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca tacgaacttc
    11041 ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc tagcggaaac
    11101 ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga cacgtacttt
    11161 ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt atcatttgtc
    11221 gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag atacagatgt
    11281 agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc cgcagtattc
    11341 agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt ggcaagaagc
    11401 acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt ttatcaaata
    11461 gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag aaaagccaaa
    11521 agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc gctatcttat
    11581 tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt cacttggtgt
    11641 ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga tttttggaca
    11701 gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc tctcacaagc
    11761 tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa acatgatcat
    11821 tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca actggggatt
    11881 ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat tagtcgtcat
    11941 ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag ctctatcgct
    12001 aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca tgcaacatgt
    12061 gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt atttctacaa
    12121 tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt tcggtattgg
    12181 gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc tgatttttta
    12241 ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt tggatgtagt
    12301 gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct ggctaacgtc
    12361 aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg taagtactgg
    12421 aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa ttccttttga
    12481 agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt ttccaactgt
    12541 attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa agttactggg
    12601 acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg ggttgccaaa
    12661 atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca tccttacttt
    12721 tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga gagaagatgt
    12781 tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt atttctatgc
    12841 gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg cagttgctgt
    12901 gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt tttacctcgc
    12961 ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag aaggtacagg
    13021 agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac aagatcctgg
    13081 taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg gagtgaaatc
    13141 cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg atccgaaaga
    13201 agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg ctaaggaaga
    13261 tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt ttgaagacgc
    13321 agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt acaaccaatt
    13381 atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata tgatttttaa
    13441 agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa atcagctagt
    13501 cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg agagtggaga
    13561 taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc ttgcttttgt
    13621 tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca actttgttta
    13681 tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc attttaaatg
    13741 tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc cttttcattt
    13801 tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta ctgtttcctt
    13861 tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg gatcgcggta
    13921 tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta tttacagaga
    13981 tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct gatatttgga
    14041 tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc agtggatttg
    14101 ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt gttgcagctt
    14161 ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt gtttttgcga
    14221 tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt attgcttaca
    14281 cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct gaaaaagtca
    14341 atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta ggagaaaaat
    14401 ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac caatttttag
    14461 gaaaccacaa tagttataat tctattttga atatctatgg attgatagga tttgggattg
    14521 ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat ttatccaacg
    14581 ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt atggaggata
    14641 cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg gccgttcatc
    14701 gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca tatgacgata
    14761 tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct tccaggatcc
    14821 aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc gcagcctttt
    14881 ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag aaaatcttgt
    14941 atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata tggtaaaatg
    15001 gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta aagatttgta
    15061 tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt cgatcaatga
    15121 cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta tcaatccgct
    15181 atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac aaatgatcga
    15241 tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt tcttcttctc
    15301 agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag taaagaaagt
    15361 cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt acacccactt
    15421 attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc cttcagcaat
    15481 gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag gattgacttg
    15541 gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta cgatcgtgct
    15601 gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg attcaggtct
    15661 tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc gtgagtcatt
    15721 tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct gggttttcac
    15781 gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac atttttcaga
    15841 tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga tatcacaaaa
    15901 tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa tccttttaag
    15961 aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat aaaaaaaggt
    16021 gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt gaaaatcatt
    16081 acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat ctcagctttg
    16141 ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat ctatttaaat
    16201 ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga tatcattgaa
    16261 tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag cgggatgttc
    16321 gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat tgtggatgaa
    16381 gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt tacagaaatc
    16441 aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat tcgtcgtttt
    16501 tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga tacgatggaa
    16561 gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt ggatcggaat
    16621 aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa agcggaattg
    16681 ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact atacgttaaa
    16741 gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat tgcgctacga
    16801 acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat caagattcct
    16861 tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt gttagctggt
    16921 gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt ggtatataag
    16981 acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat cgtggtctta
    17041 gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa tggacctaga
    17101 tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta aagttttaga
    17161 gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac aatgtgaagt
    17221 gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg ctcaagatgg
    17281 attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac agacatttga
    17341 ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa cattgaagaa
    17401 agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta acttggcgca
    17461 taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt atggtctcct
    17521 agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt ttgaaaaagc
    17581 gggattattc attaatattg aagattattt gtacttagct gtaggtgata cagaattaaa
    17641 atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc gaccatttgg
    17701 tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa gttccattgc
    17761 agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa cgaaacaaga
    17821 tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga cactaagttt
    17881 ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac ctagctttat
    17941 cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga atgctgtagt
    18001 caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt tcgttctaac
    18061 ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg gggaattgac
    18121 accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca caaagcagct
    18181 ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg aaaatacaag
    18241 actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg aaagtgatga
    18301 atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa aaatccaaga
    18361 aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa aaatattaaa
    18421 aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata agttatcagc
    18481 tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca atcaagcaga
    18541 acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg agcaagaacg
    18601 ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg aaatgtaaat
    18661 aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga aattgccgta
    18721 tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt gggctgggca
    18781 attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa tgtgattgct
    18841 gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga tgttaagacc
    18901 caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat cttagacttc
    18961 caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata cccttatgat
    19021 gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg gataaattat
    19081 gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt gaagccagga
    19141 tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa agagcaaagg
    19201 aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc tgtctataac
    19261 gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac gtatgatcat
    19321 tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa gtgtctggaa
    19381 tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa tggacacatc
    19441 agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc actcttagat
    19501 aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct aaacgtacat
    19561 ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg caatcgcttt
    19621 gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa ttatatctct
    19681 cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg taaaggctat
    19741 gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc tgcagatcat
    19801 atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc aactgcaagt
    19861 aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac agatgccctg
    19921 tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt ttatgagatt
    19981 gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa gaatggctat
    20041 gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc aaattatgaa
    20101 atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt tgcagaatac
    20161 aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt caattattct
    20221 cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt cttgaataac
    20281 gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc acagtttgat
    20341 cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca gcatgcagga
    20401 gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga tcgaacacat
    20461 tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac tgcagcttgt
    20521 atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac attagaagta
    20581 gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta caatgtctat
    20641 gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga agatactcca
    20701 gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg gccagcatat
    20761 attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg cgacttttcg
    20821 ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc gcacataacg
    20881 gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa aataagtcga
    20941 ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa attgcttttc
    21001 aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca ccgtttattc
    21061 gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt attcatgctc
    21121 agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct gtcaaagaat
    21181 cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat tcctctacaa
    21241 aagccactct atcaattatc atgggtttaa aataagaagt gatggagata accagattgt
    21301 tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat atttcgttct
    21361 cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct tctaaagcac
    21421 tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt tttcctttat
    21481 acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc gataaacaca
    21541 aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg gttttaggag
    21601 acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa ttgacatggt
    21661 ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt gttagcttac
    21721 ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag ttgaattcgc
    21781 cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa tcttcacttg
    21841 aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt gttagcaagt
    21901 gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac tctggaaaat
    21961 aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca atgccaatca
    22021 gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct tttttataac
    22081 cattccattc atctgtataa acttctaccc catccttata tgcgacaatt tgacatatat
    22141 ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga tattacctca
    22201 ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga ttcttcacgt
    22261 aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc tgaaaaatat
    22321 aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc tttaatttac
    22381 caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt gtcttgatta
    22441 gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg cttataaact
    22501 aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat taggagtgta
    22561 tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat attaatggat
    22621 gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg tggagagatc
    22681 ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt gatttttatt
    22741 gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag tgacatcgtg
    22801 tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt gacttttgcg
    22861 ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct cggtacgctt
    22921 ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag tcgagataaa
    22981 aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa ttttggttca
    23041 aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta tgcaaacatc
    23101 aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga agaaactgaa
    23161 aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt gaatacaaca
    23221 tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga aagtattatt
    23281 gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt gtttgatatc
    23341 atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac agcaatttta
    23401 gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac aaaaaatcaa
    23461 aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc aaaatctgga
    23521 ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt tattcgagct
    23581 gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat gtcgatcgta
    23641 ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa cccttattac
    23701 tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta tggaaaatat
    23761 gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa atattcattg
    23821 atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa aatgtcctct
    23881 caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt tccgaatgaa
    23941 aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag cataatcgta
    24001 ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa agaattggat
    24061 tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac acaggaaatc
    24121 attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac caatcaaggg
    24181 cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct cttattcttc
    24241 gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt gctagaaaag
    24301 agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga cgagggaaga
    24361 atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca cgaagagttt
    24421 ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg gaacaaatgc
    24481 tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa tagttgtgag
    24541 gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat gaacccaaaa
    24601 gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata ccgacctaac
    24661 aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga cgatattaat
    24721 aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt ttctatttat
    24781 agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat attgagtgat
    24841 ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa aaaaataaca
    24901 aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt tggaagcttc
    24961 gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta gttaatggag
    25021 gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg tggagcagag
    25081 tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt tgattttttg
    25141 atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag attaggaggc
    25201 cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat agaagtgaat
    25261 aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt tggcggggga
    25321 attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca tagccatgca
    25381 actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt tttaaccaaa
    25441 ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg tgaaagtctg
    25501 tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct ttatagtaaa
    25561 aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag tacttttgtt
    25621 gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt agagattttt
    25681 ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga agggtcactt
    25741 agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt aaaattttta
    25801 ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt aatgccttct
    25861 ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt aaaattagtc
    25921 ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt tgtatcatta
    25981 gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc taataaaata
    26041 acacaagaac taaagagtta tgatgtttcg tacactactg aa
//
LOCUS       DAZAID010000034        25995 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000034 DAZAID010000000
VERSION     DAZAID010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.034"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG9BE_001292"
     CDS             complement(<1..1835)
                     /locus_tag="ACG9BE_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN3032044.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG9BE_001293"
     CDS             complement(1832..2536)
                     /locus_tag="ACG9BE_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN3032045.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG9BE_001294"
     CDS             complement(2548..4857)
                     /locus_tag="ACG9BE_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFN3032046.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG9BE_001295"
     CDS             complement(5052..5516)
                     /locus_tag="ACG9BE_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032047.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG9BE_001296"
     CDS             complement(5516..6142)
                     /locus_tag="ACG9BE_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN3032048.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG9BE_001297"
     CDS             complement(6149..6514)
                     /locus_tag="ACG9BE_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFN3032049.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG9BE_001298"
     CDS             complement(6504..6842)
                     /locus_tag="ACG9BE_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032050.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG9BE_001299"
     CDS             complement(6832..7158)
                     /locus_tag="ACG9BE_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN3032051.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACG9BE_001300"
     CDS             complement(7139..7417)
                     /locus_tag="ACG9BE_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN3032052.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACG9BE_001301"
     CDS             complement(7419..8783)
                     /locus_tag="ACG9BE_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN3032053.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG9BE_001302"
     CDS             complement(8796..9365)
                     /locus_tag="ACG9BE_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFN3032054.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG9BE_001303"
     CDS             complement(9337..10524)
                     /locus_tag="ACG9BE_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN3032055.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSSYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG9BE_001304"
     CDS             complement(10588..12315)
                     /locus_tag="ACG9BE_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN3032056.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG9BE_001305"
     CDS             complement(12312..12764)
                     /locus_tag="ACG9BE_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFN3032057.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG9BE_001306"
     CDS             complement(12876..13256)
                     /locus_tag="ACG9BE_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFN3032058.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG9BE_001307"
     CDS             complement(13253..13639)
                     /locus_tag="ACG9BE_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFN3032059.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG9BE_001308"
     CDS             complement(13641..13919)
                     /locus_tag="ACG9BE_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032060.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACG9BE_001309"
     CDS             13967..14695
                     /locus_tag="ACG9BE_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFN3032061.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14826..15302)
                     /locus_tag="ACG9BE_001310"
     CDS             complement(14826..15302)
                     /locus_tag="ACG9BE_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFN3032062.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15262..15618)
                     /locus_tag="ACG9BE_001311"
     CDS             complement(15262..15618)
                     /locus_tag="ACG9BE_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032063.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15596..15781)
                     /locus_tag="ACG9BE_001312"
     CDS             complement(15596..15781)
                     /locus_tag="ACG9BE_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032064.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15782..16054)
                     /locus_tag="ACG9BE_001313"
     CDS             complement(15782..16054)
                     /locus_tag="ACG9BE_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFN3032065.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16051..16251)
                     /locus_tag="ACG9BE_001314"
     CDS             complement(16051..16251)
                     /locus_tag="ACG9BE_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032066.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            16379..16591
                     /locus_tag="ACG9BE_001315"
     CDS             16379..16591
                     /locus_tag="ACG9BE_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032067.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTREKELNLEKLIIKKK"
     gene            complement(16624..16818)
                     /locus_tag="ACG9BE_001316"
     CDS             complement(16624..16818)
                     /locus_tag="ACG9BE_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032068.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16831..17013)
                     /locus_tag="ACG9BE_001317"
     CDS             complement(16831..17013)
                     /locus_tag="ACG9BE_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032069.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17010..17150)
                     /locus_tag="ACG9BE_001318"
     CDS             complement(17010..17150)
                     /locus_tag="ACG9BE_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032070.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17640..18005)
                     /locus_tag="ACG9BE_001319"
     CDS             complement(17640..18005)
                     /locus_tag="ACG9BE_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032071.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18045..18347)
                     /locus_tag="ACG9BE_001320"
     CDS             complement(18045..18347)
                     /locus_tag="ACG9BE_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFN3032072.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18347..18646)
                     /locus_tag="ACG9BE_001321"
     CDS             complement(18347..18646)
                     /locus_tag="ACG9BE_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032073.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18639..18803)
                     /locus_tag="ACG9BE_001322"
     CDS             complement(18639..18803)
                     /locus_tag="ACG9BE_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032074.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18809..19078)
                     /locus_tag="ACG9BE_001323"
     CDS             complement(18809..19078)
                     /locus_tag="ACG9BE_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032075.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19090..19839)
                     /locus_tag="ACG9BE_001324"
     CDS             complement(19090..19839)
                     /locus_tag="ACG9BE_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3032076.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19836..20528)
                     /locus_tag="ACG9BE_001325"
     CDS             complement(19836..20528)
                     /locus_tag="ACG9BE_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFN3032077.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20534..21205)
                     /locus_tag="ACG9BE_001326"
     CDS             complement(20534..21205)
                     /locus_tag="ACG9BE_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFN3032078.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21198..21539)
                     /locus_tag="ACG9BE_001327"
     CDS             complement(21198..21539)
                     /locus_tag="ACG9BE_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032079.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21717..22415)
                     /locus_tag="ACG9BE_001328"
     CDS             complement(21717..22415)
                     /locus_tag="ACG9BE_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFN3032080.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22502..22972
                     /locus_tag="ACG9BE_001329"
     CDS             22502..22972
                     /locus_tag="ACG9BE_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032081.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(23015..23194)
                     /locus_tag="ACG9BE_001330"
     CDS             complement(23015..23194)
                     /locus_tag="ACG9BE_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032082.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(23181..23519)
                     /locus_tag="ACG9BE_001331"
     CDS             complement(23181..23519)
                     /locus_tag="ACG9BE_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFN3032083.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(23535..23738)
                     /locus_tag="ACG9BE_001332"
     CDS             complement(23535..23738)
                     /locus_tag="ACG9BE_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3032084.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(23753..24529)
                     /locus_tag="ACG9BE_001333"
     CDS             complement(23753..24529)
                     /locus_tag="ACG9BE_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFN3032085.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            24828..25148
                     /locus_tag="ACG9BE_001334"
     CDS             24828..25148
                     /locus_tag="ACG9BE_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3032086.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            25166..25597
                     /locus_tag="ACG9BE_001335"
     CDS             25166..25597
                     /locus_tag="ACG9BE_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFN3032087.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            25727..>25995
                     /locus_tag="ACG9BE_001336"
     CDS             25727..>25995
                     /locus_tag="ACG9BE_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFN3032088.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFT"
BASE COUNT         7339 a         5351 c         4034 g         9271 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc tagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta cgaacattta gtgaacagtg
    14821 taattttaga ataaagagag ttgttctttg tacttctcgc tattgtaaaa atcaatttcg
    14881 ttttcttttt tcaatcggtc agcttgtctg tgttcatact catctaaaaa gttcaatgtc
    14941 tttctaattt ctgcatgtcg ctgtctgaca tacgatgagc tatacccaac agattcagca
    15001 atatcttcta agctcatttg atcaacatat ttcatcttaa caatttgatt atcgatacca
    15061 gaaaagctat ctatcagcaa taacatttct tctttttgct cgatcaacaa ttccaattca
    15121 ttttctatct tttggatatt ctcttctaaa gaagaagttc tcgaattctt ttcgatgcgg
    15181 acgtttgcta aatcaccaca gacccatcga ttcaattcaa gcttactttt attaagattc
    15241 cactttaagt aaagaatctg ctcatctagt tcttggtaat cttttaacca atgaaatctc
    15301 acaaacgcca ccccttatgg tagaatagta ttgcgctgct atctcgaaag gggtagcttt
    15361 tttatttttt cttagccgtt ccattctgga ttgccttttg ttccaaacgt cgttttttct
    15421 tcttaatctt tgattttgtt tttcccatac aattctcctt tccgaaaaca cattttgttc
    15481 gatttttaat actaaataaa agttggccag aatttttcgt tcgtctttac tcatcttttt
    15541 ttgaataaga ttactagcat aaaaatagtt agtaccacga aaatgaacag gtaaatcaat
    15601 tacgttcacc ttcttcatgc cagttatcag ccactagaat ctttgcgtta gtgccgccac
    15661 cgttttcttt tttgtatttt tcaaatgctt tataagcagc tgtttttgat ttatagattt
    15721 ttccggaacc gactgggaac atccatgtgc cctctgtgat agctaagatg tatactttca
    15781 tttaattcac ccctttaatc cccaacacta catatccgga cttctgcatg taatcagtaa
    15841 tataagtgat ctctgctttg tatgagtcac cagtgtaact aaatgttggt ctcgttagtc
    15901 caggtaattc atgtttaatg tattttttat attcacgtag tattaactga tcaccaactt
    15961 tgaagtcacg atcattcttg cggatctcga attgtttgcg tccgctcgtc actgcttcaa
    16021 aatattctgg tagtattttt agttcgtgga tcattctgtt tcctccttca aaattcgctc
    16081 atcatcaaaa agtgaatctc cacaaaatcc ttctcccaaa tatttatcgt tcatgtataa
    16141 gtcaaccaaa tatttgtgat ctgcttctcg atattcatta atattaacga tctttatttt
    16201 gtaaacatta ccttcgtcgc tcactatctt gaattgttct ctgactttca ccaccgttcc
    16261 tcctcttttt gaaaaaattt tagtgttttt gtaagacccc ctaaatcagc ttttttattt
    16321 gatataatac ttgtatcgat taatcaaatc gaaattaaat ttaaaaaggt gatttatcat
    16381 gaatatcaac aatttagtaa aactcgttga acaacgtttt aaagaatcag gattagaaca
    16441 attaaaaaaa gataattctc cagaattatt ttctaatttt accgaagacg acattcctac
    16501 tcttaaacaa ttaaaatatt attctgatta ttggacgcaa actcgtgaga aagaattaaa
    16561 tttagaaaaa ctcattatta aaaagaaata gcaactccag cccacacggc tggtttttta
    16621 gtttcaactt ccctccttac gggtcaaaat catcggttcc ggcataatct gatcatcctt
    16681 tagcaacaaa tcaggatatt ttttccttag cttcttattg ttcgtgtaag ttttcaatag
    16741 ccattggttg gcttcgcttt tgctttctcc agaaaaataa attttccctg tttggatgcc
    16801 tgcaagttga atcatcattt tcacctcaaa tcaaattttt tctgctagat ggagcaaaag
    16861 caatccgaca tcaagaatct tttgacccat tatttcagct actttgctcg gcttaactcc
    16921 ttgagaaaag agcaagcgtg cttgctctat ctcttcgtga gtccaaataa atttggcttc
    16981 ctcaaggata attactggat cagttgctct cattgttcct ctggaaaaat gatgtcattc
    17041 ttatgcttgg accaggtatc tgcatatgga acaaagtatt gtctagctaa ttcgatttga
    17101 tcaattaaag cagtttctga taattcatgg tctgcagcaa tttcattcat actatcgcct
    17161 tcatctaaac agataagacc gttgacgtta tcgattttta catttgtgac tgcttgactt
    17221 tctccacgct cataatattc tacagttgat actgatacat cagcttcgga gtttaattgc
    17281 agaatctgtt actgccggga taatttaata ttcttttcat aattcacctt ttttcctact
    17341 gacatcgaat atttttcatg ttatgattag ccgtgaaagg agagctatat tatgtgttta
    17401 cacatgatgt ccttagctca ttcggttctt tggaagcttt tttggaaata actctcaggt
    17461 tcttgacgca aatctcattt attctttgaa acaaaaaact tccattttcc gagagctttg
    17521 gatcacttca aaagagattt gcatttatat ttctgtgacc ttgagtcgcg taaagaacct
    17581 tacagcaatg gcggcaattc cactttcagg aaaccgcctt ttttcattga tattggtggt
    17641 tagcggacta cccattttct ttcggcagtg ccaaaccaca aatgatctgt gttgatgttt
    17701 aggtagttgt ataccagata agctaaatcc aaagtgcctg catcttcaag acgacaatat
    17761 tccaaaaatt ctcccatagc tggacataaa aatcctggag ctatcatatc ccaacagtta
    17821 atagtgcaaa gaaattcgtt tagcttctga acgttttctt tggtttcacg aattacaaca
    17881 tactccaatt cttctccgct ggcagagcat tccaaaactt caaattgatt aagaaatggt
    17941 agaacatatg cttcaaacat caacattcca tctttctgtt tttgcacttg ttttgcatca
    18001 atcatgtcta tcctcctatt cagttatttc ttccattaat ggaattattt caggtcactg
    18061 gacttaacga aaacaccatt gaccatcttt ccagtccgac ctttgatttc gtcataagca
    18121 aaatccaagc actcgtataa atccatatca ttttgcattg ccaagatgat caaggttact
    18181 actacatcgc caattccgtc tcttaaacca tgttcatctt ttctagctag agaagcggca
    18241 acttccccga tttcttcgac cgtttttaac atttgcttgc tgaaatccgc ttgatccaat
    18301 cccttatctt ttgcccactg ctctactttc gcgattagtt cgtccattat ttctcctcca
    18361 catacctaaa ctgtcttcct tttgaatcaa tccataagct cctagctcta tcccaaataa
    18421 tatttttgct cagaccagta atttcagata actgtttagc ggtacccgtt actagaattc
    18481 gatcaccatg ccagattgca atctttctcg gcgttctccg tttgggcttt tcagtccaca
    18541 ttgatttacc gagcttttgg acttctgcaa ctattttttt atcttcctgc caatcttctg
    18601 aataagtcaa ttcgataatt cgctgcattg ccgctttctt atccatcccg acattctcct
    18661 ttcagtaatt tgagtacttg atcaagtgcg ctctcacgtc cgccatgaaa tgtgttgagc
    18721 cacttgtctt cgtacgacac actttgtctt aaagcttctt gatgcattaa ttcgatctgt
    18781 gctgtaaatg tctttaggtc catcttgttt acacctgctc aagttcatta agatgttttt
    18841 gcaagccttt aacacaatca atgaatagta atttggtata tgctaaattt cttaattgcg
    18901 ttccatcgat atagagtgcg aaatagtatc tgagtttact ccaactcgaa cgatcattct
    18961 taattccttc aattccagct tcttcgagtt gatcatatac gtctctcagg acgtctaatt
    19021 cctcgccctt tttataattc gctatttcat taattagttc tagataatcg atcttcattt
    19081 ttccacctct tagaatggtg cttttgattg tctattagct cgttctagcg cttttttctt
    19141 gagataggct tcttggtcga ttgcccattc aggaagcttc tctcgtcttc ctgtgcgctt
    19201 gtatccactg cttgcgttct taggttcact tttttctttc cttgcccaac ttcgaatagt
    19261 tgccaaatag tttttataag tcttaccaga tgattcacaa tactctgaca gtcgttcgat
    19321 tcgctcttgg taatcattag ggaattctgt tttgagtttc tccatctgct catctgacaa
    19381 aagaacattt ttatactctc cgtatttatg acggacgggc ttagccttcg attttttcga
    19441 aggcggtaac tctcttatat attcttttgt attattaaat gtattattaa tagatgtatt
    19501 attatctttg actttttcgt caataggggt attgcgtttt tcgtcaatag ggtattgatt
    19561 aattcgtagg tacctattga ttatttgatt ggtaccctct ttgtaaatga tttcccgatt
    19621 caagtatcca aacttaatca aatcacttac ccatcgcgat atggtctctt tattcacacc
    19681 atataaatct gcaaagtact cattgcctgc ccaacaaaag cctctttcat tacacaaggc
    19741 cgttatctct ccgtataaca acttagtatt tggtttaagt cttttgtcgt accttacgtt
    19801 ggctggtata atcgcataat aacttcgatg ttctgtcatt tttcccctcc aatatttaac
    19861 tttttgattg ttttctggtt taatttgatc cctttgattt gatatttatt tttgaaatta
    19921 atcacaccta ttttgtgctt ctctgtgtga tggattctgc agagtgctgc aaatgtgtac
    19981 tctgcatgat caacttcttt gcgctttcgt cttcctagcg ctttgtcaaa gtgatcgatg
    20041 tcagctcctg ttttgccaca gatgcagcag actctttttg tgatgcattt gtagaagtaa
    20101 tattcttgat tcgcaggtaa aatctcatag ccttctttga aaggaatatg atgttcaaag
    20161 atgaaatcta agatgatatt tgctaagaca ttggcatcac tcacagttgt attcgattcg
    20221 tctttcaggc ttattttgcg ccctgtgaca ccttcaaaac gaaagtagaa gaattccttc
    20281 cagaagtccg ttggcatgcc tgtatcgata aaaatatcgc ctatcagcgc atagatgaag
    20341 tttcgttgct gtacggtgaa acgtctagga tcaataaaac gaatttcaat gactcgatca
    20401 ccatcgtagc cgtcatacat cgtctttagt cgatcaatgt tcacttcctc attgatggtt
    20461 gcgcttatgt cttttccttt gaactttttc agaaccgctg aatatgaatc gattaatggt
    20521 ttaaacactc atatcacttc ttatctaatt cttttctctt agctgctatt gctcgctcca
    20581 tcaaggcaca ttgctcatag cttaactgtt caatagtttc aacgttatca gctaagagcc
    20641 ctaatttatc tgtctgctca ttaacatatt cgattaaggt tttggtcata tctttaccca
    20701 tctgctcatt gaaagcttct agaatcgtct ctagcatgtt taatttcttt gtatcgattc
    20761 taggtggtgt tggaatatct tccccttgaa atacatataa tcccagtccg tgtagagcca
    20821 atgctttcac aaagcatcgc ttcaatgagt tattgatttg cattgcattt ggtttaacaa
    20881 ctggttggtt tcgataatct aaaacaggaa ataattcggt ttccgtgtgt cctttaaccg
    20941 ttactgagac agatacataa gtcccagttt catccataag aaaaggttta tattcctcaa
    21001 caagaaagtc ttgatgagtt ccagaaacaa ccctgtagtg tttgtactca ttaatagtta
    21061 ccgttgcctg tggatcattc tttttcataa tctcccacgc gtgagcccaa gataaataat
    21121 caaaatttcc ttttttcttg agaattttat ttaacttacg actaaaaagt ttttcaaagt
    21181 tcgttgtccc tttgatttca ctcatcaaat tctgcctcca tttcagcaat gtatttctta
    21241 cctggtccgt aataagagat atcaatcaag ttatctctgt cgtactcttc tagcgcatca
    21301 atcaagccat cttcgatgac gtaaatatat tcaggtttat tcgaatgctt cgatagatgg
    21361 ataagataaa catgatccca aatagttaca tggttgccta aatcatcttg atcccaagct
    21421 agttcttcat tcgtcaaaag atttcgtctg atttttcgac cacttgtttc ctcaattttc
    21481 ggcttgcccc aatcaggatc aatcaaatat tgatctagag tggaaagttc tttttccata
    21541 tgctaaaatc tccttatgat gtgttttctt tgtgactctt tgcttgccgg cggagtcact
    21601 tttttatttg ttgccatgct ttttgcttgt caatatgttg ttggcttagg atgattggtt
    21661 tatagtattt ccaccagcaa ttagcaattg ccgtccctat tcttagcgct tcagctctat
    21721 tcattgtcat caccaaaaag tctctgttgt ctgttcagtt gatcaatttc catacggatc
    21781 gcagtttctg gcaaccacat ttcaataaat gaaacagcat catcgaatct cttacgaggt
    21841 aactcgccat atcttgggat tgaaaaggta cgtttaaatt cagaccaaaa ttttgagaat
    21901 acttttttgc tgatttcttc ataagctcgg ctttcttttc cccctagaac tcccataact
    21961 ttcatatttc ctttttgctt aatttcaaac tcttgttgtc cgctaattcg catagtatct
    22021 ttaagcatgg aaacatcttt tttaacatct ttcatttctt ctaattgata gatcatcata
    22081 tcttcaattg tttgaggaac agtattcttg cgaataacat cttccatttc gttgaatgct
    22141 tcaatgtatt tttgtttgaa gtagatagct ttctttcctg taaaccccat agccagcaag
    22201 aaaaatccat ctctactaat gaagaaaact cgtcgatttc tgccatatga atctggttca
    22261 ttaccttcta caaacatctg tccaaaattg gacacatctt tttttagtgc atcaatatct
    22321 cttaaaacat gttgatgttt tttctcgaag ctttctgcca cttgtaagct cgtagtcaca
    22381 gcttctttat ttttcaaaat tactaattct tgcattattt cttctctcct ttttggtata
    22441 atttagttaa aaactggtgg tgtttatttt gaattttttt cacttaacaa tttttcaatg
    22501 gatgtcaata ataagtttta tattgagtac gtttttaaca gggataaaaa taaaaggaat
    22561 gcgccctcaa ctagaagttg aacttaatgc ttcttacttc gctgcagata tgatttatac
    22621 aaaagtaatc atttctaact attcaacaga accagctatg ttagttaatt tagaactttc
    22681 atccgctaac ttaaaccgca gatggtcagc tactccattt aagaaattaa ttgctaaagg
    22741 aggatcagtc gatgataata gaatatactc tgaagctgta ccgctaaata ttcctcctaa
    22801 aagtgctata tcttgttacc ttgcatttga agtagggaaa attaatttta ataaactcct
    22861 taatcaccaa acaaaaatga tatttactct aaatagaacc caaatccaca aaattgttga
    22921 tatcaaaaat acaaacttcc caatagagaa attagtgaaa gaattaaact gagtaatagt
    22981 aatatacatt ctagtttctc ttttctattc atttttagtc agccccctcg gttggctttt
    23041 tcgctctgta ctcagcttca gcttcatcaa gccccataaa aatccaaacc atgtaaacaa
    23101 tcgttcctat caacgcttgc ctgcttcccc aaagtcctaa agcgtagatg attagcggtg
    23161 cgctgaacac tagcgctcta ttaaattttc ccatccgctt gcctccttaa gatttccgaa
    23221 aaatttgttt ctaaaaattc aagtgtttta cttcttaaaa atagatatgt gtctcttcct
    23281 tcaactggat aatagacaaa tccatttttg tttttctcga tatcgataat atttctatat
    23341 cttgggttct ttaaaactct agaagtaaac caatcatatt ttctgttaat ccgttctagc
    23401 acttctggca acgtcatcca tctaccagta tcatcagctt tttttaactc ctcataatcc
    23461 acttgggaga taattacata gccttctgga attgggattt ttgcttctag atattgcatt
    23521 agctgttcct ccctttacaa ttcgtacata gtgataatcg aatctataat tctgtttgct
    23581 tctgcagaag tctttttacc gtttaaaatt aaagataagt agcttttgct aattccaaat
    23641 ctttcagcaa gcatggtgta agttaagaac tttgaacttt cgacatattc tttgattttt
    23701 tctctatctc gttgagtgat ttctgcaatg tcagtcatac taaaactcct ttctaaccaa
    23761 tttcctctaa atccatttga gggtaatatc cttctttttt tagtaattcg tagataaata
    23821 gacgcccttt ctgtgtccat ttggtattca ttacaatttt agtgccacca tcggctttcg
    23881 ggatctcagt tgtatgagat tttgtgtatc cttggttcat gtgttttttg cacaataacc
    23941 attggttacc gacttttttc tgaataccta gtttatgaag taatttattc atctgttgtg
    24001 gagacatccc ataatctgct gcaatctgac taattgttac tgaatctgta gaagataata
    24061 tgctatctaa ataggagatt ttaggttcgt actctgcaat cttttgttct gcgattaatc
    24121 ttccagttct ttcttctttt agttgagttg ctaattgaat gattgtatct ggattaagca
    24181 aagcttcttc tactttttct ggagttagat aacctccatg ttttctaatt gctggcaaca
    24241 cttcacttgt tacccaacgt ttaaattttt tggcagaggg aagttttgat tttaagatta
    24301 aactgtacaa accagattcg ttgatgattg tcatctctct tgattgacct gaggtcgtga
    24361 ttcgcgaccc catcttatct tccaaatcta catgccgcga caaagcatct ttagtgtttg
    24421 aataacccaa aacactcgca acgtctttgc ctacaaaata tggttcatca tttactaaaa
    24481 ttgtccgaac ttcgttttgt tcgaaattaa aaatttgcgg tgtgttcata tttctcattc
    24541 ctttctttta tattcgtaaa caaatttaac aacttttttc taaattcaat tgacagtatc
    24601 tgagtaatac tctatactaa gagcataatt aaataagaca taaaaacatt gattttaaag
    24661 ctttcttggc ggtctgcatt tacatatcaa tagtgttttt tgttgtcttt ttagttgtta
    24721 aatctgttta caagatatag tatagctcat tactcagaat tagtcaaatt atttctgcgt
    24781 atttttctaa actttttttg taaacattta gaaaggttgt tttatcgatg aatacctatg
    24841 aaataataaa agacttggcg aagaggaaaa agatatctat acggcagtta gaaattaatt
    24901 ttggatattc taacggttat ctagcaaagt ggaaaaataa tactcctaat tcagatgaat
    24961 taagtcggat tgctgactat ttcgatgtct ctgtagacta ccttctaggt agagaagaaa
    25021 gagaaacccc taaacatgtg gatttatcag aagacgatac tgttttttct tttgatggaa
    25081 aagaaatatc taaggagaca atgcgtaaag cgattgcaat tgctaaagct ttagaggaaa
    25141 atgaatagtt ggagtgatgg tttgtatgta tttgaaattg aaagaaatgt tgagcgaata
    25201 caatttgaaa gtagtttata tggagatgaa agagcctggt ttctactatc caaaacccag
    25261 aatcattttt ttgaatgaaa atctatacgg tgaaactgca gaagcctttc atttatctca
    25321 tgaacttgca catttcagcg cctctcattt tgaattttca gttttgtacg atacttctac
    25381 aacttttcac tcaaaatttg aaacagaagc agataaaata gctattctaa ttttattaaa
    25441 tatctatatt gagaacgaac tgactgatga atctcagttt aatctcgaaa aatttatgga
    25501 gtattattct attcagaata agctcagata cacttgttat gctgtctgcc aatgctattt
    25561 taagaaaaaa tattcttacg caaggcaata tgtatgaata catcaagaat tattaaaaaa
    25621 accgatttca gcaaaacatt aaaaaagccc gtgctgcaac acgggctctt tcctcatata
    25681 tgagcttcta caaaaaaaat catatcacag aaatgaggaa gaaaaaatgg aaaaagagat
    25741 tttaataaaa tctccaggaa aaaccttgat taaagtaact gatgattcta tttcaattat
    25801 tagaaaagga tttataaatt tagttaatca aggtattaaa ggagaaaaaa ctatcccctt
    25861 taaaaatata tcagccgtac aattaaaaaa accaggtatg agtaatggtt atatacaatt
    25921 taccttgcta ggcggaaacg aaagtcgcgg aggtatatta gcggccacga aagatgaaaa
    25981 caccgttatg tttac
//
LOCUS       DAZAID010000035        24260 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000035 DAZAID010000000
VERSION     DAZAID010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.035"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACG9BE_001337"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACG9BE_001337"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFN3032089.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACG9BE_001338"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACG9BE_001338"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFN3032090.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACG9BE_001339"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACG9BE_001339"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFN3032091.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="ACG9BE_001340"
     CDS             complement(3373..4680)
                     /locus_tag="ACG9BE_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFN3032092.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="ACG9BE_001341"
     CDS             complement(4683..5264)
                     /locus_tag="ACG9BE_001341"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFN3032093.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="ACG9BE_001342"
     CDS             complement(5836..7128)
                     /locus_tag="ACG9BE_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3032094.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="ACG9BE_001343"
     CDS             complement(7125..7748)
                     /locus_tag="ACG9BE_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032095.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="ACG9BE_001344"
     CDS             complement(7751..8587)
                     /locus_tag="ACG9BE_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3032096.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="ACG9BE_001345"
     CDS             complement(8604..9491)
                     /locus_tag="ACG9BE_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3032097.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="ACG9BE_001346"
     CDS             complement(9507..10790)
                     /locus_tag="ACG9BE_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3032098.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="ACG9BE_001347"
     CDS             complement(11473..12156)
                     /locus_tag="ACG9BE_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFN3032099.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="ACG9BE_001348"
     CDS             complement(12174..12791)
                     /locus_tag="ACG9BE_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN3032100.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="ACG9BE_001349"
     CDS             complement(12801..13625)
                     /locus_tag="ACG9BE_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFN3032101.1"
                     /translation="MNKFLGFRAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="ACG9BE_001350"
     CDS             complement(13618..14178)
                     /locus_tag="ACG9BE_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032102.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="ACG9BE_001351"
     CDS             14352..15308
                     /locus_tag="ACG9BE_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3032103.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="ACG9BE_001352"
     CDS             complement(15613..16569)
                     /locus_tag="ACG9BE_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFN3032104.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="ACG9BE_001353"
     CDS             16673..17275
                     /locus_tag="ACG9BE_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN3032105.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="ACG9BE_001354"
     CDS             complement(17363..18862)
                     /locus_tag="ACG9BE_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFN3032106.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="ACG9BE_001355"
     CDS             complement(18878..19402)
                     /locus_tag="ACG9BE_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFN3032107.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="ACG9BE_001356"
     CDS             19700..20449
                     /locus_tag="ACG9BE_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN3032108.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="ACG9BE_001357"
     CDS             20440..21225
                     /locus_tag="ACG9BE_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3032109.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="ACG9BE_001358"
     CDS             21194..21910
                     /locus_tag="ACG9BE_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN3032110.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="ACG9BE_001359"
     CDS             complement(22012..23031)
                     /locus_tag="ACG9BE_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFN3032111.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24051)
                     /locus_tag="ACG9BE_001360"
     CDS             complement(23050..24051)
                     /locus_tag="ACG9BE_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFN3032112.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5221 c         4225 g         7626 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctctaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agtgattttt
//
LOCUS       DAZAID010000036        24194 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000036 DAZAID010000000
VERSION     DAZAID010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.036"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..880
                     /gene="purR"
                     /locus_tag="ACG9BE_001361"
     CDS             62..880
                     /gene="purR"
                     /locus_tag="ACG9BE_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFN3032113.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1383..2756
                     /gene="glmU"
                     /locus_tag="ACG9BE_001362"
     CDS             1383..2756
                     /gene="glmU"
                     /locus_tag="ACG9BE_001362"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFN3032114.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2850..3821
                     /locus_tag="ACG9BE_001363"
     CDS             2850..3821
                     /locus_tag="ACG9BE_001363"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFN3032115.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3827..3929
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3995..4564
                     /gene="thiT"
                     /locus_tag="ACG9BE_001364"
     CDS             3995..4564
                     /gene="thiT"
                     /locus_tag="ACG9BE_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFN3032116.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4645..5418)
                     /locus_tag="ACG9BE_001365"
     CDS             complement(4645..5418)
                     /locus_tag="ACG9BE_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032117.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5685..7385
                     /locus_tag="ACG9BE_001366"
     CDS             5685..7385
                     /locus_tag="ACG9BE_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFN3032118.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7355..7573
                     /locus_tag="ACG9BE_001367"
     CDS             7355..7573
                     /locus_tag="ACG9BE_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFN3032119.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7593..8483
                     /locus_tag="ACG9BE_001368"
     CDS             7593..8483
                     /locus_tag="ACG9BE_001368"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFN3032120.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8501..8785
                     /locus_tag="ACG9BE_001369"
     CDS             8501..8785
                     /locus_tag="ACG9BE_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032121.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            9272..9886
                     /gene="gmk"
                     /locus_tag="ACG9BE_001370"
     CDS             9272..9886
                     /gene="gmk"
                     /locus_tag="ACG9BE_001370"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFN3032122.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            9891..10196
                     /gene="rpoZ"
                     /locus_tag="ACG9BE_001371"
     CDS             9891..10196
                     /gene="rpoZ"
                     /locus_tag="ACG9BE_001371"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN3032123.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            10449..12857
                     /gene="priA"
                     /locus_tag="ACG9BE_001372"
     CDS             10449..12857
                     /gene="priA"
                     /locus_tag="ACG9BE_001372"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFN3032124.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            12872..13363
                     /gene="def"
                     /locus_tag="ACG9BE_001373"
     CDS             12872..13363
                     /gene="def"
                     /locus_tag="ACG9BE_001373"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFN3032125.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            13356..14294
                     /gene="fmt"
                     /locus_tag="ACG9BE_001374"
     CDS             13356..14294
                     /gene="fmt"
                     /locus_tag="ACG9BE_001374"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFN3032126.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            14294..15652
                     /gene="rsmB"
                     /locus_tag="ACG9BE_001375"
     CDS             14294..15652
                     /gene="rsmB"
                     /locus_tag="ACG9BE_001375"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFN3032127.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            15665..16405
                     /locus_tag="ACG9BE_001376"
     CDS             15665..16405
                     /locus_tag="ACG9BE_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFN3032128.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            16402..18471
                     /gene="pknB"
                     /locus_tag="ACG9BE_001377"
     CDS             16402..18471
                     /gene="pknB"
                     /locus_tag="ACG9BE_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFN3032129.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            18636..19535
                     /gene="rsgA"
                     /locus_tag="ACG9BE_001378"
     CDS             18636..19535
                     /gene="rsgA"
                     /locus_tag="ACG9BE_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFN3032130.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            19548..20198
                     /gene="rpe"
                     /locus_tag="ACG9BE_001379"
     CDS             19548..20198
                     /gene="rpe"
                     /locus_tag="ACG9BE_001379"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFN3032131.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            20199..20837
                     /locus_tag="ACG9BE_001380"
     CDS             20199..20837
                     /locus_tag="ACG9BE_001380"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFN3032132.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            20992..21846
                     /gene="mreC"
                     /locus_tag="ACG9BE_001381"
     CDS             20992..21846
                     /gene="mreC"
                     /locus_tag="ACG9BE_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFN3032133.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            21850..22359
                     /gene="mreD"
                     /locus_tag="ACG9BE_001382"
     CDS             21850..22359
                     /gene="mreD"
                     /locus_tag="ACG9BE_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFN3032134.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            22614..24188
                     /locus_tag="ACG9BE_001383"
     CDS             22614..24188
                     /locus_tag="ACG9BE_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFN3032135.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         8133 a         4393 c         5201 g         6467 t
ORIGIN      
        1 tttttgaaga aaaacttata taatgtaaaa cgataaaatt tcttaaaagg agttggaata
       61 ggtgaaagta cgtcgcagtg aacgtctagt tgacatgaca cagtatttat tgaatcaccc
      121 gcatgaattg atttccttaa caagctttgc cgaacgttat gaatctgcta aatcttctat
      181 cagtgaagat ttgggaatca tcaagaaaac atttaaagaa cgagggtatg gaaccttgga
      241 aacaatcccg ggtgctgcag gtggtgtatt gttcatccct gaaatttcat atgaagaggc
      301 gaaaaaatat attgaatctc tagcagaacg tctatcagaa caagaccgtc tgcttccagg
      361 aggatatgtt tatctctctg atttgttagg agaacctgaa ctgctgcgtc aagttggaag
      421 aatcattgct tctaaatacc ttggtgaaaa aatcgatgct gtaatgactg tagccacaaa
      481 aggcgtacct attgcccaag ctgtgtccta ttatctgaat gcgccattcg ttattgtacg
      541 ccgggactca aaaataacag aaggatcaac agtaagtgtc aactatgtgt ctggatcttc
      601 tgaaagaatt gaaaagatgg aactgtctaa gagaagtttg aaacgtggtt cacgtgtcct
      661 tgtagtggat gatttcatga aaggcggcgg aacagtcaac ggaatgaaga gcttgattga
      721 agaattcgaa tcagaacttg ttggcatcac tgtatttgcc gaatcaaaat tcaacggtca
      781 tcgcgccatc gatgattata cgtcactgct ttatgtcaaa gatgtagata ctcaaacgaa
      841 aaatattacg gttgtgccag gaaattattt ttcagaataa gaggttgtga aaaagccgct
      901 tagtttcgag tagtaagaaa aattttgtga aaatggcttt ccacattttt tcataaattt
      961 ggcttaatac cgagaaactg gcttttgaac accgtttatc tagaggttgt gaaatcagcg
     1021 tcctgtcctg agtattagct caataaacgg aaaaattgct cctcatattt tgccgtttat
     1081 tgagctaata ccgaaggtca gctgattgaa aaacgtttaa tgaagaggtt gtaagcctac
     1141 cgtttaactc taagtcacaa gaaacgattt caaaaaacag cttttcatgt tttatgagta
     1201 gtgtgacttg tgaccgagga gttggcagga gagcaccggt taccaaatag ataggcccgt
     1261 tatggtctat ctattttttt atccagaaaa cgctgggata aaagaaaatc acttgttttg
     1321 ttgaaaaaat gggtatactg ggataaactg aaatcagcaa actatattta ggagtgacgc
     1381 agttggacgc acgttatgca attattttgg cggcagggaa aggtacccgc atgaagtcaa
     1441 aattatataa agttttacat cccgtttctg gtaaacctat ggtagaacat atcattaatc
     1501 gggtcagtga aacgaaacca gatgaagtga ttacgatcgt tggacacgga gcagaacaag
     1561 ttaaagcaca actaggagaa cgcagtaaat atgcgttaca agcagaacaa ttgggaacag
     1621 gacatgctgt tttgcaagct gcttctttct tggaaggaaa aaaaggaaca acgttagtta
     1681 tcagtggcga tacaccatta ttgactactg aaacgctgaa caatcttttt gaatatcacc
     1741 aaggaaaaaa tgcaagtgca acgatcttga cggcacaagc agaaaaccca acaggatatg
     1801 gacgaatcat ccgtgaccat attgggatcg tagaaaaaat cgtggaacaa aaagatgcaa
     1861 caccagaaga agctcttgta caagaaatca atacaggaac ttactgtttt gacaacgaag
     1921 ctttgttcga tgcgttaagc aaagttggaa caaacaatgc tcaaggtgaa tactatctaa
     1981 cagatatcat cgaaatctta aaagaagaag gccatacagt tgctgcttat caaacggatg
     2041 attttgaaga atcaatggga gtcaatgatc ggatcgcgtt agctaaagca aatgagatca
     2101 tgagaaagcg aattaaccag atgcatatgg tcaatggcgt aagctttgtc gattcagcaa
     2161 cgacatacat cgatgcaggc gtagaaatcg gtccagacac attgatcgaa gcaggtgtac
     2221 aaatccaagg aaatacagtc attggttctg attgtgtcat cgggtcacat tcaaaaatcg
     2281 tagacagtag gattgaggat cacgtcgtca ttgaaaactc agtcattgaa agcagccatg
     2341 tcaagaaaca tgcagatgtg ggaccttatg cacatttacg accaaaagct gaaattggcg
     2401 aaaacgtcca tatcggaaac tttgtggaag tgaaaaatgc tcaaatcggc aaaggaacta
     2461 aagtcggtca cttgacttat gttggtgatg ctacgctggg agaagaaatc aatgtcggat
     2521 gtggtgttgt cttcgtcaac tatgatggaa agaacaaaca tcatacgact gtcggcgacc
     2581 acagctttat tggttctagc acgaatatca tcggacctgt cgaggtagct aaaaacagtt
     2641 cgattgctgc cgggtcaaca attacagaca atattccaga atatgcgctg gcaattgcga
     2701 gagcaagaca agtcaataaa gaaggatatg caaaaaaact cccttatcta aactaagact
     2761 tttgcaaaat cacttgattt atttaaaaaa tatgactact atttattagg tagaattcta
     2821 gagttgtgaa aaagaaagtg gaggtcccca tgtcaaaaca ttactttgac ccaagattaa
     2881 aaatttttgc gttaaattcc aatcgcccat tagcagagaa aattgcagca gcagtcggcg
     2941 tcgagttagg aaaatcatct gttactcaat ttagtgatgg agaaattcaa gtaaacattg
     3001 aagaaagtat tcgtggatcg catgtctatg ttattcaatc aacgagcagc ccagtcaatg
     3061 ataacttgat ggagttgctg atcatgattg acgcactgaa acgagcaagt gcaaaaacaa
     3121 tcaacgtagt catgccttat tatggctatg cgcgtcaaga ccgtaaagca cgtgcacggg
     3181 aaccaatcac agcgaaatta gtagcaaaca tgattcaaaa agctggtgca acaagaatgc
     3241 tgactttgga ccttcatgca gtccaaatcc aaggtttctt tgatattcca gtcgatcatt
     3301 tgatgggtgc gccacttatt gccaattatt tcttagaaaa aggaatcaaa ggcgatgatg
     3361 ttgtcgttgt ttcaccagac catggtggtg tcacacgggc acgtaaatta gcggaatttt
     3421 tgaaatctcc gattgcgatc atagacaaac gccgtccaaa agcaaatgtt gcagaagtaa
     3481 tgaatatcat tggacaagtc aaaggcaaaa cttgtgtact gatcgatgat atgatcgata
     3541 cagcaggtac gatcacattg gctgccaatg ctttgaaaga agctggtgca actagtgtgt
     3601 atgcttcatg tacacatcca gtattatccg gccctgcttt acaaagaata gaagattcag
     3661 ctatcgagcg tctagttgtt acagactcga tctatcttcc agaagatcgt aaaatcgaca
     3721 aaatcgatga agtaagtgtt ggcggactaa tgggagatgc aatcaaacgt atccatgaaa
     3781 acaaaccagt tagtccattg tttgaaacaa aaaataaata ataaataaac tcacaaaggg
     3841 gagtccaatt gggctgagat tgaatctatt tctaaaccct tcgtacctgt atcggttatg
     3901 cgagcgtagg aattgtgaat aaacagctgt ttgactattt tcgaaagctg cttcctcctt
     3961 tgtgactttt ttattcaaag gaggaagttt ttttatggga aaaaatagag tttgggtaga
     4021 aggaacgatt gttgcagcgt tagcgatggt gttgtcgttg attccgatcc agatcggcag
     4081 cagtttttct atttcattag ggcagattcc attgactctt tttgcgttaa gaagagggac
     4141 aaaggcaggg atgctggcag gtttcatttg gggcatccta cattttccgt tgggacaagt
     4201 ttattattta agtgtcgttc aagtgttgat tgagtacccg attgcctata cttttgcagg
     4261 cgcagcaggg ctcatggctt caaatgtcca aaaaggctat gtagacgata atacgaaaca
     4321 aattagaaaa agcatcgtgt ttggtgcatt gattggcgca ctttccagat atttttggca
     4381 ttttattgca ggagtggttt tctggggagc gtatgcacta tgggggatga atccttggct
     4441 cttttctttc gtaatgaatg gtgccagcgg gttgagtaca gctattgtaa cgatcatcgt
     4501 tttattaggg atccaacaaa cgactccttc gatttttgtg ccagataaac gacaggagct
     4561 gtaaataaaa aagaagcaag aaacttgaga cagaatcatg tcctagtttc ttgtttcctt
     4621 tttatttttt gataagaaat agatctactt caatggcaga taagaaagat cggcatcttc
     4681 taccaattgc cagtgtcgat cggtgacaga gtagattaaa gtagcgatga tttcctcttc
     4741 tcgataccgg gtgcctttta atttccaaat ataatcttca atcacataga ttgggatacg
     4801 atatttacca tcgatcaaac caacttgatc aaaagaaaaa atgatccctt cctgcatcaa
     4861 attacgcatt tctttcaata attccacact gattttttca acaggtgttt cttttttgaa
     4921 aaaagatttt tttacattga tcttatctga caagatcgaa cttaatttac tagcaaaaat
     4981 cctataaaat tcatctaaat agcgataata aatccgattc agctgatcat taagatcgaa
     5041 gtatacaaaa ttattctgta gtttataaaa aaacggcgaa tgaagatgtg ttttcatatg
     5101 accaaaatat aataattctg aaatttctaa aggtgtcaat tctttcagca tcgttaaatc
     5161 agtaaagtcg atccacttca attcatcgaa tccacgcctg ttacgagaag aggaaaagaa
     5221 acgctgaata ttttctgtac cacggattac tttcagccct gtatgggatt catattctcc
     5281 agcagaagca gatggatcaa gcaagagcaa gttttgagga aaatgcacga tactgtttgc
     5341 aaagtcagta gcagtcagcc cttttgttaa aacagcatta cttgtggtat caatatgtac
     5401 ataaactgac tcagtcatca tgttcctcct cttatttcct cttaatctct tttattttac
     5461 aacatttgca ttaaaaaagc ttgtttcttt tattttaaaa ttccattaca aaatcacgtt
     5521 ttcattgaaa acactctcat tttcaattaa tttttgtgat ttttgtattt ttttatttaa
     5581 aggaggtttt atgaattgtt ttcttatgga atctacaaag tcgaatacga gactcatgac
     5641 ctttttatga cgtactggta tacttgaaga aaaaggagag cgcgatggag ataaaaaaag
     5701 tttacttttc acagaaaatg tcgctgtatt cgcctatcca gcaagcaaaa caaccccttc
     5761 cattttttca aagtcatcaa gaaagtagag acgctttttg gcgttctgca aaaaagcaat
     5821 gtgcttttaa agcagtatcg aaaaaatata ccgtctattt tactattgct aaaagtagag
     5881 gagatgttat ttttaataat ctgttgattg aaggtgaaac ttttgaatgg aaaaaatttt
     5941 ttcggtatat ggaagcttgc gctcgttttt ttattaaaga aaactgttgt tttttatttc
     6001 aatctcctct atcagaagaa tggttgcgta tttttcataa aaacggctat caaggaacca
     6061 cacaattaaa taaaaagctg gtctatcata cagcacttgt tttaggtaga ggaggagcgc
     6121 acggtgctta tcagattggt gtgtggcagg cgctgaagga acatgatatc aactttgaga
     6181 ttatcacagg tacctcggta ggtgcgttga acggcgcatt gattttacaa ggagatatga
     6241 aaaaagctgt gaatttatgg aaaaagctgt cgacaagaca agtgcttgcg ttaccagaaa
     6301 tggctgcagt tgaagacctt cgtgaacgct tctatcgaga aagacgccaa atgacgaaaa
     6361 cagcactaat tgaaggcggg gtttcttctg ctccattgga aaagatggtc aaagacaatt
     6421 tggatttgtc cttactcaaa cataatccca aaatacgttt atatacggtg tctactaaac
     6481 tgccagaact agcggaagta gtgactgata tccaacagac aaaaacagaa gaaataccag
     6541 attggatact cgcctctgct tcattttatc ccgctatggc ttatcgaaag atcggaaaaa
     6601 ataaatatgc agacggtggg tatcgcaaca aaatacctat tgatattgcg attaacaaag
     6661 gagcaacaga ggcatttgtc gtagatgtgc aagggccagg cccagcgaaa aaaattcgta
     6721 tgccagacat ttttatccat tggaaatgtc aaacattgtg gacactaggc agctttctgc
     6781 tttttgatag ccaaaggaac caattaaatt tacaattagg ctatctggaa atgaaaaaac
     6841 gattaggcta ttactatggg aactggtata catttcattc tgttaaacct gcaagaacat
     6901 actggcgagg atttttgtct tacttaacaa aagaaattca gttagaatta tcttttttca
     6961 aaagcataaa attttggcaa aaactgcgta atttatataa gaacagggtc gtaccagaga
     7021 catgcggatt ggcgatgcta gaacttttag caaaaaaaca ttttttactg cccaatgaaa
     7081 tctatcaagt agacatgatg attcaaatga tctgccaaca agatataaaa agtatgccgg
     7141 atgatttatt ggcacaaatt ggtcaattaa gtactgaaga atggcgaagg tatcgaaaat
     7201 accagcaaaa aatacaaaat gagcgcacga aaactgttta ttttgattat ctgctacaag
     7261 gaaaacgcaa ggatcgtttg cagcatgaat tgcttaagca accagttgac acattactga
     7321 tcctatattt atattatctc aaggaggaac aaccatggca caagaatttt catatgaaat
     7381 cgtagaagaa atcgctatat tgtcagaaaa caacaagggg tggcgtaaag aactgaacct
     7441 agtcagctgg aatggtcgcc cgccaaaatt cgatcttcga gattgggcgc cagaccatga
     7501 aaaaatgggg aaaggcctga cattgacgaa cgaagagttt gaacaactgc aaaaagcaat
     7561 agaaaatatg taatgaggaa ggaccctgtc gaatgaaaag tatgactggt tttggcaaag
     7621 ccatccgcga aacaaaagcg tatcagttag atattgaaat aaaaagtgtc aaccagcgtt
     7681 ttctagatgt tcaaatacga agtcctaaaa tcctgaactt tgttgaaaac gattttcgtc
     7741 agcttattaa acagcagtta agccgcggtc gaatcgaagt atttgtgaat ctagcttata
     7801 ttggcaaaaa cgaaaaacag atcgtcatca actgggaatt gattgattca cttatcactc
     7861 aattgacaga cggaattcaa caacgatacg gtagtcaaaa tcaagtgtca attagtcgga
     7921 tggcggaacg cttcgtgctg gacgaaaact tcgtaacggt agaagaaaaa ccggaagaga
     7981 gcatggaaga attggcacag ttagctctag atacattgaa aagcgcactt gattctattg
     8041 aggcgagtcg agaaaaagaa ggtcaagctt tagctttagt gttgaaaaag aatactgaag
     8101 aattcaaaga agtactaaaa aaactcgata gttttgtaga aatctatgaa aaggaatttc
     8161 aaataaggta tcaaaaaaag ctggaggaat ttcttggagc gaaagtcgat gagcaacggt
     8221 tgttgacgga gctagccatc ttgttggaac gcggagatat tcacgaggaa ctggatcgtc
     8281 tggcgatcca tgtacaaaaa ctagatgaac ttatcgaagc acaatgtcct gtaggtcggg
     8341 aattagattt cctgatccaa gaaatgaatc gggaaatcaa cacgatcggt tccaaatcaa
     8401 atgcaatcga aattaaaaat caagtcgtcc agtctaagac gatcttagaa aaaatcagag
     8461 aacaagtaca aaatattgaa tgacgaaaga ggtagagata atgaaatatt atgacgtaac
     8521 gttccacgaa ttaagtggaa aaactgtggt taaacgggat atcccttctg aaaaaaacgg
     8581 ctttgacgta tggaaagatg cctgtgcaga ctataatgag aacgaactgt ttattttgat
     8641 caacgacggt gcttacgtga cgatgaatcg gaaatttatt gttcgaattg acacagaaga
     8701 ggtagaagat ccgacagaaa aagcacgttc tcgaaaagat gagatcatgg gtgtagtgaa
     8761 tactttgtcc aatatgggct tttagcaagg atgctgaaca aagaaataca ataaataaac
     8821 agaattggat ttcttcgctt agcttctttg ctctaggaat acatttaaag gttgtgacaa
     8881 aattcgtgtc acaatctttt tttattttga ataagtgatg ttcaaaaacc attttctcgg
     8941 tattaagcca aatttatgca aaaatgtgag aaactatttt cacaaaattt ttcttcatac
     9001 tcgaaactaa tcggcttctt cacgacctct tatacacggc gttcaaaagc ttgcatctgc
     9061 gataataacc ccaaccaaac gaaaaacatg agaagctgtt tatgtttggc tgttcttatt
     9121 acttgatgca ggacagcttt tttacgacct cttggtacag ggtagctttt ttaccaattt
     9181 ctagctataa ttaaagataa ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa
     9241 gtacaaaaag actagttgaa aggacgtcgt catgtcagag cgtggattat taatcgtgtt
     9301 gtctggtcca tccggtgtag gaaaaggcac agtacgaaaa gcaatttttg agagtgagga
     9361 taatgatttt cagtattcga tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga
     9421 aggcgttgat tatttcttcc gaagcaaaga agagtttgaa tcaatgatcg aagctggaga
     9481 gatgctggaa tacgcagaat atgtcgggaa ttattatgga actcctttgt catatgtcaa
     9541 caagacattg gatgaaggaa aagatgtttt cttggagatt gaagtccaag gagcaaaaca
     9601 agtcaaagaa aaagtgcccg atggtgtatt catctttttg actccgccag atttagcgga
     9661 attaaaatca cggatcgtag gccgaggaac agatgcagat gaagtaatcg aaaaacgaat
     9721 gaaagttgca aaagaagaaa tcgaaatgat ggctctttac gactatgctg ttgtaaatga
     9781 tgaagtgccg ctagcagttg aacgaatcaa aaacattatt gccagtgaac attttcgagt
     9841 agatcgcgta atcggtaaat atattaaaat gttaaaggag atgtagtctt atgatgttaa
     9901 aaccttcaat tgactcacta ttagatcgtg taaattcaaa atattctcta gtgattttag
     9961 caagtaaacg cgcgcatgaa ttggatgcaa gtgcacagcc tactctagat tcttttgatt
    10021 cagtaaaaag cgtcggtcaa gctttagaag aaatcgatgc aggaaatgta gtcaatgatc
    10081 cacatcctga attaaaacgt gaacgtttga agatggaaga agaggaacgt caagcacaaa
    10141 aagagcgtga acaacacgaa ttggaatcac gtatccgtga agagcaaaaa atgtaataaa
    10201 gaaaaataga gcgaacatct aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt
    10261 cgccacttat tgtctggtag aaaaggctgt gtctgaactt tttgacacag ttcttttttt
    10321 tcttatctgc tgttaatttt ttgttaggat ttggcagaag ataagacaaa tccatcgatc
    10381 tctagtaaaa taggcaagga actttttgtc gcttgataag aaaagacaaa aaaaggagga
    10441 aacgaaccat gacattaaca gctgaagtca ttgtggacgt acctactatg cagaccgatc
    10501 agccattcac ttatttggtt ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag
    10561 tagaagtccc ttttggtaat ggcaatcgtc atgtccaagg atttgttatg gctatccaaa
    10621 aatcagaaga atcagctaat ccatctttga aggcagttat tcgtttgcta gaccttgctc
    10681 cggtggtcaa tgaagaatta ctttcattag ctgattatat gaaaaaaatt acttacgcat
    10741 tcaaaatcac ttgtctacaa acgatgcttc cgagtgtgat gaaagcagaa tatgataaat
    10801 tgatctatcc tttggcagac acaccagaag ttcaggtatt gtttgatcag cgagaagtga
    10861 tttcctggaa ggaagcagaa gaagaaggca gcctttctca attgattcgc tggcgccaag
    10921 agcaattagt agatatcaaa tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt
    10981 tggtccgttc tttgttaact gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa
    11041 atgccaagaa acaaaaagaa cttcttttgt gtttatctga actgagccaa gaagaaccaa
    11101 ttgcttattt caaaaataaa ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg
    11161 gctggctgga gtttatagaa tcagaacgtt atcgcgaccc ttataaagac cgagtatttg
    11221 accaaacgac ggcattggaa ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca
    11281 cagcaggtca gcagcaaaaa gatcaggtct ttttgctgga gggaatcact ggcagcggga
    11341 aaacagaagt atatcttcag gcaatcgctg atgttttgtc agaaaataaa acggctatta
    11401 tgttagtgcc tgaaatcgca ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg
    11461 gagaatcagt tgcggtctta catagcggac tttcccaagg tgaaaaatat gatgaatggc
    11521 gcaagattga gcgggaagaa gcgcaagtag tagtaggcgc acgatctgct atttttgccc
    11581 cgctgaaaaa tatcgggctg atcattatcg atgaagaaca tgaatcaagt tacaaacaag
    11641 atgagacacc gcgttatcat gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt
    11701 gcccaatagt tctaggaagc gcgacgccct ctttagaatc aagagcacga gctcaaaaag
    11761 gcgtttatca gttattgcag ttaaaccacc gagccaaagc agctgcacag cttccagcta
    11821 ttgagatcgt agatatgcgt gaagagtttc agaatcatcg gacctctact ttttctgcta
    11881 atttgcagga aaaaatacaa aatcgattag acaaaaaaga acaaactgtc ctactgctca
    11941 atcgtcgagg atattcgtct tttgtgatgt gtagggactg tggatttgtt cttccatgtc
    12001 ctaattgcga tatctctctt acactgcata tggatacacg ttcaatgcgc tgccattatt
    12061 gcggacatga agaacctatt cccaatcgct gtcctaattg tggcggcaat aaaatccgct
    12121 actatggaac aggtactcaa aaagtagaag aggaactccg agaactttat ccgcaagcta
    12181 ggattttgcg tatggatgta gataccactc gaagaaaagg ggcacacgaa cagattttac
    12241 aaaaattcgg tgcaaaagaa gcagatatat tattagggac acaaatgatt gctaaaggtt
    12301 tggactttcc agaagtgaca ttagttgggg tgttgaatgc tgatacctcc ctgaatttac
    12361 cggattttcg ttctagtgag catacattcc aattgcttac ccaagtttca ggacgagcag
    12421 ggcgagcgga aaaagcaggc gaagtcgtga tccaaacatt caatcctcaa cattacgcca
    12481 tagaattggc taaaaaacaa aattatgagc agttttatca gcaggaaatg catgtacggc
    12541 atcgtggagg ctatcctcct tattacttta ctgtgaaaat aaccgccagc catcctgaag
    12601 aacaagtagc ggcaaagaaa atctttcaga ttgccaatca gctaaaagaa gtgttaagcc
    12661 ctcaaagctt gctattaggg ccaacgccca gcatgatttt gagagtgaaa aaccgatatt
    12721 attatcaact aatcatcaaa tataaacatg aaccgaatct tcctcatgtt ttggatgaaa
    12781 ttttgaatgg aagccagaaa gaacagcgac aaggtctttt tgtggcaatt gacaacgaac
    12841 cattaaactt tatttaagaa agggccaaat tatgcgctat cctatattga tccaccctaa
    12901 tgataaatta aaaagaactg ctcagccaat cgatgtaatc acggacgaga caatcgcttt
    12961 attagacaat ttatatgaga caatgattgc gaatgacgga atagggatcg ctgctcctca
    13021 agtaggacaa aacaagagaa ttgctgtgat cgaagtagat gaaggcgaga aatttgaact
    13081 gattaaccca gaaatcatcg aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag
    13141 tattccgcat gtctatggaa cggtcaagcg agcagatgaa gtgaccgtgc gttattatga
    13201 tcgagacgga gaagagatag aagtgacggc ttttggctat ttagcccgtg ccttccagca
    13261 tgaaatcgat cacttagacg gtatcttgtt cattgagaaa atgatacagc agatcccgga
    13321 agaagaactt gaagaatata tggaggaaca tcttgatgac taagctagta tttatgggca
    13381 cacctgcttt ttctgtgccg attcttgaag ggctgataga atcaggttac gagatccagg
    13441 cagtggtaac acagcccgac cgtccagttg ggcggaaacg agtaattacg ccgacaccgg
    13501 taaaaaaagc ggctctgaag cacggtatcc gtgtgctgca gccagagaaa atctccggtt
    13561 cgccagaaat ggaagaaatc attgaattgg tgcctgatgt gatcgtcact gcggcatttg
    13621 gccaattctt gcctgaaaag ttattgcaag taccaaaatt aggggcaatc aatgtccatg
    13681 cttctttact tcctaaatat cggggaggtg cgcctgttca ttatgccatc atgaatgggg
    13741 agaaagaaac tggtgtaacg atcatggaaa tgatcaaaaa aatggatgca ggcggaatct
    13801 atgcgcaaga aagcatacca atcactaaac aagatgatgt agggacgatg tttgaaaagc
    13861 ttagcctttt aggaagaaag ctattgctag aaacattgcc caatattttg gatggacaaa
    13921 aaccagtacc tcaagacgag tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag
    13981 aagcaatcga ttggcataaa actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc
    14041 cttggccaat tgcattcacg acctatgagg gcatacgctg gaaattatta tcggttgaag
    14101 tattggcaga aactacagac aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc
    14161 tatggattgc ttgcggtaaa cagaccgtct tagccatcaa tgaattacag ccagctggta
    14221 aaggaaaaca ggccgtacat gatttcctta acggaagcgg tcagcaagtt cagattggtc
    14281 agcaggtgaa ataatgaaaa aaatccctaa aaaaatgaaa aaatcagtac gatatatggc
    14341 attactgaca ttagagcgag tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga
    14401 gatgatcaaa caaagcgagc taaatcaaaa agatgtgggt ttatttacag aattgatcta
    14461 tggtacgatt agccatcagc gactattgga attttacgtt gcaccattga tcgctaaagc
    14521 aaaaaaagtg gatgactggg tcaagatgct tctttatctt tctgtatacc aattagaatt
    14581 tttagataaa gtccctgcac atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa
    14641 aggaaatcct gggacaggga aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg
    14701 agcaccagat ctgcaaggca ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct
    14761 gcctctatgg atgacgaaac gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct
    14821 cggtttatcg ctatatcagc ctagccatgc tagtgcccga gtagatacac gccgactttc
    14881 cagagatcag gccatcactg ttttgcaaga agaagagatc acagctgaaa aaagccaagt
    14941 cagtccatac ggtatagtag cgaaaaaagg gcatttagct tctagctcat tgttccacga
    15001 tggcgtcatg acgatccaag atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga
    15061 gcctgaacat catgtcttag atgcctgtgc agcacccgga ggtaagacga cgcatattgc
    15121 ttctttttta gatccgtcat gcggtggaga agtggtcgct ttagatgtac acgaacacaa
    15181 agtcaaactc attgaagata atgcagcacg tctaggtgtc tcttctgctg tctacgctca
    15241 aaagatggat gctagagaag tcagtaaaca gttcgaagca gagcagtttg atcggatttt
    15301 agtagacgcg ccttgttcag gtcttgggct gcttcggcga aaaccagata ttcgttataa
    15361 aaaacagccg gatgaattag cgaatttacc aaaaatccaa ttggaaattt tagaaagctg
    15421 cgcaccaact ttaaagtctt ccggtattct gacttatagc acttgtacga tcttaaaaga
    15481 agaaaaccaa gacgttgttt ctgctttctt ggaaagacac cctgattttg agaggattga
    15541 tgttttgacc aatcctatgc tacaatcatc aatcgaagag aaaatgttga ctttgtatcc
    15601 gcatcagttt tacacagatg gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga
    15661 aaaaatgcag attgaatatc aatcagatgt aggaagacga cgtaacacaa atcaagacta
    15721 tgccagtgta tttaccagcc gttcaggtct caagcttgct gtattagcag acgggatggg
    15781 tggtcatcgt gccggagaca ttgccagtca gatggcggta acgaatctag gcgaagcttg
    15841 ggaaaaacaa gaagctgttg acgatgaaaa aatcgcccaa tggttcattc aatcgattca
    15901 agaagaaaat atgctgattt atcagcgtgg acaagaacag ccggaatata acggtatggg
    15961 aacaacaatc gtagcggccg ctctgtcaga tgactggttc acgattgcca atgttgggga
    16021 tagcagagct tatttgattc gtgaagataa aatcatgcaa ttgacggagg atcattcttt
    16081 agtcaatgaa ctagtcaaat caggagaaat cagcgaagaa atggcagtga accatccaag
    16141 gaaaaatatt ttaacacgtt ctgtcggcat gcctggaaca gtcgaagtag atgtttctac
    16201 tcatctttgg caactaaaag atcgactgct gttatgttca gatggcttga cgaacatgct
    16261 gtctgaagaa gaaataggaa cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac
    16321 tgagctgatt gatcgtgcca atgaagcagg cggagcggat aatatcaccg ttcttcttat
    16381 cgaatataag gaggacagtg tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa
    16441 atcactggaa atatcggtag cggtggtatg gcaaatgtat ttttggcgca cgatttaatc
    16501 ttggatcgtg atgtagcagt taaagtctta cgatttgact ttcaaaatga ccagactgcg
    16561 attcgacgat ttcagagaga agctttagca gcaacagaac tcgttcatcc aaacattgtt
    16621 agtgtttacg atgttggtga agaagacaac atgcagtatc tggtgatgga atacgtcaaa
    16681 ggaatggact tgaaacgata tatccaaaca cattatcctg ttccttatga aacagcagta
    16741 aatattatgc agcagatcct ttctgctatt tctttagctc ataaccatca aatcatccac
    16801 cgagatttga aaccccaaaa cgtactgatc gataatgaag gtgttgtaaa aatcacggat
    16861 ttcgggattg ctatcgcttt atctgaaaca tcgatcacgc agacgaatac gatgctagga
    16921 tctgttcatt atctgtcccc tgagcaggcc agaggaagta tggcaacgaa gcagtcagat
    16981 atttatgctt tagggatcat cctttatgag atgttgacgg gtagcgtacc gtttgatggt
    17041 gaatctgctg taacaattgc tttaaaacat tttcaagatg atcttccatc tataaaagca
    17101 cttgatccaa atgtgccaca agcactggaa aacgtgattt tacgggcaac ggctaaagaa
    17161 cctgcagacc gttataagtc agccgaggaa atgtctgatg atctttctac agtattatca
    17221 ccagctcgtg caaacgaaga aaaatggcag cctcatgtga tggataatga aacaaaagtt
    17281 atcacacccc ttacggaaga tgcaccaatg cctgattcat tcaaatctat gcctctgcca
    17341 aaagacaagc cagaaaacga aaccttacca gaggaggaag aagttctgcc tgatcctaaa
    17401 aagaaaggac agaaaaaatg gtggctgatc ctattagcta ttcttgctgt tttaggaatc
    17461 ggaacagctg tattctttgc ttctggcggg cgaggagaag ttgttgttcc tgatgtttct
    17521 gatctttctg aagcttctgc tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca
    17581 aaaacagaag aaattgcaga tgacaaaatc gaagaaggta atgtagtgaa gacagacccg
    17641 gcagcgggaa cgacagtcaa gcaaaaacgc gaagtcactc tgtatattag tagcggcacg
    17701 aaaaaaatca aattagatga ttacagtggt aaaagttatg aagatgccga agcaagtttg
    17761 aaagaattag ggttttctgc tgacttgatt caaaaagaag aggaattcag ttcagaggta
    17821 gaaagcggaa tgatcatttc gcaatctcca tcagaagata cagaagtcga tcctaaaaca
    17881 gacacgatta ctttcacagt cagcaaagga ccagaaacag tgactttgag tagtcatgta
    17941 ggagaaaact acgaaactgt tcgtaattgg cttgttacac aaggattcaa tatcaataat
    18001 attagtgcta cttatgacta tagcgacact gtaccatcag gttcgatcat cagtcaggat
    18061 ccagcctcgg gtgaagttgt agcagaagaa acttacatca attttgtcgt aagccaagga
    18121 acagagccta aattgaatga tatctccggc tatacaaaaa gcgaagcaca aagttattta
    18181 gctagtgtcg gtgcagaata tatcgggcat gagacttccg aatattcaaa cacggtggat
    18241 aaagataaag tgattcgtac agtaccaggg gcaggaacga caattaccaa aggaatggtt
    18301 gtgaacgtaa tttactctaa aggaccagat ccagcgtctt ctagttctga aaaagactct
    18361 tcagacagtt ctagcagctc aagtagctcc agtagcacaa gcaactcttc aaaagaagaa
    18421 tcttccagct ccacaagttc atcagctagc acctcagaaa gttcaaaata agtgctgaat
    18481 aaaatatatc agaagggaat gcgacattga tttgttgcat tctctttttt gtgtttctat
    18541 ctcttttgat caattttcaa gccatatact ttgagaagtt tgcatggaat aacacctact
    18601 ttcctcttcg tatggtaaaa taaacaaaaa cggaggtggc acttatcaaa ggacaaatca
    18661 gaaaagcaat cagcggtttt tactatgtat acgcaaacgg agagacctat caaacaagag
    18721 gaagaggaaa tttccggaat cggaaaatca cgccgcttgt cggagatcag gtgatctttg
    18781 agagtgaaaa tcaaacagac ggctatcttc tggaaatctt gccgagaaaa aatcaattag
    18841 ttcgcccacc agtcacaaat gttgatcttg gaatcattgt cacaagtatc gtagagcctg
    18901 cattttctta taatctactt gaccgctttc tagttacctt ggaatacgag tcgatcgaac
    18961 cgattatttt tttgacaaag accgacttgt cagacgaccc agaaaaaatc gaagagatca
    19021 agcaaaccta tactaaaata ggctatccgg tgattgtacc caatcatacg ggtgatacga
    19081 cagaactgat ccgttacttc ccagaacggc tatcggtctt tatgggtcag tctggagcag
    19141 gaaaatcgac gctactgaat caaatatcac cggaactgaa tttagcaaca ggagagattt
    19201 ctgatgcact tggaagagga cggcatacaa cacgtcatgt ggaactgatt cctatctatg
    19261 gcgggttagt agcagataca ccaggattta gctcgattga ctttttgaca attgaagcag
    19321 gagaactgcc gagacaattt cctgaatttg tagaagcttc tgtccattgc aaatttagag
    19381 agtgcatgca tgctaaagaa cctggctgcg aagtaaaaca tagagtagaa acaggtggta
    19441 tcgcacagac acgttacgac aattatttgc aattcttaca agaaatcgaa aaaagaaaac
    19501 caatgtatgc aaaaaaagaa ggtaaacgaa aataggagga aacaagaatg aagatagcac
    19561 catccatttt gagtgcagat tttgccaatt tacaaagaga cattgagtta gtggaaaaag
    19621 gaggcgcaga ttacatccac gtagatgtaa tggatggccg atttgtccca aatatcacat
    19681 tagggccaaa tattgttcag gcaatccgtc ctgttactaa attaccgtta gacgtccatc
    19741 tgatgatcgt tgatccagaa aactacattc cagcatttgc aaaagctgga gcagatatca
    19801 ttactgtcca cgtcgaagca actcctcata ttcatcgggc acttcaaatg acaaaaagct
    19861 taggagttaa agctggagta gttatcaatc caggaacacc agtctccatg atcaaacatg
    19921 ttttgccttt ggctgatcaa gtactagtca tgactgtcaa tcctgggttc ggcggacaaa
    19981 gctttattga agaaacagta gaaaaaatta cggaactcgc tgatctacgt agagagaaca
    20041 attggactta tgcaatcgaa gtagatggcg gaatcgttcc agaaacagca aaaatctgta
    20101 aagatgcagg ggcggatgtc ttcgttgcag gatcatatat ctatggagcc gaaaatccag
    20161 tcggacaaat cgaacgatta aaagaggcat tagactaaat gaaaatttta ttagctgctg
    20221 gaggtaatcc gcaaaactgg ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga
    20281 tagaccgtgg tgcgctgtac ctgcttcaag caggctaccc agtagatctg gcagtgggtg
    20341 attttgattc attgtcgaac gaagagcaga aggaagtatt tgcaaaagcc aaagagtata
    20401 cccgttcccc tgcagaaaaa gacgatacag atacacagtt aggtatctta aaaacatttg
    20461 aacgttaccc gcaagcggaa gtcactttga ttggagctac gggcggaaga ttggatcatc
    20521 ttttagccaa tctctggtta ggtatggaac ctcgcttcaa accctttgcc catcagctta
    20581 ttatcagaga ttgtcaaaat tatttgtctt attatttgcc tggagagcat atagtaagac
    20641 agataccaga tatgaagtat ttagcttatt gctgcttgac tcccgtctcg aatcttacat
    20701 taaaaggcag caagtatgta ttggaaaata aagaagtaga atatccgaca tcttatgcaa
    20761 gtaacgaatt tctttcagat accgcagaat tttctttttc agatggtatg attgcagtta
    20821 ttcaaagcaa agattgatcc ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga
    20881 cagaatctat tatttgctaa aaaaaagctt aagaggagca acttaccttt tgtttcacgg
    20941 catttagtga taaaattgaa cacaagagtc taattaattc gaggtgtctt tgtgaagaag
    21001 ttcaatccta ataaaaatat tattattacc ttggttattg ttatcatcgt ggtgacgata
    21061 ctaagtgtga ccgctgcgca gcgagcgaat gaaggaaagc ccaatttctt ccaatccatc
    21121 gttaatgaca gtgttgcaat ggttgataaa acaatctctg ccccagtacg gtgggtacga
    21181 aatggagtag attctgtcca taatcttttt tcaacttatg ccgaaaatga gcggctaaaa
    21241 gaaaaaatcg atagctatga tgacgtttca cagcaaaaca agaattacga gagagaaatc
    21301 gaagcgttga aaaaagaact tgagttgaat cagacgctga ctagttacga aaaagtaacg
    21361 gcaaatgtca ttactcgttc gcctgacagt tggcaggaca tgctgatcgt agacaagggt
    21421 tctaaagacg gtatcgaagc aaatatggca gtcatgtcgc aaaaaggatt gattggccga
    21481 gtgatcgagg tcaatacggc ttcgtctaaa atcgaattac tgtcatcctc taatgaaagc
    21541 tccaatcatt ttccagtacg ggtatcttcg gctaatggcg aagcgtttgg tttgcttaaa
    21601 aactatgatg aaaagctcca tgccttagtg gtgacccaat taactggtga tacggatatc
    21661 aaagaagggg atgttgtcca gacatccggt cttggaggga attctccagc taacttgccg
    21721 atcggtacgg ttattaaaac gaaaccagat agttatgggc tggatcggga agtttatgtg
    21781 aaaccttatg cagaaatgta tgacgtgtca gttgtgacga ttgtccaacg gctagcggag
    21841 gattagtcca tgttgaagaa agaaacaatg aagtactatc tgccaatcgt tttgttcttt
    21901 ttgatgttga tagatggtca tttaacaaga atgctagggg agtggtcgaa aggcacctat
    21961 atgtcaaatg cccactttct gatattggca ttattatgtt gcagtatggc gtttgaaaaa
    22021 cgttatttac tgattaccac gattgttctc ggggctatct atgacgctta ctatattggc
    22081 gttatcggta tctatgcagt agctctccct ttaattgtat ggttgatgta tgtaatgaaa
    22141 gacgttatcc atgtcaacat ctttactgaa tttttcagta tgatcatctt tgtcacgggt
    22201 tatgaattgt ttacgatggt ggtccagttg atttttaaat tagcagtagt aaataacacg
    22261 tattttatta caaggttttt aggacctaca ctgctgttga acatgattat atttgtatta
    22321 ttcatttttc cctttaagaa attattcagt gatgaataat ttcttttttt atttttaggt
    22381 cgaaagcgaa aaagaatcgt tgttatctta agaaatgcta gacttaacgc ggctttttca
    22441 tgatcatttt ctgaaacgaa cagttttgtc atttttaaaa gttgttttga aacatttttg
    22501 taacataact attatatcgt tgacacactg ctatggtaca atttttatat cgttaagaaa
    22561 gacttaaata tatttagata tttattaaat taagccagtc ggaggaatga agagtgaaaa
    22621 agagtttaat atcagcagta atggtatgtt caatgacctt gactgccgta gcatcaccga
    22681 tcgctgccgc ggcggacgat tttgattctc agatacaaca gcaagatcaa aagattgcag
    22741 acttgaaaaa tcaacaagca gatgcacaat cacaaatcga tgccttagaa agccaagttt
    22801 ctgaaatcaa cacacaagca caagacctat tagcaaaaca agatacattg cgtcaagaat
    22861 ctgcacaatt agtaaaagac attgcagatt tacaagaacg tattgaaaaa cgtgaagata
    22921 caatccaaaa acaagcacgc gaagcacaag taagcaacac aagctcaaac tacatcgatg
    22981 ctgtattgaa tgctgattca ttagcagatg ctattggacg cgttcaagca atgacaacga
    23041 tggtaaaagc aaacaacgac ttgatggaac aacaaaaaca agataaaaaa gctgtagaag
    23101 acaaaaaagc tgaaaacgat gctaaactaa aagaattagc tgaaaaccaa gctgctttgg
    23161 aatctcaaaa aggcgatctt ttatctaaac aagctgattt gaacgtactg aaaacttcat
    23221 tagctgctga acaagcaact gctgaagaca agaaagctga cttgaaccgt caaaaagcag
    23281 aagctgaagc agagcaagct cgtattcgtg aacaacaacg tctagcagaa caagctcgtc
    23341 aacaagctgc acaagaaaaa gctgaaaaag aagcacgcga acaagcagag gctgaagcac
    23401 aagctactca agcttcatct actgcacaaa gctcagcaac agaagaatca tcagctacac
    23461 aatcttcaat gactgaagaa tcatcatcag ctacacaatc ttcagcaaca gaagaatcaa
    23521 caacacctga atcttcaaca gaagaatcaa cagcgcctga atcttcagca acagaagaat
    23581 caacaacagc gcctgaatct tcagcaacag aagaatcaac aacagtgcct gaatcttcag
    23641 caacagaaga atcaacaaca gtgcctgaat cttcaacaac agaagaatca acaacacctg
    23701 cgcctacaac accatcaaca gatcaaagtg ttgatacagg aaacggcaca ggaagtagta
    23761 ctccggctcc aacgccaaca ccaacacctg aacaaccaaa acctgtaaca cctgctccag
    23821 ctccttctgg aagtgtgaat ggtgcagcaa tcgtagcaga agcttacaaa tatattggta
    23881 ctccatatgt ttggggcggt aaagatccaa gtggctttga ctgctcaggg ttcacacgct
    23941 atgtttacat gcaagtaact ggtcgtgaca tcggtggttg gacagttcct caagaatcag
    24001 ctggaacaaa aatttcagtt tctcaagcaa aagctggaga cttgttattc tgggggtcac
    24061 aaggcggaac ttaccacgta gcgattgctt taggcggagg acaatatatc catgctcctc
    24121 aaccaggcga aagcgtaaaa gttggttcag ttcaatggtt tgcccctgac tttgctgtca
    24181 gcatgtaaga atga
//
LOCUS       DAZAID010000037        24059 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000037 DAZAID010000000
VERSION     DAZAID010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.037"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..333
                     /locus_tag="ACG9BE_001384"
     CDS             16..333
                     /locus_tag="ACG9BE_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFN3032136.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(424..789)
                     /locus_tag="ACG9BE_001385"
     CDS             complement(424..789)
                     /locus_tag="ACG9BE_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032137.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            997..1479
                     /locus_tag="ACG9BE_001386"
     CDS             997..1479
                     /locus_tag="ACG9BE_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032138.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1676..2566
                     /locus_tag="ACG9BE_001387"
     CDS             1676..2566
                     /locus_tag="ACG9BE_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFN3032139.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2892..5366
                     /locus_tag="ACG9BE_001388"
     CDS             2892..5366
                     /locus_tag="ACG9BE_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN3032140.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5469..7205)
                     /locus_tag="ACG9BE_001389"
     CDS             complement(5469..7205)
                     /locus_tag="ACG9BE_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3032141.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7202..7906)
                     /locus_tag="ACG9BE_001390"
     CDS             complement(7202..7906)
                     /locus_tag="ACG9BE_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3032142.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8037..8540
                     /locus_tag="ACG9BE_001391"
     CDS             8037..8540
                     /locus_tag="ACG9BE_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFN3032143.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8500..8838
                     /locus_tag="ACG9BE_001392"
     CDS             8500..8838
                     /locus_tag="ACG9BE_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFN3032144.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8859..10277
                     /gene="ffh"
                     /locus_tag="ACG9BE_001393"
     CDS             8859..10277
                     /gene="ffh"
                     /locus_tag="ACG9BE_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFN3032145.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10389..10967
                     /locus_tag="ACG9BE_001394"
     CDS             10389..10967
                     /locus_tag="ACG9BE_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFN3032146.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10971..11492
                     /locus_tag="ACG9BE_001395"
     CDS             10971..11492
                     /locus_tag="ACG9BE_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFN3032147.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11571..12125)
                     /locus_tag="ACG9BE_001396"
     CDS             complement(11571..12125)
                     /locus_tag="ACG9BE_001396"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFN3032148.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12378..12653
                     /gene="rpsP"
                     /locus_tag="ACG9BE_001397"
     CDS             12378..12653
                     /gene="rpsP"
                     /locus_tag="ACG9BE_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFN3032149.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12665..12916
                     /locus_tag="ACG9BE_001398"
     CDS             12665..12916
                     /locus_tag="ACG9BE_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFN3032150.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13040..13831
                     /locus_tag="ACG9BE_001399"
     CDS             13040..13831
                     /locus_tag="ACG9BE_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032151.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14001..14522
                     /gene="rimM"
                     /locus_tag="ACG9BE_001400"
     CDS             14001..14522
                     /gene="rimM"
                     /locus_tag="ACG9BE_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFN3032152.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14512..15273
                     /gene="trmD"
                     /locus_tag="ACG9BE_001401"
     CDS             14512..15273
                     /gene="trmD"
                     /locus_tag="ACG9BE_001401"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFN3032153.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15409..15756
                     /gene="rplS"
                     /locus_tag="ACG9BE_001402"
     CDS             15409..15756
                     /gene="rplS"
                     /locus_tag="ACG9BE_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFN3032154.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16066..16308
                     /locus_tag="ACG9BE_001403"
     CDS             16066..16308
                     /locus_tag="ACG9BE_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFN3032155.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(16396..16542)
                     /locus_tag="ACG9BE_001404"
                     /pseudo
     CDS             complement(16396..16542)
                     /locus_tag="ACG9BE_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            16599..16913
                     /locus_tag="ACG9BE_001405"
                     /pseudo
     CDS             16599..16913
                     /locus_tag="ACG9BE_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            16942..18201
                     /locus_tag="ACG9BE_001406"
     CDS             16942..18201
                     /locus_tag="ACG9BE_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3032156.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18254..19150
                     /locus_tag="ACG9BE_001407"
     CDS             18254..19150
                     /locus_tag="ACG9BE_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3032157.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            19161..20030
                     /locus_tag="ACG9BE_001408"
     CDS             19161..20030
                     /locus_tag="ACG9BE_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3032158.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIELFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            20040..20715
                     /locus_tag="ACG9BE_001409"
                     /pseudo
     CDS             20040..20715
                     /locus_tag="ACG9BE_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            20921..22585
                     /gene="alsS"
                     /locus_tag="ACG9BE_001410"
     CDS             20921..22585
                     /gene="alsS"
                     /locus_tag="ACG9BE_001410"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFN3032159.1"
                     /translation="MAEKMKQGSKSVVERLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            22598..23308
                     /gene="budA"
                     /locus_tag="ACG9BE_001411"
     CDS             22598..23308
                     /gene="budA"
                     /locus_tag="ACG9BE_001411"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFN3032160.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            23438..23935
                     /locus_tag="ACG9BE_001412"
     CDS             23438..23935
                     /locus_tag="ACG9BE_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFN3032161.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         7959 a         4113 c         4986 g         7001 t
ORIGIN      
        1 ttaggaggta atattttgct tttttatagt aattttatct taatagtagc gatattatta
       61 cttctgaata tatggatttt tgataggagt agaaatgcaa gtataggttt tagaactaaa
      121 cgaagtctgt cttctaaaaa aaattgggta tactcacaaa ctatttttta tggaggaatt
      181 gttttaatta gtttactttc ttcaacactt tattctttga atattataga tgtttccacc
      241 tcaaattcaa tatctattat tggaatcatt attgctgcaa ttattacaca actgttttta
      301 gtgtttggag agaagaaaag aagtaaaaaa tagaaaaact agtgatagtt ttggtgatat
      361 aagaaaaaca tatatgtata aaaagaagaa agtagcttct ctttgtacat atatggtttg
      421 tttttatgga tgccgagctt ttttataaga taaggctacc gcccataacg acccaaggat
      481 ttgtggaact aagtaaatac ctgcaagaag caagatcagc tgggtcggtt ttaaaggatg
      541 atagaagtaa cgccattcag taaaattggc gcaaaataaa taggaagtga cagtagatat
      601 aatattggca atgaataaag atataatacg cctgttcctt tgaaaataat atccagcaaa
      661 aagagataaa aatatcaatg tgatgaaccc gcttacggaa tgataacgaa aatcaagcag
      721 taaggcgaaa tgcacataag gaacaaaaga taaaagcaac ggaaggtttt tccagcaagt
      781 ttttatcatc tttcatcacc acctaacaag tgttattatt ttgttagttt tattgtaaat
      841 tgaatcctgc aataatcaat tactttttca ttatttctag attaaatgaa taaactctca
      901 tgccattatc tctaaaaaaa aagtttttcc ccctaatttt tttagataga aaattgtata
      961 gtatgatgag agaaaaagta gtttggggga acgttaatga gaagaaaagc agtaatcgat
     1021 cggctttatt tatttttttg gttttttcct atcgttttgc taccttttac atgggtaaat
     1081 gctgttgtac aaggtgcgga gatattaggc ctgatccaag gaagcggatt agtggtttct
     1141 aggaattttc cagctttgac tgttttctat ttagtgataa gtgtaggtct cttattacca
     1201 aaatctctga tcaatcacta caattggcag ctgctttact tttcttgctt attagtattt
     1261 acatctatgt tttctttgct gcctcgcttg attatcggcc ctaatggttt gagcgagagt
     1321 ggattgaagg attcagctta ttcctccatg tatttcttga cgatcaaacc atggttctat
     1381 ttagtaattg cctcatttat tcttgcaaca tttttttact tctggacaga aatcaggata
     1441 aaaagtgaac gatctgtatc tataaaaaat aactattgat ggtggatcat cagtaaaaaa
     1501 gcatatgagc tgcgtaaaat gcatgtttct catatgcttt ttttattatt atgtaaagaa
     1561 tgcgaaaaac gtttacatga tttttacata acttaactat atttttacag caaccatacg
     1621 agggatttac agtcgactga taaactttag ttgtaaataa gaaaggagca ataaaatgaa
     1681 aaaagtattg tttactctag gtatgatggg aattctttta gccggatgcg gctcaggatc
     1741 aggaaataca gatggctctg ccacacaaaa taattcaagt gactcaaatg aacaagtaaa
     1801 aatcgttgcg gtaggttcga cagcattaca gccgttagtc gatgcagcac aagaaagctt
     1861 tgtacaagaa aatccaaatt atcaaatctc tgtacaagga ggaggaagcg gaactggttt
     1921 gagccaagtc gaagctggag cagttactat cggaaactct gatgtatttg cagaagaaag
     1981 agacggagtt gatgcttcaa aattagtgga tcataaagta gcagtggtcg ggatggcacc
     2041 aatcgtcaat aaagatacag atgtcaaaga tatcaccaaa caggaattga ttgatatctt
     2101 tacaggaaaa atcactaact ggaaggaagt tggcgggaaa gatcaaaaaa tcaacgtcgt
     2161 gaaccgtgca aacggcagcg gtacccgcgc gacattcgaa aaatggggac tggatggtgc
     2221 tacccctgtg cagtcccaag aacaagattc atcaggaaca gttcggcagc tggtaagtca
     2281 aacaccagga gcaatcagct atttagcttt ctcatatctc gatgattcca cacaagcact
     2341 aagtatcgac ggtgtagaac caaaagaaga gaatgtagca gataacagct ggggaatctg
     2401 gtcatatgaa catatgtaca cgaacggaaa accttcccct gaagtccaaa aattcttaga
     2461 ctacatgatg acagaagaaa tccaagaagg acctgtcaaa gaattgggct atttaccaat
     2521 cacaatgatg gaagtagaac gtgatcacga aggaaatatc aaataactag cagataaaca
     2581 tgccacggta gtcgttacta ttaaggaaaa atcaactatg tttccccaca tatacatttt
     2641 tcctttaatt gtgaaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg
     2701 tcagctgatt gaacaccgtt tattcgaaaa aaagagctac gacatagaac tgtcgtagct
     2761 ttttttttgc tatatgacag gaatgaaagg tgtttgcaag cttaatgtga ggaacacttt
     2821 ccatatctgt tctatactgg ataggagaag tttggaaata tccagaaaac gaaatcaatc
     2881 gaggtagcca catgaagaat aaatggataa atcgaagtaa aaaaaagaaa gtaacacaaa
     2941 gatcatcaga taaagtcatc cgtctttttc catcgtataa agaaggatta acggcagatc
     3001 aagtcaagga acgaatagaa aaaggtgcag caaataattc agtcgatccc acattcaaaa
     3061 caaatcagca aatcgtattg gaaaacatat ttacgtattt caatttgatc tttcttatct
     3121 tagccatttt gctttgttta gtagaatcct ataagaactt gactttttta ccagttatca
     3181 tagccaatac aggaatcgcc atttttcagg aaattcgatc caaaaagatc ttggacgaac
     3241 taaatgtttt aaatacagca aaagtcaatg tcatacgaga cgggatagaa caaacaatag
     3301 atatagaaga ccttgtaata gatgacattg ttattttaga gactggacat caaattccag
     3361 cagatgcaga ggtcgtggaa gggaaactgc aagttaatga ggctttattg acaggcgaag
     3421 cagatgaaat aaccaaagaa ataggcgatc cattaatgtc cggcagtttc attgtttccg
     3481 gaaaagctta cgcccgatta gacaaagttg gagcagattc gtatatttcc cagctgacag
     3541 tcaaagccaa gacaatggga gaaggagaac agtcggagat gattgcttct cttaatcaat
     3601 tgatcaaatg gattgggatc atcattattc ctatcggtat cctgcttttt tctcaaagtt
     3661 acttttttaa tggaaacacg atcaaagaaa gtgtcgttgc aatggaagca gcattgatcg
     3721 gtatgatacc tgaaggactg tacttgctga cgacgattgc attagcatta agtgcgacta
     3781 aactagcaaa gcaaagagtt cttttacaca atatgaaaag cattgagaca ttggcaaggg
     3841 taaatgtcct ttgtgtagat aaaacaggaa cgatcacaga aaataaaatg tctgttcaaa
     3901 aagtgatcgt ctctaaaaaa caagaaataa tcagtcagtc agatcaattg gaagcatgca
     3961 ttgctgacta tgcaaaagcg atggcaggag acaatgcaac aatggaagct gttaaagcat
     4021 attttacaaa aaccactgat caatcctatc accatgttat tcctttttct tctgtccaaa
     4081 aattcagcag tgtagcgttg gcagacaagg tgtatgtttt aggagcgccc gaaatggttt
     4141 tgcgcgatca attgccggaa tatgcggaag agtttgtttc tttcgctgaa aaaggctatc
     4201 gcgtgttggt ctttggtgtg tacgatggga ttcttgaaga accagtattg acagaagcag
     4261 tcacaccatt aggctatatt ctgattgcta atcctatcag aaaagaagca agagccacat
     4321 ttgattactt caaaaaacag caagtagcca tcaaagtgat ttctggagat aatccgctca
     4381 ccgtatccaa tgtagctgtc caagcaggga tttcacatgc tgatcagtac gttgatgcat
     4441 ctaaattatc agaagaagaa tatgaagcag ctgtcgaaaa atatacagtt tttggacgcg
     4501 tcaagccaga acaaaagaaa atcttcgttc aattgctaaa gcgtcgtaac aatacagtag
     4561 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     4621 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     4681 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     4741 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     4801 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     4861 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     4921 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     4981 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     5041 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     5101 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     5161 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     5221 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     5281 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagtt aaaaaatttc
     5341 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     5401 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     5461 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     5521 tctacagtaa tcgtaccatg aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     5581 ccggtcccgc ctgaatgccg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     5641 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     5701 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     5761 tcaatcagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     5821 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     5881 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     5941 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     6001 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     6061 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     6121 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     6181 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     6241 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     6301 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     6361 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     6421 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     6481 gtatacgccc gatacatctt actcatttgt tcgctcaacg cattgatcgt ctgatacaat
     6541 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     6601 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     6661 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     6721 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     6781 tggccacttt ttttgactgg taatgcgaca tataaaagat ccccattcaa agtagtactt
     6841 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     6901 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     6961 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     7021 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     7081 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     7141 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     7201 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     7261 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     7321 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     7381 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     7441 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     7501 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     7561 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     7621 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     7681 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     7741 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     7801 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     7861 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     7921 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     7981 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     8041 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     8101 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     8161 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     8221 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     8281 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     8341 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacgggagt ttttccggaa
     8401 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     8461 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     8521 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     8581 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     8641 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     8701 aaaaaacttc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     8761 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     8821 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     8881 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     8941 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     9001 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     9061 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     9121 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     9181 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     9241 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     9301 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     9361 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     9421 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     9481 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     9541 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     9601 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     9661 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     9721 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     9781 cgcagcaaga ctatgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     9841 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     9901 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     9961 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
    10021 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
    10081 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
    10141 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
    10201 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
    10261 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
    10321 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
    10381 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
    10441 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
    10501 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
    10561 catatgatcg attcgatatt aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
    10621 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
    10681 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
    10741 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
    10801 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
    10861 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
    10921 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
    10981 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
    11041 aatggctttt tgacactcca acaggtaagc aaaccactta tgaagctttt tatgaaacaa
    11101 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
    11161 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
    11221 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
    11281 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
    11341 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
    11401 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
    11461 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
    11521 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
    11581 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
    11641 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
    11701 tgctgcaagg ttggcacatt caaaaagaca agtgattgtc gtagatggtg gtttgcgcca
    11761 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
    11821 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
    11881 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
    11941 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
    12001 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
    12061 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
    12121 gtcatacgat ttttcctcct tcaaactatc tataacaatc ttacaaaaaa taagtaactg
    12181 gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac
    12241 aaaaaagaag tctaaaatct tctatttcct aaaaaaaatc gaatttaatc aagctgtaaa
    12301 gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat
    12361 ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga
    12421 gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg
    12481 aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact
    12541 tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt
    12601 caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc
    12661 ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc
    12721 tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt
    12781 ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg
    12841 cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga
    12901 tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt
    12961 ttttattaat tgaagaaatg attatttggc aaaacaagca agaaagcgta tccttatagg
    13021 aggagggagt gaacagccaa tgagaaagaa aatgttattt attccatttg ctctattgtt
    13081 aagcgcttgt agttcggtta ctcaagaatc agacaataca catctatcaa gcagtatatc
    13141 tgaaactgtt accagtccag aaaaaagtag taccaaaacg gctaccacca ctgaacaaac
    13201 aatggctacc tcaaataatg agaaaaagac tgctttagac cagttgaaag aacaacagcc
    13261 aaatgttccc atgcctctag atgtaccagt ctcttcaggc tatctcaata tcgcagcgac
    13321 acatacaaag caaggttatt ctattttata ttacagaaca gatcgtcccc tcgggttgaa
    13381 tgctgacgaa ttgaatcaag aaacgcctat tgcaacctat ctatatcaat atgggtttgc
    13441 ttctagtcaa gaaacaatcc aagttttgca gccctttgag atcgacacaa atggtcagca
    13501 agtggactta ggttctcgaa tcacagggta tcagcaaggt gcagcaggtt caagttttct
    13561 tgaatggcaa gaaggaaact ggcgtattcg aatccgtggg aataatatag aaggccaaga
    13621 tcccttacta ttagctaaag aaatcgtagc ttatttggaa gaaaacagtc taccagcacc
    13681 tgaacaattt ggtaaaatta ctgttgatat gggtgatacg acgaatcgag ccgtagaagt
    13741 aagctggcaa gaaccgaaaa atgcctatac gatcactcat caagatccta tgtctgcgtt
    13801 gaagatggca gtttctatga agaggttgtg aaatcagcgc gctgatctga gcccatcatt
    13861 taactccaag tcacaggaag taatcaaaaa tagcacgccg catttttgca taattttggc
    13921 ttaatacctt aaaagaaaac tagaattgcc tcttcacaca tgctaaaata aaacagacaa
    13981 aaaagaaatg gggatataag ttgactgaat acttgaatgt aggaaaaatc gtaaacacac
    14041 aaggtatcaa aggcgaagtc cgtgttatct caacaacgga tttcccagaa gaacgttaca
    14101 aaaaaggaac ggtgttaaca ctattccaag acggaaaagc acctgtggaa ttaacagtca
    14161 aaagtcatcg caaacataaa aattttgact tattgagttt tgaaggacat ccttctatca
    14221 atgatgtgga aaaatatcgg gatggcatat taaaagtgtc aaaagacaac tctgtagatc
    14281 tagctgaaaa tgaattctat taccatgaga tcatcggtct gcatgtatta gaagatggaa
    14341 ctgagttagg taaagtaaaa gaaatcttgt caccaggtgc caatgatgtt tgggtcgttc
    14401 aacgtccgaa aaaagctgat atattgcttc catatatcgc ttctgtcgtc aaagaagtcg
    14461 atttagaagc tggagtggtc cgtgtagaaa ttccagaagg actgattgac gatgaagatt
    14521 gatgtattga ctttatttcc aagaatgttt gaaggtccta tgggggaatc catcatcggt
    14581 aaggctgtag ataaaggatt attagatatc aatatctcta acttccgcga ttattcagat
    14641 aacaaacatc agacagtaga tgattatcca tacggcggtg gagcaggtat gctgctcaaa
    14701 gtccagccca tctatgataa catccaagcc attgaaaaag ctgcaccgga tgtcaaaaaa
    14761 agagtaattc ttcttgaccc agctggtaaa cgattcgatc agaaaatggc agaagaattt
    14821 tcacaagaag aacacttagt attcatttgt ggtcattatg agggatatga tgaacgtatc
    14881 cgcacattag tgacagatga agtttcctta ggagattatg tccttactgg gggagaatta
    14941 ggtgcgatgg tcatgatcga tgcaacagtt cggcttttgc cagatgtgtt agggaatcag
    15001 acttcagctc aaacggattc ttattcaacc ggtttattgg aacatcccca atatactcgc
    15061 ccggcagaat ataaagggat gaaagtacca gaagtattga caaatggaaa tcataaattg
    15121 attgaagaat ggcaattaaa agaatcacta agaaggacct atcaacgtcg gcctgatctt
    15181 ttggaaacac ttgaatggac acctcaaatg acgaaatttt tagaagaaat caagaaagaa
    15241 gaacaagaaa aaacagcaga tgaagccaag tgactttttc taaaatattc tttacagtag
    15301 gttgataata tgttagtatt ttatggtgag tgcaaaagca cttaactatt acgatattcc
    15361 gctatgagaa aagctcataa gaatatttgg aataaggaga agaaaaccat gaatccatta
    15421 atcgaagaat taacaaaaga acaattacgt tctgacatcc cagcgttccg ccctggtgac
    15481 actgtgcgtg ttcacgcaaa agttgttgaa ggtactcgtg aacgtatcca gttatttgaa
    15541 ggtgttgtta tcaaacgccg cggtgctgga atcagcgaaa cttatacagt acgtaaagtt
    15601 tctaacggag tgggcgttga acgtacattc ccattgcata caccacgtgt tgcaaaaatc
    15661 gaagtagttc gctacggtaa agtacgtcgt gcaaaattgt actacctacg tgcattacac
    15721 ggaaaagctg ctcgtatcaa agaaatccgt cgttaatcat tgttatcaga ctgagaaaga
    15781 acccttggta ttcaagggtt cttttttctt agcatgtaat ttgaacaccc tgatccagtt
    15841 tttttcttta attattggta ggggagaata atggattaga ataaatctac ctataagtag
    15901 tagtaaaaaa aagagcacta tgataatatc tttcttaaga gtaaccgctt taataaaaaa
    15961 tccagaatat tctaacataa atggagtatt cttagaatca aaaggagaaa gaagatggtt
    16021 tatacttata cggagcaaga aaaaatgaaa gtttgagaca cattcatgaa tgatggaaaa
    16081 gtaaaacaag taccaagttc aacaaaaaag aaaaacatcc tattgaaaga agtcttgaaa
    16141 cgctttgacc atggagtgac ttatacagaa acagaggtta attcgatact gctgaacgta
    16201 ttttctagtg gcgattatgt cgaacaaaga cgttatctaa tcacatttgg ctttttcaaa
    16261 agaagttcag acggtcgggc atatcagatg atgggaattg aaaattagaa gttttaattt
    16321 atactcattg aaaaaagttc aagtatgcga tattggttct acaccgcata cttgaacttt
    16381 ttttgtcatc tgtatctata atacaaagcc aagaacgtta ttcaaaatat ggatagctat
    16441 gataaaagct aatgagcgat tcgcagttaa ataagcaatc gtcagtaaaa tcgttccagg
    16501 agcgtacatg aaaaaatcaa ttggatgcgt tacatgaata aagataattc tcttttattt
    16561 gtacgaataa ataccaaaac gtacctccta aaattagatt caaagaagat agcaaagagg
    16621 aaaaagatag ttctgcagca gctatagcgg catgtggatt gctagaactt tccaaacaac
    16681 tgcctgtttc agatcctcgt catttcgatt acgaagagat agcaatcaaa atcttggaag
    16741 aattacagaa aaactataca acgaaacgag ttaacggatc aaacggtctg cttctccatg
    16801 ccgtttatga taaaaacagc ttaaaaggtg tagatgaatg tgtgatctgg ggcgattact
    16861 tttatgtaga aggaatcact cgtctagcta aaacatggta ctgttactgg tgatgtataa
    16921 gagaagagga ggattcatat aatgaagcga tggaaaaaaa tgatgattgg atcaatagtt
    16981 ttaggaatgt cactcgtatt tggtgcttgt ggtaatcaag aagacagtag tgaagaatca
    17041 gcaaatgaaa ttaccgtttc gacatggaat tatgaaacaa cacctgaatt caaagcgctc
    17101 tttgaagcat tcgaaaagaa aacgggaatc aaagtgaaag cagtagatat tgcttcagat
    17161 gattatgata cgaaactcac cacgatgctt tcttctggag acacgacaga tgtattgacg
    17221 atgaaaaatc ttttgtcgta ttcaaattat gctttacgag atcagttagt cgatcaaaca
    17281 gaacgaatta gagaattaga cactgatgct gctgaagaaa catatgaaat gtatgatatc
    17341 gatggaaaaa catatgcatt gccatatcga acggactttt gggtactata ttacaataag
    17401 aaaatgtttg atgatgcagg aatcgaatat ccaaaaaatc taacttggga cgaatatgaa
    17461 gatttagcaa aaaaacttag taaaaatgac ggacaagttt acggagcata tcaacatatc
    17521 tggcgttcga caatccaagc tattgcagct gctcaaaacg aggcgaatct tgttgaacca
    17581 gattatcaat atatgactga ttattatgat cgagcattga ggatgcaaaa agaaggcgcg
    17641 cagatggatt ttggcacggc taaatcaacg aaagtaacct atcagtctca atttgaagaa
    17701 caaaaagcag caatgatgta catgggaact tggtatatgg ctggcatact cgcaaacaaa
    17761 gaagcaagta agacagaagt agaatgggga attacagcta ttcctcagaa gaaaaaagga
    17821 gaatcggtca cgacgtttgg ctcaccaaca gcatttgctg tcaataaaaa ctcaaagaaa
    17881 caaaaagcgg cgcaagaatt tattgaattt gcagccagtg aagaaggagc aaaagtatta
    17941 gcaggagtag gagtagttcc ttcatatcgt acggatgaga ttgatcagct ttattttaat
    18001 ctcgctggaa tgccaacgga tgaaatttct aaaaaagctt tttctcctga tgaaatcaaa
    18061 cttgaatttc cgattgatac gtatgggcct gcaatcgata agattttaca agaagaatat
    18121 gacttagttt tggttggaga tgaaacgccg gaaaaaggaa cggtaaatat ggagaaacgt
    18181 gtagctgcgg aaaaagaata atcatgaaag gagagaagcc aagagataaa acactcttgg
    18241 tttcgtcatc gaaatggaga atagtataaa taagaaagct acaaatcatt tgcgtagaaa
    18301 acgaacgtgg atcgcttatt cgtttatcgc cccgaatttc attggttttt tcgtttttac
    18361 attaatacca atcctgtttt cgcttgtact agcatttatg aaatgggatt ctttttcaac
    18421 gccagaattt gctggactag ataactttcg taaaatggcc aacgatgaga cattttggat
    18481 ttctttgaaa aatacttttt tatataccgc tgctgtagtg cctggcacac ttgtttgttc
    18541 gttagggcta gctttactac taaatcagaa aattagaggg atgaagcttt ttcgtacgat
    18601 atttttcttt ccgtatgtta cttccttagt agcaattgct gttgtttgga atatgttgtt
    18661 tcatccaaca gtgggaccta ttaatatctt tttaagacga tttattgaac atcctccagg
    18721 ttggacttca agttcaaatt gggcgctttg ggcaattgta attgtcagta tttggcgagg
    18781 gatgggttat tacatgattc tataccttgc aggactacaa agtatccaaa aagaactata
    18841 tgaagcgtct tcgctagatg gagcaaaccg ttggaaacaa tttttgaatg tgacattacc
    18901 ttcgttgaga ccaacaacct tctttgtaac gatcatgctt gtcatcaatt gtttcaaggt
    18961 gtttgacctt gtgcaagtga tgacggatgg cggccctggt cgagcgacaa atgtgctggt
    19021 ttaccaaatc tacaatgaag cattcaaaaa atttgatttt ggttatgctt cagctatttc
    19081 acttgttttg ttcgtaattg ttttgggcgt tacggttgtt caattttctt ggaataagcg
    19141 tcgtacttag gaggtgtgat atggaagaga gtgttcgaaa tgaaagaaaa aaagcaaaaa
    19201 ttcgttcaaa aaacatgccc tttattcaat ggttgggtat gattctgctg attgtattag
    19261 ctttattgac gatcattcct tttttatgga tggtttctgc ttcattcaaa gcaaataatg
    19321 aagtgttaac gattccgatt caatggatcc ctaaaacttg gcatttagag aattatctgg
    19381 ttatttggtc tcgtattcct ttagtgacat tttttaagaa tacagctttt ttaagcgttg
    19441 tgatcacgtt gattcagttg tttacttcta gttttgctgt ttacgggttt tctgaactaa
    19501 attttaaagg gcgcgatatt ttatttttag catatatcgg cacgattgct gtcccttggc
    19561 agtcttatat gatcccgcag ttcattatga tgagaaaagt gggtttaaca gatacgttgt
    19621 ggtcgttggt gctattacaa gcgttcaatg cttttggtgt gttcttgttg aagcaatatt
    19681 acagtagtat cccagattct ttatgtgaga gtgcgcggat cgatggctta tcagagtggg
    19741 aaatctatcg caagatcatt ttacctctta caaaaccggc gattgccagt ttgacgatca
    19801 tcacattcgt taatacatgg aacgattata tggggccatt catttatttg tcttctacgg
    19861 aaaataaaac aatccagtta gggttgaaaa tgtttattga gctatttgat gcagagtacg
    19921 cattaatcat ggctgcctca gttgtttctg ttcttcctgt tgcttttata tttttagcga
    19981 tgcaaaaata ttttgtagaa gggatcgctt catcaggaat gaaggaatag gaggtgatta
    20041 tgaaagagca gcgttttgac aaaaatgttt atatggaatt cttttattgg ttgtatcaat
    20101 cattggcaag tagcctttgt ttttggtgtg tcaatatctt gtatatagca gctgcgttcc
    20161 ttttgtcgat agatggaagg aatgtctttt cctttatttt ttctttactt tttattggtc
    20221 caggttgcat cgtgatgata ggtatgacag aagaattagc aaaagaaaaa agtttttcac
    20281 cagtacgttt gttcataaag acgtttcgcc aattttggct aaagggattt ttttattggc
    20341 tatttgcttg gatagttagt gtgataatga tttttgattg tttctttttt atacgttttt
    20401 cttatggaaa atggttgatt ccgttgtttg ttttacttgc ttgttttact tgcttgttta
    20461 tcagtttcct ttagcatcaa ctgctggtat tttcaggtga gaaatccagc gtcgaaacca
    20521 aatcaagttc tacggatagc gttctactat acgttgaaaa aatggtatgt gagtttacta
    20581 gattttttac tattgacaag tttgtttctc ttctttttcg taaagcccca atggtgtatt
    20641 ctactaggtc cttctatcgt atttggtttg atttacttca acaatcgtaa attgatgaga
    20701 actatggatc tgtagacttt ataaattgaa actttcaatt aagaatgaca gttttttatg
    20761 taaaaactgt cattcttttt cattttttga aacatatggc taaacaaatg aaataaaaca
    20821 tgctatactt ttgaatgaaa caggtttaga aactgtatca cttatttgtg attatttcta
    20881 actaataatt aaaaataaag cataaaaagg acgatgttca atggctgaaa aaatgaaaca
    20941 aggttcgaaa tctgtagtag aaaggttgat taatcatcat gttgattatg tattcggtat
    21001 accaggtgca aaaatcgatg gtgtatttaa tgaattagag gatcaaggtc ctgaattaat
    21061 cgtgactcgt cacgaacaaa atgctgcatt tatggcacaa gcaatcggac gtattacagg
    21121 agagccagga gtagttatcg caacaagtgg tcctggtgct agtaacttag ctacaggttt
    21181 ggtaacagcg acagcagaag gtgacccagt tttagcaatt gccggacaag taaaacgaag
    21241 cgatctattg aagttgaccc atcaaagcat ggataatgca gcactgttcc agccaatcac
    21301 aaaatacagt gcagaaatcc aagatccaga aacaatttca gaagtgattg ccaatgctta
    21361 tcgtatggct aagagttcaa aaaaaggcgc gagctttatc agtatcccgc aagatgtagt
    21421 ggatgcacct gtccaaggta atgtcatcaa gccattgagc gatcctaaat taggttctgc
    21481 ttctgcagat gatatccgtt acctagctga acgcattaga gaagcaaaac taccggtact
    21541 attagtaggt atgcgaggat caagtgaaaa agaaacgtta gccatcaggc aattagttga
    21601 aaagacagca ttgcctgttg tagagacatt ccaagcagct ggagtgatct cacgtgaatt
    21661 agaagcgcat ttctttggtc gtgtgggttt atttagaaac caaccaggag atatgctttt
    21721 gaaacgaagt gatctagtta ttgcaattgg ctatgatcca atcgaatatg aagcaagaaa
    21781 ctggaatgcg gaaaaagatg cgcggatcat tgtgatcgat gaagcgccag ctgaaatcga
    21841 tccttttatg cagcctgaaa gagaattgat cggtgatatc tcagccactc ttgatttatt
    21901 gacaggttca ttagagcctc aacaagtgtc agaagatgca aaagaatatt tagcatctct
    21961 tcaagcaaaa ttaacggagc gggacattgt tcaatcaaaa ggagaagcag gtatcttgca
    22021 tcctttagaa gttattaaca cgcttcagtc aaaagtaaca gatgatatga ctgttaccgt
    22081 agatgtaggt agtcattata tttggatggc acgccacttt agaagttatg aacctcgtca
    22141 tttgttattc agtaatggta tgcaaacgct tggtgttgca ttgccatggg cgatttctgc
    22201 tgcattggtg cgtccaaata cacagatcgt atcagtttcc ggcgatgggg gatttttatt
    22261 ctctgcccag gatttagaaa cggcggtacg taaaaaattg aatatcgttc atttgatttg
    22321 gaatgatgga cactacaata tggtagagtt ccaagaaaaa atgaaatatc agcgggcgtc
    22381 aggtgtagac tttggtccag ttgattttgt gaaatatgca gaagcttttg gcgctaaagg
    22441 aatccgagct acaagtgtag aggaattaga aaaagcttta gaagaaggat ttgcgacaga
    22501 aggacccgta attattgata ttccgattga ttaccgtgat aatgagaaat tgggagaaac
    22561 aatcttacca gatcaattct attaaggaag tgtcgacatg actgaaaaaa tcttatacca
    22621 gcacgggaca ttaggagctt tgatggccgg cttgatggat ggaacagaaa cgattgctca
    22681 tatattagaa aaaggaactc tgggattggg aacattgcat gggcttgatg gtgaagtcat
    22741 ctttttagat ggcgtagctt atcaaggaag atcagatggc tcagttgccc aattagatgg
    22801 aagtgaactt accccctatg cagcaattac agattttacg ccagatacta gtttttctgt
    22861 ctcaaaaact gcagatggag aacaattgaa aaaagacata ttagtaagag aagccgggga
    22921 aaacctattt ttggcagtga agataactgg attattcaaa aatatgcaca tccgtatcat
    22981 gccgaagcaa gaaaaacctt acagaagact agcaaaaatt tcagagtctc aaccagaatt
    23041 ccaacaaagc aatgtccaag gaacattagt cgggttcttt acgcctgaat tattccaagg
    23101 agttgctgca gcgggatttc acctgcattt tatcgatgat acacatacat ttggcggaca
    23161 tgtaatggat tttgaagtgg ctgaaggaaa agttgagatt agccgcatct cgtcccttgt
    23221 acagcatttt ccagttgatg atccgacttt tgttcacgaa acgattgatt atgctgattt
    23281 agcagcagaa atccaacaag cagaatagaa aaagacagcg agatgcgttc aaggtgatta
    23341 acctgacgta tctcgttttt ttgaggagaa aacctagcgt ttacaaaaaa actgagctat
    23401 aatcttctcc atagatcaaa aaggagcggt ttttatcatg tggacagaaa aacagaaaaa
    23461 agatgcatac gaaattttat tattacagca acaaggatta ttagaagcag aagataactg
    23521 gttagcaaat ttagcaaatt catctgcatt gttgaatgag actttgcctg agactgtctt
    23581 tgcaggatat tatttattcg atggtaatga attgatttta ggaccgttcc aagggcgagt
    23641 ttcttgcaca cgtatcaaga tgggaaaagg agtttgtgga gaatcagctg aaaaacaaga
    23701 aacattgatc gttgatgatg tcaaaaccca tgcaaattac atttcctgcg attcagccgc
    23761 acaatcagaa atcgtagtgc caatggtaaa agaaggtaat ttaatcggtg ttttagatat
    23821 cgattgcgga gtaacgaaag ggtatgatga aatcgatcaa gcatttctag aaaaatatgt
    23881 agatctttta ttagcaaatt ttaatataaa tgctttttca tttattaata actaaaatcg
    23941 tctattgaaa caaaaaagaa gagaaatcaa gaataaaaag agccggaaag acagtgggca
    24001 tttctgtttt tcggtttttt tattttttta taaaatcaca agtgttattg attttcata
//
LOCUS       DAZAID010000038        23828 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000038 DAZAID010000000
VERSION     DAZAID010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.038"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACG9BE_001413"
     CDS             complement(53..787)
                     /locus_tag="ACG9BE_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFN3032162.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACG9BE_001414"
     CDS             1114..1524
                     /locus_tag="ACG9BE_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFN3032163.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACG9BE_001415"
     CDS             1517..2218
                     /locus_tag="ACG9BE_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFN3032164.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACG9BE_001416"
     CDS             2218..3804
                     /locus_tag="ACG9BE_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3032165.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACG9BE_001417"
     CDS             3823..4044
                     /locus_tag="ACG9BE_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFN3032166.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACG9BE_001418"
     CDS             4041..4802
                     /locus_tag="ACG9BE_001418"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFN3032167.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACG9BE_001419"
     CDS             5182..5691
                     /locus_tag="ACG9BE_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFN3032168.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACG9BE_001420"
     CDS             complement(5766..6305)
                     /locus_tag="ACG9BE_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFN3032169.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACG9BE_001421"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACG9BE_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFN3032170.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACG9BE_001422"
     CDS             7318..8169
                     /locus_tag="ACG9BE_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFN3032171.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACG9BE_001423"
     CDS             8197..8832
                     /locus_tag="ACG9BE_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFN3032172.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACG9BE_001424"
     CDS             8852..9625
                     /locus_tag="ACG9BE_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3032173.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACG9BE_001425"
     CDS             10033..10830
                     /locus_tag="ACG9BE_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFN3032174.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACG9BE_001426"
     CDS             10808..11221
                     /locus_tag="ACG9BE_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN3032175.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACG9BE_001427"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACG9BE_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFN3032176.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACG9BE_001428"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACG9BE_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFN3032177.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACG9BE_001429"
     CDS             15998..16558
                     /locus_tag="ACG9BE_001429"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFN3032178.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACG9BE_001430"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACG9BE_001430"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFN3032179.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACG9BE_001431"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACG9BE_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFN3032180.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACG9BE_001432"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACG9BE_001432"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFN3032181.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACG9BE_001433"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACG9BE_001433"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFN3032182.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACG9BE_001434"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACG9BE_001434"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFN3032183.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACG9BE_001435"
     CDS             21905..22483
                     /locus_tag="ACG9BE_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3032184.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACG9BE_001436"
     CDS             22535..23017
                     /locus_tag="ACG9BE_001436"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFN3032185.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACG9BE_001437"
     CDS             23134..23508
                     /locus_tag="ACG9BE_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032186.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23621..>23828)
                     /locus_tag="ACG9BE_001438"
     CDS             complement(23621..>23828)
                     /locus_tag="ACG9BE_001438"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032187.1"
                     /translation="NLTTFENFRHKIFTTNDYYISIPTNNPNNTFSFLTPYPASSPAS
                     RTFFYQKNLYHPIDSTNQPNAVML"
BASE COUNT         7780 a         4286 c         5115 g         6647 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggtat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttt
//
LOCUS       DAZAID010000039        23694 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000039 DAZAID010000000
VERSION     DAZAID010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.039"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACG9BE_001439"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACG9BE_001439"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFN3032188.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACG9BE_001440"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACG9BE_001440"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFN3032189.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACG9BE_001441"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACG9BE_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFN3032190.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACG9BE_001442"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACG9BE_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFN3032191.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACG9BE_001443"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACG9BE_001443"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFN3032192.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACG9BE_001444"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACG9BE_001444"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFN3032193.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="ACG9BE_001445"
     CDS             complement(8557..9027)
                     /locus_tag="ACG9BE_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFN3032194.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="ACG9BE_001446"
     CDS             complement(9073..9498)
                     /locus_tag="ACG9BE_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFN3032195.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="ACG9BE_001447"
     CDS             complement(9565..9834)
                     /locus_tag="ACG9BE_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFN3032196.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="ACG9BE_001448"
     CDS             complement(9845..11440)
                     /locus_tag="ACG9BE_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN3032197.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACG9BE_001449"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACG9BE_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFN3032198.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACG9BE_001450"
     CDS             complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACG9BE_001450"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFN3032199.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="ACG9BE_001451"
     CDS             15906..16880
                     /locus_tag="ACG9BE_001451"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFN3032200.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="ACG9BE_001452"
     CDS             17050..18450
                     /locus_tag="ACG9BE_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFN3032201.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="ACG9BE_001453"
     CDS             complement(18528..19250)
                     /locus_tag="ACG9BE_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3032202.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="ACG9BE_001454"
     CDS             complement(19329..19835)
                     /locus_tag="ACG9BE_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN3032203.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="ACG9BE_001455"
     CDS             19958..21757
                     /locus_tag="ACG9BE_001455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFN3032204.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            22251..23639
                     /gene="ltrA"
                     /locus_tag="ACG9BE_001456"
     CDS             22251..23639
                     /gene="ltrA"
                     /locus_tag="ACG9BE_001456"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFN3032205.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT         6711 a         5100 c         4363 g         7520 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ttgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttgatgtgcg ccctgcatgg gtgaaaactt
    21841 ggtggtgaaa gtccactaca ggcttggcag taggaactgt tagcgaaaga caaggcggca
    21901 atcgtgaggt tgtggctgaa ggaagtcgga cgcaaactcc tgaactgacg aacagaaact
    21961 agatacaagg ctgaattagg tcggataagt cttcatcaca agacaaagtc caatactgcc
    22021 cgaaacctat acagtaaatc tagcagttac atgggaggaa agttttcact cttaccgggg
    22081 gagatctcat gagggtgttg tgggcgttga atgaaaacaa accagcacaa cgatcatggc
    22141 gacatgatgg tgaatcatga gaagtcagcc gaggtcatag tagtaacgac actgaccgtt
    22201 acgaaggact gaacaataag aatcttgaat aatttaggag gtgtgagaag atgcgacgac
    22261 cgcagaaaac aagaaaactt ggctatcatc aaagagctag tgtggaacac gaagggtatg
    22321 atgaagtgcg cagcacttct tgcggtgaac agaggaaaca agatggtgta gaaaatctgt
    22381 ttgggagaat tctagatcgg aataatctca atcaagcgta tcttcaagta gtcagaaata
    22441 aaggggcggc tggtgcagat gggatgaccg tcgatcaact gcttccttac ttgaaagagc
    22501 accgcgagga attactatcc aatttacgcc aagggaacta taaacctcag cctgttttgc
    22561 gagtagaaat accgaaacca aacggcggtg taagaaaact aggaattccc acggttattg
    22621 atcgtatgct tcaacaggca atcaatcaag tgttacaacc gctatttgaa ccagaattct
    22681 cagataatag ttatgggttt cgcccaaatc gcagcgcaca tgacgcagtg aagaaagcaa
    22741 aatcttatta tgaacaaggt taccgatatg tggtggacat tgatatgaaa gcctactttg
    22801 acaccgtaaa tcacgataag ctgatgtatt tcattgagcg gaaagtaaag gacaaacaag
    22861 tcctaaaact gatccgtcaa tacttaatga gtggtgtaat ggaaaatgga ctcatcacaa
    22921 caactgaaga aggaacgccc caaggaggca atctctctcc cctgttaagt aacatctacc
    22981 tgaacgaatt tgatcaagtg ctagaaaatc taggacataa atttgttcga tacgccgatg
    23041 actgcaatat ctatgttaga agcaaacgag caggacagcg tgtgatgaag aaatccattc
    23101 aatttctcga agaacaacta aagttgacgg taaatcgaga aaagagtgcg gtaggaagtc
    23161 cattgaaacg aaagtttctt ggattctgca ttctgccaac aaagaaaggt attaaaattc
    23221 gccctcacgc aaaagcaaaa cagcgtgtga aaagcaagct caaacaactc actaaaagaa
    23281 accgtggaag aagtatccaa cttattttaa aagaaatcaa gcaactaatg actggttgga
    23341 tcaactacta tggaattggt gagatgaaag aattcatgag ggagttaaac ggttggttga
    23401 agcgaagaat cagacaatat atttggaaac aatggaagaa tccaagaacc aaaagaaaaa
    23461 atctgattcg tttagggatc gataaagcga aagcgtatga atggtcaaat acaagaaaag
    23521 gtatttggtc agtctccaaa agtcatattc ttcatcgatc attaacagac aaagaactgg
    23581 cccgccaagg atatgaggac atctccttga agtaccagtt cgtacactca acttattgaa
    23641 ccgccgtata cgggtccgta cgtacggtgg tgtgagaggg gcggaaggtc agtt
//
LOCUS       DAZAID010000040        23150 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000040 DAZAID010000000
VERSION     DAZAID010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.040"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..540
                     /locus_tag="ACG9BE_001457"
     CDS             19..540
                     /locus_tag="ACG9BE_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3032206.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            637..858
                     /locus_tag="ACG9BE_001458"
     CDS             637..858
                     /locus_tag="ACG9BE_001458"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN3032207.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1015..1179)
                     /locus_tag="ACG9BE_001459"
     CDS             complement(1015..1179)
                     /locus_tag="ACG9BE_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFN3032208.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1282..1803
                     /locus_tag="ACG9BE_001460"
     CDS             1282..1803
                     /locus_tag="ACG9BE_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFN3032209.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1790..2230
                     /locus_tag="ACG9BE_001461"
                     /pseudo
     CDS             1790..2230
                     /locus_tag="ACG9BE_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(2270..2428)
                     /locus_tag="ACG9BE_001462"
     CDS             complement(2270..2428)
                     /locus_tag="ACG9BE_001462"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFN3032210.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2521..3282
                     /locus_tag="ACG9BE_001463"
     CDS             2521..3282
                     /locus_tag="ACG9BE_001463"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN3032211.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3469..4821
                     /locus_tag="ACG9BE_001464"
                     /pseudo
     CDS             3469..4821
                     /locus_tag="ACG9BE_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            4837..6057
                     /locus_tag="ACG9BE_001465"
     CDS             4837..6057
                     /locus_tag="ACG9BE_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN3032212.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6092..6769
                     /locus_tag="ACG9BE_001466"
     CDS             6092..6769
                     /locus_tag="ACG9BE_001466"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFN3032213.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6766..7962
                     /locus_tag="ACG9BE_001467"
     CDS             6766..7962
                     /locus_tag="ACG9BE_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN3032214.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7977..8300
                     /locus_tag="ACG9BE_001468"
     CDS             7977..8300
                     /locus_tag="ACG9BE_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN3032215.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8304..8639
                     /locus_tag="ACG9BE_001469"
     CDS             8304..8639
                     /locus_tag="ACG9BE_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN3032216.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8636..9031
                     /locus_tag="ACG9BE_001470"
     CDS             8636..9031
                     /locus_tag="ACG9BE_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFN3032217.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9028..9399
                     /locus_tag="ACG9BE_001471"
     CDS             9028..9399
                     /locus_tag="ACG9BE_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032218.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9415..9975
                     /locus_tag="ACG9BE_001472"
     CDS             9415..9975
                     /locus_tag="ACG9BE_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFN3032219.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10102..10413
                     /locus_tag="ACG9BE_001473"
     CDS             10102..10413
                     /locus_tag="ACG9BE_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032220.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10437..10628
                     /locus_tag="ACG9BE_001474"
     CDS             10437..10628
                     /locus_tag="ACG9BE_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032221.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10628..14473
                     /locus_tag="ACG9BE_001475"
     CDS             10628..14473
                     /locus_tag="ACG9BE_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFN3032222.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14474..15346
                     /locus_tag="ACG9BE_001476"
     CDS             14474..15346
                     /locus_tag="ACG9BE_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFN3032223.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15343..16392
                     /locus_tag="ACG9BE_001477"
     CDS             15343..16392
                     /locus_tag="ACG9BE_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032224.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16396..17220
                     /locus_tag="ACG9BE_001478"
     CDS             16396..17220
                     /locus_tag="ACG9BE_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032225.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17238..19370
                     /locus_tag="ACG9BE_001479"
     CDS             17238..19370
                     /locus_tag="ACG9BE_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFN3032226.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19387..19536
                     /locus_tag="ACG9BE_001480"
     CDS             19387..19536
                     /locus_tag="ACG9BE_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032227.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            19533..19979
                     /locus_tag="ACG9BE_001481"
     CDS             19533..19979
                     /locus_tag="ACG9BE_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032228.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19981..20118
                     /locus_tag="ACG9BE_001482"
     CDS             19981..20118
                     /locus_tag="ACG9BE_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFN3032229.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20155..20448
                     /locus_tag="ACG9BE_001483"
     CDS             20155..20448
                     /locus_tag="ACG9BE_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032230.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20471..20668
                     /locus_tag="ACG9BE_001484"
     CDS             20471..20668
                     /locus_tag="ACG9BE_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFN3032231.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            20679..21698
                     /locus_tag="ACG9BE_001485"
     CDS             20679..21698
                     /locus_tag="ACG9BE_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN3032232.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            21955..22602
                     /locus_tag="ACG9BE_001486"
     CDS             21955..22602
                     /locus_tag="ACG9BE_001486"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN3032233.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(22927..23073)
                     /locus_tag="ACG9BE_001487"
                     /pseudo
     CDS             complement(22927..23073)
                     /locus_tag="ACG9BE_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         8049 a         3925 c         5102 g         6074 t
ORIGIN      
        1 gattaaggag gaagagtaat gcatatagga gataagatac ataaattgag acggcttaat
       61 aacgaaacaa tgaacgactt agttaaacaa accggactgt caaaatcatg ggtatctgat
      121 attgagtctg gaaaaaagca acgcgctgac tttaataaga tatgtgcaat agccaaacat
      181 tataaagtta gcttagaata ctttcaggat gattatttgg atagtgatga acacgaggat
      241 attattcctt tgacagacga aacaagagtt aaatcagtga aatcgcttga acaaagccta
      301 aagtatctga ataacaaaca agaacctaac cttctagaga tcaaactaaa agatactgac
      361 tcagtaccag aggtttatta caaaggtcag aagttagagg gattgataga tgtaagctat
      421 cactaccata caacaactga tgtggtagct ggttcaacaa acgacatcaa tatggagtac
      481 ctaggtgaag taactaagta tccagatata aaaacaatcg gacacaagag agatgtgtaa
      541 atgaaagaag ctagacctag agacgagata gacaaactat acaagaccaa acgatggcga
      601 gacctaaggc aagtagtaat agctagggac ttcggcatgt gccaagagtg caagcgtcga
      661 gggcggaaca caaggggcac gatcatccat cacatagtcg aggcgaggga agacctgtca
      721 ctgttctggt ccgtagataa ccttgaatgt atctgtgtag cttgtcacaa cagagagcat
      781 ccagagagat caggcgggaa gaagaaacca aaacctaaat cacatatcgt taaaatgtat
      841 tcaactcctg aaagataagt ttgcagcgaa atgaaggtag cccccctact ctaaaagatt
      901 aaagagtaag gcttgagaag aacggtgctg tccttccttc gtaaaaagac cgcttttcaa
      961 gttttttgga gaaaaaggaa aaagccgacc aatttaagcc ggctttggac gaagctattt
     1021 cttagtccat ttgtttcctt tttgagaagt aggaggtaat cggtcgcctg gatcaatttt
     1081 tacttctcgt ccgccttgga catttccacc acgaggtccc acttctttat aggttccttt
     1141 tggtttattg tcttcgccgg gtttatagag ttctcccata ggaatccctc cctaaaaaaa
     1201 tttcggcaca gcactgccga taacttaatt ataaggattg tgataacgat ttcaatatat
     1261 cttttgaaag aaggtgatat tatgccgcaa ccagcgaaga gtgcaaaatt acaattatta
     1321 aacggaaacc caaataagaa gaataccgaa gaactccgca agcgagcggc cgcagaagac
     1381 aaattaaaaa tggctactga caaaatcaaa ccgccgttat ggctagattc gctaggaaag
     1441 gatacctttg agtttatcgc tgatgaattg ctgtctgtgg atttaatcag taatccggac
     1501 gtccatacaa tggctctcta ctccaattgg tattcgcaat acgtttcttt agaaaaacag
     1561 cttcgaaaac tacaacgaga gtacaagttg aactatgcgc ttgcgaaaaa ggaggcagag
     1621 gcgagaggtg agccgtttaa tgaacctaat gaattaattg gtaatccgct ctctcggcag
     1681 atggatacag cgtcgcggaa tctccgttct tttggcgctg atttaggact atcaccagca
     1741 gccagagcta agttagctat taagatggct gatgatggtg gtgatgacga tgacgacttc
     1801 taatattttg gatatgtcct acacagaacg tgtggactat tggcaaagct atcttgagga
     1861 gcaagcttct tggggtggct ttttaaaatg tccatatccg gaattgttaa ctacttggta
     1921 tgcggaacga ttaatcgatg gaagcatacc agccagcaaa gaaaatattc aagctgctaa
     1981 acggcatatg cgtgatttgc agcgccaagg aacagatgat tttccttgga tctttgacga
     2041 agaaaaaggt caccggccta ttagatatat cgaaaaaaaa tgtaaaccaa ctgaaggcga
     2101 ctttggttcg tttgttttgc aaccttggca gcatttcata attggatcca tgtacggatg
     2161 ggtacatcgt gatacaggag agcgtcgctt ccgcgaggct cttatttttg ttggacgtaa
     2221 aaacggtaag ttttgccgtt taaaaatcgg gcaaaatcgg tgaaaacctt tatttttggc
     2281 tttcttttag taataattcg atagctttat caagcaattt agacatagga accatagttt
     2341 cttccgacat tttttttaat ccttcgtata aatctttatc aattgcgtta gaaattctaa
     2401 ttctgttttt tagtccgtat ttgtccatgt gtgacactcc tttttatttt actataacat
     2461 ccgattccac cgcttgcaac taccatcaat tgatggtagt atataaaaga ggtgagtaga
     2521 ttgaaaaaat acattattta caaacactta aataaaataa atggcaaaat ttatatcggt
     2581 gtcacaaatg atattggcag aaggtggagg agcggtggga ttgaatataa accaccaaag
     2641 aacgaaaccc aacaccacag aagtttttgg aatgcaatac aaaaatacgg gtgggaaaat
     2701 tttgatcatt tgattattga agaagactta acgatgaaag aagcttttga gaaagaaaag
     2761 ttttatattg aactttatga ctcaactaat aagaaaaaag gttataacat cgcaaaaggc
     2821 ggaaatggcg gaataatata taaagttcac cccaaaggaa tgctagggaa aaaacaatct
     2881 aaagaatttt cttcaaatca cagtaaatgg gcaaagaacc ataagaataa ttgtatgaca
     2941 aacggtgatg ttgtatgggg tgttactcac gaacacccaa aaggcatgtt gggaaagcac
     3001 cacagcaaag aatcaattat gaaaaagaaa gcatatagtg gtgaaaatgc tgtaacctca
     3061 aaaccaattg tcgcaattga acgggatgga actaaaagag aatttcacag cgctaagctt
     3121 tgcatggctt actattcgat tagtacatgt gttttttacc gacttttaaa agatggcgcc
     3181 ccttatgtga taaatcctaa agcgaactac agaaataaag aaaaaatatt agctatcgaa
     3241 ggaattatgt tcaaacataa agaagatacc gaggtaagtt agtagattgc gaaaggttac
     3301 taactaccgt aacgcgtagg agttgaataa atataatgct cccaagagtg tccgacagca
     3361 gataatttgc tgaaaatgta cgctaaactg ggccagaact gactgaccga tgaaaatgag
     3421 ggtgacctcc agagtgcgag ataaaaagct cgcagataat aacaatcgaa aacatcacta
     3481 atttcaggcc tttccacata catggtcgct tatgatgatg aacaaggcgc caacgtttac
     3541 gtattggcaa atgctcgtga tcaagcaagc ttgttgtttg ataaggccgc agaaatggtc
     3601 aaacaatcgc cggcgctctt taagaaattt ggtaagccta aacgatcaag tattaattat
     3661 gctcccgcct tttctaaaat ggaaccacgc gcctcagata gccggaaatt ggatgggcta
     3721 aacactcact ttggtatttt tgacgagatc cacgagttta cgaattacaa tctgatcaac
     3781 gttatcaaga aatcaagagg aaccagaaaa cagcctctga tagtttatat cacaactgct
     3841 ggatatgtat tagatggtcc gttgatgtct tattttgagc aaggtgtgga ctgtttggaa
     3901 catttggaag atgacatcga tgaacggact ttctattatc tggcaaaact tgacagtgcg
     3961 gaagaggctg atgacccaag attatggatc aaagccaatc cgaatatttg tctaatgaat
     4021 tttgttggca tgctagatga ctatgttaag gataaaaaag atccaaaaga atatgctgac
     4081 tggattacca agcaatttaa cttgttttcc gatatcgatg agctgtcatt tgtcgatatg
     4141 cctaccatta aacgaaacaa taaaaccatc gatattgaaa cgctcaaagg taagaagtgt
     4201 gtcggtggtt ttgacttgtc cgaaacggaa gactttaccg cagccgtttt agaatttccg
     4261 cttgaaacag gcgaggtatt cattttgcaa cacacatgga tcccacaagc tagatttgat
     4321 cgagataaca atcaagagcg tatcaaagcg tgggagaagg tgggagatct aacgattatt
     4381 cctggtgatt acgtcaatta tgaatacgtc ttaaattggt ttgtagaaaa ttcgaaaatc
     4441 tatgacattg taaaaatcaa ttatgacaag gccaaggcgc tacgattaaa taaagaacta
     4501 gaaaatgcag gatttgaaac caacgagatt cggcaagggt ttctatcatt aggtgggcca
     4561 atgcaaaact tcaaggaaat gctattggac ggtaaggtga ttttcaacaa ttccaagctt
     4621 taccgatggt atctatccaa cgtcaagctg gtgatggatc gcaactcaaa ctggatgccg
     4681 tctaagcagt ccaagagtag aaaaatagat ggttttgcag caagtttgaa cagccacgcc
     4741 gaagtgttga atatgttggt taatcctgtc ggaaccggga aagtaaccta ttactcgatt
     4801 tccgatttaa tgaatatgta agaaaggtgt ggaggaatga gtattttaga tcgtttgcgt
     4861 tcttttggcc gagcgaagcc gaaagcgagc aaacaagagt attttttgaa tgacccggga
     4921 ttgataccgt atttagtcgg aaaagatgaa atatcagaag ggattttttc cgtaattagc
     4981 cgtgtatcga acgtttttgc gtctctccct ctcaaaatga tagatgcgga gtttggccaa
     5041 ccggacgact gtcctgcata caacttgttg agcgaaggcc ctcgatattt tacaaagttt
     5101 gattttttcc gggacgtgga agttttgaga aactaccaag ggaatgcgta tgtgcagatt
     5161 ttccgaaata tcaatggaga agtagcagat atggcgttaa taaaacctgg tgcttgccat
     5221 ccagtgattg atatggatag cggggagctt tactaccaag taactgcgac tgacaaaggc
     5281 agttataagc aagttattta tgtacattac atggaaatgc tccactttaa acaaccgagg
     5341 tttggtggct tggaaggtac agaccccaca aaagtattaa cgaataccct cggatatgat
     5401 cgagaagtcc gaaaaatctc tttaagtcag cttaaaggag gtaatgaagg gctaaaagtt
     5461 aagtttgcta gcaatgtgga tgaagaagct aaaaaagcta cagttaaaaa cattgctgat
     5521 ttttatcgac aaaacggtgg actacttgtg gaagaaaacg gtgtagaaat cgaacgttta
     5581 caacgagagc tggtagacag caagctttta gatactgata aagtatctcg ctccagaatc
     5641 gcgatggtct acaacgtgcc ggaacatttc attgggagta accagtcgag ttactcttca
     5701 caggaacagc tcaatatgga gtttttgaca tacaatctag taccgaccgt taatcaatat
     5761 gaagcggaac taaataagaa aacactatcg agaaccgaaa aagcaaaagg ttatcgatat
     5821 aagtttaatg tcgcaagttt gctaagagct gacactcagg caagggggca attctatcaa
     5881 atcatgcgac gaggcggagc atactcagcg aatgatgtcc gaagatttga ggacttgcaa
     5941 ccgataaata aaaccggtat ggatgattat catatttccg gagacctata tccaatcgat
     6001 atggatccaa cattaagaaa aacaaactcg tctaaaagcg tagccgaaaa cggttaggct
     6061 tttttagttt gcaccgaagg gaggtgaaag gatgaaaaaa gtgacgttaa gcggcgatgt
     6121 cgtggataac gataccgcgt ggttatatga ctggtttggg attgattgta tctcaccagg
     6181 gaaaatttct gccgctctta cagaagcagc gggggatgaa gtagaacttg atatctcatc
     6241 taacggtggg gatgtcctag cggcaagcga aatatatacc gctatccgtg cctatccggg
     6301 aaaggtatcc ggaaatgttg tgagtattgc agcaagcgct gcaagtgtaa tcgcttgtgc
     6361 ttgcgaaccg cttagaatct cgccaacagc acacatcatg attcacaacg catgggtgac
     6421 cactagcggc aacgccgagg aattaaaagc caatgcggaa atgttaagca gtgtggatga
     6481 gtctatcgtt aatgcttacg agatcaagac aggactcgac cggaaaaagc ttgctgattt
     6541 aatggcaaaa gatacttggt taaacgcaca aacagcagtg gcggaaggtt ttgcggatga
     6601 aattatgttt gcagaagcac cagtaacggt actcaatgcc tctcaaccgg ttattccaaa
     6661 aaacgcggta actaagttga aaaatctaat actcaaagcg gaaacaccgc aagaagaaac
     6721 gctcttgcag aaaaaactaa aagccttaaa tggagggaaa aacgaatgaa tttagaacaa
     6781 ttaaaaaatg cgtgggtcga ggcgggaagt aaagtctctg atttaaacgc gcaactcaat
     6841 gcagcattgg ttgacgatga aaaaacagaa gaagatgtag taagtttgca agcacaagta
     6901 aaagcagcac gggctaaacg ggacggattg aaagagcaag tggcaaatat ggaagccgaa
     6961 caagtcttaa acgtcaaaaa agaaccatta gataaaaaag atgaaaactt gaaaaacaag
     7021 tttatcaaag actttaaagc gatggtcaat ggtgatcctg ctattatggc tactttgaca
     7081 tctaatacgg atgaatctgg taatgctatc ggattgacta ttcctgtaga tgtgcaaacg
     7141 actattcata ctttggttcg tcagtttgac tctttgcaag aatacgtaaa cgttgaaaaa
     7201 gtgaccacta ctagcggttc tcgggtttat gaaaaatggt ctgatattaa cccactgacc
     7261 gctttggata ctgaagacgg tgaaatccca gcaaatgatg atcctgcact ttacttgatc
     7321 aaatacttga tcaaacgcta cgcaggtatt tctacagtaa ctaacagctt gctaaaagat
     7381 actgccgaaa acattttggc atggttgtct aactggatcg cgaaaaaagt agttgttact
     7441 cgcaatacaa aaatcttggc agctattgat ggaatcaaag cagcgcaaaa gaaagatgtt
     7501 acagatgttg atgggattaa agatatcata aacgtccaac ttgatccagc tatcgaagct
     7561 acatctatgt ttattacaaa ccaagatggc ttcaatgttt tagataaagt gaaacgtgct
     7621 gatggatctt acttgttaca aaaagacgta gcttctgcaa ctggatatac cttcttaggt
     7681 aaaccaatca agaaaattgc ttctcgtttc ttgccaaaca aaggtacgca agctactcct
     7741 aaatatccac tgtatattgg tgatctgaaa gaagctgtta cattgtatga tcgcgaaaac
     7801 atgagcttgc tgacaacgaa tattggcggg ggggcttttg aaacagacac caccaaagta
     7861 cgggttatcg atcgatttga tgtgcagtta gtcgatgatg aagcagttgt tttagctaca
     7921 tttacatcta tcgcaaacga agtgccagta acaaacggtt aaggagctga tttcttatga
     7981 ttcttgatcc taaaatggat ttagacgaaa tcaaaaacgc actaaagatt gataccgatg
     8041 atgacgatgt ggaagtaaga cgtgcggtgc aagctgcaat tgcatacatt agaggggcta
     8101 tcgggaatga taagccctct ttttataagc aagaaaatga cacggttgat ctgattaatt
     8161 tagctattct gcaattagca gatcattatt acaacgctcg atcagcaacc gtgagtggga
     8221 acttgcgaga gtacgattta ggttttacaa gcctaatctt gcaactcaaa gcaagatatt
     8281 tgctttttgt ggaggagtag cgtatgcccc ttattcaaac aggaaattta aatcaacgca
     8341 tcaagtttgt ccgagatacg acggtcaagg acgaggacgg gcaagttgtc cggacttcta
     8401 caaccattct tacttgctgg gcaagtgtgc agacacaacg cctgaacgat attaagacgt
     8461 ccattggtac ggctttggaa ggaacactga cgttcattat ccgctatcaa caaaaatcag
     8521 agctaaccaa tgatatgaaa gtgcgttgga atggaaaaac gtttgaaatc attacgatta
     8581 cgaaaggcga gtttgcgaag gacttcacga caatcattgc aaaagaggtt tcaaaatgag
     8641 tgtagaagtc gatgcaaccg aagtgtacaa agcgcttagg gaagtaaaag caaacgttca
     8701 acgagtggaa agcccagcac ttagaaaagc tggggagtac gctcaagaaa agttacaaca
     8761 aaacacacct tactgggatg gaacgaagtc aaacggtaaa cgtggttcgt atatgcaaga
     8821 acatgctaag gaccatgtgg ttacaagctc ggtaaaaaac ggattgatag aagtcggcta
     8881 tgataaagat gtttcttggc ggatgcactt tatcgagttc ggaacaatca aacaacgtcc
     8941 aaaaggtttc gtacaaaaaa cacaaaagca aatcgaaaaa caagtaacac aaatcattgc
     9001 tgacgaagta aaaaggaggc taggactttg aaaacggcag tatcacaagt ctattcaatt
     9061 ctgaatagca atgaaaaaac aaagaacatt gatttttaca ccaatagtgt tccggaatcg
     9121 gctcaaacag tacctagcct tccagttggc agaattacag agatattcgg caactatgaa
     9181 gatttcgcaa gcaacaatcc tttgaccatt caatttaacg tacaggtaga tgtatgggta
     9241 tcgaccttaa aagaggttga tgccttttat tttgcccttg atgagattat gagggggaat
     9301 ggttggcaat gcgcatacac ggaacaaaca gatgatgagg atttagaagg tgcaaagcgg
     9361 attatcaaac gatatgtagc aaatatttca ctaaattaaa aggagagaaa atagatggca
     9421 acagtaggat ttgagagcgt catttttggc gtaaaaacag gtgcaggcgg cactctaaaa
     9481 gaattagtag cagataagtc gaaaggcgga gcgatcgaag ctaaaattac tggattaggc
     9541 gcaacttcta acacaacata cgcttcaaac gtaccattct tcattgcaag taaaggggtt
     9601 tcgtcgccaa aagttacgct tgacgtggca gacttaatgg ataacggcat ttacagcgaa
     9661 atcattggcg ctaaaaccgt ggatggtgta aatgtaattg gttcagaaac tgaagcgcct
     9721 tacgtgtcgg tagtcatggt tacagcgaac aaagaaggaa aacgcttatt catgggattg
     9781 gcaaaaggaa aattcagtca tccagatatc gacatgaaaa cagctgaaga caaaggagtt
     9841 gaattgcaaa ccgattctat cgaaggggaa ttcatttctg atgaacgtgg ctatgtatac
     9901 ttaacagccg tagaatcaga agaaatgacc ttacaaaaat tcaaggactt ggtaaataac
     9961 aaagcggggg agtagttaac cctgcatcta caccaacgac agatacaggg acaccaaaag
    10021 aaccagaacc aaaaattgat acacaaggtt agccattttt tggctagcct tatttttttg
    10081 taaaaacaag gaggaaaaca aatgattgaa ttgcaattga aacttgacgg aaagaaaaaa
    10141 acattcaaac aacaagatat ttccgcacgt gcaatgcgtg agtgtatcaa attttacgag
    10201 aaagcggaaa aagcagacct aactgattta gaagcaattg attcaatgat tgcaattaca
    10261 gcagatattt tccaagatcc agcagttaca tttgatgcta ttttagacgg tttgactgcg
    10321 agcgagttag taccggcatt agaaagtgtt tttgaacaaa tcaatgaact gggaaacaat
    10381 gaaaaaaagc agacggcgag caaaaagaga taagtttttc tgaagctagg aaagcaatgg
    10441 atcaaatcta caaagattta atcgaatcag gttggacgat gagagatgtg gacgaagccg
    10501 actatcatta tttgttacac ctttttggag aagtggagag tggcgaagaa tatgtagatg
    10561 gtgctgattt catcaaacaa tttttatcgg ctgaagactt ggtaaaactt gaggaaggag
    10621 gtaaataatg gcaggaaaag gacaaccggc aggaaatatc aagctaggga ttagtttaga
    10681 tagcactaat tttggtaaca cgctggacga aatcaatgcg aaagtcaaac aagctgagtc
    10741 gaatatgcgt gccaatctaa aggcttatga ttcagcagga cgttcatacg aagcacttag
    10801 tcaaaagacg aaagacttgt ctacggttat ggaagggcaa aacgccaaag taagagaatt
    10861 aacaaagcgc cgtgatgaag cgattagcaa gtatggcgag gaatcgaaac aagttgctaa
    10921 ccttaacaca cagataaaca atgctaccgc aaaatataat gcttacagtc gccagttgaa
    10981 tgacacaaaa aaagaattgg tgtattccaa aacagccgtc aatgatttat ctaacgaaat
    11041 caaagaaaat gaacgacaaa tgaacgccga agtaaaagcg ttgaaagccg ctggtgatga
    11101 atctggtgcg tttgaagcaa aacaaaaagg gctagccaaa caaacggaat tatccgagaa
    11161 agctatcgaa gaacagcgca aagttgtgaa actgatggct gatgagtttg gcgattcagc
    11221 aaatgaaacc gaagatgcaa aaagggcatt agaaaagtta gaacgacaaa gccaaatatc
    11281 tagcaggcaa ttagaagcac tcaaaagctc cagcgatcaa tcaggaaaaa aaatagaaga
    11341 ttttggcgac aagtccacaa ggtcagctag gaaactggat gggctaaaag acaaattagg
    11401 ctcgctaaaa agcgcatttt cgtttggtgc agttgctgga ttagcgcata acgctattag
    11461 cagtgtagta agtggcgtgc aaggcttggt tggcgaagca gtaaacgcat cggattcatt
    11521 gatgaagttt tccaaaacca tggagtttgc taactttggg aagtcacaga tagaaagctc
    11581 gaaaaaagaa atgaaagact acgccgataa gacggtttat ggtttagaag aaattctgaa
    11641 cacaaccgca caattggcat ctaacgggat tcctaactat acagaactaa ccaaggcggc
    11701 aggtaacttg aatgccgttg caggcggttc tagtgataca ttcaaatccg ttgccatgat
    11761 gctaacgcag acggcaggag ctgggaaact aacaactgaa aactggaatc aattagcaga
    11821 tgcgataccg ggcgcttcag ggctgttaca agacgctatg ttgaaaaacg gagcttatac
    11881 aggaaacttc cgtgatgcaa tggcgcaagg tcaaatcact tctgatgagt ttaaccaagc
    11941 gattgtacag ttaggtatga atgacggagc agttaaagca gctacttcca cagatacatt
    12001 gagtggttct tgggaacaga tgaaatctac tgtaataaat gggttacaaa gtatcataga
    12061 caaagtaggc gttgaaaata tcactggttt catcaacaga gtaacaaaag ggattgaaaa
    12121 ttctattcct aaaattactc aatttatagg ttggttgaga gatattggaa cgtggatcgt
    12181 tgaaaataga gagccactaa catggattgt cggaatcata ggcggaatta cattagcagt
    12241 aaaagcattg aacgtagcaa gtatgttgct ggcaattact ggcggaacat tggcagcccc
    12301 ttttgtggcg attggtgtag cattaggcgc actagcaggt gctttggtga tagcttatac
    12361 aaaatctgaa acatttagaa atatagtcaa tgcggctttt acagctgtga aaaacgtagt
    12421 tatgagcgtt atcaataatt tggtggaata ctacaaaatg ttgtggagcg tgttgcagtg
    12481 gctttgggaa aaaataaaag aatgggcttc atggattggc aataaattca ttgaaatgaa
    12541 gaacagcgtt gtgaatacgg ttcagaacct atggaatggt gtgaaaaact tcttcagtaa
    12601 tggcgttgga gacacttgga ataaggtagt tggctgggta aaaaacattt tcaacaaagc
    12661 aactgaattg aagaacaaag tttctgatgc aattggtaac ctgtggaacg gtatcaaaga
    12721 cacattccgt agaggcattg atacggtatt caattggttt tcagaactac cgacgaagat
    12781 gaagaatgcc attattggcg gtaaaaatgc cattgttgat gcgttcaaaa gtattttcaa
    12841 cgcagcactt aaagcgatag gtaaaccagt taacgcaatc atccatggag cttcatgggt
    12901 actagaaaaa ctgggtgctg acaaactcga agaatggaaa gtgccacaat acgcaaaagg
    12961 aacaccaaac ggaggtcatc cgggcgggcc tatgatggta aatgacggta gaggtgctga
    13021 agcggtaatc acacctaacg gacaagcatt tatcccacga gggcgaaatg tagtgttgaa
    13081 tgcaccaaaa ggcacacacg ttctaacagc tgaagaaaca gcttatatga ctggaaacaa
    13141 agcaccaaga tatagatacg ccaaaggtac aggctttttc ggaaatctat ggaacaacgt
    13201 caaaggattt gctggagatg ttggaaacaa gctgaaagat gtagtcggcg atgtatggga
    13261 ttttgtaaca gacccgggag cgttggctag gaaagtgtta aatggtcttg gcgtactgga
    13321 agggcttgtc aaatatcctt tagatgttgg taaaggtatt ctaagcaagg ctaccgaagc
    13381 attgacgaac aaaatcacag aactattcag cagtggcagt ttagacactt caatgggcat
    13441 gcaaggcgtt tacaaatact tggcggacgt tgcagttgca gtaatgaaga agtttccagg
    13501 ctttgtggca actagtgggt atagaccagg tgacccctat tcgcatggta aacgtaatgc
    13561 cattgatatt gcactacctg gtgtcacagg aggctcgcca cgctacacag aagcagccaa
    13621 ttacgcattt gagaagtttg caaacaaaat cggctatgtt atcacaaatg gcaaggttcg
    13681 tgaccgttca ggacaatcag gtacaggtat tcacaatgat tggagaccat ggcccgatgg
    13741 agatcactat gatcatgtgc atttaaacgg tgtgaaagac ccacaaaaca ctcaaatttc
    13801 aggagatagc gtgggaggca gtggggtaga aagatggcgc aatgtagcaa ttagagcgtt
    13861 gaaaatgacc ggtcaataca gtactgcaaa cttaaatgca ttactaaatc aaatgcgtac
    13921 agagtcaaat ggtaatccta atgcagttaa caattgggat attaacgcca aaaatggaac
    13981 accatcaaaa gggttgctcc aagtgattga cccaacattc agacagtatg caatgccagg
    14041 attcaacagc aatatttttg acccactatc taacatctta gcttcaatca gatacgcact
    14101 atcaagatat ggctcactaa caaatgccta tcgtggagtt ggttacgcaa acggtggaat
    14161 tgtaaaccaa catcaaattg cggaaatcgc agaaggaaac aagccagaaa ttattattcc
    14221 tttagataag gctaaacgat caagagcgat gcaattattg tctatcgcaa tggacaaact
    14281 aggagtttct cctaaaactt atggaaatgc gacaactgtt tccagtgatt acgaaatcct
    14341 agaagccatt gaaaaacagg cggcaacaac gaatcagctg ttatcgttat tgctggcatt
    14401 tttcaaagga aatagccgaa ctgatagaga tttagcttta gatattcaga agatcttggt
    14461 taggaggatg tagatgcgaa ctgtattact gagaaataga ataaacgaag aaattgattt
    14521 gtctacggag gattattttg ctactgggtt gagtaatatg gggtttgaag taaaaaaaga
    14581 acatgtggga cagtggggga atttcagaga aagcagtgag aacgttgaaa tatctgaatt
    14641 tcagtcatct gtaatcattt ctgtgcatgg gtttcgagaa aaagaactgt acaattcact
    14701 tgtgcagttc ctatcggaag gtccgtttga actggaattt gcttttgacg gcgaaacaat
    14761 ggtaagaaga tgcagtctaa aatctttaag taagactgaa atcgatccga aaacatcgtt
    14821 actaacagac actttagaac tatatttcac atcaaactgg tattccgtaa aacgcgaaaa
    14881 attaattcag cgaccgaatg tagtgaaaac acgtggtaag gtttttccgt ataaaagatc
    14941 ttatatctac actcaaaact tgtgggagaa aaaaggtgta ttcaaattca ataataattc
    15001 tgtataccta acgaatagca aggaacggat gtccccgcta aaaattcgtg tgattgggaa
    15061 gtgttcaaat ccgtattggg aagtaatcca aaactcacaa atcatcgcaa cggatggata
    15121 cttcatagat atgactgaaa cacagactct agaagtatca agcctttttg aagatacaac
    15181 ggcgatttta aaagatattg caggggtaga atcttctgtc taccaacaac aggattatac
    15241 aaaaactaat tttgttcagg ccccgactgg agaattcagc attgtgtttc atgttggggg
    15301 ggcagacgta gagattgagt tatacgagga gcgtgatctg ttttgatttt agcagtcacg
    15361 ctttttcatc gtgatttaat gctttataac gaacatttat tctctaacag ttttgaattt
    15421 ggtgcggacg agatcaacga agaggctagt agctttacga tggataagta cgttcctgta
    15481 aaaacaggcg attttctact agcaaaatat attcctagtg gaaaatttgc atattttggt
    15541 gtaattacgt cgcaggaaga cgaaaaaatc agttgtaaaa gcttacttag tttagctgat
    15601 agtgagatac cgactgcacg tgtgtcagga gataactacg aagagcatat ccggcggttg
    15661 attgaatact atttgctgaa tgatccaacg aaacaactaa aagatatttt agacgtcaaa
    15721 gcagaaagtg cgacctctca ttcgtatcaa gctaccgata cgaataaaca caagttaagt
    15781 gcgtatattc tcaatggttt caaaaaatac aacgtaaaat ggtatttcaa gggaattcaa
    15841 aacagaaaaa tctatacagg catacgtgct gtaaatgaat caatttacat taaagacaat
    15901 tcttctgaat ttagcgattg ggatgtgttt gttcaggcgc cgggcgctgg aaacgagaac
    15961 aagctattaa tcgttgataa agcaatgaaa gatatagaga aaccgataat actgtcaaca
    16021 tggtatttgg acgaagaaaa caatttgaca caagatggat cgaaagaaaa tatcacgaaa
    16081 ccaactgtca atttggttaa tatctacgat caaaccgcag aagataaagc atcctacgaa
    16141 gatgtggcaa aatcagagtt gaaaggcaat acgtattcgc atgaaatcaa agtgaacgtt
    16201 gtaagaaatg caaaaaattt gaatgtcgaa acgattgaaa cagggatgtt tgccacgatt
    16261 tcttataaag gaaagatata caagtcggtt ttgacagctt ggcgaatatc aagtgataag
    16321 gagtttgtgg aattgacttt cggaaacata agaagtcgtt ttatggatta ttttgaagat
    16381 aatgggggat aaaaaatggt tagcaatgtg gatggatatc aatttgaaaa cgtgaaagta
    16441 agcgcagaaa atgatgctag actttatcac gttttatata atcggaaaaa tcaggttatt
    16501 gatggttacg atcagtctat gaatttatct tcaagcggat taacagtaaa agttgctgca
    16561 ggagcagcga ttattcaagg tcgtatggtc gttgttcgac aagaagaaag tataacagtt
    16621 ccagcaaact caagtggtta tatagcatta acagtggatt tgacacaaga agttatacct
    16681 ggatctattc ttccagaatc ggaagaatat gaatggacta ataatcaagt caagctagaa
    16741 tttataacaa aagttataaa aggtaattta aacaatggtg ataaggtcta taatttacca
    16801 ctatgctcag ttacttctac tggatcaact gtttcaatct caaagatatc ggatagttac
    16861 gagctgactc tctctaaagg agaaattttg tggagtggga ctgcgttaat gcatgatact
    16921 caaactgtcc aaccttcaaa aaaaatgtgg cagacagttt caggtttttt attagtatgg
    16981 ctcccatacg aaaacgggca agcaattggg gatagatatg taactacgcc tttttataaa
    17041 gagcgtgtaa cttttactaa tgttttagga gaaattgttt caggatttga tgactatcac
    17101 aaaaagtggt ttagtaaacg aataaactat aattcgaata ctaatatatt tacaggtgca
    17161 gcaagcaatg caagtggaga taacgcaaat atggtgctta ggtatatagt ttctttttag
    17221 ttaggaggta tggaagagtg gcaaatttag aaattaaatt atctgcaaat aagagacagc
    17281 cttatctacg tcaccgtgtt gttggtagag ttggtgatgg ggggctcaca acaatcaatg
    17341 tacaacttct tgaggaagat gaaattacac cttttgtaat taatctaaac ggtactttga
    17401 aatttgtggg cgaagtttca aacggtaact ataccgaggg agaaccagaa ataatcgatt
    17461 cgactaatgg gttaattagt tacacgttca ctaagtcaaa tttcagcacg agtcatcaat
    17521 tcaaacaagc atattttgaa tatgtagatc ctaacggcaa aaaagtaact tttcagaact
    17581 tcatcataga cgtgttagaa cgagtagata ttaattcgga gcaagcgaaa tactatattt
    17641 cttcattgga aaaattacag agtgaaatgc aaaccacttt caatcagttc attagtgata
    17701 aacaggtcca atacgatcaa atctactcga aatataacga attagtaaga ttgataaatg
    17761 aatcagataa gcaagtaaat gatcgtattg ataagacaaa tgatcgtatt gatcaaacca
    17821 atcagcaaat cggcgatctc ggcaagctga aaaagatgta cagtaacagc atcgacttcg
    17881 gggactatga ttatagtggg aatccgaacc ttagcgctaa acttaatgct tcaagttttt
    17941 ctttaggtac tggcgcaaca gtcgcggatg ataatgatga gattgtgttt accttagatg
    18001 gcacaaatca actatcgaag tacgctagca aagcgcaagt gccggtagca gaagggaaac
    18061 aatatacgat tagttgtgaa attatgttag aagatgggtt cactggcgac ccttcaggaa
    18121 tacgtctgca acacgcatat ttgccaggcg gggttgttgt tttacagaca gagacagtac
    18181 ctaaaaatga attgaatacg tggcagacgc ttatcggaac gcgaacggta aaatacacct
    18241 ctgacgtacc aaatgaatgg taccctttgt ttagagacat ccgaacactt aaaccgaccg
    18301 gtaaagttag actaagaaaa attaagattg aagaaggctc aacagccacc ccatatcagc
    18361 caaacttact tgatgaccct tactggctag gtaaagcgcc tttgggtgag aatattgcta
    18421 ataagtctgt tacgtttcca atcaaatcta gcgcctacag cctataccaa gctaacatgg
    18481 aagaagaatt tgttttaggt caaacctata caattaccat gaaggcaacc aaaccaccta
    18541 tccaaacgtt cattgtttac aacgaggata gtggagatta tagatatggt aacttagagc
    18601 cagtagaagg gttagttgat acatggggtc tgacttttac accgcagaag gttggagtta
    18661 actaccccaa acggttaact attatccagt atccccaatc aacaacaggt gcatgtcaaa
    18721 ttgactggct caagatcgaa aaaggcaata ctcgaacccc gaatattagt gagtataaat
    18781 atcgtggtac tggtatgcgt gattcaaaca atccaaaaga ttatgtttgg gatctagcac
    18841 cagaatatgt cgaagataat ttggccacag atattaaaat ttctgaaatt actggtaaag
    18901 caaacaatta taccgatggg aaagtatcgg agattaattc gcagttgact gcttcaatta
    18961 atgaagtaga caccacagct aaggatgctc aaacaaaagc gaatgctaat gcgactgcta
    19021 tagatgaatt agacaataag atcgatgaac gcattaatga tacagctact accacattaa
    19081 cagttacaaa cgggaatacc ggatcagcaa agctttatcg tgaaggaaaa acagtttcta
    19141 tatattttgt ggctttaaac ggaaaaagca gtggtggaaa tgattcaacg atactaacaa
    19201 ttccagaagg ctatcggcca ccaattagtt ttgagcaact ggttggctcg atagaccgtt
    19261 ctactttgaa cagtgctcag ttatctattg gtgcagatgg agccattaaa tggcgaagaa
    19321 actcaagtta tggatcggat tatacctttg caattactta cacgatttag aaaagcgtga
    19381 atcgatatga aggcagcata tagaccaatt gaaccttacg gattcgagca aatcattgtg
    19441 aatgatgaag aacatttacc ggaagaatgc acagaagtcg aaccaccgat tctaaattgg
    19501 aaaccgaaat tcaattactg ggagggaaat aaatgaaaaa catttggaaa tatggacgta
    19561 ctggcggaga gtacgcagga aaagtattgg acgacatgct tgtatccgtt ccttacacag
    19621 atcagccacc gcttgaagga attcgtgctg atggcgaacc gttaacaatc gctgatcaaa
    19681 tgtttgatcc taaattgaat caatggatta ttttagcgaa cgcactagat cacaacgatt
    19741 taaacaatct caaagcaatg tatgagtcgt tagaaaatga gaacggcgat ttaaaacaga
    19801 tcaatgccaa actcatgcta agcgatgtag caattaaaca ggaaaatact gcattgaaag
    19861 aaaaagcgga tagtttagca caaatcaatt caaaaatgat gcttgcttcg ttacaaaata
    19921 gcaaagacat ttcagaaatt aaagagcaac taaatccagc ttcaaaggga ggtgagtagt
    19981 atgtttagtt ttagcgatgt gaaaatgatg tatgattggg gctgttttac tgacgatcaa
    20041 gttcgactat tcgttccact atgcattaca gacgaagaag cagataaaat cattagcaaa
    20101 gaagaaagcg catcttaagt gatgcgtttt tatttaaggt aaaggagttg tcacatgatc
    20161 aatttagggg aatggggaac aatcgcagga tcaattactg cgattgtttc tttgatttta
    20221 ttagtaataa gaccagtcac tgcatctttc tcgaagatta ctgaaactct ttcaaaagta
    20281 agtcacaatt tagatttgct gactaaagat ttagaatcga gcaaatcaga tcgattgatg
    20341 attcatgaag aactaaagaa acacgatgaa agattagata cacatgcaga aaaattggta
    20401 gaacacacac aacaaattaa aactttattt agagaaaggt ctcggtaaaa aagaaaggag
    20461 ttaagaagaa atgattttac ccgataagta ttatcaagtc attaaatgga cagttttaac
    20521 agtattgcca gctttatctg tattagtagc cacattaggc aaagcatatg gatggaatgg
    20581 aacagatatg gcagttctga ctatcaatgc agcagcaaca tttttaggtg ttatcactgg
    20641 tgtgtcggct tataatttga aaaaatagga ggaaacaaat gaaaaagaaa attactatta
    20701 ctgcgatgag cctattaacg gctctttttt tattgccgat taatgggttt gcctatacga
    20761 ttaacaatga atttaattta ggtgtaaacg aaggtagttc tcaagtagca aataatcagt
    20821 acattttact gcatgaaacg gctaatgaaa cagcaacagg acgcaatgaa gcgcagtata
    20881 tgcaacgttc atggactagc gcttatactg cttatattgt gggagacggc ggaattgttt
    20941 atcaagtcgg tcaacctggt tatgtacagt acggtgctgg ttcgtatgct aatgcaaata
    21001 gtcctgtgca gattgagtta caacacacac atgataaagc aacgtttgag aaaaactaca
    21061 aggcatacgt tgaattagct agagattcag caatgaaata tggtattcca cttacactag
    21121 acacaccgta taatcaacca gggatcaaat cgcatttatg ggtaacacaa aatatttggg
    21181 gcgatcatac agatccttac ggttatcttt ctgaaatggg cgtaagtaaa gaaaaattag
    21241 cctatgattt ggctcatgga tttaccgatg aaaatccgac aacttcagat gataaaccag
    21301 tcattgatcc aactcgagca ggtgctgcaa atcctaccct gacagatgga acaaattacg
    21361 cccacattga tcagttcgga gaaatcgaaa acgcaaactt acatgtggct ggttggcaca
    21421 tcgctaacta taaatacgag tatatcttca ttatggatta caatactggg aaagaattag
    21481 ctcgagtaaa agctgatgga atatatagac cagatgtaaa tcaagcttat aatactttag
    21541 gaaatgttgg ctatcatgta tcttttaaca tgcgcaactt ccctaataag aaagtctatg
    21601 taatgatgcg ggcaacgaat gatccagaag ggaacactaa aggcggagca caagattttc
    21661 atgataaacg ctggtattta aatattccgc aacgataaaa atagcccctc gtttttgagg
    21721 ggaagtacat aatgcaaatt tgtccataac ttattgatta tcttgtaaaa atgcggtaat
    21781 ttaatagtta gtgagaagag gtgcttaatt atagttaaaa aagaggaaat agaagcagca
    21841 tgtaaccaag caaatgttag gtttattgga gaagctttat ctggatatcc taagtataaa
    21901 tacttacaca actggttaca aagtaaaagt ttttatctta ataaagaaag atgcatgcct
    21961 aataaaaatt atagggttta taaaagagga actattgtat atgttgattt tggggtgaac
    22021 gtgggttatg agttatctgg taatcatttt gctattgttt taaataataa agataattcg
    22081 aaaaatggtg ttgtatgtgt agtaccaatc tcttcaaaaa agaaaagtaa ttatgtatct
    22141 gtaggaaaaa tattagaagt tgctagtatt aaacaattta taagccaagc tgataaatta
    22201 aaagagaaac ttaggataat tattttactt tcattaaata gaggcatcat tgatgaaagc
    22261 gatttgaata gttttattaa agatattgaa gctaaaggtg aaaagctttc agtggaagaa
    22321 gtagaaaaac gagctgtaaa acataatttg gtttgcgaga caagggaaca agtaattaaa
    22381 gaaataaaat cattatctga agatatggta aaatatcaga aggtgtttaa ttgctacaag
    22441 aaatatacac aagaatccta tgtaatgcca cttaatatcc aaacgattag taaaaataga
    22501 attagaagaa ttaacaagtt tgatccttct ggaaatatga aggcacctgg aaatgtcctc
    22561 aatgaagtag atgaaagttt aaaaaaatat tttacaaagt aaacgatatt tttgttgaca
    22621 tttttctgtt cgttagttac catgtagtta tagaaggacg taagcgccta tttttaatgt
    22681 acacatcggg acgtatgcgc ccacatagaa acatagttga gagacatcta tatggtgtct
    22741 ctttttttat taatatgcaa aaaagtctag ttttttcaat aatatttatg taataataaa
    22801 ttagccacca caacaaagaa tgaaatccat tattatctag tctagtccat tctttttgtt
    22861 tgcagtattt gtgatggctt tccgtaccct tagctcagtt ggtcagagca gacggctcat
    22921 aaccgtccgg tcgtacttct cgtaatccag gaaggccgtc agctccgtcc gcagtagcgt
    22981 gtttacggcc agctccagat gggagcggaa taactcgttc aaatcacctt tggtggctag
    23041 agtctgcagg agttctgtaa taaaatcgtt catagggaag tcctcttttc tgtgaatggg
    23101 tgtggtaact ttattctaca gaagggactt ccttttttct atttacacaa
//
LOCUS       DAZAID010000041        23010 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000041 DAZAID010000000
VERSION     DAZAID010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.041"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACG9BE_001488"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACG9BE_001488"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFN3032234.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACG9BE_001489"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACG9BE_001489"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFN3032235.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACG9BE_001490"
     CDS             8118..9086
                     /locus_tag="ACG9BE_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3032236.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACG9BE_001491"
     CDS             9104..10009
                     /locus_tag="ACG9BE_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3032237.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACG9BE_001492"
     CDS             10006..10812
                     /locus_tag="ACG9BE_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3032238.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACG9BE_001493"
     CDS             10969..11937
                     /locus_tag="ACG9BE_001493"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFN3032239.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACG9BE_001494"
     CDS             complement(12254..12778)
                     /locus_tag="ACG9BE_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFN3032240.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..14876
                     /gene="scm"
                     /locus_tag="ACG9BE_001495"
     CDS             13065..14876
                     /gene="scm"
                     /locus_tag="ACG9BE_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFN3032241.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(15031..16017)
                     /locus_tag="ACG9BE_001496"
     CDS             complement(15031..16017)
                     /locus_tag="ACG9BE_001496"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFN3032242.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16040..16606)
                     /locus_tag="ACG9BE_001497"
     CDS             complement(16040..16606)
                     /locus_tag="ACG9BE_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFN3032243.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16620..17036)
                     /locus_tag="ACG9BE_001498"
     CDS             complement(16620..17036)
                     /locus_tag="ACG9BE_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFN3032244.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17493..19427)
                     /locus_tag="ACG9BE_001499"
     CDS             complement(17493..19427)
                     /locus_tag="ACG9BE_001499"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN3032245.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19729..20664
                     /gene="pplA"
                     /locus_tag="ACG9BE_001500"
     CDS             19729..20664
                     /gene="pplA"
                     /locus_tag="ACG9BE_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFN3032246.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20754..21857
                     /locus_tag="ACG9BE_001501"
     CDS             20754..21857
                     /locus_tag="ACG9BE_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFN3032247.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21870..22823
                     /gene="menA"
                     /locus_tag="ACG9BE_001502"
     CDS             21870..22823
                     /gene="menA"
                     /locus_tag="ACG9BE_001502"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFN3032248.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT         6760 a         4866 c         4503 g         6881 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta
    14161 ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact acctctacca
    14221 caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc tctaccacaa
    14281 gtgacagcac ggacacgtca gcttcaagcg acagtacaga tactacctct accacaagtg
    14341 atagcacgga cacatcagct tcaagtgaca gtacagatac tacctctacc acaagtgata
    14401 gcacggacac gtcagcttca agcgacagta cggatacatc taccacaaca agtgatagca
    14461 cggacacgtc agcttcaagt gacagtacag atactacctc taccacaagt gatagctcag
    14521 attcatctac cactacaagt gatagcacgg acacgtcagc ttcaagtgac agtacagata
    14581 catcctctac cacaagtgat agctcagatt catcaacttc aagcgatagt acagacacgt
    14641 cgtctaccac aagcgataca cgtcatgcaa gtgtctcctt ctcaaaaaca agttcggata
    14701 acaggacgaa ctttaactta actaataaaa caagcaatga caatggaaac aaatcaaaaa
    14761 gagcacttcc taaaactgga agtcaatcga ataattggat aacactcgca ggcgttatat
    14821 tacttgtaat cggtttaaga atcactttta tgagttataa gaaatcgaaa aggtaaaaaa
    14881 atattcaaga taaaaaagag caacgtcgct aacggacgtt gctctttatt tttgcttttt
    14941 atcataaata caagaactac aattcaattt gtcaaaaaac acgtagattt ccttctgaga
    15001 aatttattta cgtatcgaac ctttccctat tcagtctttg cgatccaaaa tcagttgggt
    15061 aagggaaaag agattctctt ttgttcgaag agaggtggca ggtaatttct ttatttcctt
    15121 caaagccttt tctgtgtaag aggccgctaa cttcctagct tcttccacgc ctcctgtatg
    15181 ttcaacgatt ttgcgtacgg cgtcgacatc ttcttctgtc attgccgctt tcttctctaa
    15241 ataaggtagt agctttggtt tgtttttttg caaagagtag atcaacggta aagaatagac
    15301 cccttgacgc atgtcttcta gtacaggttt gccgatttcc tggctctctt ttgtataatc
    15361 taaaatatca tcgataatct gaaaggccat cccgatagaa agtccgatat ctcctgcttt
    15421 ttttgcgaag cgctcgcttg ttccactttc aaaagcgcca acagaacaac ttaattggaa
    15481 cagttcagct gtcttacctg aaatattttc caaatattct tttacagaaa gttcataatt
    15541 atagcgatca tccatctgcc ctaattcgcc attcaatact ttttccatac tgcgagaatt
    15601 caattggaga cttttcaagg aagtggagta atcagacaat agtttaaaac agcagacaaa
    15661 taaatagtct cctgcataga cagaaacgac attaccaaac tgactacgga tattaggctg
    15721 accacgtcgc agatccgctt catcaacgat atcatcatgg atcagtgtgg ccgtatggag
    15781 catttcgata gaagctgcca aagcgatggc tttgtctcga tcttgctctg gtccaaattg
    15841 tgagaacaat agctgatagg caggacgcaa cagctttccg ccggcatgga tcgtttcttt
    15901 gattgcttgt tcgacaggtt tgtttttcag gcggacagtt tcttccatcc gttttagtgt
    15961 ttctgtcaat tctttactta aagcaggata gttttcccac attggatgta gcttcataaa
    16021 aaaactcctt gtgtgacgat tattttttgg taaacattgg gctccatttt tgtttggttc
    16081 cctgctcata atctttttgg aatcgttgaa gtgtctgcac atgtcgtaaa agataattcg
    16141 ctgcgatccc gattgcaatc cctgataaaa tccctaaaac tgaaaggact ggcaagtaca
    16201 acatgactgt ccatgattgt gcaatgaaag aagccatcaa aagctgtccg acattatgca
    16261 tgaaaccgcc ggccgcacta attccgatga tactgacacg ttttggtcct aattgcttga
    16321 ccaataacat tccaaaataa ctaagcaaag caccactcat actataaagg aatgtggaaa
    16381 tcgttccgcc tagcaaagta gtcaggaata gtcgtagcca aagtagtaag aagctgtctc
    16441 gttttttcat ggtaaaaatg gcaacgatcg tgattagatt ggctaagcct agttttgcac
    16501 caggcgcaaa agaaaaagga taagggatca tattttccaa taaaccaatg atcactcctt
    16561 gtgcaacaag tagcgaaata taaatcattt tttgtaattt actcatggtg tgtgaaggtc
    16621 taataaatca gactgtcttc gtcacctcca tctgttgaac ggacttcgat cactaattta
    16681 tgtggcaaac agacgatggt ttcgccattt ttcgttgccc agcctcgacg tacgcagatc
    16741 tggtcatcgc aatctgcttc ttttatccgt actcgatcgc catctatctc gatcaagtta
    16801 taatctccgt gttcatcttt gtaagtatat gtgttgctct cttctccagc catcaaaggg
    16861 aatgttttga tttctgtgcc atccacacgc agtaccgctt ctttttctgc tgttgcggtg
    16921 ttttgctgaa gaccaaaaat catgactggt aaaaaagaaa gaacagtcaa aagcaaaata
    16981 ataaaaacat cccaaggttt gagcagactt ttttcagcaa attctttaag gctcattttt
    17041 gttctccttt ttccagatta actcacccaa ttttagcata aaaacaagag gttgtgacac
    17101 ctgtgtcttg ctttgagtaa taagaacgaa gaatagaaaa cagctttcca tgttttctaa
    17161 ttctttgggc ttattaccga gaagcagcag gtggaacacc gtttataaca agaggttgtg
    17221 acagaagtgg gcaacttcaa gaaataagaa ggaatttacg aaaattactt ttcaagtttt
    17281 tgtgaaattt cggcttattt ttcgaagaag ctacttctgg aacaccgttt ataacaagag
    17341 gttgtaacag aggttgtgac acctgtgtct tgctcatgga ggcttatctc gggcatctta
    17401 ttcctttaat tttacataaa aaggaggctg tgacggtttg tctcagcccc tttctattga
    17461 aatcttgcat tcaaataacg ttctttatct atttatttct tgccgtactg ggaacttggt
    17521 ctaccgtacc accaattacc tagtgtacgt tgtagccatg gaattaccca gcgatccaac
    17581 ccaatcgcac gtccggaacc gttcatcaat gcgaaagcaa caaaaatgaa ccaaatgttt
    17641 acccagtaaa acatgcctga taagcagaac gtgatcacca atacaatcgt tgtcgcactg
    17701 catagccatg tgaataatcc agcaatcaat gctaatgcca aggcaatttc aaagaatacc
    17761 atgaattttt ggaagaacat tgctacatca ttgtttggca tgatcacttg catgatcttt
    17821 gtgaaccagt ctggtgactt ctcaatcaca gccattggtt cttcaccgta tgcataactc
    17881 aaaccaaaat gtgcagcttg agtcgctgct tctccggcac ttgcccctgc tccgctggca
    17941 gctgtggttg catcaccagc agcagaagca ccactggcaa catctgttac attgtattcc
    18001 catgtccaag ggaacacact gtttccagtg aaccatgatc cttcaccaaa ccaagtactt
    18061 ggtttgaaca cttcaccatt cccaatgatt tttttggaag cttcaacgaa ccaaactaaa
    18121 ccatagaaaa tacgaagtgg cacactccat aagacattgc cgtaacgaga agaatgtcct
    18181 cgagtcacgt tgcgatcatc tttgatatgg aaaaactcat gcattaaata ttggaacata
    18241 taataaccag aacgaatatc aaagaagtat ttcaaattca cgatatgttt cattacgata
    18301 gctaaaaatc cgcttaaatg gatcttatca aataggttag ctacccccca tttggcacca
    18361 atagaaacca taaatccttg atagtttcct ttaaatttat gcttttctcc gcctttgatc
    18421 gaagccacga tattcgctgc ggctgtgtgt cctgtttgtt cagcagcttg aacgatctgc
    18481 ggtgtcggtg ttcctgggaa ttcttcataa aagactaaat caccaatgat atagatgttt
    18541 ttatcttcgt agccttttgc ctgcatatat tcattggcga tcaaccgctg tccgcgtgct
    18601 gattctaatc caaagtcagc agcgtcagaa gttcctttga caccagctgt ccaaatcagt
    18661 gtgtgagtcg gaatgttgcg tccgtctttc aatttgatat gatcagcagc cacttctacg
    18721 attggtgcat tcaataataa ttccacgttg tgcttacgca aatagcgttc agcttttgca
    18781 gcatcatttc gatccaacat attcaagatc gtcggcattg cctctacgac catcagtgtg
    18841 atttcgtttg gatctaattt ataatctttg gctaggcgat ttttccagtc gatcagttcc
    18901 ccaatcattt caataccggt aaatccagaa ccacagacaa cgaaagtcag catggcttta
    18961 cgcagttcat catctggttc caatgcagct tttgctactg tcttctcaat atgttcccga
    19021 atccttacag aatcttcaaa tgaccaaagg gtgaatccgt gtgctttgac accaggtgta
    19081 ccaaagtcat tcggctctcc gcccattcca aggatcagct gatcaaaagg ataggaacct
    19141 aatttggtct tgacgatttt tcgttcttta tcgataccga ttacagtgtc tgtgacaagt
    19201 tgtacattct tttgacgagc aaacaaatgc tgcagatcgt attggattgc ttctggttct
    19261 acacgtccgc cagctacttc atgcaactcg gtcatcatag tatggtacga atgacgatcg
    19321 atcagagtaa ttgctacatc ttcttttttc agtttctttg ccaagtattt tgtagctgaa
    19381 acaccggcat atcctgctcc tacaacgaca atattttgct ttgccattga tcatccgctc
    19441 cttatagact atctatataa tgaatatatg ataattcttt tagtttgata cttattacac
    19501 aatctccata tattatatag atgttctgtt cttttgtcta actccttttg gatttttttc
    19561 aaattttaaa ataaagcgtt ttttcacgaa atttgctgaa agaaaacgtt tgaaatatta
    19621 catgggttat ttatcaaaaa tagttgactt ataggctaaa aagattagaa tgtataatga
    19681 atcacaaaga aaaattgtgg actagaaatg gaatggtggg gtattaaaat gaagaaaaaa
    19741 cgttttgtaa ctggatttgc cgtattagca ttttcagctc tagtgttagg cgcttgcggc
    19801 gcagatgaca acggaagctc aaactcttct tctgaatcat ctacagcaca atcttcgact
    19861 gcaaaaagca caacagaatc atctgcaaaa gttgtagctg gcggagattt gcaagacggc
    19921 acatataaat tagaagaaaa aaattattca aatggttacc gtgcagtctt tgaaatggtt
    19981 gtaaaagacg gcaaaatcac tgaatcaaaa tatgataacg tcaatgaaaa cggcgaatca
    20041 aaaacaaaag atgcagaata caacaaaaac atggaagcaa aatctggcac aaacccagaa
    20101 aaatacattc ctgagttgaa tgaccaattc ttaaaagcac aaagcgcaag cggtgtggaa
    20161 gtcgtaactg gagctactca ctcatctgaa agcttccaaa actacgcaca acaattgatc
    20221 caagctgctc aagctggtaa cacagataca atcgaaatcg acaatggtgc tgatttgaaa
    20281 gacggcactt acaagcttga agaaaagaac tactctaacg gctaccgtgt gcaattcgaa
    20341 atgacagttg ctggcggcaa agtaactgaa tcaaactttg attatatcga caaagacggt
    20401 aaatcaaaac aagatgatac agaatataat gaaaatatga aagcaaaaag cggtacagaa
    20461 ccaaaaactt acatccctac tctgaacgac gaattcgtca aagcaatggg cgaagaagat
    20521 ggctcacctg ctaatgtaga agttgttaca ggtgctacac acagctcaca ttcattcatc
    20581 atgtacgcac aacaattagt gaatgcagct gaaaaaggcg acactcaaac aatcgaagta
    20641 gataatatcg taacagaaaa ataatcacat aatcatacta ataaaagttg gagggtggga
    20701 cgatagccag attaggcagt cgtctcacct ttcaaaatga agagaggaaa aagatgcgga
    20761 aaaaactact attttcctta atcacactgg gcatctcttt tatggtgcta actggctgca
    20821 gcaactcagc aaataaaaca accactgaaa gttcagcagc taccaagata ttgaaagatc
    20881 catattctga tgaacagttc ttgttaggga cttacgttcg tatccgtgtg tatgatgaag
    20941 gcaaagaatc agcattgaaa cctgccttcg atcgagtaaa agaacttggt gataaaatca
    21001 cgatcaatca aaaaggttct gagatcgatg aagtgaatga gcaagctggg atcaaaccag
    21061 taaaagtttc tgatgacgtc tacacgttag tcaagcgggc ctacgaatat agccaagatt
    21121 ctcaaggcgg attcgatatg gcgattggtg caatcacaca attatggcga atcggttttg
    21181 atgacgccag aaaaccttcc caagaagaaa tcgatcaggc attgaaatta gtggattatc
    21241 ataagatcga actaaatgac aaagaaaaaa ctgtttatct aaaagaaaaa ggaatgatca
    21301 tcgatttagg tgcaatcgcc aaaggttaca ttacggacga agtagtgaaa gtcttgaaaa
    21361 aacaaggagt gaccactgcc atcgttgact tgggcggtaa tgtctatgtc ttaggccaca
    21421 gcccaagagg cgaaaaccaa gactggacag tcggcatcca agatccgaac atggctcgcg
    21481 gatctgtttt aggttctatt aaagaacgga acaaaacatt agttacctct ggaatttacg
    21541 agcgttattt agaagtagat ggcaaaaaat accatcattt gtttgatcca aaaacgggtt
    21601 atccgttcga caatgatatc gcaagtgtca cgatcataac agataaatca attgatggtg
    21661 acgggttgtc tacagcagtc ttttcaatgg gtgtgaaaaa aggactggaa tacgtcgaaa
    21721 gcgaattgaa taacgggacg gaagcgatct tcgtgacaaa agacgataaa gtatatgtga
    21781 ccgatccaat caaagacacc ttcaaattat cggaggactc ccattacacg atgggtgacc
    21841 gttctgaact aaaataagga gtacgaacca tgacactaaa aatcttttta gaagtagttg
    21901 aaatacgaac aaaggtggct agtgtatttc cttttgcggt aggggtatta ttctctatcg
    21961 ctttttttca tgagatacat tggctaaaca cttttttatt ttttattgga atgctagttt
    22021 ttgatttagc gactaccgct atcaataatt acatggatta tgaaaaggct cattctgaag
    22081 tatataagta tgaagaaaat gtcattggcc gcagaaatct atctcctgta ttgattcgaa
    22141 acatgatttt agctatgatt gcatttgttt ttgttatcgg cgttattcta acgatcaata
    22201 cgggttggct tttacttttg atgggattta tctgttgttt tattggagta ttctatacat
    22261 tcggaccgat tcctttatct cgaatgcctt taggggaaat tttcagtggg gtgactatgg
    22321 gccttggaat ttttgccatg gtcgtttatg tcaatacatt cggttcagat gtctatgatc
    22381 tgacagtcaa cttttcatct ggacaatttg ctttggtagg aaatctttgg gctatagcag
    22441 caatcgtatt agcttcctta cccttggtgt ttacgattgc caatatcatg ttggctaata
    22501 atttgcggga tcttgaccgt gatattgaaa atcatcgcta tacactagtt ttttatatcg
    22561 gacgtcctgc gggaacagtc ttattccagc tattgatgta tgcttgctat ttcgtgattt
    22621 taatggggtt gttgagcggt atctatgaat ggccgatctt ggtcaccttc ttgaccttgc
    22681 caattgtgaa gaagcatttg gatcaattca agcaaacgtt accccagcct atcagtttca
    22741 gctattccat caaaaatatg gtcttattca atgccagtta tgtattgggg ctattactaa
    22801 caatcattat gcaagccatt taacagcatg aagaggttgt gaaaaaactg ccctgcatca
    22861 agtaataaga acagccaaat aaaaatagct ccttatattt ttcgtttggt tgggcttatt
    22921 accgcagatg caagcttttg aacaccgttt atcaggaggt tgtgaaaaag tcgcttagtt
    22981 tcgagtatta agaaaaattt tgtgaaaatg
//
LOCUS       DAZAID010000042        22853 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000042 DAZAID010000000
VERSION     DAZAID010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.042"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..671
                     /locus_tag="ACG9BE_001503"
     CDS             153..671
                     /locus_tag="ACG9BE_001503"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN3032249.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            799..1482
                     /locus_tag="ACG9BE_001504"
     CDS             799..1482
                     /locus_tag="ACG9BE_001504"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFN3032250.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1791..1864
                     /locus_tag="ACG9BE_001505"
     tRNA            1791..1864
                     /locus_tag="ACG9BE_001505"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1825..1827,aa:Arg,seq:tct)
     gene            2023..2382
                     /locus_tag="ACG9BE_001506"
     CDS             2023..2382
                     /locus_tag="ACG9BE_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFN3032251.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2505..4136
                     /locus_tag="ACG9BE_001507"
     CDS             2505..4136
                     /locus_tag="ACG9BE_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFN3032252.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4198..4839)
                     /locus_tag="ACG9BE_001508"
     CDS             complement(4198..4839)
                     /locus_tag="ACG9BE_001508"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFN3032253.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5025..5101
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5121..5429
                     /gene="rplU"
                     /locus_tag="ACG9BE_001509"
     CDS             5121..5429
                     /gene="rplU"
                     /locus_tag="ACG9BE_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFN3032254.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5456..5800
                     /locus_tag="ACG9BE_001510"
     CDS             5456..5800
                     /locus_tag="ACG9BE_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFN3032255.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5813..6106
                     /gene="rpmA"
                     /locus_tag="ACG9BE_001511"
     CDS             5813..6106
                     /gene="rpmA"
                     /locus_tag="ACG9BE_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFN3032256.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6540..9347
                     /locus_tag="ACG9BE_001512"
     CDS             6540..9347
                     /locus_tag="ACG9BE_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN3032257.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9538..10029
                     /locus_tag="ACG9BE_001513"
     CDS             9538..10029
                     /locus_tag="ACG9BE_001513"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFN3032258.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10132..11121
                     /locus_tag="ACG9BE_001514"
     CDS             10132..11121
                     /locus_tag="ACG9BE_001514"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFN3032259.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11154..11957
                     /locus_tag="ACG9BE_001515"
     CDS             11154..11957
                     /locus_tag="ACG9BE_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFN3032260.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11978..12892
                     /locus_tag="ACG9BE_001516"
     CDS             11978..12892
                     /locus_tag="ACG9BE_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3032261.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12975..13349
                     /locus_tag="ACG9BE_001517"
     CDS             12975..13349
                     /locus_tag="ACG9BE_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFN3032262.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13488..13733
                     /locus_tag="ACG9BE_001518"
     CDS             13488..13733
                     /locus_tag="ACG9BE_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN3032263.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13905..14969
                     /locus_tag="ACG9BE_001519"
     CDS             13905..14969
                     /locus_tag="ACG9BE_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFN3032264.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15215..15640
                     /locus_tag="ACG9BE_001520"
     CDS             15215..15640
                     /locus_tag="ACG9BE_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN3032265.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15633..16082
                     /gene="nusB"
                     /locus_tag="ACG9BE_001521"
     CDS             15633..16082
                     /gene="nusB"
                     /locus_tag="ACG9BE_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFN3032266.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16179..17033
                     /locus_tag="ACG9BE_001522"
     CDS             16179..17033
                     /locus_tag="ACG9BE_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFN3032267.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17034..18371
                     /gene="xseA"
                     /locus_tag="ACG9BE_001523"
     CDS             17034..18371
                     /gene="xseA"
                     /locus_tag="ACG9BE_001523"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFN3032268.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18376..18600
                     /locus_tag="ACG9BE_001524"
     CDS             18376..18600
                     /locus_tag="ACG9BE_001524"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFN3032269.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18602..19492
                     /locus_tag="ACG9BE_001525"
     CDS             18602..19492
                     /locus_tag="ACG9BE_001525"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFN3032270.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19501..20319
                     /locus_tag="ACG9BE_001526"
     CDS             19501..20319
                     /locus_tag="ACG9BE_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFN3032271.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20426..20875
                     /locus_tag="ACG9BE_001527"
     CDS             20426..20875
                     /locus_tag="ACG9BE_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN3032272.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20893..22575
                     /gene="recN"
                     /locus_tag="ACG9BE_001528"
     CDS             20893..22575
                     /gene="recN"
                     /locus_tag="ACG9BE_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFN3032273.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22630..22746)
                     /locus_tag="ACG9BE_001529"
     CDS             complement(22630..22746)
                     /locus_tag="ACG9BE_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFN3032274.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
BASE COUNT         7451 a         3791 c         5036 g         6575 t
ORIGIN      
        1 aattatttat aataaaaaat gagataaaga tgaggaaata gaattagaaa catataatta
       61 ttaaagggaa acatttttac agctataatt tgaaaaaatt tatcgttatt atatgattaa
      121 gaaggattga ataaagaaag aagttgaaaa cgatacctga ttacaaaaaa ataactttta
      181 taagttctat aacgttgatt atatttttaa ttcttattct tctcgtcgtc ttggttcccc
      241 gaatttttgg tttgactcct tatattattg aggacaaaga aatgagtcca tattatgcac
      301 aaggaaatct gatatatgta cgaccgaaaa atacaaatga gattttagtc ggagatatta
      361 ttacatatta tgaaaattca ggaaaaaaaa tctcgacgcg tcgcgtaata gccgttgaaa
      421 acaaccacga agatttttat acaaaagctg atacaaagac ttatattgag cctggagtag
      481 taaaaaagag aaatattatc ggtagtccca tctttcagat tccatatatt ggactagtcc
      541 tttcaaaaac ggcatttgca tggattcagg ggttattctt tacgattgct ttttgcctaa
      601 caggaatcac agcatggaat acctttgtag agttgaaaaa gaagcggaga cgaacacaga
      661 aatttttgta agaatgttaa aaacataaat gtattccaga gatccaacga ctcttctgaa
      721 catgaaacac gagaaaacac tcatgcagct tgtagattta gtcagttgtt cgtataatgt
      781 gtgaagagga ggagtagtat gcgcaatgaa atgatgcatc gtcctgtagt caaagaagaa
      841 ctattggatt ttatgcgtac ccagcaaaaa aaattaccgg gtgagctagg caaactggaa
      901 gaagaagctc atgtagcaga agtaccagtt attccccatg aaacagttgt atttttgaaa
      961 tttttacttg gacagcttca gcctgaacga atcttggaga tcggagcagc tatcggtttt
     1021 tcatcaagtg tcatggccac gacaatcgga gaaaacgctc atgttacgac aattgatcgt
     1081 ttcgatgtca tgatcgagaa agctaaaaaa aactatgaac gtctaggact gacagataaa
     1141 gtgactttgc ttgaaggtca agcagcagat atcttgccgg agttgtctgg tccgtatgac
     1201 tttattttta tggatagtgc aaaatcaaaa tatattgaat ttttacctga atgcttacgt
     1261 ttgttaagaa aaggtggcgt tttgatggta gatgacatct tccaaggagg aacgatcttg
     1321 ctcccagatg aagagattcc ccgtggcaaa cgagctatcc atcgtaaatt aaatgaattt
     1381 cttcgtgttg tgatggatca tcccgatttg acatcgacaa tcttgccatt aggtgatggt
     1441 gtgatcttaa tgacaaagga atcagaaaca atcgagttgt gatacatgat tatggcagcc
     1501 tgtagataat cgattgaatc gagggctcaa ttcgaacttg atgtaaaagt ttctatattt
     1561 gtaagtcaat taaaggatac aggaaatcta aataaacagg agtagcatga agtcaatgta
     1621 gctcctgttt tcaattgata accttagaac agctgaagga gaatgtctcg ttttaaaaga
     1681 ttgatttagt agttgggcca aaataattca ttatttgcta attaaataac tatttcgtat
     1741 tgacagaagc aatttaatta tctataatta ttgatgttcc gctgattctt gtcatagtag
     1801 ctcagctgga tagagcatcc gccttctaag cggacggtcg ggggttcgaa tccctcctgt
     1861 gacgctaaaa aaccgttaga aactgaggtt tctaacggtt tttctttgtt ttttgtgcca
     1921 atattgtttg ttttcaaagg caaataaaat aaaaaaacat ccttatcgag tacaattata
     1981 aggagcaaaa aaataaaact catcagaaaa gaggaagaag atatgacgat actatctaga
     2041 attttagtga ctctagttgc aattgaattt ttttatatca tgtatatcga aacggtgaga
     2101 actgattctg acacgacaag tagggttttt aaaatgagta aagaagagtt aagcagaaaa
     2161 tctgttcaaa cattgtttaa gaaccaagga gtgtacaatg gactattagg agtgggacta
     2221 ctttatggag cttatctttc ttccgcatct aaagaaatca caagtatgct attaatatct
     2281 attttttttg ttgcattata tggcagtttg actagcgatc gttggatttt actcaaacaa
     2341 ggtgggctag ctctgttggc attgctttct attttcttat aaatatagaa aaaataagaa
     2401 tttatctaac aggaaacttt gtgaaaaata ttactttatc tcatgaaata tgatattcta
     2461 atgaatggta ttttagaaaa tttcatgaaa aaggagttag ataggtgttc aaaaattatg
     2521 tttcctcact aaaaaaagaa ttttcaggtt atgatctttc aagatttcag aaagatcttt
     2581 tggctgggat cacagtagct gctgtagcat tgcctttagc cttggctttt ggggtatcaa
     2641 gcggagctga tgcggccgca ggtttgatta cggctatcat tgccggtatc gttattggcg
     2701 gtttatcagg cggtttttat caaatatcag gtccgacagg tgcgatggca gctatcttga
     2761 tgtctattgc ggcaacaaag ggaatgcaag gtgtattgct tgctactttt ttagcgggtc
     2821 tatttttact tgcagcagga cttttccgat taggaacact gacttctttt atcccagcac
     2881 ccgtaattac ggggtttact tcaggtattg ctatcattat tgcattaggt cagatcgata
     2941 atttatttgg tgttcactcg gaaggtacaa acgttattga aaaactaagt agttatcaaa
     3001 cgataggatt tccgataaac acagcctctt tgatcatggg agggctagtc attttaggga
     3061 tgtttatcta tccaaaaaaa tgggcgaaac gaatgcctag ttcgctgctg gctatcattt
     3121 tggcaacagc agttatgcta ttgttccatt taccagtagc aactgtgggt aagattccac
     3181 aaacattgat cagtagtaat cgtttgaata tgggagattt tagcttttct gccttgcaac
     3241 aagtggcagt tccagctatt agcattgctt tattaggaat gattgaaagc ttgctatgtg
     3301 gtgcttcggc tggccgaatg gcaaataagc ccttagatag caatcaagaa ttaatggcgc
     3361 aaggaattgg aaatctgttg cttcctttct ttggcgggat tcctgctact gcggccatcg
     3421 cccgtacaag cgtggccatc aaatctggtg cacaaactag attagctggt atgatacatg
     3481 cagttgtctt actgctctcc atgcttgttt ttgcgccagt gatgtcacag tttccaatgc
     3541 cagcacttgc aggtgttttg attgtgacgg cttggcgtat gaatgaatgg gaaacgatca
     3601 aggagctatt tactaataaa tattggtcag cagttctatt gtttatcctt acgatgattt
     3661 gtacagttat ctttgattta agtattgcta tcgtgatcgg ggtgatcggc ggctgtgtat
     3721 tttttgttgt caaaagcgct gctatcacga tttcattaga aacgatcgat tggcaaagaa
     3781 tggggctgcc agaaacggaa aagctgaata actggacagt agtgtatatt agtggtccac
     3841 tgttttttat gtcttcagaa aaactcaaaa ataaattgga agaactaaat catcacgatg
     3901 gtgtcatttt ctccatgcga ggcgtaccaa gtattgattt gacggcacgt gcagtttttg
     3961 atgaatttca agaaaaagca gagcaaagag gacaaacgat tatctatact tccttgcagc
     4021 cagaagtaga aaaacaactt tctcacttgt gggaacatac attcacgcat caacatcaaa
     4081 cagttgctca tgcgttgaca gctttacatg cgaaatatac tgcaattagc cattaaaaca
     4141 atgaaaaaga acagacaaga ctgtaaactg aagattttat ggtcttgtct gttcttttta
     4201 atattgctgt aaaagtgaag ctagtacttg ccagtcttga tggataacag gtacaaggga
     4261 gcgattgtag aaaatagctg ctggatgaaa aagtggaaga attcgatagt ttttttctga
     4321 ccattcgtag gcatcctttt gttctgttaa ttgtaagaca ggccctgtat agagttgacc
     4381 atgatttttg gtaaccgtat attctttgcc aagtaaacgt tgaagaccaa tgtttccaag
     4441 tgtcgcaatt aattttggct ggaccttcat cagttcgtaa tccaatattg gtgcatgcgc
     4501 aaatacttcg gaacgagtag gtgtccgatt tggataaaca gtttctgact gttgtgtccg
     4561 tttattgatc cgatgagtca cgcgatacgg gcggctcctt acagcactgg tgatatacac
     4621 ctcttctcgt gttaagccag ttgaagaaag tgcctgcatc aattcttttc cagcttgacc
     4681 gctaaaagga acatgcgtat caatctctgt tttccctggg gcttctccca cgagcatcag
     4741 tttaggatga agaggtcctt gcccagcgat aaatccttct acaggacggc cgttcattct
     4801 taaagaagct tcttgtatca gttcttttgg gtattccatt tatgctcctc ctttttcttt
     4861 tattataaga ataaaagaaa cacaatgcga ctattttatt tggtagaact gtgaggatag
     4921 aaaagcagaa gtattgcttg aatttttgtt ttactttttg attttaaatt tagttgacta
     4981 acggttgctg ttctggtaag ctagtactgt tgtaattgtg gactccacag ctacaaccgc
     5041 tcagaacaag taataagcac agcatatgct gtcacttcgg atggcgagtc taagtatatc
     5101 agaaggaggt gctttatagc atgtacgcaa ttattaaaac tggtggtaaa caagtaaaag
     5161 ttgaagtggg acaagcaatc tatgttgaaa aacttgacgt tgaagcagga gaaaaagttg
     5221 tttttgacga agttatctta gtaggcggag aatctacaaa agtaggcgct ccaactgttg
     5281 caggtgcaac tgttgaaggt actgttgaaa aacacggcaa acaaaagaaa gtcgttactt
     5341 tcaaatacaa acctaaaaaa cattcacacc gtaaacaagg tcatcgtcaa ccatatacaa
     5401 aagtggttat cgacgcgatc aacgcttaat agcgtttgag aaagaaggcc tatatatgat
     5461 caaaggaaca tttaagcgaa atgacgcagg tcagatcgtt tcattcactt tgaccgggca
     5521 tgctgatgct ggtccttatg gaagtgatat cgtttgtgcg ggtgtttctg cgttagcgat
     5581 cagcactgtc aacgggattg cctcattagc cggttttgag ccgattgttg aaatgaatga
     5641 agaagaaggc ggttatcttt ataccgaagt cacttcagga atgacacaag aacaaaacaa
     5701 tattgctgag attctattag aaaatctttt attaggatta caatcgatag aagctgaaaa
     5761 ctcagaatat atccaaatta aaacaattaa tgataaatag gaggtgcaga ccatgttgtt
     5821 atctatgaat ctacaattat ttgcccacaa aaaaggtggc ggttctacat ctaacggccg
     5881 tgactcagaa tcaaaacgtc taggtgctaa acgtgctgat ggacaaacag ttacaggtgg
     5941 atcaattctt taccgtcaac gcggtacaaa aatctatcca ggtgtaaacg tgggtatcgg
     6001 tggagacgac actttatttg caaaagttga tggcgtagta cgtttcgaac gtaaaggccg
     6061 taacaaaaaa caagtgtctg tttatccagt agctcaagaa gcttaattga atcttatgat
     6121 taaagaggtt gtgacaagac ttttgtcaca gccttttttg tttataaaaa aaaaagttgt
     6181 agtggaggct acgaagttta ctttgtcttt ttacacttaa tatttttttg ccaatttgga
     6241 atcaggatgt tttgataact atcgttttca attgatggaa gctatattgt ttgctatcag
     6301 tgaaataatt atatggtaaa cagtgttctt tttcttgttt ccattataaa aatcgtatcg
     6361 ggatttttcg ttcttttaaa ttgaacattt ctatctatcc gcgtgttatc aacaaaacaa
     6421 aatcaggtaa atagttgacg aaacagaaat aaatacatag tattatgttt tgaagcctaa
     6481 gtacaatagt ctatagaaaa ggagcgatac cattggcaaa acgcattgac cgaatttata
     6541 tgtatgtcaa agaaaaaacc gctcatttaa caaaggcaga atatgatcaa ggagtaacaa
     6601 cacaggaaat tgctgaagtg ttagggattc agcgaacaaa tagcagtaaa gatctgaatc
     6661 agctagtgcg cgaaggaaaa ctgataaaga cagacggacg accagtacgt tacatatatc
     6721 aaacactcgt tacgcacgta aagccagtta caaaacatgt agtcagttac aaagaaacgc
     6781 cggtctctga agaaaaaaat acactccaaa tagatactaa ggatatcttt gcaaaaatca
     6841 ttggagctaa cggcagcatg aaaaattcag tggaacaggc aaaagctgcg attttgtatc
     6901 cgccaagagg attaaattgt ttgattacag gacctaccgg atcaggaaag acttattttg
     6961 cacacgcgat gtatcatttt gcaaaagcgc atcatgtaat agatgaggaa acggaactga
     7021 ttgttttcaa ttgtgctgat tatgccaata accctgagct tttgatgagt catttatttg
     7081 ggtacgcaaa aggtgctttt acaggcgcag atgaagaaaa aatggggatt atcgatcaag
     7141 cggatggcgg tatgctgttt ttggatgaga ttcaccggtt gccgcctgaa ggtcaagaaa
     7201 tgatctttta ctttatggat catggaactt acagtcgtct tggggaaaca acgaaatccc
     7261 atgaagcaaa tgtacggatc gtaggtgcga ccacagaaga cccaggttct tctctattag
     7321 aaacttttgt tcggcgtatc ccaatcaata tcaaacttcc ggcttttgaa aaacgtcctg
     7381 caaatgagaa agtcgattta gtaaaaatca tgattgccca cgaagcaaac cgcacgcagc
     7441 gaaagatttc tttgacagaa gatgtagcaa aagcattgat cggcagtgtg acctatggaa
     7501 atataggaca attgaagtca aatatccagt tagtatgtgc acgaggattt ctaaatcata
     7561 tgcaatcacc cgaaatctcg atcacaatcg atgatttgac agaagggatt cgtagcggat
     7621 tgattcaatt agcaagcaac cgcagtgcaa tggcagagct ttctaaaata ttggagccaa
     7681 agataactgt agcaccaaat gatacgatca tgaaaatcca atcggactcc tatgagttac
     7741 cttataatct atatgatatc atcggagata aagcagcttt actgaagtcg gatggtttgg
     7801 atcaagaagc aatcaatcat ttcatttcta cagatatcaa catccatctg aagtctttct
     7861 ataaagacca tggtttttct tttaatgcag acagcaaatt agcagaattt gtagatccga
     7921 aaatcataga agtgaccaat cagatttatg taatggtcaa aagatcttta ccatatgagt
     7981 ttcagcaaaa ctttatttat gctatgagct tgcatatcag ttcttttttg aaaaaaatca
     8041 acttaggaga agaacggcat acgaatgata acatccgtga gatggctttt gattttccag
     8101 atgaatatgc tgttgcgaaa gaagtgcgta gttatatcga aacgtatttt caagtggaga
     8161 ttccagaaag tgaagactat tacttgaccg ttctactcgt ctcgttacgg gcaaatcaag
     8221 caagcggcag aatcggtgta gtcgttgcag ctcacggaaa cagcacagca agcagtatgg
     8281 tccaagttgt ccaacagttg ttagatgcag agaacgtacg agcagtagac atgcctttgg
     8341 atatggatcc gaaaacagca ttggaccgaa ttgaacgcaa tgtacaagaa gtagatgaag
     8401 gaagcggggt catactctta gtagatatgg ggtcccttgc gtcgtttaat tcgcaaattc
     8461 aaagagacac tggaattcca gtacgtactg ttgatatggt aacaacttct ctagttttgg
     8521 aaactgtacg aaaggtctca gtattaggga cagatctaga tatgttgcat gaatcattga
     8581 aaaattttcg aggttacgca gaaatatctt ctgtagaatc tgttcaggaa gcaaaaccta
     8641 ttcataagaa aaaagctgtt ttggctatct gcgcatctgg cgaaggtaca gcacaaagaa
     8701 tcaaagaatt gatcgaacga gcggtaaaca agcgtcaaga aacagaatta gaagttctag
     8761 ctttgtcgat tgtggaagtg aaagaagaac tgccagttat ccaaaaagaa tatcagatca
     8821 tagctactac tgggattact gatttgaata tccaagcccc atttattcct ttagaacgtt
     8881 ttattgatca aaatatcgaa gtcattttgg atcagttgct gatggaatca gaattggagg
     8941 aaactgaatt catctcccta gatgaagagt cagctaaaaa tacgtgtgtt gagtttatct
     9001 ctgataactt catctttatt aatggtagta agttaatcga tccaatgtgg caattttcta
     9061 cacaaatctc tcagacgacg ggaatcggcg atgaagaata tggattcaaa atcaacctag
     9121 tgatgcacac agcaggaatg atcgaacgta ttattcggaa tgaaccattg actgtggaag
     9181 aaaacgaatt gacaaataca acgaatgatc ctctttatag ccaattggca gcctcagttg
     9241 ttctgttgga agatcagata aaagtaaaag tgccgataga agaaatgtat tatctgctac
     9301 gtctagtcca taatcagtta gataaaaaag aatacactgt gccatgaata cacaaaaacg
     9361 atacactttt atagtgtatc gtttttgtgt attggttaag aatcctttaa attcaattag
     9421 tgtatcgtaa aaaagttggc acgctttttg cttatagtag tgtgtgctag gaggtggttg
     9481 ttcacacaga aatgataggg gtttctgtga agtagtgagg aagaaggagt tttgcagatg
     9541 gatattcgat tggcacgtat agatgatcgt ttaatccacg ggcaagtcgc aacagtttgg
     9601 tcgaagcaaa caggtgttga acgtatcatt gtgatcagcg atgtagtcgc tcaagataaa
     9661 ttgagaaagt ttctactaaa agaagctgca ccgccgggga tcacagcaaa tgtaataacc
     9721 gtgcagaaaa tgattcaaat ctatcatagt ggattattat cacgagcaaa agtgatgctt
     9781 ttattcacca atccccaaga tgttgaagcg gttgtccgct gcggtgtaca gttgaagtcg
     9841 ttgaatatcg gcggtatgag ttttactgaa ggcaaatcaa tgattaccaa ttttgtttca
     9901 gtaaatcaac aagatattga ttcctttcgt tacttgaaca gtcaagaagt ggagttagag
     9961 attcgaaaag tacctgctga caggaaaatc cttttgatgg aactactagc gaaaatcaaa
    10021 aaggtgtagc aagaatggtt gttggaaaag gtcaaataat tctgttgtaa tggttagcaa
    10081 ttattaatat ttttgaattt gttacaacaa aagtaaatag gaggtataaa tatggtaggg
    10141 attatcctag caagtcatgg acaatttgct gaaggaatct tacaatccgg ttcaatgatc
    10201 tttggcgaac aagaaaacgt caaagccgtg attttgaaac caagtgaagg tccagatgac
    10261 ttgagagcca aattggaaga agcggttgct tcttttgata atcaagacga agtgttattc
    10321 ttagttgatt tatggggcgg aacaccgttc aatcaatcaa acacattatt cgaagagcac
    10381 aaagataaat gggcaatcgt cagcggtttg aatttaccaa tgttgattga agcctacgca
    10441 tcacgctttt caatggaatc tgctcatgaa attgcagcac acatcattga aacagcaaaa
    10501 gacggtgtaa aagtaaaacc ggaagaatta gaaccggcag aagcaccgaa agcagcagtt
    10561 gaagacgcac aacctaaagg agcacttcca gaaggaactg tggtcggaga cggaaaaatc
    10621 aaatatgtat tagcacgtgt tgactcacgt ctgttacatg gacaagttgc gacagcttgg
    10681 acaaaagcag tgcaaccaaa ccgtattatc gttgtttcag atgcagtatc aaaggatgat
    10741 ctgcgtaaga gactaatcga acaagctgct cctccaggtg taaaagcaaa tgttattcct
    10801 attagcaaaa tgatcgaagt agcaaaagac cctcgttttg gaaataccaa agcattgctt
    10861 ttatttgaaa atcctgaaga cgtattgaca gctgttgaag gtggcgtaga catcaaagaa
    10921 ttaaatgttg ggtcaatggc gcattcggtc ggtaaagtcg ttgtaagtaa agtattgtct
    10981 atgggacaag aagacgttga agcgtttgag aagcttgaac aaaaaggtgt gaaattcgat
    11041 gtacgtaaag tacctaatga ttcacgcgat aacatggatg atattttgaa aaaagcaaaa
    11101 gctgaattag caaaagcgta ataaataata ttaaaattta taggaggctt atcatgtcta
    11161 ttatttcaat aattttagtc gtattagttg cctttctagc tggtatggaa ggtattcttg
    11221 atgaatttca atttcatcaa ccactagtgg catgtacatt gatcggttta gttaccggta
    11281 acctcgaagc tggtatcgta cttggcggta cgcttcagat gatcgcttta ggttgggcaa
    11341 atatcggtgc cgctgtagca ccagatgctg cattagcatc agtagcatca gcaattattt
    11401 tagttttagg tggacaaggt gttaaaggtg ttccttctgc gatcgcgatt gccgttcctt
    11461 tggctgttgc agggttgttc ttaacaatga tcgtacgtac aatcgctgtt ccaatcgttc
    11521 atttgatgga tgcggctgct gaagaaggaa acatccgtaa ggttgaaatg tggcacatca
    11581 ttgctgtatg tttacaaggg gttcgtatcg cggtcccagc agcagcgttg ctatttatcc
    11641 cagcacaaac tgtgcaatca ttccttgaat caatgccagc atggttaaca gatggtatgg
    11701 ctatcggtgg gggaatggtc gtagctgtag gttacgcttt ggtaatcaac atgatggcta
    11761 caaaagaagt atggccattc ttcgttattg gtttcgttgt agctgcaatt tctcaattaa
    11821 cattgattgc tctaggtgct ttaggtgttg cattagctct tatttacttg aacctttcta
    11881 aaatgggtgg ttcatcaaat aatggaggcg gaagcaactc tggtgaccct ttaggcgaca
    11941 ttttgaatga ctattaatct tgaaggagga aaataaaatg gcagaagaaa aaatcaaatt
    12001 atcaaaaaaa gatcgtttag ctgttgcgtg gcgctctacc ttcattcaag gttcatggaa
    12061 ctacgaacgt atgcaaaacg gtggctgggc attttctatg attccagcaa tcaaaaaatt
    12121 atacaaaaca aaagaagaac gttctgcagc attaaaacgt cacttggaat tctttaatac
    12181 acacccatac attgcttctc caattttagg ggttacattg gctctggaag aagaacgtgc
    12241 gaatggcgcg cctgttgatg atgtagcgat tcaaggggtt aaagtaggta tgatgggtcc
    12301 tttagctggt gttggtgacc cagtgttctg gtttacagta cgtccaatgc taggtgcact
    12361 aggtgcttca ttagctatgg gaggaaatat ccttggacca atcatcttct tcttaggttg
    12421 gaacattatt cgttgggcat tcatgtggta tacacaagaa tttggctaca aagctggttc
    12481 taaaattact gatgaccttt ctggcggatt gttacaagac gtaacaaaag gtgcttcaat
    12541 tttagggatg ttcgtattag ctgcccttgt acaaagatgg gtatcaatca aattcttacc
    12601 agttgtatca actgtaaaat tagataaagg tgcttacatc gaatgggata aattgccttc
    12661 aggtggtgaa ggaatcaaga gtgcatttga acaagttaat aatggcttag ccctttctcc
    12721 tgaaaaggta acaacattac aagacaactt agaccaattg attcctggtt tagctgcatt
    12781 aggattaaca ttgttatgta tgtggttgct taagaaaaag gtttctccaa tcgttatcat
    12841 ccttggttta ttcgtagtcg gtgttgttgg acacgtcatc ggacttttat aatccataaa
    12901 aatatagctc gggcgttgtc ccgagctttt tttatatagt atactgttca taaacaaata
    12961 tctggaggga gaaaatggtt caatcaatca atacaaaagt agacttggta attgatgcta
    13021 cttcgtttac gggtctgaca gattacgggc aaatcatgat aggtaataat ggttttgagt
    13081 tttaccatgc gcgagataga cgcaaatata tccaaattcc atgggaagaa gtggattatg
    13141 tactagcatc agtcatgcta aagggaaaat ggattcctcg atatgccatt caaacgaaaa
    13201 aaaatggtac atacacattt tcttccaaag aacctaaaaa agtgcttcgt gcgatcagag
    13261 aatatgtaga tgaagataga atggtacaat cattgagttt ctttgatgta gtgaaacgtg
    13321 gtattcgtag tatgtttaaa cgcaagtaaa aaataaagaa aacttctaag aggaggtttt
    13381 ctttattttt ttactttgca gaagaaaata tgaaatagca aaaccaatta tttgtcccct
    13441 ttaaatccca tatgctaaaa ttgattagca aagaaggagg ttatattatg agttggttat
    13501 ggacattgat tgttggagcc gtgatcggag caattgcagg tgcgatcacc agtaaaggaa
    13561 attcgatggg ctgtgtaacg aatatcattg ctggactagt cggttcaagt attggacaag
    13621 cgattttcgg cgaatgggga ccaaaagctg caggtatggc acttattcca tctattttag
    13681 gcgcagtgat tttggtagca gttgtctcat ttttctttgg aaaacgatca taagttttta
    13741 caaaatagta aactaaaagg agttgcagcg agactgtgac tcctttttgg ctttatttat
    13801 ttaaaataaa aagaagtttt ccggaaattt ttatttaact gtcgtgtacg aagaaaagtt
    13861 tgctataatg gaaaaaagcg tgcaaagaaa gagggagttt cattatgatg ctacgtgtag
    13921 aaaaattgcg caaaaaaatg caagaagaaa atttggattc atttttggtt accagtccgt
    13981 acaatcttcg ttacttaacg aactttacag gtacgacagg attagctgtt attactttgg
    14041 aaaaagcatt ttttattact gattttcgtt ataccgagca ggcggcagcg caggctcaag
    14101 ggtttgaaat tattaaaaat gtcggaccta tttttgatga ggtagcaaac ttagtacgca
    14161 aagaagaact gcactcatta gcttttgagg aaacaacagt ctcattttta gaatattcgg
    14221 ttttagaaga aatcatcgat gcggaattag tacctgtttc acagatgatc gaagaactca
    14281 gagaagtgaa agatgaagaa gaaatcgcta ttatcgaaaa agcatgtcat attgcggata
    14341 tggcatatga tcacatcctg aaaatgatcc aaccgggcat gactgagatt gaagtcgcaa
    14401 atcaactaga cttttatatg cgttctcttg gcgcttcagg tgtttctttt gatacgatcg
    14461 ttgctagcgg attgcgttcg gctatgccgc atggtgtagc aagcaaaaaa atcatcgaac
    14521 aaggtgactt gatcacattg gactttggtt gctattacga agggtacgtt tccgatatga
    14581 cacgtacatt cgctattggt gatcctgggg aaaaattaaa agaaatttat caaatagtct
    14641 tagatgctca attagcagtt attgatgctg caaaaccagg aatgactggt gtccaattag
    14701 atgcagttgc tagagattat atttcaaaac atggttacgg ggaagcattt ggacattcta
    14761 caggacatgg gatcggttta gagatccacg aaggaccaaa cgtttcttct cgtgcagaaa
    14821 agcaatttgt accaggaaat gtgatcactg atgaaccagg gatctactta ccaggaatcg
    14881 gtggagtacg tattgaggat gacttgttga tcacagaaga tggaaatcgt gtgttgactc
    14941 gttcaccaaa agaattgatc atcttatagt ttcatcaata gaagaagtaa gcgtaaaaat
    15001 cagtgttcac agactttgtt atagaaaatc actcgattta atgatacact atagaagaac
    15061 ttttaagctg taagagataa taaagttggg tgttttctcc aattcatagc agtttgcagc
    15121 tgatcggcgg cagttttctt accacgtgat cactatgccg catcatagag aattcggatg
    15181 tatctcgtgc ttaggcaaag ggaggaataa ataaatggca gatgaaaaaa atttgatttt
    15241 aaacgcaaac caagatttag gagagatcgt gatcgctcct gaagtcatcg aagtaattat
    15301 cgggattgca gcttctaaag tagaaggcgt atacggaatg cgcggaactt ttgccagcaa
    15361 tgtcactgaa ttattaggac gtgcagctca cggaaaaggt gtttacttag caaacgagga
    15421 agatggcttg aaagtagatc tttactgtta tctagaatat ggtgtttcag ttcctaaagt
    15481 agcaatggaa atgcaggaac gggtcagcca gcaagtcctt ttcatgacgg atatcgattt
    15541 atctgaagtc aatatccatg tagtagctgt tgttcctgaa aaattagaac aaccaacaat
    15601 cgaagaactt tttgaagacg aagaggaaga gaatgaataa agaattatca agacacgaga
    15661 ttcgggaaat ggcattgcag gctttatttc cattagactt caatgcagac ctaacaaaag
    15721 aagatgcgat tttcaacgca atcgaattag accaccgcga catgatcaac gaagatgaat
    15781 cagagtttgt tcctgtttat cttgatacgc tagttggcgg tgtctgtgca aaaaaagatg
    15841 aattggataa agtgattgaa aaacatttaa aaaacaattg gcacatcaat cggatctcaa
    15901 aaatggattt ggtgatcttg cgtatcgcga tttttgaaat gacttatgtg acagatgtac
    15961 cagcggctgt tgctttaaat gaagcaatcg agttggcaaa aacatttagt gatgaccgtt
    16021 cacgtaaatt cgtcaacggt gttttatcca atgtattgaa agaattagaa gctggggcgt
    16081 aagtcctagc ttttttctat gaatcatcat aaacctgtta taaatcggga aaattctatg
    16141 gtagaatgaa aacacaaaaa atatctggga gtggttagat ggcagaattg attaacggaa
    16201 aagaattagc tgaaaaaatg caggcagaga ttgctgaaaa aatccaaaaa ttgaaagata
    16261 atggtatcca tcccggatta gtcgttttat tggtagggga aaatccagcc agccaaatct
    16321 atgtaagaaa taaagaacgt gtagcaaaag aaatcggaat ccattcattg gtagagcgtt
    16381 atcctgagac gatttctgaa gacgaactac ttgcagaaat tgaaaaatac aatcaagatc
    16441 ctagttttca tggtatattg gtccaattac cattgccaaa gcatatagat gaagaaaaga
    16501 tcctgttagc tatccgccct gaaaaagacg tagacggttt tcatccgatg aatttaggca
    16561 gactccttgc gggtaaaccg gacaagattc cttgtactcc atacggcatc atgaaaatgt
    16621 ttgaagctta ttctatcgat ccggcgggta aacgagcagt agttatcgga cgaagcaata
    16681 tcgtcggtaa accgatggct cagctactct tgatggcaga tgcaactgtt actgttgctc
    16741 attctaaaac cgaaaatctg gctgaactgg cacaagaagc agatatttta gtcgtagcaa
    16801 taggacgtgg acattttgtc acaaaagaat tcgtaaaacc aggtgctgta gtgatagatg
    16861 tagggatgaa tcgtgatgct aacggtaagc tgatcggtga tgtgaaattt gatgaagtgg
    16921 agcctattgc aggctggatc actcctgtgc caaagggtgt agggccaatg actatcacga
    16981 tgctgatgta tcaaaccgta aaatctgccg aagcttttgg agcaggtgaa tagatggcac
    17041 aagaatattt aactgtcact acattaacga agtatttgaa aagaaaattt gatgccgatc
    17101 cttatttaga acgcgtatat ttaacaggcg aaatatcgaa tttccgttta cgtgccaatg
    17161 cccatcaata tttcagctta aaagatgacc atgcaaaaat atctgctatt atgttcaaag
    17221 gagcattcca aaaattacga ttccagccaa aagaaggaat gaaagtcctt gtcgttgggc
    17281 gtatttcatt atatgaagcg ggaggttctt atcaaatcta tgtagagcac atggagcctg
    17341 atggaatagg tgctttatat caagaattgg aagaacgaaa agaaaagctg atgaaggaag
    17401 gcctttttca agggcctaaa aaaatgctgc cacgtttccc aaagcgaatc gcggtcctta
    17461 ccagtccaag cggagcggtt atccgcgata tcatcacgac ggtgaaaaga agatatccaa
    17521 ttgtccaatt ggttttgttc ccaacagttg tacaaggaaa ccaagcagcg gacaatgttg
    17581 tcaggaatat ccagagagta gaagagatgg gcaatttcga tacaatgatc atcggtcgtg
    17641 ggggtggttc gattgaggat ttatggccgt ttaatgaaga gcgggttgct cgggcgattt
    17701 atcaagctaa tacgccggtg atttcatctg taggacatga aacagatacc acgatcgctg
    17761 atttagtagc agatgttcgg gctgccactc caacagcagc tgcagaatta gctgttcctg
    17821 ttttgtcaga agagttgatg cgcattcaag aacggcaagc tagattgcag caggcgtttg
    17881 tacgccagat ccagcgcaaa caagaacggt tcgagcgtgc tcgacgttcc tatgtatttc
    17941 ggcagcctga aagattgtat gaaggtcaga ccatcaaatt agaccagttg aaacagcgat
    18001 tatatcaagc tacacagcaa atttatcatg cgaaagaaaa acaaacgatc gcattaagcc
    18061 accaattgga acaagtggct ccgctttatc gagttaaagc agctcgccaa gaaactgctt
    18121 atctagaaaa acgcttagaa gagaaaatcc aacaatatat gaaacaacag cagcagcgtt
    18181 ttgctcaagc tgttcaatct cttgatttgt tgagtccatt gaaaatcatg ggaagaggct
    18241 acagctatac gacgttaaat gatcaagtag taaaaagtgt caccgagatt catcaagggg
    18301 atttgctgac gatccattat caagatggaa tcgttaaagg acaagtacaa gagatagaca
    18361 aaggagaata aaaatatgga aaatccaact tttgaagagt cactgcaaga attagaaaaa
    18421 attgtcatgc agttagaaca aggagacgtt ccattagaat cagctctaga ttcgtttaaa
    18481 cgaggaatgg aattaagcaa acattgccaa gatacattag ctaaagcaga agagaccttg
    18541 acgaaaatga tgacagaatc aaatgaagaa gttgattttg acggaaacga ggaaagctga
    18601 gatgagatta actgactttt cagctattca tctacctgct gtagaaaaag aaatcttgtc
    18661 ttttttagat gagcatacaa cggatagagg actctatgat gcgatgagtt attcagtgaa
    18721 agcaggcggg aaaagaatac gtcccttatt acttttaacg gccgttgctt cctttgatga
    18781 gcctattgat gttcccgtct atcaagtagc agctgcttta gaaatggtgc atacgtattc
    18841 cttgatccac gatgatttgc ctgcgatgga caatgatgat cttcgtagag gaaaacctac
    18901 caatcataaa gtattcggcg aagcattagc tatattggcc ggagatggtt tgcttacagg
    18961 agcattccag ctaatcagta tggcacactt aggtaattct cctaagcttc tgctgcttca
    19021 gcaattagct gtttgtgcgg gatcacaagg tatggttgct gggcaagctg cagatatcga
    19081 gggcgaatcg aaaaagcttt ctttagaaga attagcattt attcatgaaa gaaagacagg
    19141 gcatttgatt cgttatgcct tgctggctgg tggcatatta gcgaagcaac ctgaagaaat
    19201 cttattgcta cttcaacgat tagccgaaca tttaggttta gcttttcaga taagagatga
    19261 tctactcgat gtgattggta caacgaaaac attagggaaa acagctggta aagatgaacg
    19321 tatggaaaaa aatacttacc cgcgtttatt aggcctagaa aagacacgtg aggcgttgga
    19381 gattgagctg ttatccgcca acaagatcat agacaaatta gaagaggacg ttttttcttt
    19441 tgatggcggg ctgatgcgcc agatgatcaa gcaatttgat gtggagaaat gagggaaaga
    19501 atggaaaaag aacgcgtaga tgtattagcg gtcaaacaag gattatttga aacaagagaa
    19561 caagccaaac gttcagtgat ggctggtttg atttacaacg aaaaaaacga gcggtttgat
    19621 aaaccgggag aaaaaattcc agtgagtagt gaattaagag tcaaaggtaa aaaacttcct
    19681 tatgtttctc gagggggatt aaaattagaa aaagcactca aagcatttga tctatctgtt
    19741 gaaggaaaaa cattgttaga tattggtgca tccactggcg gatttactga tgcagctttg
    19801 caaaatggcg caaaaatgag ttatgcttta gacgttggct acaatcagct agcttggaaa
    19861 ataagacaag atcctagagt cgtcgtaatg gaaagagtga attttcgtta tgcaaaaccc
    19921 gaagacttta ccttaggaat tccagaagtt gctgttatcg atgtttcttt tatttcacta
    19981 aaactgatgc tgcctccatt acatgctatt ttaaaagaag agggagaagt gattgcttta
    20041 atcaaacccc aattcgaagc aggcagagaa gctgtaggga aaaacggaat tgttcgtgac
    20101 cctcagaccc atcaaaaagt tttagaagac attttgtcat ttgcggcaca aggtggatat
    20161 gatgtgctgg aattaagtta ttctccgatc acggggggag aaggaaatat cgagttcttg
    20221 gctcacttga ggaaagtacc cgaaagcgga acaatcaatt cagctattaa tatggcagaa
    20281 gtggtttcaa atgcacacga acagtttgac cataaataaa ttgtaactgt gaaaatcaag
    20341 tgagaagtat gcggattctt ctttattata ccaaggaaat aagctatcat tgattaagaa
    20401 gaatatgcta aggagcgaag acgtaatgag aaaaaaagat cggcatcgtt tgattactcg
    20461 attgttgagt gagcaagata tccgaaagca ggaagaattt gttgaaatcc tgaaaagcaa
    20521 aggaatatct gttactcaag ctacgatttc cagagatata aaggaactga aactgatcaa
    20581 ggtaccggct tcagatggcg gctaccgtta tagcatacca gctgaaacaa acgaagatgt
    20641 aaatactaaa ttggaaaaac tattaaaaga cgctttcgtg gcagttgacc aaatggaaaa
    20701 atttgttatt ttgaaaacat tgccgggaaa tgcttcggca gcagcaaatt tgattgataa
    20761 acgttacaag aaagaattat tttcgattat caatgatgac gataatgttt tgatgattac
    20821 acgaactgaa gaccaggcag ttttattgaa gaaagacttc ctgcattacc tttaagttga
    20881 ggagcgaatg tgatgctaca agaaatcagt attactaact ttgcgattat tccggaattg
    20941 cggctttctt ttcacgaagg aatgaccgcg ctgaccggag agactggtgc gggtaaatcc
    21001 attattatcg atgctttagg tctgttagca ggaggaagag gttcaagcga ttatatacgt
    21061 caaggagcag aaaaatgtgt gcttgaaggg ttgtttgaac taccaaaaca agaaggattc
    21121 tctgaactga tggttgaatt agggatcgaa acagatgagg acaatctgat tgttcgacgg
    21181 gatatgtcct taactggaaa aaatgtttgt cgagtgaatg gtcatattat cacattagct
    21241 aatctgagaa gaatcggcag ttatttagta gatatccaag gacaaaacga acatcaggaa
    21301 ctgctccagc ctgaatcaca tcttgcttta ttggatcgtt ttggagatgt agctttccaa
    21361 cagaagaaaa agagttatca gcaagaatat atctcttatc gtgagctgga aaaaagggta
    21421 cgaaaagtgc agcaaaatga aaaatcctat gttcagagaa tagatatgct ccatttccag
    21481 caagaagaaa tagctgctgc tgatttgcaa gtcggagaag aagagaaatt aaaagaagaa
    21541 agagaaaaac ttagcaatta tcaaaaaatt gctgatggat tagctgcagg ttacggagca
    21601 ctgacgaata atgaacaaaa cagtgtagat ggtgttggct tggccgtttc agaactgcaa
    21661 gggatcgccc atctagatgt ggagtatgaa gcaatttatg aaaatatcca aagtgcttac
    21721 tatttgctgc aagatgcaat cggcgatatg agtcgtcaga ttgatttatt ggagttagat
    21781 gagagtcgtt tagaagaagt tacgcaacgt ttagaattaa tccgacagtt gaaacgtaaa
    21841 tacggcgaat ccattgagtc aatattggct tactatgacg aaatcaccga agagctagct
    21901 tcttctgatt tttcagaagg tcaattggac aaaatgaaag aggaactaga gcaaaaagag
    21961 cttctacttc agcaacaagc cgcagatctt catgaagcgc gtaaaaaaat cgcaaaagaa
    22021 ttggaaaaat ccattctgca tgaattgaaa agtctgtata tggaaaacac tgagtttgag
    22081 gtacgtttct taaaagaaga aaatagacaa ttgaatcatg atggatttga ccaaatcgaa
    22141 ttttacatta cgaccaatcc aggagagcca ctgaagcctt tggtgaaagt cgcttctggt
    22201 ggcgaactgt ctcggatgtt gcttgctttg aaaactattt tttcttctga gcaaggtgtg
    22261 acaagtatta tttttgatga agtggataca ggtgtgagcg gaagagttgc acaggccatc
    22321 gcagataaaa tttctcaaat atcgaaatac tctcaagttt tgtgcatcac tcatctgcct
    22381 caagttgctg cagtcgccga ctatcaatat tatattgtga aagcagtgat tgatggacgt
    22441 acgcaaactt ctgtttcaga gttgaaaaca aaagaaagag aagaagaaat tgcacgtatg
    22501 ctggctggaa gtgagattac aaaactcaca ctcgaacatg caaaagaact attgaaactc
    22561 gcaaaaagga cctgagccac ctcgcagtgg ttcaggtcct tttcagcagc tgatagctat
    22621 attttatttt tacatgacac tcattacagc agtcaataca acagatccaa tcgttaaaat
    22681 cagcattgtc caaatgacaa attttgttac tttagcaaaa gtacttgttt gttttttttc
    22741 acgcattgtt ttcaacctcg tcattaatta gttgattcaa gtgtacaatc ttttttccat
    22801 ttccacaaga gggctttttt ataaaaactt cccttttttt aaataatagg tag
//
LOCUS       DAZAID010000043        22815 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000043 DAZAID010000000
VERSION     DAZAID010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.043"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1252)
                     /locus_tag="ACG9BE_001530"
     CDS             complement(44..1252)
                     /locus_tag="ACG9BE_001530"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFN3032275.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(1364..1696)
                     /locus_tag="ACG9BE_001531"
     CDS             complement(1364..1696)
                     /locus_tag="ACG9BE_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFN3032276.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1844..2719
                     /locus_tag="ACG9BE_001532"
     CDS             1844..2719
                     /locus_tag="ACG9BE_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN3032277.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(2801..3382)
                     /locus_tag="ACG9BE_001533"
     CDS             complement(2801..3382)
                     /locus_tag="ACG9BE_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFN3032278.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(3388..4647)
                     /locus_tag="ACG9BE_001534"
     CDS             complement(3388..4647)
                     /locus_tag="ACG9BE_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN3032279.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(4649..5695)
                     /locus_tag="ACG9BE_001535"
     CDS             complement(4649..5695)
                     /locus_tag="ACG9BE_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032280.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(5930..6205)
                     /locus_tag="ACG9BE_001536"
     CDS             complement(5930..6205)
                     /locus_tag="ACG9BE_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFN3032281.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(6448..7758)
                     /gene="der"
                     /locus_tag="ACG9BE_001537"
     CDS             complement(6448..7758)
                     /gene="der"
                     /locus_tag="ACG9BE_001537"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFN3032282.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(7940..9169)
                     /gene="rpsA"
                     /locus_tag="ACG9BE_001538"
     CDS             complement(7940..9169)
                     /gene="rpsA"
                     /locus_tag="ACG9BE_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFN3032283.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9304..9984)
                     /gene="cmk"
                     /locus_tag="ACG9BE_001539"
     CDS             complement(9304..9984)
                     /gene="cmk"
                     /locus_tag="ACG9BE_001539"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFN3032284.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10033..10668)
                     /locus_tag="ACG9BE_001540"
     CDS             complement(10033..10668)
                     /locus_tag="ACG9BE_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFN3032285.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(10734..12137)
                     /locus_tag="ACG9BE_001541"
     CDS             complement(10734..12137)
                     /locus_tag="ACG9BE_001541"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFN3032286.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(12124..13155)
                     /locus_tag="ACG9BE_001542"
     CDS             complement(12124..13155)
                     /locus_tag="ACG9BE_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3032287.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13186..13407
                     /locus_tag="ACG9BE_001543"
     CDS             13186..13407
                     /locus_tag="ACG9BE_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFN3032288.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(13572..14309)
                     /locus_tag="ACG9BE_001544"
     CDS             complement(13572..14309)
                     /locus_tag="ACG9BE_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFN3032289.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(14462..15802)
                     /locus_tag="ACG9BE_001545"
     CDS             complement(14462..15802)
                     /locus_tag="ACG9BE_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN3032290.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16039..17562)
                     /locus_tag="ACG9BE_001546"
     CDS             complement(16039..17562)
                     /locus_tag="ACG9BE_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFN3032291.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(17940..18551)
                     /locus_tag="ACG9BE_001547"
     CDS             complement(17940..18551)
                     /locus_tag="ACG9BE_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN3032292.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(18674..18799)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(18881..19597)
                     /locus_tag="ACG9BE_001548"
     CDS             complement(18881..19597)
                     /locus_tag="ACG9BE_001548"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFN3032293.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(19603..20172)
                     /gene="scpB"
                     /locus_tag="ACG9BE_001549"
     CDS             complement(19603..20172)
                     /gene="scpB"
                     /locus_tag="ACG9BE_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFN3032294.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20156..20926)
                     /locus_tag="ACG9BE_001550"
     CDS             complement(20156..20926)
                     /locus_tag="ACG9BE_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFN3032295.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(20923..21294)
                     /locus_tag="ACG9BE_001551"
     CDS             complement(20923..21294)
                     /locus_tag="ACG9BE_001551"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3032296.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21313..22200)
                     /gene="xerD"
                     /locus_tag="ACG9BE_001552"
     CDS             complement(21313..22200)
                     /gene="xerD"
                     /locus_tag="ACG9BE_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFN3032297.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(22296..22769)
                     /locus_tag="ACG9BE_001553"
     CDS             complement(22296..22769)
                     /locus_tag="ACG9BE_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN3032298.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
BASE COUNT         6419 a         4885 c         3855 g         7656 t
ORIGIN      
        1 tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc aatttttctt
       61 tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct acgacggctt
      121 tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca gtttcttcta
      181 gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa cgatcatagg
      241 attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat tcttctactt
      301 gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct ccagtttctc
      361 gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta gaacacttcc
      421 acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg atcaataatg
      481 cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa cgtaaaagtg
      541 cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca ataaatgcat
      601 ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca tttatacgtt
      661 ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag gtttcttcct
      721 ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt aaggcatctt
      781 catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct agatcttgca
      841 gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca ttgatcgtaa
      901 aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga tctggtctac
      961 ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt tcatcatttt
     1021 ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga aaaagctgtt
     1081 tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc tggttcagca
     1141 agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc tctaattttt
     1201 tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc atttttccaa
     1261 cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga ctgtttgaca
     1321 agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg tccttacgct
     1381 caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa acttcagtta
     1441 agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca ccaagttctt
     1501 ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa gtgtggttga
     1561 cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct aatggagaga
     1621 aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata ctgtataaag
     1681 aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt aacgcaaatc
     1741 ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag gaatttctaa
     1801 atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca aacgaaaaac
     1861 agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg gtcttgttta
     1921 cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct tgatcttaag
     1981 agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc cattgatttt
     2041 actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag gtacactttc
     2101 tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt tacagcatga
     2161 tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg ggatcgtcta
     2221 ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag aacaaaatta
     2281 cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac ttgcttcatt
     2341 aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg ttttcagtcg
     2401 tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg tcgtctccaa
     2461 caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg tgactttcct
     2521 ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg tcgttagctc
     2581 tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag ctttcctttc
     2641 aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc cacagacaag
     2701 attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag attccacatc
     2761 cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta tgttcactta
     2821 cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca tcagataatt
     2881 tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca taaagcattt
     2941 ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca gtatcttctt
     3001 cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat agatattctc
     3061 ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc caattcaaac
     3121 ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa aacaatttca
     3181 tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca aaattcgccc
     3241 cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa ttcaagatcc
     3301 aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa aaattcttct
     3361 tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta ataatgacag
     3421 tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt gcgctctttt
     3481 cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg gttcatgtga
     3541 tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt ctaattcatt
     3601 ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca tctgattgat
     3661 cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta aagtaagtaa
     3721 tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt ctgctcgctg
     3781 tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga cttggtaagg
     3841 attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt cttcttcttg
     3901 aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt ctcgaagttc
     3961 ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa aagctagctg
     4021 gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta cgatagctgc
     4081 ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg agattcctga
     4141 aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct ctttgaaacg
     4201 atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat caggaagcaa
     4261 cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa cttggtacaa
     4321 atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca ctttttccag
     4381 gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta acgggatgtt
     4441 cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt ttgcttcgat
     4501 caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg ctgggtcttc
     4561 ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg ataaagcttg
     4621 caacattttt tcgctatttg tattcatttc aattccctct tatctcactg acgatttgtt
     4681 gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat ccgtctatta
     4741 tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa agaacttttt
     4801 ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca agcaagtcca
     4861 gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga ttccacacga
     4921 aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg ccattttcgg
     4981 tcagataaaa agcttgttca aacagtttac cataatttga caggttttct gcctctaatt
     5041 gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt aattgctttt
     5101 tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc attccgcaac
     5161 agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg attgtttgat
     5221 cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt ggtagctgca
     5281 aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca gggatcatcc
     5341 atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc atgatccctt
     5401 catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg acgaaatcaa
     5461 gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata taccagtcat
     5521 tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca taatagcgct
     5581 gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga ctgactagcg
     5641 tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt tccatacgca
     5701 tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa agataagagc
     5761 aaagctcacc tcataacttg caaataaata aaaattaaat aaaatacatg tgttttctat
     5821 gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc taggaaataa
     5881 aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct tatttaacag
     5941 cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga atttcgattt
     6001 cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact tcaaagttac
     6061 caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata gttgaaaata
     6121 cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa gcaacttttt
     6181 cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga gatatgatag
     6241 aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa atattgatta
     6301 acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg tttttaccaa
     6361 ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt tttcttttga
     6421 aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa ttcggatgga
     6481 cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt cataagaaaa
     6541 atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga cagcgacttg
     6601 tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat tgattgctac
     6661 agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct ggttcatact
     6721 tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg cagaaacaaa
     6781 aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt cgaaatcacg
     6841 cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa taattccacg
     6901 accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc cttcttcagc
     6961 attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac gcatcacgct
     7021 gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg tatctatcat
     7081 taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc gggtcgttcc
     7141 ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca aagaggattt
     7201 accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct cttcttcagt
     7261 ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta gtccatggct
     7321 tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga tatcgtttct
     7381 catttctgga ttatctactt tattgactgc taaaatcact ggtttattac ttctgtataa
     7441 aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga ctacacaaat
     7501 gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat ccataaaagg
     7561 ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac ctaaccattc
     7621 accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa tcgagatacg
     7681 ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc caacgattgc
     7741 aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac ttagtttaac
     7801 gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt ttattcctta
     7861 ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc tttcatctca
     7921 gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct tctacttctt
     7981 ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct tctggtaatt
     8041 cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct aaagctttga
     8101 tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact ttcacttcgt
     8161 caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt tgagagatat
     8221 ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt gtcaaacgtt
     8281 taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct gaccaaggtc
     8341 caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg atagacaaca
     8401 ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct acatgactat
     8461 gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca aatgcgccaa
     8521 aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata gttgaaagaa
     8581 tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat aagatcaaac
     8641 gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct ttatagtctg
     8701 aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca cgtacaccga
     8761 cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg accttgccct
     8821 cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg cgttttgaaa
     8881 gtagatagct gccgttttct ttatctttgc cgatcgtact gataacgact aaatctaata
     8941 catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat aattcttttg
     9001 ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt ttgtcttcaa
     9061 tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg cttttcattg
     9121 cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata acacttgtcc
     9181 tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga ttattcaaaa
     9241 aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat tttttttctt
     9301 aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg caacgacttc
     9361 tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct tcagcggaga
     9421 gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca gtgtttcaag
     9481 atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac gctcacttac
     9541 gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc cgatgtctct
     9601 tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa ctaattcttc
     9661 tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg gttgacggat
     9721 ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt gttgcgaaaa
     9781 agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa aagatacatt
     9841 gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg tataaatata
     9901 attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag caggcccgtc
     9961 gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa aagccgcaaa
    10021 gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa agttgtttgg
    10081 atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct cagccacttg
    10141 ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg atgaagaagg
    10201 tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct cacttgagga
    10261 agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat cttgagacac
    10321 agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg actcctcttt
    10381 tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag ttgctgctgt
    10441 tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa tagcgataca
    10501 taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct tttgttgtct
    10561 ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt cccatggttc
    10621 ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg ttttacctcc
    10681 taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa aaatcaatgt
    10741 tccatgtttt ttttaaataa atttagtaaa atcgttcccc aatgaagttg ttcagtctta
    10801 gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc acaacaattc
    10861 tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct gcggcagttt
    10921 tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt ttcctgacgt
    10981 ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc catccatttt
    11041 ttttgcagct catcaaaggg agcttgctct gtggcatatt ccaaataaag ctcaaaactt
    11101 tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc cccactttga
    11161 taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat ttcttgtaca
    11221 tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc agatttatcg
    11281 atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt tacaaactgc
    11341 tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc aactgtaaaa
    11401 cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca ataaatgatt
    11461 gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg ctgcgttttc
    11521 cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt tggtggtcga
    11581 tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc cgttagtgcc
    11641 agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata ttctggacgg
    11701 aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa cgcaatattt
    11761 tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact caaaaacaaa
    11821 aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga aagtaaacta
    11881 ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg atcttccatt
    11941 agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc cgtcaattga
    12001 tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc tccatcaagc
    12061 aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa atattttttt
    12121 aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt gatacaaacg
    12181 aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc actctcgcac
    12241 ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc actcgtatgg
    12301 aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc ctctttttaa
    12361 atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct gcttggtgat
    12421 catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt gccaaagcga
    12481 atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt gttgcagata
    12541 aaccgctgac tcatcattgt tttttgccag ctgataagga agcagccctg ttcgctcatt
    12601 tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg aaaacagctg
    12661 caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc tttcactaag
    12721 ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat agtcttgaat
    12781 acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc aactagtttc
    12841 ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat gttcggacaa
    12901 catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga tccaaccttg
    12961 ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg gaaaactgtt
    13021 atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg ttcgttttcc
    13081 cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa atagcgataa
    13141 aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc actactatgt
    13201 aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc gccagatatt
    13261 ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc tcatcaacag
    13321 ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg tccagttcga
    13381 gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg tacactaagt
    13441 tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat cccgacttat
    13501 tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg accctatagg
    13561 acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac gatcacagtt
    13621 tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc atagctgcca
    13681 aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg taaatactgc
    13741 ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt gatcgataaa
    13801 acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc catgaagaat
    13861 acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc cattacgcca
    13921 cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt gaccttatcc
    13981 gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc gactgaaaag
    14041 ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac gagagcacca
    14101 ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc aaaaaataag
    14161 taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga tggttcgtac
    14221 gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat gatgactaag
    14281 ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct gagtgacccc
    14341 tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc aaactttcca
    14401 gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg tatgtttatt
    14461 tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct ttatacatac
    14521 tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca gggaacatcg
    14581 caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct gatacaaacg
    14641 gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc actaactcac
    14701 tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc aacccatatt
    14761 tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta acagggattc
    14821 tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca aagttcatcg
    14881 ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca gttgcatttt
    14941 cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct gaaagagcaa
    15001 ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg atacttttac
    15061 caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt ttcaatgctt
    15121 cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca acattcaacg
    15181 ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa gcttgcaata
    15241 agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct tgcgtaattc
    15301 cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca tctgtaccaa
    15361 agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct ttgacggcat
    15421 acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact aaccagccgt
    15481 cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct gctggaacaa
    15541 cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct gaataaccgt
    15601 aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt tccaatactt
    15661 tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa tctgcttcat
    15721 ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt tcatgatgtt
    15781 gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag ggtcatgtca
    15841 aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg ctccatcagc
    15901 aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg agctattgac
    15961 ttatccacgt gcttatcgat agccgtttat cggtttcttc attcttatga tagaccttgt
    16021 acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac agatggctac
    16081 aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat cgaaacgagc
    16141 aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta ctaaaccaga
    16201 tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt cacccatagg
    16261 tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca agaatgacat
    16321 agggatatag aaaataaatg taataatgat gaatacactt gaagataatg aacttaatcc
    16381 ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa ccacttggat
    16441 accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac cagctaaaaa
    16501 ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga tggacattac
    16561 taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca tgcttttacc
    16621 tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt caaaaatcgt
    16681 gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac caaaagtaat
    16741 aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac gaccgattgc
    16801 ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg attttgttgg
    16861 atctttttcg atctttgatg cataacgata cacatataca atagaaatag ctaacaaaat
    16921 aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa cagcttgtga
    16981 agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt gagaccctac
    17041 taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga ctgggatcag
    17101 taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc ctaatgcaaa
    17161 taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct tcacaactga
    17221 agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc caccaacaac
    17281 caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct cccccgctgg
    17341 tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt gaggattttg
    17401 ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac tatagtgccc
    17461 agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata agaccgtata
    17521 cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct tcctttcttt
    17581 tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt atcagaacaa
    17641 aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc cctattccct
    17701 cctctttttt caggttttta aagctttctt ctttttcatt gtagcgtttc ctttttttta
    17761 taaacaaaca ttttcgtctt ttaaaaggag aagaatttca catttgcaaa gaattgattg
    17821 atttttatgc ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt tacattattg
    17881 catgtcttgt atttttttcc aaaaaaaaag accaagaaat ttcttgatct ttttgatatt
    17941 tatttgactg ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa ccatggaaga
    18001 agttgcgcat gcagcactaa aaagacagca ctcacgatga atcctttgat caagttgaaa
    18061 ggaacgatcc caatcagtac atagtttgct aaagacatgc ccagcatctg tgttgcgttc
    18121 agccctaaaa acattaagta aagaggcgta ataacgaaat aatttgcgat actcataaaa
    18181 atagttagag cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc tttatgctgt
    18241 ttctttacac taaagaaata ataaatcggg aatgtaaaga tcgttgttga taagaaacta
    18301 gcagtgtctc ctaccaaatt atccggagaa aaacctgtag tcagcagatg taatactgaa
    18361 cgcaagaacg ctgttgcaac tccggccgct ggaccgaata agaacatgct gatcaatacc
    18421 gagatatcac taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg aaaagcaaaa
    18481 tactgtaaaa ctaatgcgat cgcagcaaac atcgctactg caaccatttt ctgtacacgg
    18541 gtgttcttca tttgaaatcc tcctgttagg actcctcttc agcgaacttt ttgacgtttc
    18601 tctccaaatg aaaaaactct attttccagg gaaaatagag ttagagttat ttgatcaagc
    18661 gtcgtcaaat aagctctatc ttctttatcc agactatact gtcggtatcg gaatttcacc
    18721 gattcagcta tttttttgca aaataggtcg tggactataa ccaccggtcg ggaatttcac
    18781 cctgcccctg aagacgaacc ttatataatt tttgtttaca tattcattat acatatctat
    18841 tcaaaaaacg caacatctaa cacttataaa aagtaagtgt tcattttgag tggttcagca
    18901 aactgctgac ttcttttttg ttcagttcac ggtaatcccc aggacgtagg ccttgtaaag
    18961 tcagatctcc gtatttttca cgttttagtt tttgcacagg aagaccaact gcttgaagca
    19021 tatttttcac ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata ctgtggtttg
    19081 tcttaggatc cactgataaa atttggaatt tagctggaga tgtttttcgt ccttcgatct
    19141 ttattccttt tgtcaaaggt gcaagcatcg attttgtagc gatccctttt acctttgcaa
    19201 cgtaaacttt atccacttca tgttttggat gtgtcaggcg ctgagcaaaa tctccatcat
    19261 ttgtcagtag caagatcccc gaagtatcgt aatccagtcg acctacagga tagatacgtt
    19321 cttttaccat cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct gcaacagcag
    19381 aaataactcc tttaggtttg tacaataaat agtaaccgta ttcttcttga taaattggga
    19441 cttcatccac ttccacagtg tcatgtttcc caacttttat ccctaattcc cttacaactt
    19501 gtccgtttac ttttacacgt cctgcaagga tgaattcttc tgcttttcga cgagaagtga
    19561 tgccagcgtg ggcaattact ttctgcaatc tctccattac aattattctc ctttattcat
    19621 tccatcaaag aatagatctg gtgacacgtc ttcttcaagt gaaatttcca tttcttgtgt
    19681 atcaggcagt tcttctaatg atttcaaacc aaaataatct aagaagtagc cagttgttcc
    19741 atataatatc ggccgacctg gtccttctac tctacctttt tcttcaatca gtttatgggc
    19801 agctagacgc tgtaccgctc ctgaaacctg aactcctcga atctgctcga tttcagctct
    19861 tgtgattggc tgtttataag cgatgattgc taaggtttcg acggcggctt gagataatgt
    19921 gtttgatgtc ccttgcgcat atcgcttaag taagggagcc agctcttttt tagtcgttaa
    19981 tacataatgg ctttccgttt caaaaatatg caaagctgag tgcgagtttt gagtgtaaga
    20041 ttcttttaat tctttcacta aacttgtgac ttctgcttcg ctgagattta acagtccgct
    20101 taattcttca ttactgatcc cttcctcgcc aacaataaaa agaagggctt caagttcact
    20161 taatttcgtc atgttcggta tctcctactg ctggataaag cataattgat tcatagtttt
    20221 ctttttgttg cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc ataaaagtag
    20281 tgacaacttc acttttagaa ggtgactcaa aaaaagaatc gaaagtacag ccatgcgcat
    20341 ttctttttat acgctcttcg ataaatacca ttcgttcttc gatcgttgtg tcatcgggcg
    20401 tgatcgtttt ttccactttt tgagaaaatt tcttacgttc taagattttc tgcatggcac
    20461 ggatcaaatc gatttttttg acttgtcctc gcttcaacgg cttttcagca tccgctaacc
    20521 catcgatgtt cataggttct tttgtgtaat aaagacttcg ttcctctgct ttttctgtca
    20581 gtctttcagc tgcatattta tatttacggt attctaaaag ctgagtgacc aattcttctc
    20641 gaggatcttc ttcccataat tcttcatcgt ctgtgacgaa ttcttggaca ggaagcaatg
    20701 tcttgctttt gattgccatc aatgtagcag ccatcactaa gtattctcct gcaacagcta
    20761 gctcgagtgt tttcattgta tggatataat tcatatattg ctccgtgatc gtagcgatcg
    20821 gtatatcata tatatcgatt tccatttgct gaatcagatg aagcagcaga tcgagaggac
    20881 cctcgaatac atctaattta atattgatct ccgtcaactc attcactctt tcttcttttt
    20941 ctttgctagc cattttccaa tgtaaggctc tgtgatttca gttgcacgga tggagacatc
    21001 cgggtacttt tcctgtaatt catctaacat ttgaaagccc atattctcac cgcgataaga
    21061 cggattcaaa gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt aaataccaat
    21121 aatcccttga atgttggttg aaccttctgg cgtaaagaaa aaaagttcat aatcgtcttc
    21181 agattcgtac gtatcaatct ccttcaacaa agaagtttta cctgtcagtt tcttatggaa
    21241 actcaatagt cccatagcaa ttttacggtt ttctttccgg taatgtgtta acatcttctc
    21301 accttcctat gactaagccc ttggaaaatg ctgcttataa acatccgtca ttcttttctt
    21361 cgtgatatgt gtataaatct gagtcgtaga gatatccgcg tgacctaaca attcctgtac
    21421 tgtccgcaaa tcagctccat tttctagcag atgtgtggca aagctatgcc gcagcgtatg
    21481 tggtgttact tctttgtaaa tcccagcctc tcgtactaac tgcttcagat ttttccagat
    21541 tccttgccga gaaagacctt tcccatgatt attgacaaat acatgcattt cagatgcgtc
    21601 ctttacaagt aatggacgtg cttcgtctaa gtagcgttcc agccattgga tcgcataatc
    21661 acctaaagga atgatccgct ctttatctcc ttttccaact gtctgtacca gtccaagaga
    21721 aagatgaaga tctcccaatt tcaatccaat caattcactg acgcgcatcc cagtagcata
    21781 catgacttcc aagattgcac gatctcttat ccctaacgtt tttgtcgtat caggtgcttc
    21841 aatcagcttt tctacttctg tcagagtcaa tgtacttggt aatttctgtg ctttcttcgg
    21901 cgtatcgata tgttgcatcg gatcatgatc agtgatacgc tcttgccgta aaaactgatg
    21961 gaatcttctg agacttgaga tcatccgagt aacagttgct gtcgcttttt tttcttcatg
    22021 caacttctcc agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg aagcaatttt
    22081 ctgctcctgt agaaaatgca agtactgttc caaatcacgt tcatagcttt gtcgtgtatt
    22141 ggcggacaat ccccgttcaa tattcaagta gtggagataa tcgttgattt gctctttcat
    22201 aataatcccc ctcatgcctc tctatcatat cagataccgc ttcattctga tagtcttttt
    22261 ccataatctg agcaaagaaa aagccgtcta gacactcaga cggactggtt tcttttttct
    22321 ttttcatagc attcctgaca gattccatgg aacgttagcc gatgatcttt tactaaaaaa
    22381 tgataacgtt cttctactac ttgctccact tcaagtaaaa gatcctcttc tacttcctcg
    22441 atcgtgccgc agttcaagca tagtaaatga tgatggaaat gtttggcgcc ttcttttctc
    22501 aaatcataac gggctaatcc atcattaaaa cttactttat cgacaatttt caattctgtc
    22561 aagatttcaa gggttcgata aacagtagca agtccaattt ccggactctt ttttttcact
    22621 aaaaagaaga tttcttctgc agataaatgc tctttctcat tttccaaaag cacaagcaga
    22681 gtagcttctc tttgcggtgt cagcttaaac cccgcttcat gtagctgctg ttttgttttt
    22741 ttcatcgccg aaatagcatt catgaccatt tactcaaccc caacaattta taataattac
    22801 aaataaaaaa agata
//
LOCUS       DAZAID010000044        22799 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000044 DAZAID010000000
VERSION     DAZAID010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.044"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            180..3458
                     /locus_tag="ACG9BE_001554"
     CDS             180..3458
                     /locus_tag="ACG9BE_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFN3032299.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3999..5906)
                     /locus_tag="ACG9BE_001555"
     CDS             complement(3999..5906)
                     /locus_tag="ACG9BE_001555"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFN3032300.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6036..6911
                     /locus_tag="ACG9BE_001556"
     CDS             6036..6911
                     /locus_tag="ACG9BE_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFN3032301.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6991..7965
                     /locus_tag="ACG9BE_001557"
     CDS             6991..7965
                     /locus_tag="ACG9BE_001557"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFN3032302.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8145..9290
                     /locus_tag="ACG9BE_001558"
     CDS             8145..9290
                     /locus_tag="ACG9BE_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFN3032303.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9427..9771
                     /locus_tag="ACG9BE_001559"
     CDS             9427..9771
                     /locus_tag="ACG9BE_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFN3032304.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10042..11328
                     /gene="efmA"
                     /locus_tag="ACG9BE_001560"
     CDS             10042..11328
                     /gene="efmA"
                     /locus_tag="ACG9BE_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFN3032305.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11466..12065)
                     /locus_tag="ACG9BE_001561"
     CDS             complement(11466..12065)
                     /locus_tag="ACG9BE_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN3032306.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12469..13590
                     /gene="mnmA"
                     /locus_tag="ACG9BE_001562"
     CDS             12469..13590
                     /gene="mnmA"
                     /locus_tag="ACG9BE_001562"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFN3032307.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13836..15257
                     /gene="arcD"
                     /locus_tag="ACG9BE_001563"
     CDS             13836..15257
                     /gene="arcD"
                     /locus_tag="ACG9BE_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFN3032308.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15271..16389
                     /locus_tag="ACG9BE_001564"
     CDS             15271..16389
                     /locus_tag="ACG9BE_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFN3032309.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16449..17741)
                     /locus_tag="ACG9BE_001565"
     CDS             complement(16449..17741)
                     /locus_tag="ACG9BE_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFN3032310.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17891..18451
                     /locus_tag="ACG9BE_001566"
     CDS             17891..18451
                     /locus_tag="ACG9BE_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3032311.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18580..18957
                     /locus_tag="ACG9BE_001567"
     CDS             18580..18957
                     /locus_tag="ACG9BE_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFN3032312.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18890..20962
                     /locus_tag="ACG9BE_001568"
     CDS             18890..20962
                     /locus_tag="ACG9BE_001568"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN3032313.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21173..22120)
                     /locus_tag="ACG9BE_001569"
     CDS             complement(21173..22120)
                     /locus_tag="ACG9BE_001569"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN3032314.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22366..>22799
                     /locus_tag="ACG9BE_001570"
     CDS             22366..>22799
                     /locus_tag="ACG9BE_001570"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFN3032315.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         7385 a         4154 c         4817 g         6443 t
ORIGIN      
        1 ttattttaaa aaaacttatt ctttttacgt taataaatct ctttattatc aacaacttaa
       61 gttatatttt ttctttttat aaaatacact tattataatt ttgtttttta ttgacagtaa
      121 aaataaatta ggtaatactt taagtaaacg ctttctaatt tgttacggga ggaatacact
      181 tgttaaaaaa acgtataaaa tacagtgcgc taatgatgaa tctccttatg ctcagtaccc
      241 cggttttaat ttctgtcgtt gaagttgctg ctaacgaaca acagtctgtg aacgaagaaa
      301 atgcacacgt gaacattgaa gggatgtcaa caatggagga aataccaaga actagtgaag
      361 atatggatac ggaagaagga aatgatccta tgatgaaaga atcagaaaat gaaaagacaa
      421 aaaagcaatc tgaagaagca aatgaagagt ggaaaggggt tgttttcgga gaaagcacaa
      481 gtgctagtag gtatgccatt gaaccattag aagacggcgt gcggctctct tccacgaata
      541 acggcggaaa attccagtca agcggatcag atggaatgac ctattattat caagcaatcc
      601 ccaaagacat caactttagg atgcgggcga cgatcgaaat agaatcttgg acctatacga
      661 atgcccaaga aggatttgct ttaatggtaa gagacgcagt accaaaaaat catttgtttg
      721 ggcaggcatt ttattcaaat tcatttgcca tactaggcag tcggatcgaa tatgtctggg
      781 actcggatag acaagaagtg gtcaacacga gcggcagcaa atatacgatg cgacttggat
      841 tggggacacg ggcaatcaca ggaatatcat ctgaagatcc gcaagctgcg ccagctcctg
      901 ggagtgtctc agtggaaaca acacctttag agacttcagc agggttcttg gaaaaagaaa
      961 caggttctta taatatattc ggaaacggtc atgggaatct gtttccggcc acgaactcga
     1021 ttactaaact ggacgtagag atgaaaaaaa cgaatactgg tttagaggca tattattacg
     1081 atcctgaaac aggagaagaa atggcttctg acatcatgta tgactgggaa aaattatacg
     1141 cttcagatga atcggttatt tatgcaggat ttgctgcagc aaggaatatg acgatcaagg
     1201 tagaaggaat cgaagttgct tactcagatc ctgcgacaga tcctcctggg caagaaagac
     1261 ctactcgctt gattgatccg atttacactg tgacctctag taatcattca ggaaaccaag
     1321 atcatactat gtcctttaga gcaaatgctg acggattatt gacaattaaa aataaagcaa
     1381 caggagaggt gcttaagaac gcaaaaaact tggctatcac tgcgaatcgg gaatttgatt
     1441 atcagacaaa actgatggaa ggaacgaacg aatttacttt cagttttacg ccagataaaa
     1501 actatccgcc agaagaatac gtagagatga ctagttatga aacaaaagag attcttcata
     1561 cagtggacta ccgtaaacct aaatattcga cagtttatgt aacaccagaa gggtcagatg
     1621 caggaaacgg aagcagacaa aatccattat ctctgacaca agcattgaga tacgcatatg
     1681 ctgggcagac aatcgtcatg gcgcctggaa cttattcctt cgaaagagga ttgacgattc
     1741 caaaaggcgt taacgggaca agagaaaacc cgattcagtt gattgcagag aaaaatccag
     1801 aaaacaagcg tcctgtactt gattttaaag gaactggaaa tgggatgaca ctgttcagtc
     1861 actactgggg tttacgaggg tttgacatta caaatagtgc agctatgcaa aaaggattac
     1921 agatttctgg aaatcataat ttgatcgaag agattcatgc ttatcggaat ggaaatacag
     1981 gaatccaaat cagcggtagc tcaaatgatc ctttcgaagc atggcccgcg cataacttag
     2041 tcaagaactg tacatctttt gaaaatgccg atccaggatt tgaagatgcc gatggatttg
     2101 cggccaaact gacaatagga gaaggaaatg tatttgacgg ttgtatcgcg tatcataatg
     2161 cagatgacgg ttgggacctt tttgctaaaa atgaaacagg ttcgatcgga aaagtaatca
     2221 tcaaaaactc agttgcttat cgaaacggct gggtaccagg aattgaggga gaaggtaacg
     2281 gtaatggatt caaaatgggc ggttcttctt taacgggccc tcatgaattg atcaatagtt
     2341 tgtctttcga aaatcttgcg aaaggaatcg attccaatag cggaacagat attatcgtca
     2401 atagcagtac aagttttaat aacggttcat ataatgtggc tctttatacc tcttcagcag
     2461 gaaacacaga ctaccatgct tctggtatct tatctttccg aacagagaat cttgagatga
     2521 gagagcagat ccgcccattg aatcaaaacg aagatcaaat ctaccactcg ttgaactact
     2581 attggaatga acgagagaaa caatcagaaa atactcttgg acataccata tcaaaagatt
     2641 ggatcgagtc tctaaataca tgttcgaaag aaggtcagca gcctgttacg cgctatgaaa
     2701 atggaagtat ccatgttcat ggactgatgc agattaaacc ggggaatcgt caaacagaaa
     2761 aggaaagagt ggatggtctt ccattatctg taggtgctat ttttgacgga gaaacctcac
     2821 cgtcttcgga atatccagag tatgcaatcg tacaagaacc aaatcagaca aaaccaaatg
     2881 aacaaaataa tccgattatt atacaagaag caaatggtgt ggcggagaat gcagaaggaa
     2941 atatcattga actgaagcct tttacagttt ttgtgtcaac tttcacacca aatcaaggaa
     3001 cgcctgtctt tgaagatatg cgttatttcc cagaaaagat ccaaatcgtt gaaacacagt
     3061 caagtgctca attttctaat cataccgcta ttttttcagc accttcaaaa ggagaatata
     3121 cgctagaagt cacttatcgt tcggaaatat ttgatgggga aaaatggtct gctcatacag
     3181 aatctgttac ccagcaaaaa gcgatcactg tgcgagaaac tgccgatgtg cccccgggaa
     3241 atgacaaacc agaaagagaa aatccaggaa atgaatcacc aaatggaaaa ccaaatggaa
     3301 aaccttctca tccggatagg acagcaaaag aaagtgaaaa aaatggcact caaggactcc
     3361 caaaaacggg agaatttaat aacccgttgc tagcattagc tggaggaatt ttgttaatcg
     3421 gtgtagttgt ttatgtcatg aaacagcgaa aaaaataagt ggaatttctc cttttatgat
     3481 gtaaaacaat tgactgagga aagtaaaaat agaaggaaat gagaccaata gatgtctcac
     3541 ttccttctat ctttttatag atgaggttct ccttgctgct tttgattggc tgttcttatt
     3601 acttgatgca ggacagcttt ttcacaatct ctccactaaa cggtgttcaa tcagctgacc
     3661 ctcggtatta gctcaacaaa cggcaaaata tgaggaacta tttttccgtt tttttctcta
     3721 atactcaggt caggacgctg atttcacaac ctctcgataa acggtgttca aaagccagtt
     3781 tctcggtagt aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct
     3841 tactacttga aactaagcag ctttttcaca acctctaata catggtattc cagaagtaac
     3901 ttcttcgaaa aataagccga aatgtcacaa aaatttgaga agcgattttc ccaaattcct
     3961 tcttatttct tgaagctgac cacttctggc acaacctctt accagattcg cactcttttt
     4021 tctggcgcta gatacatagc gtcttgttcc gtgataccaa atgtttcata gaattcgtca
     4081 aggttttttg gttgcacgtt tgctcggagt ttagcagggg catggacatc gatttgcagt
     4141 aaaagttgtt ggtattcttt ttttgctttt gtacgccaaa tcgttgccca attgacaaag
     4201 aattcctcga tagaaagatc tgattccgtt tttgctgctt ccaatgcaca gcttagaccg
     4261 cctgcatcag caatattttc cgaaacggtt aattttccgt ttacttttcc gtcagcaaaa
     4321 ggcagaccat cgaattcttc gatcattgct tgtgctttgt tttgaaattg tgtaagatct
     4381 tcttctgtcc accagttgtt caagttgcca tattcgtcaa ataaagcgcc gttattatca
     4441 aatgcatggg aaatctcatg agcgataaca gctccgattc caccataatt agcagagctt
     4501 gattgttcta gactgtaaaa tggtgcttga aggatcgctg ccggaaagac gatcacattc
     4561 cgaaatggat gataataagc attgaccgtg tcagcactca tttcccattc tgaacgatcc
     4621 actggtttgt tccatttgct gaaacgatct tttaatgtga gtttgctgaa aacaagagta
     4681 tttgaaagca aagtaccacc ctgatctgca ggtactgttt tgaactggtc gaataatgct
     4741 gggatcttat cggggtagcc tacttggata cctaagttat ttaattttat gacggctttt
     4801 tcttttgttg catccccaag ccagtcattt tcttctagac gttttttata aacggcgatc
     4861 atttttttca ccatttgttc tacgtctttt ttagcagctt cgccaaaata tttttttccg
     4921 tagtaatcgc caactacttg atcgaattgt ccagaggcaa ggtaatacgc tgcttttttc
     4981 tgaggcattg cttcgtctgt tccagacaat acgcgagaat agattccgct gacttggcgg
     5041 aattcttctg atagataacc actaagagaa cgtactgttt taatgatcat ccagcttttg
     5101 aataacggaa aatgagcttc tgtcaggatg tcattcaaat gatcgaagta cactggatca
     5161 gttacgatca ctttatctgg agttgtccca atcaaacccg tgacaagttt tttcaagtcc
     5221 agtttatctg tgtatttaat aaatgtatca aaagattgcg gattatacat tttgctgtaa
     5281 tctgcgtttt cttctgcact ttttacatga ggagcaatca atttatcaaa ttgcactgct
     5341 tgttccgtga ttgcttctgc ctgacttttt tctttgccgg caagctctaa taactgaaca
     5401 gccatatcaa agaaaacgcc tattagttgt gcaccgttgg gatggtctga tgcataatac
     5461 gtcttatctg gaaggaataa agaaggcgga taagcaaaga aggcgttcgt ttgcgcattc
     5521 ttcatatctg catctacatc cagattaaat ggcaatggta atgtatccaa cagccattct
     5581 ggaaattgct tattcaattc gtcgaaagac tgcaatgatt caactttttc gattataggt
     5641 tgaagcggtt gagcatgcaa ctcatttctt cggtcataat cgtttgccag ttgataatat
     5701 gcaaggaaat gtttcatttt ttctgaagac actttttctg gatgggcaag cagatcgtct
     5761 gtttctccca tcaaaagttg gtcgatcccg tcaactaaat cttggaaacc gcctgtagct
     5821 ggtttatctg cagggatttt tgctgttttt tgccattctt cattgactgc ttcatagaaa
     5881 tcttgtttca atcgttcttt gttcattcat cgatcgctcc ttttttgttt cgtgtgccca
     5941 ttatcttaca aaatgaagga attaacaaat agaacataaa aaattgaaaa ttcgaacaat
     6001 caaactgatt tgtttttttc aatccttcac ttataatgaa aaaaaggagg cgatcccaac
     6061 tgaatcaagt agtggataaa cctttatatt tcaaagtaga taaaaaaatt aaaaaattag
     6121 ccagcactca gcagctgcag tcaagaaaaa gtaaattgct ttttttagct ctggtttttg
     6181 aagatcaaag ttatgtgatt atcgatcaat caggacatcc ggtagaatat tctccagcaa
     6241 aatatactta tcaagagggt ctttcttgta aaaaatggaa gctgatcaat gaagagccaa
     6301 tcgagttatc tagatggatc aacagaaaag aagatattcc cgttttgatc gaagaaaaaa
     6361 ggtcaggaaa ggaactcgca aattgctggg tgggattgcc agaagaacgt tttttgaggt
     6421 ataagaaatg ggctaccccc tcaggctatc tttgtggtac ttacgcggca gcagttctcc
     6481 ttgcctatta tcaagactat cgtaaggggt ggatgcttcc aaatgagatt cgcaagaaaa
     6541 atacggctga ttcgttggtt ttgacgaagg cattgcgtag ccagatccaa ccactaggac
     6601 ttcctacgat tccttttcag gttagtacag ggatttctag ttttttaaag aagaatggca
     6661 atcatgagcg tgcacgagca actcttctag gttcatggca gcgagctaca aaacgtatac
     6721 gtgaaggcaa acctgtgatg attggtattt tgaaagtact tggcagtaca tatggcaatc
     6781 attgggtgac agcgtacgct tattttgaaa ctgaaacagg agaacgttat tacaaagtac
     6841 acgacaactg gggcgattat cataaagtca ttcctgccag ttggagtaac ggaacagttt
     6901 cacttcctta agaatttgct gaaattaaca gaaaatcttg gaaaaatatg ttaaactatc
     6961 gaagaataga gaagttttgg ggaggactac atgagtaata agtatcagga tgacacgttg
     7021 aagattttta gtttgaatgg caaccgtcca ttagctgaaa aaatcgcgaa agtgttcgga
     7081 acagaattag gtaaaagcgt cgtgaagcaa tttagtgatg gcgagatttc catcaatatc
     7141 gaagaaagta ttcgagggga tcatgtgtat atcgttcaat caacgaatgc acctgtcaat
     7201 gattactata tggaactact gatcatgatc gatgcaatga agcgagcaag tgcgaaaacg
     7261 atcaatgtcg tattgcctta ctatgggtat gctaggcaag accgcacggc gaaacctcat
     7321 gaaccgatca ctgcgaaatt gatcgctaac ttgatcgaag aagcaggggc aactcgagta
     7381 ctgactttag atctgcacac ggttcaagta caaggattct ttgatattcc tgtagataat
     7441 ttatttacga tgccgttgtt tgctcattat taccgtcaat taggtctgac gggagatgac
     7501 atcgtagttg tttcgccaaa aaacagcggt gttcagcgtg cacgtagtct ttcagaatat
     7561 ttgaacagca cattagccat cgtagatcat gcagatgatg cttcaactga gggtggttat
     7621 gtgatcggtg atgttcaagg aaagacatgt atcatggtag atgatatctt gaacacagga
     7681 gcaacgcttg cacgagcagc aaatgtatta aaagaaaacg gcgcaaaaga agtatatgcc
     7741 tgtgcgtcac atggattatt gtccgatcat gcgaaaagta ttttagacaa tgcaccaatc
     7801 aaagatattt gtatcactga ttctgttttc acagaagaaa atcgtcatcc agaaaactta
     7861 acaatcgtta catgttcaga attaatgggt gaagcggtca aacgcatcca tgaaaataca
     7921 ccgatgagtc cgttattccg tctggaagaa aaagaattcg aataaacaaa aaaaagaggg
     7981 aaagaagttg tgacagaagt gagcagcttc aaggagctgc gacaagactt ttgtcacagc
     8041 tccttttttc tatgcccatg aaacgtcctc tagacaaaaa tgagctcttc tttggtttgt
     8101 tcgctatttt atgctagaat gtgcctagca ataatttgga agggatgaat gaagtgaaaa
     8161 ctatttatct ggatcatgcg gcaactaccc ccatgcatcc acaagtaatt gaagcgatga
     8221 cggcaatcat gcaagacacg tttggaaatc cttcaagcat acatggattt ggtcgccacg
     8281 cgcatgaaaa attggaaaca gcaagacaaa tcattgcaga cagtctgcaa gcaaaacctc
     8341 acgagatcat ctttaacagt gggggaacag aaggggataa tacagctatc ttagaaacag
     8401 cgttctcgcg tcaaaaagaa ggcagacatc taatcactac agctatcgaa catcctgctg
     8461 ttcttaatac catgaaatat ttagagacaa aggggtttga cgttacatat ctgcctgtag
     8521 atgaaaaagg gaatctgtcg atcgaagagg tcaaaaaagc gttacgagaa gatacgatct
     8581 tggtctctgt gatgtacggg aacaatgaaa tcggaaattt gatgccaatc aaagaaatcg
     8641 gtgaactttt agcggatcat ccagcttatt tccatacaga tgcggtacaa gcgtacggaa
     8701 accaaacgat ccatccagaa gaattaggaa tcgatttatt aagtatctct gctcataaaa
     8761 tcaatgggcc aaaaggcgtc ggtttcttat ataaacgaga cggagtaaat atccctgcat
     8821 tgcttttagg cggggagcag gaagaaaagc ggcgtgcagg tacagaaaat ttagcaggga
     8881 tctggggtat ggcaaaagca gttgaactgc tgtcacctga agaacgtgaa gcacgtaaca
     8941 acaaatataa aggattccag caattgattc ttgatacatt agaagcaaac gaaatccctt
     9001 atcaagtaaa tggtgattta acgaataaac tccctcatgt gttgaatctt cgcttaattg
     9061 gcgaagcaaa tgatttgcta ctgatgaagc ttgatcttaa tggggtcgct atttctactg
     9121 gttcagcttg tacagcagga aatatcgagc cttcacacgt attagaagca atgtatggag
     9181 aagatacacc gatactaaaa gaatccatcc gtatcagctt tggtttggga aataccgaag
     9241 acgacatcca gtatttcctt gatcaattag ttcgtgctgt caaaaaataa ctagtgaagt
     9301 cattcttata tgaacagatg tctgagagaa cgccttcatt ttttagggaa cttaactgaa
     9361 aaacctagta aaaatcagga taaactttta taataaagag aaactaattt tatcgaggtg
     9421 aaaatcatgg catttgaaac aacagcaacg gtactgggcg caccaattag ctatcgtttg
     9481 catcctgatg caaaaagata tacattgcgc gataatggat ttacagaaac aaacggaggc
     9541 aattatcaat tgatccgacc attagatgcc acgccgcaaa gcaaagaagg attcaaatta
     9601 aaaatcactg tatcaaaaga tattcaaaca ttgaaaatgt cgatcactac agcaaatgga
     9661 ttgaaagcag tcaatatttt caaagatcca aaacagaaaa tgagccaaga taaattttat
     9721 tttctgatgg atgggatgat tagccgtggg ttattcgaaa aagtcgaatg atctgtggat
     9781 ataaaaagac aactagtttt gaaacggcct attgttagat gactaacttt gtaaggacga
     9841 cgcaaattag ttgtcttttt tatttttaaa tttaacacat gttgttattt ttaatatttt
     9901 gctgatttat tgtttctaag taaagaaatt taaattgaaa cgattgttat ttagggaaaa
     9961 aggtattttt tgaaaaaaga atcccgtata atgattgttg tgcaaacgct atacaaaatg
    10021 aaaaagaaaa gagggatgtt tatggaaaat gaacagtcgg tcgttttaac gaattggaag
    10081 agaaattatt tgttcttttt atcgggacag tttttgtcag gaatcactag tatggtcgtc
    10141 caatatgcga tcatctggta tttgacaaga gaaaccggtt cggcgacaat tttgagtttt
    10201 gctactttac taggaatgat tcccatggtt ttactaagcc catttgtagg accgctagtg
    10261 gatcgctggg ataaaaaagc tcttttgatt gtcacggata ttattgtagc gattttcgcg
    10321 cttattttag cagtagtcgg aacgatttca gaatccttcc cgatttggct agtttttgtg
    10381 tctttgttta tgcgttcggt cgcacaaaca ttccaaatgc caacgatcca gtcgattatg
    10441 ccgacaatcg ttccatcttc tcacataaca aggacgaatg gacagttagg aatggtccaa
    10501 tcagcgaatt tcatcattgc gccagctcta ggtgcggctt tgttttcagt cgttcctgtc
    10561 aattacctta ttttattaga tgtattgggg gcagtttttg gtgtcggctt attgattttt
    10621 gtgaagatcc caaaagtttc tcctgaaata ttggaagttc cgcttactat ttttaaagat
    10681 gcaaaatttg ggctgcagca attgatggat aataaagggt tatggtacat aacgatcaat
    10741 ggtgcatttg tgatgctgtt gttcatgcca gcaatcagtc tgtatccatt gatgacgcta
    10801 gattactttg gtggttccgt tggacaggcg ggagcagtgg aagtagtcta cgcagtcgga
    10861 atgctcttag gcggtgcact gatcagtttt ataggaacat ggaaagaccg aatgaaacct
    10921 attattatag cctacatcat tatgggcctg acgatcggtg caagcggatt ggttccaaac
    10981 gatagtcaag ggttcttgta ctttcttatc ttgaatgcag gagcaggttg tgcaacgcca
    11041 tatttcaata cactgctgat ggcaatgatc cagcagagtt atgaatcgaa tgttttaggt
    11101 cgtgtcttag ggaacttcaa ctctttgatg aacctggcag gtccaattgg attattattt
    11161 gcaggaccat tagctgatcg tttaggggta gaaaaaatgt tcttgttttc cggaatcggg
    11221 attttattat gcggaatcgt tttattttta acgtcagctg cccgaaatta tgataaagaa
    11281 ttacagaaaa aactggtaaa agaacaccat gagcaaaaag acgaatagta tggaatggag
    11341 ttgtgacaga agtcttgtta caactccatt tctgatccta atgttcctgt ggcaataagc
    11401 ctactcaaat gaaaaatatg agtaattatc tttatttgtt taatacttga tatagggcag
    11461 ttttttcacg acctatcgta cttcctcttt gcttcataga tttctttgat ttgttgctca
    11521 gataacggag tgccaaatgg tagtttttcc tttaagagtt ctctttccgg tgcgtctacg
    11581 cctacattga catcgttctc gataggtacg ctgtaatgat ggatatgtcc atgaagattg
    11641 cggatagtgt cgttaggccc gagcaacatc ggataatgag tcaagtaata ttgttgatgg
    11701 tcgaatttaa cgatggcacc tacatcataa aaataaaatt tgggacttcc gtcagtaagc
    11761 ctaggatcat ttttttctaa ataacggaaa aaagcgcgac tgtcatggtt ccctttgatc
    11821 aagtggattg ttccttgcag ttgagtgacc agttccaaaa tttctgcatt tgctttttca
    11881 tattgcggat gcatagcaag gtcgcctaga tgataaacat ggtcggtttc ttttattact
    11941 gcattccagc tagtgatcaa ctgttggttc atttcatcca catttgcaaa gagccgcgga
    12001 gcaaagtcgt tttttcctaa taagcgttca tggaaaaagt gcatatcact gataaagtaa
    12061 ttcatgtatc ttttgcctcc cttttttctt agagtaaaca aaatcactta tctgagcaac
    12121 tcataacatc taaaaagaaa aggttgtaaa tgagacgtgc agctttaagc gataagaagc
    12181 aattttgaaa aatagttttt cacaatttat aaaattattc cttattgccg aaaggctggc
    12241 cctttttatg agtatgccaa ttttttatat cgaatgctca aaagatgagt gattttttgg
    12301 ttgactatca tgtttgtgta ctgaataggt ccagtagaac agtattttta cgacttgata
    12361 attaagggat gaaattttca gaaatatgac gtataatgta aaatactgaa aagttgcgac
    12421 cttcaactcg tagatgtttc agaatctaat tgaaatgatg gtgatatcat gacagacaac
    12481 agcaaaacac gcgttgtcgt agggatgagc ggcggtgtcg actcatctgt aacagcactt
    12541 ttgttaaagg aacaaggata cgacgtgatc ggaatattca tgaaaaattg ggatgacacg
    12601 gacgaaaacg gtgtatgcac ggccacggaa gattataagg atgtagcgaa agtagctgct
    12661 caaatcggga ttccttatta ctctgtgaat tttgaaaaag aatattggga tcgtgtattt
    12721 gaatatttcc tagccgaata ccgcgcagga cgtacaccta acccagacgt gatgtgcaac
    12781 aaagaaatca aattcaaagc attcttggat tatgcaatgg atcttggcgc cgattatgta
    12841 gctactggac attatgcgca agtcactcgc gatgaaaacg gcgtggtcca catgcttcgt
    12901 ggagtagaca ataataaaga tcaaacttac ttccttagcc aattatcaca agaacagctt
    12961 tcaaaaacga tgttccctct aggcggcatg gaaaaatcag aagtacgggc aattgctgaa
    13021 cgcgcaggcc ttgcgactgc taagaaaaaa gattcaacgg gaatctgctt tattggtgaa
    13081 aagaacttca aacaattttt gagcaactat ctgccagcaa aaaaaggaaa tatggtcact
    13141 cttgatggtg aagtcaaagg acaacatgct ggcttgatgt attatacgat tggtcaacgt
    13201 caaggactag ggatcggtgg cggaggagat tcgcaagaac cctggtttgt tgtaggaaaa
    13261 gatctagcga caaacacatt atatgtgggg caagggttcc atcatcctgc attgtatgcc
    13321 acaagcttgg atgcaagtga aatccacttt acgacgaatg aaccaatgcc aaaagaattc
    13381 aagtgtacag cgaaattccg ttatcgtcag caagacgttc ctgtgacagt tcgcttattg
    13441 gatgataatc gtgcagaagt cgtattcgac gaacctgtac gtgcaatcac tcctggacaa
    13501 gcagttgtat tttatgatgg aatggaatgt ctgggtggtg gtttgatcga ccacgcatat
    13561 caagaaacaa aagttttaca atacgtttaa gcgatattaa atgaatcaaa acagctatgg
    13621 ctaacaaaag aaacaccttt tcttttggat agcatagctg tttttttgca ataaaattaa
    13681 tttttattca ttttatactt gaaatacttg tgtaaaaagt tcataatatc caataatatg
    13741 aataaagttg ctttttgtta agcaagagca gttgcaaaat agaagggaat cttttgcaag
    13801 tgatttgttc aattttataa gtgagggtga tggaaatgga aaagaaatcg acgggtatta
    13861 gcaaatgggg actggttgct ctggttgtca gttcgtcaat cggaagcggt gtgtttggga
    13921 tcacaagtga tctggctagt tctgcagctc cagggccagc gatcctttca tggataatcg
    13981 ttgggattgg tattttagct ttggtattat cgctgaatca tttaggagaa aagcgtccag
    14041 atctagatgg tggtattttc ggttacgcgg aagcttcatt tggtaaatta ggtggcttca
    14101 tcagtggttg gggttactgg ttgtctgcgt ggctgggaaa tgttgctttt gcaacgatgc
    14161 tgatgagcgc catgggagaa tttttcccga tattcaaagg tggacaaaat gttccttcta
    14221 ttttatttgc aagtattttt atttggggtc tgactttatt agtaaacaat ggagttgaag
    14281 gagcgagcgt catcaataca atcgtgacga tctgtaaatt agtgccatta ttcctatttt
    14341 taatatttgt atttatcgca tttaaagtga atatattcac tgctgatttt tggggtaacg
    14401 tatctgataa tctagtgaat ggaacagggc agcaaggaac gatctggttg cagattaaag
    14461 gttgtttgat ggttatgatg tgggtctttg tagggatcga aggcgcaagt gttttagcca
    14521 accgcgcgaa gaaacgttca gaagcccagc aagcatcgat cattggtctg ctttgtttat
    14581 tgtttatcta tatccttgct tcgatcttgc catacggcgt cttatctcaa gaagagttag
    14641 caactatttc acaacctgct atggcaaata ttttaaaagg aatcgtggga aattggggag
    14701 cagcgctgat caatatcggc ttgattattt cgatcattgg ttcttggttg tcttggacga
    14761 tgctcccagc agaaacaacg atgttgatgg ctcgagacgg tctgttgccg aaaggttggg
    14821 gaaaaacgaa taaaaaaaat gccccaactt attcgttagt tgttactgca atcttgacta
    14881 atctattctt attgactttc ttagttactg attatgccta tcaatttgcc tattctttat
    14941 gtacagcggc aattttgatt tgttatctac ttgtcgggat ctatcaagtc cagttttctt
    15001 taaaagaagg aatcaaaaaa caagtagtga tcggtgggat tgctgtagtt tttgaattga
    15061 tgggcatcat tttagcagga ttttcttatg tccttttatg tagtattgcc tatcttcctg
    15121 gtttttgctt ctactggatc gcttgtcgtg aaaccaaaca tcaaattacg ggaaaagaaa
    15181 aagtaatgat tgggctgatt tgtgtggccg cactgctttc aatcgggttg cttgctgtag
    15241 gctggatcaa gatataaggg ggaaagaaac atgaaaatcg caaatttcgg tgtagaagaa
    15301 tggctaaata aatgggaaaa gaaagcggta tatgatattg cacaaagctc gatcgaagca
    15361 ttgacattag acgaactcgt tggcttagat gggactagcg taagtaaatt tttcgaaaaa
    15421 cgcaaaaacg aaccattgga ctatggctgg atcgaaggat cagatgtttt caaacaagaa
    15481 gtcgcttctc tttatcaaac cgtcgatccg gaaaacatct tacaaacgaa tggagcgact
    15541 ggtgctaatc ttttagctct atacgcgtta gtggaagcag gagaccatgt tgtttcgatg
    15601 cttcctactt atcagcaatt atatgacatc cccaaatctt taggtgccgc tgttgacttt
    15661 gttcatttga aggaagaaga agattggcag tttgatttag aacagctaga agccttagtc
    15721 aaacctgaaa caaaaatcat ctgtttgaat agtgcaaaca atcctacagg cacattgcta
    15781 gatcggaaaa ctttagaaaa aatcgcagaa attgctcgta cagtagatgc atatgtattg
    15841 atagatgaag tatatgcgcc tttaacggat aaaggtgaat tcttgtcgat cgtagatgtc
    15901 tatgaaaaag gtatagcaac caattcccta tccaaaacct actccattcc aggtattcgt
    15961 attggctgga cagctactgg acctgagttg gcagaagttt ttcgaaagta tcgagactac
    16021 acgatgattt gcggcggagt cctgtcagat gatcttgctg tacatgcatt gaagaataaa
    16081 gaaaaaatat tggcgcgaaa caaaaagatc attacagaga accttgtgat tttaaaacaa
    16141 tgggtagcca atgaaccgaa agttgaactt gtcgctccca attacgtctc tacttctttc
    16201 attaaattag ccatcgaaga agatgaccag acattttgta tcaatttatt agaagaaaca
    16261 ggagtactat tagtaccagg aagtgccttt gatttaccga agcatgcccg actaggttac
    16321 tgttgtaaaa aagaaacctt ggaaaaggga ttattcctct tatctgagtt cttgaaaaaa
    16381 caggcctaaa caaaaacact gcaaggacag tctgtgacaa ctgttcaagc agtgtttttc
    16441 atttacgctt attgtctatg agaaggagag tgccctgaag ctatggagac tcctgaagct
    16501 ttttcttctg cagtttcttg ctgatggatc tcttgtgtat tgtgtcgtac aagcaagaag
    16561 ttgaccaaaa caagaagaga agtcgaaaga aagacgccgc gataaccaaa tgcactggaa
    16621 acactggagc cgatcatagg ccctactacg ttaccagttg cttggaaaga ttggttataa
    16681 ctgaagatcc gtccagcagc ttgatgaggc gaatattttg tgatcaatgc ttggacagca
    16741 gggagcagac aagcatcaga gataccaatc aaaaaacgta aggcagctaa ttgccaaaca
    16801 ttcgtgacga atgccattgg gatataaacc agaatcgcaa aacccaaacc aatcgcaagt
    16861 attctttcac tcccaatctt gtcacctaag cgaccaaatc ttggagcggc tatcaaagtg
    16921 gcaatcccag gtatcgaagc aatgattccg ctgatcaacg taacattccc atgtccatgc
    16981 agcaattggc gaatgtacag actgatgatt ggactaatgg aattgttgga tgcttggatg
    17041 atcatcgttg tgacgaacat accgatgacg acgtgtggat atttcaagtc tttgaaaatc
    17101 tgtttggcag aaaccatgtt ttcttttttc actgggacaa atttttcatg gacgaaaaac
    17161 agactcaaca agaagacgaa aaataagatg atccctgtaa tgaaaaaggt agggcgatag
    17221 ccaaacgctg aagcagagat cccgccaaga agcggtccaa gtaatgttcc tgtaacagaa
    17281 ccagttgcta aggttccaag aacttgacca cttttttctt ttggcgtccc agttgcgaca
    17341 agggcagtgg cattactgat ataaccagaa aaaatccctt gaagcagacg caatgcgact
    17401 aattgataca cactagtcac gcaacccatc aaagaaatga cgattgccat tcctaatgaa
    17461 gcacgcaaca gcatcaattt tcttcctttt tggtcggcta attttcccca ccaaggagag
    17521 ataagcgtag tcaccaagaa agtagatgaa aaagtcaatc cgctccaaaa gttcaattgt
    17581 gacgtactat aattacctaa cgtatctatg taaagtgaca taaaaggcat gactaaacta
    17641 aatccgatac cagccataaa tgttccaaac cataagacaa acaagttttg ttcccaagtt
    17701 tctcttttcc taaataattt ttcctttatt cttgcagcca attagactcc ttctttctga
    17761 aaattttcat ctacagctct ttattataaa ctacaatatg tagtttatca atgaaagtct
    17821 cttttttctc tttctttttc gtggtaaact gaaagagtga aggatttatt ttatgagagg
    17881 aaacatggaa atgaaaaaaa gaaatctgtc tcaagagaaa atcatcgact gctttcgaga
    17941 actagcagaa gagatggatg ttcagcaaat aacctttcag catttagcca aacgcttgga
    18001 tatcaaggct ccctcgctgt ataaccattt caaaaatatt cgtgatgtca aaacagcgct
    18061 aacagcgaaa cttctgcagg aacttaatga ccagctgcga cgggctcttg tgggaaaaag
    18121 tggtcaggaa gcactgcaga tctatgcaga aacttatcga tcctttgctt tttcaaatca
    18181 agcagtgtat gagttactga tcagtgtacc gcataccaat gaagctgttt tattagatgg
    18241 aatccatgaa acgaatcaga tcattttgca gatatttgag gcttttcaat taacgaaaga
    18301 agaaaaagtt catcgaagca gggaacttcg tagtatgatc cacggttatc tttccttacg
    18361 ttttttagga tactttacaa aagaaccaac aatcgatcca gaagacagct atttttggat
    18421 gatcaataac tttatcgcta ctttgcctta aacaatcaga ttcatttgta acaattttaa
    18481 gatgttcatt aaatgaccct tttataagag aaaattcttg caactttctt gaaaagtgac
    18541 taagatgagt ctagaaaagc tgaaagaagg agcacgagta tgtatcaagt aattaccatg
    18601 ttcggggaca atgagccttg gtggtttttt gaagactggg aagaagatat tgaagaagaa
    18661 gaaacattcg agtctttcga agaagcacga caagcgtacg aaaaacaatg gaacgaaatc
    18721 catcaaaatt atgaatatat caatgcaaaa agtaactttc tcagcgcctt ttggaatgac
    18781 ggagatgagc gctggtgtga agagtgtgac gatgatttac agcaatacaa gggacttgcg
    18841 ttgttgaaaa atcatcagcc catcacagta gaaagcagaa aggaatttta tgaaacaact
    18901 aattctagcg gaaaaaccaa gcgttgcgaa agacctaagc aaggtgcttg gtgctaatca
    18961 aaagcacaaa aattattatg aaggacccaa ggtgatcgtt acttgggccc ttggtcattt
    19021 attaggattg aaaatgccgg aagatctgaa taaggaatgg cagagctggc aaatggacac
    19081 gttgccaatg atccctaaaa aactaggaat caaaccactt cctaaaacag gacatcaatt
    19141 aaaagcaatc aagcagctgg ctcagcgaaa agatgtctca gaagcagtca ttgcgacaga
    19201 tgccggaaga gaaggggaac ttgttgctcg ctggatctta gagtatgtgc atttcaataa
    19261 accagtgaag cggttatgga tctcttctca aacagataaa gcaatcaaaa ctggattcaa
    19321 gcaactaaga cctgcgaaag aatatgatgc attgtatgaa tctgctcttg cccgggcaaa
    19381 agcggactgg ctggtaggat tgaatgtgac cagagcctta actgtgaagt acgcggataa
    19441 tttatcagca ggccgtgtgc agacaccgac actagcatta gtgagagacc aagaaaagaa
    19501 aatcgagcag ttccgtccac aaacgtattt cacgatcacg ttgcaagctg gaaaagaaca
    19561 agccaaaatg cttcaaaaaa atcaatttgc tttaaaaaca caagaagaag cacaaaaact
    19621 tgttcaattg atcagtacaa gcaaaggaac tgtcacagaa gtccaagaaa aattaaaaac
    19681 ggaatctgcg ccattgcctt atgatttaac agagatccaa agagaagcca atcaacgcta
    19741 tggctattct gcaaagaaaa cattaagtct tgtacaaagt ttgtatgaga cccataagat
    19801 cgttacttat ccgcgtacag acagcaaata tctgacaaat gacatgaaaa gtacgatgaa
    19861 agaacgattg caagcagtca gcgactttgc tcctgaggtc aagcagtatt tgaaaaacgg
    19921 agccatcgtc caacagacaa aagtattcca agacagtaaa gtgaccgatc accatgcact
    19981 tttgccaact gaaaacagag cacgttatga aaaattaagt aatgaagagc gaaaaatcta
    20041 tcaaatgatt gtctctcgtt ttttaggact gtttgctcaa ccccataaag tcagccaaac
    20101 aaaagttacg gttgaatttg gtaaagaacg attcattttt cggcaaaatc gtgtcgtcca
    20161 agcaggatgg aaaggcgaaa ctgaaactga gacggaaaca gtcaaatggg aaaaaggaat
    20221 gagaatcaat cccgatttcg ccatcaaaaa agaactatct gcaccgccta aaccattgtc
    20281 agaagcaagc ttgcttggtc aaatggaaaa atacagtctg ggaaccccag ctacgcgtgc
    20341 agagattatc gagaaactaa tcaaatctga actgatggaa cgtacccctc aaggactgca
    20401 agtatctcct aaaggtaagc aattattagt attggtaaat ccctctttag taacaccaga
    20461 tttgacagaa agctgggaaa aagaccttga agctattgct gcaggaaaga aacaagcagg
    20521 aaccttcttg aatggaatcg aaaaagaaac aaaacgcttg gtgaatgaga tcaaatcaag
    20581 caaacaagaa tatcaagatt tctcgattac ccaaaagaaa tgcccagaat gtggggccaa
    20641 tctgcgcgag aaaaacacac gtgacggcaa aatctatgtc tgcaccaatt cagattgcaa
    20701 ctaccgcaga agaaaagatc caaaagtctc taatcatcga tgcccgcaat gtcaccggaa
    20761 aatggaaatc atcgaaggta aaaacggtgc ttatttccgt tgtaaatacg atggaacgac
    20821 agaaaaattg cttgataaaa aagagcagaa gaagaaaatg accaaacatg aagaacgacg
    20881 cttgctgaaa aaatatgcac aagcagaaga accagaagaa agcccattag cagcagcact
    20941 aaaagcagca atgaagaagt aataagatat gaaagaggtt gtgatagaag tggtcagctt
    21001 caagaaataa gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa tttcggctta
    21061 tttccgaaga agctacttct ggaacaccat ttattcggaa aaaaggactg tgacaagact
    21121 tttgtcacag tcctttttca tttaaaaaaa tgtttacttc atttgacgta tattattggt
    21181 gaggcttcaa ttcatttggc ttcacttttt ctgtttctct tatgatataa acaggacgct
    21241 gtttcgtttc taagaagatt ttcccaatat atttcccgat gattccaaga cataacaatt
    21301 ggattccacc aatgaacagg aagatcgaga ccatggaagg ccatcctgat gtaggatcac
    21361 caaacaacaa tgcccgcaaa ataatgaaca aaattgcaat accagaaccg acacaagaca
    21421 aagcgccgat aaatgaagca atcgttaacg gtgcatccga gaagttcacg ataccatcaa
    21481 tcgagtagtt gaataatttc cagaaggacc aagatgtttc gccagccaca cgttcgcggt
    21541 tttcataaga aatatattct gttttaaatc cgacccagct aaataatcct tttgaaaatc
    21601 ggttgtattc tgttaattct aggacggcat ccaccatttg acgagtcatc aagcggaaat
    21661 cacgagcgcc atcgaccatt tctgtatcgc tgattttatt gatcaactgg tagaattttt
    21721 tcgcaaagaa actgcgtatc gcaggctcgc cttcacgagt cgaacgccgg gtgccgacac
    21781 aatcgatatc agaagtagtg atccggttga tcatctcagg aagcagttcg ggcggatctt
    21841 gcagatcaac atccatcacg gtcactaaat ctcctgtaga agcttgcaat ccagcgtaca
    21901 gagccgcttc tttcccaaaa tttctggaaa atgagacaaa gcggacataa ggataagctg
    21961 aggctagctt cttgattgct gctaacgtac cgtcttttga tccgtcgtca ataaacaaat
    22021 attcgaactc atgggcacct ttgaattttt caacctcatc aaaaaaaaga ggaacagttt
    22081 tttcttcatt aaaacatggg acaacaattg atactttcat cgtttctatc ctttcaatcg
    22141 tatgtttata tatggtttac attcaaaatt taataaacct taaataacac ctttaaaatt
    22201 tgattttacg taaaagaaca ggtattcttt ttgtaaacta acgtatttag tttaccatgt
    22261 ttcctcggtt tatcaaactt ttaagaacaa tttttattag ttggcaagta gttttggata
    22321 gagaaaaagg aggaagttcc agtctattga ctaggagcaa acaaaatgaa aatacacaca
    22381 cctatcggct ttttatggtt agatgcagat gaatccggat tgactgctgt atcttttcag
    22441 ccaatttcag aaagcgaatc agcgaatcag cgaattttaa cgcgcacaca agaacagttt
    22501 gaagaatatt ttgcgggaaa aagaaaagaa tttgatgttc ctttatctat aaagagaggt
    22561 acgccttttc agcaaaatgt ctggaaagct ttgtcgacca ttccttatgg agaaatccga
    22621 acgtataaag aagtagccgt tgctgttgat agtccgaaag ctgtgcgagc gattgggcag
    22681 gcaaatcgaa ccaatccatt gccaatcgtt attccttgtc atcgagtgat cgggcaaaat
    22741 ggaaaactga caggatatat gggaaacgca atagatgggg ttgaaatcaa acgccaatt
//
LOCUS       DAZAID010000045        22739 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000045 DAZAID010000000
VERSION     DAZAID010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.045"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..1027
                     /gene="guaC"
                     /locus_tag="ACG9BE_001571"
     CDS             50..1027
                     /gene="guaC"
                     /locus_tag="ACG9BE_001571"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFN3032316.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            1291..2715
                     /locus_tag="ACG9BE_001572"
     CDS             1291..2715
                     /locus_tag="ACG9BE_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFN3032317.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(2757..3014)
                     /locus_tag="ACG9BE_001573"
     CDS             complement(2757..3014)
                     /locus_tag="ACG9BE_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032318.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            3176..4285
                     /gene="pdhA"
                     /locus_tag="ACG9BE_001574"
     CDS             3176..4285
                     /gene="pdhA"
                     /locus_tag="ACG9BE_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFN3032319.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            4288..5265
                     /locus_tag="ACG9BE_001575"
     CDS             4288..5265
                     /locus_tag="ACG9BE_001575"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFN3032320.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            5290..6933
                     /locus_tag="ACG9BE_001576"
     CDS             5290..6933
                     /locus_tag="ACG9BE_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFN3032321.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            6941..8347
                     /gene="lpdA"
                     /locus_tag="ACG9BE_001577"
     CDS             6941..8347
                     /gene="lpdA"
                     /locus_tag="ACG9BE_001577"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFN3032322.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            8509..8784
                     /locus_tag="ACG9BE_001578"
     CDS             8509..8784
                     /locus_tag="ACG9BE_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFN3032323.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            8790..9557
                     /locus_tag="ACG9BE_001579"
     CDS             8790..9557
                     /locus_tag="ACG9BE_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFN3032324.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            9725..11554
                     /gene="typA"
                     /locus_tag="ACG9BE_001580"
     CDS             9725..11554
                     /gene="typA"
                     /locus_tag="ACG9BE_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFN3032325.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            11815..13587
                     /gene="recQ"
                     /locus_tag="ACG9BE_001581"
     CDS             11815..13587
                     /gene="recQ"
                     /locus_tag="ACG9BE_001581"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFN3032326.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            13701..14003
                     /locus_tag="ACG9BE_001582"
     CDS             13701..14003
                     /locus_tag="ACG9BE_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFN3032327.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            14006..15169
                     /locus_tag="ACG9BE_001583"
     CDS             14006..15169
                     /locus_tag="ACG9BE_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFN3032328.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            15195..18623
                     /locus_tag="ACG9BE_001584"
     CDS             15195..18623
                     /locus_tag="ACG9BE_001584"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFN3032329.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            18644..19768
                     /locus_tag="ACG9BE_001585"
     CDS             18644..19768
                     /locus_tag="ACG9BE_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFN3032330.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            19749..20195
                     /locus_tag="ACG9BE_001586"
     CDS             19749..20195
                     /locus_tag="ACG9BE_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN3032331.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            20179..20517
                     /locus_tag="ACG9BE_001587"
     CDS             20179..20517
                     /locus_tag="ACG9BE_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFN3032332.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            20636..21196
                     /gene="rsmD"
                     /locus_tag="ACG9BE_001588"
     CDS             20636..21196
                     /gene="rsmD"
                     /locus_tag="ACG9BE_001588"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFN3032333.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            21193..21684
                     /gene="coaD"
                     /locus_tag="ACG9BE_001589"
     CDS             21193..21684
                     /gene="coaD"
                     /locus_tag="ACG9BE_001589"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFN3032334.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            21674..22717
                     /locus_tag="ACG9BE_001590"
     CDS             21674..22717
                     /locus_tag="ACG9BE_001590"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFN3032335.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         7715 a         3927 c         4999 g         6098 t
ORIGIN      
        1 ttaataatgt tcgtgttttg ctggatgtga acatcaggag gataatgtaa tgaaagtatt
       61 tgattacgaa gatattcaat taattcctaa taaatgtatc gtaaatagcc gttctgaatg
      121 tgatacaacg gtcacattag gtaaacatac attcaaaatg ccagtagtgc ctgccaacat
      181 gcagacgatt attgatgaaa caatcgcaga attcttagcg gaaaatggtt atttctatat
      241 catgcatcgg tttgatgaag cagctcgaat tccgtttatc aaaaaaatga aaaagcgtgg
      301 attgattacc tcgatcagtg ttggcgtcaa gaaagaggaa tattccttta tagagaagtt
      361 ggcagaagaa tcgctaaatc ctgattatat cactatcgac atcgcgcatg gacatgctaa
      421 ctcagttatt gatatgattc agcatattaa aaaatatttg cctgaaactt ttgtgattgc
      481 cggtaatgtt ggtacaccgg aagccgtccg tgaattggaa aatgcgggcg cagatgcaac
      541 aaaagtcggg attggtccag gaaaagtatg tattacaaaa atcaagacag gctttgggac
      601 tggaggctgg caactggcag ctcttagatg gtgtgcgaaa gcagctcgca agccgatcat
      661 cgctgacggt gggatcagaa cacatggcga tattgcaaaa tctgtccgtt ttggtgcgac
      721 aatggtcatg attggttcat tgtttgccgg tcacgaggaa tctcctggcg aaacaaaagt
      781 cgaaaatgga atcgtgtata aagaatactt tggttctgct tcagaattcc aaaaaggcga
      841 aaaacgaaat gttgaaggaa agaaaatctg gatccaacac aaaggatctt tgaaagatac
      901 actagtggaa atgcaacaag accttcaatc atctatttct tatgctggag gaagagatct
      961 ggaagccatt cgaaaagttg actatgtcat cgtgaaaaat tcgattttta atggagacac
     1021 aatctaaaaa ataaataaaa tattaggatg ttgtgaaaaa gttgtcctgc attaagtaat
     1081 aagaacagcc aaacaaaaat agttctttat atgttttgtt tggctggaat tattatcgca
     1141 gatacaagct tttgaacatc attattcaga ataagggact gtgataagat ttttgtcaca
     1201 gtcccttatg ttttgcaaaa aaagtttttc ttttttctcg taaattcggt aaaatcaaaa
     1261 gaaaaaatag acaatagaaa agagaagctt atgtgggaaa aagtagagaa aaaacaagga
     1321 ccgatttatc gtcaaattat ggaacagatt atgagagaaa tcgaaaacgg aaggatcaat
     1381 cctggaagtc aacttgaatc agaacgaaaa ctagggcaac tttttggtgt gaatcgttcc
     1441 accgttgtcc atgcattgga agagcttagg gagcttgggg tactaacgag caaacgtggc
     1501 agcggatggt ttgttaatca gactgaatgg gggaaattcg ctgttccaag aatcgattgg
     1561 cggcaaatga tctctcctcg ttatgagcag acggactggt atgagcaaaa aataagagaa
     1621 gcaaagcaga tccaaaaaac atcgtttctt gatttatacg ctagcgagat gccagatgag
     1681 cttttgccga attttgagtt tccttcttat tctcttgaag aaatcttagc ggaagaaaaa
     1741 gaaatggatc ttttaggcta tactccgtta cgtaacaaaa tcaaacggta tttggaaagt
     1801 aaatttgaat tggcattttc ccccaaccag cttcttgtga ctggtggcgg tcagcaagca
     1861 atctttttga ttttacaaac gattctttca gctggagagg ggatagctgt agagtcacct
     1921 tctttttttt accgattacc tcttttcaaa gcttcaggaa cgcgtttatt tgggataccg
     1981 atggatgaag aagggatcga cttagaaaaa ctgaaagaat cgatccataa aaataaattg
     2041 aaagcagtgc ttatcaaccc gaattttcaa aaccctacag gaaaagtgat gagtcaaaaa
     2101 cgacgagaaa agctagtaga attgtgtcga cagtatcggc ttccaattat tgaagacgat
     2161 gtatttacgg atctttcatt tctaaaccaa gaagaagtcc ttcctatacg aacgattgat
     2221 ccggataatg tgttatacgt cggttcgtta tctagggttt taggaaaaac aacgaaaatc
     2281 ggttgggtga taggacctga gacttttatc tcgcaactag ctcaagcgca agaaatgatg
     2341 gagttttcga tgaatatcct tacacaaatc acagtggcta atgtatttaa tgaagcaatt
     2401 gatttgaaaa tgcaagatgt aagaaaaaag ttgaaagaaa aaagccgact gataagcgct
     2461 tggggagatt cacaatcgtt tttcaagctt cattcaattt taggtggtta ctatgcctgg
     2521 attacttggg atgggaaaaa actagacccc gaactagcag aaaaagtagt agatatgggt
     2581 cttggaattg caccaagtct actttttggg caagatataa atggaatcag aatcaattac
     2641 agtaggctgg atgaaaacca aataccgttg ttttattcaa agatgaaaat tctagaaaag
     2701 tggttaacag aataatttca caaagtaaat agattaaaga atataacagc ttggatttaa
     2761 atttcaagct gtttcttttt tttcggaaaa agaatacgat ataaagaaac taagaaaaaa
     2821 ccattatttg tacgtccgtt cacgatgtgt aaataaaata aatgtttgca acaaaactgt
     2881 ataacaccaa cgtttttaaa tagaaacaga tggttgtaag tttgattaga aaatagaaaa
     2941 aatgcagatg aaatataaaa cgctttcctt attgtgattt tattcactaa tgctgttaat
     3001 atgcggattg gcataatttc ctttcatcgt gaaaatttgt gcttttagct tgtatttctc
     3061 ataaatccat gataatttat taatgaagac ggttaaatag tgatacagtt ttattcactg
     3121 tttcaatcgc agaatgaaga gattttctta tagatgagag aggaatgtgt gacaaatggc
     3181 aaacaaaaaa gcaccgatcg acttccaagc actgctagat gaagtcaatg cagatttccc
     3241 agtatatcaa gcacttgatc aagatggaaa cgtagttaac aaagatttag tacctgattt
     3301 atccgatgat gaactcgttg aattaatgac tcatatggta tggtctcgtg tattagacca
     3361 acgttcaaca gcgctgaacc gccaaggacg cctaggcttt tttgcaccaa ctgctggaca
     3421 agaggcaagt cagctggcta gtgcttacgc atttgataaa gaagatgttc ttcttccagg
     3481 ttatcgggat gtacctcaac tagtaaaaca tggtttacct ttatctcaag cttttttatg
     3541 gtctcgagga catgctgcag gtaattctta tccggaagaa ttgaaagctc taccaccaca
     3601 aattatcatc ggtgcacaat atgtacaagc agccggtgtt gcattaggtt tgaagaaacg
     3661 aaacaagaaa aatgtcgtct tcacttatac tggtgatggc ggttcatcac aaggtgattt
     3721 ttacgaagca atcaatttcg ctggcgctta ccatgcaaat gccgtatttt atatccaaaa
     3781 caacgggtat gcgatctcta cacctcgtga aaaacaaact gcagcaaaaa cacttgcaca
     3841 aaaagctgtc gcagctggga ttcctggtat tcaagtcgat ggaatggatc cgcttgcagt
     3901 atatactgta tctaaaatgg cacgggaatg ggcgatcagt ggaaatggac cagtcctaat
     3961 cgaaacactg acttatcgtt atggaccaca tacactatct ggtgacgatc caacccgcta
     4021 ccgtacaaaa gacacagatg acgattggca gaaaaaagat ccattgatcc gcttccgtaa
     4081 atatttgacg gaaaaaggac tttggtctga agaaaaagaa gaacaagtca tcgaagcaac
     4141 taaagaagaa atcaaagcag ctattgctga agccgacaaa gtaccaaaac aaaaggtttc
     4201 agatttcttg aaaaacatgt tcgaagtatc tccacaatca atcaaagaac aaattgcaat
     4261 ttatgaagcg aaggagtcga aataatcatg gcacaaaaaa caatgatcca agcaatcaca
     4321 gatgccttag cacttgaact agaaaatgac gagaatgtcg ttgtttttgg tgaagatgtt
     4381 gggaaaaacg gaggagtttt ccgggcaact gaaggattgc aggaaaagtt cggagaagac
     4441 cgagtattcg atactccttt agctgaatct ggaatcgcag gattgagttt cggtcttgcg
     4501 ttagaaggat ttcgtccagt tcctgaaatc caattctttg gttttatttt cgaagcaatg
     4561 gacgaagtag tagctcaaat ggctcgtaca agatatcgaa tgagcggcac tagaaatcta
     4621 cccatcacaa ttcgttcgcc atttggtggc ggtgttcata caccagaatt gcattcggat
     4681 aatttagaag gtttgatcgc tcagtctcct ggtattcgag tagtgattcc ttccaaccct
     4741 tatgatgcaa aaggactatt gatttcttct attagaagta acgatccggt tgttttccta
     4801 gaacacatga aactatatcg ctcattccga gaagaagtgc cggatgaagc ttacgaagtt
     4861 cctttggata aagcagcagt tactcgtgag ggaacagatg tatctattat cacatacgga
     4921 gcaatggtgc gtgaagcaat caaagcagca gacaatcttg caaaagaaaa tatatctgtt
     4981 gaaatcattg atcttcgtac agttgcgcca ttagatgtag aaacgatcat tcaatccgtg
     5041 gaaaaaactg gtcgcgtagt tgttgtccaa gaagcacaac gacaagcggg cgttgccgcg
     5101 caagtagttt ctgagatctc agaacgcgct atcctttcat tagaagcccc aatcggacga
     5161 gtttctgcac ctgatacagt cttcccattt ggacaagcag aaaatgtctg gttgccaaat
     5221 gcaaaagata tcgaagacaa agtaaaagaa atcgcggaat tttaagaaaa gaagggacga
     5281 aacaaagtca tggcttatca atttaaattg ccggatatcg gtgaaggaat cgcagaaggt
     5341 gaaatcgtca aatggttcgt caaaccagga gatacgatca atgaagatga tacattacta
     5401 gaagttcaaa atgacaaatc agtagaagaa atcccgtcac ctgttacagg aacggttaaa
     5461 aatgtgattg ttccagaagg taccgtagca aatgttgggg atgtattagt agaaatcgat
     5521 gcaccaggac acgaagacaa tgaaggggac agtggtgtag ccgcagaatc ccaaactcca
     5581 gctaaaccgg ctgctgaacc cactgttgat acagaatcag ctggatcgtc aagcgaagga
     5641 gtcttccaat ttaagttacc ggatatcggt gaaggaatcg cagaaggtga aatcgtcaaa
     5701 tggttcgtca aaccaggaga tacgatcaat gaagatgata cattactaga agttcaaaat
     5761 gacaaatcag tagaagaaat tccgtcacct gtcacaggga cagttaaaaa cgtgattgtt
     5821 ccagaaggca ctgtagcaaa tgttggggat gtattagtag aaatcgatgc tcctggacac
     5881 aatagtgcac cgtcaactag tgcgccatca gctgaagcac caaaagaaaa agtggaaaca
     5941 tcaggttcag caagtgtcgt tgaagctgcc gatccaaata aacgtgtatt ggctatgcca
     6001 tctgttcgtc aatttgcccg tgaaaaagat gtagatatct cacaagtaac tgcaactgga
     6061 aaaggtggca gagtaacaaa agaggacatc gaaaacttct tggcaggtgg tccaagcagt
     6121 gcaccagcga aatcagaagc gccagaagcg gcagcaccga aagaagcagc tcctgcagca
     6181 gaaagcaaac cagctgcacc agcaaaacca ttcaagtcca acttaggaga cttggaagaa
     6241 cgtgtagcaa tgacgccaac acgtaaagct atcgctaaag caatggtcaa cagtaagcat
     6301 acagctccac acgtgacatt gcatgatgaa gtagaagtgt ctaaactatg ggataatcgg
     6361 aaacgattta aagaagttgc tgctgcaaat ggtacgaaac ttactttctt gccatatgtc
     6421 gtgaaagctt taacagcgac tgttaagaaa tacccagtat tgaatgcatc tatcgacgat
     6481 gcaaaccaag aaatcgttta taaacattac tacaatatcg gtattgcaac tgatacggat
     6541 cacggactat atgtaccaaa tgtgaaagat gcagaccgca aaggaatgtt tgctattgca
     6601 gacgaaatca atgagaaagc aaaattagct catgacggca aactatctgc agaagatatg
     6661 cgcaatggaa caattacgat cagcaatatc ggatctgtag gtggcggttg gtttacacca
     6721 gtcatcaact atccagaagt agctatttta ggtgtaggaa cgattgcaca acaaccaatc
     6781 gtaaatgctg aaggcgaaat cgttgtcggt cgtgtgatga aactgtcatt aagtttcgat
     6841 caccgtatcg tagatggagc aactgcgcaa caagcaatga ataatatcaa acgtctttta
     6901 gctgatccag agctattaat gatggaagga tgataaacaa atggtagttg gagattttgc
     6961 tgtagaatta gatacagttg taattggtgc gggacctggt ggctatgtag ctgccatccg
     7021 cgctgctgaa atgggacaaa aagtagcaat cattgaaaga gagtatattg gcggtgtttg
     7081 tttgaatgtc ggctgtattc cttcaaaagc actgatagct gcaggacacc attatcaaga
     7141 gtcactagat tctgaaatgt ttggagtaac tagtgaaaac gtcaaactag attttgcaaa
     7201 aacacaagaa tggaaagaaa acaaagttgt ccatacgttg acttctggtg ttggtttcct
     7261 attaaaaaaa cataaggttg aaacaatcga aggagaagcg ttcttcgtag atgatcacac
     7321 actacgcgtc attcatccag attcagctca aacttattcg ttcaataacg caatcatcgc
     7381 aacaggttcc cgtccaatcg agattccagg atttaaattc ggaggacgtg tgttagattc
     7441 tactggcgga ttagcattga aagaagtacc taagaaattt gtgatcatcg gtggcggtgt
     7501 gattggtgcc gaattgggcg gagcgtacgc aaacttagga gcagaagtaa cgattcttga
     7561 aggctcacca caaattttac caacttacga aaaagatcta gtaaaattag tagaagatga
     7621 tttcaagaaa aaaggcgtaa cagtcgtaac gaatgcaatg gcgaaagaag cagtcgataa
     7681 cggtgacagc gtgacagtga aatatgcagt ggacgggaaa gaagaatccg ttactgctga
     7741 ttatgtgatg gtcactgtag gtcgtcgtcc gaatacagat gatatgggct tagaacaagc
     7801 gggtgtcgaa gtaggagaac gaggattgat cacggtggat aaacaaggac gtacgaacgt
     7861 acctaacatc tatgccatcg gtgatatcgt tccaggggcg gcattagcac acaaagccag
     7921 ttatgaagca aaaatcgctg ctgaagcaat ctctggcaaa aaagtagctg tagattataa
     7981 agcaatgccg gcagtagcct tcactgatcc tgaattggct tctgtaggta tgacgatcaa
     8041 agaagcaaaa gatgctggta tcgaagcaaa agcatataaa ttcccatttt cagggaacgg
     8101 tcgggcatta tcgcttggta aaacagaagg attcatccgt ctagttacga cgatcgaaga
     8161 taacgtgctt attggcgcgc aaatcggtgg agtcggcgca agtgatatgg tctctgaatt
     8221 agctttagca atcgaatcag gaatgaatgc tgaagatatc gccttgacga ttcatcctca
     8281 tccatctctt ggcgaaatcg tcatggatgc ttcggaatta gcattaggtt taccaatcca
     8341 tatttgattt gaataataca ggcgaaacgc aactgaaaaa tcagttgcgt tttgtttttt
     8401 agaattatca ataatattat tatgtaaact taaaaatatg acaacttctt ggtttattac
     8461 cacttcctat gctacactga agaggaatat gaaataggag agttgaagat gcaagaatat
     8521 cagtatccac ttgatttaga ttggacaaca gacgaaatgg tcattgtaat gaagatgtgg
     8581 gaagatctgg aacgtgcgaa tgaaaaaggg ataaacaatg aagctttttt gaagacttat
     8641 cagcagttta aaactgttgt caaatccatt ggtgaggaaa aacgattggg acgggaattt
     8701 gaaaaagctt ccggttattc tttatatcgt acagtgcagc aagcaaaaaa aaatccaaac
     8761 aaaactttga gaatgaacgg gtgagataaa tggaacagat gattgatcat atcaagtcct
     8821 ggcttgaaga agctggtgag aaaatcaaat cttctcaaca aacattaaca atctcgcaaa
     8881 aatcaaatcg aacggatcta gtaacaaatg tcgatcgaaa catccagaat tttctaatcg
     8941 aaaaaatcca ttcctttgat ccagaggcga aaatattggc agaagaagaa gggtgtaatc
     9001 aattatcgga tctcgatgga agagtcttta tcatcgatcc tattgatggc accttgaatt
     9061 ttgtgattga aggggaaaat ttttgtatta tgctcgctgt ttatgaatcc ggcgttggaa
     9121 agcttggttt catttatgac gtgatgagaa atgaactttt ttggggtgga aaaggaatag
     9181 gcgtgtataa aaatgatgag ccattgccta gaccagcaaa taaatcctta tcggaaggct
     9241 tacttggtgt gaatagttat cttttcggtc ataataaata tggaatccga gaaattgcgg
     9301 aaagcagcat ggggacacgt atgtacggat gcgctggtct tgaactagtg gcaatcttga
     9361 aaggaaacca cattgcttat atttcaaacc ttagcccttg ggattatgca gcgggaaatg
     9421 tcttattgga agagtttggt atggtaacaa gcggtctttt tggtactcct ttgacatttg
     9481 aaaaaaggga atactattta gcagggactt cttgtgcata tgcagaaatc gcagaccttt
     9541 tgaaaaaaga aggctgaaaa aaaggtagaa atcattcaca aaaatgataa aaacgagcaa
     9601 aagattaatt ttctcgaaaa aatgaaaaca ttttactata tttcatgaaa aaagtctgct
     9661 ataattagca gtcgagtaaa atttgccctg tcataaagaa ccaataaaaa aggagttagt
     9721 tacattgaat tatagaaacg atatccgtaa cgtcgcaatt attgcccacg ttgaccacgg
     9781 aaaaacaaca ttagttgatg aactattaaa acaatcagat acattagacg cacatacgca
     9841 attgcaagaa cgtgcgatgg actcaaatgc gctagaaaaa gaacgtggta tcacgatttt
     9901 agccaaaaac acagctgtag actataaagg tattcgcgta aacatcatgg atactccagg
     9961 acacgcggac ttcggtggcg aagtagaacg gatcatgaaa atggttgacg gcgttgtgtt
    10021 ggtcgtagat gcgtatgaag gaacaatgcc tcaaacacgt tttgtattga aaaaagcact
    10081 ggaacaacat atcacaccaa tcgttgtcgt aaacaagatt gacaaacctt ctgctcgtcc
    10141 tgagcatgtc gtagatgaag ttcttgaact attcatcgaa ttaggagcag atgatgatca
    10201 gttagacttt cctgtgattt atgcatctgc actaaatggt acgtcaagct tgtcagatga
    10261 tccagcagat caagaaccaa ccatggcacc tatttttgat acaattattg aaaaaatccc
    10321 tgctcctgtc gataacagcg acgaaccatt acaattccaa gtttcattac ttgactacaa
    10381 cgattatgta ggtcgtatcg gaatcggacg tgtatttaga ggaacaatca aagtaggaga
    10441 ccaagtagca ttaattaaat tagacggaac agttaagaaa ttccgtgtaa cgaaattatt
    10501 tggtttcttt ggactaaaac gtctagagat tcaagaagca aaagctggag acttgattgc
    10561 tgtctcaggt atggaagata tctttgtcgg tgaaacagtt actcctgtgg atcatcaaga
    10621 tgcgttacct atccttcaca ttgacgaacc aacattgcaa atgactttct tagttaataa
    10681 ttcaccattt gccggtcgag aaggtaaatt cgtaaccgct cgtaagattg aagaacgttt
    10741 gatggcagaa cttcaaacag atgtatcctt acgagttgaa ccaacaaact ctccagatgc
    10801 atggacagtt tctggtcgtg gagaattgca tttgtctatc ttgattgaaa acatgcgccg
    10861 tgagggatac gaattgcaag tatctcgtcc agaagttatt gaaaaagaaa tcgacggtgt
    10921 aaaatgcgaa ccgtttgaac gcgtgcaaat cgatacacca gaagaatata tgggtagtgt
    10981 tatcgagtcc ttgagcttac gtaaaggtga aatgcaagat atggtccata caggaaacgg
    11041 acaaatccgt ttgacattct taacacctgc ccgcggtttg attggttatt caactgaatt
    11101 cctttcaatg acacgtggtt acggaatcat gaaccatact tttgaccaat atctgccaat
    11161 gcttcctggt caaattggcg gacgtcacca aggggcgctt gtttcgatcg atacaggaaa
    11221 agcaacgact tattctatca tgagtatcga agaacgcggt acagtcttcg tagaaccagg
    11281 tacagaagta tatgaaggaa tgattatcgg ggaaaacagc cgtgataatg atttgactgt
    11341 caacatcacg aaagcaaaac aaatgacaaa cgtacggtct gctactaaag accaaacatc
    11401 tgtcatcaaa aaaccaaaac aattgacgtt ggaagaatct ttggaattct tgaatgatga
    11461 tgaatattgc gaagtaacac cagaaagcat tcgcttgaga aaacaaattt tagagaaaaa
    11521 tgctcgtgaa aaagcatcta aaaagaaaaa ataatctgta aaaggatgaa gagagaaacc
    11581 agcttgttat aaaaaatagc tggtttcttt ttttgtccaa ctcttttctg tggagaagtc
    11641 atctttgtca atggacataa attcagaaaa tacgccgaaa gacgtttaaa agacttttaa
    11701 aaaacttatt tctagagagt ttttgtttca taataagata atgtttcacg aaaggagatc
    11761 aacttgtcct tcgaagaaaa tcgaagtaaa atggcaagag aaaggagcgg gaagatgaac
    11821 gacttattaa aacaatactt tggctatgat gagtttagac ctggtcaaaa agaaattatc
    11881 cagaaagtca ttgatcagga aaatgtatta ggaatcatgc ctaccggaag tgggaaatca
    11941 atctgttacc agcttccagc tttactatta gatgggctta ctgtggttgt gtccccttta
    12001 atctctttga tgaaggatca ggtggacgcg gcgaatcaat taggtatacc cgccactttt
    12061 atcaatagct cgttagatgg ttacgaaaca gctagacgct tccaagaaat cgatagacag
    12121 cagtaccgtt tactttatat cgctcctgaa cgttttatca tgccggattt tatccaagca
    12181 atgaagcgat ggaacgtccg tatgatcgct attgacgagg cacattgtat ctctcagtgg
    12241 gggcatgatt tccgcccaag ttatttacag atggcgaacc agctagatca actgccaaat
    12301 cgtcctgtaa ttgttgcttt gacagctaca gcgactgtgc aagtagctgc tgacataaaa
    12361 agacttttaa aaattccaga aaacaaccat attcagactg gttttgaacg agaaaatcta
    12421 cgatttcaag taattaaaga tcaaaaaaaa gaacaatatc taatcgaata tttaaagatc
    12481 aacaaaaatc aatctggtat catttatgca gcaacgagaa aagaagtaga ccgtctctat
    12541 catttgctta aaaaattcga tttttccgtg ggtagatatc atggtggtct aaatgagaat
    12601 gagcgaacag aaatgcaaga agccttttta tacgatcgct tgcagctgat tgtagcgaca
    12661 aatgcatttg gtatgggtat aaataaaagc aatgtccgtt ttgttatcca ttatcaaatt
    12721 cctggctctc tagaagcata ctatcaggaa gctggtcggg cgggaagaga tggtctttcc
    12781 agtgaagcca tcctgctttt ctctcctcaa gatatccaag tccaaaagtt ttttatccag
    12841 cagtcccaaa gagaagaagg gcaaaagcaa aaagaatatg aaaaattaaa agcgatgaca
    12901 gaatacgtgt atattgagtc ctgtcttcag caatacatcc taaactattt tggcgagaca
    12961 agcagtccgt gcaaccgttg tggtaactgt ttagatgaca gagaaatagt agaagtaacg
    13021 acagaagcac agatggttct ttcttgctta aaacgtatgg gtgagaacta tggtaaacaa
    13081 atgctgatga aagtcttagc aggatcgaaa gaacaaaagc tgcaagcttt aggttttgga
    13141 catttatcta cctatggact gatgaaaaac caatcgcaaa aagaaacgat gcagttaatt
    13201 gaatatttga tatcgaatgg ctatcttctg accatcaatg gtgagtatcc tgttttgaaa
    13261 gtaacagaaa gagggataca agtattgaaa ggacaagaat ctgtttatcg aaaggaaccc
    13321 aagaaagtcc agcagctatc ggatgaagaa accgatacac tatttgaagt gctgcgggaa
    13381 ctgcgaacgg atctagcatc agaagcaggc gttcccccgt atgtcgtatt ttcagattcc
    13441 acattgaaag aaatgagccg aatacgtcca tcaagcagat tggagatgct tcagataaaa
    13501 ggagttggcc aaagcaagct tgataaatat ggcgaagcct ttttatctcg gataaaaaat
    13561 gcggactcta acgttctgga taagtagaaa gaacgaatac gtaaaaatga ataaaaacaa
    13621 ctggttaaat ctgttttttt tgctccttat gaaaatcaaa tatgttataa ttaaggaatt
    13681 agaatgggag gtaagcacag atggattctt tgtcgaaaaa acttgcgatc caagtattaa
    13741 atgatgaagc agaacggatc aaacgattaa tccgaaatca aaacaacagt ctctgtatct
    13801 cacaatgcaa agccttcgaa gaagtggtag atacacagat gtacggcttt tcccggcaag
    13861 tcggttttgc tgtccgtata ggagtaatcg gaaagaacga gggccagctg atgttaagtg
    13921 agctagaacg agaattgaat cagttatata gtgaggttta ccaagaaatt tatgataaaa
    13981 aagaagttgg caaggaggaa tagacttgcc taagaaagtg aaaaaaaggc atctattaga
    14041 ctatagcatc ttgattccct atttaatttt atgtgtattg ggattgatca tggtatatag
    14101 ttctacgtcg tatctattat tagaaaacgg acaaaatccg tccgcatcag tcattaacca
    14161 gagtattttt tgggtgctta gcttgattgc tatcgcacta ctttataaaa tgaaaacaga
    14221 tgttttgaaa aaccagcgtt tgatcatggc agccatcgca gttttgacga ttttgctgct
    14281 gattgttgtg ttttttggta aggagatcaa tggggcgaaa ggttggctgc aaattgcagg
    14341 attctcgatc caacctgccg aatacctaaa aatcatttct atctggtacc tgtctctgac
    14401 tttatctaaa agacaaaaca gtgtacaaaa agattttctt gggactgtga aacgtccact
    14461 tgcaatggtt atcggactta ctgcattagt cgctattctt cccgattttg gtaatgcggc
    14521 agttatcttt ttgattattt tggttctgct tttagccagc ggagtcaatt atgtgtatac
    14581 tttgattgta ggagtaggag gcttttgtct aagtactttt acgatctggc tgatcaatat
    14641 cacaaacgga aagatcttac ccggacgttt acagtatatc tataatcgat ttgctattta
    14701 ccaaaatcct ttctcagacg aattaaataa agggcatcaa ctagtcaacg gctactatgc
    14761 catgttcaac ggaggactat ttggcagggg attaggtaac agtatccaga aaaaaggatt
    14821 tttacaagaa gcacaaaccg attttattta tgcaatcgta gtggaagaac tgggagtgat
    14881 tatggggatt ttgatattag cgttgctatt ctttatgatt gtgcgcattg tccttgtagg
    14941 tatccgttca aaagatccgt tcaattcgct tttgtgcatt gggatcggcg caatgttttt
    15001 gatccaagta tttgtcaatc ttggaggaat tactggcgtt attcctttga cagggatcac
    15061 gttcccattt ttaagtcaag gaggctccag tttgctgatg ctttcgattt gtgtcggctt
    15121 tgtcctcaac atcagtgcgg atgaaaaacg caagagctta ggtttataat aaaaaaagat
    15181 gtaggagtgg ttcgatgaaa aaagttttag ttgctaacag gggtgaaatc gctgtacgtg
    15241 tatttcgtgc ttgtacagaa ttaggaatta aaactgttgg aatctatgca gaagaagatg
    15301 aatattccgt acaccgtttc aaagcggatg aagcatattt agtaggacaa ggaaaaaaac
    15361 cgattgatgc gtatctagat attgaaggga tcatttctat cgcgaaagaa tgcggagctg
    15421 acgctattca tccgggttac ggtcttttgt cggaaaactt gaattttgcg caacggtgcg
    15481 aagaagaagg catcattttt gttggaccca aactgcatca tttagatatt tttggagata
    15541 aaatcaaagc aaaagctgca gctatcgaag cggggattgc ttcgattcca ggcacagatg
    15601 gaccaatcgc ttctatagac gatgccttgg agtttgcgaa acaatacggt tatccgatta
    15661 tgatcaaggc agctctaggc ggcggcggac gcggcatgcg tgtagctcac gatgaaaaga
    15721 gtgcaagaga gggctacgaa cgtgcgaaaa gcgaagcaaa agcagccttt ggtagtgatg
    15781 aagtgtatgt tgaaaaatac atcgctaatc ctaagcatat cgaagtacag attctcgggg
    15841 atacgcatgg aaacgtcatc catctttttg aaagagattg ttctgttcag cgccgtcacc
    15901 aaaaagtagt agaagtagcg ccttgtgtct ctatgaatga acagcaacgt caaaaaatct
    15961 gccaggcagc tgtccaattg atgaaatatg tcgggtatgt caacgcaggt acagtagaat
    16021 ttcttgttga aggagatgat ttttacttta tcgaagtcaa ccctcgcgtc caagtggaac
    16081 atacgatcac agaaatgatt acagatattg atatcgtaac aacgcaactg ttgattgctc
    16141 aaggtttgga tctgcataaa gaaatcggat tgcctcagca agaaggaatt aaattaaatg
    16201 gttcagccat tcaatgccga atcactacag aagacccgtt gaataacttt ttaccagaca
    16261 cagggaaaat cgatacgtat cgttcacctg gtggttttgg cgtacgtctg gatgtcggaa
    16321 atgcttacgc gggctatgta gtgacaccgt attttgattc attgctagta aaagtatgta
    16381 cgcatggtgc tacctttgaa acagccattc agaaaatgga acgttgcttg aaagaattta
    16441 gaatccgtgg agtgaaaaca aacattccat ttatgctcaa tgtcatcaca catcctgaat
    16501 tccaatcagg aaatgcaaaa acaaccttca ttgatagtac agcaacatta tttgaatttc
    16561 ctcgactacg agaccgcgga aacaaaacga tgaaatatat cggtgaaatt actgtgaatg
    16621 gttttccagg tatcgaaagt ggcgaaaaac cattttatga agaaccacgt atgcctaaag
    16681 atctaatcac aagaactgat tacgtaacgg ctaagaatgt attagatgcg aagggtgccg
    16741 atgctttggt cgaatggatc aaaggacagg aaaatttgtt gttgacggat acgacattta
    16801 gagacgccca tcaaagtctg ctagcaactc gtgttcggac aaaggatttc aagcagattg
    16861 cacgtttgac tggagaaggt cttcctgaac tcttttcaag tgagatgtgg gggggagcaa
    16921 catttgatgt ggcttaccgc tttttgaatg aagatccttg gcaacggttg agaaaaattc
    16981 gttctttaat gcctaatacg ttgctgcaaa tgcttttcag aggttcaaat gccgttgggt
    17041 attctaatta tcctgacaat gtattggtgg aatttgtcaa agaagcagca gcgcaaggaa
    17101 tcgatgtatt tcgaatcttt gatagtttga actggactcc acaaatggaa aaaagtatcc
    17161 aagctgtacg ggatactggt aaaatcgcag aggcagctat ttgctatact ggcgatatca
    17221 acgatccaag tcgggcaaaa tacaatgttc agtattataa agacatggca aaggaactag
    17281 aacaactggg tgcgcatatc attgcaatca aagatatggc tgggctgctt aaaccgcagg
    17341 cagcttatcg tttgattagt gaattgaaag aaacgacaga tctgccaatc catctgcata
    17401 ctcatgatac cagcgggaat ggaatcatca cttattctgc tgcttcaaaa gcaggagtag
    17461 acatcgttga cgtggcaatg agtgcaatga gtgggaatac tagtcagcct agcatgagca
    17521 gcttgtatta tgcactggtc aatggtccac ggctgccaga aatcacgatt gaaaatgcac
    17581 agaaattaaa ccattattgg gaagatgtgc gcatgtatta caaaccgttt gaaaacggct
    17641 tgaatgctcc tgaaacagaa gtatatatgc atgaaatgcc aggaggacag tattcgaatc
    17701 tacagcaaca ggcaaaagct gtcggattag gtcatcgctg ggatgaaatc aaacaaatgt
    17761 atcatactgt caacttgatg tttggcgata tcgttaaagt aactccatca tctaaagtag
    17821 ttggagatat ggcactattt atggtccaaa atgatcttac agaagaagat atttatgaaa
    17881 aaggcgaaac actaagtttc cctgaatctg ttgtgacttt cttccaagga gaactagggc
    17941 aacccgttgg cggatttcct aaaaaattgc aaaagattat tcttaaaggc agacctgcat
    18001 tgaatgaacg tccaggtctt ttggctgaat cagtcgactt caacgaagta aaaaaagaat
    18061 tggcagaaaa aattggctat gaaccaaaac aagacgaagt attaagctat ttgatgtatc
    18121 cgcaagtctt tttggactat caaaaagcat ataatcaatt tggcgatgtt acattactgg
    18181 acacaccaac atttttccaa ggtatacgtt tgggtgaaac aatcaatgta caaatcgaac
    18241 gcggaaaaac gcttatcatt cgtttagatg aaatcggaga acccgatatc gaagggaatc
    18301 gcgtactgtt cttcaatttg aatggacaaa gacgtgaaat cactatcaaa gataattcga
    18361 tcatcagtac ggtccaaacc cgattgaaag ctgaaccgac aaaccgagaa caaattggag
    18421 caacgatgtc tggttctgtt ttggatgtat tagtgaaaaa aggcgataac gttaaaaaag
    18481 gtgatacgtt gatgattact gaagcgatga aaatggaaac ggccatcgaa gcacgttttg
    18541 acggagaagt cgcacatgta tatgtgtctt caggagacac tatttcgtct ggtgaccttt
    18601 tgattgaagt gactgagaaa taataagcga aaggtgggga aacatgaaaa gactgggttt
    18661 gttttggctg attctgctat tagttattgt ggcattctac atggaaccag tcttgtttcc
    18721 gccaaaactt gaagtgaagg aaaactctga tcaacaaata tcaaaccaaa tgacaacgtg
    18781 gaaataccag gagcttaaag caactggttt ttctacttat atcggaaaac cgatcgatca
    18841 gctggaaaag gattttggcc aagcctatga tcgattaact agcggatttg gttttgaaac
    18901 aagatattat gaaaataaag ctagtcatct cacatttgaa gcgaacatcc aagacagcag
    18961 agtcacaact gttaaagtac tgcaaacagg gcctgctgat atcgcgccgt tttatttagg
    19021 gatgacgatg caagatctta cgaaaatcac tactatctat actaatttca cttttgagta
    19081 ccatgataca gaagttggaa tcgaactaat ggaagaggat atgaattatc gtccgctaat
    19141 tgcttttgat aacgacactt ttgctatttt attttttgat caaacaacaa acggcctatt
    19201 ttcagttgtg tatttagata aagatagtct tttgaaacta ttgccatatc aagtgtttgg
    19261 tgaaggcctt ccacattatc aagcagataa atcggctgat tgggaatcaa tcaatcgcgt
    19321 taaagaacga aaaagtacga ctttattaaa tatgcttaga agagaagacg aacttcccat
    19381 atacaatcaa acacttgcct ttcaaaacca cagtcagctg ctactacatg attttttgac
    19441 aaggcctgag gaaatattgt cagaggaacg tatggctgat tggcaaaaat cgctaggcag
    19501 tgctcaaacg acagtgaaat tctcactgac gaatgatgaa ttggagaagt taatcagtaa
    19561 acaaaaatta gaaaatacta gtgggctatt tattcatccg gcgattgacg ctactttttc
    19621 gtttttatat tggtacagcg atccttacaa ccatgaccgt ttcatggacc cgggaacgga
    19681 ttctgtaggc attgcttttt ctaaagaaaa tatgttagtc ttattgcaag aagaaaacgg
    19741 agaaagtagt gatagcagtg atcgttaacg aagagctgtt tcaactggag gatcaatgcc
    19801 tccaagtcgc tgaaatgata aaaaagagta aaacgatggc ttcttatctt gaacataaac
    19861 agtcaatgga agaatctgca aaagtttgcc agttgaaaga agcgttcttg tctaagaaag
    19921 aagcatttga aaatgtagcg gcatatggtg agtatgcacc tgattatcgt gaaaaaaaac
    19981 gagctgttcg aaaagcaaaa agacagcttg atatggatga gcatgttgca gctttccgat
    20041 atagtgaaac acagctgcaa agtctgttgg atcaaattgt ccaagtactt gccgaagaag
    20101 tttctaatga aatcaaagta gatgcaggta gcccgttttt tgaaacgaaa aaacactttg
    20161 gatgcggagg taattgtcat gcaattagac aatagtgaat ttgaaataac aaaacgacgc
    20221 gggctgatcg tatgggtcta cagcttaaaa caattaaaga atctgaaaaa gttcggcttg
    20281 ttgcattatg tctcaagaag aatgaaatat gtcgtgcttt acttaaatga agagacattg
    20341 gaagaaacag aagaacgaat ccgtaaatta cattttgttc gaaacgtaga acgttcttac
    20401 cgaccggata tcgaaatgaa ttttgccgaa aaaataggaa acaaaacagt attaaaagac
    20461 gaaggtttcg aaattgaaga gctaaataca gaaatccgat tagctgaaca tctttaaaaa
    20521 tctcttatga gcagttttta aataaaaaat aacttggata acgatcgatt tttgatcatt
    20581 gtccaagtat ttttttggta tactagtacc agtaccattt ctaaggagtg agtagatgcg
    20641 gataatatca ggaaattatg gcgggcgccg tttaaaaagt ttagcagggg cgaacaccag
    20701 accaactaca gataaagtaa aagaatccat attcaatatg attggaccat attttgatgg
    20761 agagacagta ttggatctgt ttgcaggaag cggtggttta gctatcgaag cagtatctag
    20821 aggctgcagt catgcggtat gcgtagacaa aaattaccaa gcgctaaaaa tcatcaaaga
    20881 aaacatcgaa attacgaaag aaccagagaa atttaccaca ataaaacttg atgcggacaa
    20941 agcaatcgaa cggctagcag cggagaaaca acaattcgac tacttgtttc ttgatccgcc
    21001 atatgcaaaa caaaaaataa tcgatcaaat ccataaaatg gaagaactgg gacttttttc
    21061 taaaaccgca gttatcgtat gcgaaacgga taaaacagtc gaattgccag aaactgtggc
    21121 tggattccat caaataagaa aacagattta tggcataacg gccgtaacta tctatagaaa
    21181 agagggaaat caatgaaacg tgcattattc ccaggaagtt ttgatccttt tacaaaaggt
    21241 cacttagata cggtggaacg tgcggctaaa ttattcgatg aggtagtaat tggtgttttt
    21301 atcaatactt caaagaaaag tttgtttccc ccagaagaaa gaatgacatt gatcacaaaa
    21361 gctgtttctc atcttcctaa tgtaaaagtc atgcatcaag aaaatcaatt gacggtagaa
    21421 acagcaaaag agattggtgc agatgcgttg atcagaggca tacgaagtat caaagacttt
    21481 gaatatgaac gagagattgc tcagatgaat catcatctat atgatgaatt ggaaactgtc
    21541 ttcttattag caaaaccaga atatagtcac gtgagttcca gtattttaaa agaagtactg
    21601 cactttggcg gagatgtgag cagctactta ccgccagtga tcaacgaagc tttagctgga
    21661 aagcgtgaat caaatgaaag ctaaacaatg gttgaaaaca gtactgatta tcatatttgg
    21721 aatgttgatc attggatttg tagtagttcc cttgccgtat tatgtagaat caccgggagc
    21781 cacgatcgat ctgaaagaac taattacggt caatgataag gaagataaag gttctgggtc
    21841 tttctctctt acctctgttg gtatcaggca agctacaggt ttcttagctt taaaagctaa
    21901 aatgacccct tttgaagaat tgatcagtga agaagaattg actggtgggg cgacaaatga
    21961 agaatataat caaatgcaac agtattatat ggattcttcc caaaatgcag caatcgaaca
    22021 agcgttaaaa cttgcagaca agccttataa aatggaattc aaaggcgtat atgtcatgag
    22081 tgtagagaaa acatcgaatt tctttggcaa gctttcagtt ggagatacag taacaaaagt
    22141 agacgggaaa agctttcaaa gtacagaagc atttatggat tatgtaaaaa gccaaaaagt
    22201 tggacaaaca atcgaaatca gttatttgca taatggtgaa gaaaaaacag cttctggtga
    22261 tctgatcgag ttaccaacag ataaaaaggc aggaataggt atcacgctta ctgaccatac
    22321 cgagatcgaa tcagatacag atgttcgtat taattctggc tctatcggtg ggccatcagc
    22381 gggattgatg ttcactttag aaatctatga acaagtcact ggtaaaaact tgcgacatgg
    22441 taagcaaatt gcaggaactg gaacgatcaa cagtgaagga gaagttggcc gaatcggagg
    22501 aatcgataaa aaagtggcaa gtgcggataa agctggcgta gagattttct ttgcaccaga
    22561 tgatacgatt tcagacgatg ttaagaaaga atatcctgaa ataaaaacca actacgaaga
    22621 agcaaaagct gccgctaaaa aattgaattc ctcaatgaaa atcgtaccag taaaaacagt
    22681 acaagaagcg cttgactatc tagagaaaat gaaatgaaac taaataatta aagaggggt
//
LOCUS       DAZAID010000046        22497 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000046 DAZAID010000000
VERSION     DAZAID010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.046"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..424)
                     /locus_tag="ACG9BE_001591"
     CDS             complement(<1..424)
                     /locus_tag="ACG9BE_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFN3032336.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPH"
     gene            complement(437..847)
                     /locus_tag="ACG9BE_001592"
     CDS             complement(437..847)
                     /locus_tag="ACG9BE_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3032337.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1122..1334
                     /locus_tag="ACG9BE_001593"
     CDS             1122..1334
                     /locus_tag="ACG9BE_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFN3032338.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1337..1549
                     /locus_tag="ACG9BE_001594"
     CDS             1337..1549
                     /locus_tag="ACG9BE_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032339.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1689..1844
                     /locus_tag="ACG9BE_001595"
     CDS             1689..1844
                     /locus_tag="ACG9BE_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032340.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(1841..2065)
                     /locus_tag="ACG9BE_001596"
     CDS             complement(1841..2065)
                     /locus_tag="ACG9BE_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFN3032341.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2143..2331
                     /locus_tag="ACG9BE_001597"
     CDS             2143..2331
                     /locus_tag="ACG9BE_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032342.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2470..2598
                     /locus_tag="ACG9BE_001598"
     CDS             2470..2598
                     /locus_tag="ACG9BE_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032343.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2657..2992
                     /locus_tag="ACG9BE_001599"
     CDS             2657..2992
                     /locus_tag="ACG9BE_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032344.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3225..4166
                     /locus_tag="ACG9BE_001600"
     CDS             3225..4166
                     /locus_tag="ACG9BE_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFN3032345.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4168..5058
                     /locus_tag="ACG9BE_001601"
     CDS             4168..5058
                     /locus_tag="ACG9BE_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFN3032346.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5101..5901
                     /locus_tag="ACG9BE_001602"
     CDS             5101..5901
                     /locus_tag="ACG9BE_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFN3032347.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            5916..6767
                     /locus_tag="ACG9BE_001603"
     CDS             5916..6767
                     /locus_tag="ACG9BE_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3032348.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            6833..6967
                     /locus_tag="ACG9BE_001604"
     CDS             6833..6967
                     /locus_tag="ACG9BE_001604"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032349.1"
                     /translation="MDELSQQESRMVRKISKLIIYRDTERFLTVLGNVYENPELLEVE
                     "
     gene            6968..7429
                     /locus_tag="ACG9BE_001605"
     CDS             6968..7429
                     /locus_tag="ACG9BE_001605"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3032350.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7455..7907
                     /locus_tag="ACG9BE_001606"
     CDS             7455..7907
                     /locus_tag="ACG9BE_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032351.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8013..8207
                     /locus_tag="ACG9BE_001607"
     CDS             8013..8207
                     /locus_tag="ACG9BE_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032352.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8238..8624
                     /locus_tag="ACG9BE_001608"
     CDS             8238..8624
                     /locus_tag="ACG9BE_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032353.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            8621..8824
                     /locus_tag="ACG9BE_001609"
     CDS             8621..8824
                     /locus_tag="ACG9BE_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFN3032354.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            8821..9117
                     /locus_tag="ACG9BE_001610"
     CDS             8821..9117
                     /locus_tag="ACG9BE_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032355.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            9193..9609
                     /locus_tag="ACG9BE_001611"
     CDS             9193..9609
                     /locus_tag="ACG9BE_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFN3032356.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            9870..10007
                     /locus_tag="ACG9BE_001612"
     CDS             9870..10007
                     /locus_tag="ACG9BE_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032357.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            10417..10647
                     /locus_tag="ACG9BE_001613"
     CDS             10417..10647
                     /locus_tag="ACG9BE_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032358.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            10806..11639
                     /gene="terS"
                     /locus_tag="ACG9BE_001614"
     CDS             10806..11639
                     /gene="terS"
                     /locus_tag="ACG9BE_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFN3032359.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11632..13047
                     /locus_tag="ACG9BE_001615"
     CDS             11632..13047
                     /locus_tag="ACG9BE_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFN3032360.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13059..14588
                     /locus_tag="ACG9BE_001616"
     CDS             13059..14588
                     /locus_tag="ACG9BE_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN3032361.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14506..15558
                     /locus_tag="ACG9BE_001617"
     CDS             14506..15558
                     /locus_tag="ACG9BE_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFN3032362.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15560..15877
                     /locus_tag="ACG9BE_001618"
     CDS             15560..15877
                     /locus_tag="ACG9BE_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032363.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16004..16627
                     /locus_tag="ACG9BE_001619"
     CDS             16004..16627
                     /locus_tag="ACG9BE_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFN3032364.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16642..17532
                     /locus_tag="ACG9BE_001620"
     CDS             16642..17532
                     /locus_tag="ACG9BE_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFN3032365.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17555..17770
                     /locus_tag="ACG9BE_001621"
     CDS             17555..17770
                     /locus_tag="ACG9BE_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032366.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17782..18123
                     /locus_tag="ACG9BE_001622"
     CDS             17782..18123
                     /locus_tag="ACG9BE_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032367.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18123..18494
                     /locus_tag="ACG9BE_001623"
     CDS             18123..18494
                     /locus_tag="ACG9BE_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032368.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18487..18882
                     /locus_tag="ACG9BE_001624"
     CDS             18487..18882
                     /locus_tag="ACG9BE_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFN3032369.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            18884..19261
                     /locus_tag="ACG9BE_001625"
     CDS             18884..19261
                     /locus_tag="ACG9BE_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFN3032370.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19262..19870
                     /locus_tag="ACG9BE_001626"
     CDS             19262..19870
                     /locus_tag="ACG9BE_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFN3032371.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            19870..20211
                     /locus_tag="ACG9BE_001627"
     CDS             19870..20211
                     /locus_tag="ACG9BE_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032372.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20453..22497
                     /locus_tag="ACG9BE_001628"
                     /pseudo
     CDS             20453..22497
                     /locus_tag="ACG9BE_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT         8143 a         3275 c         4652 g         6427 t
ORIGIN      
        1 tatgaggaag ccctaaatat tcaataattt ggaactgact catatcagat aaatcttgtt
       61 catctattgt tctagttaac atttcaattg caaacatatt agcttcagct tctataccat
      121 aaatcataca actaaggttg tttgctctaa aagctgctgt gcaaatacct ttatggagtc
      181 taacgtgggc tatttcatgc cataatacaa atcttttcat agaaggtgat aggttttgat
      241 taattaatat cacataacat cttttattgt gtatcgtttg tcctaatgtt ttattcccta
      301 aatcagtttc aattaaatct atatcagaat atttgattat tttatatgga ttaatcgcat
      361 cagatccaaa ctctacaaat aatttttcta tttcttctaa tatccagtat ttgtttaaat
      421 gcataccaaa gcccacctat tgacgatatt ttttaggagt gaattttttc tttgctttaa
      481 ctttggttaa ggcaagtgca agttgaatgg catcgtataa tttttcctta tcttcatcgt
      541 ctaaaggttc accataataa tttgtttcag catctgaatt caagccttca agtaattttt
      601 cagcttgtaa agcaatatct tgtttatctt tttctgttaa atcataataa tggcgtttat
      661 cagtacgtcc taaaagataa tcagttgata cattaaagta atctgcaact ttttgtattt
      721 tatctccaga tggagtactt ttattccatc tagacatgct tccatttccg aaatttaact
      781 ttctttcaag ttctgccaac gtcattgaac gttcttttaa taagattttt atacggtcta
      841 atagcatagg atatgcattc ctttcaaaat accgacaaaa aaagtatttt tctgcaaatt
      901 atctatttac aaatagagaa tttgcagtta tactagtttt gtaagcaaga tatttacggc
      961 atacaaagac taaaagcaat aaactaatag tctccccaga catcagtaag ctcgcttttt
     1021 gtgcgctgta ttttgtatgc ttacataata gcatatttgc agtttttgtc aaacttatta
     1081 gacaaaaata ttagaaaata ttcgataagg gggtatgaag catgacttta aaacagcaag
     1141 tattaattgc tttagtaaag aaaggttgga gtcaaagaga gttagctcga cgaatgggta
     1201 tatcaattac ttatttacga gatatcttca tcgaaaaaag aaaacctaag gaacgtttaa
     1261 aacaaatcga agaacttttg gatatcaagt tagaagtaga ttctaatgat cagccatcag
     1321 aacaggaggc gtaaaaatgg tacaacgttt gagcaaagcg gagtttaaag agaaatataa
     1381 gtattctgaa agtacttatc aaagaagaat gaaggaattt aaaaaatcac gttttagtga
     1441 ggggtataac gcagttactt ccaaagaaat aacaattgat gtcgatttat atgaacagtt
     1501 tagaacatgg cgttctaaaa atcgatttag agttagagct gtttgttaat agctaaaaaa
     1561 tctaaattca ccacttctga aaagaggagt tgctgctctc ctttcaagtt tgccaaagtc
     1621 cacgtttttt cattttggca actcctcttc ttggaggtgg tgacggcgag agacgaagga
     1681 gggatgacat gaaggaagaa tcaaaaatag ggattttgaa tgagaacaat tacacaagat
     1741 taattattga aacagaagaa gggaaaaaag tagcagaagt tacattgacc gatgcaactc
     1801 ctgctgacgg ttatcgtatt cgattaactc ctaaatatga ttaacctttt ggtgggtgtg
     1861 gatcgtttcc gtgactatca cggctttgaa tttttccatc tctgccatga ataaatagct
     1921 cagatccttg atttttagaa atctctctag cgatttttac tgcttctgat tttgtattag
     1981 tatgtgcagt cgctttagaa tttccagcac ctttgacatt ccaaccacca ttggaattag
     2041 gtacaacatg ttgatttttt cccatagttt tcacctccat ttctatagaa atattttatc
     2101 aaatagaaat ggagaaattc aataaacgaa aggagaagtg atatggcata taccttgcaa
     2161 caagaacatc aaattctcgg tttgattaaa caacgcagga aacaattaca agatgaccgt
     2221 gcagcgctta gaaaagccga tgagctatca gataggcaag ctgaactaat tgcttctgaa
     2281 cttgaggatt tgagaatgct agaaataaaa aatagggaga ttagattatg aagaagacag
     2341 acacactttt tataggattc attttggggt tattagtgat tgtagcgcac caaagtatta
     2401 tcgggggaag cttgttcgca gcattgatgg ttttaatcaa tctgcttgat tcaaaagaaa
     2461 ggagcaacta tggcacgaga agaagcgcta aaaatcggta aagtgattgc tgataatcgg
     2521 tggggaaata gccgccctat tattttaagc aagcaacata tcgaaaagca aaaagcatgg
     2581 caacaaataa aaaagtgact ctgccagcca gcaaagagtc acatacaaat ttataaaact
     2641 aggagaatta tatcacatga ataagaaaat tgaaaatctt atcgaagaat tgaaacgtga
     2701 atgccaaaag caaggtgttt ctattatttg tacagcccaa aaagaagggg aacttaaaag
     2761 tctcgtttat ggtgagacaa cagaaatttt actttgtctt gctatgcaag aagaacattt
     2821 agatgaaaat ctcccattgt cagcacatat tatgcgtaga attgctgtgg atgcttacga
     2881 acaagcgaaa aatgaagaag aaaatcaacc ttcaaatcat acttttgtta ttaataacaa
     2941 ggaagattta gctgatgtga tgactcgtat tctcaagggg gaatttcaat gatgaatgaa
     3001 aaaacaattg atctactcat tattttattt ttcacgttag tgattgtagc gaaaagttat
     3061 gtaatctttg gcttgttatt cgcaattacc atgcttgtgc tttgcgtgtt agcaagcaga
     3121 aaagaggtta aacatgaacg agttagcaaa tcttgacaac tatttaactg atcctgatta
     3181 tgctaaacca ccatatgaag caccaattga tgaggaggat gaagatgagt aaatctacct
     3241 tagaaatgag ccatcaagaa tggcttgaag accgtaagaa aggcatcgga ggttcggatg
     3301 ttggaacaat tttaggattg aataaatgga aatctcctta tcaactatgg ctagaaaaaa
     3361 caggacaagt agtacttgaa gaatcaggaa gtgagcccgc ttattggggt aatgttttag
     3421 aagaagtggt tgctaaagag tttcaggaac gtacaggcaa aaaagtacgc agaagaaacc
     3481 aagtatttga acatccatta catccatttt taagagcaaa tattgatcgg gaagtagtgg
     3541 gagaaaatgc cattctggaa tgcaaaacag ctaatcaatt tctcggtaaa gaatgggaag
     3601 gcgaagaggt accgcttagc tatctctgtc aagttcaaca ttacatgaac gttctaaaca
     3661 aagactattg ttacatcgct gtcttaatcg gtggtcaaaa atttatctgg aagcggattg
     3721 aacgagatca agagctgatc gatacaatca ctgaacaatt agtagagttt tgggaaacaa
     3781 acgttcttgg aggtatcgag ccaattattg atggtagtca agcgacagct gatttcttga
     3841 aagagaagta tgcagatgtt gaagacgttc aaacgacgtt accaattcat ttcgatgaac
     3901 tagttgaaca gaaaaacgaa ctcaaacgga ctaagaaaga aattgaatca gctatccgtc
     3961 aagtagacaa cgagattatc agcgagctag gtaaacgtga agccagtatc ggtatcactc
     4021 aaaaaaacat catcagttgg aaatttgtcc gtacgagacg tatgaactcg aagaaactag
     4081 cagagaaata tccagatgtc gcaaatgatg aagagattta taacgttact gaatcaagaa
     4141 ggctaaccga aagggagatc aaataatatg gcaacaaatg aatcgttaaa aaatcaattg
     4201 gcagcaaagc cacagaaaca agttgcacca ggtcaattag gacttaaagc tttgatgaat
     4261 acaccaacaa tgagaaagaa gttcgaagag gtgcttcatg acaatgctaa tgcttttatg
     4321 tcgaatgtta tgactcttgt atctaatgac agttatcttg cagatagtga accgatgtct
     4381 atcatgagtg gtgcgttaac tgctgcaaca ttaaatcttg ggctagataa gaatttaggt
     4441 tatgcatatt tagttccatt caatagtaaa aacaagcaaa caggaaaatg ggaaaagaaa
     4501 gctcaattca tgcttggcta taaaggatat atccaattag cccaacgatc aggtaaatac
     4561 aaagcattaa atgtgatcga agtttacgaa ggagaactaa aaagctggaa ccgactgaca
     4621 gaagagtttg agtttgatcc aaatggtaga acgtctgatg aagtcattgg atatgttggc
     4681 tatttcgagt tactgaatgg attcaaaaaa actgtctatt ggaccaaaca agaaatcgaa
     4741 gctcatcgga ttgctaacaa taaagatcgc gataagacaa agttaagtgg tgtgtgggca
     4801 tctgattaca atgcaatggc acgaaaaact gttttgagaa atcttctttc taaatgggga
     4861 atcttgtcca tcgaaatgca agaagccacc acatcggatg agagagtcca aagggttcaa
     4921 gaagacggca gcattattgc tgaaacagaa gttgaagaag atattcctga aagaaaagaa
     4981 gcagaggtta tatctgaaga aaatgaagat gtacaaactg gattatttga tgcatctaat
     5041 ccgccgttaa acaaataatg agggagcttt ctcccttgct tttctagagg agaaatacga
     5101 atgaacacag gatatgtcaa attacatcga aaagtgatga attcattcgt ttggaccaat
     5161 ccttatatgt ataaattatg gaacttgtgt ttgatgaaag cgagtcatga gaatcgcaag
     5221 atacttttta atggaaaaga gatacaagtg aacagcggag aattcgtcac agggcgcgat
     5281 gctatcacat ctgagatgaa cgaaggcgtg aaacgtgaac atcaagtgaa cagcggttct
     5341 gtatggagat ggctaaaaca atttgaaaaa aacggaatgt tgaacatcaa atcaactacg
     5401 aaatacagcg ttatatcaat aaaaaactgg tctttgtacc aaagcagtga acaacaaatg
     5461 aacatcaaac gcacaacaag tgaacaacaa gtgcacacaa acaagaatga aaagaattat
     5521 aagaatgaaa agaataataa aacatcatcg tcgcaaccac gcaaaaagcg tgtttacgac
     5581 accgactcag tttactacat tctcgcggag gagttattca aacagatttg tcagaatcag
     5641 gaaatcaaaa agccgaatct gcaaagttgg gcagataaca ttcggaaaat gatcgagatc
     5701 gacaagcgga ccgaaaacca ggtacgggga atgattgaat ggagtcagca caatgtgttt
     5761 tgggcatcga atattttatc tgctaaaaaa ttgcgagaaa aatacgacac aatggcagcg
     5821 caagccaatc gtgattataa aacaaaacaa actaaaacgc ttgaatacga gaaatttagc
     5881 acagatgagt tgcctatttg agaggaggcg taggcatgga aactgttggg gaaatcatgg
     5941 aaaagctgat acagaaagtg cttgtccaac gtggcgaatg tcctgaatgc ggacagcctt
     6001 tgtatggatg gcgtacgaag aaccctgatg gctcagaacg ttgtaagcca acctgtatgc
     6061 aatgtgggta taaagcattg cgagttcagg aagacttaca aacagaacga atttacaacg
     6121 agagcctgaa agcaagagca atcaattttt tcaaaggtgg ttctgttgtg cctaatcaag
     6181 cgttgtttga ttgcacattg cagaattatc aaattgtcga tcaagaaaca agacaagcgg
     6241 ttgaagtaac caaacgcttt gttaattcag tcttgttagg aaatccaagt caccttgttt
     6301 taactggaaa acaaggaaca ggtaaaagtc acctagcgat ggcagcggct tgggaagtct
     6361 tgaagcgatc aaactacgat aaaaaaatct tatttatcgg gttacaggaa atgctggatc
     6421 aaatcaaatt ttcgtacaac aatcctgaac tcagaaaaac gattgaggga tcgttgattg
     6481 cagatatcaa aacagcggat ttagtcatca ttgatgatat cggttcagaa ctaggaaaag
     6541 atgcatcaga tagtcgagcg tttggcataa acacgctaaa ttcgttcttg gatgcacgac
     6601 agaacctagc aacgattatc acaacgaact tgcttggtga agaactgaaa aaagcttacg
     6661 gtacgagaac aatatcaaga atgtttgtca actctgatgg atttacgatg gtattttctc
     6721 aaacagcaga caagcgcata aaaccagtca aaggagcgat cgcatgaatt tgactaaaca
     6781 agaaaaaaga atctgtcgtc agataatgtt gcaaattata ggtgatcgtg acatggacga
     6841 attatctcaa caagagagtc gaatggttcg gaagataagt aagctaatta tttatcggga
     6901 tacagaaaga tttctcactg ttttaggtaa cgtctacgag aatccagagt tattggaggt
     6961 agagtaattg ccaaaaatat tagatgcatg ttgcggtagt agattgttct ggtttgataa
     7021 gaacaattct gacgtaacat ttatggattg tcgtcagcag tatgaagaat tatcaacagg
     7081 tcatgtgatc aatgttgatc cagatgtggt cgcagacttt cgcgatatgc cttttgaaga
     7141 tgatgtgttc gatatggttg tatttgaccc gccacactta agacatgtcg gcgaaaactc
     7201 atggcttgcc aaaaaatatg gaaaattaga cgaactttgg ccagaagaca tcaggcaagg
     7261 ttttgcagag tgtatgcgag ttttacgacc ttcaggaaca ttaattttta agtggaacga
     7321 ggaacagata ccactttctg atgtgttaga ggctattgga gaacagccat tgtttggtaa
     7381 taaacgaagc aaaacacatt ggcttgtgtt tatgaaaaaa ttcagctaac ggaggaaaca
     7441 aaggaggaaa agtaatgaag attgtattaa ataaatgttt tggtggcttt gggttgagcc
     7501 atgaagcaaa aatggaaatt tttaaaagaa aaaatattga agtattccca tacatcaata
     7561 actttagcta tgactcagat gatgaataca cgagatacac aggccaaaag ctaggttcga
     7621 tggattttat ctactatttc aaaaaagacc cgaaaatcga caaagttaca ggaatttata
     7681 gcgaaattga ccgactgtac ggaattgctg atgattccag tttttcaagt gattcaaaca
     7741 gaggtgataa ggacctcgtt gctgttgtcg aaaaactagg aagtgaggcc agtggaccat
     7801 atgcaagtct taaagtcgtt gatatacccg atggggctga gtgggaaata agtgattatg
     7861 acggcgttga aactgcacat tatggttttc aaacaggtag tatttagtcc actaatgaca
     7921 actaaagagg aggcagaagg atgagtgaag atgaatactt tgaacagtag tctattaacg
     7981 acagagattt ttaactggaa aggggtgtag tcatggacgc atcggaagta gtaaataaaa
     8041 ttaatagtct caaagaggtt ttcggagacg tcgaagtagt gataaacgca caaggctatg
     8101 acaacggatt ttttaagcag attaccgaca ttagcattca gcaaggaatg gaagacgaag
     8161 atggaaactt tatcgatgaa gtagccattt tagtgacttg cgagtaagta attgacagct
     8221 aaagaggagg cagaaggatg aaaaaattgc accaactcat atctgagaaa gaaagcgaac
     8281 tccaaaattt agaagatagt ttgggattgg ggtttccaat tgttgagcaa gctaaaatga
     8341 ctcaaatcag tcatctacga cttgaattgg aagatttaag acaaatagaa aaatcaattc
     8401 aactcaacga caaccagaaa atcgtgttgg agtggttgaa aagcgagaca attttaacta
     8461 gagaagcacc aatattatct gttaatgctt tttctgataa aaatttacta ggaaaattac
     8521 ctgataaagt acgcaaagct tataaactat tggcttgtaa acaagaatat gaagtcctat
     8581 cagcattcgc tcaatgggga ttagaacagg aggaagcgga atgaacaaca gacatcgcag
     8641 aataacaaaa ctaagaaaac aggaactgaa tgtactaaag acaaagtttg aaaaagaata
     8701 tggaatttca gcagaagaaa catataaagt ggcaagtcag tttgttgctg atgcgagtaa
     8761 tgctattcgt aagtttggga tttcgatatt aaatgatgat cgtaaatggg aggaaatgag
     8821 atgaaaataa aagacggatt ttacgctagt agtcatggta tcggcggttt aatgctagac
     8881 atgccgataa agaaccctaa aacacgtcag aaatcaaaat tcaaaatcgg tgacatggtc
     8941 cgatgtaaag cagaagagtt catctatccg tttcgtggat atgtagagca tgtctataat
     9001 cattcagcaa tcattcacat tgaaaacacg atggaatgcg ataagtggtt agcgaaaagc
     9061 aaagataatt tagcagtggc tcgtttggtg gatatggaac taatcaatga caaataaaaa
     9121 agccggatcg ctccgactga ttcaataaat ccaacacatt tattatatca cataaaggag
     9181 cggtttgact tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg
     9241 agagatgtgc tgaagaactt tcggcgtttg gagcggatgg caggtcgctc tttgatagat
     9301 attaagtcgc cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa
     9361 gacgcgatca ttcagatgat ggatatagaa gcggagagag acgcaatact agcggctttg
     9421 atggctctta gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag
     9481 cactctaatt atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag
     9541 ttgaaatcca tcgcattaat cgaatttgca gaagcataca aaaaaggcgt gttagttcag
     9601 tatcgttgat tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt
     9661 gatattatgg tagtgtcgaa agattagtga taggtctgag acaaaataaa atgtaaggga
     9721 ggaaatctcc ctcatcgttt taacttaagc ttcgatagac agcaacggaa atattaagaa
     9781 taaggatgtg aatttcaact ccttctaaat tgttcttatt atctatcatc cgttgttgtc
     9841 tattaattta tgcatcggag gaaaaacgaa tggataaaga aatcaaagta actgtcaaat
     9901 tagatttgac tgaactaaaa gaactgctca acaaggctag tgaccaagtc gaacagttac
     9961 aagaaacttt agatgaaatt gctaatttta aaatccaagt ttcttagcag tatatttttt
    10021 agcagctgca ctcataaaat cataccaagt atcagaaaaa tataacttaa ctaaataatt
    10081 aaaaaaggtg ggtaaataga aatgacggcg ttaataattt caatttttgc gctctgtctt
    10141 aatgtctata tgattggatt taaaaatgga caaaataaaa aatagtagca gccgagaata
    10201 attttatagt gtcactgtgg cggaaataga agacgcaaag gtggaggtag gaagcgtatg
    10261 gcgcactatc gggaccttgt acaaaactag aaaggactag tacgtgtgtg gtgcgagccc
    10321 tagggaggaa caggataacc gcctgtgtgt aggttgctat tacatattag atcactcttt
    10381 gagtggtctt tttatttaca taaaggaggc tacataatga gaaactactg gtatatatcg
    10441 ctaactaatg aatatcctcg aaccattgat gattgttcag tgcgtgttgt gcgttctgtc
    10501 caaatcaaaa agaagtactc cattgttgaa atgaccagag aagctacgcc aaaagagatt
    10561 gataaatgca aacttcttta ttgcggtcat ggtttctatg atgaaccaaa cattcaaaat
    10621 aatattaaca agaatttgag ggattagaat gcaagaaatg gcttaaaaat ctccaatcag
    10681 gacaaatcaa gttagacaaa gtttccgatt taaagatatt gattgaagct gatttaatgc
    10741 taaaagatat tgaaaattag aaaacaaaac tcaacctaag aagattgcga ggtggtgtgt
    10801 attgaatggc aagacaacgt gatccaagac gtgatgaagc caaaagaatt tggttagaat
    10861 ccaacggaga aaagcagtta aaggaaattg catctgaatt aaatgtttca gattctcagg
    10921 ttagaaaatg gaaatcgcaa gacaaatgga gcgctgaatt gaaaagtaac gttaccaatg
    10981 gcaaaagtaa cgttactaat caaggtggcg ctcctattgg taatcaaaat gctaaaggta
    11041 acaaaggaaa cagcagagca tcaccgccaa agagaaataa gaatgctctt aaaactggtg
    11101 agtatgaaac aatatttttt gacacgttaa ccgataatga gaaggacatc tattctagtt
    11161 tgaatgatga tccttctttt gctttgtccg aagaaatacg acttcttaaa ataagacaac
    11221 ttcgaatgat gaaaagaatc caacaagctg aagctggact aaatgatgaa gaagtcgaac
    11281 gattgcagca gctaagaaag attaaaactc cgattgaaaa aaatggtaaa aagctagaaa
    11341 tcaagcgtga ggttatgcaa gatgtgcaga ttagcagaaa aaaacatcgc aaaattgatg
    11401 atattctttc aattgaagat tcattgactc ggattagcaa ccagttagct aaagccatca
    11461 agcaaatgaa tgaactttat atgaatgaat acagaactga tttaattaaa gctcagactg
    11521 ataagatcca agctgagaca aatgaaattg gcggaaataa ttcaggtgaa gaaatagaag
    11581 aatggaaaca ggcagtttta aatgccgcaa acaaacgggc ggtgaaagaa aatgaataat
    11641 gaatttattc cttttgctga tattggttct gccattgatt attactatga taaaccagta
    11701 gctttttgcc aagatatttt gcatttgaat cctgatgaat ggcaagaaaa tgttttaaat
    11761 gatttagctg aattttcaaa ggtttctgtt cgttctggtc aaggagttgg aaaaacagca
    11821 ttagaagcag gagcaatact ttggttctta acgtgtcgac cctacgctaa agtaatagca
    11881 acagctccga caatgaagca actttacgat gtactttggg cagaggtagc taaatggtta
    11941 aatgatagct tgatcaaaaa cttactgaag tggacaaaga ccaaaattta tatggttggt
    12001 gattcagagc gttggtttgc tacggctaga acagcgacta aaccagaaaa tatgcaaggt
    12061 tttcacgagg accatatgtt gattgtggta gatgaagctt ctggtgtgtc tgatccaatt
    12121 atggaagcta ttcttggtac gctatcaggt tttgataata agctgttgat gtgtggaaac
    12181 cctaataata ttgaaggtgt cttttacgat tcccacaatt cagaccgtga taaatacaga
    12241 gttcataaag tatcaagcta tgatagtaaa cgtacaaaca aagacaatat agaaatgatt
    12301 cttaaaaaat atggaaaaga aagtgatgtt gctcgtgtcc gtatttttgg agaatttccc
    12361 aaaggtgcgt tggattcatt tatcagtctt gaaacggttg aattggctac agaaaaacaa
    12421 attagtgatt ctttagtcaa taaaacaacg gttgctcata ttggtgttga cgtagctcga
    12481 tatggtgatg attctacgat tctctttcct agaattgcta ccagggcatt ggagtatgag
    12541 aagtattcaa aacgtagcac tatggaaaca acaggatatg tcatcaacat ggccaagaat
    12601 ctaatgagtc aatatccgag tattgataaa gtgatgatta aagtcgatga cactggtgtc
    12661 ggaggtggtg taaccgaccg cctagaagaa cttatagaag acaagcatta tccttttgag
    12721 gtgtttggag tgaataacgg ttcaacatca gaagacgatt tttacgataa tttaggtact
    12781 caactatggg gaaacatcaa ggaaatgtta gaagaaaata tgacagcaaa tcttaacgga
    12841 gaacagcctg ttattgaatt gccttctgat agttcgttaa tcaaagaatt aagtactcgc
    12901 aaattcaaaa tgacaagtag aagtcgtata cgtttagaaa gtaaagatga tatgaaaaag
    12961 cgaaatattg gtagtcccga tattgctgac gcactggctt tagcgtttta tgagccacca
    13021 agtcactatc aatttattca attttaggag gtgagttttt gattagtaca gtattatctt
    13081 tagagagata taagaagcta cgtgttaaat atgcaacaca aattgaagat ggaatgtttg
    13141 atccgaatgg ttttatagaa tatatgaaac cattttttgc tgatcgcgaa agaaaatatc
    13201 tagcttatac tagcgaaaag aatgaaatag acaatagacc aaagcctaac actgatattg
    13261 taaaagttaa taataaactt catgctggta tgtattcaat tgtcgtagac caagcagtca
    13321 atcattttac tggtatacct atcaaatggg attacgatgt atcggaacaa aaaagaacac
    13381 tcattcaaag attaaaagat aaattcttaa aaaatgatat agaacttcca acagttccag
    13441 aagcttttaa caaattaaca agcaatcttg attccatgag attcgcaatg cttgattctg
    13501 aaactgcaac ttttcaagga gcttgtggag tagctttccg attgttagag cctgtggaag
    13561 aagatgatgg ttggaagtta agagcaagta atattgaacc ttggagagca gaaagatatg
    13621 gaaatgccgg aatctatatc aaagaaaagt atgactctta ccagaaaaaa ttttttcaag
    13681 aaatgaaagt tatgacaaga aataaaattc tgacatatgc ttgctatggt gatttgaatt
    13741 ttgttacaag cggaacgttc aaaaaaattg atgagaccga taaccctttg gggacgatta
    13801 ccttggcaga atttaaaaat aatacgaatc gttattgtga ttttgaagta gctgaggaaa
    13861 ttggagatgc aattgatcgt gctttgtctg atcaacaaaa cgaaatcgaa caatttaagc
    13921 ttgcgtacat gcttgttact ggcacaacaa tgagcaaagg cactgctaaa gaaatgatga
    13981 atcagctagg gattatcaac ttaaaagacc ctactgcaaa agctgaatat gtcacaaaaa
    14041 aattagcaaa agatttcaat gaatatcata tggatctatt gaaaaaacag ttttacacca
    14101 tttgtaaagc aatcgatttt aacgatgagg tgtttaaatc aaacagttct ggagaagctc
    14161 gcaagtggca aatcattagc ctagaagcga aaacaaatac taaagagcaa tattttagag
    14221 aaggattgaa agaatgcgca gaaacaattg ctgcttttct taagtttcat gacaaagtag
    14281 aaattgagcc tgaaaaaatt atttttactt tctctagatc tttgccaact gatttaagtt
    14341 atttagctga agctttacct aaacttgcac catacgtatc caaacgaact attcagagcc
    14401 aaattccatt tgtaacagat gttgattatg aaaatgaaat gatggaattg gaaagtggaa
    14461 gtgcttatcc agatagtgaa tacaattttg gcggaggtgg caatagtgac gatagaaacg
    14521 aagtattgga ccaaacgtcg agaactggag gatcaagcaa ggctcaaaca agaaaatcag
    14581 acacttaaaa aattaactag tgtatttcct gaagcactga aagagataca agcaaaacta
    14641 ttatcacaag ctgacttaca caatatcact tatccagaaa tgatggagtt ttacagtaca
    14701 agtaatcaga aaaaatatcg tgaatatgtg gaaaaaaatt ataagtcatt aaaaatgtat
    14761 gatgcaaaat acaaagagtt tatcgatgaa ttttttccac catttgacta tgcaaaagtc
    14821 aatcgcttat tacaaatacg atcagatgta tttaaaattc ttgcagaata tgcgatggat
    14881 gcagatgtga atcaatattt ttctgatcgc ttagaggaaa ttcttcaaag aacatattct
    14941 tctaatgcta atgtttttgt tcaactttta aacgttgata taccaaatta tttaccagaa
    15001 aatgaaatta aacattattt gaactatcca tggtcaggaa agacattttc aagaagactt
    15061 tggggaaata tttcatcgct tgaacagaaa ctctctaatg ctattgtaaa aagtgttgct
    15121 agtggagaag gcgttataca cgcattaaac accatgagat tagactcaga aatttgtgac
    15181 atgtttaagt tagaagaatc aaagtataac aaagcgatag aaaatctcgt tcgaacggag
    15241 tatgcaaaat ttgcacaaga tggtattgaa aaatcatatt tagaaacagg tattgaggaa
    15301 tacaacgtat tgactgcaaa agatgagaga gtttgccgga tttgtggagg aaaggcaagt
    15361 aagaatccct ataaactgaa agatgctgtc ataggtgaaa atcgagcacc tttccatagt
    15421 cgttgtagat gtacggatgt tcctaattta ccaaaattag gaaaggatat tgatgaagaa
    15481 tatgaccgtt tatttggcga tttattagat gagtttgcac atgattcttt tggaattaat
    15541 ttgaaacgga ggaagtagaa tgaaagattt ttttgaagca gtactaacaa ttaatgtaaa
    15601 tgctgatatt gcagaagcct acaaaacagc tattgagtct gagaaccatc ctaatggctt
    15661 gagagaccat tggaatggca attatgccta cgtggttatt ggcgatcaaa ctgttaatta
    15721 tcaagataat actccagttg ataagaatac cgttaattta acgattcaat tattatctca
    15781 ttcattacca aatttaaaag aaacagttga ttggtatgaa aagatgggat gtattgttgt
    15841 tagaactgac tacaaagaag gaaagtctag taattaggct tttttatttt gtccgaaatg
    15901 acgttaaact agcgcaatac tgggcttggt tgaatggtgg ggcgcaacta ttaaaactca
    15961 aagcaatgcg gggcgtgaaa acgaatcgtg gggcgaaagg agaatgagca tgaaacacaa
    16021 atcattaatg ccaatgaatt tgcaatactt tgccgaaggt gacgatcaaa agttttcttt
    16081 tgatgacttc aaatcttttg tggaatccaa tgaagaagct caaaaattta ttcagtcaca
    16141 atctcaaagt gttgccgaca aacaattgga agcttggaaa caaaataact tagataaaat
    16201 taaacaggat accatcaagg aatatgagga atctaagaaa aacaagtcac ctgagcaaat
    16261 tcaactggaa aaactacaag ctgaatttga agcagaaaaa gcgttgcgtg tgacaagtga
    16321 taataaagca tttgttgcag aacaaattgc tggattagaa ctagatggag agttaaaaga
    16381 gtctatttct caatttatgc taaataatct tgttagttcg gatacagatt tcactaagaa
    16441 tgctgttgaa ggttttacaa gtgttttgaa tgcaattaat gagaaacatg cagatgcatt
    16501 aaaagaacta caaatgaagt ctgcatttgg tggaactcaa caatcgaata accaggttca
    16561 gcagaacaat gaaacattaa caaatccaga agaacaatta ggacaaattc ttcaacaatt
    16621 taactaggag agtgaaaaat tatgaaaaaa acatctttaa ataatttaga gtatttggat
    16681 atttcaccag cgattaatgc tatgcaagta ccaaatacac cttttttaag ctatttactt
    16741 ggtgctggaa aaacagagcc agcaaactcg acagaaatta aatggcgtga atatgatatc
    16801 aacaacgatg attcttctga aaaacttgag ggcggagaat atccagatgc tgaatcaggt
    16861 cgaacttggt ttaacaacta tactgaaatt tttagaaaat caacctctgt atctggtaca
    16921 ttagatgcta ttaatgtgaa tggagtcgga aatgaattaa ctaatcaagt agcactacgt
    16981 ggtatggaaa tgaaaattga tttgaaccga aaattgatta ctggtgtaaa agctgatgaa
    17041 aatggttcta aaggtcgtcg aatgaatggg attttgaact tgatcaattc agcaaataag
    17101 gcagaaacag ccactgcggg tgcagtaaca agaaaagata tcgatacttt atttaaattg
    17161 atgtatgaaa aaggttatat gggagaaaaa ctatgcttga tttctccaga tatgcaggag
    17221 ttaatgactg atgagttaga tggaaaatca acaaaaattg ttcagttcgg ggaaagagta
    17281 acttttggat tgcaaattgg aaatatcgtg tctaattacg gtacaggtat tgctctacta
    17341 gaaccatcat tgccaaaagg aacaattgcc gcaatcgata ctaattatgt gaaactacgt
    17401 ccattacgtg aatggagagc agaagaactt gcaaaaacaa cggattctag acgtatcggt
    17461 cttgtaggtg aatactctct tgaatacaat gcttcaaact ctggggcaat tttaaattta
    17521 aagtctgaat aaaagggagt tagtactccc ttttttgata ggaggaatta cagtggtaaa
    17581 aaaagatgaa actaaaaaag atgaagtcgt gaaatataga gtaggtaaaa ctaaaaattt
    17641 tgttgggttt gttcatccta aaactcgtag atttatcaca gcagattcaa ataacgaatt
    17701 tatcatttct atagatgata aagaagcaat tgcaattttg gaagatgcaa ttgatgttaa
    17761 tgaaatttag gaagtgatct gatggatgaa tcgctaaaaa cggaaatcat tgagtctaca
    17821 aaagaagatt ttccagattt gagtgaagaa cgcataacta atttattaga aataattttg
    17881 ctagaaattg aatcatacaa cacttgtaaa aatgatattt catgggaaaa gttaaaaagc
    17941 gtgattagtg aagtgttgta taaaataata aaaaatgaat cagaaaaaac agtatcttca
    18001 gttagacgtg gcgatacaac gattagttat gcttcaacaa caaatgatgt tagtgagtta
    18061 cttctaggct atggtgattt gatacgaaga gttattggtt gtggaggatt ggagtttttt
    18121 taatgaatga agcagatatt ttagaaacta cttatgaaga tagttgtatt attgaaagac
    18181 tgacggacat tgaagattct aatacaaata ttactattca agattacaaa aaagtatatg
    18241 ataatcctat ttcttgcgct ctttcacaag gtcaaatcga tggactagca gtcatagaag
    18301 atggagagat ggtaaatgtt tcaactgaca catataaatt atttgttcat cctaagatta
    18361 aactaaaaaa aggagatcga ataacaataa ctcaaaaggc cagtggctta attttttctc
    18421 tatttgctac taagcctttt tactatccta gtcattgtga agtgaatttg ataggaagtg
    18481 agaaaaatgg gtaatctcaa atatgaatca aatgcagaaa agatcattga gaattttaaa
    18541 aatatgactg taattgctca aaaagaagga atatccttcg ttaacgattc aatgaataaa
    18601 gtcgttagtt tagctaaacc attaactcct gtaaaatcag gtaatttaag gcgaggatat
    18661 cgtgtagtaa aagctagaaa actatcaagc ggtcgaattg ttggagcagt tataaacaat
    18721 gaacattatt ttaaatatgt agaagaaggt cataggacta aaaatggtgg ctttgtcaaa
    18781 gggaaattca tgttaactcg tgcaacgaat cttgcaaata tgtcttatat tcctcgaaga
    18841 tttaaacaaa tggcaataaa aatcgttaag aaaggaaagt aacatgaaag atgaaatcat
    18901 tgctgcaatc agtagcaaat taaaagaaat ctatcctgat gggacaatat atcttgattc
    18961 agttatgcag tcaactaaag atttttattt cgttttatca gtaatggaat ctggaactga
    19021 aaatgtagga attgatattc aaaatgtttc tttcttaatt gatattgcat tgattgataa
    19081 taaacctaat agaaatttaa ttaatgaatt agtctcacgt tgtgggactt tttttaatac
    19141 gattacaatc gatgagcaaa tattatttcc aaaagcctat ttacctgatg aagcagatgg
    19201 tgttcaacat attcgtttta cattagaatt tccacaatat attgaatgga gtgaaagata
    19261 aatgggagaa aaaagaagta aaaccggaat tatttctgtc gaaaaaccta cttggtttcc
    19321 tttggaagat gagacaggga attttccggt atatggtact gcgttgccaa tgggaacagc
    19381 agtaagtatc aaaccaacag ctaattatga aacaacacaa gattatgggg attcagtggt
    19441 tcaagatcaa tttacagctt ttggtggtgc agaagttgaa ctggaagcaa atggatatac
    19501 tcataaagtt ttatctacaa ttactggagg gaaaattgtt aaaggtggtg ccttgcgttc
    19561 tggagaagat attgcgcaag atggtgcgtt tgcatataga cgtaaaaaat cgaatgggaa
    19621 ataccgttat acggtctttt ataaaggaca gtttgctcta gattcagatg aaacatcaac
    19681 gattgaagga agtaaagtaa gttttactca tccaacttgg aagggttcat ttgtagatgt
    19741 accaggactt ggatatatgt attcagttga tgaagatgat gaaggcgtgg ataaagcaat
    19801 gatcggaaat tggtttacaa aagtagcaat tccaattgaa gaagcagaac tttcaggagg
    19861 tacagaataa tgtcaaaata tcaaacaacg attaaattaa cgaaaaaaga tgaagaaggt
    19921 aaatatgagc aagtacaatt taaatctgct gaatttctac caggcccagt cgtagaagaa
    19981 gcagcagaag taatggaggg aatgcaaaat gctgtagatg gaaaatcagt atcagaagca
    20041 ttaagtcgag cttattcatt tattgcagat attttgtttg aaggacagtt tactggtgaa
    20101 gattatcgtc aagggatcga tgctcgagaa attgcttcgt tgacaggtaa gttattaaaa
    20161 tctgttactg caggctttga tgaaacttat acagaaacga aaaaaaagta agcgaagctc
    20221 tcaaatatcc tttttttaaa cattcgatta cttatcgaga attagattta aagacgcaat
    20281 tactcgaagc aggttggacg ttaccagaaa ttgaacgtac tgactttgac gagttaatgc
    20341 gtctttttgc ttttcgagat gcagtaaaag agcatgagga tgttgagtac tacgataact
    20401 tcacccaatt ttaggaggtg atactttgaa taacgatgac ctaattctga agatgatact
    20461 agatgaatct ggttttactg ccggtatgaa taatgctgtc aaaaagttga actcttttga
    20521 tgaaacaatc gaaagaaaaa gtagaaatag tggcagttca ttaggtagta tttggaaaat
    20581 atttgctgga agttttctag ctagcggagt aactagaatc gtaggagctg gctttgatct
    20641 aatcaagggt tccataagtg gagcggttga tcgagtagat acgatgaata acgctctacg
    20701 aaatttccaa aatatgggat ttagtaattc agaaattatg aagaatatcg gaaagaatgg
    20761 gcttttatct caaggtattc aaggacttcc tactgctttg aatgatgcga taagtcatgt
    20821 tcaacttctt gcttcttcta caggtgattt aactcgttca actcaaatat ttaaagcttt
    20881 aaatgatgga attcttggtt tcggtggttc aactgatcaa gttaatgaag ctgttattca
    20941 attatctcaa agtttctcaa acggaaaagt agacgcacaa acttggaact caatgatcaa
    21001 cgctcaactt ggacctactc tttctgctat tgctaaaaag atgggaatta caatgggaga
    21061 tctgaaagaa ggtttatctc aaggtaagat ttctgttgaa gaattccaaa atcaattaat
    21121 agaaatggat accaaaggtg gcggaggact taaatcatta agtcaaatcg ctaaagattc
    21181 tactaaggga attaaaacct ctatacaaaa tgcaaaaaca gctgttacac gtggtgttgg
    21241 tgaggtaata gaaggattaa ataaagcatt agtagattca gatttaggtg gatttaaggg
    21301 aattattgat aaagtgggca gttcaatgga atcgttctta aaagtaattg ctgcaaatat
    21361 tcctacagca gtatcatttt taagtaattt atttaacgaa attcaaaaat ttggttctgc
    21421 tttgaaattc atgatgccct ttcttgttcc tgcagctacc gcttttggag cgttcatgtt
    21481 tcaacttaaa ggtataccag caataataaa aagcttcaat aattttaaga atgccataat
    21541 tggcgttgga aattcattaa agattatggg ggcaatagct gctgctaatt catttgtttt
    21601 aattattggt gcagtcgttg gtgcaatagc tgttttcggt tactttatgg caaccaatga
    21661 agagcttaga aacaaggtta tatctatttg gaatgatgtg aaagattccg taattggtgt
    21721 attaaaaaat ataaaggact ggggaattga tacttggaat tctgctaaat aaatggcatc
    21781 aaatgcagtt gagggtgtca aagatgcttg gtcaggaaca aaggaatggt tttctaacac
    21841 ctggaaaggt attaaggatg gggccacagg tttatttgat aaaacagtag agacttcaag
    21901 aaatgcagtc gatagtgtaa aaaatgcatg gtctaatatg aagaaatggt tttctgatac
    21961 ttggcaaagt ataaaagatt cagccagaga aaaatggaat ggaatcaaag gttctattat
    22021 gtcagttgct ggtccattga ttacaggtat aaagaatgca tttttacatg ttacttttta
    22081 tctagagacc ttatggaata atttagttga gattggtaaa aacgtctttg agattttaaa
    22141 aaatgttata cttgcgccgg ttttgtttat tacctccatg atttctggtg gatgggaaga
    22201 aacaaaaaat aatatgattg ctgtttggaa taatattaaa gaaagtgcca taaatatttg
    22261 ggaatctata aagaatatat ttgtaagtta ttttacaaat atttattttg ctgcactcaa
    22321 tatttggaca gggttcaagc ttacattgat aaatatttgg aatgaagtgg taaatcaagc
    22381 taaatcgatt tggttaaaca taaagtattt ttttattaat ctttggattg atataaaata
    22441 ttttgcaatt cagaaatgga ttgaattgaa gttttctatt attcaaactt ggatcga
//
LOCUS       DAZAID010000047        22175 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000047 DAZAID010000000
VERSION     DAZAID010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.047"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            240..2075
                     /gene="tdc"
                     /locus_tag="ACG9BE_001629"
     CDS             240..2075
                     /gene="tdc"
                     /locus_tag="ACG9BE_001629"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFN3032373.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2380..3858
                     /locus_tag="ACG9BE_001630"
     CDS             2380..3858
                     /locus_tag="ACG9BE_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3032374.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3824..5155
                     /locus_tag="ACG9BE_001631"
     CDS             3824..5155
                     /locus_tag="ACG9BE_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3032375.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5269..7821
                     /locus_tag="ACG9BE_001632"
     CDS             5269..7821
                     /locus_tag="ACG9BE_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFN3032376.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7828..9267)
                     /locus_tag="ACG9BE_001633"
     CDS             complement(7828..9267)
                     /locus_tag="ACG9BE_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3032377.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9242..9424
                     /locus_tag="ACG9BE_001634"
     CDS             9242..9424
                     /locus_tag="ACG9BE_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032378.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9613..9975
                     /locus_tag="ACG9BE_001635"
     CDS             9613..9975
                     /locus_tag="ACG9BE_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFN3032379.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10162..11019)
                     /locus_tag="ACG9BE_001636"
     CDS             complement(10162..11019)
                     /locus_tag="ACG9BE_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN3032380.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11139..12050
                     /locus_tag="ACG9BE_001637"
     CDS             11139..12050
                     /locus_tag="ACG9BE_001637"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFN3032381.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12057..13708
                     /gene="mdcA"
                     /locus_tag="ACG9BE_001638"
                     /pseudo
     CDS             12057..13708
                     /gene="mdcA"
                     /locus_tag="ACG9BE_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13810..14109
                     /locus_tag="ACG9BE_001639"
     CDS             13810..14109
                     /locus_tag="ACG9BE_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFN3032382.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14099..15775
                     /gene="mdcD"
                     /locus_tag="ACG9BE_001640"
     CDS             14099..15775
                     /gene="mdcD"
                     /locus_tag="ACG9BE_001640"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFN3032383.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15779..16372
                     /locus_tag="ACG9BE_001641"
     CDS             15779..16372
                     /locus_tag="ACG9BE_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFN3032384.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16396..17472
                     /locus_tag="ACG9BE_001642"
     CDS             16396..17472
                     /locus_tag="ACG9BE_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN3032385.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17497..18366
                     /gene="mdcB"
                     /locus_tag="ACG9BE_001643"
     CDS             17497..18366
                     /gene="mdcB"
                     /locus_tag="ACG9BE_001643"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFN3032386.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18477..19241
                     /locus_tag="ACG9BE_001644"
     CDS             18477..19241
                     /locus_tag="ACG9BE_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFN3032387.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19730..21823
                     /locus_tag="ACG9BE_001645"
     CDS             19730..21823
                     /locus_tag="ACG9BE_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN3032388.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21848..22150
                     /locus_tag="ACG9BE_001646"
     CDS             21848..22150
                     /locus_tag="ACG9BE_001646"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3032389.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7179 a         3788 c         4725 g         6483 t
ORIGIN      
        1 tgtttgttcg ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc
       61 gcttttttgc gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc
      121 tacgtggatt tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg
      181 ttacagataa acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa
      241 tgaaagatat ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt
      301 ataaaatgtt gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc
      361 cttcagatat gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc
      421 gcgaccatat gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc
      481 catggcattc agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt
      541 tattagctta caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta
      601 tggcaacttc gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga
      661 cagacggatg gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt
      721 acgcccgttg catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga
      781 aaaaaatgtc tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg
      841 aaagctttac ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa
      901 atatccaaag attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg
      961 cgttagatat ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt
     1021 atcgaatgga tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc
     1081 caattctagg tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag
     1141 ataaaatcgt ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg
     1201 ttgatgccgc atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg
     1261 tgccgtatgc ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa
     1321 cgattgacaa agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga
     1381 tagatcctca taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata
     1441 aaaatatgag aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg
     1501 ctccagatat gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg
     1561 cagtctggac agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg
     1621 gggcttcaat cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg
     1681 ttaaaggaaa aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt
     1741 gggtattcaa agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa
     1801 tgttcgatcg gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc
     1861 atactacatt tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg
     1921 gtctaacgaa agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca
     1981 tgactcctta tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag
     2041 caatggaaaa gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa
     2101 caagagacat atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct
     2161 gtttttcata tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc
     2221 gttcgtttca tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac
     2281 tgggttttgt actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga
     2341 aaacaaaaac atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca
     2401 aagcaaatca cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga
     2461 aatattccag atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta
     2521 ttattgtacg cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa
     2581 aaaggtggtc cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc
     2641 tcatggctgt tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct
     2701 ccgttgtttg gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta
     2761 gttgtcattt tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca
     2821 aaaatcggcg gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc
     2881 ttattagggt ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt
     2941 ttttcatggg acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg
     3001 ccgatcatgt ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa
     3061 aatccagtta aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc
     3121 aatattttga atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat
     3181 aatatcgcgc aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg
     3241 aatctgttta gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca
     3301 ggaccagcaa agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat
     3361 ttttggaaga cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt
     3421 atctcaatct ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg
     3481 ctcgtaaatt caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt
     3541 ttgagattgc gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag
     3601 aaaaagagca atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt
     3661 tctgtcggac tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc
     3721 agtgtcattt tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa
     3781 caggaagtcg ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat
     3841 gaaacggact ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac
     3901 aggattattt ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt
     3961 ttatttgata tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt
     4021 agcaaatagt acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg
     4081 ggcatttttt gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt
     4141 aacagcaata ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg
     4201 aggtagcgca ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga
     4261 attggaatct tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg
     4321 tataggattg agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg
     4381 acagatcgat gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt
     4441 tttattcagt tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc
     4501 gcaaaagaat ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat
     4561 aggaacgatt ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag
     4621 tcctttagtg actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct
     4681 aatgattttg gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat
     4741 tttatatcaa acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa
     4801 aggcgtacct ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct
     4861 acattttttt gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag
     4921 ttgttttcta tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa
     4981 gaaacggaac actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat
     5041 tgctttcagc ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg
     5101 gtttgtattc ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca
     5161 gatacatttt gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa
     5221 aacttccctg cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac
     5281 agattgacgg tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa
     5341 gaagaacgac agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag
     5401 acaaaacgat ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc
     5461 attgcctctt ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc
     5521 gtagtgatcg tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg
     5581 aatggtttga agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa
     5641 aaaatccctg cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc
     5701 gttccagcag atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg
     5761 acgggagaat cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc
     5821 ggcgaccaga aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt
     5881 gtcgttgtag aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg
     5941 gtcgaacaac aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt
     6001 caaggcattc tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg
     6061 gattggcata tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga
     6121 ttgccagctg ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag
     6181 gcgatcatca aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca
     6241 gataagacag gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa
     6301 gaagcctgcg tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag
     6361 aaaacagaaa atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca
     6421 gccaatctac ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat
     6481 atggcaacga tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa
     6541 gtattatttg ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct
     6601 gcagaatttg cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg
     6661 aaacaggaac tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg
     6721 atcgatccgc caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc
     6781 tcagtgaaaa tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt
     6841 ggcttaaaac atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa
     6901 gagttgattc agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa
     6961 ctgcgaattg ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc
     7021 gtaaatgatg cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt
     7081 agtgaagtaa gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt
     7141 gcaaaagccg taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc
     7201 ttcttgccaa ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc
     7261 ccgctacctt tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta
     7321 tcttatgcac tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat
     7381 ccaaagcaag gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg
     7441 atcatggttc ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc
     7501 attcttctgc aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg
     7561 tccaagccgt cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt
     7621 atccttgctt tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt
     7681 acagtaagtt taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc
     7741 ttagttgtag aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca
     7801 gctagagaat acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa
     7861 acgattgagc tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga
     7921 atctagaggg aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc
     7981 ggttagttcg ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg
     8041 caagaacgcc ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg
     8101 tttgatagaa aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg
     8161 ttcattgatt tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa
     8221 gtgtgcatat tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc
     8281 aatcaatatc ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga
     8341 tgtttttttt agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag
     8401 gacctctaaa aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt
     8461 gtaattccaa ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata
     8521 aataccttcc gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct
     8581 gttggtatgg atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa
     8641 gaaccaacat gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt
     8701 tcttaaaaat tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt
     8761 atgagtgaaa ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag
     8821 aatacaagct gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt
     8881 gattttagtc acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc
     8941 agggacagca actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg
     9001 tttgaaaaaa atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat
     9061 tcctgcttga tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag
     9121 gtcagaaaaa atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat
     9181 ctttgtatga ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt
     9241 aatgattttt ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga
     9301 gaaaataaac ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc
     9361 atttactgat gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa
     9421 ataggtgggc aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc
     9481 ggattgaaca tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta
     9541 cttgtattca tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag
     9601 agatggaagc agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat
     9661 ttgtctccag aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa
     9721 agcaaaaaat ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca
     9781 agtgaccata ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt
     9841 ttgcgaggaa aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct
     9901 attgatctac gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt
     9961 aaatcaacgc aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct
    10021 tcaagaaata agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt
    10081 attttctaag aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat
    10141 tttgtcccag ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat
    10201 cattttgatc aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa
    10261 gggcaaatct ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac
    10321 ttttgggaaa aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc
    10381 atctgtcgtc gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt
    10441 atgacggaag gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa
    10501 ttgcagatgt tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc
    10561 tttcatgata ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg
    10621 atcggtaata tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt
    10681 ttccataaaa attcggacca agttcggaat agaaacagag gctacctcta aatacaatat
    10741 gacggaagta acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt
    10801 gtctaatgtc ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt
    10861 tctgccgcta tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg
    10921 gctcaaggca ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc
    10981 gaatactttt ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac
    11041 ctttttgtta tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga
    11101 tagcagtgga aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc
    11161 ctggacaagg cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac
    11221 gtttagaaaa tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg
    11281 tttttatcca gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa
    11341 acggtattca gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg
    11401 cttgtcgatc tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa
    11461 tgaaagaagc ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg
    11521 aagctgagga aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg
    11581 aaaattgtcc gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg
    11641 cagaagccaa acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc
    11701 actgtctctt gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc
    11761 tatctgatgc tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag
    11821 aagagattcg taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga
    11881 tggacttggc aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt
    11941 tgacaaagct gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag
    12001 accaacatgg tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg
    12061 aaaaaccaaa ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa
    12121 tcagcaactt gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa
    12181 aactgattgc tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt
    12241 ttctttcaga aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca
    12301 tgtctagtat ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga
    12361 tcgatttctc ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca
    12421 caatgaaatt aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt
    12481 tgattccaaa tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca
    12541 caggtttcaa tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta
    12601 tcgtgatcgt tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg
    12661 actgggtaga cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac
    12721 gagatcctca aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa
    12781 tctacgcaga acacggcgta caatctttga atcacggtat tggattcaat acagcagcta
    12841 tcgaattgtt actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga
    12901 actgggtatt gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa
    12961 gcatccatag ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg
    13021 tcttttttgt cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag
    13081 gacaatatgc catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact
    13141 cttctaccgt aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata
    13201 atcctggcgg tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc
    13261 cattagggaa aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa
    13321 aaccagtctt tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca
    13381 atgcaatgca ccagtgatga tttatagcga agatactaca catatcgtga cagaagaagg
    13441 aattgcttat ttgtacaaag caaaaacaaa agaagaacga caagcagcaa ttgcagcaat
    13501 tggcggtgtt acacctcttg gaatgactag cacgaaagaa tcactagata gcttgcgtgc
    13561 agccggcatc gtgaaattac cagaagatct aggcatcaaa cgtagtgaag cgaaacgttc
    13621 attattagct gcacaaacca tcgatgattt gatcgaatgg tcagatggcc tgtatgaacc
    13681 accaatgaaa ttcagaactt ggtcataaaa ttggcgagaa aaaccttttg tcgccattta
    13741 cccagatgga agaagaaaga cgaataagca gaacgtgtat gctctgtttt acaaaaaggg
    13801 gagagatcca tggaacaatt agcttataaa tatccaacga aacagccgat caataagaaa
    13861 atccatgtag gagtggtcgg ttcaggagat ttggaaatct tgatgtttcc aacagaaaaa
    13921 acagaatcta ctgtaaaaat ttgtacagga agcgatggct tcggcgaagt atggaacaat
    13981 gtattaacga gattttttga tcgttatcca atttcagcag atatcgtgat caatgacttc
    14041 ggtgctacac caggagttgt gtacctacga ttgacgcaag caatggagga actttcagat
    14101 gaaaaatagt tttgtagaat taaacgccag agaacgagtg aacgctctac ttgacaatgg
    14161 acgtgaattg attgatccat tcgatcaaat catcgcgcca agtttaccag cacaaggaat
    14221 cgtccctgaa agtgatgacg ggatcgttgt cagccgtggc acgatcaatg gcaaacaagc
    14281 agtagtaatc gctatggaag gaaaattcca aggtggcggt atcggtgaag tttccggtgc
    14341 aaaaatcgtt gctgcacttg aacatgtgtt agaagaaaat aaaaaaggaa atgagatctt
    14401 tccagtcatt gtactagata caggtggtgt tcgtttgcaa gaagcaaact acggactgtt
    14461 atctatttca gaaatcggaa atgtaatcat tgctctaaaa caatatgtac cagtcatcgg
    14521 tttgattcca ggacgtgtag gatcatttgg cggtatgtcg attacttcag cattaatgag
    14581 ttatttaatc gcaacaaaaa aagcgagagt cggtttgaat ggaccagaag ttattgaaca
    14641 agaagcaggc gttcgagaat ttgattctag tgacaaagac ttgatttgga atacattagg
    14701 atctcgtcaa cgggtagaat caaaaatcat tgatgaatta gtgactgata gtgtagaagc
    14761 aatcaaagct gctgtcatcg cagcaatgga cgaacgaaaa gacacgcatc gcagtgaaag
    14821 ctcagcattt tatttatcat tacttgattc tttagatctt tcaaaaccaa tggccattga
    14881 cgattacaat gaagcaattg ctaaccatca agcaaaatca attgacttac cagaagtagc
    14941 agaaggaaat gaaccagctg cagcaagtgt tggttacgat tggtttgcgg cactaactgg
    15001 catgaaaaat ccaacttctt ctattagtac tgtgtattat ggtaaatcaa gtaaattcat
    15061 agaagaagcc atcgtgacat caatcgtacc tgatcctaaa aacccgatgt accgtgtacg
    15121 aaacggtgaa gttggtttag ttgaaggttt ccgcatggga caaattttgg atcacgttta
    15181 cgaagaagat aaagacaaaa aagtgaaacg gccaatcatc gttgtgatcg atgtaccaag
    15241 ccaagcatac ggctataacg aagaattgat cggtatccat gtggctttgg ctaatagtgc
    15301 tgctgcttat gcaaaactcc gtcaagctgg acatccagtt attggattga ttgcaggaaa
    15361 tgctatttca ggtgcatttt tagcacatgg attacaatca agccgcttga ttgctttaaa
    15421 caataaagaa attaccgtcc aagcgatgtc taaagaaagt gctgcacgtg tgacaaaacg
    15481 ttcggttgct gaaatcgaag cagctgctga aaaagttcca gctatcgctt acgatatcaa
    15541 taattataca aaactaggag cgatctatca tttcctagaa gatatcaccg accaagctgc
    15601 ttcagaacaa aatgtcagca aagtcatcga agcaatcaat gaagccattc aagatgtccg
    15661 caatacaaat gacacgatgc tgacaaatcg atacacaaac gaaacagcca aaacattcgg
    15721 acgtgtcgaa acaaataaag tccgtgccaa aatggacgaa gaatgggatg cataaaaaat
    15781 gattcaagca catgatattg tcctttttga taaacatgaa atagaagggt tcaccttgcc
    15841 tgaatgggtc gttctaactg aaaagatgtt cggaacagtt cgtcgtgcgt cgacggatca
    15901 cgcagaaata ctaccgattg gtttacgtgg acctgcccgc aatcagagat ggtcaacaga
    15961 aatctcaaaa catgccgtca aacaagtcat tcatccgtgg gacatcatcg aacaagaatc
    16021 gtatagacaa gagaaaatca tggattatcc tgtttatcag caatttcatg ctgctagaaa
    16081 catattatca ggatgtaaat ggggaatcgg cggcagctta ggatttgaat tatcgactgg
    16141 gattccggca gtaaaagaaa ccagtgattt tgatttgctg ctgtacgcaa attcaccgat
    16201 tgaattgcca attcaggtaa ttcaatcgca tcctgctttt tttgagcaat tcgataccca
    16261 agtagtgact cggcaaggcg gtttttcact aaaagagtat ttgagagagc cagagaaaaa
    16321 aattctattg aaaacagtcg aaggacctaa attaaccaaa gaactttggt aataaagaaa
    16381 aagagagaga aagagatgtt tgaaaacaaa aaaatcaaaa tcaacgtaag tgacttccag
    16441 aaagtatcag ctgtaacggc tgttatgatg caaacgatat tgagctttgc tttggcaaac
    16501 agccacagcg caacaaatac agcttttctt ggtacctttt acgtcatcgt taaatacaca
    16561 gcaccaatgt ttatttttgc aattgtttat aatatggtaa aaacaagtca gcatttgtct
    16621 tattgggaat ttttgaaaga aaagtttttt gaactagtcg ttccgtatct tctatggacg
    16681 acggcttatc tttggctatt tccagcagtc caacaacaaa ctccgtatac ggatactgca
    16741 agctttttac tgaaatatgt cacaggtgat ggagcgcctc atttatggta taccgttatg
    16801 atgctgcaga tccaattgtt catgccgttt tttgtttggc tcggttacaa agtatttgct
    16861 aataaaaaat atgtatggcc agtattgatt ggttcaacga ttttatatgt cgcatggtat
    16921 ctgttctatc aaacacaagt attgactggg ccttatcatg acagctggta cttattagat
    16981 cggtttgttg ctagtttcat gatttatggg gtagccgcat ttgtttatca cgaaaaagtc
    17041 tatcaatatt tggacagagt acgttatctt ttcttacctg ttgccttagt tatcgcgttc
    17101 ttttctgtcc gttcattgct cgcacatcca ggagacttga gctttgcgaa tgcaccttac
    17161 ttgaatacga tccaaagttt atacagttta gtcatcatct ttgctgtctt cataggcgct
    17221 tccaaaatga ttgtaaacga ttcgccaaaa ttgccattgt tcaaatggtt gtctgtttat
    17281 gcttatcgta cgtatttagc caatgtattt gttttccaag tactgttatt attgtttaaa
    17341 gatagttggc tgcagttacc ttcaggagtc atgattcttg tagcttatct aatgacggca
    17401 agttgtgcat ttgctttaag ctggttactt catatcattt gggtagctat caaaaaaggt
    17461 ttcagcaagt aatagtagga agaaggagtg agtgtcatga taaatgacga acgaaaaaaa
    17521 gcagtgactc agcaactcgc tgcgttggta gaggaagcat tgatcgcaga agtaacatta
    17581 agtccaaaac ctggattagt ggatgcactg gatgcgggtg cacattccga tatggactat
    17641 gctttatttc ttaagtctgc taagacgcta actccttttt ttgaagaaat ggctcaaata
    17701 gcatggcatc atgctattga tcaggagtta agagaaagaa ttgctgagat tgggcgaaaa
    17761 gcggaaaagg caatgctgat tgctacaaat ggcatcaata cccataaagg agcgatttgg
    17821 gcaatggggc tattgatcag tgtcattgca caaaaaatca gtatggaaca gcctttttct
    17881 ttatcgaaaa tacttgatga tgtcagcaag cttgctgctt ttcctgatac aaaatatcgt
    17941 cataaaaaaa ccactcatgg tcatagggtg aagcaaaaat atggcgtaaa tggtgcatat
    18001 gaagaagcga tattagggta tccgcatatc aggcttgcaa ttcaagcagc taaggagcta
    18061 gataatgatt catatgtaca gaaacaaatg catatgttgt tagttttgat gagttcgcta
    18121 gatgacacat gcgtattgca tcgcagtgat cagaaaacgt tagtggaaat gcaaaaactt
    18181 gctcatgctg ctagtcaaag tgctttacca aatcgtccat ttacagcact gttgcacttt
    18241 tgtcaggaaa accaaatctc tcctggagga agcgcagatc tcttgtctgc aagcttgttc
    18301 atgctgaaga tagaggaatt atggactgcc gacacataca tttgtccgat gttactcaat
    18361 gactaaataa gacaaaattg ctaaaaaaac agtttatgca tctggttatt gactcttcct
    18421 tttagcaagg ctatcataaa aatagttttt tgtaataaaa gataacgagg aagacaatga
    18481 aaaaaatcaa ttatttagca atcattttgt tagtgggcct atgtttgttt gctctgccga
    18541 cccaaggatt cgctgtcgcg ggtggtcatg gtggaggtac atctagcggt cgaagttcgg
    18601 gcagaggttc atttggtggt cgttcatatg ggggcagtta ttttggcagt cgttcttacg
    18661 gcagagattc ttatgggtat tatggtccag gtacaggtcc ttctagttat gagcaaacgg
    18721 gatttcttgc attagctgtt ttgtcagtag gaggggtttc tgctttttct atcgtcaaga
    18781 ataaaagaca aaagaaaagg ctgaaagcgg agatattgtc ccgcatgtca ggaacggcta
    18841 aagaaaagaa agcacagttt ctggaaatcc aaaagatatt catgaagatc caatccgctt
    18901 gggaagaaga aactcctgaa aaagccagaa gctgttatac agacaagctg tttgccgaac
    18961 atcagaaagt tctgcaaaaa aacaaagcag acggtgtaag aaatcatact aaaaaaataa
    19021 ctattcagga agtaggaaat tatcgacaga tccacgaaaa tagttttgcc atccgaattg
    19081 atttcagatg ctgtgattat tcagtcgatc gaaaagatgg gcgattgatc tcaggaagta
    19141 agacacaacg acagtatttt tctcagatat ggtattttga tttttctgag aaagaaaaaa
    19201 gatggcaagc cgattttatt cagccgatta tgctggattg acaggaaata tttatgaacg
    19261 gagttgtgac aaaagccttg tcgcagctcc tttttctgaa taaacggtgt gccagagata
    19321 gcctatcggc aataaggcac gattttgtaa aatatgggaa gcgatttttc aaaatcgctt
    19381 cttatcgctc aaggctgaac atctctgtca caacctctcc actaaatggt gtttaaaagc
    19441 ttgcatctgc gataataaga acaaccaaac gaaaaatagg aagcactatt ttagtttagt
    19501 tgttcttatt acttgatgca ggacagcttt ttcacaacct cttttttgta tagatgggca
    19561 aaatttttgt aataataggc gtatgtcttt ttcgttgagg agatgaaaaa gcatatggct
    19621 tattttttgt ttgctttgct tactttcttt ccacttcaag tggcagaaat atctactttt
    19681 tattatgtaa gcggaaccat atacttgaaa tcagaaggag atcatttcta tgaaaacaag
    19741 aacaattgaa atacttcaca gaatcatgac agcccaatat ccagtaaaag caaaacatct
    19801 agcaagtgag ttcaatgtca gtcagcggac gatccgtcag gaagttcttg aagcgaatat
    19861 ctggttgagc agtaaaaagc ttccggagat ccgtacaatt cgcaacaaag gttttttgtt
    19921 gaagctggaa aaggaaaagc aagctcgatt agaaactgaa ataaaagatg tgaaagacgg
    19981 ctttttgaat cggcgcgaac gtatttttga tctagtttta gccgttgctt acagccgaat
    20041 cccgatccat ctaaacaaaa aagaagaaga atttcaaatc tctaaaagta cattagatga
    20101 agatatgaga cggttacgag caacactggt cagttatggg atagagatcg tcagttatgg
    20161 aaagcaagga ctggtttata aaggttcaga acgatctatc cgtacgatga tctatgattt
    20221 tatcaatcag aatctgggaa tgaccgactt ctttatgaat caagatgcac aattaacacc
    20281 ggctcaaaaa atctttctcc attattttcc tttggtggaa atcaaaaaaa tacaggagat
    20341 ctattccaat aagttgttga aacaagaaga tgatatttat aaaaaacaaa tcctgctttt
    20401 tacattgatc tggatccagc gaatcaggaa acatgaattt atctctgcca ttaattggaa
    20461 aaatacagaa ataaaagaca gtagcttttc tgcatttatt gaccacatga tcgaggaatt
    20521 tcaattggac aatgtcccgc aagtagaaag aaattatctt cgtttcacta ttgagacgtt
    20581 caatgctaga gatatctcca attcaatcga gtgggtacaa gctcaattac taacgatcca
    20641 attgatccaa tttgtagaga acgagacaca cgtacctttc catctaaagg aagaagcttt
    20701 attcgaaaat ctctataagc acatggctgc actgattgta agaatcaaga atcatatcca
    20761 agtcatgaat ccattgaaag aaaacatccg aacaaattat ttcccaatct accgcgcagt
    20821 cactcatttt gttcctacga tcgaagaagt aattggtggc aagatactgg aggacgagat
    20881 agcttttctt gtcatccact tttcgacgat cgccagtgta atcaagcgag acttgaatta
    20941 tgtttataaa tcagtcgttg tctgtaacca tgggatggct actggaaatc tgttagcaga
    21001 gaatctgaaa gaaaagtttc ctcagatcga gattactgca gttttaagtt caaaagaaat
    21061 cgaattagta gataaattgg atgtcaatct ggttttcagc acctttcaat tgaattatcg
    21121 aaataagccg cttttagttg tcgatccgat tttaactgag acgaatatct tggttataga
    21181 agactttttg aaaagaaatg aagaactaca gcggttagag cctaacagtc atgaatcgac
    21241 ggagttattc actcaaataa tagatgtcat gaaagaaagt ggtggccgag tagatcgaat
    21301 catctatggc aagttagaaa aattattcga aacaaacaat ttagaaatca acaaaaggga
    21361 gatccagcct atgttaaaag atattttgac ggacagcaat atcatgatca aagaaaaagc
    21421 cactacttgg gaggaatcga ttgaactagc tgctagacca ttggttaaag aaaacattgt
    21481 cgaagagcgc tatgtcgaag cgatgattcg tgcagtgaat gattacggac cgtatatcgt
    21541 gattggcaaa cacatggcac tagcccatgc ccgaccagaa gatggggtca ataaattagg
    21601 agtcagcgta gctacgattg agcaaccaat cgatttcggt aatcctgaaa tggatccagt
    21661 aaagattatt ttttgtttgg cagcagtaga ctcctactcg catctgaaca tcatgaaaga
    21721 actaattgaa ttgatcaaca acgaagagaa gttggatcga ttgattgaat gcacaaatat
    21781 cgaagatttc aaacaactgc ttttttcagt agttgagaaa taataaatga tacgggggag
    21841 aacaaagatg aacaaattga acattttatt cgtatgcggt gcaggtttag gaagtagttt
    21901 tgcagcacag atgtctgctg aggatgtttt gaacaaatta ggagtagaag ccaaactgga
    21961 ccattgtgat atttcttccg cagtttccat gaaaccagat gtgatcatca cagcagaaaa
    22021 ttttcgttcg cagttcgaaa agttcgcagt tgatcctaaa acggcgatga tctatttgaa
    22081 aaatattgtt tcaaaaaatg aaattgaaga aaaaatcacc ccagttctgc aagataaggg
    22141 agttatctaa atacaaaaag aaaagagggt aaaaa
//
LOCUS       DAZAID010000048        22160 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000048 DAZAID010000000
VERSION     DAZAID010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.048"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..500
                     /locus_tag="ACG9BE_001647"
     CDS             156..500
                     /locus_tag="ACG9BE_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFN3032390.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            501..920
                     /locus_tag="ACG9BE_001648"
     CDS             501..920
                     /locus_tag="ACG9BE_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFN3032391.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1012..1362)
                     /locus_tag="ACG9BE_001649"
     CDS             complement(1012..1362)
                     /locus_tag="ACG9BE_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032392.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1328..2659)
                     /locus_tag="ACG9BE_001650"
     CDS             complement(1328..2659)
                     /locus_tag="ACG9BE_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFN3032393.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2914..3480
                     /locus_tag="ACG9BE_001651"
     CDS             2914..3480
                     /locus_tag="ACG9BE_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3032394.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3625..3712
                     /locus_tag="ACG9BE_001652"
     tRNA            3625..3712
                     /locus_tag="ACG9BE_001652"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3657..3659,aa:Ser,seq:cga)
     gene            4048..5022
                     /gene="bsh"
                     /locus_tag="ACG9BE_001653"
     CDS             4048..5022
                     /gene="bsh"
                     /locus_tag="ACG9BE_001653"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFN3032395.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            5347..7242
                     /gene="asnB"
                     /locus_tag="ACG9BE_001654"
     CDS             5347..7242
                     /gene="asnB"
                     /locus_tag="ACG9BE_001654"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFN3032396.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7437..7883)
                     /locus_tag="ACG9BE_001655"
     CDS             complement(7437..7883)
                     /locus_tag="ACG9BE_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFN3032397.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8111..8260
                     /gene="rpmG"
                     /locus_tag="ACG9BE_001656"
     CDS             8111..8260
                     /gene="rpmG"
                     /locus_tag="ACG9BE_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN3032398.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8348..8866
                     /locus_tag="ACG9BE_001657"
     CDS             8348..8866
                     /locus_tag="ACG9BE_001657"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFN3032399.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8943..9899)
                     /locus_tag="ACG9BE_001658"
     CDS             complement(8943..9899)
                     /locus_tag="ACG9BE_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3032400.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10065..10871
                     /locus_tag="ACG9BE_001659"
     CDS             10065..10871
                     /locus_tag="ACG9BE_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3032401.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10868..11857
                     /locus_tag="ACG9BE_001660"
     CDS             10868..11857
                     /locus_tag="ACG9BE_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFN3032402.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11854..12858
                     /locus_tag="ACG9BE_001661"
     CDS             11854..12858
                     /locus_tag="ACG9BE_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFN3032403.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            12990..13532
                     /locus_tag="ACG9BE_001662"
     CDS             12990..13532
                     /locus_tag="ACG9BE_001662"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFN3032404.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13552..14250
                     /locus_tag="ACG9BE_001663"
     CDS             13552..14250
                     /locus_tag="ACG9BE_001663"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFN3032405.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14271..14483
                     /locus_tag="ACG9BE_001664"
     CDS             14271..14483
                     /locus_tag="ACG9BE_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFN3032406.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14483..15445
                     /locus_tag="ACG9BE_001665"
     CDS             14483..15445
                     /locus_tag="ACG9BE_001665"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFN3032407.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15463..15858
                     /locus_tag="ACG9BE_001666"
     CDS             15463..15858
                     /locus_tag="ACG9BE_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN3032408.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16021..16386
                     /locus_tag="ACG9BE_001667"
     CDS             16021..16386
                     /locus_tag="ACG9BE_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFN3032409.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16383..18194
                     /locus_tag="ACG9BE_001668"
     CDS             16383..18194
                     /locus_tag="ACG9BE_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFN3032410.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18256..18423)
                     /locus_tag="ACG9BE_001669"
     CDS             complement(18256..18423)
                     /locus_tag="ACG9BE_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFN3032411.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18672..19661
                     /gene="galE"
                     /locus_tag="ACG9BE_001670"
     CDS             18672..19661
                     /gene="galE"
                     /locus_tag="ACG9BE_001670"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFN3032412.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19673..21157
                     /gene="galT"
                     /locus_tag="ACG9BE_001671"
     CDS             19673..21157
                     /gene="galT"
                     /locus_tag="ACG9BE_001671"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFN3032413.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21181..>22160
                     /locus_tag="ACG9BE_001672"
     CDS             21181..>22160
                     /locus_tag="ACG9BE_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3032414.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         7178 a         3800 c         4599 g         6583 t
ORIGIN      
        1 caactatgct taaaaagcat atcgactgaa taaactaagc attggacagg ctgaaagact
       61 acatttaaaa tgaaatgcga agaaacaata gaaaataaag ttgccttaga accatagttg
      121 aatcacctaa cagtcgtgaa gggagaataa gagaaatggc agaaaaacaa accgcaggac
      181 gagatcaatt aggagatttt gcaccaaaat ttgcagaatt aaatgatgat gttttatttg
      241 gacaagtatg gtccagagaa gaattatctg ctcatcagcg tagtatcatc accatatctg
      301 cattaatttc aggaggaaat tttgaacaat tgccagctca tctgaagatt ggcaagcaaa
      361 atggcattac tagtgaagaa atagcagaaa tcattaccca tttagctttt tatgtagaat
      421 ggccaaaagc ttggtcagct tttagtttag caaaagaaat ctatttagaa gaaaatagcg
      481 aagataagaa agaggcgtaa atgatgacaa aacacgaaga aatacacaat ggcgtgattt
      541 ttccagcagg tgaaaaaaac gaagcttacg ctcaagtatt taccgggcaa agctatctga
      601 atatgttagt gaatgatccc gaagtttctg ttggtgtagg taatgtaact tttgaaccag
      661 gttgtcgcaa tcactggcat gtccatacag acggctatca aatcttactt gtgaccggag
      721 gagaaggctg gtatcaggaa gaaggaaagc cagcaagacg attaatggct ggagatgtcg
      781 tcgtaacgca caaagggatt aaacattggc atggtgcgac aaaagacagt tggtttgaac
      841 atttggcgat cactgctggt aattcagagt ttttagaacc tgtaacagat gaagtgtatg
      901 aaggattgga aaatcaataa tgtcaagctt gaacagctcc tgaaagtaga ttttaatcga
      961 actttcagga gctgcttttt tgattcagtc ttttataaat agaaaatagg gttaataata
     1021 ggaatgagaa gcacgtcgaa agatcttaat attgataatc ggctgggcat acatgatttc
     1081 cacgtcatca atcaaactac ctaacagatg ataatcttcc cgatcatgtc ggggattacc
     1141 taatagttct tgatagtaga tttccatact agggtcccga ggattgttca gcaaatcagc
     1201 aagtacatct aaatcttctg gctgagtttc tgccgtaccc tgtatctcta tgaaaagccc
     1261 ttcctcagca aattctactc caatacgtat ttcagttatt tcatgggaat ataaataagt
     1321 aagaagctca tcgatgattc tttttgataa ttctttttcc aagtgtcttt ttttcctttc
     1381 tgacagcggt aaagttatct agtatcggta caaggatacc tgcaacgaaa ccagttgaaa
     1441 aaccactgtt atatagattt agtcctccgt gcaaagtgct gacgttatgg acaagagcaa
     1501 tatgtagcac accagctatt acaccgtaaa aactgccgta aaaaccgctt atgggcgcaa
     1561 gacctgtccc aaacacagct gcaacaagca tgccagtact ggtgatctca tgaaaagttc
     1621 caaacaaaga agcaataaat attccgacta gtatcggaaa actgtttttt aaatgacaac
     1681 caaaagcact gaaacccacc gcagataaaa tagcaccgat gacaggtcca tttaactgac
     1741 cgccaaccag caaaacataa gaagacagca tcagaccaac taagcccatg ttgatcaaag
     1801 tagcaccaat accagaatta gcaatgaaat cagttgtcaa ttttccagat gaatcgaaaa
     1861 tttctcgaat ccccgaaagc cgaaaggaat tataatagaa accaattccg actgtcagcg
     1921 aaaaaaagcc aaaaataatc caaatgagag gaaagtgata ttcattgaaa accaatgtat
     1981 tttcgacgat tgaatagctg aataacctca aaacagcagt aaaagtcatc gcaatcaacc
     2041 cagaggtgaa tccaatatta taaagtgtga atccacgatg gaaaactaaa gtttgagcag
     2101 ctaagggcgg aagaagtata ccaattgcta tgccagctaa attccccaat aaaataccta
     2161 tcagtaaagg aaaacgcata ccaaaagtga tataactaac gaccggtgat aatacgctac
     2221 caaacagtcc gatcatgatg tactgagaaa aaggtttatt gacaaataca gcgtatagat
     2281 acacacctaa aatgattgag atagaattat aaaggttttt gccaaaaaaa gagaaaccgg
     2341 taactgttag aatcgaagca aataagggac cgtttaaaat caccttgtgc cgataagcta
     2401 aaagcacaga aagtaaggtc aaaatcccta catttatgaa tgcgcttccg aaaacaccta
     2461 aagcaaaata atctgtcaaa agattgcttg gcgaggtaag aatagttagc aatccactcc
     2521 aattatcagt aggagtatta gaaagcaagc caataaaaag aaaaaaaaca cctgtaaaac
     2581 caagataaaa atactttctt ttctgtgaaa cgattcgttt ttgaggatga atataaataa
     2641 cgtcagtttc gttttccatt acacaccttc tttttcattt tctttaacta taacttgaaa
     2701 tgaaagattt ggacaactat catttaggtc aaaactaaaa atgtaaggga acccattgaa
     2761 tttaaccaaa tttttttaat tttgcaaaaa aaagttaaag aagactgaaa aaaatttaaa
     2821 atataacgtt ttgagtaaaa tgtttgcaaa aaatgctaaa aatagggtac aataaaacaa
     2881 catatactgc agtaaagaag tgggggagtt ttaatgagaa gaaaagtcta taccaaggat
     2941 caaattctaa aagctgctta tgatgttgtt gcaaaggaag ggtttaaagg atttactgcg
     3001 agaaatattg ctaaaaagat ggggatttcg acacagccga tttatctgga attcaaaaac
     3061 atggaagatt taaaaaatac gttgctggat tctatctttg aacaactaaa agaagatgtt
     3121 ttccctattg aacgtactgg tgataaaatc attgatctcg gtttgaacta tatctacttt
     3181 gcaaaaaagc atcatagtct ttacatggca ttgtacattg atgaacatgg ggacggaaag
     3241 aaaatgcatg atttttcata caattatttc cgtgaaatag ttaaagaaga tgaaagatac
     3301 aatcaattat cggatgaata tgtcgatgct ctgcatactg gtacatgggt agcagtgact
     3361 ggcttagcgt cattgatgtc atcaaacatc atgcatccaa cagaagaaca aatcattgca
     3421 ctgatcaaac ggacaattga ctcgatttta gcattagata atcctgaaat aatcagatag
     3481 tgttctatca gtatgaattt gatcggtaag tccgttggct gcaaactgtc aatggacttt
     3541 tttgctaaaa aaagcgattt ctgttattta tctattagaa ataggcttga catttcgtga
     3601 aaaaagttta aaaatagtac ttacggaagg ttggcagagt ggtaatgcac cggactcgaa
     3661 atccggcgaa ccggctaata ccggcgcgca ggttcaaatc ctgtaccttc cttatatcaa
     3721 cgattagagg ttgtgaagaa gctgtcctgc atcaagtaat aagaacggcc aaacaaaaat
     3781 agcttttcct attttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
     3841 cgtttgttcg gaataagggg ctgtgacaag acttttgtca cagcccctta ttgttgtact
     3901 tttaacctac aatatttttt gattccccct gagtactatt ttttataaat gctagtaaat
     3961 caatcttctt tggccttttt tttgataaaa aaaaatacta gatgtaagat aaaagtgtaa
     4021 aaacgatttg ttttaggagg gaaaacgatg tgtaccgcaa tcacttatgc aacgaaagac
     4081 cattattttg gaaggaattt tgattatgag atgtcttata atgaagtagt cactattact
     4141 ccaagaaatt atcgtttcga ttttagaaag gtaaaaaatt tagataaaca ttatgcaatg
     4201 ataggaatag cagctggggt atccaactat ccactctatt atgaggctac aaatgaaaaa
     4261 gggttgagta tggcaggact gaattttcct ggcaatgctg attataaaga attacaagaa
     4321 ggaaaagata acgttgcacc atttgagttt attccatgga tactagggca atgctcaacc
     4381 atagacgaag ctaaagaatt attggctact atcaatctag tcaatatcga ttttagtgaa
     4441 aaactaccct tgtcaccttt acactggtta ttagctgata aagaaaaatc gatagtaatt
     4501 gaaagtatga aagatggact tcatttatat gataatcctg tcggtgtgtt gacaaataat
     4561 ccgccatttg attatcaatt atttaatctg aacaattatc gctcgctatc aaatggaaca
     4621 ccagaaaatc atttttcaaa ccagatcagc ttgaatgttt atagtcgtgg gatgggagga
     4681 ttgggcttac ctggagatct ctcttctgtt tctcgatttg taaaagccac atttactaaa
     4741 atgaatgctg catcaggtga ttcagaatct gaaagtatca gtcaattttt tcatatacta
     4801 ggctcagtcg agcaacaaaa aggagtatgc gatactggag aaggaaaata cgaatatacg
     4861 atttattctt cttgctgtaa cgttgataaa gggatttatt attatcgtac gtatgaagac
     4921 agtcaaatta ctgctgtaaa tataaataaa gaagacttgg atcgtcatga attggtcagt
     4981 tatccagtca taaaagagca acagataaac tatgtcaatt aattaatgaa tagatccgtt
     5041 tggtatcata ttttctgata cagtcataat tagacacgat ctttaggtcg tgtctttttt
     5101 tgctgttttt tttggaaata gcatccacta atattttttt aacacgaaga gaataaggta
     5161 gcctcaaaaa agaaaacatg gtaaattaga tattgggact ttattttctg taaaaacaga
     5221 aactttcatc ttttgttttc acgtgacgaa attgtaagag agaaaatatt cgtccgctat
     5281 ttattctatg ataagatgca tatgaatgaa ataaacagta attttatatt aggatgtgaa
     5341 agttaaatgt gcggtattgt aggttttgtt aacgacaagg acaacaaaaa aacaattatt
     5401 aatacaatga tggatcgaat cgttcaccgt ggacctaata gttcaggaga atatatcgat
     5461 aaacatgttg ctttaggatt cagaagatta agtattattg acttagaggg cggtactcag
     5521 cctatttaca atgaagatcg gacaaagatc attattttta atggcgaaat ctataattac
     5581 cagcctttga gagaagaatt gattgcagct ggccacgttt ttcaaacaca tgcagataca
     5641 gaagtccttt tgcatggtta cgaagaatgg ggaacagaat tattgcaaaa gatccgcgga
     5701 atgtttgctt ttgctatttg ggataatgaa aagaatgagt tattcggtgc aagagaccac
     5761 tttgggatca aaccatatta ttatgcagaa atgaacggaa catttatgtt tggttcagaa
     5821 atcaaaagtt ttctgcctca tcctgatttt aacaaagaat tgaacaaaga agcgttgaaa
     5881 ccttatatga ctttccaata ttctccatta aacggcgaaa cattcttcaa agatgtctat
     5941 cgtttgcctg aaggccatta ttatacgtat aaagatggaa aactagacat tcagcaatat
     6001 tgggatgcag attttgaaac aaaagaaacg cattcccgac aagaatggat cgaaaaaatc
     6061 gacgaaaccg ttcaagcttc tattgaagca catacggtca gtgatgtaga agttggttct
     6121 ttcttatcca gcggggttga ttccagttat gtaacctctg tattgaaacc agatcattct
     6181 ttctcaatcg ggtttgatga taaaacctat aatgaagcga ttgaagccag aaaactaacc
     6241 gaactattgg acttagataa tacagcagca gtgatcgacg gagacatgtc gttcaaagca
     6301 ttccctttga tccagtatca cttagatgaa cctgattcaa atccttcttg cgtacctttg
     6361 tactttttag caaatcttgc ttcacaaagt gtgcgggtag tccaatctgg agaaggggcg
     6421 gatgaactgt ttgcgggata ccaaacgtat ggattccata cgaattcaaa attcatccgt
     6481 gtgattgctc aaggattgaa aaaattacca aaaggaacac gctataattt aggacgtaaa
     6541 atcggaaaaa tgaaaaattt ccatggacgg atccatttat atgaatcact tgctcctgca
     6601 aaagaatatt ttatcggtca tgctcgagtg ttcgaagaat cagaagcatc agaagtgtta
     6661 acaccaaaat atcaaacagc accatctgtg gatgaaatca tgactgttca ttacgagaaa
     6721 acagaaggaa tcaaagatga agtcaataag atgcaatatg tcgatcttca tcaatggatg
     6781 ccaaaagata tcttgttaaa ggcagataaa ctttctatgg caagttcttt ggaagtccga
     6841 gtacctttat tggatatcga agtcatgaaa ttggctcaac aaataccaag taagttttta
     6901 ttgaatcaaa acaacacaaa agatattttc agacaagctg cgaacaaaca tcttccggaa
     6961 gaatggtcga atcgtgtgaa attaggattc cctgtgccaa tcaaagcttg gttgaaagaa
     7021 gaacacggct acgaacaagt caaagcatta ttcgaagcag attttgcaaa agaatttttt
     7081 gatcaagaaa aaatcatgaa gttgctagat gaccatcacg aaggacgtaa agaagagcaa
     7141 agaaaaatct ggacaatctt cagtttcttg acatggtata aagtctactt tgtagatgaa
     7201 acgattccgc aagcagaagc aatcgactat gtgactgttt gatgaaaatc aaatatcaat
     7261 aaaaaagagg ttttctccta ccgtttaaca ctgagtgata agaaacaatt tcaaaaaaca
     7321 gctcttcccg ttttatagat attatgactt atggttcgga gttggcagag gaacaccatt
     7381 gtttcgaaca taagggggtt gtgacacgat tttcgtcaca acccctttac atatgttcaa
     7441 taatcgattt ctttttcttt tgtcccatct tttttataaa tgaacaaagt tgattctttg
     7501 ttttgtgcta cttgctttcc tttttcgatt gcttcttttt ttgtatcaaa atggttgctt
     7561 gccttttttg cccctttaga tttcacgatc cattgttctt cctcatattc tacgatcaca
     7621 tctgaatcaa gcagttttcc agcacgagga ttcgtatcgt gttcatccgt cttgcttgga
     7681 tttttttctt tttcgaaagt tcgtttttct gattcactag catcttcata ccattttttt
     7741 gcttgatcaa tggcaattgg gatcgcacgg ttatcagggt acccttcatc taataatgca
     7801 tttgcgatgt cgattgcttt ttttcttgtc aatttatcta agtttttcat tgatgcagga
     7861 aagtccttca tgttccatgg catattagta tcccccttta ttaaaaaaca taatctatat
     7921 aaacaagtgt aagggtattt atacaaaaag aaaaagattt tgtttgagaa gtaaaaccgt
     7981 tgtatttatc gtcaagtagt tttacttaac aaaatcgtga aaaacactag caatctagaa
     8041 aaaatcatgg taagataatc aagtctgaga gtcatcttag aggattcttg taaaaatggg
     8101 agggaaaaac atgcgcgtaa acatcacttt agagtgtact tcttgtaaag aacgtaacta
     8161 tctaacaagc aaaaacaaac gtaacaatcc tgatcgtttg gaaaaacaaa aatattgccc
     8221 acgcgaacgc aaagttactt tgcaccgtga aacaaaataa tgtttttaaa gttctcaatt
     8281 ccttgctggt caaagaattg agactttttt tatctcaaaa catctaaaat cataaggagc
     8341 ggataatatg tttatttcca tgtgggcaca agataaaaat ggtctgatcg gcaaaaatgg
     8401 tttgttacca tggcgattgc caaatgatat gcgtttcttt cgcgaacata caatggatcg
     8461 aattttagtg atgggaagaa agacctatga aggaatgggg gatctttcac ttccttatcg
     8521 ccatatcatc gtgttgacaa cgcaaccaaa ttttcgcacg aaagaaaatg cggaagtaat
     8581 gcattcgatc gatgaacttt tgtcgtatag tcaaacgatc tcagaagata tctatgtttc
     8641 gggtggtagt cggatttttc aagaacttct tccttacact ggtcggatct ggcgaacatt
     8701 gatcgatagc acgtttgaag gcgatacgta tattgggaat attgattttt cagacttcgc
     8761 ccttatagag gagtatgagg ggataacgga tcaagaaaat ctatatgccc atcgttttca
     8821 aaaatgggaa agagtaaaaa atagggttgc ttcaaaagaa gaataaaatc tatgcgtcta
     8881 gtttgttaat aagaaaaaaa gcatctcacg actaatcgtg aagattcttt ttttagttaa
     8941 aatcaattta gtttttcttt gatatcattg accatttcct gactggcaat aggtccgtta
     9001 tatagccaac tagattcagg tccgaattgc agtacatgat tgtctgtcac tgctttaaga
     9061 ttcttccagc ttggttcatt gaacatggca gcatctttgt cactgttcac aaggatgatg
     9121 tagtcagcat ctaattcagc tagcttttca gaggaaacag cagaccagtc agaagtggct
     9181 tttttggaga cctcttccac tagatcagga acaccaaatt ccagatcgtc gtatacgatt
     9241 cttccacttg accgattttt gctgaccata aaggctgagt tgttggtcac ccacaacact
     9301 gcagctgtct tgttctcgat tttatctgct agttcttctc gagttttctt gatggatttt
     9361 tgataatcgg aaatgatttt ttctgcctct ttttcttttg ccaaagcttt gccgacatct
     9421 tttagttgtt cttcccatgt gacatctgtt ccgtttttta caacataagt tggcgcgatt
     9481 ttgctatatt gttcgtattt cccgccttcg acagtagcgg aagaagaaat caacagcaaa
     9541 tcaggctcaa agcttaacac tttctcataa ggaaggtcat aagaaatcgt tgggacatcc
     9601 tttaaatcat cttgcagata atcttgaatg gaaccactgc caacagtcca ctgggcgata
     9661 ggtttttccc ctagtgcaat cagatagtct tctaagtaac tgccgatgat ccgtttgggc
     9721 gcgtttggaa tctctacttt atgtccaagt gtatctgtga ctgtatgagt actattggat
     9781 gaagtcgtcg aattggcagt tgtagctgtt tctttatctg aagaggggga gcaagcgctc
     9841 agtactccag ccagcagcaa tatgctagat aatgtgaata cttttgtttt taatttcatt
     9901 tctttcctcc taacataatt gagaatcttt ctcattggtt attattcctg atatttcgaa
     9961 cgctgtcaac cgcttcattt ggctatagat atgaaagtag cgcttgacga aatgttgaaa
    10021 cagcgtcata attgagaatg attttcagcg aaaggatggg tgaaatgacg agattaagag
    10081 cagctgacat tgcagccggc tacgaaaaca aaaaaatcat tgaaaacatg tctgttacga
    10141 ttcctgaagg gaaaatcact agtttgattg ggccaaatgg aagtggaaaa tctactttat
    10201 tgaaaagttt tacccgtata ctacctgtga aatcaggaaa gatcattgtc aataactctg
    10261 atatctctac atacagttca aaagaacttg caaaaaaaat tacattatta gcacaaacta
    10321 gtgaacaccc tacaggtatg acagtagaag aagtagtatc ttacggacga tacccttatc
    10381 aaaaactgtt cagtggcatg gacacgcaag atcatgaagc aatcgagtgg gcgttggatg
    10441 ctacaggtct agctggattt cgtatgcggg atatcgcaac gctgtctggt ggacaaaccc
    10501 agcgtgtctg gatcgctatg gcgcttgctc aggaaacaga tatcttgatc ttggatgaac
    10561 cgacaacatt tttagatccg gcacaccaat tagagatcct acagttattg actgaaatca
    10621 atcaaatgaa acaaacaacg atcttgatgt cgattcatga cattaatcat gcttcacgtt
    10681 tttcagatta tttattagca atgaaaaacg gtaagttgat cacagaaggt acacctgaag
    10741 aagtgatcac aaaagagtgt atgaaaaaaa tatttgagat tgatgcaacg attgtcacgc
    10801 ttccaagaac aaataaacca gtttttctaa cctatgattt gttgccaaaa gagcaggagg
    10861 atcaattatg aggactatga agaaaaatag actcctgctt ttggtctgtt ttgttctttt
    10921 gattattttt tttgttgctc tttcaaatgg cgcagcgact gtgggaaaag cagatttttg
    10981 gcaggcgttg tttgattttg atagatcaaa acaaagtcac caaatcatac gaaatattcg
    11041 tttgcctcgt gtggcgggag catttcttgt agggagttgt tttgcattga gcggtgcatt
    11101 gatgcaagga gtgacaagaa atcctttagc agattcaggc ttactaggaa tcaacgcggg
    11161 tgcttcgttg gcaatggccc ttagttttgc tttttggcct aaagcttctg atttttctgt
    11221 gtttctaatg tcattggcag gttcttttgc tgtaactttg ctcgtatttg gttttttgca
    11281 gttttcgaaa atgggcatga atcctttaca gctgatttta gctggcgtag ctattagttc
    11341 tttttttact gctatcagcc aagcacttac ccaattcttt aatctgcagc aggatcttgc
    11401 tttttggttt gtaggcggag ctgccaatat cacatggaaa caattagcag tattgtttcc
    11461 tttatatata cttgctgttt tggtttcttt ttcattagga aaagcaatat ctttgattca
    11521 tttgagtgaa acacatgcat tagctcttgg aagtcatcct aaacgaacgc ggttgattgt
    11581 tttctgtcta gttgcagtgt tagcagccat tgccgtttcg ttagtcggac ctgtttcatt
    11641 tattgggctg ttggttccgc cagttgttcg cggaatggtt ggaacggact atcgttatgt
    11701 tctacctgtc tcttttttag ttggcggtgg attggttatg ttggcggatt ttacagcgag
    11761 gatgatcaat cccccttttg aaacaccttt tggattagtc attgcattga ttggcgttcc
    11821 gtttttactg atacaagtaa ggagggaaca acggtgaaaa aaatatattg gacgttctat
    11881 ttcttgcttt ttgtacttgt catcctgtct ataggtagtt taatgattgg tacgcctttt
    11941 atttacctgg atcagctttt tgccacgttc atagggaaag aatcatcttc tttggcgctg
    12001 attgttagag aatttcgagt tcctcgatta ctgatcagtt tattagcagg agcttgtctt
    12061 ggcgtttctg gttatatctt gcaaggagta acaagaaatg aattggcaga ttctagtata
    12121 ttgggcatta atgccggagc aggattttta gtgatgcttt atttaggttt cttttcgcac
    12181 tattcgctgc cgtttctatt gctagttgta gcctttctag gtggtgtttt agctgcttgc
    12241 tgtgtctatc ttgcggccta tgatcgaaat ggctttttgg gtatgaatcg aatcttattg
    12301 tccggtattg ccgttaatgc cggcctttct gcattaacat tgctatgcac gatccaatta
    12361 tctaaggaaa attatggatt tgtgaatgca tggttagcgg gatcaatctg gggtgcgagc
    12421 tggccttatg tttgggcatt gcttccatgg gcggttatct taattccgct aggaggactt
    12481 tatgcacaga ggattggatt gctatcattt ggtcaagaac gcgcgcaagg attaggcctt
    12541 aacgtggcta aaagtcaaaa aatccttttg ttgttagctg ttgctttggc aagcggtagt
    12601 gtagcggtga caggtagttt atcttttgta ggactactag ctcctcatgc tgcgaagcta
    12661 gtcgtacgta aagaaaataa ctggaccttt gttctgagcg gtttgttcgg ttccattttt
    12721 ctgcttagtg cagatatggt tgctagagtg attctaccaa gtggggaagt acctacagga
    12781 attttgattg ccctttttgg tgcgccatat tttctgtatg cactttttat cagacaaaaa
    12841 cacgcgctga acagttgaaa tttattaaaa aaggaaggcc aatcatttct aggtcttctt
    12901 tttattctat ttatgcattt atttgaaagt tcgattgcta tttttctttt tttcctatac
    12961 cattttatga gagaaggaag ggtgagtttt tggaccaatt gaaagtaaga ttacgccgga
    13021 aaggaatcga aaagctgaaa aatctggcca aaagtggcaa gaaaaaggaa aaagaacagc
    13081 gaatctataa acagctattc gccagcgagt actggaagtc agcaaaagtg atagggacga
    13141 ctttatcgaa tgaatttgaa ctggatacac agccggttat ccaacgagca gaaaaagaac
    13201 aaaagaaaat agttgtacct aaaactttgc cgaaacgtca gatggcgttt tatgaaatgg
    13261 atgaacagtc cgttcttgta cgttcgagtt ttggtgttct tgagccacaa tcagagaagc
    13321 tctatttgcc ggagatgatc gatctcctca ttgtaccagg tattatctac aatagagccg
    13381 gttatcgaat cggttttggc ggtggttatt acgaccggta tttaaataat tttcataaaa
    13441 aaacatgtag ccttgttttt cgtgaacaat tgaatgaaca atggcaaccg gagagctttg
    13501 atcagaagat ccaacaattg tttattgact aaaatgaaat gggaggtttt catgaattat
    13561 caacagcaaa taaaattgcg tgcatggtta agacgaccgt tatggaccta tgctttttta
    13621 gggatacaga cagcggtctt cctcatgatg gaattatttc cgcgacttga gatcccttat
    13681 tatgccggaa tgtatggtcc ttatcttgtt cacttcaatg aatggtggcg tttagtgaca
    13741 ccaatcttca tccattttgg actgatgcat ttcgtaatga actcactgat actgtatttt
    13801 atggggcagc aaatcgaggc aatctatggc cattggcgtt ttttcttgat ttatatgttc
    13861 agcgggataa tgggaaatac agcaagcttt gcttttaacg aagcgaatgt tctgtctggt
    13921 ggtgcgagta cttcgatatt cggtttgttc ggtgcgttgt ttatcttagg gtttcatttt
    13981 aaacataacc cagcgatcca gcagctagtc agacattttc ttttatttat tgtgttgaca
    14041 tttgtttttg gtcttctgga tacctctgta gatgtttggg gacatgtcgg tgggctaatt
    14101 ggcggattag tgttagggaa tgttttgggt ctaccgaaac aacagacaag ctattcgata
    14161 caccaaagga ttctttctac actagttttt gtatttcttt ttgtaatatg tattttactg
    14221 ggtttaaaaa aatatggttt acttgtataa tatgaaggaa gtgcttaaag atggagagct
    14281 tatatgatgt acagcagctg ttcaaaaaat ttggcatctt tatctatgtg ggtgcacgca
    14341 tctatgacat tgaattaatg actatcgaat tgcgtaatct gtatgatagc cgtttgattg
    14401 atcatgatac atacatgaca gcgtggcgca tattaaaaag agagcaccga attgaagaaa
    14461 gccgaaagaa aggaaatttg taatggacaa gaagattatt gggattgatt taggtggaac
    14521 aacagcaaaa tttgcaattt taacaccaga aggagagatt caacaaaaat ggagcatcga
    14581 taccaatatt ttggatgatg gtaagcatat cgttcctgaa atcattgaat caatcaacca
    14641 tcgtttgaat ttatataata tgaaagccga agactttatc ggaatcggca tgggtacccc
    14701 aggaagcgtg gacagcgaag ctggaacggt tattggtgcg tataacttaa actggacaga
    14761 agttcaattt gtgaaaaaac tgatcgaggc aggaacagga atcaaattcg ctatcgataa
    14821 tgatgcgaat gttgctgctt taggtgaacg atggaaaggt gctggagaaa acgatccaga
    14881 cgttgtattc gttactctag gaacaggtgt tggcggtggt atcgttgctg gcggaaattt
    14941 gattcacggc gtgagcggag caggcggaga gatcggccat atcactgtgg atcctgaagg
    15001 gttcgaatgt acttgcggta aacgcggatg tttagaaact gtgtctagcg ctactggtgt
    15061 ggtacgtcta ggtcgttact tggcagaaga atacgcagga gattcaaaac tgaaagcaat
    15121 gttagacaac ggtgaagaag taacaagtaa agatatcttt gaaatggctc aagaagacga
    15181 tccgtttgca ttgatggttg ttgatcgcgt atgcttctac ttaggtttag cttgcgccaa
    15241 cttggggaat acattgaatc cgtcaagtat tgtgttaggt ggaggagtat cggcagcagg
    15301 agaattttta cgtagccgtg tagagaaata ttttaaagaa tttactttcc cgcaagtaag
    15361 agaatcaaca aaaatcaaat tagcagagct tggtaatgaa gctggtgtca ttggtgctgc
    15421 ctcattagcc ttgaaatttg cttaaaaaaa cggaggcatt acatggtttt atgggtaatt
    15481 aatatcattt tgctattgat tgtgcttgca atcatctttt gggaagtcta tctacgagta
    15541 atggccaagc gttcagcaac gactttgaca gaagaagaat ttcgagaagg aatgagaaaa
    15601 gcacaagtaa tcgatgttcg cgaaaaagat gtgtttgatg ctgggcatat tttaggtgca
    15661 agaaacatcc cttacactgt tttgaaagat tcattaggat ctattcgaaa agaccagcct
    15721 gtctatattt acgatcaaaa gaaaagcttg agcatccgta cagcaaataa attacgtaaa
    15781 gcaggttatc aagagattta tttacttaag ggtggatatg acggatggtc tggaaaaatc
    15841 aaaagtaaaa aagtataaaa cagatgattg tatgacagct ttaaaaaccg aatcaatcat
    15901 tagattttga ccaagatcac atgaacggcg caatggatag ttgtgccgtt catttttttt
    15961 attttgtgct attatctaca tatataagct aagcgaaaag gcagaaagaa ggaaacaact
    16021 atgatcaaat taaaacgtgt ttaccttgat gctgaaaaag aggatggcta tagaatactc
    16081 gtggataggt tctggccacg aggaatttca aaagaaaaag cgcagttaga tctgtggttg
    16141 aaagaaattg cacctagtaa tgaagcacgc aaaacttttg accatgtacc agaacgattt
    16201 gcttcatttg caaagaaata ccagcaagaa ttgattttcg aagaagaaaa gaagcaagct
    16261 atttttaaac tcagtcaact tatgaaagaa catccagtca ttacttttgt ttatggtgca
    16321 aaaaatgaac atcagaacca tgctgtttta ttgaaaaaat ttatcgaaat ggaggcatgc
    16381 aaatgaaaat agggatcatt ggtgccggac ctagaggatt aagtatagca gaaaggattc
    16441 tccataataa tcaggacaga cattctcttg aactttttct tttcgatccc aatggtcctg
    16501 gaggtcgagt ttggcggttg aatcagccac cggagttatt aatgaattct gtttctcaac
    16561 aagtgacgct ttttacagat gaaacattgt ccagtggagg attggtaaca cagggaccca
    16621 acttgtttca atggagtaaa ggacctgcta cagattatat cgaacagacg gtaaccaaaa
    16681 ataagcaact aattatagaa gaaatcaatc gtctggaaaa agatcagcaa tctactcgat
    16741 gtttatacgg actctatcaa cgctggtttt tctcaaagtt gcaagaagaa tttccagctt
    16801 ctattcagtt tgtttattct ttagtaaaaa aagtcagcaa aagaaaagat ggttatcttt
    16861 tacagacaga tcagcaaagc tattctatcg atcagcttgt tttagcaagc ggtcactggg
    16921 aaaatgatga gacagaagaa gaaagagtgt gggtagacta cgcaaagaaa aatgatttac
    16981 tgtatcagct tccttctaat cctgcagatg tcgatctttc gaagataccg cctaaagaaa
    17041 atgtcttttt aagaggtcta ggcttaagtt tttttgatta tgtcggattg tttacagtat
    17101 caagagaagg gcgcttcgag aacgaaaaag gaaaacttgt ctatcatcca tcaggcaagg
    17161 aaccgattgt atacagtgga tcaagaaggg ggctccctta ttaccctcga ggaagaaacc
    17221 aaaagcaagg cggatcaatg gcatggccac ggttactaac aaaagaaaac ctggagcaat
    17281 ggcatcagtc gggatttttg actggcgaaa cattttttga ctatctgaaa aaagacgttg
    17341 agttgtttta ttacaagaaa atcatcgaag aatatcattt gccgattcca aagaaaaatt
    17401 ttgagcatga ttttctctcc ctctcttcag aggagtgtct cggaaagcat ccagcactaa
    17461 tcccttatag atgggattgg tcgtatttag aaattcctct tacctatcag gatgagtcct
    17521 ttgcagaagc tagccgagca tttattgaag ggcagataaa agaagcggaa aaaggcaatt
    17581 gcacaggagc atttgcttca acatttgatg cattaaaaga ctggcgtgat ccaatccggc
    17641 aggctattga atggcacgtt ttttctgcaa aggaatacaa agaaatacta tggggatggt
    17701 ttactcatct aaatagcttt ttaacgatcg gtccgccagt cattcggacg aaagaattag
    17761 cagcactgat agatgcgggt atttttcatt tgttcgaacc gccatttgat attcagcaaa
    17821 agcacaaccg ctatgttatt tctctacaag ataaagaaat cagcagtcgc tatttgattg
    17881 aagctcgatt gccacaaaca agcttttctc gttcaagtaa tcctgtcata aaaaatttga
    17941 aggaaaacaa acttgttcgc cctttttcat tttcagataa tacaggaagt tatcattcag
    18001 aagctataga tgttgagcga ggtacgaatc gcgtgattga tgcttcaggg gaaatccagt
    18061 ctgactttta ttgctatgga atacctgttg aaggtgtgga ttggctaact gcagcagtag
    18121 ctagacctta tacagatcca tggaatctga gacaggcaga taaaatagca caacttatat
    18181 tgactgacaa ataaaaatac gcatgatgac ttatctgttc tttcacaagt cttcatgcgt
    18241 gtttttaaat aattattaac gagaaacgcg tttgcgcatt gcttttttac ggttttcttc
    18301 gatcattgct tctttttcgt caattggatc gatcactgtt tttttcacag aagcgaccaa
    18361 accagcaacg gcagcggctg ttgccagact tcctactaat attccagata caaatttttt
    18421 cattgaatcc acctcattta ttatttgtta tatctattat ggaacaaaag agaaaaaaag
    18481 gctagtttta cgtttgtttg taaacctttg caaaaaatcc ttgaaaatgt ttactaaatg
    18541 ttttattata aatttaccat atgaatttac gaaattttac tttgtttgtt ttcaccataa
    18601 aagtaaactt cgttttttta aattatatat aatcaacaaa cacaatttta ctaatcaaag
    18661 gagtgttttt tatgacaatt ttagtactag gcggagccgg ttatatcggt tcgcacgctg
    18721 tagaccagct agttcaaaag gggtatcaag tagctgtggt agataatttg ctcacaggtc
    18781 acaaacaagc agtacatcca gatgctcatt tttatgaagg agacatccgg gataaagaat
    18841 ttctacgttc cgtatttgaa aaggaaccga ttgaaggagt aatccatttt gctgcaagct
    18901 ctttagtagg agaatctgtc gaaaaaccat tgatgtattt caacaacaac gtatacggta
    18961 tgcaaatctt acttgaagtc atgcacgagt tcaatgtgaa taaaatcgtg ttctcttcta
    19021 ctgcagcaac ttacggagaa ccaaaagagt ctccaatcac agaagataca ccagctaatc
    19081 ctaaaaatcc gtatggtgaa agtaaattga tgatggaaaa aatgatgaag tggtgtgacc
    19141 aagcatatgg catgcgctat gtcgctttgc gctacttcaa tgtagccgga gcaaaagcag
    19201 atgcatctat cggagaagat catacaccag aaacgcatct tgtcccaatc attttacaag
    19261 tagctcttgg tcaaagaaaa gcattggctg tttatggaga cgattatgat acaccagatg
    19321 gaacatgtat cagagattat gttcaagtag aagatttgat tgctgcacat attttagctt
    19381 tggaatactt gaaagaagga aatgaaagca acttcttcaa tttaggcagc agcaaaggat
    19441 actcagtcaa ggaaatgcta gaagcagcta gagaagtgac aggaaaagaa attccagcag
    19501 aaatagcacc aagaagagct ggagatccta gccgattagt cgcttcaagt gaaaaagcga
    19561 gagaggttct gggttggaaa cctgaataca cagatatcaa agcaattatc aaaacagcat
    19621 gggattggca tgtcagccat ccaaatggtt atgaagaata ggggaatgag atatgtcaat
    19681 cagtcaaacg atttgtgatt ttgccacact tgcgatcgaa gctggcggat ggatggaaat
    19741 ggaccgctta tatttacaga atcgtctttt agggatggta ggagaagatg ctctagaaag
    19801 cattgaagta agaccagtta aggaagaatc tgtagactta ttggatcagc ttgtcgctaa
    19861 ggcaagagaa aatggtgttc tttcagaatt gccagcagaa caagagattt ttgaagcgca
    19921 attaatggat tttctcacac cgccaccttc agtcgtcaat gcattctttg cacagcacta
    19981 ttcgaaaagc ccagaggaag caactgagta tttctttaat ttaagtaaga aaaatgacta
    20041 catcaaaaca agagccatcg caaaaaacat caaatttccg gcagagtcgg agtatggtga
    20101 actggaaatc acaatcaatt tatctaagcc agaaaaagat ccaaaacaaa tcgcagcaga
    20161 acgcgaggcg gtaaaagtag actatcctac ctgcatgctt tgcatggaaa acgaaggata
    20221 tagaggtcgc ttgaactatc cagcccgcac caatcaccgg attattcgaa tgaatctaga
    20281 cggtgaaagc tggggattcc aatattctcc ttatgcttat tacaatgaac attcgatcat
    20341 tttatcagaa caacaccgcc cgatgaaaat cacaaaggaa acatttgcta gactgcttcg
    20401 gattactgaa gtcttgcctc attactttgt tggttcgaat gctgacttgc cgattgttgg
    20461 aggatcgatt ctttctcatg atcattatca ggcaggacgt catgaatttc ctatggcaaa
    20521 agcaccgata gaaaaactta tcaaactagc agattatccg caagtcatcg caggagtcgt
    20581 gaaatggccg atgtctgtga tccgtttgca atcaacagag aaaaaattgt tgattgaagc
    20641 agctaactca gtattggaaa agtggaaagc atattcggat gaaacagtat ctatcagagc
    20701 tttttcagca gatggtacac cacaccatac gattacgcca attgcacgaa gaagggacaa
    20761 ttactttgag ttggaccttg tccttcgaga caacaatgtt tcagaagaac atcctgatgg
    20821 gatttttcat ccacataaag atgtccaaca tatcaaaaag gagaacattg ggttgatcga
    20881 agtaatgggg cttgctgttt taccacctcg tttgaaacca gaactagtag aagtagagca
    20941 tttcttatta ggagaagaaa accagatggc tgaatatcat cgtccgtggg cagaacaatt
    21001 aaaaaaagaa catcctgatt tgacgaaaga tacggtcacc gatgtcgtac aaaaatctgt
    21061 aggtcaggtt tttgcacgtg tactggaaga cgctggagta ttcaagcgag atgaaaaagg
    21121 acaggcagcg tttgaacgat ttactgcaat tttataaacg taaaaaagga ggagagacaa
    21181 atggcaacaa ttaaagacat tgcgcagttg gcaggagttt ctcctgctac tgtttcaaga
    21241 gttctcaact atgatcctga tctttccgtt ggcttagcca caaagcaaaa aatttttgaa
    21301 gcagcagaac gcttaaatta tacgaaacat cgtaaaaatg cagcaaatac taagcaaacg
    21361 cttcgcctga tccagtggta cgacgatacg gaagagcttg aagatcttta ttatttatct
    21421 attcgtctag gaatcgaaaa aaaggcagaa gaagcaaacg ttactttatt gaaagaaacg
    21481 tgggattcca ttagtgatct tccgacagat ggaacgatcg cactaggaaa attcgacgaa
    21541 gctcagttat tagaaatgaa aggatcccaa aatgccttgc ttttggtaga ttctgacggc
    21601 acttctcaag gaatcgactc tctagttgtc gactttaagt caagtgttca aaaagtcatt
    21661 gaacattttg tctcccataa agcgcaacat atcgctatgc ttgctggaac agaatatacg
    21721 aagaaaaacc atcagcggct aaaagatccc agaattatgg catttcaaca agttttggca
    21781 gaaaaaggct acgacgaagg tccaattatc gaagcagatt tttctgtcga gtcaggatat
    21841 caagcagtga agcagtattt aaaaacgaat ccgcatgttc ctgatgcgct ttttgcagct
    21901 aatgatgcat tagcgattgg cgcactgaaa gcgattcaag aaaaaggttt agtggtacca
    21961 gaagatattt ctgtgatggg tttcaatgat gtcagtgtag cgaaatatgt aacgccctct
    22021 ctttctactg taaaagttta taccgaatgg atgggagagc tagcagtgga gacgattctt
    22081 caattaagta attccaaagc accggtttca agaaagatta ttgtggaaac agagttgatt
    22141 attcgagaat caacgaaata
//
LOCUS       DAZAID010000049        21969 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000049 DAZAID010000000
VERSION     DAZAID010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.049"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACG9BE_001673"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACG9BE_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFN3032415.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACG9BE_001674"
     CDS             complement(2020..2922)
                     /locus_tag="ACG9BE_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN3032416.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACG9BE_001675"
     CDS             complement(2919..3611)
                     /locus_tag="ACG9BE_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFN3032417.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACG9BE_001676"
     CDS             complement(3654..4295)
                     /locus_tag="ACG9BE_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFN3032418.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACG9BE_001677"
     CDS             complement(4333..4647)
                     /locus_tag="ACG9BE_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032419.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG9BE_001678"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG9BE_001678"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFN3032420.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG9BE_001679"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG9BE_001679"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFN3032421.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACG9BE_001680"
     CDS             complement(5984..6916)
                     /locus_tag="ACG9BE_001680"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFN3032422.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACG9BE_001681"
     CDS             complement(6913..7689)
                     /locus_tag="ACG9BE_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFN3032423.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG9BE_001682"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG9BE_001682"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFN3032424.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPPTQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACG9BE_001683"
     CDS             complement(10933..12009)
                     /locus_tag="ACG9BE_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFN3032425.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACG9BE_001684"
     CDS             complement(12014..13294)
                     /locus_tag="ACG9BE_001684"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFN3032426.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACG9BE_001685"
     CDS             complement(13313..14239)
                     /locus_tag="ACG9BE_001685"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFN3032427.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACG9BE_001686"
     CDS             complement(14252..15535)
                     /locus_tag="ACG9BE_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFN3032428.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG9BE_001687"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG9BE_001687"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFN3032429.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACG9BE_001688"
     CDS             complement(16323..17234)
                     /locus_tag="ACG9BE_001688"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN3032430.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG9BE_001689"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG9BE_001689"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFN3032431.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACG9BE_001690"
     CDS             complement(17700..18398)
                     /locus_tag="ACG9BE_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFN3032432.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACG9BE_001691"
     CDS             complement(18385..18771)
                     /locus_tag="ACG9BE_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032433.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACG9BE_001692"
     CDS             complement(18918..20588)
                     /locus_tag="ACG9BE_001692"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFN3032434.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACG9BE_001693"
     CDS             20771..21004
                     /locus_tag="ACG9BE_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3032435.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACG9BE_001694"
     CDS             complement(21103..21534)
                     /locus_tag="ACG9BE_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFN3032436.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..>21969
                     /locus_tag="ACG9BE_001695"
     CDS             21613..>21969
                     /locus_tag="ACG9BE_001695"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFN3032437.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGA"
BASE COUNT         6205 a         4912 c         3883 g         6969 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagggg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagca
//
LOCUS       DAZAID010000050        20826 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000050 DAZAID010000000
VERSION     DAZAID010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.050"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACG9BE_001696"
     CDS             591..1280
                     /locus_tag="ACG9BE_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN3032438.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACG9BE_001697"
     CDS             1267..2475
                     /locus_tag="ACG9BE_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN3032439.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACG9BE_001698"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACG9BE_001698"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFN3032440.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG9BE_001699"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG9BE_001699"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFN3032441.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACG9BE_001700"
     CDS             complement(6578..7273)
                     /locus_tag="ACG9BE_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFN3032442.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG9BE_001701"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG9BE_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFN3032443.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACG9BE_001702"
     CDS             complement(8472..8663)
                     /locus_tag="ACG9BE_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFN3032444.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACG9BE_001703"
     CDS             complement(8684..9574)
                     /locus_tag="ACG9BE_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFN3032445.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACG9BE_001704"
     CDS             complement(9561..10328)
                     /locus_tag="ACG9BE_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFN3032446.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG9BE_001705"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG9BE_001705"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFN3032447.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG9BE_001706"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG9BE_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFN3032448.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG9BE_001707"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG9BE_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFN3032449.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACG9BE_001708"
     CDS             14899..15282
                     /locus_tag="ACG9BE_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032450.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG9BE_001709"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG9BE_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFN3032451.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACG9BE_001710"
     CDS             complement(16226..17044)
                     /locus_tag="ACG9BE_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFN3032452.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG9BE_001711"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG9BE_001711"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFN3032453.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG9BE_001712"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG9BE_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFN3032454.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACG9BE_001713"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACG9BE_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFN3032455.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..>20826
                     /gene="dnaN"
                     /locus_tag="ACG9BE_001714"
     CDS             19704..>20826
                     /gene="dnaN"
                     /locus_tag="ACG9BE_001714"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFN3032456.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVR"
BASE COUNT         5939 a         4249 c         3825 g         6813 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgta
//
LOCUS       DAZAID010000051        20799 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000051 DAZAID010000000
VERSION     DAZAID010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.051"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG9BE_001715"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG9BE_001715"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFN3032457.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACG9BE_001716"
     CDS             complement(2260..2511)
                     /locus_tag="ACG9BE_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFN3032458.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACG9BE_001717"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACG9BE_001717"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFN3032459.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACG9BE_001718"
     CDS             3461..3679
                     /locus_tag="ACG9BE_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032460.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="ACG9BE_001719"
     CDS             complement(3968..4480)
                     /locus_tag="ACG9BE_001719"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFN3032461.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ACG9BE_001720"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ACG9BE_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFN3032462.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="ACG9BE_001721"
     CDS             complement(6933..7376)
                     /locus_tag="ACG9BE_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFN3032463.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="ACG9BE_001722"
     CDS             complement(7377..8165)
                     /locus_tag="ACG9BE_001722"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN3032464.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /gene="rnz"
                     /locus_tag="ACG9BE_001723"
     CDS             complement(8162..9103)
                     /gene="rnz"
                     /locus_tag="ACG9BE_001723"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFN3032465.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="ACG9BE_001724"
     CDS             complement(9280..9867)
                     /locus_tag="ACG9BE_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFN3032466.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="ACG9BE_001725"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="ACG9BE_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFN3032467.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="ACG9BE_001726"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="ACG9BE_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFN3032468.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="ACG9BE_001727"
     CDS             complement(12784..13719)
                     /locus_tag="ACG9BE_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3032469.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVAPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13905..14864
                     /locus_tag="ACG9BE_001728"
     CDS             13905..14864
                     /locus_tag="ACG9BE_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFN3032470.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14950..16044)
                     /locus_tag="ACG9BE_001729"
     CDS             complement(14950..16044)
                     /locus_tag="ACG9BE_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFN3032471.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16233..16994
                     /locus_tag="ACG9BE_001730"
     CDS             16233..16994
                     /locus_tag="ACG9BE_001730"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN3032472.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17109..17549)
                     /gene="fabZ"
                     /locus_tag="ACG9BE_001731"
     CDS             complement(17109..17549)
                     /gene="fabZ"
                     /locus_tag="ACG9BE_001731"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFN3032473.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            17769..18524
                     /gene="fabI"
                     /locus_tag="ACG9BE_001732"
     CDS             17769..18524
                     /gene="fabI"
                     /locus_tag="ACG9BE_001732"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFN3032474.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(18634..19686)
                     /locus_tag="ACG9BE_001733"
     CDS             complement(18634..19686)
                     /locus_tag="ACG9BE_001733"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFN3032475.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            19812..20732
                     /locus_tag="ACG9BE_001734"
     CDS             19812..20732
                     /locus_tag="ACG9BE_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFN3032476.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         6000 a         4467 c         3686 g         6646 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcgcaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc
    13861 aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc
    13921 ttaccataga cgaactgaca atgatagaat catattatct tcaacataat aagccggttg
    13981 aaatcgctaa ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca
    14041 agcaaggcaa gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg
    14101 gtagaaaagt cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc
    14161 ttggttggac gcctgacgtc attatcgggc gaaaagaaag gtctgtttca tgcggtatga
    14221 gaacacttta tcgtttattt tctaaaggaa tatttgatat tgacacacta ccgatgaaag
    14281 gtaaaagaaa acccaatggc catcaggaaa aacggggaaa acaacagtat cagcgctcaa
    14341 tccatgatcg acctgataat tatcctgatt tcaattctga gtttggtcac cttgaaggtg
    14401 atacgatcgt tggcattcat cataaaagtg ccgtcattac tttagttgaa agattatcta
    14461 aagtcattat cacgattaaa cccaacggcc gtaaggcatt agatattgaa actgccctta
    14521 atcaatggtt ttctcgcttc cctaaaaact tctttaaatc tattacgttt gactgtggaa
    14581 aagaattttc taactggaaa gccattagta accaacatga tattgatata tattttgcgg
    14641 accctggaac accttctcaa cgcccattaa acgagaattc taacgggatt ctgcgtcgta
    14701 atggactgcc gaaatcaatg gattttagaa aagtgaatca gacatttatt tccagtgtca
    14761 gcaatcaacg taatcatatt ccaagaaaat cattgaatta cagaacacca atcgaaatat
    14821 ttttgagcta tgtacaagaa gcattttatt ctagcttaat ttgacaaatc ataacctata
    14881 aaaaaacaaa ataaaaaagc agaaataact ggctatttct agcggattat ttctgcaata
    14941 actaattatc tattttttat tttcaattaa tttaaagctt gaccatggtt tcaaaaagtc
    15001 aaggaggaat aattcgtcat cggagattct tcctacaaca ttgacacgtc cgtcgttttt
    15061 catatccttc aaggcgattt gtgtttctcc tttgtattga ccgtaccctt cattgtcaat
    15121 cagtacatcc ccatgatgca tatctcgcgt attatgaggc gggaaatctt tatctttgta
    15181 gtatacacga gtcatcgttg aacgaaggat atattctgag cgatcccctc gataactatg
    15241 aaggttatca aacaagcaga ttcgttcgtt ttctgtaata ccttcttccg tatctacttt
    15301 caatgttgga taatccgcgt taaatgcttc tgccatttct ttcaattctt cttcacttgc
    15361 gtacgcattt ccaatagaaa tatcatcaat caatcctgta aggattaaat gcttcacttg
    15421 tgtcgcaatt tcaagatctc ggtggtcttc cagcgtgcac aagccatctt gcgtaggcca
    15481 agggccaaac tcagcagatt gcgaattgac gaatgccatc gtattcaaat tatattttct
    15541 gaatttttct gaacaaaaaa caaagtgttc gtatcctaaa ccagagtatc gatgaggata
    15601 aaaattatga gaacctaata aattatccgt gtttggcgaa taactcatga tgttgtcaac
    15661 gtaattcgtg cccgaactca tattgatctc gattttgata ccaaaaggat tgcgcgtcat
    15721 ttttgcttct tctgctcctg tgaatccgat atctaagcgg accccgtagg cacccatctt
    15781 atggaaaaaa gataagtcat cgtaagaaat ctccaactgt tcaaataacg ctggattgat
    15841 atcaaccatt acttccatgc ctaggctatt cgcgtaatca actacttctt taaattctga
    15901 aagaactttt tctttatcat cattgatctg cagcaaactg gtaaaaaccc gtttataccc
    15961 atacttatga gctaaatcca agtacgcttt gtccttttca aatgttgacc gttctggata
    16021 aatcgagatt cctaattttc ccatattacc atttcctcct gcatcttttc gtttttgtat
    16081 ccacttacat tatagcaaac acaaagtaaa tggtctacac cttttctttt tcttttaaaa
    16141 taaatgataa attccaataa tttaagtagg tattttccct ctattttgtt atcattaaag
    16201 ataacaaaat agtaaattgg aaaggaacag ttatgaagaa aaataaagtt attttaagta
    16261 ttagtttagc agcagcattg ctgtttgctg ctggttgttc tgcaaacaac acgacttctt
    16321 ctagttcgtc tacagaaagt tcaaaaagta catctactgc ggaaagcacg actgccagta
    16381 gcgtcgattt gaacgcattg gaattaccac aattgacgaa agatgtcgcc gaaaatgaag
    16441 atctggtcga aatggtgacg aataaaggca caatcaaaat caaattattt cccgaacacg
    16501 caccaaaagc ggttgaaaac tttatgacac atgcaaaaga cggttactac aacggactga
    16561 ctttccatcg cgtcatcaaa gatttcatga tccaaggtgg cgatcctaaa ggcgatggca
    16621 ctggagggga aagcatttgg ggagaaggat ttgaaacaga gatcaacaac cacctttata
    16681 atataagagg tgcgttatct atggctcgct cacaagatcc taacagtaac ggcagccagt
    16741 tctttatcgt tcaaaataca gatgatatgc atgatggtct tttaaaagac gactatcctc
    16801 aagcaatcat cgacgcttat aaaaacggcg gttatccttc actagatggc cattatactg
    16861 tcttcggcca agtgattgaa ggaatggatg tcgtggatgc tattgcaaat atcgaaacag
    16921 atagttcaga caaaccaaaa gaagaagtaa aaatcgataa aatcaatata ctgcaagaag
    16981 caaaacaaaa ataaaaaaat aacgtctgaa tcctgcatag atgacaacgt atgctaggat
    17041 tcaggcgtct tctttttaca taaaaagcaa gtaagcttcc ttttgttttg ctatatcagg
    17101 ttttatagtc aagcattttg gttgacgatg aacgtgaact cgcactcaca gactttttta
    17161 tcatctacat atgctgcaga taacgctgta ccgactgatc ccttcatttt ggtgatttca
    17221 aaatgacatt tcagcacatc tcctggaaca actttttgac ggaatttggc cttattgatc
    17281 ccaccgatat aagctctttt tcctagaaac tcttctgatt ttaggataag tatcgaacct
    17341 gcttgtgcca gtgtttctag aatcaacgca cccggcatca ctggattgtc aggaaagtga
    17401 ccttggaaaa aatcttcatt gattgttaca ttttttgtcg caacgatctt ttttcctggt
    17461 tctaaagtat ctacataatc aatataacaa ataggataac gattaggaat caattccatt
    17521 acttcagtag cagataatac gtaagacata attaaactcc ttctttcagg caattgccat
    17581 tttcattact ttgactatca aagtatcata ttcctaaata tttagcaatc tttttttaat
    17641 ttaagaaaaa atagtttgac aatcaaaaca ttttgactaa taattaagaa atcaagaagt
    17701 tattttagct attattctct atttttttac gactatcaaa tttttatcag aaacaaagga
    17761 gagtcattat gtcatttcta gaaggaaaaa aaatcgtcat tatgggcgtc gctaacaaaa
    17821 aaagcattgc ttggggttgt gctgaagcaa tgttgaaaca aggggcagaa attatctata
    17881 cttaccaaaa tgaacgaatg aaaaaatcat tagtcaaatt ggtcggcgaa gaagcattca
    17941 ctgtagaatg tgatgtttct agcgacgaaa gcatcgaaaa agcaatgaat gccatcggtg
    18001 aatatgctgg ggagatccac ggccttgtcc atgcagttgc ttacgccaac aaagaagaat
    18061 tgtccggaaa tgtgtctgat atttccagag acggctatca attagctcaa gatatcagca
    18121 gctactcttt aattgctgta tcaaaatacg cacaaccgtt tcttgcacca aacagcggga
    18181 tcgttagcat gacttatcta ggttcagaac gcgcaatccc gaattataac atgatgggta
    18241 ttgcgaaagc ttctcttgaa gctgctatcc gttatttggc agctgagttc tcgcctaaag
    18301 gaattcgggt caatggaatc tcagctgggg caatcaaaac attagctgtc acaggtgtta
    18361 aagactatca aaaactgatc cagttatctg aatctcgtac gcctgatcag caaggtgtga
    18421 cgatcgaaga agtcggtaat gcttgtgctt tcttaatcag cccactttcc agcggaatca
    18481 tcggtgatat tatctacgtt gacaaaggtg tccatctttc ataaactttt gttgcattct
    18541 ttaaagtaaa acatataaaa atcaggaaaa aactttttaa agtctcttcc tgattttttc
    18601 tttttacgtt tttttcaact cattactttg aaattattcc acgacttcaa tccagccttc
    18661 tggtgcttct atgtctccaa attgaatacc tgtcaattca tcatacaacc gttttgttac
    18721 tggtcctact tccgtttcac tataaaatac atgaagattg ttattgtgat aaatcccacc
    18781 gattggagaa ataacagccg ctgtaccaca tgcaccagct tctgcaaatt gatctagatc
    18841 atcaatgaaa atatctcctt caattgcttg cagtcctagt cgttcttcag ctaactgcaa
    18901 caaggaatat ttcgtgatac ttggtaagat agagggcgat tgcggagtga tgaattttcc
    18961 gtcttttgta attccaaaga aatttgctgc acctacttct tcgattttcg tatgtgtggc
    19021 tggatcaaga taaacacaat ctgagtaatc cagttctttt gcttctgttc ctggaagtaa
    19081 actcgctgcg taatttccgc cgacttttgc tgcaccagtc cccttaggag cagcacgatc
    19141 gtaatcagat acaataaaat tcgttggttt tagtccacct ttgaaatacg ctcctactgg
    19201 cgtacagaag acgacaaata aatattctgg agctggatgg acaccaatgt tttcaccgac
    19261 cccaatcagt aaagggcgca agtataatgt agcgcctgtt ccatagggcg gcacaaaatc
    19321 ttcatttgct tttaccactt gcttgacagc atcaacgaac atcttttcag ggaccattgg
    19381 catcagcaat cgtctagcac tttgattcat ccgtcgacta ttttgttcag gtcgaaagag
    19441 attgaccttg ccgtcttttc gacgataagc ttttagacct tcaaaacatt gctgaccata
    19501 atgaagcgct ggagagcctt catgaatcgt caggtaatta tcttctgtca attctccttc
    19561 ttcccattgt ccgtctttcc atttggcaat aaaacgccaa ggagttttga tataagaaaa
    19621 acctagattg ttccaatcga tttgcatata cttaccacct tcgttatttt ctccacattt
    19681 tatcattatc catattattt gtcattttat tttcagcttt tttccttcat tatagttatt
    19741 tttgctattt ccttttttga aaacttcttg gtacaataca gaaagaatca aattttagaa
    19801 aagaggattt tatgttttca ttattagcaa ctgccggtac gacagactct tctggcgaga
    19861 cattgacaga tcaagctgtc agcaaagtaa atgcgtttca gcggttttgg gacgggattg
    19921 attgggacgc cattttggct gcctttatcc aaaaaagttt atatctgatt tttttgatcg
    19981 tccttttttg gattcttcac cgtgtgggaa aatatctgat tgataagtct tttaatcagt
    20041 attctaaaaa agccattcta aacgagagcc gcttaaagac tttacatagt cttttaaata
    20101 ctatttttca ttataccctt ggtttctttt ttatctatgc aattttatct gctatcggtg
    20161 tgccgatcgg atcacttttg gctggtgccg gaattgcagg tgtggcaatc ggtcttggtg
    20221 cccaagggtt catgagtgat gttattaccg gattttttat tatcatggaa caacagatgg
    20281 atgttgggga ctacatcaag ctagcaaact taacgattga aggaaccgtc gtatctgttg
    20341 gtatccgtac ccttcagctg aaagcaacgg atggtactgt ccatttcatc ccgaatcgaa
    20401 acatcaccac gatcagcaat ctctctcgtg caaatatgca agtactttta gatattcgga
    20461 ttgttcctga agaaggatat gacaaaatct atgagatcat tgatcgagta aatcgtacat
    20521 tagctgaaaa ataccaagaa gagctccaaa ctgaaccttc tatctttgga ttagtggatc
    20581 ttggaaacag caattttgcg attcgtacgg tctgctacgc attgaacgga aaacaatatg
    20641 ccttaaaaga agagttcttg agctcctatg tcaaagaatt gacagcttct ggtttcacga
    20701 ttccgaacag tccaattgct gtcggaaaat aatttggaca aacgatgcaa cgaatctcac
    20761 taaaaagagc gtgggacaaa agtaaagaac acttttgtc
//
LOCUS       DAZAID010000052        20283 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000052 DAZAID010000000
VERSION     DAZAID010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.052"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..3366
                     /locus_tag="ACG9BE_001735"
     CDS             <1..3366
                     /locus_tag="ACG9BE_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN3032477.1"
                     /translation="KVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAEVISDQSGK
                     KALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITLEISSNTKQ
                     TIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIEEESSKENE
                     NSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVLDHQGNKNG
                     TNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDVYLNARGNV
                     QKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINMGYVGYSSE
                     GYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPNGHKKVIVL
                     LTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQNNLSRRID
                     STFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSSDEKGDLYY
                     ESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVGTSPTTVTP
                     SISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTTFQPNIDTN
                     ELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAAAWKNGYIR
                     IIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIKELPVPGYD
                     SQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLTDHGDGTYS
                     LPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVTTSDDTIQL
                     TIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQTTNEKGLAS
                     FDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNVWTLTHQNR
                     LKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLKPGTYTLTE
                     TFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITNQAKVPLPE
                     TGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3369..4790
                     /locus_tag="ACG9BE_001736"
     CDS             3369..4790
                     /locus_tag="ACG9BE_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN3032478.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4787..6664
                     /locus_tag="ACG9BE_001737"
     CDS             4787..6664
                     /locus_tag="ACG9BE_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN3032479.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6756..7598
                     /locus_tag="ACG9BE_001738"
     CDS             6756..7598
                     /locus_tag="ACG9BE_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN3032480.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7750..8625)
                     /locus_tag="ACG9BE_001739"
     CDS             complement(7750..8625)
                     /locus_tag="ACG9BE_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN3032481.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            8832..9863
                     /locus_tag="ACG9BE_001740"
     CDS             8832..9863
                     /locus_tag="ACG9BE_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3032482.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            9952..10257
                     /locus_tag="ACG9BE_001741"
     CDS             9952..10257
                     /locus_tag="ACG9BE_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFN3032483.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10436..12451
                     /locus_tag="ACG9BE_001742"
     CDS             10436..12451
                     /locus_tag="ACG9BE_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN3032484.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12453..12737
                     /locus_tag="ACG9BE_001743"
     CDS             12453..12737
                     /locus_tag="ACG9BE_001743"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3032485.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            12763..14136
                     /locus_tag="ACG9BE_001744"
     CDS             12763..14136
                     /locus_tag="ACG9BE_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN3032486.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14219..16234)
                     /locus_tag="ACG9BE_001745"
     CDS             complement(14219..16234)
                     /locus_tag="ACG9BE_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFN3032487.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16256..17470)
                     /locus_tag="ACG9BE_001746"
     CDS             complement(16256..17470)
                     /locus_tag="ACG9BE_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3032488.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17691..18635
                     /locus_tag="ACG9BE_001747"
     CDS             17691..18635
                     /locus_tag="ACG9BE_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN3032489.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            18931..19875
                     /locus_tag="ACG9BE_001748"
     CDS             18931..19875
                     /locus_tag="ACG9BE_001748"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFN3032490.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         6924 a         3717 c         3862 g         5780 t
ORIGIN      
        1 aaagttattt tcatgctgat aatattgagt ttatcaaact ttgtgccatt atctgcaata
       61 gcagacacta cagatgatcc aacagtttta gaaacaattt cagctgaagt catttcggat
      121 cagtctggaa aaaaagcact gaacatcaag ctaaatgcga ataacaccag tgctgaaaag
      181 atagaaaaag aaattggtct agtcgaaaat tacttaagtg atgtggaaag aaaagaagga
      241 gatggctatg cttatcaggt aaatagcggg aaaattacgt tggaaatctc atcaaacact
      301 aaacaaacta tcgatctgag ttttccaatc gatccagcac tttaccacag ccaggcaaac
      361 aagctgatcg tcgataataa agaatatgac attattgatg agacagaaaa taagaaagat
      421 acagatgtgt cagtaccaaa gccagacgaa atagaagaag aatcatcaaa agaaaacgaa
      481 aattctgtca gcccatttac attgcctaca ttatccttgc cagctgtgag tgtgccatct
      541 aatcaaacga ttcctacaga atatacaaca gatgatcagg gcacttatcc taaagccagt
      601 tggcaaccta caggaaatac aaatgttctt gatcatcaag gcaataaaaa cggaacaaat
      661 caatgggatg gtataaattc ttggaatgga gatcctaatg atcggaccca ttcgtatatc
      721 gaatatggag gaaccggtaa tcaagcagac tatgcgatac gaaagtatgc aaaggaaaca
      781 agtacacccg gattgtttga tgtttatttg aatgctcgtg gaaatgtaca aaaagatatc
      841 acgcctcttg atctcgtatt ggtcgtagac tggtcaggaa gtatgaacga caataatcgg
      901 atcggtgaag taaagattgg tgtcgatcgt tttgtcgata ctttagcaga tagcggtatc
      961 acagacaaaa tcaatatggg atatgtcggc tactcaagcg aaggatatag ctacagtaac
     1021 ggtgcagtac agatgggttc atttgattca gtgaaaaatc aagtaaaatc cattacacct
     1081 tcacggacaa atggtggtac ttttacacaa aaagcactaa gagatgcagg aagcatgcta
     1141 tccgttccaa atggacataa aaaagtgatc gttttgctga cggatggtgt accaacattt
     1201 tcctataaag tacagcgggt acacgcacaa tcaagcagca attattacgg aactcagttt
     1261 tctaatacgc aagatcggcc gggaaatact tctctaatct caagaatcta tgatgcacct
     1321 gaccaaaaca atctatccag aagaatcgac agtacgttta tcgcaaccat cggagaagcg
     1381 atggcactca aagaacgagg aatcgaaata catggtcttg gcatccaact tcaaagcgat
     1441 ccggcagctg gtctctcaaa agcagaagta gagtctcgta tgcgacaaat ggtttcatca
     1501 gatgaaaaag gcgatcttta ctatgaatca gctgatcatg caacagatat ctctgaatac
     1561 ctagccaaaa aagctgtaca gatctcagca actgtaagca atggacaaat aaatgatcca
     1621 atcgcagaac cattcattta tcagcctggt acactttcag tcaagagtgt ggggacaagt
     1681 cctacaacgg tcactccatc tatttccata gaaggaaata ccatcaagag caatcagatc
     1741 tatttaggaa aagaccaaga aatccaaatc cattaccaag tgagaatcca aacagaaaat
     1801 gaggacttcc atccaaattt ctggtatcaa atgaacggca ggacaacttt ccagccaaac
     1861 attgatacca atgaattagc tgaattcggt ataccatctg ctaaagctcc cggagtcagt
     1921 cttcacatca aaaagttatg ggaagaattt gacaacaatc tagctgatcg tccagatcaa
     1981 gttacttttg agattcaacg ggaacatacg acaaatgctg cagcttggaa aaacggatat
     2041 attcgaatca ttaaaccagc taaagataca acaaatacgt gggaacgtgc agacattgac
     2101 aaattatctg caaatagcgg agaaagttat caagagatat tatcactacc tcaatacaat
     2161 aatcaaggtc aagcattcag ttaccaaaca atcaaagaat tacctgtacc aggatacgat
     2221 tctcaacaaa tagatgcaat gacatggaaa aatactaaac aattcacacc gttaaacttg
     2281 aaaataacga aaaattcctc tacaggtgaa aaggatctta ttggcgctgt tttcaaatta
     2341 acaggagatt ctattgatac tttactaaca gatcatggcg acggaaccta ttctcttcca
     2401 gaaaatgtca aattgcaaaa agaaatgacc tatacgctga cagaaacaaa agctccagaa
     2461 gggcatggat taagcaaaaa gactacttgg gaaatcaaga tcgcttctga tggtacggta
     2521 accattgatg gaaaaacagt cactacttcc gatgatacga tccagttgac tattgaaaat
     2581 ccttttgttg aagttcctgt agcagtacgt aagtatgcga tgcaagggac ggacaaagag
     2641 ataaatctta aaggagcagc attttcccta cagaaaaaag aagcaaatgg tacttatcag
     2701 ccaattgaca gccaaacaac gaatgaaaaa ggtcttgcca gttttgattc actcacacct
     2761 ggtaaatatc gagtcgttga aacagctggt cctgccggat atgatacttc gccgggaaat
     2821 tatgaattcc aaatcgataa atatggaaaa atcatttaca cgggaaaaaa taccgagatg
     2881 acaaataatg tatggacgct cactcatcaa aatcgactaa aagcgtttga tctaacggta
     2941 cacaaaaaag aagacaacgg acagacatta aaaggagcaa aattcagact gcagggacca
     3001 gaaatggact tagaatcgcc aaaagatgga caagaaacag atacctttct attcgaaaat
     3061 ttaaaacctg gaacttatac gctgaccgaa acttttacac cagaaggata ccaaggtcta
     3121 aaagagccag ttactatagt tatacacgaa gatgggtcaa ttcaagtgga tggacaagat
     3181 catgaatctg ttctgtcacc aggagccaaa aacaaccaga tttctttaga catcacgaat
     3241 caggcaaaag taccattacc tgaaacggga ggaattggcc gtttaggaat ctatctagta
     3301 gggatgattg gttgtgcgtt ttctatttgg tatctttttt tgaaaaaaga aagagggggc
     3361 agctaaatat gaaaaaactt ggttggctta gtatgtgtct cttcttgtta ctatttaaac
     3421 cagcttttac tcaggtagca acagaaacag aaacagaaat ggttcagatt actttacaca
     3481 aattgctttt cccaaacggg caactgccga aaaatcatcc aaatgacgga caagaaaaag
     3541 ctttattaca aacgtatcga ggattaaatg gtgtcacatt ccaagtttat gatgtcacag
     3601 attcttttta ccatctacgg gaaaagggca aaacggtaga agaagcacaa gcagagatcg
     3661 caaaaaacgg tgcgtcttcc ggtatgttta ccgcagaagc aacaactaca actcttaaca
     3721 acgaagatgg tatcgcttct ttttctctgg ccgctaaaga tcaagaaaaa agagataaag
     3781 cgtatctttt cattgaatcc aaagtaccag aagtcgtcaa agaaaaggca gagaatatgg
     3841 tagttgttct tcctgtacat ggacaaaaca atcaaaaact ttcaactatc catttgtatc
     3901 ctaaaaatga agaaaacgac taccctgatc caccttttga gaaggtatta gaagagccta
     3961 gaaatgattt tacgattggt gaaaaaatca cttattcctt gcatacgaca attcctgtaa
     4021 atatccttga ctatcaaaag ttcgaattgt cagatagtgc ggatgaagca ttaacgtttt
     4081 tacctaatag tttaacgatt tcatcgaatg gagaaaagct gacagaaggc tttgtcatac
     4141 acaagaaacc tcacggattt gatgttttat tttcgatccc ttcgttggaa aaatatgctg
     4201 gaaaaaaact gaccatttct tatcagatgc agctaagcag tacagcacag gcgaacaagg
     4261 aaatcaacaa caacggaaca ctggattttg gttttggtgt cagtacaaag aaagtctctg
     4321 tatatacagg gagtaagcaa tttgtcaaaa tcgagacaaa taaaccagat aaacgattag
     4381 ctggcgcagt attccttatt aaaaacaaag caggaaatta cctccagcaa acagccaacg
     4441 gatacaagtg gacaaagaac gaatcagatg cgcttcacct gatttccgat aaaaatggcg
     4501 ctttttcaat ttccgggttg aaaacaggaa gttatcgatt aaaagagatc gaagcacctt
     4561 ctggttatat tttaagtgaa acagaaattc cgtttaccat ttcaactttt ctttctgagg
     4621 ataaagaggc ggacagtata ttgaaagtag tcaataaaaa agaaaatagc cgtccatttc
     4681 ttccaaaaac aaacgaaacg aaaaatacac ttttaggcgt tgttggtatg gtattcgcaa
     4741 gctttgcaat ctggttgttt atcaaaaaaa gaacaggagt gaaaaaatga aaaatcataa
     4801 aaaaataaac gttatgttag gagtcctttt ccttatttta ccattactca caaacagctt
     4861 cggcgcaaaa aaagtgtttg cagaggagac agcagctcaa gtcatccttc ataaaaagaa
     4921 aatgactgat ttacccgatc ctttaatcca aaacagcggg aaagaaatga gcgaattcga
     4981 tcaataccaa ggattagccg atatttcatt ttcagtttat aacgtcactc aagaatttta
     5041 tgcgcaacga gataaaggag cgtccgtgga tgcagcaaaa caagcagtcc agtctttgac
     5101 tcctggtaca ccagttgctt caggaacgac agatgctgat ggaaatgtca ctttatcttt
     5161 acctaaaaaa caaaatggga aagatgcagt ctacacgatc aaagaagaac caaaagacgg
     5221 agtgtcagct gccgcaaaca tggttttagc tttccctgta tatgagatga tcaaacaagc
     5281 agatggctct tataaatacg ggacagaaga actagatact atccatctct accctaaaaa
     5341 tacagtcggt aatgatggaa cgttgaaagt tacaaaaatc ggtactgccg aaaacgaagc
     5401 actaaatgga gcagaattta ttatttctaa agaagaagga acaccaagcg tcaaaaaata
     5461 catccaaagt gtcacagatg gattgtacac ttggacaact gatcaaacca aagccaaaca
     5521 tttcattact ggtcattctt atgacatcgg caacaatgac tttgccgagg catctattga
     5581 aaaaggccag ttgatcgtta atcatttaga agttggaaaa tataatttag aagaagtaaa
     5641 agctcctgat aatgcggaaa tgattgaaaa gcaaacaatc acgccttttg agatcctggc
     5701 aaatagccaa acaccagtag aaaagaccat caaaaatgat acgtctaaag ttgataaaac
     5761 aacacctcaa ttgaatggaa aagatgtcgc aatcggtgaa aaaattcaat atgagatttc
     5821 tgtcaatatc ccattaggta tcgctgataa agaaggaacg caaaacaagt acacaacatt
     5881 caaacttatc gatactcatg acgctgcttt aacatttgat aatgattctt caggaacgta
     5941 tgcttatgcc ttatatgatg gaaataaaga aatcgaccca gtaaattatt ctgtcactga
     6001 gcaaacagac ggattcacgg tttcagttga tccgaattat attccttcat taactcctgg
     6061 cggtacattg aaattcgttt actatatgca tttgaacgaa aaagcagatc caaccaaagg
     6121 attttctaac caagcaaatg tcgataacgg gcatacaaat gatcaaacac caccgtcagt
     6181 cgatgtcgtt actgggggca aacgatttgt taaagtagat ggtgacgtta catcagacca
     6241 aacacttgct ggagcagaat tcgtcgttcg tgatcaagat agtgacacag cgaaatattt
     6301 atcgatcgac ccatccacaa aagccgtcag ctgggtatcg gcgaaagaat cagcaacggt
     6361 ttttacaacc acaagtaacg gtttaatcga tgtgacaggt ctaaaatatg gcacgtacta
     6421 tctggaagaa acgaaagcgc cagaaaaata tgttccatta acaaaccgtg tagcatttac
     6481 tatcgatgaa caatcttatg taacagcagg acagttgatt tctcctgaaa aaataccaaa
     6541 taaacacaaa ggtacacttc cttcaacagg cggtaaggga atctatgtgt atatcggtgc
     6601 aggagtagtc cttctactga ttgctggact gtactttgct agacgcaagc acagtcagat
     6661 ttagtctttt tgcaagaatt ggaaaaagga gcagcggatt acgcaaatgc tcctttttct
     6721 tttattatca aacagtcatc acaaggagaa cacaaatgaa accaaagaaa aggaaacttc
     6781 tagatctgct gatgcttttc ttgcttctct caggaatagg gatactagct tatccatttg
     6841 ttagcgacgc gctgaataat tatctggatc agcagatcat ctctcattat cagcaacaag
     6901 cggtcaaaga aaatgaagaa gtcatggcta aaatccaaga aaacatgaca aagaaaaacc
     6961 agcaattagc aaaagaaggt ggaaatccag gtgcagatcc ctttactaag aaaaaagaac
     7021 ctgcgaaaaa ggatagaacc tattttgaga aacatacgat cggtattttg acgatccctg
     7081 agatcaatgt ccatttgcca atttttgatg aaacgaatac tgtgctgcta gaaaaaggtg
     7141 cctcattatt ggaaggaacg tcttatccta cggggggaga aaatacacat gccgttattt
     7201 ccagccatcg aggactgcct caagcaaagt tgttcacaga cttaccgaaa ctgactttag
     7261 gtgattcttt ctatatcgaa atcaatggac ggacactcgc ttatcaagtc gatcagatca
     7321 agacggtaga accgactgat acaaaagatc tgagaatcga aaaagggcag gatttcgtga
     7381 cactcctaac ttgtacacct tatatggtca acagtcatcg cttacttgtc agagggcacc
     7441 gggttcctta tcatgcaaaa gaagttcaaa aagaagtaca gaaagtatcc gagaacaaac
     7501 gatttttcct gtatggattg ttagctgcat gtatactgat tttcctcctt cttatatatc
     7561 tttttcgtca atcgttcaaa ataagaaaca gaagttagct attcctcatt tctataatat
     7621 atctattcga atcgttccgt aaacaaacag gctgaggttt gaaaaagaaa acttcagcct
     7681 gtttttatga tgatatcaaa gagagcctat caaacactat ctttcttatt actaaatgag
     7741 gatcttttct taagttttcc gatattcttt aagaaggcct ttttgcatca attcttgttt
     7801 gtttttcaag ttgcggatat tctcttcaaa attttgagaa tatatcactg agaataacgt
     7861 atttaatacg taatcgaagg caatttgaga ggagaaagtc gctactttag caaaatcata
     7921 ttcctcctgc gatataacaa gacacatatc agcctgtttg gccatttctg aatgctgatt
     7981 tccggtaatc agcaaggtag gggcctctac atgcttcaaa taggtcatta ttcgttcata
     8041 atgatggatg cgaccgctgt agctaataaa aatggcgcaa tcctcgctag tcaaattggc
     8101 tgcattccaa gaagaatcac tgtattcttc agccatgatc aaaaacttat tcaacttgac
     8161 cattttattc tggaatttcc ttgccgtgat ttgagaatca ccttttgcaa aaagaaagat
     8221 tcgctgtgcc ttgttcaata ctttaccgat ttgatctaaa tcttcatgtt caatctgcaa
     8281 ttgtgctttt tttattgact ggatcgataa atccgccatt ttctttgcaa tttgctgagt
     8341 gctatcttca cttgaaaaag gaaaattagg atcaatcgtt tgattgaact gagcaatcaa
     8401 ttgatgaact tcccgcccca gctcgaaacg gaattcttta aatccgctga atcccatttt
     8461 tttgcataaa cgaatgatag ctgaatggga agtatacgta gcttttgcta attcttcaat
     8521 cgtcatgtag actgcagctt cgatattcct acggatgtaa tcgacaatcc gtttctctgt
     8581 cgtggtaaac aactcttgtt ttgttagttt ttcttcaata agcatgatct gacctccaaa
     8641 ttctatctta ctgataaaca cttggttccg ctactatctt tttgattttt atccttactt
     8701 tgaacattaa tttcaccaaa ccgaatgata acaagtgttt ttctgaactt ttttaccaga
     8761 atagctatta taaatacttt ctgcgctttt ctaggatata ctgaaaacat caataaaagg
     8821 aggcaccact catgcttaca atcgcttatc ttggcaatgg aaagagtaca aatcgctatc
     8881 atttgccttt ttcaagaaaa cttacgaaca aaatcaatat caaaaaaatt ttttccccat
     8941 ctgaaccagt gtggaaaaca ttcgaagaca tcgaatatac tgatcagctg tctgatcttt
     9001 gggatgatcc tgagatccaa ttagttgtca tcactacacc tagtcaattt cattatgaat
     9061 atggaaaaat ggcactcgaa catggtaaga atgtactagt agagaaacct tttgcagaaa
     9121 cgtcaaagga agcagaagag ttatttgcat tagcaaaaga aaaaggtcta tttatccaat
     9181 gttaccaaaa cagacggttc gattctgact tcttgacagt ccaaaaagta atcgaaagcg
     9241 gaaaattagg ggatatttta gaagtagaaa tgcattatga ttatttcaga ccggaagtac
     9301 cagaaaatac gcatgaattt tctactgcga acagctattt atatggacat gcttgtcata
     9361 cagtagatca agttctttct tatttcggcg aaccagatga tatcagctat gatgtacgtc
     9421 aacttttagg gaaaggacga atgaatgatt acttcgatct ggattttttc tatggaaata
     9481 tgaaaatttc gataaaatcc agttatttcc gcatcaaaga acgtccaagt ttcgtcgttt
     9541 acggaaaaaa agggatgttc gtgaaacaaa caaaagatcg acaagaagaa catctgaaaa
     9601 tgttttacat gcctgatcat gaagactttg gtatcgattt gcccgaacat tatggtgtat
     9661 tgacctatat agatgaacaa gggagttatc atgaagaaaa agtaatttcc gaggttggtg
     9721 attacagccg tgtctacgaa gggatttacg agacattagt taatggcgca gaaaaagtca
     9781 tcaaagatga agaaacaatc ttacaaatga aaattttgga agaaggaatc aaaagcattc
     9841 aagaatcaga aggaaaaata tgacatattt gtaagaatcg ggtttgtttt ttgacgcata
     9901 aactgtctta ttgtacgatg aaaaagtata gaactactag gaggaaccaa catgacaaat
     9961 tatgacggtg aaaaacacaa acgattaaga ggttcaatgg cccctaaaac agaagaagaa
    10021 gtggctttag caggaactaa ttctggtgaa acagaagaca aagaagaatc attagctggc
    10081 acaaactctg gtgaaacaga aaataaagaa acagctttat ctggttcaaa ttctgtagaa
    10141 gaggaaacaa agcctaatga gcccaatcac tttgaaaaag atatcgaaaa agcggttgaa
    10201 gatgtcaaag aaaaatttga agaactgacc tcaaaaaata aacatgatca tcattgatcg
    10261 gacgaaatca attcataaaa gcaggagaca tttggtcacc tgcttttttt ctgtatccat
    10321 tcgatagaaa tcgttcggta cagtcgtaaa aaaaatccaa ctgtaagttg taccaaacac
    10381 ccctcgattt aaaagcgcta ccatactaga atataagtag gaggtgaaca gcgctgtgaa
    10441 ctacaaaatt agttatctcc tgaaaacacc aagagaaatc agtctagaaa aagcagcaga
    10501 gatcatcgaa gtaaatacaa gcgaggcgat caaacaatta actccttttg ccgaatatgt
    10561 ggatgatacc cgtatccgta taaagaaatt ccgagcaatc gactgggcag aaaaattttt
    10621 ggatattact cgatttgaag ctgtgtatac cgaacaagag cgaagagatc ttctttattt
    10681 gatggtttat tcgactgcaa cgtctgtttc tgtttttcat tttcaagatt ttcttcaggt
    10741 ttctaaaggg acagtcctgt cagatatcaa aaagataaga aatgaattag aagtggaaga
    10801 ttgtcagctg tcgtatacta ggaaaactgg ttttaggctg ctagggtcag aagcaatatt
    10861 aagaagagaa gcaaaaaatc gcactgctcg tttgatgcag tcacttgctg gaagatttgg
    10921 gctcacgtat ttaaccgcaa ccctttccat tgattattac gccaaagtta gagatttgct
    10981 agataaaacg atttcactat cagatcttcg attcgcacca aatcgaatgg atgaattagc
    11041 ttattatttg agtttgacaa ccattcgaat tggaaaaaca tccatggaac tggtaaaaga
    11101 tgctgaactg ttggcttctc ttgcagtcac tgacattagt cgacaagtat tgactggcct
    11161 tggctattcc gaacatcaaa attcggagat tatatttttc tctctttgct tagcatcgat
    11221 cgttgaagga gatgtccagg agcctgcact tgattttttg ctggaatgtt cttcagaagt
    11281 catccatcgc atggaattcc tgatggccgt agaatttgct tcgtttcgtg aattattgat
    11341 gaatgtcttt tatcacttgg tccctgccta ttttcgtatt tcctattctt tttatttacc
    11401 taatgtgatg atcgatcaaa ttaagcatca atatgcttcc atctacgaaa tgacaagaaa
    11461 agcattactt ccgttagaaa gacgaatagg gaagagcatc cctgaagaag agattggttt
    11521 tttcactata ctatttggcg gagagatcag aaaagcggac gcagaagaaa gaaatagaaa
    11581 aataagagcc gtgatcgtct gtccaagtgg cattagctct tcattgatct taaaaagtga
    11641 acttcagcaa ttattcccga tgatcttttt tacagagact aattcagctg accgcttaca
    11701 cgaggttgac gaaacttctt acgatattat tttttctact gtacctctca cacttactga
    11761 taagaaaaag tctctctatg ttctgcatcc aatcatgact gacttggaaa aaaatcagct
    11821 gatcaatcaa gtacaaagag attggctgat accaggattt tcaatgcctt ctgcccaaga
    11881 attgttgtcg gcgctactgc cttatatcga attaaaagaa ggcgtagacg aagaaaagct
    11941 ctatcaagta ttaaatcgta aaatgaacaa acttttagaa aagaaggagg atcaacgtcc
    12001 tatgctatct gaattattaa cagaagaaat gatccaatta tcggatcaac caaaaaactg
    12061 gcaagaagca atcacatttg ccgcacagcc gctattatca gaaaataaaa tagaagaaag
    12121 ttatatcaaa gctatgatcg agcgcgtgga gcagtatggt gcgtttatcc atattggcga
    12181 tcacatcgca ttacctcatg ctcgtccaga agatggcgta aacgaagtag ggatgtcttt
    12241 gctgaaactt caacaacctg tggatctgct tgataatcca gcccatccca ttagtttgtt
    12301 catctgttta gctgctatcg ataatgaggc acatcttcgg gctttggcaa atttaacaaa
    12361 gctactttct aataaacaaa atcttcaaga tttattacac gcatcaacaa aagaagaaat
    12421 attagcgatc ataagaaaag gagacgaatg atatgaaaat ttttgcagta tgtcaaagtg
    12481 gattaggaac aagttttatg gtagagatga acatcaaaca agcacttgca gatcttggag
    12541 cagatgtgga cgagtttgaa gtaagtcata cggatgttgg aagtgtatct agcgatatgg
    12601 ctgattattt cttcattgag cattctttga aagatacgct aacttctata cctgatgaaa
    12661 agctcgtacc tttgcaatcg atcattgaca gcgaagaagt aaaagaaaaa gtaacgaaga
    12721 tcttgtcaaa agaataaaaa tgattcaagg gaggaactat caatggataa attgttgaat
    12781 ttgtttgtgt caatcgtcag tcagccctcc atcctcgttt ctttgattgc actgcttggt
    12841 ctcgttcttc agaagaagaa attttcagaa gtgatccaag gaactatcaa aacatttgtc
    12901 ggattcctag tactgatcgg cggtgctggt ttaatcagta attcactaac cccttttgct
    12961 tcgatgttcc aaacagctct gcatactcaa ggagtagttc ctagcaatga agcagtagtg
    13021 gcgattgctt tgcaacaata cggtacacct actgctttga tcatgctagt agggatgatc
    13081 gtgaatattt tacttgctag attcacaaga tataaattca tttttcttac aggtcaagca
    13141 atgctttatg tttcttgtct aacagcagtc attttagtaa gcgcggggtt tactgcaaat
    13201 ttgtggatga tcttattagg cggggtcttc gaaggaacac tattaactat tacgcctgct
    13261 ctttgtcaac cgtttatgaa gaacatcact ggaaacaatc aagttgcgtt agggcatacc
    13321 ggaaatatcg gttatgcttc tagtggatgg atcggaaagt tttttggaaa caaagaccgt
    13381 tctacagagg acataaagat tcctaaaagt ttcagctttt tgcgagattc tactgtatcg
    13441 atcatgctct tgatgtcgat cgtatatgtc gcacttgctt tgttagctgg tcctggattt
    13501 gttgaaagag aactaagcaa tggagacaac gctgtaatct ttgcactgat ccaagctgga
    13561 acattcacag ctgggtttgt tatcgtcctt caaggggttc gaatggtgtt ggcagaaatc
    13621 gttccagcat tccaagggat tgctaaaaaa ctcgtacccg attctaaacc agcattagat
    13681 gttcctatcg tttttccata tgcacctaat gctgtattaa ttggcttttt tgttagcttc
    13741 tttgtaggaa cactatccat gtttgctatg ataggcttgc aaactacagt tatcattcct
    13801 ggggtagtag gacatttctt ctgcggagct gcttctggtg tcttcggcaa ctcgactggc
    13861 ggtagaagag gggcggtatt aggttctgct ttcaatagct tattgattag ttggctgcca
    13921 ttatttatcc ttccagtatt aggagatctt aaaatcgctt cttctacatt tgccgatact
    13981 gattatctag taccaggact tgtacttggg aaactaggag aatacggtca aatagccttg
    14041 acagttggaa tcatcgcttt ctttttactt gtcgtattat ctagctttct ttttgccgga
    14101 aaaagaacaa acattgtttc taaagaacag aattaagcaa acttcaaaat aaaaattgct
    14161 tggacaatat cagaagaaga tttaaagtct tctgatatgt cgcaagcagt tttttgtttt
    14221 atgcatttat ttttgaatag cttgttaaga caagtggctg ttttgctaga agaggataac
    14281 ttttaccatt cattttcact gttccgcctg ttctatcaga agtaatcacg atcttatctt
    14341 ttgttttttc aagcgaaatc tttccatctt tgataggtag cgtacaattc agtgtttcaa
    14401 atgaatctag attaggttgt atcataaatt cttcataacc tggctttgtt ggatacaaac
    14461 cgataaaata gcgtcctaaa agatagattg ggcttgctcc ccatgcatgg cataagcttt
    14521 ttccataagg atctccatac atactgtact tttcatttcc tgtttgagta gggtcgtatt
    14581 cttcccagaa agttaccgca tcatgagata acatccctcc ccaataatca agaatcgttt
    14641 ggtagacgat attcgtctgt ccgagtttgc ataaagcatc ttgttcatag aatttgaaat
    14701 atggagtagt aatctgtgtg atagtgtcat tcaataagac attctttaag atcgattgct
    14761 gttggtcttt gttagcaaaa tcgaataaaa cagcgaaaat attcgcatga cgcgtcacat
    14821 ttcttctccc agattcatag caatcgataa aagcgccaag ttcttcatcc caaaagtagt
    14881 caaataggtt ttgtttcaat agttgatatt tttcttgata gagatgagta tgataatttt
    14941 cgttctctcc atatgtttca gctaagatat ctccgcaagt gatcatggaa tgataggctt
    15001 tcagtaataa gacttgttct gcagcaagcg ttccttcttt atccatttca gaccaatcaa
    15061 taaatatcca atcttttgct ctcccataaa taaaccctaa ttcattcgtt tgctccatca
    15121 agtatctcat taatgattgc atcttagggt agacgatgcg taaaaattca tagtctttcg
    15181 tcatcatata gtggttttcg ataccaatca accacaagat cgaatagtca acgatagtat
    15241 tcatatgctg cttgatttca ttttgtcctc ttaatgctaa tatcgttcgt ttattgattt
    15301 cctcgtcaaa aaaaagatat tgattgatca tgaatgattg atacgcatct ccagaccaaa
    15361 tccagcgatc tcgtttgata ccgtcgataa aaaacaaatc actacataac ttgaaggctt
    15421 cttctgatac tgaccagatc tgattcagta aagaagaatc gcttgaaaaa gcagcttgtt
    15481 tcgaaagaga taaaaattca tgaaaaactt gaagttgaac cattccagta gaaacctctg
    15541 gtataaacaa atagcgaaaa gcgcgtttac gagctttcat ttttcttgtc attcctattt
    15601 gcttatagta gcaataatcg atatccatag cttctacatc ggactctcca taacatagag
    15661 tgatagattc gatatggtca tccagagata ttagttctac acagccattc actgctcgcc
    15721 caaaatcgaa taagattcct ccgccaatct ccgtttcagt gactggatgg acgcattctt
    15781 tttgataata gatttgattt ggatcttttg cgatatcgac ataaagcgga ctccagccag
    15841 ctggatattc ttcaataaaa ttacttgctg tccagccaat atctgatttt acttcgtctc
    15901 cctcgataaa catcgctggt agccctgatg tatgtcctac aaagatacga atttttgctt
    15961 taccagctgg acaagtcagc cattttccac atggatgttt ttgtccatta atttctacat
    16021 acccaactcc ttggatcgtc actttaaaca tggaaggttg atcaagaaag taatatctct
    16081 taaacttgac gttccgatgg caatcatcca tgtaccagta cgctggccag tcaaaacctc
    16141 gttcttcccg ttggaaattt tgtagcagtc catggcggat ctcaaagtct ccaggatacc
    16201 aaagccatct atctttatgt ttttcatttg tcattttaca cgacctccat caaatttatt
    16261 ttgttgtcaa ttgttctttt atttcttcat ctactcttat cactgtcttt ctattcgtcc
    16321 tgtgtactac tagaaggcta ataagcgcgc taatcaacag caagatactg ccattgacga
    16381 aaacagcaag tgttttactc ccaagagaag caaaagagag aaagagcaaa ccatatggtg
    16441 caatgaaagt acctaaatta gcgccaacca ataagattga agtattgaaa ctctccgcgt
    16501 acttgggcgc ttggatactt acttggttga ataagtaagg aatgaataac gaaaaagaaa
    16561 ttcctgttag gatcgcacca aacccaatca catacaggtt tatcacagtg gcaatcaata
    16621 caaagccgat tcccatcagt agtaaagaaa gtgtaagcat atagcgatgt gcttttgtct
    16681 gaagccagcc aaaacaaact ccggcagcca tacttgccaa ttgcatgatt gttaagatat
    16741 tcgctgcatt agtttcgttt cctagagaat aagtcgtcag gactgtggct aatttaatcg
    16801 atacagcatt atataccacg atgaccagca agagaaaaat accataacta atcattttag
    16861 gattcgtttt gattacttgt cttgttgggg ctgtgccatg cgattgaagt tcagttgttt
    16921 caggtgtagg aacaaacaaa acaaacaaga caagaatagg taagctgatg gcgtagacta
    16981 aaaaggtata ttgccaaccg aactgtacta gctgtcctgc aacaaatgtc attaaagctg
    17041 caccaaatcc ttgaaatgca ctttgaaaac caatcataga ggctcgttca tctccgtgat
    17101 aaaaggcagc aatcatgctg actgccaaag aattgatcaa tccaaagcca atacctagtc
    17161 cagctcttga agctaagaca atactgtaac tttgtgagaa gagggggagg ataccacaaa
    17221 tcgatgcaat cactaatccg atgatcacag tttgtttttg tccgattttc ctagcaataa
    17281 aactactaat caaaacaaaa atagtgacca taaaagaggg agcagtcgtt aataattcaa
    17341 cagcagataa agaacgcgtg gcataagtag ctgacatact gggaattgca gacgtgactg
    17401 caccagcaga agtcaaaata agagagactg acagcagaga aaatttcatc atctttttat
    17461 tcataagcat gttcctttca tttttgtaag cgcttatcaa aaatatagta tgaagtggtt
    17521 attctctcaa tagcggaatc acaggaagtt catgtcccaa ccatagaaag tctttatgaa
    17581 accaaaaata gctttgcata taatagcagt acagcgtgaa ctaataagcg aaagaaaccg
    17641 cttacaaaaa ggagggagtc aattggaact agctgaatta gacatttttt ttgcgtaacg
    17701 aagaatcaat cgaaactctg cagcgtgaac aaggaatgaa tatcaatgat ttaaacttag
    17761 cttaccatct tcagacaccg ccaaaaatcc accgagggga tctatttcaa cagggaaata
    17821 ttcatatcag taaacaccgt cgatatgcgg aagttcccga gcatacacat gattttgttg
    17881 aaatcaatta tatgtatagc ggtacatgtt taaaaattat taatggagaa aggatagaga
    17941 tggaagaaag tgacgtggtg atcattgata aagaagctac tcaaagaatc gaatacacag
    18001 ggcaagagga tattttaatc aacatcttat taaaagaaga aaccttatct acagagatat
    18061 tgaatcattt ggcaagcaca aataatctac tttcttcttt tttgataaat gcatcaaaag
    18121 aaaaagggga acatacacaa ttcattcatt ttcgcggcgg aaaaacaagt cgtttgacgc
    18181 atttgattga aaatatgctg ttgatttatt ttgatacata cagtcataag atccgttcgt
    18241 taaatctcta tacatcacta ttactgatcg aattgactca tttattagaa caacaaagct
    18301 tttcatcagc agatggatgc gcagaaaatg agatgcttag agtattagat tatatcgata
    18361 agcattttac ttcttgtacg ttaaaagaaa cagcagctca ttttggctac aatccggtct
    18421 atcttagtaa taaattgaaa atgtattttg atgcttcctt tcaagaactg gttctaaaga
    18481 aaaaattcga ctttgcgttg gaactgattc aagaaacgag tttttcgttg gaagaaatcg
    18541 ctaatcgtat cggatataaa caaacgactt ctttattcaa attattcaag aagtattcaa
    18601 catgtactcc caatgaatgg agaaataaac attaagaaga aaatctttca ctaaagcata
    18661 tgaaggaaaa ttgttttagg tgcgattgct gtcttattat atcgttgcag cgaatttttt
    18721 tggatttatt ttcagttcta aacttttggt tgtattatca ggggatatag gtgtaaacat
    18781 ttgtcattta tctgttatgc gtattcagga attcgtttcc atcaaaaatt ataataacaa
    18841 ctattataat taacatgttg ataaaatgaa taaacaagac tatacttttc atgaaagtaa
    18901 caaatagtta tgaaaaggag ctgtttattc atgaaaaaaa caagtcgtaa agtagtaatc
    18961 gtcggtacag gttttgttgg aacaagtatc gcttatgcaa tgatcaacca aggaatctct
    19021 aatgaattag tattgatcga tgtgaatcaa gaaaaagcag aaggggaagc attggacctt
    19081 ttagatggta tggcttgggg cgatgaaaat gtggctgtat ggtcaggtgg ctatgaagaa
    19141 tgtaaagatg ctgatatcgt tgttatcaca gctggtatca accaaaaacc tggtcaatct
    19201 cgtttagatt tagttaaaac aaatgcgtca atcatgcgcc aaatcgtaaa agaaatcatg
    19261 ggatcaggat tcgacggtat tatcgttgtt gcttcaaacc cagtagatat tttgacatat
    19321 atcgcttgga acgaatctgg tctgccaaca tcacgtgtta tcggtactgg aacaacattg
    19381 gatacaactc gtttccgtaa agaaatcgca ttgaaattaa aagttgaccc acgcagcgtc
    19441 cacggctata tcttaggtga acatggtgat tcagaagttg ctgcatggtc acatacaact
    19501 gtcggtggta aaccagtgtt tgaaattgta gaaaaagacc atcgtattgc ccaagatgaa
    19561 ttagatgtga ttgctgataa agtccgtaat gctgcttatg agatcattga tcgtaaaaaa
    19621 gcaacttact atggtattgg tatgagtact gcacgtatcg taaaagctat cttgaataac
    19681 gaacaagctg tattgcctgt atctgcttat ttaactggtg aatacgacga aaaagatatc
    19741 tttacaggtg tgccatcaat cgttgacgaa aatggtgttc gtgaagttgt tgaattatca
    19801 atcaacgaag aagaaaaagc aatgttcagc aaatcaacaa gtgctttaag agaagtattg
    19861 aacactgtat tataagaaac aatgaatatg aactaaaaat cctaaaactg cttaacagct
    19921 gaaatagctg ttaagcagtt ttttattgat atctaatagg taagcaaata aatagaatgg
    19981 ttaaaattaa tcggtccgaa aatgtatccg tacatatgaa catatgaaat ttaatctgtt
    20041 gattacaata agcgaatcgt tatcaatgtt tatctgtggt taattaaaaa atccttccgc
    20101 aacggcaaat acaatgattc atatctaatt cgttcatatt tgattaactg aataaagaaa
    20161 ctggttttta tcattcaatt aaacgacaaa aaaagtaaga aaaactgcaa aattttttta
    20221 taattttgca taagcagatt ttgatggaaa aatgacgatt tttgataaaa aaccgttcga
    20281 gag
//
LOCUS       DAZAID010000053        19629 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000053 DAZAID010000000
VERSION     DAZAID010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.053"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG9BE_001749"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG9BE_001749"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFN3032491.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACG9BE_001750"
     CDS             complement(1172..1867)
                     /locus_tag="ACG9BE_001750"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFN3032492.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACG9BE_001751"
     CDS             complement(1880..2596)
                     /locus_tag="ACG9BE_001751"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFN3032493.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACG9BE_001752"
     CDS             2926..3534
                     /locus_tag="ACG9BE_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFN3032494.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACG9BE_001753"
     CDS             3859..4182
                     /locus_tag="ACG9BE_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFN3032495.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACG9BE_001754"
     CDS             4364..5734
                     /locus_tag="ACG9BE_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFN3032496.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACG9BE_001755"
     CDS             complement(5864..7165)
                     /locus_tag="ACG9BE_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFN3032497.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACG9BE_001756"
     CDS             complement(7519..9510)
                     /locus_tag="ACG9BE_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFN3032498.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACG9BE_001757"
     CDS             complement(9654..9920)
                     /locus_tag="ACG9BE_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN3032499.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACG9BE_001758"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACG9BE_001758"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFN3032500.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACG9BE_001759"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACG9BE_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFN3032501.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACG9BE_001760"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACG9BE_001760"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFN3032502.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACG9BE_001761"
     CDS             complement(14080..14601)
                     /locus_tag="ACG9BE_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFN3032503.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACG9BE_001762"
     CDS             complement(14604..15446)
                     /locus_tag="ACG9BE_001762"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFN3032504.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACG9BE_001763"
     CDS             complement(15439..16164)
                     /locus_tag="ACG9BE_001763"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFN3032505.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACG9BE_001764"
     CDS             16340..16636
                     /locus_tag="ACG9BE_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032506.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACG9BE_001765"
     CDS             17204..18796
                     /locus_tag="ACG9BE_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN3032507.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACG9BE_001766"
     CDS             18809..19549
                     /locus_tag="ACG9BE_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN3032508.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5617 a         4028 c         3724 g         6260 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaagtaaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgagatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttc
//
LOCUS       DAZAID010000054        19403 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000054 DAZAID010000000
VERSION     DAZAID010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.054"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..900
                     /gene="purC"
                     /locus_tag="ACG9BE_001767"
     CDS             172..900
                     /gene="purC"
                     /locus_tag="ACG9BE_001767"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFN3032509.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            900..1154
                     /gene="purS"
                     /locus_tag="ACG9BE_001768"
     CDS             900..1154
                     /gene="purS"
                     /locus_tag="ACG9BE_001768"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFN3032510.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1156..1827
                     /gene="purQ"
                     /locus_tag="ACG9BE_001769"
     CDS             1156..1827
                     /gene="purQ"
                     /locus_tag="ACG9BE_001769"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFN3032511.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1828..4050
                     /gene="purL"
                     /locus_tag="ACG9BE_001770"
     CDS             1828..4050
                     /gene="purL"
                     /locus_tag="ACG9BE_001770"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFN3032512.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4035..5474
                     /gene="purF"
                     /locus_tag="ACG9BE_001771"
     CDS             4035..5474
                     /gene="purF"
                     /locus_tag="ACG9BE_001771"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFN3032513.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5497..6549
                     /gene="purM"
                     /locus_tag="ACG9BE_001772"
     CDS             5497..6549
                     /gene="purM"
                     /locus_tag="ACG9BE_001772"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFN3032514.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6546..7124
                     /gene="purN"
                     /locus_tag="ACG9BE_001773"
     CDS             6546..7124
                     /gene="purN"
                     /locus_tag="ACG9BE_001773"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFN3032515.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7121..8656
                     /gene="purH"
                     /locus_tag="ACG9BE_001774"
     CDS             7121..8656
                     /gene="purH"
                     /locus_tag="ACG9BE_001774"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFN3032516.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8672..9946
                     /gene="purD"
                     /locus_tag="ACG9BE_001775"
     CDS             8672..9946
                     /gene="purD"
                     /locus_tag="ACG9BE_001775"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFN3032517.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10068..10982
                     /locus_tag="ACG9BE_001776"
     CDS             10068..10982
                     /locus_tag="ACG9BE_001776"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFN3032518.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11115..12680)
                     /locus_tag="ACG9BE_001777"
     CDS             complement(11115..12680)
                     /locus_tag="ACG9BE_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3032519.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12673..14124)
                     /locus_tag="ACG9BE_001778"
     CDS             complement(12673..14124)
                     /locus_tag="ACG9BE_001778"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3032520.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14138..15154)
                     /locus_tag="ACG9BE_001779"
     CDS             complement(14138..15154)
                     /locus_tag="ACG9BE_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3032521.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15374..16462
                     /gene="chvE"
                     /locus_tag="ACG9BE_001780"
     CDS             15374..16462
                     /gene="chvE"
                     /locus_tag="ACG9BE_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN3032522.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16516..18042
                     /gene="mmsA"
                     /locus_tag="ACG9BE_001781"
     CDS             16516..18042
                     /gene="mmsA"
                     /locus_tag="ACG9BE_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN3032523.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18044..19249
                     /gene="mmsB"
                     /locus_tag="ACG9BE_001782"
     CDS             18044..19249
                     /gene="mmsB"
                     /locus_tag="ACG9BE_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFN3032524.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
BASE COUNT         6204 a         3296 c         4444 g         5459 t
ORIGIN      
        1 gatggtataa gattttcgtt tctgataaaa cacgaacatt aaaagaatct aatgccttaa
       61 atgtacatat ttttgttgat ttataaaaag atgattgcta aaatttaaag tgaaaaaacg
      121 aacaaaaaac gatgtatttg cttttaatgt aaataaaaag gagagacgta catggagaag
      181 aaagaacttt tgtatgaagg aaaagccaaa aaaatatatc ttacttctga tgagaacttg
      241 atttggatag aatatttaga tcaagcaact gcgttgaatg gcgcgaggaa agaccagata
      301 agtggaaaag gagaattgaa taatcaaatc acaagtgcaa ttttccgtta tctgactcaa
      361 agaggaataa aaaatcattt tattgaacaa ttgtctaaaa ccgaacaatt agcaaaaaaa
      421 gtacaaatta tccctctgga agtggttatc cgaaatattg cagcaggaag tttctcaaaa
      481 cgtctaggtg ttcccgaagg taaagaattc atcaaaccaa tcattgaatt ctactataag
      541 gatgaccagt tggatgatcc atttatcaat gaagagcata tctatttctt acaacttgca
      601 aagccggaag aaatcagtga gctgaaagcc caagcgttgg ctattaatga aattttgacc
      661 gaattatttg ctaaggtcgg tttggtttta gtagatttca agctagaatt tggccggtcg
      721 aatggacaaa ttttgcttgc ggatgaagta acaccggata cttgccgatt atgggatgaa
      781 cgtacgaatg aacatatgga caaagacgtt taccgcagag atctaggtga tttgatccca
      841 gtctatcaag aagtatataa ccgtctatcg gaactaactg aagaaaagga gcgagtatga
      901 tgtatttcgt aaaagtatat gtaacctata aagattcagt gttagatcct caaggggagg
      961 cagtaaaaaa agcagtccat cgtatgggat atgaaacaat cgaagatatc cgaataggaa
     1021 aatactttga gatacaactt gctgatgcag aagaaccaat cgatacagtg attgaaacga
     1081 tttgtgacaa gctgcttgca aatgtaaata tggaaacata ccgatatgaa atcaaaaaaa
     1141 tggaggaagt ataacatgaa atttgcggtc atcgtttttc caggttcaaa ttgtgacgca
     1201 gatatgttat gggcagttcg cgagatcatg ggcgctgatg ccgaatttgt acggcatgat
     1261 gagtcctctt tagaaggatt tgatggggtt ttactgccgg gaggattctc ctatggtgac
     1321 tatttgagat gcggtgcgat tgctcggttt tctacaatca tggaagaagt gattcgtttt
     1381 gctgaagaag ggaaaccagt attcggtaca tgcaatggat tccagatatt gacagaagca
     1441 ggacttttgc caggtgtact gagacgaaat gagtctttgc attttatttg caaaactgta
     1501 ccattgaaag tcaaccccaa gacggccttt acttcagagt atgaagccga cgaaataatc
     1561 tatcttccag ttgctcatgg ggaaggaaat tatttctgtg acgaagaaac acttcaagaa
     1621 ctaaaaaaaa ataatcagat tgcctttact tataatgaca acatcaacgg cagcgtggaa
     1681 aacattgcag ggattacgaa caaagcaggg aatgtgttag gaatgatgcc tcatccagaa
     1741 cgtgcagtag aagacttgct aggatcagaa gatgggaaac ggttctttgc ttccatttta
     1801 aaaaacttcg gaaaggtgac cgtatagatg aaaacattga aagaaccgac agctgaagag
     1861 atcaagaaaa aaagaatcta tgccgaatgg ggattgactg atgaagaata tcgaatgatc
     1921 gaagaagata tcttaggaag aatgcctaat tatacggaaa ctggactgtt ttctgttatg
     1981 tggagcgaac attgttccta taaaaattct aaaccggttc tacgcaaatt tcctactagt
     2041 ggacctcatg tgttgcaagg ccctggagaa ggcgcaggca tcgtcgacat cggtgatgga
     2101 ctagcagttg tcttcaaagc agaaagtcac aatcatcctt cagccgtgga gccttatgaa
     2161 ggagctgcga caggagttgg cggaatcatc agagatattt tcagcatggg ggctcgtcca
     2221 attgcgttgc tagattcatt gaggttcggc gaattagata atccaagaac gaaatattta
     2281 ctagaagaag tagttgcagg tatcggcgga tacggcaatt gtatcggcat accgactgta
     2341 ggcggcgaaa ttgcatttga tccttgttat gaaggaaacc cattagtcaa tgcaatgtgc
     2401 gtaggtttga ttgaacacaa acatatccag aaagggcaag ccgctggcgt cggtaattcg
     2461 atcatgtatg taggtgcaaa aacaggtcgc gacggtatcc acggagcaac ttttgcctca
     2521 gaagaattta atcaagaaga agaacagcaa cgttctgctg tacaagtagg cgatcctttt
     2581 atggaaaagc tgctgcttga agcgtgtttg gaattaatct tgcatcattc ggattctcta
     2641 ataggtatac aggatatggg agcagcagga cttgtttcat ctagtgcgga aatggcttca
     2701 aaagctggct caggacttat attgacatta gatgaagtcc cacaaagaga aactgggatg
     2761 acaccttatg aaatgatgtt atcggaatca caagaacgga tgttgatttg tgttaaatca
     2821 ggagaggaag aaaaaatcca agaactattt cagaagtatg atctagatgc agtcactatc
     2881 ggtaaagtaa cagacgacgg gcaatatcgg ctatatcatc aaggagaaga agtagcaaat
     2941 ctgccagtcg atgcattagc tgaagatgca ccagtttatc ataaagaaat gaaagaacca
     3001 gctcgaatcg tagaatttca gcagttagct ccttatcagc cagttatcga agaacctgga
     3061 caagtattgc ttgatctgct gcagcaaccg acgattgctt caaaacgttc aatctacgaa
     3121 acttatgatt cacaagtaca aaccaataca gttgtccgtc caggaagcga tgctgctgtg
     3181 atgagggtta gagggacgaa taaagcgtta gctatgacca cagactgcaa cgcgcgctat
     3241 ctgtatttgg atccaaaaat cggaggacaa atagcagtgg cagaagctgc tagaaatatt
     3301 attgctagcg gtggtaagcc cttggcgatc accgactgcc tgaactatgg ttctcctgat
     3361 aaaccagaag ttttttggga attatcgact tcagctgacg gaattgctgc tgcgtgtgaa
     3421 acgttaggaa ctcctgttat ttcaggaaat gtctctttat ataatgaaac ggatggtcaa
     3481 gcaatctatc caacaccaat gattggtatg gtcggattga ttgaggatca taaacatatc
     3541 actactcagg aattcaaaaa atcaggggat ctaatctata tattaggtaa gacgtttgca
     3601 gattttgatg gcagtgaatt acaaaagatg cagcttggaa gaatcgaagg tgtgatccga
     3661 aactttgatc tatccattga aaagagaaac caagagttag tattgacagc catccaaaac
     3721 ggattgattg aaagtgctca tgactgttca gaaggtggct tggcaattgc actggcagaa
     3781 tcagctttca aacatcagct agggatatct gtacagtttg aattgtcctc tgctcaatta
     3841 tttgcagaaa ctcaatcaag atttgtgttg acagtcgcac cggaaaataa gacacgattc
     3901 gaagaaatga tgggagatgc tgctgtttta gcaggtaagg tgacggatga agcgataatc
     3961 gaaatttcag caactgacgg tcagatcaaa atagaaactg cagtagcaag aaagtgctgg
     4021 gaggatgcaa tcgtatgtct tatgaagtga agagtttaaa tgaagaatgc gggatcttcg
     4081 gtgtttgggg tcatccagac gccgctagtg tggcatactt cgggctccat agtcttcagc
     4141 atcgaggaca agaaggtgca ggtatcgtgt ccaatgatcg aggaaagctt aaggggcatc
     4201 gagatttggg gctgctttct gaggtattca aagatcaaag aaaattgcag cagttaacag
     4261 gagaagctgc aatcggacat gtacgttatg caacagcagg caatggaagt gtggacaata
     4321 ttcagccttt tctattcaaa tttcatgatc agtcgatcgg cttagcgcat aatggtaact
     4381 tgatcaatgc tcgttccctt agaaaagagt tagaggaaaa tggggcgatc ttccattcta
     4441 actccgatac ggagatattg atgcatttga tccgacgaag tgaagaaaca gattttttaa
     4501 ataaattaaa aagtgctttg aatgaagtca aaggtggctt tgcctattta ttaatgacag
     4561 aaacggcaat gattgccgct ttggacccaa atggttttcg tccattagca atcggacaaa
     4621 tgaaaaacgg agcatacgtg attgcctcgg aaacttgtgc attagaagtt attggggcac
     4681 gttttataag agatgtccag cctggtgaga tagtgatcat caatgacgaa ggaatccaga
     4741 ttgattgctt tacgaaggac acgcagcttt ctatttgttc gatggaatat atctattttg
     4801 ccagaccaga ttcaaacatc gcagggatca atgtgcatac tgcacggaaa aatatgggac
     4861 ggaatttagc gaaagaatcg ccagtaaaag cagatatggt cgttggcgta cctaattctt
     4921 ctttgtcagc cgcaagcggt tatgcggaag ctagtggaat cccttatgaa atcggattag
     4981 taaaaaatca atacattgcc cgaacattta tccagccaac gcctgagcta agagagcaag
     5041 gtgttcgaat gaaattatct gctgtacgag gagtagtgga aggtaaaaag gtgattatgg
     5101 tcgatgattc aattgttcgg ggtacgacaa gcagacggat cgtccatctt ttgaaagaag
     5161 caggggcaaa agaagtccat gtgaggattg cttctcctcc tttgaaatat ccatgttttt
     5221 acgggattga tattcaaacg cgtaaagaac tcattgcggc aaatcacacg attgatgaaa
     5281 tcaaagaatg tatcggtgca gattctttag gttttttaag tgaagagggt ctgatcgaat
     5341 cgatcggtct ggaaaaagat gctccgtatt caggattgtg tatggcttat ttcaacggag
     5401 attatccgac gccgttatat gactatgaag aaaaatatca ggaatcacta aaagaaaaag
     5461 tatcgttctt ttaaaagcag accaagaagg gagtatatga attcaatggc aaatgcatac
     5521 gcaaaagctg gcgtagatgt cgaagctgga tatgaagtag tagaacggat caagaagcat
     5581 gtcaaaaaaa cggaacgttt aggtatgatg ggcgctatag gaggattcgg cggttgtttt
     5641 gatttatcgg tctttcaatt aaaagagcca gtccttgtct cggggaccga tggcgtgggg
     5701 acgaaattag cagtggcaat cgaagcagac aaacacgata caatcgggat cgactgcgta
     5761 gcgatgtgtg tcaatgatat cttagcacaa ggcgcagagc cgttatattt cttagactac
     5821 ttagcaacgg ggaaaaatga tccgttacgt ttggaaaatg tagtaaaagg tgtagcagat
     5881 ggatgtgttc aaggaaatat ggcgctgatt ggcggagaaa cagctgaaat gcctggtatg
     5941 tactcaggta atgattatga tctagccgga tttgctgtag ggatagctga aaaatctcaa
     6001 ttattgacag gagaaacgat caacccagga gatgtgttgc ttggaatagc atctagcggg
     6061 attcattcaa atgggtactc tcttgttcgc aaaattttct ttgagcagca gcagttttct
     6121 ttatcatcaa aactgccaga attgaaaaaa acacttggcg aagaattgct tgagccaacg
     6181 aaaatctatg tttcttcctt gcttccgctg atcaaagaaa agaaaataaa aggagctgca
     6241 catatcactg gtggagggtt tgtagaaaat atcccgcgaa tgttaccaga atctatggct
     6301 gcacaaatca aacttggctc atggccgatt cttccaatat tcggctgttt acaaaaatat
     6361 ggtgaaattc ctgagatgga gatgtatgaa atcttcaata tgggcatcgg tatggtcctg
     6421 gcagtccaag aatcgaaagt aaaagaaata gtacacgaaa ttgagcaagc tggcgaaaaa
     6481 gtttatgaaa tcgggaaagt gatcgccaaa gaaaaacatc gtgtggtgtt tataggagaa
     6541 gaacgatgag aatcgctgtt tttgcttctg gaaacggaag caactttcaa gcgctagccg
     6601 attatctatc gaaaaaagga ttggaatctt cgattgactg gttgttttgt gatcagcctg
     6661 aagcatatgt tttgaaacgt gcaactgcgt tgagtgtacc ggcagattgt ttttcaccta
     6721 aagaatttga ttcaaaaaaa gagtatgaag aagcaatttt gcataaattg aaagaaaaaa
     6781 agatcgattt gatcgtatta gctggatata tgcgaatcat tggaccggta ttgctgaaaa
     6841 attatgataa aagaatcatc aatatccatc cgtctctttt acctgccttc cctggtttgc
     6901 atggcatacg tgatgccttt gaagctggag taaaagaaac gggtgtcaca attcattata
     6961 tcgaccaagg tgtagatact ggaccaatca tccggcagga aaaagtgaga attgaacagg
     7021 aggacacgtt cgattcttta gaagaaaaaa tccatagagt agagcatcga atctatccag
     7081 aagtgattag tgaaattatt gaaaatggag gctatctgaa atgacaagag cattgatcag
     7141 tgtatcagat aaaactggga ttgtagcatt cgcccaaggt ttgattgaac aaggagtaga
     7201 aatcatttca acaggaggaa caaagcatgc gttggataaa gctgggatca aaacgctttc
     7261 gatcgaagaa gtgacagggt ttcctgaaat gatggatggc cgtgtcaaaa cacttcatcc
     7321 taaaatccat ggtggtcttt taggaagacg cgatctccct agccatatgg aggcaatgga
     7381 aacggaaaat atccaaccaa tcgactttgt ttgtgtcaat ttatatcctt tcaaagaaac
     7441 gattatgaaa ccagatgtcg aaatagcaga agctattgaa aacattgata tcggtggtcc
     7501 tagtatgctc cgtagtgcag caaaaaacca tgaatttgtc acctctgttg ttgatccatc
     7561 tgactatgaa gaagtacttt cagaattacg tcagtatggg catacttcac tttctttcag
     7621 acaaaaacta gcggcaaaag tctttcgtca tactgcggca tatgatgcac tgatcgctca
     7681 atacttaacg gattgggtgg aagaaaaaga accggaaaaa ttgacactta cgtatgaacg
     7741 aaaacaaacg cttcgttacg gtgaaaatag ccatcaagcc gctgcctttt atcagtccgc
     7801 tcttctggaa aaatattcga tcgcagcagc aaaacaactc catgggaaag aactttctta
     7861 taataatatc cgagatgcag atgctgcgct acggatcatg aaggaatatg acgagccaac
     7921 ggtagtcgcc ttgaaacaca tgaatccttg tggtattggg acagcatcta cgattttaga
     7981 agcgtgggaa gcagcgtatg aagcagatcc tgtttcaatc tttggtggga tcatcgtttt
     8041 gaatcgagaa gtagatctgc caacagcaga acagatgcat aagctgtttc tagaaatcat
     8101 cattgcccct tcttatacac aagaagcttt ggaattactg caaaaaaaga aaaacatccg
     8161 attactgaca gtagactttg cgcaggaaga aaatcaaaat cgagaagaaa cggtctcagt
     8221 cttaggtgga cttcttgtac aagatcaaga tctggcaata gaaaatgaag aagagtggaa
     8281 agtcgtgaca aaacgtaagc cgactccagc agaagcaaaa gcattagcgt ttgcttggag
     8341 agctgtcaaa catgtgaaat ctaatgcgat cgtactggca aacgaacgtc agacagtagg
     8401 aatcggagcc gggcagatga atcgagtcgg ttcagtaaaa attgcgattg aacaagcgca
     8461 agagaaaatg gaaggtgcag ttttagctag cgatgcttat tttccaatgg atgattcggt
     8521 tgaatatgca gccaaacatg gtataaaagc aatcattcag ccaggaggaa gcatcaaaga
     8581 ccaagattct attgatatgg cgaataaata cgatctagca atggtcttta ctgatgttcg
     8641 gcattttaga cattagagag gagtcggata aatgaacatt ttggtgatcg gtgcaggtgg
     8701 tcgtgaacat gcaataagta aaaaactttt agccagtccg aaggtggata cggtgtattg
     8761 tgcgccagga aatccgggga tgacaaaaga tggtatcaat tgtgtcgcta tcagtgagcg
     8821 agatcattca gcgttaatcc attttgttaa gaaagaggct attgattgga gtatcgttgg
     8881 tcctgaagtt cctttgttaa atgggatcgt cgatgatttt atggcggaag gattgaaaat
     8941 cttcggtccg acaaaagcag cagctctgat cgaaggttcg aaaagttttg ctaaacaatt
     9001 aatggatact tatcaaatcc cgacagctaa atccagaagt tttgaatgtt acgaaaaagc
     9061 agcagcgtat atcaaagaaa atggtgcgcc aatcgtagtg aaagcggacg gcttagcagc
     9121 tgggaaaggt gtagtcgtgg ctaccacagt gaaggaagcg ctggatgcgg cagaacaaat
     9181 gttgaaccag catcgatttg gggatagtag caaaaaaatc gtcgtagaag agtttttggc
     9241 aggagaagaa ttctcccttt tggcgtttgt ccgtaatacc aaggtgtatc cgatggtcat
     9301 ttcgcaagat cataagcggg catttgataa cgataaaggg ccaaatacag gcggaatggg
     9361 tgcttattca ccagtaccgc aaatttcaaa aaagatgatt gaaaaagctg tggaagaagt
     9421 tttaaaacca gcggctttgg gaatgaaaga actaggacgc tctttcacgg gtgttttata
     9481 tgcgggtttg attgctacga aggaaggacc aaaagtgatc gaattcaatg cgcgtttcgg
     9541 tgatcctgaa acgcaagtcg ttctttctcg gttgaagagt gactttgcgc aagtaatcga
     9601 tgatttatta gagaatcgta cagtagagtt aaagtggcag caagaaggat acaatgtagg
     9661 cgttgtggtt gcagctgccg gttaccccga agaatatgag acaggaatgg ttctaccaga
     9721 ttttctggaa gaagagcttt ctgtttacta tgctggagtt tcctcaaata acggcaaact
     9781 agttggagca ggaggacgtc tttatttagt agaagcatac ggtgagacga tcaaagaagc
     9841 acagaaaaaa atttacaccg cgttaatgga taaaacgaca gatggaacat tttatcgtac
     9901 agatattgga gaaaaagctg ccaatagcga actgatcgtg aagtaaatca gctgcttcca
     9961 tttataaaaa atacaacaaa cataatcgga agcgccgatt ttagtgaagc cttttcataa
    10021 agagtgtggt acacttttgt tgtgtatata aataaggagg agttcagatg aaaaaaattc
    10081 aattaggtac ttctgatcag aaagcagctg cagtgatctt aggctgtatg cgtatcaaca
    10141 atgcggaaga tccagtaaaa gtgattgaga cagcttatga aaacggcatt gatttttttg
    10201 accatgcaga tatttatgga aacggtgaat gtgaagaaat ctttgcagat gctctagcaa
    10261 aaacatcaat caaacgagaa gatcttttta ttcaaacaaa atgtggaatc gttccaggtg
    10321 tgatgtttga tttttctaaa aaacatatca ttgaatctgt agaaaaaagt ttgacccgtc
    10381 tgaaaatgga ttatgtggat gctttacttc ttcatcgtcc agatacattg gtagaaccag
    10441 aggaagtagc tgaagcgttt gatgaattgg aacggtcagg aaaagttcgt tattttggcg
    10501 tcagcaatca aaaaccaatg caaatcgaat tgctgaaaaa atatgttcgt caaccattag
    10561 tagctaatca gctgcaattt ggcttgaaac atactggaat gatcgatcaa gggattcatg
    10621 tcaatatgac agatgaagca agtatcgatc atgacggtag tgtacttgat tattcccgat
    10681 tgcatgatat gacgatccaa gcatggtcac cataccagta tggatttttt gaaggtgtat
    10741 ttatcgggaa cgaaaaattc cctgaattaa accaaacatt agaagaacta gcagctaaat
    10801 atcaaacgac accaactggc ttggcttctg cttggatttt acgccatcca gccaatatgc
    10861 aagtgatcgc cggtacgatg aacgtagggc gaatcaagga aatcgctcaa gcttcagaaa
    10921 ttaatatgtc tcgggaagat tggtacgaat tgtaccgtgc tgctggaaac attttacctt
    10981 aaagattgtc ataagcaggt caaaagggct ataaattaaa aagacagaat aacttgaaag
    11041 ctgttttcag cttcgagata ttctgtcttt ttttattgca aatggaaaga gtatcattgt
    11101 acggatttcg tttattagtt atttttgttt ttaaggaaaa gctgtgtccg atattctttt
    11161 ggagaaatcc cttgtttttt cttaaataaa taactgaaat agttagggtc attataacca
    11221 atttcaaaag caatatctga aatacgatag tctgattgct gcaataattt ttttgctaat
    11281 tcgattcttt gttctgtcag ataatcgatg aatgtttggt taaacgattg agaaaaaatc
    11341 gtactgaaat gagagggact taatgcaact tgttgagcga cccaagttaa ggacatatct
    11401 ggatcggtaa aattttcttt gataaaatcc aatgcatggt tcaaaattgg ctgatatttg
    11461 ttccttgaag gctgtgaggg cagagcagtt aagaaagaaa tgaacatata aactgtcgaa
    11521 cggtaaagcg agtcgtcggc gctgattgct aagcgttgac tggtggatgt gcaatgaacc
    11581 atttcttgcg ggattttctt ttctttcagg cagacatgct ggagtagctt agtcaagaca
    11641 aataaacgat ataatcgtgt tcgagttggt gtgccttgtg gtttcgtcaa gtgttcgatg
    11701 tacttagata cttcgttttt cgataatgaa gacaaatcaa gtgagaggtc aaagtaaaag
    11761 acagaatcaa ctggaagttt cgcttcttga atcaaatcct catagctgat aatttgttct
    11821 ttaagggaac tggcggtttc aagtatcatc tgcgtgatct caaaagatgc aggtaattcg
    11881 ctgattcttt ctgcgacagg accaatggcg acagaaaaat tatttttttg tcccttttct
    11941 aattcatgga ccaacgtttg ggctacctga taactggtct cgatcacatt ttcttcaaga
    12001 gaatcaacta ttaaaaattt gatgaatcga gaggaaacag cagagaagat cacttgttga
    12061 ttctccctaa ataaaaactt caaataatca ctgagatgag cgtaatcttc aaagtctgta
    12121 ctgacttggt ttgctgctaa taatacggtc atttttttcc ctgctaaaga gcgattgaaa
    12181 gattcagcaa gtttcaatgt ttcactgatc gtaagtgagc ctttgaacaa accgttcaag
    12241 aaatgatttt ttttcaattc gaaaacaaga tcagaagaag tgtgcgtttc tttttgctgc
    12301 aaatctaaag cagttactgc tttttctatt gcttgtttca attcgctact tttaatcggt
    12361 ttaagcagat aatcatctgc ctgtacctgg attgctcctt tcatataatc gaaatcgtcg
    12421 aatccactaa taaatatgag acgagtccaa ggaagtagtt tccttgcttc ttttgaaaaa
    12481 gtcaacccgt ccatgaaagg catttggata tcagtcagaa tgatgtcggg ctgtaagtcg
    12541 ataattgaag acagtgccat ctcaccatct ccagcttcgc cgacataagt gattggataa
    12601 taagaaccaa ggacagttat ttgttcccgc aagttattgc gaatgaggta ttcgtcttcg
    12661 atgataaaaa ctttatacat gtttgtttcc tccaattgtt ttgggagata gagggatcat
    12721 cagtgtgaca gttgttcctt ctcggtattt gctgtcaatc gtgataggtt tttgatcatt
    12781 tccgtaataa agaaggagac gcttgtttac gttgtaaagt ccgtagccga tactgaaatc
    12841 tgaatcgatc cctttggcta actctttacg tatttctgcc agtttttctg cagggatgcc
    12901 aatcccgttg tctataactt tgaattggat catctcatca attttgcatg tggtaatacg
    12961 aataaatcct ttgtgacgga cgtacttcgt tccatggtat acagcatttt caatcaatgg
    13021 ctgcaagatc atttttaaaa tagaaacagg aaaaagtgtt tctggtatat cgatcttata
    13081 gtcaagcata tggccgtatc ttatttttaa gatgactaga taatcattga tatggctaat
    13141 ttctttttca agaggaatcc agtcttgtcc tttgcttaaa gacgtacgaa aaaagccgga
    13201 aagtgcatgg gtcatagtca gagcttcttc aaagcagtct tgttcgatca aagaaataat
    13261 ggcatccaaa ctgttataaa taaaatgggg agtaatctga gcttgcaata cacgaacttc
    13321 actttgtgct aaatggtatt gtttcaaagc gttctcccgt aaagactggt ttagatcttc
    13381 cgccatttta ttcatgctag cggtaagtgt ttgtaattcc agtgtttccg gcagggttaa
    13441 acgaaagcca aggttccctg aagaaagttg ttctgacatc tcgatcagct gattgatcgg
    13501 ttcttggacc tttctctcta aaaagcgacg attctttttc gcatagaaaa aaatggtact
    13561 aagaactcca agttccacta ttgttaatag aatgagcgag cggacaagtg tcgtattttt
    13621 tttgcttgct aagtcgatct ccacacgaac aaaatcttgt aagatatctg acagcagttg
    13681 agtgacagaa tctacctgat tgatgatctc ttcattggta tccataaaat cttttgccaa
    13741 gatattattg atcagctcgt tttgataatc ttccagtgta tcgatcgtac gtagcgatac
    13801 atctaacgta ctaatttctt taagtgtatt tgtattattt ttgatctctc gaatatcatt
    13861 cctaagttct tctaggatat tttcctcatt gaactcatct acggttactt ggccagaaat
    13921 caagtcccac atactttcca aaactttttg atcaaccttc aaagcaattg catctgcttt
    13981 ttggatattt gcgatcgtct gttgataaat caaaagctgt tgcgaatata acagtatgct
    14041 cagtagcaaa gggataacag caaatagaag catggctttg tttgcttggg caaaaatctg
    14101 ttttatgctt aaattttttg tcatgatact tctccagtca ataatttctg gttgggatgt
    14161 tagctatatt ctgctgggaa aagacagtct cggaaataaa aatatcttta ggtaatgttt
    14221 ttcctgtttg aatatttcca aaataacgag taacggtatt ggctacatac cagccgacat
    14281 caggtttaca ctcgactaca caattgacga ttcctgagcg tagcttctcg atcatatctt
    14341 cttgtccatc aatagttaca atgatcaaat cttctccagg tttgattgat gtttctttta
    14401 tcgcagctaa agcaccgtga gtcatttcat cactatggct gtataataca tcgatttcgg
    14461 ataaatcatt tgtttctaaa tatcgtttca tctggtcttt tcctttcatc cgaataaaat
    14521 caccgacaat tcggtgaaca atagtcatcg gaggtttatg gtcgatggtt ccatcttttt
    14581 ttagggttgg tttacgattg attgattcca aaaatccttc ggagcgtagc ttggtcggtg
    14641 tggagttctc tgaaccagca agttctagga ttctgatttc ctgtttcttt gaatttgtga
    14701 agtgattgct gacaaataaa cccgcccgat ttccttctgc tttgaaactg gggccaatgt
    14761 gggtgacgaa aagctcggga tcattcgttt gaatatgtct gtcgacaagt atgacaggga
    14821 tccctgctcg ctttgcttct tttaatacag agtcccaccc ggattcttgc aaaggagtaa
    14881 aaatgatcat atccacttta taggcaatga acgtccgtat atcttggatt tgccgttcct
    14941 gattcatgta gccatttcgg tagagaactt ggtaatcttc tttttcaagc gcagtgataa
    15001 ttgattcagt atgctttttc cgccagctac tctccgttcc tgattgcgaa aaaccaacga
    15061 ctagtgggtc aggtgcttcc tttgtattag gtgtacaccc catcatttgg taagcgatta
    15121 caacaagggc taaaaaaaat aaaacacgtt tcactttaaa tcactccatc tggatttgtt
    15181 agtactatct tatcagataa aagggaaaat caaagtcaaa aggaatcaat cgaaaaaaat
    15241 ccaagaaact taaaaaaatc caagacaaaa aaagtacacc taaaaaattt aaagtaaatc
    15301 ccataaaaaa atcaatgtta acgctttctt tattagctat actcggtgta tcaattgaaa
    15361 tgggggataa aaaatgaaaa acaaatttag aaagattgtt gggactatcg ctattttgac
    15421 cgcaagcatt ctattaagtg cgtgtggcaa ttcaggatct gcagatgata gtacggggta
    15481 tgtaggaatc gctatgccga ccaaatcagc agaaagatgg atcgctgatg ggaacaacat
    15541 ggtctcggaa ttagaaaaac tgggttataa aacagatctt caatatggag aagataaagt
    15601 agaaaatcaa gtagcacaaa tagaaaatat gattacaaaa ggggtagata cacttgtgat
    15661 tgcttctatc gatggttcgg ctttaactga tgtgttagct aaagcaaaag aagcagatat
    15721 caaggtgatc gcctatgatc gtttactgat gaattcagaa aatgtcgatt attacgcgac
    15781 ttttgataat tttggagtag gtgtatcgca agcagcttat attgaagagc acttaggatt
    15841 gaaagaagga aaaggaccat tcacgattga attatttggt ggctcgcctg acgacaacaa
    15901 cgcactaatc aattacaacg gagtgatgtc tgttttacaa ccatatatgg acaacggtca
    15961 gttggtcgtg ccatctggac agacgagttt cagtcaaatc gctacgcttc gctgggatgg
    16021 ttctacggcg caagcaagga tggataatct gttaagcgca aattatactg accaaacatt
    16081 ggatgctgtt ttgtcgccat atgatccaat aagtttagga atcatttcgt cattaaaagg
    16141 cgtggggtat gggagtgaaa gtaaaccttt acctgtgatt actggacaag atgcgacagt
    16201 tgctggcgtt aaatcgatca ttgccgggga acaaacacaa acgatcttta aagacacacg
    16261 tatcttagca aaaaatacga ttgaaatgat caaagcaatc agtgatggag aagaggttcc
    16321 ggtcaatgat actgaaactt atgataatgg ggtgaaaact gtccctacgt atttagcaaa
    16381 tacagtatct gtcgataaag ataattatca agcagagctg atcgacaccg attattataa
    16441 agaatccgat ctgaaaaatt aagaggaacc aaacaaggaa ctaaagagat ctaagtcaga
    16501 gagaggataa gaattatggc ggattgtatt ttaaaaatgg cagatatcac caaaaaattt
    16561 tctggaatcc gagcgttaga ttgcgtgaat ctgatgattg aacgtgggga aatccatgca
    16621 ttgtgcggag aaaacggagc gggaaaatca acattgatga atgtcttgag cggcttgtat
    16681 ccatatggct cctatgacgg ggaaatcttt tatgatggaa agctgtgtca atttaaagat
    16741 ttgaaagata gtgaagaaaa aggcatcgtc atcattcatc aagaattagc actaagtcct
    16801 tatctttcaa taaaagagaa tatttttctc ggaaatgaac aagcagaaag aggcgtgatt
    16861 gattgggaac tgacagaaaa gaaaacatat gaactgctga ataaagtcgg tttgtctatc
    16921 gacccgaatc ttctagtatc ccagattggc gtagggcaac agcaactagt tgaaatagct
    16981 aaagcattat caaaatccgt ccgtctgttg attttggatg agcctaccgc tgcattgaat
    17041 gaagaagaaa gcgcgaattt gctgagattg atccgagagt tgaaagagca aggtgtaacc
    17101 tccatcatta tttcacataa gctgaatgaa atcgtggata tcgcagatcg aatcaccatt
    17161 ttacgggatg gacagacgat tgagacacta gaaaatgaaa tgatcagcga agagcatatt
    17221 attcgcggaa tggtgggcag agatttagtt gatcgttatc ctgaaagaaa accggatatt
    17281 ggagaagttt attttaaagt agaagactgg acggtctatc accctcttga tcacgagcga
    17341 atcataaacg accacatcaa cttgcatttg aaaaaaggag agattgtagg aatagctgga
    17401 ctgatgggag cgggcagaac tgaattcgct atgagtctat ttggacattc ttatggaagc
    17461 aagatatccg gacgtatatt aaaggaaggg gcagagatat cagttaagac cgttccttca
    17521 gccattgcaa acggactcgc ttatgtctcg gaggaccgaa aatctttagg actgaatctg
    17581 ctgatggata ttcgggaaaa cgtgacgctg agcagtttgg ataaaataag tcaaaaagga
    17641 gtattagata aagaaaaaga agtaactgag gcagaaaatt tccggaaaaa gatgcgtata
    17701 aaaacaaact ccatccatca aattgtcagt agtcttagtg gtgggaatca acaaaaagtc
    17761 gttttatcca agtggctgat gacacaaccc gatattcttt ttttggatga gccaacccgt
    17821 gggatagatg ttggggcaaa atatgagatc tacacgatta tcgaagaaat ggcagcaaat
    17881 ggcaaagctg tctgtcttat ctcttctgaa cttcctgaaa tattagggat gtgtgatcga
    17941 atctatacga tgaacgaagg acgtattaca ggagaagtct cgcgagaaga tgcagatcag
    18001 gaagtcttga tgaaattaat gacgaaagaa gaggtgggtt aagttggaac aggcaaaaaa
    18061 tcttacggat aaaaaagaga aaaaactaaa tttgaaaagt agagcagctg atctttttgg
    18121 tcgctacagc atggtgatca ttttagcggg actacttatt gcgtttcaat tgatgacgga
    18181 tggtattttc tggcgtccat tgaatataac taaccttgtt ctgcaaaata gccatatatt
    18241 agtactagct gcagggatgt tattagttgt gctcttaggt catgtggatt tgtcggtagg
    18301 gtcagtgatg gcgtttgttg gtgcaattgc gggcatgatg atggtcaatc gccaaatgaa
    18361 cccgtggatg acagttatcg catgtcttgc agtaggcggg ctgatcggcg catggcaagg
    18421 tttttgggta gcttatgcag ggataccggc gtttatcgtg actttagcag gattgctgat
    18481 gtttcgtgga ctgacacaag ttgttctagg aggccaatct ctagcacctt ttccagctgt
    18541 tttccaaaaa atgtctacgg gttatttacc agacttaagc gaaggaagca gccttcactt
    18601 cttgacattg attttaggaa tgatcatcac gatcgtgtta gttgttggtc aatggcgaac
    18661 aagaaatcgt cggaaaaatc atttatttga agtggaatct ccaacagctt tcttggtaaa
    18721 ggcggtattt acagcagtcg ttgtactagg aataacttat atctttgcct cttatcaagg
    18781 gtatccgatt attttgatca ttcttggttt gatcgttgca gcatatgggt ttttgacaaa
    18841 taaaacagta gcaggtcgac agatttatgc gacgggcgga aatcgaaaag cggctgaact
    18901 atcagggatc aaaacaaagc gaatcacgtt ctgggtattc gtcaacatgg gagtcatggc
    18961 agctttagct ggattagttt tagctgctcg attgaatgcg gcaacacctc aagcaggtac
    19021 atcgatggaa ctggatgcaa tggcggcagt atattttggt ggtgcttcga cttccggcgg
    19081 cattggaaca atcatgggtg cgattgtcgg tggcttggtc atgggtgtat tgaacaatgg
    19141 catgtcgatt ttaggcgtcg gggtagactg gcagcaggca atcaaaggat tgatcttgct
    19201 tttagccgtt gtgctagact tgtataataa gaaaaaacga acaacgtagg agaacgccgg
    19261 ttattcagaa aaaagactgt gacaagacat ttgtcacagt ctttttttcg ctaaaagcga
    19321 acattcattg tttcgtttgt atagactaaa aataatatat gtttttttac cattcgtata
    19381 aattctcttt ttgctcttga ttt
//
LOCUS       DAZAID010000055        18849 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000055 DAZAID010000000
VERSION     DAZAID010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.055"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..1570
                     /locus_tag="ACG9BE_001783"
     CDS             143..1570
                     /locus_tag="ACG9BE_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3032525.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1662..2027)
                     /locus_tag="ACG9BE_001784"
     CDS             complement(1662..2027)
                     /locus_tag="ACG9BE_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFN3032526.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2024..2389)
                     /locus_tag="ACG9BE_001785"
     CDS             complement(2024..2389)
                     /locus_tag="ACG9BE_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFN3032527.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2581..2829)
                     /locus_tag="ACG9BE_001786"
     CDS             complement(2581..2829)
                     /locus_tag="ACG9BE_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032528.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2828..2956
                     /locus_tag="ACG9BE_001787"
     CDS             2828..2956
                     /locus_tag="ACG9BE_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFN3032529.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3013..4707
                     /locus_tag="ACG9BE_001788"
     CDS             3013..4707
                     /locus_tag="ACG9BE_001788"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFN3032530.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4807..4980
                     /locus_tag="ACG9BE_001789"
     CDS             4807..4980
                     /locus_tag="ACG9BE_001789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032531.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5358..6491
                     /locus_tag="ACG9BE_001790"
     CDS             5358..6491
                     /locus_tag="ACG9BE_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFN3032532.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6522..6842
                     /locus_tag="ACG9BE_001791"
     CDS             6522..6842
                     /locus_tag="ACG9BE_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFN3032533.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6871..7335)
                     /locus_tag="ACG9BE_001792"
     CDS             complement(6871..7335)
                     /locus_tag="ACG9BE_001792"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3032534.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7467..8720)
                     /locus_tag="ACG9BE_001793"
     CDS             complement(7467..8720)
                     /locus_tag="ACG9BE_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN3032535.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8749..9066)
                     /locus_tag="ACG9BE_001794"
     CDS             complement(8749..9066)
                     /locus_tag="ACG9BE_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN3032536.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9092..9412)
                     /locus_tag="ACG9BE_001795"
     CDS             complement(9092..9412)
                     /locus_tag="ACG9BE_001795"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3032537.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9876..11291)
                     /locus_tag="ACG9BE_001796"
     CDS             complement(9876..11291)
                     /locus_tag="ACG9BE_001796"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3032538.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11308..12624)
                     /locus_tag="ACG9BE_001797"
     CDS             complement(11308..12624)
                     /locus_tag="ACG9BE_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3032539.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12774..13472)
                     /locus_tag="ACG9BE_001798"
     CDS             complement(12774..13472)
                     /locus_tag="ACG9BE_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFN3032540.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13761..14750)
                     /locus_tag="ACG9BE_001799"
     CDS             complement(13761..14750)
                     /locus_tag="ACG9BE_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN3032541.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14830..15663)
                     /gene="msrA"
                     /locus_tag="ACG9BE_001800"
     CDS             complement(14830..15663)
                     /gene="msrA"
                     /locus_tag="ACG9BE_001800"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN3032542.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15838..17472)
                     /locus_tag="ACG9BE_001801"
     CDS             complement(15838..17472)
                     /locus_tag="ACG9BE_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFN3032543.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17459..18844)
                     /locus_tag="ACG9BE_001802"
     CDS             complement(17459..18844)
                     /locus_tag="ACG9BE_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032544.1"
                     /translation="MCTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAI
                     AEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIV
                     RLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEA
                     LSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTN
                     LFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFL
                     LVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKH
                     EVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKH
                     GLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVT
                     KKEINKKNENK"
BASE COUNT         5964 a         3713 c         3145 g         6027 t
ORIGIN      
        1 tgttttccaa attttataac ataaaaaacc ttttttaacg gcatttatat gctttttcag
       61 aaattaactg atgatttcaa aaagaaaaca tgtcataata tttcccatat tgtgcaaaaa
      121 aatacaaggc ggaggctcta ctatgattga agaatatatt gagaaaaata ttttaagaca
      181 gctgttttta tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa agctctccaa
      241 tctcctgcat gtttgcaaaa ccactttact caatgatatc aataacataa aaaaagaatt
      301 tgaagaacag atcgcttata cacatcggga aaaggaccgt tacactttat atttttcaga
      361 acatatacct cgatgcaaga tcatgcagca attgtctcaa aattcgttgt ttttaaaaac
      421 ttgtctgctt tatttggaag aagatgaacc tgattatctt caattaactg aatgtgaatt
      481 catctctgtc agcaaagctt attcattaaa aaaacaagta ttagcttatt ttaatgactg
      541 cgggatcgaa atcgaccgct actcccctcg attcactgaa atggaaagac gtctgctgct
      601 tcttaatgtg tcgtatcgtt taggcggttt taattcttgg gagctacctg aatcgttttt
      661 tgaacgtgcc gatcgtttca ttgaatccgt aacggaaaat agtggcagat tttatgataa
      721 agaaaataaa gaaatcttat cgattggttt tgctatcagt ttcctacgcc agcaagtgtg
      781 tgctgtgaca atagattcaa aatttatcga agaaatcaaa aaacgtccta tttacaatta
      841 tgtcgaatca gcatgggaaa acactgattt ccaaacctat tataaaaaag aagaattcgc
      901 ctttattttg acactattca atctttgtaa ttatggtttc cattcttatc agttgattgc
      961 ggaagacttt caacagctac accaagtgtt tattgacaat actcccgaaa taaaagaact
     1021 agttgctacc tttgaatctc acttcaatca agaattattc ggaaaccaac cgttcgaacg
     1081 tgcgctgatt catctgatgc gttcggcttg ggataactat caattattca tgccagaaaa
     1141 attttatctt ttaaatgagg aacagacaaa tttatataaa gaagtccaga caattttttc
     1201 cagctggtcc agccagttac cttatgatct acggttgaat cctaattgca tgcgggcttt
     1261 tgtgatcgaa ttgagcggaa ttctccgtct gacaaaagaa catctgacta tctatatcgt
     1321 cacaaattcg gatgttcatt atctgattta tcgggaagcc ttggaagctg taactacttt
     1381 cgattttcaa gttgcaccaa caatttattc aagtatatct gatataaaga agtatgcgca
     1441 gcagtcctca aaccgggtat tatgtgaacg tacattatat acaccagatg cggtccaata
     1501 tgaaaatatt attcctattt cgatcaatac gatcgatcga gctatcattt cagctgtgca
     1561 aaacaaataa ggggctgtga caaaaatcat gtcacagcct cttatttgtt gaagatgccc
     1621 atttctgtaa caatctcttg atacacgtca ggctcacaac ctcacttatt ttggacatct
     1681 acgggtccga tctctttatt taaaaaataa gaaatgatgg ttcggataac taccaacgcc
     1741 gctagttgga aaatatcctg caaggtaggt ttgataatcg attcgatgat cccagctgaa
     1801 acaagaattt ccaaacttag aagtatatag cctcccaaca tctttttaat agcattgttc
     1861 tcgctgactc ggtcgctttg cacttttgta tggacaaata aatatttcaa aaaatccttg
     1921 attgatagac aaacacccca aaccaataaa cagatagcaa agatatcaag tcccagtaca
     1981 agtttttcga aatatggaag aagaaactgc atcaactctg tcgtcatcct acctctcctc
     2041 ttctttgttt ttctttgtct tctatttcct gattcaagaa ataagaaatg actgttcgaa
     2101 tgataataat aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata actgactcga
     2161 tgatatcagc aacgactagt acttccaagc ttaacaaaat ataacttgct agcagttttc
     2221 ttgtatctgc attacgagta ttacgttctg catagttttt cttgacatga gaccaatcaa
     2281 aaagtttgaa tagaacaaga acgatcccga caattaagat accgatagaa caaatattta
     2341 aaatagcaat gatccattct agaaattcaa ttaaatttga gcgaaacata tacgctacct
     2401 actttctttt aatttgttcc tattctttta ttgtaaaaga aagttacctt gttcgtctat
     2461 ttaaaagttg ttctaatgat aacggttcgc ttaaattttt ctttttcact ctcccatttt
     2521 tctccctcca tccataaatc gtggaagtat ccaaaacaac gtagcaagtt aaaataaggc
     2581 ttagtccgtc atcagctttt caaagaagca ctgtctcggt gctttttttg tacccaaatt
     2641 tctaaaaata gaaccataca cctcctcaaa tcttttcttc ttacctaccc tatttttatt
     2701 tacaggaaaa tcagacactt cttctttttt gtcaaaattt cctttttctt ctcgtcctat
     2761 catttggcat tctaataaaa acgaactatc cttggatata gaaagagaaa acaaggaggc
     2821 gtttttcatg agtaaaaaat taatcaaagt cggcatcggt ttaggtttgc ttgcccttgg
     2881 cgctgcatat ctaggaaaaa agacaggact attcgaagat gacagtcatt tatatgatga
     2941 attcgaatca atctagcatc ctatttagtt gaatcactac ggacatactt ggaagacatt
     3001 ggaggaatca caatgagaaa aagtaaagca ataatgattg gtgccggtat tgccaatatg
     3061 gcagcggctg tttatttgat tcaagaaggc aaatggaaag gggatcagat caccttttat
     3121 tcattggatg atcacggttc aaatgatggt gcaccgaccg acactgtagt ggatgaatat
     3181 tggaataaaa accatccatt agaaaaccaa aaaggctata ttgctcgagg cggtcgcatg
     3241 cttaattacc gtacctatgt agatttgatg gatctgttaa gtcgaatacc ttctgttact
     3301 gagccaggga tgactgctga agaagatacg agagattttg atgcaaaaca ccgaaccttt
     3361 gacaaagcac gtttgatgga aggtggcaaa ggaatcattg atggcggaaa actgggattc
     3421 aataataaag accgtattct tctgactaaa ctgattgcta tgcctgattc agaagaagaa
     3481 aaactagaca atatcactat cgaagaatat ttcaaagatg atccacactt ttttgaaaca
     3541 aacttctggt ttatgtggga aacaacattt gctttcagaa cacgtagttc tgctcaagaa
     3601 ttgcgtcgct atatgcatca aatgatttat gaattcactc aaatcgaaca tctagtgggc
     3661 gtcaatcgaa cacgttacaa tcaatacgaa agtattatgc ttcctttgat cgagtacttg
     3721 aaagaacaag gatgcaaaat cattttgaac cgccgtgtca ctgactggga attcaaagaa
     3781 acaccgatgc aagatgaaat cactgtgact ggtttgaaaa ttaccaatac actgactcaa
     3841 gaagaagaat acgttgtggt cgatgaagat acagcagtta ttttcactaa tgggtccatt
     3901 acagattcag ctactttagg agattacgaa acaccagctg tagaaaatat ggattatggt
     3961 gcagcttcta gtctctggaa aaaagccagt gaacgtttct acaacttggg aaatcctgac
     4021 aaattctttg ctgatcgaga tgcaagcgaa tgggtcagct ttaccctgac gacgaaaaac
     4081 catttgttgt tgaatgaaat cacccgtatc acgactcaag aaccaggaaa tgccttgaat
     4141 tcatttattt caaccacacc gatcacgccg ttaggccaaa aagatgtcaa tatgtctatt
     4201 gtcgtgcatc atcaaccgca ttttacgtct cagaaaccaa atgaaacagt tatttggggt
     4261 tatttccttt atccaagacg acgtggcgaa tttgtggata aacagtatat taaaatgaat
     4321 ggaaaagaaa tgctagaaga actgattgga caactttcaa aagtagatcc tggaccagtg
     4381 aatattcgtg aaaaagaaac agaaattttc gacagcgtaa taaataatat ccctgtctat
     4441 atgccttatg cttccgcgtt gttcaataat cgtgccaaaa gcgatcgacc aaaagtcatt
     4501 cctaaacatt cgaccaatct ggcctttaca ggagaatttg tcgagcagcc ttatcagatg
     4561 atctttactg aacaaagtgc tgtacgttct ggagaaatcg ctgctttcca ttttgcgggt
     4621 attcctatgt cccgtcttgt caaaacaccg cgttacgaca aagatattcc tactctagct
     4681 cgtgccgcaa aaaaaatgtt tgaataatca agaggttgtg aaaaagctgc cctgcatcaa
     4741 gtaataagaa caaccaggct aaaacagctt cttatgtttt tgatttggtt ggacttatta
     4801 ccgcagatgc aagcttttga acaccgttta ccaagaggtt atgaaaaagc tgccctgcat
     4861 caagtaataa gagcagccaa gctaaaacag cttcttatgt ttttgacttg gttagactta
     4921 ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa aggcgtttag
     4981 tttcgagtat gaagaaaaat tttgtgaaaa tggttcctca cgtttttgca taaatttgac
     5041 ttaataccga gaaactgatc ccttctgtca taacctcttt ttatttatgt ctgtttcttt
     5101 ttgaaaacaa ctattttttc aacgtttact ctcttgccaa tccaacatca tctaacggat
     5161 acaactattt caatgattac cattacacta aaaaatcgga aacacacctc tccgaacaca
     5221 aatacataag actatagaat gtttcattta agaaaaaaag cgtttcatta tcaaaaaggt
     5281 aagatctcct ttaaaatgaa attatctgta gtactaataa tgcagacaat tttatgaatc
     5341 tgaaaggagc atctccaatg aaagcagtca cttggcaagg aaaacagaaa atggaaatca
     5401 gaaacgtaga tgatccaaaa atattagaac caacagatgc aatcatccgt atcacagcaa
     5461 cagccatttg cggctccgat ctgcatcttt accatcatgg tgaagcagtt ttggaaaaag
     5521 ggtatgttgt aggacacgaa ccaatgggaa tcgttgaaga agtgggatct caagttcaaa
     5581 cattgaaaaa agggcaacgt gtggttatcc cttttaatat tagttgtggt cattgtcatt
     5641 tctgcctgaa tcacatggaa agccaatgtg atgaatcaaa tccagatcag ttatatggcg
     5701 ggcttttcgg atttggcaag ttgaacggta atcattgggg tggtcaagcg gaatatctaa
     5761 gagttcctta tgcagactct acttcatttg ttgtaccaga taatgatctt cctgatgaaa
     5821 aagttttatt tctatcagat attttgccca cagcttattg gagcgttgaa aacgcaggag
     5881 taaaaaaagg cgatacagtt gtgattctag gttctggtcc agtcggtcta ttcgctcaaa
     5941 aattcgctgt tatggctgga gcagagcgcg taatcgcagt agatccagta cagcaccgtc
     6001 tagataaagc ggctaattac aatggggttg aaacttattt cttagaagac acggcacaag
     6061 ctggaacaga attgtatgaa ctggctaaag gtggcgcaga tgtcattatc gattgtgtag
     6121 gaatggacgg attagagcct gtcaaagaaa aagcgaaaaa tttagtaagc ttacaaagcg
     6181 gcacaatctc tccattacag atggcatcgc aagctgtcaa aaaatttgga acagtccaag
     6241 taactggtgt ttatatgact ccagcttctt cttacccgct gcaagaattt tttatgcggg
     6301 atatcgctgt caagcatgga caggcaccaa ttatccatct aatgccaaaa atttacgcaa
     6361 tgattgcaga aggactattc gatcctacac aaatcattac tcattcgctg ccactatcag
     6421 aagcagcaca agcatatgag atctttgaca aaaaagaaga taaaaatata aaagttgttt
     6481 tgaaaccttg aattcagtaa aaaatacagg tgattttctt tatgcccaaa atcaaacgaa
     6541 atacccatga gatcgaaata ggaaaagaag aagatatcga aatacgagga cgtcgcttcc
     6601 gcttctattt ccaaaatcgt tacacattga tctcactggc tgtcgattta ttaaccggga
     6661 ttttctatat ttttggaagc cttgcttcct taactaaaat ccccgatcga tatggcacct
     6721 atttttattt ggctggtgct attttcttga cgattcgtcc tattctcaga atcgtaagga
     6781 acatttttat ttacgacgaa aaaaaagcaa tgaagagaac aagcaaaaaa aaagaggcat
     6841 gagaattttt caaatctcat gcctcttttt tcaccctatc gtatcacttt gatacagacg
     6901 agcggtcgtc ccactttctt ccttgaccat tgtcaaaaat gtccactcgt atcgttcata
     6961 aaacgtggta tgatctgtaa ttaagtaaag tttttcgtac ccaagttgat gcatttcttt
     7021 tctgatttcc gctaatatcc gcttggctat cccctgctta cgcttttccg gttcaacaaa
     7081 cagggcacaa agatttggtg tcagatcagg gcggttatgg aagtcgtttt caatgacgcc
     7141 cgctcctccg acgatggttt ctttttcatc taagaccaca taccattgag gaatactttc
     7201 cttattagcg attccagtac gaatactttc ctcatacaca acagcaggaa ctccccattt
     7261 tgatgaaaac caattcgcgc tttgttttac taattctggg tgttctctta atttgatcca
     7321 gttatatgta accaatgttt aaacctccga ctaaaataaa acgtttacaa aaattataca
     7381 agtctttatg aaaattagca atgacaacaa tagtgatgta aaaaaaaaaa cggaaaacaa
     7441 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
     7501 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
     7561 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
     7621 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
     7681 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
     7741 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
     7801 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
     7861 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
     7921 ataaacggtg cgctatgtcc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
     7981 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
     8041 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
     8101 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
     8161 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
     8221 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
     8281 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
     8341 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
     8401 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gaaagcaatc
     8461 aagacagaaa gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc tttccatgat
     8521 tcaccaccaa aaattgagtt catcaattct tggaacccgg gaatcggtaa gttattgatc
     8581 attactgcta atgctcctaa gatcattaac ggcatactca ccataaatga gtcccgaatc
     8641 gcaactaaat gccgttgtgc accaattttt gatgctacag gtataaaatg tttctccata
     8701 aatgcaacaa atttatccat aaatcttccc tcttttcttt actgtagatt acgcgttcaa
     8761 tcgacgatac agatcaatga tctcttttgc taaatctgta aaagctatag atgtcatcag
     8821 atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt gtgcttcttg
     8881 tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa gattcctctg catctttgat
     8941 tttttcatct gctaaagtga aatcaccttt tttcgcagca gcaatcgctt ccattgcatc
     9001 gctttttgca tttccgccaa acatgatcag acccatgatg gcttctagat ttttttgatc
     9061 ttccatttat tgtatcctcc gataattttt attatttgtt tattagtttt tctgcttggt
     9121 cgagtacttt ttcaccattc atcatgccat agtcagccat attgattaca tctaaaggaa
     9181 tgcctttagg tgctagcttt tcagcaaatt gattttgcat aaagcgtact tgcggtccta
     9241 aaagtaaaac gtctacattt ttagattcta gttgattatc cgcatctgaa gcagatacag
     9301 caaagatgtc tgcttccatt ccttttgctt ctgctgcctt ttgcattttt gtgactagga
     9361 gacttgtact cattccagct gaacatacaa gcatgattgt tttcttttcc attttaaaca
     9421 cgttcctttc tatacttaca cttgtatata agcaagtagc gtgccaactt taaaagcgct
     9481 ttaaaaagtt gatatcatca catttaaggt ctataccaag ttgcatacac actaaaaaag
     9541 aaagcgcaca ctaaacacac aaattaattt ttattacctt ttttctttta acgtgcacaa
     9601 aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa taaacggtat tcaaaagctt
     9661 gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt tactttgcta
     9721 ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg tgttcaatca
     9781 gctgaccctg ggtattagct caaaacgctg gattcacaac ctcttttatt tcaaataacc
     9841 agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc gtcaggcaaa
     9901 tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt caatgttctg
     9961 cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa tgcattcatt
    10021 ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc gccttcttct
    10081 gttgctttga tcaagtaatg caaatgctgt ccgataaatt cgatacggta atcatcttgc
    10141 acgattcctt tctcatccat atactgttct tcatattctc ttcccatacc gttttctgtt
    10201 atcagccaag ggatattatg ataattttct tttagataca tggctatatc atacatgatt
    10261 ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa atcaaagact
    10321 tcataatatc gttcaggatg gaaaggtgtc tcttcattcc attgataacg aggggcgcga
    10381 acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg ctgaatcgtt
    10441 tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa ctccaggaac
    10501 tcttctgtgt agtatccttt taccatcgga tcaaaaaata ctcgattata aaacaaatca
    10561 tacatataag cagctttttg gtcttctgga gaattggatc gagcatagac catttcagga
    10621 tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc tactgcttta
    10681 gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt ttgttcatgc
    10741 ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc gttgaatgta
    10801 aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata cttagcataa
    10861 aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa agctgggact
    10921 tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga gtcgatcatt
    10981 tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc agtaaaaaaa
    11041 cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat cagatccaca
    11101 tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc attatgccaa
    11161 acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc tttttttcct
    11221 ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt ttttggaata
    11281 gtaattttca tgatttcctc ctgtttttta gatcccatca ttttccacac gatttgctac
    11341 ttttacaaat ggcagataga tcaaaataga aacaataata caaacaagtt gcgtaataac
    11401 tgcaccgata tttcctgctg ttgccagata agcattgacg atcggcggca ttggccaagg
    11461 gaccattaca actgctttcg ccgcaaatcc taaagcggtt gcaaaataac caatcgttcc
    11521 agtgataaga ggcgttaaga taaacggaat agctaaaatt ggattcagca tgatcggcat
    11581 accaaaaata actggttcat tgatgttgaa gatcccagga gcaagggata acttggtgat
    11641 ttgtttcata tcttcccgtt ttccaactaa caatactgca atcagtaaac cgatcgtgac
    11701 tccagacccg cccatactca tatacatatc ccaaaatggc atagtaataa tgttcggtac
    11761 ttcttttcca gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa gcaacggttc
    11821 ccgtatcggc ttcaccattt gatttccatg gataccaatc acccaaaaga cttgcgcaat
    11881 aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg ggcgttgtac
    11941 gacattgtaa atgatatcat aaaggtacat tcctgtaagt tctttgatga taaatcccgc
    12001 agccgcgata cccgatacag tcagaatcgt cgggaataaa gcagaaaaac tagcagaaac
    12061 attcgttggc acggtatcag gcattctcag ctgtagtttc ttctgtttcc tcaaccagca
    12121 ataaagttca atagaaagga tggagaccaa cattcccaaa aataaccctt tcgtatctgt
    12181 atattgttta gccaatacgt tttctactat aacagtctga ttttcattca ctaattcata
    12241 aacagttgga ttaacagata aataagacat cactgcgatg ataccaggga atacaccatc
    12301 cactttattc agcttggcta attcgatccc tatcagaaaa acagcataga tggtcaagaa
    12361 acttagtgtg acatagctga tggcagatgc gataggcttt aattcttcta aaaaacgcaa
    12421 gaaacttatc tgtgctaagc cattttccga gtcaaagacc attgctgaaa acaatgtgcc
    12481 aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat gcgtttttta tcaccatgat
    12541 ataccttaaa ttattgattc tcgttgccac gttccctaaa acattggcta atttatccat
    12601 aaaactcatt ccagatcccc tcattcctgc ttcttctcct tgacgtcatc tctttttgtc
    12661 tattagtcag cgcttacatt tcgatgaaca gaaactattt actcctcttt tttaccaaat
    12721 ataaaacaaa aattcatctg atgtatttat cataaaatac atcagatgaa ctgtcaatta
    12781 gtttcttttt ctcctattat tttctgtaat tctctccgat ttattgccat atggacagtc
    12841 attgcatcaa acgcagcaag tgcattccgc ctttcgatgg cctgaacgat ctctgtatga
    12901 atatcgatcg tctcgatttt cagatgctgg atatcataat ttttattaaa caaagcaatt
    12961 gattcattaa tgattgggat aatatgcagc aaggctacgt ttccgctagc ttctgcaatc
    13021 atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg tgccttctcg aaaagaagtt
    13081 tcaatctttt gcgttaattt tttcatttgg acgatttgat tcttcgttgc atattccgct
    13141 gccaaacctg ccatcttagg ctccaataaa tatctcagtt caaataagtc tcgtatcatc
    13201 cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc tcgcttatca
    13261 ctgacaaatg tccccgctcc ttgccgaact tcaaggatat tcctggaagc tagagcccgc
    13321 acagcttctc gcaccgtgct tcgccccacc tctaaataat cggacaattc atattcattc
    13381 ggcagtttat ctcctacttg caactgcatt tcctttaaat atctaaggat cttgtcagta
    13441 gttacttcta ctagggtttt attttcttcc aaaccacata cctccttttc atctgatgta
    13501 ttttttcgta attattataa cacgtttgaa tcatctgatg attttttgta tacaaaattg
    13561 attaatccgt tcatagacat acaaacgaag aagatattta ccataaaaaa gactaggata
    13621 aaattcactc ctagccttcg atcattttta ttttgaacga caaaaaatat gatgttcaat
    13681 aacagacgat cttcattcga ttcttgatgt catcttttgt tttttttaat atatggataa
    13741 aaagtttgat tacatcgttt ctattctaga atttggtcga ttctcaattc ttcttctggt
    13801 gagaaatcta aattatccat cattcgaaca ctttcttgta actgacttaa ccgacttgct
    13861 ccgaccagta cacttgtaac agatttttgt cgcaaattcc atgcaagggc catttgcgct
    13921 aatgattgtc ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc cagagttgat
    13981 ccaacttgtt cttctgaaag gaatggtatt tccttacgat gtgctctaga atcttcagga
    14041 ataccatgta gataacgatt tgtcagcaac ccttgagcaa gcggactgaa cgtgattgcc
    14101 cctagccctt cttcttctaa tactggagat agatcgtctt ctagtagtgc acgagaaaac
    14161 atattgtagc gcatctgatg aataatgaac ggtgcttcct ttctttttaa aatttcagtc
    14221 attttttttg tatcttctcc attatagttg gatataccaa tatataatgc ttttccttgt
    14281 cttacaagtt gcatcaaagc ttctgctgtt tcttccaaag gtgtatcagg gtctgggcga
    14341 tgatgataga agatgtcaac ataatctagc cccattcttt ggagactttg gtcacaactt
    14401 gaaatgatgg atttacgaga gccccattcc ccatatggcc cttcccacat atgataacct
    14461 gctttgctag aaataatcag ttcatcacgg taggcttgaa aatcttctct aaaaatacgc
    14521 ccaaagtttt tctctgcagc tccagcaggg cctccataat tattcgcaag gtcgaaatgg
    14581 gtaattccca tgtcgaatgc gcctcttaaa atctctcttt gattatggat aggatcataa
    14641 tcaccaaaat tttgccataa tccgagagat aaaatcggta atttcaaacc tgaatgaccg
    14701 gatttacgat attccatttt ttcataacgt gcactatctg caatatacaa tttttctcct
    14761 cctcatcaaa tctttttaaa acgctttctt ttttctattt ttagtttact acattttaaa
    14821 gaaaaggagt taatcaaaat gattttttcg tatatttttt tgccatttgt tttttactcg
    14881 ttgatagatc agaaattgat tacgtgcttg ctggattttt ttataacgtt tcggttgttt
    14941 tttgtaaaat tgctgatgat agttttctgc tggccaaaac gtaccggctg gttcgatttt
    15001 tgtgacgatt ggttggtgga aagttcctga actgtctagt ttctgttttg attgttccgc
    15061 taattctttt tgtcgttcga cttgataaaa aatcactgga cgatattgct tccctctatc
    15121 ttgaaattgt ccaaatgtat ctgtgggatc agtaatttgc caatagattg tcagcaactc
    15181 gctataactt atttctcttg tgtcataaat gatctccaca gcttccacat gacctgtgta
    15241 accaccactt acttgatcat aatttggttt ttcaacatgt ccgcccgtgt atcctgacaa
    15301 aacagatagg atcccttttt tcgattcaaa cggctcgacc atacaccaga aacatccccc
    15361 tgcaaatact gcacgttctt gatagtcagc atcttgcatg gcatgcttgg aaaagtcata
    15421 tttttgctcc ttatcaaatc cttccgtaat caataaataa aagtccatta catcaggcgt
    15481 cagattcatt cggatagcta atggacgtag tgttgcttct agttttgata actgttcttt
    15541 aacggatata ttttctctta tgtcattctt tgtctgaatc aatacttttc gttcccaatc
    15601 tcgtgtattc ggattcaaaa tcagattata aagtgtttga atgaatattt gttcctcatc
    15661 catagattct cctcctttcc gaagaagaca agctagtgac tttagctgta atttatcata
    15721 aagtgaggaa ataagcgatt acttcatccc cctacagatt tttcgtataa aaaagagaga
    15781 atccttatcg acagattctc ctttttaatc aatttatttt ataaatgaaa agttaattta
    15841 ccttttatgg aaaactattt taaaaaataa acataaatat gcagttaatg taacaagata
    15901 agaaataata gtcgttactg tataacttgt gcttacttta atatcagcaa ctcctttgac
    15961 tccgcttatt tgtaccaatc cattagcatt cacctgctcc tttacaggtt gatgatttat
    16021 ttctacattc gttcctaaat aagcatatac aggaagtgtg attacttggc tcttctcatt
    16081 agttactcta tatatgatag aactaccagt tgttttataa ggaaggttta cccattgact
    16141 gccaatcttt gcttcgtgtt ttattatatg ctccttattc tccaatgaag cgataggaga
    16201 ataatcatat aaaaaataat ccattccttt agtggtatac gcctctactg tttctttgtt
    16261 tacaacaata tgtcccggct gagaaattat tttttgattg aaattttttt tactagaaac
    16321 agttaaagtg attaataaca aaacagtcat aaataaataa aatagttctc ttgtcttatt
    16381 gtatcttcta tttttgaaaa atatcatcgt taatatcaga gaagagaata ttatttgcaa
    16441 gcttaagatc cgccaaggaa attgtaacaa tttggctggc gtattttgca gaagatacca
    16501 tggcaccaaa ctagttgtag ctagccaagt caaacacgta ccataataaa tctttttttc
    16561 tatgtttgtt aattttttaa ataaaatcag gggaatacat aaagataaca gaccaactag
    16621 tcccattgag taagtagtta agtcgttgtt catagcttca gtcagactat cgaacacatt
    16681 agatgcatga gcatataaat ttgtcacgct aggtttgttg attttttggt agagcatttg
    16741 ttcaaacatc ggataccaaa aagcactcgt cagaaccaat gtcattcctg ccgcactaaa
    16801 taatttttta aatatagcaa taaaatctgc attcttgaaa cctttagaaa ccaaacgaat
    16861 aaaaataaaa atgataatta atgctgtact cattaatgtt gatagtacat ggctgtacac
    16921 tagcaaactc atactcaatg ctagctttac agacggatac ttttcttctt ttccagagat
    16981 gatttgataa gcataaaata atacagggat aacaagtgtt attgcgatag cttcggcaat
    17041 tgcggaacga tagtagatgt ctactgtccg ataagcagag aaaatataga gacaactaaa
    17101 taaaaatgcc gctttttttt gttttaaaaa ccgctcgcca taatgataag ctacgagtgt
    17161 tgtacacaag ttcaataacc aaacgtaaat aatatagctt acaatcaaat tttttgtcat
    17221 ccaatataac attacagctg gaaaaaaagt cagaaaggga taaaaataat ttacaccata
    17281 tccagtataa ttgaatgtct cataattaat cggactggta aaaatagtcg ataacccctt
    17341 gattcgagat aagtgaaaag aaagatctcc agtggtataa atactctgtg tatttttgaa
    17401 aaagatgaaa gaatatatac tactaactat aattaatcca ctcaatatta ctactttttt
    17461 atttattttc attcttcttg tttatctcct ttttagtcac atatcgagaa tctttcaact
    17521 tattaggtgc aaatcgttta aaacttgaat acaccactaa acaataagtc agaatactga
    17581 tccataaagc catgagagca gttggagtat attttgttgt aatttctatc ttattttctc
    17641 cagcatttac atccacattc actaagccat gttttttata agagtcattt acgactttac
    17701 cgtttacttt tacctgtgta cctaaataag catatactgg tactgtcact ttttgctttt
    17761 tgttgcttgc cactgtgtaa gtaatgttat tatctgcagc tttatacttc cctttttccc
    17821 acgattctcc tacttttact tcatgttttt ctaagtgagg cctttctttt atcgcttctg
    17881 ttggggcgta atcgtataat cctcccatat ctgatgttgt ataacgtggg agtgtttctt
    17941 cattgataac aatatgaccg tgttttgatt ggataacttt tgcgtaattt tcttttgctg
    18001 ctatagacgt gacgagtaga aacaccacag tagcgccaat gattaaccat gaatattttt
    18061 tattcatagt ctttttcata aaaacaagcg ttatgatcat tgaagaaaaa aagatttgga
    18121 tacctaagat acgccaagga aactggagta agcttatcgg tgttttttga aacaaaaacc
    18181 atggaaataa attagttacg catacccatg tagaaactac gccaacatag atgatttttt
    18241 cttttttctc catttctttc agtatgaata aaggaaaaat caacgcaacc aatcctaata
    18301 accccatagt gtatgttgta atatcgttat ttaacgcttg aatgatacta tctgaaatag
    18361 aaagtgcttc tagctgcaaa tctgttttga agggactgtg tatttcttga taattcatct
    18421 gctgcaacat aggataccaa aaatagctag ttaatgcaca agtcaaaaaa acagccttta
    18481 ctaaattcac gaccacaaat tttatatcgt tttttatttg cttgcttaaa aacaaacgaa
    18541 ctataatcat acaagcaatc aatgcactac acaatagtgt agttaagaca tgagtataca
    18601 caagtagact tatactgaca gataaagcga tgactttttt ttctctattg aatatcaatc
    18661 cataagcata atataaaaca gctgggataa tcgtaagagc gattgcttct gcaattgcgg
    18721 atcgataata gatatcaact gttcgataag ttgcaaaagt atataaacaa ctaaataaaa
    18781 aagcagcatc tatcttttta aaaaactttt ctccataata aaaactaatc agaatcgtac
    18841 aaatattta
//
LOCUS       DAZAID010000056        18365 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000056 DAZAID010000000
VERSION     DAZAID010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.056"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..1821
                     /locus_tag="ACG9BE_001803"
     CDS             160..1821
                     /locus_tag="ACG9BE_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3032545.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1891..2253)
                     /locus_tag="ACG9BE_001804"
     CDS             complement(1891..2253)
                     /locus_tag="ACG9BE_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFN3032546.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2321..3262
                     /gene="opp3b"
                     /locus_tag="ACG9BE_001805"
     CDS             2321..3262
                     /gene="opp3b"
                     /locus_tag="ACG9BE_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFN3032547.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3263..4306
                     /locus_tag="ACG9BE_001806"
     CDS             3263..4306
                     /locus_tag="ACG9BE_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3032548.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4312..5382
                     /locus_tag="ACG9BE_001807"
     CDS             4312..5382
                     /locus_tag="ACG9BE_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3032549.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5372..6325
                     /locus_tag="ACG9BE_001808"
     CDS             5372..6325
                     /locus_tag="ACG9BE_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3032550.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6337..7347
                     /locus_tag="ACG9BE_001809"
     CDS             6337..7347
                     /locus_tag="ACG9BE_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFN3032551.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7632..8453)
                     /locus_tag="ACG9BE_001810"
     CDS             complement(7632..8453)
                     /locus_tag="ACG9BE_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFN3032552.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8550..8828)
                     /locus_tag="ACG9BE_001811"
     CDS             complement(8550..8828)
                     /locus_tag="ACG9BE_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032553.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9096..11105
                     /gene="metG"
                     /locus_tag="ACG9BE_001812"
     CDS             9096..11105
                     /gene="metG"
                     /locus_tag="ACG9BE_001812"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFN3032554.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11584..12171)
                     /locus_tag="ACG9BE_001813"
     CDS             complement(11584..12171)
                     /locus_tag="ACG9BE_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032555.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12346..13668
                     /locus_tag="ACG9BE_001814"
     CDS             12346..13668
                     /locus_tag="ACG9BE_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN3032556.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13823..14599
                     /locus_tag="ACG9BE_001815"
     CDS             13823..14599
                     /locus_tag="ACG9BE_001815"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFN3032557.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14592..15161
                     /gene="rnmV"
                     /locus_tag="ACG9BE_001816"
     CDS             14592..15161
                     /gene="rnmV"
                     /locus_tag="ACG9BE_001816"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFN3032558.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15154..16038
                     /gene="rsmA"
                     /locus_tag="ACG9BE_001817"
     CDS             15154..16038
                     /gene="rsmA"
                     /locus_tag="ACG9BE_001817"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFN3032559.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16489..17118
                     /locus_tag="ACG9BE_001818"
     CDS             16489..17118
                     /locus_tag="ACG9BE_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032560.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17242..18351)
                     /locus_tag="ACG9BE_001819"
     CDS             complement(17242..18351)
                     /locus_tag="ACG9BE_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3032561.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         6005 a         3316 c         3747 g         5297 t
ORIGIN      
        1 tattttttct gaaaaattcc ttgaaacctc acgaaaaaat cattatttta tgaaaaagac
       61 aaaaaaaagt gttgattttt taataattat tcattaaact cttaaacaac ttcagaaaat
      121 attaattatt ctgaaaattc caataaaggg gtacgtaaaa tgaaaaagaa atttacattt
      181 ggtttggttg cggtatgcgg ccttgtactt gctggatgtt atggcggcag ttcagccaca
      241 agcagtagtt cagcaaaaag cggttcagca gatggaggag gcgtatttaa cttagtcgtg
      301 cctcaagaaa tgcctacagc tgacttatca gttgcaacag atacaatcag ttttacagca
      361 ttgaataacg tttatgaagg aatttaccgt cttgatgaag acagcaaacc acagccggca
      421 ggagctagcg agttggcaga agtcagtgaa gacggattaa cttacaaaat caaattacga
      481 gaagacgcaa aatggtcaaa tggtgatcct gttacagcag cggattacgt ttatggctgg
      541 caacgaacag tagacccagc tacagcttct gaatatgctt acctgtatgc gccagttgaa
      601 aatgcagaag acattacagc tggaaagaaa gataaatcag aactagggat caaagcagtt
      661 ggtgactatg aattagaaat caaattgacg aaacaaacac catatttcca atatctgctt
      721 gctttcccat catttttccc acaaaaacaa tccgtagtgg aagaaaatgg ggatgcctat
      781 gcatctgcaa gtgataaagc tgtttataat ggaccattca cattagaaga ttttgatgga
      841 ccaggaacag atacagaatg gacttataag aaaaatgatc attactggga caaagataca
      901 gtaaaacttt ctgaaatcaa agtcagtgta gtaaaagaat cttctactgc attgaactta
      961 ttcaaagatg gccaagcaga tgacgtgatc ttgtctggtg agttagcaca gcaaaacgca
     1021 aacgatccag cttatacttc tgttaaagaa gcgcggacaa gctatatcga attcaatcaa
     1081 cgtgaaaaga attcaccatt caacaatgtg aacctaagaa aagctatttc ttattctatc
     1141 gatcgtgaag ctttagtaaa acaagtacta ggtgacggat cagttgtctc tacaggattg
     1201 atcccatcag atatggtaaa aagtccaaca acgaacgaag actttgcaaa agaagctgga
     1261 aaattagttt cttatgacaa agataaagca aaagaatatt gggaaaaagc gaaaaaagaa
     1321 ttgggcgttg attctttaga atttgatttg atggcttctg atgatgattc atcgaaaaaa
     1381 gtaatcgaat atgttcaaaa ctcaatccaa gaaaacttgg atggcgtaac agtcaaacca
     1441 acacctgttc cattctctgt acgtcttgac cgctcaactt cgggtgattt tgacgcagta
     1501 ctaggcggat gggctgctga ctatgcagac ccaagcagct ttactgattt gtttgtcaca
     1561 ggtaattcat acaaccgtgg tcgctggagc aatgcagaat atgacgaagc agttaaagca
     1621 gcttcatcaa cagacgctgg cgatgaacaa gcccgttggg atgatttcca aaaagcaatc
     1681 aagatcatct ctgatgatat gggtgtcgtt ccggtgtacc aaaaagcaga aggtcaccta
     1741 gtgaatgaaa aaatcaaagg tatcgttcac catgcggcag gcgcttcatg ggattacaaa
     1801 tggacatacg ttgaagaata atcgttaata ccgtgagcat gagaaaaaac gaggctgcgg
     1861 caaatgtcgc aacctcgttc tcatattcac ttatcctaac aaagaaaaaa gcatgaaaat
     1921 agcagaaaga gcaattagta ttccggcaat gatcagccaa aagctgtaca taccgtagct
     1981 ggcagacgag agcgtggaaa attctggttt tttctttcgg ttccttgttc gtgcaagatg
     2041 gaccgatcgc tgaaaatgat cgaagaaacc cgaagaaaag acccataaaa aaccgccgat
     2101 aatcagaaac ggcaatgacc ataagaatag atcattagat aattgaatga gtgtaaagga
     2161 atggttgatg agatttttta ttaaaataac cagaatcagt agaaaagcta ctgcgtaggg
     2221 gatagatttt ttttcatttg attgctcctt tatttttggt tcattatcta ataatgtagc
     2281 gaaacatctg tcagaagtca aaagctaggg ggaaacatta gtgaatagtt atatcaaata
     2341 tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg ttaattgcaa caatcacctt
     2401 tttcttgatg caattgctgc cgggcacgcc atatacgaat caggaacgtt tgagtcctga
     2461 aacaatcgag atgctaaata aacaagtcgg tttggataaa ccagttatcg tacaatatgg
     2521 gatttatctt tctaatttgc ttcaaggaga ttttggaatt tccttccaat ttaaaaacca
     2581 gccagtcgca catttgttag ctggaagaat cggaccatcc ttgcagttag ggttacaggc
     2641 aattatcttt ggaacagtcc tagggacgat tttaggaacg atctctgcca tgaagcaaaa
     2701 tacatgggca gatacatctt ctacactcgt agcgatatta ggtcgttcca ttccaaactt
     2761 tgtatttgct gttttattac aatatatctt tgcaatcaaa ttacaagttt tgccaatcgc
     2821 aaaatgggat ggctttgtgt acacaatctt accaacaatt gcgttggcca tgtcgccgct
     2881 tgctgactct gctcggttta tccgtacgga aatggtggaa gtactgcata gtgattatgt
     2941 ggaattagca aaagcaaaag gattgagccg ttgggaaatc gcctttaaac atggtcttcg
     3001 aaatagtttg attccattga tgactttatt aggaccgtta gctgtagcat tgatgaccgg
     3061 atcactggtg gtagaaaata tctttgctat tcctggtatc ggggaacaat tcgttaagtc
     3121 aatcacaaca aatgattatc ctacgatcat ggctgtaacg atcctttact catttatgct
     3181 gatttttgtt attttgatcg tggatctatt gtacggtcta gttgatccaa gaattcgtgt
     3241 ttcagaaggg agccgaggat aaatggaaat gataccaaag cgttatgaaa cagttgcaga
     3301 tatccctgcg gatgaatttc ttccattaca aaaaaatacc gaagaagaac gtgaacagat
     3361 tactgcacct tctttgaatt ttatccaaga ttcatggcgc cgtttaaaga aaaacaaagc
     3421 tgccgttatt tcaatggttt tactattggt catcattttg atctctgtga tcacaatctt
     3481 tgtttctcct catgatccaa ctgcccaaaa cgtggcttat atcaatttgc ctcctcgtat
     3541 tccgggaatc aatatcgatg gattgaatgg aaaagcaatg gttggtggtg aacttgttga
     3601 taaatatgct caagcaaacg tcccttcaaa cgtaaatttc tttttaggaa cagacggttt
     3661 gggacgtgat gtattaagcc gcttatttat gggaatgaga atctcgttgc tgatcgcatt
     3721 tattgctgca ttactagatg taacgattgg tgtagcttac gggttggttt ctggtttgtt
     3781 aggcggacgt gtcgataacg tgatgcaacg ttttttggaa atcttatcag gaattccaaa
     3841 cctcgttgtc atgattttga tgttggtcgt ttttgaacca ggaatttttt caatcgtggc
     3901 tgctatggcc atcacaaact ggataccaat ggcacgtatc gttcgtgcac aaacaatgaa
     3961 attaaaagat caagaatatg ttttagctgg attgacatta ggtgaatcaa gaatgaaaat
     4021 tgcattcaaa catattttgc cgaatatctc tagtgtcatt attattcaaa tgatgtttag
     4081 tattccatct gctattttct tcgaagcctt tttaagcttt atcggtttag gtcttacgcc
     4141 tccgtcagct tcattaggaa cgatgttgag cgaagggtac aagacattcc tgtatctgcc
     4201 ttatctatta tggattccgg cagtaacttt atcggttatc atgattggct ttaacttact
     4261 tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa gagtgaagac gatgacgaaa
     4321 attttagaag tgaaagactt agaaatctct tttgatacgt atgctggaaa ggtacgagcg
     4381 attcgcggtg tcaattttca tttgaataaa ggagaaacat tggcgatcgt tggggaatct
     4441 ggaagtggaa aatcagtgac gaccagaagc atcatgcgct tgctgtcaag caacgccaat
     4501 atagatgctg gtcaaatcct ctttaaaggt aacgatatcg ttggaaaatc agaaagagag
     4561 atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc aagatccaat gacctcttta
     4621 gatccgacga tgcccattgg gaagcaagtc gctgagtccc ttagaaagca taataaagtt
     4681 tcaaaaaaag agggacaaaa agctgcatta gaattgctta aattagttgg aatacccgaa
     4741 gctgagaaac gtatcaacag ttatccgcat cagttctcag gaggtcagcg tcaacggatc
     4801 gtgatcgcga ttgcactggt atgttatccg gaaatcctga ttgctgatga acctacaaca
     4861 gcgcttgatg tgactattca agcacagatt ttggagctgc tgaaagaaat tcagacaaaa
     4921 atcgatacat caattatctt tatcactcat gaccttggtg tagtagcgaa tgtcgctgat
     4981 cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg gaacatcgga agaaattttc
     5041 tacaacccgc aacatcccta tacttgggga ctgcttggtt caatgccgac attagatagt
     5101 gcgaacgatc gattgtatgc tattcctggt tctccaccgg atttattgaa cccgccaaaa
     5161 ggagacgcat tctatccaag aaatgaattc gcgatgaaaa tcgatgcaga acaagaacca
     5221 ccattttttg aactatcaaa aacacacaag gcagcaactt ggctacttgc tccgcaggca
     5281 cccaaagtga caccgccaga agaaatccaa cgtcgctggg ctatctttaa agagaaacaa
     5341 aaacatactt acggtcaagg aggaattgct gatgccaaat aaactacttg aagttaaagg
     5401 attgaaacag tatttcaatg taggaaaaaa gaatgaagta catgctgtaa atgacatcag
     5461 ctttcacatt tatgaaggag aaacattcgg tttagttgga gaatctggaa gcggaaaatc
     5521 cactactggc cgtacgatca ttcgcttgaa tgaacctact gatggtgaga tattattcga
     5581 cggtcaagat ataacgaaga tcaaagataa aaaaggtttg acgaaattcc ggcatgatgt
     5641 tcagatgatc tttcaagatc catacgcttc gttgaatcct cgtatgaaag tcagagatat
     5701 cattgccgaa ggaattgatg ttaatggact agctaaatca tctgaagagc gtgcaaaaaa
     5761 agtcgatgat ctattgagaa ctgttggatt gaatccaagc cacggtactc gttatcctca
     5821 tgaattttca ggtgggcaac gccagcgtat tggtattgca cgagcattgg cagtaaaacc
     5881 gcgttttatc atttgtgatg aaccaatttc tgcactagat gtgtcgatcc aagcccaagt
     5941 ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg acctatctat ttattgcaca
     6001 tgatttgtcg atggttaaac atattagtga ccgaatcggt gtaatgcata acggcttgtt
     6061 attagagatg ggaacaagtg atgaaatcta taatcacggt gttcatcctt atacagaaag
     6121 tttgttatct gctattcctc taccagatcc agaccatgag agaaaacgca gaagaatcaa
     6181 atatcaacct gaaccagatg acggacaagt acgtcaactt cgtgaaatcg caccagaaca
     6241 ttttgtctat gcaactgagc aggaagtccc atattacgca aaaaaactaa aacgacaaaa
     6301 ggaatctctt ctggttgcta actaggaggg acatagatgc taaaagcttt taaattccgt
     6361 atgtatccga cagaagaaca aaaacagcaa ttgattcgta cttttggatg tgctcgattt
     6421 acctataacc atttgctgaa aaagcggcag aaaagttggc aacaaacagg agtagccaat
     6481 ttttcgctca cacccgctac attaaaaaaa gaatacccat tcttaaaaga agtagacagt
     6541 cttgctttag caaatgcaca gcttaatttg gatcgggctt ttcgcaatta tttcaaggga
     6601 cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt ggcaatctta tacgaccaac
     6661 aaccaaaccc gtacgatcta tctggaaaat ggctacttaa agttaccaaa acaaaaagaa
     6721 ctaatcaaga taaatcagca tcggccagtt gaaggatcga ttcgctccgc tacgatttct
     6781 gctcgatata atgaagaatt ttatgtcgca ctactttgtg atgtttcgcc agtaaaaaaa
     6841 gaatctttag ctaaatggat cgggatcgcc tatcatccga agacattgat ccaaacatct
     6901 cgacctctag aagtaacttt accaaaattt caccagacag aagaaaagct gcaacatgca
     6961 cagcggaaat tgaatgtgaa agtccgctcc gctcaccata gaaagatcag gttagatcag
     7021 gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc tttatctgaa acaaaagaac
     7081 cagcgagaag attatcttga acaattatct gggaaattgg tcaaacaata tgactactta
     7141 tttgtagaat cttttccaaa agaagaggca catgctgatt tttctattca cgactggcat
     7201 aaattaataa caaaattgcg ttataagtcc caatggtata ataaaaaatt tcttttgatc
     7261 aataccgacg gagcagaaga aagcaattcc gtaagaaaga gccaagtagt agaaaaaatg
     7321 ggcagacatt cggttatcaa gggataaaaa atgaatgaaa ctgataggag cgttagggtt
     7381 agccttgtta ataaaaagtc ttaatgactt ttgtttctag gaatttttct tttgaagaaa
     7441 agctcactaa ttatgacaaa ccaataaaac aatagcagaa aaagttcgta cgagcttttt
     7501 ctgctattgt taatttttta ttcagaaagt ttacgaatac atcaaaattc tgaaacaaaa
     7561 aacgtaactc acaaagagtt gtttttgcgt tttttcttga atgattaggt ctgaatcttt
     7621 tgctcacaac cttattcatg gtgccccggc ttgagctgat agatttttga aggacggccg
     7681 attcctacag gacgttcacc gatctcatcg aaatattgga gcatagcttt cttaaaattg
     7741 gaatgatcga tcgttttata atcgatccct aagaatttag caaatacttt tcgagcttcc
     7801 gtaatcgtga aatctttacc taaaacttgt agaacttgcg gctcgtgttc cattttattg
     7861 actacacggt tgaatgcttt caagatgatt tgactatggt caaaagccaa agtatctttg
     7921 ccagaagact ctccagtgac taaatcaagc gagatagcca cttctccgct agataaatgg
     7981 atcttgttac tatgacgttc tagtgtgaac cagcgtactt ccttggcatc atctccggcg
     8041 attagcggtt cttctccaat aaaggctaag tagctgactg tgacgaccca gcctcgcgga
     8101 tctcgattag gcgtactgaa tgtatgcagt tgctcaatat tttcttttga aataaccaca
     8161 ccagtttctt cctttgtttc tcttaagacg ctttctccag ttgactcatc cttttggaca
     8221 aagccaccag gcaatgccca tgaatttcga taaggatgcc cttttctttg gatcaacaaa
     8281 actttcagct gatcttcttc tttgttatag cacatcagta cgatatcgac agtcaaagag
     8341 ggcttgtcat attctggacg ttcctgtttt tgataccagt taagaaattc ttcttcactt
     8401 gctttatgtt cataatataa tttttcttct tctttagaag caaaacggtt catctgacca
     8461 cctccattga cagtaaaagt actttttacc ttattgtagc agactctgaa aaaaaaccaa
     8521 ggatttactt gaaaaatcta ggttatcatt taaatatgaa aaataaatgg aagtatgaga
     8581 ttgaataaaa agaaaagcag ataaaacggc aatactttat accgctcttc acgaatggca
     8641 aagaagccac aaataacgat atatacgtca atgataaaac gcaaaatcca agctgctgtc
     8701 ataaaactgt tcaaatgccc atggaggtta aaataaccat acatacgaat ataaaaatag
     8761 ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa gtgtaaaaaa aagatgtttc
     8821 ttctgcatga ttgttccagt cccttactaa gatagatagt ctttattata ccacgtgtga
     8881 ggggagaaat aaaaagccgt tttttagtag atatgaaaca aaacaaaaac ccgcatttat
     8941 tcaacctatt catcctttta aaggatagat tgaacgtaga tagaagtcgt ttatccagtc
     9001 tttttcattt tgaaaaaaag ctaacagcat gcttttttta tggtatacta gcaaggaaac
     9061 gaattcgcat catgcgataa aggaggaaga gccccgtggc agaaaaggaa actttttata
     9121 tcactacgcc aatctattat ccaagcggca aacttcatat cggcaactcg tatactacga
     9181 ttgcatgtga cgcaattgct cgttataaac ggttgatggg ctttgatgtg ttttatctca
     9241 ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc agaagaacta ggtgtaaaac
     9301 cgcaagaata tgtagataaa atggcggctg atgtcaagaa attatggaaa acgcttgata
     9361 tcagttatga taaattcatt cgcacgacag atgattacca taaagcagct gttcaaaaaa
     9421 tatttgatcg tttattagaa cagggagata tttacttagg tgaatatgaa ggctggtact
     9481 ctgtttctga cgaagaattc tttacagaaa cacaacttgc agaagtgtac aaagatgacg
     9541 aaggtaacgt gatcggtggt aaagcgccaa gtggacacga agtagaattg gtaaaagaag
     9601 aatcatactt cttccgcatg agcaaatacg cagaccgtct attggaatac tatgaagaac
     9661 atcccgaatt catccagcca gaatctcgta aaaacgaaat gatcaacaac tttatcaaac
     9721 caggattaga ggatcttgca gtttcacgaa caacttttac ttggggagta ccagtcaaga
     9781 gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt atccaattat attactgcac
     9841 taggatatgg ctccgaagat gaaagtctat tcgaaaaata ctggcctgca gatgttcaga
     9901 tggtcggtaa agaaatcgtt cgctttcata cgatctattg gccaatcatg ctgatggccc
     9961 ttgatttgcc tctacctaaa aaagtatttg ggcatggctg gctattgatg aaagacggaa
    10021 aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat gcttgtggaa cgttatggtt
    10081 tagatgcact ccgttattat ttgcttcgtg ctgttccgtt tgggtcagat ggtgtcttta
    10141 cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc aaatgatcta gggaacttat
    10201 tgaatcgaac gattgctatg atcaacaaat attgtgatgg actcgttcct gactatgcct
    10261 cacttgtcac tccatttgat agtgaactgt ctacgacggc tgctaacgtt gtcggacgtt
    10321 accatgatgc aatggaaaaa atggaattca atacagcatt ggctgaaatc tgggtgctga
    10381 tttctcgtgc aaacaaatac attgatgaaa cagaaccatg ggtattagca aaagacgaag
    10441 aaaagaaagc tgaattgaat agtgtcatga tccatttagc tgaatctttg cgtatcgcag
    10501 cgatattact tcagccaatc atgacagaaa cgccagagaa gatcttcaat caactaggat
    10561 tggactctga aacaatgaat cttgaaggat tacactttgg cgaatttcct tcaggaacaa
    10621 aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga tatggaagaa gaagttgctt
    10681 ttattcaaga aaaaatgtct gaaggtactc aaacaaacga agatacagtc aaatgggatc
    10741 cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat caaatttgat gtatttgaaa
    10801 aagtagaact aaaagtagca gaagtcatca actgtcaaaa agtagaaggg gcagataagt
    10861 tgctgcaatt ccgactagat gctggagaca gccaagaccg tcaaatcttg tcaggaatcg
    10921 ctgaatttta tcctgatcca agcgaattga tcgggaaaaa agtcgtcatt gtagcaaatc
    10981 tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat gatcctttct gcagaagcac
    11041 cagatggctc tttacaagta atcgaagcac caaaatccat gccaaatggc tcagaaatcg
    11101 catagaaatt caaagggggt atgacactag ttgtgtcata cccccttttt ccgaaatttg
    11161 agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc aatcatataa tacagaaaca
    11221 gagaactatc caaaataaat cctagaaaaa gaagtacgag cttcgctgat ttttgttaat
    11281 ttagaataat actaatagga atcgtaaaaa cagaaaaaaa gcataatgat aaagggtcgg
    11341 gcagatttta attgattcat ctggtactct tttttattgt ccttacattc ataaagtttg
    11401 aatgcaatga ctttattaca tctatattta tatctaacag tagcgttgat gtgttaatta
    11461 taagttctat cactttatgt tcaattagtt tgtgctctta tatgtgtgat tgaaaatata
    11521 gataaaaaaa caatcatcgg ctttgacttc gatgattgtt ttctttattt tgtaaaaatc
    11581 ctatcacttg attttatggt aaccttttat ttttgtcttc aaacggcttg gtaagagagc
    11641 agtaagcagt gcaaatggga tcgaaaagac ataccagaaa atcgtacgtc cgataccata
    11701 atgttgatag agctttgtca acatcacgtg taacaagatc aaccctaaaa gcaaggagcc
    11761 agctataaac caatgattat cccaatgcat gacttcacta agtgccatca ctccaaaagg
    11821 atagaaataa aaatagatcc cgactaaggt catccgccag ttctttgtaa atgttccgat
    11881 attcaataaa aagaaaatcg ctgaaagcaa aataccgata ggcggaaaaa gcccaaccaa
    11941 aaaggaatac aacatccccc acaagatcat cacaggacct ttgatcaatg caacgattgc
    12001 gatgaacaaa gtcagcaata ttagtttttg tgataacgtt gcaacagtca ttaatccaaa
    12061 taaaattaat gtgaaccaat gccaatggga ctgctgttgt tctttacggg aaaaatcgac
    12121 ccggttggat aaataatctg ctaatttcgg ataagaacgc ttaaaatcca taaactgttc
    12181 ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa aaaagaaata catccgtctt
    12241 aagagggagt ttttcactga aagtggagaa aaaagaatgg taaattaaaa tattctcagt
    12301 tatactgaaa attataccaa atttcaaaga agaaagagga tgggaatgaa aggcaaacta
    12361 aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta tgggaaccgt gatcggtgca
    12421 ggcgtcttct tcaaaactgc tgctgttact gctagtactc agtctacaag tttgacattg
    12481 ctagcttggc ttttgggtgg tttcttaacg atctgtgctg gattgacagt ggctgaatta
    12541 gctacagcaa tcccggaaac tggtggagct gtaaaatata ttgaagctgc gtatggaaaa
    12601 ttgcccagct ttttattagg atgggcgcaa agcttgattt actttcctgc taatattgct
    12661 gcattatcga ttatctttgc gacacaattg acgaacttat tacaattatc taccgattat
    12721 cttttgttga ttgcagtgat caccgctgtt tctgtgacag ggttgaattt gttagggaca
    12781 aaagtcggtg cttctgtaca atcagtaacg ttgattgtta aattaatacc aattgctgtg
    12841 attgtcatat ggggattatt gaccccagga caaggaactg tccagttatt tccaatcgaa
    12901 gctggaaaag atgttacttt tgtcgaagga ttgagcagtg cgttgttagc aacgttgttt
    12961 gcttatgacg gctggttagg tgtcggtgca atggcagggg aaatgaaacg gccggaaaaa
    13021 gatttgccta aagcaattat tcttggtttg agttttgtaa cagtcgttta tctattgatc
    13081 aattttgttt ttttgaaaac tttgccgatt gatcatcttg ccggtaattt gaatgcagct
    13141 tctgaagcat cagacgtcat ttttggagga atcggtggaa agctggtcac tattgggatc
    13201 ttgatctcgg tatacggtgc attgaacggt tatacactaa ctgggatccg cgtgccttat
    13261 gcgatggctt tagaagacga gctacctttc agtaaacagc tgacaaagct ttcaaagaag
    13321 tttacggttc cttatgttcc tgcggttttc cagttagcag tagcttgtat tatgatgagc
    13381 cttggttctt tcgacttttt aacagacatg ttgatttttg tgatgtggct attcagttta
    13441 ctgattttta tcggtgtctt tattttaaga aaaaaagcgc cggaattgcc acgtccatat
    13501 aaagtaccac tttatccaat cgttccgatt attgcgattc tcggtgctat tttcatttta
    13561 ggtatgacga tgatgacgca aacgaaatta gcattgatcg gtataggtgt taccttgata
    13621 ggtatcccag tctattatca aaaaaagaaa aaacgctcag aagagtaaaa tatttcaaca
    13681 acagacagaa aaagtacgaa ctaggtcctc tagatcttcg tgctttttct atttcaataa
    13741 aacaagttct tctattttga tttttgctac ttctgcaagt agtgcttatg tgctaaagta
    13801 agaatgaaaa ggagagataa acatgatatt tgattctcat acccatttaa atgctgaaca
    13861 gttcaatgaa gatattccag aaacgattca gcgagcacaa gaaatggggg taacgaaaat
    13921 ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct ttgcaattaa gccatgacta
    13981 tccaaatata tacagtatta tcggctggca tccgacagag gctggcagtt atacgaaaga
    14041 aatcgaaaat aaattacaag aacaattgac tttaccaaag gttgtagcat taggggaaat
    14101 tggcttggat tatcattgga tggaagaccc gaaagaagtc caagataaag tatttcgcag
    14161 acagattgct atcgcaaaag aaatgaacct accaatcagt attcatacta gagaagcact
    14221 tgaagacacg tatcaaatcc taaaagaaga agatgttcga gatatcggtg gaatcatgca
    14281 tagtttcagt ggtgattatg aatgggcaaa acgatttctt gacttaggga tgcatatttc
    14341 atttagcggt gtcgtcactt tcaaaaaagc attagacgtc caagaggctg cagcaaatgt
    14401 accaatcgaa cgattacttg tggaaacaga tgccccttat ctggcaccag ttccttttag
    14461 aggaaagcga aatgaaccag gctatactcg ttatacagta gaaaagattg ctgaacttcg
    14521 gaatcttccg acagaagaag tggctgccca aacatggaca aatgcgcacc gattatttgg
    14581 tctaagcgaa catggataag atgaagatcc aagagattat agtcgttgaa ggaaaagacg
    14641 atacaagaag gctgcgggaa gtagttgatg cagatacgat agaaacaata gggtctgcaa
    14701 tcaatgaaga gatcctgatg caaattgaac atgcccaaga gactcgtgga gtgatcgttt
    14761 ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat cattatggaa gctgttcctc
    14821 aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc aaagaaaaag ggaagccttg
    14881 gtgtcgagca tgcaagtgat gctgctattt tagaagcatt gaaaaaggtc gtgacgccag
    14941 acatttccca aacggctgtt tctgaaatca ctagacaaga ccttttagac tatggtttaa
    15001 tagctggggc acaagcaaaa gaaaaacgag aaaaactagg tgacgagttg cgcatcggtt
    15061 acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt tcgtattacg ccgaaagaac
    15121 tcgcccaagc aatgaaaata gtggaggaac aacatgagtg aatatcgtga aattgcgaca
    15181 ccctctagaa caaaagagat tttattgaaa catggttttt catttaaaaa aagtttagga
    15241 caaaattttc tgactgaacc aaatatttta aggaaaatcg tagaaactgc gcaaatagat
    15301 gaacatacga acgtgatcga agttggacca ggaatcggtg cattgacgga acaattagcc
    15361 aagcatgcaa aacaagtcgt agcatttgag atcgatgatc gtttgatccc tgtattggct
    15421 gatacgatgc aaccctatga caatgtgact attgtacatc aagatatttt aaagaccgat
    15481 ttgtcaacag ctgttagaga aacatttcat gaggagcttc cgttaaaagt cgtagcgaat
    15541 ttaccttact atattacgac accgatcatg atgcattttc ttgaatcaga tttggttgtg
    15601 gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc gtatctctgc tgaaccagga
    15661 acgaaagctt atggttcatt atcgattgct gttcaatatt atatggaagc cagtttagca
    15721 ttcatcgtac caaaaacagt atttgtccct cagccaaatg tagattcagc gattttgaaa
    15781 ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg aaaaagcatt tttccgattg
    15841 acaaaagcgg catttcagca aagaagaaaa acgctttgga ataacttgca gcattcgtat
    15901 ggcaaagatg accagacaaa agcatggtta gcaaaaagct tggaaacagc tggaatcgat
    15961 ccaaaacgtc gtggggagac tttgtcatta caagaattcg ccgcattaag caatgcgatg
    16021 tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt tgtgaaaaag ctgtcctgca
    16081 tcaagtaata agaacagcca aacaaaaata gttcctcatg tttttcgttt gtttgggctt
    16141 attaccgcag atgcaagctt ttgaacaccg tttaagaaag aggttgtggg cctgccgttt
    16201 aactctaagt cacaaggaac aatttcaaaa aacagcttct catgtttttg agttagttgg
    16261 tcttattact tgatgcaagc ttttgaccac tattatgccg aacaaggaga tgtgacacaa
    16321 attttgtcac atctcctatt tttgtttctt gatcaaaaaa ttcttatact tgatactcac
    16381 actcattttg tatggattta gaaaagaata tgtgtcttga cagttattag taagtgagtt
    16441 atactaagtc gacatttatg tgtcaagaca tattaggaga gagaaaaaat gagaaaaaat
    16501 tcagttcgaa aactaacgat ttctgcttta ttgattggga tgggggtcat tatcccaatg
    16561 gtcatgccta aaatcatgat tccaccagct tcctttactt tggcgtctca tgtgccatta
    16621 tttattgcga tgttttttac accaggagtt gcagtggctg ttgcgctagg tacgacattt
    16681 ggtttctttt taacaacgcc ggttatcatc gcattgcgag cattgtcaca tcttgtgttt
    16741 gcgttgattg gggcttggta cttgcaaaag catccgagta tcgttttaaa aaatggacag
    16801 tttacttttg ctaattggcg tttccaaggg ttcaattttg tgatcggtgt gattcattct
    16861 ttagcagaaa tgctagtagt cagcatcttt tactttattg gtaatatgcc tgaaacatat
    16921 tattctcaag gatattttta tacagtattc atcttgatgg ggattggtgg gcttatccac
    16981 agtttagttg attataatat tgcttatttt atagcaggaa cactcagcaa atattttgat
    17041 atccccgtat ttacggcagc aaaaaaagaa aatcggaaag agtcgattca agggtacgcg
    17101 aagaaaacga catattagac atgatctctc aaaaaaaaaa ctctcatcta gtaatggata
    17161 ataaatcctc taagatgaga gttttttgtc ctaaaaagag ctgcaccaac atcttctggc
    17221 acagctctaa cagggggcaa tttatggaag tttgatgact tgttctgttt ctttatcaaa
    17281 gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt tcgcctggtt tatggaaatc
    17341 tcttgcatct acttttgaga tgaactcagt agaaccagtt tttgtgtaaa gcatcgtttc
    17401 agctccaagt aattcggaaa cgacaacttc tgatttcact acggcttcag gtgaagcttc
    17461 aagggctact tgttcactgt ggatgtcttc tggacgaata ccgaagataa cttcttttcc
    17521 ttcataacct ttttcaacta atactttgtt ttttccttca ggaatacgaa gcttaagccc
    17581 atgtccatct gtgataacac catttttcaa tgtcacatta aagaagttca tggcaggaga
    17641 tccaataaag ccggcaacga acatattgac gggtgtatca taaacttctt tcggtgtacc
    17701 gatttgttgg ataaatccgt ctttcatgat aacgattcgg tcagccatag tcattgcttc
    17761 tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga cgctggtgaa gtttggcaat
    17821 ctcagcacgc atggctacac gtaatttagc atctaaattt gataatggct catccattaa
    17881 gaagactttt gcatcccgaa cgattgcccg tcccaatgcc acacgttggc gttgtccacc
    17941 agataaagca gctggttttc gtttcaagta ttctgtcaga cctaagatct ctgctgcatt
    18001 gtccacacgt tttttgatgt ctgctttatc atatttacgt aatttcaagc caaaagccat
    18061 attatcaaaa acagtcatat gaggatacaa tgcgtagttt tggaaaacca tggcaatatc
    18121 acggtcttta ggtgccacgt cattcatgac ttttccgccg atatttagtt ctccttcaga
    18181 gatatcttcc agtcccgcga tcattcgcaa agtagttgac tttccacaac cggaaggacc
    18241 aacaaaaacg ataaattcac ggtctttgat tgatagatta aaatcagtta cagaataatt
    18301 ttctgcattg tcatattttt tatatacgtt tttcagagcc atttctacca taatacgttc
    18361 cacac
//
LOCUS       DAZAID010000057        18170 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000057 DAZAID010000000
VERSION     DAZAID010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.057"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..1582)
                     /locus_tag="ACG9BE_001820"
     CDS             complement(74..1582)
                     /locus_tag="ACG9BE_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFN3032562.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1741..1932
                     /locus_tag="ACG9BE_001821"
     CDS             1741..1932
                     /locus_tag="ACG9BE_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032563.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2135..3052
                     /locus_tag="ACG9BE_001822"
     CDS             2135..3052
                     /locus_tag="ACG9BE_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFN3032564.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3045..4118
                     /locus_tag="ACG9BE_001823"
     CDS             3045..4118
                     /locus_tag="ACG9BE_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032565.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4266..4784
                     /locus_tag="ACG9BE_001824"
     CDS             4266..4784
                     /locus_tag="ACG9BE_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032566.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4765..5157
                     /locus_tag="ACG9BE_001825"
     CDS             4765..5157
                     /locus_tag="ACG9BE_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFN3032567.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5233..6168
                     /locus_tag="ACG9BE_001826"
     CDS             5233..6168
                     /locus_tag="ACG9BE_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN3032568.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6301..7080
                     /locus_tag="ACG9BE_001827"
     CDS             6301..7080
                     /locus_tag="ACG9BE_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFN3032569.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7073..7390
                     /locus_tag="ACG9BE_001828"
     CDS             7073..7390
                     /locus_tag="ACG9BE_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFN3032570.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7634..9244
                     /locus_tag="ACG9BE_001829"
     CDS             7634..9244
                     /locus_tag="ACG9BE_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN3032571.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9401..10249
                     /locus_tag="ACG9BE_001830"
     CDS             9401..10249
                     /locus_tag="ACG9BE_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN3032572.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            10480..10959
                     /locus_tag="ACG9BE_001831"
     CDS             10480..10959
                     /locus_tag="ACG9BE_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFN3032573.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            10992..13220
                     /locus_tag="ACG9BE_001832"
     CDS             10992..13220
                     /locus_tag="ACG9BE_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3032574.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            13349..14992
                     /locus_tag="ACG9BE_001833"
     CDS             13349..14992
                     /locus_tag="ACG9BE_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN3032575.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(15065..15610)
                     /locus_tag="ACG9BE_001834"
     CDS             complement(15065..15610)
                     /locus_tag="ACG9BE_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFN3032576.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(15633..16118)
                     /locus_tag="ACG9BE_001835"
     CDS             complement(15633..16118)
                     /locus_tag="ACG9BE_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN3032577.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(16108..16920)
                     /locus_tag="ACG9BE_001836"
     CDS             complement(16108..16920)
                     /locus_tag="ACG9BE_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFN3032578.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(17059..>18170)
                     /locus_tag="ACG9BE_001837"
     CDS             complement(17059..>18170)
                     /locus_tag="ACG9BE_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFN3032579.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         5908 a         3306 c         3633 g         5323 t
ORIGIN      
        1 taaagtctat aaaaaaacga aactatcgat taagctaata gtttcgtttt caacatttat
       61 aagtaaactt tttctattgg taatatagat tttcggaagg gtagacaggt gcgcaggtaa
      121 tatcgattcc tagttttttc aatgtttgtt catcattttt attgaccatc acagtagagt
      181 gggcttgtgc tccctcaagt tcagctagtt tatcataagc aacttgagcg attggattcg
      241 ttactgcact gattgataaa gcgatcaaga cttcattgat gcttagtgca tccaaagagg
      301 aatgaagtgt ctttgatttc aatccttgta ttgtttcaag aactaatggt gacaataaag
      361 gaatgtcatc tgcgatatgg gctaaatgtt ttactgcatt taagataact gccgctgacg
      421 catccatcaa tgaacttgta cgcccggtaa tgatgacttc atctggtaat tcgattgcaa
      481 tcacagcaga aggatctttt gtaggacttt gcgcttgaat cttatctgca tagtcatggg
      541 cagggactac cacgtttcgg tcttcaggac gtaaaccgac ttcttccata atcagtttga
      601 ttcgattaac tgcttcttcg tcgatcaatc cttttttgaa gtcgcattct gtttggaaat
      661 aacgacggat gatttcttgt ttagaggctt cttggacaac ggtatcatcc acgatcccaa
      721 atcctacacg attcacaccc atgtcagtag gagattgata aacagattct ttaccagtaa
      781 ttttttcaat aatacgtttg attacaggaa atgtctcaac atcccgatta tagttcacag
      841 ctactttatt gtaggcatca aagtggaagg aatcgatcat attgacatct tttagatcga
      901 ctgtggctgc ttcataagca atgttcagcg gatgtttcaa cggaacattc catactggga
      961 aagtttcgaa cttggagtaa ccagctgcat gtcctttttg gctttcatga tacagttggt
     1021 tcaaggaagt agcgagcttt ccacttcctg ggccaggagc tgtcactaca acgattggtt
     1081 tagttgtttc aatatactca ttttttccaa aaccatcttc tgagacgatt ttttctacgt
     1141 tgactggata gtcttcgatt tctgagtgtt tatagacctt gatgtttcgt ctttctagtt
     1201 tattgataaa gactttcgtg gcaggctgtc cactatagcg agtgataacg acactgttga
     1261 ttaataagcc ataaccacgt aattcatcga ttaaccgcaa gatatccata tcataagtaa
     1321 tcccataatc accacggatc ttattacgtt caatatctcc agcatagacg caaatcagta
     1381 tttcagcttg atctttcaat ttttgcagca atttgatttt cgcatcttct tcaaatcctg
     1441 gaagtacacg tttcgcatgc ttgtcgtcaa ctagcttccc accaaattct aaatataatt
     1501 tgtcatagtg gttcactcgt tccaaaatat acgctgactg ctcttcaata tatttttctg
     1561 aatcaaaacc aatttttttc actaggttcc ctccttaagt actaaagcat tttttcattt
     1621 ttttgaggat tcgtcaactg gaagagcgaa attcctcaaa aaaacaaaga aaatggttta
     1681 aaccaacttg tctagatagt tattcattgg tatgatagtt tttgataagg aaggtgctac
     1741 atgaaaaaaa gacagaaaaa gaaaaacgca tataaacact atatccgttc gatttttacc
     1801 ggttatgaaa gaatgcttga agatcccgaa ttggaacaat tgacgtttac ttatctaaac
     1861 gaagaaacgc agctaactcg tgatgagcat cagcgcattc atttcacaac aagagatctg
     1921 cctagtaaat aaaaaataag ataaaggtca tcggattcga ttctgtccta tcagatggaa
     1981 agactagttg atcgaaaaaa aggctgtacc aaattttggt gcagcctgtt ttatggagca
     2041 ttgcccgctc ttttttataa caatttttta cagaaaatgg tactattgaa gtattaataa
     2101 gtgaaaaaca gatgagtaag aggaggcaag tagtatgaaa gagccagata caaatgcaaa
     2161 ttatgagcca gacgaactag aggagacgaa tgcggatcag gatacaggac aagaatcgtc
     2221 ttctttttac gatatcaaag aaaaagcaag catgaatctt tcccaattat tagaagactt
     2281 acaggctttt gaaaaagctg tccgcgaaga agatatccct gagatctacc gtttatacaa
     2341 tggccgattg aatgaagaat taaaaagaag ctcaaatgaa aatcacgaga tcgatcaatt
     2401 gctgatgcgt aagatccatg atcgtttttt acaggaattt ccttttatgg aacaagtaga
     2461 aaacatctcg ccaacgatga gctattacaa aatcggcact tattaccatg atcggcccac
     2521 ggtcggtatt gatgcaagtc tgccagaaat ttttgttttg cctaagatcg atgaagagtg
     2581 ggagaaatat tcccaaccgg agtcaacgga ctatgacaaa aaaatcaatg agctagacgc
     2641 aaaggtgatc actgctcaga cagaaattga acgtttagat gaacaaataa aagaaatcaa
     2701 tcgacaaatg actgatttga acgacaataa aggattcttg aatcgtaaaa aaatcgagga
     2761 agaaattcaa gaattagaga agaagaagca agtactatct aatgaaaaat taggctggct
     2821 tccttatatt gagacgcctg aaacaatcca gcagcaaaaa gaagcattga agcaagaagc
     2881 aagagcagac cagcttcgtg cagccatcgt agaaaaagaa caaaggcaaa tcagacgcta
     2941 tttcggtagc aaagagggat tcggtcaagc aatccatgaa ttcttgatga attatcttgg
     3001 caatgagaat cctaataatc aatctaatga aggaggttcc gagtatgaat gaacacgatg
     3061 aagcagaatg gacaaaaaat gaagaacaaa cagaagatca tgaagaaacg aacacttcat
     3121 tggaagaaca aacaagtaat cctctggatg acacagaaga aatccctatg ctagaggaaa
     3181 caaaaataca cgaaaaagaa aaagtaactc cgacgaatac gaatactgca gaaaacaccc
     3241 atgcttacga aataactgaa aatcccgggt atttagaagg aaaagagcaa gatcatcaag
     3301 atatcgtcag tttgattgaa acagcggaag cagaattagc ggatttacgt atgagaaaag
     3361 atttgatcga atcagatttt ccacaattat tagaatcata tgatcgtttt atattgaatg
     3421 aagaaccaat ctccacgatc ggaaagacaa gcctgatcga atacttcacg tatgaattac
     3481 tgaaaccgtt gaatgatatt ggtctggaac ggtcaaattc agcttatgac acatggaaaa
     3541 caagacattt ttcgatcaat tatacgttga atggacaaaa cgaattagtc ttttcctttg
     3601 tattaccgac catcgatcaa cgataccaag tggaatcgat gagtctccta aacgtatcac
     3661 cagagacaat ggaaatcaat gtccaagatg accgggtgct ttctttgatc cgacactggt
     3721 cagtggatcg agtattctct gcagggcaga tcacgatatt caatcacaaa ttgaatcaat
     3781 tattagccca tgcaagaaca cttggtttta ccgttaatca gacattacta gacaatacga
     3841 agccacttcg tttgaatctt cagagcgaat ttgaattgac gaatgaagta ttggatgata
     3901 tttttatcgt ggcaatgaaa aatccatcct acgatatgga aaagatcaac gaaactactt
     3961 atcaagtacg attagataaa ggacagtctt taacgataac aacaaacgaa aaagatcaaa
     4021 cagtcttgtc gatctcatct ggagactacc cacgatctgt cattgatttc tttatcaatt
     4081 acgaattctt ggtcccatta atggtgagaa agtcctgatt ggataagttg gattaccaga
     4141 tgtcattcag ataattctgt tccaaaggac ttgcggcagt tatccagaat aattgtcaca
     4201 agtcttttct gtatgtacta aatggacgtg aataacttgc tgatgaagga gggatttcat
     4261 taggaatgtc cacaaataaa atagcacagc ttagcttatt gtctgctgct tgtgtagcag
     4321 gtaggatcgc ttttcaattt attccgaatt tccagccaat gactgctatt tttttattta
     4381 tagtacttta cttaagctta aaagatgcgt taattgtcat gagtctttct attgcgatca
     4441 gcagttttta ttttggtgtt ggaccatggg tcatcggcca atggatcagc tatactgttg
     4501 ttctgagttt gttttcaatc gtctgcttga gaaaaacagt gcaaaggaat ctatggttaa
     4561 agggagtctg tttttttctg gcgggcatgg tttacggaat aggtatgacc gtttttgata
     4621 cgctgttata tcgtttgcct caaccttgga tctattacct tcaaggggtg tcgtttgatt
     4681 tgatgcacag tataggcaat gttgtgttct ttcttgtttt tctgccagtc gtcaaacgtt
     4741 tttacaatca ttcaggagga aaaaatgaaa aaaataattt atagtttgct tgcaatgagt
     4801 gtcagtatac tcattttgac aagttgcagt acaacctata cacaatcggc ttccaaccaa
     4861 aaggaggaag taaaaacatc gcaaaaagca acaatcacgt tacaagaaaa tggtcaaaac
     4921 ttttcggaaa aagaagtgtt cttcaaaaaa ggcgatgatt tactgagtat tttgaaacga
     4981 aattttgaag tcaaagaaga taacggcttc atcacttcgc ttgaagggca ctcacaggat
     5041 gagaaaaatc aactctattg gatgtttact gtggacggga aaagtgctac tactggtgcc
     5101 aaagagatca aattgaatga tggacaatcg gtcattttca atttgtctaa actataagtt
     5161 tcataaaaaa ctgttaaagg gcgaatattg ccataattca gtgagttcag ttgatgaaag
     5221 aaggaaaata acatggaaaa ttttaaaaaa agcggaatgt tttccgtaat tgctgctcta
     5281 ggggctaata ttctagttgc tatcagcaaa tttgtcggct atgcaatcag tggcagtgcc
     5341 gccatgctaa acgaaagcat ccacagtatc gttgattgca gcaatcagat tttgctgtta
     5401 ttcggtgata aacaagccaa taaaggacag agcgagttgc atcaatttgg tgaaggacga
     5461 gcaaaatact ttttcagtac aatcgtcgca atgatgcttt tcttcggcgg tggggcactt
     5521 ggggtgatgg aagcttttga aaagttgacg catccatccc atgaagtggg taatccgatc
     5581 attgttatag caatactgtt atttggtttg gtaatcgaag gaagttcgtt acgcgtggca
     5641 atgaaagaaa tcaaagaatt gaatactgaa aaactttcca cgatgcgctt tttgcgcgaa
     5701 agtcgacaca gtgaaatact gattattttt acggaagact tgtgtgctgt aatcggtttg
     5761 gtcttggctt tagcaggaac aatattgact tatgcaaccg ggattgctgc atttgatgcg
     5821 atcagcggat tgctgatcgg tttcttgctg atgggagccg ccattttcct agcaaaagaa
     5881 ttttatagtc tgatcatcgg tgaaagcgtg acagcaagcg atttatcaaa aatcaagtta
     5941 gcttttgaac gtcctgagat cagtcggctg atcgatataa aaacagttca tttaagtcca
     6001 acggagattt tagttgcagc aaaagcagat atcgttggta acaaagagaa tgaagcttac
     6061 tcggtcatca atgatattga aaaaaatatg cgtcagtctc ttcctgacaa gaagatgtat
     6121 atatacattg aaacagataa atatgaccca aattattctc gtaaataaaa cgtttattca
     6181 tgaattgaag caaaaatgag aaaaatataa tcttgttttt aaataaaacg gctacaattt
     6241 agcgaattta tgttacaatg agtccgctgt ttatttgttg taattaaagg agatatttta
     6301 atgaacaaaa agaaaagtgc gccatggctt caagctttgc atgcagtgat gccactttgt
     6361 ataagctata taccagtagg attagcttgc ggagtattgt tgcaaaaagt tggatttgat
     6421 cctctcttat caggactgtt atcgattctg gtcttttccg gtggcgcgca gtttttagtt
     6481 gcatctatgc ttacgacgca agcatcattt gcaacgacat tattgatggt tttcttttta
     6541 gaattacgct atacattatt aggagcaagt cttgccggtt ttatgaaaaa agaaaagcgg
     6601 acgtttttgg ctgtcttttc tcaatcactg aatgatgaaa actatgcggt gaactactta
     6661 aagttttcaa ctgacccttc ctggaataaa agaaaggctt tatatgtcaa ttggttctca
     6721 atgactgcat gggccggtag caatatgatc ggtaattttt tcggctcggt gattcgagta
     6781 gatgctgacc ttgtccattt tgctttgacg gccatgttca ttttcatgtt tatcatgcag
     6841 atgaaaagcg cactactgat ctttacaggt ttattctcag gcgtattagg ggtcgtattc
     6901 atggtattat tccaaaatac tttcggattg attgcagcaa cagtcattgc ttcatttgcc
     6961 ggatttatgc tggagaaggc attcaaaaaa gaaaaagcta agaaactaag agaccgcggt
     7021 aaagatgtca atgagccgct cttcaacttc ccggaacagg aggccaatca ccatgactaa
     7081 actatatttg ctacttttag ttgcttgtct gttcatcgtg gcatatatcc cgcgcttgtt
     7141 cccgatgctt tattttactc atcgaaaagt gccatcctgg ttcagtgact ggatgaaata
     7201 tgtaccggtg gcattatttg cggcgttagc ttttaaagat gttttcatta ctcatgaaca
     7261 tctggatatt gcttggaata tcaaaatagc tgcaatgatt ctagtagcag gcgtggctta
     7321 caaaacacgt tcgatggcgt tgtcagtttt gacaggactt gcgtctgtct ttttgctgtc
     7381 tatgctttaa actgttttat tttattaaaa acagagattg cgacataagt tgttcaaact
     7441 gatctatccg aactgcattt gcaacagtca attctggatg caagtttatt cgtagggata
     7501 aaaaagatta gtgttggctt atgatgcaag ctctttttta ttttaggaat tagagaggga
     7561 tttgtccttc tatgattttt tgctataata gggaagatgt ttattttatg aaagaatagt
     7621 tgagggaatt taaatgaata aaaaattgat gcaaggaaca ttttggctaa cctttgctaa
     7681 tcttctatgt aaagttttag gggtagtcta tttgattccc tggctaagta tgatggggaa
     7741 caatcaagat ggtatgcttg ctacgacatt gtataatgtt ggctatcttc cttatggatt
     7801 gttcctgatg cttggtacag tcggatttcc aaatgcgatt gcaaaaaaag tagctgtagc
     7861 cacaaaagaa ggagataacg ctgcttgtcg gctgattttt agaagtacga tcaatatcat
     7921 gtttgtcatc ggtatcgcct ctgctgcact gatgtattta tttgctcctt tactagccgg
     7981 aatcagtccg attgctaacg ttaataatgg gatactagct atcagaagtt tatgcccatc
     8041 tttgattgct atcccaattc ttagtgcaat gagaggatat ttccaaggaa aaaatgatct
     8101 gcggccttat ggaacttctt tgattatcga acaggctgtg cgagtgatcg ttatcttggc
     8161 tggtacttat tacttgcgcg tattgactga cggaaccata ttagaagctg tattgatcag
     8221 tacagtcgct tctttcttcg gtgggttagc tgcgattatc catatgtata tcgtcggacg
     8281 cagaaaagac ttcttcgaac ttaaagattt tttgatttcc agccgttatt tcgaaagaga
     8341 aaaccgatct gcctctgtct ctatcattcg ggaaacacta ccgtttattt ttgtcggctc
     8401 agtcattact atcgttcaaa tgatcgacca agtaacgatg aaaccactgc ttcacttttt
     8461 ccgtccagag atcgcagatc agcaactgga attcttattc agccgtgctt ctgtcaatcc
     8521 aaataaattg acattgatcc tgatctccat ggtaggaaca gtagctataa gcagcttgcc
     8581 aatattaagc acgatgaaga aaaaagatcg gatacaaatc gaaaaaacag taggagatag
     8641 tttttcgatt gcgttattga ttcttcttcc ttcattgact ggaatgtctt tattagctgg
     8701 accactatac acattgtttt ttggctatga tcctgaaagt gtcggatatt tccaaatggc
     8761 tttgctagct tctttgtttt tctctttatt cacgatccta tctacgatgc ttcaatcttt
     8821 gaatcaccat cgttttgcga tccgattaac tgtagaagca attatcctga aagtcatctt
     8881 ccaagtaatc ggattaggat tagttggcgg atacgggatg agcttatcaa atggcttagc
     8941 attcggtatt gtctttgttc gcggctatca ctttatgtgc aaagaatacc gaatagcacc
     9001 tttagcaaag atcagcaatt ttttcttaaa aactttccgc tccaccctga ttatgcttgc
     9061 agtatgtttt gccgttttct ttttgctgaa tcagcgattg tcaatggaat caaaaagcta
     9121 tgcaatgatt tattgtgtgg ctgtaggaag tattggcggc ttagtatttt tattttctca
     9181 attcggaaaa aatgggtttc gaatgttgaa aaatgttaaa catcatagca gaagcaaaga
     9241 ataggttaac taatcgtttt ttgctgtcac ttgaaaaacg gcaactcgta tgattacgct
     9301 tgtcgttttt ctattttcca attttagttt catatctaat cttacttatg atatagttat
     9361 aaggtctgga aaaattcttt gaaacgagga aattaaacac atgaaacttg cagtagttac
     9421 agatagttcg gcatatctgc cggaacgtat caagaaccat cccgacttat tcgttattcc
     9481 aatccctttg attatggatg ggaaaattta taacgaagga atcgatatcg aagcggatga
     9541 atattatgga cttttaaaaa acagtaaaga ttttcctact acttcacaac ctgtcgtagg
     9601 ggaagttttg ggtttatatg aggaattgaa agagaaaggg tatgacacga ttttaagtat
     9661 ccatctttct tcagggattt ctggctttgt caacacatta tttgcgatga aagatgacat
     9721 cagcggagta acggtcattc cttatgactc taagatcact agtatgccga tgggacacat
     9781 ggtagaagct gccttagact taaatgatga aggtcagtcc cttgatgaaa tacttgcaca
     9841 tatcgatcga atccgagata atacctacgc ttatctgatt gtagatgatt tgaacaattt
     9901 agtacgagga ggacgtttga caaatggtgc agcgctgatt ggcagcttat taaaaatcaa
     9961 accgatcctg acattttctg aagggaaaat tgttttattc gaaaaaatcc gttcaagtaa
    10021 aaaagcattt gctcgagctg aagaaattat tggtagaaga aataacgaaa tacaaccgcc
    10081 agtcaaactt tacgtgatac atgcaaataa tttagaagta gcgattgcgg agaaagagaa
    10141 attgcagaaa aaatatccaa atgcagaaat cgaaattggt catttcggac cagtcattgg
    10201 tacacattta ggtgaaaaag ccatcggcat tgccatttca gcgcaataat tccttttatt
    10261 caaaaaacat accaaaaata ttccaacatc atcttggact ttctacatgt tactaatgag
    10321 cgtgtaaaag aacgagatgg tgtttttatt tctatcaatg aagctctttt tctttgcaat
    10381 cctcacttca atcctctata atgtcgcata tagtttagta aatgagtaaa ctactaattt
    10441 acttatttac taaaaataaa aggatgagga gttaaaataa tggaaatacc aacaaattta
    10501 aaacataaac cagtaatcgt tgtagaagac tatgataaag ttgatggaag aaatgcatta
    10561 aatacggatg caaaagggct ttcactgggc cttgctcagt ggaatgatcg tggaaaagtc
    10621 gatatctcag gcaaggtatg gcgacatact ggagaaaaat ggtcacgaca gtcggaagaa
    10681 ttaccaatca cacgtatttt agatttagcg atcttagctg cacaaggaag tttatacttc
    10741 caagatgctt atcgttatga aaaattttat gatccagaaa atccagtcgt agacatcatt
    10801 ggattacaag ggaaccgaat gacggtagaa gtagatacca aaaatccaaa gatcgatgaa
    10861 gacatcattt tgtattacga tacactacaa aaagacggag aactgattgg ggaacgattc
    10921 cgtattttaa aacgtctttt agatgagcta ggatattaat agacagagat gatcaaaagg
    10981 ggaagagaag catggagaat ttctcactaa cagaaaaccg aaccttttca cggctagaaa
    11041 agcaaatgat ctggaagaaa ccgaaaagcc atcggacaag tgcagaagaa tggcggatcg
    11101 cctccgaaat caaagggaat tggcaagatc ctgaaatgaa aatcagtaac gtacttttgg
    11161 aaggagatgc cgggtcgggg aagacacaat tagccaaagc actatctgct gatctgcaac
    11221 ttccctatac aaaagtgacc tgttttgcag atatggacaa atcagatgtt ttcggagcct
    11281 tattacctgt agtagattct aataaggaac aagaccaaga attactagaa gctatttacc
    11341 aaacagatac acttgaagct gtactgacac tgatccagaa ccattacggc attgatcctt
    11401 tttctgctaa agaaaaatta gcagagctag ttcaaaggat cgaagctgat tatccagatg
    11461 caatccatta cagctattac ccttctgaaa tcgttcgagc gattgaaaaa ggatatttat
    11521 tagagattca agagccgact gtcatccgag atgcctctgt tcttgtggca cttaactcag
    11581 cgctcgaacc aaatgggatg ttgaatttac cgacaggaat cattcgtcgc catccagatt
    11641 gcgtcattgt gatcactaca aatcgaaatt accaaggaaa tcgcccatta aatgaatctt
    11701 taagagatcg gatgcaacat gcggaaaaaa tggatcttcc tgccttagaa gtcatggcag
    11761 aacgtgcgat ggcgaaaact atgatccaaa aacaagaact tctcttaaag atggcagaga
    11821 ttatccgttt gttggatgaa actgcaaaag ctaacgccat caaaggtgta gcaggaatgc
    11881 gttcttattt ttattgggca aatacctata agcaaggaca agatttactc cagtcgattt
    11941 atccaaaagt tttgtataaa ttatccactg attcagatga attggcaatt cttgaacaag
    12001 ctttgtctga cagtggattg ctactggaac ttcaggacat attgagaaaa gaaaaatggg
    12061 gtcatcctca atctgagcat acaaaaggaa gaaccattag cgccgaagaa gcaaatgaac
    12121 gaaacatcac tgaaaatgct gaagaaattc tgcgcttaga tgaaaagaaa tcgttgtctg
    12181 ctgaagaacc aaatgaggga gaagagaaac aagaagaaac aatagaaaaa aacgagattc
    12241 ctcctcaaat gcaaaaagaa cgcagtagcg atatcgaagg aaaaaatcaa aaagaacaac
    12301 aaacaggaga tgattctcaa gaaatgtctg cttctgacat ggaagcacat gataaggaaa
    12361 tcaagaagca actgaataaa gaagcgcgac aaatcatgaa ggaaacgatt catgaaaaag
    12421 aaggtctcat cgttcaccga ccagctttcc aaggaaaaac gccggaagca cgtgagctac
    12481 acatgcaagt aatgccaata gtagaatcac ttagccggca aattttagag ttactggata
    12541 atgaacaaac agagacgtac caaaaaggaa aatacgaagg tcagcggttc aatgcaagca
    12601 aagtagcata tggtgatttg agaaattttg acaaaaagaa cccgccacat gagcaacctt
    12661 ctttagcggt agctgtacgg atcgatgaat ctgggtcaat gatacgggat gatcggatcg
    12721 aagcggcaaa aaaagcagcg attgccattg ccgaattttc taagaaagtc gatatcccat
    12781 tgttgattta cggagataca gcagaccgtt cgtcaagaga aaagacttct ttgttttctt
    12841 ataaagaatt tgaagacgga tttcaatggt taaatgaaaa attagtcact atgaaaccaa
    12901 ggcaaaacaa tcgtgatggt gctgcacttc gtttagcagc agaaaaatta aacagacaat
    12961 ccgcaacaac caaactgttg cttaatatta gtgacggtca gccaaaagcg ttgccggatt
    13021 atacaggcaa aaaagcaaaa gaagatatcc agtctgtttt gaaagaatat gaacgtcaag
    13081 gaatcctatt tgtctccgca gctattggcc aagacaaaga agaaatcaag tcgatctacg
    13141 gtgaaagtcg ttttgtagat attactgact tgaatgaatt tcccaagcaa atgattcaac
    13201 tgattgcccg ctatctatga taaaataaag ctcaacgaac gcacttagca gattcgttga
    13261 gcttttcgtt tctagttacc ggtaacaagg atacttctaa aatagaatcg gttataatca
    13321 agagggaccg ttaaaggagt gaaacgatat gatacaagaa caaaaatggt ggaaagaagc
    13381 agtaggctat caaatctatc ctgctagttt caaagatagc aatggcgatg gtcaaggaga
    13441 catcaatggg atccgtgaaa aacttaatta tttgaaagat cttggtatcg gctttatctg
    13501 gatcaatccg atctatcaat ctccatttgt cgataatggt tatgacgtaa gtgactatca
    13561 agatatagaa gagagattcg gaacgatgga agattttgat ttattgctaa aagaagcaca
    13621 tgaattaggg atcaaagtga ttatggatct tgtcatcaac catagttcgg atcagcatca
    13681 atggtttgaa gaatcgaaaa aatctaaaga aagcccgtat cgcgattatt acatttgggt
    13741 cgacggggta gatggcaaag aaccgaataa ttggacatcg attttcggcg gttctgcttg
    13801 ggagtattct catgaaactg gacagtatta tctgcatgtt tttgcaaaag aacaaccaga
    13861 cttaaattgg gaaagcgaga aattgaaaga agaactattc aatatgatcc gatggtggtt
    13921 ggataaaggg atagatggat ttcgtttaga tgcgatttct catgtaaaaa aagatgaata
    13981 ttcagtaaaa gccacagaga accctttctc accctttcaa aatgtctcag gaatcgaaga
    14041 acatttgacc gatttgaaac atgtttttga agagtacgat atcatgactg tcggagaagc
    14101 aagtggtgtc actgcagaag aaggaccaca atgggtaggg aaagacggtt atttcgatat
    14161 gatattcgaa tttgatcata tccatatctg gcagcaagaa aaagaagggc aattagatgt
    14221 ccttaaattg aaacttgcat taagcgcttg gcaaacttca ttggatggta tcggatggaa
    14281 cgctttatat atggaaaacc atgatgttcc tcgtgccgtt tcagtgtttg gagatactcg
    14341 tcccgatttt tgggctatgt ctgcaaaagc aatcgctatg atgtatttct ttttgcaagg
    14401 tacgccattt atttatcaag gacaggaaat cggaatgacg aatatgccct ttgaatcgat
    14461 cgatcaagtt gatgcagtag attcgaaaag actttataaa aggctgcttg cggaaggtaa
    14521 gacaagagaa gaagcattgg atatcattcg tgaaacaacg agagacaaca gcaggacacc
    14581 catgcaatgg acttcggaac aatatgctgg tttttctaca catgaacctt ggctgatcac
    14641 taatccaaat accaaaacga tcaatgtaga acaacaagaa tatgaaccag agtcggtcct
    14701 gcaattctat aagaacatga tccgcattag acaaaccaat aaaggtctga tatacggttc
    14761 ttataaagaa tatcttcatg aacatccaca actttatgtc tatgaaagat atttagaaga
    14821 tgaacaatat cttattatgg taaacttaac agaatcactt gctgactatg aacttccaaa
    14881 agaagcagat cagtcctgga cattactctt gtcaaattcc tctagcggag aatttgaagc
    14941 taaaggcata ctagcacctt atgaagctcg gttatataaa acaaacaagt aaaacttcct
    15001 cgttaaagtt cattagttgg actttgcgag gaagtttttc agattgctct gtcaattact
    15061 catttcaata attcgctcta ggaccaccga tatatttagg acgagagcga agggctgtca
    15121 cgtgagcttg tccaagggtt ttcaaacgag gtctgacagg tacttggaca tgtttgatat
    15181 gcataccgat tgatgtgtca ccgatatcga taccggcttg tccaactacg tgttccactt
    15241 cgacaggatc attgaactgg tcaaaagcag caacagaaca agcacctcca gcatgtagtg
    15301 caggaacaac agagacaatt tcgaaatttt ttgcttctgc cactgttcgt tctactacta
    15361 atgctcggtt caaatgttca caaccttgga cagctagcgc aatttcttta ggatccaaca
    15421 actcttttaa cgtacggatg atccattgac caacttccgc actggaattt tttccaatat
    15481 gtcctccaac gacttcgctt gtgctgcatc ctaaaacaaa aagatcacct tttttcaagt
    15541 gtgcctcagc caatatgtca ttaactattt cagtcaattg gtcttttaat tctttttctg
    15601 acaacttcat tttctaaaac cttctttttc aattaatatt gcttccgtgc ttgaatacgt
    15661 tgaagacttg cagttatagg aaaggcaagt cccattccaa tcagattttg gaagatgttt
    15721 cctggtatag aagccactcc agccggccaa tcatataaaa tccaaccggc gataccatag
    15781 ccgatgatca taactaagct tcctaataaa agccctaatc cccacatacg agtagaagat
    15841 ttgctgaaag aaccagcaac atatccttgt aacccgtgga tcactaatga aaaaactgcc
    15901 cactgcggat atcctgaaat caaatcgatc aatagaccac tggaagcacc aacaactaat
    15961 ccacccgttg gaccataaag aaaagcagcg gtatagatcc cggcctcgca taatgtcaca
    16021 aatccattcg tagccggtac agggatcaaa attgttaatg aaagggcaac tgtcaacgca
    16081 atcagcatag catagatcgt taattgctta gttttcattt ctcattcctc ctttttgaat
    16141 accgccagac cagacacttc cggtatgatc gttcatcagc acgccattag aaatacaatg
    16201 ggcaacaaat tttttacttt ctgctactgc tttggtaaaa tctgccccta acacaagatt
    16261 cgctgttatt gcagaagaaa aggcacaacc ggccccgttt actgttgtag tgtcgatttt
    16321 tttatgagaa aatacctcat aatgatcaat tgaatagaga agatccacag cttcatctcc
    16381 agccatcctt gtgccgcctt tgatcacaat gttttttgtt cctgaagcag tgagttcttt
    16441 agcgagatgg atcatttctg ttaacgaaga gggaagagct tgaccagaaa gtaaagcagc
    16501 ttctgccaaa ttaggcgtaa tcatcgtaac aagtggaaaa agttcttcga tgagctgcga
    16561 aatatacaat tgattgatca cagcttcagt ttctttgaat gccaaaacag ggtctaaaat
    16621 gattgggata ttttgttttg tttttatgaa gtctcgaaca accatgattg cttcacttga
    16681 attcaataac ccaattttga taccatctaa tgaagtcatt ttatcaatcg aaactaactg
    16741 tcgtttaagc aattccaaag atatagattc cagcataaat cgctcattaa tatctagtgc
    16801 ccctatagaa gtcaaggcgc ttagtccgaa tagaccaaaa ttctcaaaag tttttaaatc
    16861 tgtttggatt cctccaccag caaatggatc agaaccgcca attgtcaaga ttcttttcat
    16921 aacattttcc tccatgaata agagtataac gaaattttca aaaaagaaaa acagccaatt
    16981 ggcatatttt tatctcatcc aatttaaaaa aacaaaaaga aaagaaagac caaaatggat
    17041 ctttctttcc ttaaatagtt aggacttttg tgattttaat tctttctcga tttgaagccg
    17101 ttgcactttc attgtagctg ttcgcgggat tgcatcaaaa tcacggatga tagggacgtt
    17161 caagcgagga agttcatgga cttgctccca ccatcgattc caatccattt ctttatcagg
    17221 agctaaagca ataatcggtt gtggagaatg atttttatcc cttacaatca ctacttctgc
    17281 caaaaaatct aacgaatcta ataaaaagtc ttcaatagct aatgtactat tgatcgtttc
    17341 gatcaaatcc acttgccgat ctttcaaaaa taggtggcca tgcttatcca taaaaccgta
    17401 atctccgcta tcccaccaat cgccataaac attttcctgg aaccgtgcat cttccttgta
    17461 ataagtcaaa gcccgtcctt tagacagtaa ttgtatatga ccatctgtgt tttcaggtag
    17521 gacattccca actgaatctg taatccgagc gcttgtcatg tcttccagtc caacgcccat
    17581 atctcttgca tcacttgttt tcaaactctc tagtgtatga gccttaagaa tcattggtcc
    17641 gcattcactt tggccatata cttgaagaaa taccgcatcc tgtgccatcg acgttcgcaa
    17701 aaatgtcttc attgtctggt tgtttatagc atcaaaagtg gagtgatagt atcggatacc
    17761 cgaaaaagct tctggatgct ccttagcagt aaaacgccac tgaacaaagt tattcggatg
    17821 ggtctctagt gcaattggcc ggtgagcttc caacatatga acaacttttg aactttgtgc
    17881 gtttgctaaa ggcatcatag gaaatcccat ggacatcaaa gaagagatac caatattgaa
    17941 tctggaatgc actggagaga tgtgaaaagc gattaatttc ttttccgaaa tttttgtgaa
    18001 aactgttttt tgccatttcg tccgccatcc catagaatga gcagaatgac aaataagttt
    18061 agggatacca gttgtaccag aagtatgggt catataagaa atttcatttt tgtctaaaaa
    18121 tgattgagaa acagacaaag caggttgttc cattaaataa agaataggaa
//
LOCUS       DAZAID010000058        17730 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000058 DAZAID010000000
VERSION     DAZAID010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.058"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..831
                     /locus_tag="ACG9BE_001838"
     CDS             19..831
                     /locus_tag="ACG9BE_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFN3032580.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            828..1628
                     /locus_tag="ACG9BE_001839"
     CDS             828..1628
                     /locus_tag="ACG9BE_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFN3032581.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1789..3057
                     /gene="rseP"
                     /locus_tag="ACG9BE_001840"
     CDS             1789..3057
                     /gene="rseP"
                     /locus_tag="ACG9BE_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFN3032582.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3125..4834
                     /locus_tag="ACG9BE_001841"
     CDS             3125..4834
                     /locus_tag="ACG9BE_001841"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFN3032583.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5046..9398
                     /locus_tag="ACG9BE_001842"
     CDS             5046..9398
                     /locus_tag="ACG9BE_001842"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFN3032584.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9542..10015
                     /gene="rimP"
                     /locus_tag="ACG9BE_001843"
     CDS             9542..10015
                     /gene="rimP"
                     /locus_tag="ACG9BE_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFN3032585.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10039..11214
                     /gene="nusA"
                     /locus_tag="ACG9BE_001844"
     CDS             10039..11214
                     /gene="nusA"
                     /locus_tag="ACG9BE_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFN3032586.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11236..11529
                     /locus_tag="ACG9BE_001845"
     CDS             11236..11529
                     /locus_tag="ACG9BE_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFN3032587.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11526..11837
                     /locus_tag="ACG9BE_001846"
     CDS             11526..11837
                     /locus_tag="ACG9BE_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFN3032588.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11850..14156
                     /gene="infB"
                     /locus_tag="ACG9BE_001847"
     CDS             11850..14156
                     /gene="infB"
                     /locus_tag="ACG9BE_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFN3032589.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14183..14530
                     /gene="rbfA"
                     /locus_tag="ACG9BE_001848"
     CDS             14183..14530
                     /gene="rbfA"
                     /locus_tag="ACG9BE_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFN3032590.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14638..15142)
                     /locus_tag="ACG9BE_001849"
                     /pseudo
     CDS             complement(14638..15142)
                     /locus_tag="ACG9BE_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15307..17670
                     /locus_tag="ACG9BE_001850"
     CDS             15307..17670
                     /locus_tag="ACG9BE_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFN3032591.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6045 a         3147 c         3907 g         4631 t
ORIGIN      
        1 agtataggag gaaaaagaat gttacgtttt tttccgcaaa aaaacaaata tattcaagaa
       61 aaaagcgaat ttcaatttaa taccgatgga ccaattcctc agcacattgc catcatcatg
      121 gacggcaatg gtcgttgggc gcaaaaccgt cgtttgccac gagttgctgg ccataaagaa
      181 ggcatggaaa cagtaaagaa agtcacaaaa aaagcatcaa ggcttggagt gaaagtgttg
      241 acgctttatg ccttttcgac tgaaaactgg aagcgaccaa aagatgaagt aagttttctc
      301 atgcaactac ctgtcgactt ctttgatacg ttcgtaccag aattgattaa agaaaatgtc
      361 aaagttcatg tcatgggtta tgaaaacgta ttgccagaac atacgcaaga tgctgtaaga
      421 cgtgcaattg agcaaacaaa aaacaatact ggaatggtat tgaattttgc tttgaattat
      481 ggaagtagag cagagatcgt gactgctgtc caagaaattg ccgaagaagt agcaaaagga
      541 gaaatacacg cagaagaaat cgatgatgaa ttgattgcta agcatttaat gacaggtttc
      601 ttgcctaaag agttacaaga tcctgagctg atgatccgta caagtggaga agagcgaatt
      661 agtaattttc tgttatggca gatcgcatat agtgagctgt attttacaaa agcgctttgg
      721 cctgattttg atggtgcgca tcttgaagaa gcaatcgcct cttatcaaaa tcgagaccgt
      781 cgattcggcg gtgtaaaaaa aacagaagat gagaaagggg atcagtcatg aaacaacgtg
      841 tgatcacagc agctgtggcg ctagtcttgt ttattccgat tatatggatc ggaggcatag
      901 cggttgagct tacagcagca atcttagcgg tagtaggagt ttacgagctg ttccggatga
      961 aaggattgac actcttaagt tttgaaggag ttttatccgc cgttggtgca gtgttacttg
     1021 ttttgccaaa agaaagatgg tttttctttc ttccagagaa tgtaagtaca tttatgttat
     1081 tttatcttat agttatgatt cttttaggaa catcagttat ttctaaaaat acgtatacga
     1141 ttgatgaagc aggttttcct gtgcttgtca gcttgtatgt gggaataggt ttccagaatt
     1201 ttgtcaatgc acgaacagaa agcttggctg ttttgatatt tggtctattt attgtttggg
     1261 caactgatat cggagcatat atggttggtc gacgttatgg tcagcataag ttaagtcctg
     1321 atatttcgcc caacaaaacg attgaaggtg cgctcggtgg tatcggtagt gctgttgtag
     1381 tcgcatttct ttatttattg ctatacccag caaaagaact attcaatcat ggtatgtttg
     1441 tcatgcttct gatgacgatt attttttcag cagtcggaca atttggtgat ttggtcgaat
     1501 ctgcaatcaa acgtcattat gatgtgaaag actcgggaaa tatcttacca ggccatggtg
     1561 gaatcttaga ccgtttcgac agtttgctgt ttgtatttcc tatcatgcat ttatttggca
     1621 ttatttagta agtatacctg cgtttctttg tgaatttcat aaaagacgca ggtttttatt
     1681 tttcattata ttgatactat tggtgtcttt ggtatactat ggtaaaatta tagtgtttta
     1741 tcgttgtttt ataaaaaaca caaaaaacaa gtaaaggaga aaaaagctat gaaaacgatt
     1801 ctgacattta tcatcgtttt tggtatatta gtgattgttc atgagtttgg tcatttcttc
     1861 tttgcgaaac gatcaggaat cctcgtccgc gaatttgcta tcggtatggg tccgaaaatt
     1921 tatgggcatc aagcaaaaga cgggacaaca tatacgcttc gcctattacc tattggcggt
     1981 tatgttcgaa tggcaggcaa tggggatgat gaaacagaaa tggcaccagg aatgcctctt
     2041 tctcttttac taaattctga cggcatcgta gagaagataa atttaagtaa gaaaatccaa
     2101 ttgacaaatg ctatcccgat ggaattgagc cgttatgatt tagaagatga gctgactatc
     2161 actggttatg tgaatggaga cgaaacagaa gtagtaacct accctgttga tcacgatgcg
     2221 acgattattg aaaacgacgg aacagaaatc agaattgcgc caaaagacgt acagttccaa
     2281 tcggctaaat tgtggcagcg tatgctgacc aactttgctg gacccatgaa taactttatt
     2341 ttggcaatcg tgttattcat cattctggca tttatgcaag gtggtgttca agtgacgaac
     2401 actaatcgcg taggagagat catgcctaat ggagctgcgg cagaagcagg cttaaaagaa
     2461 aatgatgagg tagtcagtgt ggacggaaaa gaaatccatt catggaatga tctgacaaca
     2521 gtcattacga aaaatccagg taaaacatta gactttaaga tcgaacgaga aggtcaggtg
     2581 caatctgtcg acgtgacacc gaaatctgta gagtcaaatg gcgaaaaagt tggccaactt
     2641 ggtataaaag ccccaatgaa tacaggtttt atggataaga tcattggagg gacgcgtcaa
     2701 gctttcagcg gttcattgga aattttcaaa gccttaggat cactatttac aggtttcagt
     2761 ttagataaat taggcggacc tgtcatgatg taccaattgt cttcagaagc agcaaatcaa
     2821 ggtgtaacaa ctgttattag tttgatggcg cttctttcaa tgaatctcgg aatcgtcaat
     2881 ctgcttccga ttcctgcctt agatggcggg aaattagtct tgaatatttt tgaagggatt
     2941 cgcgggaaac cattaagtca agaaaaagaa ggaatattga cattagctgg ctttggattt
     3001 ttaatgttgt tgatggtgct agttacatgg aacgatattc aacgattctt tttctagaaa
     3061 aagttgacaa atagggtata ataacaaacg ataactattt tgaggaaata aaggagtata
     3121 tagaatgaga caatcaaaaa tgttgattcc aacattacgc gaagttccaa atgatgcaga
     3181 agtattaagc catcaaatct tgctaagagc tggatatatc cgccaagtat cagcgggtat
     3241 ttattcatat ctgccattag caaatcgtgt attggaaaaa ctgaaacgca ttatgcggga
     3301 agaatttgaa aaaatcggag cggtagaaat gctgatgcct gccatcttgc cagcagaact
     3361 ttgggaagaa tccgggcgtt acgaaacata tggaccaaac ttgtatcgtt tcaaagaccg
     3421 caatgatcga aaaatgatcc ttggaccaac tcacgaagaa acttttactg atttgatgcg
     3481 taatgaaatc aattcatata agaaattacc tttaaatctt tatcaaatcc aacctaaata
     3541 tcgtgacgaa aaacgacctc gcttcggttt gcttcgcgga agagagttta tcatgaaaga
     3601 tgcatattct ttccatgcaa cggaagaaag tttggacgag acatataaag attacgaaaa
     3661 agcctatacc gaagtattca agcgttgtgg cttagaattt cgctcgatca tcggagacgg
     3721 cggcgcaatg ggcgggaaag actcaaagga attcatggcg atctcagaaa tcggggagga
     3781 tacgatctgt tattcgactg aaagtgatta tgctgcaaac ttggagatgg ctacaagcta
     3841 ctatgtatca aaaaaatcgc atgaaacaca gcttgaattg gagaaagtag ctacacctaa
     3901 cgtaaaatca attgacgaag tagcagattt cttttctgtt gaacctcaaa aaatcatcaa
     3961 gtctgtattg ttcattgcgg atgaacaacc tgtccttgtt ttggtacgtg gagaccatga
     4021 agtcaatgac gtaaaactga aaaacttttt atctgctgac tttttagaag aagcaacaga
     4081 agaagaagca caaaaatatc taggtgctga gtttggttct gtcggaccag tcggtgtttc
     4141 agatgacgta aaagtatatg cagaccgtta tgtccaagat ttagcgaatg ccattactgg
     4201 agcgaacgaa acaggattcc actataaaaa tgtgaatcca aaacgcgatt tcactgtgct
     4261 ttcttatgaa gatcttcgtt ttgtacaaga aggagatcct tcaccagaca acaacggtgt
     4321 tcttgccttt actcgaggaa tcgaaatcgg acatatattc aaaatcggca ctcgttacag
     4381 tgaatctatg ggagcaactg tactagacga aaatggacga gaaaaatttg tgattatggg
     4441 ctgttatggt atcggtgtaa gtcgtttatt atcagcaatc gtagaacaac attcagatga
     4501 acaaggaatc aattggccaa aaggaattgc gccatttgat ctgcatatcg tccaaatgaa
     4561 tctaaaagac gaataccaaa caagcctgac gaatgaacta gaagaagcga tgacaaaagc
     4621 tggctatgaa gtactggttg atgacagaaa tgaacgcgca ggagtgaaat tcgcggattc
     4681 tgatttgatt ggctgtccaa tcagaatcac tgtcggtaaa aaagcagtgg atggaatcgt
     4741 agaagtgaaa atcaagaaaa ctggcgaaat ggttgaagta cgtaaagaag aattggcgaa
     4801 tactttacca atcctcttga accaaggcga ataataagcg ttaaaacaca gaaaataaaa
     4861 atagctgaat cagctgtttt tgacaatctg atgattcgct tttaaaattg gaactgcgat
     4921 ttgaatcgca gttccaattt tttaatgtaa aactcagaaa aaacgagtat actaaaaggt
     4981 actgaaaaag aagaggataa gatgcgatca attgcattgt ttctcatcag aaagggcgta
     5041 tatctttgtc agaaaaacga gatttatttg aaaaactgtt ggaacaaatc caactagagg
     5101 aagcagaaaa aaatcatcca ctgcttactg ctggtgaaat ggatagtgta gtggtccatc
     5161 gtaaatcgcg tttatgggaa tttacgctac atttttccaa aattttgcct attatgctat
     5221 atcggtcatt gactcagcat ctgactatcg cttttaaaga tattgcacaa gtcaaactga
     5281 atatcttagc agatgaccag acattcgatg aacagctctt gcaagattac tgggcccaag
     5341 ctttggaaaa tcagcaatgc gatacaccac tagcacagca agtattgaaa acgcaggtac
     5401 ctgtgataaa agatcgtaaa gtcatcttgc caatcgacag tacaggagct atttcttatt
     5461 tgaagcaaca atacttgcca ttagtagaag agttgttcgt cagttacgga ttcccaaaat
     5521 ttcgtatcga accagaagtg gatgagcagc aagcggaacg tgtattgaaa ctttttgaag
     5581 aacgcaaaca agaacaggca gaagccttta tgaagcaagc agctgaaagt ctagttgtcc
     5641 acgaacaaaa gaaaaaggaa agaaaagagc aatcgccagc tcttgaagga cccatccagc
     5701 taggacgaaa tattccagcc gatgaaccaa cgacaccaat gatcagtatc gttgaagaag
     5761 aacgaagagt cacacttgag gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa
     5821 aacgcaagat cttgactttg aaaatcacag attatacttc atcatttatc gtcaaaaaat
     5881 tctcgaacgg cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt agctggttaa
     5941 aggtacgagg aagtattcaa gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag
     6001 atatcataga agtcaaacat acacctcgca aagattatgc tcctgaaggg gaaaaacgag
     6061 tagagcttca tgttcatagc aatatgagta cgatggatgc aacaaacagc atctctgatc
     6121 ttgtggcaca agcaggaaaa tggggacacc gagctatcgc cattacagac catggtggtg
     6181 cgcaagcctt tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg
     6241 gcgtggaagc gaatgtcgta gatgatggtg tcccaattgc ttataatgat gcacatgaag
     6301 cattgagtga agccacatac gtcgtatttg acgtggaaac gacaggattg tctgcagtat
     6361 atgacacgat catcgagcta gccgcagtca aaatgtataa aggaaatgtg atcgaaagct
     6421 ttgatgaatt tatcgatcca ggacatccat tgtcacgaac aacggtagat ctgactggta
     6481 tcacggatga aatggttcga gggtcaaagt cggaagaaga agtattgcgg atgttccttg
     6541 aattttcaaa agatacaatt ttggtagccc acaacgcggc ttttgatatg gggttcttga
     6601 atactagcta cgcaaggtat ggtattcctg aagcggccaa tccagtcatt gatacgttgg
     6661 aattagctag atatctgtat cctcaattca aacgtttcgg attaggtgtg ttatctaaaa
     6721 agtttggtgt tagcttggaa cagcatcacc gagcaatcta tgatgctgaa gcaactggtc
     6781 acttagcatg gatctttgtg aaagaggcaa tggacaatca taacatgctt tatcatgatc
     6841 agctaaatga gcatatcgga gaaggagatt cttacaaaag ggctcgacct ttccatgtaa
     6901 cgattttagc aaaaaatcag gcaggactga aagatttgtt caaactgatt tcaatgtcga
     6961 atgtcgaata cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg
     7021 aaaatctatt gatcggctca gcttgtgata agggagaaat attcgaagcc atgatgcaaa
     7081 aaggggtaga agaggcaaga aaccgagcaa aattctatga ttatattgaa gttatgccaa
     7141 aagcggtcta tgcgcctttg atcgagcaag aattggtaaa aaatgaacat gatttggaag
     7201 aaattatcca aaatttggta gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag
     7261 gtaatgtaca ttatctgaac gaagaagatg ccatttaccg aaaaattttg atcaattcta
     7321 tgggcggagc gaatccgttg aatcgtcata gtttgccgga tgtccatttc cgaacaaccg
     7381 atgagatgct aactgcattt cactttttag gggaagaaac agcgaaagag atcgtagtag
     7441 aaaatacgaa taaaatcgca gatatctgcg aagaagtgat tccagtcaaa gatgaacttt
     7501 atacaccgaa gattccaggt tcagaagatg aaatatcaga attgagttac acaaaagcaa
     7561 aacaaatgta cggagatccg ttacctgaga tcattcaaaa acgattagaa aaagagttga
     7621 attctattaa tggtaatggc ttctcggtta tttatctgat cgcccaaaaa ctagtgcata
     7681 aaagtaatga agatggctat ctagtaggtt cacgtggctc tgttggctct agctttgttg
     7741 cgacgatgac aggaatcact gaagttaatc cattagcccc tcattattat tgtccagagt
     7801 gccaatattc cgagtttttt gaagatggta cgtatggttc tggatttgac atgccagaaa
     7861 aacaatgtcc aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt ccatttgaga
     7921 catttttagg attccatgga gacaaagtgc ccgatattga tttgaacttc tcaggtgatt
     7981 atcaagcaga agcccataac tatacgaaag tattgtttgg cgaagactat gtttaccgtg
     8041 ccggaacgat tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa
     8101 gggacaataa tctgcagttt cgaagtgcag aagtcgacag attagcaaaa ggcgctactg
     8161 gagtcaaacg gacaaccggc cagcatccag gggggatcat cgttattccc gattatatgg
     8221 atgtctatga ttttacgccg atccaatatc cagcagacga tcaaaattca gaatggaaaa
     8281 cgactcattt tgatttccac tcgattcacg ataatgtatt gaaattagat attctagggc
     8341 atgatgaccc aactgttatt cggatgcttc aagatttatc agggatcgac ccacaaacga
     8401 tccctacgga tgacccggaa gtgatgcgga tatttgccgg tcctgaagta cttggagtaa
     8461 gtcaggaaca aatttattcc aaaacaggga cattaggtat tccagaattc ggcactcgtt
     8521 ttgttcgagg gatgttagaa gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt
     8581 ccggactatc tcatggtaca gacgtatggt taggaaatgc ggaagaattg attcgacgag
     8641 gagatgcgac actggcggaa gtaatcggct gtcgtgatga tattatggtt tatttgatcc
     8701 atgctggatt agatagcggg atggcattta aaatcatgga aactgttcgt aaaggacaat
     8761 ggaacaagat ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg
     8821 tgcccgactg gtatatcgat tcttgttcaa aaatcaaata tatgtttccg aaagcccatg
     8881 ctgcagctta cgtattgatg gctcttcgtg tcgcttattt caaggtttat ttccctattt
     8941 tgtattactg tgcatacttt tctgtacgtg cagacgactt tgatctagtc tccatgtgca
     9001 aaggaaaaga tgctgtgaaa caagcaatga aagaaatcac ggacaaggga ttagatgctt
     9061 ccgtaaaaga aaagaatcaa ctgacagtgc tggagttagc caatgaaatg ctggaacgag
     9121 gattcaaatt tggaatgatt gatttgtata aatcggatgc cgttaacttt gttattgaag
     9181 gtgatacgtt gatcgcacca tttagagctg tcccaagttt gggaaccaac gtggcgaaac
     9241 aaatcgttga agcaagaaaa gacggtccat ttttatccaa agaagatctt gccacccgcg
     9301 gtaaagtttc aaaaacattg atcgaatata tgaacgacaa tggcgtattg aaagatctac
     9361 cagatgaaaa tcaattatcg ttattcgata tgctataagc atctgttttc ctgtctgctt
     9421 gtaattttaa gcagagtatg gtatagttct tataggtaat gatactaact gtgagtgagc
     9481 ggatttttcg ctcactcttt ttaatggaat tacgcactta atttttaagg aggcgaaact
     9541 tttgagtagc gtcgttgaaa cagtcacaga aatggtgaca ccgattttag atgaacaaaa
     9601 ttttgaactt gtcgaagtcg aatttgtaaa agaaggaaaa agctggttct tacgagtatt
     9661 tattgacaaa gaaggcggta tcgatatcga agaatgcgcc tttgtcagtg agaaactgag
     9721 tgaaaaactt gatgcagcag atccagatcc gattcctcaa gcttatttcc tggaggtatc
     9781 ctccccggga gcagagcggc cattgaaaaa agaaagcgac tatgaaaagg cacttggtga
     9841 ttatgtccat gtctccttat accaaccagt agatggcgaa aaacaatatg aaggatttct
     9901 tcaatcattt gatgctgaac agcttacatt gaaaatacgc ataaaaacac gagaaaaaga
     9961 attgactttt gaccgaaaaa atattgcaaa agctcgctta gctatccaat tttaatcacg
    10021 gagaggaatc agaaaaaaat gagtaaagaa atgttaaatg cgttagacgc tttagaagct
    10081 gaaaaaggga tctcaaaaga aatcgtgatc gatgctttag aagcagcatt ggtttctgca
    10141 tacaaacgtc actatggcca agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga
    10201 aagatccatg tctatgcggt aaaagaagta acggaagaag tgatggattc acagttggaa
    10261 gtctcattga aagatgcact tttaatcaat ccagcatatg agatcggtga tacgatccgt
    10321 tttgaagtaa caccaaaaga ttttggacgg atcgctgcgc aaacggctaa acaagttatt
    10381 ttacagcgtg tacgtgaagc agaaagaacg attatttata acgaattcag tgcttatgaa
    10441 aaagacatca tgcaaggaat cgtcgaacgc caggataaac gttatattta tgtcaatctt
    10501 ggtaaaattg aggcagttct ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca
    10561 catgatcgaa tcaaagttta tgtttcccga gtagaaaata cgtctaaagg tccgcaagta
    10621 tttgttagcc gcagccatcc agacctattg cgtcgcttat ttgaacaaga agtaccagaa
    10681 gtctatgatg gaatcgttga aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa
    10741 gtggcagttc gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag
    10801 ggacaacgag ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa
    10861 tggaatgaag atccagctgt attcatcgca aatgcattga atcctgcaca agtcatggat
    10921 gtgatcttcg atgagacaaa tccaaaggcc tgcacagttg tcgttccaga ctaccaatta
    10981 tcgctagcaa tcggtaaacg cggacaaaat gcacgtttag ctgctaaatt gactaaccat
    11041 aaaatcgata tcaaatcaga atctgacatg acggaatttt atgaaaatca agaacaacaa
    11101 aaagaaacag aagaactgca tgatgaagca atcgttcagt cagatttaac agacgatgaa
    11161 tatgaaacaa ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc ttaactttag
    11221 taaggaggca aagagatgaa acaaaggaaa attcctttac gcaaatccgt ggtttcaggt
    11281 gaaatgaaac ctaaaaaaga gatggtccga atcacccgtt caaaagaagg ggaagtatcg
    11341 atcgatccaa ctggaaaaat gcctggacga ggagcgtatg tctcacttga gccggaagaa
    11401 gtacaacaag cttgggataa aaaaattctc gatcgagtac tcgaaacaac attaacagat
    11461 gagttttatc aagaacttct tgactatgtc actcaccaaa aagcacgtaa agagttattt
    11521 ggaaaatgaa tacagttaat cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg
    11581 cgggaaaact aatcacgggt gaagaactga ccattgctga aatccgcaaa caaaaagcaa
    11641 aattggtgtt tgtggcaacc gatgcaagtg agaatacaag aaaaaagata aaagataaga
    11701 gttcatatta tgaagttcct tgctttgagc tattttctga agcagaaatc acgcagatga
    11761 tcggaaaacc tcgcaaggta ttcggaataa tggatgccgg atttgcgaag aagaccaagg
    11821 agctaattga aggttaggaa ggtgattgca tgggcaacaa aagaatctat gaattagcaa
    11881 aagaactcaa aaaatctagt aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg
    11941 tgaaaaacca tatgggtgcg attactagca gtgatgagaa gaaattgcaa caagcgttca
    12001 aaaacccaag cacgaaacaa caagctgcac aacaagcaag cagaaaacca gccaatcaac
    12061 agccgaacga tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc
    12121 gcagttatca agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa
    12181 caaaccaaaa taatacggga aataccagca gccaaaatcg tcaaggaacg agccaaggcg
    12241 gcaatcaaaa ccgtactaac aataacaaca aaccaaacaa taacaatcgc ggcaaattta
    12301 ataataataa ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag caacaatcaa
    12361 ctaaaccagc tgtaccacca cgcaaattcc gtgaattgcc agatgtatta gagtatactg
    12421 aaggtatgaa cgtagcagat attgcgaaaa aaattcatcg tgagccagca gaaatcatta
    12481 aaaaattatt tatgcttggt gtcatggtca atcaaaacca agcactggat aaagatacga
    12541 ttgaactgtt agcgacggac tatggcatgg aaccacaaga aaaagtccaa gttgacattg
    12601 ctgatatcga taaattcttc gaggcagaag aagtcgatcc agataaatta gtctcacgtc
    12661 cgccagtagt aacgattatg ggacacgtag accacgggaa aacaacctta ttggatacat
    12721 tgcgccattc acgtgtaaca agcggcgaag ccggcggaat cacacagcat atcggtgcct
    12781 accagatcga tattgatggt aaaccaatca ctttcttgga tacaccagga catgcggcct
    12841 ttacaagtat gcgtgcacgt ggagcaagta tcacagatat cacgatctta gttgttgctg
    12901 cagatgatgg cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg
    12961 ttccaatcat cgtcgctgta aataaaattg ataaaccagg tgcaaatcct cagcatgtta
    13021 tgcaagaatt aagtgagtat gaattaattc ctgaagcatg gggtggagaa acgatttttg
    13081 ttgagatctc agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg atcctgttag
    13141 tcgcagaagt agaagattta aaagcagatc caacacaacg cgcaatcgga acagtgatcg
    13201 aagcacgtct tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac
    13261 ttcacgttgg tgacccaatc gttgtcggta atacttacgg acgtgtccgt gtaatgacaa
    13321 acgatcttgg ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa atcactggct
    13381 tgaatgatgt acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac
    13441 gtgcagcggg tgaagaacgc gcaaaacgtg cattattgga aaaccgtgct gccacaagcc
    13501 gtgtgacttt ggacaactta tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg
    13561 taatcatcaa agcagatgta caaggttcag cagaagcact ggctgcatca ttgaagaaga
    13621 ttgatgtgga aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa
    13681 gtgatgtaac cttagcagca gccagcaacg ctattatcat cggtttcaac gttcgtccta
    13741 caccgcaggc aaaagtgcaa gctgatactg aagaagtcga tattcgtctt caccgtatca
    13801 tctataaagc catcgaagaa atcgaaactg cgatgaaagg gatgctagac cctgaatttg
    13861 aagaaaaaat caccggtcaa atgatcgttc gtgaaacctt caaagtttct aaagttggta
    13921 cgattgcagg tgcctatgta acagaaggat atattcgtag agatagcggt gtccgtgtaa
    13981 tccgtgacgg aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg
    14041 acgtgaaaga agtcaaaatg ggttatgaat gtggtgcgat gattgaaaaa ttcaatgaca
    14101 tcaaagtaga tgacgtaatt gaaggattta tcatggaaga agtcaaaaat gactaaaaac
    14161 tgataaaagg aggccaatca ttatggctaa ctaccgtgat cgccgagtcg gtcaagaaat
    14221 cttaaaagaa gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt
    14281 aacgatcaca gatgtgaaag ttacggggga cttacagcaa gcaacgatct attacagcat
    14341 tttatctgac ttagcatcag ataagaaaaa agcacaagat ggattgaata aagcatcagg
    14401 attgattcgt cgagagttag ggcacaatct aagtatttat aagacacctg aattgttctt
    14461 tgaacttgat gaatcagttg tctatgggaa ccatatcgat gaattgcttc gcgacttgaa
    14521 caaagactaa tcaaaaaatc ccgcaaatcc tgtgaaatca ggggttgcgg gatttttttg
    14581 ttttcattaa gacgtattac ctaagagttc catgacttta gtctggtagc gtgtcagcta
    14641 accaaaaaag agaaaaagaa aagtaaaaaa ttcgactttc ttttttctat agtatataaa
    14701 tttttgcata tagataaaga tcaagatacc gataacggaa gctagattgt aatctatttt
    14761 agtgacagtc aggtatagcc aagtaactag aagatcagtg gaacctgcac cataaaagtt
    14821 tgagtttgtc ggtccgttga cagttaaaag aaaaatcacg ttgaagttat tgatatttcc
    14881 gattacgccg cttcgatttg gtcacgatcc aaattttgga taatccctgt agaaattagc
    14941 atagtagctg ggataccaat tcacatattg acaaagataa cagagacttt tacataaaac
    15001 ggatcaatca ggaaaggaat tgctcgatcc agccccagtt ttccaacaat gtgttaactg
    15061 gcctagcgtc attcagcaaa ttatgcatga tgaggagcga aatgaactgc ggaacagcca
    15121 ttgtgataac gacaagtttt ttggatcaat gtaaaaaacg tcagcgaaag gaaaaatttc
    15181 gtggtttttt atacctgttc atttggctag atataaaatc caaacaaatg aacagaagtg
    15241 cttattaggt ttcaattggg agtatctgcg ctagaataag agtaataatg aaaaggagtg
    15301 ttatgtatgg attattctag taaagaatac tttgataaaa tgacggcttg gtggcgtgct
    15361 gctaattatc tttctgttgg gcaactttat cttaaagata accccttact gagacgtaca
    15421 ctgaaaccag aagatgtcaa aaaacatcca atcggtcatt ggggaacgat tcctggccaa
    15481 aattttattt atgtccattt gaaccgagtc atcaataaat atgatttgaa catgttttat
    15541 atcgaagggc caggacacgg cggacaagtc atggtatcga atgcttattt agatggaagt
    15601 tacacagaga tctatccaga agtaacggaa gatgagacag gaatgcaaaa attattcaag
    15661 cgattttcat ttcctggagg cattgcttct catgcagcac cagaaacacc aggttctatc
    15721 catgaaggcg gagagcttgg atactctctt tcacatgcag tcggagctgt attagacaat
    15781 cctgaagtaa tctctgctgt cgtgattggt gatggtgaag cagaaaccgg tcctttagct
    15841 ggaagctggt tctcaaatgt ctttatcaat ccagtgatag atggagctgt tttaccgatc
    15901 cttcatttaa atggtgcaaa aatagccaac ccaacgatct tggcacgtaa atcagatgga
    15961 gaattagcaa attactttaa cggacttggc tgggagcctt tctttattga aggaaatgat
    16021 ccagaaaaat taaatcctgt tatggctgaa aaaatggatc aagcaatcga gaaaatcaaa
    16081 tccatccaaa aagaagctcg cctgaagaca gcaacagatg tcgtgatgcc aaaatggcca
    16141 gtattgatcg tacgcacacc aaaaggatgg accggtccaa aagaatggga cggggaacca
    16201 attgaaggaa ccttccgagc acaccaagta ccgattcctg tagatcaaga acatatggat
    16261 catgcagatg ctttactccg ttggctgaaa tcctatgaac cagaaaaatt atttgatgca
    16321 caaggacgta ttttagaaga aatcagagaa attgcgccta ctggtgacca aagaatggca
    16381 aaaaatccta tcaccaatgg tggaatcgat ccaagaccgc ttattatgcc agattggaaa
    16441 aaatatactt tgcaatttga aaaaccaggc tccgttaaag cagaagatat gaccgaactt
    16501 ggtaaattcg ttcgcgagat cattgagaaa aatccagaaa atttccgtat atttggacca
    16561 gatgaaacaa aatcaaaccg attgaatcaa gttttcaaaa cgacaaatcg ccaatggatg
    16621 gaaaaaatcg aaccagaaaa tgatgaatgg ctttcgccat ctggtcgcgt catcgacagt
    16681 caattatcgg aacaccaaga tgaagggttc ttagaagggt acgttctgac tggacggcat
    16741 ggtttttttg caagttacga atcattcttg cgagtggttg actctatgtt gactcagcac
    16801 ttcaaatgga tacggaaatc acatgacctt tcttggcgaa aagattatcc ttcgctgaat
    16861 ctcattgcaa gctcaacagt cttccagcaa gatcacaatg gatatagcca ccaagatcca
    16921 ggaatattga cacatttggc agaaaagaag gcggagttca tccgtgaata tctgcctgcg
    16981 gatgcaaata cattgctcgc agtcatggat aaagcattcc gctcgtcaga aaaaatcaat
    17041 ttgatcatct cttccaaaca tccgcgtgct caattttact cagcagaaga agcagccgtc
    17101 ttagtgaatg aaggcttgaa aatcattgat tgggcttcga cagcaaaaga ggaggaacct
    17161 gaattggtca ttgctgctgc aggaacggaa tctaacttag aagcattagc agctgtaact
    17221 ttgctgctgg aagaattccc aaaactaaaa attcggttca tcaatgtcgt agatttattg
    17281 aaattacgtc atccaagtca agatccgcgt gggttgagcg atgaagaatt tgatcaatat
    17341 tttacaaaag acaaaccgat cttatttgct ttccacggtt atgaaacatt ggtccgcacg
    17401 attttctttg atcgtcataa ccatcattta atgattcatg gctacaaaga aaatggagat
    17461 atcacgacac catttgacat gcgtgtagta aatgaacttg atcgttatca tttggcaaaa
    17521 gacgcagctt taaaaatcaa aggaagtcaa gcagaagatt ttgcggaaaa aatggatcag
    17581 aaattacaag aacatcaaaa ctatattcgt gaaaatggta ttgatctgcc agaagtattg
    17641 gattggaaat ggaaaaatct tgaccaataa aatgataaaa tcgatcccgc aaaacagaat
    17701 tttttacatt gtattatttg tcaaattaag
//
LOCUS       DAZAID010000059        17704 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000059 DAZAID010000000
VERSION     DAZAID010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.059"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..409
                     /locus_tag="ACG9BE_001851"
     CDS             179..409
                     /locus_tag="ACG9BE_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFN3032592.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            506..1807
                     /gene="murA"
                     /locus_tag="ACG9BE_001852"
     CDS             506..1807
                     /gene="murA"
                     /locus_tag="ACG9BE_001852"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFN3032593.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1820..1996
                     /locus_tag="ACG9BE_001853"
     CDS             1820..1996
                     /locus_tag="ACG9BE_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFN3032594.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2131..2427
                     /gene="yidD"
                     /locus_tag="ACG9BE_001854"
     CDS             2131..2427
                     /gene="yidD"
                     /locus_tag="ACG9BE_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFN3032595.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2612..3079
                     /locus_tag="ACG9BE_001855"
     CDS             2612..3079
                     /locus_tag="ACG9BE_001855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3032596.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3164..4048
                     /locus_tag="ACG9BE_001856"
     CDS             3164..4048
                     /locus_tag="ACG9BE_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN3032597.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4162..4398
                     /locus_tag="ACG9BE_001857"
     CDS             4162..4398
                     /locus_tag="ACG9BE_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFN3032598.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4486..4566
                     /locus_tag="ACG9BE_001858"
     tRNA            4486..4566
                     /locus_tag="ACG9BE_001858"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4520..4522,aa:Tyr,seq:gta)
     gene            4573..4644
                     /locus_tag="ACG9BE_001859"
     tRNA            4573..4644
                     /locus_tag="ACG9BE_001859"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4605..4607,aa:Gln,seq:ttg)
     gene            4769..4939
                     /locus_tag="ACG9BE_001860"
     CDS             4769..4939
                     /locus_tag="ACG9BE_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032599.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5159..5866
                     /locus_tag="ACG9BE_001861"
     CDS             5159..5866
                     /locus_tag="ACG9BE_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3032600.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5925..6569)
                     /locus_tag="ACG9BE_001862"
     CDS             complement(5925..6569)
                     /locus_tag="ACG9BE_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFN3032601.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6695..6982
                     /locus_tag="ACG9BE_001863"
     CDS             6695..6982
                     /locus_tag="ACG9BE_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032602.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7090..7410
                     /locus_tag="ACG9BE_001864"
     CDS             7090..7410
                     /locus_tag="ACG9BE_001864"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3032603.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7491..8804
                     /locus_tag="ACG9BE_001865"
     CDS             7491..8804
                     /locus_tag="ACG9BE_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFN3032604.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8801..9520
                     /locus_tag="ACG9BE_001866"
     CDS             8801..9520
                     /locus_tag="ACG9BE_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFN3032605.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9648..10199
                     /gene="raiA"
                     /locus_tag="ACG9BE_001867"
     CDS             9648..10199
                     /gene="raiA"
                     /locus_tag="ACG9BE_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFN3032606.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10421..12955
                     /gene="secA"
                     /locus_tag="ACG9BE_001868"
     CDS             10421..12955
                     /gene="secA"
                     /locus_tag="ACG9BE_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFN3032607.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13081..14182
                     /gene="prfB"
                     /locus_tag="ACG9BE_001869"
     CDS             join(13081..13152,13154..14182)
                     /gene="prfB"
                     /locus_tag="ACG9BE_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFN3032608.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14287..14973
                     /gene="ftsE"
                     /locus_tag="ACG9BE_001870"
     CDS             14287..14973
                     /gene="ftsE"
                     /locus_tag="ACG9BE_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFN3032609.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14966..15850
                     /gene="ftsX"
                     /locus_tag="ACG9BE_001871"
     CDS             14966..15850
                     /gene="ftsX"
                     /locus_tag="ACG9BE_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFN3032610.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16002..17450
                     /gene="cls"
                     /locus_tag="ACG9BE_001872"
     CDS             16002..17450
                     /gene="cls"
                     /locus_tag="ACG9BE_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFN3032611.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         5920 a         2984 c         3838 g         4962 t
ORIGIN      
        1 agggactgcg acaagacttt tgtctcagtc cctttttttg catattttta gcatttattt
       61 cctaaaaaat gtaaggggat tcctatattt ttaagtatac ttgtttttcc ttttatacat
      121 tttatggtat tatcatttta ttagttatta taaaacaatc gttagaaagg acgtcattat
      181 gcaagtatac ggaattgatg cggtgcttcg aatcgtgagt catttggcgt ttatctactt
      241 agccttctgg tctttacgtt cgttacgcat tgaaactttt ttcagaccaa tgcataatac
      301 gcaaattaga atggcaatcg tacttttttc aattttttta gggtacactg caagtaattt
      361 tttcttagaa gttatcgctt tatgccgaaa tcttttcgtt agcctgtaaa ttttaaaaaa
      421 aggtactatt ttaaaagcaa ggatgttata atagaaaaga ttttgagtaa gtgtatgctc
      481 attggaattt tttggaggga acaacatgga agagatcatc gtaagaggtg gcaatcaatt
      541 aaatggaaca gtaagaattg aaggagctaa aaatgctgtt ttgcctattt tagctgctag
      601 tttgttagca gaagaaggta ttacgacatt agacaacgtt ccgattcttt ctgatgtctt
      661 cactatgaat caagtgattc gtcacttaaa cgtagatgta gattttgatg aacaaaagaa
      721 tcaagtaaca atcgacgctt caagacaatt agaaatcgaa gcgccttatg agtatgttag
      781 tcaaatgcgt gcttcaatcg ttgtgatggg tccgctactt gccagaaatg gacatgcaaa
      841 agtagctatg cctggtggat gtgcgattgg taaacgacca atcgatttgc atttaaaagg
      901 atttcaagct ttaggagcaa aaattattca aaaaaacggc tatatcgaag caattgcgga
      961 tgaattgatc ggtaacacga tttatttaga tttccctagt gtaggtgcta ctcaaaatat
     1021 catgatggct gccgtaaaag caaaaggaac aaccatcatt gaaaacgtag ctcgcgaacc
     1081 agaaatcgtt gatttagcaa acattttgaa taaaatgggc gctcaagtct atggagctgg
     1141 tactgaaaca atgcgtattg aaggcgtgga tcatttacat gcagtcaacc actcaatcgt
     1201 acaagatcgt atcgaagcag gtacctttat ggttgctgct gcaatgacac aaggcaatgt
     1261 gctgattgca gatgctatct ctgaacataa ccgcccattg atttctaaat tgatcgaaat
     1321 gggtgcagaa atcattgaag aagaaggcgg agtccgtgtc atcggaccaa aacatatctt
     1381 acctacagat gtaaaaacga tgcctcatcc tggtttccct acagatatgc aagctcaaat
     1441 gacagctatc caattagttg cagaaggtac aagtgtagtg actgaaacag tgtttgaaaa
     1501 tcgtttccaa catttagaag agatgcgccg tatgaacgct catgtcaaaa tcgacggaaa
     1561 tgtagcaatc atggatggaa accatgaatt gcaaggtgca gaagtatatg caacagactt
     1621 gcgggcagca gcagcattag ttttagctgg cttgaaagca aatggaatca cacgtgtgcg
     1681 taacttgaat tatctagacc gtggatatta taatttccat atcaaactac agcaattagg
     1741 tgcagacgtt gaacgtgtag acatggatca aacttctgct gaaaaaacag cacaaacgat
     1801 tgcttaagga agtggctgaa tgagtaacca acgatatatc ttattaactt tgataaagat
     1861 tttaatggtg atcttattgc ttatccttct ttttgtggcc ggaacgatga tcggatatgg
     1921 tgtcatcggt ggcggcaatc catttaaagt atttcagcca agtttgtgga ttcatattcg
     1981 agactttttt cattgataaa taagaatcac agtggttcta gtcgatatga agtgagccat
     2041 gaagcagaac atgataaaag aaaacagaag ggaacgttga cgttagtcgc gatccctttt
     2101 tttagtaaaa gaaaagaagg aatctaattg atgaagaaga tattgattta tttagttaga
     2161 ggatatcagc gctttatttc gcctgcattt ccacctagct gccgttatta cccaacctgt
     2221 tccaattata tgataaaagc aattcaaacc catggcgcgc taaaagggac ggtaatgggg
     2281 actgcacgga tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta tgtaccattg
     2341 acattccgat taacgcgaaa taagaaagat aaaaaagaac cagattataa aaaggttgtg
     2401 aaaaatacgg gtagcactga gcgataaaga aacaatttgg aaaatagctt cttatatttt
     2461 gcaaaattgt gagttattgc cgaagtgttg gattttgaac accgtcaaaa aaaaggttgt
     2521 gaaaaatacg gctagcactg agcgataaag aaaaagagga aatttattgt caaagtaaca
     2581 actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca agaaagattt
     2641 accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag tatatgtttc
     2701 cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac tgatctatgt
     2761 ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta accccaacgt atcaaggaaa
     2821 ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacaac tcgcttccag
     2881 tcaaactcag atcatcatac gaaccggcag tactagcagt gcggcgttac atctctatca
     2941 aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc attactctga
     3001 gcccattttt gaaaatggag atcaattaag ggatcaagtg attttagcaa ttcacttacc
     3061 taaaaatagc tttaactaat aaaatttcaa ttcaagaaaa tgctttcaaa tgctaaatag
     3121 atacattaca atagctttag caaacaaaaa ggagaatatt tttatgggaa tattagtttt
     3181 tgattttggt ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga ttaaaggtaa
     3241 gggacaattt gcaacgcctg aatcttggga agaaatgaaa gcgcaattat ttcaagttta
     3301 taaaaaaata ctcgtttctt ttgaagtcga aggaattgga atcagctctc ctggagtagt
     3361 gaataatgag aaacaggtga ttgagggaat cagtgcgatt ccttatattc atggatttaa
     3421 tattttccga gatttggaaa ctctgtttca acttccagta acgattgaaa atgacgccaa
     3481 ctgtgccgga atggctgaat tttatgaagg tgctgcccgc gattatcaag atgttgcttt
     3541 tgttgtggca ggaactggga ttggcggggc gttgttcacg aaaggacaaa tcaataaagg
     3601 ggcacatctt tacggtggag aatttgggtt gatgttctta aatggcgata agacattcag
     3661 taaacttggt acagcggtac agatggcttg gcgttattgt gaacgcaaag gtctcgataa
     3721 aaatgcttat accggaaaag atgtctttga attagcagaa actggagatg caatagcaaa
     3781 agaagaagtt gagagttttt atacgtatct tacaaaggga ttattcagta ttcaattttc
     3841 gtttgatcca gaagtgatcg tattaggcgg cggggtttct gcaaaagaag ggctcattga
     3901 tgagatcaaa agccgaatga agaagctgac agaacaattc gatctgcatg atttcgaacc
     3961 gcagatttta ttatgtgaat acagaaacga tgctaatctt gtaggtgcag cagcaaacta
     4021 tctcgctgta cgtcaagggg agaattaaga gctttagaga aggtgcagct tgcataaaag
     4081 aagtgaatga aatgaatagg agatttttac ctaaggaatg ttttatttgc tgcagtatga
     4141 taaaatatct gagaggtgaa aatgatgtca aaaaaaaata aagatattga agtgcgtata
     4201 gaagaagtga aaaaagatat caaaggtaag aattatgaag tcactcagtt atttataggg
     4261 aaaaaaatga ttggtgagat cttagcctat ggaccaaaag aatatgaaat tttctttggt
     4321 gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt gattcgacac
     4381 tggaatctgc acgaataaaa aaatatcaaa aaaactttca ataaagggtt gcaaaaatct
     4441 ggaaaattag gtattataac taagtcaggt tgatacaaac ctttcggagg agtagcgaag
     4501 tggctaaacg cgacggactg taaatccgtt ccttagggtt cagtggttcg aatccactct
     4561 cctccatttc attggggtat agccaagcgg taaggcaagg gactttgact ccctcatgcg
     4621 ttggttcgaa tccagctacc ccagtatacc acaatcgtaa gctctcgtta gttcgagagc
     4681 tctttttttt gctttgctac cttgttcctt gaagattctg ctgatttaag atagagttaa
     4741 gttaactaca agaattggag ggatccacat gaaatggaaa gaaaaagtag acgaagcagc
     4801 tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat attgaagtga atattccgaa
     4861 aaaaggcgga aaagcggtcc gggtaaaatc aaaacgccca acgaatcata caaaaaaatg
     4921 gatggctaaa aaccgctaat aataacaaaa gatccataag tcggcatttt catcgatttt
     4981 agggtctttt gttattttct aggttggaca tatctaaaaa attgtttctg acaataggag
     5041 taggaagaac gatgtgtgtc taaatcctct gttgatggat agaaaccttt atctttttca
     5101 gttgtgaatc aggtatatac cagttataat agaagaaacg aaaggacggg tgtacgttat
     5161 gtcgaatcaa ttacctgtat atatacagat tcatgaccaa ttaaaaaatg aaatcgaaca
     5221 aggtatatgg aaagtcggag accgtcttcc ttcagaaaga gaattagcag tgaaattcaa
     5281 tgtcagccgt atgacgttga gacaagcaat tcaaaacttg gcagatgaag gaatcttaga
     5341 aagaaagatc ggttcgggta catatgttgc acgagaaaaa gtacaagaaa aaatgtctgg
     5401 tgcaacaagt tttacagaaa ttatggaatc acaaaatcga gtaccgtcta gtcggacaat
     5461 ctcttatttc ttgacttcgc caagctcaag tgaaatggaa aaactaaact tgagtaaaga
     5521 tgacacgatc ttgcgaatgg aacgtattcg tttcgcagat gatgtcccta tttgttttga
     5581 agttgccagt gtgccacaaa agctgatcga tggctacagc aaagcagaga ttactgaatc
     5641 gttgtatcgc acgctagaag aaaaaggcgg gaaaaagatc ggtccagcga atcaaacgat
     5701 tactgctgtg ttggcttctg agaaaattgc tgactattta gaggtaaaac gaggagatgc
     5761 agttcttcgt ttgcgccaga tttcttattt tgaagacggt acgccgtttg agtatgtgcg
     5821 tacgcaatac gtaggaaatc gttttgagtt ttatttggaa aaataaaaaa caggtgatcc
     5881 ctgattccgt attagaaata aaagagaatc agaatcacct gtttttatat atgatcagtt
     5941 accagttgct cgtgataaga aacaggaccc tcttcaaatt ggacttgtcc gttcagcagc
     6001 tcaatgatgg attctttaaa gtggtcagca cttttttcat ctacagcaca gatgacgcta
     6061 acttgatcag tatattggat ttcttttata aaaaaatgtt ggagttcaag ataattctgt
     6121 aattttccta gttgcggata cgtgatcatc acagagatct cctgttgcaa ggtaccttct
     6181 acgattccaa tgtcagacaa tgcttgagag accgcatgag tgtatgctcg tattaatccg
     6241 cctgtgccga gtttcgttcc tccaaaatat cgagtgacta ctgcagctac attgatcagt
     6301 tgattttttt tgaggacttc caacatcggt atgcccgccg ttccacttgg ttctccatca
     6361 tcgctgcttc gctggatttc atttttttct ccaatgatga aggcgctgca gttatggttc
     6421 gctttccaat gttctttttt tatttcttga atgaaacttt ttgcttcgtc ctctgattct
     6481 attcgtctaa gagaacagat aaagcgtgat tttttgatct cgatctcact ttgtccattt
     6541 tcttttattg tacgatagcg ctcgatcatc ttgtcacctc ttttttcatt ttctttattc
     6601 agtataaata aagttctagg ttgtttcaac actgtaacgg ttgcgcaatt gctgtagtat
     6661 gcgataataa agacaaaggc aggtggatct ggggatgcag gaacgaatga aaaagtacga
     6721 tgcaattacg cattatttga agaacaatgg cggctctcaa gtaacgttga cctttaccca
     6781 atttgacgaa ctgctgtttc ccagtaacgg acttcccaaa acagcaaggg aaagtacaga
     6841 ttggtgggca aatgattata aacaccctga aaaaggtgcc tatggctgga taaatgcagg
     6901 ctatgaagta gttgtcatca atttggacaa agaatatgtc gtgttcaata aattagtgaa
     6961 atctagttgg ctatttgatt aaataaaccg cgtttagaaa acgttgacaa atttctttct
     7021 ttttatataa tagagtaaca gacgcgaaga aaacgtttgc gtagtttgtt ttaaaaagga
     7081 gagaaaagta tggctaaaaa aacaatcatg cttgtatgtt cggctggtat gagcacaagt
     7141 ttactagtaa caaaaatgca aaaagctgct gaagcaaaag gaatcgactc tgatattttc
     7201 gctgtgtccg cttctgacgc agataacaat cttgcaaata aagacgtgga tgtgttgtta
     7261 ttaggcccac aagttcgctt tatgaaagct gattttgaga aacgtctgga accaaaaggc
     7321 attcctttag atgtcatcaa tatggctgat tatggtatga tgaatggcga aaaagtgtta
     7381 gatcaagcaa tcacgctcat aggtgaataa atagaacagt ataactatcg aacaagcgat
     7441 ccaaaaaata gatcgcttgt tttttcgtat atttttttag ggcgaataat atgaatcatt
     7501 tttggggtag aaggcttctg acgagagata ttaaagcaat agactgtgag gaggtaaatt
     7561 taccaaatta tgagaagatc gcagcagtgg aacgtttgag caactggatt ctttgtcatc
     7621 gctgtggaat gagaacgtcc gtcattgatg gacagcttgc agattacggt tatttttgta
     7681 cacattgtct atatttaggg cgatgcgaca ctcaacaaga tttatatttg tttgatcagc
     7741 ctgaagcaaa acctcgagaa gtcgtctttt tttggacagg acagcttact gaaaaacaaa
     7801 cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt atttgggctg
     7861 taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact ttaaaagctg
     7921 gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg tttttacgct
     7981 atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca gcaacggctt
     8041 atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt tatcggtcct
     8101 ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat gcaggattag
     8161 ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta agtgccacac
     8221 ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa cttgctttac
     8281 gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat tggcgacaaa
     8341 aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa ttaattaaag
     8401 aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg aaagccacac
     8461 ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac aatcagcggg
     8521 cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc acgatgatcc
     8581 ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg gatcatccgg
     8641 tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa gggccttata
     8701 cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga aaagcttgtc
     8761 aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca gttgctgcag
     8821 taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga aatacagatt
     8881 acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa gaggctgttc
     8941 tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag taaattctat
     9001 ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct atgattttca
     9061 gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc aatacaagtt
     9121 tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga taaaaaaaga
     9181 atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa cagaaagagg
     9241 attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga tgctactcaa
     9301 aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat tggagatgcc
     9361 tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa tattgattat
     9421 tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt tgcagatcgc
     9481 acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg gaaatttgat
     9541 agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa gagggaaaga
     9601 gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg tttagatata
     9661 atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt gagaaaaaag
     9721 taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat gtgaacttaa
     9781 aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct tatcttgtat
     9841 tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt gttgacaaat
     9901 tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt gaaacgggca
     9961 tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa aatgattctg
    10021 aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt gaagaagcag
    10081 tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca gaaacaaatg
    10141 gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa acagactgat
    10201 cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc cgcaagtttt
    10261 tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct tactttaata
    10321 cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa gtctacatag
    10381 tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt tgattcgttc
    10441 acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa aaaaagttga
    10501 agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa aaactgctga
    10561 attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg aagcttttgc
    10621 agtcgtccga gaagcagcaa aacgtgtatt aggtttatat ccataccacg tacaattgat
    10681 gggtggaatc gtgcttcacg atggaaatat ccctgaaatg cgtactggtg aaggtaaaac
    10741 cttgaccgca acgatgccag tatatctaaa tgctctgact ggcgaagggg tacacgtagt
    10801 taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgaac tttataattt
    10861 cttaggatta acagtggggt tgaatatcaa ctcaaaatct tcggatgaaa aacgcgaggc
    10921 ttataattgt gatatcactt atagtacgaa taatgaatta ggatttgact acttgagaga
    10981 caatatggtc gtttaccgcc atcaaatggt acaacgtccg ctgaattatg ccattgtcga
    11041 tgaagttgac tcaatcttga ttgatgaagc gagaacgccg ctgatcattt cagggcaagc
    11101 agaaaaatcc acagctttat atacaagagc agacaacttc gtgaaacgtt tgaaagaaga
    11161 agaagattat aaaatcgatg tacaatcaaa aacaatcagt ttaaccgaag cagggatcga
    11221 aaaagcagaa gaaacattcg gtttggataa cttatacgac attgaaaata ctgcattgac
    11281 ccatcacttg gatcaagcat tgagagccaa ctatatcatg cttttagata tcgattatgt
    11341 tgtccaagac ggaaaagtac tgattgttga ccagttcact ggtcggatca tggatggacg
    11401 ccgttattca gatggacttc accaagcaat cgaagctaaa gaaggcgtag agatcgaaga
    11461 cgaaacgaaa accatggcga cgatcacttt ccagaactat ttccgtatgt acaagaaact
    11521 tgcgggaatg actggtacag ctaaaacaga agaagaagaa ttcagagaaa tttataacat
    11581 ccaagtatac cagattccga caaatcgtcc ggttatccgt gatgatcgtc cagatttgct
    11641 ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa gatatcaaag atcgctaccg
    11701 caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa acatctgagt tattatcaga
    11761 tatgctgaat caagaacgta ttcctcatga agtgctgaat gcgaaaaatc actttaaaga
    11821 agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg acgattgcta ccaacatggc
    11881 tggtcgggga acagatatca agttaggact tggggtaaga gaacttggtg gtctagcagt
    11941 tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag gacgtgcagg
    12001 acgtcaagga gaccctggta tgtcacagtt ctacttgtca ttggaagatg atttgatgaa
    12061 acgattcggt tcagaacgaa tcaaatcttt cttagatcgg atgaagatcg gtgaggaaga
    12121 tgctgttatc caaagcaaga tgctgacgaa acaagtagaa tctgcacaaa aacgtgtaga
    12181 aggaaataac tatgacacac gtaaaaacgt cttacaatac gatgatgtga tgcgtgaaca
    12241 acgtgaagta atctataaac agcgacaaga agtaatcatg gaagaaaaaa gcttatccaa
    12301 gccactaatg aacatggtca aacggacaat cagtcgtgta gtagatgcgc atacacaatt
    12361 agagaaagaa aattggaatt tggaaagtat catcgatttt gccggcagta ctcttgtcca
    12421 tgaagatagt attactcttg cagatatcga aggaaaaaca gctcaggaga tcaaagagta
    12481 tctaaacgaa cgagcacaag aagtatttaa taccaaagcg gctcaactaa atggtccaga
    12541 gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt gtggactcta aatggacaga
    12601 ccatattgat gcaatggatc aattacgtca gtcaatcggc ttacgtgctt atggacagaa
    12661 caatccatta gtcgaatatc aaacagaagg ctataagatg tttgaagata tggtaggcgc
    12721 catcgagtat gaagtcactc gaatctttat gaaagctgag atccgtcaaa acgtacagcg
    12781 tgaacaggtt tctcaaggaa cagcttctca ctcagaagac ggagatgcca aagaaaatag
    12841 taatactagc gcgaaaaaac agccaatcca tgtgaacaag gtcggaagaa atgatccttg
    12901 tccttgtgga agcgggaaaa aatataagaa ctgtcatgga aaaaatgcaa actaatttct
    12961 gacagcagga ttggtgagac aaattctttt tttaagaatt cgtctcacct tctttctgtt
    13021 atttgctgat tctgttagac tagatataat gaaaaaagta tagtgaaaga aggaaaaaac
    13081 atggaaatat ccgaaattcg aaatctcctg acagaaatgc aggaaaaggt cacaagcttc
    13141 aggaggtctc tttgacttag accagctgga agaagacatt gcggaagcag aagcaaaaat
    13201 ggctgaacct ggtttttggg atgatagcga agctgctcaa aaagtgatca gtgaaaacaa
    13261 tgcaaataaa gaaacttatg atcaattcca ttctcttgct gaagaactgg aagaaatcga
    13321 attgatggtg gagatgttgc aggaagaacc tgatgcagcc atgcaagaag aagcagaaac
    13381 acgtatcaat gcgctagatg aaaaaatgaa gacatatgaa ttatctatgt tgttagatgg
    13441 tccttatgat cgaaacaacg ccatcgttga attgcatcca ggggctggcg gaacggagtc
    13501 ccaagattgg ggcagtatgc ttttacgtat gtatacacgc tgggcagagc agcatgggta
    13561 tcaagtagag acactagatt atcaagcagg tgatgaagca ggaatcaaga gtgtgaccct
    13621 actgatcaaa ggacacaacg catttggtta tctgaaatca gaaaaaggtg ttcatcgcct
    13681 tgtacgtatc tcgccttttg actcagcgaa acgacgtcat acttcttttt gttcggtaga
    13741 tgtgatgcca gagcttgatg aaacgatcga tatagaaatc aatccagatg atttgaaaat
    13801 cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc aataaaacag aatctgctgt
    13861 acggatcact cacttgccta caggaacagt agtagctagt caggcacaac gttcacaatt
    13921 gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag ctttatcaat tagaagtgga
    13981 aaagaaagaa caagaagcag ctgcacttcg aggagaacaa aaagaaatcg gctggggttc
    14041 acagatccgt tcttatgttt tccatccata ttctatggta aaagaccatc ggacaaacgt
    14101 agagacagga aatgtccaag cagttatgga cggtgacctt gatttattta ttgacggcta
    14161 tttaaaaatg catctacaat aagaacccat tgttaaattg aaataaaatt gtaagatttt
    14221 gcaagcatgt tgaaacattg ccatgctata atagctaaag actgaaaaag atatggagag
    14281 aacgccatga ttgaaatgaa ggatgtaatg aagaagtact caaatggtac gaccgctatc
    14341 cgcaatctct caattgtcat cgaacaagga gagtttgtct atgtggttgg tccgtctgga
    14401 gccggtaaat ctacattcat caaattgatg tatcgagaag aaaaagcttc aaaaggaact
    14461 ttgaacgtcg caggtcatga tctaatagca atcaaaaacc gcgaagttcc ttatctacgc
    14521 agagaaatcg gcgtggtgtt ccaagactat aaattattac caagaaaaac agtatatgaa
    14581 aatgttgcgt acgctatgca agttatcggc agaaaacctc gggatatcaa aaaacgagta
    14641 atggaagtgc tggaccttgt tggtttgaaa cacaaagttc gagtctttcc tagcgaatta
    14701 tccggtgggg aacagcaacg tgtctctatt gctcgggcaa tagtgaatac gcctaaagta
    14761 cttatcgcgg atgaaccgac aggaaatctg gatccagaaa actcatggga aatcatgaaa
    14821 ttattagatc ggatcaatgc ccaaggaact actgtcgtga tggctacaca taacagtacg
    14881 atcgtaaata cgattcggca ccgagtaatt gctatcgaaa acggccgcat tattcgagat
    14941 caagcggaag gggaatatgg atacgatgat tagaaccttt ttccgccatt tattagaaag
    15001 tatcaagagt ttgaaacgta acggctggat gacagttgct tcagtcagtg cggtaacgat
    15061 cacattgact ttagtcggtg tattcatggc agttatcatg aacgcaacta aattagctca
    15121 agatatagaa ggaaacgttg atgtctctgt attcgtagat atcggtacaa aagacgaaga
    15181 gatgaaaaag ctagaaaaag aattgaatga cttagatcat gttaaaaaag ttgaattttc
    15241 aagcaaagaa caggaattga aaaaaatcca aaacgaaatg ggtgattcct ggaaattgtt
    15301 tgaaggtgac aataacccgt tatatgatgt gtatgtagta agtgctactg atccttctta
    15361 tacaaaacaa gtagcagaag aagcagcaga tttgaagaat gtttctcgtt ctgattacgg
    15421 tggagcttcc tcagatcgga ttttccaaat cgctggtgca gttcggacat ggggattagt
    15481 tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt tcgaacacga ttcggatcac
    15541 gattttgtct agacagcgtg aaattcaaat catgcgttta gtcggtgcaa aaaatggata
    15601 tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt ctgatcggtg cgattcttcc
    15661 gattttgatc atcacatttg gttatgcatg gtttttccaa ttgatcaatc catcattatt
    15721 acgttcccat tattcattga ttcatccaca aaatatcgtt tggaaaatca atcttttaat
    15781 ggtcggcatt ggtgtgatca ttggttcact tggctcgatc atttctatga gacgtttctt
    15841 gaaattctaa taccacaata taaataaaat gagaaagttg gacagtttcg ttccagcttt
    15901 ctttttttat tggctaggag aatagaagga agcggctttt ttccctttac tttaaaaaac
    15961 aacttgggta ttcttaagtt ggtagaagaa gaggtgaagc cgtgaaaatt atcgttgaca
    16021 atttttttac gatccttttg attatgaata ttttgctttc ctttatcatc gtttttcgag
    16081 aaagaaagga aactgctcaa acatgggcat gggtgctcgt tttgatgttc attccagttg
    16141 taggatttat tttatatatt tttttaggaa gagggatttc caaagagaaa atctttgatt
    16201 tgaagatcca agggaaaatc ggtaagaacc tggaaattga agaagacaaa caggcattga
    16261 tgcgagggct ttatcctcat ccgcctactg gacaagtaga tgtgaaacaa ttgatctata
    16321 tgctcacggt atttgaaagt acgctctata cgactaaaaa tgaaatcact ttatttacag
    16381 atggtcgtga aaaattcgat gcgctgattg aagatatcaa gcaggcagaa gaccatatcc
    16441 acttccagta ttatatttat cgcagtgatg cattaggtga agaagttcga gatgcattga
    16501 cagaggcggc aagacgaggg gtaaaagtca gagtccttct tgatgcatgg ggttccacac
    16561 aagtttcttc cagctttttt cagaatttga aaaaagcagg cggagaagtc gcatttttct
    16621 ttcctctatt tgttccgtat attaatcctc ggatcaatta tcgcaatcat cgtaaaatcg
    16681 tagttattga tgggaaaatc ggatatacag gtgggttcaa tgttggaaat gaatatctag
    16741 gattggtaaa gaaatttggt tattggcgag acaatcattt gcgtatttac ggagaagcgg
    16801 tgtatacctt gcaaaaccgt tttttgatgg actggaattc tcagcataca aaagaagtag
    16861 gctattctcc caaatttttt ccgtccattc actctactgg tgatatagct gttcaaatcg
    16921 tgacaagcgg accagatacc gaacatgaac aaatcaaaat gacttatttg aaaatgattt
    16981 cgttagccaa acgggagata ttgatccaaa cgccttatta cataccggat ggatcgattc
    17041 atgaagcact gaagttggct cttctttcag gcgtaaaagt tcatatccaa atacctaaca
    17101 aaccagatca tcttctcgtt tactgggcaa cgtattcttt cgctgcagag ctaatcgagt
    17161 atggagcaag gatcgaaaca tatgagaatg gatttattca tgcaaagacg atgattatcg
    17221 atggtggcat tgtttcagtt ggctcagcaa atatcgatgt tcgttcattt cgcttggact
    17281 ttgaggtcaa tacactggtt tatgatgaac gaatggcttc acgtgtaaga aaagcttttt
    17341 ttgaagactc gaaaatttct acgcatctca caaaagaaat gtatgaaaac cgaggaataa
    17401 tcatcaagat gaaagaagga ttagcaagat tgatttcgcc gctcctttaa aacataaaaa
    17461 ggacatgaga accatcaaca gatggagatc tcgtgtcctt ttctaacggg gccatttctg
    17521 agagtacgtc agaaatggag aaagcgtctc gcatgagcga aattaacttt ggggtatctc
    17581 ttgttttttg gggaaagcaa gaaataagct aatatatgct attaaaagag acacatttca
    17641 aagccagtat agcatagaaa cttttaatga gatagttttt tttacaatta tgagaattta
    17701 tttt
//
LOCUS       DAZAID010000060        17392 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000060 DAZAID010000000
VERSION     DAZAID010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.060"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            285..2051
                     /locus_tag="ACG9BE_001873"
     CDS             285..2051
                     /locus_tag="ACG9BE_001873"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFN3032612.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2153..3412
                     /locus_tag="ACG9BE_001874"
     CDS             2153..3412
                     /locus_tag="ACG9BE_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN3032613.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3559..4860
                     /locus_tag="ACG9BE_001875"
     CDS             3559..4860
                     /locus_tag="ACG9BE_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3032614.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4863..5714
                     /locus_tag="ACG9BE_001876"
     CDS             4863..5714
                     /locus_tag="ACG9BE_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFN3032615.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5727..6542
                     /locus_tag="ACG9BE_001877"
     CDS             5727..6542
                     /locus_tag="ACG9BE_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFN3032616.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6693..7859
                     /locus_tag="ACG9BE_001878"
     CDS             6693..7859
                     /locus_tag="ACG9BE_001878"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3032617.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8089..8736
                     /locus_tag="ACG9BE_001879"
     CDS             8089..8736
                     /locus_tag="ACG9BE_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFN3032618.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8820..9764
                     /locus_tag="ACG9BE_001880"
     CDS             8820..9764
                     /locus_tag="ACG9BE_001880"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFN3032619.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9899..11350
                     /locus_tag="ACG9BE_001881"
     CDS             9899..11350
                     /locus_tag="ACG9BE_001881"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFN3032620.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11371..12207
                     /locus_tag="ACG9BE_001882"
     CDS             11371..12207
                     /locus_tag="ACG9BE_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFN3032621.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12207..12866
                     /locus_tag="ACG9BE_001883"
     CDS             12207..12866
                     /locus_tag="ACG9BE_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN3032622.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12992..13681
                     /locus_tag="ACG9BE_001884"
     CDS             12992..13681
                     /locus_tag="ACG9BE_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032623.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13687..14643
                     /locus_tag="ACG9BE_001885"
     CDS             13687..14643
                     /locus_tag="ACG9BE_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFN3032624.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14687..15199
                     /locus_tag="ACG9BE_001886"
     CDS             14687..15199
                     /locus_tag="ACG9BE_001886"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3032625.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15455..16003)
                     /locus_tag="ACG9BE_001887"
     CDS             complement(15455..16003)
                     /locus_tag="ACG9BE_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFN3032626.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16092..17273)
                     /locus_tag="ACG9BE_001888"
     CDS             complement(16092..17273)
                     /locus_tag="ACG9BE_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFN3032627.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         5722 a         3096 c         3474 g         5100 t
ORIGIN      
        1 ttatgtttca ttcttctaga atatacttct acgccaaaaa ctgttaaaaa acttttactt
       61 tagaaaattg gagaaattga tcgcttaaga taaattcagg acactaaata gtacaggaag
      121 tagactaaaa cttttaaaag ttttaagtct atctatttgg caagtttaaa aaataaaaat
      181 tcggtatcaa aaaactgtta ccgataacat ttttgaaagc gttgtctttt gcttctctat
      241 cacttatagt gatgacgtca tattaaaaca caaggaggaa atcaatggat acagctgcaa
      301 tttatcatcg accggaaagt gaatatgcat ttctatatac gaatacaaga tttcgtattc
      361 ggcttcgtac taggaaaaat gatatcaaaa aagtatatgt gctatgtgga gatccatata
      421 ccataacaac tgaaaaatgg tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca
      481 atgttcatga ttattgggaa attgaagtga cgagcgaaac aagtcgctta cagtatgcgt
      541 tccatgttgt tggggaagat aacacggact gtttttacgg tgatcaagga atttttcctt
      601 atcgagaaaa tgtgattact gaaccaaatt tttattttcg aataccttat tttcatcaaa
      661 tcgatcgatt cactattcca gaatgggtaa aagaaacagt atggtatcaa atttttcccg
      721 aaagatttgc aaacggggac aaagcaaatg accccgaaca cactttacct tggggaagta
      781 aagatccaga ccgagaagac tttttcgggg gagatttgca aggtgtgatg gatcatttgg
      841 attacttgac ggaccttggc atcaatggca tctatttttg tcccatattt aaagccacat
      901 ccaatcataa atacgatacg attgattatt atgaggtgga tcccgctttt ggcgataaaa
      961 aacttttaaa aaacttgatt gatgaagcac ataaaagagg gatacgtatc atgttcgatg
     1021 ctgtattcaa tcatatgggt gtccattcac cacagtggca agacgttctc aaaaacggag
     1081 aaaaatcgat ctataaagac tggttccaca tccgtttttt cccagtagat tcttatcaga
     1141 tgactgactt accagaaact gctgaaaata tcccatatga tacttttgct tttactccat
     1201 tcatgccgaa actaaataca gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa
     1261 cttattggat aaaagaattc gatattgatg gatggcgtct tgatgttgct aatgaagtcg
     1321 atcatcattt ttggaaaaaa ttcagagaag cagttacaga aatcaaacca gacatctata
     1381 tccttggaga gatttggcat tcctctcaag cttggctgca aggggatgaa ttccatgcag
     1441 tgatgaatta tgcatttaca gattcgatta aagattattt tgcaaaaaag aaaatcacag
     1501 cttcacaaat ggtcagtgga atgaatcatc agcaaatgct ttacagagat caggtgaacg
     1561 aaggaacatt caacttatta gattctcatg atactgccag gatcttgacc ctttgccaag
     1621 gtaacaaaga attgatgaag tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt
     1681 gtatctatta cgggacagaa atagggatga ctggtgaaga tgacccggat tgccgcaaat
     1741 gtatgatatg ggagaaggag gaacaagatc tagagctatt cggttttata aaagaacttg
     1801 tttctctaag gaaacagttg tctaagatta tttctgaagg ttctactcag tggctaatag
     1861 taaatgatcg tgaagataag ctctatttca cacggaaact agagggacag atcatctatg
     1921 tttactttaa tcagtcaaaa gaaccttggg tagtagaaca ggaaaatgag gtgattctta
     1981 gtcaaaattg tcagttgtta gaagacggga aagcagagat acgacaatac ggatttttga
     2041 tcatgtgttg aaaactgtta ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg
     2101 cccattataa taagggtgta acagcacgtt gaatacaaag gaggaaatga agatgaaaaa
     2161 aagaagtgtg aaacagttgg gtttagggtt aatctcttta ggaatcttag gcggattagc
     2221 ggcttgtggc aatggtaact cttcagcaga aggggaagcc gatgaaggaa aaacgttaac
     2281 agtttccgtt gattctggat ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa
     2341 agaaaacgat gtgaaaatca agctagtaga aaaggacatg ttcgatcagt tggaatcatt
     2401 agcgttagat gggcctgctg gaaagggacc tgacgttatg atggcagcat atgaccggat
     2461 cggtgcgtta ggacagcaag gacatctggc ggaagtaaaa ctgggaaatg aagcagctta
     2521 cgatgaaaca gacaaagccc aagtgactta tgatggaaaa atctacggag aacctgcagt
     2581 aatcgagacc cttgttttgt actataacaa agatttagtc gatacagcac catcaacgtt
     2641 taaagatttg gagaatctat ccaaagacag tcgttttgct tttgagtcag aagcaggaaa
     2701 aaatacagga tttttagcta aatggactga cttttattat tcctatggtt taattgcagg
     2761 ttacggtggc tatgtatttg gtgatgatgg cacaaatcct tcagatatcg gtttgaataa
     2821 tgcaggtgct gtagaaggaa tctcttatgc aacagattgg ttcaaaaatg tttggccaaa
     2881 gggaatgcaa gacataaaaa gtgcaggaga cttcgccagc cagcaattca tgtccaataa
     2941 aactgcagca atcattgacg gtccatggca agcgcaaaca tacaaagaaa acaatatcaa
     3001 ttatggtgta gccaaaattc ctactctaaa taatggacag ccttatcaac cgtttggcgg
     3061 aggaaaagga tgggtagtca gcaattatgc taaaaacaaa gacctctctc aaaaatggct
     3121 tgattatgta acgaatcagg aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc
     3181 tgcaaaccaa caagctaggg aaacagcaaa ggctaaaaac gatgaattaa caacagctgt
     3241 gattgaacaa tatgaaacag ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg
     3301 gacaggtgca gaaaacttga tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc
     3361 cgcagatgaa gcagtaaaaa caatcagtga agccatagaa caaaaatact gattaaaatg
     3421 ataaccgtcc atttttataa aaaatggaac aggtgcttca gataaaagtg gcaagagcga
     3481 gatggaaaat tatcttgctc ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag
     3541 tttagagggg gcattcagat gcagcaagat aaaacaagca atatcaacga aaaaaaaatc
     3601 acgctttgtt cacttattcc agggatgggg cagtttgtaa atggccaagc ttttaaaggt
     3661 atcgtttttt tcgccatatt cgtcctattc attggtcaga tgcttctggg cggatttcat
     3721 gcattggtag gattggttac tttgggaagt ataccaatgg aagatcattc attatttatt
     3781 ttgattgaag gcactttgca attcatcatt acaatcattt tcttgatttt ttacggtttg
     3841 aatcttttag acgcttatcg agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg
     3901 acgatgaagg aagtcataca taatataggt gatgaaggtt ttccttatct attgacactt
     3961 cccgcttatc ttctcatgat ttttacgatc attttcccag tcctagttac attgttcact
     4021 gcatttacta attatgactt caatcatatt ccaccagcca gtttaattga ttgggtaggg
     4081 gctaaaaact tttttagtat cttcttttta agctcatatc gtaatacttt ttttgcagtc
     4141 ttttcgtgga ccgtgatttg gactttatgt gcaacaactt tgcaaatcac ccttggtatt
     4201 ctcacagcgg ttgttgctca ccagaaattt attaaattca aacgtatttt cggtatcatt
     4261 ttcttattac catgggcggt cccagctttt attactatca tgagtttttc gaatatattc
     4321 aacgatagcg taggtgccat caatactcaa gtgattcctt tcataaacca gcttcctttt
     4381 gtagatatca gtgcaatctc ttggaaaacg gacccgaact ggacgaaagt agcaattatc
     4441 ttagtgcagg gctggttagg atttccctat atctatgtga tggttacagg aatcttacaa
     4501 tcgatttcag aagacttata tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc
     4561 ttttcaaaca tcacgcttcc tgctgtcttc ctagtggcag cgccaacatt tattacacag
     4621 tatacgggga atttcaataa tttctccatg atctatctgt ttaacaatgg cggtcccgga
     4681 agcgtaggcg gaggagctgg ttctactgat attcttattt cctggatcta taaacttacg
     4741 actggaactt ctccgcagta ttctatggct tcagcaatca cgttgattat ctccgtcatc
     4801 gttatctgtg tgtcattgct ggtatttaaa aagacaaatg cgttcaatat gggggattaa
     4861 caatgaaaaa atttcattcg agtgctcgat tccaaaaacg actttctcac ttttttactt
     4921 acttttttct tttaatactg tcagtcatta tcatctatcc gcttttgatc acagcaagta
     4981 ctgccttcaa gtcaggaaat attgcagctt ttagtttaga tctttcaagt gactggactt
     5041 taggaaactt tactcggcta ttcagtgaaa cactttacgg gacttggtat aaaaatacat
     5101 tgatcatttc tgtcctaaca atgatcgtac aagtgactgt agtaactttg gcgggctata
     5161 cttatagtcg ctatcgtttt atcggccgca aatcaagtct gatgttcttt ttagtgattc
     5221 agatggtacc gactatggca gcattgactg ccttttatgt catggctttc ttattagaag
     5281 cgctcgataa gtactggttc ttgacaatga tctatatagg cggtggaatt ccaatgaata
     5341 cttggctgat gaaaggatac tttgacactg taccaataga tttagatgaa tctgctaaac
     5401 ttgatggggc agggcatttc aggatttttt tccagattgt attaccgctt gtcaaaccga
     5461 tgctcgccgt tcaagcatta tgggctttta tgggaccttt tggggatttc atgttagcta
     5521 agttcctgct tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa acatttattt
     5581 ctgatgtgac aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg attgcactac
     5641 cgatttgtct attattcttc tttttacaaa agaactttgt taccggcctg acatctggcg
     5701 gaacaaaagg ataggagcag agtatcatgc aaacatttcc gttaaactat ttttccgcat
     5761 tacgtacgcc aactcaagtg tttgcgggac gcaaactatt atcatggcct aaatttttct
     5821 tgatatttgt atttttagtt tccttgatgg tcatgcctgt gactcttttt tatgccaatc
     5881 agatccaagc gattccatta gaacaatttc tatccgttca ttcgctgatc gatgaacaag
     5941 gaacccaaaa attttctgaa cttgaattat ctgaaactgg gttacaagca tcgcaacaaa
     6001 cgatagctgt gacccctgaa attttggtag gtgtgtcttt atctgagaaa caacaatccg
     6061 atcatggaac ttttattgat tttgagaagg aacaatgggt gatccaacaa aaggataaat
     6121 ctggtatacg ccgctatacg atgaattata gcccatcttt ccaaccagat tctgtacgta
     6181 cgcccgaaga tttccaacga tttttagaaa gagaatttta tgccagtaat cgtccaacaa
     6241 tcattttatc ttatagttta agtcttggtc ttgtcctatt tgtcatgacg agtctcattt
     6301 tattcggggc ttcctttttc ttgtggatga caagaaaaag ccaactttca tccattcata
     6361 cattcaaaga gagtgcaaat ttgatgctga atgttatggg aataggttcc atggcagcag
     6421 cagttgtagg gtttatccac tttgacttta tcttgatgct aagtgtccaa acaacgataa
     6481 ctgtcttgct gcttttatgg gtttttgtca aaacaagatt caaagataag cgtgtcgaat
     6541 gacaagttta agcgatttat gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt
     6601 ttgaaaaatt tctgaaaaaa ttaaacttct tagttgattc ttttcttttt tagtattaac
     6661 gtaatagggt agttttttta aggaggaaaa tcatgttaga gaaaacttta tacaatcaat
     6721 tattttcacg ttctttctcg ctccctgtcg aagtaaatta ttgggacggt acaacgaaac
     6781 aatacggaga tacggacact cctccccaaa tcaaaatcaa tatccatgaa gaaatcccaa
     6841 tgaaagagat tacaagtaac gcatctttgg cactaggaga agcctatatg gatcatcgaa
     6901 tcgagatcga gggaagtatc caagcattga tcaacgatgc ttatcagcaa gcaaacagct
     6961 ttttacgagg aaaagattat ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa
     7021 aaaacaaaga agatatccat gcgcattatg acttaggtaa cgacttttat aaactttggc
     7081 tagatccaac tttgacttat tcttgtgctt atttcaatac acctgaagat acgttggaac
     7141 aagcacaaat caataaagtc catcatatct tagacaaact ctttatcaaa gaaggcgaaa
     7201 cgttgcttga tatcggttgt ggctggggaa cattgatgtt cactgctacc aaagagtata
     7261 atgtcaaggc aactgggatc acactaagtg aagaacaata cgatttgatt accaaaagaa
     7321 taaaagaaga acatttagaa gataaatgcc gtgtattatt aatggattac cgcgaactaa
     7381 aaggcgaaac gtttgatcat atcacaagtg taggtatgtt cgaacatgta ggttctgaaa
     7441 atcttgaagg ttatttcaaa gtggtaaaag atttattgaa accaaaagga acagctttga
     7501 tccatggaat cagccgacaa caaggcggtg cgacaaatgc ttggatcaat aaatatattt
     7561 tccctggcgg ttatgtccct ggtgttactg aattagttga taatattaca aaaaacgatt
     7621 tgcaactgat tgatttggaa agcttacgtc gtgactacca attgacatta gaacattgga
     7681 caaagaattt tcatcaagtc caagatcaag tcacgaaaga aatgggtgat tccttctatc
     7741 gaatgtggga tctatattta caagcttgtg cagcttcatt tgaatcaagt aatatcgatg
     7801 tgattcaata tttgcttgtt caacctggaa acaatgacat cccaatgcat cgtgcataat
     7861 tgaggcaaaa gtactgttta aagactattt aaaaagaagc taagttttat gatgctccag
     7921 aagtgaactt ttttgtctaa cttctaggat tgtctctaaa gcttagtttc tttttcttta
     7981 tattttcata ataaaagttt ggttctttaa tttttaaaca tgacagaaaa ttttttcttc
     8041 gttatacttg taatagaata ctattttata acaaagggga tcgaatcaat gaaaaaaact
     8101 gttatttata ctagtttatt tgcagctttg tttttagctg cttgtcaatc aaataccaat
     8161 caaggcacat ctgggaatac gtcttccagt agcgaacagc gctcttcaca ggtatcggac
     8221 tcctcaacaa aagcaagcac atcttcgtct agccagacaa aaagttctag tgaagcaagt
     8281 actcagtcag atatcaaagt gtctgcagct cgtgcgatcg aattgttcca agataaatac
     8341 ccagatactg caattactag tttggaatta gatagtgatt ggggatctta cttctacaag
     8401 atagaaggtg tcgatgatca aaaagaatac gaagcaagaa ttgatgctca aaatgaaaaa
     8461 atggagacag agaatccaga aactctagat cgagatgaac aaaatggtca aaagaaaaaa
     8521 gaagatggtc tggatgttga taaattgatc acgattgagg aagcagcaaa aatcgccgtc
     8581 gacaaagtag gaagcggtac ggcaacagat tgggatttgg ataaagattt aggtactact
     8641 tattgggaag tcaaagtaaa agacggcaat caaagtacat cagtcaagat caatagccaa
     8701 acaggcgaag tactgtcaac tgaacaagat gattaagaat ttcttcggaa aacaagagtt
     8761 gatttttctt ttagtcatga tacactagac aaaataactt gtttgaagga gcgatttact
     8821 tgagtttaga aacgaccttt gccaatcata tctttgccaa tcctttgatg aatgcatctg
     8881 gtgtccactg catgactacc caggaattag atgaactggc tcattcagaa gcaggagcat
     8941 ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa tcccgaacca cgctattttg
     9001 atgttccttt agggagtatc aattctatgg gacttccaaa cttaggtttt tcttattatc
     9061 tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca aaatcaaccg ttattttttt
     9121 ccattgccgg gatgagtgtc caggaaaact tggaaatgct tgagaaaata gaaaaaagtg
     9181 ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa tgtgcctgga aaaccacaac
     9241 tagcttacga ttttgaggcc acttatgaaa cactgaaaga agtattcagc atcttttcta
     9301 agccgctagg aatcaaattg cctccttatt ttgattttgc tcattttgat cagatggctg
     9361 atatcttgaa tcagtttcca ttgacctatg tcaatgctat caacagtgtg ggcaatggtt
     9421 tgtatatcga tacagaacaa gaagcagtag tcatcaaacc aaaagaaggg ttcggcggca
     9481 ttggcggaga gtatatcaag ccgactgctt tggcgaatgt tcgcgctttt tatacacgtt
     9541 tgaaaccaga aatccaaatc attggtacag gtgggattcg gacaggacaa gatgcatttg
     9601 aacatctgct atgtggcgca agcatgttac agattggtac cgagcttcat aaagaagggc
     9661 cggaaatttt ttctagaatc ataaaagaat taacgcagat tatgtcagaa aaaggatata
     9721 cgtcaattga cgaatttaaa ggcaaattac gcaccatttc atagcaaaga gacgaaggtc
     9781 atgattgatc ttcgtctttt tttataaaaa acactcgtct gttcattcct gtgcaagcgt
     9841 actcattgta tgttagacga acttgtggga aaataacaga ggcaaaaagg aggataatat
     9901 gaatgatgca acagaattag caaaagagct taaagaaaaa gaatttagtt ttcaagaact
     9961 agttacggat atacagaaaa aaataaagaa gcaaaatcat gaattgaatg cgtttgtcgc
    10021 gcttgaacca ttcgagagta attcccacga gctattgatt ccccagtcag aaaaagggcc
    10081 attggctggt atcccatttc ctttaaaaat gctcggtcaa gaaaaaaagg gatggctagc
    10141 tacttcagga tctcgtttat ttgaaacaca ccgagcttct catacatcta attatgtgcg
    10201 gcaagcagaa gcgataggtc tagttccttt tggacaaacc aatgcgccag agtttggttt
    10261 caaaaatatt acagacccag tcatctatgg cccagcaaga aacccttgga accttgaatg
    10321 tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt tcatcaggaa tcgttccttt
    10381 agctggtgct agtgatggcg gtggttctat tagaataccc gcttcgtttt gcggattgat
    10441 tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca tatgcttgga gaggatggca
    10501 aggagctgct attgatttcg gcttgacagt ttctatgaga gacacggagg ctttatttta
    10561 tgggatgaga agtatccatt ccggtgcgcc atatcaagta tcgccagttg agtggcaaac
    10621 acatccaaga aaaaaacggt tgaagatagc tttttgtgtt gattcaccga tccactcaca
    10681 gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta gatttccttg gaaaagaagg
    10741 gcatgatttg acagagattc cttacccact agatggtcgt aaactgatca cgtcttatta
    10801 tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg attgaaacag gcatccagcg
    10861 agctgtcaga aaagaagaac ttgaacccat ttcttggact ttgttccaat atggtcagaa
    10921 gctctctgcc gctacttaca tcgattctct gcatgtatgg gatgaagcag caattgtcat
    10981 ggaaaaccta catcaagaat acgatctatt cctttcacca acgactgcat ttaccgcccc
    11041 taaaatcagc gaagatttgc aaagtgatgc gataagagca cagatggaac acatcagtga
    11101 ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg tttgaggaga gcttgaagat
    11161 cacaccttat acacagttgg ctaatctgac tggacaacca gcaatcagct tgcctactca
    11221 tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg gctgccagag gaagagaaga
    11281 tttgctattt caaatcggct atctatttga agcaaatcat cagttccatt taccaaatcc
    11341 atggaaatag cagaaacaag gagaagaaga atgaagaaaa aaagattgtt tgctctaata
    11401 actagtgcct tttttatttt aagcgcttgc ggtacagctg ccgctaatca ggaagataac
    11461 agctggagta atgtagaaaa aaagaaagaa ttagtagtag cgacttctgg cacactgttt
    11521 cccgcatctt attataatga tcaaaatgaa ttagttggtt atgatgtaga tttggcaaaa
    11581 gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag aatacaacgt ggatggacaa
    11641 atcacttctg tttcaaaaag agaatcagat tttgctgcga acgattttgg tatcacaaaa
    11701 gaaagagaaa aaaaattctc cctttcagag actttcaaat attcatttga cagtatgatc
    11761 gtacgaaaaa aagatgattc aggaatccac tcacttgaag atcttaaagg gaaaaaagca
    11821 gctggcgaac ccaatactag ctatatgcgt ttagcggaaa aacacggagc taaattagtc
    11881 acatatgaca atgccacgaa tgatcagtat ttaagcgatg tagcaaatgg tcgaacagat
    11941 gttattctaa atgattatta tctgcagaaa atggccgtat ctgctttacc agatatccct
    12001 gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa gtggtttttt attgaaagaa
    12061 ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa aagagatgaa agaagatggg
    12121 acgacaaaga aaatatctga aaaatatttt caaactgatg tttcagtcaa accgaaagaa
    12181 acgatggctg agtcggtgag tgagtgatgt actttcctct aattgatttt tccctcatgt
    12241 ggacttcact tcctttcgta ctgcaaggct tggtatacac attagggatt ggcttcgtgt
    12301 cgtttctttt aggaaatctg gtagggttgc ttttgacagt tttaggtctt ttggattggc
    12361 tgcctctgaa tgtatttatt cgtttttatt tatcgttctt tagaggcatt cccgcactag
    12421 tcctgctgtt tctgttatat tttgggttac cttatcaatt aagtgcctta acggcttcag
    12481 tcatctgctt tacgattacc agcagtgcat ttattggtga aatctatcga ggatcactag
    12541 caggcgtttc ttcaggtcag tgggacgctg gtttagcttc tggtttttct ttctttgggg
    12601 tattgaaata tatcgttttg ccgcaagctt ttcgaatctc agtaccggca ctcagtaacg
    12661 tagctatgga tctagtgaaa ggtacttcgt tagcagcaac aatcacagtg ccagaaatat
    12721 ttcaaaaagc aaagatcata ggcggtcgga cattcgatta tttttctctc tatattttag
    12781 ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa agtgcaacag tatttagaaa
    12841 aacgttctat gcatcagtca atctgaaaaa tagaaatggt gctagcgtgg ctggcaccat
    12901 ttctattttt tgtttcttgt atattctgcc tagtatttta aaaccatttc atgtaaaatg
    12961 taaatcgaaa atatgttcgg aggtagaaaa catgaagacc ccgctaacca tcgaaataga
    13021 atcctcgtta taccaatatt gcatcgagca aggcggcttg gttgtagaag aagtcacgat
    13081 gcctgatgac caaggaattg tagatacgct tagctgcttg acaaaatttg atggaagccg
    13141 agaatggcga tgctatgagc tgaaagtgac aaaagcagat tttcgctcta cagccaaact
    13201 gtcatttgtt ggacattata attattttgt tttaccaagt aaactatatg aagaagtaaa
    13261 aactgaaatc cctacaggaa taggtgtttt agtgtatcgt ccgtacacgg ttgaagagga
    13321 gatgcctgcc tctggtacat ttctgattgc gaaaaaagca gcaaagaaag agcttgctgt
    13381 accggaagaa gctttgacaa accgcttcat ggcttcttta ttcagggaag taagaaaagc
    13441 aaagcaaatg gagcgaggcg taaaatattt tccaacagat caattgtaca aagaattgaa
    13501 aagacgttca gaaaatcaag atccattttc taacgaacga tattatgaac ggtttttaga
    13561 ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa gttatcgcgc ttcagcatga
    13621 ttatgaatat ttgaggcaac aacagcagat ccgtcggttg cctacagagc cattggagtg
    13681 aaaaatatgt actatcctta tttaagagga aaacaatttg acctattagc attaaaagaa
    13741 gcactgtcgc gaggattgct ctccaataaa atccaaccag tgatcgaacc agtacgtgat
    13801 tccgctacgc ttaaaaatgt gatcgaatta tttcaaaaaa agcaccatcg aatcgctgtg
    13861 atccaaaatc cccaagtagg acaattcaaa ttattcgatc agcatgtgca cagttggaca
    13921 gtcaaagaaa atagctctgt cgtccctgca cagatcttaa cgccagaaaa tctcagtgaa
    13981 gtactggatt cacctccagc gtttcttgtt ttcgatggac agcactaccc aaaagataca
    14041 gaggtgtgga aacagttggc aggagttgat tcgaaatttt tgataccaga tactagtcgc
    14101 tttcgcatct ggctgcccga aaacaaaatt gtgatccgtg actcttttca aacgagaaag
    14161 catgttgaaa gttatgcaga taaaacagat gactttttta gtgatgacta tctttttttc
    14221 cacgcagacg gatatattgg attttctgat ttcacgatcg aaggtagccg ttatttcgat
    14281 aaagggttcc ctagccgagc gcttgctatc catctaacgt atattgatgc ttacggcaat
    14341 atacgcgtga aacattttgt ctctgacagt aacgactctg ctaaagacca agcattgaag
    14401 tttcttgaag caggagataa aatgaaaaaa tggatcatgc gtcaccatca tcaactcttg
    14461 attacttcag gaatgattga attattggct ctttaccagc aacaaaaatt ccctggactg
    14521 ggagtgttga aaaagtggtc acttatgcat catttggaac ttattagtca cctgctagat
    14581 catccaaaag attggcttaa atattattca aaagttcagg aactatatga agtgatccaa
    14641 tgattagata atgtaaatga aaaaggaata gaaaagagga aactggatgt atattcggca
    14701 aacaaggaaa gaagaagtag aagaagtggt agaaatcatt aatgccgcag cggatttcat
    14761 tgcttcgcaa ggctctcctc aatggcaaaa cgggcagaaa ccaacaaaag aaacgatttt
    14821 gaacgatatt gaaagaaaag aaagctatgt attaatagtg gaagatcaaa ttgctggaac
    14881 agctgcatta gtctcaagcg tggatcccgt ttacacaaaa atagatggta gttggaaaga
    14941 atcaactggt ccttatttat cgattcatcg tgtcgcttta gcagaaagta ctcgaggaaa
    15001 aaaactagca aaaccgttct tagaacattt gttgacgatt gcagccagca aaaatatcca
    15061 agatatccgt atcgatactt atccagctaa tgaaccgatg gaaaagacca tctattctgc
    15121 tggattcact tatcaaggga tgattgaatt tccttttgaa cacggcgaaa gaaaagcata
    15181 tcaaataatc atcaattgac tataaaatca tctgagcaaa gataaagaca ttaacactac
    15241 tgaaaaaaac aatcatatga tagccaagag gttgtaacag aagtggccag cttcaaaaaa
    15301 caagaaagaa tttccgaaaa tgcttctcaa atttttgtgg aattttagcg tattttacga
    15361 aatagctgct ttctgaaact cccttcatcg agaggtcgtg acatagcttt tgtcacgacc
    15421 tctcggctta tcctacgagt attattcgat acatttatat caataatcct ttttttattt
    15481 tatctgcagc ttcttggaac gctccatcgt ttgacacgac taaaagtgag gggatagctt
    15541 tcaaacgttt caactgtgct aaatcttcta cataaaccgc cagtctggct gaaatctgtt
    15601 cagatgaatc tccacgtttt ttcaggcgcg attgcaatgt ttctttagag atcgtaataa
    15661 agataggacg gactaccgtc ccgaaatact catataaatg ccaaaaacca tttgctgtca
    15721 aaacggcgta acaatttcct actgtcagtt tctttttaat ttctgcttta ctggaaccat
    15781 atttatgttg agcatattgg tcccactctg caaactcatt tcccttgatc atctgattga
    15841 atgtattgtc agaaacaaaa taataatcct cttgatcttt ttccccaggt cttggtgatc
    15901 gattcgtgta agtaatgatc ttttgttccg gctgaaaaat agcttctgcg agtgaagttt
    15961 ttccagaacc agaaggaccg ataaatacat agcaataatt cattgtcatt tctcctttga
    16021 tgattgaaca agtgaaaaag ctttggaaaa tatttccaaa gcttttaaaa gtaactttgc
    16081 ttttttgtat attataccag caagccagtc acaatagcac ttaaaaagct gactaaagtc
    16141 gctgttagca aaattttcaa tccgtgtttt gcgacaagat ttcctttttc ttcatttaat
    16201 cccttcatcg caccagaaat gatcccgata gaagagaagt tggcaaatga aacgaggaag
    16261 acagatataa ttgctgttgt acgctcagta aaatggaaat taccatttgc taattctgtc
    16321 atagcaacaa attcatttgt aaccagttta gtcgccatga tactaccggc atccactgct
    16381 tctttccatg ggattccgct gatgaaggca agaggggcaa agatgtaacc taggatctct
    16441 tggaaagtga tgccgaatat cccgtggaag atagcattga tcatggcaat caaagcaaca
    16501 aaaccgataa gcatagcagc aaccgtgatc gccacatgga aaccatcaag aatgtattct
    16561 cccaacattt ggaaaaatgt ctgttctttg ttttcttcaa tgatcagctc atcttctttt
    16621 tcttctaata catagggatt tacaattgaa gccagaatat aaccgccaaa taagttcaaa
    16681 acaagggcag tgatcacata tttagaatcg atcagtgcca tataagatcc gacgatcgac
    16741 atagaaactg tagacatagc cgatacactc atggtgaaca aacgtttctc agataggaag
    16801 ggtaattcct tttttatgga aataaaaact tccgattgtc ctaaaatagc agaagctact
    16861 ccattataag attccaattt gcccatcccg ttgattttac ttaatcctag accaacagct
    16921 ttcataaata atggaagaat cttgatatag cgtagaatac cgataatagc agaaataaat
    16981 acaattggca tcaatacgga aagaaagaaa ggtgcacttc ctttatttgc tacaccggca
    17041 aaaacaaagt caacaccttg cccagcaaaa gcaagaagat gatcaaacat ttttgccaat
    17101 acagaaacaa ccgctgtacc aaaagttgta cgtaataata cgaaaccaag aacaagctgt
    17161 aaaacgaaca tcacaattag tggtttcaat ttgattgctt ttttgttact gctgatcaaa
    17221 aaagcaagcc ctaatgtgac aaataaacca ataataccta ataaataact caaataatca
    17281 ctcctattcc aaattccgtt ttattttacc tgttttgcaa ataaaatact aatctttagt
    17341 gaaattttca ttactttttt agagtgaaac gcattcattt tccattagaa aa
//
LOCUS       DAZAID010000061        16749 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000061 DAZAID010000000
VERSION     DAZAID010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.061"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..283)
                     /locus_tag="ACG9BE_001889"
     CDS             complement(<1..283)
                     /locus_tag="ACG9BE_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN3032628.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYR"
     gene            complement(285..482)
                     /locus_tag="ACG9BE_001890"
     CDS             complement(285..482)
                     /locus_tag="ACG9BE_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFN3032629.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(876..2603)
                     /locus_tag="ACG9BE_001891"
     CDS             complement(876..2603)
                     /locus_tag="ACG9BE_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFN3032630.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2596..3789)
                     /locus_tag="ACG9BE_001892"
     CDS             complement(2596..3789)
                     /locus_tag="ACG9BE_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN3032631.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3976..4620)
                     /locus_tag="ACG9BE_001893"
     CDS             complement(3976..4620)
                     /locus_tag="ACG9BE_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3032632.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4714..4848)
                     /locus_tag="ACG9BE_001894"
     CDS             complement(4714..4848)
                     /locus_tag="ACG9BE_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFN3032633.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4850..6982)
                     /gene="feoB"
                     /locus_tag="ACG9BE_001895"
     CDS             complement(4850..6982)
                     /gene="feoB"
                     /locus_tag="ACG9BE_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFN3032634.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6979..7452)
                     /locus_tag="ACG9BE_001896"
     CDS             complement(6979..7452)
                     /locus_tag="ACG9BE_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFN3032635.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7853..8077
                     /gene="nrdH"
                     /locus_tag="ACG9BE_001897"
     CDS             7853..8077
                     /gene="nrdH"
                     /locus_tag="ACG9BE_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFN3032636.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8236..10395
                     /gene="nrdE"
                     /locus_tag="ACG9BE_001898"
     CDS             8236..10395
                     /gene="nrdE"
                     /locus_tag="ACG9BE_001898"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFN3032637.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10445..11410
                     /gene="nrdF"
                     /locus_tag="ACG9BE_001899"
     CDS             10445..11410
                     /gene="nrdF"
                     /locus_tag="ACG9BE_001899"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFN3032638.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11499..12638)
                     /locus_tag="ACG9BE_001900"
     CDS             complement(11499..12638)
                     /locus_tag="ACG9BE_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN3032639.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12947..13660)
                     /locus_tag="ACG9BE_001901"
     CDS             complement(12947..13660)
                     /locus_tag="ACG9BE_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFN3032640.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13914..14615)
                     /gene="nagB"
                     /locus_tag="ACG9BE_001902"
     CDS             complement(13914..14615)
                     /gene="nagB"
                     /locus_tag="ACG9BE_001902"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFN3032641.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14849..16381
                     /locus_tag="ACG9BE_001903"
     CDS             14849..16381
                     /locus_tag="ACG9BE_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN3032642.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16572..>16749)
                     /locus_tag="ACG9BE_001904"
     CDS             complement(16572..>16749)
                     /locus_tag="ACG9BE_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3032643.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5200 a         3448 c         2885 g         5216 t
ORIGIN      
        1 ctcgatataa accatttatt ccagcagttc ttccaatttc actcaaattt cttattaatt
       61 cctcttgata ttcagtgaat cttattttat tagtataagc agtaaaatat agataattca
      121 ccaatctatt tattaaagga ctgggatcta attcatattt ttgtttttct aattttaaat
      181 ataccttttg taaaacctca agaatatcag aatatttagg atctttactt atagatatat
      241 cattatataa ttcatgaaca atttgcttag catttttttt cattttagca cttccctgga
      301 attgctccac ctaaaaaacc acctatagac attcctgcaa tacaagtagt tgccttggcc
      361 caatcgaccg tacatttatt tttagtgcaa tacactccat ttccataata ttttccacta
      421 tgagtggtac ccccatagat aagttgtgtc tctttaatag acaaaatttt taaatgtttc
      481 ataatctaat cacccctaaa ttaatgtttt tagactatta ttctaacatt cattaaattt
      541 caaaaaacaa ttccttcttg aaacgtagca aaataattcc tgaataagaa tttaatcact
      601 tttaagtata taaactatgt atattcttta atattttttg agaatgcact tcgtacataa
      661 taaactttaa tcacattagt ctttcatacc tacgatcgat ctctttgact cgcacccata
      721 aattcataat agtttgtcgg cgtctttgaa cgtttcgaag atgagctacg cattctcttt
      781 ttttatagac aaaaaagata gagacgaaca tggagcttcc aaatttggat atttgatcca
      841 attttggaag ctccatttat ttcgtgtctg tctttttatt ttttccagct ttcgactaat
      901 tcttggttct ctttgatcca gtcttttgct gcttgctgtg gatcttttcc attgttgatt
      961 tccaacataa ctttttccat atctttttct gaccaatgga aattgtctaa tactttgtaa
     1021 gcttctggct ggtcttcttt caatcctttt cgaaccatcg tatggatctg ctcttcgctt
     1081 cccattccgt tttcaggatc ttctaagtat ttcagatcat atttggcaaa catccaatga
     1141 ggtgtccatc ctgtaactac aattggttcg tgttttttga tcgcttgacc tagtgcaacg
     1201 gtcattgctc ctgaagaaga tgtctctact ttccagtctt tcaagttgtc atatttttgg
     1261 atcgtgtttt cagcagccgt aactacacca gctcctggct cgattcctat gattttctta
     1321 cctgcctgat cggttaactc atcgatcgaa tttacatcca tgtaggctgg cactgctaaa
     1381 ccaacttttg ctcctgtcaa attggcacct aggtcttcga cttgatcttt atattgtgca
     1441 tattgtgaac catgagtttt aggtagccaa gctgaaacca tggcatctga ctcgccattt
     1501 gaaacagatt tccacatgat tgaattatct aatggcgtca tcgttacatc atagcccatt
     1561 tgtttcaata cttcaccgac tacatttgtt gaagcaactt ctgtatccca ttcaacataa
     1621 gataagttga tacgttttgt ttctttggca gatgaaaaac tccctatacc aatcgcacct
     1681 aagatcacta ctactacagc accaatgatc agtccttgac gttttttact tttctgtgtt
     1741 gttgctcctg cagctttttt attgtttggc tggttcaaat gctgtgtaaa acggtcaatg
     1801 atgattgcta agataactaa tgcaaccccg ttgacaaatc cattcccgac ttgtgctctt
     1861 tgcaatgctg ataatacgcc gcgtcctaag ccgggtgcac cgatcataga ggcaataacg
     1921 accattgaaa gtgacagcat tgttgtttga ttgatccctg ccatgatcgt acttttagct
     1981 aaaggcaatt ctagtttgaa tagtttttga cgacttgtgc tcccgaatga atcagaagct
     2041 tctactaatt ctttaggaac ttgtcgaatc cctaaattcg taaaacgaac ggttggtggc
     2101 agagcaaaga tgactgaggc aaatacccct ggaaccatcc cgataccgaa aaatgcaact
     2161 gctggaatca aataaacgaa tcctggcatt gtctgcataa agtcaaggat cggtttgata
     2221 atactttggg ctttactgct ttttgccatc aagatcccta atggtacccc aatgatgatc
     2281 gagatcacac tagataaaag aacaagtgtg acagtattca tcaaatcatt ccatagacct
     2341 tggttataaa taaaccatag accgattaat gtgaacaacg taagtcctgg acgtttctta
     2401 gaaataaaga aagctgctac cgtcaataac aagataaaca aaggagctgg aatcactaac
     2461 agtaaatttg taatccccga catcactgcc tgtccaatcg tttgtaaaaa tgaaaatagg
     2521 ccagaaaaag ttgtcgtaaa ccattctgtg atattttcta cccagtttgc cactggtaat
     2581 tgatagttca atagattatt catgttcact cacctcgctt gtttctgcta atgcttcaat
     2641 cacactacct cggatgacga caccttttaa ttttccatcc tctacaactg caagtggagt
     2701 cggtgagtca taaataatat tgaaaatatc acttaccacc atgtctttcc caatcaccgt
     2761 cacattttca tctaggaagt cgatcaatgg acgtttttgc ttcctagctt ctaacgcttg
     2821 atctgcagtg atgatccctt tcaggtgtct ctttctgtca acagccatca gcatgctgac
     2881 ttcttcatta cgcatccttg tcaatgcaac gtttggtcca tcagattcga tattcgtcgt
     2941 caatgcaggt accatgatgt tttgagccgt aagcactttg gaacgatcga cttcttccac
     3001 aaattcacgt acataatcat ttgctggatt ggtcaatatt tcttcacctg ttccgatctg
     3061 catgatttcg ccgtctttca tcaaggcgat tctgtcccca atccgtaaag cttcattcaa
     3121 atcatgggta atgaaaataa tcgttttttg aacattggct tgcaggtcca gtaattcgtc
     3181 ttgcatttct cgccggatca gcggatcaag agccgagaac gcttcgtcca tcagcaagat
     3241 ctcaggatca tttgctaaag ctctggctag gccaacccgt tgttgcatcc caccagataa
     3301 ctgactagga tattggtctt taaatgaaag caaactagaa ttttctaatg ctttttctgc
     3361 tttttcttgt cgttcttctt taggtacacc acggacttct aacccgtatt ctgtattttc
     3421 caagatcgtt cgatgtggaa acagaccgaa gttttggaaa accatattga ttttatgacg
     3481 acggacttct cgcaattctt ctttgcttaa cttcgataca tcttctccat caatatagat
     3541 actgcccgaa gttggctcaa tcagacgatt caatagacgg atcaatgtgg atttcccact
     3601 cccggaaagc cccatgatca cgaagatttc tccttctttt acgtcaaaat tcacgtcata
     3661 cacaccaacc gttgcaccgg ttttctttaa aatctccgtt ttcggctggt gatcattcat
     3721 catatctaaa gcggcttgtg tctttttacc aaatatcttt gttaaatgtt ttacttctac
     3781 ttttaccaaa caaattctcc ttttttatgt aaattcattt ttctttctga aaaaagtgac
     3841 cacactatgt taacatcatg tagtcacttt gtcaaaaatg aaagaaattt gcttgtccaa
     3901 actgaaaaaa acttggctaa atctttgtaa ataaaaggtt tagccaagtt aatatttttt
     3961 taatatttca cacaatcatt ctgcaagctg atgctccggg taaaaaaagt ttgtcatacg
     4021 ctgtagtgta tattcatttc caataaaatg aacgatgtcc cctgccgtaa atttagcata
     4081 ggggccaggt gaaacaagca gttcttcatt atgagaaatt gcgataactg tggcgccagt
     4141 cgcctgccag atattcaact cgctaattga acggtccaga tgcattgctt tttctgataa
     4201 agataattca aacggaagta aaggattcat cttatgtatt cgcttcgttt gattgaccag
     4261 ctgatccaat aattgagaaa aatgattcag ctcatcttgt tgcttctcca cacttgccat
     4321 gatttctgct tttgtatctt ggatggattg tacatcttga tattgcaata ggaaatcttt
     4381 tgctttttcc ttcgaagcaa caaatgcccc acttccatgc cgtacttcca tgattccaag
     4441 atctaccagc acattgatag ccttgcgggt agtctcagga gaagcattgt acgtactggc
     4501 aagtgttgag cgggcatgga tcttctcacc cactttatat cggttttcaa cgatacgttc
     4561 ggctaaatca acagcaatct gctggtagcg cgggcggacg atttttttct ttccagacat
     4621 tttctttcct ctttcttttt ttactttccc tcatcttact atgctctagc aaaataagca
     4681 aaaaagggga gaatagcctc ttgcaacgac tgatcatcgc ttgatctgtt ccttttttac
     4741 tgggcaagct gtttgacagt catcacaatt cttaccggat tttaaacgag tatagacaat
     4801 tgcgcctgca cttccaaaga ttaacacact taataagaat gtcgccatcc tattcctctc
     4861 cttctaatga caatatagta taaaactggt tcttggattt tggctgacgg aacaaaacat
     4921 aaacgagtcc tatcatcagt aaaatagcaa gatacgtccc taacgctacc tgtccaccct
     4981 ccaataagtg tcccaactga taaatcacaa aactaactaa ataagctaaa ccgcattgat
     5041 agcctatggc gatccaagtc catttcttgg cattcatttc ccgatggata gccccaattg
     5101 ccgcaaaaca aggcgcacaa agtaaattga aggctaacaa tgaataagct gctgcgggag
     5161 tatatgtgtg ttgtagcact tgccagattt ctctgccatt ttcggatact tccgcatgac
     5221 caaacaaaat accaaatgtg ccaataatat tttctttagc gaccaatcct gtaatcgctg
     5281 cgactgttcc ttgccagttt ccccaaccaa gcggctgaaa aacaactgcc aggattcgcc
     5341 cgaaaaaggc taagatactt tggtcttcag aaacaggttg agcataaaaa ttataagaag
     5401 agctgaacca gatcacgata ctagaaacaa agataatcgt tcctgctttt ttaacaaaag
     5461 acttgctgcg gtcataggtt tgtcttagga ccgctgacca ttgaggaaaa tgatatgcgg
     5521 gcaattccat gataaaagga gctgtatctc cagcaaacaa acgtgttttt ttcaagctga
     5581 ttccagagaa aataattgca gtcataccaa gaaaataggc agaaggagct acccaagatt
     5641 gatgaggaaa aaaggcccct gctactaagg caataatcgg caactttgca gaacaaggca
     5701 tgaaggttgt gaccatgatc gtcaaacgac gatcttgctc gttttcaatc gtgcgactgg
     5761 ccatgacgcc tggcactcca catcctgtag caatcagcat aggaatgaag gattttcctg
     5821 aaagaccgaa tttcctgaaa agtcgatcca tgacaaaagc gatcctcgcc atatatccgc
     5881 agtcttccag aaagctcaag cataaaaaca atataatcaa ttgcggtaga aatcctaaca
     5941 ctgctcccac gcctgcgatg atgccgttca agatcagctc ctgcatccat cccgctactt
     6001 gccattcagc taacaatctg ccaacatgtt ctggtaacca tgtaccaaac aatgtgtcat
     6061 tgatccaatc tgtccccatt gttcctactg tttgaatagc cagataatag atcagccaca
     6121 tcacaaatgc gaagatcggc aaggctaacc aacgattcgt tgcaatctga tcgattttgt
     6181 cactcatcga taattggaaa gaatcatttg acacaacaca gagtgtacat agttgggtaa
     6241 taaattcata acgttcattg accagaatcg tctcactatc gtcgtccaat agtttctctg
     6301 ttaactggat cagttgctcg atctcttttt gctgagaaac tgctatctcg taatcctctc
     6361 ttaccttctg atctcgttca aaatatttca ttgcatacca gcgtttcttg ccgctttgtt
     6421 ctcctagtac atcttcaatt tcatgtaaag ctgcttctaa acgtggatca tatgtacagg
     6481 gaacaggaat agaaggccgc ttttttgcag actcctgaat cttttcgatt agtttttcga
     6541 ttccttgctt tttcaatacg ctgattggta aaactggtac accaagacca taggaaagct
     6601 tttctagatt gatttttttt ccttcttttt tgatgacatc catcatattc agaccaatga
     6661 tcataggaat gtctaaatcc aataactgcg tagttaaata aagatttcgt tctaaattgg
     6721 tagcatcaat aatgttgatg atcagttcag gctgttcctg aaccaaataa tctcttgcaa
     6781 tcacttcttc tggtgtataa ggagacaaag aatagatgcc gggtaggtcc tgaatatgaa
     6841 tgttcggatt ttttttcaac gatccttctt ttttttctac tgttacgccc ggccaattcc
     6901 ctacatattg attcgaacca gtcaagagat tgaacaagct agttttcccg ctgtttggat
     6961 ttccgactaa cgcgatattc attctttttc ctccctttgt atcaagatta gttcggcctc
     7021 tgactttcgt aacgtcagtt cataccctcg cactgtcagt tcgattggat ctcccagagg
     7081 ggctagtttt cggataacta tttttacatt tcgcgtcaat cccatatcca ttaaccgtcg
     7141 cttcactgcg ccttgtccgt ggattgctac gacgatccca gtctctcctg cttttaattg
     7201 atcaagagaa gtccagcttt cagaggaagc agtcttttta ttgacaaaga tttgacctag
     7261 tatttcccga cttactgcca ctcgatgact ttttaccagt aaaatggcat tgttgcctga
     7321 taaattgacg acgacgattt tacttccggc aacgatcccg atattttgca aatggcgttt
     7381 tgttttttct tctgtttcga ttcgttcgac ttgataaacg aaattctgct ttgcctgcgc
     7441 taaggtctgc atattgattc ctctcttgtg aaataaatca tgtattaaaa tcattcatta
     7501 tatgttctaa ttttcttacg tgttacgcaa ataaatgaga atcattctca catagaatac
     7561 tctcggtaat tgagaatgtc aattatactt gtgatattat gagcgaatct ttagtattga
     7621 tagataaaaa tgagaaaaaa atatttcttc atagttttcc acaatttttt ctctattaaa
     7681 tttgctattt tcccacacga tacttgtttt cagctctttt ttcaaaaaca caaaacttag
     7741 tattccacag tttccacaac acaatatgta gtgtttgttg tttgactttt ttactaagag
     7801 ttttatacta tctattatag ataataagaa tgtgttagag gaggcactca ccatgaacgt
     7861 aaaaatcttt tctaaaaata attgtatgca atgtaagatg gtcaaacggt ttttaacaga
     7921 aaatcaaatt gcttttgaag aagtcaatat cgatgaacag cctgaagcag tagactggtt
     7981 gaaagcacaa ggattccaaa gtgttcctgt cattacttct gatgcagaaa cagttatcgg
     8041 attccgtcct gatcagttgc gtaaacttgc cagctaacat gactaaaaga atcaaaacgt
     8101 gatacatgga tttgaagggc ttgagacagc accagttttt gtcgcaaact tccaaatcct
     8161 tttttaaagc ttaaaaacaa tgcaatgatt tgcaatcaag catcatcaaa tacgaaatga
     8221 aggttgggcg tacgtatgag tcttaaaaat ttacaagatg tcagttattt caagctgaat
     8281 aatgaaatca atcggccagt caatggtcaa atccctttga ataaggataa agaagcttta
     8341 gttgcctttt ttgaggaaaa tgtcaaacca aatacgatga cttttccatc tattatggaa
     8401 aaaatcaatt acttgatcga tcaagaatat atagaagcag aattcattca attgtattca
     8461 ccatcattta ttgaaaagct atatcaattt ttggacgagc aacatttttc tttcaaatct
     8521 tttatggctg cttacaaatt ttattctcaa tatgcgttga aaaccaacga cggttccgct
     8581 tatctggaaa cttatgaaga tcgagtagcc ttcaatgcgc tttactttgc gaacggggat
     8641 gaagaattag cacttgctct tgcagacgaa atgatccatc aacgatatca gccagctaca
     8701 ccttctttct tgaatgctgg acgtaaacgt cggggcgaat tggtgtcttg tttcttgact
     8761 caagtcactg acgatatgaa ctctatcggg cgctcaatca actctgcttt gcaattgtcc
     8821 cgtattggtg gcggcgtagg gattacttta tcaaatcttc gtgaagccgg cgctcctatc
     8881 aaaggatatg agggtgctgc aagtggtgtc gttccagtca tgaaattatt cgaagatagt
     8941 ttcagctatt ctaatcaatt aggtcagcgt caaggagctg gcgtggttta tttgaacgtt
     9001 ttccatccag acattattgc tttcctttca gcaaaaaaag aaaatgcaga tgaaaagatt
     9061 cgcgtaaaaa ctctttctct tggcgtgatc gtacctgata aattttatga attgacacgt
     9121 aaaaacgaag atatgtattt atttagtcca tatagtgtcg aaaaagaata tggtgtccca
     9181 ttctcttatg taaatattac agctgagtat gatcatctag ttgcaaatcc aaacattcgc
     9241 aaacaaaaaa tcaaagcgcg tgatcttgaa aatgaaatct caaaattaca acaagaatct
     9301 ggctatcctt acgtaatcaa tattgataca gcgaaccgcg agaatccaat cgatggcaag
     9361 atcatcatga gtaatttatg ttcagagatt ttacaagttc aaacgccttc tgtgattaac
     9421 ggcaaacagg aatatgaagt attaggaaca gatatctcat gtaacttagg ctcgacaaat
     9481 atcgtgaatt tgatgcaaag ccctaacttt ggtcaatctg tgcgtacgat gactcgtgca
     9541 ttgacattta ttacagatgc atctgatatc gatgtcgtac caccgatcca aaacggaaat
     9601 aagctgaatc atacgattgg attaggtgcc atgggcttac acacttatct tgcaaaagaa
     9661 caaatggaat atggctcaga ggattcatta gactttacaa atatttactt catgctgttg
     9721 aattattgga cacttgttga aagcaacaat atcgctcgtg aaagaaaaca agttttccat
     9781 aattttgaaa aatcagctta tgctgacggc acttatttcg aaaaatacgt aactggagaa
     9841 ttccagccaa aatcagaaaa agtcaaagca ttgtttgaag gcatttttat cccaactgct
     9901 gaagattgga atgctttgaa acaagcagta atgaaagatg gactgtatca tcaaaatcgc
     9961 ttggcagtag caccaaatgg ctcaatttca tatatcaacg atacgagtgc aagtattcat
    10021 cctatcactc gcatgatcga agaacgccaa gagaagaaaa tcggtaaaat ttattaccca
    10081 gcgccttatc tatccaatga gacgattccg tattacactt ctgcttatga tatggacatg
    10141 cgtaaagtaa tcgatgttta tgcaacggca caacaacacg tcgatcaagg catgagtttg
    10201 actttattca tgcgttctga aatcccagaa ggcatttatg aatggaagac aacaacgaag
    10261 caaacgacac gtgatttgaa tattttaaga cattacgctt tccacaaagg aatcaaatcc
    10321 atttattacg tacgtacctt tactgatgat gcagaagaaa tcggaagcaa tcaatgtgaa
    10381 agctgcgtga tctaatatca tagatagatt cgcttattca tattttaatt aaaggagcat
    10441 tctcatgtca gaaacatatt atgaagcaat aaactggaat gaaattgaag atatcattga
    10501 taaatccact tgggagaagc tgacagagca attttggcta gatacacgta tcccactttc
    10561 aaatgaccta gatgattggc gtacattgtc tcaattggaa aaagatacag taggacatgt
    10621 atttggcggt ttgacactgc tagatactgt ccaatctgaa agtggcatgg atcagttgcg
    10681 taaagatgtc cgcactcccc atgaagaagc cgttttgaac aatattcaat tcatggaatc
    10741 cgttcatgcc aaaagttatt cttctatttt tagtacactg aatacgaaaa aagaaatcga
    10801 agaaattttt gactggacaa atacgaatcc ttatctgcaa aaaaaagctg agcgtatcaa
    10861 tgaaatctac aaacacggta caccgctaga aaagaaaatt gccagcgtct ttttagaaac
    10921 attcttgttc tattcaggtt tttatactcc tctttattat ttaggaaaca acaaattggc
    10981 taacgtagca gagatcatca aactaatcat ccgagatgaa tctgtacatg gaacctacat
    11041 cggttacaaa ttccaattag gattcaacga gttacctgaa gcagaacaag aaacgttaaa
    11101 agactggatg tacaatcttc tttatgagct ttacgaaaac gaagaacgtt atacagaaga
    11161 attatacgat ccaatcggct ggacagaaga agtcaagaca ttcctacgct ataatgccaa
    11221 caaagcattg atgaacctag ggatggatcc actctttcca gatactgcca atgatgtcaa
    11281 cccaatcgta atgaacggga tttcaacagg cacaagcaac catgacttct tctcacaagt
    11341 cggaaacggc tatttgttag gtcatgtaga agcgatgaaa gacgacgatt atttgattgg
    11401 attggaataa ggatttattt taattcaaaa atttttcgaa aaataaaaag agtttagcgt
    11461 acagatattt taaatgtaca ctgaactctt ttttatgctt attcacttgt ttcttccagc
    11521 acttttggac tgtctaactt ttccgaaaca tgttccattc ttctcaataa atagattttc
    11581 cggatattta atattatcgt cttaatcact gcatagacag gtatgcaaat cagcatgcct
    11641 aatattcctg cgacacttcc tgctccaatc aataccaaga tgatcgtcaa aggatggata
    11701 ttcattgatt tcccaaataa caacggcttg atcaaatttc catctaattg ttggatcact
    11761 aaaatcgaca acgccatata caaagcttga aatggagaga taaacaaacc gacaatgatt
    11821 gctggtaccg ctccaataaa agggccgaca tacgggataa tattcgtgat tccgcaaatg
    11881 accgcaagca acaagctata aggctgatcg aatattctca ttgccaaaaa gctcattaca
    11941 ccaataatca aagcatctaa gatcgtactg ctgatatagg cagataatgt ttcgtttaat
    12001 tcttttaccg tttggcgtaa ctctttacga atcgctttcg gaaagaagtg agacaacgca
    12061 tcaataaatt tgtgcccatc cttgaacata aatagcaaaa taaatggaac ggtaaataac
    12121 aaaataaaaa attgcatcat aaatgaaatg atttttgaga cacttgcaga taaactgact
    12181 aatatgatat tgaacaaatt actaagagat aaatttgctg atgctaattg ttcttccaaa
    12241 ttgaaatttt taaactcttc ccgctgcgcc aattcattta accatttgct tgtttcatct
    12301 gcatacattg gcaagctctg ggtcaattga attgtctgtt ccattaactg aggcactaca
    12361 ttcatgacag ttaaaacaac taaaatgatt acgaccaaaa acgtcaataa aaaaccgaac
    12421 actctcggga tccgatgctt ttcgagaaaa acaacgattg gatcgaacat ataatagata
    12481 aaaccagcaa caagcaatgg taaaaaaatt gccgaaagca ttttcaatat tggactaaaa
    12541 atattcggca tttgtaaaat aaagtacacg ccgatgatcg tgagtactag ctccactgtc
    12601 cagaaaaata attttgattg tttaaaacgt gattgcatac atctcctcca aggtcctttg
    12661 atacttctat tctatcattt aacaaagagt tacgcgctga gattttcatt tgcttaaaaa
    12721 aaacaaaaaa aataaacata ttttaatgag tagacgagta cttaaacaaa aacacggttt
    12781 ggagtagtga caaaaattat actgcagtcc ctttttccaa ataaacggtg ttcaaaagct
    12841 tgcatcttcg taataagccc agccaaacga aaaatataag gaactatttt tatttggctg
    12901 ggcttattac ttgatgtagg acatctattt tacaacctca gatcatttat tgcgcaatac
    12961 tgactaaatg caactttgtg atatttttgt gtacggacaa ctcctcgatc acatcggcat
    13021 aagtgagccc ttttggaaga tcaatcgtat agatattttt gtacactcgg tagtcatcaa
    13081 cgaattttac gtcaaaattc acatcttcga ttacgatatt tttctcttca aaataatcgg
    13141 taagaaaatc tttggtttct tttctatgga taaattggat ttctaacttt ttagtcgtag
    13201 gtacatggat cacgcgtttc actactgtga gtgcaatagc aatcccgata aaactgatga
    13261 tcgcaatgga atagtaaccc atgccgatgg cgatccctaa tccagcaatt gcccataaag
    13321 aagcggcagt cgtaagtccc gtcaccgatt gtttcgtgac gatgatcgtt cccgcgccta
    13381 agaaaccgac accactcacc acttgagcaa tcagccttgc ttcatctgaa cgaatcgttc
    13441 cagcaagctg tggatactct aaagcatctc ttaatgcacc actagcgatc tcaacttgga
    13501 tcaatgcaat gatacatgct cccatacaaa ctaaaatatg cgtacgcata cctgctggac
    13561 gatttttata ttgtcgttca aaaccgattg ctccgccaaa tagcatagcg atccctaaac
    13621 gtaaaataat ctctggaatt gtcaatacca tatcattcat ctaaacaccc tccagtccaa
    13681 tatatcatgt ctgaaaggag cagacaaaaa aagtgcaatt tttacctctt acgaaaaaag
    13741 gactgtgaca aaatttttgt catagtcctt tttatgaata aacggtgttc aatcagctga
    13801 ccctcggtat tactcaacaa acggcaaaat atggggagct atttttcctt tgtcttctct
    13861 aatactcagg tcaaaacgct gatttcacaa cctctttagc ctactattga aaattacaac
    13921 ttacttgctg cttcttcatc gatgatcaca acaacattgt cgtgattttg caatacactt
    13981 gctggcaaat gattcgttac aggtccttca atcatccctt tgattgcatc agctttgctt
    14041 tcgccaaatg ccatcaagac gatttcttta cttttcataa ttgaaccgat tcccattgaa
    14101 acagcttttg ttggtacatc ttctgcttta tcgaagaatc gtttgtttgc attgattgtt
    14161 gattctgtta agtcaactac tgaagtcgtt ccgtcaaatg aagcaccagg ttcattgaaa
    14221 ccgatatgtc cattttgccc gattcctaag atttggatat cgattggatg tgcatcgatg
    14281 atttgatcat actttttgca tgctgcttcc agatcttctg cttttccatc tggtacaaac
    14341 gtttctttga atggtttttt atcaaataat tggacatcca tgaaatgacg gtagctttgt
    14401 tcatctgaac cgcctagtcc aacgtactca tctaagttta cagaagtcat ttcactgaaa
    14461 tccaaatcgc ttgaagtcat ttctttatat aaagtaactg gtgtgcttcc tgttgccaat
    14521 cctaatactt ttgcaccttt gttcatacct tctttgatga tttcaaatgc tttttttccg
    14581 ccttcttcag cgttttttac acgaatgatt tccatagtga ttgctccttt atattttggt
    14641 atagcccaat tatagcattc tttttttctt tttacaataa ttagtataga ccaaattgca
    14701 tttttttcat tttcttgaca ataaatagga atccgtgtta ggtttcataa gatggttcaa
    14761 tcaaacaatc catgttgcat gaaaaccaag caagtgattc actgtggacg ctttatgtct
    14821 gcagtttttt tatgaaaaag gagttattat gagtatactg acattaaaca acattaccca
    14881 gcaatttggc gacaaaatcc tctatgaagg tgtcgatctg caattaaatg ctggcgaaca
    14941 tcttgggctg attggacaaa acggagccgg taaaagtaca ttgattaaaa tcatcactgg
    15001 tgagatctta ccagatgacg gacagatcca ttggcaaaaa aatattcaca aaggttacct
    15061 cgatcaatat gtggaagtcg atgaaacatt aacaatcgaa gaatttctaa aaactgcgta
    15121 cgcaaaagaa tacgaaaagg aagaaaaaat tgcttttctt taccaaacat atagcgagac
    15181 cttcgatgat tccttgctgg aaaaagctgg gaaactccaa acggaattag accaaggagt
    15241 gttttatcaa atcgacacct tagttgctga gatgagcagc gggttgggga tcgatgtctt
    15301 aggtttagat tcactgttga aaaatctaag tggtggacaa cgttcaaagg tgattttagc
    15361 aaaactactt ttagaaaacc cagatgttct gatcttagat gaaccaacca atcacttaga
    15421 tgatcagcat atccaatggc tgacgcagtt tcttcaggat tttgacggtg cgtatatcgt
    15481 gatttcccat gaccaagaat ttcttgatca gatcaccacc catatcgcag acatcgagtt
    15541 tggaaagatt actaagtata ctggccatct aaaacaagca ttgaaacaaa aggaacaaaa
    15601 tcgcgaaagc tatttgcgcc aatatcatgc tcaacaaaaa caaatcgaaa agaccgaagc
    15661 ttatattcga aaatacaaag ccggttcccg atcaacgatg gcaaaaagcc gacaaaaaca
    15721 attagataaa atcgaacggc tgacgcctcc agcttcagca agtaaagctg tctttgattt
    15781 tccattctcg cctattgtca cgacattagc agtggaaaca actgatttag tcatcggata
    15841 tgatcgaccg ctacttgcac ccatcaattt gaccatccgc tttggtgaaa atattgccat
    15901 tcgaggcttc aacgggattg gaaaatctac tttattaaaa acactgatcg gtgagattga
    15961 aaaaataagt ggcgatttcc acttcccaga taacacaaaa atcaattact tttcacaaga
    16021 tctgtactgg gaaaatccat tagaaacacc actacaatat ttaagcagtc aatttccaaa
    16081 agcgactatc aaagaattac gtcgccaact agctaaagca gggttagtta atcaattagc
    16141 aagtgaacct ctcactacat taagtggtgg ggaacaaact aaggtgaaac ttgcacagtt
    16201 gacaatgaac caaggtaatt tactgattct cgatgaaccg acgaaccata tcgatcaaga
    16261 aacgaaagaa agcttacaag acgggataca gaaatttcca ggaacagtaa tcatcgtctc
    16321 tcatgaacaa gaattttatc aagatcttgt gcaacgagtc attgagattg aaccaaaata
    16381 aacgtttgat tgaatagttg aaaaaaagga gctgtgacaa aagttttgtc acaactcctt
    16441 cttctgaata aatggtggcc aattagctga ccttcggtat tagctcaaaa aacggcaaaa
    16501 tatgaggagc tatttttatt tggctattct tattacttga tgcagaacag cttttcacaa
    16561 cctcttttcg tctatttttt attttcgaga tttctctttc ctttaaaaac aactgtttga
    16621 aagaagaacg cacatccttg aaaaacaagc ccgctaaccg ttacacctaa ccagccatac
    16681 tgctgccaca ttgtcgtccc tagcaatgaa ccagccgcac ctccaataaa gtacataaac
    16741 ataaataca
//
LOCUS       DAZAID010000062        16714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000062 DAZAID010000000
VERSION     DAZAID010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.062"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..1462)
                     /locus_tag="ACG9BE_001905"
     CDS             complement(251..1462)
                     /locus_tag="ACG9BE_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3032644.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1581..2216)
                     /locus_tag="ACG9BE_001906"
     CDS             complement(1581..2216)
                     /locus_tag="ACG9BE_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFN3032645.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2352..3005)
                     /locus_tag="ACG9BE_001907"
     CDS             complement(2352..3005)
                     /locus_tag="ACG9BE_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032646.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(3108..3314)
                     /locus_tag="ACG9BE_001908"
     CDS             complement(3108..3314)
                     /locus_tag="ACG9BE_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFN3032647.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3390..4136)
                     /locus_tag="ACG9BE_001909"
     CDS             complement(3390..4136)
                     /locus_tag="ACG9BE_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFN3032648.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4296..4520
                     /locus_tag="ACG9BE_001910"
     CDS             4296..4520
                     /locus_tag="ACG9BE_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HFN3032649.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            4544..5305
                     /locus_tag="ACG9BE_001911"
     CDS             4544..5305
                     /locus_tag="ACG9BE_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFN3032650.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5317..5574
                     /locus_tag="ACG9BE_001912"
     CDS             5317..5574
                     /locus_tag="ACG9BE_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032651.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5666..5917
                     /locus_tag="ACG9BE_001913"
     CDS             5666..5917
                     /locus_tag="ACG9BE_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFN3032652.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5893..6066
                     /locus_tag="ACG9BE_001914"
     CDS             5893..6066
                     /locus_tag="ACG9BE_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032653.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(6180..6587)
                     /locus_tag="ACG9BE_001915"
     CDS             complement(6180..6587)
                     /locus_tag="ACG9BE_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032654.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6748..6972
                     /locus_tag="ACG9BE_001916"
     CDS             6748..6972
                     /locus_tag="ACG9BE_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032655.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            6982..7176
                     /locus_tag="ACG9BE_001917"
     CDS             6982..7176
                     /locus_tag="ACG9BE_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032656.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7179..8201
                     /locus_tag="ACG9BE_001918"
     CDS             7179..8201
                     /locus_tag="ACG9BE_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFN3032657.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8164..8979
                     /locus_tag="ACG9BE_001919"
     CDS             8164..8979
                     /locus_tag="ACG9BE_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFN3032658.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            8997..9779
                     /locus_tag="ACG9BE_001920"
     CDS             8997..9779
                     /locus_tag="ACG9BE_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032659.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9779..10627
                     /locus_tag="ACG9BE_001921"
     CDS             9779..10627
                     /locus_tag="ACG9BE_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3032660.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10624..10866
                     /locus_tag="ACG9BE_001922"
     CDS             10624..10866
                     /locus_tag="ACG9BE_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032661.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11009..11377
                     /locus_tag="ACG9BE_001923"
     CDS             11009..11377
                     /locus_tag="ACG9BE_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFN3032662.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11392..11553
                     /locus_tag="ACG9BE_001924"
     CDS             11392..11553
                     /locus_tag="ACG9BE_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN3032663.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11550..11876
                     /locus_tag="ACG9BE_001925"
     CDS             11550..11876
                     /locus_tag="ACG9BE_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032664.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11873..12688
                     /locus_tag="ACG9BE_001926"
     CDS             11873..12688
                     /locus_tag="ACG9BE_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFN3032665.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12690..12815
                     /locus_tag="ACG9BE_001927"
     CDS             12690..12815
                     /locus_tag="ACG9BE_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032666.1"
                     /translation="MGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDEMKF"
     gene            12827..13138
                     /locus_tag="ACG9BE_001928"
     CDS             12827..13138
                     /locus_tag="ACG9BE_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFN3032667.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13135..13380
                     /locus_tag="ACG9BE_001929"
     CDS             13135..13380
                     /locus_tag="ACG9BE_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032668.1"
                     /translation="MIKKLIQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13377..13793
                     /locus_tag="ACG9BE_001930"
     CDS             13377..13793
                     /locus_tag="ACG9BE_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFN3032669.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13790..14128
                     /locus_tag="ACG9BE_001931"
     CDS             13790..14128
                     /locus_tag="ACG9BE_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032670.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14125..14355
                     /locus_tag="ACG9BE_001932"
     CDS             14125..14355
                     /locus_tag="ACG9BE_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032671.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14362..14565
                     /locus_tag="ACG9BE_001933"
     CDS             14362..14565
                     /locus_tag="ACG9BE_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFN3032672.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14562..14858
                     /locus_tag="ACG9BE_001934"
     CDS             14562..14858
                     /locus_tag="ACG9BE_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032673.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14935..15348
                     /locus_tag="ACG9BE_001935"
     CDS             14935..15348
                     /locus_tag="ACG9BE_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFN3032674.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15361..15747
                     /locus_tag="ACG9BE_001936"
     CDS             15361..15747
                     /locus_tag="ACG9BE_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032675.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16298..16525
                     /locus_tag="ACG9BE_001937"
     CDS             16298..16525
                     /locus_tag="ACG9BE_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032676.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16522..>16714
                     /locus_tag="ACG9BE_001938"
     CDS             16522..>16714
                     /locus_tag="ACG9BE_001938"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032677.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         5664 a         2726 c         3485 g         4839 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtt
      121 cgttatttaa tggtatttta aatgaagata agcgaattta cagcattttt aaatgcttat
      181 ataccgcaaa ttatcccaag tttttgtata tcctacccga gtttataccc gaaaatataa
      241 ttttttagtg ttattttata tcttttttta ataggttttc aaacgctgta gttgctgtat
      301 ctttttcttt gcgtaagaca tggccataaa cattcattgt agtattaaaa tctcggtgtc
      361 ccaatcgttt ttgaagctct tttggagtca tattaggatc gctaagcaaa taggtagctg
      421 atgtatgtct aatgtcgtga aaacgaatgt gacgcaaatt gtgacgtttg gtgaatcgtt
      481 tgaacttctt atacagataa cttccacgtg gcaaagttcc atcttgtttt gtatcaaata
      541 taaaatagtg ttttaatttc tttatattta atttccaacg aatcttttta ttctctttga
      601 ttaattcatc taacatatca aatagataag caggaccggt tactattttt tcatcatcat
      661 catttttcaa acctggcagc aaccgaatat ctgattttcc atccacttct gaaatccgct
      721 gatgaaatct tatttcttgt ttgtcaaagt caatatcttt ttcttctaaa gctgcaattt
      781 ctccttgtct tgctcctgta ataaatgcta gaacaattaa agttttaatt tctatgcttt
      841 cctcaaatgc tgcttttagc attgcatgaa tttcttcttc cgaataaggt tcttcgatat
      901 tcttttttac gcctttttct tttggtattt ttacattttc cacaggattt tctttaattg
      961 ctccgtactc atttttagcc atcaaaaata agttgttaac ggcgtataac atacgaagct
     1021 ttgtatttct tgataaaggt ttatcagagt ttctcccagg atcttttgta ttgattcttt
     1081 gagcattaac aacaattttc tttatcatgt atggtttgat atcgattaga gggataaaat
     1141 caaatttatc aagaaatcta ttctcgatca ttcgcttata gttatgataa gactttggct
     1201 ctaaattttt ttcggcctct gaaagccata tttttctata aaattcacca aaagttatat
     1261 tgaacaaatc caacgaatca tcggtcattc cttcgaattg ctctaaccag atgttcaatt
     1321 ctttatatgc cgctctttta ctacttgctt ttatagtttt gctttttctt tttgccgagc
     1381 catcaggttt gtatcctaat acagctctta gtctatatgt ttcgttgtct acttgttcta
     1441 agtatcctgt tacttctgac ataattgaat cagctctctt tctctgatac aataggtact
     1501 ataaagaagc ctatcgtata ggtttgtttc tttcttagaa cacgctcgct ttggtcggtg
     1561 gggcgtgttt tttatactaa ttagttgtga agagcaattg aaactgtctc tgttccaaag
     1621 taacctgttt gtgcttgcaa tacattattt tttgcatttg cttgggcttc agagatatca
     1681 aaaactattt taccagtttg ttgcatatca ggatttaagc tttccataaa gaaagagtta
     1741 gtgatattac cattttcatc ttgatttgcc gacatagatg cagccgaatc agcttcgaaa
     1801 gtttttccat ctgcttttaa tttgaagaat gagctatcaa cagtcacagc cttatcacct
     1861 gcatttttta cagataagtc aactactaag aaagtgtctt ttgcgttagt aggaagtaca
     1921 gatgggccaa cttgttttac tacttcaacg gagtttactt tgtattccat tttccctaca
     1981 gcaacgtcat caccgatttt ataagtcttt tcttcttttg ttgtagtttc ttttgtagtt
     2041 tctttattag tacttgttga tgttgcagta gctgaatctt tgttttcacc gccacttaga
     2101 gcaccaccaa taccaaaaaa caaaagtact attaataccc aaaaccatac ccgtttataa
     2161 aacggttttt taaccttata cattttccca tcttgaccca taactttttt tgccatttaa
     2221 atattcctca tttcttgtta taatatattt gtgatctcag aaatgaggta tgagtccgtg
     2281 ttgcagcacg ggctttttta tttataactt ttttagagat tataggcaaa atagtagggc
     2341 ataaaaatat attattgaat tccgtattta aaaaatccta attgaacttc accggaagtc
     2401 ttttgctgtg ttgtacgcaa tgcttcttct tcagacattc cattctgtac tttccatgca
     2461 acaggcgaca tcccgtattt gttaacaaaa tcagtaagtg ataaagtgtc agcgtcttgc
     2521 tgagcgcttg tttgttggtc ttctggattt tgttgagatg ctgcttgttg ttctttctga
     2581 tcttgactga taatattgcc agcatcatct gtagtcaatc cattttcata aagggccacg
     2641 ccgaaagctt cccactcttt gttggaccaa tttgcacgat cagctggagt tgactgtaaa
     2701 gtgcgttgtt tcatctgttc atatgtttct tcttgaggtg cggtttggat tgtactctga
     2761 ctggagctta taactgttgg gctaggttcc gccgtaactt ggttggagct tgtaactgtt
     2821 gaactagaat ctgttttaga tgtagatttg ctagtagatg aactggtttc agttgtttct
     2881 tttgttttac ttatttttgt ttcttggtta gaagtggcat ctgttgattc agctttttta
     2941 ttatttgaac aagctgaaag tagcagagca gtacttaaca acaacataac gctaactttt
     3001 ttcattttat actcctcttt tctttgatac aataagttgt aaagaagcct actataggtt
     3061 taacatccca cgtccacaaa ctttggtcgg tgggcgtgtt tttttgttta tagactttcg
     3121 aaaactaaag tagcttggat tctatcgcca ccaccgaaac ctttactacc accgtttgta
     3181 gtggaaatag tgtgtaatcg ataaccttta gaacactgtc tatttatagt gttttcaagt
     3241 tctgataggt tttgcgaccc ttttcctata aacttttctt ttaatacaac ttgcaataca
     3301 acgtaacttg gcatattctt ttccttcttt ctactatgat aataaatact aacccctagc
     3361 cggagcgcca actacgactg gggtattttt tagaatacga ctttcccgat tattctaatc
     3421 tgttccggtg agacgactct atcttcatat aattcgttta tagagcgtag aataatcgtt
     3481 tgactctcat aatccttata tacttcttta cacgtcacac cgtcaccatc tatttcaacg
     3541 atagcaatct caccgtcttc cacgtctggc tgttccttaa caaatacaaa ttgtccattc
     3601 ctgataactg gttccattga atcgcctata ataggaacag caaaatcagc attgtttgga
     3661 actctttcaa attcttcttc ctctacaact gtatcgccat aaactaattc agtcgggttg
     3721 gcggcagaag ctgaattttt gattgtcgga agtttttcct ttttaggaaa gtgcaccacg
     3781 tttcctcgat tttgctcttc gagttgctgt tcagcaaagt tgtagacttt tgtttgtctt
     3841 ggtttttcta attgattgta gattgtttca atggatggaa tgttattttt cttatcttct
     3901 ttaggaacat cgtaaccaag caaccatgcc tcactcacat ttaaagtttt agccaacaaa
     3961 taaagcttgt gttggtcggg cgatgactta ccattgacat attgcgatag atgacttttt
     4021 gacattttta ttccagtttc tttttgcaaa ggtaaagaca tattcaaaat atcaacttgc
     4081 tttaaatttc tttcagacat taactgatta agtcgttgct gagtcgtttg cttcatctta
     4141 tacctccttt ctgatactta ttatatacct tattgaacat aagttcaata aaaaagttaa
     4201 attaatttga acttttatgt tgacaatgtt ttatgcagga gttatacttt ggttacaaag
     4261 ttcaaaacat ttgaactaga aaggagcgaa ctaatatgag ttttgattat tctaagttgt
     4321 cgggaagaat tgttgagaaa tatggaacgc aatacaattt ttcaatcgct ttaggtttgt
     4381 cggaaagaag tttgtcatta aagttgaata accgagtacc atggaaaagc accgaacttc
     4441 aaaaagccat tgaattgcta gatattccaa aagaagaaat tggcgaatat ttttttaatt
     4501 tagaagttca aaatgtctga acttaagaaa ggaattcgaa aaaatgaaca cacaaaaatt
     4561 tgaaaacaat ctatttgaat tagaagttaa gacggaaaat ggtgaatcgt tatttaatgc
     4621 tgaaacagta gcaagaagtt tgggtttcgt ggaaacaaaa aacggtaaag aatatgttcg
     4681 gtggagaact gtcaatggat atttaaaaaa atacctttcg caagaagttg cgaaaaacga
     4741 ctttatcagt gagccaatgg tttacaaatt agcctttaaa gcaaacaacg tacttgctga
     4801 aaaattccaa gactggttag caacggaagt cctacctcaa atccgaaaac acggcatgta
     4861 cgcaacagat gaactactta acaatccaga cttgctgatt gaagtagcaa ccaaactcaa
     4921 agaagaacgc acgttacgat tgatagctga acaacgagta aatgaattac aaccgaaggc
     4981 agactattac gaccgaatct tgaacaacaa aggattagtt acagttagta cgattgctaa
     5041 gaactacggt atgagcgctg tatcgtttaa taaattgcta catgaattag gtatccagtt
     5101 caatcaaagt ggcacatggc tgctatacag taagttccaa gacaaaggat acacgcatat
     5161 tgagccattt gattatgaag ataaaaacgg caatcggcaa gtaaaaacaa ggatgaaatg
     5221 gacgcaaaaa ggacacatct tcttatatga aacattgaag aagaataatt acttaccgat
     5281 gattgaacgt gaacaaacag cataggaggt aacactatga acgaggaaat gagccaaaca
     5341 atcaaggtac aaaaaatgat agatgatcta acacatgcaa ttgatagcca agccgataaa
     5401 atcatcaagg aattgcaagg acaaaaagtt tgggatgcaa aaatgctttt caagactatc
     5461 aaatttgaaa tgaatccaac aaaaagaaag ctcgccgatg tgttggaaga aaagttggct
     5521 tccgctatca acgagcaaga attactattt gaaaccgata cttttaacag ctgatgtttt
     5581 atagatgaca gtttcatccg aatctatatc attatagatt cgattattgc aatgtacgaa
     5641 ttataaatac aggaggaaat aaccaatgga gcaactagct ttagttaacc tctcagacct
     5701 taaagcttct ttggcagaat ctgagattgc aaatgaagtg tgggatacaa agcaagctgc
     5761 ggaatattta aaaaccacaa cacgcacgct gactaaagat gccgaatctg gaaagattcc
     5821 agcagcgaaa gttggcagag agtggaggtt tagtagtatc gctttgtatc agtatctaaa
     5881 aggaggaaaa acatgggtaa attcaacaga gcactagtgt tcagcgcacc cctaatcgtc
     5941 tatgctttag gactttgggg aagcagacaa gcattaatcg gcacgatcgt ttacatggtt
     6001 tggattttta tggggcttga tgaagctgag tacagagcga aaaagcccac cgagggggct
     6061 gactaaagtg tggttctgtt tattaggtgt ttatctcgtg gccgttttag taaataacta
     6121 catgagaaaa cgtggcgaat actggtatac atcctatgcg gttctagtat gtctaatgct
     6181 tacaattttt ctaatgattt attcaaaata ggtaatactt tgtcaatcaa ataagtttca
     6241 agagattttt gttctgcatc agtgttgccg taaccaaaac ggcttaagaa aattttcata
     6301 acctctatat cttcattttg aatatatggc aggactgcat aacctgatgc ttttacagca
     6361 gatatatttt tatcagtgtt actaccaata caaataccca cattgttgag aaatgttgag
     6421 aaagttgttt gtattgtttg ggtttttgaa tcgtgttttt gtaattcaat ttctttatcc
     6481 ttcatcgatt caacatgctt attatttata accgctgtaa tcgaagggga tataagagct
     6541 actaaagcta ggataatcga aattgtgatc gtgttatcga aactcatttt ttcaccatcc
     6601 agtttttaaa ccattatatc aaaaaggaga gaagaaataa tgcaagaatt agtaattttg
     6661 aaaaataaaa aaagacacga acgccagcaa gcatgatcgt gtccaaaaga aaaatctttt
     6721 aaaccattat aacacaggag gaaataaatg atctcagtta aagggttagg cgatgaaata
     6781 ttcgaagcaa tgatgagcaa agcacaacag gacatcaaag aaaaaatcat ttctgctgca
     6841 gcgcaaggac aaactagctg cactgttcga tcgagaggaa cgacaccaag ttttcttttg
     6901 gcgctagaag atgaaggcat tttgaatatc aaacgaagag atggcagcgt cagattgttt
     6961 tggcaatttt aggaggccgt gatgaaagat tttgattctt taggcgccag acagcaacca
     7021 ccaaatgaag ctagtcctgt tggagttgat tggcaagaaa atccgttata tccaggtgat
     7081 acttgctatc taacagaaga aggctatgta ccagtagacg caattctaga gtacgtccag
     7141 caacattatc caaagattga attaggagga atttaggaat ggcaaatgat ttaacacaaa
     7201 caacacaacg ttcattagat gagcaggtaa tcagtaatct tggacggtta caagaacaag
     7261 gtttagaaat gccaccagga tatagtccac aaaatgcgtt aaaaagtgct tttttcgaac
     7321 tcactaacaa tacaggagga aacttgttgc agatggctgc aaacaatcaa gaaatgaaaa
     7381 cctccatttc taatgcactt ttagacatgg ttattcaagg gttatctcca gcaaaaaaac
     7441 aatgctattt cattaaatac ggaaacaagg ttcagctaat gcgttcatat tttggaacaa
     7501 tggcggtcct tgatcgtgta actggagggg cagatattac gcctgtagtc gttcgacaag
     7561 gtgatgagtt tgaagttgca atggatggac caaacatggt tgtcaaaaag catgaaacaa
     7621 aattcgagaa tttagacaat gaaatcattg cagcctatgt agtcatcaag ttaacaaatg
     7681 gtaaagagac cacaacagtc atgacgaaaa agcaaatcga tcaaagttgg gctaagtcaa
     7741 aaatgaaaga ttctggaccg caaaaggaat ttccagaaga aatggctaaa cggacagtaa
     7801 tcaatcgggc agctaaaaca ttgatcaaca caagcaacga caacgattta ttagttcaag
     7861 ctgcaaaaga tacgttagaa aatgaatttg acaatgatcg gaaagatata acgccacaaa
     7921 cagaaaaagt ggctactctc gaacaaaaat tcttttcaaa caaaaagatt actgaaccaa
     7981 tacaaaaaga accagatccg attgtgattc cagatgatat ccaaaatgaa gtaactcgag
     8041 ttgctgatgt acctgcccat ccagaaatag agcaagcaca tttgatcgaa aatgaggata
     8101 tcaatccaat tcaagaagaa ttgatagata ttccagactt tggacgcgag gaaggtgtag
     8161 aagatgtctc agaatttgaa gacgacgagt accctttctg atgagaatta ttactccaat
     8221 gaagctgact ggcagtacat gtcaacatca caatataagt cctttttaaa atgtgaagcc
     8281 gcagccttag caaagttgaa aggcgattgg gcaccgacgt cggatcccaa agccttactc
     8341 gtaggaaact acgtacattc ttattttgaa tcaaaagaag tacatgaagc gttcaaagaa
     8401 gaaaataaat ccaaaatgtt ttctagtagg aagccgtatg gattgttgaa agatttccaa
     8461 attgctgaac aaatgattga acgacttaag caagaagaag cattcatgaa catttatcaa
     8521 ggtgagaaag aaacgatcgt gactggggaa ctatttggaa cgacatggaa aggcaagatt
     8581 gattgcttga atgttgagga tgagtatttt gttgatatca aaacaaccaa agacatgcat
     8641 gagcggaaat ggaacgaaaa ctacggatca agagaaacgt tcattgtcaa ttttggttac
     8701 gtacttcaaa tggcaatcta tcaagagctt cttttacagc aatacggaaa gatgttcatg
     8761 ccaatcatcg ctgcggtatc gaagcaaacg cctagtgagg cgaagttgat aacgattgat
     8821 caagataaga tgagctacga gctaatcatg ctaaaagaaa atattgaaag aattgtcaaa
     8881 gtgaaaaatg gcgaagaaag gccgatacct tgcggtacgt gcgagtattg tcgaggtaac
     8941 ctttcaatta ctggattcac tagcatggac gatctttaaa aggaggtgct ttgcgaatga
     9001 atctaggata catcaagtta taccgtaaag tgactagttc attcgtttgg accaattccg
     9061 atatgttcaa gctatggata ttgtgtttaa tgaaggctag tcatgaagat agaaaatttt
     9121 tatttaacgg tcaagaagta cgtttgacaa gcggacaatt cgtcacagga gcccatgcaa
     9181 tagcaaaaga gtacaacgaa ggagtcccga gtgacaaagc aattgcatgg cgaacgctat
     9241 ggagatggct taagaaattt gaaaatgaag aattattgac aatccaatcg aacgctagat
     9301 atagcgttat aacaataata aattggtctg actatcaatc gagtgacaag ccagtgacaa
     9361 gcgacggaca atcgagtgac aagccagtga caacaaacaa gaatgataag aatgataaga
     9421 atgataagaa taataatcct cgcaactctc gaaaaacacg agagtatgca gatgacgatc
     9481 caaataaaaa attggccatt cttttattaa aactcattcg aaagaatcgt agtctcaaag
     9541 aacccgattt gaacaaatgg gccaatacga ttcgtttgac gattgaagct gacaaacgaa
     9601 ctggaaaaga agttcaagac atgattgttt gggctactaa ccatgatttt tggtccacag
     9661 tcatttcatc gccaactagc ttaagaaaaa atttcgatac aatggtgggt caaaaaaata
     9721 aaagaaagca acaaaatatt tctaatgatg agttaccaga aacaggtgag gattggtaat
     9781 gaataaaaaa ctaagtgcaa tggctgcacc ttacggcgga ttaaaaacag cagatcataa
     9841 ttgcccgaaa tgtggtgatc cattgtatat ctggaaaaca aaaaataaag atggtactga
     9901 tcgatgcggt cctacatgta tcaataagat ttgtggttat cgagaaatgg taactaaaaa
     9961 tcaaaaagaa gctatccaaa aagcgaatga agcaatgaaa aaggacgcta tcaatcgaat
    10021 gcttaacagt tcaatgatta cagatgatgc catatggacc ttcgattttg atggatacaa
    10081 agtagttgat caggaaacag cacaaataaa agcgatggct caagaatggg ctaaaaaaat
    10141 tgtaaatggt agcacgattc atgcagtgat aaccggtaga acaggagccg gcaaaaccca
    10201 tctaggcgct gcagtgatta aagaagtcat gagggcatct aattataaaa ttgcttgttc
    10261 atttatcagt tatcgagaac ttttagaaca gttgaaattt gcaatgaatg atccagaagc
    10321 aagaaaagcc gtaacaggtt cgctgatggc tgagataaag aaatcagatt tcgtggtaat
    10381 cgacgatttg ggtgcagaac taggaagaat ggaagaaaac aaccaggcaa cgccttatga
    10441 tgttgatgtt ctcacatcgc tcacagaagc tcgtctaaac aaagctacga tattcacaac
    10501 caatttatca tcgaagcaat taaaacacgc gtacggtgag cgagtgttct ctcgtgtaat
    10561 gaatggaacg aaagggaaca tagctgtatt caaaacaacg acagacaaaa ggaggaatcc
    10621 agtttgacct ttgtagtgaa aaaaatgtgc tacttagata gccgagggcg aggagaagct
    10681 agtgtcgagt ttgctaaaca ccacacgact aaagaagagg ctgaccttgt tgcaagtgtt
    10741 tgtggcggcg aagtagtcga agttattaaa cccaaaagac gattttcaaa acctaagaca
    10801 aagccagtga aaaaagagtg ttgttgtccg aaaagtaacc aagcatggat gagaggtgca
    10861 aaatgacttg tttaaaatgc aacgatgaaa cagttatttg gtataagaca ttgcttggat
    10921 ggtcaacttg tgaaccttgt ccaatatgca atgaaaatgg acgacgttcg aaagaacgac
    10981 tcgaaagact aaagaaggag tatagcaaat ggcaacaaga agcaaatacg gaaacaaaaa
    11041 gcacgaagta gacggcatca cttttgattc tatcacggaa gcagagtatt acgatctagc
    11101 cttgtggcaa gcacaggcga atggttggaa agtgaagctc caggaaaggt ttgagctgat
    11161 gccgaagttt gaactagagg gcaagaagta tcgcaagatc gagtatattc ccgacttcac
    11221 attttacaaa agcggcaagc tggtcaaagt cgtagatgtt aaggggatgc agacaaaaga
    11281 ctttaagatc aaggcaaagt tgttctgtca tcaatatcaa gtgccgttga tattagccaa
    11341 aaaatatcgg aatacgttca aggaagagcg tttttaacga ggtggtccat catgacaaca
    11401 gaagaagtga ttcaaatgcg aattcgaagc cttcggcgtg agattgacga tctggaacga
    11461 acaaaggcag tgatggtcaa tgaaacggct agaaaggcaa tcgatttgca catagagaat
    11521 ttaagaaggg aaatccgtag attggaggaa tgagcgtgga taaggaagcg gcttggcgaa
    11581 aattaatgtt gctgattcaa gatgagaact ggcaagaaga tgaagcagtg gttgctgaag
    11641 ttcagcgtct aggaaagatt gctaacggac gtatacgaaa aaagccagac aaaagaaaac
    11701 agcgcaaagg gaaaatcgtc gttgttttac acgaaggcaa aattttgatg caaggaacag
    11761 ctagtgagct gtctgcagaa actggatata cgcgtgggac tattcgaacg tacgcttggc
    11821 gaaatcacac cgatcgaaaa gggcacgaat ataagtattt ggaggaagaa aaatgaacga
    11881 aaacaaatta atcaaattgg gtgtagcagg agcagtaatc gtaggtattg gagttatcgg
    11941 aggatttaag ttcttcgaaa aaatcgataa tggatatgtg ggtgtgcgct attcaatgaa
    12001 cggcggtatc aaagatgaag cactgacgca aggtgtgaaa tttgtaggga ttgacaaagt
    12061 gatccaatat ccaattcgct tgcaaactat ccaatcaaaa aatatttcag tatctacaag
    12121 cgacggcaaa aagacaacga ttgatatcaa atatgactac aaagttgatt caactaaagc
    12181 agcaaaaatg tacaaggaat ttgggaatat cacttcggaa gatatcgaaa gtggatggtt
    12241 aaaatctaag cttcaaaagg tcgctcgtga agtttatgcg aaatatagtc tgcttgatgt
    12301 cctttcagga gattcctcta aagttgaagc tgaggtatta acgaactttg ctaaatcagt
    12361 tgaatctaaa gggtttgaag tcgaagacgt aacacttggt gttccagatg tcgataaaga
    12421 aacacaaaaa tcaatcgatg cgatcattcg agctggtcaa gaaaatgaaa aagcgaagct
    12481 agacgcagaa actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa caaaagctgc
    12541 agaagcagag gcagaatcta atcgcaaagt cgccgaatca gtaacagaca atttgattcg
    12601 ttatgaagaa gctcaagctc gcaaaaagca tggatgggta acagtaaatg gagcagatac
    12661 tgtagttacg gatgaagcag gcaaataata tgggattctt tatggctaaa attctcttgt
    12721 tcttaggttt agtaggagca gcatatctcg tgtatgcgct cctttcccaa actgatgaca
    12781 aagaagatga caacaacgat gaaatgaaat tttaggagga gaaataatgg acgaactaat
    12841 cacaaaagta gagcagtggg ctaaagacaa gggactggat caagcagatc caaaagcaca
    12901 gtttttgaaa gtagctgagg aattcgggga aatagcttcg gcgatggcaa gaagtaatga
    12961 tgaactattt aaagatagcg taggagacgt tatcgtcacg ctgattatcc tttccatgca
    13021 aaaagggaca aacgtacaag agtgtttaga aatggcatac aacgaaatca aaggacgcac
    13081 agggaaaatg gtagatggtg tattcgtgaa gtcgagtgat ttggagggca gcaaatgata
    13141 aaaaagctca ttcaattcag catggattta tatgatatcg aatcaggagc aacactatct
    13201 gtggaatcgg accatctaat cataaatttt ggtggaaagc gccagattat tttgtgggta
    13261 gttgatgatg tactgtttcc agaaattgtt catgatttcg aagaatcaaa agcggttgag
    13321 tttgaaatag tgaaaaaagt aatggaattg attgaaaaat acgaggagga cagcgaatga
    13381 taccgaagtt tagagcgtgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc
    13441 attttactaa aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga
    13501 aatcatatca cgtgcgaaac atcgacctca tgcaatccac aggaatgaaa gataagaatg
    13561 gtgtggatat atttgaaggg gatatagtat tagtcagcgt gcgaaatggc ttcgattact
    13621 tagataataa agtttgtatt gtcaaaaatt caatagatta ttccggatta gtttgtgcca
    13681 ctgttgatga agacttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt
    13741 atgaagtcat cggaaatata tacgagaata gcgagttatt ggaggtagag tgattatctt
    13801 agctactgac tacatcgatt ctttaaaaga tgaaaatggg aatgtcccag cattgctaaa
    13861 ttgtggaatt aaaagcaaga atagaatcat tttgttacgg atatcggctg atttagcaaa
    13921 atatctttat ctgagcaaga cagcagtata ttacactgcg gctacacgtt atcagtacaa
    13981 gggaaaaaca atcagtcagg attattatga tcgtttaatc agtcctgata tgtatgggaa
    14041 ttcagaatca gcaattaagc tgtttggcgc aatagagata tatgccgatg attttccaaa
    14101 tttatggttg aaggaggaag cggaatgaaa tacgaaatac cactaagtga agcagacgtc
    14161 cagtcaatta ttaacggtcg ggaggttaat aaaaaacttc ctgatggtac tgaattagtc
    14221 atcagacaaa gttatttgaa agatatggca gctccagtat taattgatcg ttttaacgtg
    14281 actgattctg tggtagagaa ccacttgaaa gaatttcgat caagtataaa cgacactttc
    14341 agattaggga gttgattgac aatgaacacc agacaccgca gagtagcaaa actaagaaaa
    14401 caggaactga atgtactaaa gacaaagttt gaaaaagaat atggaatttc agcagaagaa
    14461 acatataaag tggcaagtca gtgtgttgct gatgcaagtg atgctattcg taagtttgga
    14521 atttcgatat taagtgatga tcgtaaatgg gaggaaaaag aatgaaacta aaagacggat
    14581 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaatccta
    14641 aaacacgtaa gaaaccaaaa gtcaaagtcg gtgacatggt ccgctgtgaa gcagaggagt
    14701 tcatttatcc atttcgtgga tatgtagaac acgtctataa tcactcagcg atcattcgca
    14761 ttgaaaacac gatggaatgt gataaatgga cagctaaaag caaagagaat ttagcagtag
    14821 tgagattggt ggatatggaa ctaatcaatg acaaataaaa aagccggatc gctccgacta
    14881 acataataaa acagacaagt ttattatatc acataaaagg agcagtttga attgatgcaa
    14941 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
    15001 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattatt
    15061 acggatatgc cgaaggcacc gaagcacggc aataaggcag aggatgcatt gattcagatg
    15121 atggatatag aagcggagag agacgcgatt ttagcggctt tgatggctct tagtctgatt
    15181 agtcgtcaga tactctacta cagtttttgt gtgccagaca gcttctcaaa ctacagaatt
    15241 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaaatcgga agctctaata
    15301 gagtttgcag aagcatataa acacggaaga ataattgctt ataaataggg ggaccatata
    15361 atgtggaaag attatgtatc gttgaaagag ttgaaaaaag atcttgtttt caaaagaatt
    15421 gtagaatggt cagagagtga attaatttta gaagatggaa ccaaaatgga agttgtatgt
    15481 agcgaatcag attgctgcgc atgggccgaa ggtgaattca aaaatgtaaa gcttgatgca
    15541 gtgattacag atataaagat ttttgataaa ggtaaccgtc tctataatgg tgacggacat
    15601 acctcttatg ctgaagtcgt tgtttatcat aatcgtaatg agatcgctaa agcagaatgc
    15661 acagcgaatg atgggaatgg cggctactat tatagtgttt gtgctttaaa ggtcaaagac
    15721 aaactttgta tagtgactga cgcataaaaa aatggcggtt ttttggcgga atgatggcgg
    15781 tttttagcca tttatcagtg atattatggt agtgtcgaaa gattagtgat aggtctgaga
    15841 caaaataata ataaaaggaa catcgtttta ttattgtttc acaattaagc ttcgatagac
    15901 agcaacggaa atattaagaa taaggatgtg aattttaact ccttctaaat tgttcttatt
    15961 atctatcatc cgttgctgtt tattgttaca tacagatcgc ttaggcggtc tttttatttt
    16021 ggccgaaaac ctacattttc gatggccgat tattggagga ggaatagcat gttcaaacta
    16081 tctgaaatca ttaagaagtc cgacgttgag aaattagaga tgttaaaaaa gaagttgaag
    16141 aaatagcagt ctcttcgtga ggctgtttta ttttgctcac aaaaatagac cactatcggg
    16201 taatagtggt caggaattaa atgaaaaaga tgttagggtt gttagctaag tataacatca
    16261 taacgcttac aaaacaatgc aagaaggata aattattatg agaacttact ggtatgtgtc
    16321 attaaacaat aaatatccga agccaatgaa aggacagcat aggcgtgtag tcatgtctgt
    16381 tcaaatgaag gcgaagtatt cgattgtaga aatgctaaga gaagctacac caaaagagat
    16441 tgatcagtat aagttggttc ttattggcat tggttggttt aaggatgaac atattcaaac
    16501 aaacatgaag aggtggttga gatgaagtta tattatgttg aaaaagcaaa ttgttgggtg
    16561 gttgaaggct gtctgtcaaa gtacgctaca gatattatat ctactcaatt ttcagagttg
    16621 ggattgaagg ttatcttttg tggtgagttt gtcagtgtga aagaagcggt attgccaatg
    16681 acagcaaaga gcagacgctc gcgtggttca agtt
//
LOCUS       DAZAID010000063        16282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000063 DAZAID010000000
VERSION     DAZAID010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.063"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..850)
                     /locus_tag="ACG9BE_001939"
     CDS             complement(<1..850)
                     /locus_tag="ACG9BE_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFN3032678.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLV"
     gene            complement(1046..3145)
                     /locus_tag="ACG9BE_001940"
     CDS             complement(1046..3145)
                     /locus_tag="ACG9BE_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFN3032679.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3365..4474)
                     /gene="rpoD"
                     /locus_tag="ACG9BE_001941"
     CDS             complement(3365..4474)
                     /gene="rpoD"
                     /locus_tag="ACG9BE_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFN3032680.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(4498..6339)
                     /gene="dnaG"
                     /locus_tag="ACG9BE_001942"
     CDS             complement(4498..6339)
                     /gene="dnaG"
                     /locus_tag="ACG9BE_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFN3032681.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(6521..8836)
                     /locus_tag="ACG9BE_001943"
     CDS             complement(6521..8836)
                     /locus_tag="ACG9BE_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN3032682.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9021..9881)
                     /locus_tag="ACG9BE_001944"
     CDS             complement(9021..9881)
                     /locus_tag="ACG9BE_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFN3032683.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(9940..10572)
                     /locus_tag="ACG9BE_001945"
                     /pseudo
     CDS             complement(9940..10572)
                     /locus_tag="ACG9BE_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            10739..11500
                     /locus_tag="ACG9BE_001946"
     CDS             10739..11500
                     /locus_tag="ACG9BE_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3032684.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            11526..14117
                     /gene="mprF"
                     /locus_tag="ACG9BE_001947"
     CDS             11526..14117
                     /gene="mprF"
                     /locus_tag="ACG9BE_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFN3032685.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14305..15492)
                     /gene="tuf"
                     /locus_tag="ACG9BE_001948"
     CDS             complement(14305..15492)
                     /gene="tuf"
                     /locus_tag="ACG9BE_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFN3032686.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            15860..16216
                     /locus_tag="ACG9BE_001949"
     CDS             15860..16216
                     /locus_tag="ACG9BE_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN3032687.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4452 a         3377 c         2877 g         5576 t
ORIGIN      
        1 tgactagttt tgtcacaccg ttttcttgtg taatcaatcc ttgtttcaaa agattaccta
       61 atgcgcgctt aaatgctcct ttgctgatac caaaagcttt catgatttct tctggatccg
      121 atttatctgt aaagttgatt tgctgatctg cagaacgttc taaaatggtt aaaatcattt
      181 gtgcatcatc tgatatagct tcatatgctc ttggcttcaa tgaaagattc agtacaccgt
      241 ctggtcggac accaatcact cgacctttta tctgctcgcc tagacgaggt tcattatatc
      301 gttcagaagg atggataaac ccaagataga aatcttccgt taaaacgtac gtacctgtta
      361 atttcaagcg gtatgctgtt cctatcacat ctttgttttt catttcttct gttccatatt
      421 tgcttaaaga tttgaatatt ttttcatctg ctaaactgcc ccaaatacgg tcttttgcat
      481 caactctaag ggaaatcatc aagcgatccc cttttttagg ccatagctct gtcattgtcg
      541 gcaattcatc aagagatacg acaaagtctt tatctggaag accgatatcg acaaacgcac
      601 ctaaatcttt tcgtgtagca gtaacgattc caaaagcgta atgaccgata cggcttttag
      661 gaatttgtgt agtgatccga ttttcatgtt tttgattttg gtaaccgaat ccttcaactg
      721 cttcaccgat tttgtgttca ccttctgatt tgcttaagcc aaaggtttgt ccgttttttt
      781 ggacaaagta gtttttttca ttttcatcaa taataatacc tgtaaatata gttgctaata
      841 aatcgttcat ttttttctcc ctctatagtg aaaggttttc ttaatacatt tctttgctat
      901 cataacataa tgttatctac ttgaatagct gagccttggt ttggaaaggt gaaaaaagga
      961 gtatgacaac agttgtgtca tactcccctt tttcgaataa acggtgttaa acggcaggct
     1021 cacagcctct ttcatttgcc taaaattaat tgctcccatt tcctccgaac catccagttt
     1081 tacagttgat ggttacgatg taaaatggca aagcactttc tccaatgcct ttcatcaatg
     1141 cagattttgt tgaagtggca tatagattat aatagccttc cccattttcg attctggctt
     1201 tttctaatct gtaacctcct tcaacgatat atccacgttg gatacatgta gcaatcactt
     1261 tgctttggac acctggtgcc cacgtccatg ctggattaga tgcatcggca atatcacttg
     1321 ctctggcaga tttgattggc tgattcgctg agtttgaaga ggaaggggtc gaggatgccg
     1381 cttcttgctg tgctgcttgt gcgaccgcag cttgttgagc ggcttgagct gcagctgctg
     1441 cttgagcggc ttgttttgct tgttcttcag ctgctttttt cgcttcttct gcttttttct
     1501 tttcctcttc ttcttttttc tctttatcag tgagcatctt atctacttgt ttcaattgtt
     1561 cattcaactg agcagataga tctgtatttt tcactttttc tactgcttct tttgccgtct
     1621 tgtattggtc gcgtgttgct gaatccacta cttttccgtc tttaaagact atagctgttt
     1681 tttctttagc tgtttgcaat tcagcatatt gatttttagc ttctgtttgt gcttgttgaa
     1741 tcaattttcc aaaatctgtg ttatccgttt cgattgcttg gatctcttta tctgctttca
     1801 acttgatgtc atctgctaga tgatcatcga cgatggcagg tgctatgaac cactcattca
     1861 ctttttggat atctcggatc ttagattgga tatcttggta agttgtttct aactctgaat
     1921 atcctttttc attcttaaga ctggataaat cattttttag ttttgataaa tcttgatgaa
     1981 tcatatctgc acgaataaat tgatggtgat catctgtata aaaggcagca agtttctcat
     2041 gaagttcttc catttgttgc tgtctttttg cttctgctgc tacttcggct tctacttttt
     2101 gatgatggtc ataataagcc cagcttcctc caccgagtag aagtactgct gctaaaacga
     2161 gataaggtac tttttttgat tttttctttt gcgctttatc tggatctgtt ttttctaagt
     2221 ttgattcttg atgtgttttt tcagtatctg gcacgattgt tattgatttc gcttctgctt
     2281 caaacagctt aggctggttc tcttcttttt ctatttttgc ttcaagatca gtaccctctt
     2341 tgcttacaga ttgattttca gcatcggatt cactatttat ttcatcgggc tctgttgact
     2401 ttttgtctgt gttttcgagt atttcttttt tctctgcttc cggcagttta ttcgtttttt
     2461 ccttttctaa ttgtttttca gtaaatactt cagttttttc tgtgatatcc gtttttacgt
     2521 cttttatttt tactgtttct ttacctgtgg cgttttctct atcagagtct ttttcagttt
     2581 cttgttcctt ttctggctct actttgtttt cctgtgcaat tttttcactg atcattttga
     2641 gcagctcttt cgcgtgctct tcttttgtat cttcacgatg tgctttgata taagcggata
     2701 gtatcgaatt ttcttcctca ttatcgacga tttctttcag ttcagctact cttctttttt
     2761 gttttgattc aagcgtttct tcttgattct ttcctgattc ttccgaagtc tcatgaacag
     2821 ttgctttctt ctgttcattt ttctctatat tagttacttg atcgtgagca ggtggttctt
     2881 cgtcttctgg ttctgctttt tcgaataatt ccattacaga ttctagaggt aaatctttca
     2941 attccgacca ttcgattgga tcgtttaatt ctgtatcagg aaagactgca tcttcagttg
     3001 atttatcctg catagctgca tctgtatttg tttcttcaat tgattctttt atttgctgat
     3061 cttctgcagt tatttgatga ccgcagtctg gacagatagt attctgctcc atttttttcg
     3121 atccgcaatt tggacatttt ttcatcactt agcgatcggc tcctttacta ggtttacgct
     3181 accatttaat ctatcatatt ctatacgtca cgccaatagc ttatttttag gatttactta
     3241 aaaataatga atgaatttcc ttcctataga aaaaaaagct tgagactgcg tagtctcaag
     3301 ctttttttct tttaaaataa accaaacagt tttactgatt tctcttctgg tcaatagtcc
     3361 acgcttattc taagaaatct tttaattgtt ttgaacgaga aggatggcgc aattttctta
     3421 atgctttagc ctcgatctga cgaatacgtt cccgagtaac accaaatact ttccctactt
     3481 cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg taagcgaagg acattctctt
     3541 cccgatcagt caatgtatct agtacatctt ccaattgttc ttttagtaac tcataagctg
     3601 cgtgttctgc tggacttgtt gcttcctggt cttctatgaa atcacctaag tgagagtcat
     3661 cttcttcacc gattggcgtt tcaagggaaa caggttcttg cgcgattttc aagatttctc
     3721 ggactttttc tgttggaaga tccatttccg caccgatttc ttctggcgta ggttctcttc
     3781 caaggtcttg cagcaattga cgttggatac gtatcaattt attaattgtt tcaaccatat
     3841 gaactggaat acggatcgta cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa
     3901 tccaccatgt agcataggtt gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg
     3961 ccttcatcag tcccatgttt ccttcttgga tcaagtcaag gaactgcatg cctcgtccta
     4021 cataacgttt agcgatactt actactaaac gaaggtttgc ttctgccaga cgctgtttcg
     4081 cttcttggtc gccttcttcg attttcaatg ctaacgctac ttcttcttcc gcagttaaaa
     4141 gggagacacg gccaatttct ttcagataca tacgaaccgg gtcattgatt tttacaccgg
     4201 ttggcgcaga aagatcttct gtttttgctt gttcagcttt tttctcagca ctcttcaagc
     4261 tgtgaattga tggatcacca ttttcatcca ccacactgat acctgcgtct tcaatctttt
     4321 ggatcaattt gtccatttca tctgcattca aagtgaatgg tgtagcaagc tgattggata
     4381 aatcatcata tacaacttgt cgtttgggtt tgttttcttt gatgaaagct gctactgctg
     4441 cttcatacgt ttttttatct gttccatttg ccataaaaga aggcccccct tgagtagcta
     4501 tttggcttgt tttaactgtt tcgttaaatt gatgatttca atcgtcaatt caagctcaag
     4561 ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt ttttttaatg ttatttcatc
     4621 agctaagcca gatttgctga tcacatctag caagtcttgg atttctcgtg gcgaactttc
     4681 ttctgccacc ggtaaatatt cgatttccac caccaaccgc cttaaatttt catcttgcaa
     4741 aaagtctaaa aactctgcaa ggttgaattc ataatgttgt gagacatatg catcgaataa
     4801 aaaataaact tctgcatatg catcatgaat aaactgaaat tctcgttctt ttaaaatatt
     4861 acggacagaa acttctttaa atgcacgata taatagaatc tgctctgcct tttctacttg
     4921 cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta acctgagtgt gttgactttc
     4981 ctgaggttct tgacgatttt gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat
     5041 cgtctctctg ccgatctgaa actctgttga cagttgagat agatacatat cttgttcgat
     5101 tggtgaaaca actcggctaa gttcaactag taattcttcg aggtattcga tcttgtcttt
     5161 ttcattatct aaatttttcc cttgttttaa ataacgtgat ttaaaagtaa agactgtttc
     5221 gcgcccatga gccactaatt cttggaaaga tgcttctcca tacttacgga gatattcatc
     5281 aggatcaagc ttttctggaa cgactacgac agaaagtgct aatcgactgt tttgccgaag
     5341 caattcaatc cctcgattgg ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac
     5401 caattctttc gtcagctttt ccatttgttg gatctgttga gctgttagac ttgtccccat
     5461 agaagctaat ccagttttga taccagcctg ccatgcagca atcacatcca taaacccttc
     5521 gaacaacaaa atttctccgt ttttccggat atcgcttctt gctttatcaa gattaaataa
     5581 aacttgtctt ttattgaaca gttctgtttc tggactattc aagtatttgg gctgatcttt
     5641 ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga
     5701 attacgaagt ggaaacatca ctctttgata aaaacgatca gacatacggc catcatcatg
     5761 cgtgacaaac aatccagagc gttctaacaa ttcatttgaa attgattcat tcttgaatac
     5821 ttgctgaaga aaagaacgct cattaggtgc aaaacctatg ttgaattctt caattaattc
     5881 taaagacaac ccacgacttt gcaaatattc taaagctggc tgtccagctt ttgtatgcaa
     5941 tagcatgtga tgatagattt ccgctgcttt ttcatgagca gcaatcagtt ttcccgtagc
     6001 agattcagga ttttggacag ctatagtacg ctgacgccat tgatcttcta acggtatctg
     6061 ttcgaagtct gccactttta ccactgcttc tggaaaactt agaccttcta gttcttgaag
     6121 aaaagtaaac acgtttccgc ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc
     6181 tgcaacagaa aaagacggtg tacgttcttc atgaaaagga cacagcccca agtaattttt
     6241 accagacttt ttcaactgga catattgtcc aatcacctca acgatattcg tttgactgcg
     6301 tactgtttcg ataacctctt gaggaatccg ttgagccatt ttttcacccc ctgctctatt
     6361 gatatctttt gggtttaaca gaagatattt caggagaata accggtaaaa aatcgcacta
     6421 actgaaatca gtgcgatttt aaaggacaca attatacatg tttgattgta tcatatttca
     6481 ttgctttttc aagtataaca catttctatc tcattaagca tcaatttgct actcttttca
     6541 gtaactgatt ttgaatatca gccttccagt acaatactcg aagaacaata tttacgagca
     6601 tgaatacaaa gaagtatcct acgctctctc ccaaccctaa gtttacttcc aaaattccat
     6661 ggaacagcat tgcagctaca tatatcccta cgatcgtcgt tactgctagg aagatgcgca
     6721 atgggttgaa gggataacaa gcttttacaa ctgccatgca actgatccca atcagcaagt
     6781 aatacatcaa agtagtagtt tcagcatttc cccatgcgaa tgtttttcca agtagataca
     6841 ccgcaatgat attcactact acaagcaagg catttggtaa agcattaata agtgctgtag
     6901 gcaaataacg gtaagtgatt tttcttttat cttgttcaaa agataaaaag aaggaaggat
     6961 acccttcaat cgctaaatca atcagcgtga tttggatagg gataaacgga aatccaattg
     7021 ctgtaacggc acatataatc gataaaataa atgaataaat cgttttgata aaaaagatgc
     7081 ctgagacttt cgtcacattg ttgaccactc ggcgtccttc aaacaggact tctggcaatg
     7141 tagtaaaatc agagtccaac aataccagat tcgcaatctg acgtgttgct ccatcacctt
     7201 ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa aacatcattc acaccgtcgc
     7261 ctgtcattgc aaccgttctg ccgctttctt tcaattcatt taccagcaat ttcttttgct
     7321 gcggactcac tcgtccaaaa accgtatagc gatgagcagc ttctcgcact tccatttctt
     7381 cagtaatttg agaaagatcg acatacgcat cataattcgg taatcctgca cgtctagcga
     7441 tattggagac agtaaaagga ttgtctccag aaatcacttt cagatccaca ccttcctcat
     7501 gaagataagc tagtgtttct tgagcgtttt ttcggattgg atcatcgatt tcaaacagag
     7561 caaccggctc aacaaatgct ggtttttctt cgttcaattg ctgatttgga gcgatcccca
     7621 acataagtac gcgatagcca ttcgcttggg ctgtttttag actgtccgga agccgatctt
     7681 cttccaccaa acgttcaggc gctcctacgt acacgactcc cagttctgga aattccatcg
     7741 cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt cacatcatac cgattagaaa
     7801 gctcaaaata atctctcata gcccgcatag tgatgttgtt atcactgctt tctgccagat
     7861 acgtccccat aatttctggg atggctttct cgtacagcgg atgtagtgct tctacccgct
     7921 ggatactcat tttcccttca gtaatggtcc ctgttttatc caagcataat gtatcgacat
     7981 gggccagcgt ttcaattgaa tacatgtctt gaacaaggat tcgttttttt gccagtttgg
     8041 ttacccctgt cgttaatgca atacttatta gcaataccaa accctttgga agcatgccta
     8101 acaaagctgc tgcagatgca ataacagaaa ttttgataga agcatcgcgg atgaacagtg
     8161 cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa actagtgaat ttggatactt
     8221 tgcgaatcga atctacaagt tcagaacgaa tagctttgtg agtctttgcc tcgctagtca
     8281 agcggacagc ataattatct gcacctactc ggataacttc agccatgaca gaaccgcttg
     8341 tcaaaaagct tccagaaagc aattcagctc cagtttcttt gacaatcaaa tcagattcgc
     8401 ctgtcaaaag agcttcattt gcttctgccc gtccagatat aacctgtaaa tcggctggta
     8461 tctgttctcc tgctccgatt tttaccaaat ctcctaatac cagctcttta gtagaaatcg
     8521 attgttcttt accgtcccgg atcactagta cttctgattg ggaaacaatc gataatttcc
     8581 gtacaagatt tcttgcatgt atttcttgat aaattccaat aacgatattg acgacaataa
     8641 ttgctaaata cgccatgttg gaatacgcac ctacaaataa cagacaaatc ccaataccaa
     8701 agttcagaaa attgaataac gtcaatatat tatcgctgaa tatttgtttt gtactttttg
     8761 atgcatcttc ctggtagtca ttttgctggc cagaagccat tttctgaact acctcttgct
     8821 gtgtaagtcc attcattttc tttttctcca ttttccgcca actttcaaaa ataatcacgt
     8881 gtttaagtct agcgtgttcc aagattaaaa acaacttgtt tttataaaat ttatagaaaa
     8941 acacacgaat gattttacaa aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg
     9001 tttttattat caatctatct ttacttatat tgttcctcca aacggctcat ccaccaacct
     9061 agtgctgtac cggctataaa aagaacagga caagctagta ccccagcaac tcccaatccc
     9121 gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg
     9181 aatagcttgc tatatgcctt tgagaaaatg taatccatga tttttgacaa tccaagaaca
     9241 caaagaatcc caccaattcc gatcggcaca atcacttcaa gtcgtacttc tttaaatccg
     9301 tcagccatcg ctttatacaa tcccatatac accaaaaaat tagaagggct caatcctggg
     9361 acgatcatac ccaaaccaat caatccgccg gcaatcagcc atgttccaaa attctgatcg
     9421 acttgcccgt caaataattg agcgccgtat aataaaaaca aataagccag aacaaaagta
     9481 acaaccatga tgatcacatc agaagtatct cttccttttt tccctgcttc tttccataac
     9541 gcaggaacag ttccagcgat acatccgaca aaaaaccata atatcgttgt ttcatacgca
     9601 cctaataaaa aactgactaa aaaagaaagt agaaagatgc ctgtcactcc gcctagacca
     9661 acaggcagaa aaaacaaaac attttctttg aaattttttg taaggtgggc taaaaaagaa
     9721 atcagccgct catacatgcc aaagacagct gctaatgcac caccgctgac tcctggcaag
     9781 ataaatcctg aaccgataaa cattcccttc acaaaccgta acatccagtc tttggttgtg
     9841 taccccactt cttgattttc cattttttgc cttttttcca ctatttgcgt actccttctt
     9901 tcatttaatt ttattcatga ttgaacattt cctgctattt tacttaaaga ctttttcata
     9961 ggtctctatt attttttcat tcattttgac aatatcagga taaaacatgt tacctccatt
    10021 atgatagaga tagccgccat tgtaaagcaa ggactggatc cgccaatatc ggtattgttt
    10081 cccttcctca tttcctaaaa gtgggctcag cacatcttta gaatacgctt ctgctaattc
    10141 tactgtgttt tctccgccat gattttttac ataggcaata taatctttcc caaaattata
    10201 tgcttggaca gctgtccata aatcgcatcc ttgtttcttt gcttctacta tcatttccgc
    10261 caaatatttg acaccttgat caatactgtc agctggatta tcaatgatct cagcttctcc
    10321 atgcagactt tccgagctct gcatgagatc tgtccctttt ccttttgatt cggtcaatat
    10381 tatcgccttg atcaaattct cgtagttttc catatgataa ttttttgcag ctgttgttat
    10441 ttctatttga tagctttcca ctcgttttac cgaacgataa gttcttcctc ataaaaacaa
    10501 caccgcacca ataaccgtca gaatgatcat gtaaaaaaga atacggataa tagataatcg
    10561 ttttcgcttc atttcatcac ctagttattt tgacatcttt ttttatctta ctatttcttt
    10621 atcttcttgg ctatttcttc cttgatttct gtgtacgcct taagaaaaaa tgaattttcc
    10681 tgtatttttt gacatcttac gaaaatgatc gttgtttttc agtaaaaaca attgcccaat
    10741 gactgatata atcgttatac tttacacagt aggtgattat gtgaaaaaag aaaagaaata
    10801 tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag agcggtcaag gcttccctct
    10861 ctttttactg catggcaatg acggcagcgg ccgctttttt tcagaacagg tccctgtact
    10921 cgaacgctac tataccgtgt atctagtaga tagccgagga catggtcgtt cgacaaacga
    10981 agcatctatg ttgaattttc agttaatggc cgaggatctg aatacgatca tgttgctgga
    11041 aaaaatcgat caggcagatt ttcttggatt tagtgatgga gcaaatctag cactggtatt
    11101 tgccagcagc ttccctaaaa aagttcatcg attgatttta aattctggaa acacgcttgt
    11161 taaaggcgtt cgtttttctg ccagagtcat cagtaatatt cattatgcat gggtatggtt
    11221 actctcttta tttcgcccaa gtcttcgcaa aaatctttta gttatcaaac ttttgctgca
    11281 tgacatcggg cttacagaaa atgatttaaa aaaaatcaac tcccccactt tgatcatcgt
    11341 gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat atagctaaaa ctataccaaa
    11401 agcttccttt gtgcttgtga aagaacaagg acatgaatta gctagaaaag atcctgaacg
    11461 cttcaatcga gaagttcttc aatttctttc tgaaacataa ctattaggaa tagaaggtga
    11521 tttatttgtt aaaaaatacc atacaatggc tgaaaaacca cctaggactg ttcaaaacaa
    11581 tctttttgat ttctgtcatt gtcattattg tcggtgaatt gatgtccatc ggtaaaactc
    11641 tatctatcca gcaattagga gaaacgtttg ccactatacc tgtatggaaa agcggactaa
    11701 tgctattgat tggtcttatc tctgtgttac ctatggttgg ttatgatatt attttgaatc
    11761 ggatgcttga acaaaagcag aatcctcgtt acctgtttga gacaagctgg ctgatcaata
    11821 cgatcaacaa tattgccggt ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat
    11881 atggacaaaa gaaagagagc agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt
    11941 tgatggctgg actttctatt tacagtctgc tttctttttt acttgtagtc ttcacgccag
    12001 ttgctccttt tttgaagcaa tattggatat ggttgatcgg cggcggtcta tattttcctg
    12061 tcgtcttttt atttactact ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa
    12121 ctcaaggaca gctattgcta gtttcattgt tggaatggac cggtgtcctg tttagtttcc
    12181 tttctgttgg ttatttaatg gaaatccgga ttgatctgtg gcaaacaatt ccattattca
    12241 ttgctgcttc agtgatagga atcgtgtcta tgattccagg agaaattggg agtttcgatg
    12301 taatgatgat tatcgggttg tccgctattg gtgtgccaag agaaatagtc gttgtgtgga
    12361 tcttgctcta tcgcctgttc tactacatca ttccgttttt gattggaatc gtctttttct
    12421 tcaaaaatat cggttccact tttgatcagc gttattctgg tatccctaaa caattagcaa
    12481 cagaaattgc gcataaaatc gtggttgtct tattatactt ttctggaatc atgctagtac
    12541 tttctgccac tatcccgcaa gcttttacag agtttcgctg gcttcatagt ttgaacccat
    12601 tgaaatttca ttttattatc caatttccta gtatactatt gggatttctt ttaatcatta
    12661 tgggcagagg gatcgcagca cgtgtgaaac gagcatattt gcctacaatc ttcttgattg
    12721 ttctagcttt actttatgtt ttattaagtg attttagttt cacaccggtg gttttcttgt
    12781 ctattctgct tctgatcatt cttgcttcta aaaacgaatt atttcgggaa caattgatct
    12841 attcatggga gtggcgtacg atcgatggaa tcattattgg tgcactgtcc ttgctttaca
    12901 tcgttattgg ggtatacaat ctgccggatt tccctcatcg gcgtcaccat tttatttcct
    12961 ttttcttatt tccttctgaa aagatttggt tttcgggatt gttggccatc atcgctgtta
    13021 gtttcatgat cgttctattt gttcatttcc ttcaaggaga aaagaaacag attggcgaag
    13081 cctttaatga agaaaaggcc ctgaagatcc ttacaactta tggaggaaac tccgatagtc
    13141 aattgatctt cttaaaagat aagcggatgt ttgcctatga aaaagacggt gaaccaactg
    13201 ttcttctcca attcgcttgc ttcaataata aatgtattgt aatgggtgat ccttctggta
    13261 agaaagaaga ttttccagaa gcaatcgaag cttttatcga agaaacagat cgactatgct
    13321 atttaccagt tttctacgaa acaagcgaag agattgttat gattttgcat gaattcggct
    13381 acgatttcat taagatgggg gaagaagctt atgtagacct gaatagtttt acaacttcag
    13441 ggaaaaaaat gaagggcact cgtgctgtct tgaatcggat cgaacgagaa ggatttacat
    13501 ttgatgtctt acaaccacca ttttctactg agcaaatgag tactttcaaa aatatctctg
    13561 ataattggtt gggttcacga aaggaaaaag ggttttcttt aggctttttc tctgaagatt
    13621 atctacaaag agcgcctatt gcagttgtca aaaataccca agaagaaatt gtcgcttttg
    13681 caacgatcat gcctacttac acgaacaata aagtaggaac gattgatctg atgcgatatg
    13741 attctaaaaa agcaccttct ggcagtatgg atttcttgtt cttgcatttg tttgcctata
    13801 tgaaagaaga acatatccaa tggttcaatt tagggatggc ccctctttca aatgtcggaa
    13861 cttccagaaa gagtttttta caggaaagga ttgcatactt ggtttacgag tttggttctc
    13921 atttttattc tttccatgga ttaagagaat ataaaagcaa gtacgctaca aactgggtct
    13981 cacgttatac tctatattct agagatagtt ggattgcata tgttatgatt gctttgctga
    14041 ttattgataa tgcgccagtt gagcagacac aaggtagaat aagtggtttg aaaaaatgga
    14101 tacgaagaaa gtattaaaaa ctgcataccg atagatgaac actctcatga tgaataaaag
    14161 agactgaagt aaaacgaaag ttcatgcttc tacaatctct tcttatacaa ttgaagaaca
    14221 aagagcggtc caaacagaaa tcctgtttgg accgctcttt tattttaaac atttttcatt
    14281 accaaaatat tcgagtcgcg tttcttatgc ttcgatctct gtaacgatac ctgaacctac
    14341 tgtccgtccg ccttcacgaa ttgagaatgt cgttccgttt tcaacagcga ttggatggat
    14401 caagtcaaca tcaatcgtca cgttgtcacc aggcatgacc atttctgtat cttctggcaa
    14461 tgtgattgtt cctgtaacat cagttgtgcg gaagtagaac tgtggacgat agttgttgaa
    14521 gaatggagta tgacgtccac cttcttcttt tgtcaacacg tacacttctg ctttgaattt
    14581 tgtatgtgga gtaatggaac ctggtttagc aagtacttgt ccacgttcga tatcgtcacg
    14641 ttggatcccc cgtaacaaca cgcctacgtt atccccagct tccccgtaat ctaacgtttt
    14701 acggaacatt tctacacctg ttacaactgc tttttgtgtt tcaggtttga tccctacgat
    14761 ctctacctca tcaccgacac gaacagcacc acgatcaata cgacctgagg caacagttcc
    14821 tcgacctgta attgagaaga cgtcttccac tggcaatagt aatggtttat ctgtatcacg
    14881 ttctggtgtt gggatatatt catctactgt atccatcaat tccataatag cagcttcagc
    14941 atctggatcg ccttgcaatg cttttaatgc ggaacctttg atcacaggag tgtcatcgcc
    15001 tgggaaacca tattcgctca ataactcgcg aacttccatt tctaccaaat cgatcaattc
    15061 ttcatcatcg actaaatcaa ctttgttcaa gaaaacaatc aggtatttta caccaacttg
    15121 gcgtgacaat aaaatgtgtt cacgtgtttg aggcatcgga ccgtcagttg cagaaacaac
    15181 taagattgca ccatccattt gtgctgcccc tgtaatcatg tttttcacat agtccgcgtg
    15241 tcctggtgca tcaatatgcg cgtagtggcg tttttctgtt tcatattcta cgtgtgcggt
    15301 attgatcgta atcccacgtt cacgttcttc tggagctgca tcaatactag catagtcttg
    15361 aggatttgct aggccttttt tgcctaatac tgtagtgatc gcagcagtta aagtagtttt
    15421 cccatggtca acgtgtccaa tcgtaccaat gttaacgtgt ggtttacttc tgtcataatg
    15481 ttcttttgcc ataatttaca aacctcctaa ataatataga tcactgaaaa atttcagctc
    15541 tcaccttttt attatagtct ttttttgact aaatcaaaaa gaatacgtcc gacttacttt
    15601 ttgtaagatt cgaactaagc ttattatacg tcaatattgg tcaaagtcaa ctaagagcac
    15661 tcgtctgtct tatacgcgaa aaaagtacct tttataaata aaaaaatgtg ttttttgaga
    15721 aaaacttcaa gatagtcctt aactttcttt tgaagcaata agaacaaaat gaggaaaagt
    15781 ttaaaattca gctgttgcca acaactttca atagacgaaa tcttctattt cgttaataat
    15841 aaggaaagag gtgaagtaca tgaaaaaatt aattggcgta cttgtcgttt taggcatatt
    15901 tctcggcgga agttttgcag cttatcatta tttttacggc ggcgaagctt attatacaaa
    15961 aatcacgacg aatggcgaaa tgtcagctac ccaagcagat aacggagaga aattcacgac
    16021 ttatacctac aagcaagcgg cttataataa agatggtgaa aaaaaacagg tgacgcttcg
    16081 cgaagaaaga gaacatccat taaagatgaa tgcgtatctt aaactcaaag tcaatcctcg
    16141 taaaggtgta ttaagctggg aagaaataaa ggcttctgaa gtgcctaaaa atgcggcaga
    16201 aaaaatcgat aattagtaaa tggtcttaaa attttattag ataaaaagag gctgggacag
    16261 aagcgtttaa ctccaagaaa ta
//
LOCUS       DAZAID010000064        16032 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000064 DAZAID010000000
VERSION     DAZAID010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.064"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG9BE_001950"
     CDS             226..894
                     /locus_tag="ACG9BE_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFN3032688.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACG9BE_001951"
     CDS             complement(1094..1414)
                     /locus_tag="ACG9BE_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032689.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACG9BE_001952"
     CDS             complement(1451..2833)
                     /locus_tag="ACG9BE_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN3032690.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADQANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACG9BE_001953"
     CDS             complement(2989..4176)
                     /locus_tag="ACG9BE_001953"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFN3032691.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACG9BE_001954"
     CDS             complement(4183..5214)
                     /locus_tag="ACG9BE_001954"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN3032692.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="ACG9BE_001955"
     CDS             complement(5260..6684)
                     /locus_tag="ACG9BE_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFN3032693.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="ACG9BE_001956"
     CDS             6859..7764
                     /locus_tag="ACG9BE_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFN3032694.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACG9BE_001957"
     CDS             7855..8532
                     /locus_tag="ACG9BE_001957"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN3032695.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACG9BE_001958"
     CDS             8529..8699
                     /locus_tag="ACG9BE_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032696.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACG9BE_001959"
     CDS             8813..9709
                     /locus_tag="ACG9BE_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN3032697.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACG9BE_001960"
     CDS             complement(9764..11134)
                     /locus_tag="ACG9BE_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3032698.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACG9BE_001961"
     CDS             11372..12118
                     /locus_tag="ACG9BE_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN3032699.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACG9BE_001962"
     CDS             12115..12954
                     /locus_tag="ACG9BE_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN3032700.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13904
                     /locus_tag="ACG9BE_001963"
                     /pseudo
     CDS             12955..13904
                     /locus_tag="ACG9BE_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            14045..14797
                     /locus_tag="ACG9BE_001964"
     CDS             14045..14797
                     /locus_tag="ACG9BE_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032701.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14816..15967
                     /gene="queG"
                     /locus_tag="ACG9BE_001965"
     CDS             14816..15967
                     /gene="queG"
                     /locus_tag="ACG9BE_001965"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFN3032702.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5200 a         2999 c         3230 g         4603 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttggtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaatgc agaagcatat gtggaaaaac
    13501 taaaagaatt agatacacaa gcgaaggaaa gttttgcttc tatcgaagag aacaaaaaat
    13561 tattagtaac aagtgaaggg gcattcaagt atttttcacg agcatatgat ttgccagcag
    13621 cttatatatg ggagatcaat acagaaagtc aaggtacgcc tgatcaaatg aaagcgatca
    13681 ttgatcagat aagagcaaaa gaagtaccgg ttttattcgt ggaaaccagt gtagactcaa
    13741 gaagcatgga acgggtagca aaagaaacgg gtttgaaaat ctatgataaa ctgttcactg
    13801 attccatagc aaaagaagga gaacaaggag attcttacta tcagatgatg aaatggaata
    13861 ttgaaacgat ccatgaaggt ttgtcacaaa caaaagaaag ctgaaaagtg caaggtctag
    13921 cttgaggagc agaccttgca ttttttataa gagatatgaa aaaaaattat gctgtttttc
    13981 gtcgtacaga tacctttttt gaagaaattc cgttatagtt agagtaagga aggaaggtgt
    14041 ggctatgaat tttacacgta aagataaata tacgattaaa gaagaagtct ttcgattgat
    14101 cagccagcag gaaaataccg acaaggatca agaccttcaa tttcttgaag cagaattagc
    14161 taaattattc atagcaaatc aaaatgagac aacagaacca gatgaagagc tgtacatagg
    14221 caaatggttg tttgatgaat ttcagccttt agatgaaaat accggtatcg gttattttgt
    14281 gttcctttct ccagtcggct tcacacctgg cgtattagtt aaacaaaacg acgaaataac
    14341 cgtcaaagca tggcctgcat tattttcagt catcaaagca gaaggagtaa tcaaaaagat
    14401 ccagtcggcc tattataaaa gtcaatttat tgccaacagc aaattaatga atatgtttta
    14461 taatcgtcta gtagaaattg ctaggcctgg gaaaatcccg caaacattca cgtacacaca
    14521 gcaaaaaaat ctctatgaag aagtattgta ttcccgcgat ctgtctgagt tttcgccatg
    14581 ggaagaggta aggctccatg aactgttaag tacaaaacca gtttcattga atttttttaa
    14641 tgcattagat aacaaaagaa aattaatttc aacaaattat tgggaagtat ttgctgcaag
    14701 aaaaaaatgg atggaagaaa agaagattgc cgatcagcag atccagtttg tccagctgaa
    14761 aaaagaaaaa ctatattatg ggttatacag ccgttaacag caagcgagga agaccatgac
    14821 attaaaagaa gaaatcatac aagaaagcaa acgattagga attgacaaaa tcgggttcac
    14881 cacggcggaa ccttttgatt cattaaaaga atcgttggaa gaacaaaaag cagctggcca
    14941 tacttctggc tttgaacatc caaatatcga tgagcgtctt tatccagaaa tgacatttga
    15001 tcagccgaaa tcgatcattt ccattgcact tgcgtatcct actaggatcc atgaagaagt
    15061 gcctagggat gaaaaacgcg ggcagtttgc tcgtgcttct tgggggatcg attaccatga
    15121 tatactcaga gaccgtttga accgattgat cgaattcatc caaacaagag cagaaaaatg
    15181 gcaaaaagaa gaggaatggc gtctagctcc gcaagtagat actggagaac tgatcgatgt
    15241 cgctgtagca cagcgagctg gtttaggatt tattggacga aacggattgt tgatcactga
    15301 agagttcggt tcatttgtct atcttggtga gatcgttact aatattgtat ttgagcctga
    15361 taaaccagga gaatttggtt gcggtgactg tatgcgctgc gtgactgctt gtccgacaaa
    15421 agcattgctt ggtgacggaa gaatgaatgc ccagcgatgt ttgtcttatc aaactcagac
    15481 aaaagggatg atgccggaag aatatcgtaa aaaaatgcga aatgtcattt atggatgtga
    15541 tatctgccag ttagtctgcc cttacaatca aggaaaagat tttcattttc atgaagagat
    15601 ggaaccaaaa gtagacgaaa tatatccaaa attaaagcca atgctttcga tgtctaataa
    15661 agacttcaaa cagcaatttg ggcatttagc tggttcatgg cgtgggaaga aacctttgca
    15721 gagaaacgca ttgattgctt tagctaatct aggtgatcga acggcattgc ctgagatttc
    15781 agaatgttta acagatgtcc gccctgtcat cagaggaaca gcagcttggg ctattggacg
    15841 tctaggaaca aaagaaccgg aaaaatggct tgatgtatta gatgaaatat tagctaaaga
    15901 aacagaagaa gtcgttataa acgaattgga acatgcccag aaattattgc ggagaaagtt
    15961 aaaataaaaa aatagttaac attattcaaa gtttttgtcc agtaattgtt gtaacaatta
    16021 ctggtttttt tg
//
LOCUS       DAZAID010000065        15891 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000065 DAZAID010000000
VERSION     DAZAID010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.065"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..349)
                     /locus_tag="ACG9BE_001966"
     CDS             complement(50..349)
                     /locus_tag="ACG9BE_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN3032703.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(355..585)
                     /locus_tag="ACG9BE_001967"
     CDS             complement(355..585)
                     /locus_tag="ACG9BE_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032704.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1021..1587)
                     /locus_tag="ACG9BE_001968"
     CDS             complement(1021..1587)
                     /locus_tag="ACG9BE_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3032705.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1587..2543)
                     /locus_tag="ACG9BE_001969"
     CDS             complement(1587..2543)
                     /locus_tag="ACG9BE_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFN3032706.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2543..3199)
                     /locus_tag="ACG9BE_001970"
     CDS             complement(2543..3199)
                     /locus_tag="ACG9BE_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFN3032707.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3569..5587
                     /locus_tag="ACG9BE_001971"
     CDS             3569..5587
                     /locus_tag="ACG9BE_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN3032708.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5657..5863
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6014..8428
                     /gene="leuS"
                     /locus_tag="ACG9BE_001972"
     CDS             6014..8428
                     /gene="leuS"
                     /locus_tag="ACG9BE_001972"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFN3032709.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8776..10329
                     /locus_tag="ACG9BE_001973"
     CDS             8776..10329
                     /locus_tag="ACG9BE_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFN3032710.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10710..12494)
                     /locus_tag="ACG9BE_001974"
     CDS             complement(10710..12494)
                     /locus_tag="ACG9BE_001974"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3032711.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12509..14230)
                     /locus_tag="ACG9BE_001975"
     CDS             complement(12509..14230)
                     /locus_tag="ACG9BE_001975"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3032712.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            14330..15091
                     /locus_tag="ACG9BE_001976"
     CDS             14330..15091
                     /locus_tag="ACG9BE_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN3032713.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         5182 a         2877 c         3089 g         4743 t
ORIGIN      
        1 aagtatggtg ataaaatccg ttccgggcta cctttttagg tagcctactt taatctttgt
       61 atctttctgg atcaacgaaa gtatacttta tatagtcata acgccgatga tcgcttcgag
      121 cgtctagcac atcagtcacg atatcaaaca aaaaatatac gtctttcttc attctagttt
      181 tcgcagcagg gattttaaag tagttcttat tagaatagta gagattaatt aataagctat
      241 cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct ttgacaaatc
      301 tattcgaagg atcaaattta aaccataatt gcgtctcatt aaaaagcata accattactc
      361 cgatctattt ttaaacttag tctctacctc taatatatat cgagttttta ttttgccttc
      421 agagaatact gtttcttttt ttgcagttac aggttgttta ttttcggaaa aagctaatat
      481 agctaagatt gaaacatcca tctggaattt atctttttta ctgctttgat cataaaagtc
      541 tgcatattca tcactgatat tttttctaat aaattcttcc atcataaaaa tcacctcaaa
      601 acgattatac gaacttacgt tcttgttgta aagcgatatt tgaggggcaa aaaaggggca
      661 taatttgact gatattaagt tagctattga tatatgaatg acatacaaaa atataaaatc
      721 ttttaaaaat gcttatatat caatgttttt gccattcttt tatatatctg ttatatgtca
      781 aaaaaatgtg ttcaatttca atttgtattt gaataaagag gttgtgaaaa agctgtcctg
      841 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgat ttggttggac
      901 ttattaccgc agatataagc ttttgagcac cgtgaataaa gaggttatga aatcaacgct
      961 ctaacctgag tattagagaa aaaaacgcaa aacatatcat ttatgttttg cgttttttat
     1021 ttatcttttt ttctcaatac aatataaaat aggcggctgg ttcttttggt tgataaaccg
     1081 ataattcagc acattatatg tttcctgtgg cagtttggaa caaaaagaat caaccatatc
     1141 cagttctttt tctccacctt catgaccata gtagacgaca aggatcaatc gtccgcgagg
     1201 cacaagtcgt ttcaatattt cttccatagc tgtgcgtgta gtctctggca tagtgatgat
     1261 tgctttatca ctttttggga gataccctag attgaaaatc cctgctttga gattttgttg
     1321 ttcagctatc acttctccta acgtttcgtg gccttgtaga aacaatgaga cacgttcttc
     1381 taactgacgc tgttctagcc tttcacgagt agcatcgatt gcttgttgct ggatatcaaa
     1441 actatagaca tgaccagttt ttccgatgtg ttctgctaga aaaaccgtat cgtggccatt
     1501 gcccatcgtt gcatctacga cgtgatcccc tggttgcaag atttcttgca gtaatgtatg
     1561 gctgaaatgc aaagcagttt gcaacatcag acgctcacct ttttccgtac atcatatttt
     1621 ccttggaagc tatctctgcg aagaagttct tcatcaattg cattcaagac ttcccatttt
     1681 ttcaagctcc acattggtcc aatcaaggaa tcccacggcg catcccctgt caaacgatgg
     1741 atgatgatct cttttggtat catttccagc tggtcgcaaa tgacattgac atagtcagga
     1801 cggctcatta attgaaggcg accttcatga tagtcccgca acattctcgt tttgcgcatc
     1861 agatgcatca aatggagctt gattccttgg atatctgaat ctaaaatggt gcgtctgaca
     1921 ttttctttca tcatttctaa actttcgcct ggtaatccgt tgatcaaatg cgtacaaaca
     1981 cggatattat gtttgcgtaa tttagccacg ccatctaaat aagtttgata atcatgtgct
     2041 cggttgatca gttttgaagt ttcttcaaaa gttgtttgca atccaagttc tacccataaa
     2101 tacatccgct cgttcagttc agcaaggtaa tcaactactt catctggcag acaatccggt
     2161 cgtgtaccga ctgataatcc tacgacacct ggtaaattga ctacttgctc aaatcgttct
     2221 ttgattattt caactggcgc atgggtgttt gtaaaatttt ggaaataaac gatgtattgt
     2281 tggacatgag gccatttttg atgcatcatg tcgatttctt tctggaattg gattggaaga
     2341 gggtcttcag gtgcaacgat catgtctccc gaaccagata cactgcaaaa agtacaaccg
     2401 ccatgggcaa cagtcccatc tcgattcgga cagtcaaatc ctccatcgat cggcaccttg
     2461 aaaattttcc cgccaaactc atttcgtaaa gcgtaattcc aagaatgata tcttttattt
     2521 ccttctgaat aaggaaaagt cattaaaagt ccttctttct gtaagagtta tctatttgtt
     2581 ttcttttttc tctatacact ggataagtca ggcacagcca agaaagactg agtaagtaac
     2641 cgccgatgat atcagtagga aaatgaacgc ccagataaat gcggcttatc ccgacactta
     2701 ctataataaa catcatcagc aattgaagca atgtgcgtaa ggttcttttt tgaacaaaag
     2761 ctggaagcag aaatagtaaa gaaccgtaaa gaatcatact gcctgctgca tgtccactag
     2821 ggaaactgta acttgtttca actactaagt gcgccaatga aggacgctct cgcatgaaaa
     2881 atagtttgat caccgggttg atgattccag caattacaat aaatccagaa acgaaccaaa
     2941 atgtttctgc cctttttttc ttataccaca tccataaagc cgcacaagca aataaaatga
     3001 tgacggtaag aggattaccg aatttagtca taaagagttg gtagctgttc agatcaggat
     3061 aagaagctct gacaatgcct gtaaaccact gatcgaaacc agaaagccac gacagatgga
     3121 actttacgat ccagcccaaa atgaaaaaca ggacaagaaa gaagctacca gccacctgaa
     3181 atgatgtttt atatttcata caattcctcc tttgaagcaa tgacttatta tagcattttt
     3241 ggtccagcga aaaaaacggt aaagagataa aaccaatgta tgattcctct ttgaataacc
     3301 aatattctta taaaaaaaat aactgtaatc ggaaagacat tttttggtaa tgcaaatgaa
     3361 atgttaggga aataaaatca gtgtttttcg aaatcctttt aaacaagaac gtagacgtag
     3421 gaagaacttg aaaatgaaca gaagttgttc attacggtaa attacaatat gattacaaaa
     3481 aacaacaaag ctcttaaata acttttaata aatattaaga gaagttataa ttattaataa
     3541 tagataaatt gaagttggag gatagaagat ggaaaacatt gcaagaaaag aacggagacg
     3601 tttagaacag accaaacgtt ttcgaaaagc aaaacgaggg gcagcaatcg tgggaactgc
     3661 gatggtaggc tgttctgtcg ctgccccatt agtacagccg gttcctgttc aagcggatga
     3721 aacacctgtt cagttcggcg caagaatcaa tacagcggca tttatcacag aaattgctac
     3781 ttatgctcaa ccaatcgctc aagccaacga tctatatgca tcagtgatga tcgcacaagc
     3841 agttgtagaa agcggttggg gcagtagtgc gttatctcaa gcaccttatt ataatctttt
     3901 tgggataaaa ggcagctatc aaggacaaac cgtctacatg gatacgcttg aatttttaaa
     3961 taatcaatgg gtaaataaaa aggagccttt ccgacaatat ccttcttttg ctgaatcttt
     4021 caatgacaat gcctatgtac ttagaaatac ttcatttggc aacggttatt actatgccgg
     4081 tacttggaag agcaacacga aatcctatac agatgcgaca gcttgtttga cagggcgtta
     4141 tgcgactgat cctggttacg caggcaaatt aaataatatc atcgcaactt acggattgac
     4201 ccaatatgat acaccagcta gcggaaacgc aggcggtgga gcgactacag gtggcaataa
     4261 tactggaagt gcaggtagta ctggcggttc aagcaattct ggtaatacaa actcaggttc
     4321 agcaacttcg accacttata ctgtccaatc tggtgattct gtttggggaa tctcaaataa
     4381 attcggtatc acaatggatc aattgatcca atggaacaac atccaaaaca atttcattta
     4441 tccaggacaa aaactgacaa tcaaaggtag ccaggaaaat ggctcttcga cgaataattc
     4501 cggtaacaat accaattctt caggaaatgc tggtacatca aacggaggac aaacaactgg
     4561 tgcgaaatac actgttcaat ctggtgattc tgtttggaag atctcaaatg atcacggaat
     4621 tacgatggct caattgattg aatggaacaa tatcaagaac aatttcgttt atccaggaca
     4681 acagctaatc gtatcaaatg gcggttcttc taatgctgga gcaaatactg gctcaaatac
     4741 tgggaataca agtggctcta gttcgtccag ctcaagttct tctaatacag cttcaggaac
     4801 gaaatataca gttaaagccg gagactctgt ttggagcgtc tcaaataaat acggaattac
     4861 gatggctcag ctaatccaat ggaacaatat ccaaaataac tttatctacc caggacaaca
     4921 actgactgtt tcaaacagtg gatcaacaaa cagcacgtca ggtaatacag gttcgtcaac
     4981 caatacaaat aacagcagtt cagttaatac tggcagcagt tcgtctcaag gcacttatac
     5041 cgtgaaagca ggtgaatctg tttggagcgt ggcaaataaa aacggcatca cgatggatca
     5101 gttaatcgaa tggaataata tcaaaaacaa cttcatttat ccaggacagc aattgatcgt
     5161 taaaggtgga acttcggcaa atacaaatac aggttcaact acttctgcag cgaaaccaaa
     5221 tacaccgaat acgtctgcta cgacaagtac atcatccact ggtaatacga tgtataccgt
     5281 gaaagcgggc gagtcagttt ggagcgtagc gaacaaacat catatcacga tggatcagtt
     5341 aatcgaatgg aataatatca aaaataattt catctatcca ggacagcaag tgattgtgaa
     5401 gaaaggttca tctcaatcaa caaaccaaca atcagttact ggttcgaaaa cctatactgt
     5461 gaaatctgga gaatctgttt ggagtgttgc tgacagtcat ggaattacaa tggctcaact
     5521 aatcgaatgg aacaatatta agaacaactt tatttatcca ggtcaaacgt tgattgtagc
     5581 taaataaaat agctgttcga agaaataacg attgaattcc acaaaaatat ttgttactat
     5641 aaggctactt aaatatgaca acacttgaaa aggaagtagt aaaaagactg aagtttcaga
     5701 gagcgcatgg ctggtgagaa tgtgcacgga tgcttttgaa ctcgcctttg agttgccctt
     5761 gctgaatacc cgagtaagca agcggcggtg acggccgtta caacgttcga ggccttttca
     5821 ctaaggtaaa attaggtggt accgcgtaga ttacgtccta taaaaagagc aatcttttta
     5881 taggattttt ttatttcttt tgtgaagttt gagaaaacaa agcaaaagga aataaaaata
     5941 aacagattct gccggttttt tcgttatatt tataatagaa acaagtgttt tcaatagaac
     6001 aggaggacag caaatgagtt acaatcataa agaaattgag aaaaaatggc aaaaatactg
     6061 ggcaaaaacg aatactttca acacccatga tgatcctgaa aagccaaaat tttacgcact
     6121 ggatatgttt ccctatccat ccggtcaagg gctacatgta gggcatcctg aaggttatac
     6181 ggcaacggat atcctttcac gtgtgaaaag aagtcaagga tacaacgtgc tgcatccaat
     6241 ggggtgggat gcattcggtc tgcctgctga acaatatgcg ctagatacag gaaacgatcc
     6301 agctgagttt acccaaaaaa atatcgaaac attccgacgc caaatcaatt ctttaggatt
     6361 tagttatgac tggaatcgcg aagtgaatac aactgatccg gagtattata aatggacaca
     6421 atggatcttt acgaagcttt atgaaaaagg tctagcttat gaagcagaag tagcggtaaa
     6481 ctgggtacct gaattaggaa cagttatttc aaatgaagaa gtgatcgatg ggaaaagtga
     6541 acgtggtggc tatgatgtag tcagaaaacc aatgcgccaa tggatgctga aaatcactgc
     6601 ttatgctgat cgcttattgg atgatctaga attagttgat tggccagaaa gcattaaaga
     6661 aatgcaaaga aattggatcg gtcgttcagt aggagcaaat attgaattta aagtggctgg
     6721 tacggataaa tcctataccg tatttacgac acgaccagat actctattcg gtgcaactta
     6781 ctcggttctt gcacctgaac tagatcttgt tagagaaatt acgacccctg aacaaaaagc
     6841 tgctgtagaa gcttatatcg aagagacagc taaaaaatca gatctaaaga gaacggatct
     6901 tgcaaaagaa aaaacaggag tatttactgg tgcttatgca atcaatccag ttaatggcaa
     6961 ggaaatccca atctggatcg ccgattatgt attatcttca tacggcacag gagccatcat
     7021 ggctgttcca gcacatgatg aacgtgactt tgaattcgct caaactttcg gactagaaat
     7081 tcttccagtt atcgaaggtg gagatgttga aaaagctgct tacacagaag atggtacgca
     7141 catcaactct gaatttctag acggcatgaa caaacaagaa gcaatcgata aaatgaatga
     7201 atggctagaa gaaaatggcg tagggaaaaa agaagtcagc tatcgcttac gtgactggct
     7261 attctcccgt caacgttact ggggtgaacc aatcccaatc atccattggg aggacggaac
     7321 agtcacagct gtcccagaag aagagctacc attacggttg cctaagacaa aaaacatcaa
     7381 gccaagcggt actggagaat ctccattagc aaatatcgag gagtgggtca atgtcgttga
     7441 tcctgtaaca ggtaaaaaag gccgtcgcga aacgaataca atgccacaat gggcaggaag
     7501 ctcatggtat tacctgcgtt atatcgaccc tcataacaaa aaagaactag cagattatga
     7561 aaagttgaag cgttggctac cagttgacat ctacattggt ggagcagaac atgcggtact
     7621 tcatttacta tatgcacgat tctggcataa attcttatac gatatcggtg ttgttcctac
     7681 aaaagaacca ttccaaaaac tatacaatca aggaatgatc ttaggcgaaa acaacgaaaa
     7741 aatgtcaaaa tctcgtggta atgtagtcaa tcctgatgat gtggtcgaaa cttacggtgc
     7801 agatacactg cgcatgtacg aaatgttcat ggggccatta gatgcttcga ttgcttggag
     7861 cgaaaatggc ttagaaggaa gccgtaaatt cttggatcgt gtatggcgtt tgatcgttga
     7921 tgagaacaat aaaatgcgtg accgtatcac tacattgaat gatggcaagc ttgataaagt
     7981 gtatcatcaa acagtgaaaa aagtgacaga agactatgaa aatcttcatt tcaatacagc
     8041 aatctcccaa ttgatggtct ttatcaatga agcctataaa gtggacgctc tgccttatga
     8101 atatatcgaa ggatttgttc aactactcgc accgattgct cctcacatgg gtgaagaact
     8161 atgggcaatc cttggaaatg atggtggtat ttcttacgca ccatggccaa catacgatga
     8221 agcagcactt gttgaagacg aagtagaagt tgttttccaa gtaaacggta aagttcgtgc
     8281 gaaaagcaat gttccccgag acttaggaaa agatgagcta gaaaaagcag cattagccaa
     8341 tgaaatagtg caagaatata ttgaaggcaa aacagtcaga aaagttattg cggtaccaaa
     8401 caagttagtc aatatcgttg ctaattaatt ggctctttgt caataaggac tgatgagttg
     8461 tgcaaaatca aatctgggcc agaatgaact tcattctgtc tcagattttt tgttatttca
     8521 aattgattaa ttgattgatc aaaaaggttt tgattttcat gttctcaaac acgttcaatg
     8581 tctttttctt ctgaatggtc ttgtaagaga tctctgtaat catcaaattt ttgtctatta
     8641 atcttagtat tttttgatgt ataaactacc tgaaattgag agtacatacc aaattcataa
     8701 attttttatt agtgggacta gtgattgtat ggattttgac gcataatgaa tgaagtatga
     8761 gaaaaggaga gacaaatgga tcaagaacta tttattatcg aagtattatc agctaaacaa
     8821 aaaattatcg agaaagcgaa aaaaagagcg aaaagaataa tgttcaagct tttaagctta
     8881 gtatttttct ctggtttgct ttttttcgga gctgtatcgt tcctatatcg ccatatgagt
     8941 gcagtagtcc gttcagctga agggatgcaa aaattaaata atttgtatca cttttcgtgg
     9001 ggagttggcg gaataacaat tgttgtgctt attgtttttc ttctacttat ttggctccgt
     9061 taccatcaat tgtcgcgtca tttcgatcaa tggcttagtc aaagagcaca acagttttta
     9121 cataataaac caacttctca agatgatgac tatctttatt ttgagaagac ttcgacgagt
     9181 caagcttttc ggatacaaaa aagtgattgc cagttattgc tgagatcact tgatgattta
     9241 cctatctact taggcgaaag caaatcaaaa ttcggaatga acacattcca tattttttta
     9301 ctagaaaacg aacccagatg ggaaaaaagc cagtcttcgc ctggtggata cctgacaact
     9361 aagatgagta cattagtcac tggttttttc ctcattcttt tagctggaac aatctattat
     9421 gggagataca ctcagccaaa aatgtcttct catataaatg ctgcttctca catgacaatg
     9481 aactcaacta acgatacgca tatacaaaag acaaatgaag ggttgttagc tgatcctaag
     9541 caaaaggtaa atttgacaac acaaaagata aatgatctca aacttgatac agagacgcac
     9601 tctctctata tgacaacaga tagtggtgct aattgggtat ttgtgccgat tgattcgcaa
     9661 tggttgcgtt ttggcgaata caccctgaca acggggctga ttcctgaagg ttattggatg
     9721 gacaacacat atgatatttc cgccgatttc tcatggttta tctattcttc tggggatgat
     9781 gtacgtctgc tgacttcaag agataatggt aaaacatggc aaacgtctat tatcacccaa
     9841 tcagttcagc cgatacgtta tcgaaaagcg gtattttatg gcaacgatgg tctatttatc
     9901 tgtacatcag ccagtgggat gtctgcagaa actttgtaca tttacactac tatggatggc
     9961 ggaatcactt ggataaaaag tggaagtaca atgatcaatc agccagtaca aaatgtcagc
    10021 ttcgttacaa cttctttagg ttttgtttcg acacgtgaaa gtttgtttta tacaaacaac
    10081 ggaggacgct cattcaaaga atcagttctg tcgattccgg aaaactatca gacagggggg
    10141 atagatcttt ttcaatcacc aaatgaagtt actcaaataa gcgcgaatac gttagagaca
    10201 aaattttatt tgttgaaaaa aggaccaatc gatgcaggga aaatgtttgc ctgcctcttc
    10261 cgttcaactg ataacgggga aacatggcaa ttcgttcagc aactatcgca agtagaacaa
    10321 ggagattaaa gaagagagat tgtgagcctg acgttcagca ccgagtcaca agaaacaaat
    10381 tcaaaaaaca gcttctcata tttgttgaat agtgtaactc gtgattcgga gttgacaaga
    10441 gcaacaagaa gcgattttga agtatagctt ctcgtatttt acaagatcgt gtcttattgc
    10501 cgataggttg tctcttgaac accgtttatt cgtaaaaagg gattgtgaca agtcttttgt
    10561 cactacttac cgaacgaaac tgaaggagct atccaaaaag tatcattagt attattcttt
    10621 gtgtttctag tggcaccact agtacattta aaaaagctat cgatcatgga ctaatcatac
    10681 atgatcgata gctttttaga taatgtttgt tacgctatct gaaattgact attataaaga
    10741 ctggcataaa gcgttggtga ttggagtagt tcagaatgtg ttcctttttc gacgatatcg
    10801 ccattcttca ttacaaggat catgtctgca ttttcaatcg ttgacaaacg gtgtgcaatc
    10861 acaaaacttg tcctatcttt tgtcacagct tccattgctt gctgaatcaa tgcttctgtt
    10921 cgtgtatcta cgctagaagt tgcttcatct aaaataacga caggaggatt cgctagaatg
    10981 actcgagcaa ttgtcaataa ttgttgctgt ccttgagata acgtgccgtt ttcgctagaa
    11041 atgatcgtat catatccgtc tggcagtgtt cgaataaagt gatcacattg tgcgatttca
    11101 gccgcttcga taatttgttc tcttgtggca tccattttcc cgtaggcgat attatcagct
    11161 accgttcctt caaacagcca ggtattttgt aaaaccatcc caaaagaacg acgtagttct
    11221 ccacgtgaaa gcttggtaat atcaagaccg tcgaattcga tctttccttt attcagttca
    11281 taaaaacgca tcagcagatt gactaaagtc gtttttcctg caccagttgg accaacgatc
    11341 gctaccgttt gttttggctt tactgaaaaa tcgacttgat tcatcaatat tttatctgat
    11401 gtgtaaccaa actgtacatt tttgaaagct atttttcctt tcggttgttc taaaagtctt
    11461 gcatctggaa ggtctggaat ttcatctgtt tcatctaata tctcaaatat cctatcgatt
    11521 gctgccattg cggattggat cgagttgatc acataagaag cggtagaaat tggttcagaa
    11581 atctgattga tgtactgaag ataagcttgt aagaagccga tcgtcacacc acctgataga
    11641 acaagcatag caccgaatac cgcacttatg atgaaggcga gttgattaat gaaacgaatg
    11701 gccggataga tagcacagtt cataaattga gcacgtttaa aagcgttgta ttgcttttta
    11761 tttacttcgt gtatagcagc ttctgcatga gcttgctgat tgaatgtttt gaccactaga
    11821 ttaccagcta agatttcttc cattcgatta tttaaaacac ccagttcata ctgattctgt
    11881 tcagaaatct gtttgttttt gttcgcaatc ttagttgtaa caattgaact gccaacgatc
    11941 agcaataaga cgagcaaagt aagcttgctt tcgatataaa acagcatcag accagcagca
    12001 gctattgtta cgacagaagt aaaaaattga ttgatacctg tcaataacac ttgagaaagt
    12061 tgattgagac cggtagttgt ccgactaatg atgtcaccta cttgatgatt gtcaaaaaac
    12121 gacatcggta atgttttgaa tttttttgtg atttcttttc gcaccctcaa tgtgatcttt
    12181 tcgcttaacg ctgccattgt ccgttcctga ataaatgaag tgatgctgct taagactgaa
    12241 cagccgatca gcaataagac agggaacaac aatgcttgtt tgatatcact gaaagaaagc
    12301 tgtccaatac ctgtttttct tatttctgat aagagatcat cgatcccgat tcccatgatc
    12361 aagggcatgg ctacgactaa taggttcccg aataaactgc atagaaacaa tccgtaaaaa
    12421 tatttatgtt caggtttgat cattttccaa aaacgtatca aagcacgcgt gttcaacgtt
    12481 tttttctttt ccatcgtatc ccttctttct atattccttg tgattttgca aactcctgat
    12541 aagtttggca attagacagt agttcttcat gagtaccttg tccaacgatt tttccttcat
    12601 ctaaaacaat gatgttatct gcttgttgga ttgtactgag gcgttgagca acaatcacga
    12661 gagttttctc cgacatgtgt ttatgaaggg ctgctcttaa tgctgcatct gttttatagt
    12721 ctaaagcgga gaaactatca tcgaatacat agatctctgc tggtttgatc aacgctcgag
    12781 caatacatat ccgctgtttc tggccccctg aataattaga accgccttga gcaacaaagc
    12841 tgtccaatcc gtcaggaagg gtgtccacta gttcttctaa ttgagcgatc ttgatcgctt
    12901 tagacaactc ttctgttgag gcatattcat ttcccatctg caagttgctt aaaattgtcc
    12961 cgctgaataa gaaagccttc tgagggacat aactgatgtg ggagcggatc tgttcttgag
    13021 tcatttgagt aagaggaata ccaccaaggg ttacttttcc tatcgtaggg tcactaagtc
    13081 tcaagagaag tttcgccaaa ctactttttc cagatccggt cccgccaacg atcgctgttg
    13141 tttttccttt aggtattgtg aaatcaatat cttctagaac tggatcagag atttcagtat
    13201 atttaaatgt gacatgttca aaagcagctg catttgtctg atcaaaggtt ggttcagcta
    13261 ctgttttgat cgtctcttcc tcaatttcga tctctgcttg cagtatttct tctatccgtc
    13321 gtaatgaggc aaacgattta gggataatga ccaataccat agctgcgatc atcaagtaag
    13381 caagtgtcag catagagtat tcgactacag cagtcacgac accgatttgt aatgctcctt
    13441 ctgctacaag atttccgcca aaccacaaaa tagaagcata aacgattccc atgagcaaaa
    13501 aagcaattgg agtgacccaa gcaaatattc ggttcacttt gatcatttga ttagcatagt
    13561 cagcaaagct cttattcgtt cgcttttctt catatgattg attatcaaag gcacggatca
    13621 tattaatccc agtaaagaat tctctcaatg tgatggtgat ccgatccatt tttggctgga
    13681 tactcaaaga taaaggtgcc cctcgtttca tcagtcgata aacaatccat acataaaaaa
    13741 caacagagat caatgtaacc catgttagta atagagaata acgaaacatc atgagaatgg
    13801 tgaaaacgca aataaccggt gctggtaaga tcatttggca aaataaaaca atcatttgct
    13861 ggacattatc gacatcattc gtcattcttg tcaaaagaga tccagaacta aaacgactga
    13921 tatccttcat tgacagctgt tggaatttac ggaaatattt ctcccgcgtt tggaatccaa
    13981 acctagcgga agccaatgct gaaaagtaac tagaggcaac tgcagcgatt gttcctagaa
    14041 acgctacaag cagcatttca actccaattt tttttataac tgaggcttcg ccacttgcta
    14101 tacctgtgtc gatcagcttt gctactaatt ttggtacacc taatgtaccg gcgatttcta
    14161 aacagatcat cacaaaggta aggagagcga gtcctttgtt tttctgtaag aatgttttta
    14221 agatagacat attattcctc cttttttcct gatacttttc gattctatac cttcaagtaa
    14281 cttgaaggtc aatagaaaaa agaataaact gaaaaagagg tgagaaaaaa tgaaaaaaat
    14341 gtatacgatt ggccaagtat cggaactatt tgatttgcca aaatcaacga tccgctattg
    14401 ggatgaacaa ggattaatcc gttcaagtag acaagaagag aatgattatc gattgtttga
    14461 tatcgatgat atttttatgc tttatgatat cgttttttat agaaaattag atataccgat
    14521 caaacagatg aagaatttat atggaaaaac attgacggag ttatatgaga cacttgatga
    14581 aacagaaaga agaatccata aagaactggt ggtgatgaaa caaaaacaaa aagagataag
    14641 ggagcaaaaa aaacagttga aattgatgat cgatacaaac gaggaagaat ttcctgtaga
    14701 agagatccct tttgactgta tgatctcaac ggagtttgaa gatattgtgg aaatcaaaaa
    14761 atttctacct aactattcaa gctttgggat gatgagtatg ccagcttcca gttcacagac
    14821 gatgtatgga ttttttatcg accctagtga agtacattta tttactcaag acgtgatttg
    14881 ggagaaaaaa gatacggctg tttatcgtcg attcttattg aaatccgaga tgaaccatgc
    14941 ggaacgtaat aatattttag aaatcagaga aagaatgtat gaaaaaggat ggaaaacagg
    15001 agaagtgatc ggccagtatc tattgacgaa tacggatgaa aacaatatca gaactgaata
    15061 ttatcatgca tggatagaaa tgaaaaaata ggaaatatgt gtttaagata actcgaaaat
    15121 gatggttgtt ttagttataa agaaaaattt tctggttcga ttacgggttt aatgtggggg
    15181 attattagtc gtggatagat agaattttag ttttttagag gaaatatgtc tttaaaaatg
    15241 gaatggttgc aattcttagc gatatatacg atgtaattgt cctcttaaaa aagtatggaa
    15301 aaataattgt attgggtgtt atgtataatt catggtgaac tgagactaat gagaagaaaa
    15361 attcatattg ttatgagtga gattgtatca gatgagaatg ataaattaaa taggtgcata
    15421 aaagcattac acttaaaata cattgttgct tacattatta caatatgtta ataaggcaaa
    15481 aataaataga tggattgaat aaacatattt tttaatcaaa tagtccctac tgtgactttc
    15541 ctcagctatc ggtgatacta ccgatagcat ttttttgctt ataaaataat tataagtatt
    15601 tagagatttt tcttctaaca ttcttcaaac aagaaaaatc acaaaggtta ttgttatttt
    15661 tatatattct ttttaagaat gattgttttt ttatagaaat tttaaaaaat aaaacacgtt
    15721 attgatggat agttagtttg ttatttggta atattataga agttgactgg tagtgattta
    15781 tttttcgtcg ttttataaaa atgttatttt ttggaaaaat tcacaaatat gtaacgaatg
    15841 tttttctgga atttgctgtg ttaaaaagca gtatgactaa tataaatact a
//
LOCUS       DAZAID010000066        15457 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000066 DAZAID010000000
VERSION     DAZAID010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.066"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..2230
                     /locus_tag="ACG9BE_001977"
     CDS             170..2230
                     /locus_tag="ACG9BE_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFN3032714.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2313..2882
                     /locus_tag="ACG9BE_001978"
     CDS             2313..2882
                     /locus_tag="ACG9BE_001978"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3032715.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3057..3863)
                     /locus_tag="ACG9BE_001979"
     CDS             complement(3057..3863)
                     /locus_tag="ACG9BE_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032716.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4001..5515
                     /locus_tag="ACG9BE_001980"
     CDS             4001..5515
                     /locus_tag="ACG9BE_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFN3032717.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5918..6901)
                     /locus_tag="ACG9BE_001981"
     CDS             complement(5918..6901)
                     /locus_tag="ACG9BE_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFN3032718.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6906..7553)
                     /locus_tag="ACG9BE_001982"
     CDS             complement(6906..7553)
                     /locus_tag="ACG9BE_001982"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFN3032719.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7638..8270)
                     /locus_tag="ACG9BE_001983"
     CDS             complement(7638..8270)
                     /locus_tag="ACG9BE_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN3032720.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8286..8837)
                     /gene="coaC"
                     /locus_tag="ACG9BE_001984"
     CDS             complement(8286..8837)
                     /gene="coaC"
                     /locus_tag="ACG9BE_001984"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFN3032721.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8834..9571)
                     /locus_tag="ACG9BE_001985"
     CDS             complement(8834..9571)
                     /locus_tag="ACG9BE_001985"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFN3032722.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9568..10557)
                     /locus_tag="ACG9BE_001986"
     CDS             complement(9568..10557)
                     /locus_tag="ACG9BE_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFN3032723.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10815..11357
                     /locus_tag="ACG9BE_001987"
     CDS             10815..11357
                     /locus_tag="ACG9BE_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFN3032724.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11374..12459
                     /locus_tag="ACG9BE_001988"
     CDS             11374..12459
                     /locus_tag="ACG9BE_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3032725.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12471..13277
                     /locus_tag="ACG9BE_001989"
     CDS             12471..13277
                     /locus_tag="ACG9BE_001989"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3032726.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13278..14105
                     /locus_tag="ACG9BE_001990"
     CDS             13278..14105
                     /locus_tag="ACG9BE_001990"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3032727.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14102..15175
                     /locus_tag="ACG9BE_001991"
     CDS             14102..15175
                     /locus_tag="ACG9BE_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3032728.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4975 a         2790 c         3141 g         4551 t
ORIGIN      
        1 aaaattaaaa agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa
       61 aaatactctc ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat
      121 gaaaattact acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa
      181 aagacgttca aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc
      241 tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt
      301 ctatatgcat gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt
      361 ttctgagacc actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa
      421 acagctactt cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca
      481 ctactggtta attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct
      541 ttctatcttt tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga
      601 tattttgaat catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt
      661 atatgaaaaa gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa
      721 agtttcgata tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat
      781 gtccaaagta atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac
      841 tatttccttg tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat
      901 ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga
      961 gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa
     1021 taaagaagaa atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt
     1081 tgtattaact tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta
     1141 tgtagaagca tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa
     1201 taaagaactt caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct
     1261 acctgctgaa ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg
     1321 gaaagtcact tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg
     1381 gtcggagatc cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg
     1441 ggaaattacc tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct
     1501 tattgttcat ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc
     1561 cttaggtaaa cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga
     1621 ggatggttat ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc
     1681 gtattcactc caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca
     1741 gatagatcaa aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa aacaacaaat
     1801 ggctcatgca gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag
     1861 aatatctaga aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc
     1921 acaagtttct ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa
     1981 agaatttgtt gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa
     2041 gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact
     2101 tcctagtccg ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt
     2161 catgcgaggt atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga
     2221 ctggttctga tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat
     2281 ttggcataat ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag
     2341 aaatctggat gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt
     2401 ttgaatttcg attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg
     2461 ttggggaatt tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt
     2521 atcctgaaga acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag
     2581 caacctgtac cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt
     2641 tagcagtgaa atcagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct
     2701 tgcaagaatt tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga
     2761 gccatcaggc gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag
     2821 acggcacacc taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat
     2881 aacgaaatgg tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat
     2941 atcttacttt tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa
     3001 gaaggggcag tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat
     3061 cgtttgattt ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga
     3121 ctatagaggt aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc
     3181 ttcatagtat ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt
     3241 ttaccatctg cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca
     3301 gtgtttccgt ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc
     3361 atatcgaatt gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact
     3421 gtcatcgaaa aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt
     3481 gtatttttat tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg
     3541 aatgcatgta gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct
     3601 aaaactgttg aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt
     3661 atctttaata tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa
     3721 agcattgtat ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg
     3781 atacgtaata aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta
     3841 aaaaaggttt tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa
     3901 gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa
     3961 aaatcactta atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg
     4021 tttattaaca gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac
     4081 agcgcagaca agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca
     4141 agtatataca gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg
     4201 tggtgcaggg atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt
     4261 ggaaaagatg cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc
     4321 agaaacaaaa tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga
     4381 aacgctaaaa ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca
     4441 ctctgctgat gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat
     4501 cactggaggg atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg
     4561 atacttagtg gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa
     4621 cgcagatgtg acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt
     4681 tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt
     4741 tggtgccagc aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac
     4801 aaatcaagaa ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac
     4861 cgttgatatg gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat
     4921 tggtgaggtt gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt
     4981 caatgaaatg gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc
     5041 agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga
     5101 ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt
     5161 accaaaagaa acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa
     5221 agatgctcaa ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta
     5281 tgcgatcaaa atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa
     5341 cactgaagta ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt
     5401 cacaggtggc ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat
     5461 ttttgggcga caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag
     5521 aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga
     5581 ttgaacactg tttagtgaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
     5641 gccaagtaaa aacagcttct tatgttttta acttggttgg acttattacc gcagatgcaa
     5701 gcttttgaac accgtttatt cggaaaaaga gcgtgggaca aaagtaaaga acacttttgt
     5761 ctcacgctct aaacacgtat aaacggcgga agcagaagaa attccttcgg aaataagctg
     5821 aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa
     5881 cgcttctgtc ccagcctctt ttttgatcag catgatttta ttcagttatc ttcaaaatga
     5941 tttttccgat gtttttgttt gcttccatat attcatgcgc ttgtttgact gcgctgaatt
     6001 ggaatgtacg gtcaatgatt ggccgaatct tgccattttc tagaaaaggc ccagcttttt
     6061 gcataaaagt ttgtgcgagc tctgctttat actgatcact tctaggtgtc aataatgtac
     6121 caagcagttg gacacgtttc ccaatcaata caccaaggtc tatctcaggc acgatggtcc
     6181 cacctagcat accaatcaag acccaacgtc catcaacggc tatagaagct aaattctttt
     6241 cccagtaaga agcgccgatg aaatccaaaa taacatcgac tccccgacct tcagtgattt
     6301 ttcgtacctc ttcttgaaag tcttgttttt tatagttgat caattcatct gcaccaagtt
     6361 ctttacaaaa ggcgagtttt tcttctgatc ctgcagtaac gatgattttc gcgtgagaaa
     6421 gctgtctagc taattggatc gctgctgttc ccacaccact tgctcctgca tggatcaaga
     6481 cagtttcgcc ttcttgtaat tttccaagcc agtaaagtgt ttgataagca gtcaaaaaaa
     6541 cttctgagat tccagcagca gaaacatagt ctagcgtatc agggagaagg attgcacgat
     6601 cggcaggcat cacagcatat tcggcataac cacctagatt gactaatccg tatacttttg
     6661 tcccaggagt aaaagtggag atatcacttt tattctcaac tacctctcca gctacttcga
     6721 ctcccaaaat agggtaggga ggtttcaaag agactgattc tcttttcata atatctgttc
     6781 gattgacagc tgctgtatgg actttgatca gtaattcgcc ttgttttggt ttaggaacag
     6841 gcacttcaat ttgttctaat acatctacag aaccaggatg cttgattgaa atggcacgca
     6901 tggatctatt cctcctttgc ccattcttgg aatgctgtat tcaactcttt gctaccttca
     6961 gaaagtacta gagtcgtacg agaaatatcc gtttcttcca ctagagcggc aagtacagca
     7021 gcaactgtag agcgagtgat cgtttttgat gccttctcat taggcttagt taattgaatg
     7081 gaagtaactt cttcatcgtt agtcaatgtg actggttgaa gaatgacaaa atctaagtta
     7141 gttcgtttca gccattcatc tgcataatgt ttagtaatat aataatctgt catggactca
     7201 ggccattttg ctggttcatc tgcataaaca gcactgacca tcagatagcg agagacatgc
     7261 gcattttcag ctgcgatcac tgttttgatt gcgccatcca agtctacttg cagaagattc
     7321 tttccttgag agccggcagc aaaaatcaat acatcgatgc ctttgaatgc ttcggtcatt
     7381 ttttccacgg accaagttaa atcaaaagag acatacgaaa cattctcttt ttttactaat
     7441 gtttgggaag caacatctct cacccctgca aaaatttcat gggaacttgg agctaaatct
     7501 tttatcaggt gtcggccaat ttgtccgttt gcacctacaa caaaaatttt catttggatc
     7561 atcctttcga cgtcaaatct ttccattcaa tagtaacacc aaaagagaaa aaaatcagag
     7621 atcggctacg atgactatca gttagtaaga gaagggtgct tgaagtatct taacaagaca
     7681 ttgcatactg ctgcagtcgc aagacctgcg acgattcctt caggtatacc acttgtaaaa
     7741 ataaccgcta gaatagctga ataaacagca tttaaatttg ttccgatgac tttggcataa
     7801 tcttgctgga ataaaaagta gatcatattc atcactaaaa tcgtattggt caatgaaccg
     7861 gaaagtccag ctaagaataa agaaacggaa tctgcttttt gtttcattag tttgcgcaga
     7921 cctttataaa cgaaataagg aaccacccca atcaagatgc gaggaataaa cgcgatcagc
     7981 aaggctttcc agcttccatg ttctgttccg atgactggga taaacggaga aaagacaaaa
     8041 gacaaaggtg tttggataac tgtgctacgg atcatgctga tcagtccaaa tgtaccgcca
     8101 agaaaagcac ctagtcttgg tcctaaaacg atcgaagcaa tgattacagg aatatgcatg
     8161 gttgttgcat tgattggacc gatagggata aaacctaaag gcgtaacagc taataaaatc
     8221 aaaatagcta aaaacatcgc agttaatgta aagttttttg tatttttcat aagtactcct
     8281 tttttctaag catttctttc attaagagtc agcatgacct gttcgatgat cgtctgaata
     8341 tcagctaagg caccttttcc aaaatctcca caagctaaaa gagattctct tggttcaatt
     8401 tcctgatagc cgacagatct caatgtttcc agattacgct gagtgatcgg gttttgatac
     8461 atattcgtgt tcatggcagg ggcaatcaat tttggtgttc ccactggaag cgccattgcg
     8521 actgttgaaa gaagatcgtc tgcaatgccg tttgctagct tgccgataat gtttgctgta
     8581 gcaggcgcga tgagaaacaa gtcagcttgt tttgctagtt cgatatgatt gattacctgc
     8641 ggatttgatt cttgcatgac gtctgtatgg actgcgcgct tcgacagaga ttgcaaggtt
     8701 aatggcgtga taaaatgtgt gctgctttta gacataatca catctacctg ggcacctaat
     8761 ttgacgaatt gacttgtaag atcagcgctt ttgtaagcag aaatacttcc agtaacgcct
     8821 agtaagatct ttttcatttt gattcctcca gtctaatttt ctcagtaatc aatgcagcaa
     8881 tctcagattt actttgagct tcttccactg ttccatcttt agataagaga tagccgtggt
     8941 gtgtctcgcc atggatttcc gtcaaatcat ttgcgaaaat aaaatcagtt ttgttattta
     9001 tcagactatt ttttgcgact gccagaagag attcctttgg tacatctact aatagtttga
     9061 aaccaatgat ttttgctttt ggctgccatt cacgtagata agaaatgatt tttggtgttt
     9121 tcttcaaaac gagcacaaga tgctcggtgt tagaagagat ctttgaaaca gtggtatctg
     9181 tctgttcatt ttccgcaaac caattgtcca gttcttcttg cgttgaagaa gtgggtaggt
     9241 tttttgccag ctgttcttct gaaaaactaa aagcaggagt aaaatcactg actgccatac
     9301 tgtggataat cgcatcgtag tgttcttttt tcgttagttc ttctaattgt aaaaataagt
     9361 ccagtgcaga ttcgatcgta taaatcgtta tattggagtg tcttttcggt ttcagtgctt
     9421 gtttcgtagt cacgtaatcg attgtggtat ccggattggc agctaaataa tcagctaatg
     9481 cttgccctaa tcgtcctgtc gaatgatttg taatagcacg tacttgatcg attttttcag
     9541 tcgttcctcc agctgtgatt agaatcttca tttatggtca cctacattta ctagtgttct
     9601 tccttggtgt gttccagcaa gtagtttaga tgctgtttca ggcaattgat ccagagtaat
     9661 ctcttggacg accagttcat ctgcaatctg caaatccgcc atctgttgcc ataaagaaag
     9721 acgttgatct attggtacat tgacagaatc aattccaatc aactggatgt ttcgcagaat
     9781 aaaaggcaag acagtcgcgt ttagtttgat tcccccagca ttgccacata aagctactgc
     9841 accgttataa gagatcaatg gaagaagact gcttagctgc tcgccaccta ctgtgtcgat
     9901 gacatagtcg atttgctgtt ttcctaaagc ttttgttgtc tctggcagaa attcatcagg
     9961 tcgtacgact tgcaaagcac ctagactttt tagccatgtg acagactctt cttttctgga
    10021 aaaggcaatg attgactgga atcctagttt atgtaacagg gctatcgctg tactgcctac
    10081 accgccagaa gcaccagtaa cgacaacttt tttatcggag gtcatacctt gacggattaa
    10141 agcgttcaca cacaacatag cagtaaaccc tgctgtacca aggatcatcg cttgacgcag
    10201 cgtcatattt tttggtagag gaactaacca gtctcctggt actttttgat attggctata
    10261 accgcctgga tgggtgacgc caagcccaaa accagtagca agtacaagat cttcttttga
    10321 aaaacgtgga ttgttacttt ggacgacagt tccggataaa tcaatccctg gagtcatcgg
    10381 atatgttcgt atcacctgac cagactcgct cattgccaat gcatctttat aattgacatc
    10441 agaataggca atttttacca agacttcatt ttcagccaac tcagtaaatg gcagatcact
    10501 gattgctgta tggaaaacag gttcctttat tacctgaaaa gatcgaaaag cgttcattcg
    10561 tgagacactc cttttaaaat tttcttttat cataccataa agcctactct tttattcttg
    10621 ttcttccgta aatttagaca aaaattaggt gaaaaataaa gaatcaaaca actagaattt
    10681 attccaaaag ataaagaaat attcagcaaa aaaaatttta caaagggttg cctttttcaa
    10741 taaatttcgt atactaatac gagtggtagt aaacaggaag tttactatta gaaaacttcg
    10801 attcggagga aattatggaa attggtgaga agttaagaaa tttgcgcatc caaaagaatt
    10861 tgacacaaga agaactaggc gaacgcacag atttgtcaaa agggtatatt tcccagttgg
    10921 aacgagattt aagttcacca tcaatggaaa cattcttttc tattcttgaa gtcttaggtg
    10981 tgactccaga agaatttttt catcaagaag cagcgaatct gcaagtggtt tattcaaaag
    11041 aagatcatac ggtttatttc gatgaagaaa acgggtatga attggaatgg ctcgtggctg
    11101 attcgaatga aaaagaaatg gagcctgtct tgctgacttt tgaaggtgca ggagaatata
    11161 agacgtttga accttcatta tctgaaacgt ttatttttgt attagaaggg gaattagagt
    11221 taagcttagg agagacggtc tatgtggcaa aagcaggaca gtcgatctat tatcaagcga
    11281 caaaacaaca tcaattaagg aatcatctca aaaaacgaac aaaagtattg attgttgcaa
    11341 cgcaatcata tttgtaatat agggggtaaa aacgtgggga aaccaatcat ttcatttgat
    11401 caagtagtga aacgctatga tgatgaaaca gtattaaaaa aagtaagttt tgagatcgaa
    11461 caaggaaaat tttatacact gcttggtcct tccggctgtg gtaaaacaac gatcctacgt
    11521 attatagctg gattcaccga agctacagaa ggggacatct attttgaagg gaaacggttg
    11581 aatgatttgc cagctaataa gcgacaagta aatacagtct tccaagatta tgcacttttt
    11641 cctcatatga atgtttttga taacgttgct tttggattga agatcaagaa aatgtccaaa
    11701 tcagatatcg aaaagaaggt aaaagatgct ttgcgtatgg tccagcttcc tggatacgaa
    11761 aaacgggaga ttagtgaaat gtctggtggt caaagacagc gtgtagcgat tgcacgagca
    11821 attgtaaatg aaccgaaagt attgctatta gatgaaccgt tatcggcgct tgatttgaaa
    11881 ctacgtacag atatgcagta tgaactgcgg gatctgcaac aacgattagg aatcacgttt
    11941 atttttgtta ctcatgacca agaagaagcg ttggcaatga gtgatgagat ttttgtcatg
    12001 aataaaggga aaatcgtcca aagtggtaca cccgtagata tttatgatga gccaatcaat
    12061 catttcgttg ctgattttgt tggggagagc aatatcgtgg acggtgtaat gatcgaagat
    12121 aatctagttg agtttgttgg taagcaattt gaatgtgtcg atggcgggat gcgtctaaat
    12181 gaaccagttg aagttgtttt acgcccagag gatttgacga tcacgacacc agataaagga
    12241 aaactagtcg taacggtcga tactcagctg tttcgtggtg tccattatga aatcatctgt
    12301 tatgatgaac agcaaaatga atggatggtc cattcaacga aaaaagctaa agaaggaagc
    12361 aaagtaggat tggcctttga gccagaagat atccatgtca tgcgtttcaa cgagtctgaa
    12421 gaagaattcg atgcacgtct tgacagctac gaagaatagg ggggacaaga atgaaaacaa
    12481 tgcgcaggat ctattccgtc ccttatattt tgtggctcgg tttattcgtg atcgcgcctg
    12541 tattaatgat catttatcaa tctttttttg atatgaatgg gcaattcacg ctgaataatt
    12601 atctatctta ttttacgtcg gggacatcgc tttctatgac gatcaattct gtatggtatg
    12661 catttttgat cacgtttttc acgctgatca tcagttaccc gactgcttat tttttaacaa
    12721 aactgaagca caagcaatta tggctgatgc tgattatttt gcctacatgg gtgaatctgc
    12781 tgcttaaagc ttacgcattt attgggattt tcagtgtgca cggcagcgtg aatcatttct
    12841 ttgattttat cgggatcggg ccgaaacaga tattattcac agacttcagt tttctgttcg
    12901 tggcaacgta tatcgagatt ccatttatga ttatgccaat tttcaatgca ttggaagaga
    12961 tgaatccttc tttgatcagt gctagtagag atttaggcgc gaatagcttt gagaccttcc
    13021 gacgagtgat ttttccgtta tcattaaatg gtgtgaaaag tggtgtccaa gcagtattta
    13081 ttccttctct ttctttattc atgctgactc gtctgattgg cggaaatcgg gtgatcactt
    13141 taggaacggc tatcgaacag catttcttgg ttacacaaaa ctgggggatg ggttctacaa
    13201 tcggtgtgat tttaatcatt gctatgttca tcgtgatgct cctgacaggc gagaagaaaa
    13261 aaggaggtcg ctcataaatg aaaaaattta aatggtcgta tctttattta gggatcgtct
    13321 ttttattcct atatgcaccg atcttctatc tgatttttta ttccttcaat gaaggaggaa
    13381 cgatgaacag ctttacagga tttacttggg aaaattatga agctgttttt gaagatacac
    13441 gtttgatcat tatcgtattg aatacgttca tgctggcttt cttatctgca ttgatcgcca
    13501 cgatcatcgg gacatttggg gctatgggaa tctattacac gaaaaaacga cgtgcacgga
    13561 cagcgttgtt gagcatgaat aacatcttat tagtctctcc tgatgttatt atcggtgcta
    13621 gtttcttgat tttctttaca ctactaggat tcggattagg ttttcagagt gtattgcttt
    13681 cccatattgc tttcagcatt ccaatcgtcg tcttgatggt cttgccaaaa ttgcaggaaa
    13741 tgaacgattc aatggtcgat gcggctagag accttggtgc taataatttc caagtagtca
    13801 aaaatatcat tttgccttat ctttctccag gaatcatagc tggatatttt atggcattta
    13861 cctattctct tgatgatttt gcggttacat ttttcgtcac aggaaatggc tttacgacgc
    13921 tctcagtaga gatctattct cgtgcaagac aaggaatcag tttagaaatc aatgcgctaa
    13981 gtgcgttagt tttcttgttc tccatgatat tggtcattgg atactacttt attagtaaag
    14041 aaaaccgtcc aaaacggctg aagaaaaaca gaagagtacg cgagggggtg gcacggctgc
    14101 gatgaaaaaa ttacaatctc tgtttttagg tattctcatc attattgcgg tgctgctgct
    14161 tggtgttcgt caacttgaaa aaacaagtgg catgagtgga gcaaaggtac ttaccatcta
    14221 taactgggga gattatatcg atcctagttt gatcacgaaa tttgaaaaag aatatggata
    14281 caaagtgaat tatgaaacat ttgattctaa tgaagcgatg ttcacaaaaa tccagcaagg
    14341 tgggacaaac tacgacatcg ctataccaag tgaatatatg atccaaaaaa tgatcaaaga
    14401 aaaactagta cttccattag atcattcgaa gatcaaagga ttaaataata ttgatccacg
    14461 atttttagac ttggattttg atccagataa tcagtattcg attccatatt tttggggaac
    14521 tttagggatc gtatataacg ataaattctt tgatgcaaat caaattaagc attgggatga
    14581 tttgtggaag ccggaattaa aagacagttt gatgctgatt gacggtgcac gagaagttat
    14641 ggggctttct ttgaatagtt taggctattc tttgaatagt aagaatatgg cacagctgac
    14701 agaagcagca aataaattga atcatttaac accaaatgtc aaagcaatcg tggcagatga
    14761 gatcaaaatg tatatgatca atgaagaagc ctccgtggca gtgacatttt ctggcgaagc
    14821 tgccgatatg atgtgggaaa atgaacattt acattatgtg atcccaccag aaggatccaa
    14881 tctttggttt gataatatcg ttatgccaaa aaccgtgaaa aataaagaag gtgcctatga
    14941 tttcatcaac tttatgctag aaccggaaaa tgcggcacaa aatgcagagt atattggtta
    15001 ttctacacca aatgaagaag caaaagcgtt attgccaaaa gacatttcgt cggacgaaca
    15061 attctatcca tctgatgaca cgatctccca tcttgaagtc tacaaagatc taggatcgaa
    15121 atacctaggc atctataatg acctattctt agaattcaag atgtatagga gataatggca
    15181 aaactcagta agaatgggga cttatcaacg ttctaaagag ctaaaaatcg tcctttttct
    15241 atctgagttt tccaatcttc taccaaaagc ggatgaaacg ccgctttttt tgtgttattt
    15301 tattttttct accacgtttt tctaccagag aaaagtggac aaaaccattg taaaataggg
    15361 cccgagacgg gctagtaaat ctaagattga aaaagttaca gaaggataaa gtaattggaa
    15421 actatgcaaa tatacttaac ccttctgtaa tctatta
//
LOCUS       DAZAID010000067        15251 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000067 DAZAID010000000
VERSION     DAZAID010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.067"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..1137)
                     /locus_tag="ACG9BE_001992"
     CDS             complement(94..1137)
                     /locus_tag="ACG9BE_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFN3032729.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1514..2479)
                     /locus_tag="ACG9BE_001993"
     CDS             complement(1514..2479)
                     /locus_tag="ACG9BE_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFN3032730.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2713..3558
                     /locus_tag="ACG9BE_001994"
     CDS             2713..3558
                     /locus_tag="ACG9BE_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN3032731.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3749..4936
                     /locus_tag="ACG9BE_001995"
     CDS             3749..4936
                     /locus_tag="ACG9BE_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFN3032732.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4942..5298
                     /locus_tag="ACG9BE_001996"
     CDS             4942..5298
                     /locus_tag="ACG9BE_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFN3032733.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5300..5638
                     /locus_tag="ACG9BE_001997"
     CDS             5300..5638
                     /locus_tag="ACG9BE_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFN3032734.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5714..5970
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6033..7067
                     /locus_tag="ACG9BE_001998"
     CDS             6033..7067
                     /locus_tag="ACG9BE_001998"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFN3032735.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7060..7746
                     /locus_tag="ACG9BE_001999"
     CDS             7060..7746
                     /locus_tag="ACG9BE_001999"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFN3032736.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7770..8618
                     /locus_tag="ACG9BE_002000"
     CDS             7770..8618
                     /locus_tag="ACG9BE_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN3032737.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8815..9588
                     /gene="sufC"
                     /locus_tag="ACG9BE_002001"
     CDS             8815..9588
                     /gene="sufC"
                     /locus_tag="ACG9BE_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFN3032738.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9601..10887
                     /gene="sufD"
                     /locus_tag="ACG9BE_002002"
     CDS             9601..10887
                     /gene="sufD"
                     /locus_tag="ACG9BE_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFN3032739.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10884..12119
                     /locus_tag="ACG9BE_002003"
     CDS             10884..12119
                     /locus_tag="ACG9BE_002003"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFN3032740.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12106..12576
                     /gene="sufU"
                     /locus_tag="ACG9BE_002004"
     CDS             12106..12576
                     /gene="sufU"
                     /locus_tag="ACG9BE_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFN3032741.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12581..13972
                     /gene="sufB"
                     /locus_tag="ACG9BE_002005"
     CDS             12581..13972
                     /gene="sufB"
                     /locus_tag="ACG9BE_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFN3032742.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14070..15212)
                     /locus_tag="ACG9BE_002006"
     CDS             complement(14070..15212)
                     /locus_tag="ACG9BE_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3032743.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4983 a         2845 c         3258 g         4165 t
ORIGIN      
        1 tgttgttgtt agaaggatat ctgcaaaatt aaaaacagtt gtgattcgga cattgttcca
       61 tgcgagaatc acaactgttt tttcatgttc attctaaact ttgatcagcc aaataaatag
      121 taaagccatc actgtttgga gaagcccgac agctaatcca taagataaga agcgtgtacc
      181 ttctttcatg aattttgcaa agtctaaacg cagaccaatt gccgctaaag cagtgatctc
      241 aaaccagctg ctgataaaat gtgccgattg gtcaataact acaggtaaag aaaacaagct
      301 gttgaccaaa caaaagatca caaaccctaa tacataccaa ggaagcggta attttcgttt
      361 tttctgcgta tcagttggcg tttcaaacag agctggttgt ttcttgtgca tgaatttttc
      421 aaacagatag acaacaacaa cgagcatcat aatacgtaga atcttaaata gcatggcgaa
      481 ttcgacaaca gaattgtcaa gtaagcttgc cccagcgact acttgaccaa cagattggag
      541 tgtaccaccc aaaagtgcac ttttttcaat gagatttcca ccaaacaact gtaatccaag
      601 aaaagggaga gtcaacatca tcacagtacc cagtagattg actagtgtga tgatctgtcc
      661 tttttcttcc tctttcgcat ggatggaagg ggcgattgaa gcaatagcag aagaaccaca
      721 aacagcgttt cctccagcca tcatcaaaga agaagtatcg ctgaattgca gttttttacc
      781 aatcaagtaa gcaccgatga tagtgactga catttgcagt aacacaaaaa caattccttt
      841 tacaccaatt tgagcaatcg tttgagctgt gacagttgca cctagtaaaa aaacagagaa
      901 ttctaatagt ttgctttcag caaattttgt gccgattgct agaacaggct gtttaaaaaa
      961 agtatttcct aataaaatac ccaataaaat agcaatagtc gctccaccta actgtggcag
     1021 ataaatagca agaagcctgc tgaaggcagc gatggcaaaa gaaagcagta aaccgggtaa
     1081 gatttcgcgg accgctgaat cctttacaga ttttattgga tgtgacaatg atttcatttg
     1141 atcggttcct ttcgtcaata aatactaaaa gcatactcga acatagtagc actaaaccag
     1201 tattttgaca aaagaacaag aggtcgtgac aaaagtcttg tacgacctct tgtttcgaat
     1261 aaacggtgtt ccagaagtag cttctacgga aataagccga aatttcacaa aaatttgaga
     1321 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctttt acgacctctt
     1381 ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccagta caaaaacatg
     1441 aacagctgtt tttgtttggc cgttcttatt acttgatgca gaacagcttt ttcacaacct
     1501 attgaatcga ttattaagcg atttttttag aaacaaattt tccatgtttg ctatgttctt
     1561 taattaagaa acgtacgtaa tgagcaagaa cgtagaagac aactacactt gcaaacagac
     1621 attccgcaac ttttaagaaa tccaatacgc tactatagat acctaaactg ttgaagtaat
     1681 gaacagtagt aaatatagct gttgcagaga taacaagcgg gaatgtaaag gctgcatagc
     1741 ttggataaaa acgaagcttg aacaaacgtg gaagcataga cagcacaacg aaatacaata
     1801 cttgagataa agcgaacaat gctacgacaa gataaaaatt aggttcagca aagtttttaa
     1861 gataacctgt taggcaaaga gaaccaggtg ctgcaacgat cgtaatcagc ggacgtgtcg
     1921 gatgagccat atttcttacg aaaaagacac gtttgatgat gatcggcaat aagaacgcat
     1981 agcagatcaa tgaaatccaa aagaaaattt ttccaataag tgggaagaag ttcccggcag
     2041 ttacagtgat gaccccaaat ccaacgtaaa tgataaacca gcttggataa attgcttcaa
     2101 ttgaaagatc agctttgata acatggtgat aattgaaata aaagacaagg atcacttgga
     2161 agataacagc gcacagccaa atgaatttga caattggtgc aatactttca tatgaaacaa
     2221 aataagtaca aataaccatg agtgacatcg taaaagttgg tgaaacggag gcgatgattg
     2281 gatcatgcaa cgtttgtttc gtgtgttcga ataagataaa caatttaaca aaaatgaata
     2341 tcataagaat catagagata ccgccagtta gattgcctaa agcaacatgg tgatagtctt
     2401 taaacagatt tccaagggaa gctaacccaa ggatcaaccc gcagatcggg atcggaataa
     2461 ttttcaaaaa gttcttcata tataatattc tcctttttat ttaatagatt ttagtaccaa
     2521 gatgaacgaa tacattgtaa aatgaatagg aatgtatgat tacgagcaag cagacagctg
     2581 attgtcttca tctaagttac tagttggatc agcaactgtt ttgttgtaca tttctattat
     2641 agatggtttt ttgtatattt aaatcaaata gtttttatag taattatcat aaaaagttat
     2701 ggaagtggtg ccatgtttaa gttattaacg acttttcgag cagtatatga aacaagaaat
     2761 ttttccagag cagcagaagt actgttttta tctcaaccag cagtctcgaa tcaaatcaaa
     2821 cagttggagc gggaattgaa tatcgatctt tttcaacgta acggaagaaa agagatgctt
     2881 ccaactaagc aagccgactt attatatgaa cggacactgg ttttgttaga agaatgggaa
     2941 gaaactcagc aaaaattgct caatgaacaa aatgaagaag aagtatgtcg gatcgttgct
     3001 tcccacacct ttgctgtcta tcttttgcct cagttgatga aacatctgat caaaaggttt
     3061 cctaaagtgc attttgagat cgaaatcacg aattctcatg aatcattaga acaagtagcg
     3121 aaacatgaat ccgattttgg ttttatcgaa aagccattag agacttccgg cgtacaaaga
     3181 tactctttga tggaagacca gctagtcatc gctggggacc caaaaagcaa attgtggctt
     3241 gtaagagaag caaactctgg tgtttttcat tacacgcagc gttattttga agagatgaat
     3301 atccaagata agaagatgat cgtgaaaagt aacgatgtaa ttgttgcgtt gctgaaggaa
     3361 ggaatcggtc gctcgatttt gtctaagcgc tctgttccag aaaatgtgcc aatgactccg
     3421 ttgaatttcc atcgatattt ttatttcatc caacgaacca atctggtttc tgatcagctg
     3481 ctagagattg ccgaagagat ccttcgatac aatgaaagca cagaaacttc tcacagcgag
     3541 gagaatctca gctggtagat aattgcatgt atcaaaaaaa gttctatgat acactaaaga
     3601 taagcagtag gcttttgtac tgcatttgct caagcaactg cacaaacgga aagttaattt
     3661 ttcccaaatg tctatggaaa gactttccat tatctaactg ggccgctata ataatcagca
     3721 gtatggtttt taagaaaagg gatgaaacat ggaaaaaaaa ttaaaagctt cgaacgataa
     3781 tcgtattgat tatggcgtga ttttaccagt ctttctgtta tgtttgattg gtctgctctc
     3841 attatatgta gcacttagcc atgatccgaa caatccagca gtcttgaaag gcgtagggat
     3901 gcaagttgtc tggtatttag ttggagcggc agcaatcgtt gtcatcatgc atttgaattc
     3961 caaatggatc tggaaactga ctccttacct ttatggtttg gggttagtgg taatgggact
     4021 cttgctcaaa ttctatgatc agaatttagc tgcttcaaca gggtctaaaa actggtttcg
     4081 attcggtgcg tttactttcc agccagcaga gttgatgaag attgcgtaca tcttgatgat
     4141 ggcactgatc gtaactaaac ataatacgca agtaaaagaa cggacgatga aatcggactt
     4201 ttggctgatt gggaagctct tgatcgtgac aataccggtt ttagcattga tcatggccca
     4261 agatgacttt ggaacgatgc ttgtctttct tgcgatcttt ggcgggattt tcctgatgtc
     4321 tggaatctct tggcgcatca tcgcacctgt agtaatcttg gcagtagtcg ttggagcagg
     4381 aacgatcttc ttggttacga ctgaaggcgg aagagacttg ctttataaag ttggattcaa
     4441 atcttatcag tttgcacgta ttgattcatg gctggaccct ttccatgata cgtcaggaat
     4501 gagttatcag ccggcccaag gacttttggc aattggtacc ggtggcttgt ttggtaaagg
     4561 attcaacgta agcaatatct atgtgcctgt tcgtgagtcc gatatgattt ttaccgttat
     4621 cggtgaaaac ttcggcttta tcggtggtgc attcgtcatt ttcttgtatt ttattttaat
     4681 ctatcgtatg attcgggtgt gtttcgatac gaataatgaa ttttacgcct atatcgcaag
     4741 cggtttgatc atgatgctgc tgttccatgt ctttgagaat attggagcaa atatcggatt
     4801 acttccttta acagggatcc cattgccttt cattagtcag ggaggatcct cgattttagg
     4861 gaacatgatc ggtatcgggt tgatattatc gatgagatac caaaatgaag cacctgcaag
     4921 atcttcaagg aggtaagaaa attgtacacc ttttactggt acccgaaatg ttcaacatgc
     4981 aaaaaagcca aagcgtggtt agaccagcat ggcgttagtt atcaaaccgt tgatatgatc
     5041 aaagaagtac cgaaggcctc aacgattgag acttggctag aaacgagtca agtaccaatc
     5101 cgtcgcctgt tcaacacaag cgggatgaaa tatcgggaac ttggactgaa agatcaagta
     5161 ggcgagttta cggcttcaaa agcgagccaa gtcttatcga cagatggtat gctgatcaag
     5221 cgaccactga tcttacgaga ggaccagctt gttgcaattg gattcaatga aaaaatttat
     5281 gaaggagaac tgatataaaa tggcaggcaa gcatttgaag aaaaaggaca acctttggat
     5341 cttatttaat ggaaaagaat actgtgttgg tttgacaaat gaagcgcaag aagaacttgg
     5401 agacatcacg tttgccaatc tgccaaaagc aggacaatcg ctaaaaaaag atgaacctct
     5461 gatcgaagtt gaagcggaaa aagcggtcaa tgaatttgcc agcccgctaa cgggtacagt
     5521 ttcttctgtt aatgataaaa tcggtgaaga tatcaatatc ttgaatgatt cagatgaaat
     5581 gaatgcgtgg atcttaagtt tcaaagatgt tgatccaaaa gaatttgacg aattataaaa
     5641 tcagaaaatc acttgaaaaa agttgacact gattcttcca gttgctataa tcataagcaa
     5701 tcctatatga aacgagaaag ctttgaaaag aagagtacat gtcacggacg tttgaagaga
     5761 gccctgtttg ctgaaaatgg gtaacgaaaa tgagatggaa gatggtctta gagcagagta
     5821 agcgagcgta agcaaactta ctacgggagt gcccgttaca gcgcgacagt atacggaaag
     5881 aagctaagtc caagtactgg aagaggctgt tattgcgaag taacggtaaa tcaaggtggt
     5941 aacacggaag ttcaagcttt cgtccttttg cttaaggcaa gagggcgaag gcttttttgt
     6001 ttgaaaaatt ataagagaaa gatgtgaatg gcatgtcatt aatccaacta acgaatgtca
     6061 aaaagacatt caatgggaaa aacggaaaca tccaagccgt caatgacgtt tcactaaacg
     6121 ttgaaaaagg ggatatttat ggaatcgtcg gctattctgg cgcaggaaaa agtacattag
     6181 tccgtctcct gaatggactt gagcttccca cgagcggcga agtgatcgta aacgatcaag
     6241 atattacaaa attaaaaaac aaagaattac gtacattccg aaagaaaatc ggtatgatct
     6301 tccagcattt caacctgtta tggtcaagaa ctgtacttga aaatatccag ctgccgctgg
     6361 aattagctgg cgtaccaaag agcaaacgta aagaacgggc agaagaactg ttgcgtttag
     6421 tcgggttaga aggacgcgga caagcatatc cttcacaact ttccggcgga cagaaacaac
     6481 gcgtcggtat cgcacgagca ttggcaaatg atcctgagat cctgctttgt gatgaggcta
     6541 ctagcgcatt ggacccgcaa acaacagaag aagtattaga cttattgtta gcgatcaata
     6601 agaaattgaa tttgaccatc gtcttgatca cacatgaaat gaatgtgatc cgcaaaatct
     6661 gcaacaaagt cgctgtgatg gagttgggga aagtcgtaga agaaggagac gtattgactg
     6721 tcttcagaca accgaaaaaa gaagtaacca aacgctttgt acaacaagac atcgaaccag
     6781 aagctgattc gacagaacta ttagctgaac tcatcaaaga aaatccaagc ggccgtttgg
     6841 caactttgac ctttagcgaa agaaatgcca acgagccagt catttcccaa gcgattcgac
     6901 gctataacat cgatgtcaac gttgtgtatg gaaagatcaa acaagtgaaa gaaggttcct
     6961 ttggttcatt gactgtcttg atgactggtg aaaccaaaga attagatcaa gcagaagtgt
     7021 tcatcaaaga acaaggagta ggaattgagg tgatccatcg tggataaaag cttttcagaa
     7081 acatatctag attttaaaca aatcaacccg gaaacgatga atcaggctat ccttgataca
     7141 ctatatatga cattaatctc aatggtctta gtgacgatca tcggattagt tttgggattg
     7201 atcctttact ctattggacg caaaaacaaa ccgtatgctc gaatcattta tggaatcgta
     7261 tcgatcatca gtaatatttt ccggtcaacg ccatttatga tcctgatggt tttgatcatt
     7321 ccatttacta aagcaatcgt aggcactatg ctaggtgcgc aagcagccat tccagctctt
     7381 gttttatccg cagcaccatt ttatgcccgt ctagtagaga ttgcttttcg tgaagtcagc
     7441 cctggtgtac tggaagcagc agatgcaatg ggagcaagtt actgggaaat catctggaag
     7501 gtcttgatac ctgaaagtat tcccgcattg atttctggat taacagttac aacgatctcg
     7561 atgatcggat ttactgcgat ggccggcgcg atcggcgcag gtggacttgg aggtcttgct
     7621 tggcaggaag gctaccagcg tggtaatcta acagtgacat tcgttgcgac tttaattatt
     7681 ttactaatcg tatttatcgt tcaaggaatc ggtgatttct taactaagaa aacagacaaa
     7741 agataatagg atataggggg atgtaggata tgaaaaagaa aatttttgga tttgcagcag
     7801 cgcttttgct gactgtagga ttagcagcat gcggtaacaa cagcgacagc aaagattcag
     7861 caaataaagc agacgacacg acattgaaag tcggcgcttc accgacaccg cacgcagaga
     7921 ttttggaaca tgtgaaacca ttattgaaag atgaaggaat cgatcttgaa atcgtcaaat
     7981 tcgatgacta tgtattaccg aaccaatctt tggaagaagg cgacattgat gccaattact
     8041 tccagcatat tccttatttg aacaaagaaa tcaaagaaaa aggatatgac tttgtcaatg
     8101 ccggtgctgt ccacatcgaa ccaatgggct tgtactctaa aaaagtcaaa gatgtttccg
     8161 aattaaaaga tggtgcaaca atcattactt caaattctga atcagactgg ggacgcatca
     8221 tcacaattct acaagatgct ggcttagtca aagtcaaaga tggagtagat ctggaaacag
     8281 caacattcga tgatattgaa gaaaaaccta agaacttgaa gttcaaccat acgatcgacc
     8341 catcactttt agcttctacg taccaaaatg atgaaggtga cctagttgca atcaacgcaa
     8401 acttcgctta cggcgcaggg ttgaatccat tagaagatgc cgtattgctt gaaaaggaca
     8461 actctcctta cgtgaatatt attgcgactc gtaaaggtga tgaagacagt gacaaaatca
     8521 aaaaactgat tgaagtgctt caccgcaaag aagtccaaga atggatcaca gacaaatggg
     8581 gcggctctgt taaaccagta aaggctgatg caaaataagg tatggaacaa aaaagatgag
     8641 aaataagtct gtaatggctt atttctcatc ttttttatac ctattctaat ttagaaatca
     8701 aaattgttta ataataaata aaagatattt gattttagta aggaaggtca ttgcgcttca
     8761 ttctcaatta gtctaaaatg agtgagaacg aaagagaatt tgggggtata atgcatgtct
     8821 gtcttagaaa taaaaaatct acatgtgtcg atcgaggaaa aagaaatcct taaaggcgta
     8881 aacctaacaa tgaaaaccgg agaaatccat gccatcatgg gacctaacgg cacaggaaaa
     8941 tctaccttat ctgccgctat catgggcaat ccaaactatg aagtaacaga aggcgaaatt
     9001 ttgtttgacg ggaaaaatgt tttagacttg gaagtagatg aacgtgcgcg acttggctta
     9061 tttttagcta tgcaatatcc aagtgagata ccaggtatca cgaatgcgga attcatgcgt
     9121 gcagcgatca atgcgaaacg tgatgaaaac gacaaaattt ctgttatggc atttttgaaa
     9181 aagttagatg aaaaaatggc tttactaaat atgccagaag aaatggctga acgttatctg
     9241 aacgaaggtt tttctggcgg agaaaagaaa cggaatgaga tcctgcaatt gttgatgtta
     9301 gaacctacat ttgcaatttt agacgaaatc gattcaggtc tggatatcga tgctttgaaa
     9361 gtcgttgcta aaggagtaaa tgaaatgcgc ggagacaatt tcggtgcatt gatcatcacc
     9421 cactatcaac gattattgaa ctatatcaca cctgatgtcg ttcacatcat gatggaaggc
     9481 cgagtagtca tgacagggaa tgctgaccta gctaaacgtc tggaagcaga aggctacgct
     9541 ggtatcagta aagaattagg aatcgactat aaagaagaag aagcgtaagg gggagcaaga
     9601 atgaaagaaa aaaactggct cgattatctt gacgcggtaa atgatttttc tttaagtaaa
     9661 ggcgaacctg attggatgag aaccttccgt caagacgcac tagcgaaagc agatgaactg
     9721 ccattgccgc atatcgatcg ggtgaaattc catcgctggt cgttatttga tgtgaaagaa
     9781 acacaaacca tcagtgaaac aggcacgatt ccggcatttg atgcaatgaa agacaatcct
     9841 gtgcttgtcc aacaaggttc atggacgatt tttgaacaac tgcctgtgga attagcagaa
     9901 aaaggagtca tttttaccga cttgttcact gcaatgatcg aatatccaga actagtccaa
     9961 gaatattata tgaaaaaagc agtaaacatg aacgaagacc aattgactgc actccatgta
    10021 gcttttatga acagcggtat tttcctgtat gtgcctaaaa atgtcgtgat cgatgaacca
    10081 ctcgagtcct tattcattca agatggcgca tccgatgagc acttcttcaa gcatgtgttg
    10141 atcgtggcag atgaacatag tgaattttct tatctggaac gtttccagac aaccaaagaa
    10201 caagtagcaa aatcatcagg taatatcatc gtggaagtca tcgctaaagc tggttcaaaa
    10261 atcaaatatt cagccgtaga tcaattagga gaaaatatta cttcttatat gaatcgccgt
    10321 gggcacattt tacgagatgc ttctgtcgac tgggcaattg gtgtcatgaa tgacggtcat
    10381 gtgatcgctg actttgactc agatttagca ggagaaggtg cacacgcaga agtcaaaatc
    10441 gtggcaatca gctcgggcag acaaatccaa ggaatcgata cacgagtaac caataaagcc
    10501 cctcatacga tcgggcatat tttgcaacac ggagtgatcc gagaaaaagg aacattgacc
    10561 tttaatggta tcggccacat cttgaaaggg gcaaaaggtg cagatgccca acaagaaagt
    10621 cgtgtcttga tgctttctga taaagcacgt ggtgatgcga atcctattct tttgatcgat
    10681 gaaaacgaag taactgctgg acatgctgcc agtgtaggac gagttgatcc agaagaaatg
    10741 tactatctga tgagtagagg attgcataaa gaagaagcag agcgcttagt gattcgagga
    10801 ttcctcggtt ctgtcttaac agcaatccct gtagaacaag ttcggaaaga attagtcgaa
    10861 gtaatcgaag ggaagttgaa tggatgatcg atgctaaaaa gctacgcaat gattttccta
    10921 tcttatttca agacgtcaat gatgaacctt tagtttatct ggataatgca gcgacgaccc
    10981 aaaagccaaa acaagtactc caggcattag cgtactatta cgaacacgat aacgccaatg
    11041 ttcatcgcgg tgtccatacg ttagcagaac gcgcgaccca tgattatgaa caagcaagag
    11101 aaaaagtacg ggcgttcatc catgcaaaag aaacagcaga agtgctgttt actcgtggta
    11161 cgacgacaag cttgaattgg gtagccagaa ccttcggcga ccgctatatc aaagcaggag
    11221 acgagatagt gctctcttac atggaacatc attctaatgt tatcccgtgg cagcagttgg
    11281 caaaacgaac aggtgctgtc ttgaaatata tcgaactaac agaagaaggc ttcctggata
    11341 tggaggatgc agcgaaaaag attacggaca aaaccaaaat cgtctccatt gctcatgtat
    11401 ccaatgtctt aggtgtcttc aatcctgttc aagaattagc tgaacttgtc catgaaaacg
    11461 gcggggtcat cgtagtagat ggtgctcaat ctgccccgca catggccatt gatgtccaag
    11521 cactagactg tgatttcttt gctttcagcg gacacaaaat gtgcggacca acaggtattg
    11581 gtgttttata tggtaaacga aaatggttag aagaaatgga acctgttgaa tttggtggcg
    11641 aaatgatcga ttttgtcgaa ttatacgaca gtacatggaa agaactacct tggaaattcg
    11701 aagcagggac accaaatatc gccggcgcta tcgcattagg ccatgcaatc gattatttgc
    11761 aaaaactcgg tatggacaat atccatcgat atgaagaaga actagccgct tatgtgttgc
    11821 caaaattaca agcaattgat ggattgactg tttatggtcc acaagacccg aattttcgga
    11881 caggagtcgt tgcattcaat ttggatgggt tacatcccca tgatgtcgcg acagctttag
    11941 acatggaagg ggtagccgtc agagcaggtc atcattgtgc gcaaccgcta ttgaagtacc
    12001 ttgaagtatc agcgactgct cgcgcgagct tttatttcta caacacaacg caagacgccg
    12061 accgtttagt tgaagcaatc atggcaacaa aggagttttt ccaacatggg actatctaaa
    12121 ttagacaatt tataccgtca agtgatcttg gatcattctt cccatccgca tcatcgtgga
    12181 agcttgactg actcgacaag caagatcgaa ttgaacaacc ccacatgtgg agatgtgatc
    12241 gaattgcaag tcgatgtaaa agacggaatc atcaaaaata tcgcctttga cggcagtggc
    12301 tgctccatca gtacggcaag tgcctcgatg atgacagacg ccgtcatcgg aaaatcaata
    12361 gaagaagcag aacatttaac tgtagacttt ctacagcttg tccaaggaaa agaagtcaaa
    12421 gatcccgacc aattaggcga tgctgctatg cttggtggtg tagctaaatt tcccgctcgg
    12481 atcaaatgtg cgacacttgc ctggaaagca ctaggaaaag caatggaaaa cgatggtacc
    12541 gcagctttcc atcattacca caaggaagag gagtgagaac atgggcgtac cagaattaga
    12601 agaatataaa ttcgggtttc acgatgatgt cgaaccggtt ttcagtacag gagaaggctt
    12661 gacagaagaa gtgatccgtg agatgtcaag aatcaaaggc gaaccagaat ggatgcttga
    12721 attccgtctg aaatctctag aaacatttaa caaaatgcca atgcaaacat ggggccccga
    12781 cctttcagac atcgatttcg atgccatcaa atattaccaa aaaccaagtg acaaacccgc
    12841 tcgtgactgg gaagacgtac cagaaaaaat caaagataca tttgaaaaga ttggtatacc
    12901 agaagctgaa cgagcttatc tagccggtgc ttccgcacaa tatgaatcag aagtcgtgta
    12961 ccataatatg aaagatgaat tcgaaaagtt aggaatcgtc tttactgaca ctgactctgc
    13021 tttgaaagaa taccctgaat tattcaaaga gtacttttca aaacttgtac cgccaaccga
    13081 caacaaatta gcagcgctga attcagccgt atggtcaggt gggaccttta tttacgtgcc
    13141 aaaaggcgtc aaagtcgatg ttccattaca aacgtacttc cggatcaatg cggaaaacac
    13201 ggggcaattc gaacgtacgc tgatcattgt tgacgaagga gcaagcatcc actatgtcga
    13261 aggttgtacg gcacctactt attcaagtaa cagcctgcat gccgccatcg tagaaatctt
    13321 tacaagaaaa gatgcgtatt gccgttacac aacgatccaa aactggtcag acaatgtgta
    13381 caacttagta accaaacgag caaaagcata cgaaggcgca accgttgaat ggatcgacgg
    13441 aaacctaggt gcaaaaacaa cgatgaaata cccaagtgtt tacttagatg gaaaaggtgc
    13501 acgaggcacc atgctctcca tcgcctttgc aggagcaaac caaatccaag atacgggtgc
    13561 aaaaatgatc cacaatgcac caaatacatc tagctctatc gtctctaaat caatttcaaa
    13621 agacggcgga gaagtaaact atcgaggaca agtaaccttt gccaaaaaca gtgcgggttc
    13681 gatctctcat atcgaatgtg acacgatcat catggacgaa tggtcgaaat ccgatacgat
    13741 cccattcaat gagatccaca acagccaagt ctctttagaa cacgaagcca aagtctcaaa
    13801 aatctcagaa gaacaactct actatctgat gagccgaggc ctgaccgaat ctgaagcaac
    13861 tgaaatgatc gtcatgggct ttgttgaacc attcaccaaa gaacttccaa tggaatatgc
    13921 agtcgaactc aatcgattga tcagttatga gatggaaggg tctgtaggtt aataactatt
    13981 tcattgctcg aaacgttgat attaaagcgt ttcgagcgtt tttttatttg tattttaatg
    14041 gttggctacg tttttggcta cgtttggaat tattcgccca taaattttgc aaatcggtca
    14101 ccagttttct taacagattg tggtgtgaca tgagcataga tattcatggt agttttaata
    14161 tctttatgac ctaatcgttc ctgtacttct tttatcgacg caccactttc aaacaataag
    14221 ctacagtggg tatgcctaaa tccatgtgga gtaatttgag gctccatcgg tgtctttttg
    14281 taaatccagt ctaaccaatc gtttactact tgggggtagt acaacttgtt tgtgtttgta
    14341 gtgaaaagaa actgttttgg tgaattcgtg ttatagccat ataagatcat tattttcatt
    14401 tgttcttgtt gccaaatacg cagttgttta agtgtaaaat catcaagctg aatcgttctt
    14461 gatgaatttt tagttttagg tacttgtaga actacttgat taaactcgtc ttgagcgata
    14521 gttttaccaa ttgttattgt tttattgaaa tagtctatat cttcccattg aagcgccaat
    14581 gcttcgcttt tacgcattcc tgtataagct aataaacgga aaaaagtgta cagtttaact
    14641 gaattatttt ctttagtatt ctgtaaaaac agttgtaatt gatctttcgt gtaaaacttc
    14701 aagggttgtt cttcttcaat ttttcttgga agcaatgttt tagacatagg attatcctta
    14761 cagacttcca ttgctactcc aaatttaaat atttgagcag ttgtttttct caagaaacca
    14821 tattgtttta atgttttgtg ccattcattc acctgttttt ggcaatagct aacacttatt
    14881 ttatccaatc gtaattcccc aaaagcaggt aatatatggt ttttgcaaaa acgttgagca
    14941 acggcaacag aagacggttt tactcttgtt ctgtagttat caatccataa ttcatacaac
    15001 tcttgaaaag ttgtaacatt gattttaaac tcttgttttt ctttaaagtc tacaagtagt
    15061 ctattaagtg ctgtttgcgc ctcagctttt gatttgaatc ttctacgctt tgtacgtata
    15121 tcttttccag tcttgggatc tttccctaaa taagtttcaa atccccaata tttttcgcca
    15181 ttttgtaatt tataactaaa aaatgttgcc atttgattct ctccttactt cattattgca
    15241 ggtagagaag t
//
LOCUS       DAZAID010000068        15225 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000068 DAZAID010000000
VERSION     DAZAID010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.068"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..315
                     /locus_tag="ACG9BE_002007"
     CDS             <1..315
                     /locus_tag="ACG9BE_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN3032744.1"
                     /translation="DEINQKIAEINQLNTDWNNRLQTEQNKFNDAQNTVNSLNQQINQ
                     LSNEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTANRSQEIVTEHT
                     GK"
     gene            complement(597..669)
                     /locus_tag="ACG9BE_002008"
     tRNA            complement(597..669)
                     /locus_tag="ACG9BE_002008"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(634..636),aa:Lys,seq:ctt)
     gene            1050..1619
                     /locus_tag="ACG9BE_002009"
     CDS             1050..1619
                     /locus_tag="ACG9BE_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFN3032745.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1606..2466
                     /locus_tag="ACG9BE_002010"
     CDS             1606..2466
                     /locus_tag="ACG9BE_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN3032746.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2656..3360
                     /gene="yycF"
                     /locus_tag="ACG9BE_002011"
     CDS             2656..3360
                     /gene="yycF"
                     /locus_tag="ACG9BE_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFN3032747.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3365..5200
                     /gene="walK"
                     /locus_tag="ACG9BE_002012"
     CDS             3365..5200
                     /gene="walK"
                     /locus_tag="ACG9BE_002012"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFN3032748.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5197..6513
                     /gene="yycH"
                     /locus_tag="ACG9BE_002013"
     CDS             5197..6513
                     /gene="yycH"
                     /locus_tag="ACG9BE_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFN3032749.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6514..7383
                     /locus_tag="ACG9BE_002014"
     CDS             6514..7383
                     /locus_tag="ACG9BE_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFN3032750.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7438..8247
                     /locus_tag="ACG9BE_002015"
     CDS             7438..8247
                     /locus_tag="ACG9BE_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN3032751.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8350..8979)
                     /locus_tag="ACG9BE_002016"
     CDS             complement(8350..8979)
                     /locus_tag="ACG9BE_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN3032752.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9149..10441
                     /locus_tag="ACG9BE_002017"
     CDS             9149..10441
                     /locus_tag="ACG9BE_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3032753.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10517..11179)
                     /locus_tag="ACG9BE_002018"
     CDS             complement(10517..11179)
                     /locus_tag="ACG9BE_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN3032754.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11404..11688
                     /gene="groES"
                     /locus_tag="ACG9BE_002019"
     CDS             11404..11688
                     /gene="groES"
                     /locus_tag="ACG9BE_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFN3032755.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11739..13364
                     /gene="groL"
                     /locus_tag="ACG9BE_002020"
     CDS             11739..13364
                     /gene="groL"
                     /locus_tag="ACG9BE_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFN3032756.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            13777..14964
                     /locus_tag="ACG9BE_002021"
     CDS             13777..14964
                     /locus_tag="ACG9BE_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFN3032757.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         5120 a         2598 c         3231 g         4276 t
ORIGIN      
        1 gatgaaataa atcaaaaaat agctgaaata aatcaattaa acactgattg gaataatcgt
       61 ttacaaacag aacaaaataa atttaatgat gctcaaaata ctgttaatag tttaaatcaa
      121 caaattaatc aattgtcaaa tgaaaaaaat actattaaaa accaattgca aagtactgaa
      181 caaaaactag aaaaagcgca atcagaaaac agtaatttga ataattatat tgaaaaactg
      241 gaaaaagcca aaagcgatgt agaagacaca gcaaacagat cacaagaaat cgtgactgaa
      301 catacaggaa aatagatgca ttttttagtt taaatttctt taacaaaata gtattttgtt
      361 gctaaataat ttttatttga atattctaca atgatatctc ccctcaaaat ggaggggaag
      421 tacatactgt gataatctac aggtttattg actgattagg atgttattaa aaaataaaat
      481 actaaaatgc atacccgaat ttcatacccg aaaaataaaa aatgccacca cgtacactct
      541 tttcgaaact acctaagtag ccaagaatgt actggtgacg tgggtttggc acccaatggg
      601 ccgtgagggg ctcgaacccg cgacccgctg attaagagtc agctgctcta ccaactgagc
      661 taacggcccg tacaaaaagt acttaataat agtagcacta agaattttaa aatgcaagac
      721 tacatttaaa gaaaatcaga aaaatcttta cttccttata ggaaatcatt catagattat
      781 tcacatttgc tcgctattat ataagcatac caacaaacaa agttcttgca agagaacaaa
      841 ccctaacact tttttcattt tttactcctt tcccgccgtc tccccggcgg gtctcctgtt
      901 cttaacgata aaagacaaga aggcttagcc ttcttgtttt ttgttttcat ggaatcccgc
      961 taatcaaatc acctagttaa agcaactaga ttgaatgttt atcataagtc tgatatcatg
     1021 tttatgaaat gaaaaaagga gcggttatga tggaaaaaat gcgtcagcaa ttaatggagt
     1081 gggggaagga actagaaaca ttccgtctgc ctcactggga agaactccct gacttagaac
     1141 tttatatgga ccaagtcaga acgttagttg atcgttatct ttcacctgtg atacaaggag
     1201 aaaagcatcc attgcttacc tcttctatgg taaacaatta cgttaaacta ggtttgatac
     1261 cagcaccagt taaaaaaaga tataacaaag aacacgtagc atttttactt gcaatcacta
     1321 cgttgaaaca agtattgact attcctgaaa tcaaagaagg gatattgttc caaggaaaaa
     1381 cagtaggaat tcgtgaagcc tataatctat tttgcgatga acaagaagca gctgtgtgga
     1441 tggtcagcca attagcacaa ggtaaatctc atccgcaaaa attcgaccag gcaacccctg
     1501 tagaatatat tgctgtaaga gcggcaactt tatcgtttgc aatgaaactt cttgcggaaa
     1561 aaacgattgt tctagaaacc gaatatttaa aagaggagaa aacaaatgaa aaacaataaa
     1621 atcgcaattt tagtagattc aggtacggat gtgccagaag aattgataaa aaaatacgag
     1681 atgtatatga tcccattgaa aattatttat aaagatcagg tatatacaga taaaattgac
     1741 attacctctg aagagatcta tcaacgtttg ccagaggaaa tccctggtac atctttgccg
     1801 gatggtgagt cgatcacaga aatctttgag caaatcaaaa aagacggtta tgaaaaggta
     1861 cttgcggtaa caatttccag tggattgagc gggacatata atgtggttcg tttaattgca
     1921 caacagcaag aagaattgga agcatatgta ctagatacga aaaatattgg tattggtgca
     1981 ggtttttctg cgatccaagc agcaaaatgg ctggaagaag gagtagaatg ggatcaactg
     2041 atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt tctttaatgt tgcgacattg
     2101 gaatatcttc aaaaaggtgg acggatcggt ttagttgctt ctattgtagg aaccgcttta
     2161 aagcttaacc caatcatctc atgtaatgaa gaagggattt attacacagt aggaaaagct
     2221 aggggacgta aaaaaagttt ggataaaacg gttgcttacg tcaaagaaaa aatcggtaat
     2281 gcaaaggtct tcaacttagc tgttgcccat ggggatgcga aagaagaagc aattgaaatg
     2341 gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa tctactttgg ccagatttct
     2401 ccggcattag tagtccacac tggtccggga ttattgggtg tcggtgtaca agttttagaa
     2461 ggataaatca aaaaaaggaa acactgaggt gaaaatcagt gttttccttt tttattgaag
     2521 ttagaacaaa aaataataaa gtattttgca ctttcatttt aattttctct tttgttttat
     2581 aggcaaagat gaaacaaaag cagaaatacg gtataatgag aaggtatgac aataatatat
     2641 aagaggtggc tctttatgaa aaaagtttta gttgtcgatg atgagaagcc gatttcggat
     2701 atcgtcaaat tcaatttagc caaagaaggc tatgatgttt atactgcata tgatggggaa
     2761 gaagccttgg aaaaagttgc tgaggtagaa cctgatttga ttttacttga tttgatgcta
     2821 cctaaaatgg acggcttaga agttgctcgt gaggtacgca aaacttatga tatgccgatc
     2881 attatggtaa cagccaaaga ttctgaaatt gataaagtcc taggcttaga gttaggcgcc
     2941 gatgattacg tgaccaagcc attttccaac cgagaattag ttgcacgtgt caaagcaaat
     3001 cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg cagcacctgc ggaattgacg
     3061 attggtgatt tgacgatcca tccagaagct tacatggtga cgaaacgagg agaaacaatc
     3121 gagttaacac atcgtgaatt cgaactactc ttttacttgg cgaagcacct tggacaagtg
     3181 atgacaagag aacatttatt acaaacggtt tggggttatg attattttgg agatgtacga
     3241 acagtggatg taactgtacg tcgtttgcgt gaaaagatcg aagataatcc aagccatcca
     3301 aattatttag tgacacgtcg tggtgtcggt tactatttgc gtaacccaga acaggagtaa
     3361 ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat tttaagatcg ctattacctt
     3421 tattctgatt ttattgatct cgatcgaaat tattggtgcc tactttatac gtggtttaga
     3481 acgttccacg atcaatacat ttattaaaga tatgaatcaa acggtggaat cattagcaac
     3541 aacaatcagc ccggaattga atcgaaaaga taatgcagat gatgaagagg taaatgcgaa
     3601 tatcaaaaga tttattgaaa acagtgcgac tagcgatatc atcgaaatac gggtcgttga
     3661 cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa caaagcgctg tgggaaagaa
     3721 aaatgattat gttgatatca atgatttcac gactaaacgt tatgttgctt tagataacga
     3781 cgataaacgg gtgaatatca atgttcaacc gatcctgtcc ccgaccggcg atgccgttat
     3841 cggggctctt tacgtcaaaa gtaatatcga acaaaaatat tcggaaatca ataacacagc
     3901 agtcattttc tttactgcct cactaattgc ggcatttatt tcaatgatcg tatctgtttt
     3961 agttgctcgt tcgattacac agccgatcgg tgaaatgaga gaacaagcca ttcgaatcgc
     4021 tagaggagac tacagtcgga aagtcaaagt gcatggacaa gatgaacttg gacagctggc
     4081 tgataccttc aatcagttag ctgaacgtat tgaagaagct caagatacaa tggaagctga
     4141 acgcaatcgt ttggatagcg tgctatccca tatgacagac ggcgtcattg cgacagatcg
     4201 caggggtaaa gtcattacga tcaatgatat ggctgtttcc ttgctagata tcaaaaacga
     4261 ggaagcaatc ggacaatcga ttttgacact tttagatatc gaagaagaat atacgttgcg
     4321 gaaattatta gaagatccaa acgaaatggt acttgaccgt tcaaccagta cattggaaag
     4381 tgatcagatg atcttgcgag tagacttttc tatgatccgt cgcgaatcag gttttatcag
     4441 cggattagta gctgtcttgc atgatgtgac agaacaagaa aaaacagaac gggaacgtcg
     4501 tgaattcgtc tctaatgttt cccatgaact tcgtacaccg ttgacaagta tgcgtagtta
     4561 tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa gtagcaccaa attttttgaa
     4621 ggtaacgcta gatgaaacag accgaatgat tcgaatgatc aatgacctct taaatctatc
     4681 tcgcatggat acaggtaata cccagcttca attagaatat gtcaatttca acgagttagt
     4741 gaattttgtt cttgaccggt ttgatatgat ggtgggaaat caggaaaaaa attataagat
     4801 ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta gatacagata aaatcatcca
     4861 agtagtcgat aatatcatga ataacgcgat caagtattca ccagatgggg gaacgattac
     4921 ttgtcgctta ttagaaacgc ataataacgt ggttttaagt atcaccgatc aaggattggg
     4981 gattcctaaa aaagatctga accgagtgtt cgagcggttc tatcgagtag ataaagcacg
     5041 tgcaagagca caaggtggga caggacttgg attagctatt tctagagaag tgattaaggc
     5101 gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa gggtcgactt tttatatctc
     5161 gctgccatat gaaccttatg aggaggaatg gtgggaatga gaatctctga aaaagtcgtt
     5221 cgtataggac tcattttgat ggttgcgtta agcatatact tctcttatgc aatctggctg
     5281 agcccagcag gaaaaagttc aatcaacgta gatgagacaa acactcaaat gatcgagtct
     5341 caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc atgttacatg gcaccattcg
     5401 ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc gattgcaatt gatcatcgat
     5461 aaagcaagat ttggaaagct ttctgaagta gtttcaggag acaaatcaca gtttgaacag
     5521 gtgaaaaatc tgaactctgg ttttgagttg gcctataatg cgcctttttt gctaagagaa
     5581 tacaaggaag catttgattt agatttagat gtgtcgaact tggcaaaaga aagcgaaaat
     5641 atgtatttta ctagaataca attcgatcaa ggaaataaaa aaatccgatt tttgaattat
     5701 tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt ggacagatat caataaagaa
     5761 ttaacagcaa aagacacaaa atggacagaa atgacaacag aaccatcgac gatcgacacg
     5821 caatatgcta cgaaacattc gatcaaactg aaaaagtata gttatatcct gtcacagcaa
     5881 ccctatacac tttttagaaa tgcttttttc cagaaaccag atagcgtgaa aaataatgat
     5941 gaaagcaatg aattgatttt ttacggtggg aatgaaacaa tgtctatcca ttcggaaacg
     6001 caagtagttg attttcgtgg agagctgcca gagaaaaaag aaaatgctag tgtattttct
     6061 gaaagtttcc cctatattag caaattaggc agttctttag ggaacctcag atattttgac
     6121 cgcagtgaca atacgatcga ttatcggata ttcgtggaag gcttcccggt ttttgggaca
     6181 gacagcagag gacagattgg tgtggaagtt ggcggtgaaa cgaatggttc tgtgcaagtg
     6241 aatatccaaa ccagtttgaa tacgattcaa gtgccgatac cttcagacga agaagtagaa
     6301 cttgcaagta cgaatgaaat catccagcag ttaaaagcta agggagcaga tagcaacaag
     6361 attggttcag tgatcgttgg ctatacatgg caaaatgtca aagaaacgaa tcgtgtcgtt
     6421 gatctcttac ctgaatggta tgtgaaatat aaagataact ggtattcagc taacgacctt
     6481 ctggaaaaat tacctggatt ggaggcgaat taattggatt tcaaaaaaat cgaatggata
     6541 tttttcgtcg cttttttagg gttgaatgtg tttttattca gcagttatca tgacgcggta
     6601 taccaagaaa acaatgtcat tcgctctaat cagactgaat caatccaaca acgtctggct
     6661 agcgacgata tcacttacac ggggaagatt tcgtctgaga acaagcaagg ctattatctt
     6721 agcgcggagc agaccaatct tagcacttca ttagcgaatt atcgcaaggc tggtaaaagc
     6781 ggcttgctgg caagtggtgt gtttttagac ggtgatgttt tgacgaaatc tttatccagt
     6841 tcagatgctg aaaagaacgt gatcgatcca gataaaatta gtagttcctt ggaagatttt
     6901 ttatctaaca aagacaatat tttattcggt aatgattatg tgtacacgaa aaacttttct
     6961 catttagaag aagaaccact agaaatccaa gcaagccaag agtatgaagg catcccattc
     7021 aatgatgata cagcaaaaat cgttctttct ttagaaaaaa acgataatga ttatagcatc
     7081 acgaaataca tgcagacgca tttatctgat atcgagcagc tgcgcgaaaa gacggaactg
     7141 tatacggaag aggaagcaat caatacattg tacatcaata acaaaatacc aagaggatca
     7201 aaaattttat ggcgtcagct agcttattcc tgtattctga aagtccggga aaaaaatgtg
     7261 tatgttcctg tttggcatgt agcgatcgaa acaagcgata aagtggtcca aattgagagt
     7321 gtcaacgctt tcagcaatac gatcgtgaca aacaatactg taccaaaagt ggaagatcaa
     7381 taaaaaagcg agtataatga aaaaagttta caaagtaaga agggaagtag gagtcaaatg
     7441 gctgaagaaa aaccagcttt taaaatcagt atcctagcaa gtggaagttc gggtaattca
     7501 ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg caggacttag cggtaaaaaa
     7561 atcacttcct tgatggctca aattgatcgg acaccggacc aattagatgg aatacttgtg
     7621 acgcatgaac atcgagatca tatacatggc gtgggtgtct tggctcgtaa gtatcatttg
     7681 gatgtttttg ccaatcagca gacttgggaa gcgatggaac cactgattgg aaatgtacca
     7741 gtggaacaaa aacatctatt tgagatgggg aaagtcagaa cattcggcga tatcgatatt
     7801 gagagtttcg gcgtctccca tgacgcagcg gctccgcaat tttatcgttt ccataaagaa
     7861 aatcggtcat tcgtcatgtt gacggacacc ggatattgca gtgattatat gcgaggtgtc
     7921 attgaaaatg ccgatggtta tttgatggaa agtaatcatg atcttgagat gttacgcatg
     7981 ggaccttatc cgtggaattt aaaacagcgt attttaggag atagaggaca tctttccaac
     8041 gaagacggtg cacttgtcat gactgatgtg atcggtaatc ggaccaaacg aatctatctt
     8101 ggccatctta gcaaggaaaa caatatgaaa gaacttgctc atttaacaat ggaaaatgtt
     8161 ttgaaagaaa aagattttgg cgttggacat gattttgaaa tctatgatac agatccagat
     8221 gaagcaactg agctattttc gatttaaaca gttcaatcaa taaaaatata aaaagcagaa
     8281 gataaaatca atcagggttg gttttatctt ctatttttat ttttttagca tattaaaaaa
     8341 ataaccgtat tatcgactga ctacactgat gtgctgcttt tcatgtttct catccagtat
     8401 ctcatctact aatgcaatcg cgtaatctgc catgctgatc gtactgtcgc cagcagcgtt
     8461 tctttgtaaa taatcatcac ttaacaaata agtacctgta cgtttgccat gtggatcaaa
     8521 taaagaagct ggactgatat aagtccaatg tagattttta gattgtttca attttaacaa
     8581 tccttcgtac atattgaaag ctgttggata gaagggtgca tttttaggta cttggtcaat
     8641 catacgagtc gtcttttctg tatctaagaa taacgaagag gcacccccaa cgacaattaa
     8701 gcgtgtatcg gttccttcaa gcatctcaat caaatgggac aaagaagttt gatgcatttc
     8761 ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca tcaaaagatg caagatcagt
     8821 agttgtcaac tcaaataaat ccttttgtat aaacggaaca tctgatggca atttttctgg
     8881 atgacgcacg attgccgtca cgtctaaatc tctggacaat gcttcgtcta aaataaatga
     8941 acctgcatgg ccagtagctc caatgagtgc aattttcata aaaaacctcc ttttttattc
     9001 ctgtccattg taagcagttg ctgaatattc gtcaaagaat ccgatttttg aaaagctcag
     9061 tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt aaattaagaa aactgattga
     9121 taaaaataga agttgaagga gataagaaat gaaagagtgg ttagatcgtg cctttttacc
     9181 aatgataaca tctgttgtga atcttagacc cttaactgct ttgcgtaatg gaatgactta
     9241 cacgatccca ataattattg ttggctcgct gtttttctta tttgctcatt ttcctgtgga
     9301 ttacatcgct cagtggatag acaaaacagg attttctatg tattttacac aagtatacca
     9361 ggctacattt gatgtgatag ccttttggat cgtttttgcc attgcatacg cttatgcaaa
     9421 agaagaagac atcgatcgct taccagtcgg cataacagcc ttgtctgctt atctactgct
     9481 tattcagcca ctaaatactc agatagatac tgtctggaca ggtgctgaag ggatactagg
     9541 agcagttaca actgggctca tcatcggatg gggctatgca cgtttagtaa gacttgcgaa
     9601 agggtggaag caaagagaga agatacctga aaatgtttgg cagtctttta cttcattgat
     9661 tccaatcatt gttatagtca cttttacgct tatcggttca gcggtttttc aacagttgtt
     9721 cagaatgact gttgttcaag gagtatggac atatgtccag cttcccattc aatcgttcat
     9781 agacacgttg tttggtgtgc ttttattagg tttttttgtt ccgtttttat ggttatttgg
     9841 tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc gtcttgcaag cgaattcatt
     9901 agcgaatgca gaaatcttag cctcaggaca agagttgacg gtggcaaatg gcggacatat
     9961 cgttactcag caatttctcg atcagttcat gacggttacg ggtgctggat tgacgttagg
    10021 ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa tacagagaat taggaaatct
    10081 tgctctttta ccagcctttt tcaacatcaa tgaatcaatt attttttcca caccgatcgt
    10141 gatgaacccg atgatggctg ttccttttat tttcgcaccg gtcgtttcag gattgattac
    10201 ctattccgct ttatattttg gattcgttcc tttatttaca gcagtccaag tcccttggac
    10261 aacaccgccg atcctctcgg gtttcttagt aggcggagtg cctgcagcga ttttacaagg
    10321 tatcgtgttg tcaattagtt ttttcattta tttcccattt ttgaaaaaaa tagattctgc
    10381 aaactgcaaa aaggaaagac agacaaaaaa tgatggagca gcagaaaaga tgaacgacta
    10441 aaaaacaaaa atactcctgt cgctttagat tgggaaaatc accaatctag gatgacagga
    10501 gtatttatag tcatgtttat actagccctt tttggacagc taattgaaca agaacaacta
    10561 atgtgttcat tcctacgtgt gtgatcatcg atgtccagat ccgaccagtt gctttgtact
    10621 ctaggctgaa gaagaaacct aggaaaaagt agattagcag atgaccatca ttatgagcaa
    10681 acgcaaacag caaagagctg atcactgctc cgacccaaaa atttgaatat cgactgatga
    10741 tccccagtat actgcggcgg aatacgaact cttccatgat gggtccagca acagttgtcg
    10801 ccaaaataaa agcgggtgct tccatgatca tctggatgat attttgtgta ttctcggaag
    10861 gggttgcttt gccaaacaat acagattcta acatgacagc gatgctttgc agaatcaatg
    10921 cgacaaagat tccaaccaat ccataaataa caatctttgg agcagaagct ttttttaccg
    10981 ctgattcgat cgctaagggt tcacgttgct taaaataaaa aatagctagg acaacggcgc
    11041 ctagtatata catgactgca gttgctgtga tcaattcaga cgtagatttt acaagtccta
    11101 tcgaagctag gatcaagggt gagataaaaa ttagaccata gcaaaaaatg ctgaggaagc
    11161 tatatttttt taaagacatg taattcctcc ttttaaggga taacaagcat gcaaggcaag
    11221 cttatatcga ttgatttcaa ttatatcata atttcaataa gcagctgtca gaataaaatt
    11281 tcttttcttt gatttaaaag cttgcaaaaa agagggagaa tgagtattat taaaaatgta
    11341 ggttagcact cgaaggttaa gagtgctaac agattaaaaa aagagttatc ggagggattt
    11401 atcgtgttaa aaccattagg tgatcgcgtc attatcgaag tcgcaaaaga agaagaaaaa
    11461 actgtcggag gaatcgtttt agcttctgct gctaaagaga aaccccaaac tgggacagtt
    11521 gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa aagttcctgc cgctgtaaaa
    11581 gcaggcgacc aagttatgtt cgaaaaatac gccggtacag aagtaaaata cgaaggaaaa
    11641 gaatatctga tcgtcgcagg aaaagatatc atggcgattg ttgagtaaat ttggaagata
    11701 aaaaaataat caaaaataat caatgaggtg aatcaatcat ggcaaaagaa ttgaaatttg
    11761 cagaagacgc acgcgcagct atgttacgcg gggtagataa attagcagat acagtgaaag
    11821 taacattagg acctaaaggc cgaaatgtcg tactagaaaa atcttacggc tctccattga
    11881 tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt agaagatcat tttgaaaaca
    11941 tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa tgatattgca ggagacggta
    12001 caacgacagc aactgttctg acacaagcaa tcgttcgcga aggtttaaaa aacgtaactg
    12061 caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt ggcaacaaaa gcagcagtcg
    12121 aagaattgca taatatctca actgttgttg attcaaaaga agccattgca caagtagcag
    12181 ctgtttcttc gggttctgat aaagttggac atttgattgc tgatgcaatg gaaaaagttg
    12241 gaaacgacgg tgtcatcaca atcgaagaat caaaagggat cgaaacagaa ctagatgttg
    12301 tagaaggtat gcagtttgat cgtggttact tatcacaata tatggtaaca gataacgaca
    12361 aaatggaagc tgttctagaa aacccatata tcttgatcac agacaaaaaa atctctaaca
    12421 tccaagatat tctgccattg ttagaacaaa tcttgcaaca atcacgtcca ttgttgatta
    12481 ttgctgacga tgtagatggt gaagcattgc caactcttgt attgaacaaa atccgtggaa
    12541 cattcaacgt agtagctgta aaagctcctg gtttcggcga tcgtcgtaaa gcaatgcttg
    12601 aagatatcgc tattttgaca ggtggtacag taattactga cgatctaggt ttagaattga
    12661 aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt tgttgtagat aaagacaata
    12721 caacgatcgt agaaggttct ggagaaaaag aagcaatcga agctcgtgtg caattgatca
    12781 aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga aaaattacaa gaacgcttag
    12841 ctaaattagc aggtggtgta gctgttgtta aagttggtgc agctactgaa acagaattga
    12901 aagaattaaa attacgcatt gaagatgcat tgaatgctac acgtgccgca gtagaagaag
    12961 gaatggtctc aggtggtggt acagctctag tgaacgttat cagcaaagtt tctgctgtag
    13021 aagcagaagg agacgtggca acaggtatca agatcgttgt tcgtgcttta gaagaaccaa
    13081 ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt gattgttgat aaattgaaga
    13141 acgtggaact aggcactggt ttcaatgctg ctactggaga atgggtaaac atggttgaag
    13201 caggtatcgt agacccaact aaagtaacac gttctgcctt gcaaaatgcc gcttccgttt
    13261 ctgccttgtt attaacgaca gaagcagtcg ttgcagataa accagaacca gcagcgccag
    13321 cagctcctgc aatggatcca tcaatgatgg gcggtatgat gtaagaaaaa gcaacttgct
    13381 tatctacaac tgatcattag tgaaagacag ataaaaagag gctggaacag aagcgtttaa
    13441 ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca aatttttgtg aatttcagct
    13501 tatttccgaa ggagttgctt ctgcttccgc tgtttatacg tgtttagagc gtgagacaaa
    13561 agtgttcttt acttttgtcc cacgctcttt tttgtgtgta attataatga aaatagttat
    13621 ttttaatagt tataatattt tatctattaa aagtaaatat ccattcacga ttacgtccat
    13681 ataattgtgt aaaagtgaag catcaaaaaa gccattgatc tcagttagga gtacaatgtt
    13741 ttttgcagaa aacatactcg aataggactg atatcaatga ctcaagtaca ttttactttt
    13801 gaaagcgaag aaattcaagc aattataaac gaaagcggtg cgaatgatac cgcaaaaaca
    13861 cttatgacta tcatgtttaa tcagctgatg gaagaacaac gaaatcaata tattcaggca
    13921 aatgcctatg aacgttcaga agaacggcaa tcacaaagaa atggttacta cgatcgttcg
    13981 tttacaactc gaattggtac gttagaactt catgttccac gaaccagaga cggtaagttt
    14041 agtcctacta tctttgaaag atatcagcga agcgaaaaag ctttgatcgc tgccatgatt
    14101 gaaatggtca tttctggtgt ctccactcgc aaagtaacta aaacagttga actgctaacg
    14161 gatggtgcga ctgtttctaa atcattcgtt tcaaatctga tgaaacagtt ggatccattt
    14221 gtttttgagt ggagaaaccg gagcctagaa ggctcggaat atccattttt catgtgtgat
    14281 gccctgtata tgaaagtaag ggaaaatcat cgtattgtct caaaaggtgt atatattggt
    14341 atcggcattg attctgatgg acgacgtacg attcttggtt ttgatgttca agacggcgaa
    14401 tcggaagata attgggatac cgtttttcaa tcgttcgttc aacgaggttt gttcggtgta
    14461 aaactggtca tttctgatgc acacaaaggg ttagttaagg ctgttcgcaa gaacttttta
    14521 ggtgcgagct ggcaaagatg ccaagcccac tttttgagaa atatttttga taagctgcca
    14581 aagaaagttt cttctgatgt aaaagacgag ttgaagagta tctttaaagc ctctgaactt
    14641 gaattgacac gtgaaagaaa agagcacttc ttggaaaaat atggttgtga ttcaaaatta
    14701 agtgctgctt gcgacatttt agaaaacggc tttgaagatg ctattcaaat tttgtctttt
    14761 cctgaaaata tccgccgaag gattcgtaca accaatgtgt tggaacggtt gaacgaagag
    14821 attcgccgga gagaacgagt gattcggatt tttccaaata tcaattccat cactcggata
    14881 attggaacac ttttgatgga aaaagacact gagtggctgg cttctccacg aaaatattta
    14941 gaatttaatt cgaataacat ttagcaccat gaacctgtgc gagtctcttc tttctgagga
    15001 gagacatcaa gagtcgctgc gctactgctc ctgacctctc tcctcagaaa gaaaggcatg
    15061 gcagttaggt tcacaacgca aaacattgtg cttttgatga ggttgaactt ttacacaaga
    15121 ttgtggactt gacttattaa tttattaaaa aaagagaaag aagatttaaa tgatgaaata
    15181 aatcaaaagt atgatgaaac attaaaaaat gtctattctt cactt
//
LOCUS       DAZAID010000069        14966 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000069 DAZAID010000000
VERSION     DAZAID010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.069"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..2382
                     /locus_tag="ACG9BE_002022"
     CDS             10..2382
                     /locus_tag="ACG9BE_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFN3032758.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2443..3903
                     /locus_tag="ACG9BE_002023"
     CDS             2443..3903
                     /locus_tag="ACG9BE_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFN3032759.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3914..6118
                     /gene="topB"
                     /locus_tag="ACG9BE_002024"
     CDS             3914..6118
                     /gene="topB"
                     /locus_tag="ACG9BE_002024"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN3032760.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6134..6283
                     /locus_tag="ACG9BE_002025"
     CDS             6134..6283
                     /locus_tag="ACG9BE_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032761.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6358..6951
                     /locus_tag="ACG9BE_002026"
     CDS             6358..6951
                     /locus_tag="ACG9BE_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFN3032762.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6964..7863
                     /locus_tag="ACG9BE_002027"
     CDS             6964..7863
                     /locus_tag="ACG9BE_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFN3032763.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7866..8363
                     /gene="ssb"
                     /locus_tag="ACG9BE_002028"
     CDS             7866..8363
                     /gene="ssb"
                     /locus_tag="ACG9BE_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN3032764.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8729..8989
                     /locus_tag="ACG9BE_002029"
     CDS             8729..8989
                     /locus_tag="ACG9BE_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032765.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9434..9640
                     /locus_tag="ACG9BE_002030"
     CDS             9434..9640
                     /locus_tag="ACG9BE_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFN3032766.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9661..9891
                     /locus_tag="ACG9BE_002031"
     CDS             9661..9891
                     /locus_tag="ACG9BE_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032767.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9907..10344
                     /locus_tag="ACG9BE_002032"
     CDS             9907..10344
                     /locus_tag="ACG9BE_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032768.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10337..11044
                     /locus_tag="ACG9BE_002033"
     CDS             10337..11044
                     /locus_tag="ACG9BE_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032769.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11208..11408
                     /locus_tag="ACG9BE_002034"
     CDS             11208..11408
                     /locus_tag="ACG9BE_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032770.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11424..11603
                     /locus_tag="ACG9BE_002035"
     CDS             11424..11603
                     /locus_tag="ACG9BE_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032771.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11928..12191
                     /locus_tag="ACG9BE_002036"
     CDS             11928..12191
                     /locus_tag="ACG9BE_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFN3032772.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12185..12535
                     /locus_tag="ACG9BE_002037"
     CDS             12185..12535
                     /locus_tag="ACG9BE_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN3032773.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12559..13173)
                     /locus_tag="ACG9BE_002038"
     CDS             complement(12559..13173)
                     /locus_tag="ACG9BE_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3032774.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13487..13909
                     /locus_tag="ACG9BE_002039"
     CDS             13487..13909
                     /locus_tag="ACG9BE_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032775.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13919..14122
                     /locus_tag="ACG9BE_002040"
     CDS             13919..14122
                     /locus_tag="ACG9BE_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032776.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14333..14917
                     /locus_tag="ACG9BE_002041"
     CDS             14333..14917
                     /locus_tag="ACG9BE_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3032777.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5515 a         2429 c         3037 g         3985 t
ORIGIN      
        1 ggaaagaaca tggcagaaga aaaagaacca ctagacaata gtcgtttggg aaaatccaaa
       61 agaaaactag tacgattgca aaatgaatta aacgaacaaa ttgaaaagat gtttgaacac
      121 caaaaaatga cgaacggtca accaatgaac gacaagagga atggccgttc atggtttaac
      181 cagcaagaac gaatcgaaaa taaagtccaa agtctgagag aagagatcaa gcaacaagaa
      241 gaatacgtgg aaaagcttga aagacaagaa gagctaaagg aaatgggcta taacaagtac
      301 ggcggactgg atatgacgat cgataatatt ccaagaatca aagaagaaat cgaacgcttt
      361 gaaaaaggtg aatcggcttt ttcagccgcc acgatccgaa aataccaacg gaagttggaa
      421 acattagaac agctgaaaga aaaatcagaa aaaggaaaag aaaatatcct ccctgaagta
      481 caagcgatca ttgacagcgg acgagtgacg caatggaaaa aaaatccaac catttatttt
      541 ttgaaaggct atcgaaaagt tgctttagaa ctgaatgaaa acgggggatt tgaggaatca
      601 aaaaaatacc aagccacgtc ggaagaggaa aaagcagctg ttcgtgaatt acttgaagag
      661 gctagaccaa ctgattctat gaaggagaaa gaaaacgatc atttctatcg agtagaattc
      721 aacgaaaatg atccagaaaa aggattgaaa agttatggtg gggagatcgt aacattcgaa
      781 ttgatcgatc aactcaaaac attggataac caatacgccg cagaagcagg ttattataaa
      841 ttctattttg aggaagtgaa gaacggagaa gtagtcgatc agatccgaat ggatctaggg
      901 gatggcgtca acagtaatca accaatctat ttagagttag caaaattgtg cgaacaagta
      961 cctgagaagg aacaaaaaca atcggtcaaa aatgaacctg tttcgaaaga acaaatgcag
     1021 tcgaaagaaa caaaaacaaa acaaaagggc tatcaaaaac gaacggcaga agagattaaa
     1081 caagaagtta aatccctttc gcaacaggca ctggatgcgg tgaaaaagca tacccattct
     1141 cctgaagacg tcaaagaact tttgaacttt atgtctcgtt ttccagaacg atcctttaga
     1201 aatcaattat tgattgagga acaatttcca ggtgcaacgg cttgtttagg tcgtgccgcg
     1261 ttgaaaaaag aaggaatcta tatcaaaaaa ggggaacaag ggaacaagat ctttgtcaga
     1321 aaaacggtca aagggtttta tgagaaagga cgtggctttg ttcgagaaac agaagctaca
     1381 ccagaagata aaaaacgaat cgaaagtggt caaatcgaag tgatcaaaaa accttactat
     1441 acgattgaaa aagtgtttga tatcacacaa acacagttaa aacctgaaga ctatccgaaa
     1501 atctttccga accgtgtctt tgattttcaa ttggataaaa aaggacagtc agaacttcaa
     1561 gctgggattc aagcggtagc caacaatatt ggaatacaaa ttcgagatat gacagaaagt
     1621 gaagtctatc aaagagaatt aggacaagca cgtggggcgt atgttcgtaa cgaatttact
     1681 ggcgaagaag aaatcgtcat gaatactcga aatactccta cgcagcattt agctacaagt
     1741 attcatgaat tagcccatgc aagaatgcac aaattttcag aattagatac cgcaactaaa
     1801 gaattgcagg cagaaatgac ttcttatatt gtgtgcaaac attttggaat ggacacgtcg
     1861 gagaaagcta ttccttatat tgcagcatgg acgaagaatg gtcagacgtt ggatgataag
     1921 gaagctgcgg aacgtgggaa aatcatgaat gatgtcagcc gtgtcgcaaa tgaattcatt
     1981 caaaccattt ctaatgaaat caatcaatac agagaaagag aaccagagat ccaaccagca
     2041 gaagtgaagc aggagcataa aaatacttta gaaacgccag agaataagaa tattgtgccc
     2101 gttggttcac tttggatcga caagaaagac gggaaagtaa tggaagaaga tacgggtcga
     2161 acgttacatt tgaaggatgc ggcgtttatt gaagatccgc agcataatca agtaatttta
     2221 aaaggaaaaa gttatggaaa agagatccaa acggcgttac cttcagaaga atttgcgcta
     2281 ggaaatccat tccgattttc acttgagaca gtgtcagaag tggcaaaacg aaaaaatgaa
     2341 atctattatg aacaacaatt gtcacaaaaa gaacgtgtat aaaacaagta aaaaagacta
     2401 tctgaaaaac ggatagtctt ttttcttttg ggaggtttta aaatgaccga gaaaaaaatg
     2461 agtcttattg atcgatgtaa acagatcgat atagtggatt ttgctcgaaa caatggaatg
     2521 gctgtagtca ataaaggacg agattatcgc ttagaagacc atgattcatt tgtttttgat
     2581 cgaagaaaac aacgttttta ttggaacagt caaaatatta gtggcgacat tattgaatta
     2641 gctaaacttt ttttcataga caaagagatc caagacccta agcaacaatt taaagcagcc
     2701 actgatttta ttctaaagaa tgaagacaaa acagaacgag tggaaaatct tcattttgaa
     2761 acagaaaagt acaaagatca ccccgttgat tatcaaccac tcacagaaaa aggaaggaat
     2821 tatctgaagg aagaacgaaa actaccagag tggctgattg attatgcaga aaaagaaggg
     2881 ttgattgccg aactaaagcc aaaatatgaa cggcaaaatt tcttagttcg agacgatcgt
     2941 ttagatcatg cggtcgcctt tttatggaaa gatccacaga caaaagagac agtaggggcg
     3001 agttatcaag gaacgttcat tgattatgaa cgttttggtg agagaggcac atataagcat
     3061 atagataaaa attcgacagc gaatcatggc tttaacctaa agatcggtga ccctaagcag
     3121 ataaaatttt ttgagagtag tattgattta cttagctatg ctgcactgaa tcgtgatcag
     3181 ttaaacgata cgtggttagt ctctatggaa gggttaaaac accatgtgat cagtcattat
     3241 tttggtgagg cagtatctga attgagaaag aaacaagcct ttcctcaatc catcgagatt
     3301 tgtgtggata atgaccgtgc aggacatatt ttctacgaaa aagaacaatt aatgggcgca
     3361 gtcgatccat ttacgaatca gaaagtacgt tgtgaacgag gaatcgctaa tgattggcaa
     3421 gtgccaaaag aatacaaaat catttatgaa gaagtcgcaa aagaagaaaa ggtcacacca
     3481 gaagctatta tggccataca caagacagaa aacaatctgc aattaacgaa ccaactggtt
     3541 tccgctcaca aagtaaatgc gttttttggc cagcagcttt ctgttaacga ttcaatagaa
     3601 gccattaatt tgaaagatat ttgtagagaa gtcgcaaaag aattaaaagt ttgtgaacgc
     3661 gtggatggta cgtacgattt tgatcgtttt tatcaagaaa aaggggatat taatgcgcag
     3721 attcttttct cttataaagc agaacaatac tacaaaggct ataaaaatca tgaacatgaa
     3781 tttgtacctg aagtaaaaaa ggactggaac gatcaattga agcatgagat ccagcaacaa
     3841 gaaatcagaa agcaaaaacg tgccatgttg tttcaacaag gcagacaaca agaaagggag
     3901 tgaaatcgtc atgatgaaaa ccgttatttt agcagaaaag ccgtcacaag cgaaagctta
     3961 tgccgatagc ttctctaaag ccactcgaaa agacgggtat tttgaaatcc aagatcgatt
     4021 gtttcctggt gaaactgtga tcacgtatgg ttttggtcat ttagtcgaat tagattcgcc
     4081 cgatatgtac gatgaaaatt ggaaacaatg gtcattagaa catttgccta ttttccctac
     4141 acattatcac tatcatgttc ctaaagataa gaaaaagcaa ttcaaggtgg tgaaacagca
     4201 acttcaatcg gccgacacca ttattattgc gacagatagc gatcgtgaag gggaactgat
     4261 cgcttggacg atcattcaac aagtaggcgc cgatcaagga aaaaccttta aaagactttg
     4321 gatcaactcc ttagaaaaag aagccattta tcagggattc cagcagttac gagacgcaaa
     4381 agagacctat ccaaaatttg aagaagcaca agcacgtcag atcgccgatt ggttaattgg
     4441 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagctt
     4501 ttccttaggc agagtccaaa ccccaacgct ttacatgatt tatcaattgc aggaaaagat
     4561 tagaaatttc aagaaagaac cttattttga aggaaaatcc gaagtaatcg ctcaaaatgg
     4621 cgcatttgat gcgaagcttg atccgaatga aacccaagcg acgcaagaag catttgaaga
     4681 ttatttaaaa gaaaaggggg ttcaattggg aaaacagcca ggcatgattc gccaagttga
     4741 aaccgagaaa aaaagcgcgg caagtccacg tcttttttct ctatccagcc ttcagtctaa
     4801 aatgaatcag ctcatgaaag ctagtgccaa agacactttg gaagccatgc aaggcttgta
     4861 tgaagggaaa tatctaagct atccgcgaac ggatacccct tacattacgg aaggggaata
     4921 tgcgtatctg ttggatcact tagacgaata caagcatttt ttgagggctg aagaaatttc
     4981 gactcctatt catacaccga atagtaggta cgtgaacaac aagaaagtcc aggaacatta
     5041 cgcaatcatt cctacaaaaa cagttatgac agaagcgacg tatgaaagtc tgacaccttt
     5101 acaacaagcg atttacgagc aagttttaaa aacgacagtg gcgatgtttg cggaaaaata
     5161 tacctatgaa gaaacaacga tcttgacgca agtccagcag cttcagttaa aggcaatcgg
     5221 aaaagtaccg atagatctag gttggaagaa gttatttggc aaagagagcg acggaaaaga
     5281 gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga cggtagatct
     5341 acaagtcctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta cgttgatcac
     5401 tgcgatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca tattgaaaga
     5461 agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct tgaaacaaaa
     5521 ggaatatatc aaggtagaga aaaataagct tgtggtgacg aataaagggg ttttgctttg
     5581 ccaagctgtg gaaaaagagc cgctgcttac gagtgccgaa atgaccgcca aatgggaaag
     5641 ctacttgtta aagattggag aacgaaaagg aacacaaacc acttttctag ccaatatcca
     5701 aaagtttgtc tctcacttat tggaagtcgt cccaggacaa atccaatcca ttgattttgg
     5761 atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc gtcatttagg
     5821 aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg cagcttgtac
     5881 gtctgaagcc tgtgatttta aattatggac aacgattgcc aaaaagaaac tcacagcgac
     5941 acagctgaag gagattattc aaaatagccg aacaagccaa ccagtgaaag ggttaaaagg
     6001 tcagaagggt tcgtttgaag caacgattgt tttaaaagaa gacttcacta caggctttga
     6061 attttctgaa aagaaaaaaa cgaacttcaa gaaaagaaca cgacgcacga caaaataatg
     6121 gagggttaat cttatgcgta atacaacaca caaacaacaa gaaatcgaaa atcaaaaagc
     6181 caaaattcgt tcagaacaag agaaattacg tgccagtaaa agaagacttc agcaattaaa
     6241 gaaccagcaa cggattcaaa aagaaatcga attagatcga tagacattaa ccttgttaca
     6301 aatacactat catgatatat ttaatgtagt gtatttgtaa caaaaggggg gaagtaaatg
     6361 caagagaaac tgaaagaatt gttggctatt cgcgatggaa atttatctat gaaggaagcc
     6421 agaaaattag gaatcgcagc aaccactgta caaagattgg tgaatcaagg aaaattgatc
     6481 aaagttgatc gtggttatta tgtagaggat ggtcatggaa tagatgattt attttggctt
     6541 cagcaacgtt tttccagggg aatcttctct catgaaacga ctttagatat ttaccagctt
     6601 tctacgaata tgccgaaatt cattcactta acttttcccc atggatataa cgttgatcgt
     6661 tcaattacaa aagagcagca gttacaattt catttcgtga agaaagaagt ttatgaacta
     6721 ggaaaagtag agggaacttc ctttcaagga aatcctatta ctatgtatga taaagagcgt
     6781 acactttgtg atatttggaa tccgagatat gaaattgaat atgaaatgaa attagaagca
     6841 ttaaaagagt acatggaaga cccattgaga gatccaagta aattaagaga ttacatgaca
     6901 aaactacatg tagaaaaaga aatgaaatac cacatgcaaa gcttatattg atggaggaaa
     6961 aagatgacac cgacacaatt tgcagcaaaa acaagaaatt tagcaaagag gacaggatta
     7021 aatgcacagt tagtacaacg gcattttatg atggatcgtt taattgaaca aatttctgaa
     7081 tctaagtata aaaatgattt cattttgaaa ggtggctttt tattaggatc aaaatatgga
     7141 atcgatagaa ggacgacaat tgatatcgac acgacattta gaaatagcaa attaactgaa
     7201 gaaaaattga agagtatttt taatgattta actaggaaac ccacgaaaga aggtattgtt
     7261 ttctctattc aaggaatgaa ggaaacaaga gaagccgatt tttatcctgg atttcaaatt
     7321 aaattggtgg ctaatttaga gaaagtacgt gtgccattta aaatggacgt gacaacagga
     7381 gattctattt atcctgaagt agatacacac tatcaccaat taatgtttga agataaaaag
     7441 gtggagattc cagcttatcc aacagcacaa attattgcag aaaagctaag tgctacgttc
     7501 agctttggca cagataattc gagagcaaaa gattactacg acttatttac cattccgaaa
     7561 ctagaaaata tcgatacgaa agagttatac aaaagtgttc gaaatacatt tacaaaacgt
     7621 ggggatacaa aaccgttgtc cttttattat gaaagagaaa tggataagat cataggaaat
     7681 gactatttgg aaaagcagtg ggaaaaatat cgctcagtta acacgtttgc gaattccatt
     7741 tcatttacag atacagtcaa cgaaattgat cacctaatga aaagtgtgat caaaattgaa
     7801 aatcaagaaa gaaaacggca gtttaaaaga tcacaacaaa tgaatcaaga aatggagaga
     7861 taaagatgat caataatgta actttagtag gacgcttaac caaagatcca gatttacgtt
     7921 ataccgcgag tggtactgca gtagcgactt ttaccctagc tgtcaaccga aatttcacga
     7981 atcaaaacgg caatcgagaa gctgatttta ttaactgtgt gatttggcgc aaaccagcag
     8041 aaacaatggt gacattagct aaaaaaggta gcttgattgg tgtagttggg cggattcaaa
     8101 cgcggactta tgacaaccag caaggacaac gtgtgtatgt gacagaagtt gtggccgata
     8161 atttccaact attagaaagc aaagcagcga cagaaagccg tgcgcacgct gatcaatcgt
     8221 ccaccagtcc aagcaccacc acttttgaac aacgggatac agcgacgcct aataacaatg
     8281 gattgaacgc tttacagaat ccatttggga atccatttgg tggacaatca atcgatatta
     8341 gcgatgatga tctaccgttc taagtagcat tttctattgg ttgcatttgc tacggtttat
     8401 gttataattt agtcaacaaa aagaattttc aatctataac caaaaaccac tagctggcgg
     8461 gctagtggtt ttcttttacg tttctattat aagaattaac gggtaggctt actgtctttc
     8521 ttggcaaatt tacaatcaag ccatttgcag atatagtgcg cggctacttc tgccatgatt
     8581 gcaaaaacaa aatcaaaaag aattttcaaa cctataacct cctccccttg ccagattagg
     8641 ccggggtagc agacagcaag actattatag cataattgaa atatccaaat gattggattt
     8701 ttgctataat tttagcgagg tgattgtgat gaacaaatat gttggtttgc ttgataaaat
     8761 ccgagtaata aaaacgcaac cgttattggt tcgctttact ttacaaacga ttcatgaatc
     8821 gattaattgt gtagttgcag atattgaaat tatcgataaa ttgctaatta tggatgatgg
     8881 aaaatacaat attgccgtca caggtcactt caataaacga aatcagctag ttattgctag
     8941 catgtatgtt agaaatcccg atcattttac gcgatcgatg ggcatataaa aaaagaagag
     9001 ttgcaatcct aatgtaggac tgcagctctt tttgttttac atagaaacaa gaaaaatgaa
     9061 aaggcctttt taggaaggcg cgagcgcctt ctcaattgaa aaacctctct cactccaacc
     9121 gccaccgccg ttcgagaaga aaggaagtga acgttgcacg ttcactaaga taaagggtct
     9181 tgcaaagcaa gacaaaatag aaaaaacttc ctctcaccaa aaggttcgtt cctgttcgtt
     9241 atctctttat tgatttgcca gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa
     9301 ggacaagggt aaatggcacg ctgcgcgccc ttgtccttgt cgcttttcgt tgattcattc
     9361 tggccaacaa atcaataaag aaagaactaa cgaaaggaag taaaaccttt cctaagagga
     9421 ggcagaatca atcatgcgaa agaaatttga ttattggggt gttcccttca gcgaattatt
     9481 cccaaactat catgcaccac atactgtcga atgcgattgt ggacaaagag cgaaatgtat
     9541 caaaagctat cgtttgtatc aatgtccaac atgcggaaaa aaatacacct tgtcttatgg
     9601 cgattatatt ttgattgatg agaaaggaaa gaaacgctga tgaaaacata acataaatta
     9661 ttgactgtaa aagaagtcgt tcaaattttg gtggacaaag aagtcatttc tgaaaaagta
     9721 gcagaagctt tacttattcc gcggtactgg acgatagaaa cccctttagt agaggatgca
     9781 ccgactatgc tatttgaaga cctagtgaag attgcaggat atgaactgca ttatcaagaa
     9841 aacatggaaa cttatgaaaa cgaatattgg ttaaccaaat tagagaacta aaaaggagaa
     9901 aaaaatatgg ctcaaaatta ctatgacgaa tttgtcaaat tacctttgga taaaatggca
     9961 caaaaaatgg aggatatgac attcctatac catgagacac gtgttccaaa aaaacattat
    10021 aaggaaagat tgtcggtagc tgtggaagaa atgattgaat caggtgtcga aatgaatctc
    10081 attgcgacgt actatcgaac actggaagaa ctgaaaaaac aaaatgcaaa atggtttttc
    10141 caagccttac tatgcttgga agtaggtgtc aaaccaagca ctattaaacc ttctgaatat
    10201 caagctttag aactaacgta tgggaaattt gtcgaaacga aaaaagccaa gactgtttca
    10261 agtgaatggt tagattattt tgaaaatatc aataagtatg gcgcctatta caccatgaaa
    10321 aaggaggaca acgaaaatga ataaggtgat ttatcaacta acaacggaag atgtgtcaca
    10381 ctttttaaca aacaaagaac atttgaatga agtaatcaga cggattcaaa aagactttag
    10441 ttgtatggaa gaaatgaagt actttattca aagagtcgaa gaacaattgt atttggaaag
    10501 tgaaacgagt caaacgaagg tcacagtcag caataattat ttagacgtgt ataaacatcg
    10561 tcctgaaacg gtaaaaaaag gaacggagct tctacgacta gaaacagaaa aatacatcat
    10621 gaatgtttac ctagaaagga gtgaactggt cgaaaacttt gtcagctgca cgatttgcag
    10681 gaaacactat tactcagaat cagaatatta ttcaattttt gagagcatgg aattaatgga
    10741 agaagaaatg gaagagctaa acagcaaaaa tgcgacgata gacgattatt atcttgtatt
    10801 tactagattt ttgacgtgtg gaacggaatc tatcgtgtta atgggaatgg attatgaact
    10861 cgctgtaccc gaagcatttt tgagggaata tgtagaagaa gtagataacc caggcgacga
    10921 ctggtacacg actttaactg attttttaga tacctacact tacgacgata cagaagaaat
    10981 ccaaaatgaa cttgaccgta aacgaatgcc atatcaacga tttgaaagga aaaaaaggca
    11041 ataatcattt ataccgaaaa gtgagaatgt gtgaaataag aacaaaaacc ttgaaattaa
    11101 gggaatggtt cttatttcgc accaatcgct ccaaccgccc ccgccgctta ggtggtgcga
    11161 aataagcggg caaagcccga taacctaaga gaaagaagga gatccatatg aataaaatca
    11221 ttcctctgtt gttcgtactg tgtattggcg agatacttat gcgtcatggt gcagctgtac
    11281 atttaaactt cgtagtattc ttaggaatac tagtattttg tttgattttt tggcgctttg
    11341 tgttagccct tgtgagtcta ctattcaagt tgattgcagc catatttttc atagggttat
    11401 ttcgatgaaa ggagagagta ctaatgccaa ttgcaatagg aaataaacgg ttacccgtga
    11461 cattagatga aaagagacaa aaagaattgc agcaactaaa gcagaagtac ggaaaaagtg
    11521 aatctaagat tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg
    11581 atataccagc actaaaaaag tgacgtcact tttcatttga agaaacaaaa gtgatagcac
    11641 ttaattgttc ctactattta gaatattttc tatgtccttt ttattccatt ttgtatcaat
    11701 aactatgaaa tctttaaagt atccagatca atttttttct tacctgattc taaagatttt
    11761 gttttcttaa acatattatg atttcctttc gactattttc atataaatgt taacatagtt
    11821 tatggattca tggaatgtta atctaattta atagaaaagg ataattaagt atatacgttt
    11881 tttcttatgt atgttatact atgtatatac agaaagaggt gtctactatg gaaatgttac
    11941 ctataaaaaa atggggaaat agtaatggcc ttagattgcc aaagtatatt atggaatatc
    12001 ttggaattca tacagaagac aaagtgaaga taatacaaga agaagtaaac ggtcagaagc
    12061 ggctaattat cgaagcggct aatatagaaa atgaattaac aattgaacag ttatttgaaa
    12121 attacactga tgaaagaaat catgtgacga ttcaaaattt aggtgaagca gtaggtaatg
    12181 aaaaatggtg agaaaaccta gacaaggcga tattctatta ttgaatacag cacctcgatc
    12241 tggacacgaa cagacaggta aaagacctta tattgtttta agtcatgata ttattgcgga
    12301 ttatagtaac gttgttacag ttgctccaat ttctagtaca actagaaact atccgctata
    12361 tgtaaatata aactcagaat atgaaatgaa gacaacagga aaagtattat tagatcaact
    12421 aacgacaatt gattatgaag ccagagagtg tatttttttg gaaaaagcgc atgatagttt
    12481 agttgaggag ttattgatga aagttagaac agtttttcaa aaagtaagca aataaaatag
    12541 aaacagcagc atgatcaact attcgtgatt gaccatgctg tcttttttta ttctatatac
    12601 ggtttgtctc gtaacattgt agtcttttgc aatcttagcg atcgcaacac ctttttttag
    12661 atcctcaacg atgtttttat aaacaagacg acgttgggga tcttttgcat tggcactgta
    12721 tagcttagga cgtcctttat aaacaccgtt cgcttttgca atcttaatgc cttgtgcctg
    12781 acgtcgttta gattcggtac gttcttgttc agcgatcatg gccaaaattt gaatgatcaa
    12841 atctttaatg aatttatcca ggagaggatt gccaattgct tcggccatga tcggtagact
    12901 tgtgatgatc agctgcacat ttttcttctt caagtaattg actgaagcga tgatctcgtc
    12961 gtaatttctt ccaagtcgat caatagcttc cacaataaaa atatcttgat ctcggacgaa
    13021 atccagtgct tcttgaaaaa caggtcgatg agtaatcgtt gcgccagatt gtttctcaat
    13081 gaaaattctc tctgcaccga attttttcat ttcttcaatc tgccgttgtt cattttgatc
    13141 gatggatgag acacgtatgt atgcaactct caaaattttt acctactttc tgcaactatt
    13201 taaaaaacac cctaatcata cacgagaaaa ccagattttt caatgtcgcg gtcgtgtcta
    13261 gttagggtat accttatata gacaaaaact aatgatggag gaaatagtat gtgtcacttt
    13321 tctttttaaa tggcaatatt ttttgtaaat aaatgtcaat caagaaaatt taaacgatta
    13381 tgacattaaa aatatatttt atgaattttc tagtatataa cttgatttat ctgttaagat
    13441 ttattaagaa aacgttttct tatttttgat ttcgaggtga tagacaatga aaaataagtt
    13501 tattttatct ttaggtattt taactattgg aattagtgta actggaacta caattcctac
    13561 atttgctgat agtacaaatg aaccaactac tgtagtagat aataaccaag aattaactcc
    13621 tttagatagg aacggaatag ccattggtaa cccaactaac gacttaactg ttactgaaaa
    13681 ttatttaata gtttctccat tacttgctcg agcagcttct aaaacgatac gagtgacagg
    13741 aacgatgatt tttgataaag ttggttggcc ttctagacct cctcaatcac ggaattggaa
    13801 agaaaaaaga aatggttatt ggtactatgg aaaattgtat ttacaaagtt ataaatcagc
    13861 tggaaacaat tggattgcga cttattcagg aaccttaact agaaagtagg gattttttat
    13921 gccagaattt gtgaacacac caaattttta tcaagatagc gatattctaa gtaaaattaa
    13981 tttaccgaaa gaagaatcag ctttacaagt tcagcgaact ttacaggaga cacaaaaaat
    14041 tcaaaaatca atttctaatc tagaagattc tgaagaaaaa agaaaaaaaa gagaccaaaa
    14101 aacatatcaa aaagaaaaat aatgatttta aaaaaagagt gtctagtcga cattcttttt
    14161 ttctaattga aagtgttcat aaaatcaccc atttctggac actttctttt tttgctttat
    14221 ttttcaaaaa aacaattcat ttcatgttca taaacccata acaatatcta tgttcagtta
    14281 aactatatgg ataaagttat gttacaataa aattaatgaa ggagtgattt ttttgaaaat
    14341 aggatacgcc cgggtatcta ctggtttaca aaatttggat ttacaagaag acagcctaaa
    14401 taaatttggt tgtgaaaaaa tttttactga tcatatgagt ggggcaaaaa gtaatcgacc
    14461 aggattagaa atggccattg attttgttcg ctctggcgat acacttgtcg tttggcgctt
    14521 agatcgttta ggtagaaata tggaagattt gatttcaatc gtcaatcgac tgaatgagcg
    14581 tggtgtgagt tttcatagta ttcaagaaaa tattacgatg gacaaatcaa gttctacagg
    14641 acagctcatg tttcatttgt ttgcggcatt tgcagaattt gagcgtaatt tgatcctgga
    14701 acgatccgca gcaggacgag cagctgctcg tgcaagagga cggtttggtg gtcgtcctga
    14761 aaaattgtct gaacaagatt tggaattact gaagacttta gtggatagcg gtacacctat
    14821 taagactatt gcggaacggt ggaacgtctc aagaaccacc atttatcgtt atttggcaaa
    14881 aatggaggag aaaaatggaa agacaaattc tcattagttt ttgtcattta atgctgaaaa
    14941 tttttgccat ttatctagaa aagtga
//
LOCUS       DAZAID010000070        13935 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000070 DAZAID010000000
VERSION     DAZAID010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.070"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..432)
                     /locus_tag="ACG9BE_002042"
     CDS             complement(<1..432)
                     /locus_tag="ACG9BE_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032778.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYG"
     gene            398..2035
                     /locus_tag="ACG9BE_002043"
     CDS             398..2035
                     /locus_tag="ACG9BE_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032779.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2180..2920)
                     /gene="truA"
                     /locus_tag="ACG9BE_002044"
     CDS             complement(2180..2920)
                     /gene="truA"
                     /locus_tag="ACG9BE_002044"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFN3032780.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3111..3746)
                     /locus_tag="ACG9BE_002045"
     CDS             complement(3111..3746)
                     /locus_tag="ACG9BE_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFN3032781.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3893..5113)
                     /gene="sstT"
                     /locus_tag="ACG9BE_002046"
     CDS             complement(3893..5113)
                     /gene="sstT"
                     /locus_tag="ACG9BE_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFN3032782.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            5332..6330
                     /locus_tag="ACG9BE_002047"
     CDS             5332..6330
                     /locus_tag="ACG9BE_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032783.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6526..8361
                     /locus_tag="ACG9BE_002048"
     CDS             6526..8361
                     /locus_tag="ACG9BE_002048"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN3032784.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8421..9137)
                     /locus_tag="ACG9BE_002049"
     CDS             complement(8421..9137)
                     /locus_tag="ACG9BE_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFN3032785.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(9364..9906)
                     /locus_tag="ACG9BE_002050"
     CDS             complement(9364..9906)
                     /locus_tag="ACG9BE_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFN3032786.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9911..10138)
                     /locus_tag="ACG9BE_002051"
     CDS             complement(9911..10138)
                     /locus_tag="ACG9BE_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFN3032787.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(10217..12097)
                     /locus_tag="ACG9BE_002052"
     CDS             complement(10217..12097)
                     /locus_tag="ACG9BE_002052"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN3032788.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(12286..12900)
                     /locus_tag="ACG9BE_002053"
     CDS             complement(12286..12900)
                     /locus_tag="ACG9BE_002053"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3032789.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            13042..13602
                     /locus_tag="ACG9BE_002054"
     CDS             13042..13602
                     /locus_tag="ACG9BE_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3032790.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         4385 a         2681 c         2189 g         4680 t
ORIGIN      
        1 tccataaaaa acgactgcag gaattattgt aagaaaagga taaaaatagt tcagtccatc
       61 tccataattg ttaaatgttg tatagttgat tggcccttca aaaatagaag ataacccttt
      121 gattcgtgaa agatgaaaag ataaatcacc tgttgtataa atttcttgag catttctcat
      181 aaaatttact gaatataaag tactaagtaa gataagaact actaaaagaa atgctttttt
      241 acttattttc atagatttgt tgttctcctt ttttaattct attttcagta tactagaatt
      301 gtcttttttt aataagtttt gcgttaaatt tttcatagca ctagtatgat atactagaaa
      361 agaatcaatt tttattactt ttaaatagga gaaaaaaatg aaaaaaaaca aaaaccaaat
      421 aatagatatc atttttatgt tatttctaag ttttgcttac gtcatccctc tattcatgtc
      481 taagggaatc tatcatagtg tcaatcagga tacttacttt catttatctc gaatcatagg
      541 actagataat gtatggagtt ctccagtaaa cttcaataac ttcgatcatc atggaacaat
      601 gatgaatatt ttttatccat ggcttactct ttatccagct tttttatttt ataaaatgat
      661 gggaaatctt gttttaagtt ataatatcta ctattttttt atcacttttt taacaatggt
      721 cgtttcttat ttttctatga aacaaataaa aaacaatcgt tatatttcgc tgttattttc
      781 gattatttat acattttccg cctatcgtgc cattgacata ttccatcgtg cttcattggg
      841 agaagctgtc gctttaacat ttttgccttt gatactaatg gggtgttacg aaatttatat
      901 tagagattac caaaaatggt actggttgtc tattggcatg acgctagtag tgtatactca
      961 tttactttct gtcgctatgg tttctgtatt tattggagga acgttgtttc tttcttttta
     1021 tttttgggac caaaaaatcg ctagactctt aagtctttta aaagctaccg tcctcacctt
     1081 ttttttaagt gcgggattct taattccttt tattcaacaa tctagagcac aagaattgaa
     1141 agtcccactg ggaaaagaat taagtggtat ggctcctagt gatatgctga cacatatttt
     1201 aaataataac tataacaact atactattgg attattctta tttctcggat tgattggtgc
     1261 ttttattttt ataaaaaaat taactgctga tgatttattt attttctttc ttggtgtatt
     1321 tgttcttttt tgttcaacca atttatttcc ttggcaactt tttaaccata caccagttaa
     1381 gagtctacaa tttgtctggc ggttaaatgg tttttcttct ttatttatag cctatacgat
     1441 gagtatagtg atctattata ttttttctac taaaaataat tcatggaaaa taatggtctt
     1501 tacgtttctt gcactaatat tgcatctctc aggagtaagc aattctatcc atgctaacaa
     1561 agattcatta acacttatag cacctgaaga cgctgttgct atagctgaaa attacaatca
     1621 tactgattat gcaaacaaag agtcaatcta tcatcctgat atgattaata atgatcaata
     1681 tttactaggt aaccaggaaa taaatccaac aactcatttt atgagtaaca aacttacaat
     1741 tgaattagat aattcgaata ataaaaatac tgtcttgacg acacctattt atcgttataa
     1801 agggcaagtg gcttctatta atggcaaact agtacaaaca aaattatcta agtttggcac
     1861 aactgaatta actattcctc caggtataaa taaagtcgtt attacttatc agtacacaaa
     1921 actagcaatt gcttcaagat atttatctat cgttactttg attttatttt tactttatcg
     1981 atttactttt tctaaacaaa aacaaccgag aagagaagta caacattctc actagatttt
     2041 tatttaagaa agacttttta gctgtacaca taaacaggct aaaaagtctt tttttatatt
     2101 tctttaattt gcttcataca ttatttaaca tcgatattta tactgtgaaa catgcgtttc
     2161 atccattatc aaagtatttt tatcgataat aaacttcatc caaaaacaga ccttgtgcag
     2221 ggacggtttc tcctgcatgt tgccgcactt tgctttcaaa tatttcatcg attgtttcta
     2281 tcggcatggc acctgtgccg atttctagta atgtccccat gataatccgc accattttat
     2341 ataagaatcc gtctccgaca aaagtaaaat gaagcatttc tccctttcgt tcgatagata
     2401 attcatcaat cgtacgcact gtggattttt ttgatttttt caatgcagaa aaaccaatga
     2461 aatcatgtgt acccactaat ttttcgcaag ctgcattcag cttgtccata tccaactctt
     2521 gagggaaata aaagctgtgg tttcgctcaa atgctgaagg aatcacgttg ttccaaacat
     2581 aatagctgta ttttttgccc accgcattat atcttgaatg gaagcgctca ggcatttctt
     2641 cgattctttt gatgacaata tcccgcggaa ggtaacgatt cataaagtcg atcatttctg
     2701 ccagtccaat ttctgtgttt gttttaaaat ttgctacttg tcctcgcgca tgggtgccag
     2761 catctgttct tcctgatcca ataatttcta tctcttcatt tgtcatctga tgcaatactt
     2821 tttcgatttt tccttgaatg gttttatcgt catctccaag tctttgccat cctgaatatc
     2881 tttttccgtc atattcgatt gtcaacttga tgtttctcat attttagtgg ctcctctgtc
     2941 ttccatttgg tcttctctct acctttttaa gattgtagca caaaatatag ctccttgctt
     3001 ctctaagaca gaagctatac aagaaaactg tggaaatata tatttcagaa aaacaaaaaa
     3061 cagccaccgg atgaagtatc cgctgactgt tcaatttgac ggctcaagtt ttactgtttg
     3121 agatgcaaga tactcaacca ggcaatgggc acataattaa agaaaaaact gattctttgg
     3181 caaaaaccta ttttattcag taatggccaa gaatttatag aaggaattct tgccagtcca
     3241 taaatcgccg caaaaagcaa actgatcaca gacagtccaa ggtataagtg gcttacgtcc
     3301 atttgcccat tttttcgata gatcaagaaa agaagaactg gaaacaataa aaaaccactg
     3361 taacctaaac ctgatccgat attatggacc catgtcgaga cattccacga agattcatgg
     3421 gtattgatac tgaacaaacc tgtaaagata caatcaccta taccatagaa cgcgatggct
     3481 gctgtaacta gggtagctaa cacacgagat gtactaacaa atgcagcata caatgcaggc
     3541 aatgacagga tgaaaaagat ccctgaaacc acagaccaaa taagaaacgc ttttcttaca
     3601 ggactggaaa cctctccaaa tacactgatc acgctcttca tttgatctaa ttcaggataa
     3661 tacaatccta atacgtacgg agtcaaaaaa tcactcagca cgcctaataa taaaaagaaa
     3721 aaaccaaatt tacgtaaaaa atccatgata cgcctccaat aacaattacc tatgctttta
     3781 attgttatta taaacgactt tatcttgatt taacagaaac ctctatttac acgaacatct
     3841 atataactca ttaaaaactc gaatcatttt aaaaatacta aatatctaac gttcaacgtt
     3901 cacttaatcc atctaaacaa tctttcaatt tgattttttc cccacttctt ttacgcgcgc
     3961 ctagctctcc tattgcagta aacaacagat cacttgacga atttaaggca gtttccattg
     4021 aatcctggat aacaccgata ataaatccaa taccgacgat ttgcatcgcg acatcattag
     4081 agataccaaa caaactacat gctaatggaa tcaataacag tgaaccgccc gcaacacctg
     4141 aagctccaca agcagctaaa gcagacaaaa tacataaaag gactttcaaa acaaaaggtg
     4201 cttcaatccc taaagaatgt acagcagtca acgtcatcaa ggaaatggta atcgcggcac
     4261 cgcccatatt gatcgtagca cctaacggga tcgatatgga ataagactct tttgtcaatt
     4321 tcaatctttc agccaacatc atgtttatcg ggatatttgc ggcagaactt cgggtaaaga
     4381 aagcgggaag tgcgctttct ttcaaacaaa agaaaatcaa tggataggga ttttctttaa
     4441 taaacaaaaa cgtgaggaat gggtacacaa ctaatgcgac aaacagcatc gtaccgatca
     4501 aaacaagtaa aagctgcaga tactctgcca atccagcgat tccagtcgta gcaattgatt
     4561 gataaaccaa tcctaaaatc cctatcggag caaatgcaat aatcacttgt acgacttgtg
     4621 acaacgctat agatatctga tccaccaact cttttgtcga ttctgaacgt gctcgaaaag
     4681 ctagtcccaa caatacagcc cagaataaga cgccaaggta attcgcatca ataagagatt
     4741 gaacaggatt ttgtgtgaca ttcgtcaaca tagtagataa aacactttcc aaactttgcg
     4801 gtgctttttc agatactgtt tcttgtaaag taataggaac agtaaaaatt gaacttacaa
     4861 taactgctac aagtgcagaa agcaatgttc ccactaaata taagatcaaa ataggcttca
     4921 cgaatgtctt attcccaatt ttatgcttgg caattgacgc catcgtcaag aaaaaaacca
     4981 gaataggtgc gattgccttc aagcaactga caaacaacgt tcccaatatt gaaataaatg
     5041 tccatgtggg gaaaaagata ccaaacaata ttccgactaa aataccaaca acaatacgtt
     5101 gaactaacga catatccaaa aacttctcta acaaatgacc accctttcac aattttaaca
     5161 atcattgtat cattttatgc aaagaaacta aaggtataat tttctaaaaa ttctaacaat
     5221 acaaacaacc atttcttgct aattttgtac tttttctgtc tacacgttgt tttttgagat
     5281 attctaagtt aggatgttat taatgggcaa cttatcagga aacgttttct tatgtttttg
     5341 tccaattttt ttgtgaggtg tttattcttg aaaaaaataa agaaggagag ttatggcttc
     5401 ctgacaatct ttgtcgcttg tataatgatg gttttacagc gtatattgat ttcagcagtc
     5461 actattccta catatgcaac aaaccctggt cgattctaca tctatacatt tttacaaact
     5521 tccgttgtta ttggtgcaaa tttgatccca ctttacatgg gttaccaata tcaaaaaatc
     5581 gcccgtttaa aagttttaca ttatttaagt cgttttgcct ttatattcct aatagccaca
     5641 ctagtttgca atatcttttt ttacgtacaa gctggcagct tgaatattcg tgattattgg
     5701 cttatcctaa cacctatcag tcagaattac tttacttatg ctgtttcttg tactttgctt
     5761 ttttgtagta ttccttatac agttggattt ttaaataaat tatcgaaaac aacaattaaa
     5821 tatctactat tatttttaac aattggatta gttggctgct ctactctatt taataaagac
     5881 ccgtttgcat tacagaatgg acagagtatt ttatggattt tttatttatt tttaatgggt
     5941 tatggtttga aagaatggaa ctggaaagaa aaggtccgct ataagcttcc tcaagtattt
     6001 atcagcttga ttgttttatt cggtttgatt attttgatga cacaaatttc actcatcatc
     6061 agaggaaata cagatacagc gctgaggttt tctaaaccat attctctctt cgcaatgtat
     6121 tatactttct cttcgttttt atttttagaa atgctatctg ataaattagg tataaaagga
     6181 agaggaaatt ttcttgctgt atttttgatt gtcactcaag ttatatgtaa tttcccttta
     6241 gtttcttatc gcgtatctac atttatgaaa cgtgaatttc caaactcagg gaaggcatgg
     6301 ctgataaata tcgggcagtt ttttatttga tatttattag cagtgtccct cctagttatc
     6361 tttttatttt atatacaaaa aatccgtttc attcatcgtt tcatcgaata cttcgctttt
     6421 gattcgattg aacaattaac tcagcgttgg aaaaaaagaa ttcattggct ttttgttcat
     6481 aaaagttatt ttttagttgc agcatttttt tactgcttta ccttcgtgca gattttcgtt
     6541 ttagaaccaa aagaaggttg gaaagaaact atccaggttg ccttaaaaat atttactcag
     6601 cgacaagcac ccatgatcct gacaatcatc attattatgg gatttttctt cttactattg
     6661 ttgatttcca atcgtttttg gtacgccttg actgctacct tgatcattaa cttactgctc
     6721 actatttcga cagtaatcaa aatggaaatg cgggaagagc ctgtctttcc ttcagattta
     6781 aaaatgctga ccggattgtc agaactatta tcgatggtta gcccagtctt attaatcgtg
     6841 ggcggattga tccttctcct attattgatt actagtatta ttgtacaacg tagactacaa
     6901 aaacagtatt ccttgaagat tcattggaaa cgtcgattta tcagtatcgc tgtattatta
     6961 ggatgttttt cgggtgtctt tttcattaat cacaaaaatt cgccttcttt cttgctgttt
     7021 aatttattta aagtaaacaa gactttcttt aaccagcaag gcgccgtaaa agataacgga
     7081 ccagtcattc aattcctaaa caatatcgac attaaaatca tggaagaacc tgctggctat
     7141 tcgaaagaaa aaattaatgg aataatgaaa aagtacaaca caaaggcaga tgaaatcaac
     7201 caaacaagaa gtgactggtt aaacaatcag acgatcgttc tcaatttaag tgaaagcttc
     7261 agtgatcctg cacgtgtccc taatctgaca gtacctacag accctattcc ttacatcaaa
     7321 agcattataa cagaaggaac aggaggattg atgttatccg tcggttatgg tggcggaaca
     7381 gctaacatgg aatgggaagg actgacaggc ttgagtatca gtaacctgtc tgcttcgtta
     7441 gttacacctt atacccagct agtggagcgt cagaaaataa gtccaaatat taccgatcta
     7501 ttcgatgaaa aaatagccat ccatcctttt acagctgctc tttatcgcag aaaacaagtg
     7561 tttgataaat ttggttttga caggttctat tatttagaca gtccggataa attaacttat
     7621 acggataaaa tagaacaaag cccacgcata tcggatgatt cagcatacaa agaaaccttg
     7681 aaagctttac aaagcaatac ggaaacgtca caattcatcc aattatccac tatgcaaaac
     7741 catatgcctt atgatgatta ctatagtgaa ttgaactaca ctgctgaagg aactgctgtc
     7801 attcctaata gaagaaaaga attacaaact tatatgcaag gccttcacta taccgataca
     7861 gcagtaaaaa aattcatcca agaaatcgac aaaatcgata aaccgattac ctttgttttt
     7921 tatggtgacc atttacccgc aatctatagt gggaatgatt cgaaaaaata tggattagaa
     7981 cagcaccaga cagattattt catttataac aacaagtata gtcgtgaaca agcagctacg
     8041 ttaaataaag aagttgttgc tcctaataac tttggtgcta tggctcttga gcatgcgaat
     8101 atcaaagtaa ctccttacta tgcgttgttg acagaagtat ctaatcatct acctgcatta
     8161 acaattgatc ctacagaaag cttgtctaat cgttttaatg gaaaacaggt ctttgttaca
     8221 gaggatagtc aaattatcca agaaaaaaat ttaactgaga aacaaaaaga aattctagaa
     8281 gattatcgtc tgatacaata tgatcttgtt gcaggtaacc agtacagtgc aacatgggcg
     8341 acacaaaaaa taaaagacta acaaataaac tgagcaaggg gttgtgataa aagacatgtc
     8401 acaacccctt gtttatctca ttaatttgct tgtccataaa cgatttgatt aaagccattt
     8461 ttcaattcat tattcgctgt ctcgttgaag taaagataat tatcgggatt cccctgattt
     8521 tgttgtgtca ccaatacttt tggctgattt tgctgaaacc cgaatagata gacgtgcttt
     8581 ttcagatatg gttctgtctc tgcatcggaa tagacggctg tgatttgata gtctgcttga
     8641 cctgtcccac tttctgacca ttctgcagaa acaggcactc caccaatagc catcttccac
     8701 tccccgttca aaatagcttg agggacttta agaccatata agtccacact catttgattc
     8761 gtataacttt tgtattgttg tcccattgtt tggccccatt tggtcataaa tgcctctaat
     8821 tgtgcggatt tgtctgcatt ccacagtgtt tgttcaactt gttcgctgct ttctgaagag
     8881 gaagatgaag cctctacaga agaacttgaa gtagatgttt gagttgtttc agtcgttttg
     8941 gaagactctg acgaagtaga ggtatctgct gtttctgtac ttgctgcagt tgacttgctc
     9001 tctgtgctag ttgttgtagt agttgcaact gttgctttcg ctgtttcttt tgttgattct
     9061 tttttcgatt cattcccgtt ggtacaacca gctaacaaaa aagctagacc aattgttact
     9121 aaaatccttt ttttcaatta aaaaactcct ttcgattact cccacttctt tattctatca
     9181 aataagcaat gtgattttat tgatttttcg tattttctca tcaatctttc aaatcttcta
     9241 aaaactgaga agcacccgct gatgttgtct gtaatacatc agcgggtgct tcttttattt
     9301 taatcttcgt gattttccaa tttacctcac gctttatatt tgtatttgaa gaaacataga
     9361 cttttagtca tcatcgtctt cctcatccaa atcttgtcgg acagtttttc cttggagtct
     9421 ttgtacttgg tagatgattt ctttattgaa acttaaaagt tcaacaagca attgttgcaa
     9481 gccatatttt tcttcttttt cagctaattt gattgcccgt gtgataaaga cattttggaa
     9541 atcaaaatct tttgcaaagt tttccaacat tgttgaggct ggtaaatatt tgttttgacc
     9601 atgttcgtaa atttttccat aacgcatgac ttcttctaaa gtagacggaa cgatttcatt
     9661 ttcaatcact aactcatgag agattttatt gaaccatctc gcttcttgat cagcatattc
     9721 tgaaaaaagg gtacttaatt cttgcgaaga tgtacccaaa acatacaatt gtgcttggat
     9781 caatttcatt tcttctaatt taaaatttgc taatacatgc cccaccatgg cgccagcagt
     9841 tggtttatga tggtcgatat ctgcttgttt tgtttcttga acaaattttt cttccactat
     9901 attcattgat ttacgcctct tttacttttg ctgattggac ttcataacct aatttttcca
     9961 caactgcagc taattgttca gggtggactt catctggatc aaagtttgct ttgattttgc
    10021 tcgcattaaa caaaactttc gtattgctta ccccaggctg ttgagaaacc gcatgttcga
    10081 ttttttgcat acagcttgga caagctagtg tatctaattg cataataatt tttttcatcg
    10141 tagttcttcc ttcctttttc ttgatagtct tattctagat tacagcttcc ataaaaaaat
    10201 tgattcttat caagttttac gatttttctt ctttgctttc gtgtccaaaa tacaataaac
    10261 gcatcgcgtt aaagataacg atcaagatac tagcttcgtg aacgaacatc ccactagcca
    10321 tgtaaataaa cccggcaaat aagccgttga gtaaaaataa gacgacacca atggcaatca
    10381 ctacattttc acgggtattt aaaacagttt tcttagccaa gcggtaggca tgaaccaacg
    10441 attcaaaatt agattgcatc aagacaacgt cactcgtttc gatagcaaca tccgttcctg
    10501 atcccatagc aatcccgata tcagccgcag ccaaagaggg agaatcattg attccatctc
    10561 caataaaagc aacgcgcaat ccttgttctt ggtatttttt cacaaattga accttttcgt
    10621 ctggtaataa ttcagcatgg acttcatcaa tgcctaacat atcagctaca taatcagcag
    10681 tcatctgatt atcacctgtc agcatcacta actgttttgc tcctgcttgt ttcaacttag
    10741 ctaattgttc agcgacttct ggtcgaatca catctgacac acctatgatt tgagtgattt
    10801 ttttgtcgat tgctacaata atgaccgagc tgcctgtttg ttgtaattga tgtaaatctt
    10861 gtacttgctt tggtgtgaga gaaagtttgt gagcggcaat caatttggtg ttgcccgcac
    10921 aaactgtatg accatcaatc tcagctgtga ttccttgtcc cttgattgtc tgattatctt
    10981 tgactgctaa ttgttgaaaa tcaatgtctt tctcctgagc ataatggatc acggcgcgcc
    11041 ccagaggatg atcggataac gtttctaatc gagccactaa agctaagctg tccccttcat
    11101 cctctgtata gttcttgata gtggatacag cagtagttcc tttggtcaat gtaccagttt
    11161 tatcaaagac aaaagtatcg attttggaaa aagtatccat tgcttcaccg cctttgacta
    11221 agatgccccg tttcgctcca ttaccgattc ctgctacatt cgaaacaggc gcaccaatga
    11281 ctaaagcgcc aggacaacca agaaccaaaa cagtgattgc taaacgaaaa tcacgactaa
    11341 tcagaccaac aaccaacgca ataagtaaaa cggttggagt ataatactga gaaaatcgat
    11401 caatgaattt ttccgcatgg gatttggtat cttgtgcttc ttctactaat tcgataattt
    11461 tggcaaaggt cgtatcatct ccgaccttag ttgcttcaat cgtcacgtag ccatctgcta
    11521 aaatcgttcc agagaagact tcatctccac tgattttgac gacttgtcgt gattcaccag
    11581 tcactgcggc ttcatccgca taccctttcc cgtcaatgac tcgtccgtcc acaggaatag
    11641 ctccaccagt ttttactaag acgatatcgc cttcttccac gtcatcgatt tccacttctt
    11701 cagtcgttcc atcttcttgg acaacactag cagtagatgg ggccatctcg gtcaaagcct
    11761 tgattgactc ccgcgttttg gtcaatgttt tttgttccag atagctacca aacaaaaaca
    11821 agaatgtaac gatagctgac tccttgtatt ctccgatcat aaaggcacca agcacagcaa
    11881 tagtaacgag taaatcaatt gagataactt tgactttcac tgcctgatac gcatggatca
    11941 taataggtat gaccgcaata atagaagcta tggctagagc aatgttatag aacaagacaa
    12001 aaccagtaaa ttttccgata aaaccaaaaa taatcaacac agctgataag gcggtgatat
    12061 gatttcgatg ctcttggatc cacttttgta atttcatctt tctccctctt ttcctttcat
    12121 tcttacgaga ctaagtatag atacttatcc atcataaaaa attgaccact atcaagttta
    12181 gtcaaaaagg cggttttctt ttggtagaat acactacaaa aaatagttag caagaagact
    12241 cactaaaagt gatcttgcca actattgtcg tttctatcga aattttcatt gaatgatttt
    12301 cctgtacagc atacatcgga gatctatttc tttatctaag atttttatct tgctaatttt
    12361 ttcctttagt ctttcaaatc cacgtcgttc atagaattga tacgtacagc cactatctgt
    12421 aaaaaggaat atttcttttc ctttctctct tttttcaaat tctgttagca acttggtacc
    12481 gatgcctttt gttttcaaat caggatttgc agctaaaaaa atgatctctc catctggcga
    12541 atgcttcttt aaatatgctg caagcatttg ttcatttgct gtctcgtaag ctcctttact
    12601 ttctttagca aatatatttt gtaaaaaatc gaatatctta acatatattt gctgtccaaa
    12661 agaacgatgt ttcttttttt ctccttttat ctccgccagc aaaacacctg ccaactcgtc
    12721 ctctgcatac aaagcaagaa tttgagttgc tcgattcaac tccatataca aaaaatatct
    12781 tccgtaaaga ttcagcagaa gttcattttc catatatctc tcaaagttca ttccttcaac
    12841 agcaaaatag atagcttttt tgtaatcttt cttttctaag ggtttaaaag ttagtttcat
    12901 catttcgcct cctcaacttc aatgattgtg ttatacttac attataatag acacagtgtc
    12961 tactatctat aatcaatcat ctataagttt gtcgattaag cgacagcttt gctttgatat
    13021 gtcggataag gagataggta aatgaaaaag acagatagac gcgtcaaaaa aacagaaaaa
    13081 gctttggctg aaacactatc cacattatta gtaaataaaa agattcaagc aatcactata
    13141 cgcgaactaa cagaaacggc agatgtacat cgttcgactt tttatacaca ttataaggat
    13201 atctatgatc tatatgatca attagaaagc aacttttttc gggatctaaa tgaaattctc
    13261 tcttatgatc cagcccatag ctatgaggaa ctatacactc gcttgattga ctatgtttac
    13321 gcaaatactt ttttatatcg cttatttatc ctcaaccatg gtgatatttc gtttcagaag
    13381 aaagttaaac aagttattga agaaaactat ctgaacatat ggctatatga agacaagaaa
    13441 acgattatca cagaagaaat gagattcttt actacgtacc acgtccaagg ttgtctatcc
    13501 gttctcaatc ggtgggtaga aagcaattac aagtactcca aaaaagaatt attagaactc
    13561 ttacagaaac tgaatgatca catagagaaa atcatgcctt gaataaaaac atactaagct
    13621 tagttaagaa gaaccgtaac cttctcgtat aacaactgaa tcttatttag ccaaattgtt
    13681 tactgaagat tttttgagta aaatttctat aattcctact tattgaaaag tagtttgtag
    13741 tgctgcttct ccctctctaa gtgatatatc ttgttgtacc aatcaaattc acctcatcat
    13801 taataaatta cttttaggct aatttattac aacttatagg ttgaagctgt aaccaattta
    13861 tagtcatatt atatttttat catccagttt attgaattct tctcgtttac ctcaattcat
    13921 tttcaagggg aatca
//
LOCUS       DAZAID010000071        13879 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000071 DAZAID010000000
VERSION     DAZAID010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.071"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..2319
                     /gene="acm"
                     /locus_tag="ACG9BE_002055"
     CDS             154..2319
                     /gene="acm"
                     /locus_tag="ACG9BE_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFN3032791.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2591..3076)
                     /locus_tag="ACG9BE_002056"
     CDS             complement(2591..3076)
                     /locus_tag="ACG9BE_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFN3032792.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3166..3511
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3556..4197
                     /locus_tag="ACG9BE_002057"
     CDS             3556..4197
                     /locus_tag="ACG9BE_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN3032793.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4216..4851
                     /locus_tag="ACG9BE_002058"
     CDS             4216..4851
                     /locus_tag="ACG9BE_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN3032794.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4869..5684
                     /locus_tag="ACG9BE_002059"
     CDS             4869..5684
                     /locus_tag="ACG9BE_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3032795.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6003..7439
                     /locus_tag="ACG9BE_002060"
     CDS             6003..7439
                     /locus_tag="ACG9BE_002060"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3032796.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7628..10060
                     /locus_tag="ACG9BE_002061"
     CDS             7628..10060
                     /locus_tag="ACG9BE_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFN3032797.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            10290..11357
                     /gene="rlmN"
                     /locus_tag="ACG9BE_002062"
     CDS             10290..11357
                     /gene="rlmN"
                     /locus_tag="ACG9BE_002062"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFN3032798.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRSEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11347..11553
                     /locus_tag="ACG9BE_002063"
     CDS             11347..11553
                     /locus_tag="ACG9BE_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032799.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11550..12653)
                     /locus_tag="ACG9BE_002064"
     CDS             complement(11550..12653)
                     /locus_tag="ACG9BE_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN3032800.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13205..13753
                     /gene="aac(6')"
                     /locus_tag="ACG9BE_002065"
     CDS             13205..13753
                     /gene="aac(6')"
                     /locus_tag="ACG9BE_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN3032801.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4752 a         2298 c         2937 g         3892 t
ORIGIN      
        1 tttggcatat ttatttttta taaatatttg taaaataact atcaattttt tagttaaaag
       61 attggaaatg agtgggggag gaattgaaga aaggattttt gagagatgat atagtaggtt
      121 ttatttttag ttaaaatagg ggagatagaa aaaatgaaaa agtgtagaaa tattatttta
      181 tcaatgttat ttattattac taatataaca tcactgattc ctgttcatgt ctatgcggat
      241 gcaggcagag atatcagcag taatgtcact tcgttgaccg tggacccaac gaatattaca
      301 gatggaggaa acataaaagt aaaattctcg tttgacgaaa aaaaacaaaa tatccaacca
      361 ggtgattatc tttggatcaa ttggccaagt gaaggaaata ttcgcggtga aggattccaa
      421 aaagaaatcc cattaatgat tgaaaataaa aatgttggaa ctttaacggt cagaaaagat
      481 tctgcgcagg ttgtgtttaa tgaaaatatt aaaaacctag actcagtaga aggttggggg
      541 caatttgaga tacaggccag aaacgtaacc gataccggtg aagaaaatac gggaccattc
      601 accgtgacga gcggtgataa aacagctact gtaaatgtga caaagccagc ttctggttct
      661 tcttctagcg ttttttatta taaaacagga gacatgctac ctgaagatac taagcacatt
      721 cgatggtttt tgaatattaa caataacggc acttatgttg aacaaccagt taaaatcagt
      781 gatgaaatcc aaagtggaca aagattagat ccaagtacgt ttgaaattaa tcaaatacac
      841 ttaggtgaac agaaggttta tagaggagaa gagggaatcc aacaattttt acaagatttc
      901 cccagcgcta catttaattt ttcagtaact gataattaca ttgaaataac tataccaaaa
      961 aactttgtca atcttaggaa aataatggtg agttataaga cgatcataga aaacccagaa
     1021 caaataaatt ttgagaatca ttcggaagct tggtttaaag aatttaataa acctgctgtt
     1081 gatggagaaa gtttcaatca tacagttaaa aatatttcag cttctggtgg agtcaacggt
     1141 acagttagag gcgaattgaa gattttcaaa tatatcaatg acacagagat tggtatccca
     1201 aatgtgactt tcgaattaag acgagcagat gaacagccta ttcaaggaca gtctagtatc
     1261 ctgttgactt cgaatgaaca gggagaagct agtatcaaag gcctgcaagt aggagactat
     1321 gttgtaaaag aaaaagaagc acccaattgg attgattttg atccactaag ttcaaatgaa
     1381 ctcaaattct ccataaacga aaatgataca gagggagtca gtttacctat ttataacaag
     1441 aaaaaggtta cgaacatcac agcaactaaa atttggaacg gtggaaccac tccaagaccg
     1501 agtatttatt ttaaattatt cagagcaagt acaaacaatt gggaaccagt tcctgatgca
     1561 gaaacaaaac gattagacaa tggaataact tcagtgactt gggaagatat ccaacagtat
     1621 gatgatagtg gaaacgaata tacgtttaaa gtgcaagaag tagatcaaaa cggaaatgat
     1681 tatgtccctt caggttatca aaaaattgaa aacgggctag tcgttacaaa tgagaataaa
     1741 gaagtcattt cagtctcagg acaaaaaaca tgggaagaca atgagaacca agacggcaaa
     1801 cgaccgacga cgatcacagt aaatctatta gccgacggtc aaccgatcca acacaaagaa
     1861 gtaagtgaaa aagatgattg gcgctatcgt ttcacaaatc tgccaaaata cagagacggg
     1921 caagaaatca tctatactgt aacggaagac aacgtgcccg aatacagtac gacgattgac
     1981 gggtataaca tcaaaaacag ttatacaccg gaaaaaacaa atatatctat accaggtagc
     2041 catataacac cgtggaaaaa ttttccagag aagacagaga gagagaaaat tagaaatcta
     2101 ttctctcaaa aattgcaaat atttgctcat acaggagaat ccagcgaaca aagaagcaat
     2161 tatattagta agagagctgt acctaaacag ataacaaata aatcaaaaag cattcttcct
     2221 aaaacaagta gtaaaaaatc tagttttgct gtaatcattg gtttattgat agtaactatc
     2281 tgtgctggga tattcttaca aaaatataaa aaagtttagt tagaatataa tattttttgt
     2341 taaatatgac tgaaatcaaa agaataaaat tccgatttat aatcgaaaaa agaagcattt
     2401 ttatatatgc ttcttttttc gtagaatcaa taaatatgga tttggctaac tatggaagaa
     2461 tactcgtatt agttttcagc gttcatacta gttgttacat tgtttgattg gccaaaagaa
     2521 tttctgatat attttgtgct aaataaagaa aaagacgctt gaattgggaa ataagaaaac
     2581 tactctatca ttacaaattt tcataaactg gcagatgttc taagtaaaca agtatctttt
     2641 caaagtcgga aaaatccata atcaacgtag tggtattgat atttggatga aagcctacgg
     2701 tatcttgacg atcaatagca gcatcgatca cgacttggat ttttttctct ttgtcgtgca
     2761 tcaatgctaa tggagtcact gtacctgctg gaacgcctag gaggtcagga agctgttctt
     2821 cagaaacaaa agataagcgc ttttctttta attgttcagc tagttgtttt aaatcggcgt
     2881 gcttttcatc aggcaagatg acaagataga tttgttttcc ttttcttgat ttcaataata
     2941 gattttttac ttgcggacca ggtagggaaa aagggacatt tttgactgac gtaatcgcag
     3001 gatgatcgac tttttgaaaa gagataccca gtttttttag tagttcataa gcttcttgtt
     3061 cggttgcacg actcatcgcg catcaccctc ctttttttat tagtgtatca taaaaaagag
     3121 ttgacaaatg aaaaataatc atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa
     3181 agaagagtag ccgttaggtg tttttagaga gtctctggtt ggtggaaaga ggcagcagtt
     3241 aaccgtgaaa cacatcttgg agtcgatttt ctgaagcgta tagtaggaaa atccggggag
     3301 cccgttacag ctgcggtcgt tgctagatga ccagataagg ctggcttttc cgtgaggagt
     3361 cggtaaaaaa aggtggaacc acgtgattaa cgtcctttga tcttcggatc aaaggacttt
     3421 ttgttttcat ctgaacggca acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca
     3481 gaggtggaac cacgtataat acgtcctctt ggcatttgcc aagaggattt ttttatttta
     3541 aaggaggaaa agaagatgga gtatttgatt gaagttatgc cgcaattatt agtaggagca
     3601 ctcacaacat tgaaattatt tttatttacc ttgattggct cattaccatt agggattgtt
     3661 ttagcattag gattgtcttt gcattttaag ccgctacgct acttgcttca aacatatgta
     3721 tgggttatgc ggggaacacc gctgttgttg caactgattt ttatttttta tgggttgccg
     3781 acgattggga tcgtttttga tcgtttcgaa gcagcactca ttgcctttat cttaaactat
     3841 gcagcttatt ttgcagagat ctttcgtgga gggatacaat cgattcctca aggtcaatat
     3901 gaagcagcgc aagttttgca attaagtccc tttcaaacgg tgacgaaaat cgtcatccca
     3961 caagtgacaa agattgtttt accttctgta gggaatgaag ttatcaactt ggtcaaagat
     4021 tcttccttga tctatatatt aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg
     4081 gaacgcgacg cgactttact gcctttgcta ggcgtggcaa tcatctatct tacattgact
     4141 ggtattctaa caatcgtaat gaaacaagtt gaaggaaaat taaattatta ccgctaactg
     4201 ataggaggaa gaaatatgat tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa
     4261 ataatcaaag atttgaatct aatcattcca gatgggaaaa cattagctat cgtaggtcct
     4321 tccggtggcg gtaaaaccac gcttttacgt atcttggctg gcttagaaag ttctgacagc
     4381 ggagaatttt atttagacgg tgtaccgttc aacccattcg agcttaaaat gaaagaacaa
     4441 gttgtaggag tcgtattcca agacttccag ctatttccac atctgaatat ttttgaaaac
     4501 attaccttag caccaaaaat ggtattgaaa cagacaaaag aagaatacac caaaaaagca
     4561 gaagaattag cggcttcatt agggattagt gatttattga atatctatcc ttatcagctt
     4621 tctggcggac aaaaacagcg ggtagcgatt gccagagcat tggctatgaa tccacgggta
     4681 ttggcttatg atgaaccaac aagtgcttta gatcctgaat tacgccagca agtcgagcgt
     4741 ttgatcctat cattgaaaga agatggggta acccaaatta ttgtcaccca tgatttagta
     4801 tttgcagaaa atgtcggtga tcaattacta aaagtttccc caattcatta agaaaggaga
     4861 agatagagat gaaaaaaacc agttttctat tcgctttgat cgcaggtctt cttctttttg
     4921 caggctgttc gaacaaaaaa acttcagcag ataattgggc gactatcaac gaagagaaaa
     4981 aaatcgttat aggtttagac gatacatttg ttcctatggg atttagagat aaagacggga
     5041 agctcacagg atttgatatt gatttagcca aagcagtctt tgaacaatat gggattacgg
     5101 tagactttca gcccattgac tggtcaatga aggaatttga actgaacaat ggaacgatcg
     5161 atttgatatg gaacggctat tccaaaacgc cggcaagaga aaagaaagtg caatttacaa
     5221 aaccttatat ggaaaacgat caagtgctga tcacacctaa agaatcagga atcacaaact
     5281 ttggacaaat gaataataag agattaggtg cgcaaaatgg gtcatctggt tatgatgtat
     5341 ttacgaaaca accagaagta ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg
     5401 atagttttaa tgaagcattg atcgatctga aaagcagacg ggtcgatggt ctgctgatgg
     5461 ataaagtata cgcagactat tatttgaaac aaagaaacga actagaagac ttcaacataa
     5521 caaaaggttc ttatgagagt gaggattttg cagttggtgc acgcaagtca gatagtacat
     5581 tagtgaaaaa aatcaatgaa gccctagatc aactagagaa aacaggtgaa ttccaaaaaa
     5641 tatccgataa atggtttggc gaagatgtta cgccgagaaa ttaacaaata ggggactgtg
     5701 acaaaagacg tgtcacagtc cctttttccg aataaacagt gttctcctgc caactccttg
     5761 tgactcggag ctgaatggca ggctcacaac ctcttggtaa acggtgttca aaagccagtt
     5821 tttcggtatt aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct
     5881 tactactcga aactaagcga ctttttcaca acctccttta cataatttta ttttgttgtg
     5941 gaaggggttt cgtgttattc taaaagagga acgattgaag gttataggaa aggaagagat
     6001 gaatggaaca caaagaactt acctcatttc caaaagattt tctgtggggc tctgcttctg
     6061 cagcgtatca agtagaagga gcttggcaag aagatgggaa aggagaatca gtctgggatc
     6121 gatttgtacg gatacctgga aagacattta aaggaacgaa cggtgatttg gcagtagacc
     6181 actatcatcg ttataaagaa gatattgctt taatgaaaga acaaggtctg aaagcttacc
     6241 gcttctctgt agcttggaca cggatttttc ctaatggacg aggagaagtc aatcaagctg
     6301 ggctcgcttt ttatgagaga ttgatcgacg aattgattga aaacgatatt gaacctatgt
     6361 tgactttata tcattgggat cttccacaag cattgcaaga tgaatataat ggttgggaat
     6421 cacgtcagat cattgaagat ttcactcatt atgcagaaac attatttgag gcattccgcg
     6481 ggaaagtcca ttactggatt agtttgaatg aacaaaatat cttcacttca ttaggttatt
     6541 tgttagcagc acatccgcca ggtgtgacag acccgaaacg aatgtatgaa gttaaccata
     6601 ttgctaattt agcaaatgca tctgtcatca ataaatttca cgagttagaa atgcctggaa
     6661 aaatcggacc aagctttgcg tatacgccaa attatccaat tgacagtaat cctgaaaatg
     6721 ttctggctgc tgaaaatgca gaagacctga tggccaatta ttggatggat gtctatatgt
     6781 ggggaaaata tccgattgct gccatgaggt tcttagaaga aaaaggatgg gcaccaacga
     6841 ttgaagctgg cgatgcagaa ttgctagaat ctgctaaacc agattttctt gggatcaatt
     6901 actatcaaac ggcgacgaat gcattcaatc cattaaacgg agttggtgct ggaaaaatga
     6961 atacgacagg aaaaaaaagt tcatcagaag aaactggagt tcctggaatg tataaaaaag
     7021 ttgagaatcc atttgtcgaa cgaacaaatt gggactggga gattgaccca gaaggattaa
     7081 ggatcggact gcgtcgtatc acaagccgtt accgcatgcc ggtactgatt acggaaaatg
     7141 gcttaggaga gtatgacaag ttgacagaag ataaacaaat ccatgacgac tatcgaatca
     7201 attacttaca aagccacgtc aaagccatca aagaagcaat ttcggatgga gcagaagtcc
     7261 taggctattg cacctggtct tatacagatt tgcttagctg gctgaacggt tatcaaaagc
     7321 gatatggatt tgtatatgtt gatcaagacg aaacacaaga aggatctttg gaacgatata
     7381 agaaagacag ttattactgg taccaaaaac tgattgaaga aaatggagag aacctttagt
     7441 tcacatactt aaaacaagaa ggtacggctg gatatggccg tgccttttct tgataaaatc
     7501 atttagaaat taagaaaaac tgtaaaataa gctgaaaaat gaaagaaagc gtcagttctt
     7561 tcaaacaaaa ctttagttcc cttacttttt tttgtataat gaaacttgat gaaatgaggg
     7621 ataataaatg gcacaaaaga aacggcctac taaaaaaagg aaaaagacga aaaaaacgaa
     7681 aaagcaaaaa cagcagcagg aacaaatgct gacgattggt gttgggtttg ccttgatttt
     7741 atttgctgtc ttcggctttt taaaattagg ttttttagga atattgattg caaacggctt
     7801 tcggatcatt gcaggaaata cttaccaaat cctctgtctt ttattagccg tattaggatt
     7861 ttggattgtc attaagaata cagagttttc aattggtaaa aaccgacgct ggtttggcgg
     7921 catactcttt tatttcggta tcttattatt gttacatgcc catttatttg gcaaactcca
     7981 tactggggaa ccaaatatca tggggacgac atgggatttg ttagccagtg atataaaaca
     8041 aagtcaagta gacaataacg ttggtggtgg gatgattgga gcaatccttt atcattttac
     8101 ttacttcttg attgcacagc ctggaagtta tttagcagca gtgctattac tggcaggagg
     8161 agcattcctg ttcagtaatc tggaaggcta tcagctgtta aatggtatcc aatcaattgg
     8221 agaacgagta caggaattat tggaaggtga tcctgccaag caagctcgaa agcaagcggc
     8281 aaaggaagag agaatgaaac agcgcgcaga agcgaaagaa gcaaggcgct tagctgcaaa
     8341 agaagctgca gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga
     8401 tgaatggcag gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag
     8461 ttttcaagaa aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca
     8521 gaccaataca gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga
     8581 atttgagatt gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct
     8641 tgattctata ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg
     8701 cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt
     8761 aggaccatcc gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat
     8821 tgtgaactta acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc
     8881 accgattcca ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt
     8941 ttcctttaga gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc
     9001 tttaggacgt gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt
     9061 actcgtggct ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag
     9121 tatattaatg cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt
     9181 tgaattgaat atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag
     9241 aaaagcagcg caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt
     9301 cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt
     9361 ggaaaacgga acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc
     9421 tgatttgatg atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat
     9481 ggctcgggca gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat
     9541 cacaggaatc atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac
     9601 agattctcgg acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat
     9661 gctattcttg ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga
     9721 ccacgaagta gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga
     9781 gaaaatgatg ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga
     9841 gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct
     9901 ccaaagaaga tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc
     9961 tcatggagtt gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga
    10021 agaaacatca gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt
    10081 gaaaaacgaa taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt
    10141 tttccatctt ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt
    10201 tctattcgat ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc
    10261 gaaactttga gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga
    10321 atgaagaatt aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag
    10381 tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga
    10441 aacaattgat cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg
    10501 tccaagaagc ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga
    10561 ttgagactgt actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag
    10621 gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga
    10681 ctgctggaga aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc
    10741 ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg
    10801 acaatgtaat ggactttttg catatcatca atgatgccaa aggattggca attggtgctc
    10861 ggcacattac agtttccact agtggattag cacacaaaat caaggaattc gctaataacg
    10921 gcttgcaagt caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga
    10981 tcatgcggat caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt
    11041 tagagaaaac aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata
    11101 gatcagagca tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg
    11161 tcaacttgat tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag
    11221 ctgatgtatt gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta
    11281 aagaacatgg tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa
    11341 aaacagttgc caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt
    11401 ttcttaatta aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata
    11461 aagctttttt tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca
    11521 gcctgcttga tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt
    11581 tcttgtgtag catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg
    11641 atttcataag gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct
    11701 aaatcggtcg tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc
    11761 ataaacacat agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt
    11821 acaccataac tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta
    11881 ccgtaataga atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc
    11941 aatccggtag taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg
    12001 tcggcactat ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt
    12061 ccgatttgga ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta
    12121 gattcgactt ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct
    12181 tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt
    12241 ccgtccatta cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc
    12301 gaaccaatag tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca
    12361 gtatcgcaag cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca
    12421 tgctggatca actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg
    12481 acaacctcta tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca
    12541 gtcttccctc caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt
    12601 tcatacgcat ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt
    12661 tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct
    12721 gtagatggag tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac
    12781 cttgaaatca tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa
    12841 taatgaattt ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc
    12901 taaaaattct aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat
    12961 tggttttttt taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc
    13021 caattgatgg ctttcctgat ctttcctatt ttgaataaag tgtttctgta taatttttta
    13081 ttttataagt aagataaata atgaaagaat agagatactg attggtagct aaagattttt
    13141 gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac aagggggaag
    13201 acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc agctttctga
    13261 tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag aagtagaaga
    13321 aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt tagtaggatt
    13381 tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat tagttgtaga
    13441 aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag aaaaagaagt
    13501 agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc atggaacaac
    13561 gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca tccagaacct
    13621 tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag gtgtcttacc
    13681 aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta ttcctcgacc
    13741 aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga aataagaagg
    13801 aatttacgaa aattacttct tgaatttttg tgaaattcca acttatttcc gaagaagcta
    13861 cttttggaac accgtgtac
//
LOCUS       DAZAID010000072        13708 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000072 DAZAID010000000
VERSION     DAZAID010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.072"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..350
                     /gene="yaaA"
                     /locus_tag="ACG9BE_002066"
     CDS             108..350
                     /gene="yaaA"
                     /locus_tag="ACG9BE_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFN3032802.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            337..1461
                     /gene="recF"
                     /locus_tag="ACG9BE_002067"
     CDS             337..1461
                     /gene="recF"
                     /locus_tag="ACG9BE_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFN3032803.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1458..3404
                     /gene="gyrB"
                     /locus_tag="ACG9BE_002068"
     CDS             1458..3404
                     /gene="gyrB"
                     /locus_tag="ACG9BE_002068"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFN3032804.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3424..5895
                     /gene="gyrA"
                     /locus_tag="ACG9BE_002069"
     CDS             3424..5895
                     /gene="gyrA"
                     /locus_tag="ACG9BE_002069"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFN3032805.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6131..6430
                     /gene="rpsF"
                     /locus_tag="ACG9BE_002070"
     CDS             6131..6430
                     /gene="rpsF"
                     /locus_tag="ACG9BE_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFN3032806.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6480..6992
                     /gene="ssb"
                     /locus_tag="ACG9BE_002071"
     CDS             6480..6992
                     /gene="ssb"
                     /locus_tag="ACG9BE_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN3032807.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            7018..7254
                     /gene="rpsR"
                     /locus_tag="ACG9BE_002072"
     CDS             7018..7254
                     /gene="rpsR"
                     /locus_tag="ACG9BE_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFN3032808.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7397..9370
                     /locus_tag="ACG9BE_002073"
     CDS             7397..9370
                     /locus_tag="ACG9BE_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFN3032809.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9376..9828
                     /gene="rplI"
                     /locus_tag="ACG9BE_002074"
     CDS             9376..9828
                     /gene="rplI"
                     /locus_tag="ACG9BE_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFN3032810.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10102..11469
                     /gene="dnaB"
                     /locus_tag="ACG9BE_002075"
     CDS             10102..11469
                     /gene="dnaB"
                     /locus_tag="ACG9BE_002075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFN3032811.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12249..13541
                     /locus_tag="ACG9BE_002076"
     CDS             12249..13541
                     /locus_tag="ACG9BE_002076"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFN3032812.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4575 a         2471 c         3016 g         3646 t
ORIGIN      
        1 ttagattaaa tcgtcttata tggaaaaata tatggataaa tttgttttat tgcgaaaaaa
       61 aaggtataat agagtatact tttaataatg aaaatgaggg atgggcattg aaacaacaag
      121 tcattctaaa tacggaattc atgacgttgg gccaaatgtt gaaagaagtc acagttatcg
      181 gtagtggcgg acaagcaaaa tggtatttgg cagaaaacac tgtgttagtc gatggcgaac
      241 cagaaaatcg tcgtggacgc aaactttatc ccggcatgat ggttgaagtt ccagaagtag
      301 gaactttttt tatggtaaaa aaaggaaacg acgataatga ggctgaataa gctgtattta
      361 aaaaattatc gaaattacga agagctgaac atcgaatttt ccaagaacct cgtcatcttt
      421 ttaggagaaa atgcgcaagg aaaaacgaat cttttggaaa gtatctacgt attagctatg
      481 acgcgaagtc atcggacaag tagtgagcag gaattgattg gctggactga cgatcaggcg
      541 atgattcaag gagagatcac aaaaggttca tccactcttc cattagaaat cttattatct
      601 aaaaaaggcc gaaaaaccaa gatcaaccat atcgaacaga aaaagttaag tacttacgtg
      661 ggacaaatga atgtcatttt atttgcacca gaagatcttt ctttagtaaa aggcagcccg
      721 cagatcaggc ggaagttttt agatatggaa ttaggccaga tcaatccaat ctatctttat
      781 gatcttgtcc aataccaagc aattttgaaa caaagaaacc agtacttgaa gcaattgaat
      841 gaaaaaaaac aaactgattt gctttatttg gatattttgt ccgaacaact agcagcgttt
      901 ggcagcaaag tattgaaagc aagagcacag ttcgtcaagc gattggagtt ttgggctaat
      961 tctttgcatc agcaaatcac ccatcaaaaa gagcagctgg aaatcgagta tctcaccgca
     1021 gtcgattctt tagaaacaca tacgcaagag caaatccaag aacaatttct cgcacttttg
     1081 aaccaaaata aaaagaaaga cctttttcgg ggaacaacga cagttggtcc tcatcgtgac
     1141 gatctttcct tttttattaa tcagaaaaat gtgcagactt atggatcgca aggacagcaa
     1201 agaacaactg cactaagtgt caaattagca gaaatcgatt tgattaaaga agagacaggg
     1261 gaatatccaa tccttttgtt agatgatgtg atgagcgagc tggatgataa tcgtcaactc
     1321 catctgctag aaacaatcga aggaaaagtt caaacgttct taacaacaac gacacttgac
     1381 catgtaaaaa ataaaatgac tgttgaacct gagatttttt atgttcaaca agggaaacta
     1441 aaaggagaag aaactgaatg acagaagaaa gaagtttagt agaacgcgct aaagagtatg
     1501 atgccagtca gattcaagta cttgaaggac ttgaagccgt tcgtaaacga cctggcatgt
     1561 atatcggttc gactagttct gagggtctgc atcaccttgt ttgggaaatt gtcgataatt
     1621 cgatcgatga agtattagca ggttttgcaa cgaaaatcca tgtgattatt gaaaaagaca
     1681 acagtatcac cgttattgac gacggtcggg ggatacctgt agatatccag gcaaaaactg
     1741 gacgtcctgc agttgaaacg gtctttactg ttttacatgc tggaggaaaa ttcggcggtg
     1801 gtgggtacaa agtttctggt ggacttcacg gagtaggatc ttctgttgta aacgcgcttt
     1861 ctacacagct tgatgtcaaa gtatacaaag aaggaaatgt gtactatcaa gaatatcgtc
     1921 gtggagctgt agtggacgac ttaaagatta tcgaacaaac ggatcgccat ggaacaacgg
     1981 ttcactttac gccagaccct gaaattttta cagagacaac tgagtttgat ttttcaaaat
     2041 tggctacccg gatccgtgaa cttgctttct tgaaccgcgg gatgcgtata tcgattgaag
     2101 acaaacgaga agaagaacca gtcatcaatg aataccatta tgacggcggg atcaaaagtt
     2161 atgtcgaata cttgaatgca aacaaaaccg tgatttttcc agaacctgta tatttggaag
     2221 gtgaacagca agatatcgca gttgaagtct ctatgcaata tacagacggc taccattcca
     2281 atatcatgag ttttgccaac aatatccata cgtatgaagg tggaacacat gaatcaggat
     2341 tcaagactgc tttaactcgt gtgataaatg attatgcccg aaaacaaaag ctgatgaaag
     2401 aaaatgacga caacttgacc ggtgaagacg tgcgagaagg attgacagct gttatttcta
     2461 tcaagcatcc tgaccctcaa ttcgaaggac aaacaaaaac aaaattgggc aattccgaag
     2521 tacggacagt cactgatcgg ttatttagcg aacatttcat gaaattcttg ctggaaaacc
     2581 caagtgtggg ccggcaaatt gttgaaaaag gattactagc ttccagagct cgtctagcag
     2641 cgaaacgtgc gcgtgaagta acgagacgta aaggtgcttt agagattagt aatctgccag
     2701 gaaaattagc ggactgctct agcaacgatc ctgaaaaatg cgaattgttc atcgtcgaag
     2761 gagattcggc cggcggatca gctaagcaag gtcgtaatcg agagtttcaa gccatcttgc
     2821 ctattcgtgg gaaaatcttg aatgttgaaa aagcaagcat ggacaagatc ttggcaaatg
     2881 aagagatccg gtctttattt actgctatgg gtacagggtt tggagaagat tttgatgtat
     2941 cgaaagctcg ctaccacaaa ttagttatca tgactgatgc cgatgtcgac ggcgcgcata
     3001 tccgtacatt gctgctgact cttttttatc gttacatgcg tcctattgtt gaagctggat
     3061 acgtctatat tgctcagcca cctttatacg gggtaaaaca agggaagaat attacgtatg
     3121 tacaaccagg aaaaaatgca gaagaagagt tgaaacaagt cgttgcttca cttcctgcct
     3181 caccaaaacc aagcgttcag cgttataaag gacttggaga aatggatgat catcaactat
     3241 gggaaacaac tatggaccca agcaatcgga tgatggcccg cgtcagtgtg gacgatgcta
     3301 ttgcagcaga tcagatattt gaaatgttga tgggggatcg agtagaaccc cgtcgtgcat
     3361 tcatcgagga aaatgcacac tatgtgaaga acttagatat ttaatcatga aggaggaaaa
     3421 agaatgagtg aagaaatcag agaaaacatc catgatgtca atctaaccag cgaaatgaaa
     3481 gactccttca tcgattatgc gatgagtgtt attgtcgcac gtgcacttcc tgatgtcaga
     3541 gatggtctga aacccgttca tcgccgtatt ttatacggca tgaacgaatt aggtgtaaca
     3601 ccggataaag cccataaaaa atctgccaga atcgtcggag atgtcatggg taaataccat
     3661 cctcatggag acattgcgat ctatgagtca atggtacgga tggcacagcc attcagctac
     3721 cgttatatgc tagtggatgg tcatggaaac tttggttctg ttgacggtga cggtgccgcc
     3781 gctatgcgtt ataccgaagc acggatgagc aaaatcgcta cagaaatgct gcgtgatatc
     3841 aataagaaca ctgttgattt ccaaagcaac tatgatgata cagaaaaaga accagttgta
     3901 ctacctgctc gcttcccgaa tcttttagtg aacggaacaa cggggatcgc cgtaggtatg
     3961 gccacgaata tcccacctca taacttgtct gaggtagtag cggctatcga tttgctgatg
     4021 gataatcctg atgtgacgac caatgaattg atggaagtat tacctggacc agattttcca
     4081 acaggtggtt tggttatggg gaaatcagga atccgaagag cgtatgaaac aggtaagggt
     4141 tctattaccg tacgtgcaaa agttgagatt actgagatgc caaatggtaa agaacggatc
     4201 atcgtgacag aattgcctta tatggtcaat aaagcaaaat tgatcgaacg gatttctgaa
     4261 ctgcacagag acaagcgtat cgaagggatc acagatttgc gggacgaatc ttcccgtgaa
     4321 ggtatgcgga tcgtcattga catccgtcgt gacgccagcg catccgttat cttgaataac
     4381 ttatataaat tgacatcctt gcaaaattca tttggattca atatgttggc gatcgaaaaa
     4441 ggtgtgccga aagttttgag cttgaaacaa atccttgaaa actatatcga acatcaacgc
     4501 gaagtcatca cacgacggac ggtctttgaa aaagaaaaag cagaagcaag agcacacatc
     4561 ttagaaggtt tgcgtattgc tttggatcat atcgatgaaa tcattgcgat tatccgcggg
     4621 tctaaatcag atgacgaggc aaaagcaaca atgatcgaac ggttcgattt atccgatcgc
     4681 cagtctcaag ctattttaga tatgcgttta cgccgattga ccggtttgga acgggaaaaa
     4741 atcgaaaatg aataccaaga actcttaaaa ctgattgaag atttaactga cattctggca
     4801 agaccagaac gtgttacaga aatcatcaag aatgaattag cagacttgaa tcaacgattt
     4861 ggtgataaac gacgcacaga attactagtc ggtgaggtct taagtctgga agatgaagat
     4921 ttaatcgaag aagaagaaat cgttattaca ttgacaaaca atggttacat caaacgtttg
     4981 gcaaataatg aattcagagc tcaacgaagg ggtggacgtg gtgtccaagg aatgggtatc
     5041 cacaatgacg acttcgtcaa aaatctggtt tcttgttcta cccatgatac attgttattc
     5101 tttacgaata acggaaaagt gtatcgggca aaaggatacg aaattccaga atacgggcgt
     5161 acagccaaag gaatcccaat catcaattta ctaggtatcg attcgagtga aagcatccag
     5221 gcaatcattg cggtgacggg agaagcagaa gaaggccact atctcttctt tacgacgcgt
     5281 caaggtacag taaaacgtac ttctgtcact gcttttgcta acatccgaag taatggtctg
     5341 atcgcaattg gtttgaaaga agacgatgag ctcgtcaatg tgctactgac agatggtcaa
     5401 tctaatatca ttattggaac acataacggc tactctgtca ctttcaaaga agaagcagtc
     5461 cgcgatatgg gacgtacagc ttccggggtc agagggatac gcctgcgtga ggaagattat
     5521 gtcgttgggg cagccttaat gaaggaaaat caagaagtat tagtgatcac agaaaaaggt
     5581 tacggaaaac ggacaaaagt atcggaatat ccagtgaaag gccgcggagg taaagggatc
     5641 aaaacggcga acatcactga aaaaaatggt ccactagctg gattgaccac tgtttctgga
     5701 gatgaagaca tcatgctgat tacagataaa ggtgtcatta tccgtttcaa cgtatcgact
     5761 gtttctcaaa ctggacgttc aacattaggc gtacgtttga tgaagatgga agctgatacg
     5821 aaagttgtca cgatggcagc agtcgaacca gaagctgcag aacaaacagt tgaagaacaa
     5881 ggatcagaag aataaccatc aagactaagt gtgtaagcta gcaagaataa ccgattaaaa
     5941 aatctggtta ttcctgctgg cttttttgat tagttgaatt tctcttaaaa tattcgaaac
     6001 tgttcattct gtattgcttt tgaccaaaat agagtgtata attagagctt gtgagtagtc
     6061 taattaatta gataactctc cttgctctta ggaacctaag agccgaaagt ccacagggag
     6121 gtgaatatca atggaaaaca cgaaatatga aatcatgtac attattcgtc caaacattga
     6181 tgaagaagca aaaactgctt taatcgaacg tttcgatgca atcttgaaag ataacggagc
     6241 agaagttatc gaatcaaaag attgggaaaa acgccgtttc gcttatgaaa tgaacggtta
     6301 ccgtgaaggt atctatcaca tcgttaaagt atcttctcca tcatcagcaa atgctgtcaa
     6361 tgaatttgac cgtcttgcta aaatcaacga cgatatcatt cgtcacatga ttgttaaaga
     6421 agaagcttaa ttgttgtttc atgtgaaaca atagaaaacg atgagaggag atgactattt
     6481 tgattaataa tgttgtatta gttggacgtc tgacgaaaga tccagatttg cgttacacag
     6541 caagtggtac ggcagtcgca acatttactt tggctgtgaa tcgtaatttt acaaaccaaa
     6601 gcggcaatcg cgaggctgat tttatcaact gtgtgatttg gcgcaaatca gcagaaactt
     6661 tagcaaacta tgctcgtaaa ggaacacttt taggtgttac tggacgtatc caaactcgtt
     6721 cttatgataa tcagcaagga caacgtgtat acgtgacaga agtagtagct gacaacttcc
     6781 aattgctgga aagtcgttct gcatctgaaa atcgccaaca aagcggcgga ttccaaagtt
     6841 ctggtcaatc agctggcgga tttggcggaa acaataactc taatcaaaca tctcaatcat
     6901 ccaacggtat gcctgatttc gaccgtgata cttctgaccc attcggctca agttcaacga
     6961 tcgacatttc agacgatgat ttaccattct aaacgaattt gataggaggg aaatagaatg
     7021 gcacaacaaa gaagaggcgg acgtaaacgt cgtaaagtag actacattgc tgctaaccat
     7081 attgaatata tcgattataa agatatcgaa ttactaaaaa gatttatttc agaacgcggt
     7141 aaaatcttgc cacgtcgtgt aactggtaca ggcgctaaaa accaacgtaa aattactgtt
     7201 gcaatcaaac gcgctcgtat catgggcttg ctacctttcg ttagcgaaga ctaataaatc
     7261 ctaggaattg aatgactgat ccttttgatc agctattcaa ttcctttttt tctgtaaaaa
     7321 taacctctat tcgtttagtc gctcgaatga cgaaagttat ggtaaaatca aattagtcat
     7381 atatgggagg aaaattatgg aaaataaaaa caatcattta tccccgaaac ctctcttcct
     7441 tatcctgttt atattaatcg aagtattggt agtcttaatt ttaccatttc gttttgttag
     7501 cgcttcggtc attctattga tgaatttatg gggtgttcgc tattggttga aaatgaaacg
     7561 ttggcttatt ttaaataaac gtgaaaaaat ccagcaggct tcgactgtag ctgaaaccaa
     7621 tctagcttat gtctcagaag tcatgcctgt aggagtgatt gcttatcagc catcaagcca
     7681 aaaaatcgaa tggctcaatc cattagcatt gaaaatcaaa gatcaaagtg gattaacaga
     7741 ggaagaattg ctagatactt tttttactgc aaaagaaaaa aagaaaaacc aagtctatct
     7801 agcagataaa acatttcagt ttgatatgga tacagtaaag ggtgttgttt attttaatga
     7861 tattacccaa aaccagttgt taaaactcca tcagacgaac agtcagccgg tgattggtat
     7921 tttatctatt gataactatg cagatgccat tgataaatta gatgacaaag agatttctta
     7981 tttgaactct ttcgtcacta cactagtttc tgactggatg aatgaatacc atgtcttttt
     8041 caaaagaatc aacgcagaac ggtttttctt cgtagcacag atgatcgatc tgaaaaagat
     8101 gatggataaa aaatttgatt tgctggatcg ctttcgagca gaagctgaaa aacagagctc
     8161 gcctatcacc cttagtatgg ggattgctta tggctcaaga accctcgatg aaatcggtgg
     8221 agaagctcag aataatttag atgttgcttt agtccgtgga ggcgaccaag ttgtcgtgaa
     8281 agaggtaaaa gaaggagcaa aacctgttta ttatgggggg aaatctgcta agaatgtcaa
     8341 acgcacgcgt gttcgttctc gagcgatgag taccgctttg cgtcggattt ttgatgaaac
     8401 aggcgatgtg tttatcatgg ggcacaagtt tccggatatg gatgctttag gctctgcttt
     8461 tggtgtcagt tgtctggcac gcttcaacca gaaaaaagct tacatcgtta tcaatgagaa
     8521 cgagatcatt ccagatgtag aacggtgtct tgaagaaatc cataagcatc ctgaattaga
     8581 aatgcaacta atctctccag aacgtgccat agagctgaaa aaagaagagg acctcctcgt
     8641 gatggtggat taccataaac cttctctatc gatctctcag ggcctatatg agcaatttga
     8701 taagattgtg atcattgatc accatcgtcg tggggaagag tttccaagta aacctctgct
     8761 gacttatatt gaatcttcag cttcttcagc ttcagagcta gtttctgagc tgatccaata
     8821 ccaaagcagc aaaacaaatc gcttagatcg ttttgaggcc acattattgc ttgcagggat
     8881 ggttgtagat acaaatagtt tcaatgtccg tacaactgca cgaacttttg acgttgccag
     8941 ctatttaagg tcttgtggag cagacgcatc cttagtacaa tatttattga gctcggattt
     9001 agcgacatac ttggagatca gcaatttgat tgctggaagc gactatgtga cttctgatat
     9061 tgttgtggcg gcagggacag acgaaaaaga gtataatagt gttatagcag caaaaacagc
     9121 agatacattg ctttcgatga tcaatatcaa tgcagctttc gtcattacaa agcggacaga
     9181 cggcttgatt gggatcagtg ccagaagtac cggcacgatc aacgtccaaa tcatcatgga
     9241 atccttaggt ggcggaggac acttcacaaa cgcagctact caactggaaa atacgacagt
     9301 tgaggaaaca aaacagaagc tatttgctgc aatcaaagaa aatatgaatc aaatatatga
     9361 taaggagtaa tccaaatgaa agtaattttt ttacaagatg taaaaggaaa aggtaaaaaa
     9421 ggggacatca aagaagttcc agcaggttat gcacaaaatt atctaatcaa aaataacttg
     9481 gcaaaagaag caacgaaaag cagtatcagt cagttagcag gacaaaagaa agcacaagaa
     9541 aaacacgaag ctgaaatctt ggaagaagct aaaaagttaa aagaattctt ggaaaaagaa
     9601 gatactgtag tcgaattgaa ggcgaaagca ggggaagaca gccgtttgtt tggttcgatc
     9661 ccttcaaaac aaattgctga agggttaaac aaacaataca aaatcaaatt agataaacga
     9721 aaaatcgaat tagaaaatcc gatccgttca ctaggctaca caaacgtacc tgttaagctt
     9781 catcatgagg tgacggctac gatcaaagtt catgtcgtac aagaataaca aatcttgata
     9841 gcgatgttca gctggtaagt ataaccagaa ggaaaaaaga caaaccgaaa tttatctgag
     9901 aaaacagctg cagcaatgaa agcaaaagct gcaaatcgat ttcagattga tttcggtttc
     9961 ttttttggga acagcaaaat agcataaaga aaacgagtat aataaaagag aactattttt
    10021 ttattacact ttcggtacga atgatgactt ttctctcatt ttcttgtaaa atgaagcaat
    10081 tgaagacaag gagcgatacg tatgaatgaa gtgtggcagg atcgaatccc accgcaaaat
    10141 attgaagctg aacaagcggt tttaggctct gtttttcttg acgcagaaac gatcattgat
    10201 gccatggagt acatagagcc aaaagacttt tatcgtcgtg gccatcaaat tatttttgag
    10261 acaatgatcg agttaaatga tcgtaatgaa gcgattgacg tggttactgt caaagaccgg
    10321 ctggaacaag ccaatttgtt agaagatgcc ggtggtttaa gttatctctc agacttggca
    10381 ttagcagttc cgacagcagc aaatatcgtc tactatgcga aaatcgttga agaaaaatca
    10441 ttgctgcgta cattgatcca gacagcaaca ggaatcgtta cgaaaggatt cgagcaagga
    10501 gaagaagttc aaacaatttt agatgatgcc gaacgcagta tcttggaggt ttcggaaaaa
    10561 agaaatcgaa gcggcttttt atctattgct gaagtactga actccacgat tgaaaatatc
    10621 gatcgactag ctcaaaacaa tgaagaaatc acaggtctac caactggcta tcaaggtctg
    10681 gataaaatga cagcaggttt ccagcccgaa gaactaatca ttcttgctgc acgtccagcg
    10741 gtaggtaaaa cagcgtttgc attgaatatt gctcaaaatg taggaacaaa aacagatcgt
    10801 tctgttgcga tatttagttt ggaaatgggg gcagaatctc ttgtaaatcg gatgctttgt
    10861 gcagaaggtt cgattgaggc cagccattta cgtacaggtc agctttcgga agaagaatgg
    10921 tcgaatctga tcgttgcgat ggggagtctt tctcgagcaa atatttttat cgatgataca
    10981 ccagggatca aaatctctga aatccgtgcg aaatgtcgga aactagcgca agaaaaaggc
    11041 aatctaggct tgatattgat cgattatttg cagttgattg aaggtaacgg taaagagaac
    11101 cgtcaacaag aagtatcaga tatctctcgc caactgaaaa aactggcgaa agagctgaaa
    11161 gtacctgtca ttgctctttc acagttatct cgtggagtag aacagcgaca agacaaacga
    11221 ccagtgctaa gtgatattcg tgaatccgga tcaattgaac aagatgcgga catcgtagcc
    11281 ttcttgtatc gagatgatta ttatgaaaga gaaggcggag atgacgatga tggaaacgag
    11341 ccaaaaaatg acaacgtcat cgaagtcatc atcgagaaga accgaagcgg tgcacgagga
    11401 acagttgaac tactgtttat caaagaatac aataagtttt cgtcgctttc gccaagagaa
    11461 gaattttaaa gaagaggttg tgagccttga gcaatcccga gagctctcgt aagaagatgc
    11521 aaggagtaac cgcaggaacg actagagtac ttaatttaaa cagatctcca aagggattgt
    11581 gacaaaagtc ttgtcacagt ccctttttcc gaataaacgg tgttcaatca gctgaccctt
    11641 ggtattagct cagcaaacgt caaaatatga ggaactattt tgacgtttgt ttctctaata
    11701 ctcaggtcaa aacgctgatc ttacgacctc ttttccttaa acggtgttct cctgccaact
    11761 cctcggtcac aagtcacaat atcatcaaac atgagaagca gttttttgaa attgctcctt
    11821 gtgactcaga gttaaacggc aggctcacaa cctctttctt aaacggtgtt caaaagcttg
    11881 catctgcggt aatacgccca atcaaacgaa aaatatgaga agctattttt gtttggctgt
    11941 tcttattact tgatgcagga cagctttttc acaacctctg attcacggtg ttccaaaagt
    12001 agcttcttcg aaaaataagt cgaaatttca caaaaatttg agaagcaatt ttcgtacatt
    12061 ccttcttatt tcttgaagct gaccacttct gttacaacct cttttttaat aaccattcta
    12121 aaaagataaa gttcgttttt taagcgtttt tataaaataa aaataaaaat cattcgattt
    12181 tatttgaaat atgtatctat tattgataca atggttcagg attaaataat atagaacgag
    12241 gtgttcacat gtcatctgta gtagtagtag gtacgcaatg gggcgatgaa gggaaaggaa
    12301 aaatcacaga ctttctcagt gaaaatgctg aagtgattgc acggtaccaa ggtggcgaca
    12361 atgcagggca cacaattaag tttaacggtg tgacttataa actgcatttg attccttctg
    12421 ggatttttta taaagacaag atcagtgtga tcggaaacgg cgttgtagtc aatcctaaat
    12481 cattggttaa agaattagct tatttacatg aaaaaggtgt agaaacaagt aatttgcgta
    12541 tttctgaccg cgcgcacgta atcttgcctt atcatatcaa attggaccaa ttacaagaag
    12601 atgctaaggg cgaaaataag attggtacaa cgattaaagg aatcggacct gcctatatgg
    12661 ataaagcagc tcgtgtaggt atccgtattg cagacttatt agataaagaa atttttgaag
    12721 aacgtcttcg tttgaatcta gatgacaaaa accgtcaatt tgtaaaaatg tttgattcag
    12781 aaccacttga ttttgacgat atttttgaag aatattatga atacggacaa caaatcaaac
    12841 aatacgtcac agatacttcc gttatcttga atgatgcttt ggatgcaggc aaacgcgtgt
    12901 tgtttgaagg cgcacaaggt gtaatgctgg atatcgacca gggaacttat ccttttgtta
    12961 cttcttctaa cccagtagcc ggtggggtaa caatcggaag cggtgtaggg ccttcaaaaa
    13021 tcaataaagt tgtcggcgtc tgcaaagcct atacgtcacg tgtcggtgat ggaccatttc
    13081 caactgaatt atttgatgaa acaggagaac aaatccgcga agtaggacga gaatacggta
    13141 cgacaactgg tcgtccgcgt cgtgtaggct ggtttgattc tgtagttatg cgccattcta
    13201 aacgagtttc aggtattacc aacctatctt taaactcgat cgatgtatta agtggattgg
    13261 aaacagtaaa aatttgtacg gcttatgaat tagatggcga actaatctac cactatccag
    13321 caagcttgaa agagttgaac cgctgcaaac caatctatga agaattgcca ggctggtcag
    13381 aagacattac aggatgcaaa acacttgcag aactgccaga aaacgcacgt aactatgtac
    13441 gtcgtatctc agagcttgtt ggtgtacgta tctcaacatt ttctgtcgga cctgatagaa
    13501 cacaaacaaa catcttagaa agcgtctggg cgcaaatcta aggataaaaa cgacgactga
    13561 atcaggacgt tcttaccaga gcgagtcgaa gtgtttatct atacgataat aataacttga
    13621 agaggtagtg aaagagacgt ttagccttat tgccgatagg ctgtctcttg aacactgttt
    13681 attcagaata agggactgtg acaaaaac
//
LOCUS       DAZAID010000073        13400 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000073 DAZAID010000000
VERSION     DAZAID010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.073"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG9BE_002077"
     CDS             99..746
                     /locus_tag="ACG9BE_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFN3032813.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG9BE_002078"
     CDS             1139..3784
                     /locus_tag="ACG9BE_002078"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFN3032814.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG9BE_002079"
     CDS             4094..5407
                     /locus_tag="ACG9BE_002079"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFN3032815.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTEAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG9BE_002080"
     CDS             5397..6050
                     /locus_tag="ACG9BE_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3032816.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG9BE_002081"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG9BE_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFN3032817.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG9BE_002082"
     CDS             6947..7147
                     /locus_tag="ACG9BE_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3032818.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACG9BE_002083"
     CDS             complement(7401..9080)
                     /locus_tag="ACG9BE_002083"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN3032819.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACG9BE_002084"
     CDS             complement(9082..9294)
                     /locus_tag="ACG9BE_002084"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFN3032820.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACG9BE_002085"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACG9BE_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFN3032821.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACG9BE_002086"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACG9BE_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFN3032822.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
BASE COUNT         4235 a         2333 c         2887 g         3945 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag ttgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgaagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca
//
LOCUS       DAZAID010000074        13399 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000074 DAZAID010000000
VERSION     DAZAID010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.074"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(348..1220)
                     /gene="scrK"
                     /locus_tag="ACG9BE_002087"
     CDS             complement(348..1220)
                     /gene="scrK"
                     /locus_tag="ACG9BE_002087"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN3032823.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(1484..3430)
                     /locus_tag="ACG9BE_002088"
     CDS             complement(1484..3430)
                     /locus_tag="ACG9BE_002088"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFN3032824.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            3615..5054
                     /locus_tag="ACG9BE_002089"
     CDS             3615..5054
                     /locus_tag="ACG9BE_002089"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFN3032825.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            5056..6018
                     /locus_tag="ACG9BE_002090"
     CDS             5056..6018
                     /locus_tag="ACG9BE_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN3032826.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(6188..7614)
                     /locus_tag="ACG9BE_002091"
                     /pseudo
     CDS             complement(6188..7614)
                     /locus_tag="ACG9BE_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            7857..8312
                     /gene="tnpA"
                     /locus_tag="ACG9BE_002092"
     CDS             7857..8312
                     /gene="tnpA"
                     /locus_tag="ACG9BE_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFN3032827.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            8577..8675
                     /locus_tag="ACG9BE_002093"
                     /pseudo
     CDS             8577..8675
                     /locus_tag="ACG9BE_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(8898..9128)
                     /locus_tag="ACG9BE_002094"
     CDS             complement(8898..9128)
                     /locus_tag="ACG9BE_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFN3032828.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(9358..10176)
                     /locus_tag="ACG9BE_002095"
     CDS             complement(9358..10176)
                     /locus_tag="ACG9BE_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN3032829.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            10337..11026
                     /locus_tag="ACG9BE_002096"
     CDS             10337..11026
                     /locus_tag="ACG9BE_002096"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFN3032830.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            11040..12542
                     /locus_tag="ACG9BE_002097"
     CDS             11040..12542
                     /locus_tag="ACG9BE_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3032831.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            12555..13031
                     /locus_tag="ACG9BE_002098"
     CDS             12555..13031
                     /locus_tag="ACG9BE_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN3032832.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(13128..13238)
                     /locus_tag="ACG9BE_002099"
                     /pseudo
     CDS             complement(13128..13238)
                     /locus_tag="ACG9BE_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4390 a         2242 c         2360 g         4407 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga acctatttgt taaaaaggtc
      121 caaaaaaata tccgcacccc cttttttttg taaaaaacta ttgcacaata caagtggcct
      181 acggctatcg aaatttccat aactttcgag caagaattta tctcattcaa ggcttgatct
      241 tccaagtaaa acaaaaacca gttaagcact ctgcctaact ggttatgact aaattttcag
      301 ttgtatcttt ctctaccaac actaaatgtc gaagagcctt ttttcgttta tgaatgtaag
      361 accgcttctc gagccaatgc caaacaaccg agcgttccag cgttgtcttc taatgctggg
      421 gtcacgatat atttgcttaa ctcaggtgtt tcgacatacc cattgaccaa ttttgcaaat
      481 gcgtcgtgga cgttttgcag catatggcgt tgtttcatca caccaccgcc aaaaatgatc
      541 acatctggag aaaacatcaa ggttgtattg taggcacatt gagcaatata ggaggcttcg
      601 atttcccaat aaggatcatc ttctgcaacc tcttgtcctt ttttccctaa gcggttttcg
      661 atggcgggtc cagcggccat cccttctaaa cagttgccat gaaatggaca gctgccagca
      721 aagtcatctt ttggatgagg gacgacgagc atatgcccca tttctggatg actaaaccct
      781 tcgataaagc gcccttcttg taaggcaccg ccgccaacac ccgtaccgat cgtatagtaa
      841 acgacactgc tcaatccttt gccactgccg aagacatact caccataggc agccgcattg
      901 acatctgttg tccaagaaat cgggatcgga aattcttttt tcatggtacc gatgaaatca
      961 aaattttgcc atgccaattt cggggtcgag gtaatgtacc cataagtggc ggaatcgcga
     1021 tggatatcga taggcccaaa tgagccaatc ccaatccctg ctaaatctgc ttggtattgt
     1081 ttgaagaagt caatgactaa aggcattgtt tcctcaggtg tcgttgtcgg aaagctcact
     1141 ctttccttga tcgtcatttc ttcatcgcca atggcacaca caaattttgt gccgcctgct
     1201 tcaattgcac cgtatagcat agacatttct ccctttattt tagaatgaaa tcactaaata
     1261 aatgactata tatcatattt aaattatttt tttatttagt aacatgacaa gtaaataact
     1321 actatttttt ctttttgaat ttttatcatt ttttaaaatt ctaataattt ttcatacaat
     1381 tgatatatga aaagattaaa taaaatgtta ttctcaaata tttgattaat ctatattatt
     1441 tgattttgga aagaaaattc catctttact cagggaaata tatttatttg atttcaagta
     1501 aatcagtacc ctcagtgact gtattttcag ctactatatt tacttctgaa taatccgaag
     1561 tattggtcac aataatcatt gttgtatcat caagaccttc tttctcaatc attgaacggt
     1621 caaatgctcc aagaatatcg ccttttctaa ctttttgatt agtagtaact ttttgtgtaa
     1681 atccttttcc tcccattgat acagtatcaa taccaatatg aattagaact tcagcaccat
     1741 tatttgattt aataccataa gcatgtccag tttcaaacgt gagttgaata gtaccatcta
     1801 ctggagaatg gaccgtgttt ccatttggtt taatggcaat cccttttccc atagcttctg
     1861 atgaaaagac tggatcattt actgaagaaa gactaatcac tttaccagaa atgggactaa
     1921 caattgtttc tgatgaaact tttgtttgtg ttgattcaac atcagtagat attggatctg
     1981 ttgttgctac ttttgaagaa ttaactggtg tttgttcagc aatttcttct gctttaaaga
     2041 taccagcagc ttttgtttta ccgtagaagt aagtaattgc aaaagcaact acaaagctaa
     2101 tcaattcaca caatacgtaa aatggaatag atgttgcatt aattgaaaga aatcccaaga
     2161 aacctgctga accaagtgaa acagcaacta ctttaagtaa tcctgctact gctgtagcaa
     2221 ttcctgaacc aattaaagca caaaagaatg gaaagcggta ttttaagtta actccgaaca
     2281 aggcaggctc tgtaattcca agaagtgctg agacacccgc agaggaagca agactttttg
     2341 tcttagaatt tttagttaag aaccaaatgg caaatgttgc agcaccttga gcaacgtttg
     2401 ccatagatgc aactacaaag ataaagtctc catgtccgat accatttgtc cacgctgaaa
     2461 gtaattgagt ttcaatagct ggaaatgatt gatgtaaacc agtcatgact ataagtgaat
     2521 atgttcctcc aaaaataccc attccaataa atccagtagt gttatatagc caaacaataa
     2581 tgtctgtaat tccattagac aacaataaca ttacagggcc aataactgtg aaagtcaaaa
     2641 atccagtaat cattactgat aataatggcg tgaatgtaaa gtcaatagat gatggcaatt
     2701 ttttatgaaa gaatttttct aatatagata atatccaaac agctgctaaa actggaataa
     2761 cttgataagc atagtttgtt tgtgaaacat gataaccaaa aatattccaa tattcagttg
     2821 agccaccaag atctggtgtt gtcatgatca taccaattgc agcaccaaga aattggtttg
     2881 ctccaaaacg tttagcagct gaaataccta ctaaaattgg taagaacatg aatggtgcag
     2941 cggacatcag ttggatcatt gaggaaatac cttcaatcgc aggatacatt tcttcaattg
     3001 attttggtcc aaaaagtcca gcagctgtta aaaagttttg taatgccatt aataaaccac
     3061 ctgcaacaag ggcaggtata attggaacaa aaatatctga taataattta attaatgcca
     3121 taatggggtt gaatttttta tctttactag caatttgttt taaatcatct gttgaaattt
     3181 ccgaaagtcc agtttttttg ataagttcgt cataaacgaa attaacatca cctgcaccga
     3241 taatcacttg atattgtcca tcaattttaa atgtgccttt cacatctgga ttttcgtcta
     3301 aagctttttg attcactttc gtgtcatctt ttagtacgag acgaagacgt gtagcgcagt
     3361 gtgcagctgc aataaggtta tcttttccga ccgcttgtat tacgtcggaa gctactttac
     3421 tgtaattcat tcgaatcact ccttttttat ttttacgtga aaacggttca ctgaaatgca
     3481 aatttatttt agcatatctt aaaaatatgt caaacgtttg tcatatagtt tattttttta
     3541 aatgaaatga ttgatttttc aacggaaaac gtttaccata ctagttgtaa aaagataaat
     3601 tgaaggagta aaatatgaac ttacctcgag aaatacgata tcgtgcttat tctgattgga
     3661 ctaaagatga gatggataaa attaaggata atgtaaaacg atcaccttgg cgtgctagct
     3721 atcatatcga acctaaaaca ggattgttaa acgatcctaa tggtttttct ttttttaacg
     3781 gaaaatatac gcttttttat caaaattggc catttggagc tgctcatggg ttgaaagaat
     3841 gggtacacaa tgaatcagat gatttagttc attttcatgt aacaggagca gaacttcttc
     3901 cagacacaaa acgagatagc cacggcgctt attccggttc tgcatatgaa atagagggga
     3961 atctattctt actctataca ggaaacgttc gaaatgaaaa ttgggaacgt gatacacttc
     4021 aaatcggagc atggatggat aaagactaca acattacgaa aggtgagaat gttcttattc
     4081 ataaacctgc tgatgtaact gatcattttc gtgatccaca aatgtttaat tatgaaggac
     4141 aattgtatgc gattgttgga gcacaaaatt tagaaaaatc aggctttatc aaactttaca
     4201 aagcggatga caataacgtg gaaacatggg aggaagttgg caaccttgat tttggcggca
     4261 ctggttcaga atacatgatt gagtgtccaa atctagtatt tgtagacaaa aaacctgttc
     4321 tcatttattg tcctcaaggg ttggataaat ctgagctaaa ttatgggaat atctatccga
     4381 atacttttaa gatctgtcaa tcgcttgaca tagaaaaagt aaaacttgtg ggcgcttcag
     4441 aaattcaaaa tcttgattat ggatttgaag cgtatgcgac tcaaggattc aatactcctg
     4501 atggtcgtgc tctgattgtc agttggattg gactacctga tatcgattat ccaactgata
     4561 agtatgatta tcagggagca atgagtcttg taaaagaact ttcaataaaa gatggtaaac
     4621 tttaccaata tccagtagaa gctattacat cattacgtgc tagttctgaa caatttacag
     4681 caaaagcaga aacaaataat acttatgagc ttgaactaac ttttcctcca aatcagaaat
     4741 cggagttact tcttttctct gatagtaaag gaaatggact tagcttgatt gtagatacca
     4801 aagaaggaaa aattatttta gatcgcagca aagcaggcat tcaatatgcc actgactttg
     4861 gtacgattcg tgaatgctca attgatacta aagaaacaac tgctaacatt tttgtagata
     4921 actcaatcat tgagattttt attaataaag gagaaaaagt gtttactagt cgtgtattcc
     4981 ctgaaaatca ccaaaatggt attcaaatca aatcaggcga acctactgga aaatactttg
     5041 aattaaaata ttaaaatggt agcaaaatta actgatgtcg ctgaattagc tggagttagc
     5101 ccaacaaccg tttcacgcgt cattaataat aagggatatt tatcagaaaa aacaaaacaa
     5161 aaagttcatg aagctatgaa aactcttggt tataaaccaa ataatttagc acgtggactt
     5221 caaggaaagt cacctcagct tattggtctt atttttccta atattagtaa tatcttttac
     5281 tctgaactca ttgagtatct cgaaatagag ttatttaaac atggatataa aacgattatt
     5341 tgcaatagtc aaaatgatcc tgctaaagag agagaataca ttgaaatgct tgaagccaac
     5401 caagttgatg gcataatctc atcaagccat aatcttggaa ttgctgatta tgagcgtgtg
     5461 agagcaccta ttgttgcttt tgatagaaat ttagctccta atgtttccat tgtttcttca
     5521 gataactttg aagggggaaa acttgcagct aagactttac aaaaaaatgg ttgtcaaaat
     5581 accattatga ttacaggaaa tgataacacg gattctccca cagaattacg tgcactaggt
     5641 ttttcttttc aaaatccaaa tggaaaaatt ttcaaaattc ctaataatct gtctactatc
     5701 cgtcgtgaaa tggaaataaa gtcaactatt ataaatcaca aaccagatgg tatttttgtt
     5761 tctgatgatt taacagccat tctcactatg aaaattgctc atcaacttaa acttaatatc
     5821 ccagaagatt taaaaattat tggatacgat ggtacctctt ttattgagaa atttttccca
     5881 caattgacaa caataagaca accaattgat gaaattgcaa gtcttattgt tgatattctt
     5941 ctgaaaaaaa ttaaaggcga aaagacaaat aaggattata ttttaccaat ctcaatactt
     6001 tctggaggaa gtatttaacg ttaaattaat tttttaatgt ctataaaatt cgttagaaaa
     6061 tacaatttgt agatatccaa ttgatttgta aacaaaaagg agttgtcaac ttgtagtgac
     6121 ccccaaaagt tagacttttt tggagtggag aaatccactc cttttttcat gctctaaaat
     6181 gatattttta cacaactgtt acggttgttt tacgaaattg gacgggactt tgccagttta
     6241 atttctgttt tatccgttca ttattgtagt aatagatata gctatcgatg gctgatttta
     6301 attcctcgaa acagttataa actttcccgt gaaaaatctc ttgttttaat aagccaaaaa
     6361 aattctccat aagtgaatta tctaagcagt tccctttccg agacatgctt tgaaagattt
     6421 tgtactcctt caatttgttt ctataagctt tcatttgata cgcccaacct tgatctgagt
     6481 gaaacgtacg acgataggga caatcgttag tggtctggat agcttcctcc aaaccttcca
     6541 ttaccgctaa tgcattgggc ttttcagaga tacgatagga taaaatctca ctattgaaca
     6601 tatccataaa aggatctaga tacaatttct tttgacggat aatgcctttg gcatccactt
     6661 catggtattt aaattcagtt gtatccgtcg tgattttctg atggcagata ctggtgtaga
     6721 aacgacgatg gatccgattt ttggctactg ctccgacttg tcctcgataa gaattgtagc
     6781 gccgtgattt acgtgtgtat gtcgttactt ttattcctag cttcttgatc aggcgctgaa
     6841 ctttcttttt attgatagag aaaccgcggt ttcttagttc attggtcatc cgtaaacagc
     6901 cataatcctt gtgcttctcc cgaactttaa gcatttccgc ttcaatctct tgatctggat
     6961 ttttacggtc caatcgtttt tgccagtaca taaagattgc cttagggaac tttaaagttt
     7021 ctagaagctc aactaattta aacggtcctc ggaggcttgc gatgattcgt gcgattgctt
     7081 cattcgtcgt tgttgggctt cctgttttcg cagcttcctc aattctttta aaaaggcatt
     7141 ttcaatttga agggaacgga ctcgtttctc caactcctcg atgcgttcac gttctttaga
     7201 cgttgcttta gttgtagatt ttttcttatt tttgggttca tttttcttag acaagatagg
     7261 tggacatcct ttcgtttttg agagtccatc gatcccaccc tcacggaatt gacgcatcca
     7321 actagcgatc aaattagggt tgttcatgtt aagtgtgttg gcaacttcac gataggacag
     7381 ctcacttgtt agatataact ctatcgcatc cagcttaaat tgaacagaat attttttctt
     7441 ttgtcgcttt cttactagac cctcttcacc aaattcttga taggcattga tccagcgctt
     7501 cacttgtgat ggagccttaa tggaatattt ctttgccaga taatcacttc cgcctttacc
     7561 atccaagtaa tcatgaacga tctttaattt aagttcaaaa gtatacttag ccataaaaaa
     7621 taccgacctc caaaagttag attttttggt ctaacttttg ggggtcggta caaaacttaa
     7681 aagttttaga cccttttatt ttcttaatga cttcagtcag ttttgtgcgt aaagcgtgcg
     7741 aaacaagaaa accgcgcgtt tctacgcgtg gaagaagtat ctttagcgat aaaaactcct
     7801 ttcgatatac tagtgagtgg ctaccaaccg ctcatcagat cgaaaggagt tttttcatgt
     7861 caaatgacga taaaagttta gcatatactc gttggaattg taagtatcat attattttta
     7921 caccaaaata tcaacgaaaa gtaatctatg ggcaattacg aaaagatctt ggaaaaatac
     7981 ttaggaagtt gtgcgaaatg aaagatgtgg aaatcataga agcacatgca atgctagatc
     8041 atattcacat gcttgttcga gtaccaccaa aaatatgtat atccagtttt atggggtatc
     8101 tgaaaggtcg tagtgcggta ctaatccacg agcagcacgc aaatctcaaa tataaatacg
     8161 gaaacaaatc cttttggtcg aagagatact atgtgagtac agtcggattg aatcaaaaga
     8221 caaatgaaaa atacatccgt gaacaagaag ctgatgatcg cattcgatat attataagta
     8281 aaagagagta cgtagaacca tttaaaaaat aaagagaagt aagtgagtga agatgagcag
     8341 ttggcggtct tttaaaaatc cccttttaag gggcagcgag tgtcgacccc ttctaggggg
     8401 attaaaaata cacccgtcct cacgggtgta tgggttactg atagacttga ttttaactgg
     8461 tgtcagaaac ttatgtccag aaagggtgat gaaattttgg caatttacgg ctattcaaga
     8521 attagcacaa gcaaccaaga ttacaaaaca caaattcaaa cattagaaaa tgctggcgca
     8581 gaaataatct tcctagaaaa tcactggatt atcgaacacc tttggaagta tttttgagtt
     8641 acgtaagtat tgatgatctg tctaacttaa tttgacaatt aagagtatat ttacttcaga
     8701 tggtaaggca acaaaataaa atacaatagg cagatgaact aataaataaa atctgtatta
     8761 aatacagata aattaatata aaaatcaaaa ggatcataaa cttttaacaa gtgatgaaat
     8821 gggtggaaat tgattcttat agacagtttg tctcataaca ttgaagtatt ttgcaatttt
     8881 agcattgaaa cactttttta atcttcaacg ttgttcttat aaacaagatg attgggaatc
     8941 ttttgaatta acactgtata gcttagaaca ccctttatag aaaacattcg cttagcaatc
     9001 ttaataattc cttgtgcccg atgtcgttta gattcagtag gttcctgctc atcgatcatg
     9061 gaaaaaattt gaaggatcag atccttaata aatttattta agagaggatg atccattgct
     9121 tcatccatac tcgataaact agtgataatg agctacacgt ttttctttca agttgatcaa
     9181 tagcttccac gataaaaata tctttagata aaatcaagaa ccgtaggaac tacagggata
     9241 tttggtcaat aagagccacc gctattagta aaaaataagc tatcaagtat gctctcatcc
     9301 caagaattgc ctacttcaat cgttaaaaac cgttaggttt agctaagtgg gggtagttca
     9361 ctcaatactt ttatcaataa tagagcgcaa aactttttct cttaattcaa ctgaatcaat
     9421 attattgttt tgttcataac catgaactaa tagatcacaa atataaagtt gtgatatttt
     9481 tccagctaaa gatccaccat taagaaattc ttcaatggct gtttgtaaaa ctaagtctgc
     9541 agactttcct ataggtgaat gaatatagtt agttatagca ataatttttg cgccattttt
     9601 tttagctatt ttcaatgaat cataagtatc tttcgttttc ccagataaag aaaaaatgat
     9661 tactaaatca ttaactgtta ataaagaagc gacttgggct tgaaaatgag ggtctagtac
     9721 agcttttgct tgaactccta cgcgaagaaa catattttct aaatcaagac tagtatttcc
     9781 actagatccc actccaaata tatatatgtt ttttgattga gtgatcattt taatggcttc
     9841 attcaatttt tcttcgtcta aaagcctaat agtggaatag agagcttctg ttaaactatt
     9901 agcaatttca tcatagtact ttacactagt cggatgttct tttttttgac taaagtcttc
     9961 ctttgcaatt tcaattttta aatcagaaaa accagaaaaa ccaatttttt gacaaaagcg
    10021 aataatagta gcatcgccaa cttttgtgtt tttcttaata tcactcatcg tactataaat
    10081 aatagttttc ccagaattta aaataaaatc agcgactttt ttttcagact tcgttaaaaa
    10141 tggataattt tctttaatta aatagatata actcatacaa actcctctac aatttcaact
    10201 tttctccatt atactccaaa ataataaaaa aaactcctaa atactccatt tttatcttta
    10261 cttttatgaa agcggatgct atattgtttt tggagttgtt atccaaaaat aataaaaaat
    10321 tggagtgttt aggataatgg ataaaaagag gtttttagat cttatttcaa aaggtttgat
    10381 tgtttcctgt caagcattac ctggtgaacc attttacaaa gaggaagaaa gtttaatgcc
    10441 ttattttgct ttggctgctc aaagggctgg agcagttggt atacgtgcaa attctgttcg
    10501 agatattatt gaaattagaa agtgtgtatc attaccaatg attggaatta ttaaaagaga
    10561 ttatccacca gaatctccat tcattactgc cactatgaga gaagtagatg aattagtaga
    10621 aaccggtgtt gaggtaatcg ctcttgattg cactcttaga aatcgacatg atggactatc
    10681 tataaattct tttattacta aaataaaaga aaaatatcct catcagttat ttatggcaga
    10741 tatttctaca tttgaagaag gcaaaaatgc atataaagca ggaatagatt ttgtaggaac
    10801 tactttaagt ggatatactg aggaaagtaa aaaacaagat gggccagata tagatttgat
    10861 taaaaaattg gtagaagaag ggatttgtgt aattgctgag ggaaggattc atactccaga
    10921 acaagcaaaa gagataaaaa aaataggagt tgcaggtatt gttgtgggtg gtgctattac
    10981 tcgaccacag gaaattgcat caagatttgt taaagcatta agttaggggg agaaataaaa
    11041 tgtttaaaaa attttcacaa ttaggacgcg ctttcatgtt accaattgct atattaccag
    11101 tagccggatt actattggga ctgggaggag cgttaacaaa tgagagtgct ataaatgctt
    11161 atccattttt agatcaaccg tggttgcata caattttaag tattatgagt tatgctggaa
    11221 atgcagtttt tgcaaaccta gcactaattt ttgcaattgg tattgctgta ggacttgcaa
    11281 atggagataa aggaacagct ggtttagctg gtggagtagc ttatctagta tatactgcaa
    11341 ctattagtgg gtttcttgaa cttttttcag caaaagatgc aacactagat acaggtgtag
    11401 tgggatcaat tgttgttggt ggtactgttg catttttaca caatcgttat agaaaaattg
    11461 aattaccaca attcttaggt ttttttggag gatcaagatt tattccgatt gtttctagtt
    11521 tagctgcaat cattattggt tctttctttt atttaatttg gccaccaatt caaaatggtt
    11581 tagtagttgc tggtgaacaa attgcccaaa tgggtagttt aggaactttt ttatatggtt
    11641 ttttattgcg tttaacaggt gctgttggac tacaccacac catttaccct ttgttctggt
    11701 atacatcttt aggtggtacc gaaactgtag ctggatcaac aattgctgga gcccaaaata
    11761 ttttctttgc ccaactagct gatccaaacc acactggtct gtttacttat ggtactagat
    11821 tttttgcagg aagatttgca acaatgatgt ttggattacc agcagcatgt tatgcaatgt
    11881 atcgtgcaat tcctaagaaa aatcgtaaaa agaatggtgg gctttatttt agcggggccc
    11941 ttacatcatt tttaactgga attactgaac cagttgaata tatgttttta tttgttgctc
    12001 cttggcttta tgtgattcat gcattcttag atggattatc tttttatttt gctgatatct
    12061 taaatattcg tgtagggaat tccttttcag gtggtttgat cgacttttta ttattcggtg
    12121 tacttcaagg gaatgacaaa actaattggt taaaattaat tccttttggt attgcatggg
    12181 catttatata tttctttgtt tttacattct ttatcaaaaa atttaaggta gctattcctg
    12241 gaatggaaga agaatcagaa gttcattctg aaaaagtaat aaacaaaaat acatcattac
    12301 atgaagagtc tcttatcatt attgaagctc ttggtggaaa agaaaatatt gagtctgttg
    12361 aagcttgtgc aactcgtttg cgtgttgctg ttacgaatgg ggagattatt caaaaacaaa
    12421 caataaaaga cactggtgca actgctgtct tcgaagtgaa gggtggcatc caagctgttt
    12481 ttggagggaa agctgactta ttaagtcaag aaatcaatca aatattagga aaagataatt
    12541 aggaggtttt taatatgttt gaaattttca aaaagaaaaa acaagaaact ttttatgcac
    12601 cttgttccgg aaaaatcatt tccatcacag aagtaaatga tcctgtcttt tcacaaaaat
    12661 tactaggtga tggttttgcc gtcatacctg atggttcaaa aatttatgct ccattggctg
    12721 gtcgagtaac aaatatattt cctactaaac atgctattaa tataaaaagc aactcaggca
    12781 ttgattattt gattcatatt ggaattgata cagttgaatt acaaggagag cctttttcga
    12841 tttgtgttca agaaaatgat gaggttactc ctgaaacctc gttggttgat gtggattttg
    12901 acaaaataat aaaatctggt aaagatactt ctgttatagt tgtttttccg gagaaaaatc
    12961 agttcaatga tttaataata gataatggtc gaattgctca tgggaattca tgtggaaaaa
    13021 tcacttttta aaaaagtgta tataaaaagc tataaaataa gctaaatagg atatttgtca
    13081 aatgaagttg gtgaacagaa agaactgttt tctttctgtt cattttttta ttttattagt
    13141 aatgattccc catgccaaat catgatccgt aatttaaagt tataaaagct tcgaaatcca
    13201 ttggcatttc gttggaatac cttgatatga ttattcaaat ataaaatata gagagaagag
    13261 agagagggaa gatatttata aagggcaaag ttatgtttac tcaacttagg aaatagaaat
    13321 agatcaattt atattctctt gtcaatcgaa tatgaacctt agcactatat accaaatata
    13381 gtaaaatgct gaaagatgt
//
LOCUS       DAZAID010000075        13345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000075 DAZAID010000000
VERSION     DAZAID010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.075"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(497..1111)
                     /locus_tag="ACG9BE_002100"
     CDS             complement(497..1111)
                     /locus_tag="ACG9BE_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN3032833.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1717..2094)
                     /locus_tag="ACG9BE_002101"
     CDS             complement(1717..2094)
                     /locus_tag="ACG9BE_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032834.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2394..3275)
                     /gene="galU"
                     /locus_tag="ACG9BE_002102"
     CDS             complement(2394..3275)
                     /gene="galU"
                     /locus_tag="ACG9BE_002102"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFN3032835.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3318..4337)
                     /locus_tag="ACG9BE_002103"
     CDS             complement(3318..4337)
                     /locus_tag="ACG9BE_002103"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFN3032836.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4970..5974)
                     /locus_tag="ACG9BE_002104"
     CDS             complement(4970..5974)
                     /locus_tag="ACG9BE_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN3032837.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5987..7036)
                     /locus_tag="ACG9BE_002105"
     CDS             complement(5987..7036)
                     /locus_tag="ACG9BE_002105"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN3032838.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(7053..7874)
                     /locus_tag="ACG9BE_002106"
     CDS             complement(7053..7874)
                     /locus_tag="ACG9BE_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3032839.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7861..8640)
                     /locus_tag="ACG9BE_002107"
     CDS             complement(7861..8640)
                     /locus_tag="ACG9BE_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN3032840.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8657..9127)
                     /locus_tag="ACG9BE_002108"
     CDS             complement(8657..9127)
                     /locus_tag="ACG9BE_002108"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3032841.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(9139..9558)
                     /locus_tag="ACG9BE_002109"
     CDS             complement(9139..9558)
                     /locus_tag="ACG9BE_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3032842.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9639..12416)
                     /locus_tag="ACG9BE_002110"
     CDS             complement(9639..12416)
                     /locus_tag="ACG9BE_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN3032843.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12607..12831
                     /locus_tag="ACG9BE_002111"
     CDS             12607..12831
                     /locus_tag="ACG9BE_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032844.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3936 a         2771 c         2158 g         4480 t
ORIGIN      
        1 atttcattgc aaaaaaaata ataaacactt ataaatatcc attatctatc cttcaatttt
       61 tgtgtatttt atttctatta gaaaaaggta tcctgttgga caattttgtt atatcaggta
      121 aacttcattg caataatgat aacaaatgta ctataattac tttgtaaaga gttttgggga
      181 agactctttc atcaacgtgt attaagagac aagaaagaat ccatatggat tctttcttgt
      241 ctctttttcc actacttttt atttttctga cactacatat tcttataact catttctcat
      301 aactgtgaaa caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga
      361 tgatccatct taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat
      421 cgttttcttt ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt
      481 gtcaacatgt ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat
      541 acaaaacttg ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat
      601 ctagatttgg caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat
      661 cagcaaattc aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat
      721 caaatccagt aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga
      781 tgattgccgg gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag
      841 aaagctgatc aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg
      901 gattttcaat caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta
      961 caccacttag tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg
     1021 cgatccaagt gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt
     1081 ggtgataata gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca
     1141 tgaatagaaa cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa
     1201 cacatgtggt ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc
     1261 ttgacatagc aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt
     1321 ttcaccctag attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg
     1381 atcggtcagg gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt
     1441 cagggagcct ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc
     1501 caagtgcaag caagaaaata caaaaaaaca ttggtatgat ttatatatat ataaataaca
     1561 taaaaaaagg aatggcttat cccgatacaa caagcattcc tttaaaaaag attcttttat
     1621 ataagtgaaa aaagcttttt tccaccaatt aaataaatct ctcatctcaa tagtttatcc
     1681 attattttat ctttttttgc gtatacctac taaatattac cgtcttcttc tcgttcttct
     1741 actttgttcg agagaatctg tactagaaaa agtagttgta tgtctacctt ttctgcctat
     1801 ccgagacttt cttttcatat atgggatata aatgattaga tacagtacag caaaaaataa
     1861 agcaaatacg caagcttccc aactacctaa cgccaattca accatcaagg tagcaacccc
     1921 aacggatgct gcataccaga taaaaatcag aaacgtttct ttttttctga ccatgcgaaa
     1981 catgataaat agactaataa gcaatagtgg caacacaaat atccaattgc ttccttccat
     2041 cagccacttg agtatatcaa gcagcgactg tgcaacagcg actaattttc ccatagtgaa
     2101 cacacacctt tttttataat taaaaaattc gcctttttat tctactatga atcttctggc
     2161 ttggctttca tttctcttat gtaatcattt tcttttatac aacttaaaaa aaaattaaac
     2221 cattagcata atattttctg aatctattca cgaaagtctt tttctatctt tcgttttcca
     2281 cgactcaact gatctagagt caaaaaacta aaacgacagt tgcgcaaatg acgcacaagc
     2341 tgccgcttta ataacaaata gcctttcatt ctcagcaggc cacttatttc cagttaactt
     2401 tctgttctta gctttttact caactggata atatatgcac ttaatgaagt cccaatttcc
     2461 gaatgttgaa gtccatattc aatatttgtc ttcatatatc ctaacttatc gcctacgtcg
     2521 tatctttttc ctttaaactc tctcgcatag accgcctctg tctggttaag agtctgaagt
     2581 gcatccgtta gctgtatctc gtttcctgct cctggttctt gagtttctaa tatctcaaat
     2641 attttaggag tcagtagata acgtccgata atggctaaat tacttggtgc ttttcccatt
     2701 tcaggttttt ctacaaaccc ttttactctg caacgaccct tttcgctatc tgttttagga
     2761 tcaataattc cgtatttaga tgtttcattt tctggtacac gcatgacggc aacagcggaa
     2821 gcttgtgttt cttcataatc catcatcaat tgttgagtca atgggatatc atctttcatc
     2881 aaatcatccc ccagcatgac gacaaacggt tcatttccta taaatgcttt tgcttgaagc
     2941 acggcatccc ccagtcctct tggatgagat tgacggatga agtgaagatt gacttgagtc
     3001 gtttcttcaa ctaaagcgag taattctgtt ttttcctttt cccgcaaatt gttttccaat
     3061 tcgatatttg catcaaagtg atcttcaatg ggtctttttt cttttcccgt tacgatcaag
     3121 atgtcttcga ttcccgaatc caatgcttct tccacaataa actggatcgt tggtttatcc
     3181 acgattggaa gcatttcttt tgccatggct tttgttgccg gcaaaaaacg tgttcctagc
     3241 ccagcggcag gaatgactgc ttttttgacc ttcacttgct gttcctccta aacgacgatt
     3301 aactattgat catctattta acgaagagaa aattcattct ccatttttcc gtcacgtaac
     3361 atgatttctt ttgctgcttt tttgatatct tctccattat acaaaacatt atagattgtt
     3421 tgagtaattg gcatctcaac acctagctgt tgagctagct ctactgctgc ttttgtcgtt
     3481 gccacaccct caacgaccat tcccatgttt tctagaactt cttccagctt atgtccctgt
     3541 cctaacaagt taccggcacg ccaattgcga gaatgaacac ttgtacaagt aacgatcaag
     3601 tcccccacac cactcaaacc gataaatgtc agaggattgg cacccatcgc gacaccaaga
     3661 cggctgattt ctgccaatcc tcgagtcatg attgctgctt ttgcatcatc tccgaatcct
     3721 aatccatgga tagcgccggc accaattgca atgatatttt tcagtgctgc accagtttct
     3781 acgccaatca catcattatt tgtataaatc ctgaaataat cattcatgaa cagtttttgt
     3841 acataggctg ctgctttttg atcaatactt gcagctgtaa ttgttgtgat atcatgtact
     3901 gccacttcct cagcatggct aggaccagat aatacaacaa ttgcctgtct tttttcttct
     3961 ggaatttctt cagccaaaac ttctgagatt cgtttatgcg tatcttgttc tagtccttta
     4021 cttgcatgta taatgattgg cttagtattc attttaccaa taagttcctg tgcgactgaa
     4081 cgaatcgctt ttgttggaac aacaaataaa atagcatctg cgttatccac tgcttctgcc
     4141 aattctttat atccgataat cgattctggg atcgtcaaat caggtaaaaa atgctgattt
     4201 gtatgatact tattgatttc atcaatctgt tcaggtacat ttccccaaat acggacatca
     4261 tgcccgtttt ctgccaatgt ttgtgctaaa gcagtgcccc aagatccagg accaagaact
     4321 gcgatttttt gtttcatgga tattcctcca aataaataat tgataatttc cattatagta
     4381 tacatttctt aaagttggga ctaatttcca ttgtttttaa gtgtttgttt acttctttta
     4441 tcgtttttac ttgaaataat caatgatatg ttacaatagt ctacatttta tttttacact
     4501 cgttatgctt tgcttgttcc taagaggaag cagttttgac aatgttacta ccagcattac
     4561 tattaggagg ttaggaaact tctcctagtg agtattgatt taaaatacat aagagacaga
     4621 ttgaacattt ctaaatctgt caggtcctgc aacggacaga ttgtctctgt tgacggttga
     4681 cataggaact tttgttttag tcaacattct ggatacaaaa cgctatgtaa actggcgcaa
     4741 ctcgttgtac ttggactttt actacggatc catgcaacaa ctaaatagta ctattgcata
     4801 acaaaaaatg gttggcaccc acatgtccca ttaaacagat tataagttga actgtagata
     4861 atgcaaaaaa tgtgttttat aacgtaattg atgccaatat tttaaaaaaa taaagggagc
     4921 agttatcgct gctctcttta ttttttatat tctttttatc cagtctatcc taataatcaa
     4981 actgacgata gtagcggcgt atctctaatg gatggcagtt caactgctcc acatgagcat
     5041 cgatgcgttg tgtcactaca tgcatcaata agaaagacaa aacacgtccg cgatattttt
     5101 cactgatacc ttctactgga tagtcttttg tgtctatcaa tgtataattg ctgcaaattt
     5161 ttggcaaaag tttagccaca cgttcagata acactctctg ttcatcttcg cctaaaaata
     5221 atgtgactgg ggttgtttct tctacgatct ctaacgtccc gtgaagaaac tcggcagcat
     5281 ggatagattt cgatggaagc cagctttgtt cttcccagta acacatggca tatgaataaa
     5341 cagctcccca ttgattccct gctccaataa aataatgcat actatcacgt cgatgttttt
     5401 ctgcaaatga taagccaaat gcatctgctt ttttctctgc ttccactaac cctgttggta
     5461 aataacgttc gagctgttca tagtattctg aataagcttc gaattctcca ttcaaataca
     5521 tcaatcgatc tgctgccata aaaaatttga tttgttctgt tccaggagct ggataagtga
     5581 ccacaaaatc gcatagctga gacaacttac tgttcgcttt atcgataaat ccaatcagtg
     5641 tcgctcctac tttcttgatt tgttctactg ctttcactac ttcttgagtt gtaccggtaa
     5701 cagaggacaa aataacgata gaatcttttg ttaatctttt gtttccagta gtgtaatatt
     5761 ctgcagcatg ctgtacaaag acaggcaagt tgcttttccc attcatataa gtgacagctt
     5821 gcatcgagga agcatacgtt ccaccgattc ctagataata gacagcgtca aatttttccg
     5881 cataaagttt atcgatgatt tcttctactt gcggacgtaa agctagtgcg ccttttattg
     5941 ctttgatttg ttcttcttca ttgaatttca gcatgatttc cacctcttat aatttacttt
     6001 tcaagactgt atcaaagtct tcaaaaatct ttttgttcaa gtcaatacct ttagctactg
     6061 cggttttata agcgaatagt tgaaacggaa cgacaagcgc taatacagac agatattcat
     6121 cacattctat tgctaatcca agtgttttct cgtttcttgc tttttttgtg gtaatggtga
     6181 gagtctctcc aacgtaccga ctcatatatc tttgcaactt ttgcgaacgt tccttattta
     6241 cactttcccc ttcaataaag aatagcaaat gcgaagcatc tgcctccagg tatgggccgt
     6301 gcatataagc ttctaactca aatccttgtg acggaacgcg gattgtttca gtcaatttcg
     6361 tctctgcttc tttagctgtt ccccagtttg gaccatatcc aatggtgacg aaacgctgtg
     6421 ctaaacgaaa taaactttga tatccatcaa aaaattcttc cgttttttga ataatcgctg
     6481 gtaaatggtt gattattttg cgcaactgtt gcatgtagtc ttgttctatt tttttgctga
     6541 ttttattttt acttattcca attcctatcc ctaagagtag taactgtaaa acagtcgcag
     6601 aatagccttt tgtaacgaag cctactgatt ctaccccggc acctaaatca ataatttgtg
     6661 cagcttctaa agcaagtgga ctttgcacgt catttgtgat agcaacaaca ggtaaccctt
     6721 gtttttgtat catttttact gcttcaacag tagaagcact tttcccgctt tgtgacacag
     6781 caatcacagt atcgattgaa ggagaaagtt ttccataatt gaaaaaataa aaaggttctt
     6841 gaatatcgac agtaagatca ccataagatt ccaatgctgg cttcgctgct aaacaagcgt
     6901 tataagatga acccgttgct aagatcagta aattcactgt ttttttcata tgttctcttg
     6961 tcgaaaaatc attctttttc aagatcgtac ttagtacttc cttttcttca tagatataat
     7021 cttgcatcgt aaacattttc tctgctcctt ttttaaaaaa tccctatcca cgaaccaaaa
     7081 attccaaaga aagctagtcc gatcaagatc gttaatggtt tcacttcttt cttcaataga
     7141 taaaacacta tgccaaatgc tccaagacat aagatatttg gaacgatatc atcaaaaata
     7201 ctttgaacag tcacagcatt ttctcctgaa ccgattttta aaggaatatt gatggtgatc
     7261 atcgtggcag tcattgctcc aacgaccatc agtccaataa tagacgcgcc taatgtcaaa
     7321 ctttccatca taccgtttgc ctggatcttt ttcaaaaaac ccgttcctaa gcgatatccc
     7381 caacctgttg ctagatagcg aaacagaatg tgcggaatat tgaaaatcaa aatgaacaag
     7441 atagggccta aaatatttcc ttgcatggct aaagaggtac ccacacctgt ggcaatcaat
     7501 cgcaaagttc cccaaaagaa agaatctcct aaccctgcaa gaggtcccat caaagatgtt
     7561 ttgatactat cgatcgcagt cacatcaaat tcaggatcat ccttattttc ttcctccatt
     7621 gcagccgtga ttcctaaaat cagtgtaacg atatgtgggg tcgtgttaaa aaactctaga
     7681 tgccgtttca aagctgctgc catttcttct ttcgactgat ataacttttt caaaatcgga
     7741 atcattgcat aaacataagc catattcatc tgacgttcat aattccaaga aaactccatt
     7801 tgaaaagaac gccaaaatac gtgatttagc tcttttttcg ttattttgct gtttttgatc
     7861 tcagaattct tcatcttctt ctacctcccc tgcagttgtt tgaagtggta gttgacggac
     7921 attcataata ttgactacca caactgctaa aatcgcacca aaaatagcta ctccggtcac
     7981 aggaatctct aagtaagaag ctatagcaaa acctaagaaa aaatatggtg caacttgttt
     8041 gttgatcagc aaacgagcaa gcatcgcaaa accaagagct ggaatcaacc ctgtagctac
     8101 tgacaacccc ttttgaacga agtcaggaat catatttaat agatttccga tcgtattgct
     8161 tcccaccata taagaaacca ttaccacaac accaagcatc aaggaaagac cgataccggc
     8221 aagcaagtgc atatgctcga ctcctttata atttccttct tctgcataat tgtctgcttt
     8281 atggttcata attggaatca aaagccctaa gtaaatattt ttcaatatca atgtcaacgt
     8341 agcaatcggc aaacctagta aaagcgcggt ttctgtaccg gctccagctg taatagcaaa
     8401 agccgtccct aaaatcccac cggtcacaac atcaggtgga atcgaagcac ctactgagaa
     8461 cgatccgata aatgctaact ctaaagttgc gcccataatg atcccagttt ttacatcacc
     8521 taacacgatt cctgtaaaca gccccgtcac aatcggacga gaaagcaacg atgtccctag
     8581 tgcatattct gattgtgcga caaatgcaac aattcctaat aagactgctt gtacaatcat
     8641 tgaggttctt cctttcttac aaaacttctc ttacatccac ttttttatct gctggtactt
     8701 gacggatttc tacttctatt tgtttgtcaa ttaatcgttt caagcgttcc tcctcttctg
     8761 gtaaaatatt taccgctttt ccgatgctgc gagtaccagg ttttgccttg atccctccta
     8821 agttgatggt tttgatttct ggatacgctt ctgccagctt atatgcatcc tcgatcgatt
     8881 ccactacgat gaataactta tatttatctg tcaccccgct tttcaacgcg gcaatcgagt
     8941 catcaattga tttgatcact agtttgactc cttgtggctt ggctaatttg atcgttgtct
     9001 ttcgtatctc attcgtaggt acatcatcat tagcaatcaa aatacagtct gcgcctattg
     9061 tttgtgtcca tgaaaaagct acttgtccgt gtaataaccg atggtcgact cttgtcaata
     9121 caatcatttt tcttcctcct aaaattcgtc ttcttctatt ttctgattga gccgctcgtt
     9181 acaaaactgg atcatttttt ttgaatcagt tagcacctgc tggatcaaaa cagttgtctc
     9241 tacttcagcg tccaaccgag atactagctc aatcaatagt ggcaaattca atccggcaac
     9301 gagatagaca tgatcccgat tgatatattg taagaattcg ttgttgacac ttccgccaaa
     9361 aagatccgtc agaacaatga tttctttatc tttgtacctc ataaaaagct gccttactgt
     9421 ttccgctaaa tcaaaattct catcagtata gcaatcgaat acagtaatag aatgattttt
     9481 cccatagatc agctctaatg aatctaatat ccctgaagca aattttccat gggaagctaa
     9541 gataatttct ctatccactt tttcatctcc tcttttcact tacacatact aaaaaagcaa
     9601 gttgcatgcc aaaattagtg gcaacaactt gcttatgttc aaaattcttc ttctatcgag
     9661 agaaactctg tatcttcact caaaatatca tagatatagg caatctcaga attggggatc
     9721 atgacactat aatgctcttc aatgacacta aacgcagatt taatagcaga taacccttct
     9781 ttttgacact gcttccattt ctctaatcct tgataggttt cgattggcat ttgtcggatc
     9841 aatcgttcaa tgagacagct cacatggaca tacaacgcta attttttggg attgctgatg
     9901 cttcgattca actgcacctc taattctctc ataaaatgat cgatttctcc catcaccttc
     9961 tctgtatcta gaatagtcac ggaatccagt actttttcca atgaaaaatt acgaatgatg
    10021 cgctgattga atatttcctt ctctgaagca ttcatagtag tgtccaacca ttcagctagt
    10081 acagacatct tttcccccga gatgaggtct tctaatgaaa gatagtcgat attggtgata
    10141 tcaggatctg tggtgccaat cattcctacg acttcgtata tggaaaataa tgggtcactg
    10201 tttttcttat cttttaattg ctgatattca tatgggatga ttttcaactg acaagccgta
    10261 ggtaagcttt tttctagcag ttcacttaag tggaccgccg taccgattcc agtgaagcaa
    10321 gtagtaatga gtgctttggg tttgttttcc tctggataga gcagtcgcca ttctggctgg
    10381 atctgtcgaa cagtctcttc tgctatctct tctaatgata catttttttg caggcattcc
    10441 cctactgtga ttgctaaagg tgtcgtcaca ttattcaaaa tcaaaagcgg tacggataac
    10501 tgttttttaa aaaactgatg gatttctttc agagatccca tgtctactaa tatgatcaat
    10561 ccatttgaaa catcgctgtt ttcgcaataa tcaaggattt ctgaagcaat ctgctgtggt
    10621 gtgacatcta gtggcatatc aaatgattca aatagatcct cttgtaataa acgatttact
    10681 acatttgcca tactgctagc agttgcataa ccgtgagcag caatcatcgc ttttggcagt
    10741 cgtttttctt ttgaccattt cgcttttttt agatagatcg tcagtaaaat ccgatccata
    10801 cttgtgtctt caatatctaa tttgatctta cttaattcca gaattcgttg gacatagtga
    10861 taactagatg gataagactg agaaacttgt tgttctaatt ctttgattag ttggataata
    10921 tcatgttctt ccggtaacca tttgatagtg cttcgctgat agagatagta actcaaggca
    10981 tagacactgt ttccatcgaa tttgatttga taggcagttt ccatttgtcg aaaggtctct
    11041 cggatagatt gtgtccaata aaccagcagc tcgtgttttt cttgtctact ggtctcaaac
    11101 agcaaaaaat caaataattg aatcacttct tgctttaatt ttccttcgca agaatatagc
    11161 agacagttat ttttcttgaa atcaattagg atttgctcaa aacttttaac gattcgctgc
    11221 tgttcgggct gttgcttctc cattaattgc tctaaggtct ggtcttgcgt aatatagatt
    11281 ccttgctgtg ctgtattttt agtaataggc tgcgcaagta gttctttttg caaatgatgg
    11341 atcgtgacat ggatttcagg ctgttcacgc tgttcagcat atgcttttgc tgctgaaact
    11401 ttgacgatat ttttcagctc tcctatgttt ccggataatt tctgacttgt taaaagtgac
    11461 aaagctggtc cactgactga caaagggctc cccatttttc tttgttcttc gagaaaaaaa
    11521 gagtaaatca gttctagccg ttcattacga ctacgctgat taagtgcagg tagttcgatt
    11581 cggataggta ttcttcgaat gaaagttgtt aagaaatgac tagtaatctc ttcagtcgta
    11641 gcaaaaaaga gacgtgtttt gatccggcgt gacaaattag gttctcccat acgataaatc
    11701 acaccttgat caagataggt gaacagtttt tcctgacctt ctggtgttaa gcgatgtacc
    11761 tcgtctaaaa acaagatccc accatctgct gcttcaaaag cccctttttt attttcttct
    11821 gcaccagtgt acgctccctt aacatgtcca aacaaattgc tagtcaaaag ttcaggatta
    11881 tctgcatatt gtgcacaatt aaaagtgatg aatggtgcag aatcttctag cagatcatga
    11941 agcagacaat actgatagac caaattaact aaataacttt tacctgtgcc actttcgcct
    12001 gtaatcaaaa gtggtaatcc gccatctgga tagtaaagag ctgttttgat ttgttcgata
    12061 ctttctttca aactggcgtc gtatccgatc ataaaagaga ataagtcttt tggttgcgcg
    12121 aaaaaaggct gttcctcttt gagatcatcg atagaaaaat aaaaattcgt gcgtaactta
    12181 aaaaattgtt tttcaaaagc ttctttgtgg aaataaatca ctggtcgaga attgattttg
    12241 actagtagtt gctcatcatt taactggttc aaataatgac tgactgtgtt tctttttacc
    12301 ccaaaatgat cagctaacct ttgtgctgta aagacctcgt tcatatgttc taaatcgaaa
    12361 aaggctgtct ggttctctaa gtaacataaa atttccttct tcaacaactt attcacccct
    12421 cctccatcat agaaaacgga ttcattttgt caataaaatc tttaataaag aattgaaaaa
    12481 tatatcttat tatagacagc agcctactta tcccttataa tttttattct aaaaaacgta
    12541 cagttccttc cagtttttat actggaattt caacatatga aagaataagg aggctatgac
    12601 tattcaatga aatcggataa atataaaaac aggaaacata cttcctggaa aacttttcga
    12661 gaaatcatcg gatcttatct cgctgtagtt gtatttttgc ctttgcttta tatggctatg
    12721 acagaacaaa ctcgttttat tggcgtagat acgcaatata tagccgaaac aggattttta
    12781 ttcatcttta tcttgatttt tgcgttctat aaatccttcc atacaaaata agattcacag
    12841 atttcaactt tcctgtctgt ttttagctat tacaaatcct tatatgtggt ataacaacaa
    12901 tttattggca taatgaggaa aaacgagtta tgatgctttc agagagtaaa gaggtactgt
    12961 tgtcaccatc ctttttattg attacgtaca ccactcttag ttttttccca ttgattacga
    13021 aaacaaggta gaaacattga tgttcctacc ttgttttctt tttttatttt cagatcaaaa
    13081 tctcaagtgg cttttgactt gatgtcggga ggttgtcttc gttccatcaa acctgataga
    13141 atctgcgcca aaccatagtt ttctccacta ttatttattc ctctaagata gtacccacaa
    13201 taaacgaata aatatttttc tcattttcat actccttctt tgaaaatcaa ttagatttct
    13261 ctcaacccac ttttttacaa aaattacgta gcaaaaaaga ttgcaagaac aaattaaaaa
    13321 agaagtaaat agagtttcat ctata
//
LOCUS       DAZAID010000076        13126 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000076 DAZAID010000000
VERSION     DAZAID010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.077"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..667
                     /locus_tag="ACG9BE_002112"
     CDS             <1..667
                     /locus_tag="ACG9BE_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032845.1"
                     /translation="PWFALQNTPLNIIQAPWRFMIFSTLFISFSVSLFISPQLEKFSY
                     TRRRNFILLLILAISILNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYK
                     KDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSK
                     NATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRT
                     SHE"
     gene            complement(934..2103)
                     /locus_tag="ACG9BE_002113"
     CDS             complement(934..2103)
                     /locus_tag="ACG9BE_002113"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN3032846.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            2307..3482
                     /gene="mltG"
                     /locus_tag="ACG9BE_002114"
     CDS             2307..3482
                     /gene="mltG"
                     /locus_tag="ACG9BE_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFN3032847.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            3618..4097
                     /gene="greA"
                     /locus_tag="ACG9BE_002115"
     CDS             3618..4097
                     /gene="greA"
                     /locus_tag="ACG9BE_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFN3032848.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            4266..4997
                     /gene="liaF"
                     /locus_tag="ACG9BE_002116"
     CDS             4266..4997
                     /gene="liaF"
                     /locus_tag="ACG9BE_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFN3032849.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4994..6061
                     /gene="liaS"
                     /locus_tag="ACG9BE_002117"
     CDS             4994..6061
                     /gene="liaS"
                     /locus_tag="ACG9BE_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFN3032850.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            6068..6700
                     /locus_tag="ACG9BE_002118"
     CDS             6068..6700
                     /locus_tag="ACG9BE_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN3032851.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6840..7505
                     /locus_tag="ACG9BE_002119"
     CDS             6840..7505
                     /locus_tag="ACG9BE_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFN3032852.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            7533..7826
                     /locus_tag="ACG9BE_002120"
     CDS             7533..7826
                     /locus_tag="ACG9BE_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFN3032853.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(7894..8841)
                     /locus_tag="ACG9BE_002121"
     CDS             complement(7894..8841)
                     /locus_tag="ACG9BE_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFN3032854.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(9019..9822)
                     /locus_tag="ACG9BE_002122"
     CDS             complement(9019..9822)
                     /locus_tag="ACG9BE_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFN3032855.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9968..10882
                     /locus_tag="ACG9BE_002123"
     CDS             9968..10882
                     /locus_tag="ACG9BE_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN3032856.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10930..11400
                     /locus_tag="ACG9BE_002124"
     CDS             10930..11400
                     /locus_tag="ACG9BE_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFN3032857.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            11485..12093
                     /locus_tag="ACG9BE_002125"
     CDS             11485..12093
                     /locus_tag="ACG9BE_002125"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFN3032858.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            12227..12427
                     /locus_tag="ACG9BE_002126"
     CDS             12227..12427
                     /locus_tag="ACG9BE_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3032859.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         4444 a         1992 c         2585 g         4105 t
ORIGIN      
        1 tccatggttt gccttacaaa atactccttt aaatattatt caagctcctt ggcgttttat
       61 gatattttca acattgttca tatctttttc agtatctcta tttatttctc cacagttgga
      121 aaaattttct tatacaagga gaagaaattt tatactatta ttaatattag ctatttcaat
      181 tttaaattat tcatcagtag ttaacatggt gaaagaaatg aattttcaag gaacttattc
      241 taatgaacaa atggaaaata aacttgaaga atttgagaat tttgattatg tacctatagg
      301 ctataaaaaa gatcaattgc ttttgaaaaa ccatcaagtt acagaagatg gtcaagttat
      361 ttatagtagc ttttctccaa ataaagatga aattacattt acgtttaaat cttcaaaagg
      421 caaaacaata gttttacctg tattccggta taaaggatta acggttactg ttaattcaaa
      481 aaatgctaca gtactaaaac aaggaggtcc ttttgttaca gtaaaaagtg aaagtggaat
      541 aaataaagta acaatcaggt attcatacac atttttttct aaaatctcat tatttatttc
      601 tataatcacg ctaattgttc ttctttttta ttgcataaaa ttaaaaataa gaacaagcca
      661 tgaatgattt gctagcacat attttagcaa tctaaagata ctattttaga tttaataggc
      721 cctttgtcaa ataatgttgg taaagagaaa tcttagaata atttggacag aacagaaaga
      781 agaacacctc tttctgttct gtttatttgg aattaaagtc tgtagcaaaa attgattttt
      841 agatttgtcc cacactcttt cctacgttta gtcgagtact ttttttaagc aaatgctatt
      901 caaagaagta gtatttgcta tcttttcatc atatcagcta tttgccaact tagtagttcc
      961 ttccatcagg tcatagattt ggtttcttag atcagcagta ggcagtttga tagttttgtc
     1021 agcttttata ttgctttcca tgatgatagc atcctgtgaa tctttgctaa atatagcaga
     1081 tgtatcaaaa gagtcgatat ttccttgcga caggatataa ttatctccgc tatatacccc
     1141 accactatat ggatgtccat tcgcattttc acgccataat gagaagagta agtctttgga
     1201 aactagtttt ccagataaaa cttgtgtaaa cagcgtattt aaatcataga cggacatact
     1261 gatattacca gtccctaatt cgttatggat ctctgtttca gattctgtca gtggtttaaa
     1321 gtaatcatcc tctttagaca tttgatagga tagtgcatga ttggatgaat ttttgacttg
     1381 gtcataaaat tttgtatgct gtaatcctaa aggttcgatg atatcatcgg tcaataattt
     1441 ttcatacggt tgatttgtta atttgatcaa aattccagtc aatatggtaa agttcaatga
     1501 atcataatga gaggtctgat aatctgtaaa ttttaagtgc tgaatcgcaa attgaacgac
     1561 ttcttcatca gtcatcggta tggttggaga agcagttcgg tgcaagcctg agcgcatata
     1621 cagcaagtca cggatagtaa tttgctggct gccagcaatt tgtggataga attgagccag
     1681 cttagtatct aaagaaaggt gcccacgctc gacttgtttt aaaatcaaaa gagtagtcaa
     1741 tgctttttga accgaaccaa tctgataata aagagaagga tcatttggtt gttgcgtgag
     1801 tttattcgca tagccatagc ctttttccat gatgatgcga ttatttttct ttacgtaaat
     1861 tgagccaata taatcatttt ttcttaatag ttgatcgatt ttttcaacga gctgttgttc
     1921 attggtagtc agtatcaact ttttttgagc tgattgagca tgggcaacta tctgagagtg
     1981 ttcattattg taaaattgcc atccaagaat agttcctgta gagaacgcac taaggctaaa
     2041 caacacgaca acgatcatta aaaatgaata ttttcttttt ttagcatgtt ttcctttttt
     2101 cataatagtt tatttagtct cctttctttt ttacagaaag aataaagcta aaatagcttg
     2161 tatcttgata acatttattt taatcttata gtaatggatt tactacttac atttttgata
     2221 gaatatttaa ataaaatcat tcacgccaat tcaaatatca tgtataatga ataaagttac
     2281 gaacttgata gtaaggagga gttgttttgt caggaaacaa acaacaccca agtaaagata
     2341 aaaaagaagc cagtaaggca gctacagcag atataatcga caagcggaga aatctgcgta
     2401 aaaaagaaga taagattgtg aggaagatca ttttagtgat tgctcttaca ttattgatta
     2461 ttggcggatt tcttggattt accgtgtatc gttatgtaga tagcgggttg aagcctttag
     2521 ataaatcaga tgaccaatta gtccaagtcg agatcccgag tggctcttct aacaagcaga
     2581 ttggtgagat tttggagaag gacaatatta ttaaaagcgg gattgttttc aattattata
     2641 caaaatttaa aaatttaact ggctttcaag ctggctatta tcagttagcg cctaatatga
     2701 ctcttgacga gattggcaaa cagttgcaag aaggcggtac ttctgagcca acaaaagttg
     2761 cagatgggaa aattgccatt ccagaagggt acgatatcga tcagattgct gaacgggtag
     2821 cgaaggtaac tggtaaagac aagaaagaat ttttagattt agtgaatgat gagactttct
     2881 ttaacgagtt gcaccaaaaa ttcccagaac tgttggaaag tgcatctaaa gctgagaaca
     2941 caaaataccg tttggaaggt tacttattcc cagccacata tgattattac acgagtactt
     3001 cgcttaagga tctagtgatt gaaatggtga acaagacgaa tacggtcatg caaaactatt
     3061 attcagctat caagcagaaa aatttgactg tacaagaagt tttaactcta gcttctctag
     3121 ttgaaaaaga aggcgtaaaa gaaaatgatc gtaaaaacat tgcacaagtc ttctttaacc
     3181 gaatcaaagc aaatatgcca cttcagtcag atatctctgt tttatacgca ttaggtgaac
     3241 acaaagaatt agtgacgtat gaagatacag ctgtagattc accatataat ttgtatacca
     3301 ataccggcta tggacctgga ccttttgaca atccaagcga agaggcaatc aaggcagtat
     3361 tagaaccagc agaaaatgac tattattatt tcgtagcaga tacgagcact ggaaatgttt
     3421 atttcgctaa aacttatgaa gaacatatgg aattagtgga aaaatatgta aataattctt
     3481 agaaaaacag gctctagaca ttgatcattt ttagaaaaac atttacattt gaagaaaagc
     3541 gtggtaatct tttttggact atattcctca aaagattacc ttttgcggta ttttatcgaa
     3601 aaaaggagac gttacacatg gtagagaaag tttatccaat gactctagaa ggtaaagaaa
     3661 aattagaaaa agaattagaa gaactaaaaa cagtcaaacg cggagaaatc attgaacgaa
     3721 tcaaaattgc ccgcggtttc ggagatcttt ccgaaaactc tgaatatgaa tcagcgaaag
     3781 atgaacaagc atttgttgaa ggaagaatca caacaattga aaatatgatt cgttttgctc
     3841 aaatcatcga taacgacggt gtagatgcag atgaggtatc aattggtaaa acagttacat
     3901 tcatcgaact accagatgga gaagaagaag agtacacgat tgtcggaagc gctgaagcag
     3961 atccgttttc tggcaaaatt tccaatgatt ctcctattgc gcaagcattg attggcaaac
     4021 gtaaaaatga tgaagtaact attgctactc caggtggcga tatgttagtt aaaatcgtca
     4081 aagtagaaca agcgtagtct gactgaaaga tctagagagt tcatcttact gatgatacac
     4141 tctagatctt tttctctttg tgtattttag atttcaacaa acgatcttac atctaaagtc
     4201 ctatagccaa cagttaaaaa aataggtatg atgaaatgag aatgtgtcag gagggagaaa
     4261 gagcgatgaa tagttcttgg cgtttttttg tggtagttga agcactactg ctgttatttg
     4321 ctgtttggca aatcgtaaac aatactggac ttttgctttt agtgatattc ggtattttca
     4381 atatatatct agctatgcgc aaacatcctc gtactaaatt tcaaaatttt caattgatat
     4441 taggaagttt ggtcattttt tttagcttag tcaacagtcc tgctttatgg atgatggctg
     4501 tatttgccgt tttatttatt ggtctgaaag gtgtagagat ttcagggata gatttaacca
     4561 aaaatgcttt ttggcgaaag aaacagatca tgatgatcca aacagagcaa ttaaaaacac
     4621 ataataatga gcggaaaaga cagcaattat tcggtaacca acgtattgga aacgacgtct
     4681 atgaatggga cgatatcaac atcgccgtca tttctggtga tactatcata gatttgggca
     4741 atacgctttt gccaaaagat gataatattg tgattgtaag aaaagggata ggcagaacac
     4801 ggatcttagt acctttgggc gtagccatcc gattagagca tgcgacatta gtaggaaatg
     4861 tcttgtttga agaagagcaa ttttctttga aaaatgaaca aatcaaaatc tacagcagtg
     4921 attatgatga aaaccctcgt agattgaaga ttattactaa taccttgatt ggagatgtcg
     4981 aggtgatacg aatatgatgg gaaaaatatc cagagcgatg ctagctgttt attcggggat
     5041 tgcggctttc cttattattc tattttcact tttcacttat ttttatgcca gcaatcaaag
     5101 tcattggtgg ggagaattac tgcgtgcacg tttattatat gttccgctta tcttccattt
     5161 gttagccatt tccttaggtg taggattaat tgtctttcta ttattatcac ttattcaaaa
     5221 gacaaaatac gggaaaatcg aagaaaagct gcgtgcactt tcttctggca attatgaatc
     5281 caaattgttg cttcttccga tcccaagtgc atcggacgat ttatacatca aagatattga
     5341 taaggaaatc actaagataa aagaaaaaat gattgaagta tctagtgaat tacaaattgt
     5401 aacgagccgt ccccaatatg tagatggaca aaccaaagaa gaaattttag aattagaaag
     5461 acatcgatta gcccgtgagc tgcatgattc agtttcgcaa caattgtttg cagcaatgat
     5521 gatgatgtcg gcattgacgg agcaagcaga aaaaagcgag acaccagaaa tgtttcgcaa
     5581 gcagttgaaa atggtagcag agatcatcaa cgcttcccag tctgagatgc gtgcgctgct
     5641 tcttcatcta cgtccagtca atttagaaga gaaaagttta aagcaaggta tcgagcagtt
     5701 attgaaggaa ttgcaaaata aaatccagat ttcgctgaaa tgggatgtag aagatgtaaa
     5761 actatctagt tccattgagg atcacttgtt ccgaatcgtt caagaattgt tatcaaatac
     5821 attaagacat gctaaagcca atgaattaga ggtatatttg cacaaaatag acaataatct
     5881 tttgttacgt attattgacg acggaacagg gtttaatatg aatgaaacga aaacgggaag
     5941 ttatggattg aacaatatca aagaacgagt agctggtatt ggtggaacag taaaaatcat
     6001 tagttttaaa ggtcaaggta caagtgtaga aatcaaggtc cctttgatga aggaggcata
     6061 gataaggatg ataaaagttt tattagtaga tgaccatgaa atggtgcgct taggcgtctc
     6121 tttttattta tctattcaag aagacattga agtaattgga gaagctgaaa atggacgaca
     6181 aggctatgag aaggcgatga cacttcgacc ggacgtcatt ttaatggact tagtcatgga
     6241 agaaatggat ggtatcgaat caacaaaagc aatcttaaaa gattggccag aagctaaaat
     6301 cattatcgta accagtttta ttgatgacga aaaagtttat cctgccattg aagcaggtgc
     6361 agcagggtat ttgttgaaga cctctaccgc acatgaaatt gctgatgcta taagggcaac
     6421 gcaacgtggc gagcgagtat tagaaccaga agtgacaaca aaaatgatgg aaaaaatgag
     6481 tcggcgaaat gagcctgtat tacatgaaga actaacgaat cgggaaaatg aaattttaat
     6541 gctgatttct gaaggaaaaa gtaatcagga aatagcggac gaattgttta ttacactgaa
     6601 aacagtaaaa acacatgttt ccaatatatt ggctaagcta gaagtggaag accgtacaca
     6661 agccgctatc tatgcattca aacatggttt ggtgaaataa aaacgatgat aaagaggata
     6721 atcaaaagct gagaaaataa ctattatttt tcttggcttt tttacaataa tgatgcttgc
     6781 ttttgttttt gtaaaggctt tgttatactg agaaaagaag atactaaagg aggaaaacgg
     6841 tgaaacaaaa ttttgcaatt gtaggtcttg gacgatttgg cggaagtatc tgccgaacac
     6901 tgatcgaagc tggtcaagaa gtactggcta ttgatagtaa tgaagatcgg gtcaatgaat
     6961 atatgaatat agccactcat gctgttgtgg caaatgcaca ggatgaaatg acacttcgat
     7021 cattaggcat aagaaatttc gatcatgtaa ttgtcgctat tggagaagat attcaagcca
     7081 gtatcttagt tactctgatg gtaaaggaaa tgggcgtacc aaatatttta gcaaaagccc
     7141 aaaatgagta tcatgccaga gtacttgaaa agatcggagc tgatcgcgtc gtccatcccg
     7201 agcgagacat gggaataagg attgcgcata atttagtgtc aaagaatatt ttagattacc
     7261 tagagttgtc agataaatat tctttagcag aaatccgagt ctccaatcct aaatttttca
     7321 acaaaacatt agctgaatta aatttcaggc aacgttttga cttaacagta gtcgccatac
     7381 gaagagtaga tcaaacggtt atcgcttctc ctgcagcaga tgaatatgtt cgtgaaaatg
     7441 ataatctact ggttattgga gagacaggcg atgtggatat tctagacgat aagatgaatc
     7501 aatagaaaga aaagagaaag taggttgttg ggatgaaatt aacggtaacg ccaaaagcac
     7561 aaaagtggtt tgactcagaa attgatctgc cagaaactta cggaatacgt ttttttggaa
     7621 aagtctacgg caaaacagag gttcatgatg gtttttcgat tggaatgtca gttgaacagc
     7681 cagaacatcc tgtaaagaaa gtggaaattg atgggacact tttttttatc gaggaagcag
     7741 atgaatggtt ctttaaagga tatgatttac ttgttgatta taatcccaac ctggaggagc
     7801 caacctatca ctttaaaaaa caataaaaaa agagagggtg acacaatttt tgtcaccttc
     7861 tcttttttct tttgaaaata ttgttttcag ctattaatat cctgcagaca attttacttg
     7921 attggtttga agctcatggg tggcaaagta gcttttcaga ttggttaaaa aaatagccat
     7981 taatttcttt tgaaaatcag gtgtcaaacc tgcaatgtgg gaagtaatca gtacattgtc
     8041 cattttccaa agaggatgat cttgtggaag aggctcttcc tcaaaaacgt ctaatgctgc
     8101 aaaggaaagt tgctggttat ttaaagcctg caccagatct tgtgtcttaa cagaagcacc
     8161 acggccaaca ttgatgaata cagattttgg atcaaagtgt tcaaacatat gctgatcaaa
     8221 gagcccagtt gtttcctccg ttagaggtaa gatattgact acgaaatcta cttcaggaag
     8281 aacttccttt agttgattta aagaaaaagt cttttccatt ccttctgctg gatgaccgga
     8341 tgtattgatt ccatagcagt tcatgtctaa tgctttgcat ttttttgcaa tcatttttcc
     8401 aatatggcca gttccaatga ttagtatttt tttcccttgg atagattggt aatggaccga
     8461 tgaacctaac catttcatgt cactctgtgc cttttgtgct tgaaaaagac cacgagaata
     8521 cccaaggatg attcctaaga catgctccgt aatcgattca ctgtggatgc cactaccatt
     8581 tgaaagtaaa atatgtttct ttgaaaaagt ctcaaggggt agctgatcaa caccagctga
     8641 gatagattgg acccatttca aggtagaaga ggctaatagt ttttcttggt agtctgagtt
     8701 ccagcccaaa ctgatctcta cattttgtag atcctctggt tgtaattctg ttttaaagat
     8761 ataaccaggc gctatagtat ggatttcttc gataaattca tcacgaaatg gacgatctgc
     8821 ataaatgatt ctttctgaca tagttaattt cccgcctttc tttactctaa gtgtagcgca
     8881 tacaagaaga actttgaagc gccttgcttg ttttttgaga aagaaatatt tttcaaataa
     8941 aaaaggggct gtgacaaaaa aatgtcacaa cctctaaaag ggtctattga agtagaaata
     9001 acgagttgtc tttatttctt aattgatcga tatttttgcc catcctttga cagcttgagc
     9061 aaaaataaag attcctgcaa aagcaatgaa ggcgctagtg tcaaaagaag gaagaaagac
     9121 catcaagatt gttgcaatta tagtaggcaa tgaaattgaa tagatcaatg tttttaaaca
     9181 atcaagaaaa gtagcttttg ttagtcgaaa gcgtgcatag agaaaagcag caatgttggt
     9241 aaatagcaaa gtgatcagca gattcagaaa actaggataa atagaaaata taaatgtcag
     9301 gacttttatc caaagtggta tagatgcttc acttaaaaaa gaacggagct gcttacctgt
     9361 taagtttttt aaagattcat tggtgtaggg aagttctaat tggttattgt tcaacaaagc
     9421 agaagtcgtg cctgtattag gaagagctat gataagtttg ttttttaaca tcccgacact
     9481 aacaaaattc cccatcaaat cagtagagat gtcctgttct gttcgttttc cctctggatc
     9541 aaatgtgaag ataatggagt ttgtttggta aatgaatcct tctttttctt cggtttgaag
     9601 ctgcccgttt tggattgtaa aatcaggaat gcgagtagca atcttttgcc catccaattg
     9661 aatgttgttt aagacttgaa aaacttgaaa agagattggc aaagccataa ttatactcaa
     9721 aactaaaaga tagacaatgg ctttccaaaa tgggacattt ctcgcctcct ttaggtcttt
     9781 gaatcgtata aaggaactga taattaattg ttttgttgtc ataagtactc ctcatctagt
     9841 agtgtcatca cttgcaattc tagcgaagta tcaagtgaaa tacaagtgct ttaaggaaac
     9901 agctgaatga ttggtgaaat atcaaaaaaa tgttattctt gtctaaagac tatccaatat
     9961 aaataaaatg gaggaaggaa tagtgcctac acagccacag agatcatcaa aaaaactatt
    10021 tggagtagtg gttttgaatg cggtgatcac tttatcagaa tttattggag ggattatgtc
    10081 agggagtttg tctcttgttt cagatgcttt ccataacctt agtgatacat tggcaattat
    10141 tttttcttac ggggcacaaa aaatggcccg aaaagaagca aataaaaaac gtacgtatgg
    10201 ctaccagcgt ttagaaatat tgagtgcttt catcaactca ttcatattga tcatattatc
    10261 cttgttttta accgttgaag cattcaagcg gttcacttca ccagaaaaga taaatagtca
    10321 tttgatgcta acagtagcag ttattggatt gctcgcgaat ttgttctcag tattattatt
    10381 acgacaagaa gcagatgaaa gtttgaatat aaaatctagt tatttgcatt tgttaagtga
    10441 tacactgtct tcggtaagtg tgattatcgg agcaattttg atccgttttt tcgatatcta
    10501 ctggatcgat ccagttatta cactgatcat ttcgatatat atattgatag aagcgataat
    10561 ggtgatcaaa aaagctgccg ctattttgat acaaagtgca cctgctatcg actatgaaaa
    10621 aatggaacaa gaaattgaag caatcgatgg ggtaaaggat gttcatcatg tccatatttg
    10681 gcagtatagc gaaaagataa ttattttcga cggacatatt gattttgaag atcagctact
    10741 aagcgagatt gaaaagatat atcctcgtat aatttcttta ttaaaattaa aatatggtat
    10801 tacacatgtg acgatccaag cagagacaaa ggcaaaggat caaaaaaaat taattttttt
    10861 aaataaagat gaaagttatt gaaatataga gtatgataaa taagaatttt taataaggta
    10921 ggttggctca tgaagcagta tcgacagtta aaaggatatt tagaagcaaa aggatattgg
    10981 gagatactag tctatctctt ttttggtggt ttggctacga ttgtaaattt tgttagtttt
    11041 gctctcgcac gccaatattt tgatttaagt atggccttat ccaattctat ttcgtggttt
    11101 tgttctgtat tgtttgcttt tgtaacgaac aaactttggg tattccattc aaaatcaccg
    11161 aattttacac atgcacttat tgaatgcggt aagttcttct tctaccgtat cctttcttac
    11221 ggattggaca tgggggcaat ggtattgttg atcaatgtta tgaacagcaa tgagtatgtg
    11281 gcgaaaatca tcactcaaat tattgttatc cttgcgaact atattttcag caagttgttt
    11341 atttttaaag aaactgaggt ttttgaagaa gaagtaggtc aaagtgaaaa agacgactag
    11401 gcataacaat tgaagaattt ccttctcttt gttactcttt gtaatgtaga cattattaag
    11461 aaaacattga ggaggaattt ttttatgact tacacattac cagatctacc ttatgcatat
    11521 gacgcattgg aaccttatat tgacgaagaa acgatgcatc tgcatcatga taagcatcac
    11581 aatacttatg tgacgaattt aaattcagca attgagaaat acccagaatt aggcgaaaaa
    11641 acaatagaag aattattatc tgatatggac gctattccaa cagatatcaa gacagctgta
    11701 cgtaacaatg gtggcggaca tgctaaccat tcatttttct gggaaatcat ggcaccaaat
    11761 gctggtggcg aacctacagg agaaataaaa gaagcgatta atgaagcttt tggtgatttt
    11821 tcttctttta aagaagaatt caaaaaagca gccgctggac gatttggttc tggatgggct
    11881 tggcttgtaa tggaaaatgg aaaattagct attacctcta ctgcaaatca agattctcca
    11941 ttgatggaag gaaagacacc aattctaggt ttggacgttt gggaacatgc ttattatttg
    12001 aaatataaaa atgtccgacc agattatatt gcagcattct ggaatgttat taattgggat
    12061 gaagtaaaca aacgtttcga agcagcaaaa taaccatttc ctccgtcata tcagtcttaa
    12121 aaataatttg gaaaataaaa gagcgaaagc ttgctagcgt tttctaaatt gggtataatg
    12181 ttcttgcaag gttacttgca agaacttttt tggaggatat acatttatgg aacacggaac
    12241 ggttaaatgg ttcaacaatg aaaaaggatt tgggtttatt acagtagacg gcggagatga
    12301 cgtatttgtt catttctcag caattcaagg agacggcttc aagagcctag aagaaggtca
    12361 agaagtagaa ttcacgattg ttgaaggtgc acgcggacct caagctgctg aagtgactaa
    12421 attataataa tttgttgaat aaagacacga cttcttagag agtcgtgtct ttttgtctat
    12481 ttatgaagtt tttagatttt ttggctaagt aaatgcattt gttcacttga catgtcctaa
    12541 ctaattatca agcaaggaac attttcttcg ttagtaatgg attttaaaga aatttcagtt
    12601 tttggatgtt gaataaaaga ccatttgtga aaccactagt atacatactc attaccatgt
    12661 caattctatt actttaaaag aaaatcgttt tactttgaag aggagcatat ctatccatta
    12721 aaagatgatg gtaatgcaat cactagttcg agtcatttta agagccatgc agtgagctgg
    12781 aaataaaacg aatagaagat gggtttaaca aaaggaccag tcataggaag aaattgtcgt
    12841 gaaatcgaat gataatctga acggtaggca aagactgtta aaaagatggg aaaggtatgt
    12901 gcgatcattt tactgcacga ttatttggaa aataccttgt tgttcgcttt ttctggaaaa
    12961 aatacataaa aaaagataaa acgattgcga actgcgtgta ttcggtcttt cgatcttttt
    13021 cacggtatgg tatgatagtt aagaattttg agaaatttta aaaaataata atcgttacac
    13081 aaaaatgttt tttaaagttt tcttatgatt aaaaagtgaa tttgct
//
LOCUS       DAZAID010000077        13086 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000077 DAZAID010000000
VERSION     DAZAID010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.078"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACG9BE_002127"
     CDS             complement(79..2199)
                     /locus_tag="ACG9BE_002127"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN3032860.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACG9BE_002128"
     CDS             2429..3607
                     /locus_tag="ACG9BE_002128"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFN3032861.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACG9BE_002129"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACG9BE_002129"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFN3032862.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG9BE_002130"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG9BE_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFN3032863.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACG9BE_002131"
     CDS             complement(4960..6213)
                     /locus_tag="ACG9BE_002131"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN3032864.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG9BE_002132"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG9BE_002132"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFN3032865.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACG9BE_002133"
     CDS             7790..9136
                     /locus_tag="ACG9BE_002133"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFN3032866.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACG9BE_002134"
     CDS             complement(9444..9830)
                     /locus_tag="ACG9BE_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN3032867.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACG9BE_002135"
     CDS             complement(10098..10247)
                     /locus_tag="ACG9BE_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032868.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="ACG9BE_002136"
     CDS             10709..10921
                     /locus_tag="ACG9BE_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032869.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="ACG9BE_002137"
     CDS             complement(11199..12998)
                     /locus_tag="ACG9BE_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFN3032870.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2354 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaac
//
LOCUS       DAZAID010000078        12941 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000078 DAZAID010000000
VERSION     DAZAID010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.079"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..1191)
                     /locus_tag="ACG9BE_002138"
     CDS             complement(43..1191)
                     /locus_tag="ACG9BE_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN3032871.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(1313..1909)
                     /locus_tag="ACG9BE_002139"
     CDS             complement(1313..1909)
                     /locus_tag="ACG9BE_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN3032872.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2109..4751)
                     /gene="alaS"
                     /locus_tag="ACG9BE_002140"
     CDS             complement(2109..4751)
                     /gene="alaS"
                     /locus_tag="ACG9BE_002140"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFN3032873.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5139..5570
                     /locus_tag="ACG9BE_002141"
     CDS             5139..5570
                     /locus_tag="ACG9BE_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3032874.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(5615..6964)
                     /locus_tag="ACG9BE_002142"
     CDS             complement(5615..6964)
                     /locus_tag="ACG9BE_002142"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN3032875.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(7034..8197)
                     /locus_tag="ACG9BE_002143"
     CDS             complement(7034..8197)
                     /locus_tag="ACG9BE_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN3032876.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            8326..8649
                     /locus_tag="ACG9BE_002144"
     CDS             8326..8649
                     /locus_tag="ACG9BE_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032877.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(8832..10181)
                     /locus_tag="ACG9BE_002145"
     CDS             complement(8832..10181)
                     /locus_tag="ACG9BE_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN3032878.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(10250..11203)
                     /locus_tag="ACG9BE_002146"
     CDS             complement(10250..11203)
                     /locus_tag="ACG9BE_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFN3032879.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(11305..12495)
                     /locus_tag="ACG9BE_002147"
     CDS             complement(11305..12495)
                     /locus_tag="ACG9BE_002147"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFN3032880.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(12695..>12941)
                     /locus_tag="ACG9BE_002148"
     CDS             complement(12695..>12941)
                     /locus_tag="ACG9BE_002148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032881.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVIFICPGRVK"
BASE COUNT         3724 a         2717 c         2231 g         4269 t
ORIGIN      
        1 tttaaattaa aaactttaag gagtcttttt atagtttctc atttaatata aaagataatt
       61 gtgatcaaac gtaatttcca attggtaatc tccgattttt tcttctattt gactacgtaa
      121 gattctttct agttctgtat caacaaactg gaatttctca gggaccacta catcaaattg
      181 cagcgttttt ccttgatgaa ttactctaaa atcatgaata ttaagccctg tacctgtttc
      241 aagaagaatt tgctttaact gccgcaagat attatagtat ttttcgttat caacaggcat
      301 tggatccaag tgacagacaa gttccacatc caaagacttt ttaaagtctt tttctatcag
      361 atcaataatc ttatgagcca cattcaaatt cattgattca tctacttcga tgtggactga
      421 tgcaaagcgt ttattgggtc catagttatg caccaataaa tcatgatagc caagtatatc
      481 atcgcaagca tttaaatgct cttccatttc ttcaatctct gctggcgttg ggcgacttcc
      541 taatagttcg tatacaaaat ttcgaagcat cgtcaatcca ctataaagga tatataatgc
      601 taaagcaaaa cctatatatc catcaatacg ccatccagtt acccattcga ttgctgctga
      661 aagcaataca gccagtgtag taaagacatc attataacta tctgttgctg tagctctcaa
      721 tgtttcagaa tcaatggatt gcgcaataac ctgatacatt ttgccttgtg caaatttcaa
      781 aaagatggaa atgattagga caaaaaagac gatgggtgtt aaagagacat tttctggatg
      841 gatgattttt tcagcagaag actctaaaaa ttgaaatcct acataagtaa tgatgatcga
      901 aacaaataac ccactgatat attcgaagcg ttcgtgtcca taaggatgtt cctgatctgc
      961 tggctttgca gcaattttaa atccgatcaa agtcaatatg gatgaagcag tatctgatag
     1021 actgttcact gcatcagcca taatggagac acttcctgaa aaaagcccga tcattagctt
     1081 agagacaaac aagatcagat tcgtaaccaa tcctaagata cccgctagta ctcctttttt
     1141 tgtccgctgt tgcggtattt gtttcttttt ttcttcttgt ttcttagcca agttttttac
     1201 cccttctagt ttgcttgttg ctcaggtaaa aagatggaga ggacgattgt tttcctctcc
     1261 tctcttcctt tttctatcta tcgtgcgtcc tattactatt aaaagatggt cattattctg
     1321 gtttcaacgg tgttgctgca aataacgctt ttgctagtaa tggtgttatg attcccgcac
     1381 ctattgcttc tggtatccca ttgattgctg caacccctgc taaggtttta aataaaagag
     1441 cagagtctac accataggca tctgccgttg cacctgtata aaataatccc atgaatccta
     1501 acactaagac tgtattgacc aatgaaccca gtacccctgc aaaggctgag ctgatcgtta
     1561 ttgatttatt tttttgataa atccagtgga aaaccaagcc tgctactaag ccaacaagta
     1621 ctctgggaac taccgagata atcggattcg tgaacactaa tgtatcaaat ggcgttgttg
     1681 gcatggcata ggcacgaaca attgtagcga ttccccaaaa caagccaatc accatgccat
     1741 cttttgggcc taatactact gcagcaataa ttactgtgat atggataatt gttaaactaa
     1801 tgaatcctaa tggaataaac cccagccaag gaaccatcgc ttgtacgatg atgattgcta
     1861 acaagatcgc acgtaagact agtcgaaagg tcttatttct actattcatt ttttcacccc
     1921 tttttattta tactaatcgt aaaaatttct gtcactttcg tcaactaaag accatccgaa
     1981 tccgaccatt tgattagatg cgccatccaa ttgaaaaaag tatgagggca agacttgttt
     2041 gtcttcgccc ccataatcat gtttcgttca atgtttgaac ttttgttttt ctgtcaaacg
     2101 taaacgccct attgttgcgc aacccattct ttcgcagctt taagtgcatc tggaatacca
     2161 gcaggatttt tcccgccagc ttgtgccatg tctggacgtc caccaccgcc accgcctact
     2221 ttcggtgcga ttgctttaat caagtcgcca gcttttagtc cagcttcatt tgattctttc
     2281 gtcattgccg ctagcagact gacttttcct tcttgttcgt ttgctaaaac aagaatatct
     2341 gaaatatttt tttgtttcca ttggtcagct aattgacgca gctgattcat atctttcact
     2401 tttacttgtg ctgcaatgat tgtttttcca ttgatcgttt ctggatcttt gaatacatca
     2461 ccagcttgtt gattcgctaa cttgctagct aattgttcat tttccttttg tagctctctt
     2521 acttgctgtt gcaattgttc cgcttttgaa acaacttctt tcaattgagg tgatttaaca
     2581 attgttgcta cttgttttag ttgtttttct tcttcttcaa gtagttcgta cgcttcttta
     2641 cttgttacag cctcgattcg tcgcacgcct gcaccaattc ctgattcaga aacgattttg
     2701 aagattccga tgttttctgt atttgctaca tgtgtgcctc cgcacagttc gattgaccag
     2761 ccgccaatat ttactactcg aacgtcatgt ccgtattttt caccaaacaa agccattgca
     2821 cccatgtttt tggctgtatc gatatctgtt tcaattgttt caactgggat agcttcccag
     2881 attttttcgt tgacgattct ttccatttca gctaattcat ctgaagtgat ttgaccaaag
     2941 tgggtaaagt cgaatcgtaa atgtccagga gcgactaaag aaccagcttg atttgcatgc
     3001 tcgccaagta cttctttcaa tgccttgtgc agtagatggg ttgctgtatg gtttttgatg
     3061 atccgattgc gcatcgattg atcgatcaat agatgatagg tttgtccttc actcattggt
     3121 gcttttactt ctacggtgtg cataaattga ccattaggcg ctttttgaac atctgtaaca
     3181 actgctacga tttcgcctac ttcattcatg atccatcctt gatccgctac ttgcccaccc
     3241 atttctgcat agaaaggtgt ttgatcaaag atcatttgag cttcaccttc tgcgacttca
     3301 ttcaaaagag actcaccttt aacgatgacc aataattgac tatctgattc ttgttctgtg
     3361 tatccaatga atttactttc tactttgata tctgtcaata aagcagattg tacccccatt
     3421 gatttttcag tactgcgggc agagcgagca cgttcgcgtt gtgcatccat ttctttttca
     3481 aaaccagcat gatcaacttt cagtccttca tcttctgcca cctcttccgt taattctacc
     3541 gggaagccat atgtgtcgta tagtttgaag atgtcttttc ctgctagagt cgtcccgttt
     3601 tcgtttttta cttcttggat cacttgattt aagatttcca aaccttcatt gattgtttca
     3661 ttgaaacgtt cttcttctgt acggataact ttttcaataa attctttttg ttgaagtact
     3721 tcaggataat aacttaccat gatttcccca acaacaggga ctaaacgata taagaatgct
     3781 tgttcaatac ctaatttttt cccatgcatg acagcacgac gaagcaaacg gcgtaagaca
     3841 taccctctcc cttcattcga tggcaatgca ccgtcgccaa tagcaaatga aagggcgcga
     3901 atatgatctg cgattacttt gtaagaaata tctgttactg ggttttcacc atattttact
     3961 tgtttgctta gcttttctac ggcgtggata ataggcataa acaagtctgt ctcaaagttc
     4021 gttgacgcat cttgaacgat tgagaccatg cgttccaatc ccatccccgt atcaatgttt
     4081 ttatgaggaa gtggttcaaa tgtgttatca ggcttatggt tgaattctga aaatactaag
     4141 ttccagattt ctaaataacg ttcattttct cctcctggat agttttcagg atcgtcttct
     4201 gctaaatcgt tataagcttc tccacggtca tagaaaattt ccgtatccgg accacatggg
     4261 cctgcaccga tatcccagaa gttgtcttct acatcaataa tgtgatctag tggcaggcca
     4321 acttcttcat gccagatgcg ttttgcttct gtatcttttg gataaactgt tacataaagt
     4381 ttttctggat cgaatgccat ccattttgga tcagtcaaaa attcccatgc ccagtggatg
     4441 gcttcatttt tgaaatagtc accaatcgaa aaattaccca gcatctcaaa catcgtatgg
     4501 tgccgtgctg ttttaccgac attttcgata tcatttgttc gaatagattt ttgggcattt
     4561 gtgattcttg gattttctgg cacaactgaa ccatcaaaat attttttcaa tgttgcaaca
     4621 ccagagttga tccacaataa ggttggatcg ttcacgggga tcaaagacgc actaggttca
     4681 atcgtatgtc ctttagattg gaagaaatca agaaacattt gtcgaacttg tgcactactt
     4741 aattgtttca tgtcattacc tgctttctat aaattaaata caaaaaaaca ccatcacagc
     4801 caaggacgaa aactcgcggt accaccttga ttgcaatgat gtgtaatcat tacctcttaa
     4861 actcgataac gctgagctgc gtcggtattt ctaccaaaat agaaaaaggg agcaatttga
     4921 ttagtgacta ttcttttttc agccaagaaa gaattttctg tgagtcttaa aaaacaaatc
     4981 atatcctttt cgtacttaat tagtatacgc attctattga tcactgtcaa tatctcaatt
     5041 tttattttca tttccttttg gtcatactcc acgatttttg gtacgataag aataaaggaa
     5101 tcaatacatc tctgctattc atgacaagga ggaatatgat gaaaaaaata gttggagcgg
     5161 atgaatggat acgcgcagct agctgctttt tgagatatca agtttttgta ttagaacaag
     5221 ggattcctgc agaactagaa ttcgacggaa aagatatccc gcaacaaacc tattatctat
     5281 ttcttgatca aaaaagacca gtagctactt tgcgttatga aaagaaaaca cctacttgct
     5341 tcaagccgga acgattttgc gtagcaaaag aataccgagg aaaagggtta ggcggtcagt
     5401 tgcttgaagc tgctgaaaaa cgggcggttt cagaaggttg tcagactagt tatgtagtag
     5461 ctgaaatgac ggctgtagat ttttatgaaa aacaagggta tcatacttgc acagaacctt
     5521 ttattgaaga tgaagtactt tgtatcggaa tgaaaaaaag tctagcttaa aaacaagaga
     5581 ttgtgacacg ccttttgtca caacctcttg ttttttattt atgtgaattt ttcttatttt
     5641 tacgagcagt acggctttgc tgtctgcgtt cgattttgcg ttctttttta tttttttctg
     5701 caattgccca atcgattttt ttcttataac caggcttgat ctttttcttt ttcttcttca
     5761 caagaccaat cagcgtggga tctaatacat cttttgattt ttctcgtttg gcacgtctat
     5821 tacgatcgta tgttttgacg atttccccgt ttttgatttc ttttggttca aatgagacgc
     5881 ccaatttttc tatcgcatca atggcttgct catcatttgg cgaatataac gtaatggccg
     5941 ttccttttaa gccattacgt ccagtacgtc cgacacgatg gataaagaag tctagatctt
     6001 ctggaatttc tgcattaata acatgagaga caccttcgat atcgatccct ctagcagcta
     6061 aatcagtcgc tacaacatat tgatactcca gattctgtac ttgtcgcatc actcgttttc
     6121 tctctcttgg ggtgatgtct ccatgaatct ttgctacttt caagccttgg tttttcaagt
     6181 aatcggcaat ttcatccaca tgctgttttg tattggcaaa tacgattgct aaataaggat
     6241 gaccaattgt cagcagttga taaatcattt gattctcatc tttacctttt gttgaaatca
     6301 gccaattttc aatgtcttca gaaataactg cttttggttt gatttcttca atgattggat
     6361 tttccatgta ttttcgtaag aatggacgaa gtttttctgg aatagtggca gaaaaaacta
     6421 aaaactgcag cttttctggt aatcgacttg caatttggtc tacttcattt aaaaaaccca
     6481 tatccaatgt catatccgct tcatcgacaa caaatgcaaa tgcggtgtga atcttcagcg
     6541 cttgttcatt catcatgtct aatattctac caggcgtgcc tactacaacc tgtggctgtt
     6601 ggtgatgcag acgatttaat tgacgctgtt tgtctgtacc gccgacaaaa ttcgagacac
     6661 ggatctccgg tgtagaaaat ttagcgattt gtgtagctgc ttcatagatc tgtgcagcta
     6721 gttccctact tggagctgta ataacaactt gtacttcctc tttttctgca ttgatttttt
     6781 caaacaaagg caacaaaaac gtatgtgttt ttcctgtacc agtttgagat tgcccaatga
     6841 tattttttcc ttttgcaatt aaaggaatca aacgttcctg gacctctgtg ggctgtttga
     6901 agccctttgc ggttaacgct tcattgataa acggtttgaa gcggaatata tcaaaattat
     6961 tcattcgtgc acctcttctc tttaaattat cacgactttg gcattataac acagacaaag
     7021 cgtttttgct cagctaattt cgagaatcat catcctttgt ttggccaatc gtgctaacag
     7081 tatatgtgtg actagctttt tcataaaaca gaatccgttt ttttctcaaa aatgatagca
     7141 agatatttaa aataaaaata ccttgtagta acaagacacc gagagaaatg aatactgcac
     7201 tcatctgcaa caccgtattc gttgtcgaat tcatgatttg ctgaagcaac tgccccaacc
     7261 agcttaaacc ataaaaaaga aagtacagat acccgtagcg gatcatcaat gcttttaaag
     7321 aaattcgttc gcggtcttct tccaccaaac ggatacgcac aatttttttg ccaagtgtcc
     7381 atccattggt tataaacggt acaatcataa agtatacccc tacttctaat aaaagccacc
     7441 agatatgaga agtaactgcc cctgaatcga acgcaatttt aaagaaaatc gccgctacca
     7501 aattaataag agataaaaat gtccagtcga tcaaataagc gaccaagcgt cgagtcaatg
     7561 agacagtctt tcctttttca taagaaagtt catccattcg tcttcttgta ggaagcataa
     7621 atgtaaataa cggcgtaacg acaaaaccaa tgattccacc caatgtattt gtcactaaat
     7681 cattcacatc aaataagcgg tatggtcttg ggtagataaa ataaagtcca cttagctgcg
     7741 tcaattcaaa aaatagagaa aggcagaaac tagcaatcat cgttttcata aaagaaagcc
     7801 ggaaataata tcttaaataa atgccaaacg gtatgagtaa taaaacatta aaagctggtt
     7861 ccaaaaatac actctgcttc attgccggca ggtaggtact aggttcagac aagaccaata
     7921 ccgtgttgtc tctgaaatgt tgccatgaag caaataaatg cagctccatc tttggccctg
     7981 tcatttgggc aaccgtctcc tttgccggaa gtggtaagat caccaaaaaa tacgcacaca
     8041 ataaataaaa aacgaaagaa tacaaaataa ctgcccttgt tactagaaat gttccatact
     8101 ttcgatattc ataaataaaa aaaacagatg agatcgctaa ggctaaaaat ggaaaaacaa
     8161 tcagcgcaaa acgaatcggt tcgatataac ttgccatatg actcctcctt gattctttgt
     8221 atctctctta tcttagcagt cctttgctgt ttcaagcaag taacgaggct attatttctc
     8281 atttctttca aagtaaagta agataaacac aaagggggtg tcgagatgct gaatatgtac
     8341 tttgtttttg gcgtacctat ttttttactg ttcctttatg ctacgatcgc ttatgtaaga
     8401 aaaagaacta caattcatta tttaggattt attctcctga ttatttcggg tttcatgcta
     8461 gtcttcaatc ttcagacttg gcagcaagca cttcttgaaa tggataaaat gactccacat
     8521 gctttgtcta aagtgcttgg ctaccctgtc tatctgattt ggcttcccat tttcatttct
     8581 ggttgcttgg ttttattaaa tatttatcgc ggcgtacgcc gaatagttca gttacgcaaa
     8641 tcaaagtaaa agacagaaat cggttgagcc cgatttctgt cttttacttt gctatttcat
     8701 cgtagacaaa acgacattta cgtgatcaaa attaacgagt acttttgaca tttcttccac
     8761 gtactcctca tttaaataaa atattttgtt tggcagagaa gtgcagttct aataaaattc
     8821 gaatgtttta gctatctacg ttttaaatag agataaatcg ggataccagc aagtgtcaat
     8881 actatcccca tcgaagctaa aaaggtctga gtgataagcg tgcttgatac gataaaagtc
     8941 cctccaatga ttgctactag tggaataaac ggatacattg gaactttgta tggtcgaaac
     9001 aaatcaggtt cttttttacg aagaagaatg acaccaacaa agaccatcgt ataaaaaatc
     9061 caaatgacaa agatcagcat atccgttagt gtatcaaaac caccgatcat catcatgcca
     9121 atagcgatga ctagctctaa gatacctgcg gctacaggaa ctttagtttt atcatgaagt
     9181 ttagcaaaga gcttgctaaa aggcaggttg ttttcttttg ccataacata agggagacgc
     9241 attccagtaa gggtatagcc attgatggtt ccataaacag aaatcaagat accaactgtg
     9301 accagtcttc caccgaagcc gccaaaaatc atttttgcaa cgtcacttgc cgcattgctg
     9361 tttcctgcga ctccgtcgat agatgcggtt cttaagaaca ccgcatttac taataaataa
     9421 acgatcatga ttccgatgat tcctaaagaa attgcttttg gcaaatcttt cgccggcttt
     9481 ttcaattctc ccgaaatatt tcctacgtgt atccaaccgt cgtatgcgaa catcgttgct
     9541 agcaagcccg ctcccaaggc tgagaaaaat gaaagatttt ctcctgcttg gatcggaaac
     9601 agttggaagt ctactccttc ttgtcggaac aaaccgaaaa tgacgatcac aaacaaagga
     9661 ataagcttgc agacaagcgt aatcgactga aaagcccctc cagctttaga acctaaaaaa
     9721 ttgatcaaca ggatcgatac agcagctgta acagctacgg gaacaatcat tgactgggaa
     9781 agaccaaata gattgacgaa ctgtgtaccg aaaatgatcg acaaggctgc tacatttgct
     9841 ggaaaataaa tcacgacttg cgcccagccc aataaaaaag ctgctgtatt tccgtaaatc
     9901 cgttcgatat atttaatcat ccctcctgtt tctggaatag cagctgccag ttcagctcct
     9961 gttagtcctg cacagacaga aatcatacca ccgagaaacc aagaaaacat atgcaaactg
    10021 gcggatcctg taacttctgc tacgcttgcg gctttaaaaa atacccctgc accgatcact
    10081 gtgcccataa cagtcgataa agcagtaaaa aatcccatcg tacgttttaa ttcattttct
    10141 atttttactt cttttactgg cgtggcttga tattcttcca tcccttgtcc cctttcatac
    10201 catagccaat cccaaaagta taaaaaactt tttggatgtg gttcttgttt tataccgtct
    10261 ctaccaattt ttctttcagt ttttccaaca tatcctttgt catcacatct aagtcgtatt
    10321 tcggcgaaaa gccccattcc tcttgcgcac aagaaacatc tagtgaattt ggccaagatt
    10381 ctgctattga ctgacgtaca ggatccacat cataatccat ttcaaaatct ggtatgattt
    10441 tttgaataga tgctttgatc tcttccgggt caaaagacat cgccgatata ttgaaggcat
    10501 ttcgatgaac caatcgatca ggatctgcat tcatgagttt tataatggca tcgatcgcat
    10561 ctggcatata catcatatcc attgatgtac cttgatcaat gaaactagtg tactttcctt
    10621 ctttgattgc tgaataataa atatcgactg cgtagtctgt cgtaccgcct cctggcaagg
    10681 ttgcataaga aatcaatcca gggaaacgga caccacgagt atctacgcca tatttcacat
    10741 aataataatc acatagtagt tctccagcta ccttcgttac cccatacatg gttgtaggac
    10801 gctgaagagt atcttgtggg gtatgatctt ttggagtatt tggaccaaac gcgccgattg
    10861 aacttggcgt aaaaaatttc agctggaatt ctctagcgac ttccaatgca ttgaccagcc
    10921 cagtcacgtt caaattccag gctaattgtg gttttgcttc tgcaacagct gacaaaagag
    10981 cagccaaatg aatcaatgtg tccacttgat attgttctgc tatgctgcgc attttttcgt
    11041 aatccattac atctaggatc tcaaagatat cgtttttctc aatgacttca ggcatgcgta
    11101 tatctgtcgc aatgacattt tcactgccta gttcttgacg tagccgtgtg atcaactctg
    11161 aaccaatttg tcctaaacat cctgtcacca atactttttt catgatgatc ctccctgatc
    11221 ttttttacta ctatcttcag atgattaaca atatatgcgt gaacatgttc tctaatttta
    11281 aatagacgaa aaaactaaat ttagttaaat aatttccatt tcttttccta ttttttgata
    11341 aactgctact gcttcatcaa gcatttccct cgtatgttcc gcagtcggca tatttctgat
    11401 cctgcctgtc ccaagaggca cagttggata aacgattggt ttagcataaa tcccatgttc
    11461 gatcagcttc ttagagaaag tctgagtcac tttttcgtct cccaaaatga ctggagtaat
    11521 aggtgtttca gacataccga tgttatagcc tacttttttc aattcttctt taaaataatt
    11581 agcattttcc caaacttttt cgacaagttc cggatgttct tgcatcaaag taatcgaagc
    11641 caatgcagct gcagctgcac ctggagttag cgaagtagaa aataggaatg gccgacttct
    11701 agctttcaac cagtcgatta gtgttttcga tccagctaca taacccccaa caacacctat
    11761 agctttcgat aacgtgccca tttgcatatc gattttgtcg cttaatccaa aatgcttgac
    11821 ggttcctgca ccttttccag ttacgcctga gccgtgcgca tcatcgacgt aggtgatcag
    11881 tccatatttt tcaacaatcg gaataatttc cggcaatcgt gcaatgtctc catccatgga
    11941 gaaaacacca tctgtgatat acatgatttt tttatatttc ttactttcta tcgcttcctt
    12001 tgcttttttc tcaaggtctt tcatatcttg atgtttaatt cggatgattt ttgctccgga
    12061 taagcggcaa ccgtcaataa tagatgcatg gtttaattca tccgataaga tagcatcttc
    12121 ttttgtcatt actgcagaga tagcgcccat attgcagttg aatccagatt gaaaggcgat
    12181 tgctgcttct gtccctttga attctgcgat tttttcttct aattgctgat ggattttcag
    12241 cgaaccattg atcgttcgga ctgctcctgc tcctacacca taagtttcgg ttgcttcaat
    12301 acatgctttt ttcaattctt cgcgattggc aaatccaaga taattgttgg atgctagatt
    12361 gatcatcttt tttccgtcta cgatgataca agcaccattt tcactttcta gtatgtcgat
    12421 cgtattgtat aacccctttt ctttcagttg atccaattct ttctctaaat acattgttag
    12481 ttctttggaa gacattgtct ccacctccat attcatctgt cttgcagaac ataccaaaca
    12541 tatatcgtgt cattcatcta caaagcaagt atgctatatt tttgataacg cttacactga
    12601 ttatgagaat tatttaacca cattttttat ttacagtatc tttttacttt caaatgactt
    12661 atatagtatg aacaattacc ttttagataa tgatctattt tactctacct gggcagataa
    12721 atataacaca accattgata gaagcccttt cgttaaaatc aattgtattc tgaatattta
    12781 atttatttat tgcatagacc aataaaaata cataaaattt ccatcttcgt ttatcagcaa
    12841 tttttaattt tactctctga atatttctta aaagttttac ttgttgaatt cgaaaaaagg
    12901 actgtgacaa aagtcttgtc acagtcctat tcttccgaat a
//
LOCUS       DAZAID010000079        12898 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000079 DAZAID010000000
VERSION     DAZAID010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.080"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..3654
                     /locus_tag="ACG9BE_002149"
     CDS             127..3654
                     /locus_tag="ACG9BE_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFN3032882.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="ACG9BE_002150"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="ACG9BE_002150"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFN3032883.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="ACG9BE_002151"
     CDS             7442..7783
                     /locus_tag="ACG9BE_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFN3032884.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="ACG9BE_002152"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="ACG9BE_002152"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFN3032885.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /gene="pheT"
                     /locus_tag="ACG9BE_002153"
     CDS             9140..11560
                     /gene="pheT"
                     /locus_tag="ACG9BE_002153"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFN3032886.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACG9BE_002154"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACG9BE_002154"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFN3032887.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4381 a         2242 c         2737 g         3538 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttcta
//
LOCUS       DAZAID010000080        12733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000080 DAZAID010000000
VERSION     DAZAID010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.081"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG9BE_002155"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG9BE_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFN3032888.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG9BE_002156"
     CDS             complement(323..892)
                     /locus_tag="ACG9BE_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFN3032889.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG9BE_002157"
     CDS             1078..1980
                     /locus_tag="ACG9BE_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFN3032890.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG9BE_002158"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG9BE_002158"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFN3032891.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG9BE_002159"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG9BE_002159"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFN3032892.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACG9BE_002160"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACG9BE_002160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFN3032893.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="ACG9BE_002161"
     CDS             8548..8733
                     /locus_tag="ACG9BE_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFN3032894.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="ACG9BE_002162"
     CDS             complement(8795..9991)
                     /locus_tag="ACG9BE_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFN3032895.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="ACG9BE_002163"
     CDS             complement(10161..11597)
                     /locus_tag="ACG9BE_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFN3032896.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="ACG9BE_002164"
     CDS             11890..12699
                     /locus_tag="ACG9BE_002164"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3032897.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3801 a         2672 c         2250 g         4010 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tga
//
LOCUS       DAZAID010000081        12186 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000081 DAZAID010000000
VERSION     DAZAID010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.083"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..312)
                     /locus_tag="ACG9BE_002165"
     CDS             complement(148..312)
                     /locus_tag="ACG9BE_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032898.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            607..1935
                     /locus_tag="ACG9BE_002166"
     CDS             607..1935
                     /locus_tag="ACG9BE_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFN3032899.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2137..2970
                     /locus_tag="ACG9BE_002167"
     CDS             2137..2970
                     /locus_tag="ACG9BE_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFN3032900.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2986..3723
                     /locus_tag="ACG9BE_002168"
     CDS             2986..3723
                     /locus_tag="ACG9BE_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFN3032901.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3878..4846)
                     /locus_tag="ACG9BE_002169"
     CDS             complement(3878..4846)
                     /locus_tag="ACG9BE_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFN3032902.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5078..5911
                     /locus_tag="ACG9BE_002170"
     CDS             5078..5911
                     /locus_tag="ACG9BE_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFN3032903.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5945..6784
                     /locus_tag="ACG9BE_002171"
     CDS             5945..6784
                     /locus_tag="ACG9BE_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3032904.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6810..7370
                     /locus_tag="ACG9BE_002172"
     CDS             6810..7370
                     /locus_tag="ACG9BE_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3032905.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7569..9290
                     /locus_tag="ACG9BE_002173"
     CDS             7569..9290
                     /locus_tag="ACG9BE_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFN3032906.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9455..10597
                     /locus_tag="ACG9BE_002174"
     CDS             9455..10597
                     /locus_tag="ACG9BE_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFN3032907.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10613..11824
                     /gene="thiI"
                     /locus_tag="ACG9BE_002175"
     CDS             10613..11824
                     /gene="thiI"
                     /locus_tag="ACG9BE_002175"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFN3032908.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4153 a         2045 c         2531 g         3457 t
ORIGIN      
        1 actgccggag taaataaagc ttatgcaaag aaaaagaaat ccaatcactt gggtttcttt
       61 ttcttttatt attatgatga atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta
      121 cgaaagagta gagttggcta ctcataatca accatacttc ccatcatcgc taaatacgaa
      181 ataaatcagc attcctacac caatcaaagc acaagctgca ccaagaaaag ggatgacatt
      241 cccaaagtta tctgtattca taagtatgcc accacccaca aacaaaaagc cgccagcaat
      301 caggtttttc attttattca tctcctttct tacgttatag tatttccttc tttcaatgag
      361 tattttgaaa gctatcttat tgtatgatgt tcttctaggc ctcttcttta ttatacaggc
      421 cattttcgtt atctcataga aatattcctt tttatctgaa aactattgta taccgtatga
      481 catttttctt catgaagttg taagtatatt ctcattcaaa tgaaaatttc atcatagaag
      541 agattgtatt tctaaattct taagagtaaa atacgaagta taacttggat ttggaaggat
      601 gattggatga aagctattac gcctgatatg acaacaaaat ttaacaaaca gtttcaagca
      661 aatatcaaac agcaggcatt gcagcgggca gttgtgaaga acggaattac tgcttctgct
      721 gaaaatgttt cagcacatgt ggagaataca ccggtatttt ctatagattt ggctacgggg
      781 aaagtggcta atcaaatgca atcggggcgt tgctggatgt ttgctgcact gaacacattc
      841 cgtcataaga tgctgaatca attccattta aaagagtttg aattatcaca aaattacact
      901 tttttctggg ataaatatga aaaggcaaat tatttctatg aaaacatcat tgcaacagga
      961 aaagaggcaa tcgatagccg gaaagtagct ttcttactcg ctactcctca acaagatggc
     1021 ggccaatggg atatgatcgt ttcattattc caaaaatatg gcgttgtgcc taaatcggtg
     1081 atgccggaaa gtgcgaatag ttcaaattct cgagatttga ataactactt gaacaaatta
     1141 ctgcgtaaag atgcagtagt gttacggaaa atggttgctc aaggagaaac attagacgct
     1201 attgaagaaa agaaagaaga aatgctagaa agcatttata acttcttagc tatctcatta
     1261 ggaacacctc caaaagaatt cgattttgag taccgcgatg aagaaaagaa ctatcatctt
     1321 gaccgaggac tgacaccaca aattttttat gataaataca tcggtgtaaa attagatgat
     1381 tatgttagcg tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc ttatacagta
     1441 gagatgctag gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa
     1501 actttcaaaa aattagcgat tgctcagctt gaagaaggag aatctgtttg gtttggttgt
     1561 gatgtaggtc aatcttctac tagaactagt ggcatcatgg ccttagatgc ttattcaatg
     1621 gatgatctat ttgatacgga ttttacaatg actaaagctg aacgtttaga ttttggagaa
     1681 agtctgatga cccatgcgat ggtattgacc ggtgttgact tagtagacgg gcaatcaaca
     1741 aaatggaaag tcgaaaatag ctggggcgaa aaagttggaa agaatggctt ctttgttatg
     1801 agtgatgcct ggatggacga atatacgtac caaatcgttg taagaaaaga tttattgacg
     1861 aaagagcaat taaatgcgtt caatgaagaa ccaattgttc tatctccgtg ggatccaatg
     1921 ggtgctttag catagacgag tatttcaatg aagagtatgt agtaaagata aaaagagctg
     1981 gcttaagcca gctcttttta tctttaaagt ccattccctg ttttttatgg tccctgtgct
     2041 ataattctaa ataaaggata aggattggaa ataatccttt tatcattaaa aatatgatga
     2101 cgaagtagaa taaaattggg agtagggggg aaagtcatgg atatccaaac tgttttatct
     2161 gatctgaaat taaatggtaa agtgattcca gttgttggtg gcgatgttaa tcagacctat
     2221 cgtataaaaa ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga
     2281 ttttttgaag cagaagtaga cggattaaaa gaactgtcag cattcgtacg tgtaccggat
     2341 acgtatatgc taggtgaaac aagtgaaggc gcttatttgt taatggaatg gatcgagccg
     2401 ggaaaagggg atcaacgaga tcttgctgca gctttagcta atctccatca gcaaacagcg
     2461 ccacaatttg gttttcgtaa agataactat ttgggtacac ttgttcagaa aaattctttt
     2521 gaagaagatt ggtggacttt tttctttaaa gatcggcttg aatcccagat ttctttggca
     2581 gaagaaacga atcgatggaa tgtacagcgt caagaaaagt atctgcgatt taaagaacgt
     2641 gtattgaaat cggttgaacc aaaaaagatc actccacgcc tcttgcacgg agacctgtgg
     2701 agtggtaatg tgttctttga ccagcaaggg catccagttt ttgtcgaccc agctgtttct
     2761 tatgggaatc gggaacaaga tattgcaatg agccagttat tcggtggttt ccgaccggaa
     2821 tttttagatg cttatcaaac tatatttcca ttagaaaagg gatggaaaga ccgactgccg
     2881 atttaccagt tatattatct tttggcacat ttgaatatgt ttggagaaag ttacggttcc
     2941 caagtagatc aattactaga aaatttttaa gaggagggcg tgtttttggc aaaaaaatat
     3001 accaaaaagc atgaagtttc atattatgag tgtgacatca accaaacgat gacatttcct
     3061 tcgatgttag gagtagttat caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc
     3121 agtgaattcg tcaatagctt tggacttact tgggtcatca cgaattattc catggagatt
     3181 aatcgtctcc ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat ggagtacaat
     3241 aagtttttct gttatcggaa cttctggatc catgatgaag atgggaaaga attagtcaaa
     3301 atagaatctg ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa
     3361 gagatcattg ctccttttga gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt
     3421 gaaaagattg attcgggctt catgttgcca tatcgtgttc gtttttatga tatcgatagt
     3481 aatcagcatg ttaataatgc gatgtatttt aattggatca ttgacgtatt aggatatgac
     3541 tttttgacta cgcatgttcc agaaaaagta attattcgat ttgataaaga agtagagtac
     3601 gggaatgaga tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa
     3661 catgaaatcc gaatgcaaga acagctgtat tgtgaagcaa atattatatg gaaaaataaa
     3721 taaaaaagag gttgtgacag aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa
     3781 ttgtttctca aatttttgta aattttcgac ttagttttcg aagaagctat ttttgtcaca
     3841 gcctctcttt ttaagtttag ctattagata atgatgttta gttttggata aacgaaatca
     3901 attcttcacc cgacaaacca tagtttacag caatcaatag ttttaaacgt gctttttgac
     3961 tattaatact gttacagaaa ataatcccca ttttttctag ttcttttccc ccgccttcat
     4021 aatcataaac aggctctgcg attccattga aacaacgaga aacaagtact acagggattt
     4081 tttggacaag aagcttttcc aaagcagcta aggcttgtgg agggagattc cccgcaccta
     4141 aagcttcgat taccaaaccg tctactttcg tatgggcgat ggcttccaat aagtcccctt
     4201 gcatcccagc atatgcttta acgatcggga tagtcccatc tacagcggaa atgtccagtc
     4261 gctttgtttc taaaagttcc tgcaagaata aaattcgatt cttagttact aagccgatgg
     4321 gacccaatgt aggtgttcgg aaagtagcaa catttgtggt atgggtcttt gtcacgtagc
     4381 gtgcagaatg gatctcatcg ttcatgacca ccaaaacccc cttatctagc gcttcttcac
     4441 aacttgcgac acgaatcgca ctttcaaaat tataaaggcc gtcacttcca agttcattac
     4501 ttgaacgcat tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa
     4561 aaaaagcagt ttcttctaat gtatctgtac cgtgtgtgat caccactccg gatatacctg
     4621 atgcataagc ggacaagata cgttctttta ataccaacat gtgagaaggc atgatgtgtg
     4681 gtgagggaag attgaagata tcttcaacta ataattctac atgcttgggg attgtgattt
     4741 ctgcatctaa taatgggttt gccacatcag gactaacacc gcccgtgagc atgttttctt
     4801 tcatggcgat cgttccgccg gtgtgtagta caagtatttt tttcatttta cagacttcct
     4861 tacgaataat tttgcatagc cgttaaatct tattgtatac tatataaaag agttttgcta
     4921 tcagtgaaga ataacgaata gcctaactta tcataaaaaa gctgacctta ttaaaatgga
     4981 aaagactcaa ccttgacctc ctaatacaat ggagtatctg gttgagtttc ttttacaaga
     5041 aagcagccaa ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg gattaacatc
     5101 gtttaaagaa catgacaaat taactggtaa aaagaataac actctttatg aatatgcagg
     5161 gtttctaccc ttagtggaat tagatactgc ctattatgga attccccgaa agtcctcagt
     5221 tgagaattgg ataagtgaag tacctgaatc atttcgcttt gtagtcaaag tctatcaagg
     5281 aatcagcggt caaggagact ggaagcctta ttatcagacc gaagaagaga tgattgcgca
     5341 ttttttagat gttatgtcgc caatggtaga tgctggaaag atgttttgct ttttagtcca
     5401 attcccctca tcattcaaat gtacgaaaga aaatgtccag tacttgcgca aacttagaaa
     5461 gtggtttgag ggttatcctg tttctataga attgcgggat tattcttggt acagcgatga
     5521 atttcttgaa cgtacaagaa actttatgtc tagagccgct ttttctttag ccatcatcga
     5581 tgagccacag ttgctgaata caacgattcc ttttgatcca tttgtcacca atccgaactt
     5641 tgctcttttt cgattccatg gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg
     5701 gcgtaaaaaa agaacgttat atcgttattc agaaaaagaa ctgcaagaac tagcacatga
     5761 agtcaaagta gttgattcta agacaaaaga ggtaggtgtg atttttaata ataattctgg
     5821 tggtgatgcg gctgaaaatg cattagcact gcaaaaaata ttaaatatca agccagatgg
     5881 actcaatcca acccaaatgg atctgtttta agggcttatt ttattcaaga aaggggcgaa
     5941 gtctatgatc aaagctattt tttttgatat tgacggaaca ttggtcacca atcgttcaaa
     6001 agcgttggaa tcaacaaaac aagcaattga atatgctcgg aaaaacggca ttttatgcgg
     6061 tgtagcaacg gggcgcagcc cagtaaagat caaagaaatc attgatgaat tggaattaga
     6121 tatgtatgtc gtgtataatg gtcaactggt ttttactgca gatcgcacga ttatcgacca
     6181 cccttttgaa caaaaagtat tggaacatat tgttgaattt gcagatgaga accatcgaca
     6241 aatcgttttt ggtgcgagaa atcggttaga tggatcaacg acgatgctat taggacaatc
     6301 gatttttatc aagcgactag tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa
     6361 aaaaatcctc caggttttta gccctcaccg acaaaaggat cgttatgaaa cgttagagat
     6421 attaaaagaa ccaatttatc aatgtatatt gctcagtccg gagtcagaac agaaaaagtt
     6481 agaacatcgc tttcccgagt gtacgtttca gcgttcaaat acgtatactg tagatatcat
     6541 cccaaaaggt ggttcaaaat tactaggcat ccaagctttc gcaaatgctg tcgaaattga
     6601 aatggaagaa attatggcat tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt
     6661 aggaattgga gtggccatgg ggaatgctca gatcgaagta aaacatgctg cagactttgt
     6721 gactcaatca aatgaagaag atgggatata cgaaggactg aaacacttcg gtctgattta
     6781 ttagtcaatg aaagaaggaa aaaatcagta tggaagcaga gacacctttt caaaaagtag
     6841 aaacattcca caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa
     6901 atgaagctca tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta
     6961 cttcacctaa taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata
     7021 aagcagaaga aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag
     7081 tagatgcact gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag
     7141 tgcctgatcg aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg
     7201 gaaaacctca ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg
     7261 aatatgctcc agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg
     7321 ctaagaagaa atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc
     7381 actatttttg ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat
     7441 gcatgaaaat agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt
     7501 tggatttatg ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg
     7561 aaccataaat gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca
     7621 ttgtcgtctt tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct
     7681 tagaaaaacg caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta
     7741 aaaaaatgca tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga
     7801 atgacatatc tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc
     7861 tcaatgaaag ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa
     7921 tggaccaaat ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa
     7981 cggaagaacg caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag
     8041 aaaaagtcag agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga
     8101 tcaaagtgat cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg
     8161 ttgaagcccg cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga
     8221 tgaaaaaaat tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag
     8281 aaatcagtga tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc
     8341 ttgctgaaga tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa
     8401 aaacagaagt tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt
     8461 tatatgatat cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa
     8521 cgattgcaga gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg
     8581 atcacacatc tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc
     8641 aaaccgaagt ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac
     8701 acgaaattcc ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag
     8761 atatcgaaaa gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa
     8821 aagaagcgca agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg
     8881 tagaaaaaca acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta
     8941 ctgaccgtat tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg
     9001 tcaatcgttt agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg
     9061 atttagtaga tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc
     9121 atacacacga agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat
     9181 atcactatca agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg
     9241 ctttcaaacg catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag
     9301 ttagtaaacc agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt
     9361 tgaaaattga aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat
     9421 aatagaaaag aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac
     9481 aaccccaatc tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg
     9541 gaatccatca agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag
     9601 aaagcagatc gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg
     9661 aacagaagga gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa
     9721 tcatatgatc atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa
     9781 agaattagga atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga
     9841 cgaattgaaa gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa
     9901 tgaagtagga tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa
     9961 aattcatttc catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt
    10021 gacagaaaga gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac
    10081 tggtttcatt tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca
    10141 agaaagaaat cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc
    10201 tttacgttta tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt
    10261 agctctaatc aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga
    10321 ctatgcaccg catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca
    10381 tgccttagaa gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca
    10441 tatggcaagc agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt
    10501 tcgaatcagc ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt
    10561 caagcagctt tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata
    10621 cagtgaaatt atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt
    10681 tatcatgcaa ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat
    10741 ccatgctgat cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat
    10801 tccaaaacta gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa
    10861 agatgtcgaa gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa
    10921 agaaacattt aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga
    10981 attgaatcaa acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat
    11041 gcgtcaacca gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta
    11101 tgaaacgatc aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat
    11161 gctttctggg gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga
    11221 aattgaagct gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc
    11281 aaaagatcta acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc
    11341 atttaccgag attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat
    11401 tactcgtcga atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt
    11461 cattttgaat ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc
    11521 tatcaatgaa gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga
    11581 aatcattgaa gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga
    11641 ttgctgtacg atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca
    11701 tcaatatgaa gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa
    11761 gatggaagag atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct
    11821 ataagaggtt atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata
    11881 gctccatgta ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt
    11941 atcaagaagt tatggaaaag ccggttcttt tttactaact gaaaaataaa attttattta
    12001 gagatcaagc tacagttgat aaatgatata aaaaagagaa tttcccatga aatagcgagg
    12061 ttgtgacaga agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa
    12121 atttttgtga aatttcggct tatttttcga agaagttact tctggaacac cgtgtatcag
    12181 aggttg
//
LOCUS       DAZAID010000082        12175 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000082 DAZAID010000000
VERSION     DAZAID010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.084"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(131..688)
                     /gene="frr"
                     /locus_tag="ACG9BE_002176"
     CDS             complement(131..688)
                     /gene="frr"
                     /locus_tag="ACG9BE_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFN3032909.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(691..1413)
                     /gene="pyrH"
                     /locus_tag="ACG9BE_002177"
     CDS             complement(691..1413)
                     /gene="pyrH"
                     /locus_tag="ACG9BE_002177"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFN3032910.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1549..2430)
                     /gene="tsf"
                     /locus_tag="ACG9BE_002178"
     CDS             complement(1549..2430)
                     /gene="tsf"
                     /locus_tag="ACG9BE_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFN3032911.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2529..3311)
                     /gene="rpsB"
                     /locus_tag="ACG9BE_002179"
     CDS             complement(2529..3311)
                     /gene="rpsB"
                     /locus_tag="ACG9BE_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFN3032912.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3670..4149
                     /locus_tag="ACG9BE_002180"
     CDS             3670..4149
                     /locus_tag="ACG9BE_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN3032913.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4366..5457
                     /gene="phnW"
                     /locus_tag="ACG9BE_002181"
                     /pseudo
     CDS             4366..5457
                     /gene="phnW"
                     /locus_tag="ACG9BE_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            5450..6235
                     /gene="phnX"
                     /locus_tag="ACG9BE_002182"
     CDS             5450..6235
                     /gene="phnX"
                     /locus_tag="ACG9BE_002182"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFN3032914.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6581..8272)
                     /gene="argS"
                     /locus_tag="ACG9BE_002183"
     CDS             complement(6581..8272)
                     /gene="argS"
                     /locus_tag="ACG9BE_002183"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFN3032915.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8694..9641)
                     /gene="arcC"
                     /locus_tag="ACG9BE_002184"
     CDS             complement(8694..9641)
                     /gene="arcC"
                     /locus_tag="ACG9BE_002184"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFN3032916.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9756..10775)
                     /gene="argF"
                     /locus_tag="ACG9BE_002185"
     CDS             complement(9756..10775)
                     /gene="argF"
                     /locus_tag="ACG9BE_002185"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFN3032917.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10866..12095)
                     /gene="arcA"
                     /locus_tag="ACG9BE_002186"
     CDS             complement(10866..12095)
                     /gene="arcA"
                     /locus_tag="ACG9BE_002186"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFN3032918.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3336 a         2531 c         2187 g         4121 t
ORIGIN      
        1 tttcctatga aatcattgtt tttttattta aaaactgaat aattttttct ttttttaatt
       61 tttaaataaa agaaatataa ataaaaacag aggaagcgga atgatccgcc cctctgtttt
      121 tatgatttta ttaaacttcc aataattctt tttctttatc agcagcgatc ttatctactt
      181 ctttaatgct gttatctgtc aatgtttgta cgtctttttc taatccgcgc aaatcatctt
      241 cggtaatatc accatttttt tgttgttttt tatattcatc gatcgcatca cgacggatgt
      301 tacgaatcgc gatttttgca ttttctgctt cttttttcac atctttagct aattcttttc
      361 tgcgttcttc tgtcagttgc gggataacta agcgaatgac atttccatca ttggttggac
      421 tgattccgat atcacttgcc ataatagctt tttcaacatc ttgaagaatg ctcttgtcaa
      481 aaggtgtgat cattaaaaca cgtgcttctg gaatttggat tgatgccatt tggttcaatg
      541 gtgttggcgc accgtagtag ttcacagtaa tacggtccaa aaggctagcg ttcgcacggc
      601 ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc tgcttttttc attttttctt
      661 ttgcttctgt cataattgca tctgccattt ttatttcccc cttacagttg ttccgatatt
      721 ttcgcctaag attgcacggc gaatatttcc gtgttcattt aaattgaaga caagcagtgg
      781 aatatcatta tccatgctta gtgaacttgc agttgaatcc atgacttgta atcctttggc
      841 aataacttcc agatgagtca gttcttcaaa ttttactgca tttgcatcta ctttcggatc
      901 agcagaataa acaccgtcta cattgttttt agccatcagg ataacatctg cgccgatttc
      961 tgccgctcgt aatgctgctg ttgtgtctgt cgagaagtac gggttgccgg taccgcctgc
     1021 aaagataacg atccgtcctt tttctaaatg acgttctgct ttacggcgaa tatatggctc
     1081 tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc ggtacaccca cgttttccaa
     1141 cgtatcctgt aaagctaagg cattcataac tgtagctaac atacccatgt aatcagcttg
     1201 agcacgttcc atgcccattt gtgctccgat ttgtccgcgc cagatatttc cgccacccac
     1261 aacgattgcc atttcaacgc ccagctcgtg aacttctttg atttcttgga tgatttcttt
     1321 gattactggt ggtttgattc caaatcctgc atccccagct aaagcttctc cactcaattt
     1381 taagactaca cgctgatatt tcggtgtgac catgttcatt cctccaatat tttctattgc
     1441 cattttacca tacttagatg atttcgacac tctaagactg gtttttcttg ttttgcttaa
     1501 aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt caacagaatt attttttcac
     1561 ttggctcata acttcttcta caaagttatc ttcgcgtttt tcgatacctt cgccaacttc
     1621 gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca acgtattttt caacagtcat
     1681 atcaggatct ttaacgaatg gttggtccac caaagcgatt tctgctttga atttcttcaa
     1741 gcgtccttct accatttttt caacgatatt cgctggtttg ccttcgttca atgcttgttc
     1801 tgtaagaaca gttttttcat gttctaattc agcttctgga atttgtgatt cgtttacata
     1861 acgaggattg atagctgcta cgtgcatagc tacgtctctt gcaactgttt cgtcagttgt
     1921 tccatctaga acagtcaata cggcaatacg tccgcccata tgcaagtatc caccaaatgc
     1981 agcattgtcg tctttttcta caacttcaaa gcgacggaag ctgatttttt ctccgataac
     2041 ttgagttgct tcgatcaagt catattcaat tgtaccttta tctgttttga ttttcattgc
     2101 agcttccatg tctgctggtt tgttttcagc aacaagttct gcaatttctt ttactaaatc
     2161 ttggaacatt tcgtttttag aaacaaagtc tgtttctgag tttacttcaa cgattgctgc
     2221 agtgttacct ttcacagcaa ctgaagccaa tccttcagct gcaatgcggt catttttctt
     2281 cgcagccttt gccattcctt tttcacgtaa aagatcaact gctttttcca tgtcgccttc
     2341 tacttcaact aatgcttttt tcgcatccat cattcctacg ccagtcattt cgcgtagttc
     2401 ttttaccatt ttagctgtaa cgtctgccat gtttaactcc tcctgtgtgt ttttcttttt
     2461 aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca gcctttgaaa cagctccatc
     2521 attcaaaatt attctgctga agaattgtcg ccttcaacaa catctacgat ttcttcgatt
     2581 gatgttgctg tgttttcttc tgtgaaatct tcttcaacta cttgatcttc accttggttt
     2641 ccttcgatga aagcatcagc catttttgaa gtaatcaatt taacggcacg aattgcatcg
     2701 tcgtttgaag ggattactac gtcgatttca tctggatcgc agtttgtatc aaccatagct
     2761 acgattggga tatttaattt gtgtgcttct tgaacagcaa tacgttcttt acgtgggtca
     2821 acgatgtaca ttacatctgg gattcttggc atatcagcga taccgcctaa gaatttttca
     2881 agacgttcac gttctttgtt caaaccagca acttcttttt taggtagaac ttcaaaagtt
     2941 ccatcttctt ccattgcatt gatttgtttc aaacggctga tacgtttttg gatagtatcc
     3001 cagtttgtca atgttccgcc taaccaacga tggttaacga agtattgtcc tgcacgagtt
     3061 gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac ctacgaataa tgctacgccg
     3121 ccttcttcag caacgctctt catgtaatca taagctgcat ctactaattt aactgttttt
     3181 tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga tatatttctt catttttgga
     3241 ttccagcgac gagtttggtg accaaagtgt acgccggctt ctagtaattg tttcatagaa
     3301 attactgcca tgtttgtttc ctccgatttg gttttatttt tcctcttctt atcttctacc
     3361 ttgccggcaa actttttcaa gcacctaccg acaatccaat aagaatgtgg atttgctgat
     3421 gcataaagca ccctcagcta ttatacagac aaaagatccg tatcgcaaga gcaaatatac
     3481 ttttctgctc aacattcaat agtttacctg aaagataaaa agaattcaag aaaaacagta
     3541 gatttctctt tgtttcttta ttatcctttt tccataaaat aattttaggt ataaaagacg
     3601 aatattattg ggcaatcctt cttttttttg ttatgataca tgtgaaatct ttcgtaaagg
     3661 agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta aacaagtcat tgaagaattc
     3721 ccgatccgta cgcaagaaga actattaaac aaacttgggc attatggcgt taccgccacg
     3781 caggcaacga tttctcgaga tatccgtgat ctaaaaatcg tgaaggcacc tgatgaaaat
     3841 ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc ctgagactag gactgaggaa
     3901 gaggatcggc tggttcaaat gattgaagac attgtattga aagtcgaacg agtccacttt
     3961 ttgacgatcg tcaatacctt gccagacaat gctcatttat ttgcatctgt tttagatgag
     4021 atcaaacctc cctctattgt cagtacaatt gctggattcg atacgatcat ggtcatctcg
     4081 gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc accatccaac tgaagtatca
     4141 cttttttaat cagagcacgt attcctaata tggaatgcgt gtttttttat tttcaatatt
     4201 ttccttatta aataaacctt tacactcttt acaaaaatac atcatttttt attcaatttt
     4261 ttcaacacct tgattgaatc acaataatca tcatttatat tggatgttaa caagagcagc
     4321 ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa atcatatgta taaattatta
     4381 actcctggac cattgacgac ttctgaaaca gtgaaaaatg ctatgctgat cgatcattgt
     4441 acttgggatc aagaatacaa agaaattaca caatggataa gaaaaaaatt attggatctt
     4501 gctcaagtat cggaaggaat atacacaacg gtcttaatgc aaggtagcgg ctcctttggg
     4561 gtagaatcgg tattaagttc tgtgatcagg ccagaagata ctgtactgat ttgttcaaac
     4621 ggcgcatatg gacaacgaat gatcaacatg tgcgaatgtc tgaaactgaa ctataccgtt
     4681 tatgaggtat cgtaacatca aatcctccaa gctcatgtga ttacacagct gattgaagag
     4741 aatccctcta tctccgcact agcaatggtc catagcgaga ctacttccgg tattttgaat
     4801 aatttggaag agattgctgc tgtcacacaa gaaaaagatc ttttgtttat agtggacgcg
     4861 atgtcaagct ttggtgggat accgattcgt gtagccgaac tagggattga ttttttgatt
     4921 tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt cttttgtact tgctaataaa
     4981 gcggaactag aaaagcgaaa aaatgtgagt cgaagtttgt cacttgatct atatgaccag
     5041 taccgcgtga tggaaaacga gaatggcaaa tggcgtttca cttcccctac ccatacggtt
     5101 ttggcttttc aacaagcgct gaaggaacta gaggcagaag gaggaatcga aactcgatat
     5161 tcacgttatc tcgccaataa tcatgtacta cgacaaaaaa tgacagagat ggggttccag
     5221 cctttcattc aagaatatca ggggccattc atcacctctt ttctttaccc taaggactgg
     5281 cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa acggatttgt gatctatccc
     5341 gggaaaatca gcgatcatga atgttttcgt atcggaaata ttggcgagat ctatccggaa
     5401 gatattttgg aactgacagc aattatcaaa gactataagg agaacgttaa tgattaaaac
     5461 tgtaattttt gattgggcag gaacaactgt ggactttggc tgtatggctc ctgtccacgc
     5521 ttttagaaat gcttttctag aaaaaggaat ccaactgact gacaaagaaa tccgcgaacc
     5581 gatgggaaaa ttaaaatggg atcatatcca acagcttctt tccctccctt ctgtcaaaaa
     5641 acaatggata gctatttatg gacaactacc tcaggaaaaa gaggtggagg aattgtatca
     5701 acaatttgaa cattatctgt ttaaagattt ggtcaagcat tctaccttga aacccgacac
     5761 attggaaacg atcgaacggt tgagaaacgc tgggatcaat attggttcca cgactggatt
     5821 tactgctcct atgatgacaa tcgtagcaga aaccgcaaaa aaagaagggt attcaccaga
     5881 cttcatggca acttctgaag acgtcgaagg gtatggtcga ccgtttcctt atatgatcca
     5941 taaaaacatg cagtacttca aaaacaagtc ggttgatgaa gtaatcaaag tcggagatac
     6001 ggtctcagat atccaggaag ggaaaaatgc cggagtgttg acagtaggtg taattgaagg
     6061 tagttcgcta atggggattt cttatgatga gtaccaaaaa ttagagatgg ggaaaaaaga
     6121 tcgtttaaaa cagtctgttg ctcttagatt ccaacaagct ggcgctgata tttgtgtaaa
     6181 caatttaaaa gagttaacag aatacatttt gtacgctgac aagaatccag aatgactttt
     6241 atacaaaatc agaggtcgtg aaaaagctgt actgcatcaa gtaataagaa cagccaaaca
     6301 aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc aagcttttga
     6361 acaccgttta ttcgaaaaaa aaagggagct gtgacacgac ttttgtcaca gtccccttat
     6421 ttacaaacaa acagtgttta agagacaacc tctccactaa acggtgttca ttcagctgac
     6481 tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct attttctctt ttgtttctct
     6541 aatactcgga tcagaacgct gatttcacat tctctttttc ttacatctta tctggtgcat
     6601 gaagtcctag caaacgaagg tcttctttca ataatacagt cactgcataa actagagcta
     6661 agcgtgcttc tttttgttca tcatcagcta aaatcttcgt atgagcatag tatttgttga
     6721 atgcttgtgc taatttgatt gcatgtttcg caatcactga cggttcgtat ttttccccag
     6781 cagacaagac cgtttcaggg tatttttgaa ctaatttaac cacttcccag ctggtatcgt
     6841 cattcaatgc atagtctgct gtttctgaag ggacaaatcc tgctttttcc aacaggctga
     6901 ctgcacgagc atgtgtatat tgcacataag gtccagtttc tccttcaaat cgaacaactt
     6961 cttccaggtt aaagtcaaat gtattcagtc gatcgttttt caaatcgtgg aaaatgactg
     7021 ccccaacccc aacttgttta gctacagcat ctttgttttc caaatcaggg tttttctcgc
     7081 tgatttgttc tttagcagat tggattgctt catttaatac ttcttctaat aagacgatct
     7141 ttcctttacg agttgataat tttttaccac cttgcgtgat caaaccaaat ggaatatggc
     7201 gcatatcgtc agaccagtca aagcccattt ccttcaagac agctttcaac tgtttaaaat
     7261 gatagctttg ttcattccct actacatata gagattgttt gaagtcgtaa gtacgtttac
     7321 ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt cgcaccgtct gacttcttaa
     7381 tcaacgctgg attcaggtca taagcagata ggtcaacgat ctctgcacct ttatcctcgt
     7441 tcagcaaatg tttttcttct aaaagtttga cgatttcatc catcttgtca ttgtagaaag
     7501 cttcaccgtt caacgaatcg aaacgtactt ccagcagatc atagatttta ttgaattctt
     7561 tcatcgattc atcacggaac cattgccata attgaatagc ctcttcatcg ccttcttcca
     7621 gacgtttgaa ccaagcacga gcttcttcat ttaattctgg ttctgtttca gctacttcat
     7681 ggaattggac atataaacga agcaattcat tgattggttc tgctttaaca gcttcttctg
     7741 taccccattt tttgtaagcg acgatcagtt ttccaaactg tgttccccag tcacctaaat
     7801 ggttgatgcg gattggctga taaccgattt tttccataat gaaaccgatc gaattaccga
     7861 taactgtcga acgcaaatgt cccattgaga ttggtttggc aatattagga gaagacatgt
     7921 cgattggtac tgttccttga ttcccgatat tgctatcccc ataatgttct ttttctttca
     7981 ctactgtttg caaaactttt ttgctgataa gttctttatt catgaaaaag ttcaagtaag
     8041 gtccgacaac ttcgattttt tcaaagttgg cagaatcgat tttttctgct aaatctgcag
     8101 cgatttgctg tggcgctttt cggtaaactt ttgctaatga aaaggctgga aatgcaacat
     8161 ctccgtgttc agctgatttt ggattttcta gtaattgttc gacttgttcc agtgttaaat
     8221 catctttgac agcgtcatag acagcttttg cgacaagatc tttattattc atctttattc
     8281 ctcctaaagt gctcccttga ttgttaaaca aaaaaaatct ctagcgacgg catacttagt
     8341 atgccatcac tagagacgag atttcccgcg gtaccactcc aattgtccta aaaaaggacc
     8401 agctccatct gatatcggta gacgaccgtc tgtttaaaac agaattctcc aagtgcgctt
     8461 cgttgtatct tcttcattcg gcttccacca cttccgaatt cgctagccaa gaatgataca
     8521 agtactctct tgttcaacaa atcttagtct tattaaactt tcttgaatta tatcagaaaa
     8581 aaaaacgaaa ggctagtcgt ttctgataaa aaaacaatcc agatggccta aaggagaaga
     8641 aataccacct ggatagctat aaatcatttc gtaaatacga aagacttaac aaattaatca
     8701 gctacgataa tcgttccttc ttcagaagct aaaagattct cgatattttc taaagaagtg
     8761 atgattgcct tagcgtttgg ttttgcttca acaaatgcaa tagctgcctc tactttcgga
     8821 agcatgcttc ctggagcaaa ttgcttttca tcaatgtatt gtttcatttc tgaaacagta
     8881 acagtttcta atttcttttg atctggtttt tggtaattga catacacatt gtctactcct
     8941 gtcaatacga tcagcaaatc cgcatcgatg atttcggcta atttttctga agcaaaatct
     9001 ttatcgatta cggcttctcg tccttctaat ccagtagctg tttctacaac aggaatccca
     9061 ccgccaccaa cagagactgt gataactcct tgatcgacca gcgtttcgat gacacgtgct
     9121 tctttaatcg agatgggttt aggggaagcg actacttttc tccatccacg tccagcatct
     9181 tctttgaatg tcacagtgga gtcagcattc atttgttctt tggcttcttc ttccgtgtag
     9241 aatggaccga ctggtttact tggatttttg aatgaaggat cattttcatc cacaataact
     9301 tgagtgacta aagaaaccac atctttatcg atcccttttt ccttcagtac ttcgcccatg
     9361 gcattttgta accagtaacc gatagatcct tctgtcattg caacacatgt atctaaaggc
     9421 attgccggtg ttttctcaga atcagcagct tgctgctgaa tcaataggtt accgacttgc
     9481 ggaccatttc cgtgggagat gatcaattca tctccttgtt cgataaattt cactaaatac
     9541 tttgctgttt ccattaacgc ttcttgttgg gcttttgcac ttgcatccgt agacaagatt
     9601 gcattacccc ctaaagcgac aactactttt cgtttggcca tttcattcgt cctttcttca
     9661 gttctaccga attttctaga taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc
     9721 tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta
     9781 cctaatgttg ctgccatgat cgctttgatt gagtgcatac gattttctgc ttggtcaaat
     9841 tgacgagcat atttgctgcg gaagacttca tctgttactt ccatttcagt gatgccaaag
     9901 cgttctttca tttgttcacc gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc
     9961 aagaagatca aacgatctgt gttgtgtgtt ttttctacca tttccatatt gatttggtaa
    10021 ggtttcaaca atttgatacg ttcttcgaat ttatcttctt cacccattga tacccataca
    10081 tcagaataaa ggacgtctgc tccgtctaca cctttatcaa catcgtcggt aatcatcaat
    10141 tgtgcaccgg atttttcagc aaagccttct gccattttga cgatttcttc ttcaggttgt
    10201 aattcttttg gtgcaacgat acgcatattc gctcctaaaa ttgcacctgt gactaataaa
    10261 gagttagcca tattgtttcg accatctccg cagtaagcaa cagtgatacc ttctactgtt
    10321 ccaaagtttt cttggatagt taaaaagtca gcgatcattt gtgttggatg ccattcatca
    10381 gtcaagccat tccaaactgg aacaccagag aattctgcta gttcctctac cattttttga
    10441 ctgaatccac ggaattcgat tccatcaaac atacgaccta gtactttagc tgtatcttct
    10501 gtggattctt ttttacctaa ttggatatca tttgcaccta gatattcagg atgtgcacct
    10561 agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa
    10621 agcgcaatat tttttccttc tagatagtga tgtgggatac cacgtttttt caagtctttc
    10681 aagtgttctg aaaaatcgat caaatattgt aattcttctt ttgtaaagtc tttttctgct
    10741 aataagcttc ttccttggaa tactgattct ttcatattaa acattctcct ttagattgtt
    10801 tttatttaag attaaagagg aaatcccgac ggcagctgac agtaagaccg ccgtcaggaa
    10861 acaaattact tcaagtcttc acggacaagt ggttggctca tgcagcgtgg accaccacgg
    10921 cctcgtgaca attcacttga attgatttct aacactttga tgccatagct acgaagcaat
    10981 tcgttcgata cataatttcg gttataagtg actacaacac ctggagcgat tgccaatgtg
    11041 tttgatccat cattccattg ttctcgaggt gcaacgatct catctccatt accagttggg
    11101 atcagtacaa gatcatctaa tccaagagca gctttcatcg tttcgtgtaa gttatctgaa
    11161 tgagtaatct tgatatcatc accatctggt tcgattgtaa agacatccat cttaccgtct
    11221 ttactttgaa ttgctggatg gatagtgaat ttatcgtgat ctaccattgt aaatacggta
    11281 tccaaatgca tcattgcacg gttgtttgga attttgattg ctaacacttt ttcaaatcct
    11341 gaattttttg cgaataggtg gcgtgctaat ttttctaaag ctttcgcatt agttcgttga
    11401 gaaatcccaa cagctactac tttatcgctt aagatcaatt cgtctccgcc ttcgatatga
    11461 tctggatttt cacgatccaa ccatacttcg attcctttat ttgcaaatct tggatgatgt
    11521 ttcaaaatat attctgtaaa catcgattca cgacgacgtg cttcaaaagt catcttattg
    11581 actgtcatcc catttcccat tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt
    11641 gggtccatgt aaaatggata gtcatcgtct gctgacagat cgtatagact gctagaacgt
    11701 acttcaatat cttttgtacg aaccccagcc atgattttat ctaccatttc ttgcgtttcc
    11761 attgatagaa ggaactcatg aagaccctct cttacagcat tcgatgcaat atgagattca
    11821 tccaacattt tattcaagaa ttgttctttg acattgcctg cgtcaatcgc ttctgccgct
    11881 aatttttcca agtaaagagt ctcgacgcct tcattttgca atgtctttgc aaagttgtca
    11941 tgttcttctt gagcaatcgg aagatatgga atatcatcga acaacagacg atccataata
    12001 tctggtgtca aattttccac ttcttgtcca ggccttttca gtaaaactgt ctttaatttc
    12061 cctatttcag agaaaacgtg aataggttta tccatgttgt gttgcctcct tctttttgat
    12121 tacatgtata ggatatcctc cttttgtaat cactttcata attttgtctc attca
//
LOCUS       DAZAID010000083        12164 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000083 DAZAID010000000
VERSION     DAZAID010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.085"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACG9BE_002187"
     CDS             <1..610
                     /locus_tag="ACG9BE_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3032919.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACG9BE_002188"
     CDS             723..1001
                     /locus_tag="ACG9BE_002188"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3032920.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACG9BE_002189"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACG9BE_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFN3032921.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACG9BE_002190"
     CDS             1458..2213
                     /locus_tag="ACG9BE_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN3032922.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACG9BE_002191"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACG9BE_002191"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFN3032923.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACG9BE_002192"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACG9BE_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFN3032924.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACG9BE_002193"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACG9BE_002193"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFN3032925.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACG9BE_002194"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACG9BE_002194"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFN3032926.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACG9BE_002195"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACG9BE_002195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFN3032927.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACG9BE_002196"
     CDS             6950..7492
                     /locus_tag="ACG9BE_002196"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFN3032928.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACG9BE_002197"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACG9BE_002197"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFN3032929.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACG9BE_002198"
     CDS             9085..9987
                     /locus_tag="ACG9BE_002198"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFN3032930.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACG9BE_002199"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACG9BE_002199"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFN3032931.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACG9BE_002200"
     CDS             11430..11852
                     /locus_tag="ACG9BE_002200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFN3032932.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4042 a         2158 c         2685 g         3279 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttatttgggc tctttgtcaa atgatgttgg taaa
//
LOCUS       DAZAID010000084        11738 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000084 DAZAID010000000
VERSION     DAZAID010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.086"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..160
                     /locus_tag="ACG9BE_002201"
     CDS             <1..160
                     /locus_tag="ACG9BE_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3032933.1"
                     /translation="LAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMPASEAAK
                     KDLIDILR"
     gene            561..1166
                     /gene="fic"
                     /locus_tag="ACG9BE_002202"
     CDS             561..1166
                     /gene="fic"
                     /locus_tag="ACG9BE_002202"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFN3032934.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1182..1754
                     /locus_tag="ACG9BE_002203"
     CDS             1182..1754
                     /locus_tag="ACG9BE_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3032935.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2006..2263)
                     /locus_tag="ACG9BE_002204"
     CDS             complement(2006..2263)
                     /locus_tag="ACG9BE_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032936.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2389..3204)
                     /locus_tag="ACG9BE_002205"
     CDS             complement(2389..3204)
                     /locus_tag="ACG9BE_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3032937.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3721..5046
                     /locus_tag="ACG9BE_002206"
     CDS             3721..5046
                     /locus_tag="ACG9BE_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN3032938.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5039..5389
                     /locus_tag="ACG9BE_002207"
     CDS             5039..5389
                     /locus_tag="ACG9BE_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032939.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5371..5556
                     /locus_tag="ACG9BE_002208"
     CDS             5371..5556
                     /locus_tag="ACG9BE_002208"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032940.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6460..6735)
                     /gene="prgO"
                     /locus_tag="ACG9BE_002209"
     CDS             complement(6460..6735)
                     /gene="prgO"
                     /locus_tag="ACG9BE_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFN3032941.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6707..7660)
                     /locus_tag="ACG9BE_002210"
     CDS             complement(6707..7660)
                     /locus_tag="ACG9BE_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3032942.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8272..9765
                     /locus_tag="ACG9BE_002211"
     CDS             8272..9765
                     /locus_tag="ACG9BE_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFN3032943.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9900..10196
                     /locus_tag="ACG9BE_002212"
     CDS             9900..10196
                     /locus_tag="ACG9BE_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFN3032944.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10297..11148
                     /locus_tag="ACG9BE_002213"
     CDS             10297..11148
                     /locus_tag="ACG9BE_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032945.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11169..11381
                     /locus_tag="ACG9BE_002214"
     CDS             11169..11381
                     /locus_tag="ACG9BE_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032946.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4036 a         1810 c         2175 g         3717 t
ORIGIN      
        1 tcttgcttat agtgttggtt ctttgataaa ggtaaacgtg tcacttgatc tatttactat
       61 tttactagcg gttggggtat cttcggttat tggactggtc ttttctgtaa tgcccgcttc
      121 agaggctgca aaaaaagatt taattgatat tcttagataa aatcagaatg acaagaatat
      181 ttacgttcta gattctaaaa taaaacacaa agagaccaac caggcaccct attgtttctt
      241 tgtagtatag agatattctt tggcttgtaa gagatcaata gagttaggtg accggagttc
      301 gaaataaagg aaatttttga ttgtatagga gaattcttta tccctaatat ttagaatttt
      361 gagtatgcca tgttgatgtt ttagattctc tataaatata gaggtgtatc aaaggtcaac
      421 tagactgtgc aaaatagaga cacttgaaaa atctatcggg ggtcagaaac ttaatagttt
      481 ttgacacata aacaaaatcg cgatataatg gggttgagag tgtttcataa acttatgtca
      541 taaagttaag gagatttcgc atgattttag agaataaatt gggattaacc aatcaagttg
      601 agttggctaa ggttgaagaa aaactcagta aacaaaaagc gaaagaattg tacgattgtg
      661 gaaaaataaa tgaaatcgaa gttggtacat ttaaaggttt atctgaaatt catgaatttc
      721 tattttcgga tatttacgat tttgccggaa aaatccgatc ggtcaatatt gcaaaaggaa
      781 attttcgatt tgcaccagtt atgtatttag aacattcgtt acagcatatt gatcagatgc
      841 cacaaacttc ttttgaagag attatcaaga aatatgtgga gatgaatatc gctcacccat
      901 ttcgagaagg aaatggccga agtactagaa tttggctgga tctcatttta aaggaagaat
      961 tgcaaaaagt tgtagattgg aacttaatcg ataaagcaga ctatctttct gcaatggagc
     1021 gaagtcccat aaacgatttg gaaatacgct atctaatttc caatgctctg actgataaaa
     1081 ttgacgatcg cgagttatat atgaaaggaa tcgatgtcag ctatttctat gaagggtatt
     1141 ctgagtatac gatagatgat ttgtaaaagt aggtggacca aatgacgaaa tatggatatg
     1201 cacgtgtctc aaccgcttct caaagtacaa aagaacaaat tcaacagttg cttgagaacg
     1261 gcgtagagaa gaagaatatt ttttcagaaa aatttacggg tacaaaacgg gatcggccaa
     1321 aattcaatca gctgttggct ggtctgaagc ctggagatga aatagttgtt acgaagcttg
     1381 atcgatttgc tcgtaacacg agagaagcat tagagatcat tgaaccttta ctcgatgata
     1441 acgtaacgat caaggttttg aatctaggaa cgattgaaaa tacgtccatg gggcgaatgg
     1501 tgacacggac gttgttgagt gtagcagaaa tggaacgtga tatgattgtg gaacgtaccc
     1561 aagaaggaaa aatgtttgca agaaagaata atcctaattt cagagaagga cgtccgaaag
     1621 caacaatcac accgaagaag aggcacgcat atgaactgct tacttcaggt aaatcgtata
     1681 aggaagttga agctataacc ggtttcagtc gcagtacgct ttttagaata aagaagaaaa
     1741 ttgaagaaag ttaaaaaaga agtctcagcg aaccagacag gtaaatactt aatgcgttct
     1801 agatatgagt ctattagcat gttgtgaaat ataatatttt tttatttact tgatttctgc
     1861 tcaaaaaatg tgtatggtaa ctgtaaaagg taaagtaatt aaaggtatgc tagccacgct
     1921 tactatatta ggataccatc atatatttat aatgaacgat ttgttcgtct accaaacaac
     1981 acatatcgtt cagcgtaagt cacgtttagg aacgtggctt ttttagtgtt attatcgata
     2041 aataatctat tgactctatt gattttatct ccatatccct acggtgttga ttgtctttag
     2101 atctttcatt taagaaccaa ataactatca taaaaatcag cactgctgct aatataataa
     2161 acagcattcc tattgtgata ttacctattg attttaccat aactacgatt aaagtaataa
     2221 tggcattcaa gtctcctttg ttctctatat ataaattagc cataaatcat cttcctttca
     2281 aattaagata cttgtttgat accaataaat agtaagcgtg gctagcatac ctttttctgt
     2341 aatagcaagt gttctaatag tccaaactta atgatggacc gatgctagtt atagtacagt
     2401 aatgttttct ttcttatcta gatttttagt aagctctatc agcaaatcta acaaatctga
     2461 gtccttttca gaaactaaat taaatctagt gtttacaaca aagttttcat gttctatatt
     2521 gtttagatta tttgcggaaa ggagggcttc tagatcttga cattcaaatc tatatgcatc
     2581 gtcataaatc ttactagaag gagtgcttaa ttcgaaatta cttattttct catcttggac
     2641 caagataaat ttatagatag tatctttttg tgtttttctt ttaaaaacac aacagagtgt
     2701 gttataagga acgttataat tttcatcagc ctcatttttt ctccattcaa acaaatgata
     2761 aactgcaacc aagttgccat caatttcatg gacagtgctc tcggtaacat cctttaaaga
     2821 actatcaagg gctaatttat agtaactaga gagaggaagt aaaattgtcg atttactgag
     2881 atggttatcg tctatacgtg caatcatacc taaatcaact aaggttttat tccaggtgtt
     2941 tatagtttta gttgtcactc ctagagactc tgctatggta ttgaccgaat gccaggaatc
     3001 cccgccttca ttctttgcct taaaacagta atacagatat aaattaatag ctttgttatt
     3061 cggtaaatat ttttcaagtt ctgttggtag aataaagtaa gaatccttaa tgttttttct
     3121 gaactgtctc cagtctctgt agttttgttt ataaagttgt gaaacggttt ttccaccttt
     3181 agaaagtcct gctatcttaa ccaatcttat tgctcctttc ttgaatctgt catgatcata
     3241 cagtattttt tacttgtagt ccagcacctt aatatattat cctatgtaat taatttcgta
     3301 ctagatagta attatttttt atttaattct ctagtcactt acaaataagt tatataaact
     3361 acgattaata aattattaca aaaactatta agaaaacttc tacatacaac tattacaaaa
     3421 ctattaagaa actgaatact cctatttttt gtttttaagg aaagtttttt cttttttgaa
     3481 tttcttattt ttttctaata taactaagca aaactggata atacgacact tattttcaaa
     3541 ttttcacaag actaatagta aatatccatt taataaagtg atacaatgtg ttgattttac
     3601 aaatcaacag aagaagtagg gaaataactt acctatgctt ctttttttgt acatttaatc
     3661 gattcttctt tacaaaaacg aacgtatgtt tgtataatgt ggaagaatgg agtgttattt
     3721 atgatggatt attcaaaaga gccagtaaat gattactttt tgatcgatat gaaaagcttt
     3781 tacgcaagtg tcgaatgtat agaaaggaat ttagatccat taacgactga attagtcgtc
     3841 atgagtcgag cggataatac aggatcaggg ttgattttag cttcttcacc agctgcaaag
     3901 aagaagtacg gtatttctaa tgtcagtcga ccacgagatc taccgcaacc ttttcctaaa
     3961 acgttacatg tcgttccacc acgcatgaat ctctatatta agcgaaatat gcaggtaaac
     4021 aatattttca gaaggtatgt agcagacgaa gatttattga tttactcgat cgatgaatcg
     4081 atcttaaaag tgaccagatc aatgaatctt tttacgactg aaggaacaag aagtcaacgg
     4141 agaaagaagc tggctcaaat gatccaggag aatattaaag aagagctagg attgcttgct
     4201 acagtaggtg tcggagataa tcccttatta gctaaacttg ctttagacaa tgaagcaaaa
     4261 cataatgatg gattccttgc tgaatggacg tatcaaaatg ttcctgataa agtatggaat
     4321 attcctgaaa tgacagacct ttggggtatt ggatctcgaa tgaaaaaaag actcaaccaa
     4381 attggtatca tgagtattaa agacttagct aattggaatc catacactat taagagccat
     4441 ttaggtgtga ttggcttaca gctgtatttt catgcgaacg ggatcgatcg aacagatata
     4501 gcgatcccac cagaaccgac aaaggaaaaa tcatatggta attcacaagt gttaccacga
     4561 gattatacca ggagaaatga aatcgaactg gtggtaaaag aaatggcgga acaagtagct
     4621 attcgtatca ggcaacataa ctgtaaaaca ggttgtgtcc atttatacat tggcacgtcg
     4681 atcttagaaa cgagaccagg tttttcacac cagatgaaga tccctataac agataataca
     4741 aaggaattgc agaattactg tcttttcttg tttgataagt attatgaagg acaagaagta
     4801 aggcacgttg gaataaccta tagcaagctt gtgtacactg attcgcttca gctggattta
     4861 tttagtgatc cacaaaagca aattgacgaa gaaaatttag ataaaattat tgacaagatt
     4921 cggcaaaaat atggttttac gtcgattgta catgcttctt ctatgttaga aggcgctcgt
     4981 agtattacta gaagtacatt ggttggtggc catgcaggcg gcgcaggagg gcttaaaaat
     5041 gattgattat aatgatcgtg gacgtttaaa gtgggcaggt ttttatttat ctgatcatac
     5101 tgaaaaaatt gatgccgaca atgaacaacg agccaatcaa aatttggcaa aagaacaaat
     5161 gacgactgaa gaggttgcgg aagtattgaa cgctgcagtc ataaaaaata gatcagtatg
     5221 tattcaaaaa gaagaacgaa acgctgaagg atactatcca gaagatattg ttagcaagat
     5281 cttaggtagt gatgagctgg ggatctacgt tggaaaaaac aaagttcact acgacgaaat
     5341 acgtcatatc gaattttacc aggataataa atggggtcga ttagaatgaa cattatccag
     5401 caatatgagc taaagtatat aacctttgat cagctatcag aagagatttg gggttatggc
     5461 cagcgtttga taaatgaggt tggtgttgaa cgcttctctt tctatgtaga agctgcagca
     5521 ggataccata aatacgaata ctatattggc tattaaagtg caataaaatt atatttcttt
     5581 tatgttttac attttgtatc aattctgata aaattaccta taggtaatta aaaaatataa
     5641 aaaaactagt tacaacctta ggacgttgta actagcttga agcatactag agctaactaa
     5701 aatagcacta gaaagcaatg ttaaacccgt ccaacaaact tgatttaaca ttgagaccag
     5761 cttattcagc taaatatagt tgaataagct tcttttgttt taaaaaaaca aaaggtgtac
     5821 tacgaataac gcaagtaaaa gaaatactat tacaaccagt aggctacaag taagtattac
     5881 taaacattgc gtagttagaa cctttgctct attgtctaaa gccacaaaat tagagatcag
     5941 ctttatgatg ctaagagcga gctgcatcca tgcggacata ggttcttttg acactattaa
     6001 ttctcctttt taggataagg gaaattgcta gtagtttttt cattagcacc atcccccttc
     6061 atttgattga gtctagtgct gctgtgatag tttgctgttt aatatgctta cattcttatt
     6121 ataacataaa tgaaacaaac acatggttag aggtcgtatc aatcaaaata gtgtctcaca
     6181 aacgatttat tacttactcc tgctgactaa cggactgttc ttcagtaact gtaactatat
     6241 gtttgttaaa accaccatta cctgtagata aaaaccaata tatttactat actcactata
     6301 ttaaatatat ttagtatagt aaaaactaga aacaataaaa tttgtgaatg ttgtataccc
     6361 ccaaacaata cacatatttt gaaggattag gattaataaa gctaaaaata tagtaagtat
     6421 actaaatata tttactatat ctagtatact tactatattt taatcttttg atcgtctatt
     6481 atatataatc cgttcctcgt cgtttaaagt atcaacatag gcgtccattg caagtttgac
     6541 tatttcatac ataggaatat cttttgcata actaagatcc cgaatggttt ttagtacggg
     6601 aggatctact tgaactggtt ttctttcttt cgcagtgtac tttcgttccc ctaactcgac
     6661 attaatatca tctttaattg aaatatcttc agcaccaatt ttcttcttat ctttgttcgt
     6721 tggtaataat gccatttagt ttaatctcct ttccaagagg taggatatga ttggccattg
     6781 agtcagcata tttaggtgta taatcaaatc cttcaatatc accatgttgt tcatagtatt
     6841 caattctttc ttctaattct gtaaagatgt ctgcaaaaat agcccaccat cttcgatcta
     6901 cataatcatt taattggata ccattctcac catacatttc taaccgatcc gagcctttaa
     6961 caatgctttt aaaaatgttt gattcaccat acttatcaat tgttttttga tagtttgcgt
     7021 gttgagagga acgtcgcact gagaaaagaa caggcaaaat tccaatgatt tgaagatgag
     7081 actcttcaaa attcgtgaca atcggaacta aaactttatt aataaaatct tctgttcctt
     7141 ccattgcgta ccgttttaat tcttgcattg caataatata atctgcagca gttaagaatg
     7201 cacgaaccac attatctgtt gatggtgggc agtcgataaa gatataatcg aaattttttc
     7261 tcaattcttc taaaggttta atgaaggcca atgatctctt acgacgatca ggatattttt
     7321 cggacaagta ttcattaagt tcttcagatc caggagagcc agccataata tataaatgtg
     7381 gacttacttg agtaatacct gaaacccagt cccctttttt gattgctgca gacatggatc
     7441 gatcaaagct agtaatttga tacgttgatc caatgttgga agtagtattt ctttgttggt
     7501 cataatcaat tagcaacacg tctttttcaa aaagttgact agaaacaatc gctaataaat
     7561 cattgtctgt agtcttacca atgccgcctt ttctacctgt aagcattaca acttttccta
     7621 gtttatctga tgttttaaat gcagtccgta agttttccat agttccctcc aatttcctat
     7681 actaaatata tttagtatac ttcatatact taatatattc gatatatcta atatactcca
     7741 aattgataag aattcagact ttataaggaa tatagtggat atactaaata tatttgctat
     7801 attcagtata tcagatatat tcaaaatgtc aaaatatatt tgtgcgtttt gcgttgacaa
     7861 tttagagtga aaagaggtaa acttaattct atagaaacca cagataattc actatttaca
     7921 aagtagcaaa agcagtggta ctctttaaac agaaaaatta cataaaaaaa agagacggaa
     7981 ataattgatt ttagcagaat caattatttc tgaatcacat ggttaggaca tgtgttcacg
     8041 ctcgtctcaa gaatatgtaa gtagctacca actacttata ttaaaagttg atcggatacc
     8101 ttttccgatt gcctttattc taccaaaaaa aggcgagcaa ctcaacagaa gaacgtttat
     8161 ttagtgtact taaattaaat attcttaaac actgaagaga gaacacatac tatggctgag
     8221 attaaagcta aactgtgttc tctttttgtt tgagaaagga cgttacaatc gatgttgagc
     8281 gaaatttatt cagctattct taaaaacggc ttacgtacat acaaatatcg aaacagcacc
     8341 atgaaacctg caggtcatat tgattccagt aggagtggtg ctatctttgc gtaccgtagc
     8401 aagcaattaa tgaatgttgg gcgtggtatg gtcattactt cggaagaagc tattttagag
     8461 aacgaaaaga aactaacgca ttggacacct aatacctatc gctttggtac ttatgcggat
     8521 gattatcgaa cagtggttaa aggtcattct gagaaaaact taagtcaaat caacacattt
     8581 gtagttgata tcgattcaaa agagaaccat caaggcgaaa ttattttagc ctgtttagat
     8641 caagtgggct acatgccaac gctaatttta gaatcagatc atggttatca agtttatttt
     8701 gtactaaaga caccaggcta tgtcacaaag aaaagcaatt tcaaggtgat cgatgtagca
     8761 aaaaaaattt caaaaacaat tcgtctacag ttggctgaaa aattgtccgg agtggatcta
     8821 ggatgtaacc acttcggaat tgctcgattc ccacaagaaa ataatattgt ttttaaagaa
     8881 atgaactatc agtattcttt tgctgactgg attcaatggt cgatgaagct tgaatcagat
     8941 caaaaaacaa aagaagatcg agaacgaaaa ctaattgtct ttcctgagaa aaaagaaatt
     9001 cgtcaagtag atgaaccgtg gttcgatatg ttacttcata aagcaaacat cgtcggcgga
     9061 gaaggacgtc ttggtcgaaa taatgtgatt ttcacgcttt ctttagctta ttactcttca
     9121 ggttatgcat tggatacttg cgaatacaat atgttcacat tcaacgaacg gttacaggaa
     9181 ccgctatctg aagcagaatt agtaaagatt gtacgtagtg cttattctgg taattatcaa
     9241 gcagcaagca gagaattcat tattgaattg tgccagcaat gggtttcacc aaccttaaaa
     9301 aataatgatc tgttcattca acgacgtggc tggtggaaat ttaaaaagcc acgagatcaa
     9361 agaaaatata gtcataaaaa tgaatggaaa gaagatctat tggcttatct aagcgaaaaa
     9421 agtcttgcta gtgagcctta tttgaacctg tcaaaaaaag aattggcttc acaactaacg
     9481 atgcccaagc gaacacttga taagatctta aatgagttac gagaggagaa taaagtcttc
     9541 tacaaggtaa cacgaggaag aaatggcgga atgatcattg cttcagtcaa aacgttgttc
     9601 gcgagggtta ttcaactaag taaagaaaag agactagatt attcaacaag gatcacagag
     9661 gtatttggac tcaacgagag cttagtagaa gagacattta gacaattatc aacccccttg
     9721 aaccctctta aagaagtgaa tctttttgaa gtagatacag gttgaaaaat aaaaggtgca
     9781 aattgcctta tacaatatat ctatgatact tctgtaggtg ttgctccctt tgcctagcta
     9841 cgtgtaggtg gtatacttat aactgcgtat gcagtcttat gtatcatgga ggaagttgta
     9901 tgtcgttaaa aaattatgtc tattcacatc ctgttaatgt gtttatcatt ggtcgtttgg
     9961 gattatctgt ggaagtgttc tgtgagcttt atggttttaa gcaggggact ttgtcttctt
    10021 gggttaccag ggaaaaaaca gttgcttctt tgccgattga gtttcttcat gggttgtcct
    10081 tagcttctgg ggagactatg gatcaggtat atgattgttt gtgtgtgtta gagcaggagt
    10141 atattgagta taaaatagct aacgagttaa gaaaaaggaa gaagtatatc cagtagtagt
    10201 ttttatttac ttaaaaaatt ggttcattga gcaacctatc gctgctaaag tagcaaagga
    10261 aaacgttaaa ggaaagaatt tagggaagaa atcgaaatga aatcggacga ctggattgaa
    10321 cacacctggc ttcctctgaa aagaaatagc aaacgaaaga aattttgggt attgcaatta
    10381 tggccattag gaaaactttg tagttacggg gcacgactta ttcttgattt aaccgtttac
    10441 tcgattggag tatttggaac ggcttattac ttgataaatg atcccaatag gattgcagca
    10501 tcagacattt ttacagtgct aatgacgttg atttttgtca tgctattgtc tatttttact
    10561 caattagtca tgcaatttac caggcaaaaa ttaacagcag gaaaactttt caaaagccta
    10621 ttcgatttaa ttgttgtaaa tgcaattcta gcgactttaa taaaacaaat tgaacaatct
    10681 ggaagaacaa caaaaggagt gaaggatttt ttattgacgt ttattcactt tgtaaatgga
    10741 cacatgtatt tgtttgaagt gggtattatt ctcttttttg tcgggtatgt atccacgaaa
    10801 cttattaaac gttcaattga aaaagaaatt gaaattgacc tgattccgac acttcttggt
    10861 tatgagtaca atgttgattt tggaagatat gacgaattga tcaatacgga tatcgttcac
    10921 acgagtatcc atgaatctac tgttaagggc atcagatcta ttttaaaaaa tggccaggaa
    10981 atgaaagcaa tagatcgaag aaaagagatc tacaattttt atgaagtcga agcaagccta
    11041 cgatatggca tcggaaaatt gtggttagat ggaagtaata ttcgtcaaaa caaaccatac
    11101 attttttgga acagtagtga tttagaactt ccaatgaaag ataaataatt atggagaatc
    11161 gtttgaaaat gattcctcta tacaggattg tgcttatagt tggcatggag ttaaaaacct
    11221 taaagaagga gcagttattc aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa
    11281 cgggtacgat gaacattgaa taccgaacag aatatttaga attaacgaat caaactgatt
    11341 ttttgttgat tcgaaagcaa caagagcaag aaaaaaatta atggctactt atagccaatt
    11401 gattgatgaa tgggaacaat tcaaggaatc taaaattcca gaacgtctct aaaagccttt
    11461 atttgtaaag agcataagga aacgtgggaa cactttttaa aaaaagtgtt ccaataggct
    11521 gtgcctatta cgtttctcaa aaataaatgt gggaacaaaa tatttaaaaa taggatagtg
    11581 aaaggatagt gaaggatagt aaaaggaggg tagtaggata gcaaaaagga taggctcctt
    11641 ttcgctatcc tttttaaatt ggaggaatta atatggaagt aagaataaga aatgtggatg
    11701 agaaaatcgt aagagaaatt gatcgaagat gtgacgaa
//
LOCUS       DAZAID010000085        11613 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000085 DAZAID010000000
VERSION     DAZAID010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.087"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACG9BE_002215"
     CDS             complement(105..629)
                     /locus_tag="ACG9BE_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFN3032947.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG9BE_002216"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG9BE_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFN3032948.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG9BE_002217"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG9BE_002217"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFN3032949.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACG9BE_002218"
     CDS             complement(2351..3031)
                     /locus_tag="ACG9BE_002218"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFN3032950.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACG9BE_002219"
     CDS             3157..4026
                     /locus_tag="ACG9BE_002219"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3032951.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACG9BE_002220"
     CDS             4052..4567
                     /locus_tag="ACG9BE_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFN3032952.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACG9BE_002221"
     CDS             complement(4614..5177)
                     /locus_tag="ACG9BE_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3032953.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACG9BE_002222"
     CDS             complement(5232..5411)
                     /locus_tag="ACG9BE_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032954.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACG9BE_002223"
     CDS             complement(5488..5997)
                     /locus_tag="ACG9BE_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032955.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACG9BE_002224"
     CDS             complement(6093..6473)
                     /locus_tag="ACG9BE_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3032956.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG9BE_002225"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG9BE_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFN3032957.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="ACG9BE_002226"
     CDS             complement(7736..9013)
                     /locus_tag="ACG9BE_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032958.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACG9BE_002227"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACG9BE_002227"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFN3032959.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="ACG9BE_002228"
     CDS             complement(9935..10879)
                     /locus_tag="ACG9BE_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFN3032960.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="ACG9BE_002229"
     CDS             complement(10881..11567)
                     /locus_tag="ACG9BE_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFN3032961.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3309 a         2462 c         2044 g         3798 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaataacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caa
//
LOCUS       DAZAID010000086        11563 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000086 DAZAID010000000
VERSION     DAZAID010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.088"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..120
                     /locus_tag="ACG9BE_002230"
     CDS             <1..120
                     /locus_tag="ACG9BE_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFN3032962.1"
                     /translation="PTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(735..1775)
                     /locus_tag="ACG9BE_002231"
     CDS             complement(735..1775)
                     /locus_tag="ACG9BE_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFN3032963.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2124..2450)
                     /locus_tag="ACG9BE_002232"
     CDS             complement(2124..2450)
                     /locus_tag="ACG9BE_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032964.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2437..3225)
                     /locus_tag="ACG9BE_002233"
     CDS             complement(2437..3225)
                     /locus_tag="ACG9BE_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3032965.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3593..3883)
                     /locus_tag="ACG9BE_002234"
     CDS             complement(3593..3883)
                     /locus_tag="ACG9BE_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFN3032966.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4019..4198)
                     /locus_tag="ACG9BE_002235"
     CDS             complement(4019..4198)
                     /locus_tag="ACG9BE_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032967.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4195..4545)
                     /locus_tag="ACG9BE_002236"
     CDS             complement(4195..4545)
                     /locus_tag="ACG9BE_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032968.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4538..5863)
                     /locus_tag="ACG9BE_002237"
     CDS             complement(4538..5863)
                     /locus_tag="ACG9BE_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN3032969.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6313..6927
                     /locus_tag="ACG9BE_002238"
     CDS             6313..6927
                     /locus_tag="ACG9BE_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3032970.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7092..7334)
                     /locus_tag="ACG9BE_002239"
                     /pseudo
     CDS             complement(7092..7334)
                     /locus_tag="ACG9BE_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7538..8857)
                     /locus_tag="ACG9BE_002240"
     CDS             complement(7538..8857)
                     /locus_tag="ACG9BE_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFN3032971.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(8991..9599)
                     /locus_tag="ACG9BE_002241"
     CDS             complement(8991..9599)
                     /locus_tag="ACG9BE_002241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032972.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
     gene            complement(10237..10494)
                     /locus_tag="ACG9BE_002242"
     CDS             complement(10237..10494)
                     /locus_tag="ACG9BE_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN3032973.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10487..10756)
                     /locus_tag="ACG9BE_002243"
     CDS             complement(10487..10756)
                     /locus_tag="ACG9BE_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN3032974.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(10887..11066)
                     /locus_tag="ACG9BE_002244"
     CDS             complement(10887..11066)
                     /locus_tag="ACG9BE_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032975.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            11164..11400
                     /locus_tag="ACG9BE_002245"
     CDS             11164..11400
                     /locus_tag="ACG9BE_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032976.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
BASE COUNT         3550 a         2091 c         1756 g         4166 t
ORIGIN      
        1 cccacgatta aaggcatgga agccatccga ggattatata agaaaacccg aaaagaaggc
       61 actctcttcg ggttttcggt ctgtactgaa atcaaggtat tattgggaat cccagattaa
      121 atcatagata ccgtaaggga ttttattctt tatttaaaac tttgcaacag aaccgtccgt
      181 tgcgggtaat accgtaacac tgaagggcac gcattaaggc gtcatcttaa tgctttcctg
      241 taggacactg acggagatta ccagtctccc gagccttaca ggggcctttc tttttttgta
      301 ttttcaatta tcacaattta tcgtccaaaa gtgcagaacg aaaatcaatt catcacaagc
      361 atatcagaca attaaattta aaacaactaa aaatatacaa aaaagtgtaa ttgaatatta
      421 gcagcactgc ctgaaaagaa gtaatatcag gcttttcagc ttggggaaat aagtgaatta
      481 cctctcttca tgaattatta taactcaaaa aagcacatat gcaaacacta gttccctatt
      541 tttttgaatg aaaaataact aaaatatcta acgattatga ctttaaaacc tacgtttgcg
      601 attgttttca taaactttta atctaaaaac tccggaataa tagctattta tttgtaacat
      661 ttgtaataaa attaaaatca taaaaaagtt ctaatttaaa attgatcatt tttatagtga
      721 tcgcttttta tcaactactc tagccaattt tccgtactaa ctgttggtaa ttcgttaaat
      781 gtttctcttt gttcgtttgc aagctttgcc gcaatagctt tttcgacaaa ccaatttttt
      841 acataaacga aaattagatt ctgcaaattg tttacttttt cagttttttg tttttgataa
      901 gctttcagca aagtcatata taatcctttg tcagcatcaa ttccataaga ttccagttct
      961 tcataaacaa caactgtttg ctctaattct tcagcttttt tctttgcatt atgaatagtt
     1021 tggacggttt tttgagcgtc ttcgtaagta ctagaaaatg tttttatcaa gtttaataca
     1081 ttttctggta caaatgtatt tatactgtcc tctttcatga tgtcgacgaa gttatctagt
     1141 aaaacttgat tttgtaactg atcagtttca gtgtctatta tgtgtctatt agtgtctata
     1201 gaattagaat agaatagatt gtctgcgatt ttcgcaatta cggttgagtc cttgatatga
     1261 ctggattttg ccgtatttgc gattttcgca attacggtgg tatcattgat ttctcgcggt
     1321 tttgccgtat ttgcgatttt cgcaattacg gtggtatcag tattgtcttc ctcttccatg
     1381 attttaaaga cgtcttgatt cgttacttct ggcttgccta agtacaataa attaggcatg
     1441 gattcaatct taccgtcacg tttatttact cttcctctct tctgtttcag aagacctgct
     1501 ttttctaatt cctttttgat tttagatact ttaccttcgc ggcattgtaa caatttcatt
     1561 aattcttcaa ctgtgaatat gaaaaatata ttatcatcag aatctaccca gttatttttg
     1621 actgaataat caaatcgatc tttaagtagc atataagcaa tttttgcatc atttgataga
     1681 tccatgtatt tttcgttagt aaaaaatacc ttaggcattt gataatattt ttcacgataa
     1741 atttcttttg atttgtaata attaaaatca ttcatagcca atcctctttc tgtaggatag
     1801 cgttttgttc aaaactatgt tacaatgaat taaacaaata tatctatcca ctatatattt
     1861 ttctactgct gataagtcgc caaactttgt taaagcagta gtcttttttt atcttttttt
     1921 agtttacctt aaaaaataaa ttaaagcaaa ccgctctatt tcaaggttta agctcatagt
     1981 aactatatca cgatattaca ataatattat atcacgatat cgtaatatta caatatgaag
     2041 ataagcgact tatcagcttt gatactactg atttttataa aaaaaatatc acgatattat
     2101 gatatcgtga tattgaatgt aaattaaatt ttattttgtt tctttgctag tttatatcct
     2161 tcaatcaagt attcttcagg tatggacttt aatagcgcat cttctataac gtctggttta
     2221 cttttgtctg attcagcaat agtcgtccaa tatttgagtg ctgacattag aattggattt
     2281 atacgaatgc tatctgctgt tcttacacta cttccttgat cttttatgac tttacttata
     2341 tttttcttgg ttaattcgat ttcttctact tcttttgatt taaaattctt tttattttta
     2401 tttcctagta atctacttac tgcagcttga ctgcgattat cttctgccat caaaatcacc
     2461 aacttctttt aaatattctt ctgtaaatgc tttatagtca tcttcagctg ttgagggttt
     2521 attaaaattg attagatctg atccagcagc ttgcgcttcg tcaacggtta ttgtcctacg
     2581 aatataagtt ttgaaaattt ttgcaccaat gactctagaa atttcggttg ctgcagacgt
     2641 catttcttta gaaatgttat aacgcttatt atacttagta aatagaactc ctcgtacttc
     2701 aagcttgtcg ttcgtatatt cttttaccgt ttcaatagtt tcacttaact ttattagtcc
     2761 ttttaatgag aaatacgcag cttcaactgg aatgataatt tcatcagaaa cagtaaatgc
     2821 gttgactgta agcattccta aacttggtgg acaatcaatt ataatataat catagttgtc
     2881 cttgattggt tgaagtactc ttttaagcct gtgttcactt cctacttgag taaattccct
     2941 ctctgcagaa gaaagggcta tgtcggctgg caaaacatca attcctttat agtccagaat
     3001 agcatctgta atgtcagcct ccccttttaa tacttcataa attgtaggtt ggtttgttcc
     3061 atctatatcg aaacctacat tatctgtagc atttccctga ggatcaatgt ccactaaaag
     3121 tacattttta ccgtaaacaa atttcaaaca tgctgctaaa ttaacgctag tcgtagtttt
     3181 agaaactcca cctttttgat tggctactga caatattctt gccattaaaa tatcccccat
     3241 tcatcttata ttataatata attatatcat aatattgtga tattgcaata tcgtatatgc
     3301 attatattgt aatattgtgt gattattttg ttttaaagat atatatcggt tcggatataa
     3361 agattattca tatgttattc gaaattaaaa taatcacttt ttattacttt aaaatttttt
     3421 attaatgata agaaatatgt tttgcgagca gcaactagaa taatttaaac attactaaac
     3481 tgtctatatt gttactaata attcccttct tatagtgcat tttcttagaa aaaaacaata
     3541 ttacgatatc atgatattaa gatatcgtaa tattatacaa ttgtgattga tattatatat
     3601 gtttttttat ttttttatca tgtttaatct tgaaggaatc atattctttt tctaaagaca
     3661 ggagcttttc ataaacatca gacatgttta aactagaggc caggcttaaa tcataaacga
     3721 atgaagcagg taatgatttt attctacgtt gtcgggttat ccagcttgca accgttcctt
     3781 gtgggtaggc atataactca caaaattgtt ctacagtgat tcctaaattc ttgataatgt
     3841 aaacattgat cgggtgcgag taaacaaatg tattgataga catggtgcgt aagcctactt
     3901 tcttttatta ttgcgtttgc aataatagtt taacacacgc attattctag tatctactcc
     3961 tatttgtaaa aacaaaaaag gattcataga gataactcta tagaatccgt aaaatacctt
     4021 atatatataa cgggaagata taaaacctaa aattatgata acctgctgca gcttctacat
     4081 agaaagagaa gcgctcaaca ccaacctcat ttatcaaacg ttggccataa ccccaaatct
     4141 cttctgatag ctgatcaaag gttatatact ttagctcata ttgctggata atgttcattc
     4201 taatcgactc catttattat cctggtaaaa ttcgatatga cgtatttcgt cgtagtgaat
     4261 tttgtctttt ccaacgtaga tccccagctc atcactacct aagatcttgc caatgatatc
     4321 ttctggatag tatccttcag cgtttcgttc ttctttttga atagatactg atctattttt
     4381 tatgactgca gcgttcaata cttccgcaat ctcttcagtc gtcatttgtt cttttgctaa
     4441 attttgactg gttcgttgtt cattgtcggc atcaatttct tcagtatgat cagataaata
     4501 aaaccctgcc cactttaaac gtccacgatc attgtaatca atcattttta attcccccat
     4561 ttccgcccgc atgaccacca acaagtgtgc ttctagtaat gcttctagcg ccttctaaca
     4621 ttgaagaagc atgtacaatc gacgtaaaac catatttttg ccgaatcttg tcaataattt
     4681 tatctaaatt ttcttcgtca atttgctttt gtggatcact aaataaatcc agctgaagcg
     4741 aatcggtgta cacaagcttg ctataggtta ttccaacatg ccttacttct tgtccttcat
     4801 aatacttatc aaacaagaaa agacaataat tctgcaattc ctttgtatta tctgtgatag
     4861 ggatcttcat ctggtgtgaa aaccctggtc tcgtttctaa gatcgacgtg ccaatgtgta
     4921 aatggacaca acctgtttta cagttatgct gcctaatgcg tatcgctact tgttccgcca
     4981 tttcttttac caccagctcg atttcatttc ttctagtata atctcgtggt aacacttgtg
     5041 aatttccata tgatttttcc tttgtcggtt ctggtgggat tgctatatct gttcgatcga
     5101 tcccattagc atggaagtac agctgtaagc caatcacacc tagtctattc ttaatcgtat
     5161 aaggattcca gttagcaaga tctctaatgc tcataatccc catctgattg agtctttttt
     5221 tcattcgaga gccaattccc caaaagtccg tcatttcagg aatattccat actttttcag
     5281 gaacgttttc gtacgtccat tccgcaacaa atccttcatt atgctttgct tcgttgtcta
     5341 aggctagttt agccaaaagg ggattatctc cgacacctac tgtagcaatc aatcctagct
     5401 cttctttaat attctcctgg atcatttgag ccagcttctt tctccgttga cttcgtgttc
     5461 cttcagtcgt aaaaagattc agtgatcggg tcacttttaa gattgattca tcgatcgagt
     5521 aaatcagtag atcttcatca gccacatatc ttctgaaaat attatttacc tgcatatttc
     5581 gcttgatata taggttcata cgtggtggaa caacgtgtag tgttttagga aatggttgtg
     5641 gtaagtcacg tggtctactc acatttgtaa taccataccg cttttttgct tcaggagaag
     5701 aagctaatat caatcctgaa ccagtattat cagctcgact cataacaaca agttctgttg
     5761 ttaatggatc taaatttctt tctatacatt cgacacttgc gtaaaagctt ttcatatcga
     5821 ttaaaaagta atcatttact ggctcttttg aataatccat cataaataac actccattct
     5881 ttcacattat acaaacatat gttcgatatt gtaaagaaga aatagctaaa tacataaaaa
     5941 aagaagcata ggtatgctat caccctgctt cttcttttga tttgtatgat caatttattc
     6001 tatcacttta ctaattaatt tgaaaatcta ataaaaactc tcactctaaa agagtgagag
     6061 actgcaaatt agagtacgga cttttttgga gttccgttct ttaatattaa caaatgaata
     6121 aaatccttac aacatttgag aataagttat cagtagacac ttacaaaaaa ttctcatact
     6181 atttcctcca tcattagttt ttgtctatat aaggtttacc ctaaatagac actggtataa
     6241 cattgaaaaa tatggcttcc tcgtgtatga ttagggtgta ttttaaatag ttgcagaaag
     6301 taggtaaaag ttttgagagt tgcatacata cgtgtctcat cgatcgatca aaatgaacaa
     6361 cgccagattg aagaaatgaa aaaattcggt gcagagagaa ttttcatcga gaaacaatct
     6421 ggcgcaacga ttactcatcg acctgttttc caagaagcat tagattacgt ccgagatcaa
     6481 gatattttta ttgtggaagc tattgatcga cttggaagaa attatgacga gatcatcgct
     6541 tcagtcaatt acttgaagaa gaaaaatgtg aagctgatca ttactagttt accgatcatg
     6601 gctgaagcaa ttggcaatcc tctcctggat aaattcatta aggatctgat catccaaatt
     6661 ttggccatga tcgctgaaca agaacggact gaatcgaaac gtcgccaggc acaaggaatt
     6721 aagatcgcaa aagcaaacgg cgtctacaaa gggcggccca aattatacag tgcagacgca
     6781 aaagatcccc aacgtcgcct tgtttacaaa agcatcgttg aagatcttaa aaatggtgtt
     6841 gcgattgcta agattgctaa agactacaat gttacgagac aaaccgtgta tagaataaaa
     6901 aaagacagca tggtcaatga caaatagttg atcatgctgc ttttattatc ttctcatctt
     6961 taatttccta catataatta ctatctataa aattaagtct tactcagaac agataaagca
     7021 taaaattcca acaaaccatt ttttacttaa ctaaataaag agtctgttaa tataatcttc
     7081 acttatttac gttaatcccc tactgtattt gttggttcct tatctatatt ttctacaaaa
     7141 tccttattat cttgtgattt agcttcgtat aattctttgt atgtcgacac attggttata
     7201 ctaattccta tagctagttc ttgagactct acttttctct ctagctcctt tgacaaagtg
     7261 atttgagatt tatttggttc cgttggagtc aattctatag attcttcata aaaatctcta
     7321 tacataaagc tatccctgaa taattcatag ctttaattct ttggcacatt ttattgaaat
     7381 atgtatcact aatatcccta tcaactgatt ttgactaaaa agttacttca aacattcact
     7441 gttcaaaaat attgaggaat aaattttggg tgtaaggcat tttgagcata cttctccctg
     7501 gataacttcc cagtaaaaaa atcgttagat tgttggatta gatccattga ctggctcgcc
     7561 tcaggcgagc aaagaccctg tagaattcat tctgatacac atgataacta gataatttga
     7621 gatagactcg tctacccgtt tggactaatt tcccagcaac tttcagaaac ttcaatcgaa
     7681 tagaatgaat ggtcattccc ttttggactt cttcaaagcc aatcgttctt aagaagttga
     7741 ctaagttgta agctatcaag ctgagcatca ttcggacatg attctccaaa aagcgaggac
     7801 tgtctgtttt gtcaaagtaa aagccagctt tcgcttcttt aatgaagttc tccattgtgc
     7861 cacgtttggc atagatagaa aagatggtat caggagaaac attttctgat agattcgtca
     7921 cgataaattc atgtcgaaag agtagttcgc ccgcttcacg tgttgagcgt atacacacgc
     7981 gacgactttg tgaccaggat tgtgcttgat aagtggtcga gaagtactga acttctcgtt
     8041 cttcccactt ttgattatcg ccataaagta ctgatttctc agctatttga cctagtctac
     8101 gattattctt cagtcgaata acataatgac tcttttttga ttcacacgaa tcatacacac
     8161 caggtgtagc gaacccgctg tctccacgaa ccaagatgtc agtgtttggt agagagtgat
     8221 tatagtgctc taataaaggt gtgagaaact cctttacgcc ttttgatgta tattgatttc
     8281 ctgaacgtag ttcagctttt aagaagtcac cagtcaatcc gtcaaaagct accaatggat
     8341 ggtaaccata ggtttggtag tgtgtattat aattcgtttg ttcttggtga ccaaatgtat
     8401 ctgaatgggt cgaatctaaa tcaatgatta attccgtatc attacggatg aggcgtgctt
     8461 tatcgataag ttcttggttc aaagcttgaa gttcatggat attctcctca gatagtcgat
     8521 ctaaaaatcg ggaaagtgaa gattgagaag cgagttcttt cctatctaaa acagctttaa
     8581 acacaggatc ttgtctcagg agattagctg cagaatcagc tgagtaacca gcaattaatt
     8641 gcataatgaa ctgctcaagt atggataagt tgtcatgagt aaaatacgct cgttcgtctt
     8701 caatgtgaat gtgttgctta gccagttcag aaaaaccaaa gatgttcatc agctctttca
     8761 ctaggacgag acccgaatca ctcgataatt gaccacctgt atgagaaata gtgatatttg
     8821 aattgaattt tacttggttt ttgtgtaagc tagtcatgaa aagaactcct ttcttttggt
     8881 tggtttagtc actttaacca tagcagaaag gggttctttt ttcatcactt aacgggtgaa
     8941 gatgaaaaag taattgtaag ctgccgtgag gcagtttcac atggttttaa ttaaaagtat
     9001 gaattattca ggctatcatt aattttataa aaaaaattaa atgtttttag cactgccata
     9061 taacattcaa aacattcttc tatagttggt ttacttcctt gaggagaatg aaccaattta
     9121 ttacgttttt ctatgaaatt tttaatttcc tttcttattt ctttcggaaa ctcaatatta
     9181 aagtaccctt taaacatgct ccctaataat ttgtctacag ggggagattg taaattctca
     9241 aatattagag ttaaatcagg tttctcttgt tgataaaatt cctttacccc tatttcacag
     9301 gctgtaatta tcgaaataaa cctatctcta gtatttgata tatttttact tcttttaagg
     9361 agtataagag aaaaggggat actttcgtgt ccatgcatgt aattaactaa ttgttgagct
     9421 tcagaagtta tatctctaat tatatagtta acggaattag gaagtgtctt taactctaat
     9481 gaaatttcta tccaattact atcttctaat ctgtatatgc tcgaagtata atttgattcg
     9541 tcagcaagca tcgctacatc ccaaacactg atcataaaat cttttaaacg ttcaatcatt
     9601 tcagcaatat tgctaaaatc agctaatttt tctttagaat aaaaaaaaac atttatatca
     9661 gaatcaataa attctatttt tttattacta tatcgtcatc attatcttga ccatttatgt
     9721 ttattgttcc ctgagtgggg agtacttctt tcaaattcaa gttaaattta tattccatta
     9781 cttctcctcc aatatataaa aattacttaa ctgtatccaa atttaatatg taaataaatc
     9841 attaattttt agctaaattc tatgagaagt ttatttttgt tgtttttgct aattaactaa
     9901 aactagtaca aaaaatttaa aatagattat taaacctatt gcttattcta ttcagtcata
     9961 tatataagcg tcatccttta gtaactaaca tcccacacta caatagtatt ataacatatc
    10021 tattcaactt caaattgttg aaatttaagc catttatatt tacttttcct caactttaga
    10081 aatgatttta ctaaccttat atccgtaata cgcgtaacaa acacattcat taataacaat
    10141 caacactaag ctatattagc tttttaaatg actagggtaa ataaaataag gatcaaaaac
    10201 ttttaagttt ctgacccttt ccttacttcc gattggttaa tagtgatctt ttgcagaata
    10261 aataaatatc gtttcatttt caactctata tatcagtcta tgttcatctg taattcttct
    10321 ggaccatttt ccagataaat catgctttaa tggctcaggt tttcctaatc cagcaaaggg
    10381 ggaacgatcg atatctttta ttaacttgtt aatctttttt atattgcttt tgtttccttg
    10441 ctcatgccaa taaagataat catcccaagc atcatcagac caagccttaa tcatcagatt
    10501 caacctcgat taagtcatgt gttttaaatg cacctttgga gaattgttca tctcctcgac
    10561 gaattttttc catgacgtaa ttattagaaa gtgttctcaa cgtttcttgc atagaatcat
    10621 aatctctttt tgataataca acaactgtat cttctacatc tttacttgtt acaataagtg
    10681 tttcagcatc ctcattaact tgtttcatat aactacgtaa attttggcgg aaatttgaat
    10741 aagctactgc ttccattcct ttcacccctt atttatttct ataaaacaat tgtacattat
    10801 attgtacaat taagcaaatg attaatattc taacttctta taattaaaag tgctatcact
    10861 tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc aaatcctgct
    10921 ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc acttttgccg
    10981 tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt aacgggtaac
    11041 cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa taaccctatg
    11101 aaaaatatgg ctgcaatcat cttgaatagt agactcacaa gggctaatac aaagcgccaa
    11161 aagatcaaac aaaataccag tattgccaaa aatactacga agtttaaatg cacagctgca
    11221 gcatggcgca taagtatttc gccaacaaat aggactaaca atagaggaat gattttattc
    11281 atatggatct ccttctttct cttaggttat cgggtgttgc ccgcttattt cgcaccacct
    11341 aagcggcggg ggcggttgga gcgattggtg cgaaataaga accattccct tagttactag
    11401 gttttttgtc cttatttcac acattctcac ttttcggtat aaatgattat tgcctttttt
    11461 tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg atttcttctg
    11521 tatcgtcgta agtataggtg tctaaaaaat cagttaaagt cgt
//
LOCUS       DAZAID010000087        11520 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000087 DAZAID010000000
VERSION     DAZAID010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.089"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            324..530
                     /locus_tag="ACG9BE_002246"
     CDS             324..530
                     /locus_tag="ACG9BE_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFN3032977.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(732..1733)
                     /locus_tag="ACG9BE_002247"
     CDS             complement(732..1733)
                     /locus_tag="ACG9BE_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3032978.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1738..3651)
                     /locus_tag="ACG9BE_002248"
     CDS             complement(1738..3651)
                     /locus_tag="ACG9BE_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN3032979.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3819..4325)
                     /locus_tag="ACG9BE_002249"
     CDS             complement(3819..4325)
                     /locus_tag="ACG9BE_002249"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFN3032980.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4485..4925
                     /locus_tag="ACG9BE_002250"
     CDS             4485..4925
                     /locus_tag="ACG9BE_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN3032981.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4951..5979)
                     /locus_tag="ACG9BE_002251"
     CDS             complement(4951..5979)
                     /locus_tag="ACG9BE_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032982.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(6034..6108)
                     /locus_tag="ACG9BE_002252"
                     /pseudo
     CDS             complement(6034..6108)
                     /locus_tag="ACG9BE_002252"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6111..6467)
                     /locus_tag="ACG9BE_002253"
     CDS             complement(6111..6467)
                     /locus_tag="ACG9BE_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032983.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6765..7739)
                     /gene="sgrA"
                     /locus_tag="ACG9BE_002254"
     CDS             complement(6765..7739)
                     /gene="sgrA"
                     /locus_tag="ACG9BE_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFN3032984.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7935..8774)
                     /locus_tag="ACG9BE_002255"
     CDS             complement(7935..8774)
                     /locus_tag="ACG9BE_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN3032985.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8959..9846
                     /locus_tag="ACG9BE_002256"
     CDS             8959..9846
                     /locus_tag="ACG9BE_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFN3032986.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10440..11134)
                     /locus_tag="ACG9BE_002257"
                     /pseudo
     CDS             complement(10440..11134)
                     /locus_tag="ACG9BE_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(11118..11516)
                     /locus_tag="ACG9BE_002258"
     CDS             complement(11118..11516)
                     /locus_tag="ACG9BE_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFN3032987.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3435 a         2295 c         1726 g         4064 t
ORIGIN      
        1 ggttatcatt tgtaatttta tttcacagtt gatatgattg ttatgtaaaa taaaaaagtt
       61 taggatacat cgaatttttg gggaaattct tttgggggta tcctaaatct tttctattat
      121 attcccatat tttctattat tggtctagac gtatgatggt aaactttgta cctatctgat
      181 tgctgaatca ttaattgctt atcattagcc atctttgttt caagttcgct agatgatcat
      241 tttattcaca tcctaacaaa cttgtcacat ttatagattc ttaatctatt tatcatacaa
      301 ttataaaaaa ggaggtgctg cttatgaaag ctatccatat ttctttgctt tttttcgcta
      361 gtgcattgat tttatttgct attcatcttt gttcccagcc ttatatcaat aacaacggaa
      421 tattagtgga accagcattc ttttgcttac cactcggctt tttgtccctt cttgcctccg
      481 caggcttcgg atttgttgct ttttttaaac gatcaaaaca acaaatctga cttataaaat
      541 ttacgtaaaa gagatcgtga caataatcgt gtgatattcc caaatttcga ataaaaggtg
      601 ccccttgcca tctctaactc ctcagtcaca agttacaata ttcataaacc atgaaaagct
      661 tgtttattga aattgctcct tgtgacttgg agctaagtga cagattacaa cctcttttgc
      721 atcataagac tttactcctt gaaaaacatt actaactttt gatatgtttc ttgcgcttgt
      781 ttattgctga aatcaaactg atattcatgg ttcaaaacag catttttctc cgcataatct
      841 acttttgtga ctttcacccc cgctttctca aatacagaag ttacttcttc catttgtttc
      901 gtaaacgtat ttgtatttcc atccgttaag aatgtgtttt gcgtccaacg atccgcatgt
      961 ctgaccaagg ctaaactttt gaccttctct gattgttcaa agtccttatt atcaaagtaa
     1021 gcccatccac atttttcata aagaaagcta gagattttat cacttacttg agtaagtgca
     1081 gaaggatcca acagaccaga caaagaaata aacttcgtaa tctgcttgtt ctccaaagtc
     1141 ggatcaagat gatcagcttt tttaatttct tcattttgaa gtgctaacaa atattctcca
     1201 ctaatttgtg cacctgccga atttccacca atcgaaatat tcgaccaatc tgcttgatct
     1261 tttatctctt ttttagctgc ttgaatggct tcgttcaatt ggataactgg tgtaggatgt
     1321 tttgcttccg gtgatcgttc ataatctata tttacgatga tttgctgcgt gtctgaacat
     1381 agatattcca aataatcacg acaatcattt ttatcccctc ctacatatgc gcctccatgc
     1441 atccagaaaa gaatcggtaa ttttttcttt tccactttcg gtgtgtagat atccatatta
     1501 ttatttttgt actttgaagg ataggatatt tcttttttta tatcgatttg ttcgattttt
     1561 tgctggaaat catctggtcg ggcatatact tgttcttttt cacttgtgtc aaattgtttt
     1621 ctaacaagca agctaatcgg ctttggtgtg atttgattaa tcaaaaatac agtgactaaa
     1681 ataactacga tgataactgg tactgcaatc actattcgct tgaaccattt cattcattta
     1741 ccctctcttt cttcgccaaa tccatgtgat aatcgtgcta acagataaaa atccacctga
     1801 ataaatgaga agcgaatgtt tcttttctcc agttttcggc aacattcttt tttttgcatc
     1861 tgtctttttt tgtactttca ctgtcatctg aatcggttgc gtatcatcac ttgattgatc
     1921 ctctcgaggt tcattctttc ccttattgtc tgcattctct gtcggatctt gcttttttgg
     1981 tggagctact gggatttttt tgtaatagaa aataatagtt ggtatcgtct cattaaaaat
     2041 cccttcagtc ggtccttcaa catttgctaa ttcgtatcct tcgaatgtct tttgctctgt
     2101 catataattt tctccataag ctccgtaaag ctggatttcg tcctggtctt tgagtagact
     2161 tttattgttt tcatccacat actttacgtt tatctgatca tggaacacta ctttcaaaaa
     2221 gtccgttaca tgtttcgtgt aggcagcatg gtcaacttcc tgtgcctttc catgccctgc
     2281 tccttctact aataaaagat ctttataccc tcctgataac tcatataatt tttgcgcgtt
     2341 agacacaggt actacatcat ctgctgttcc atggataatc aatttaggca atgaatctga
     2401 cattccaatc gtaggtagag cagcttttgt atccaaatgc atctcctgat caaaatagta
     2461 ttgtttcaaa aggcccataa ataaatcttc atgctcttga tccccaatga tgtctaactg
     2521 attttttatg gcatccggta gtgcgggggc tactgtctgg ctgtacgtaa aacgtagttg
     2581 ttcatcaatt gatgcaaatc cgcaattttc aataattcct ttgacattga caggaatatc
     2641 tttcgctaat acagacatca tcgtagctgc tcccatcgaa ccaccataaa gtaaaatctt
     2701 ttgagatggc cgctctcgaa cttcttgatt tatccagtaa aggacgtcat ctgactcata
     2761 ttgtccatag gtcacataat cgcctccgct attaccagtc gctctggaat caacaaataa
     2821 taaattatac ccggccttat aaaaatcatt gtattcttct gtaacgatcc cgttattcca
     2881 attccctcga aatccgccat ggatcatgac tgttttatct gaaccttgat cgacataata
     2941 tgcatataca tccatcattt tgccatcttg tttgtttagt acagtttgat gaagttgttt
     3001 tgtatttgga tagaagcctg tgttttctgt aggcgttgtc ccatcatctg tacctccaag
     3061 catcgacatc aacacactgt tttttgcaaa agcgatatcg tacaatagat aagtagcaat
     3121 cagatgtttg ataatctctt tatctacacc ataatcatta acagcaagct cagtgactaa
     3181 taactctatt aattttgact tgatgtcttc tgttggtcca ttaagtattt ctgtcatctg
     3241 tttaccgaag taattcaaat attctttacc attgaaattt tcacctttac cgatatcatc
     3301 attttctgtc ccattctctt tgtcacttac tttagcagaa gaggattcgt cgtttgacat
     3361 ttttgtagct gttgcttctt ctgtttgttg gttatctgtg gtagcggtcg tttcttgatc
     3421 tgtgctagta acagatgtct caaccgttgt ttcagtattt gtttctgttt gtgaagtact
     3481 cgatttcgtt tcatttccag catcagtagt ttctgtggtt tctacctccc gagattccga
     3541 ttcggaagaa atgctctctc catatactgt ccattgcggg acgcttgtac ttacagcaaa
     3601 taagatgacc aacgctccct ttaatacttg ttttgttctc aagctattca ctccttttta
     3661 tgtttctagg agaattatat gtaaacgcaa acatttttta tacaaaaaat aaatggttta
     3721 tcccataaaa atagaacagg attattttct cattttacgt aataaagcag aatggataaa
     3781 ccagtatcca ttctgctttt tttaaacgaa gaaacaactt attgctccaa aaatgtcaga
     3841 aatcgtttat cccaaatttt ctcgtaaaaa gcgattgttt cctttgcttt cataaaagga
     3901 ttatcataag ttgtcgttgc tcctctacac atcataagct ggtatcctaa tccatgagca
     3961 aacttcacag tcgtatctat acaatactct gcttgtgccc cacataattc gatcgaatga
     4021 cagtccaatt cagttaataa aacactcaaa ttagtcttat aaaatgaatt cgcatgggtt
     4081 tttctcacaa aataactgtg ctcaggaata gataattccg acaatagctc ccatggtttt
     4141 gtttgtggca ctaattgagc atcttcgtgt tgaacgaaaa taactggttt cccaatcgtt
     4201 tcgtatgttt tgattctttt attcactaat gctgtcactg tttctaattc gtgtatcggc
     4261 gaagaatcaa aacatacccc tttttgtaaa tcgatcacta ctattgcgtc ggctgtgttt
     4321 gacatacttg tgccttcttt cttttttctt gatttaatag tatttcttat ctagctagtt
     4381 ccattatacg tgacaacatt ttcttatttt acacctctaa atggcacttt tcgactattt
     4441 cttcatgtta ttatttcata aatacttagc tattggagga atgtatgatg attaaattgt
     4501 atacaggttc tggctgctcg tcttcaagac gcgcaaaacg ttggcttatc gtccatgata
     4561 taccgtttat cgaacagaac ttaattcgtc aaccactaac caaaaatgaa tttatccact
     4621 tactaaaatt gacaaacaac ggcgtgaccg aaatgctctc tactcattct attagttata
     4681 aaaatcttgt tcacagaata gatgaactca gtttgagtga actatatatt gagattcgta
     4741 catccccttc tttattgaaa aaaccgatta tctacgacca atctgcaaaa cttttgactg
     4801 gatttagcga agataaaatc aggatgttcc tttctaaaac taaaagaaag caagagctca
     4861 acgaactctt gctttctggt catcttgtcg attttaaaga aaataatctt tttgcaatgt
     4921 cttaattttg gcataaacaa tctgcttttc ttaattaacg aaatccaatt tttccaatat
     4981 ggatctattt ttctcattcc tgatttttat gatttcagct ttcagacgcg cccaatcatt
     5041 ttttgtaggt tgtccattcc agtaaaatat ctgtgtattc gtttgatcat atgccttttt
     5101 gtcgatagga taatcgctaa tgacaatatc aggtaactga tcagcggatg cactatattt
     5161 tattgggcgt tcagtcatag ataatatcct tttctttgcc cataattcct gtgttttacc
     5221 atactttgat tgaatataga atatgacagg ttgttcgtgt tttataaaaa tatccctgac
     5281 taacatacag tattgaaaaa tcaaccgtct gttcttttca taataagtat taagctctgg
     5341 aattctgcga gctaccccac taaaaaactg ttgcatgatt ggatacatca ctggaaatat
     5401 atgctgaaaa tctctgtctt ctgctgattt tccaaaagca tccaccttct tttttgtccc
     5461 aaaaaccctt gaatacaaat ggatcattgc caaattgaca tatagataat ttttttctga
     5521 agttgtaaaa caaatcttga agtttttttc aaaattttct acaatagacg attcaatcca
     5581 gccccaactt tcttgtactt tatctggagt atccaacaaa tcttcttcca aataggtgtt
     5641 cattacacca aacataaaat aaaggtaagc ttgctctgtt ggagtgatgt gattcatcga
     5701 ccagaagttt gcttcaaaaa aaggctctag tatgtaatgc gtaaactctt cttttgttat
     5761 caatggatgc acaaatttgt tcatctcttc tgggacatac gtaatcgagt ggccttgttt
     5821 cattcgttgc aacgaaattc ttagtaggct gatccatttc tttctatcta tactacgcgt
     5881 atatggcgca tgtttcgcaa atttttctaa tagttgacgt ttatttttta tacttgataa
     5941 tatatcagaa tgattttcgt cgaatgactg ccaatacatc aagtgaaaaa agtatcggat
     6001 acggtattct tctccttcta tcggattacc ttttttcaag ctgcaagata aatgatagtt
     6061 tttcaaaata gtattaagtt ctcttaattt tctttgaatc gtacgcaaac ttatataatt
     6121 ttttgactaa gattttctaa ggattgataa gtacctttgt aaagtgaatc cagtaaaata
     6181 taaccaatcg ttccctctaa aagtatagaa taaactttac tcagtaaaaa ctgagaagaa
     6241 aactcgactt ttatcatctt gttttctgaa agaaaagata atttatattc tggatacctt
     6301 tcgcaaaacg attctgcttg ttgaatcacc gaattcatct tagccagggt gtagtttaga
     6361 tctaaagcga tatccttcac cacatataat ttctcgtctc tttcataaca atagttcaaa
     6421 aattgatata cttgcctttc attgatatca ataaattctt caaacaaagt acatactcct
     6481 ttttatctat tatttagact tattccgaca gctacatgaa taacgtacaa atagattcgt
     6541 tactgatttt tcagagatac taccatgcgc agtgtgcatt gtcaattatt cttgctctta
     6601 ttttccattt gtgttcctgt tatttcctgt ttctcaaata tgttagtaaa caaaaataga
     6661 tctggcaaag ctacaacttc gccaggcaat acagcactct cgaatgaaat attcactcaa
     6721 aaatctatcg ttttattcaa ttttagatct gttgctttta ttctttattt cactcgatgg
     6781 aagagagaac gaaaatagct tgctcctaca atcaaaatga gtaacacacc catccatgta
     6841 attagcttgt tcaaggttct actaccagtt tctggtaaat cgctttttct attatctgtt
     6901 tctttcgctt tttcttgatg atcaattcca cgagaaacaa aatcaggctg ttttagcaca
     6961 ctttttaggt tgctccctgc atttctcttt tgttccttag ttggtatgat aggtaccaaa
     7021 tcaagttcat tatttacttt tcctgtgctg ctttcgctgc tcgtgcttcc tgtgctactc
     7081 tcactactca tgcttcctgt gctactttca ctactcgtac tctccgtact gctttcgctg
     7141 ctcgtgcttc ctgtgctact ttcactactc gtactctccg tactgctttc gctgctcgtg
     7201 ttttctgtgc taatttcact gctcgtactc tctgtactgc tttcaccgct cgtgttttct
     7261 gtactgcttt cactacttgt gctatccgta ctgcttggcg gtccaacaac tgtccaactg
     7321 cttggtttca aattctccgt gatcgtcatc tgtccatcat aaaaaacaat caaatcaact
     7381 ttatctttac tcttgatctc atcaatcgat agttctgcag aaatcattat tgtatactca
     7441 ataccagcgg tccattgtac gtcttttaaa taaacttccc caataacttt ttctgaactt
     7501 ccattaccat cccaaccata tgttagaaca gcatgtacat cactagcatt cacgcttttg
     7561 tttggtttga ctggaatagt aatgacttgt tgtgtcatgt cataaacaat ccgatcagtt
     7621 ttcacagtaa atgaaagatt ctcatttgcc cgttcatttg cagaagtact gattccaatc
     7681 attgctgcaa ttatactcag cccaagaata acaaataata cggtcgctgt tttcttcatt
     7741 tctatcactc tccatataat taccttctat ttgataaagg aattatataa aatgaaaatg
     7801 atgagaacac gacagtcagt tgtgaaaatc taacaaaaga tgtcgtgtat tcgcttgaat
     7861 ttgtttactt tttcgtttaa tcaatgacag acttaaaaaa gcaagaccga tatcagcctt
     7921 gctttcttca tcttttattg ataaacagca gtgtatttct tcatccgctt tgttttatat
     7981 gcatagagta acaaaccaac aatcaagact aacaagaata atgtagttga tatagatgct
     8041 tcagccccaa agtttccccc agtaattatt gtattttgcg attggatcag agtggagaaa
     8101 aaaccttgtt cttgcgttcc gctgactggt atacccaaaa tagctccttg tgtccaattc
     8161 caagtaatat gagcaccaat agccgtaaat aaacttcctg tactgtagat aagtgcgctg
     8221 aaaaagcaac ctgctaaaaa gatattgaaa actgccagaa atgtcatgtt aggattcaat
     8281 ccgtgcaaaa gagcaaataa tgcagcagat ccaatcattg ctattaacgg accaaatcta
     8341 agcatctgat gctgaaaaaa gccacggaat aaaagctctt ctgatgcccc cactgcaata
     8401 aataaaataa aactcgttat cagcggcaca atgtttgcgt ttcctgaatc taaccgatac
     8461 gcccccgtca aatataatgg actagtaata agcagaaaca ctccaattcc aacaacgaga
     8521 cctcccgcta aataccccca gtcttttcct cgaacagaaa caccataatc ttctattctt
     8581 tttccttcga ccttactagc aagaaataaa gctagtaaaa aaacagctaa atgtccgacc
     8641 gtaccggcaa ttgctccaat ccattcacta tttccaataa acatatccgc aagtgcatta
     8701 ttgacaagaa ttcctaaaac aaaaccaaca aagactatta aaacagatcc aatcaaccga
     8761 ataatcctga tcatctttca tccctccttt atattaagtt taacaaccct tttctttctt
     8821 gtctcgtgaa aactatgtaa atgttctcca tcatagagag aatagatttt tacgaactaa
     8881 gaatatctac agcaacctta cgaaaatgaa agactgtctc cctaattctt ctgttagact
     8941 gtttttaata aggaaaggat gggaaatttt ttgaaaaaac aactactaat tatgtgcctt
     9001 tcagcatgtg caattttact attctcaggt tgtagcaagt ccaatgaaac gattgcaacg
     9061 tttaaaggtg gaacacttac agttcaggat ttttatgata acccaaaagt taaatcaaat
     9121 aatcaaacac tattaaaaaa aatgattgtc ttcaaagcat ttgatgatgt atacggaaaa
     9181 gagatttctt caaaggaagt aacaaaagaa tataatgaac aaatcaaaaa actcggtcca
     9241 aactacaagg aacaattaaa agcagttggc caaacagaag agacatataa gaaactcttt
     9301 aaacagatgc tggctttcca atacggtttg aaagccaatg taaaattaac agataaagat
     9361 ctagacactg cttggaaaga attctatcca gaagttagta cacagatcat tctattttcg
     9421 actaaagaag aagcagataa agcaaaaaaa gaagcaaatg aaggagaaaa tttctccaag
     9481 cttgttcaag cttatggaaa aaataaaaca ttaaaggaaa ctgatggaaa aatgaatttt
     9541 gattcaacca atccagagat tcctactgaa gtaaaaaaag cagcatttaa actaaaaaac
     9601 ggggaagttt ctgatatcat tcctgtaaca gatccaacga cttatcagca aagctattat
     9661 ctagtaaaaa tggtaaaaaa acaggataaa ggttctaata aagacaaata caaatctgag
     9721 ctagaaaaaa ttgctaccga agcaaaaact cttgatacgg aattcatgga taaaacaatc
     9781 cgtaaagtga tgaaaaaaga caatgtaact attaaagatc cttatgtaaa aaatatattc
     9841 aaataaacaa atttgaccca attggggatg tggcaaaatt tgagaagtga tttttggaaa
     9901 ttccttctta tttttgaggc aaaccacttt tgccacaact tcatcggggc ctttttttat
     9961 ttgtaccaaa acaaatcact ggatttgtta caatcatgag gatataattt aaaaaagttt
    10021 tttatttcta tcacatgaat caatgcgcca ttaagtagat tctttcatgc ttccctattt
    10081 tttcacttgc cgtatcgctt caaaaacaac tatcataaac tcaaactcga ctgaaaagtt
    10141 attttaaaaa agactaaaaa tatcccttct ggacttgttc ttcttattct gttatactag
    10201 agaaacacga aagatatttt tatgcgacac ttttcatttg tttttatgaa atttattcaa
    10261 aactggcgta acttcagagt tacaaggatg tattagaggt tagggaatac atcgtttaaa
    10321 tgagaaataa gtaatagtta gtaatagtca cccaaacttt aaattaatga atattgaatc
    10381 gtcagatagc ctcctcgagt tttcgagaag gctatcgttt attttatgga gaaattgatt
    10441 cagaagtaat cagcgacctt ttttagttca ccaatcgttt ctgcttggaa agtttgcacc
    10501 caatcttctt ggaagcccct cactgcttta tcaatcgagg gcatctctaa tacttgatct
    10561 agtagctctt tacctaacgt tatcgctgag ctccagatcg aatggctgta gtgacttgtt
    10621 cacaattttt gaaactagct gctaatattt ttgttggtct ttgcgtacgt tcgatctcag
    10681 tccgcaacga atcaatcact ttcatagcat caatgccttg attttgcatc ctgttataat
    10741 aaggtgcaat ataatctgct ccattattaa tagctaaata cccttgtaat tctgtgtaaa
    10801 tagctgtacc ggtaatcaaa taaccttctt ttttcaactt tttgatcgct tgaaggcctg
    10861 cttgattcac cggcaccttg ataaacgtat cttttcccag ttcttctatg ataactcgag
    10921 catcctctat catttcttct tccgttcttc ctattgtctg aatatggata ggaatctgtc
    10981 cgattatttt tttgatttct ttcatatgcg agaagaagtt tgacgctcct tcttttttca
    11041 gaattgtagg atttgttgtt acgcctgtta aattcaaaaa ctgttgacaa gaataaatac
    11101 tttttgtatt tgctgtatca agcatgattt ccatatagct ttcccccatt ctgatcctct
    11161 tgatcgattt taatgattgt cccctttctt aaatgcggca tttccttatc ttctacatac
    11221 attgttcctg gaagttccgg ctcatgagat ccatcaaatt ttatcgtgat atgtcctaat
    11281 tcattcaaat tagtttggac aacatctcct actgctgtga ttacatacat ttgtttatca
    11341 aaacagagct tcatatcaca agttatttct tttcttacct gattcaattc aatcttataa
    11401 caataatctg ttaatgtttc cggtgcttca tctccaaata aaatcattat tttttctttc
    11461 ctgaatactt ctgcttctat accaacatct gttacaaccg tttcaaagat cgtcatctct
//
LOCUS       DAZAID010000088        11298 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000088 DAZAID010000000
VERSION     DAZAID010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.090"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            452..721
                     /gene="rpsN"
                     /locus_tag="ACG9BE_002259"
     CDS             452..721
                     /gene="rpsN"
                     /locus_tag="ACG9BE_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFN3032988.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            736..915
                     /gene="rpmF"
                     /locus_tag="ACG9BE_002260"
     CDS             736..915
                     /gene="rpmF"
                     /locus_tag="ACG9BE_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFN3032989.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            947..1324
                     /locus_tag="ACG9BE_002261"
     CDS             947..1324
                     /locus_tag="ACG9BE_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032990.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1345..1494
                     /gene="rpmG"
                     /locus_tag="ACG9BE_002262"
     CDS             1345..1494
                     /gene="rpmG"
                     /locus_tag="ACG9BE_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN3032991.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1518..2501
                     /locus_tag="ACG9BE_002263"
     CDS             1518..2501
                     /locus_tag="ACG9BE_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN3032992.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2531..2653
                     /locus_tag="ACG9BE_002264"
     CDS             2531..2653
                     /locus_tag="ACG9BE_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFN3032993.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2671..4194
                     /locus_tag="ACG9BE_002265"
     CDS             2671..4194
                     /locus_tag="ACG9BE_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFN3032994.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4218..4457
                     /locus_tag="ACG9BE_002266"
     CDS             4218..4457
                     /locus_tag="ACG9BE_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3032995.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4650..5111)
                     /locus_tag="ACG9BE_002267"
     CDS             complement(4650..5111)
                     /locus_tag="ACG9BE_002267"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN3032996.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5306..5524)
                     /locus_tag="ACG9BE_002268"
     CDS             complement(5306..5524)
                     /locus_tag="ACG9BE_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3032997.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5642..7258
                     /locus_tag="ACG9BE_002269"
     CDS             5642..7258
                     /locus_tag="ACG9BE_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN3032998.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7499..8839)
                     /locus_tag="ACG9BE_002270"
                     /pseudo
     CDS             complement(7499..8839)
                     /locus_tag="ACG9BE_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9277..9423)
                     /locus_tag="ACG9BE_002271"
     CDS             complement(9277..9423)
                     /locus_tag="ACG9BE_002271"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3032999.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9462..9656)
                     /locus_tag="ACG9BE_002272"
     CDS             complement(9462..9656)
                     /locus_tag="ACG9BE_002272"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN3033000.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9566..9898)
                     /locus_tag="ACG9BE_002273"
     CDS             complement(9566..9898)
                     /locus_tag="ACG9BE_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3033001.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10412..10603)
                     /locus_tag="ACG9BE_002274"
     CDS             complement(10412..10603)
                     /locus_tag="ACG9BE_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3033002.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10697..11083)
                     /locus_tag="ACG9BE_002275"
     CDS             complement(10697..11083)
                     /locus_tag="ACG9BE_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN3033003.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3767 a         1777 c         2173 g         3581 t
ORIGIN      
        1 attaaacagc atctaatttt gaatacacga aagaatcagt atgattgaat tagttcactt
       61 aatgtctatt cttataatat aaggaataat caaagtttta aaaaattggc gataaaccag
      121 ttaaacataa aagattgaat aaaaagttat agtttctttc atattgccgt tttaagtaga
      181 ggaaaagcta cttaaaacgg tatttgtgtt tgattggact aataaaagtg aattcttatt
      241 tttcagagcc taaactgcat attcttttaa atgatttgat tatctctatg taccgataaa
      301 ttcgttttca aatagttatc tattcatatg agttttattt catgttattt attgttgaca
      361 agagcaactc ttgttggtaa agtatcagtg tcaaaacgta acgattacga tttgaggtgt
      421 ctaaatcaaa ataaaaggag tataaaggta tatggcaaaa aagtcgaaaa ttgctaaagc
      481 aaagaaacaa atggcgatga ttgaaaagta tgcagataaa cgtcaggaat taaaagccgc
      541 aggagatcgc accgctctcg ctaaattgcc aagagattca aatccgaatc gtctgaggct
      601 tcgtgatcag acggatggtc gtccaagagg gtatatgcga aagttcggta tgtcccgtat
      661 taagtttaga gaacttgccc atcaagggtt gattcctgga gtaaaaaaag caagttggta
      721 aaaggaggaa atatcatggc agtaccagca agaaaaacat ctaaggcgaa gaaacggtta
      781 agaagaactc atcaaactgt agtaaaacca gaaatttcat ttgacgaagc gaatggtgat
      841 tatagacgta gccatcatgt atctttaaaa gggtactata atggaaagaa agtaattaaa
      901 ggagcttcaa aatagtttaa tatataactt ttaggaggga atcaaaatgg aaattattta
      961 cccaccttta gttgaagaag gattaaagta ttatcttgaa acgacccagc aatcgctaga
     1021 taaatgcact ttttatcgtt cgatggtaga gagaggaatt attactgaaa cgggtttacc
     1081 gacccaacag gcaattgaaa atggattagt caaagattac tatgaggacc aaggcttatc
     1141 atttgatgat tttttaagaa tctatccaat ttttgaagag tatgacgagg agttatttca
     1201 atgtatcgat ggttattggg agatacctgt tgatatgaaa gaaaatttag tttcacaatt
     1261 ggaatctggc gagttaactt ttgaagatgc acaacaaatt caagcatatc ttgaagatcg
     1321 ataagtattg aaggaggaag atttatgcgt caaaatatta ttttggaatg tgtcgaaact
     1381 ggagaacgtc tgtatctcac cagcaaaaat aaacggaata accctgaacg gttagaatta
     1441 aaaaagtatt cgccaaaatt gcgcagacgg gcaattttta aagaagtgaa atagctaagt
     1501 ggtaaaaagg tgacaaaatg ggcataccag ttacaatcgt ttctgggttt ttaggggctg
     1561 gaaaaacaac gctaatcaat caagccttgc aagtctcacc ctaccccagg gaagaaatta
     1621 ttataattga aaatgagttt ggtgaagttg ggattgatca caaattatta gttcatagcg
     1681 aggaacgggt gttacaacta aataatggat gtatgtgttg cagtcttcgc agtgatttat
     1741 tagcaatatt gatttcttta ctggaaacaa tagaagaaca tcaacaacca atttcacaaa
     1801 tcatcattga aactacaggt atagcagatc cccaaccaat cattcaaaca cttttaacag
     1861 cgcctcaact aagaggaaga gtatatatcg atagtttatt gactgttatt gatagtgaca
     1921 gttttgaact tattgaaaga gaaccacaag cccttaagca acttgtcatg gcagatcgca
     1981 tttttgtgtc tagaaaacaa aacttgaatt tatctgcact aaaaaaatta cagagagaaa
     2041 tcaaaagtat taacccttta tccgatattc gaatgttcac acatagtgaa acgattcaaa
     2101 aaaaggattt ttataatttg aaaaaattta atcatccctt agtttttgaa agtgattcat
     2161 cagaaaatca ctataatggc aaatactatc atcaacacca tcaagtacat gagtttaaat
     2221 caattctctt aacttcagat caagacataa aaaaagattt tttactccaa tggattgact
     2281 ggctgatttt aaccaaccaa gggagcctgt atcgttttaa aggattgatt gctttacaag
     2341 agcaggattt tatagttgcc atgcaagagg taaacgaaca ggttaatttt caatacacca
     2401 ctcgacaaaa agaccaaacc actattattc taatcggaaa aatgttggat gaagaaagga
     2461 tcaaacagag ttttgataaa ttactggcgg aaagcaaata atgataagta gtgattgggg
     2521 gtaatttcta atggagaaaa cggatatatc tagtgcatat cgacgactta aaagcccgaa
     2581 tattaaaact agaaaaaggg cattaaaaat tatcaaagaa cataaacgaa ataaaatgaa
     2641 gaaattagca taattaagga aggaaaaaga atgaagaaaa tcagtgtagg acttattggg
     2701 atagcagctc taggattatt gggagcttgc tccagcacta atgatgcaaa agtatcgaat
     2761 gataaagatg gaaaattaga aattgtaaca acattctatc caatgtatga tttcaccaaa
     2821 aacatcgtcg gcgatgaagc gaacgtggat ttaatggttc cagcgggatc agaacctcat
     2881 gactatgagc cctcagctaa ggatatggca aaagctcatg atgctgatgt ttttgtatat
     2941 cataacgaaa acatggaatc atgggtacca aaagctaaag aaagttggaa gaaagctggg
     3001 cctaatgtag tagaaggaac aaaagacatg attttattgc caggtagtga agaagaagat
     3061 catgatcacg gagaagaaga tcaccatcat gaactagatc ctcatacgtg ggtttcaccc
     3121 aaaatggcaa ttaaggaagt ttcaaatatc aaggatcagt tagttaaatt gtatccgaaa
     3181 aaagcaaaag tatttgagac aaatgctgaa aagtatttga ctaaactgaa acggttggat
     3241 gctgactata ccactagtct taaagaagcg aaacaaaaaa gttttgtgac gcaacacgca
     3301 gcctttggat atttagcttt ggattatgga ttgaaacaag ttcctattgc tggactaagt
     3361 cctgaagaag agccatcttc tggtcgatta gctgaattaa aagagtatgt taaaaaaaat
     3421 aagatcaatt atatttactt tgaaaagaat gctaatgata aaattgcaaa aactttggcc
     3481 aacgaagctg ggataaaatt ggaagtactg aatcctcttg aaagtcttac aaaagaacaa
     3541 atggataatg gtgaagatta tgtttcagta atggaagaca atctaaaagc gttagaaaaa
     3601 acaactatgg ttgctggtga agaggtagtt ccagaaaaag aagcgaagga tgaaaagaca
     3661 gttgctaatg ggtactttaa agatgccgat gtgaaggatc gcgaattgag tgattataca
     3721 ggcgaatggc aatctgttta tccgttgtta aaagatggaa ttttggatga agttttcgat
     3781 tacaaagcaa aaatcaataa agacatgaca gctgccgagt acaaagatta ttatactact
     3841 ggctataaga cagatattga tactatcaac attaaagaca acaccattga ttttgtagtc
     3901 aacggtgaac atcatcaata cacatacaaa tatgttggat acaaaatttt gaattatgaa
     3961 aaaggaaacc gtggtgttcg ttttaatttt gaaactgatg atgcaggtgc tggtcgtttt
     4021 aaatatattc aatttagtga tcatggaatt gcaccaagta aggcagagca tttccatatt
     4081 ttctttggtg gagaaagtca agaaaaactc tataatgaat tgcataattg gcctactttc
     4141 tatccagctt cattgtctaa acatgaaatt gctcaagaaa tgatggctca ttaaaattca
     4201 atttttggag gaaatacatg gtaagaaaag tattattgtc tatggattcc aagaatattg
     4261 atgcttatcg gcgttatcga tttgcaaagc aaatgagatt aaatgggagt acattagctg
     4321 atattgctgc aactttacat gttagtattg acacagttca tagagtatta aaacgggatc
     4381 ctgattgttg ttttatcaag gtcaatactg gaagaagtag acgtttagca aaacggcata
     4441 atagcttggc caattaattg ataataccag agtagatcat ttttccctta tttggatttg
     4501 atctgctttt tattatgtct tgaattgtca aatcaagcgc aacactttat caaagaagac
     4561 ttcataaggt gttctccaat ctaaacactt tctaggtctg ttattcagtt tttcaatcat
     4621 ctgatttatc gttgagttat ctaccgtagc tatctctttc gattttggca agtattctcg
     4681 aatgagtcta ttcgtatttt aatttgttca ttgttgccaa gggcatgagg atctggaaaa
     4741 taaaagggca tattattcaa tgcttctgta actgcgaaat gattggagaa ttcttttcct
     4801 ctatctggag tgatcgtttt gaccgcctca tttggaatac tgcttaaaag ttcaatcatt
     4861 ttctcgctca cataagcggc tttctttctg gggaccttct ttcctgctaa aaggaagcat
     4921 gattttctgt ctgtcaaagt cactaaacaa gtaccaccag ttttgccagc tacaatatcg
     4981 gctttccaat gaccaaaatc acttctatca tctgcggatt ttggacggcc atggatcgaa
     5041 tgagtaaggc aaatctttcc acgagactcg acatgatttt ttgtatgtct tgtttttcct
     5101 cgatggtgca atgaacggat caggccttta ttgccgtgtg agaggggttc tgtatcgaaa
     5161 agatcattgc aaattgcacg ttagatcgtg ataaaactaa ttgaaaagtc catatgttca
     5221 tgcttcaaac gattcgagat ttcctctggc gaccactgaa aatcgacaaa taaatgacga
     5281 acaattgacc aaactcacgg atcatttaga atgtgataac gtccgcattt ttttcttcga
     5341 taagcataat ttttttccgc tttggaaggt gaatagtttt tttcttgttt cttctcagtt
     5401 catgtaaaat agtaaacggg ctatgctgaa tgtcgttgcc aattatacta atagaaaatc
     5461 cttcttcgtg aagcagaaag attctttctc gttcgagtat agtaagatga cgatactggc
     5521 tcataattat tttccttcta ggagtgttct cgacaaactt atactagttg gatatcgatt
     5581 atgactcatt tttatgtgtt gcacttaaag tgtaaattca aggtatagaa agaggttccc
     5641 tatgaaaaaa atacctatta caatcttaac tggctatctt ggttcaggaa aaacgatact
     5701 tctaaatcat attctgtacg gtgatcacgg aaagaaaatt gctgtcattg taaatgacat
     5761 aggggaagta aatgttgatg gggtattagt cgacaagaaa ggttttaaaa gaactggaga
     5821 aaaacttgtt tcgatgccga atggatgcat ttgttgtaca ttacgcgaag atttgattgt
     5881 tgaactagat caattagtgc aattttctga cttgaatcaa attgttattg aagcttcagg
     5941 aatcagcaaa ccaattccaa ttattcaagc aattatatta gctaaaaacc ctttagacat
     6001 tgatttgacc caacgattag agattggtgc cattattata gtagtggatg taaaacgttt
     6061 ttgggataat ttcgaaacgg gtgaaaagat tcaggaaaga aaagctgaag gtaatgtagt
     6121 tgaagaagaa caggatattc gtggcttgtt aattgatcaa ttcgagtgtt gtaacatatt
     6181 acttttaaat aatagtgact tagtgtcaac caagacgatt tacaaaatta acggtctaat
     6241 tgctaagtta cagtccaatg ccaaaataat taggacaatt aaaggtgaag tagacttatc
     6301 gaaaattcta aatacaaatc tttttaatat agatgagact tcctattcag tgggctggtt
     6361 caaagagtta gagcttggct atcaaaatca tattcctgaa acagaagaat atggtatctc
     6421 aagttttatc tatcgaaatc ggttactcct tgacgcacaa aaatttgccc atcttacgga
     6481 taaaaatttt cctggcaata ttacaagagc aaaagggttt ctttggtttg cccaacatag
     6541 tgatttttcg acgatctttg aaacatcggg tagctataaa agtattgaat cattaaatta
     6601 ttgggtagct agtttaccta aaattgagtg tcaaatgatt atacaaaaaa atcccgaatt
     6661 gaaggaagcc tggcatccgt tgtttggtga acgcattaat aaactagttt ttattggtat
     6721 gaagatggat caacaatgta ttaaggaaca acttgagggt tgtttaactg attctcttaa
     6781 tgcagataat ttccgaaaaa tttcaccatt tccatggaaa aagtctaata tgtttagcca
     6841 gatatcgtta atgaaatttt taagaaaagc tggtgtcaag ataggcgatg tcataattat
     6901 tggcattttg attattacct cttttattcc cttatttgta ttctctatag aaaaggagaa
     6961 tgaaacaact gaagatgcta tcaaatatgc catcgttcga attgatggca aggaaatgga
     7021 tcgcttcgat ttggataaga tcaatcataa actcgtgaat tatcatcctg cgaagggaca
     7081 atataatatt gttgagatta aagatggaag aattcgagtt aaagaagata atagtcctga
     7141 tcaaatcgcg gttagaactg gatggatctc aaaacccggt caaacgagta tttgtttacc
     7201 ccataagtta gttattagta ttgagaaaaa agaaagcaca gattactaca tttactaatc
     7261 tggaaataga tgaggaggtt tttaataaga aaaagatatt attattaata atagcagggg
     7321 ctttattaag tgtgttaagc gcttgtggac aagaaaaaga agcggtgaca acaggcacaa
     7381 gtatctttaa cgggacctgc aatggtttag ttaacagtcg gttagattac ttattttggc
     7441 tcttcgtcaa tgagtgttgg tgaagaaatt caatagaata atcgagatac tcacacggct
     7501 agacagctat cttgtctagc cgtgttgttg ttctggcttt agtcagttgg gtagtattct
     7561 tagttttctt aaaatatttt cctcgatata aaaagattcg ttctcggtag ttttgaaagt
     7621 ttctatagcc ataggcaatt cgttttagta ctttgatatg gttattcaaa cattctatag
     7681 ggccgttgga atagggaacg cgcaacgctc gtttgatttc tgtttggtat ttcttgaagg
     7741 tcgtgaacac tggttggtag tgctctggca actccttgta gtcttctttt aggagcgcga
     7801 cgaattcagg aacatcttgt cttctgataa cggaaagaaa atcctggtag acttcgtagc
     7861 ctcgctttaa ggaatcatca taagataata gccgatcgac tatctctgct tctgtcagat
     7921 gatctcgaaa tgacggacgc cagtagcgat gctgaccttt caattcccac gcattctttt
     7981 gaagtagttt ccagtatttc tttaagtgct tcgaccgacg atcccctttt ttaaaggtct
     8041 tcattacttg aattcggtgg ttggtaaaag cccgaccaat gtgttggcta atatgaaagc
     8101 gatcggcgac gattcgggca ttcggaaaac actctttcac caatgtgcga tagggtgtat
     8161 agaaatctga aaccactaga cgcacttgtt cacggacttt tatagggtag cgcaagaaat
     8221 agtcgcgtaa aggattgagt tgccgatttt ccacaatatc aatcagttga tgggtcttgc
     8281 cgtccatcat gacaaagctc atggctcccg agacattttt cactgatttg aattcatcga
     8341 aacacaccac ttctggtaac agctggctca aagaaatggt tttaggccga aggctgcgaa
     8401 gtattcgaat gacagtggtc ggagaaatgt gcttcatacg tgcgatcaaa gacatagaaa
     8461 gaggttcgct taatagctca agaatggctt gtttgactct gcgagcaatg gagcagcgac
     8521 gatcactaac tgaatcttct gctaaaaaag tctttccaca gtgtttacat ttgaagcgct
     8581 gtttgttaat ccgcagaatg gtcttgtagt tactgacatc attcaataag atggatactt
     8641 ttttccagcc ctatttaatg atcgtttgtt cattttgata gtggcgatga aagcattcct
     8701 ccggttgata ggtcaatgtg ccatcaaaaa caaaacatgt ttcgtcattg attcgctctt
     8761 tgcgaaaggc atcttcatga aagataagat ttaggtctag aatatctagt aactctttgg
     8821 taaactctga ataggacatc ttgaaacact cctttgaatg gttttcgtcg actttattct
     8881 aaaggatttt aaggtgtctg tttgcgttta ttagaaaaaa gaaaaagtat tggcaacaga
     8941 aggcgtcacc aacactagat attatagatc ccttattttc ttcgcttcaa attgtcaagg
     9001 agtcatcata aattttgtat ggattctttt gtttgggtag tagtcttcta aataatcttg
     9061 gattaacacc ttcatctcta ttttactttt atatttgttg gagtcgatta attctcattt
     9121 gattgatcga tgaaacgctt caattactgc attgtcaaca ggagtcccag tttggctcac
     9181 tgaatgtgtg atgtggtggt cgttcaacag ctcttgataa ataaaagacc gatgctggct
     9241 acctctatcg gaatgaatta ccgtaggcaa ttctccttaa taaatttgta atggacggac
     9301 gacaatctct tttttcatgt gtgaatctac catatagtaa cgtagaatac gagttgctaa
     9361 gtcaatatag gtggacaaat agaggcgacc gtcattacat ggaatgtaag taatatccat
     9421 cacccaaaca gcatcaattt ggttctgttc aaattatcga ttcaacattt ttttaccatt
     9481 tctttgattg ttttttctct accatgagaa gatttgcgtc gatgaaatcc tcttgtgcat
     9541 aaattcattt cacgcataag aacagctaca acacgtttat tgatttcgat tccttcatcg
     9601 taaagttttt gagtgatccc tggagatccg tatcgatctt tgtgttcgta gaacactcgt
     9661 cttcctcttt tagtcgttta ttttcctgtt caagaatgta gaaatctgct ttatcaggta
     9721 atctttttcc ttgtccagca aaagcatttt tcccgtatcg acgatattct gaaacccatc
     9781 gggaaattgt tggacgagag atattgtatt cttcagcaat tttcttggta gttacttttt
     9841 tctcaaggat atcctgtgaa atctttagtt taaatttacg atcatatgtt tttctcaata
     9901 gagttcattc cttccggtaa cgtaactgat agtcaagaaa agtgtatcat agcccaactt
     9961 agtatcaggg tttgtcaact aaactgtgga agttgataga taagagtttt taacaaccaa
    10021 aattctcttg actatttttg aaatcagaca aattttgatt ggacacatag ttgaataaat
    10081 ctaccgtcgt tacggaaggt aaatcgcatg cttttcaatt ctagaggaga agtcctcgtt
    10141 gaagtaccat agcccagttc tggattcgac ctcctgccca cttcatacgt aaataaagta
    10201 attttaggag tgagactgtg acaaaagtta tttttaagct aaaaacgaat taaaaaacgt
    10261 tgataattaa aatcattcgt tttagattct atgaatcatt atagaacgct gtaaaatcga
    10321 tactttataa ttataaagaa gatagtttca aatttattgt tcgaaatttg aaactatctt
    10381 tttacttatg tcacagactc ccttagttga gttattcttt aatttcgaat ttcgagatag
    10441 ctagatatat tgcaagcata cattcttgat aacaatcttg atcatatttt ccagttctcc
    10501 aggattgctt atgaatcaaa gagctaaatc ttttgagaat tgcctcaaca gcttcttcat
    10561 ttccagcctt tgctaaaatg aaatcttctt ttaaagaatt cataaatcta ccttcttccc
    10621 atggacttat aatctatttc tttgggaagg aggagaacga gcaaagaagc atacaaaatt
    10681 agttattatg cagatattaa accagcattt tttgaatttt tttgtaaagt cgatgtttta
    10741 aatttgtcgc accctgtctt gtaatcccca gaacctttcc aatttcttta tcagttttat
    10801 caaagataaa tttttcgata agaaaaattt gttcaggttc tttcaacaaa ggaaggattt
    10861 ttgctaattt tatatcttca acaaggagag tcacatttaa aacattaatc ggttgtttaa
    10921 attttatagt atctttagta aacaaatatg gtgggagttg atcaagttgt tcatgttttt
    10981 gaatttcttc ttgtttttta taataagtat tagcggtgtt caaaatgacc ttttcaaaga
    11041 atcgaatcaa ctgggcattt acgcgttcgt cgttatctga catattgcac acctcctcca
    11101 ttaagattta aagggaaaag aggcaaaaaa cacgaaaaga agctaatgca attatgctgc
    11161 gctgcgaata acttctataa gctcacatgc attgatagga ctgctgatgt ttattttcaa
    11221 aaatgttttt ttagttgata atggatagtt atctttatac ttagaaaaga aaattcatat
    11281 atcttcttaa atatcaaa
//
LOCUS       DAZAID010000089        11283 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000089 DAZAID010000000
VERSION     DAZAID010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.091"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..1650)
                     /locus_tag="ACG9BE_002276"
                     /pseudo
     CDS             complement(15..1650)
                     /locus_tag="ACG9BE_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2089..2958)
                     /gene="scrK"
                     /locus_tag="ACG9BE_002277"
     CDS             complement(2089..2958)
                     /gene="scrK"
                     /locus_tag="ACG9BE_002277"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN3033004.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2978..4099)
                     /locus_tag="ACG9BE_002278"
     CDS             complement(2978..4099)
                     /locus_tag="ACG9BE_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFN3033005.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(4101..4496)
                     /locus_tag="ACG9BE_002279"
     CDS             complement(4101..4496)
                     /locus_tag="ACG9BE_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3033006.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(4515..5312)
                     /locus_tag="ACG9BE_002280"
     CDS             complement(4515..5312)
                     /locus_tag="ACG9BE_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3033007.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(5314..6102)
                     /locus_tag="ACG9BE_002281"
     CDS             complement(5314..6102)
                     /locus_tag="ACG9BE_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN3033008.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(6116..6592)
                     /locus_tag="ACG9BE_002282"
     CDS             complement(6116..6592)
                     /locus_tag="ACG9BE_002282"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3033009.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(6664..9321)
                     /locus_tag="ACG9BE_002283"
     CDS             complement(6664..9321)
                     /locus_tag="ACG9BE_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN3033010.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNIRKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(9385..9744)
                     /locus_tag="ACG9BE_002284"
                     /pseudo
     CDS             complement(9385..9744)
                     /locus_tag="ACG9BE_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(9922..10242)
                     /locus_tag="ACG9BE_002285"
                     /pseudo
     CDS             complement(9922..10242)
                     /locus_tag="ACG9BE_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(10358..10879)
                     /locus_tag="ACG9BE_002286"
     CDS             complement(10358..10879)
                     /locus_tag="ACG9BE_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033011.1"
                     /translation="MDKPFLDSVREYLSNPKSTLFPHEDYKIIMNYYLPIGAPQNSKI
                     KIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGL
                     DLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLM
                     ELKKYSNTQTKFS"
BASE COUNT         3602 a         2228 c         1630 g         3823 t
ORIGIN      
        1 tgaaaaaaat gtaatcagat cattattttt atatgttgta acttcctgat aagaaaacta
       61 attaaaagaa agaggtaact acataaacta attaaaaaag ctaacttcat agttggagga
      121 taaatggaag aaacttcaat gattgtttct ccttgtgttc cttcaatcga cactagtcct
      181 attatcaatc tctctttttt gtccatttac ttttgcgact gttccccaat aatcaaatac
      241 aggaagcgtt attttttgtc ctttagattc atgtactgta tagactattt tcgaatccat
      301 cacttgataa ggcatagtat gccattcctt gccaattctt acttcatgat tctgcaaatg
      361 ttctttgtat tgtaatgctt ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt
      421 cgtgtaacga ataagatttt cttgattcat taccaagtga tctctctgag tgacaacttt
      481 atgttcatag tttgcttttg ttgcagcaga tacagccaac agtaataaaa caattgccgt
      541 tatattaact acttttcttt gcttagctga tgtgctcttc atgaaaacga acgataaaat
      601 caacgagcca aacagtactt gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat
      661 gggtgtattt tgtaataaag accatggaaa aaatttggtg gataagagcc aagagactac
      721 cgcactaagg taaatcactt tttctttgcg attaaatcct ttaaaaaaga agagtggaca
      781 gatcaaagaa ataataccaa tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc
      841 accaatcaaa ctatccgaga catttaatgc ttggtgttgt aaggtcgttt tatctggctc
      901 attaatcgat tgaaccatga tttgtcggaa catcggatac caaaagtatg ctgttaaaag
      961 aatcgtcatt cctactgcct ttgacaactc cacaatcaac cggatgcttt ctttttttct
     1021 tgtttcactt actacaaagc cgcctaagac tagtaagcca acggctatta cactcataaa
     1081 agtggaaaga acatgtgtat aaatgagtaa gctcatactg actgctaatg gaattgcaca
     1141 tggctttttc tcataaatca gtgcgtaagc ataatacata actatcggta taaccgtaag
     1201 tgcaatcgct tcagcaatag cagaacgatg ataaatatca atcgtacggt acccataaaa
     1261 tgtatataaa cagctaaata taaaagccgc atcgattctt tttaaaaatt ttagtccata
     1321 atgaaacatc agcatgatag tacaaacatt caatagccaa acatataaga tataactaac
     1381 gattaaatta ttacttatcc aataaaaaat gactgccgga aaaaaggtca agtagggata
     1441 aaaataattc actccgctcc cataatgatt aaacgtcgta aaattgattg gaccagaaaa
     1501 aattgaagat aaccctttga ctctagcaaa atggaaggca atatcccccg atgtagaaat
     1561 agtatgaaca tttctaaaat aattgattga ataaagtcca gcaataattg ctaggagaat
     1621 ggacaatatt atttttttct ttacttgcat tacttaatta tatactcctt tcatggattg
     1681 ctttatctag tataacttaa attttaaata aatcgttatt tatttttact ctcacttaaa
     1741 aaagcactaa aattataaga gagggaggta atggtctgaa caccaagaat tgttccaaca
     1801 tcgaattgct taactaatga gttcaaagct taattatgtg gctcttatat ttaccgagcg
     1861 tggattgcga tctctttagc gaaacaaaca tctttcataa aaaaaacagc catatccgct
     1921 ttcttggata tgactattta ctactacttt actatctttc attcttcttg tctcacttat
     1981 gataaggcgc tgggtatcat gagtgaatga tattgctgaa tacaaatagt ccgaccacat
     2041 taagttgatc ggacttcata agcattttat tgactaatac ttttttgctt aagaattgat
     2101 tctaacatct tttgctaaag ttaagcatcc tatagttcct gcattatctg ctaacttagg
     2161 tgtgacaatg tattcttcga tatttggcgt atcgacatag ccgttaatta gttctaaaaa
     2221 acgtctttgt actttctttt tcagatgttc ttgttgcatg acaccaccac caaaaattat
     2281 aatatctggt gaaagcatca gggtcgtatt acaggcacat tgtgcaatat aatccgcctc
     2341 gatttcccag aacggatctt ctatagaaag atcttgtccc ttggttcccg tacgcatctc
     2401 gatagcgggt ccagatgcca acccttcgag acaatcatga tgaaaaggac aagtaccaga
     2461 gaaagtatct cctgaatgtc tcttaactaa cgtatgcccc atttctggat ggctaaaccc
     2521 ttcaacaaaa atcccatctt gtatagctcc tccaccaatt ccagttccaa cagtgtaata
     2581 aacaacactg gataaacctt ttccatgacc tgaaacatac tccccgtaac aagcagcatt
     2641 cacatcggtt gtccaggcaa ttggaatttc gaaatgctta ttcaacgttc caacaaaatc
     2701 aaaatttttc caggcagttt ttggagtaga ggtgatatgt ccataggtct ttgattcacg
     2761 atggatatca atcggtccaa atgaccctac tccaatgctt tctaattgtt ctttatagtg
     2821 attaaagaaa tcaattacca aactcattgt ttcattcggt gtagttgtag gaaaactcac
     2881 tctttctaaa acctttaaat catcattgcc aatggcacaa acaaatttag tcccacctgc
     2941 ttctattgag ccatacattt cttactccac cgtcctttta tctaggaatt cgttcttccc
     3001 tgtctaatag tttttcgaaa aactcatgtg gttccttttg gtaccaatag gcaacagaag
     3061 aaatatcgtc ttgacgctca taaagcttga tgtcatcatt tcctagggtt tggaaagtaa
     3121 cacgtaaatt ttctttgaaa ctaatggggt cgggtaaatg ccagcggtac aacccatgca
     3181 tcggtaaagc atcatcgcca aaaccatgaa ctggatttgg attaggtttt ccagcactaa
     3241 aataatcccg ggtgtgatct cttttagatt gaaaaggata gcccataaat agcgtattaa
     3301 aattttgagc atatgccctt tctccaaagt tctttttatg gaaagcccaa gcgccgccaa
     3361 aataatcttc tgctcctgta gatgttattg tagggaaatc ttcatctcca tctatataaa
     3421 acttaaattc tccttccccc caccagtatc tttccaatgc gcacaaagca aagtaggttc
     3481 ctacataata cccagtgcct ttgattccat caataagggt gtaatctttt ccttttgtcg
     3541 tgatgggttc acggttccat ttagcatgaa aatacatcgc atcatccggt acttcagtca
     3601 atgcataatt aaatgtataa aaaatgtgct taataaactc aggatgttca ttcgttaaag
     3661 taatcttagc tttttttcga aatggcatct caaaatagca attcatccct gatgttggat
     3721 tcactacgat tggtaaggaa ttcacttcgc atcttgtccc aaacccatta caaaaaaaat
     3781 ctcctaatgg actttctaca gaaggagtgg attcatcatc ccaataaata cgcaaaatta
     3841 aatcacgtaa gacgaaactc ccagcgattg ttttgtctgt cactgtcatc caaaaatgac
     3901 gtaaaattcc agggccttcc atttctgcta aagtaattgt ctctccagca tttatcggaa
     3961 cagaaggtga cccttttctc ccaattccta gatgactcgc tgtcattgcc gctttccctt
     4021 tttcaccatt tttattttct ggtgaaatag ttctactttc ctctcccttg tggagccata
     4081 tattatttaa acaattcatt ttattcgtcc tctatccttt cttcactttt ttgattattc
     4141 atatagatga tttgttgttg tgcctccgca ataattgctc caatatctaa attatcattt
     4201 cctaactgca aagcgattga cattgccaag ggaagattca ttcctgcaat tacgtgcact
     4261 ctgtcagaaa tataggtaaa aaacttttga tttacacttc ctccagccat gtcagttaaa
     4321 acaattattt cgtctgtagt atccactaac tctttaattt gttcatctat atcatcaatt
     4381 tcttcgacat atgcacataa gctactaatt tgattatgca tacctgttaa aaactctagt
     4441 gtctctttaa aaccctcagc tagtattgaa tggctagcaa tgatcaatct tctcataatt
     4501 caaatccttt tcctttacgc taatattcca aatgcagcca taaccatcgc aaaaacaata
     4561 acgagtagaa tcaaaatggt catactgatt tttttcttac tgagtagata atatgctgca
     4621 gcagtaataa ttactggaat taatgttggt aaaattttgt ccaacattgg ttggattttc
     4681 atactaactt caccaaaagt gaatttaagt ggcgttgaaa cactgataac tgaagcaatt
     4741 aatgatccaa ctactgttag ccccaatact gaagcagctt cagttaaata attaatcttt
     4801 tcaccgtatt cagtaacaat cttcattccc tgcttataac caaattcaaa gcctctcatt
     4861 cggaaaaaga agattaccat gattaaaatg aaccataaaa agattcctac aggattattt
     4921 tctaaagcca tataagccgc aattgatccc atgatggttg ggatcatcgt catcaaaaga
     4981 gagtccccaa taccagcaat tggacccatc gtaccaactt taaagtcatt aatcgcatct
     5041 tttgcttcta atccttgctt ttcctccatt gcaacacaag cacctaaaat aatggctgcc
     5101 agccaaggct gtgtattgta atactgaaag tgattattta aagcttcgat gtattcatca
     5161 tcatctttgt ataattttct taaaataggt gacaatgcgt aagtcacaga tgcaccttgc
     5221 tgtgtttgat aattaaaggt acaaactgac atcaaccaac gatagttcgc tttcctaaga
     5281 tcctttttcg ttacttgata tttttcgctc attttattca tcctccccaa taaacccaac
     5341 atcagaagct cccgcagaag cagtgatttg tgcatctttt tcagctctat gatacacatt
     5401 aaatgctaaa gcgaatccta tgatcgccgc tccaataata ggtaccttca aaaaagcggc
     5461 taagaaaaat ccaacaagta aataactaat gtatcgacct gtaggcataa agcgtaacaa
     5521 catcgcaata ccaacagcag gcaacatttt tccggctaat gttagcccat ccataaacca
     5581 attaggtaaa acatccaaaa cgtaattaac agcagactgt cctaaagtaa cggcaataaa
     5641 tactggaatt gctgcagata aaccaaagaa aattggcccc aaccacaaaa cactattcat
     5701 tttcttaaat tctcgtttat tcgcatattc ctgtgatttg cgaacaacaa aaccatttaa
     5761 gattttgaca attacatcta gttgcacccc taacatacct acaggtagtc ctattgctaa
     5821 tccggcttct actccaagct ggctaccaaa tgccgttgca ataattgcgc acgttccata
     5881 gtccggtaca gatgagccac caaaaccggc aactcctaat gacatcaatt gaactgtacc
     5941 gccaactatc aaacctgttt ttatatcacc catcaaaatt cctgtgaata atccccaaaa
     6001 aactgcgtaa tttacaaaga tcatcggaat cccataataa tcaacatgtt taataaatgc
     6061 taatatagtc agcataataa tttgaaaaat agtaaaatgc attatcctta ctcctttatc
     6121 ctaattttga ttgaatttcc tgtgccttat ccgccggaac aaattgtgta acaatttcta
     6181 caccagaatc caacaatttt ttatacgtgc tgatattttc atctgatgca taggctctag
     6241 aagatagctg taatgcatta tctttaacaa cagtaccgcc aacaattagt cggtcaattc
     6301 taataccgtt atctactaaa tgaaggattg tttccggctc tttcacgatg acaaatactt
     6361 tttcctgatc gtatttcccc tttttaaaat tcgcaatcgc tgtttcttca gtgataattg
     6421 atacagccat tccagcaggt ctagctaaac gcatacttga tttttggatg tcgttatttg
     6481 cgacagaatc atcgatcacc atcactctgt ttgctttaga cattggtgcc cattgcgtga
     6541 caataatccc atgcaataaa cggttatcaa ctctcgacat aacaatactc ataaatactt
     6601 cctcctttat ctttcatttt cttacaagca attaccatgc caaaacaaaa agaacttgat
     6661 aaatcaagct ctttttacat aatcataaac gtatttaacc tcatgaatcg ggatgtcgac
     6721 actataataa tgttcaacga cactgaaaga ttgcttaaaa tgtgtctgaa atttttgaaa
     6781 ttcctcactc gtttcattga aataaatttc atcttccaaa gtattttgtt ttactaatcg
     6841 ttcaattaag caactaatgt gaacatataa tccgaaacac gtattattat caaatgaaat
     6901 tcctagatca ttttgtaaga tgtatatagc atctgaaacc cgttctaata atttagtcgg
     6961 atttaatatg gtcaattgct ccattaaatt cgttagacta aaattcttca gaatattatt
     7021 gttcaaaact ttcagttcat tatcattaaa gtgtctattc aattgattgg agaattttct
     7081 tatattcatt cctaaaatca tatcttcaat tgaaaagaat tctacatctt ctagatgtgg
     7141 atttagtgtt ccaacaataa aaagaatatt gttctcttta cgaagctccg ataaatagtt
     7201 ttcattcact aaagagtaat agtcacacgt ttctatgaca agatcagtat cccttggaaa
     7261 gctatcttca agtatccttt ttaatttaaa cgaagttcct aatccgcttg cacaagaaca
     7321 gacaatcata tccttcttaa tatttgatga ttcataaata taatccacat tttgattttc
     7381 ttttacttgt tcaagaatct gtttaagcgg taaatctaat aatatctgat ttgcaacatc
     7441 aatagccatt cttgttgaaa cattgtttag tagtccgaat tttttttgag cgtctagttc
     7501 caaatttaga taaattttct ccagtgaacc catatcaaca agtaaaataa tttcttcata
     7561 tccggtacac tcttgtaagt atctattaat tttgtctgca atctcactac ttgaagtcat
     7621 tagtggcata tcaatggcgt cgtatatata tctctcaacg atcctattag cagtacttgc
     7681 tatacaggtt gccgttagat ctccatgagc caaaatcagt gctaaacgtt tttgctgaaa
     7741 ttctttacta cacattaact taaataaaat aaccaaaaca tttttttgaa tttttgttaa
     7801 ataaatatcc cctatttgaa taaaacgatt gactaattga acttctcgac tacaccaact
     7861 attaatatca ttgatgaagg aatataattt atcgtccgat gttaattgga tttgataaat
     7921 ccaagaattc aaaaagatag acgttatttg aagataataa tagtcaaatt ctatttgaaa
     7981 atctctctct atttccttaa catactgtct aaatcgaatc tcttgatagg tgggatatgt
     8041 taaagctgaa ttattttgga tatcaacatt cttttgtcta aaatatctat catatttctc
     8101 aaaaagctgc tgcttatcag tgtctttctt ccacgtttga taaagatact ctataagttc
     8161 gttgatatta aaaacttcgt ctactttttc caaatcatcc atttgaatat attttaattc
     8221 attctgtctt ttcataacct tcatgccgtt gaaattcacc cattgtggta aatgatacat
     8281 ctgaatataa agttcttcca aatcatctaa gaaataaaag gcattggcac aggtcaattg
     8341 aatgatattt ttcaaattgc caacattttc actaaagcga tagtttaata aaaaatctaa
     8401 taattgttga gatatcagta ttttctttgc tagtctttct tcttctgatt taaaaaatat
     8461 cttgattaac tgttttcttt cataaagaga acgactgtct aatgttggaa gtgttagctt
     8521 tacaggtatt cttctcaaca aagttgataa tagtttatcc tcaggtggtt ctgatgttgc
     8581 aaatataatc cagcaacttg atgtgtacca gttttcatta tcacccatcc gatgaaattt
     8641 cctcgtatcc ataaactgaa aaagtttctc ctgacattca ttgctcaatg catgaatttc
     8701 atctaaaaat aagactccat tattagctaa ttctgctaaa ccctttcttc catcttcggc
     8761 tcctgtatat gcacctttta catgcccaaa aaaattggac aagaaaagtt ctggattatt
     8821 ggcatattca ctacaattaa ctgaaatgaa agatgccttc tcttctatat aattattggc
     8881 caacaaatcc ttatacacat aatttgctaa atagcttttt ccagttccag tctcgccatg
     8941 aagtaaaatg ggtaagccat ttggcggata gtagattgct gctttaattt gagcaattac
     9001 atttcgcaga cttccttgac ttccgataac cctttctatt ccaaccctaa aagagctatc
     9061 ataccatttt tccgcttcct ctgaagagct aaaaatttgt tgatttggaa ctttctccgg
     9121 taaagaaaag aagattactg gacgagtatt gatttttcca aattctccat cttttacgaa
     9181 ttcattcaaa tattgagaga caatattacg actaacattt aatttttcag atattttctg
     9241 tgctgtattg ttcccccatt tatttttaat ctcagagttc tctatgacat aattgacaat
     9301 gcgttctttt aattcttcca tcacagcacc tcctcgacac tattcgaatt ttgccgacag
     9361 tatttttcca taaatatgac ttgctaaaaa ggctttgttt agatcattca attggaacgt
     9421 tgcttgtttt gcttgatccg ctaagacgtt gaaaagatac tctggcgaaa tcgcataccc
     9481 caaaatatca acgattgttg caatcagatc ttcttttgat tcttcatctg aaagtaattc
     9541 acgatacaac atcgtttgtt tgcttactgt gtcatattcc tctaaggatt catctgctaa
     9601 aagaaccact tgttccaaca aacggtcgga taaatattta tagaaaatca gccccaataa
     9661 ataattttta tattctgacg catccatttt actacggaga ttatctgccg cactaaataa
     9721 cttctgattt aattctgcac ccaaaaagat acactcctat ctcttattta aagttgctat
     9781 ataccctctt attttagcat actcagtttt agaaaaagca aaaaaataaa gaaacttatt
     9841 cttgataaca agctacgtga cgtatatccc taacacaaaa atagccatat cctttggttt
     9901 ggatacggct atttgatatt actcattcaa ttatcaaatc acagtaggtg tgaatctcat
     9961 cgggatcggc aatatctact tctgattagg atcaaaggtg attttctcaa taaaggtgtg
    10021 atacaaagct ttaacttgct tcttctcact ctggataaga aagcaatcca aactagtcaa
    10081 tatctgttgg acatctttga cttgaatcgg ttccccttgt tgttgaaaga tccctaagat
    10141 ttcattctct cgaatttgta actgtcttcg tttttcagtc aattcattta aacgatcttt
    10201 taacatcgga aacaattcgg gactatcttc caaagcagct tcctgttttt gctctaatag
    10261 ttagattttg cgtaccattt tcttctttta ttttccttcg attgctgatt ttaatatagt
    10321 ctaaaggaaa tttatattta ttgtattctt tataacttca agagaacttc gtttgagtat
    10381 tactgtattt ttttaattcc ataagaaaat tagttattac ctgccaccta atcagcaaat
    10441 ctgaacctga gtcaatataa ttaatattat tttccatctc caacattgta agtactgttg
    10501 gtgttaatat agggactttg tacaattgta tttttttgat tttatcatct gcacaaagga
    10561 gattttttaa atctaatcct tcttttattt caaaagttga attttttaaa tctataagag
    10621 taaaaccaat tccttcatac tgaatttctg aaaatcgaat ttttctccca gttgatagat
    10681 caaggggcag aaaacctttt attacattgt gagtgtcacc atcatttaca atgtcattag
    10741 gaattttgat tttagaattc tgtggagcac ctattggaag ataataattc attataattt
    10801 tgtagtcttc atgaggaaat aaagtacttt taggattgct aagatattct cgtaccgaat
    10861 ctaagaatgg cttatccatt gaatcttcat ccccatttaa actaaaaaac attcctacaa
    10921 ttgcttttgc aactctaagt gaattcattt tctttattga atgtgttaaa tcagacttac
    10981 cattctttaa acttgtattg ttataaagga aatttttatt gtcttctgca aatctaatat
    11041 attcctcaac atactcacta ccaaaaaaac tattacattt actacataac gtattggaat
    11101 ggcaacagtt tttttgacaa attttataag gtgcagaact tctttccgta tgctattccg
    11161 attgtccttg acaatgagcc tcctcggatg tgacgatctt cggcaggagc taacagttaa
    11221 agttagactg cctcgctagc gttagcacat ccgagctcat tatcaaggat tcgctgcgcc
    11281 aat
//
LOCUS       DAZAID010000090        11130 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000090 DAZAID010000000
VERSION     DAZAID010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.092"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..1351
                     /gene="lysA"
                     /locus_tag="ACG9BE_002287"
     CDS             92..1351
                     /gene="lysA"
                     /locus_tag="ACG9BE_002287"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFN3033012.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1338..2138
                     /gene="dapF"
                     /locus_tag="ACG9BE_002288"
     CDS             1338..2138
                     /gene="dapF"
                     /locus_tag="ACG9BE_002288"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFN3033013.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2141..3022
                     /gene="dapA"
                     /locus_tag="ACG9BE_002289"
     CDS             2141..3022
                     /gene="dapA"
                     /locus_tag="ACG9BE_002289"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFN3033014.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3019..3735
                     /gene="dapB"
                     /locus_tag="ACG9BE_002290"
     CDS             3019..3735
                     /gene="dapB"
                     /locus_tag="ACG9BE_002290"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFN3033015.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3741..4436
                     /gene="dapD"
                     /locus_tag="ACG9BE_002291"
     CDS             3741..4436
                     /gene="dapD"
                     /locus_tag="ACG9BE_002291"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFN3033016.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4452..5453
                     /locus_tag="ACG9BE_002292"
     CDS             4452..5453
                     /locus_tag="ACG9BE_002292"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFN3033017.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5450..6811
                     /locus_tag="ACG9BE_002293"
     CDS             5450..6811
                     /locus_tag="ACG9BE_002293"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFN3033018.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6771..7922
                     /locus_tag="ACG9BE_002294"
     CDS             6771..7922
                     /locus_tag="ACG9BE_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN3033019.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7897..9066
                     /locus_tag="ACG9BE_002295"
     CDS             7897..9066
                     /locus_tag="ACG9BE_002295"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN3033020.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9241..10620
                     /locus_tag="ACG9BE_002296"
     CDS             9241..10620
                     /locus_tag="ACG9BE_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN3033021.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10901..11130
                     /locus_tag="ACG9BE_002297"
                     /pseudo
     CDS             10901..11130
                     /locus_tag="ACG9BE_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3703 a         1945 c         2494 g         2988 t
ORIGIN      
        1 attttgttta atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa
       61 acggaaaaaa agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac
      121 agcaagcaca ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat
      181 gagacaaacg atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt
      241 gctttacgcc tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg
      301 attcgggcta gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc
      361 tagagatact atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact
      421 tgaaaatgat ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga
      481 attaagccaa cgctatttaa aaaaaatctg tatcatgctt cggttgaatg tcggagtaga
      541 agcacatact catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta
      601 tgaaagtaaa gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt
      661 agaaggattt catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga
      721 aatcgacaaa ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg
      781 cggagggttt gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact
      841 aaaaaaactg attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca
      901 attactgatc gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat
      961 tggtttcatc aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga
     1021 taatatccgt ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc
     1081 ggaagaaaaa acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt
     1141 aattaaagaa gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac
     1201 gggagcttat ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt
     1261 ttttgtcaat aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact
     1321 aaggggggaa gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct
     1381 tattagatta cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag
     1441 aaaaatatga tatagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt
     1501 acaacaaaga tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat
     1561 acgtaaatga tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca
     1621 caagaaaaat tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc
     1681 cagatgattc tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa
     1741 tagagatcaa tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcataccg
     1801 ttgtttttgt tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc
     1861 atcatccttt gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag
     1921 aattgatcgt ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt
     1981 gctgtgcctc ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg
     2041 ttcacttgga acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc
     2101 cagcagttca tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt
     2161 ggcgctgatc acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt
     2221 gatccaattc catttagatg aacaaacaga tggattatta gttttaggta caacaggaga
     2281 atcttctacg ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt
     2341 ggctggccgt gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga
     2401 aaaagcgaag cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta
     2461 caacaaaaca agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc
     2521 gattccgctt attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt
     2581 attagtaaat ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat
     2641 ggcttatgtg atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa
     2701 tgatgatttg atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc
     2761 gaatatccaa cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca
     2821 gcaagcaaaa gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac
     2881 caatcctatt cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg
     2941 cctgccttta gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa
     3001 agaaaggacg gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga
     3061 gtcgaagaag ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa
     3121 gaagcaacta cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca
     3181 gataatttag agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc
     3241 acgacaggtt atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct
     3301 gtgatgtata gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa
     3361 gcagctgcaa tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa
     3421 aaagatgcac catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag
     3481 ctgcagcccg tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac
     3541 agtattcgtg caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa
     3601 atatttacga tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa
     3661 gcagcggact ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg
     3721 gacaaagggg tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat
     3781 ctaaaaagac gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa
     3841 gtaaagtttt tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc
     3901 cggttttaga agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat
     3961 acagcgcagt tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta
     4021 ttatcagaga tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca
     4081 atattggtgc agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac
     4141 gagcgacagt gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg
     4201 aaccagcatc ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag
     4261 tgattgttga aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt
     4321 tagaagatgt ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa
     4381 gtgatgatca gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga
     4441 gatggagaaa aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc
     4501 aaacgatgct caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag
     4561 cttcaaaacg ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg
     4621 aattgacaga aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg
     4681 tatccaaaga gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct
     4741 cagcatggcg ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact
     4801 atacattgaa tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc
     4861 ttaaagcttt acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag
     4921 tttctggttc cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac
     4981 cagttttgta tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg
     5041 aaaatgggta tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc
     5101 agcctgcttt gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag
     5161 tctctgtggc agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca
     5221 gccagtttcc agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca
     5281 ttcttgctag aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag
     5341 agaacggatt gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg
     5401 ccgtacagat tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac
     5461 gctgaaagta gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt
     5521 ccgtacaatt gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa
     5581 acgtacgtca gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca
     5641 gcatcatgtt gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag
     5701 agatgttctt gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact
     5761 ggaagacgaa aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca
     5821 actgatggct gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga
     5881 ctatgatggg aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag
     5941 atatccgaaa atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt
     6001 attgggccgt gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga
     6061 aagttatgag aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga
     6121 tcatcctaaa atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg
     6181 ggctaacgtt cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca
     6241 aataaaaaat accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga
     6301 aaaggaaaat ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt
     6361 caaacgtcat atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa
     6421 acatgagata tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc
     6481 agcagaagcg attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg
     6541 cgtggaagaa atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca
     6601 taaagaattg attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg
     6661 cacatcgatc ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa
     6721 tcaatatgaa acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag
     6781 aatatcgaat ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa
     6841 aatatttata tgaacaagta aaaaacagcg aggccgttat ccatgaaatc ggcgaaacag
     6901 gactactcct ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg
     6961 atgcactgcc agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata
     7021 tgcatgcatg tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa
     7081 ctaaacacat tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa
     7141 tttccggtgg cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag
     7201 caatatttgg ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag
     7261 gacctttgat ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata
     7321 ttgcttctag tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg
     7381 tttatgattt tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa
     7441 tccaaggtgg aacgatccga aatgttttag ctggagaagt tattgtatcc ggaagtatcc
     7501 gttcttacag ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac
     7561 agtttcactc tacagctact atttcttttc gctataatga cggctatcca gctgtaatca
     7621 accatagagg cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc
     7681 cagttcttca agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct
     7741 ttttaggtgt cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg
     7801 ttttagaaaa tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa
     7861 aaatcgatag cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt
     7921 agtcctaggc tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg
     7981 tcgataccag atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat
     8041 gtcaagcaag caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga
     8101 ggattagagg aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact
     8161 tatgatccgc aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg
     8221 ttgttatctg ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga
     8281 tatcagccat tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca
     8341 gggtttgtct gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc
     8401 aaagcagtca ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa
     8461 ttgaaagcaa tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta
     8521 tatgctgaat tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa
     8581 acaatcgtga taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt
     8641 atttttgctc aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact
     8701 gccgctacaa cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat
     8761 agcgagaata tgaaagagca atatcaaaca cgaagagact atctggtcca gcaattggcg
     8821 cctttaggat ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca
     8881 gctgccattc aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt
     8941 gcgtgtatcc caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat
     9001 gctagcagca tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa
     9061 cagtgattca tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg
     9121 agacgattgt tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg
     9181 taatgttaag tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca
     9241 atggaaaaaa tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg
     9301 gaactattga cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat
     9361 atttccttca tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat
     9421 gatgtacgta atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat
     9481 cacgaagtaa aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat
     9541 aacgaaacaa aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac
     9601 aatctgccag taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg
     9661 gcaaccaaaa tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt
     9721 gcgggttaca ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta
     9781 ttgcttgatg tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt
     9841 ttagaacaac acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct
     9901 tttactggta acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat
     9961 ctagtcattc aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac
    10021 ttagatgaac gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg
    10081 tatgcagtag gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc
    10141 gctttagcga ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa
    10201 gtaccaaaca aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat
    10261 cattttgcag caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga
    10321 gtgtcttatt atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca
    10381 aaagtgtcca tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta
    10441 ttatctacct atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa
    10501 ctgactttag aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa
    10561 tggagcatgt tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa
    10621 gaaaacgaaa tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag taataagaac
    10681 agccaagtaa aaacagcttc ttatgttttt aacttggctg gacttattac cgcagatgca
    10741 agcttttgaa caccgtttat tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc
    10801 ccaaaagtta gaccaaaaaa tctaactttt ggaggtcggt attttttatg gttaaatata
    10861 cttttgaact taaattaaag atcgttcatg attatttgga tggcaaaggc gattggaccg
    10921 taaaaatcca gatcaagaga ttgaagcgga aatgcttaaa attcgggaga agcacaagga
    10981 ttatggctgt ttacggatga ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa
    11041 gaaagttcag cgcttgatca agaagctagg aataaaagta acggcataca cgcgtaaatc
    11101 acgtcgctac aattcttatc gaggaaaagt
//
LOCUS       DAZAID010000091        11120 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000091 DAZAID010000000
VERSION     DAZAID010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.093"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            113..568
                     /locus_tag="ACG9BE_002298"
     CDS             113..568
                     /locus_tag="ACG9BE_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033022.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            930..1205
                     /locus_tag="ACG9BE_002299"
     CDS             930..1205
                     /locus_tag="ACG9BE_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033023.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            1218..3296
                     /locus_tag="ACG9BE_002300"
     CDS             1218..3296
                     /locus_tag="ACG9BE_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN3033024.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            3459..5342
                     /locus_tag="ACG9BE_002301"
     CDS             3459..5342
                     /locus_tag="ACG9BE_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN3033025.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            5354..6301
                     /locus_tag="ACG9BE_002302"
     CDS             5354..6301
                     /locus_tag="ACG9BE_002302"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFN3033026.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKRPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            6321..6848
                     /locus_tag="ACG9BE_002303"
     CDS             6321..6848
                     /locus_tag="ACG9BE_002303"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFN3033027.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6911..7564)
                     /locus_tag="ACG9BE_002304"
     CDS             complement(6911..7564)
                     /locus_tag="ACG9BE_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFN3033028.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7696..8538
                     /locus_tag="ACG9BE_002305"
     CDS             7696..8538
                     /locus_tag="ACG9BE_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN3033029.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8696..9592
                     /locus_tag="ACG9BE_002306"
     CDS             8696..9592
                     /locus_tag="ACG9BE_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN3033030.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            9595..10308
                     /locus_tag="ACG9BE_002307"
     CDS             9595..10308
                     /locus_tag="ACG9BE_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFN3033031.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            10328..10846
                     /gene="msrA"
                     /locus_tag="ACG9BE_002308"
     CDS             10328..10846
                     /gene="msrA"
                     /locus_tag="ACG9BE_002308"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN3033032.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10843..11070
                     /locus_tag="ACG9BE_002309"
     CDS             10843..11070
                     /locus_tag="ACG9BE_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFN3033033.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
BASE COUNT         3868 a         1893 c         2302 g         3057 t
ORIGIN      
        1 aagtgggcag atacgtcatt agaagaaaaa acataagatg tgaccactta cataaatttg
       61 tgctaaactg aggtagtaca agaataaatt acatcataaa ggagtctttt taatgagtaa
      121 agaaatgaca gcgctaaaat tttattttag aaatggagaa acctggacga tcaaccgccg
      181 tcatatcggc gatctttgga tcaaacaaat cacaacaagt tttggacgaa tcaatggcag
      241 tgaatttgtt gaaatccatc cgtgtgctgg atttaaaatc gaaatcttcc atgaagggga
      301 cgcagttgcg acacatgata tcaacctagg cggactggaa atgggcatgt ttaatcgcgc
      361 attgaaatac gaagatatcg agcgaatgga aatcctttac cgaaatggca cacctgatct
      421 tgtgtacttc ccatatctag ataaaggaac agaaggatta gacaatcaat atcaatcaac
      481 aaaaatcagc gaaaaaacag gtaacttgta tatcgtgatc aatccagatc aacgtgtaga
      541 agatgtatac ggggaatttt tcgaataaat cgatttttac gcaaaaatga ggctgtgaaa
      601 gagatgtgtc ttattactac tgataggctg tctcttccac accgtttatt cagaataagg
      661 gactgtgaca agatttttgt cgcagcccca tttttttgtt gtgataaaag aacaagaaag
      721 gcaggaatgg gaaagtaaac atccttttgt cagaaacttt tatctttctt aagttttact
      781 tgatatctga tactatttgg aatagaatga ggcgttgaaa aatttatgga aatttctaac
      841 tagcaataaa cgatgaaaca atgaaatatt tttcatccaa aaagaacttt gctataatga
      901 tggaagtttg tgtataggag atctggaaaa tgagagagag ttttgaacaa caaaaaaaac
      961 tgttgcatga tcgctatggc gccctttcga tggatgaccg ccgtcaaata ctttgtaagt
     1021 tgcgtaaaag aaatatattg atgtatcgtc agttggaacg tctgaaacat gatctgcttc
     1081 gtttggagtc aaaacgtgta cagtgtgaat tagaggggaa ccaaacacag gttgaagtag
     1141 tcgaaaccaa aattttaaag aaaaaagaac aatttttaaa aatgcttaca caaaataaaa
     1201 aataaagggg atggacgatg gaatttgctg agttagttat tgtatttgca gtaacgatca
     1261 ccttatcgaa catagctagc cgcattttgc caatgattcc agctccactg atccaaattt
     1321 tccttggagt ggtattaggt ctgacagagt ggggacaatc aatcgatttt gaacctgaat
     1381 tgttcttagt tatgattatt gcaccattgt tatttagaga aggagaaaaa gcagatattt
     1441 cttcgattct caaaaatttc gggacaattt tgttcttggc atttggagga gtcattttga
     1501 cccttgtcgg ggtaggtgcg acgttatcat tcctgttgcc aagtgtccca cttgctgcct
     1561 gctttgcttt tggagcagcg ttaggtccga cagatgcggt agcagtcagt tctctttctg
     1621 gacgagtgaa tatccctaaa aaggcgatgc atattttgga aggagaaggt cttttgaatg
     1681 atgcatctgg tgttactgcc ttccagtttg ctcttggtgc gctgatcacc ggatcttttt
     1741 cagcagtaaa cgcggggatg agtctagtag tttctagtat tgggggagct ttgatcggct
     1801 ttttgcttgt ctggttcaaa caaaaaataa ttcatttgat cgaaaaagca tcagcacaag
     1861 atgtcacagg gtatctgctg atcgaacttt tgcttccttt tctagcctat gtattggcag
     1921 aggcatttga tgtatctgga atcattgctg cagtggcagc aggtattctc caagcttctg
     1981 gttttaggaa aatctctgtg tttgatgcag aattgtcgag cttgtcccac agtacatgga
     2041 caactattgc ttttacactg aatgcattag tatttatatt tttaggaatc gaattgacgc
     2101 aagtgttctc gccagtatgg ggagacggtt tatatccaaa cggtctttta ttagcgatta
     2161 tcgtattaat ctctgtcatg ctttttgtga tcagattcat ttctatcagt cttttttatg
     2221 tattcaaaga tggcagtaaa aagttcaaaa aacagttgaa cgagatcttg atcttaacat
     2281 ttggcggtgt aaagggaaca gtcagtttgg ctacgatttt tattttgcca ttttcaatca
     2341 ataatatgat gttttaccag cggtctttgc ttttgtttct gacggcgggt gtcatcttag
     2401 tgactttggt catagggatc attgtcttgc ctatgctgac ggaaacagag gaagcgaaaa
     2461 gcacggattt gaatgctttg atgattttag aagaggtcgt tgaagttttg cgaaaagaaa
     2521 taaaggaaat tgatcgtaat acaaaagaat ttttagccac tgaagcagtg atagaaaatt
     2581 atcaagaacg gatacgtgat ctgtacttgg aagatttaac cgatgatgaa aagcaagaag
     2641 tacaagaaat acaagcactg attttgtcga tcgaacggga tggattggat gaaagttatc
     2701 gttcgggcaa gctttcttct aatggttatc gtttttactc ccgttttctt tctcgattcg
     2761 aacattctat cacaagccag attttgtctt ttatcggctt ttggttcatt gttgtccgta
     2821 ggttaatgcg aatcgttctc catccgaaaa tgttttggca aagaagatat gctgatcgtc
     2881 agacatttat tagtgatgaa gatatccaag aaatcagaga gacatatcaa aaaaatactc
     2941 agttgattat tgaaagttta gataacttga ctgatattta tgatgaaacg atggttcaat
     3001 tttttatcca gcaacgcaaa cttgaaggaa taaaaatgaa ctcggggaat ttgatttctt
     3061 cgtggatgat ccagcaagat agtctgttta cgaaaaaaat gctaagaggt tattatcttg
     3121 aaagaaaaat cattgatgaa tacgaagttg ctgaaaaaat cacgacgttt tctgcgaaca
     3181 gctatcgcag gaatatcaat ttgttagaat cttatacgat gaacaaacca tctgataact
     3241 tttctttccg atttgcgttt cgagcaaagt caaaaaaaac gagtccaata aaataaaaag
     3301 gtaaaaattt tcaagtttta taaaaaacaa aacatatcag aacaggatcg aatcggtcct
     3361 gttctttttt tgagaacaca aaaagaacaa ataccaaaaa aattttcctg aagttcacta
     3421 tttttatgat acaatatcca agataaggag ggaacatgtt gaaagaatta aaagtaactg
     3481 acctcaaaaa aacttatggt gaaaaagatt tgtttgacca gatttctttt ttggtccacg
     3541 aaaaagaccg tatcgggttg attgggacga acgggacggg taaaacaagt ctcttaaata
     3601 ttattgccgg tatcgatagc agcgacggtg atcggcaaac agtgttttat ccaactgatt
     3661 accgtattgg ttatctttct caagcagctg aattttccga tgaattaact gttctccaag
     3721 cagtctttca aggaaacagc cccttgatcc aaactgttcg agcatatgag gaagcattga
     3781 tcgaactagg ggaaaacgga gaagaccctg atgtgcaaaa acgttatgct aaagcagaag
     3841 aacaaatgaa taaagaagat gcttggacta cagataccaa cgcaaagatt attttgcaaa
     3901 aattaggaat cagtgaatta gataaaaaaa tcagtacttt atcaggtggt cagaaaaaac
     3961 gagttagtct ggcgcaagtg ttgattgatg aacccgattt actattacta gacgaaccaa
     4021 caaaccattt agattatgaa gcgatcgagt ggttggaaaa ctatttgaaa caataccgtg
     4081 gcgcactatt gatggttacc cacgatcgtt attttctaga tagagtagca aaccgaatct
     4141 tcgaactttc atttggaaaa ttatacgagt ataaaggaaa ttatgaagct tacgtcttag
     4201 aaaaagcaga acgtgatcgt gtagaagtag aacaagaaga aaagcgcaag cggctttata
     4261 aacaggaatt agcttggatg agggctggcg tacaggcacg tggcaccaag cagcaagcac
     4321 ggatcaaccg tttcgaagat ctaaaggaga acttgtacca agtaaaccaa gaagatgatt
     4381 tagagctgaa cctagcgaca caacgtttag ggaaaaaagt actggaaatc aaagatggca
     4441 gttaccgtat aaatgaccaa acactgctag aacatttaga tttattgatt caatctcggg
     4501 aaagactagg tattactggt aaaaatggtg cgggaaaatc gacattgctt aatattctag
     4561 ctggcagaat tcccctggat agcggtacaa tgtctatcgg tgaaacggtt catcttgcgt
     4621 attatacaca agaaaatgaa gaaatggcac cagataaacg gatgattgct tacttacaag
     4681 aagctgctga agaagcaaaa acagcagatg gcagtcaaat cggagtagca gaattacttg
     4741 aacgattttt atttccaaga tttatgcatg ggacactgat aggcaaactt tcaggcggag
     4801 aaaaacgccg gttatttttg ttgaaactgc tgattcagca acctaatgtt ttattattgg
     4861 atgaaccgac caatgatttg gacatagcaa cattgacgat tttggaagat tattttagaa
     4921 gttttccagg tgctgtgatc actgtttccc atgaccgtta tttcttggac aaggtagcag
     4981 ataagttatt agtattccaa ggaaacggaa aacaagaact ttattacgga aacatgtcca
     5041 gttatttact gaagcaaaaa gagacccagc agcctgctga aaaagcgaaa ccaaaaatac
     5101 agagcaagga acctgctgaa aagaagaaat tatcttacat ggaacaaaaa gaatgggaaa
     5161 cgattgaaga tgagattgca gaacttgaag aaaaaatctc attgctccaa gaagagatga
     5221 atcatcaagg agataatttc actcgattac aagaattgca aaacgacgta tctgaaacag
     5281 aagcacaact tgaagaaaaa atggcacggt gggaatactt gagtgaatgg gtagaagatt
     5341 aggaggaaaa aggatggaac aagcgtatct tgacttagga agaaaattgt tggaagaagg
     5401 gcatcgtaaa gaagacagaa caggcacagg cacattaagc ttattcggat accaaatgcg
     5461 gtttgatctg caaaaagggt ttcctttgtt aacaacgaaa cgagtacctt ttggattgat
     5521 caaaagcgaa ttgctttggt ttttaaaagg agatacgaat atccgctatc tattacaaaa
     5581 taaaaaccat atttgggatg aatgggcatt tgaacgctac atcaaaaggc cagactataa
     5641 tggaccagat atgactgatt ttggccatag atgtttgaca gatcctgaat tcaatgaaga
     5701 atataaaaag cagagcaagc ttttttgcga gaagatccta accgatgatt cctttgctga
     5761 aacatacggc gatcttggac acatctacgg ctatcaatgg cgtcactggg aaacaaaaga
     5821 cggtggcttt atcgatcaaa tcaaacaagt gatcgaagca atcaaaaaga ctcctgattc
     5881 tagaagattg atcgtttctg catggaatcc ggaagatgtt cctagcatgg ctttgcctcc
     5941 ttgtcatacg atgtttcaat tttatgttca agagggacgg ctaagctgcc aattgtatca
     6001 acgcagtggg gatgtgtttc taggcgttcc gttcaacatt gcaagttatg cgttgctgac
     6061 tcacctaatt gcacatgaaa caggtttagc ggttggcgaa tttgttcaca cattaggaga
     6121 tgctcatctt tatatcaatc atatcgaaca aatgaaagaa caattgagcc gagaaatacg
     6181 cgcatttccg actttagttt tgaatcagga aaagcagtca gtatttgatt ttgagatgga
     6241 agatatccaa ttagaaggat atgacccaca tccggcgatc aaagctccta tcgctgttta
     6301 gaatcagaaa ggatgagaaa atgttagctg caatttgggc acaggatgaa aacggattga
     6361 ttggaaaaga agaccgtctt ccttggcgtt tgccaaatga tctgaaattc ttcaaacaaa
     6421 tgacagaagc aaatacacta gtgatgggac gaaaaacatt cgaagggatg ggtagccgtc
     6481 cgttgccgaa tcgtcagacg attgttttga cgagagattc ttcctataaa gcagaaggcg
     6541 tgcatgtcat gcatagtgtg gaagacgtgc ttgctctgga aaaagaaaca gacggtatct
     6601 ttttcatcgc tggagggtca gctgtttatc aagaatttct gccattttgt acgatattat
     6661 accgtacagt catccatcat gtattcgatg gagatgctta ttttccacct gttgactggt
     6721 ctgattggtc actgatcaat actagtcaag gagaaatgga tgaaaaaaat cgttatccgc
     6781 atcaatttga aacgtatcag cgtaatgaga atcctgtaaa aagcgggaca ggcgaattga
     6841 cgatataaaa aagctatgga tagactagct gttatttaga gctgtcccat ccatagcttt
     6901 atttttttta ttaagtaaag aataaaacag agaagtacat aaaacctgca cctaacataa
     6961 caaacaaatg ccaaacaaca tgcatatagc ggatagaatt caagctataa aagaatgcgc
     7021 ctatcgtgta agaaagcccg cctgctacca tcaaaataac accagtcatt ccaagagagt
     7081 gataaagagg aacagcagcc acgacacaga gccaccccat aaaaatatag atgatcgtag
     7141 atacttttga tactctctcc cgtttatgca acgtcaacgc tttatatata atgccaagaa
     7201 tcgcaaacaa ccagattaat acaaataaag tccaacctag ccatcctcgg atactcagca
     7261 aacaaaaagg agtataactt cctgcaatca aaagaaaaat cgaactatga tcgaatactt
     7321 ggaacacctt ttttgctttt gtaaaaatca atgaatgaaa caaagtagac gttaaaaata
     7381 aaagaatcat catcgatcca tagatagcat aagagacaat atggagggag gaatgcaagt
     7441 gggcaccttt tactaaaaga accactagtc ccgcgatact caatccagtc ccaattccat
     7501 gggtgactgc attaaacact tcatttaata tttcatacgt tcgattaaat ttcgtttttt
     7561 ccatgaaaac ggctccttta ctaggtgtaa ttaatgaaaa atcattattt gtctgttata
     7621 ctaaaaatag aaattgtctt acatgtagtt taacatgaat aaggcatctt gttaaagaaa
     7681 gagagagaga aaataatgac aaatgtaaaa atcgtaacag attcttcatg tacgatggaa
     7741 aaaaatgtac gcgatgcttt aggcattcgt gtcatgccgc tatccatcat gatcgacgga
     7801 acagtatatc ccgatgatga ccaactagac ggcgaaagat ttatggaaat gatggcgaaa
     7861 gcaaaaaatc tgcccaaaac aagccaacca ccaattggag aattcatctc gatgtatgat
     7921 gagctaggag aagacggcag tgaagtgatt tcgatccata tgacaaaagg cttgagtgga
     7981 acagtagaag cagcaagaca agccagtaat ctaacaaaaa cgaaagtaac ggttatcgac
     8041 agtgatacca ctgaccaagg attatctttt caggtaattc gagcagctga attagcaaaa
     8101 gaaggtaaaa cagtagagaa aatccttgaa gaagtcaaaa aagtacaaga caatacaaaa
     8161 ttatacatcg gaatatccac actagataac ttggtaaaag gtggaagaat cagccgaaca
     8221 actggtttgc tgtctagtgt tttgaacata cgcgtggtga tgaatttcgc ccattctgaa
     8281 ttaatccctg taactaaggg gcgaggaaag aaaacgttcg ataaatggtt taataatttg
     8341 aaaaatgaac tgaaagaatt gccaaatgtg cgacaaatcg gtatttcaca tgcggatgca
     8401 aaagaattgg cgcaagaatt caaagagggt ttgcaagaat tattcccgga catgcatatc
     8461 cctgtactgc atacgaatcc ggttatcgct acgcatactg gtaaagacgc gtttgcaatc
     8521 atgtattatg tagattagtc gaaaagagga taagccctca gcctgaaagg cttgcagctt
     8581 attctctttt tctttataaa attaggaaaa taattgtttt cttttttcat aaagactacc
     8641 tttttgataa aatttttgat aacattagaa ccaatcgaat acaagaggtg aaaaaatgaa
     8701 attaacgaaa cgacatttga tcctcgtttt gttcccatta ttaattgcga tcatcatcta
     8761 tgcaggttta gctgcttcta taccgaaagc tggcccacta atgaaaccag taactactgt
     8821 gagagaagat ccaaataaaa aagaaaaact tcactacata gctatcggag attcgctgac
     8881 tgaaggagta ggagatcaaa ccaagcaagg aggattcgtt ccccttgtcg caaatgatct
     8941 aaaagaccgt tataatttga ccgcggttga aacagaaaac tatggtgtaa atggtgaacg
     9001 aagtgaccag atcttaaagc gagtaaaaac aaaagaagaa attcgaaaca atatcaaaac
     9061 agctgatttt atcacactga ctgttggcgg taatgatttg atgaaagtta tccaaaataa
     9121 tttattcggg ttaacggtca attcctttaa aaagccactg aaaaactatc aggaaaatgt
     9181 cacagaacta ttggaagaat ttcgatccct caataaaacc gcacctattt acattttagg
     9241 catctacaat ccattttatt tgaactttcc tgagatcact gacatgcaga caattgttaa
     9301 caattggaac aaaggaacag aaacggtgac aaaaaaaatg ccgaactgct actttatccc
     9361 aatcaatgat ttgctttatc aaggattgaa tcaggaagta ggtgtcacag aagaaggaac
     9421 agatcaaaca actagcacga cagaaaatct aaatggcatc aaaaataatg tgctctatga
     9481 agaagatcat ttccatccaa ataatttagg ttatcaattg atggcaaatg cagtgagaga
     9541 tcagctgatc gagacacaaa accaatggtt agagaaggag gatgatcaat aaacatgaaa
     9601 acaagatctg acgtatcttc aaacaaaaca aagaaaacaa attactggaa attcgccttc
     9661 cttatccttc ttggggtagt acttggttcg accgtatttt taggcagccg tatttttgct
     9721 aatcgggaac cagaactccc agaaatacca gctcttacgg aaagacaagg agatcctgtc
     9781 ttaacgatca attcaaataa agaaaaagtc aatcaaatca tcagcttttt ccttagtgag
     9841 tatcaaaaag atagtgatat tgaatataaa ttttatttgg aaaatgaagc actactcaat
     9901 ggtactttcg aagtactagg ttttcctatc gatttctatt tatattttga tccgtatgtg
     9961 atggaaaacg gaaatgtcca actaaaagct agaagtcttt ctatcggaac attgagtttg
    10021 ccaatcaaag atgtgatgaa tatgatcaag agaaactata aattaccaga ttggatcgaa
    10081 gtcaatacag atgatctgac tatcatgctt aggttggatc aattccgaat gcaaaacggg
    10141 atgtatatta aagcagataa aatcgactta gtaaatgatg acattcggtt tagtctatat
    10201 ttgccaaaag atacctctga tgcttcaaac aactcaaata ccaacaaaac tagcaccagc
    10261 agtactgaaa gcaagacaaa agaaaataaa aaatcatcgg atgagtagta ttagaggggg
    10321 aatcataatg gaacgagcaa tatttgcagg aggctgcttc tggtgtatga tccagccttt
    10381 cgacacgctt cctggtatcc atacagtgat gtcaggatat actggagggc atgtaccaga
    10441 tccgacttat gaacaagtaa aaagccaaac gactggacat acagaagcag tggagattat
    10501 ctatgatcca acattgattt catatgaaca attagtagaa ctttactggc agcaaactga
    10561 tccaactgac gctttcggac aattcgagga tcgcggtgac aattatcgcc cggtgatctt
    10621 ttatttgaca gaagaacagc ggatcattgc tgaaaaaagt aagaaacagt tagctgaaag
    10681 tggacgtttc gaacagccaa tcgttacaaa aatcgaacca gcccaaccat tttatctagc
    10741 agaagaagac catcaagagt attataaaaa gcatcctgaa aattttaaaa gaaaccatgc
    10801 acgccgggct gcctttatcg aagaaaattg gaggaacgaa gggtgaatcg aagctttttt
    10861 cattatttaa tgacactgcg aggcggtggc acacatgatg ctttatctgt tttcgctaca
    10921 gaagccggta aagacatcca atttcctaag cacagctcta gctacgaaga aatttctaat
    10981 tatctggaac tgaatgtcga ctatttgccg tccatggata tctttgacga agcatgggaa
    11041 aaatatttag aaaataatca tgacatataa gagatagttt tgtactttat gatcaaaaaa
    11101 gttttttgtc caatagccta
//
LOCUS       DAZAID010000092        11042 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000092 DAZAID010000000
VERSION     DAZAID010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.094"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..1587
                     /locus_tag="ACG9BE_002310"
     CDS             28..1587
                     /locus_tag="ACG9BE_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFN3033034.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(1698..3629)
                     /locus_tag="ACG9BE_002311"
     CDS             complement(1698..3629)
                     /locus_tag="ACG9BE_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFN3033035.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3647..4561)
                     /gene="pfkB"
                     /locus_tag="ACG9BE_002312"
     CDS             complement(3647..4561)
                     /gene="pfkB"
                     /locus_tag="ACG9BE_002312"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFN3033036.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(4558..5310)
                     /locus_tag="ACG9BE_002313"
     CDS             complement(4558..5310)
                     /locus_tag="ACG9BE_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN3033037.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(5469..5678)
                     /locus_tag="ACG9BE_002314"
     CDS             complement(5469..5678)
                     /locus_tag="ACG9BE_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033038.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(5689..7017)
                     /locus_tag="ACG9BE_002315"
     CDS             complement(5689..7017)
                     /locus_tag="ACG9BE_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033039.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            7153..7308
                     /locus_tag="ACG9BE_002316"
     CDS             7153..7308
                     /locus_tag="ACG9BE_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFN3033040.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(7382..7969)
                     /gene="yihA"
                     /locus_tag="ACG9BE_002317"
     CDS             complement(7382..7969)
                     /gene="yihA"
                     /locus_tag="ACG9BE_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFN3033041.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(8052..9302)
                     /gene="clpX"
                     /locus_tag="ACG9BE_002318"
     CDS             complement(8052..9302)
                     /gene="clpX"
                     /locus_tag="ACG9BE_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFN3033042.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(9549..10838)
                     /gene="tig"
                     /locus_tag="ACG9BE_002319"
     CDS             complement(9549..10838)
                     /gene="tig"
                     /locus_tag="ACG9BE_002319"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFN3033043.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(10855..>11042)
                     /locus_tag="ACG9BE_002320"
     CDS             complement(10855..>11042)
                     /locus_tag="ACG9BE_002320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033044.1"
                     /translation="FKKKRKNFAANDLLPLDFISEKDKNVMKNKDSIHSICLQFERVS
                     VLFKGMHETFLMYKMNF"
BASE COUNT         3067 a         2297 c         1944 g         3734 t
ORIGIN      
        1 taaattaaaa gaaagcgagg cgtttgaatg gaaaaaaaag gaaaaatcaa gaaattagat
       61 aagactcaga tatattttat tctaaaagga atatttgttg gactgctgac aggaatcgtc
      121 gttagcctgt ttcgattatc tatcgaaaag ctaagcgatt tagttcgaag tatctatgaa
      181 atgagtcggg aacagccgat ttacttaatt ggcctagcta tttgctgcat ccttgcagca
      241 tttttcgtgg gttatctggt caaaaatgaa ccggatatca agggaagcgg gattccacaa
      301 gtagagggac agcttcgcgg ggaattatca atgaactggt tctctgtttt atggaaaaag
      361 ttcattggcg gtgtactttc agttggtgcg ggcctttttc taggtagaga aggaccttct
      421 atacagcttg gcgcatccat tggacaagga gctggacaat tgtttcggag tccatcttca
      481 gaagaaaaga ttttaatttc tagcggtgca agtagcggac ttgcagcagc attcaatgct
      541 cctatcgcag gattactctt tgttttagaa gaaatccatc actccttttc tccacttgtt
      601 tggctgacat catttgcttc agcaatcact gctaacttcg tctctcttta ctttttcggc
      661 ttgagacctg ttttatatct aggtgacctt ccttctttgc cgttgaaata ttatggttcc
      721 ttagtgatct taggagtact tctaggcatc ttaggtttta tttatcagaa ggtcttgctg
      781 gctttgccaa aattttacaa ccgattgcct ttaccagctt attttcacgg gttcgttcct
      841 ttcttgctga ttttgccaat tggttattta tggcctagta ctctaggcgg aggcaatcaa
      901 atcgttttag cttttggaca tgcttccttg ccaatcatta tgattatcgg tttgtttgtt
      961 cttcgattcg ttttttccat gatttcttac ggtggtggtc ttccaggggg gattttcctt
     1021 ccgatattga cattgggggc tttaatcggt agcttttatg gaaacattat ggttgatttc
     1081 gggatggatc caatctacat caaggatttt gtcgttattg ccatggctgg ttatttcaca
     1141 gcgattggaa aagcaccgct gacagcaatt attttagtga cagaaatggt tggctctctt
     1201 aatcatctga tgcctttagg attggctgct cttgttgctt atatcgtcaa tgaccttctc
     1261 ggcggtaatc caatttacga atcattattg gaacgtctac ttggaaatac tcagaccgcc
     1321 tcactaaaag gtgcaaggac aacttttgat tttccagtaa cggcagagag ttctttagac
     1381 ggattaatgg tacgagactt caattggcct aaaaatatgc tgctaatctc tatacgcaga
     1441 ggaaatcagg aaatcttgac tcacggtgat acgatcatga atgtaggaga tattttggtt
     1501 atcttgactg atgaagcaaa cttgccaaga atcaagcagg aaatcgaggc taaatcttct
     1561 atcacaaatt tgaagcaatc attttaacga acatggaaaa actattttac aacaactatt
     1621 catctacact acttgattct atatgattct ataaaaaaag aagagactgg gaaaaaccag
     1681 cctcttcttt tttatttcta tgcttctgct gctgcaggag cttttctcaa taaaccataa
     1741 atcacaccag atacgactgc tccgatagca ataaacacca aatacatgac cgggtgactc
     1801 agtaagaata caacgaaaat cccgccatga ggagccagta atttgatccc gaaagctccg
     1861 actaatccgc cagttaaagc agaaccaata ataaaacttg gaatggcacg aatcgggtca
     1921 gctgctgcaa atggaatcga tccttccgtg acaaacgata gtcccatcac gatgttcgtc
     1981 aaaccagcat cttgatctgt tttgctgaat ttattcttaa acaaacgtgt agcaacgaaa
     2041 attgccaatg gaggtaccat tcctccagcc ataacagaag ccatcacgac acttccgcca
     2101 gttgcaacag tcgtagctaa agttgctgta ccaaacacat aagcggcttt gttgattgga
     2161 ccgcctaaat ctgccgccat cattccagct aataatgcac caagtaaagc agcattcgaa
     2221 ccactcatac ttgtcaagaa accgttcaat gcatcattga ttgctttcat agggacattg
     2281 accagcaaca tcaagaaacc agtaatcagt aagccaaaca ctggataaaa gaggatcgtt
     2341 ttaattccat ccaatgattt tggtaagttc ttgaataatt tacgtaaaaa tacgatgaca
     2401 taaccagcta agaatccacc aatcagtgca cctaagaatc ctgcaccgcc tgtattagct
     2461 aatgcaccag cagcaaatcc aacgatcaaa ccagggcggt caccgatact tgaagcaata
     2521 aatcctgcta ataccggcag catgaaacca aatgcagctt gtccgatttg gttgaaccag
     2581 ctagcagctt gattgtaatt ccctagattt gataattgat catgtggcac gccaataaat
     2641 tggtcgatca tgaaagaaag cgcaatagcg attcctccac caataacgaa aggtaacatg
     2701 tgtgatacgc cgttcatcaa atctttgtaa attctttgtc cgatagtacc atccgcattt
     2761 ccttcttctg aactgctttc ttttccatca ccatggaata ctggtgcagt accactaagg
     2821 gctagattga tcagctcttc tggtttacgg ataccatcac tgacaggacg attgatcagt
     2881 tcttttccgt caaagcgatt catctctact tttttgtctg ctgcaacgat tacaccttct
     2941 gcacgggcga tgtcttcagc agtcaatcgg tttttgattc cttctgaacc gttcgtttcg
     3001 actttgatct ccacacccat ttctttcgct tttttcttca gagcatcttc tgccatgtaa
     3061 gtgtgagcga tccctgttgg acaagctgta acggcaacaa cgaattttct ttctggatga
     3121 gcagaagctt ctgcagcttg tgtttcttgt tctgcttttt cttctgcttc tttttgctct
     3181 tcagctgttt ggaacaagtc ttggacttct tctggtgtat ttgcttcttt taattttcct
     3241 acgaaatctg catcaatcag caatcgagat agtgctgcta atgcttgcaa atgtgtgtcg
     3301 tttgcgcctt ctggagctgc gatcatgaaa aacagataag ttggctgtcc gtctaaggct
     3361 tcataatcta ctcctttttg gctttttgcg aataaaactg ttgcttcttt aactgcactg
     3421 tttttcgcgt gaggcatggc gattccatca ccaagacctg tagaagtttg tgcttcgcgg
     3481 gctagaattc cttctttata tgtttcgata tcggaaatcc ggccggcatc aaacattttt
     3541 tggaccattt catcgattgc gcctttttta tcagtcgctt gcaaatccat gatcatcgct
     3601 tctttgacca ttaaatcctt gatattcatg attatcctcc ttgaaactaa tcaatttttg
     3661 tgatttcaac ttctggcagc aactcatcaa tcaattcgcg agctgccaag tcatcagaaa
     3721 aagctgttgc gcttccgcaa gcaactcccc atttaaaggc ttcgattgga tctttcgtac
     3781 ggctgtaagc cccaataaag ccggcaatca ttgaatcccc tgcacctact gaatttttca
     3841 atgggcgaac taatacattc gttttataag ttccttgttc cgtgaagagc aaagcaccgt
     3901 ctccagccat tgagatcagc acattctgtg ccccttcagc tagcaatttt ttaccatacg
     3961 gaatgatttc ttctacagat tggaaggttg tattgtaaag ctcagctaat tcgtgattgt
     4021 ttggtttgat caacaatggt ttcttaacaa gtgcattttt taggtcatca cctgttgtat
     4081 caatcacgaa atctgcacct ttttctcgaa tgatatcaat cagttcttga taaaatcctt
     4141 ttcgcaggct agccggcgcg cttcctgaca atacgacgat atctccttgt cctactgttc
     4201 ctaatgcttg cttcaactca tcgatttctt catctgaaat agatgggccc aaaccgttaa
     4261 tttcagtttc agtatctgat tttagtttga tattgattct tgtatcttct tttacggttg
     4321 tgaacaaaga ctggatttgt tctttttcaa gccacttcga aataaattcg ccagtgaacc
     4381 ctcctaaaaa ccctagtgct gtagattgag aatcgatccg tttcaaaata cgggaaacat
     4441 tgatcccttt tcctccaggt aacttgaagt cttccttcat tcggttcaaa tcgcctagtt
     4501 ttaacccatc tacgcgtacg atataatcaa ttgatggatt aagtgttact gtgtaaatca
     4561 ttgtattgcc tccttaattg ttgtcttttc acggaattca ttaacgaatt ctaatggaca
     4621 acgctcggtt atgatcgtgg ctgcttccaa aggtgcaaca gaggtgaagg acaactggtt
     4681 gaatttggta tgatcaatca atacaaatgt ctcttcggaa tttttcatgg ctaaacgttt
     4741 aacagctgcc tcttctgggt ccggagtcgt gaatccttgt tcaagatggg caccgttcat
     4801 tcccataaat gctttattaa aatgaaaatt ttttagttgt tctatcgcat tagatcctaa
     4861 aacagcatta gttgccagct ttatcgttcc acctaaaata atggtagcaa gctccaaatc
     4921 aactaaattt gccgcatgtt gaacggaatt tgtcactact gtgatctgtt ttccttgcag
     4981 aaaaggaatc atttctaacg tagtagagcc agcgtctaaa taaatgacat cttcgtcatt
     5041 aatggtttca gctgccgatt tggcgatttg ctgtttttcg tgaacgtttt tgagtgattt
     5101 ttcactcata ttctgctcaa aattcaataa ttgctctttc ttagctccgc catgaactct
     5161 ttctagaaac ccactattct ccagctcttg taaatcacgt cttatcgttg attcagaagc
     5221 attaaaaagg tttacaagat cttgtgattt cacgattttc tgctgttcaa gcaagtctag
     5281 gattttttgt ctacgttcct ctgtaagcat tttcacgatc acctcaatac aaataatagc
     5341 atattttttt tctactcgca atataaatca gtcaaaaacg ttcataaaaa ttataaatca
     5401 gtcaaaaaaa aataaaaaac cagtcaaaaa gactggtttt ttattttttt gtctatcatc
     5461 aagccgtttt atcgtttttt attttcgctt cttgtttttt tcatataaac agaagcaggt
     5521 tttctttcct tatgaagaag ataataataa agtcccatcc cgccaaacat gaccaaaaaa
     5581 ccgatgactg atcgaatagg aaaattagga acaaaaaaga gacagacgga taataatgat
     5641 gccggaacca ttattcgata atagagttgt ttatccattt tcttcacctc atattttttg
     5701 taaagtaatc aattgttcta attctcggta atctaaaaat tgcacacgca gatgctgctc
     5761 acctttcttg acatcgaatt ggatacttcg ccgctttttc tttattcgtt tgaaggtaac
     5821 atcagagagt ctatgtttga tttcctttaa cggtaattct gacagggatt ctgaaggaac
     5881 atagattctt aaggataaaa gatactcttg atatttttca tctgttacct ctacttctag
     5941 atattcccta atataataac aataagcttc gattccatta tccaaagcaa cagtcggatc
     6001 gataacttta tagcacatca tgcgcgagcg attctcgtca acccaaggaa aatacgcctg
     6061 agcatttagt gttccgcgaa cacgcctttc tttataatct ccttgttgaa tatcataact
     6121 ttttgttaca aaaaatgaag cattcttttt aggaacaggt tgcatttgtg cattctgata
     6181 ctggacagat tggatcgtat ggtcagtgta aatgtattca acgaatggcg gtttcaataa
     6241 accgacacca ttgtcccaag caagatgatc aaacccaagc tggtccctaa aataggattg
     6301 aaacatttga ttgtttggct tttgccagtt aaaggggtct cgataaagct ggatacatat
     6361 gacttcgttt tctaattcct tcactttttc tacttgttgg atgtcgagaa aaatcggttt
     6421 agggatgatt tgatgataat agcgactata atacatttcc caacacgaag tgaaacataa
     6481 accttggtaa aaaagatcgt atcctggaaa ttggttgcaa tcgactacta cttccccgtt
     6541 ttgttgtttc attttaggta aatgttcgat ttcatccttt ttctcaaaca atagccgttg
     6601 agagatctgt tttgtattgt gatagataaa ctcgctataa gctcgcatat aggcgaacac
     6661 cccatgagca atcatttttt tctgaatgaa gtctcgaatc aaaaattgct tttctttgaa
     6721 aacagagata gacaatacat tttttatcaa taattgatta tcttccaagc gaaaactaca
     6781 ttcgttctcg tcatttccta caactaacca actttcgtct cttaccgatg ctagcattat
     6841 tttttcttga agctttcgaa gatcatacgg atccgcttgt tgtgtgaaag agcggataaa
     6901 tgttggctgg atcaacagac gtgtcaaaat agcccaatcg cttatttcct gttttttttc
     6961 ttcttcattt aagtctgtga gctggatagt catcaataat ttatcttcgc caatcatttt
     7021 ctcacctctt taaaatcttt ttataattta attataacca acgataaaga aaaactaaaa
     7081 ctttatgtct tagaaaccaa tttggacgat tacatgatag actaagtaaa aagaaacaaa
     7141 gaggtgttta caatggggtt gaaatttgaa cgtacagata atttagataa acttttcgaa
     7201 tcatttgctg tcgatccgga aaaaacagtt gcaccaaaag aagagatcga tcgatttcta
     7261 aaaccacagc cacaaaatga aaagaaaaaa gacaagaaac aaaagtaagt taagctcaaa
     7321 aaaagactta ccagccaaac ttttatgaaa gagtttgact ggtaagtctt ttttattctt
     7381 tttatgattc aattgcttgt tcgattgctg cccacgcttc ttcttttccc tgttttgtaa
     7441 cggaagaaaa gagaataaac gtgtcattgc gatcaaaatc caacgctttt ttgatttcgc
     7501 tctcatgctt gttccattta ccacgaggga tcttatctgc cttagtggca acaacaatta
     7561 cagggatatc ataatacttc aaaaattgat acatctggat atcttcttga gaaggcttat
     7621 gacgcaaatc tactaaagaa acgaccgcac gtagctggtc tctagtcgtt aaatatgtct
     7681 cgatcatctt tccccatttt gctcgctcag ttttggatac ttttgcatac ccatatcccg
     7741 gaacatcgac aaaatgtaat gcgttctcga tcaaataaaa attcaatgtc tgggtctttc
     7801 ctggcttacc ggaagtacgg gctaaattct ttcgatcaat caatgtgttg ataaaagaag
     7861 atttccctac atttgatcgt cctgccagag ctatttctgg caattgtgta tctggatatt
     7921 gttttggtgc cactgcactt atgataattt cagcttgatg gactttcatg gtctctctcc
     7981 ttaacaaggt gatgaataaa gcaagcggct gtcgcaaaag ccaaaatgac ttttacggca
     8041 gcctcttttc atcaacccgc ttttttctct gaatctttat agattactgt tggctcacca
     8101 gacaattcag ctgcttcttt tgtgataatc actttttcga tgttttcatt tgaagggata
     8161 tcaaacatca catccatcat gatttcttca atgatggaac gaagtcctcg agcaccggta
     8221 ttgcgttcga ttgctttttt cgcaatggct ctcaacgctt caggttcgaa ttcaagttcg
     8281 gtattatcta gagaaagaag tttttgatat tgtttgacta gcgcattttt cggctcagtc
     8341 aagatccgta ccagatcatc tgttgtcaat ttctctaaag ctgccataac aggtaatcgt
     8401 ccgataaact cagggatcaa tccgaatttt agtaaatctt caggaatgat atgctgcatg
     8461 acactttcgt cttcagaaag tttttggttt tgtgtaccaa atccaatcgt cttttcgccc
     8521 atacgattct tcacaatcgt ttcgatgcca tcaaatgcac cacctacgat gaacaatatg
     8581 ttcgtcgtat caatctgaat aaattcttga tgtggatgtt tacgtcctcc ctgaggtggc
     8641 acgcttgcaa cagttccttc tagaattttc agcaaggctt gttggacacc ttcgccggaa
     8701 acgtcacgag taatagatac attttcgctt ttacgcgcaa ttttatcgat ctcatcaata
     8761 tagatgatcc ctttttctgc acgttccaca ttgtaatcag cagattgaag caatttaagc
     8821 aaaatgtttt ctacatcttc ccccacataa ccagcttctg tcaaacttgt cgcatctgcg
     8881 attgcaaaag gtacattcaa tgtttttgct aatgtttgtg ctaaaaatgt tttaccagac
     8941 cctgtaggtc cgatcaagca gatattgctt ttttgcagtt caacatcatc tgtgttttct
     9001 tgtgcttgct gattgacacg tttatagtga ttgtatacgg caacagctaa cgagcgtttt
     9061 gcgcgatctt gtccaatcac gtagttattc aatacttcaa gaatttctag tggtttaggg
     9121 acttcagtga attcgcgtac agcttcttca taaaattctt catcaatgat ttctttacat
     9181 aggtcgatac attcattaca gatgtagacc cctggaccag ctacgatctt tttcacttct
     9241 tcttgggttt tcccgcaaaa agaacagcgt acggtctcat tgctacttgg attgtcatac
     9301 atggtcttca ccccttaact tgcaaaaagt atgcaagaaa ctatcaaaga ctttacagtc
     9361 ttttactatc ataacatagt ttagtaaaaa gaaaaagtgt ttaacaaagg atcaactgac
     9421 ctataccttt gcatttttta ctcttaacaa aaaataagga actgtgacaa aatcgtgtca
     9481 cagtccctta tttcgaataa acaattttct agaagtagct tcttcagaaa tgatttatta
     9541 cttgatttct attctttcgc ttctgtcgct gtacctgtga tcaattcgat tgcttttttc
     9601 attgtcacat catgttccag catatcttct gtcaacacgc gtttgatttg ttcgattggc
     9661 atgttgtatg tttctgataa ttcttggatt tctgcattga tttcttcttc tgttgcttga
     9721 agattttcag cagctgcaat tgcttcgatc acaagatttg ttcttgtgcg catttctgct
     9781 tcaccttcga attgtttatg caagtcttct tctgtagaac cagttaattg atagtacatt
     9841 tcaggagaga taccttgacg ttgcatgttg ttcaagaatt cgtccattga acgatgtact
     9901 tcatcatgaa ccatcacgtg tggcaattca acgatttctg cgttttcaac agccatacgg
     9961 atcgctgctt catcttttgc atcgtccgct gctttttctt ttgtttctac taattctttg
    10021 cgatattttt ctttcaattc gtctaatgtt gatacttcgt catcaacatc ttttgcgaat
    10081 tcgtcatcta attcaggcac ttcttttgct tttacttcat ggattgtcac tttgaagact
    10141 gcttcttttc cagcaagatc ttctgcttga taatcttctg ggaaagttac atttacatct
    10201 agactttcac ctgatttatg accaactaat tgatcttcaa aacctggaat gaatgagcct
    10261 gaacctaatt caagtgagta attttcgcct tttccgccat caaacgcttc gccatctaca
    10321 aagccttcga agtcgattac tactgtatcg ccattttcag ctgcagcatc ttctttgata
    10381 actaattcag cttgtgcttc ttgttcacgt ttgatacgtg catccacgtc agcatctgtt
    10441 acttcacggt cttgtttttc aacaactaga tctttatatt gtcctaattt tacttctggt
    10501 ttcactgtta cttcagcagt gattacccag tcttgacctt tttccatgct ttcaacatcg
    10561 atttttggtt gagaaacagg atcgatgcca gcttctttga cagcagcttc ataagcttct
    10621 ggtaatacag cattcaatgc atcttcatat aaagcttctt caccgtacat acggttgaat
    10681 acttgacgag aaactttccc tttacggaat ccaggtacgt tcaagctttt tttcacttta
    10741 ttaaacgcag ttgttaatcc tttttggatt tcgtcttgcg caatcgtaaa ttttaaaaca
    10801 ccatcattcg tgcctttttt ttcaaatgtt gcagtcattt aattccctcc gagtttagaa
    10861 attcatttta tacatcaaaa acgtttcatg cataccttta aatagtacac taaccctttc
    10921 aaattgtaaa catattgaat ggatagaatc cttgtttttc ataacatttt tatctttttc
    10981 actgataaaa tctaatggaa gaagatcatt tgctgcaaaa ttttttctct tttttttaaa
    11041 aa
//
LOCUS       DAZAID010000093        10741 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000093 DAZAID010000000
VERSION     DAZAID010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.095"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..968
                     /gene="thiD"
                     /locus_tag="ACG9BE_002321"
     CDS             153..968
                     /gene="thiD"
                     /locus_tag="ACG9BE_002321"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFN3033045.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1297..2748)
                     /gene="cls"
                     /locus_tag="ACG9BE_002322"
     CDS             complement(1297..2748)
                     /gene="cls"
                     /locus_tag="ACG9BE_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFN3033046.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3089..5755
                     /locus_tag="ACG9BE_002323"
     CDS             3089..5755
                     /locus_tag="ACG9BE_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN3033047.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6119..6871
                     /locus_tag="ACG9BE_002324"
     CDS             6119..6871
                     /locus_tag="ACG9BE_002324"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN3033048.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6893..8047)
                     /locus_tag="ACG9BE_002325"
     CDS             complement(6893..8047)
                     /locus_tag="ACG9BE_002325"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFN3033049.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8232..10673
                     /locus_tag="ACG9BE_002326"
     CDS             8232..10673
                     /locus_tag="ACG9BE_002326"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFN3033050.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3499 a         1911 c         2305 g         3026 t
ORIGIN      
        1 ttttctgatt tattgcaaat gttttttcaa ataaatttga ttaccaagaa aaggtagtgg
       61 tatatttact gagggatact ttgaaaaaca tatgatagaa acagtaacag tattatgaaa
      121 aatcttttta taaaaatgga ggaatcgatt tgatggaaga tcagtatgta caagcaatga
      181 cgatcgcagg atcagatagc gatggaagcg cagggatgca agcagatatg catacattct
      241 ttacaagaaa tgtttacggt gtttctgtca tgaccgcatg tgttgcaggt aattcttacg
      301 gaatcggagc aagtgtgaca ttgccaacag attttatcga caaagaattt gaactgattg
      361 cgaaggactt tcaaatacgt gcagctaaaa caggtatgct agcagattca aagttgattg
      421 agacagttgt aaaaaattac caaaaatatg attttggacc attagtcgtg gatcccgtaa
      481 tcgtaacgaa acatggaaat cttttactag aagaatctgc tttgcaaagc ttgaaagaaa
      541 agcttgtccc tttggccgaa gtactgacac ctaattttta tgaagcagaa aagctgactg
      601 gacggacatt agcagctgaa gaagattttg tgcaagcagc aaaagaactg caagatatgg
      661 gtgcaaaaaa cgttatgatc aaaggacatc atgctaaaaa tgcgcagcaa gaaacagtca
      721 atgactacgt attgttcgaa tcaggaaaat cattttggtt aaatgcacca ttttatccta
      781 ccgaccggat caacggaact ggcgattcgc tatctgcatg tattgcagct gagttagcga
      841 aaggaaaaac agttgaacaa gcgatccgat tggcaaaaga ctatgtaaac cgtgcaattc
      901 gtgaagaaat aaatgtaggg cacaaatatg gccccatcaa tcattgggca gctggtgaat
      961 tagagtaaaa gaaagaagtt gtgaaaaagt tgccctgcat caagtaataa gaacagccaa
     1021 gtaaaaacag cttcttatgt ttttaacttg gttggactta ttaccgcaga tgcaagcttt
     1081 tgaacaccgt ttaagaaaga ggtcgtgagc ctgccgtcca gctccgagtc acaaggaaca
     1141 agttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttggc
     1201 aggagaacac cgtttgttcg gaagaaggga ctgtgacaag atttttgtca cagtcccttt
     1261 aatcttagta tatttttctc tacttgaata aaaaaattat aggattggag aaaataatcg
     1321 agaaatttgt tgcttgaata atagccagtt agacatttcc ttgactgttt cagttgtcat
     1381 ggatgaacaa tgtgtctcat cttctttgaa ctgagtagca agttcttcta aaaactcagg
     1441 atcgtaaatg acagcacttg tttcaaaatt caatcggtag ctacgaatat cttgatttgc
     1501 tgaacctacc atgcagattt catcatccat tatcaatgtt tttgcatgga ggaagccgtt
     1561 ctcatagaca aggatttgta cattttcctt gatcagctgc cgagcataat attgtgttgc
     1621 tcgataaata aaaggatgat ccggtttgtt gggaatcatg attttcacat ctacaccaga
     1681 ggcagttgcg atttttaaag cagcaatgac actttcatca ggaacaaggt aaggtgtctg
     1741 tatccaaaca cgttttttag cagaagtaat caatttgata aatgaaagct tgatttgttc
     1801 cctttcgtta ttcggtccgc ttgaaacaag ctggatggat gtatcagcaa gcttagattc
     1861 atctcgatct gcttttttaa agaaataatt caaatgatag ccgacctttt tttcttcagg
     1921 taccgagacg ttccagtctg ttaagaagcg aagctggagc aaagaagaag cggcaccgaa
     1981 aatccgtata tgcgtatcgc gccaatagcc gaattttttt gttgtattta catattgatt
     2041 ggcaatattg aaaccaccgg tataactaat ctttccatcg atcacaacga tttttcggtg
     2101 atcatggtaa ttcaatcgga aacgaagtaa tgccctttgt gaggtaacaa atgtatggac
     2161 atgtccacca tttttgatta gacgattgaa atcttttgct tttgtgccat gagagccaaa
     2221 tgcatcatat aatattcgaa cttccacgcc ttctgcggct ttttcttcta aaacatgtaa
     2281 gacttgctgg ccgatattat ctgtcacaaa tgcgtaatat tcgatatgga tcgaatgttt
     2341 ggcttttttt agatcttgaa gcagtgaatc caatttctct tgtccgtctg tgagaagagt
     2401 gacagaattc attcttgtca gcggcatacg atttaatgaa gagaagaagt caacaaattg
     2461 ctgtttgtct ttatcgccca tgtcaggatc gtaatgttca atggtatctc ctctaaaaga
     2521 ctgaaagttt tctaattctt tcaagtcact ttgtcggaga tagaattttt tcttatccgt
     2581 taatccacgt ccgaaaaata aatataatac aaagcccacc ccaggaaggg caaacaatac
     2641 taagagccat gcccaaatgg ctgctacatc tcggggtttg atcaaaatag ccaccaaagc
     2701 aataagtgca tttaatagat aaagggcggt tataatacta gataccacaa aaatgttcct
     2761 ccttacatac aataacatta gtatagcatg atagaagaaa gaagcctacc aaggtgaata
     2821 tacttttcac tcctttcatg aaaatgtagg taaatcgggc taaagtagtt gctttttgta
     2881 ggttaataag gtaggatatt agtacagttg aatatctcct taggggagta accggcaacg
     2941 tttgttgcga taatatcaac agtaagttaa ccaaatggtt ttctggtatt gtatgaaatg
     3001 gtgagaccta agacaaatgc gtatttgtct taggtctctt ttttttcaga caggactagg
     3061 gccaagatca aaaaatgggg gatgaaattt ggaaaacaag gaacaagcac aacaagcggt
     3121 ctataaagaa acgatcgact cgttgatgca gcaaacgcag agctcaaccg atggattgag
     3181 caccacgcaa gcgaaggaaa aattagaaag agatggcttg aaccagctaa aagaaaagcc
     3241 gaagaagcca acttggaaat tgttcttaga aactttcaaa gatgcaatgg ttatcgtatt
     3301 gctgattgtc gcactgattc agatggtcat gggtgactac atcgaattct tagttatttt
     3361 tgcagtattg atgttgaatt ctataatcag tgttgtccaa acaaaaaaag cagaaggttc
     3421 tttagaagcg ttgaaaaacc tttcagcgcc cgatgctcga gtgattcgtg atggtaagga
     3481 aaaaacgatt ccggcaaacg aacttgtggt aggagatatc gtgttgttag acgcaggcga
     3541 ttatgtacct gctgatggac gtttactaga tgcgggatca ttaaaagtag atgaaggtat
     3601 gctgacagga gagtcgactt ctgcagaaaa agaagtaacg gatatcacaa gtacggtacc
     3661 aatcggagat cgtacaaata tggtattcag tggaacgatc gtaacttacg gacgcggtaa
     3721 attcttagtg acttcaactg ggaataatac ggagatcgga caagttgctg gccttttgga
     3781 atcaacaact gagcaagtca ctccgttaca acgaggtctg gataaattca gtaaaaaact
     3841 aagtttggct attttggcat tgtctttatt gattttaggt atccaactgt ttcgtattta
     3901 tcttggagaa ggtacaggag atatgactgc agatatcgtc agcgcagtga tgtttgccgt
     3961 agccgtagcc gtagctgcta ttccagaagc attacagtct atcgtaacaa tcgtgttatc
     4021 tttgggaacg aataaaatgg caaaacgtca tgccattatt cggaagcttc cagcagttga
     4081 aacacttggt tcgacaagta tcatctgtac agataagact ggtacattga cacaaaacaa
     4141 aatgacagtt gttgactact ttttaggtaa tggcgatact ggggatttca caaatgaccc
     4201 ttcaaaatgg acagctgacg aacgtcgctt gatcgaaatc agtgttttag cgaatgatgc
     4261 atcgatcagt gaagacggta ctaaattggg cgaccctacg gaagtagcgt ttatcgactt
     4321 tagtgaaaaa ttaaaccagc cttatcagga aatccgaaac aactacccaa gacaagcaga
     4381 acttcctttt gactcagatc gaaaattaat gtctactgga cacacaattg ataacaaaaa
     4441 ttacttgttt gttaaaggcg gaccagatat cgtcttccaa cgcagtaaaa aagtattgct
     4501 aaatggtgaa gtcgttccga tgacggatga attgatgaaa aaattccaag agcaaaatga
     4561 agctttttca acacgcgcat tacgtgtact agcttttgct taccgtccac ttgataattt
     4621 tgatttatca tttgaagatg aagacgattt gatccttgtt ggtttgcttg ctatgatcga
     4681 tcctccacgt gctgaagtga ctcaggcggt aacagaagca agaagtgcag gaatcaaaac
     4741 aatcatgatt actggtgacc acaaaacaac agctgttgct atcgcaaaac aaatcgggat
     4801 ttttgaagaa ggaaacctgg ctttgaccgg gacggaacta gatgaattga cagaagatca
     4861 actaaacgaa gttttagaaa aagtcactgt ttatgcacgt gtttcacctg agaataaaat
     4921 tcgtatcgtt cgtgcttggc aaaataaagg tcatatctca gctatgacag gtgatggtgt
     4981 aaatgacgca ccagcattga aacaagcaga catcggaatt gctatgggaa cgggtacaga
     5041 tgtggcaaaa gatgcagcag cgatggtatt gacggatgat aactttgctt ctattgtcag
     5101 cgcagtagaa gtaggacgta atgtgtttga taatatcaag aaagctattt cttatctatt
     5161 tgcaggaaat ttaggtgcga tcattgcgat catctttgcc ttagttgctg actggtccaa
     5221 tccatttaca gcattacaac tgttgttcat caatttagtc aatgattcac tgccagcaat
     5281 tgcattaggt acagaaaaag ctgaacctgc tacgatgaac cgcccgccaa gagatccaaa
     5341 ttcaggaatc tttacggaca aaacattgat ttctgtaacg tatcgtggtg tgctgattgg
     5401 gattgctgtt atctttgctc aatggcttgg catgcagcat tcacctgaat tcggtgtagc
     5461 catgtcattt tcaacattga tttggtgtcg gacattgcaa acattcccag cacgttcaaa
     5521 cacacaaaca gctatccaag cagggttctt ctctaacaga agtgtcttac tagctgtagt
     5581 tgtatgtagc gcgctttact gcttaacaat gatcccagga ctgaaagaag tcttctctat
     5641 cccatcagca tttggattga cagaagtctt tacatgtatg ggaatcgcac ttgtgacaat
     5701 tgttttgatg gaaatcacta aattgatcat tgtacgagta caaggaaata aataaaacga
     5761 atcaacgagg ttgtgagcct gccgtttaat tctgagtcac aaggtgcaat ttcaacaaac
     5821 agcttctcat gtttgttgaa tattgtgact tgtgaccgaa gagttggcag gagaacaccg
     5881 tttaaggaag aagttgtaaa atcagcattt tgagctaata ctgagggtca tctgattgga
     5941 cactgtttat tcggaaagag ggactttgac acgactttgg tcacagtccc tttttttatt
     6001 tatgatagac ggaaagccaa tcattttatt attcatcaaa agagatagaa tagataaaag
     6061 aggagaatga tttattccga aaagtgaaaa catataacaa aaaattagag gaaatcaaat
     6121 gaatgaaaaa gaatgggatg aatttgctga agaatattat gaaatccagc aagaatcgca
     6181 agtaactatt gctgaagatg taaaaaatta tctgaaaacg gagaaagtcc tgcctgctga
     6241 aacagcagca gacgttgctg gaggatctgg cagatttctt tccttagcaa aagaaattaa
     6301 tcgctatgat ttgatcgatt tttcttcagg aatgctagaa aatgcaaaaa aagaagcaga
     6361 aaaaaatgag ttgccaaatg tttcctttat caaacagtcg ttagaagaat ttatccaatc
     6421 aaatgagaca tatgaaatgg tttttacagc agcaaatcca gcattggtac atcttattca
     6481 attagaaaaa ttgatgaaaa agtcaagcaa atggtgcgtg ataatcagag tagtggaatc
     6541 aatcgatgat gttttttatc ctatcgaaaa gcaactagga atgatagaag aagatcctaa
     6601 tacatcgcca gttgtaatgg atcaatttga aacatactta attgatgaag gattcaaagt
     6661 gaagaaaaaa gattttactt attttacaca ggaatcaatt actgaagaat tgatgaagct
     6721 atactatgac gcgtacgcaa aagatcctgt tgtgaccagg tacattcgca aactattcag
     6781 tgaaaaacga gaaatcctta gtacgactaa gctgacctat agactgctcc tttggtccgc
     6841 agataaaaaa agaacctttc acgactcgtg aagggttctt tttatgattt tattatattt
     6901 tgtagtaacg aatatcattt tcgatctttg taatcgaaaa aggtacatct gaagtatatt
     6961 ctgcacattc tccgtgttca ggaagcgtct cactgaaaat cgtttcatat tcgtcaacag
     7021 tgatccgtgt tcgagaatct agcatctctt gatgagattg tgcgaagaga tattcttgat
     7081 agttttcttc cagataacct gtaaaaaatt cacttactgc accagaaccg tagctgaata
     7141 gaccgatgcg atctcctggc tgtagtgatt tcgaattttc cagtagagaa gttagcccca
     7201 ggtataatga accagtgtaa agattaccga ttcgtcggct gtaacggatg ctttcttcat
     7261 agcgagccat aagacgttcc tgattttctt cgtctgtttg gtctaatacg ctttgcaatg
     7321 ccttttttcc catcttagta tacggaatat ggaaagcaat cgcctgataa tcttcgagtc
     7381 ctcgacccga caattcttta tgtcgattcc aaactttttg gaatgattcg atatacgtag
     7441 aattagataa aggaccatca acaacaggaa attcgctata atctggacgc cagaaatcat
     7501 agatatcttc tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc gggttttgag
     7561 tgatcatcat cgcaacagca ccgacacctt gcgtcacttc accaccgctt gccaagctgt
     7621 aacgagcaat atcacttgct atgactagta cttttcgttc tggatgattt ttgacatatt
     7681 ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc ctcttttact tcaaaagaac
     7741 gagcaaaaga ttggattttc agtaaatggt ggataattac cgcactcgct tttgaatgat
     7801 caatcccgga ttctgttccg acgatgacca tgtcgattag ctcacggtct ttttctgtta
     7861 cgattttgct ggcagcattt gctcctagtg taatgatgtc ttcatttgca cgattgactg
     7921 ccatttggtc ttgaccaatc cctatatggt attttgcagg atcatcccca cggctttctg
     7981 ccaactctgt catatctaaa tataaattag gaataaaaaa ggaaagacga tcaatcccta
     8041 ttttcatttc aacacctctt tacttatgat aaaaaactta ctttactaag gtatcataaa
     8101 aagattaaaa gaaatataaa aaagagaata tttaaggatt gttaaaattt aatcttagaa
     8161 aaaaataact ggaacaagag ctatttgttt ttacagcaaa gaatggtata tttttgtagg
     8221 aggtggctta cttgaaagaa gtcgttatga tagatgctgc aagaacgccg ataggaaagt
     8281 atcgcggaag tttaagtcca tttactgcag ttgaattagg cacacttgtg actaaaggat
     8341 tattagataa aacaaaactg aaaaaagata aaatcgatca agtaatattt ggaaatgtcc
     8401 tgcaagctgg taatggtcaa aatgttgcca gacagattgc tttgaacagc ggtctccctg
     8461 tagatgtccc agcaatgacg atcaatgaag tttgcggatc tggtatgaaa gcagtgatct
     8521 tagcacgcca gctgatccaa ttaggtgaag cagaacttgt gatagccggc ggaacagaga
     8581 gtatgtctca agcacctatg ctgaaacagt atcagtcaga aacaaatgaa tatggtgaac
     8641 caatttccag tatggtcaac gacggattga ctgacgcatt ttcaaatgca catatgggat
     8701 taaccgcaga gaatgttgca acacaatttt ctgtgagcag agaagagcag gatcgctatg
     8761 ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt tctgaggaga
     8821 tcatcccagt caagatttct gatgaagacg tgttatctga ggatgaagca gttcgtggaa
     8881 atagtacatt ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa ggaactgtaa
     8941 cagcaggaaa tgcttccccg ttgaatgacg gtgcctctgt ggtgatcctt gcatccaaag
     9001 aatacgcaga aaataataat ctgccttatt tagcaaccat caaagaagta gcggaggtcg
     9061 gtattgaccc aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa ttgacagata
     9121 gatcaggaat gaatttatca acaatcgatc tttttgagat caatgaagca tttgcagctt
     9181 cttctatcgt agttagccaa gaactgcagt tagatgaaga aaaggtcaat atatatggag
     9241 gagcaatcgc attaggccat ccaattggtg caagcggtgc acgtatcttg actacattag
     9301 cttacggatt gcttcgtgaa cagaagagat acggtattgc ttcattatgt attggtggag
     9361 gtttaggctt ggcagtcttg ttagaagcga atatggagca aacacataag gatgtgcaaa
     9421 aaaaaaagtt ttatcagctt acgccatctg aacggcgcag tcaattaata gaaaaaaatg
     9481 tattgacaca agaaactgcg ttgatttttc aggaacaaac actttctgaa gagctctccg
     9541 atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc aatggggatc gcgcagaatt
     9601 ttcaaatcaa tggcaagaaa aaatggattc cgatggcaac agaggaacca tctgtcattg
     9661 cagctgcaag taatggtgca aaaatctgtg gaaatatctg cgcagaaaca cctcagcggc
     9721 tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg atcaatgcag
     9781 ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga aagttatcct tcaatcgtga
     9841 aacgcggggg cggtgttcaa gatatctcta ctcgggaatt catggggagt ttccatgcat
     9901 acttatctat tgatttttta gtggatgtaa aagatgcaat gggcgctaac atgatcaact
     9961 cgattcttga gagtgtggca aacaagttac gggagtggtt ccctgaagaa gagattctat
    10021 tcagtatttt aagtaatttt gctacagaat cattggcttc tgcctgttgt gaaatcccat
    10081 tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa atccaacagg
    10141 caggggaata tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa gggatcatga
    10201 atggtataga agctgtagtg gcagctacag gaaatgatac acgtgcggtt agcgcttcga
    10261 tccacgccta cgcggcaaga aatggattat accaaggatt aacagactgg cagatcaaag
    10321 gggataaact agttggaaaa ctaacggtgc ctttggctgt tgccacagta ggcggagctt
    10381 ccaatatcct gccaaaagca aaagcatctt tagctatgtt agatattgat tcagccaagg
    10441 aactagcaca agtaattgct gcagtagggt tagctcaaaa cctggctgct ttgcgtgcat
    10501 tagttacaga aggaatccaa aaaggccaca tgggacttca agctcgttca ttagcgatct
    10561 caattggggc tattggtgag gaaatcgagc aagttgcgaa aaaattacgt gaagcagaaa
    10621 agatgaacca gcagacagct atacaaatat tggaaaaaat ccgggaaaaa taaaaaagca
    10681 atatctttag aagaataaag caagatttac agttatttta tttactgcag atcttgcttt
    10741 t
//
LOCUS       DAZAID010000094        10670 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000094 DAZAID010000000
VERSION     DAZAID010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.096"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(188..1036)
                     /locus_tag="ACG9BE_002327"
     CDS             complement(188..1036)
                     /locus_tag="ACG9BE_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN3033051.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1200..2102
                     /gene="gnd"
                     /locus_tag="ACG9BE_002328"
     CDS             1200..2102
                     /gene="gnd"
                     /locus_tag="ACG9BE_002328"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFN3033052.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2104..3105
                     /locus_tag="ACG9BE_002329"
     CDS             2104..3105
                     /locus_tag="ACG9BE_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFN3033053.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3102..3386
                     /locus_tag="ACG9BE_002330"
     CDS             3102..3386
                     /locus_tag="ACG9BE_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFN3033054.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3419..4216
                     /locus_tag="ACG9BE_002331"
     CDS             3419..4216
                     /locus_tag="ACG9BE_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN3033055.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4230..5063
                     /locus_tag="ACG9BE_002332"
     CDS             4230..5063
                     /locus_tag="ACG9BE_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN3033056.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5081..5938
                     /locus_tag="ACG9BE_002333"
     CDS             5081..5938
                     /locus_tag="ACG9BE_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3033057.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5960..6469
                     /locus_tag="ACG9BE_002334"
     CDS             5960..6469
                     /locus_tag="ACG9BE_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN3033058.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6506..6907
                     /locus_tag="ACG9BE_002335"
     CDS             6506..6907
                     /locus_tag="ACG9BE_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033059.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7019..7372
                     /locus_tag="ACG9BE_002336"
                     /pseudo
     CDS             7019..7372
                     /locus_tag="ACG9BE_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7406..7849
                     /locus_tag="ACG9BE_002337"
     CDS             7406..7849
                     /locus_tag="ACG9BE_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3033060.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7866..8951
                     /gene="uxuA"
                     /locus_tag="ACG9BE_002338"
     CDS             7866..8951
                     /gene="uxuA"
                     /locus_tag="ACG9BE_002338"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN3033061.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8944..9657
                     /locus_tag="ACG9BE_002339"
     CDS             8944..9657
                     /locus_tag="ACG9BE_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3033062.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9864..10013)
                     /locus_tag="ACG9BE_002340"
     CDS             complement(9864..10013)
                     /locus_tag="ACG9BE_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033063.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10409..10609
                     /locus_tag="ACG9BE_002341"
     CDS             10409..10609
                     /locus_tag="ACG9BE_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN3033064.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3561 a         1690 c         2314 g         3105 t
ORIGIN      
        1 gttatcgcat ctaacaagta actcgaataa taccaataca accttctcca aagatgcgat
       61 gacatacgtt ctaacccacc gactattgat gttctgtacc acccttgcag aaccaaaaaa
      121 gccactacca aatatttggt agtggctttt tttgttttat ggatttttcg ctttttctgt
      181 tatggtgcta ttctttgatt tcagaaatag tatgtcgaac tttagcgata gaacgacgtg
      241 attctttttc gttgtaaaac atcaaaatca catatagaga gtccaaaatg cttagttgag
      301 aaatacggga agctaaagct tcagatcgga attctgtttc ttcagaaata gaaataaaaa
      361 cgacatcgcc aagtttagca agggaagaat tagcttggct agtaataacg attgtttttg
      421 ctccagtttt tcttacggtt tcagcaatat ggattgtttc ttttgattgc cctgtgtgag
      481 aaatcaaaac agcacagtct ttttccgtca ataaagaagc ttccatcaat tgaatatggt
      541 aatccgtgct gtgagaagtt gcgatagggg aacgcaaaaa tttatgataa gcgtcagttg
      601 ctacaatctc tgaaccgcca acaccgaaaa aatagacacg tttagattca ttgataatag
      661 tagccgcttt ttttagatcc ttttgttgta acaacttttt tgtgtcattt aaagttgaaa
      721 tactagactc gaacaccttt tgcgccatag tgagctcagt atctgttttc tgaatatttt
      781 cgtggataga aatagcagat agatcatttt cttggatcaa catggccatt ttaaaatctt
      841 taaatccatt ataaccaagt ttttttgtaa attgaaaaaa agtagagtca gcaatgccca
      901 gttcacttgc taaattgcta ataggactat gacttacttt gcttggattt tctaaaatat
      961 aatcggcgat agcctgttct tttgggctga agtcgttgta cattgttttg atttgtagtc
     1021 ttatggagac gctcatgaga attcctcatt ttcataataa gattatcatt ttttccgttc
     1081 tcattaatgt taaagataca aggagagtag gaaaaaatca aacaaaaaat atttttgtaa
     1141 aatctattta caaaaaaatt tttttgtata atgagggtat aagaaatgga ggcgctatca
     1201 tggaaattgg tgtaattggt ttgggaaaaa tgggattgaa tttagcttta aacttaaaag
     1261 agcaccatca caaagtacaa ggactcgaca tttcaagtac agctgttagt caggcaagag
     1321 aaaatgacat tcctgcttat caagagatct ctgaatttct agctactttt gagcagaaaa
     1381 aaatcatctg gttaatgctt cctgctggag agataaccga aaacatgtta agaaaacttt
     1441 ttccaaaact cggaccagga gacattatta ttgaaggcgg aaactcttat tataaagatt
     1501 caatccgtcg agcagaagag tttcagaaaa atgaaatcgg ttacttggat tgtgggactt
     1561 ctggaggaat cgaaggagca cgacatcaag cctgcttaat gattggagga gataaagaaa
     1621 cttttctatc cgtggaacaa ttattcaaag atgtagcaat tgaaagagga tatctgtatg
     1681 ctggtgcatc cggaagcgga cacttcttga aaatgatcca taatggaatc gaatatggaa
     1741 tgatgcaagc aatcggtgaa ggattccaac ttgtacaaaa aagtgagtat gcttttgact
     1801 tggaacaagt cgcacgtgtt tggaatcatg gttcggtgat tcgcggctgg ctgatggaaa
     1861 tcatggaaga tcagttaaac gaacatccca atttaagtaa ttatcgggga atcgtttctg
     1921 catcaggtga agcaaaatgg acggttgaaa cagcgctaga aatggctgtt ccagttccag
     1981 taattgcaac tagtttgttc atgaggaatc tttcacagga agaagatagt ttttcagcaa
     2041 aagtagtttc agcactaaga aatgggtttg gcggacatga aatagttgaa aagggtgagt
     2101 aaaatgccag attatcatat agcaatcgtt aattccagca gtttcggaag agtctttcca
     2161 caccacttgg aaagattaaa gtcagttgga gacgttcatt tctttactgt tgatcaagag
     2221 attccaggaa aagaactagc ggagttactt aaaggattca atatcattat tgcaagtgtt
     2281 acgccatttt ttcgacgaga tttttttgaa aacaaagatg aactgctatt gatttcccga
     2341 catggtattg gttacaacaa tattgattta aaagcagcaa aagaacacca gacaatcgta
     2401 tctatcatac ctgctttaat tgaacgaaat gctgtagcag aaaataatat aactaatttg
     2461 ttgtccatgc ttcgacgcac gacacaagca caagcaagag tcaaggacga tcgatgggaa
     2521 gatcgggcgg agttcgtcgg taggacgctg tttaacaaaa cagttggtgt gattggtgca
     2581 ggaaataccg gaagcggagt aatcgaaatt ttgcgtaacg gttttcgctg tgatgttcta
     2641 gcttatgatc cttacaaatc agatttatac atcgaaagct ttggtgcaaa aaaagtagaa
     2701 cttcaagaat tactggaaca atcagatatc atttgtttat gtgcgaattt gacagaagag
     2761 aactatcaca tgatttctga taaagaagtc gatcagatga aagaaaatgt ttatctatct
     2821 aatagtgcaa gaggcgctct agttgatgaa gaagcgatga ttaatggtct gaaaagcgga
     2881 aagatcgcag gatatgccac agatgtttta gaagaagaac cgggacgtca agatcatccg
     2941 tatttagcgt ttgaacaagt agtgattacg ccacatatat cggcctacac aagggaatgt
     3001 ttagaagcaa tgggtgaaaa atgtgtcaga gatgtcgaac agatcagtca gcaccttctt
     3061 cctgaacgtt ctgtccagac aaagagcaac tatataaaag gatgatgaag aaaatggtga
     3121 acggtttaaa agtgaaaaca ggccctcaat tttatttata tgaagaaggt ggtatttcaa
     3181 aagtttctga tctcttgaaa tcatacggcg cgaaaagagt actggtcacc cacggaacgg
     3241 tatcatggga aaaggcgcta cctaaattag tatttttgaa tgacgaaacg attcaatttt
     3301 tttatcatcg ttattctgga gaatgtagtt atgctgaagc cagacgaata gctacaatca
     3361 tcaaaaaaat gaaatcgatt tcgtagttgg cgttggcgga ggaaaactga ccgatctagt
     3421 gggttatagt gccaatcttg caagaatcga tttcggagtc atccctactt tagctagtaa
     3481 ttgtgctcca tggacacctc ttgctgtcat gtataaggag aatggacaag cagaagggaa
     3541 aacagaacac ttttacagac aagctgcttt ttttataaca gatccccaat tagtgattga
     3601 ttcccctgtt ccatatttca ttgccgggtt agcagataca ctagcaaaat ggtatgaatc
     3661 tgagctcatc ttagaacaag attttttgca agatgaatca tttttgaagc tagcacaaga
     3721 tactgcgaag atctgcaaag aagagatttt gaatcattct gctaaagcaa tccaagatat
     3781 ggcacaaaga aaactgacat cggagttcaa acagctttca gagattatct ttgctgttgc
     3841 tggtttagtt ggaggattag gagataaata cgcaagaaat gcggcagcac atgctatgca
     3901 tgacgctatc agtaaatata ttccggaaag ccatcgtttt ctacatggag aaaaagtagc
     3961 gtatgggatt ttctatcaac tggcactaga aggtcgctgg gaaaaaatag acgagttata
     4021 tcctttgtat cgggaattag aactgccggt ttctctagaa caaatgggat tatttccgaa
     4081 agataaacag ataatagata aaatggctga atttattgat tcgaaagaga aagtgcattt
     4141 gattcctata gaggtaacac agaaatcact gatacagtca atgatggaac tggaaggtta
     4201 tatcagaaag atctgaagga ggaggacaca tggaaacatt aagtgtagta caaagtttgt
     4261 tgattgcttt atgggtagcc gcggttatgt cccgctggtt aggtggagga gccacgttga
     4321 ccttacgttt ttctcctttg atgactggat tagtcgtcgg gttgatcatg ggggaagttg
     4381 cacaagcgat gattgttaca gccgctttgc aaatgattta tatgggggtt ttttctccag
     4441 gaggatcaat gccagctgaa ccatctattg cagcagctat cgcagtacca gtcgcattat
     4501 taggggatct gtcgccagaa gcagcgattg ccgttgcggt gccagtcgga ttgctcggaa
     4561 gttacttgta tcagtttcgt tttttcatca atactttttt aggtaaatat acagacaaag
     4621 ctgtagaaga tttgaataca aaagctatgg cccgatcaat tattctttat ccaacggttg
     4681 cctcattttt attattcgtt ccgcttgttt tcattgcttt atatttaggg gcgccattga
     4741 tcgctgatgt aatcaaagct ttagagggaa cagtcgttat ccacattctt gaagtagtag
     4801 gtggagggtt agcggcaatc ggtatcgcga caactgtata cgttatcggt cgaaaagatt
     4861 atctggtctt tttcttcctt gcttatttta tgagtgttgt tttgaaatct ttagatatta
     4921 cgatggttac ttatgccatt tttgggatta ttatcgcttt gatctttgtc cagtcgcaaa
     4981 aagggaaaca agtacagcct gccacaagca ataacaattc agcatctttt gatgacgaag
     5041 atgactatga tgacggattt taatttagga ggaatgggaa atggctgaaa taatggataa
     5101 tcaaacaggt gctcctgaag aaatcacgaa aaaagatgta acaaaagcct atctgagatg
     5161 gcattttgca aacgaaattc ctcactcttt tgaacgttac ttggcgccat cactactata
     5221 cggaatgatg ccaatcttga aaaaacttta taaagatgaa gactctttaa aagcagcata
     5281 taaacggcag ttactgttct tcaacactca attaagctgg ggtggcggtg tgattactgg
     5341 cctaatgtct tctatggaac agcaaagagc agaagaagag tatgaagaaa aagagatctt
     5401 gatgcaggat gacttgatgt ataacacgaa agcgggattg atgggggcac tagcaggaat
     5461 cggagatgca atcgattcag gaaccgtcca atatattttt atcgcaatcg cggtaccttg
     5521 ggcacaacag ggaagtcctt taggcgcact ttttcctttt atctgctttg ggttgtatca
     5581 ggttttacta ggggtatttt tcgcaagaca agctttcact atgggccgta atgctacagg
     5641 cctaatgcaa aacactggtg ttcagacagc gattgaaatg ctatcagttt tagggttgtt
     5701 tatgatgggg gtgcttgcgg gaaactatgt aaaagtagaa tcttcattga aatttgcgat
     5761 atccggacgt gagtttgtta tccaggaaat tcttgatcaa atcgtgccag ggatattacc
     5821 tttagctgtc gtaatgggtg tgtacttgtt ctatacgaaa aaaggattga aagtcacaca
     5881 agcattatta tggctgactg gaatattaat tgtgctagca ggaatcggta ttttataaaa
     5941 agaagggaac gtgaataaga tgggtgtagt caatttagcg cgtgtggatg agcgtttgat
     6001 ccatgggcaa gtaatggtaa cgttatctca aaaaagtgga gtgaattcca tttttgttgt
     6061 agacgaagtt gtagcaaaag ataaatttat gagagatctt tacaaaaatg caggaagccg
     6121 tacaggacaa aaaacaattg tcattacacc agagaaagct aaattctatt gggatgaata
     6181 tcaatttaaa gaatacaatt gtattttgat cgcaaaaaca gtccaaacga tttacgactt
     6241 agtaaaacac ggaatcccca tggaagaatt gaatattgga gggattgctc aaaaaaatcc
     6301 tgatacagat cttttagtga cgaaatctgt ttatttaaat aaatcagatg ctgaaaaatt
     6361 gaaagaaatg aatgaacaat atggcgtaaa gaatatttat tttcaagcca ctccatctgc
     6421 acctaaaaca ggattaaaag atgtattgaa caagtttgat ttgtcataaa caaatgctta
     6481 gaaaaaagat aaagagggcg aaaaaatgaa gaagaacgag ctgtttcgtg attgggaatt
     6541 tcgttatcgc tatatttacc gtaagcgccg tacaaaaaag agtaaacagc gttttctttc
     6601 agcattagta tcagatattt acagtatgag aacagatgtg acagtcattg cttacgatac
     6661 gctagcctac cgatcaaaaa atatttatgt tggagatatc gaaaaagcag agaaagttat
     6721 ctgtacttat tacgatacac ccgttcatgc attaggaagt tatttcatgt ttgattggaa
     6781 agaccaaagg aaaaaaacaa tctactctat attattgtcc ttcattttgt tgttcagttt
     6841 aggttggtgg ggaatgatga tatataacaa aaatcctcat cacgtattcg atctgctatc
     6901 cgtataaaca ggtatcactg tactagcatt tgggagctac tttttcttat tgggaaaagc
     6961 agctcgcgga tggcctagca gacaaacttt tattcgaaat acttcatcca tattaacgat
     7021 gctagaaatg atccgtacga ttgatgatcc gagtgtagcg tatgcgtttg ttgatgaagg
     7081 ctgttatgga gaaaagggct tagacagcgt gagatcgggt atgaaaaaag aggctattct
     7141 tttttatcta gactctgttg gggcagatac accgctacag ttttcgggaa attatttttc
     7201 aaataaagaa cagtgactga aacaagttga taagttgaaa gaaaaaaacg tcaattatat
     7261 ttttagtgca agaaaaaagc aagcgcagtt cttctatttg accaaaacag atttacgagg
     7321 gaaaacattt aattggcaaa atgccaatca gattattgct ctttttcgat aagtaaaaag
     7381 tgcaaaacaa ggagtgagaa gactaatgtt ggaaatcatg atcgttacac atggtaagtt
     7441 gagcgaagga ctgaaagatg cggcggaagt aattgtcggg acatcgagcc atattgaaac
     7501 aatgtgctta gagtctgggg aggacatcca aacattcgga gagaaggtca aagaaaaatt
     7561 aactatgatc aacacagcag aacccatgct agtatttgtt gatctaatta gcgcaagtcc
     7621 gtacaatcaa gcgctcttgg cagtcaatga actgactgaa gaaaagaaag cagccactta
     7681 tgtcataggg ggcgtgaatc tgccaatgtt gttagaagca atcaatcatc agatgcttca
     7741 cacaccgata gaagaagcgg tggaacaaat tgtcaaacaa ggaaaagata atttaactgt
     7801 ttggcatgct tcagcggaag aaaaaaaatt agatgaggac gatttttaga aggaaggctg
     7861 aaaagatgaa gtggggattt agatggtatg gtgaaaaagg ggattcgatc ccattaaaat
     7921 atgttcgcca aataccggga ataaatggga taattggaac tttgctgact aaaatgcctg
     7981 gagatgtttg ggagatttca gaaattgagg agttgaaagc atctgttgag agccaaggac
     8041 tggaactgct tggaattgaa agtgtagcga tccacgatgc aatcaaggca gggacagatg
     8101 aacgagatta ctatattgat aactatatac gaacaatcaa aaacttgtct cgatgtgggg
     8161 ttcgattgat ttgttatagc ttcaaaccaa tttttggatg ggcgaaaact gatttgttta
     8221 atgaaaacaa cgatggaagc tattctcttt tatacgatca gaaaatcgtc gatcagatgg
     8281 agccaggaga aatgtatcaa ttgatacaca gccagtcaaa gggattcaaa ttgccgggtt
     8341 gggaagaaga acggttgaaa aaattcgctc agctggaaaa actgtatgaa ggaatgaccg
     8401 aagaagaatt atttgaaaat ctgacttatt ttttagaaaa agtgattcca gtgtgcgaaa
     8461 aagcagatgt aaaaatggct atccatccag atgatccgcc atgggaactt tttggttttc
     8521 ctagagtgac gaaaaatctt ggcgacttga agaaaattct aaatgcagta gattctccat
     8581 ataatggact aacgctgtgc acaggatctt taggggcaaa tccggaaaac gatttagttg
     8641 aaatcatcca tgaggtaggt tcgcgcatcc attttgtcca cttcagaaac gtggagtttt
     8701 taggagaaag aaagttcaaa gaaacggcac atccctctac agaaggctcc cttgatatgt
     8761 atcatattat gaaagcgttg gtgcaagaag ggttcgacgg tcctatacgc cctgatcatg
     8821 gtagaaccgt gtggggtgaa gtggctatgc ctggatatgg cctgtatgat cgtgcaatgg
     8881 gactcactta catgcaaggt ctatatgagg cgataagtaa ggaaaaacca ctgggagagc
     8941 attatgaata aaatcaagct cttcatcttt gatatggatg gccttttatt agaaactgga
     9001 cgtttagctt atcgtgctta tgtaaaatca gcacaaaagt atgattatga aatgcgtaag
     9061 gaagtctatt atttactaac tggccaaaca gaaatggcga taagagaaca aatgggcatt
     9121 ttgtacggag aagatgttcc atatatcaaa tggcgagaag caatcaatca atataaagaa
     9181 aaaatcgtga aagaagataa gagagtatat acgaaaaaag gagcagaaga aattttatct
     9241 tttgcaaaag aaagaggaat ccatacaatc gtggcttcgt ctaatacacg ggtaaaagta
     9301 gaaatgtatt taaaaatgga aaatctatat gccttgtttg atcaaatcat ttcaggggat
     9361 gatgtaaaaa aaggaaagcc ggaaccagag atctttttga aagcttgttc aaaaatgaat
     9421 attcctccaa gtgaagcact tgtatttgaa gactcgattg cagggatcga agcagcaagg
     9481 cgagcgggca ttttgtcttt tctcatcaaa gatcatttga tagggttgcc cgatcataat
     9541 gggaagcata agttgaaagt agatgtaagt ttatatgaat ccaataaagc agactatgaa
     9601 ttttatgacc tgcatcaagc aaaacgattt ttagaaagta aagggttgta tctctaataa
     9661 agaggactat tttctagtcc tctttattta ttgtcctgca cgactgaggg aacagatgaa
     9721 aaagaagtaa ttttttattt tgaagtatct accttatttg tagtgaacaa tttagaacaa
     9781 acacaaaacg catctactgt tatctaccag tagatgcgtt taaagagaag aacgagaatc
     9841 gtccatatga gggtttccac tccttatgca gattttttta attcttgcac tgcttgttcg
     9901 atcgtaacac ctgtcgctac atgattggta ttttctttgt caaaaacgac aaatagattc
     9961 aaatctgtat ccaaggttac tcgatattgt atatctttgt tatagaatgt catagccatt
    10021 ctcctttctt taaatacttg ctgaaaggca acgaacgcgt tgataacgaa attccattta
    10081 taagtatacc accaattgaa aggttttcat agttaagaaa ttttaaagtc aaaagttgca
    10141 gataaaagca aataaacaag aaaacaaaaa gtgaatcaat cgcggttatt caactttttc
    10201 ttcgattcga agacaataga agacaatgaa ttttatattt cattgcatta tccttgtgtt
    10261 attagaaaaa gcatgatata ttaaatgagt aatctatttt gaaacgtaat ttgagcgata
    10321 tatctacata gataaaactc cttttgccta tcaaatattt attgcaacga aaaaacgtac
    10381 attgatacgt atcaggagga atttatttat gaataacggt acagtaaaat ggttcaacgc
    10441 agacaaaggt tttggcttta tcactggtga agatggaaat gatgtatttg cacatttttc
    10501 agctatccaa ggtgacggct tcaaaacttt agaagaaggc caagcagtgt cttacgatgt
    10561 tgaagatggt caacgtggcc ctcaagcaac aaacattgtt aaagcttaat aaaaattgac
    10621 ttttaaccgt cttttcgaaa gaaaagacgg tttttttgta cgaaaaaatt
//
LOCUS       DAZAID010000095        10632 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000095 DAZAID010000000
VERSION     DAZAID010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.097"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..641
                     /locus_tag="ACG9BE_002342"
     CDS             <1..641
                     /locus_tag="ACG9BE_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033065.1"
                     /translation="EVVYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVR
                     TLWADGQVFLPERIYLLSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLS
                     LINSEQAGRQEIEIFVVSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECL
                     CAKKRVVLCDAASYQNGLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            862..2625
                     /locus_tag="ACG9BE_002343"
     CDS             862..2625
                     /locus_tag="ACG9BE_002343"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFN3033066.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            2709..3167
                     /locus_tag="ACG9BE_002344"
     CDS             2709..3167
                     /locus_tag="ACG9BE_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFN3033067.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3276..3959
                     /locus_tag="ACG9BE_002345"
     CDS             3276..3959
                     /locus_tag="ACG9BE_002345"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFN3033068.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4106..4720
                     /locus_tag="ACG9BE_002346"
     CDS             4106..4720
                     /locus_tag="ACG9BE_002346"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFN3033069.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            4736..4942
                     /locus_tag="ACG9BE_002347"
     CDS             4736..4942
                     /locus_tag="ACG9BE_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033070.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     regulatory      5167..5333
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            5481..6038
                     /locus_tag="ACG9BE_002348"
                     /pseudo
     CDS             5481..6038
                     /locus_tag="ACG9BE_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            6096..8135
                     /locus_tag="ACG9BE_002349"
     CDS             6096..8135
                     /locus_tag="ACG9BE_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFN3033071.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(8244..9395)
                     /locus_tag="ACG9BE_002350"
     CDS             complement(8244..9395)
                     /locus_tag="ACG9BE_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN3033072.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(9549..10079)
                     /locus_tag="ACG9BE_002351"
     CDS             complement(9549..10079)
                     /locus_tag="ACG9BE_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFN3033073.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(10178..10528)
                     /locus_tag="ACG9BE_002352"
     CDS             complement(10178..10528)
                     /locus_tag="ACG9BE_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFN3033074.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         3822 a         1829 c         2072 g         2909 t
ORIGIN      
        1 tcgaagttgt ttataaaaat tttgttctca atgacctatc actgaatgaa ttgattttac
       61 agattattag tatcgctgaa atcgacgatg cagaaaattt tattttcaag caatcctttt
      121 tacaatttgt ccgtacgttg tgggcagatg gacaagtatt tttaccggaa aggatctatc
      181 ttctatcaga aaaagaacaa cttctgtacg gaaaaattgt agaaatattg gaagagtgga
      241 aaaagaaaaa tcaaatccaa ctaagatgga atgaaaattt cattcgaaaa ttcgtcaaaa
      301 gtctttcttt gatcaacagt gaacaagcgg ggagacagga aatagaaatt tttgttgttt
      361 cagaaagttc agtcaaacaa tttttttatc gaacccaatt tcatttgtat gcggaagaag
      421 gcatcgctga aataaatcct attttatttc gttcattaga agaattgcct gatgaatgtc
      481 tatgtgcgaa aaaaagagtg gttttgtgcg atgctgctag ctatcaaaat ggattagaaa
      541 ctgaaaagac aaagatctat ccaatctcat taaaaggtgc aaacatccat ttgtatcata
      601 ttgctcagga acttcaccta ttaaaaaaac atatttcttg atgagacctt aattaggaga
      661 ccttctacaa agctttttaa ttgtctgtag aaggttttta tttgagtcct gattcgtgcg
      721 aagatctact tatataacaa gctttctctg aaattttttt aaaatgctat tgacttagag
      781 tcaactctaa gtagtagaat agataaagtg taacaaatct attttcatta aaagaagata
      841 gtacggagga gagaatgtat tatgagtaca gtattacatg tattggatgc aatcaatagc
      901 cccgcagatg tcaaaaaatt atctgttgag gaaatgaaac agttggcaga agagatgcgc
      961 caaaaaatct tgcaaaagga cagcgatgta ggtgggcacg ttggtccgaa tctaggtgta
     1021 gtagaattaa cgattgcgtt tcattatgta tttgattcac caaaggataa aatcgtttgg
     1081 gatgtttccc atcagtctta tggtcataag cttttaactg gtcgaaaaaa tggttttgaa
     1141 aaaggacatt accgtgaagt gagcggatat tccaaccaac atgaaagtaa acatgactat
     1201 tttacagtgg ggcacacatc tacatctatc agcttagcaa caggtttagc taaagcgaga
     1261 gatctgaaga aagaatcagg aaatgtggta gcatttatcg gtgacggctc cctaagtggt
     1321 ggcttagctt ttgaaggatt gaacaatggt ggtgttttac aatccaatct aatcgtatta
     1381 gtaaacgata atgagatgtc tattgatgag aatcatggca gtttatataa aaacttagct
     1441 gagcttcgtc aaaccaacgg taaaagcagc aataacctat ttaaagcaat gggatttgat
     1501 tatcgttacg tggataatgg aaacgatctg gaaacggtga tccgtttatt tgaagaagta
     1561 aaagatattg atcatccgat tgtacttcac gtccatacgg aaaaaggtcg cggttatcag
     1621 ccggcaatcg ataataaaat gaaatatcat tggcatgtac catttgatct gtccaccggg
     1681 gaggcaaagc aagtaacttc tggagaaaca tttaatatga tcatcatgga ttatttggat
     1741 aagaaaatgc aaacaagtac agctcctatt gccgcagtca atgcagcgat accaggcgcc
     1801 tttggactga aaacattcca agaaaaacat ccagatcatt attttgacgt ggggatcgct
     1861 gaacagcact ctatcacttt cgcttctgct ttggcttctc agggagtacg tccagtcgtc
     1921 ttccaatcaa gtactttctt gcaacgggca tatgatcaat tatcacatga tttagcgatc
     1981 aacgagaatc cagtgattat tttagtcggt ggcggaacga tctcagctag tgaccgaaca
     2041 caccaaggaa catttgacat tccttactta tcaagtatcc cgaacatcaa atatcttgct
     2101 ccagcaacaa aagaggatat gatctctatg ctagattggg cgttcgaaca aacagcgcag
     2161 cctgtagcaa tccgaattcc tacacatggt gtagaacatg gtacagctat cgcaaatgat
     2221 tatagtattc cagcctatca aacagtaaat aatggaggaa aagtagcaat tttagcttta
     2281 ggcggttttc ttgaattagg aaaagaaaca gtaaaagctt ttagaaaaaa aggaatcgaa
     2341 gcaacgttga tcaatccagg aacgatcaca gaaaatgacg tagaaacatt agataaatta
     2401 aaagaaacac acgaattagt catcacatta gaagatggta gtttgtcagg aggatttggc
     2461 gaaaatatca gtcgttatta tggtgattct tcaatgaacg tattaaattt tggtgcagag
     2521 aaagaattca cggatagcgt tcctgtaggg cagctttatg aacgttatca tttaacacca
     2581 aaacaaatcg ttgaagatag tctgcgtgtc ttgaatattc aataattcat tatgctcacc
     2641 atccagataa ttgtttatac tagtatctgg atggtttttt cattataaaa agcaaagggg
     2701 tggatgttat gtatcagcaa gtagaagaat atgtcaaaaa gttccaagaa aaatatccta
     2761 gttatcaatc agaaaaatta ggtttctttt catttgggga tacagaaaaa atggcagacg
     2821 agttgagcga actagttgta aaaaagataa agactggtac atcaagtggt tacgatctgt
     2881 ataaacaaga gaatgagcct ttaccaaaag ttggtcaatt agacgtggtc ctgaatggaa
     2941 aaaatgaacc agtttgtatc atcaaaaata taaatgtgac catcttgcct tttagtgaag
     3001 tcacgaaaga gcatgcttgg aaagaaggag aaggggatcg gacattagct tattggcgga
     3061 aagtccatgc tgagtttttt aaacatgctt ataaagaagc gttgaacata gaatttacag
     3121 aagagaagct ggtcgtatac gaagaatttg aagtaatttt cttttagtaa gaaaggtctg
     3181 ctgaaagaaa tagctcatta ttgtctcctg aatagaaaag ggttacattt ttatatgaag
     3241 ctgtattttt atataacatt actggaggag atattgtgga tacactggaa caaatcaaag
     3301 gcggagtcgt cgtttcttgt caagctttgg aaaacgagcc gctacacagt tcttttatta
     3361 tggggagaat ggctttggct gcaaaagaag gcggagcagt tggtattcga gcaaatacga
     3421 cagaagatat caatgaaatc aagcgaatcg ttgatttacc ggtaataggg attatcaaaa
     3481 gagattacga tgggtcagaa gtgtttatta ctgcgacaga aaaagaagtg gaagaacttt
     3541 tggagacgca agcagaaatc attgcactgg atgcaacttc tcgtaaacgt ccgacagaaa
     3601 aaacaactgc tgaacttgtc caaatgattc acgaacacgg tcggttagct atggcagata
     3661 tttcgacttt tgaggaagcg cttcaagcgc aagaagatgg ttttgatctt ttgtctacga
     3721 ctttagcagg ctatacagaa tattcacgaa agacgcagac acctgatttt gatttattga
     3781 atcaaatcat agaacaagtc gatatcccag taattatgga aggacatact gactctcctg
     3841 aacaagtaga gaaagcatat gaattaggtg ctttttcagt cgtagttgga tctattatca
     3901 cccgtccgca attgatcact aaaagatatg tcgaagccgc aagtaaagca aataactaaa
     3961 aaacacgcac ggcacagatt cttgacaaaa aagaaaaatt gatagatagt ttaaagcaga
     4021 ttctaagaag atgaaaaaag cattactttt cattttgtct tcaagatctg ctttttaacg
     4081 aattttatag aaagaaggcg aaaccatgtt tgaacggagt ccttcagaat tgacagaacg
     4141 tgaaaattac aagttgctga ttggttcagt catccctaga ccagtagcag tagtaacgac
     4201 taaatcagca aaagatatag tgaatatcgc acccttcagt tacttcaata tcgtcagttc
     4261 gaatcgaccc atcttatcct tggcgatcca gagaaaacaa gatgcagtaa aagacacagc
     4321 aaagaatatt cttgagacaa gagaagctgt tatccatatc cttgatgagg acaacgtaga
     4381 agaagtaaat aaaacagcag ccaatctgcc taatgaggaa agcgaactgt acctgactga
     4441 cttgacttta actgattcag taatcatttc agtaccagca ttgacagaag caaaagttcg
     4501 ttttgaaaca tccttatatg aacatgtcga gatacggaat aaaaataaag taacagctga
     4561 tttatttttg ctagaagtac agaaatatca tttaaatgaa acagtctatg atgataagac
     4621 aggaaggatc gatccaagaa aattgaatgc tgtaagccgt ttggcaggaa atgattatgc
     4681 agcaatagga gaaattttca cgattgcacg tcccgaataa aaaggagcac tagttatgtt
     4741 aaaaataagc tataaaccat cgacagattc aaaagaaatg aaaaaagaat atgaaacagt
     4801 gaatgatttt ctgcaagggc agtacttaga agttccgcca ttgcaagacc attttgtcgt
     4861 tactactgtt actttagatg gtaaggaaat agaaatgcca gatcaaacga ttagcggatt
     4921 gtttaattac tttaacaaat aaatatttat tccgaaaagt gaaaaaacat ataaaaaaac
     4981 acataaaact aaaaaattat caaagaatcc tggtgcaact gaaagaattt gcaacgggat
     5041 tctttcaact tatttgttat ttttttctaa aaaatattaa agattgttaa atttcactat
     5101 ttcatttcaa attccattgc ctaatcatta ggaaaagtct atcatgaact cgtaaataga
     5161 atatagtaat aaccttgtta ggtgaggctc ctatacaaac ataggctgtt gcccagaaac
     5221 gtcgaaagtc gccaatgggt aaaacagaaa ctgttggatt aaggcttttt ccaatagcta
     5281 agaatgtttt ctttacgttg tatagtgcta aaactcggac gatgagatga tgctaggtca
     5341 gaattcttgt tcgaaaaggt ttctgacctt ttctttatat ttttgtgact gacttatttg
     5401 atgagacaaa ctcgctcctc acctgacaag tggattgctg tgtgaggagg agatgcggtg
     5461 aaaaaagatc gattggtgga ggtcgctcgg attcacttct ggttgcacaa tacaaccaga
     5521 aaacacaaca agcaaaactg atctctatca tgagagattc atatgtcgat attccaggtt
     5581 acggaatgaa taagataaat gcagcttact cttatggggg agttgattta ttaaatcaaa
     5641 cattaaagga aaatttcaaa tttgaagctc cctattatgc aagtatcaca tttcaagatt
     5701 ttatcgattg cgttaatgaa ctgtttcctg gtggagtcaa gatcgatgca gaaaaatctc
     5761 tagatttaga tggcgtatat ataaacaaag gagagcaagt gatggatggt aatactttac
     5821 tccagtatgc tcgatttcgg gaagacaaag aaggagactt cgggaggatc agaaggcagc
     5881 agcaagtgat caaagctatc tctcagcagc tgaaagacgt aacttcaata ttcaagctac
     5941 ccaaagcggt tgggaaatta cttggtagta tccaaacgaa ccttccagaa agtgtgcttc
     6001 tggattgtgg tatggatttc ctcaaagaca tatgttagaa taaacaggta taaatagtga
     6061 aataaaggaa tgacaagcaa gagaaggagg aaaaaatgaa aagaagtgac aagcacggca
     6121 aaaatcgaac aggcgcttat attgccggcg cagtgatctt aatagcagct gcgggtggcg
     6181 gttattttta ctaccagcac tatcaagaaa cccaagcagt agaagctgga gaaaagacag
     6241 ttgagcaatt tgtccaagct ttgaacaagg gagattataa caaagctgca gaaatgacat
     6301 caaaaaaggc ggcaaataaa agtgcattat ctgaaaaaga gatcttagat caataccaga
     6361 atatatacgg tgctgctgat gtcaaaggac ttcagatatc gaatctaaaa gtagataaaa
     6421 aagacgattc tacttacagt ttttcatata aagcaaagat gaatacctca ttaggtgaat
     6481 tgaaagatct ttcttataaa ggaacattag acagaaatga tggtcaaacc acgatcaact
     6541 ggcaacccaa cttggttttt ccagaaatgg aaggaaatga caaagtaagc ctgaccacac
     6601 aagaagcagc aagagggaat attatagatc gaaatggtga accattagca acaacaggca
     6661 aactaaaaca attaggagtc gttccaagta aacttggaga tggaggcgaa aaaacagcca
     6721 atatcaaagc gattgcttcc tccttcgact taacagaaga tgctatcaat caggcgattt
     6781 cacaaagctg ggtacaaccc gattactttg tcccattgaa aatcattgat ggagcaacgc
     6841 cagaacttcc agctggagct accattcaag aagtagacgg cagatattat cctttgggtg
     6901 aagcagctgc tcagctgatt ggttacgtgg gagatatcac agcagaagat attgataaga
     6961 atccagaatt aagcagtaat ggaaaaatcg gtcgctcagg tttggagatg gcttttgata
     7021 aggatcttcg tgggacaaca ggtgagaaat taagcattac agatgcagac ggtgtagaga
     7081 aaaaagttct gatcgaacat gaagttcaaa atggcaaaga tatcaaattg acaatcgatg
     7141 cgaaggcaca aaaaacagct tttgacagtc taggaggaaa agctggatca actgttgcga
     7201 caacgccaaa aaccggtgat cttcttgcgc ttgctagctc tccaagctat gatccaaaca
     7261 aaatgacaaa cgggatctca caagaagatt acaaagctta tgaagaaaat cctgaacatc
     7321 cattcatcag ccgatttgcg acaggttatg ctcctggatc tacgtttaaa ataattacag
     7381 cagcaatcgg tctcgacaac ggcactatcg atccaaatga agtgttgacg atcaacgggc
     7441 ttaaatggca aaaagacagt tcttggggat cttttaaaac aacgcgtgtc agtagtgatg
     7501 tatcacaagt agacttaaaa actgctttga tctattccga taatatatat gcggcacaag
     7561 aaacgttgaa aatgggtgag aaaaaatttc gtacaggctt agataaattc atttttggtg
     7621 aagaccttga tttgccaatt agtatgaatc cagcacaaat ttctaatgaa gatagcttta
     7681 actcagatat cttgctagct gatactggat atggacaggg cgaacttcta attactccta
     7741 tccagcaagc agcaatgtat tctgttttta ccaacaatgg cacacttgtc tatcctaaat
     7801 tgattgcaga taaagagaca aaagataaga agaatgtcat cagcgaaaca gcagtacaaa
     7861 cgatcgtgcc agatctgaga gaagttgtgc aagatgtaaa tggtacagca cattctcttt
     7921 ctgctttagg gattccgttg gcagcgaaaa ctggtacagc ggaaatcaaa gaaaaacagg
     7981 atgtaaaggg gcaagagaac agtttcttgt ttgctttcaa tcttgataac caaggttata
     8041 tgatggtcag catgttggaa aataaagatg atgatgattc agcaaccaaa cgagcatcgg
     8101 aactattgca atacctcaac caaaattatc aataaaaaat tgctttttaa gtacagtaaa
     8161 aggactgtgg cgttatcgtc acagtccttt tttcaaataa acggttttca cgatgtctct
     8221 ttcacaactt ctttttgatt tactcacact tcataaactt tttttgtaaa atatttgaga
     8281 acaagtggag aaataagtgt acttaataaa atcacgataa ctaaaggtga atagtactgt
     8341 ggttcgatca attgactttg ctgtccaatt tgtaaaatga tcaaagccat ttctccacgc
     8401 gaaatcatac cagcaccgac catcaatgaa ctatgttgag aaaaaccggc aatctgagca
     8461 ccaaaataac ctccaagcaa tttggtcaaa atcgctacaa gcgtcaatat agaaataaag
     8521 agtagttgtt cactaaagtc agaaaaatca acttctaatc ctacactgac aaaaaatact
     8581 ggtataaata ctgcatatcc taaagcctct acattgtggt atacttcttc tttaaccttt
     8641 gtctgactga cagctatccc agcaaaaaaa gcaccaatga cagagctaag cccaacaaga
     8701 tccgctacgt aagacattcc taaacaaata actagcgata cgatgataat agaagaattt
     8761 gcgtacactt tttctgcaaa tgaaagtagg aaaggcgcaa tccatttgac taataagaaa
     8821 ataaaaacaa aataaattag ctgttcaatc aaaacaagcg gtaaagaagc attgctagaa
     8881 gcttcccccg ttagaaaaga taggctgaaa cttagaacta aaacgactaa aatgtcatct
     8941 accacagaag cacccaaaat tgttgatcct tcttttgtat tcactacgtt tagttctttt
     9001 aatacttcta ctgaaatact gacagaagtt gccgcaagaa taatcccaaa gaaaatcgct
     9061 tcatttcccg aaacttggaa tgctttgcct gtcaaccagc caaaaacaac cgggaagatg
     9121 atcccaagca aagcaacgaa cataccaggt cggaaatatt ttttcaaaag agataaatca
     9181 ctttccagac cagctaaaaa cattaataag aacacaccaa tctccgaaaa gtcatggact
     9241 agaatatcag gtttgaccca attcaatcca gcttggccta gcaaaacacc aacaagtaat
     9301 tgtccaatca ctgctgggat acctaatctc cgcgagatat gtgagccaat cgttgttgaa
     9361 atcaatatca ggcataaaat accaataaat tccattctca tcattccttt gtataaaata
     9421 tagcccgaac ttagcataac atacattatt gaaaaaatag gaaaatatac tcacaaaaaa
     9481 accaacaatc caaaagattt ttttggattg ttggttttta tacggtctat tttaataact
     9541 aaaaatgatt aacgtatagt catttttctt attctgatga aattgataat ctcctggatg
     9601 cccatgaaaa tcaaaataaa gccgaatatt tggaatagaa gcatcaaact tttgaatgga
     9661 ttgaacaata agacgaatcc gccaatcaac aacagaatac tactataaac actccaactt
     9721 ttagcttttg tattcatgct gaagaacaac tggaacaagc tgttgatgat aacagctaat
     9781 ccaagaagaa tcggcaggat agaaacgatt gcaggtgcaa aagcatagac gatcagagct
     9841 aagatcagga aaaaaagacc actgaacaga ccgatactcc aagaacctgt tttcctttta
     9901 aaacggtaag cctcaaaaag attcaacaac ccaagcaaaa caagataggc ggtgatcaga
     9961 taaccgataa aatgaaaaac agcagaaggg tttagcacaa tcgcaatccc agaaataata
    10021 taaacagcag ctcttaataa ggcgtatttt tgaatatttt gtttaatcgt ctcaaacata
    10081 taagaaaacc tcctttgact tattttaacg tgaactagtc attttaccaa attaaatgtt
    10141 acaaaatgag cgaaggagaa gaatatctct ggaaaaatca acttctgagg atcttttcca
    10201 tttttttgcc ttttgctagc tcatcgatca ttttatctaa ataacgaatt tctttcatca
    10261 agttatcctc gatgtcttct actcgtacac cacaaacaac acctgtaatc aaggaacgtg
    10321 aaggattggg gacaggggct tccgcaaaaa attgttcgaa agtcacttgt ttttgtagtt
    10381 gatattctag ttctgtttgg gaataacccg tcagccaaca aatgatttca tctacttcct
    10441 cttttatacg atcttttctc tccgcttttg ctacatacat aggataaacg cttgcaaaac
    10501 ttgtcgtaaa aatacgattt tgcgccataa tcagtcacct ttcttttttt caaattattt
    10561 actacttata gtatataaga aaaagagaag aaaaagcgat tattcaaaaa aacaaaaagg
    10621 attgtgacaa aa
//
LOCUS       DAZAID010000096        10461 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000096 DAZAID010000000
VERSION     DAZAID010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.098"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..636
                     /locus_tag="ACG9BE_002353"
     CDS             13..636
                     /locus_tag="ACG9BE_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033075.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1086..2135
                     /gene="trpX"
                     /locus_tag="ACG9BE_002354"
     CDS             1086..2135
                     /gene="trpX"
                     /locus_tag="ACG9BE_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN3033076.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2132..3013
                     /locus_tag="ACG9BE_002355"
     CDS             2132..3013
                     /locus_tag="ACG9BE_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3033077.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3016..3786
                     /locus_tag="ACG9BE_002356"
     CDS             3016..3786
                     /locus_tag="ACG9BE_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3033078.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4065..4919
                     /gene="aroE"
                     /locus_tag="ACG9BE_002357"
     CDS             4065..4919
                     /gene="aroE"
                     /locus_tag="ACG9BE_002357"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFN3033079.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5097..6260
                     /locus_tag="ACG9BE_002358"
     CDS             5097..6260
                     /locus_tag="ACG9BE_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN3033080.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6377..6787
                     /locus_tag="ACG9BE_002359"
     CDS             6377..6787
                     /locus_tag="ACG9BE_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3033081.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6961..9189
                     /gene="topB"
                     /locus_tag="ACG9BE_002360"
     CDS             6961..9189
                     /gene="topB"
                     /locus_tag="ACG9BE_002360"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN3033082.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9360..9644
                     /locus_tag="ACG9BE_002361"
     CDS             9360..9644
                     /locus_tag="ACG9BE_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033083.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9804..10040
                     /locus_tag="ACG9BE_002362"
     CDS             9804..10040
                     /locus_tag="ACG9BE_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN3033084.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10037..10435
                     /locus_tag="ACG9BE_002363"
     CDS             10037..10435
                     /locus_tag="ACG9BE_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFN3033085.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3640 a         1763 c         2218 g         2840 t
ORIGIN      
        1 gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca agaaatgaat
       61 gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg tcttgttttt
      121 ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga aaaaatccga
      181 gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt gaaacctttt
      241 gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt gatgttcagc
      301 aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc tgagcttatg
      361 gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca tgaactggaa
      421 caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg aggaaaagaa
      481 gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga gttgatccct
      541 gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt ggatagaacg
      601 ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat gaaacggtgg
      661 gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc aaaataacaa
      721 ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact taaatcaatt
      781 cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag aaaacaaaaa
      841 aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc atagatacaa
      901 ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt gactgccgca
      961 tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc cattgtcttt
     1021 ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt tgcaggagga
     1081 aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt tatttagcag
     1141 gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg aaccagacaa
     1201 aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta gatgagattt
     1261 atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa aatcttgaga
     1321 tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt caacagctag
     1381 tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa tcattagcaa
     1441 acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg ggggctggat
     1501 tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat cagccgcctg
     1561 ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa gtcggaatct
     1621 tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa gaagctgcaa
     1681 aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag attgcacaaa
     1741 cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat aataccattg
     1801 ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc gtcattccat
     1861 ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat caatatcaat
     1921 tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa aaaccggaaa
     1981 cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct caagctgatc
     2041 gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt acagatgact
     2101 cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt cacaaggcat
     2161 gctttgggca atattaggat tagggatttt catgacttat cggatcttag actttcctga
     2221 tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag ctattactca
     2281 tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga tagcaggact
     2341 cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag ggattttagt
     2401 gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc tttctttatt
     2461 gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt actttgacac
     2521 agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat tcttcttcaa
     2581 tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg ctcggtcgtt
     2641 agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg gtgtcattgc
     2701 attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata aagggattgg
     2761 cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc aagaattgac
     2821 gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc tattgatttt
     2881 agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg ctatcatctt
     2941 agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa cgggttttga
     3001 gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa agtcgtaaac
     3061 aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat cagagaaggg
     3121 gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt caatacgatc
     3181 gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct gatcacaaaa
     3241 gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc caagatggga
     3301 acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag aggtcaaaaa
     3361 cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag actatgtcaa
     3421 gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt atctggtggt
     3481 caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt actattatta
     3541 gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg attaacaaat
     3601 cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat ggaagacgcc
     3661 ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca agatatttct
     3721 gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga agaagtagtg
     3781 gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca ttgacatctt
     3841 cctttgattc atcctatact taaaacaata aacaattaaa catatttggg tgttttgtag
     3901 gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg acaagtaaac
     3961 gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc gtatcaaacg
     4021 ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt tctggacaaa
     4081 ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca ttgatgcaca
     4141 atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa gtaaatccaa
     4201 tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt gtaaatcttt
     4261 cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag gaagcgcaat
     4321 tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc tataataccg
     4381 atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa aagaaaaaaa
     4441 tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct gcgttagacg
     4501 gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa gtaaaagaaa
     4561 cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac tttgccgata
     4621 cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca acagatatcg
     4681 ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg catgagaatc
     4741 tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa gaagcaaaaa
     4801 aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga gcaatcgcgt
     4861 ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg tttgattaaa
     4921 aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa aagtaatcct
     4981 ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa tgataggctt
     5041 ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga accacaatga
     5101 gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag cgggatacgg
     5161 ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag gaagatgcta
     5221 cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac gagcaacaga
     5281 tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt ttcaatcggc
     5341 aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa atcattctat
     5401 cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt aaatatgtag
     5461 ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg caaaaagcga
     5521 cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa gatgccgggc
     5581 gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg aaactgattt
     5641 cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag atcaattcag
     5701 cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat tttggaatcg
     5761 attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc gatgccttat
     5821 tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt gtagatatta
     5881 aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt ttccgaatgg
     5941 atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac gctattttca
     6001 gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt aaagctttag
     6061 gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt tcgattgcac
     6121 gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg aattatgggc
     6181 aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa tctgctatta
     6241 cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca aggcttttgt
     6301 catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga tttaaccaag
     6361 agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc gtgaagccat
     6421 cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat acggtgaaat
     6481 gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca cgaatgcacg
     6541 gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa ttggtcctga
     6601 attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag aagaaacgaa
     6661 gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg atgctgttcc
     6721 gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc agattaacca
     6781 agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg gatcatcgtt
     6841 tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt tattgccaga
     6901 tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg aggtcagaag
     6961 atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc cgaaaccttc
     7021 caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt tgctggtgaa
     7081 gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg agcttatgaa
     7141 gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac cttccgttac
     7201 gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt gcagtcagcc
     7261 gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc gtggtcgatt
     7321 attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg gatcaattct
     7381 cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa gaactatttt
     7441 ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg aatgaatgga
     7501 agtccattat atagcttgct acttcaacga aaagggattg atggcagttt ttctcttggt
     7561 cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat caaacacttc
     7621 aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg gcagttttct
     7681 ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa ggaaatagaa
     7741 aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac gaaaaaggaa
     7801 aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa gatgaatcag
     7861 ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta cgaaagcaag
     7921 tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca tcagtatctg
     7981 gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca gacaactgag
     8041 atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca tgcaattatt
     8101 ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt acagcaagca
     8161 atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta tatctacgaa
     8221 gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg aaagatccca
     8281 ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga agctcagact
     8341 cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat ggagaaagaa
     8401 acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa aactgcggga
     8461 aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg aatagggaca
     8521 gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat cgaagtcaat
     8581 aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt agccaatgaa
     8641 ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag aaaaattggt
     8701 aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat cctacattta
     8761 ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat tagcgggaca
     8821 cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc aaaatgtaaa
     8881 aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga atgcgacttc
     8941 aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag agatttgctt
     9001 tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg gaaattcgat
     9061 gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg gatcgaaaac
     9121 agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa tgaaaacgaa
     9181 gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg agaatataaa
     9241 taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta ggctgatttt
     9301 atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta aattatctta
     9361 tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct gattacggga
     9421 cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg attttatcga
     9481 ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca agcatgctaa
     9541 agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc attatttatt
     9601 ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac gagagtttgt
     9661 gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa aagcaaaata
     9721 aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact atagaatgag
     9781 aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt agaaaatctg
     9841 gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt gtgcagacag
     9901 gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc aaagcacaag
     9961 aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa gacgcgttaa
    10021 aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa tcaacgcgaa
    10081 agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa atgcattaga
    10141 tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc ctgaaatcta
    10201 tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc ataatggcaa
    10261 taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt gtccagttgc
    10321 tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt cattggattt
    10381 cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa aatgaacgaa
    10441 ttaaaaactt tatttctcaa t
//
LOCUS       DAZAID010000097        10357 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000097 DAZAID010000000
VERSION     DAZAID010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.099"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG9BE_002364"
     CDS             6..305
                     /locus_tag="ACG9BE_002364"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3033086.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG9BE_002365"
     CDS             378..1739
                     /locus_tag="ACG9BE_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFN3033087.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG9BE_002366"
     CDS             1761..2021
                     /locus_tag="ACG9BE_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033088.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG9BE_002367"
     CDS             2035..2787
                     /locus_tag="ACG9BE_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFN3033089.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG9BE_002368"
     CDS             complement(2881..3486)
                     /locus_tag="ACG9BE_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3033090.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG9BE_002369"
     CDS             complement(3539..4513)
                     /locus_tag="ACG9BE_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFN3033091.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG9BE_002370"
     CDS             complement(4891..5610)
                     /locus_tag="ACG9BE_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3033092.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG9BE_002371"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG9BE_002371"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN3033093.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG9BE_002372"
     CDS             7161..8558
                     /locus_tag="ACG9BE_002372"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN3033094.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG9BE_002373"
     CDS             8639..9079
                     /locus_tag="ACG9BE_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN3033095.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG9BE_002374"
     CDS             9227..9553
                     /locus_tag="ACG9BE_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN3033096.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG9BE_002375"
     CDS             9554..10303
                     /locus_tag="ACG9BE_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFN3033097.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3287 a         1826 c         2146 g         3098 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtaaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactg
//
LOCUS       DAZAID010000098        10146 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000098 DAZAID010000000
VERSION     DAZAID010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.100"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..244)
                     /locus_tag="ACG9BE_002376"
     CDS             complement(<1..244)
                     /locus_tag="ACG9BE_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN3033098.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
     gene            complement(237..1829)
                     /locus_tag="ACG9BE_002377"
     CDS             complement(237..1829)
                     /locus_tag="ACG9BE_002377"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFN3033099.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(2339..3064)
                     /locus_tag="ACG9BE_002378"
     CDS             complement(2339..3064)
                     /locus_tag="ACG9BE_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033100.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(3218..3544)
                     /locus_tag="ACG9BE_002379"
     CDS             complement(3218..3544)
                     /locus_tag="ACG9BE_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033101.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(3581..3946)
                     /locus_tag="ACG9BE_002380"
     CDS             complement(3581..3946)
                     /locus_tag="ACG9BE_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFN3033102.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(3983..4198)
                     /locus_tag="ACG9BE_002381"
     CDS             complement(3983..4198)
                     /locus_tag="ACG9BE_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3033103.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            4327..4662
                     /locus_tag="ACG9BE_002382"
     CDS             4327..4662
                     /locus_tag="ACG9BE_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3033104.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(4738..6735)
                     /locus_tag="ACG9BE_002383"
     CDS             complement(4738..6735)
                     /locus_tag="ACG9BE_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033105.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(6833..7108)
                     /locus_tag="ACG9BE_002384"
     CDS             complement(6833..7108)
                     /locus_tag="ACG9BE_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3033106.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            7340..8074
                     /locus_tag="ACG9BE_002385"
     CDS             7340..8074
                     /locus_tag="ACG9BE_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN3033107.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            8113..9255
                     /locus_tag="ACG9BE_002386"
     CDS             8113..9255
                     /locus_tag="ACG9BE_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3033108.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            9521..>10146
                     /locus_tag="ACG9BE_002387"
     CDS             9521..>10146
                     /locus_tag="ACG9BE_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFN3033109.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT         3307 a         1920 c         1512 g         3407 t
ORIGIN      
        1 tgattaattc attatatttt ttttcagtaa ttttatatct tgtttcgtaa ttatcataaa
       61 tagcattttg ttgttcatag acaacaaaat tgctattaga tacaaagaat tctttcttta
      121 aggcactccc aaatggtccc ctccttactc catctttttt atcaagtaag cattcaaact
      181 tacgctgttc ccaagcttcc gtaaatcctg ggaaacgaat ttctggttgt atatcattac
      241 tcatgtttga acacctccag cgcaccttgt aaccacgctt cattttcagg tgttgtttgc
      301 agggaagaga tagcttctaa aagttctttt tctagcactt gctgttcttg tcgtatcttc
      361 ttgatgtcat ttccgacatg aaccatatca attggttctt cttcttcaaa cgtatctaca
      421 tagcgtggaa tatttaagtt atagtcattt tctttgattt catcaaagtt tgccaaatga
      481 gcgtatttct cgacatcttt tctttcagca taggtttcga ggattttttg aatgttttct
      541 tccgataatt tattctgatt ttttcctttg acaaattctc gactagcgtc aatgaataat
      601 acgtctcgag tttggcggtt ctttttcaat acaatcaccg tcgttggaat cgatgtcccg
      661 aagaacaagt tcgcaggcat accgatcaca gcataaatac tgccgtcttc tagtaatttt
      721 tgacgaatga ccccttctgc agcgccacgg aataaaactc cgtgtggcaa gacgatggcc
      781 atagtccctg tttccttcaa atgatagaaa ccatgaagaa gaaacgcaaa atctgctttg
      841 gattttggcg ctaattttcc ataacgatta aaccgagaat cgtctaagaa ggtcgtatct
      901 gctgaccaat tagccgaata aggtgggttc attactactg catcaaatgt gtaaggttca
      961 tctgttggcc aatccttatt caacgtatcc ccattgcgga gattcatttc ttcggcatct
     1021 accccatgta aaatcaagtt catttttgct agattgtatg tcgttgtatt taattcttgc
     1081 ccatgatatt taacgttatc tgggtgagtt aagtagttac gcacatttaa catcaatgaa
     1141 cctgaaccca tggttggatc aaaaacacta aagaatcgtc gttctttttg atccaacgtc
     1201 acgatttgtg ccatcatatc agaaaccata tgaggcgtgt aaaattctcc ggctttcttc
     1261 cctgcttctg aagcaaattg actgatcaga aattcataag catccccgat cacgtcccca
     1321 tcgtgaccta aaacttccac gtcattcaat ttcttaatga cttcggtaat cgtcacattt
     1381 cgttgttgct catctgttcc tagttttttt gattggagat ccacatcatc aaataaccca
     1441 ttaaattgat tgtaggtaga tgccaattgc acaaaggctt tgtttagatc attcaattgg
     1501 aacgttgctt gttttgcttg atccgctaag acgttgaaaa gatactctgg cgaaatcgca
     1561 taccccaaaa tatcaacgat tgttgcaatc agatcttctt ttgattcttc atctgaaagt
     1621 aattcgcgat acaacatcgt ttgtttgctt accgtatcat attcctctaa ggattcatct
     1681 gctaaaagaa ccacttgttc caacaaacgg tcggataaat atttatagaa aatcagtccc
     1741 aataaataat ttttatattc tgacgcatcc attttgctac ggagattatc tgccgcacta
     1801 aataattttt gatttaattc tgcacccaaa aagatacact cctatctctt atttaaagtt
     1861 gctatatacc ctcttatttt agcatactcc cttttagaaa aagcaaaaaa aaaaagaaag
     1921 aaacttattc ttgctaataa actacgcgaa aggagtctaa cgttgtttga tataacttag
     1981 actgatcgtt aacttatttt ttatatttct acatagttat ctttagaacc atttcagata
     2041 catatgaaac aattacatat gattttttca taaaaaaaca gtactgtcta gtagtataaa
     2101 atatacaaat ttcttaaaaa ttttttcact aaaatcacta cctgaaacag tctaaaacac
     2161 ttatatatca acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt
     2221 cgggctccaa atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta
     2281 taagtacggt catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt
     2341 attcaataac ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca
     2401 cagaataatc ttttttccca ttttcaagta accgaagtgg aatatcttta ccaactttct
     2461 ctgtcactgc attactgata cctgttagat tagctaataa atctgataaa gattgagcat
     2521 ctgtagaaat ctctgtatca gtatttatcg tgattccttt atcttcattt acaagtttcc
     2581 aagtaggtgg tatagctgtg tatgtttcta caaatgaatt aagtttttca ttaagtgact
     2641 tctcttttat atttccatct aaaaactctg tatatatggc ttgagctata ctaaaacaaa
     2701 gctggccttg ttctaataat tttacatcat tagttagtaa agctgtgttc acgttatcaa
     2761 tcgcttcaag aagagtatct ttggtgtccc taacagcttt aggtgtgcct tctggaattt
     2821 ccctacttga tacctcgtcc attttatctt ccataaaccc cttaaaatca tatctttctc
     2881 ccaaatccat agaagttttt gagaaagctt tcatttctga gtctatttct tcaataatat
     2941 ttaacatgtc tgatacatat tgtttaggct gattcgtttt taatctatat gtggtaaata
     3001 tctcaattcc tccaattatc aataaaattg ccaaacaaat ccataataat tttttctttt
     3061 tcatttttcc tcctatacac tgctgttata acagtgacta atgtaaagat tttcccagca
     3121 atcatcagac tattatttac agggaatcaa aaaaactcct tagtaagctt acacctttag
     3181 gagttcctgt ttgaacggta tgcataatac atttaaatca atttaacatt aacacctcag
     3241 tatatggtat ctctacatat acacggtctt tttttggata gtactcgaca tttatattga
     3301 tagcacttcc attgtctaac gtagctttag cccggtacgt gttatcacca ttatcatgag
     3361 tgatcgtcac atcatcacat gtccgattta agttatactg ttcttgtaaa atctgagtta
     3421 ccaatggtac agatgacttc tctagtccag atgaacccgt cattcttgaa aaaattatac
     3481 ctataatagc caatacaacg atacctataa tgacaaactt cttattcata tttactttat
     3541 tcaacttttt atctcctatc tccaaaatga ttttctctca tcagtaactc attgacatcc
     3601 ccaatagtac accaaagatg gctccagcta cccctgctat catcaaaatc gtacccattt
     3661 gttcatcttt ctctcgatat aagtaaccca taataacagc aacagcacca aataccacag
     3721 gaataaatgc taatgatact accatagaaa tccaaccaat aatcaaatac agcttccctg
     3781 tctcttgtcc atgtacgatt tgttccttca tttcattaat tgtctgctca gtacttttct
     3841 ttactgtgtc tgttccggtt gtctcatctg tttcttgtaa tgctccgcaa aactggcaat
     3901 acttactttc atttgttatt tctttaccac attttctaca atacatgtct tctcctccta
     3961 aaatcattca attgcttatt agctaactgt ttactttatt aaatacatca gaaaacagct
     4021 catttattgg tacacttaat atctcagaaa gcattttcat ttgatagggt ttaggaatcg
     4081 ttctccctgt ttgccacgaa cttacacatt gctgtgtaac attcattttt ttggctaact
     4141 cagtttggga aagtcctttt tcttttctgt atcggctgag attacttact tttttcatga
     4201 ttctcccctc ctgttttatt atatacaaca atatgatgta tttcaactca taaaatatat
     4261 aaatacttca aatgtcatta caaatacaac aacttgttgt actctactga gaaggggggt
     4321 gtattaatga aacagaagca atatgcaaag gtcattagag aaaagcgaaa agcactcggg
     4381 tggacacaaa aagaattagc taaaaaaatc ttctctacac aacaggcagt agcaagatgg
     4441 gaaaattctg taacagaacc taacctcgat agcttaacag ctctttctag agccctagga
     4501 acccctgtaa gccattttct agacaatgtt gacatagatt atgaggaaga gtttttggct
     4561 ctctatcgct ctcttagtac tgaagatgca attcgaacaa ttgactatat gaagctatta
     4621 aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt gaaaaataaa taacccaccg
     4681 aactttaacg gtgggtgtat tgagatgaaa taagattaag agctatggat aacctgtcta
     4741 gtttaatata attgggtctc ttctattcct tactctccgt ccatcatctc ttgttacact
     4801 tgtaaattga taaataggaa ctaattcaac tcctgataat atcaattctt ttattacttc
     4861 atctatccct gcccctttcg ctagatatac taaaaaacta ctgcttgctt tcacattgta
     4921 tagtttctta atatccttag aagctcgttc aagagtgaat ccttgtttaa caaagtagcc
     4981 tctgttttct attaaagcat ttttcacgct gttatccctg acatttcgta ggcgttcaca
     5041 aacagtgtta ataagttctt tttctgtcat tagatatgtc ccttgccacg ataagccctg
     5101 taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc ttgctaagtt ctgatcggga
     5161 attttgagga aatactcttt tagcctcttt ttcgttaaaa catatcttaa cccattcata
     5221 agaaaaatat tgatatgtaa aatgaaattt ttccagaagt ggaaggatat cttcattcaa
     5281 tttttgaatc acagaagttg cattaaaaac gtctaatcga tagacattgc ttctcttatg
     5341 tttaaagttt cctaacgctt tctcttcctt taacctattt aggtattcga gcatcttcaa
     5401 aggaacatct tcatcatcta atttttttat caatcctata aaagtcataa gtgatagtaa
     5461 cttattacat ttatctatgt cataagtttt accatagtaa gaactcattt ttttagctaa
     5521 ttgttccccc gacaaaatac aagtcacact ttccttacta tcatcaaaaa aattaacatc
     5581 agaaagcaag acatcataga cacgtcgcat ttcctctaga taattcctta ttgtcctgaa
     5641 aagtttgtct ttcattgatt caagagtgtc aaaaagtact tttgattcct ctttcaattt
     5701 tcgaaaatgt gtaggataca agtctaggtt ctctaatagc caattaaatg tttctacccg
     5761 actgtctttc tttaaaatta gcatcacaac tccaataaaa ttttcactga tatcacaatt
     5821 actggaatga caataaaaaa tggaatgccc agttttactg gtaaaaatac tagcagaggg
     5881 attatgatcg ttgtgaaaca gacatcttaa gtttttactt gacttaagta atttattcat
     5941 atcaatggtt aacaatcttt cataaatctc attaaaagac atgtcggagg aatcaaacag
     6001 gcagtcttta aaccactgtt tcatttcttc tttgtcgtca ttttttatca acttaacaaa
     6061 atcggtttca ttgactagtt catactctct ccctgaatca ctttgacaat ttttagaaca
     6121 tgtcatggta aaatcaatcg gaagctcagt aatatctaac atgttgtctt cattaattat
     6181 tatcactgca tctgacttat atcctccaaa aaacattcta ttcgaagcat ttacattgtc
     6241 atcacacccg gggatgttct ttttcaagta ttcgtaaaca gcaaccattt taccatagtc
     6301 agtaattgct tcttttaata gaaagacaat cttgtatttt tctagatttt cagagtaccg
     6361 aatacttttt tgtatcgcaa acgcagaatt atgaactaaa ttgatagata aaaccttttc
     6421 tatgcttaag tatctactat catctttact caaatctact accttcccct tctcatcttt
     6481 aacagtgtta tcgacatcca gaataattac tctggtttcc acaaaactct ccttgtcatt
     6541 gcctttattc tttgtcttgc ttggaataaa tgattgtgat gaatttagta tctctacaaa
     6601 ctcatctctt gtaacttctc tatagttatc taaacaccta tctcttacat cagttttttc
     6661 tttttttgta actggcttct ctaataattt ttctttatct acataaattg gaatattaat
     6721 cattgatgtt tccatttgtg tttcctcctt ataaaattct gttaaagact aaaacataca
     6781 gataataaaa acaaactctt tattactata tgttttagtc ttatttgttt actcaacctt
     6841 tataccgttc catgaaacta tcaatatcag attttttaaa tctaatgacc gtatcaattt
     6901 ttataacagg taacccgtat tgagaaacat attttctaat cgttcctact gatacaccta
     6961 gatattcact tgcctgctga tagtccatat actctagagt gactccagaa ataatgtctt
     7021 ctagcatagg ctttagcact gtaattatct ctttcacttg ttgttcaact tgtatagaaa
     7081 caatgctatc aatgatttgt agagacatat ttccccctcc tttcgtgatt gcttgaacaa
     7141 attttctaag aattatcttc gtattttttg ctcaaaataa aagtattttg ttattaaccg
     7201 attaatcaaa caaaaaatga ttaccagttc atcgacatac acataatacc tcgataaaaa
     7261 aggggtgtca atagttttgt aaaatttatt tacaacaccc cttttttgag gtatactaat
     7321 tttaggaggt gtggtcaata tgataaaacg tccaccaata aattatttag aacgaaaaaa
     7381 gatactcgga acaaaaataa aagctataag aaagagtaaa aaattaacac aaccagcatt
     7441 tggtcttatg attaacaacg gtcaattaat tgacaaaaag actatctacg aatgggagaa
     7501 gggaacctat ttaccaatac cagagcgatt atcccgaatt gctgatttgg gaaatatgag
     7561 tatagaggag ttagtttgtg gcaatgtaga agaatatatt ttgggaatta ttctttatag
     7621 agatagcatt gtactagatg gtataacatt tccagataaa aacctgttcc agcatcttcg
     7681 tcaacagttc ccaccagtac actctaattt agatacatgg ctggatagat attccaaatt
     7741 agaaccagag atgcaagaat ttattgctaa caagacttgt aataaagtta aaaatgaaaa
     7801 aattagtctt ttcaatatct taaaaataga agaattattc atcaatgcta ttgtagaaga
     7861 atttgacaat aacattcttt tcctcactag cagtatagaa gaactactag aaagaatggt
     7921 agatgaatgg ctacctattc aattgaaaga catgtcttat cccgaagaag ctgtgagaga
     7981 aattactgat aatataaaca agctagaaca aacgatttca tcaattggga aaaaatacac
     8041 taagaaaaaa atgaagggag gtgataccat ctagtggtaa gtactccttg taaactaaga
     8101 ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga aaaacggtca agtggcgtgg
     8161 atgtataaag gacacattgc caccgatcca cgaactggtg aaaaaataaa tactacgaga
     8221 agaggtttta gtagcaaaca agaagctcaa aaagattatg acgagtacag acatcaaata
     8281 ttatacggtg ttaaaaagaa agcacctgat atgacatttg aagacctgta taatgaatgg
     8341 ataactcatc agcgtaccag tgttaaagct tcaacaatcg caatctcagt aagatacgca
     8401 aataatcaaa tacttccagc atttggtaag cttaaactat cgaatatctc tgtcccatat
     8461 tgtcaaaaag tggtagatga atggcacagt aaatatgaaa gttacgatta tatgagaaaa
     8521 cagactgcac aaattttacg ctacggggtt gcaatgcaat atattgacaa taatcctatg
     8581 gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa atcgtaaatt ttactcaaaa
     8641 gaggagctta ataatttact ggatgctttt aaagattttg gtaacatgaa acaatacgcc
     8701 ttcttcagat tattggcata cacaggtatg agaaagagtg aagtactcgc tttacagtgg
     8761 aaagacatag acacgtttaa taaagaacta catgtcaata aaactcttgc tgtagatgaa
     8821 tttggtaaag ttataattca atctccgaaa acaagagctt ctagacgagt tatctcttta
     8881 gatactgaga ctctttctat cctcaacaac tggaaattac agcaaaaaga agagtattta
     8941 aaacttggat acaatactag ctctaaagag caacatgttt ttacaacagt taaaaacact
     9001 ctatacattc caaacactgt taatgattgg ttaagatata ttttgaaaaa atataacttg
     9061 cctcgtatta cacctcatgg gtttagacat acacatgcta gtttattatt agaagcagga
     9121 gaatctgtca aggtagttca acaacgatta gggcatgaaa atagtaaagt gacattagac
     9181 atctatgcac atattacaaa taatgcacct aagaaaacag gtcaagactt tgctgatatg
     9241 atggctcatc agtaaaagag ctggtcaaaa tctggtcatt tctagtatac aggcaaaaca
     9301 aaaaagctag aacccttata tatcaagggt tctagctcct ataacaggtt tacccaacac
     9361 tgccttccaa tgactctttt ggttctataa tattttgtgt tggtggaaaa tcgaaagcga
     9421 ttttctacta acactttttt cttcgtttca actcaaaaag agactcactt ttggtaaaat
     9481 ttagtcgacc aaaacgtcac cctaccaaag gagtctctca atggataatc atactagaaa
     9541 attactcgga ttaacagata aacatttatt ttttgatgaa gaatggctga ttgaaaaaga
     9601 atataaaggc gttcaagcgc agtttattaa agggagattg gatgactcac cttcccattg
     9661 tgaacactgt ggatcgattc gtattatccg caacggttcc tatacgacac gcactcagat
     9721 gttgaaagtc aaagaaaagt tgactatttt agaattaaaa cgtacccgtt ttctctgcca
     9781 tgattgtgga cggacctttt ctgcgaaaac ggacttagtt gatgaacatc atcaactaac
     9841 aaaggaactg aagcaggcga ttctcatgga actgtacgaa aatcaatcac gtaaactaat
     9901 cgctaagaag tacttcgtct cggatgggac ggtgacacgg atcttgcgtg aagcaacgaa
     9961 gcattaccag ccacgtatga acttccttcc gacggtgttg tgtatggatg agttcaaatc
    10021 aatgaagtct gtttctggtt ccatgagttt tatttgtgtt gatgggacaa ccaatcagct
    10081 atttacgatc cttgaagatc gcagacttta caaactaact caatacttcc tgcgctttcc
    10141 tagaaa
//
LOCUS       DAZAID010000099        10140 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000099 DAZAID010000000
VERSION     DAZAID010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.101"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..2704
                     /locus_tag="ACG9BE_002388"
                     /pseudo
     CDS             8..2704
                     /locus_tag="ACG9BE_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            2769..3269
                     /locus_tag="ACG9BE_002389"
     CDS             2769..3269
                     /locus_tag="ACG9BE_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3033110.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            3281..3775
                     /locus_tag="ACG9BE_002390"
     CDS             3281..3775
                     /locus_tag="ACG9BE_002390"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3033111.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            3796..4605
                     /locus_tag="ACG9BE_002391"
     CDS             3796..4605
                     /locus_tag="ACG9BE_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN3033112.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            4602..5423
                     /locus_tag="ACG9BE_002392"
     CDS             4602..5423
                     /locus_tag="ACG9BE_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3033113.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            5474..7573
                     /locus_tag="ACG9BE_002393"
     CDS             5474..7573
                     /locus_tag="ACG9BE_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFN3033114.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            7570..8688
                     /locus_tag="ACG9BE_002394"
     CDS             7570..8688
                     /locus_tag="ACG9BE_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFN3033115.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            8727..9605
                     /locus_tag="ACG9BE_002395"
     CDS             8727..9605
                     /locus_tag="ACG9BE_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN3033116.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            9751..9948
                     /locus_tag="ACG9BE_002396"
     CDS             9751..9948
                     /locus_tag="ACG9BE_002396"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFN3033117.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            9932..>10140
                     /locus_tag="ACG9BE_002397"
     CDS             9932..>10140
                     /locus_tag="ACG9BE_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033118.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSI"
BASE COUNT         3109 a         1667 c         2272 g         3092 t
ORIGIN      
        1 gtaaaaattg gaatacaaat atagaataga catattgcag ccgatagttt attatgcttt
       61 tagacacatt aaactggagg aaaaaatgaa aaagagcgct aaagatcgag tttttgatga
      121 gctgatacga ctaacaggga atatgtcttc tgtcactgcc gagaaaattt cgaagaatct
      181 aggtatctct cgtcagaatg ccagccatta tttgacgaga ttagtggaag ataaaaaggt
      241 cgagaaatta ccgggaaagc ctgttttgtg gaagccttta gatgaatatg cggttgtaga
      301 taatacggag caaataaacg aagcctttca ttctgtagtt ggtcatgacg gaagtctgag
      361 agaggtcatt caaaaatgta tagccgctgt caaatatccg cctaacggac tcagtgtatt
      421 aataaatgga gccacgggtg ttggaaaaag ttttttagcc acaaaaattt ttgaatacgc
      481 tatccatgag caaattattg aaaaagatgc accatttgct atcttaaact gtgccgatta
      541 tgcggacaat cccgaactgc tttcggcaac actgtttgga tacaagaagg gggcctttac
      601 gggggctgaa aaggataccg atggtttatt ggcaacggcc aacaacggct atctttttct
      661 cgatgaggtc catcgcctat cgaaagaaaa tcaagaaaag ctctttctat ttattgatac
      721 tggaaactat cgtccagtcg gtgaaaatgt aaattggcat tctgctaagg ttcgttttat
      781 tttcgccacg acggaaaaag gtgaaaatta tttattagac acctttgatc ggcgtattca
      841 aattagtgtt atgttgccaa cttttgaaga tcgccccatt cgagaacgat tagagctgat
      901 tcaattattt tttcaaaatg aagcggatgt tttacaaaaa gatattattg tttcaagaga
      961 agccttgtct ttaatgttat ctcatccctt tagtgggaat attggtaagc tgaagaatat
     1021 cattaaaatc agttgtgcgg atgtgtatag tcgaacacaa gaagaaccat taacgattgg
     1081 gaaaaatgag atcttgattc agttaaatat gctggaatcc gaagtagaaa gcttgccgat
     1141 tgctagttta ttgataaaga aaaatcaaaa gataaaaacg gatcgaatca aaccgtttga
     1201 ttatgattcg attttggaaa aaattactca gctgataaat gaaggtaatt tcaatgaaaa
     1261 tttttcttcg ataaagaatg aagtacagca tttggtgcat cacattgata aggattcttt
     1321 tttatcaatg agtagaaaat tgatgattca gatgtttaga aaggtctggg tatccgtttt
     1381 aggaaaaacg tacggattga cgacgagtgg acctgttgca gaaattatta gcagatccta
     1441 tgtcatttcc ccaagctata agacagactt aaccgggata aatcgtacat tagctgcaaa
     1501 tctacctcgg acgacttact tatgtaatca gttcttggag catttaccac cgctctcaga
     1561 aaaggacgag aactttctaa gactgcttat tgtcgtgact ttatctgatt atgtagatga
     1621 atccatcgag ctgaagggcc tgttgcttgc tcacggagat agtacggcta gcagcattca
     1681 agcagtagtg aatcaattat gcggcaatta tgtttttgaa gcgttggata tgccagtaga
     1741 gagtaatgtg tcagagattg tcgaatcgac aaggaatttc gtgaaacggc aagtgaatac
     1801 agatagtcta gtgctgatcg tagatatggg ctctttaaat caaatttaca cacagatcaa
     1861 gaacgattta aaaggggagt tacttcttct gaataatttg acgacttcca ttgcacttga
     1921 tattggcttg aagatgacga tgaatgcgcc atttaaatag attgcggagg atgcagcaga
     1981 caagtattca atcaatgttc aatactttga aggcttttct caaagtagca acattatcat
     2041 ttcctgtatg tcgggtttgg ggatttcgga caaattgaaa gatgtttttc gtcatcattt
     2101 atcggatgaa tccatcgaag ttttcacgaa ggactacaga gaactacgtg aattaatcga
     2161 ccaaaatgat gaaacttatt ttgacaagac aaaattggtc atcacgactt cggatcttcc
     2221 acgttccttt tcaattccta atattaatgt ttatgacatc ttagacggtg atggagcagc
     2281 caatttggag catattttga gtcctgaaat gaagccggca gaatttaacc gcttgattca
     2341 ggagctagta aaattctttt cgttggaagg aattgcggac cgtttgaact ttctgaaccc
     2401 aacgatcgtg atgagtgaag tggaaacagt catttctaaa tatgaaaatt actatcattt
     2461 cactttaaat ggccgtgtaa aactaaatct ctatatgcat acggcattga tgatggaacg
     2521 tttattcttg gctcggacag aaaaatctga agaaccgaat tatgatttga aaccagatga
     2581 agatgaattt tattatgttt ctagaagtat ttttcaatca atggagatga aatataattt
     2641 taaagtcaat gcttatgagc tttcgctgct ctatgaatta ttaggaccgt atattcaaaa
     2701 ataaagtgtc taaaaaagtg tctaatctgt tttttttagg ttagacactt tttttaatgt
     2761 gaattaacgt gattaaacca atgtttttaa aagttggcac aggaattgct tatataaaag
     2821 tgaacagaaa gaaaggaatt aaccatatga agaaaattct aatagcgact cacggtcact
     2881 tagcttcagg ctttcttagc agtattcaat tactgacggg gaaggttcaa gagattacgg
     2941 tgattaatgc ttatgtggat gagtgtgatt ttgaacagga gttgaatgta tttgtaaacc
     3001 aggtgaacga gcaggatcag atttttgttt ttaccgattt atttggcgga agtgtcaatc
     3061 agaagattac caaaaccttt ttggagaaag agattgctgt aactattttg tctggcttta
     3121 atctgccaat tctgctcgaa atagtattgg caaccaagga attaacaaag gaggatattc
     3181 aggaactggt aaataaatgc caacaggaat taaagatgac cattcttgat gaggtacatg
     3241 tagtaaatga agaggaagat tttttttagg aggaagaaaa atgattaaag cagtgcgaat
     3301 tgatgaacga ttaattcatg gacaagtagc gatgacttgg acaaagagtc taaatttaac
     3361 cgggttagta gtagcaagcg atgaggcggc taccaacgat attcaaaaga tgacattgaa
     3421 gatggctgca ccatcgaatg tcaaagtcat tattaaaaca gttgatgacg cgattcattt
     3481 gttaaaagat ccgcgggcga aggacatgcg cttgatggtt ttagtcccta atgtcaaaga
     3541 tgcggtaaaa gttgcgaaag ctttcccaac ggaaatcgaa ttaatgaacg tggggaatgc
     3601 tgggaaaatg acaccgccat cggaagataa gaaaacctta tcaaaggaag tgatgttaac
     3661 gacgtcagaa attcaagact taaaagaatt agttgaactt tatccagata ccgtttttca
     3721 gcctacaccg gcgatggaaa aacgttcagc tatctctatt ttgagtgatt tttagaaaaa
     3781 ggaaggagaa aaattatgta tgatgcattt gtagtcgctt tggccgtttt tattggagtt
     3841 gctggacatg aaatgttcgg gatgtccatg ctgagtcgtc cgattgttgt tgctccgtta
     3901 gcaggagcgt tgatggggga tttgcagacg gggttgattg tcggggcgtc actagaagcc
     3961 atctttatgg gtgtcgtcaa tatcggaatc tctagtacgg cggagccttc tttggctgct
     4021 gggctggcta cagcgttcgc aatcaaaatg ggaggaaacg tggatgcgat tattcccatt
     4081 gtctttccac tagcagtatt aggattacag ttaatgaata tgattttttc ttttgtatgt
     4141 ggaccgatgg ctgctaaatt tttcaaattt gcaaaatctg gtgatcaaaa aggaatcatc
     4201 aatctgcact tctttatgtg gtttgtccac tatgcgcttt atgctcttat tccattcttt
     4261 gccgttttat ttggagcaga tgttgtcaaa agcgtcttgg ataatattcc agaggtcatc
     4321 atgaacggat taacagtcgc tggaaatctg ctgccggcag ttgggatggc gatgctgtta
     4381 cggatgcttt ggagtaaaaa tattgctgtg tggtttttct ttggggccgt tgttatggcg
     4441 tatctcgagt taccattgat tgcaattgcc gcagttggaa cgattattgc aattgttata
     4501 gcccaaagag atctacaact aaaaaatata tctgttcatg cagttcaaag cgatgctgct
     4561 gtaaatgcaa cgcttgacca agaagaggag gatttctttc aatgaaatta gctgatggaa
     4621 tttctaaaga tgagaaaaaa ttggttcgga agatgttttg gcgctcgatg actatgtatg
     4681 cttcggtgaa tccggtgacg atgggcggcg gtggtttcgc ctattctatg atgcccttca
     4741 ttaatcattt ctacaaaagt gaagaagatc gagctgcggc gctggaacgt catacacagt
     4801 atttttcgac gaccattcct tgcgcgtcct ttgtcatggg gattgcggca tcgatggaga
     4861 aggaaaattc tgaaaaagct ggttttgacg tggaaagtat caacgcgatc aaaacgagtt
     4921 tgatgggacc attggctgga attggcgatt cattgttctg gggagtttgg cgtgtcgtta
     4981 ccgcgggact agcgatcaac ttagccaatg ccggaaatat tttggcacca attatctttt
     5041 tagttatgtt caatatacct aattacttga tgcgatacta tggcggcgtt ttaggctatt
     5101 cattaggctc taaatatatt gaaaagctgt atgcgaacgg gttaattgaa attctgacaa
     5161 aagctgctag tatcttgggt ctgatcatgg taggtgcaat gacgatcagt atggtcaaat
     5221 tcaataccgt tttgagcttt tcaatgaagg gcgaagaact aatgaatgtc caagaaatcc
     5281 tcgatagtat ttttaaagga attgtaccgt tattggtaac attgagctgt ttcaaattac
     5341 tagataaaaa agtcggcgtc atcaaaatta tttttggaat ggtcgtcttg ggaattttat
     5401 tgtcattact tggaatcgca tagtaggaag ctgcagctac taggctgcag tttttttagc
     5461 aaggagggaa aagatgacta ttaatgaagt aatttattca cccaaacacc cattttttaa
     5521 tgctgagtta aacgacggaa atgtgactac ttcaaacgaa tacagctact atcggaatat
     5581 tcctgatgta aagggattga ccttcgatgt aagtaatcaa aagacattag caaaccttga
     5641 tgtttgcggg acgattcaaa cgttgacctt ttatcgagaa aatttactaa ctgaggaaaa
     5701 accaggtgtt tgggtcaata aacagctctc tcaaaccaat tcgttatcct taaaggttga
     5761 agtaaacggg attgtctacc atttgtcaga atcggatcat cgtattgaag tcgatgtgat
     5821 tcaagactgt ttgcctagat atattcatca ttattcgacg tttaaggtca ttgtagtatc
     5881 ctttgctccc atactggcta ataagcgttt aagtatgctt gttcaacagg tttacattgt
     5941 gaatgaaaca cacgaaagcc ttcaagtttc agtgaaagat gtgccttcgt atcaagagaa
     6001 gtattcggat cagcaaaatg tgttgatttc tcaaaaagga aataaggaca cgatcgcaca
     6061 acaggaagtg gcaagcttcg ccattgcttt tgtggaccca aatgcttttg aagaagtaat
     6121 gacttttcag gaatctgata tcgaaaaatg gctgaaggaa acattgaact attttgctca
     6181 gctatttggg cagctgtcaa tgccaaacga tagagttgtc catctctatc gtcgtgcact
     6241 ttatcaaagc ttttctagtt tcggtatgaa tcgacaggga caggtcgtcg gcagcaattg
     6301 gggcagctat cctgctacta atcgaatttg gaataaggat atgtattacg catcattgcc
     6361 attcaccatg tttgaaccgg agttatgtca aaaaacggtt ctatggtttg atcaatatgg
     6421 cgttaagttt cctggttcaa aatttacagg aggtatcaat cattctctga gtaattcgtt
     6481 atccagtgcg ttactggcta gtctttattt tgaacatact ggggatacaa aattctttga
     6541 gcggtgtccg caggttctac tgaatgcttc aagaattatt gaggacatat tagctcaacg
     6601 tcggtcaggg gaaccaatgc tttttccatc tgtttggtta tcagacgcct ttgcacttgg
     6661 aaagtatcat accggcagca atctttgtct ttggaaggcc tgtattggac ttgcagaaat
     6721 ttttgaagcc ctagaacaac tgagtcttag caagcgatac aaaaacattg cctgtaaact
     6781 aaaggaatca atttgtaaac aaatgactat cgatggtcct tttggtgagc aatttttgga
     6841 gggaatcggt gatgaggaga aaacaaccta ttcagtgaag cactatcaaa aaccgattct
     6901 tgagcaaggg ttaatctttt taagtgatgt gattgtggat ggaaaaatta atttattgat
     6961 gcatgatgga gaagaatcag acactacgtt gatgcctttt tatcaatttt tagataataa
     7021 agatcattta tatcagaaca ccatggcctt ttctgctagt tctttcaatc caacctatag
     7081 cgaggaaatc aaaggcatca cttggggatt agaatccggc gccacttttc ctggctttat
     7141 cactgtttta atgagcgagc tgcacaaccc tcaagctttc gtaacccgat tagaagaact
     7201 aatttgtttg gcagatttag acggttcgtg gtggtggtgg ccgtatcgct tagaggctca
     7261 acaaggagaa gtcgtgcgag actttggctg cggcaaatgc ggctgggcat cgggtctttt
     7321 tgtcagccta atgatcagtc aatatttcgg attgaaattc aaaaaaggag tattgcaaat
     7381 tgatcctgtt gataatttaa cgttttcttg gaagaatttg aagttcggtc aagccttcat
     7441 ttcgattgaa tgtacgcaat ccgagttgag tatttcaaat ttgtcagaaa agccattgaa
     7501 aataagtgat acgaaaaaaa ttcttcacgt agaaaaggga aaaacgattc acattcaaag
     7561 gagaaaaaga tgagtatact aaaaaatatt acccaattta aaaaaatcca gagtcgagca
     7621 atcactcccg aaaatccaac aggcgagaaa ggtaaagctg cgatgttgcc aagcgagcta
     7681 ggaccaagcc gcaaaggtcg tggcgcaatt ccgctgactt ctggtgaaga aactgtgatt
     7741 gcggatatta ctggaacggg aataattcgt catatgtgga tgacgattcg tgatcgaaca
     7801 gaatttggca gttttgtttt acgtgattta atcttaagaa tttattggga tgggtcggat
     7861 agccctgctg ttgaaagtcc tttaggggat ttcttttgta atgggtttgg agagcgttgc
     7921 gaggtgaact cgttgccgat agtggttaat cctacagggg gctttaattc ctatttcgaa
     7981 atgcctttcc aaacatccgc taaaattacg atcgagaatt tacatccgaa ggatatgtcc
     8041 tcattttttt atacgataaa ttacgctttg gtggacgaat tggaagaaaa tagcctatat
     8101 tttcacgcaa attggcatcg ggaacgcatt accaaattac aggaggatta taagttaatt
     8161 gatcgattaa agggtcaagg ctattacgtg ggtacttacc ttgcgcttac ctgcctagaa
     8221 cgttattggt ggggcgaagg cgagtttaaa ttctacattg atggggatag tgagtatccg
     8281 accgtaacct caacaggttc agaagattat tttggcgggg cgtgggcctt tcatgagtac
     8341 gatgagttcg gacgtccaca tgcgaaaaac tattcaacag cctttttagg gtatccttac
     8401 caatcaaata gagatgccac aagagatcgt tttgaaacgg gaaaattgaa tgccgttcat
     8461 gcctttggtg acgacgcaat gccacgacat ggtctttatc gttggcatct tcaagacccc
     8521 atctgtttta ccgaagattt atcggtcact ttacaacaaa tcggcaacga tgatgttcgc
     8581 ttatttgagc ggcaagacga tgttgctagt gtcgcttact ggtattcaac agaagcttca
     8641 ggaaatacta aacgtcattt cactgatcga gaacgcttgc cgcgttaatg aagtgttttt
     8701 cacatattcg aagggaggaa cctatgatga tatatggggc aattgaagct ggagggacta
     8761 aatttgtctg tgcagtcggt actgaggaat taacgatttt ggaaagagtc agttttccca
     8821 caacaactcc agttgaaaca atgaagcagg tcattgattt ttttaaacct tttaaggaac
     8881 aattggcagg catcggggtc ggctgctttg gtccaattga tattcaatgc aaatcaacga
     8941 gtttcggtca tatcacatca acaccaaaac ttgcttggca aaatttcaat tttgtaggaa
     9001 cgctaaagca atatttcaat attccgattg aatggacaac agatgtcaat gcggcttgct
     9061 atggtgaata tgttgctggt tctggcaaag ggttagcagg tgttgcatac ttcactgtgg
     9121 gaacaggtat cggaggtggt gccttgttca atgggacttt cgtggaaggg ttcagtcatc
     9181 cggaaatggg gcatatgttg atcaagcgac atccaaagga caatttttca ggaaactgtc
     9241 ccttccacca agattgttta gaggggatgg cagcaggacc agcgattgaa aaacggttgg
     9301 gtgtaaaagg ccagaattta ttggctgacg attcgttttg gcagattgaa gccttttacc
     9361 ttgcccaatg tgcatacaat acaacactga tgttttcgcc agatagaatt atttttggcg
     9421 gtggtgtaat gaaacaagaa cacatgaaga aaaaagttca ggataaattt gttgagctta
     9481 taaatggata tgttgaaata ccaccaattg atagctacat tattacgccc gaattaggtg
     9541 ataatgccgg aataatcggc ggattagcat tggccagaaa agcggtaaga aataagcaac
     9601 cataattaat gaatgtttca ttgatactca tgtcaaaagt atttaaccga gtaatcaaat
     9661 gatcaaatga agttggataa agcgaaaatg aattcaaata aaaaggctct aagtcaaatc
     9721 gtgttgtttt cattaaatca ctgaaaggaa atgcttctgc gtttcctttt ttctttttgt
     9781 tgtgcgctgt ataccttgag gatattcact ggtggcgtac acttaaatag taatatattg
     9841 tgttacttta tgtctgtggt aatagttata gttgcttacc aatgtatgtt catttttcga
     9901 aatgttgact tcataatcct tggcttaatg tttgaatttt tttggtgata gattttttca
     9961 gccgtataaa ataaatcgta attctattat agagagttgt gattataaaa agatagcaag
    10021 aaaaaaaaag aaagtttcta tacattttaa atattttatt cctatttcta ttgctaaaaa
    10081 agtagaaatt ctatatttac ctttaactct cgcttctctt gcaaatgtag ttagcatttt
//
LOCUS       DAZAID010000100         9945 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000100 DAZAID010000000
VERSION     DAZAID010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.102"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACG9BE_002398"
     CDS             complement(<1..613)
                     /locus_tag="ACG9BE_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFN3033119.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            814..2028
                     /locus_tag="ACG9BE_002399"
                     /pseudo
     CDS             814..2028
                     /locus_tag="ACG9BE_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2207..2542)
                     /locus_tag="ACG9BE_002400"
     CDS             complement(2207..2542)
                     /locus_tag="ACG9BE_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN3033120.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(2526..2789)
                     /locus_tag="ACG9BE_002401"
     CDS             complement(2526..2789)
                     /locus_tag="ACG9BE_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFN3033121.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(3249..4052)
                     /locus_tag="ACG9BE_002402"
     CDS             complement(3249..4052)
                     /locus_tag="ACG9BE_002402"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN3033122.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(4030..5154)
                     /locus_tag="ACG9BE_002403"
     CDS             complement(4030..5154)
                     /locus_tag="ACG9BE_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3033123.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(5170..5985)
                     /locus_tag="ACG9BE_002404"
     CDS             complement(5170..5985)
                     /locus_tag="ACG9BE_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3033124.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5975..6847)
                     /locus_tag="ACG9BE_002405"
     CDS             complement(5975..6847)
                     /locus_tag="ACG9BE_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN3033125.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6932..8221)
                     /locus_tag="ACG9BE_002406"
     CDS             complement(6932..8221)
                     /locus_tag="ACG9BE_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3033126.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(8365..8877)
                     /locus_tag="ACG9BE_002407"
     CDS             complement(8365..8877)
                     /locus_tag="ACG9BE_002407"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033127.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8944..9087)
                     /locus_tag="ACG9BE_002408"
     CDS             complement(8944..9087)
                     /locus_tag="ACG9BE_002408"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFN3033128.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            complement(9570..>9945)
                     /locus_tag="ACG9BE_002409"
     CDS             complement(9570..>9945)
                     /locus_tag="ACG9BE_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3033129.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         3054 a         2110 c         1610 g         3171 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccac caacacaaaa tattatagaa gcaaaatcaa ttctacctta gatgttctta
      781 tatcactcta tcagtcctat ttgacaaaga gccaagaaaa agacgttggt cgtcgatgct
      841 gttctctcgc ctactcctcg tgcttgtaga agttgtggtt ctactgtggt agatggaaac
      901 gggaaagcaa ttatagtgaa aaatgggaag aaggaaacga ttgtccgttt tgaacaatac
      961 aatcatatgc ctttggttac gcgcctaaaa aagcaacgct atacctgtaa aaactgtcgt
     1021 acccattgga cggctcaaag ttattttgtc cgactcagcc attcaatcgc agatcatgtt
     1081 cgatataaaa ttgcttcttt actgactgaa aaagtatctt tgtcttttat tgcgaaaagt
     1141 tgtcaggtat ctttgactac cgttactcgt acattgaaag agtttaaaag ctatttacca
     1201 aagccttcta agaagattct ccccagagta ttgatggttg atgaatttcg ttcgcacgct
     1261 tccatagaag ataaaatgag ctttatttgc gcagatggtg aaacaggaaa cttaattgct
     1321 gtcttgccta cgcgtaaatt acctcgatta acaagctatt tcttaggctg caccaatcca
     1381 gaagaagtag aattcttagt cacagacatg aacgccgctt acttccagct taccaaacgt
     1441 gttctgccaa atgcgaaagt agtgattgat cggtttcata ttgtcaaaca catgaatcag
     1501 gcactcaatg agttgcgtat ccgtgaaatg aatgagcttc gtaaagcagg acagaaaagt
     1561 caggcagaaa aactgaaaaa gaactggcgc tttctgctaa aaaatcgtgc aagcatcaac
     1621 cattatgagt acaaaacatg gaaaagtttc cgagcaccaa aatacccatt tctcactgaa
     1681 gcaatgatga ttgatcgatt gcttgagttt tctgcgcctc taaaggaggc gtatcccttt
     1741 tttcatgaat tagtcgaagc ctttcgagac aaagaccctg acttattttt ctccttattg
     1801 gcagaacttc ccgaaacgtt agatgacggc tttcgggaga agcttcaaaa ccttctgacc
     1861 tatgaagaag gtatcaccag cgcaatgatc tatccttatt ccaatggaaa aattgaagcg
     1921 aagaataccc acataaagac aatgaaacga gtatcctacg gatttaaatc attcgagaac
     1981 atgagaatta gaatcttttt gatcaatcaa ttaatcaatg taagataaca aaaaatctga
     2041 accagaatgt ggttcattct gacccggatt tgattttgca caactcatca gtccttattg
     2101 acaaagagcc aaaaatctga gccagaatga ggttcattct gacccagatt tgattttgta
     2161 caacccatca gtccttattg acaaagagcc cttcttttta gtattattat aattcttcca
     2221 tgcgttcttc cagtcgctgt aaatcgcttg gacttaattc cccaattttt ttccatttga
     2281 cctttttgtt gtttttgggt aattcgttta gagttcctac gtcaatccaa gattcttttt
     2341 ttagtcctgc ctgtttccaa tcccttattt caacgtattt cagctttatt ttatcggatt
     2401 tagtatgata ttttgaagta attgacagaa acgaaattgt gtccagtcca ttttttagaa
     2461 tatagacagg ccggcgttta ccgccctctg cagctgtgaa agcaacgtag acggttgcta
     2521 tatcattagt attcatcttc attgaaccat tcttcaattt tttcgtcagt gtctagtttt
     2581 tggacaggca atttttccgc taatctttga atattatgga ttcgtttact tttttccgac
     2641 aattcaatag aaaatgggat ttttccttgc tcaacaattt gcttataaaa aacggtgacg
     2701 gctgttttgg ggctcatgcc tagttcatca ataatgcttt ctgcttcatt tttcaatttt
     2761 tcgtccatag aaatttgtaa cttagccatt ttctccacct ctttctgaca aacacattat
     2821 atcacggaaa gagttcaaat ggaattaata caactacttt tgaactcctt gaaatgcagt
     2881 gtatttatct aaatataagg aaaggatata gtgctaaatg gccttgatag atagaaaagt
     2941 ttcaaaatcc gccacagcga taattgaagc gaacactcaa cgacacacag aacaggaaaa
     3001 gactcctaaa agaaccaaat taactaggac gtgtctgaaa actccacaaa gatgtggtat
     3061 gctctggtaa aaaacgaatg gcacaaccac gaagaccgat atggttcttg gaaaactttc
     3121 tccacccttt ttgtaattgg agggatagtg gtttattgat agcgattttc caagttcttc
     3181 aagtggaacc agacattgag caccacggct aaaaagtgaa tgaagtcttt atttttaatc
     3241 aataattttt aatgattacc agtaattggt tttttcatgc gccctatttc tatttgatgt
     3301 ggagttaaat aatgctgata agtaaaaatt tttccgtcaa ttaaactgcc actagtgaat
     3361 cctgttctat tggtaaaaag agaatagtct gggtattcat ctacaccaaa tgataaagat
     3421 tcagccataa actgtttttt accagcaaat tcagcactgc taccttgatg gatatgtcct
     3481 acaaaaatac ctaaaatatc actttcggca atcaattcag caaaaccttc cggaacttca
     3541 gttacaatag atggatcttc ccacgacaat ggatgatgaa ccaataaaat cgttcccttt
     3601 tcagcaggtg tacccaacca ctcttgtaat tgaatcatct gtcctatcga tatttttcct
     3661 tcgtgctggg tatcttgagc agaatccaag gaaataattc gtaacccatt ttgattcgta
     3721 caatggatat actctcccgt ttcattgctt tcaacactca ttccttcaaa gaaaacactg
     3781 cgacgatcat gattcccacg acaaaaatag taaggtgtgg ttgggaaata ttgatccaac
     3841 attttccgta agacttgata atcagaaatc cccccttcat gtactaaatc acctgttaat
     3901 agaataaaat cgtacactgt taacgagagt tcttgtaaca atttttccaa tgccaactgt
     3961 ggatttttaa ttgttggaaa tagttcagca gtaaacaagt tagtttgata gttttctcta
     4021 aaatggatat cagagagatg caaaaagcgc atggctattc cctctttctt caataaaata
     4081 atcaggatat cctaaatttt cttgcgtctg ataatcaaaa aaatgcattc tttcaggttc
     4141 tatgcggaga ttgactgcat caccaatctg ccaagtaggt ttttcattaa ggacaatcat
     4201 ttctatacca tcgactttaa cggtgtacgc ataactttga cctaatagct cacgatactt
     4261 cacttgaccg tgtaaatcgc ttgtttcttt tgaaaaggtt acgtgttctg gtcttatgcc
     4321 taaatacaat gagccactaa cctgaagaag ttgtttttgc caagctggat tcaatgttaa
     4381 aaattgactg gctaccttta atgttccttg atcataagta actggcataa tattcatgcc
     4441 tggcgaacca ataaaatttg cagtaaaagt atttttaggt ttcgtataaa catttttcgg
     4501 cgtatcaatc atctgaattc taccttcatt caataaaaca atccggtcag ctaacgtcat
     4561 cgcttcaatt tgatcatgag tgacgtagac aaaagtttgt ttataacgtt gatgaatttt
     4621 taccaattct tttcgtgcat ccaatcgtaa acgcacatcc aaatttgata acggttcatc
     4681 taataaaaaa taattactcc gtttaaccac cgcacgagcc aatgctacac gctgccgttg
     4741 ccctcctgac aaatctttag gaagtcgatc ttggtatggc gttaaaccta acatcttcaa
     4801 agcttcttcc aaacggtgct gaatgtcagc tgtcttaatt ttattcgccc ttaatgcaaa
     4861 aataatattc tcctttacag tcatgtgagg atataaagca taattttgaa aaaccattgc
     4921 gatctctcgt tctccacaag gtagcttatt tgctagttgt gtacctaaaa aaagctctcc
     4981 actcgtaatt ttttccaaac cagcaatcat tctcagcaaa gtagattttc ctgaaccaga
     5041 tggtcccagt aacacaattc gttctccagc ggccacagct aaatcaattt cttttaaaat
     5101 ggtatgttct ccatattttt ttccgatatt tcttaattca agcgtttctc ccatgatttg
     5161 tcctccaatt tatttaatac ctgcagaaac aaaagtcccg ataatatatt tttgaaaaat
     5221 tgcatacaag actaacggta caaacattgt aattaaacta atcgccattg ctacggtaaa
     5281 atttgtccca ccttcagaac taatataggt ctgcatcgcc aacggcaaag tataattatc
     5341 tactgtccgt aaaattaata ccggccaaac atactcattc caagaggtaa taaaaaacca
     5401 aataccggta gaagtaatcg catgtctagt aagaggaaaa acaattttac tcatgacggt
     5461 tgtttgtttg atgttatcca atgccgccac atctagaagg ctcactggaa tttcccgcat
     5521 tgtttgcgtc aataaaaaaa tgcccatcgc atcagcaaat tgtggtatta tcacacccca
     5581 agtagtatca attaaattaa gttttgcaat caacaaataa ttaggaatca tcgcaacagt
     5641 aaaaggaatg aacatcgtac caatgatgat aaagtaccag attttcttcc ctttgattcg
     5701 gcaataagta aaactatatg ccgcaaaaaa agctacgcct aacttaacaa ccgttataat
     5761 tgtagcaata aaaaaagtat ttctcgtaat tttgataatt ggaagatttt cgaacaaata
     5821 gcaaaaattt tccaaggtaa acggctgtgg tatcaaacta gtaatcgtct gataggcatc
     5881 tttcaatggc ttaaaagaat tggccataat aaacaataat ggatagataa taatgattat
     5941 gatgaacata aataagagat gtggcaacca gcgtttaatt ttcatagtaa accccctttt
     6001 ccacaaattt aaatattacc cctaagaaaa cagcaaatat taataaagtt aaaactgaaa
     6061 ttgctgaaga ttctccgact ttatacattt caaaagcctt ttgataagca taatagatca
     6121 cgtttgcact accattattc ggtccaccac gagtgatgat cttaatcgga gtaaacacat
     6181 actgcagacc ttgaacaatg gtcatcacca ataaatatag tcctgtcgaa ctactcatag
     6241 gcaacacaat tgtaaaaaaa atgcgccaga tgggtacacg atccaactta gctgcttcga
     6301 tcatctcttg atcaattccc acaattgctg caaaaatgac aatgaaatta taaccaaaaa
     6361 ctttccaagc agtaatacct gcaatcacag caattaccca accttgtaaa ttcatccaca
     6421 taggcagtct taaaccaaaa tttcccaaca agacagcaat tggtcccgat accggattta
     6481 atatccatgt aaaaaccatc cccgccacta ccaaagaaat cacagctgga ataaaaaata
     6541 atggtttata caaattctgc cctttttcta taactattcc taataaaaag gatgtaatat
     6601 atgggaacac acagctcagc aagacaaata gaacggtata ggacaaagta ttcttcaaca
     6661 ctttatgaaa gactggatca gtcagcacct ttacatagtt ggcaaagcca acaaattctt
     6721 tagtgggggc caccatattc cagtcaaaaa aactcaaata gatcgtataa atcaatggat
     6781 aaaaaacaaa cacgcccaac aaaataattg ctggcgccag atacaaataa cctacatagc
     6841 gcttcatttc cgacagcctc ctcatcctta tgtcaaaagc agacgacctt gtttttcctt
     6901 tcttacaggc gctgtcgtct gcttgctttg cttatttgag taactcatta atttcctttt
     6961 gagtagcttt catcgtttca ttgacggaat tacctgataa aattttatcc cgcatatcca
     7021 acaatttttg ttcagcttct aatccagcat ctccaggaaa tgaactccaa ctgaccatct
     7081 tatccatctg atcaattgct ggtatcatca tctgattttc agctaagaaa gcttttaatc
     7141 cattttgggc gtctgcaaca tctttacgtg gtggtaaata accagtacct tctgtccatt
     7201 tagctaaaga ttcaacacta taaagatatt ttaagaaatc ccaagttgct tcctttttct
     7261 tatccgtttg tgatgtaaca gctaacattg cgccaccagc aggtagttta acttttttcc
     7321 cttcccaact tggtgactca actgctttag cagtaaaagt actatttttt tgaatgttgt
     7381 ccctttgagc aatagtggta tagaccatcg cgacgttacc atcaataaag ctttgcacac
     7441 cttgttccca cgtagtgtgt aatgccgtct ggtcttttac catcctttga tataattcat
     7501 aagcagcgat accatcttta gaagcaaagg tagctttgcc atcttttatt acacttgccc
     7561 cattactttc taaaattgct tgttgtgccc aattatcagc aggttcttga atatacacgc
     7621 catactttcc agttttttct tttattatct tagaaaaagt ttgaacttct tcccaagttt
     7681 ttggaccatt ttcatccaag cccgctgctt ttaatatatc ttgatttaaa tataaaaccg
     7741 gattactaat ggagtaagga atacccactt gtttaccatc tttttcagac agtgccatga
     7801 tattgtctaa aaaatgatct ttaaaataat cagcattatc cggatccact tcttctacta
     7861 aagattgagg attagtatat ttgaaatttt ctgcaaaata atctttaaaa gcccatccca
     7921 cttgtactac gtccggtatt tctttggagg caacagaagc ttgtaaattt tgcatcaagc
     7981 ctttatacat atcaggatta tattttgcta caaccttcac tttgtcttga gattgattgt
     8041 atttatctac taattcattc actgtttttc cacctagggt atccgcatta acatgccaat
     8101 actctatcgt tacacgaccc tccgattttt ttgttgtctt ggtattccca ctaccacaag
     8161 ctgccaaaga gatgacactt aaaacagaca caacccctat aacaagattt ttctttaaca
     8221 taacattaac ttcctcctaa tttctttatg gccatttttc taatcaagaa atattatgaa
     8281 ctaccaatgt aaatttctag ttaattttct gtaaataatt cgaaatttgt gcatttcggg
     8341 gtacagggtc actggacata tccatcaagc taagaataaa gcccccatat ggtcatgtat
     8401 cccatctcca aatctcttaa ctcctctgtt ttgattataa aatagtttat tctgtgctcc
     8461 tttcttccat aaataggccc atgcacaacg tgaggttata ttataaacac cacagcgcgc
     8521 aaggtactca atcgtttcta gttcattgac tgtccataca gaaaatgtca atcctagctg
     8581 ctgccccact tcacgggcca acttagttaa ccatccttca tgaatattga cgatttgaac
     8641 gctttgatag gctaatttct gtaaggtatc gatcaaatct gcttcggtaa tatccccaag
     8701 ttttttccct tcgaactgat tttcaatatt ataaaatata tgctttcccc aaatcgcagc
     8761 ccaagataat tgtacatagc cactaagaac aatttgtggc cagatttgat aggtttgtgc
     8821 cagcgaaaaa acggccattt ccaatccctc ttcttttaaa tcacagttaa tggtcatggt
     8881 tgccttctca ttggccactg cttgaaacac ctgctcaagg gttaccgaat ctgggacaac
     8941 ttcttacctg tcatgggcta gtattagcgt accatctgtt tgctgacgca catctacttc
     9001 aaaacaaagc tggcgatcca aacaaaagcg aagaaatgcc cagctattct cctccgtatt
     9061 gtcactacca gagtgtgcag tgatcatggt cattttgacc cctcctttaa ttccgtgatt
     9121 ttctcccaca cgtccactat accgatgaag cgttaatttc ttgttaattt cttgtaaatt
     9181 ccctgcgttt tttcagtaaa ctagttgtaa atcttgcatg ctccttccta cgaactcatg
     9241 aatcgtcgat tttgcccttc tagctagttt caccaattaa gtagttctac attccccgct
     9301 tttaacctac tacacctttt tttgttttca gctattcctt cttcttgctg aaagtccttg
     9361 aggttctttc ttacaggata ccatcaaggt gttcaatggc ttattcccat tgaatgaccg
     9421 agacttacta tcagatttct gctcgttgaa ccaagtcttt gatacaggat ttcttccgtc
     9481 atcttctcac aaataccacc ttgcgtttct ctagcgattg gtcgatgtgc accctcccag
     9541 tggactgtca ctaccccttt ttgctatgcc tagcccacaa aaaaagctgt cctcacagac
     9601 agccagactg tagacaaaag ctgttccaaa ctcgtaactc gagcttggaa cagcttttta
     9661 tctacttcgt ttcgaatttt tgaacaaaaa aataaaggct ccttgccttt ccatagccag
     9721 gtggctaatt tctttaaatt cattgcagca aacactagca tcgtatgcgt cgcgactttt
     9781 tccaagcctc gatacttggt ccaacgcatc ccatgctttt cttttgcatc cgcaaatatt
     9841 cgttcaatcg tttgtttccg ttttttgtaa attgatttat tcagatctgt taggcgaaga
     9901 tgttccactt catccatcag atctccccaa acatgtctcg tgatg
//
LOCUS       DAZAID010000101         9503 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000101 DAZAID010000000
VERSION     DAZAID010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.104"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..1720
                     /locus_tag="ACG9BE_002410"
     CDS             74..1720
                     /locus_tag="ACG9BE_002410"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFN3033130.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            1711..2793
                     /gene="uxuA"
                     /locus_tag="ACG9BE_002411"
     CDS             1711..2793
                     /gene="uxuA"
                     /locus_tag="ACG9BE_002411"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN3033131.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(2901..4454)
                     /locus_tag="ACG9BE_002412"
                     /pseudo
     CDS             complement(2901..4454)
                     /locus_tag="ACG9BE_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            4602..5743
                     /locus_tag="ACG9BE_002413"
                     /pseudo
     CDS             4602..5743
                     /locus_tag="ACG9BE_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(5869..6948)
                     /locus_tag="ACG9BE_002414"
     CDS             complement(5869..6948)
                     /locus_tag="ACG9BE_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN3033132.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(7051..7491)
                     /locus_tag="ACG9BE_002415"
     CDS             complement(7051..7491)
                     /locus_tag="ACG9BE_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN3033133.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(7548..7958)
                     /locus_tag="ACG9BE_002416"
     CDS             complement(7548..7958)
                     /locus_tag="ACG9BE_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN3033134.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(8138..8572)
                     /locus_tag="ACG9BE_002417"
     CDS             complement(8138..8572)
                     /locus_tag="ACG9BE_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3033135.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(8672..>9503)
                     /locus_tag="ACG9BE_002418"
     CDS             complement(8672..>9503)
                     /locus_tag="ACG9BE_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN3033136.1"
                     /translation="FIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGILLEAAL
                     GFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITSDRLLSV
                     IFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVSLNLFIF
                     GIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIGRGLTLL
                     EAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGFTYHPIE
                     "
BASE COUNT         3314 a         1707 c         1708 g         2774 t
ORIGIN      
        1 ttgacatagc gatttttatt gcataaaatt cacttgaata caaaaactaa tatgttagtt
       61 taggagggga aaaatgattg atttaaacaa ctggaggaac tttatccctg aattatctca
      121 aaaagaagta gaaatacctc attttgaccg tgcatcatta agagaaaatg gacaaaaaaa
      181 tcctgtctgg atccatttcg gtggcggaaa tctttatcga ggatttcatg cagaaatcgc
      241 tcaacgttta gcggatctag gcgaattgtc cactggtgtc attgtttgtg aaacattcga
      301 tgaagaaata attgaaaggg cttaccaaga ttttgatgat ttgattcttg aagtggtcat
      361 gccaagcagt ggagagttcg tcaaacgtat attagctgcg acagctgaca gtctgtattg
      421 tcatccagat tgttctgaac actttgataa gatgattcga cactttgaaa atcctagttt
      481 acaattagtt acttttgcta ttactgaaaa aggttatcga ttgaaagata caaataataa
      541 atatatagga ccgattgcag tcgatataaa aaatggtcca aagaagccat cacatacaat
      601 gagtattctt gctgtgctac tttataaacg ttttttatct ggagcttggc cgattgcact
      661 tgtctctaca gataattttt cacaaaacgg tagtcaatta cgtacttctt tgctaacgat
      721 cgcaaaagaa tggcaaaagg aaggttatgt acaaaataca tttatccaat acttatcgaa
      781 tgaagaaatt gttgcgtttc catggacgat gattgatcgt atcaccccga atccttcctt
      841 gtcagttagc aaaatgttgg caaaagaggg cttttcaaat atgtcgattt tccaaaccgc
      901 aaaaggaaat aatattgctc catttgtcaa tacagaggac atccattatt taatcatgga
      961 agatcatttt cccaatggac gtccgaaatt cgaaaaagct ggggtattct tgactgatcg
     1021 tgcaacggta gataaagcag atacgatgaa ggtgacgact tgccttaatc ctttgcatac
     1081 gtctttagct attatgggtt gtttactggg gtataggagc atagctgagg agatgaaaga
     1141 tccagattta tcgctgctag tttctgaaat cgggtacaat gaaggcctcc cagttgtgga
     1201 gtctccaggt atcattgatc caaagatctt tctggaagaa gtaattactg tgcgcttttc
     1261 taatccaaat attcctaata tgcctcagcg aatcgccacg gatacctctc aaaaggtggc
     1321 gattcgttat ggtgagacga ttaaaaaata tatggaaata gaagttaaag acccgcagac
     1381 acttgtattc ataccattga ctctcgctgc ttggcttcga taccttattg gcattgatga
     1441 tcaaggaaaa ccattcatcc cgagtccaga cccactattg acagaccttc agcataagct
     1501 ttccgatatt caactgggta cgcaagatac atcacatatt catgagaaaa tggctcctat
     1561 tttgtcaaat aaagaaattt ttggtgtaga tctttaccaa gcagagcttg gcagaaaaat
     1621 agaagaaatg ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa cattacagga
     1681 ttacttacaa gaacatggag gaaatttatc atggaaatga cttttagatg gtacggatca
     1741 caggaagaaa agattacctt agatgaaatc agacagattc ctgcggtcaa agggattgta
     1801 ggcacattga tggatatacc agtaggagag gtatggccta gggagaggat caaagaacta
     1861 aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt caatgtgcat
     1921 gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta taagcagaca
     1981 atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat gcctgtattt
     2041 gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac gttagctttt
     2101 atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcga acattccagc
     2161 aatgaatttg gattgccggg atgggaacct gagcgcttag ctcctatcaa atccttgctt
     2221 gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa attttgccta ttttctccaa
     2281 tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc agatgatccg
     2341 ccatactcga tttttggatt agctcgaatc gtaaagaatc gagaagattt agattggttg
     2401 tgtcgcatag tagacagccc ttataacgga atcactcttt gtacagggag tattgcagaa
     2461 gatccagaaa atgatgtata tgggatttta gcagaatttt gccaaagaga tcgaatccca
     2521 tttgcacatg ttcgaaatat taaattttta gctaatcgag atttctatga ggcaccacat
     2581 aaatccgaat acggttcatt agacatgtac cgcatactaa aaacaatgta tgacaacgga
     2641 tttaacggat acatccgtcc tgatcatgga agaatgatct ggaatgagac aggaagacca
     2701 ggatacggct tatatgatcg agcattaggt gctgtttatc taaatggatt atgggaagca
     2761 atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa aactgtaatt ttattatgta
     2821 aataagaagg atgttcaaat tcttgataca taaatccgga ggttgttaat aagcattcgg
     2881 atttatgtat cttatttggt ctaggaagta acttgatttt cttttttcga ttgatccatg
     2941 agtgcttctt cttttaattc aaagatatat tctgagatcc tcataatcac ttttttccag
     3001 tcttctggtg tgatgattcc ttttaaaata tagatttttt tgatatgaga aggcaattgt
     3061 ttcaaagggc agtcgctgat aacgatgttg cagttctcca taaaatcatt ttgtattttg
     3121 atcgattcat ttgaaaaaat ttgttctatc ttaacgatca gatctttact gatataaaaa
     3181 tcacgcgtat agttcacacc aatcacaatt cgcggctgtt ttttgtagcg atcatacaaa
     3241 tgtactaggt cttcaaccat ccattgcaca ttttcctttt ctaaaataac tggtaacaag
     3301 tgttgatttt gttctctgaa atccatataa aattgttcaa tggcggtttg gacttctttc
     3361 aatacgctat gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg ttgattgaag
     3421 ttaagaaatc cattataaag tttatttcgt gcgaatgcca atgagtaaag aatcttttct
     3481 ttttcaggta taaacaaatc aaatacagta caaagggact ccaccatttc tcttgaataa
     3541 agaacatgag gaacgtttgc tttttttaaa agtttgtatt gctccaagct tgtttcttcc
     3601 gattcgattt tcgtgataga aagtcgagct aaaatattca ctaagatgcg ttgttcgcga
     3661 caaattggca gattaggtcg caaaatatct acagagagat caataaaaaa gtcaatatat
     3721 tcatactctg tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa attctcttta
     3781 catattttta atagaaaata taatttatct atcataccgc tatttagtcg gtcaagtact
     3841 tcttttttaa aaaaagtatt catcataaaa gccactactt gcggatcttc atcataaaat
     3901 tctgtagcag tacttgaaaa gatgttccaa taaatatcta atgctgcata ttgaatttgt
     3961 acctcttttc cagaaaaaga ctttctgcca ttattagaaa aggagataga caaaccgtac
     4021 tatgctaaaa atgcattaat ctttttcatt ttcgtataca aataagaggc actatagttt
     4081 gtttgctgac tgaaactgat aatcgaattt gtctgacagc tgaacaaaga agtcaagatc
     4141 tgataactga ctgattcgtt gcagtatctt ccaaatagac gataatagca gtaagccgga
     4201 tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg cagttctgtt
     4261 agattgagac gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa atttgattca
     4321 gacatatgga aatgatctgc taaatattgt gtagtaataa agaaacgatg ttcagatagg
     4381 aaaagaaaaa tatctaattt tgtattgctt tttttcttca agataaacgt tttaatcgct
     4441 tcttgagttt gcatgaaaga ggctcctttt tacttaaatt gttaaaactt gaaaatatta
     4501 ctgttttttt cataaattta gacaaaaaaa cagtaaacat ataggtagaa tctctactat
     4561 cttatttctt gcatacaaaa aaatcaagga gtgagagagt tatgagatat tcaaccatct
     4621 taatatcaac atttgcagcc ttaagcttat cgcttactat aaatcagaca gtccacacag
     4681 aggagacaga aacagccaaa tggacagaaa ccaagtgggc caacgtcaat cgaattacct
     4741 ttcaggatga attttcatcc ttcaattcag gagtcgcaaa attaagaaaa ggacaaacag
     4801 cgaccatcaa agcaaaaatt gactattcgg gagacttagc acctatacta caatcagctg
     4861 ttacatttga gaatgttgat cccactttgt cgattggcta tgacgagaac actttgacta
     4921 tccaacacaa gcaagcgata tttaccttca ccgttacatt aaatcaggac ttgttaaaac
     4981 cagcgctttt tactgtcaag gtatcagatg gactaccgaa cgatcatcat ttaacgccgt
     5041 atagtcagcg gcagacaatt gagcaagatt cagccatcga ctcaggagga gatcttgttg
     5101 aagaaccaac agataaacca gaaaatgaaa ataagccaga agtaccgcct acagaaaatc
     5161 cagacggaga acagaaaccg gaaatagaac ctggagaaga acccgataca gaaactcaac
     5221 cagagccaga caataaatca aaacctgaaa taacccctgg agaaaaacca gatgtaactc
     5281 cagaaccaga tacggattct ggaaaccaaa cagtaccgga aacaaatcca gacacagata
     5341 atgaaactga aaatccagaa aaaccagagg tagatccaga agaaaaacca gatgtgactc
     5401 cagaaccaga tacggacgca agagaccaag gaataccaga aaaaataaac aaaaaaacaa
     5461 ttcaagagga tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta aaaataaatg
     5521 aagagcagct gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct gaaaccttaa
     5581 aaagtagcaa ggatacaacc tttgctgtcc tgaaacgaaa aataaacagc taccaaaatc
     5641 cggagagtca caaaacaaag ttatactgtg gagcggaatc attctattaa gtatagcaac
     5701 aatgctttct gcaaaaagat ttaaacaaaa cagatctctc taacatttga ataatgtctg
     5761 tgaacgcatt tttcaaataa aaagaaatat ccagaaaatg accccaaaat accagattga
     5821 aatctgatat ttggggtcgt tcatacttta tctttcttca tatttatttt aagggatatc
     5881 tgtttctttt ggaagatggt tcgggatctt tttcactttc aatacatcta aaaagaaggt
     5941 aaagatcgga atgccgacaa tcaagcccca aacgccaaag aaccgttcgc taacaagtaa
     6001 gaccacaaat gtataaaaaa tcggcagttc tgttttgctg gacatcaact tcggattcag
     6061 cacataagac tcaattagat ggacaatcaa aatcgtaatc aggatataaa caacatctcg
     6121 gatgcctcct tgggaataag ctatgaaaga tagaggaata caagaaatga taacacctgc
     6181 tactgggatc agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct gatgaaagcc
     6241 aaatacagcc aaggcgattg tagtgagcaa cgtatttaaa agagcgacaa caaattgagc
     6301 ttctaatacc aaaccaaatg tattgacaaa cttatcggca aaatagtaaa tatcttgaaa
     6361 gaaccaagaa aattcacttt ttaaaaacaa tcttgagaat acaattgttt ttttcttttc
     6421 aatcataaaa aagaaactta agatgaatga catagcaaac gccactgcca tttttcctat
     6481 atcctgtaaa tagccaagcg caatcgaagc gccattttga agctgttcaa aaaaatcgtt
     6541 tcgctcaata taagtatgga tatattgtaa aacaggatct gcatcttttg gctgatgctc
     6601 gtaaaaatta ataacggact ggatcatgtc aaatgtttga tgaataagca tcgggatata
     6661 ctttgtgaat gccagataga taaggtaaac gatcagtgta taaatcatga tagctaaaac
     6721 acctgtaggg attcggacaa atcgctgaat gatttgaaca agatgaatca tcaaataagt
     6781 aaagataaat gtgagcaaaa tggttgtaat catacttcta actaagaaaa gaacaaaaac
     6841 gacaaataat aaaacagtga gacggcgtat tttttcattc gccaaaaaac gctgataaac
     6901 ggtcaaatcg acaacctcca aataacaaaa aataaacacg ttattcatta tatgatggaa
     6961 tatgaaaata agaaagagca aaaagaaaaa ccgctaaatg acgttatctt atgagttggt
     7021 tttcctcata aaatcgttca tctagcagtt tcagcccttt ttctggtctt gatgaaaatt
     7081 atctagcatt tttcgggtca gatgttgcag tgtatccaat tcttcctgtt caagtggaag
     7141 ataagacttg atcttttgtg gaataccttc tgcttgtttt tgcaacgcag caccttgagc
     7201 agtcagcgta acatttacga ttctttcatc acccgtttca cgctgacgat taatataatt
     7261 tttttctgct aactttttta gtaagggagt cagcgtacca gaatccagac acaaatcatt
     7321 tcctagctct ttcactgaag tctttttctt ttcccataaa actaacatca caagatactg
     7381 ggtataggta agattcagcg gcttgaggag gggagtgtag tgtctaatga tttctttaga
     7441 ggcggcatat aaaggaaaac atacttgatt aactaaatat agatcttcca tttttccacc
     7501 tccgctgttt tactatagtg tatcatagcg gatttacaca aaaaagtcta ttcactaact
     7561 gctttcagag taacttctat atttccagct gtagcttttg aatacggaca cactttatgc
     7621 gctgcctcta ataattcttg cgtttcttcc agagaaagac cttcgataga tccttcaatc
     7681 tctacaccta agacaaagct cgtttcactt tctgaataaa gggaaacttg tgcactgaca
     7741 gtagaagccc cagagatatt tttttgctgt ttgatcaatt ctaatgcact gttaaaacat
     7801 gcactgaaac cagctgcaaa caattgttca gggttggttg cattttcgac gcgtttacca
     7861 ggagctgcaa tattcaattc gaatgtacga tcgggattat gcacttctcc agagcgtccg
     7921 cctgtgttga tcattttcgt agaatacatt tttttcataa agttaccacc tttaattata
     7981 ttttatacaa ttgaatttta cacaatataa ttgtggaaag caaagaaatg gattggcata
     8041 agataaataa gagaaagcac atgaaacaga cgcgctttct cgaatagcaa acatatacaa
     8101 aaaatatcag agacaaaaaa taatctcatc ataaacatta catatatttc tcaaaaagtt
     8161 gttttctttc ttcaaaaatt acttcacgat ggacctgatg aagcatccat aaataaccaa
     8221 aaggatctct gaacattgag ttagtcacac caaagtccat catttctgtc atttcttgaa
     8281 tctctacaca gccatttcgt aatgctttat tatgtgtgtc ctcgatatca gagacgatga
     8341 tattgaacca gatcgtattt ggatgttcag cagtaggagc agtcaaacca agagacgtgt
     8401 tttcatccag catatggaat ctcgtaccat acaaggtaaa aatgacttca ttattgcctc
     8461 tagggtaatc tgttgcctca atcagttgta tttcatcaaa gattgtttga tacagttcta
     8521 gtgctttcaa actatcattt aatacaaaat cgatttccgt tcctttatgc ataaagatct
     8581 cctctccttt tcagtcgaac agttactaga ttcttttatt ataccagcta aagaaaacgc
     8641 attcaaaaaa atagcggatt tacttaccaa ctcattcgat tggatgataa gtaaatccgc
     8701 gtcccattac ttctgctacg ctgctgacaa tgacaaaggc acttggatcg atttcttcta
     8761 tcaatggttt cattcgggaa aattcatgat cagtcacaac gatcaataaa acctcttttg
     8821 actttcgatt atacccgcct tttgcctcta gcagggtaag gccacgtcca atctcagcac
     8881 ttaagcgaag gcggatgtct tccaaataat gttcactcat gaccataaca gatttctttc
     8941 gattgatacc tgtttcgata taagtcatga caattgaagt gattgcaata gataaaatag
     9001 cataaagaaa ctcttctatt ccaaaaataa acagattaag agaaacaacg aatgcgtctg
     9061 taaaaaataa gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg aggggaggga
     9121 tcgtcgtacc gccgctagaa gagttgtttt tatataaaat cgcaactcct aaagcaaaaa
     9181 tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt aatcattgtt tctggaacta
     9241 ttgcaatgat taaaggaaaa accaagcttc cgaaaagtac tttaaaaaag ggttttttac
     9301 ctaaaaatag aagagctagt accaacataa cgatattcaa cactaaaata actatagctc
     9361 ggtcaaagcc taaagcagct tccagtaata ttcctagacc gctgacacct ccagcagcaa
     9421 tatgatgagg ccctaaaaac atattgatag cgactcctaa tatgaagagg gcaaaaataa
     9481 tcttaatgta tgtccatata aat
//
LOCUS       DAZAID010000102         9459 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000102 DAZAID010000000
VERSION     DAZAID010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.105"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..1108
                     /locus_tag="ACG9BE_002419"
     CDS             134..1108
                     /locus_tag="ACG9BE_002419"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN3033137.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1090..1551
                     /locus_tag="ACG9BE_002420"
     CDS             1090..1551
                     /locus_tag="ACG9BE_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3033138.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1601..2065
                     /locus_tag="ACG9BE_002421"
     CDS             1601..2065
                     /locus_tag="ACG9BE_002421"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3033139.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2079..2828
                     /locus_tag="ACG9BE_002422"
     CDS             2079..2828
                     /locus_tag="ACG9BE_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN3033140.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2828..3658
                     /locus_tag="ACG9BE_002423"
     CDS             2828..3658
                     /locus_tag="ACG9BE_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN3033141.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3658..4305
                     /locus_tag="ACG9BE_002424"
     CDS             3658..4305
                     /locus_tag="ACG9BE_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3033142.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4403..5179)
                     /locus_tag="ACG9BE_002425"
     CDS             complement(4403..5179)
                     /locus_tag="ACG9BE_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3033143.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5323..6282
                     /locus_tag="ACG9BE_002426"
     CDS             5323..6282
                     /locus_tag="ACG9BE_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN3033144.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            6399..6707
                     /locus_tag="ACG9BE_002427"
     CDS             6399..6707
                     /locus_tag="ACG9BE_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFN3033145.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            6734..7033
                     /locus_tag="ACG9BE_002428"
     CDS             6734..7033
                     /locus_tag="ACG9BE_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFN3033146.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            7207..7758
                     /locus_tag="ACG9BE_002429"
     CDS             7207..7758
                     /locus_tag="ACG9BE_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFN3033147.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            7904..8197
                     /locus_tag="ACG9BE_002430"
     CDS             7904..8197
                     /locus_tag="ACG9BE_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFN3033148.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            8190..8486
                     /locus_tag="ACG9BE_002431"
     CDS             8190..8486
                     /locus_tag="ACG9BE_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033149.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            8557..>9459
                     /locus_tag="ACG9BE_002432"
     CDS             8557..>9459
                     /locus_tag="ACG9BE_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFN3033150.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDN"
BASE COUNT         3342 a         1357 c         1771 g         2989 t
ORIGIN      
        1 cattttatta tataaaattg ttataaaaaa atttttttga aaaatagtat tgcaaaataa
       61 agcaatttca tagataatag gtattgtaaa gttatatgac tttataatac ttattagagt
      121 gagggataaa gaaatgagac aactacacaa agaacagatt gaaacagcaa tggcagcgtt
      181 aaaggaaaag acaattgatc atgtatattt tacagcatgt gggggttcac aagccgtttt
      241 aatggctggt caatatctat ttgataaaga attaactgta ccttgtcatg tttatactgc
      301 taacgaattt aactatgcta taccaaaagg attatctgaa aattctttag ttatcacttg
      361 ttcccattct ggcacaacac cagaaactgt agaagcaaca aaaaaagcaa gagaatttgg
      421 agcaatgact atttgtttat ctaatgaaga aggatctcct ctatgggaag cagctgaata
      481 tcctgttcac tatgattggg gtaaagatgt tgatgctagt gatttaaata aaggaattct
      541 ttatggttta ttatttaata ttttacttgt tttagatggc tctaaaaaat gggaaacttg
      601 cttgaatgaa tttgataagt taaatgatct tgttattgct gaaaagagca aatttgaaat
      661 aactgctaaa gaatggggta aaaaccaaaa acgagataaa attatttaca caattggtag
      721 cggtatcaat tacggagaaa tttactccac agcagcttgc tggttcatgg aaatgcaatg
      781 gattaattct aatgctattc attctgggga atttttccat ggtccttttg aaatcactga
      841 ttatgatgta ccattcatct tagtgaaaag tattggagat acaagatttt tagatcaacg
      901 ggtagaagac ttcactaaaa aattcagtaa taaattaatg gttttagacc aagctgattt
      961 tgacttagaa tctatagata gtgtagcgaa agaatatatt gcagctgtac ttagtggcgt
     1021 tgttattcgc caaatggtag aagcgattgc ttttgaaaga ggccattcct tagatgttag
     1081 acgctatatg tggcaaatga aatattaatt aataggaggt tgcaaaagat ggcaaatcaa
     1141 ttatttcaca ttgtgattac aacacattct accctttgtg agggttattt aaaggcaaca
     1201 aaattaattt taatgaatga cgccacaggt attagtgctg ttccatttaa agaaaatact
     1261 agtccagatg attttgagga gagtatgaaa gaaataatta gtcagcatca tttgcaacct
     1321 ctaattattt taacggattt aattggagga acacctaata atgtttctat taaacagttg
     1381 aacaacaata gaattcaaat tattgctggg attaatttac ctcttttact ggagttatta
     1441 atggcccagt cagctggtaa atctatagaa gaaatagatt ttaaggaatt actgaataac
     1501 tctcaaaaaa gtatggttca tattaattcg cttttaaaag atgtcagtta aggtggcaat
     1561 ttctattcta taaattttaa aaattgaagg aggaaataga atgattaaat tagtgcgtgt
     1621 agaccacaga ttacttcatg gacaagtaat attttcttgg actaaacagt atgatattaa
     1681 ccatattatt gtggctgata acaaagttcc aggagatcca atgtctgtga tggctttgac
     1741 aattgctaaa cctgctgatt gcaaattaga cattgttaaa attagtgatg ttaaagggat
     1801 tatggaaaca tccccaaatg ataattacat gattttaatt aaaggaccta aggaagcttt
     1861 tgaattagtt caaaatttac cagaaattaa agaaattaat tttggcggtg tgtctaagaa
     1921 aagcgagtcc aaacaatatg gaaaggcagt ctttttaact cctgaagaat tagaagccac
     1981 aaaaaaaatt cttgatcaag aagtgaaaat atttgtgcaa caagtcccat cttcaagtat
     2041 tgaaaatgta gattttagta agtaaggggg gaaaataaat gttattaaaa gccattttat
     2101 taggtttagt tggtgtggta gctgttattg actccaggtt aattggtcga caaaatattg
     2161 gtagaccact aattttgagt acacttgccg gacttgtgct aggtgacttg catacaggga
     2221 ttgttcttgg ggcatcttta gaattaattt ctatgggatt cgtctcaatt ggggcggctg
     2281 gacctcctaa tatgcaactt ggtagtatta ttgccactgc ttttgcaatt ttaactaata
     2341 gtagtacaga aacagcctta acaatagcta tcccagtggc tgtagctggt gaatttatga
     2401 gtattatcat gcgcatgata attgctcaat ttggacatgc tgcagataaa gccattgaaa
     2461 ataataaatt tagaaaagct caaattattc atatttattg gtcatttatt tttaatgctt
     2521 ttgtttactt tattccaatc tttttaacag tatattttgg tgctgacgtt gtggcagatt
     2581 tagtagataa aattccagaa atgattacag acgccttgac tgtctctgga aatatgcttt
     2641 ccgcattagg gtttgcaatg ctattaagca caatgttgag taaaaaatta tatccttact
     2701 ttatttttgg attctttatt gttgcctatt caggtctttc actaattggc gtgacaattt
     2761 ttgcagctct gattgccttt gtaatggatc aagttaaata tggaaaaaga gaagaggcgc
     2821 atggttaatg gaaaatagta cagaaaaaaa acgtttaatc acaaaaaaag atcttaataa
     2881 gatgtgtctt cgttcatgga caatcatgtg ttcgtggaat tatgaaagac aaatgcatat
     2941 gggatttatg tatgggatgg cgccaatttt aaataaagtt tataaaaatg atgaagagaa
     3001 aaaaagagaa gcttatactc gtcatatggc attttttaac tgtacgcctc aaatgacttc
     3061 atttattatg ggactttgtg cttctatgga agaacaaaat gctcaagcag aagaaggaaa
     3121 ctttaatact gaatctatct ccatgattaa aaccagttta atgggtcctt ttgctggaat
     3181 tggtgattca tttttccaag gtactttaag aattattaca tttgggattg gattgtcttt
     3241 tgctaagcaa ggtagtattt taggaccgat tttagctgtt ggcctttttg ctataccttc
     3301 tattttagct gttttttacg gcggtaaatt aggatatgag tctggcaata aataccttac
     3361 taaattgtat caagaaggtc ttatggatcg agtaatgcaa tttgcttcta tcattggttt
     3421 ggctgtagta ggtggaatgg tggctagcat ggtgccaatt gtcacccctt taaaattcac
     3481 tacaggggga acagaacttg ttttacaaga cttacttgat tccattattc ctggattatt
     3541 accacttgta tgtactttgg gaatatataa attgattaag aaaaatgtta gtacaaatat
     3601 gctcttatta ggttgtattt tggcagggtt tgttttaagt ataataggta tcttataatg
     3661 attaaggctc taatttttga tatggacgga gttttaattg atagcgagat tatgtatcaa
     3721 aagtgggtat ttaatttttt aaaacaagaa aatttaagct atcctttgta ttcttatcaa
     3781 aaaaagattg gaactacagc aagtattttt gatgatatag aaaagtacaa tgagggatgt
     3841 aacagtttag aattgaaggg aaaatttact aaatattgga aatcgcaaaa gattgattac
     3901 ttggatattt ttcctaatat actaattgaa cagcttgaat ggttaaaaaa gaaaaaaata
     3961 aaaatggcaa ttgcttcttc cagtccaatg gatactataa atgaagttgt taccacttgt
     4021 aaaatagagt catattttga ttgtttaatt agcggaagag atttgccaga aagtaaacct
     4081 aatcctacta tattccttaa agcttcagaa cagctcagaa ttccagttga agaatgtctg
     4141 gtgatagaag attcttataa tggtataaaa gctggtaaaa gagcaaatat gaaggttctt
     4201 gcaattaaag ataaacgttt ttctcaagat gtaagtctag cagatgatgt tatttacgat
     4261 attaaatatc tgcgacaaga agtacaggtg aaatttggaa tataaataag ttttttgatg
     4321 tatttagaga atattagtaa aaataaaaat catgcccaaa tctaatcaaa attgattgct
     4381 ttgggtatga tttcttttaa tatcaatcta ttactttagg atctgaatca tttttatgca
     4441 ccgtatctaa agtgatattt tctgtgactt caataacata actaattcta tcacaacgaa
     4501 ctgtggattg agaataatga aatggttgat tatttttatc tttgccaacc ttttctaaaa
     4561 taaatagcgg atctcctact atacattgca ataagtcgga aagctcaggt gttgcagata
     4621 aaccgtttat ttccataata gagctcgtag ctttgacatc atagtcatct tctagaagtt
     4681 tatataatga agtatattgg ctaccttttt caaataaagt tggaaatttt tcttctgatg
     4741 cgaaaaaatt atcaattccc atcggggcac cgtcttccca cattaaacgt ttaatattgt
     4801 aaactctatc agttacattg atttgtaatt tattagctaa aaactcatca gcctttataa
     4861 ttttagaatg taaaacagta tatctaattt tatgtcctcg ggtagaaaga gaatcgctaa
     4921 atccatgaac tccagctaat tttaaacttt cttttttttg agttacaaag gtccctttcc
     4981 cccttaatcg ttcaactgtt ccactttctg ctaaaagttt aaaagctttt cgaatggtaa
     5041 tcttacttac tccataaatt ttacataatt cttcttctgt aggtaattgc tctcctggaa
     5101 gatatttttt ttcacgaata tcgttacgta tagaagcttc tacttgtaaa tataatggtc
     5161 tattatcttg aaaattcata gtacactccc taaaaagtta tacttcatta tatcacaatt
     5221 tattaaaacg tcgattgtaa aattaagcta gacaactaaa aagatcattt gtgctacact
     5281 caaaatagtt ccgaccaaag aattaataag gagtgagtac aaatgactta cacccatctt
     5341 acaacagacg aactagtaat catagagtct tatttcaata tcgagaaacc tgtcgctctt
     5401 gtcgcacaaa gtttaaatcg ctctagacaa accatttata atgtttattc gtttctcaaa
     5461 caaggaaaaa aagtgctaga ttattaccgt caatacaaac aaaataaatc tcgttgtgga
     5521 cgacgttcaa ccgttttatc tgatgaacaa tcaacatatg ttcaagaaaa ggtagctcaa
     5581 ggatggtcac ccgatgtgat tatcggccgt ggcgagtttc caattgattg ttctgttcgt
     5641 acactctatc ggatgtttaa aaaaggcctt tttgacacac agtcgttacc tatgaagggg
     5701 aaacgtaaac cgaataatca tcaagaaaag cgtggaaaac aggcctttcg cagaacgatt
     5761 catgaacgga acagtctcta tccacacctg actaaagaat ttggtcatct cgaagtcgac
     5821 accattgtgg gagttaaaca caagagcgct gttattacat tggttgagtg tttatccaag
     5881 gttattatta ctttgaaacc gacaggcaga cgagccgaag atatcgaaaa caggttaaat
     5941 gattggttca aatccatgcc taaacacttg tttaaatcca tgacatttga ttgtggaaaa
     6001 gaattttcca actggcgatc tataagtaac tcgaacgata ttgccatctt tttcgcagat
     6061 ccaggaacac cttcgcaaag agggctaaat gagcactcaa acggcttgct tagacgagat
     6121 ggcttaccta aaacaatgga ctttagggac acggatgaaa cctttattca atccgttgcc
     6181 tcgaaaagaa accatattcc aagaaaatca ttaaattatc gaacaccatt ggaagtattt
     6241 ttgagctgca tagataaaga catattgtct agcttaattt gacaaatcag atagtataaa
     6301 atgaatcaaa ttgaatatca aatatatatt atatccatga agcccaatct tctttaatag
     6361 aagattgggc tatttttatg gcaaaggagg gaaaagaaat gtttcaaaat aaaggacaac
     6421 gttatgtcac aaagggagta atggacagtt tgccggtgga attacaagct ttatgttgga
     6481 atctaatcga tcaaaacgtt caaaagcaat ttcatttaga ctatcttcaa atctttgaat
     6541 tttctacgga aaaaggaaat caaaagttgg ttcatcgtca agaagaacca gaagcaagaa
     6601 aagagtatct gatttcacct aagcttcgtt tgaaatcagt gtctcaaaaa atatgggtca
     6661 ttgattcggg tgagtatcaa acgatgttat taccagaaga atactgatat ctaaaagaaa
     6721 ggaacgaaag gacttgttag aaacggctat cgaagtactg gaaaagtgtg cacagctagt
     6781 cactactcct gaagagtggg gctatgaatc cgttacgatg gaaaaagagg aaattgaaat
     6841 gggtatgctt cctgaagatg ttactctacc acgtttagtg atgacacatc tttatattta
     6901 ttgcgcacca gaagatggaa aagactatgt agtttatttc attacagata ttaccagcca
     6961 gcgagaattt gttcgtgggt tactggtaga aggacgattg gtttggagtc agattggagg
     7021 aacgaatgaa taagatcgat tatttgagaa tgattgcgaa atgtttagaa gatatcctta
     7081 cagcgattca tcaaattctg aagaaaaaag gagaaagaat atgaaatatt ttcaactagt
     7141 attgacactt ataacggcaa tggtgattga attgcttgaa caaaaattag cataggagaa
     7201 aaagagatga ttgaaaaaga attagtgacc acacaaacag aagtttggcg agatcctcat
     7261 catacccatc gctatgtttt caaacgacaa tgggcgatca aaggaaaaga agcggaagat
     7321 aaattagcgg tagtcattac tattcgtcca acagatacag aaccatttac gaatgatttg
     7381 agtatgttat taatcgaaaa gaatatccga caattaggat ttactggttt tattgctgtg
     7441 aatctttttt cgtatacaaa agcgaaaagt cctcgggtat ttcctcgtgg aaatgatgaa
     7501 caatctttgg aaatattaac aactgttttc acagaaaaga aaatcaacac cattattttt
     7561 gcttgtggtt cgattacagc gacgagtcaa gtggcttatg aacaagcgaa gaccatttac
     7621 gatcaattga gtgcgaaaca aaaaaagatg accaaagtat tgataaatgc agaaggtaag
     7681 ttatcccacc cactaagtat tcatgttcgt aatgaatggc aattggcaga tagtcaatta
     7741 ttatttcaag aaaaataaca ttttattttc ttatgttact tataagtagt tttacatgct
     7801 ttttgacttt aaaaatacat gtgatgatac atgttatatg aattatcaat gtacccagta
     7861 atatgctatt ttcattatgt aaaaaataac agggggtgtg tagatggaga ttgatgaaac
     7921 ttgtttgagt aactcttata aaagtatgaa tgactttttt gaggaaatgg aactggattc
     7981 agaattgctc tatcaatttt atcttgcttt tcgagggcaa cagatttctt ttccaatgaa
     8041 aatgtacgat cgtgagttag ttagaaaaag aatcgaaaac atgatagaac aggaaaaaac
     8101 tgtagatatc cgtcaattga cagaagttta tggtttctct gcaagatgga ttcaaaaagt
     8161 aattaaacaa aaagaaagga gacgagtaca tggctaagct gattgtatta ggtggtttaa
     8221 tttacggtgg atattggtta tataaatata tgcttgcgca tccgaatata gataaaggaa
     8281 aagtaattaa gacagttggg gtaatcatag ttggactcat agctctaact aagaaaaata
     8341 atactaatag cccaaaaata atgagaactt atcgctgtag aggatgtgga agggaaattt
     8401 ctactagttt taatccatct gaaagccctc aaggaagaga tcgttgtcat gctggctatc
     8461 gacattcttg ggaaaggatt aactaaatga acaccattga gattaattga ctgataaaat
     8521 aagtacgcga gggagaaatt gaaaggggga aataaaatga aaaaatggtt gttattacta
     8581 attggaatgg ggattttatt cactggttgt acatctgaga atacgaggac taaagagact
     8641 gtagaaactg atacaacaat tgaggctaaa aaaagtaaaa cgcaatcaac tgaacaaact
     8701 acaacagaaa tgaaacaatc tgtggaagca agtacaagtg aaagttcaat cattgaatct
     8761 tcttctgaag aggttaaaaa tactttgtgg gatagtgaga aatctactca actagcaatt
     8821 tttgtcccac aatggggaaa gacattaggt caagaatata aaagctacaa tcctcagaac
     8881 aatgtaagtc tgtatggtac gcctttacca agtgcagtta ttgatggaaa ttggaaaatg
     8941 gcaatcaatg aagcaccagt taccgtacaa tggtcagaag atggaactgg tcatgcagat
     9001 ttcaatttag tagcagttta ttctgatgta gaaactggag aatatttagg gcagcatgta
     9061 tatttctttg gctttcaaaa tggacaacct aaagtttatt taacgcaaca aaatcaaggc
     9121 aatgataaaa actacatgta cttcaatgaa actcagaacc aacagttaaa acaaggtttc
     9181 attgatattg tgaatggaca aactcctcaa actccagtag tagaagaatc tactcaacaa
     9241 gcaacaacta caacgacaca atctacaagt agctcagcaa tttcaagttg ggaagctgct
     9301 aaagaaactg tactaaataa taaagcttta tggttgtatg attctcctga taaaaatgca
     9361 tcaaatacta atttaattag cacaaatgaa tatatgaaag atctaatgca agatgaaaat
     9421 ggaagctact attcaattaa tattgtaacg gatgataat
//
LOCUS       DAZAID010000103         9419 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000103 DAZAID010000000
VERSION     DAZAID010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.106"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACG9BE_002433"
     tRNA            2..74
                     /locus_tag="ACG9BE_002433"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACG9BE_002434"
     tRNA            78..150
                     /locus_tag="ACG9BE_002434"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACG9BE_002435"
     tRNA            155..225
                     /locus_tag="ACG9BE_002435"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACG9BE_002436"
     tRNA            238..311
                     /locus_tag="ACG9BE_002436"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACG9BE_002437"
     tRNA            323..394
                     /locus_tag="ACG9BE_002437"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACG9BE_002438"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACG9BE_002438"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFN3033151.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACG9BE_002439"
     CDS             1983..3455
                     /locus_tag="ACG9BE_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFN3033152.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACG9BE_002440"
     CDS             3538..4326
                     /locus_tag="ACG9BE_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3033153.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACG9BE_002441"
     CDS             complement(4393..5061)
                     /locus_tag="ACG9BE_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFN3033154.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACG9BE_002442"
     CDS             5176..6888
                     /locus_tag="ACG9BE_002442"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3033155.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="ACG9BE_002443"
     CDS             6891..8663
                     /locus_tag="ACG9BE_002443"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3033156.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8745..>9419)
                     /locus_tag="ACG9BE_002444"
     CDS             complement(8745..>9419)
                     /locus_tag="ACG9BE_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3033157.1"
                     /translation="GLKYNPVNGVSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKS
                     SHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYELKIKTTTKNTGS
                     YRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELN
                     LDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEK
                     LGKIEI"
BASE COUNT         2828 a         1579 c         2039 g         2973 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc agaaaagggg caatttaaat ttctattttt
     8761 cctagttttt ctactagttg attttgcata tttttagttg tatgagtata aatctttaat
     8821 gttgtttttg aatcttcatg tcctacacga tccataatgg cttttaatgg gattcctaat
     8881 tctgtaagta gagaaatgtg ggaatgcctt aacatgtgag aagaaacttt tttatctagg
     8941 tttaattctt tggatactgc tcttaaagat tgattatatg cttgaataga tatcgggtta
     9001 ccatttttag agatgaaaat gtatttatct ttattattct taattaattt gttttcttca
     9061 attaattgat ctatgatttc tttagcatta cttgataatt caatggttct ataagaacct
     9121 gtatttttag ttgttgtttt aatttttaac tcgtaatcat aagtaccaga aaccgtaatt
     9181 ttattaccat cataatcaga ttcagtcaaa gatatcaatt caccataacg tagtccagtt
     9241 aaaaacaaaa atttagtaat taaagaataa cgtgactgtt gatgtgaact tttttgttta
     9301 ctagcaatca tttgaacttc ttcttttgat aaataaccat ctttttcttt ttttatttct
     9361 tcgtttgtct taggttttct tgctaaagaa accccattta cgggattata ttttaatcc
//
LOCUS       DAZAID010000104         9386 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000104 DAZAID010000000
VERSION     DAZAID010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.107"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..306
                     /locus_tag="ACG9BE_002445"
     CDS             112..306
                     /locus_tag="ACG9BE_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033158.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            575..874
                     /locus_tag="ACG9BE_002446"
     CDS             575..874
                     /locus_tag="ACG9BE_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033159.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(971..1405)
                     /locus_tag="ACG9BE_002447"
     CDS             complement(971..1405)
                     /locus_tag="ACG9BE_002447"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3033160.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1637..2347)
                     /locus_tag="ACG9BE_002448"
     CDS             complement(1637..2347)
                     /locus_tag="ACG9BE_002448"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFN3033161.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2337..3197)
                     /locus_tag="ACG9BE_002449"
     CDS             complement(2337..3197)
                     /locus_tag="ACG9BE_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFN3033162.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3202..3840)
                     /locus_tag="ACG9BE_002450"
     CDS             complement(3202..3840)
                     /locus_tag="ACG9BE_002450"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFN3033163.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3855..4154)
                     /locus_tag="ACG9BE_002451"
     CDS             complement(3855..4154)
                     /locus_tag="ACG9BE_002451"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN3033164.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4197..5681)
                     /locus_tag="ACG9BE_002452"
     CDS             complement(4197..5681)
                     /locus_tag="ACG9BE_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN3033165.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5701..6162)
                     /locus_tag="ACG9BE_002453"
     CDS             complement(5701..6162)
                     /locus_tag="ACG9BE_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3033166.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="ACG9BE_002454"
     CDS             complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="ACG9BE_002454"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFN3033167.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7465..8235
                     /locus_tag="ACG9BE_002455"
     CDS             7465..8235
                     /locus_tag="ACG9BE_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN3033168.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8508..9335)
                     /locus_tag="ACG9BE_002456"
     CDS             complement(8508..9335)
                     /locus_tag="ACG9BE_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3033169.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2911 a         1928 c         1475 g         3072 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      481 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt
      541 tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag
      601 tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca
      661 caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc
      721 ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt
      781 tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt
      841 atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg
      901 atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg
      961 ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat
     1021 aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg
     1081 ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct
     1141 ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga
     1201 ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa
     1261 tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc
     1321 ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat
     1381 cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa
     1441 taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg
     1501 attccttaaa attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt
     1561 gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac
     1621 ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat
     1681 gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt
     1741 tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat
     1801 ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta
     1861 attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc
     1921 ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat
     1981 gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg
     2041 aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg
     2101 ggagatctga agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta
     2161 ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga
     2221 ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc
     2281 gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac
     2341 aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt
     2401 tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa
     2461 tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga
     2521 acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt taagtggata
     2581 gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg
     2641 gacaaattgc ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt
     2701 tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg
     2761 atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg
     2821 gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg
     2881 cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt
     2941 tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata
     3001 cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct
     3061 aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt
     3121 tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa
     3181 atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg
     3241 tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac
     3301 atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt
     3361 aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc
     3421 agataacagc gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca
     3481 atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc
     3541 tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc
     3601 tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc
     3661 gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg
     3721 taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc
     3781 tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat
     3841 cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg
     3901 atttctttga ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc
     3961 tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa
     4021 ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc
     4081 attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat
     4141 actaaaattc tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat
     4201 actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga
     4261 tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca
     4321 gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac
     4381 atcccgatat aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg
     4441 cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg
     4501 ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg
     4561 acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca
     4621 ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg
     4681 tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt
     4741 agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt
     4801 aaagaagtcg tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga
     4861 atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac
     4921 gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca
     4981 atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca
     5041 gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca
     5101 ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa
     5161 cctaaaatca gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac
     5221 agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg
     5281 gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc
     5341 agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc
     5401 acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt
     5461 agtccaacca agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata
     5521 aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc
     5581 aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt
     5641 gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa
     5701 ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg
     5761 ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc
     5821 ttctggatct tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc
     5881 ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac
     5941 accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg
     6001 accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt
     6061 atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc
     6121 tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct
     6181 tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga
     6241 taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc
     6301 cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg
     6361 atctcttttg gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact
     6421 tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc
     6481 cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt
     6541 ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt
     6601 tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc
     6661 tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca
     6721 ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga
     6781 attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga
     6841 caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta
     6901 acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt
     6961 aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc
     7021 atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt
     7081 ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca
     7141 ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt
     7201 tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc
     7261 ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc
     7321 ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt
     7381 ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa
     7441 gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa
     7501 aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga attagctgaa
     7561 tcccttgatg tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac
     7621 gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat
     7681 caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac
     7741 gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt
     7801 caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa
     7861 gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg
     7921 ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt
     7981 agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac
     8041 gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc
     8101 ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat
     8161 aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa
     8221 gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat
     8281 aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga
     8341 tgtatccatc caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg
     8401 cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt
     8461 tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg
     8521 ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc
     8581 atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa
     8641 agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca
     8701 cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa
     8761 tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc
     8821 tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt
     8881 gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa
     8941 taaaataatg ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat
     9001 aaaatttgat ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt
     9061 ttataagatt cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc
     9121 ctgattttgt cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg
     9181 caaagcgcac ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca
     9241 gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg
     9301 tattctttta gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat
     9361 tatttctcct ttatatcata cacaaa
//
LOCUS       DAZAID010000105         9376 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000105 DAZAID010000000
VERSION     DAZAID010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.108"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..775)
                     /locus_tag="ACG9BE_002457"
     CDS             complement(107..775)
                     /locus_tag="ACG9BE_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN3033170.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            909..1196
                     /locus_tag="ACG9BE_002458"
     CDS             909..1196
                     /locus_tag="ACG9BE_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033171.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1612..3150
                     /locus_tag="ACG9BE_002459"
     CDS             1612..3150
                     /locus_tag="ACG9BE_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFN3033172.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3147..4112
                     /locus_tag="ACG9BE_002460"
     CDS             3147..4112
                     /locus_tag="ACG9BE_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3033173.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4099..5421
                     /locus_tag="ACG9BE_002461"
     CDS             4099..5421
                     /locus_tag="ACG9BE_002461"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN3033174.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5484..5588
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5662..6405
                     /locus_tag="ACG9BE_002462"
     CDS             5662..6405
                     /locus_tag="ACG9BE_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3033175.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6420..7412
                     /locus_tag="ACG9BE_002463"
     CDS             6420..7412
                     /locus_tag="ACG9BE_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN3033176.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7424..8161
                     /locus_tag="ACG9BE_002464"
     CDS             7424..8161
                     /locus_tag="ACG9BE_002464"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3033177.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8151..8873
                     /locus_tag="ACG9BE_002465"
     CDS             8151..8873
                     /locus_tag="ACG9BE_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFN3033178.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8957..>9376)
                     /locus_tag="ACG9BE_002466"
     CDS             complement(8957..>9376)
                     /locus_tag="ACG9BE_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFN3033179.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3076 a         1608 c         1973 g         2719 t
ORIGIN      
        1 tgcaaaaata gtttattttt ttataaagaa gattttactg aataatatca ttttagttta
       61 cataatgata tttctgtaaa ttaaataaag tagtgaaaaa taagaatcag tgctctaact
      121 gttcaagagg atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc aattggggca
      181 aaacagatcc aatttccaga tacttcactt tattcaatgt ataagataga atagaacagc
      241 taagtaatgt atacagaaaa tgtgtgagat ccaaataaag caaggagaca actaaaatac
      301 caaaagtaat caagaaaaga cttttgccaa tcttaatatg aaaatatttt tcaatggctt
      361 gataaatgat atctgttccg cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa
      421 tgccagaaag aatacctgtc atcaaactat tgatcagtag actatctaca gcaaaatgta
      481 tatgaaggct ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc
      541 ggagaaaagc gtgttttcca aaaagaaaca atgagataag caataaaggt aaattaaaaa
      601 gcagtgaagt gacagaagga gcgaatccaa aaagattatg caaaagcaaa gcaataccag
      661 taaccccacc atctgccagc ttagatggaa tattgattcc tgtcacagct aatccgtaga
      721 ttcctgcgcc taaaatgatt ccgcacaatt ctgtaaataa atttaatttt ttcatttcta
      781 gcctcttttc ttttgaaaat tttcagcaac ttctttatta tactgactta tactcttact
      841 tcaagaggaa aatgaaaaaa aattattttt ggctgtttct ttttatttat caaataagga
      901 gtggaaaaat gcgagcagca gacttattga caattcttac aaaatcaccc aaaaaaacag
      961 agtatcaagg agtttttttg gctcaagaac aaaaactggt gcctgtgaca atggtccaga
     1021 taaataaagc aaatcaattt atctttttat ttgaacacgg caaagcacca cttgctatga
     1081 aagacatctt tctttttttg atgaagaacc gtgagaaagc tgtttattat cagtcaaaag
     1141 aagaactcat tcctctttat ggagtgaaag agatagaaaa taaagtggtg atataagtaa
     1201 aaataagaaa acaagacgtt tatgatgtca aaacaaccat tttttacatt tttggcaaaa
     1261 aagtgtcatg atacagtata atattcgttg aacaggccaa taaaggtgtt ctcccccacg
     1321 agatttctac tttattggct gtttcttttt attaaaataa aagttgtgtg tgtaaaaaag
     1381 atatagctga ttccaagtga caatttcgta tagattttta tggtattcgt agtgaaaaaa
     1441 cgagaaccaa aaagttgatc cgactttgtt gcttggattc tttttttgtc agcatattta
     1501 gaggtatttt tttctttttt ttgttcgtgc tgtaaggtta aggcaagaca agttagttgg
     1561 gaaaaaggaa agaaatgttt atacttaaag aataacagaa aggaaggtcg catgaataat
     1621 tttatacaaa cattaagtga aagaaaaggc gagttgctgg aagctgcatt tcaacatctg
     1681 tccatttctc tcatttcttt gctgattgca gcactgattg ctattccttt agcgatttgg
     1741 gcagccaatc ataaaaaaat ggcagaagtc ttattgcaga tcacgggtgt gctccaaacg
     1801 attccttcac ttgccttatt aggtttactt atcccatttg tagggatcgg aactgttcca
     1861 gcattgattg cacttgttat ttacgctttg cttccgattt tccaaaacac gtatattggt
     1921 ctagctgaaa ttgacccgtc tattgaagaa gcagcggtag catttggtat gtccagaatg
     1981 agacgcttgc tgaaagtaga gcttcccatc gcattacctg tcattatctc aggtatccga
     2041 actgccttag ttttaatcat cggtacggct actttggcag cattgatcgg agcaggtgga
     2101 ttagggacat ttattttgtt aggtattgac cgaaatacac ctgttttgac attgattggt
     2161 gcgatcagtt ctgccttgct tgcaattata ttcagcggac tgattcgctg gttgcagcat
     2221 aagaaacctc gatattccat cattacttta gcagcagtgg tgtgtttatt tggcggcgtt
     2281 tctttatatc agtctgcctt gtttaaaaat gaaaccatca cgattgctgg aaaattagga
     2341 gcggaacctg aaatcctgat ccaaatgtat aaagaattga ttgaagacaa cacaaaaaca
     2401 gaagttgaat taaaaccgaa ttttggcaag acgagttttt tgttcaatgc actggatcaa
     2461 ggtcaaatcg atatctatcc tgagttcaca ggtactgtcc tagaaagctt ggtgaaggta
     2521 cctgaagcaa tgaaaagtgc tgatttgaca aaagaaaaaa catatgacga agcagaagaa
     2581 ttattgaata agcaattcga tatgacatta ttaaaaccaa tggcttacca aaatacgtat
     2641 gccttggcag tcaagagatc ttttgcgaac gaacatcaac ttaaaaagat ttccgatttg
     2701 gcagctatct ctgatcagat caaagcggga ttcacactgg aatttattga tcgttcagat
     2761 ggatataaag gaatccaaga agcatatggc ttgaattttt caagtgtaca aagcatggag
     2821 ccaagtttgc gttaccaagc aatcaataat ggagatgtca atattgtgga tgcctattcc
     2881 acagatagcc agctcaagga gtacgatcta gtgactctag aagatgatcg agggttattc
     2941 ccagcttatc aaggcgcacc gctgatgaaa aaagattttg ctaaagaaca tcctgaaatc
     3001 gttaaagcac tgaataaatt aagcggaaaa ataacagagg atcaaatgat tgatatgaat
     3061 taccaagtca atgtcgaaaa gaaaaagccg acagaagtag cacgaatctt cctgaaaaat
     3121 gaaggattag taaaggagga agatcagtga ctaaattaat cgagttcaaa catgtgcaaa
     3181 aaaagtatga tggaaagtat gtcatcgatg acctaaatct ggcaatcaaa aaaggagaaa
     3241 tctttgtttt ggttggtcct tccggtagtg gaaaaacaac tactttgaaa atgatcaatg
     3301 gattatctaa accatcagca ggagacatct acttcaaagg aaaatcgtta aatgaatata
     3361 atctgcagaa aatgcggtgg aatatgggat atgtgctgca gcaaatcgct ttgttcccga
     3421 ccatgacagt caagcaaaat attgaagtca tccctgaaat gttaggttgg gaaaagcaga
     3481 aaagagcaga ccgtgtagat gaacttcttc aaaaagttgg cttatctcct gatatttatc
     3541 gagatcgtat gcctagagag ttgtctggag gagaacagca acggatcggg atcatccgag
     3601 ctattgctgc aagcccagat gtgattttga tggacgagcc atttagcgca ttagatccga
     3661 tttcaagaaa ctcattacaa gaattggttt tgtccttaca cgaagaattg ggcacgacca
     3721 tcgttttcgt tacgcacaat atggaagaag caatcaagct aggtgatcgt attgcattta
     3781 tgaaggatgg agaaattatc caatgtgaca caccagagca gttattgatg aatcctaaaa
     3841 acgactacgt tcggcatttc tttgatgaac cgaaacagac gaaagagtgg cgagtagaag
     3901 atttagtcgt caatggatat ttcttaaatg aaataccaga aaatgctcgt caacaagttt
     3961 gctttgatac gcctatgaaa gaagtatatt cgcttctttc ggtgtatcca agcattgtga
     4021 tagtagaaaa gcaaagaagt attgggtcgc tgacaagtaa agaaatcttt gcttttttaa
     4081 gtcgagagga ggaaggaaat gaaaacatat gatgcaatca tcatcggttc aggtgtgagc
     4141 ggcttaagtg ctgcttatgg attgaaagaa gcaggtaaaa cagtactagt ggtcgaagaa
     4201 gatctatggg gcgggacttg tcctaaccgt ggatgtgatc ctaaaaaagt cttgttaagt
     4261 gcagtagagg cacgtaatcg ggtaaaacaa ttgagtggaa aaggttttaa cgagatccca
     4321 actgccaact gggaagaact tcaaaaattc aaacgaacat ttacggatcc agtcccagaa
     4381 agccgaaaaa aacaattagc agaagctgaa atcgatcatc tttcaggaac cgctcgtttt
     4441 ttggatgatt catctattga agtcaacgaa gaagtcttcc atgcggatta cttagttttg
     4501 gcaaccgggc aaagacctac gatcttacca gtggagggaa aggaatactt gaaaactagt
     4561 gcagactttt tatcattgcc tgtactgcct aaagaaatca tttttatcgg tggtggttat
     4621 attgcttttg agctagctac gattgccaat gctgcaggga gcaaagtaac cattgtccat
     4681 cataatcaac ggccattaaa agaatttgag gcgtctttgg tagaggaagc cgtccatcaa
     4741 atggaagcat ctggcattca gtttgctttt ggtgttgaaa cccaaaagat catttcagaa
     4801 ggtactcgct atcgtttagt cggaaaagag acggaattag tagcagatat gattttttgt
     4861 gcgactggga gacagccgaa tacagaatct ttagcacttg agcaagcgaa tatcgtcttt
     4921 gataaacacg gaattgcagt caatgattat ctgcaaacga gcaatccaaa aatttttgcg
     4981 tgtggagata ttgtttcaag gaaaacccca aaattgacac cagtcgccac ttttgaagga
     5041 aattatgtag ctaagcggat aacggatgca acaagcgaac ctatcaaata tccgattata
     5101 cctacgatcg tctacgccag tcctaaactt gctgaagtag gagttacaaa aagtcacgcc
     5161 tcttcgtcag atcaagtggt agagatggat cttaccagtt ggttcaccta tcatcgtgtc
     5221 aatgaaccag ttgctaaggc tgaattgacc tttgaccagc aaaattattt gattggtgct
     5281 gcagtgatta gcgagcaagc agacgaattg atcgatgatt tgacactcgt catcaatcaa
     5341 aaattgacta aaaaagagtt ggatagctat atcatggggt atccgacatt ggcaagtgat
     5401 ctctcctatt tattgaaata atcagcatga taactaaagc gcttgttgct ttttccttct
     5461 tgtgctacac tttaaaaaat gaatacctgt ttctggggat gtcgtagacg acatgagagt
     5521 aagttgtgaa cttagaccca ttgaacctgt cagttaatac tggtgaaggg agaaacatag
     5581 cagacgcttg aagcgggtta ctatgtcgtg cccatttcaa gcgttttttg ttattcatat
     5641 tagaagaaag gaggtaacaa aatgaagaaa aatctttatc cagcagtcag tatggcattc
     5701 attttaatta tttggcagat tgttgtcata gtttggaaaa tacctgcctt tattttaccc
     5761 ggacctgctg ctgtattttc tgcattagcg gcagatgcac aagtattact ttcccattct
     5821 gtcacgacat taaatgaagc agtgcttggt ttggtaatag ctagcgttct ttcttttatt
     5881 actgccttgg caatggatta ttggaagttt ttagaaatgt ctttttatcc gttactagtg
     5941 atttcacaaa tgttgccaat catggtgtta gggccattac ttaccctatg gtttggtttc
     6001 ggtatgcttc caaaagtgat ccttgttgtc ttgataagct attttccgat cgtagtttct
     6061 ttttcggata cattgaggaa agtatcaaaa gagcaaatca tctttttgaa aacgatgggg
     6121 gcagatacag ggaagattta tcgaatatat aaaataccag cgggtttatc cggcttcttt
     6181 tccggtttaa aagtggctgc tacttattgt gtcagcggag cagtcgttgg agaatggcta
     6241 agcgctcaag caggcttagg gtattacatg atacgcgtaa aaaacagcta ccagatcgat
     6301 aaagtttttg ctgcgatcat ttgtgtcatt ctattgagtt tattgctgaa tggcgcttgt
     6361 tcattattga aaaaagggta ttatcgtatg ctttatattt tttaagagaa ggggatcaaa
     6421 tgaaaaaatt atggctttta ttaccgctac tgttgctttt atccgcttgt ggcactgcaa
     6481 aagaaacaag tagcaaacaa gaaataaagg atttgaaaaa agtgacactt gttttggatt
     6541 atgtaccgaa tactaatcat actggcatct acctagcaaa agaaaaaggc tattataaag
     6601 aggctggttt aaatgtccaa atcatcgaac caggagataa ttcgacaagt attggtctag
     6661 taggtgcgga caaagcacaa tttggtgtaa gttatcaaga agatgtcact tatgctcacg
     6721 cagatggaca gaatatacca gtcaaagcaa ttgctacagt gatcaaacat aatacttctg
     6781 gattcgccac cctatcagac agtaacatcc attcaccaaa agattttgaa ggaaaaacat
     6841 atgcaggttg gcaaagtcca agtgaagaag ctgtcttaaa agctgtgatg gaaaaagacg
     6901 gcggggactt ttcaaaactg acaatggtag gaagcaatgg agaagggcca gaaagcttag
     6961 gaaaatcctc agatatccaa tggtactttg aaggttggga tatgatcaaa gcaaaagaag
     7021 cgggtattga actaaactat atcccattga aagaattaga tgaacggtta gattactata
     7081 ctccggtaat tattacgaat gatcaactga tcaaaagtga tccagaattg gtacagtctt
     7141 ttatggatgc cacgaaaaaa ggctatcagg aagctattaa ggatccaaat gacagtgcta
     7201 aactgttgca aaaatatgca aaagaaaatg atcggacgtt tttagaagaa tcacaagcat
     7261 tcttgtcaaa aaattatacg gatgatcctg agaattgggg attgatggaa gaaaaagttt
     7321 ggcgcaatta tacagctttt atgcaggaaa acggattgat caagcaagat gtaccttcgc
     7381 aagaactatt cactaatcag tttataaaat aggagtgata gatatgtcaa tccaaatcag
     7441 ccacttgaat gtagtcattg aaaacctgaa aatattagaa gacatccatt tcactatccc
     7501 taaagatacc tttacatcga ttgtcgcgcc aagcggagcc ggtaagtcaa cattgctgaa
     7561 aactttaaca ggagtccagc ctatgacttc tggaacgatc aaagtagacg atcaaaaagt
     7621 aactggtctc catacagcat ttagttatat gccgcaagaa gatatgttac ttccttggct
     7681 gagcgtctat caaaacgtta cgttatatca aaaaatcaat catctaacaa tcaatgaaga
     7741 acaagtaagg gattatctgg atatctttgg gttaaacggc tatgaacatt ttttgccgga
     7801 acaattatct ggcgggatga agcagcgaac ggccttgtta cgaacaatca tgaatccttc
     7861 ttcttacttg cttcttgatg aaccatttgg agcgttagat gctatgacca gagggcagat
     7921 gcaagactgg atgttgaagc tgcctaaaaa agcaaagcgg actactttac ttgtgacgca
     7981 tgatatcgaa gaagcaattt atttatcaga caggattctt gtgctttccg cccgtccggc
     8041 acatgtgatt gctgagattc aagtgcctga aaaacgacgg tcacgtgaat ggctactgca
     8101 gcaatcagag ttgaagcaaa cgatttatca gttattggca ggtgaattag atgttaagtg
     8161 atgctcattg tcatttagat gggagtcaga gtttagcact ccttcagcag gaacacacga
     8221 ttttgacgat catcaattgt gattcgccag aagaatggaa ggaaaacaga cagcttgcag
     8281 caagtaagac tcaagcctta agttacggta tccatccttg gaaagcagat agctatacgt
     8341 ttgaacaagt tgagcctttt ttgaaaaaag caaggatcat cggtgaaata gggttagata
     8401 atatttggac gaatgtaccg atgactacac aaaaaaaagt atttgagcga cagcttgctt
     8461 ttgcagccat aaatgagaaa cctgttgtac tgcacacaaa aggatgcgaa aaagagatct
     8521 taaattatat ccaagcttac cctaatcact atttgatcca ttggtattct tctttggact
     8581 atcaacaaga ctatattgat ttaggctgtt atttcagcat tggtcttgat ttgaaaaaga
     8641 atccagcagt ctggcaacta gctaaagcag tcccaatcga ccacttactt attgaaacgg
     8701 atggactaca tgcagcagag tgggctttag gggggatttt ttcagaaaaa gattatgtct
     8761 cactgcttga agaacagtta aagacaatcg ctgagatcaa agagctatca gttgaagaag
     8821 ttaaaaaaaa tacttatcaa aacttggaac gtttcattag attaggtaaa taaaaaagag
     8881 atgtgacaca actagtgtcg cacctctttt tggctttctt tgtataaaag taaactgttt
     8941 gattaataaa tgaatcttag taccaaccgt ttgcttccca gaatgctttc gccgcttccc
     9001 aagaaccata acgtgaagta acatattgtt ctgctactct ttcttggttt gcagcagagt
     9061 agtcaccatt taagtaagaa gaatctaatt ggtaacgtcc gatgtaacgt ccgttcgttg
     9121 ctgtgtaaga accgcttgat tctttttgtg cgatccattc ttttgcagag cttgtgcttg
     9181 ctggagtagc agcagtttct gttacagcag gagcaggttg tgtttcagca acaggcgctg
     9241 cttgtttttc cgtttgaacc gtttcttgta caacaggttg ttcagcttgg actggtgctt
     9301 cttgaacggc tgcttgaggt tcagcagctg cttgtgcaga gccttctgtt gggattgtta
     9361 attgttgtcc tgagta
//
LOCUS       DAZAID010000106         9322 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000106 DAZAID010000000
VERSION     DAZAID010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.109"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..107
                     /locus_tag="ACG9BE_002467"
     CDS             <1..107
                     /locus_tag="ACG9BE_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFN3033180.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="ACG9BE_002468"
     CDS             complement(176..904)
                     /locus_tag="ACG9BE_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN3033181.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="ACG9BE_002469"
     CDS             complement(917..2197)
                     /locus_tag="ACG9BE_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN3033182.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="ACG9BE_002470"
     CDS             complement(2338..2940)
                     /locus_tag="ACG9BE_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFN3033183.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="ACG9BE_002471"
     CDS             3185..3391
                     /locus_tag="ACG9BE_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033184.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="ACG9BE_002472"
     CDS             3703..4422
                     /locus_tag="ACG9BE_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN3033185.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="ACG9BE_002473"
     CDS             complement(4503..5186)
                     /locus_tag="ACG9BE_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFN3033186.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="ACG9BE_002474"
     CDS             5405..7591
                     /locus_tag="ACG9BE_002474"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFN3033187.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            7937..8347
                     /locus_tag="ACG9BE_002475"
     CDS             7937..8347
                     /locus_tag="ACG9BE_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN3033188.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            8361..8717
                     /locus_tag="ACG9BE_002476"
     CDS             8361..8717
                     /locus_tag="ACG9BE_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN3033189.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         3364 a         1525 c         1745 g         2688 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga ggtgtgacaa
     7741 gaattttgtc acacctccct tatttatttt gaaaatgaga atactattat caaaatagag
     7801 aatatataga aaaattctca ttattcaaac gtaaataatc agttattttg atttttttta
     7861 tattttatta gctaataatt ggtattttaa aagaaattac tgtagaattg gattataatt
     7921 aaaaaggagt gagaaaatga taaaagtata tacttcgcct agctgtactt cctgtagaaa
     7981 agcaagagca tggttgcaag cgaatcaact tgaatttgaa gagaagaata tttttgccga
     8041 accgttaaca gaaagtgaga tcaaaaagat cttggcagcg actgaaggag gagttcaaga
     8101 tattatttcc actcggtcga aaatttacga aaagctgggc atagatttta acgaattaac
     8161 tttaaagcaa atgatcgtct tattcaaaga atatccttca ttattgagac gacctatttt
     8221 gattgatgaa aaaagaatac taatcggatt taatgaagat gagatccggt catttgtccc
     8281 tcgtgaaatt cgatgtgttg ccagaaatct gatctattta caaaacaata acatcttaaa
     8341 ttcataggga gggagccttc gtggaagtga aagtctatgg atcagcgcac tgtgtaacaa
     8401 gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc ttatcaattt gttgacttag
     8461 aagaacgcga tttaagtaca gaagaagcag aagaattatg cagttttgaa gaagtgcaag
     8521 cagaacagct atttgcgact tggtctgagg cttttcacaa cattgcgatt gacctttcct
     8581 gtgtaggaaa aaaacaactg atttttttat gcacacaaca taccaatctc ctcagacgtc
     8641 ctttgattat tatcgatgaa gttctattta ttggatacaa tgagcagttg atggaacagc
     8701 ttatcttaaa taaatagact ttaactgttt taatcaagtc actgagcatt tcatacagtt
     8761 gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc tttttttgac tttatttttc
     8821 tactatagag acatgctcaa tttatgtggt caaaattcaa atctaggtgg ggatatagag
     8881 ttaaaaatat attgacatgt gtaaatttac atgatatatt catttctgtt gttttaagta
     8941 gcaaagcaat aaaatagtat tttaaaaaac atataaaata gttgttgaca tgttagaaat
     9001 gacatgataa gataactgag ttgttaaaaa agaaccactt gtgaagcttg attctatcga
     9061 aaaaaacttc aaaaaagttc ttgacaaata acaaatgata agttatgata aatgagttgc
     9121 tgtaagattt cgaaaaacga aataaaaaaa acttttaaaa aagagttgac aattacttgt
     9181 ctctctgata tgatataaaa gttgctacgg cagccaaaaa cgaatcgggg aacactcctg
     9241 atgaagtaga cctttgaaaa ctgaacaaag taagacaaac caaatgtgta gggcgtcttg
     9301 attcaattca agacaacaaa ca
//
LOCUS       DAZAID010000107         9293 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000107 DAZAID010000000
VERSION     DAZAID010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.110"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG9BE_002477"
     CDS             <1..435
                     /locus_tag="ACG9BE_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFN3033190.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG9BE_002478"
     tRNA            complement(759..842)
                     /locus_tag="ACG9BE_002478"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG9BE_002479"
     CDS             1370..1870
                     /locus_tag="ACG9BE_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN3033191.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACG9BE_002480"
     CDS             complement(2060..2404)
                     /locus_tag="ACG9BE_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFN3033192.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACG9BE_002481"
     CDS             complement(2558..3478)
                     /locus_tag="ACG9BE_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN3033193.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACG9BE_002482"
     CDS             3665..4156
                     /locus_tag="ACG9BE_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFN3033194.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACG9BE_002483"
     CDS             complement(4180..6555)
                     /locus_tag="ACG9BE_002483"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFN3033195.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ACG9BE_002484"
     CDS             6881..7507
                     /locus_tag="ACG9BE_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN3033196.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ACG9BE_002485"
     CDS             7530..8234
                     /locus_tag="ACG9BE_002485"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN3033197.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ACG9BE_002486"
     CDS             8426..9259
                     /locus_tag="ACG9BE_002486"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFN3033198.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2833 a         1791 c         1654 g         3015 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct tatttgagaa atg
//
LOCUS       DAZAID010000108         8940 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000108 DAZAID010000000
VERSION     DAZAID010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.111"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1152)
                     /locus_tag="ACG9BE_002487"
     CDS             complement(13..1152)
                     /locus_tag="ACG9BE_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFN3033199.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACG9BE_002488"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACG9BE_002488"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFN3033200.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACG9BE_002489"
     CDS             complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACG9BE_002489"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFN3033201.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACG9BE_002490"
     CDS             complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACG9BE_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFN3033202.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACG9BE_002491"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACG9BE_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFN3033203.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="ACG9BE_002492"
     CDS             complement(6166..7257)
                     /locus_tag="ACG9BE_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFN3033204.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACG9BE_002493"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACG9BE_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFN3033205.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="ACG9BE_002494"
     tRNA            8800..8871
                     /locus_tag="ACG9BE_002494"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
BASE COUNT         2317 a         1897 c         1647 g         3079 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
//
LOCUS       DAZAID010000109         8890 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000109 DAZAID010000000
VERSION     DAZAID010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.112"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(382..879)
                     /locus_tag="ACG9BE_002495"
     CDS             complement(382..879)
                     /locus_tag="ACG9BE_002495"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFN3033206.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(919..1728)
                     /locus_tag="ACG9BE_002496"
     CDS             complement(919..1728)
                     /locus_tag="ACG9BE_002496"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3033207.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2062..2919
                     /locus_tag="ACG9BE_002497"
     CDS             2062..2919
                     /locus_tag="ACG9BE_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFN3033208.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2912..3814
                     /locus_tag="ACG9BE_002498"
     CDS             2912..3814
                     /locus_tag="ACG9BE_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFN3033209.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3847..4197
                     /locus_tag="ACG9BE_002499"
     CDS             3847..4197
                     /locus_tag="ACG9BE_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN3033210.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4246..5169
                     /locus_tag="ACG9BE_002500"
     CDS             4246..5169
                     /locus_tag="ACG9BE_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN3033211.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5247..6140
                     /locus_tag="ACG9BE_002501"
     CDS             5247..6140
                     /locus_tag="ACG9BE_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN3033212.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6178..6741
                     /gene="amaP"
                     /locus_tag="ACG9BE_002502"
     CDS             6178..6741
                     /gene="amaP"
                     /locus_tag="ACG9BE_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN3033213.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6755..6943
                     /locus_tag="ACG9BE_002503"
     CDS             6755..6943
                     /locus_tag="ACG9BE_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN3033214.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6956..7858
                     /locus_tag="ACG9BE_002504"
     CDS             6956..7858
                     /locus_tag="ACG9BE_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN3033215.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7890..8132
                     /locus_tag="ACG9BE_002505"
     CDS             7890..8132
                     /locus_tag="ACG9BE_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN3033216.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8388..8759)
                     /locus_tag="ACG9BE_002506"
     CDS             complement(8388..8759)
                     /locus_tag="ACG9BE_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033217.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3099 a         1535 c         1721 g         2535 t
ORIGIN      
        1 gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc
       61 ttatgttttt acttggctgt tcttattact gcagatgcaa gcttttgaat accgtttaag
      121 gaagaggttg tgaaatcagc gttttgacct gagtattagc ttaacaaacg gaaaaatagt
      181 tcctcatatt ttgccgtttg ttaagctaat accgagggtc agctgattga acaccattta
      241 agaaagaggt cgtgagcctg ccgtttcact ctgtgtcata aaaaagccaa tctactttga
      301 tagaaaaagc acgtaggact aaaacagttc tgcgtgcttt ttttattgtg tgttaaatat
      361 cgtaaatgaa gatagatatt tttatgattc gacgatagag acgttgaaat ctttcaacag
      421 ctctaaagcg gattctccgt gttttctact ggagactaac attggatcaa caaaaaagtc
      481 tgcttcagga aaagcactct gcaggcctat cagattagag agtacacaca agtttgtttc
      541 aatcccgact acttcgatct ctttttgttc ttctttgctt tcaaataaag tttcctggat
      601 ttcagctaaa gtttcaggtg gaatcgtaaa gtaatacttt ttaatcactc gttcattttc
      661 ttgtggagaa agaagaggga tcaattgcaa atcttctcgt tcttcttctt tgtccttcag
      721 ttcaactggt atatcccgtg tgaagagaat atattcatta ttttggcgtg ctttagctaa
      781 tctttgagca atacgttctg ccaattgatc tgactctgga atataaaatt cgctgtttgg
      841 atcaagaata tgattttgca tatcaataat aactaacatt ttctttcctc ctacattcat
      901 atcgtcattt tattcttttt aatagaagtt ttttaaaata taagcaacac cagcttcttc
      961 attcgtcagt gttacttggt ttgcaatttc ttttacttgt cgctgtgcat tccccatcgc
     1021 aatactcaac ccgctgtaat tcaacatagc aatgtcgttt tcttgatcgc caaaagccat
     1081 gatgtcactc gattcgattc caaggtaatc cgccagaaaa acaagtgaat gagctttagt
     1141 gatacctgta ggcgtgattt ctaagaaatg cggatcagag aatactgatt ggataccatc
     1201 tgcttgcaga tctctttgta gcttctgtag tttttcttca ctcttatctg ttaatgctaa
     1261 tttaagatag ctaagagaag gatcttccaa catatttaaa atttgatcaa aagaatggtt
     1321 ctgtaaattg aaatgttcat aataagggtc atctttgact agatttgatt catcgaatgt
     1381 cagctgatca tcttgggttt ctcggctagc aatgatggat attttcgctt cttgagcata
     1441 gaagaaggct tttctcagtg tatcgctatc tatttttgaa cgtctgagaa ctttcgccat
     1501 ttttccatcc ttcatttcag caatcagtga accattggaa gaaatcaaat agccaccgat
     1561 tcctaattcg gaaaaaatat ctttagcaga aatgctgttc cttccggtag cgacggttac
     1621 gattccgcca ttttcatcaa atttccgaag tgcttggata ttttcttctg gtatctttcc
     1681 atttcgatca agcaaggtac cgtctaaatc aatcgctgct agtttcattt tatcttccta
     1741 ctttcttttt acatatctat tttcctgtta ccttaagatt aaatgagaat ttaaaaaaat
     1801 gaaactaact tgctttgtca ttatgaattt tttcaaaaaa cattcttaag aaaaaggtgg
     1861 ctatatatag tagtttttgg tcaatacctt tgaataaaaa gccatgaaaa acgaatagtt
     1921 agcgcttacc gatgcgtttt gtgataaaaa aattttaaaa tattcaatga aaacaaagca
     1981 tatattttgc tttaaaacca aatgaggaat attctagatg tagaacaggt tagaaaaaga
     2041 ttagaaaaag gagtgatttt gatggagaag agatacggcg tagttcctgt ggtaactgct
     2101 tcagacgaaa attatgctcc ttacttgagc gtgatgatcg caactgcatt agaaaattgc
     2161 aataaaacaa gacgcataaa attttatgtg attgacgatg gtctatcaga atatagtaaa
     2221 gaaggattgg aagaaacggt aaataaatat tcaagtaatg caagtatcca attcctgaca
     2281 gtggaaaaag atatctacga agacttccta gtcagtgacc atatcacaac taccgcttac
     2341 ttaagaattt ctttaccaaa cttattagct aaagaggatt ataagaaagt cctttactta
     2401 gattcagatg tgttagtact agacgatatc gtaaaactat atgatgagcc gttaaatgga
     2461 aaaacaatcg gcgctattat cgatccaggc caagtgaaag ctttggaaag attaggaatc
     2521 gattccgatg acttgtactt caattcagga gtgatggtga ttgacattga tcaatggaat
     2581 aaaaaagaaa ttacagaaaa aactatccat tacctcagtg aaaatggcga tcgaatcatc
     2641 tatcacgacc aagatgcttt gaatgctgta ctttatgaag attgggaaca acttcatcca
     2701 aaatggaaca tgcagacttc gctgattttt gaacgtcatc cagcaccaaa tgaaaaatat
     2761 gaacgtcagt ataaagaagg aaatgaaaaa ccttcgatcg ttcactttac aggacatgat
     2821 aaaccttgga atacgttgaa agatcatccg tataccaatt tatacttaaa aaaattagca
     2881 cacagtactc taacgaaagt aggcgagatc aatgaataaa aaagaaatag cagtagtcgc
     2941 aagttgtaac acaaaattcg taccacattt agctgcatta tttgtctcag tattagataa
     3001 ttgcaacccg tctaaatttg ttcggttcta tgtgattgac gatgatatcg attttgaaag
     3061 taaacagctt ttacgcttct cagtgaaaaa cgcacgaatg aactcagatg tggaattctt
     3121 gaaaatcaat aaagaattct ttacaaatgt ggtaatcagc gatcgtattc cagaaacagc
     3181 ttattatcgt attgcgattc cggaattgtt ccgcggcaca gaagttgaac gtatcttata
     3241 tatggattgc gacatgatcg ctttacaaga tatctctaaa ttatggcgtc ttgacttcgg
     3301 tgattcaatc gtagcagctg ttgaagacgc tgggttccat caacgactgg aaaaaatgaa
     3361 aatttcagct aaatcaatgc gttatttcaa ctcaggtttg atgctgatca atgtgaaaaa
     3421 atggttagat gaaaatatta cgcaaaaagt attagatttc atcgaacata atccagaaaa
     3481 attacgattc catgaccaag atgcattaaa tgctatctta catgaccgtt ggttaccact
     3541 tcatccgcgt tggaatgcac aaggctatat catggcaaaa gcaaaaaaac atccaacagc
     3601 agcaggtgaa cgtgaatacg aagaaactcg caacaatcct tacattatcc atttttcagg
     3661 acatgtgaaa ccgtggagca aggattttga agggccaacg aagaaatatt acgaaaaata
     3721 tgcagggatg acggctttcc gttgtgtagc taaattccct aaatatccaa aatatgcaaa
     3781 agttcaacca aaaacagatg tagttgttaa ataatagaag accacaactg aagaggggga
     3841 gtaggaatgg aaattacaat gtctaaaaat gcagtagaga cgttaattga aaaggttggg
     3901 gaaaatacaa aaatcgcact cgcgcttatc aatgattccg atccatttct aagggacaaa
     3961 ggtgcttttg caaaaggcag cttttttcaa atcattcctt ttgtttcgga atttggagaa
     4021 tatgcgacta aaatcgagca tccattattg gacatctata catctaaatt agagcaaaat
     4081 tatttcggta aacgattgaa catggacttc aacaaacagc ttgacagctt ttcattagaa
     4141 aatgaaatcg cagtactcga ttataatatc aaattaaaga actgcttctt cagctaatag
     4201 tttcgagaaa gtccaaaaag gaggtataca ttgagatact actttgtgga tgaagaaaag
     4261 aatgtaatag aaacagatga aaaaaaatat aattggctcg tacttgatag tgcaaagccc
     4321 gaagagattg agcaagcaat cgaagaattc gagcttcctg atgatatttt cgtaggaacc
     4381 gaatatccgg aagaagtttc tcggatggag cgcctttatg agacgaaact agagcatcca
     4441 ttttctttgg tagtgattaa tctagatcag tcagataatc gtatcgaaaa aaaattaacg
     4501 ccaatatctt ttgtactttc cgataatctc ttgattactt gtatcgatag aaaaaccgaa
     4561 tttgtggatc ggctgctgac aaagcatgga aatcatttaa actcgtttga aaagatcatc
     4621 acgtatacat tattagatat ttacacccat tacgtgaaag aactaagaga gatgaaaaag
     4681 cgaattgacg ctttggataa agcagctcgt aaaacaacgg aaaatgaaga actgtataaa
     4741 caggcagatc ttgaaagaga catcgtatat atcgatcata ccttaagaga tcaacgggaa
     4801 acaatggatc aattatggaa ttctgaaact ttcatcaaac gtttagacga tgaacagcta
     4861 ctctatgatg tccatttgag acagcgacaa acagaaaaaa tgatcgagat ctatcgagat
     4921 ttattagaga gtattggtga tctattcaac gggatgatgg acaataatct aaatcatatc
     4981 atgaaatatc tagattcagc tgcattaatc atttccgttc cggcgttgtt cgcagggatt
     5041 tggggaatga atacaggagg attaccgggt aaaagttctg ctctaggctt tatactcgta
     5101 gtctttggct ctttgatagc agcagtagtt ttaggttacc atttataccg gaaagattat
     5161 acaaattaaa gaatctattt aaaacaggta tcaaacaaag aaatttgttt tggcaataca
     5221 atttaaaaac taggaggaat acagcaatga cagaaccaaa attaatcgac ccgcgaaact
     5281 tgtaccatac ggagaaattt cctaaacaag atcaggatac accagcttta cagcagaata
     5341 tgattcctgt tcctgattgt ggagaagaat cctatgaagg caataatcaa ctagaaaacc
     5401 gccgtgtact aatcactggt ggagattcag ggatcggtcg tgcagcagcg attgcatttg
     5461 ctagagaagg ggcagatatc gctcttcaat tcttcccagg agaagaaaaa gatgcagagg
     5521 aagtagcaaa atacgtgaaa gatgcaggac gtaagattgt cttactacct tttgatcttc
     5581 gtgacgaaca agcacctaaa gaaatcatag ataaagccgt gagtgaactt ggcgggctag
     5641 atacgatggt actaaacgca ggacagcaaa tttcttgtca gtctattgct gagttaccac
     5701 ttgagcaagt caaagacaca tttatgataa acatcatcgc tatgtttgct ttagtgaaag
     5761 aagctgttcc gcatatccca gcaggaggag caatcgtaac aacaacttct gttcaagcat
     5821 tcaatccaag cgaacattta ttagattacg cagcaacaaa agcttcaatc gctaacttta
     5881 cgatcggttt agcaaaacag cttgcaccta aaggaatccg tgtaaatggt gtagcacctg
     5941 gtccaatttg gacaccatta caactagacg aaggacaacc ttctgattcg atccctgaat
     6001 ttggtcagca aagtttacta gaacgtgcag gtcagccagg agaattagca ccagtttatg
     6061 ttttcttagc atcgaataaa gcaagttatg ttacagccca aatttacgga gtaactggtg
     6121 gagaagcgat caacttataa gaagttgatc gtttcacccc tatgaggagg tgcgaaaatg
     6181 aataaaggtg taaaaacgat cggagtgatc ggaacattac ttttgctatc tgtgcttttg
     6241 tttgcgatac tattaagtgc aacctatcca ttgccttttc agctagagaa gtttcgattc
     6301 ttttccatca ctaattcata cgtgcaacaa tatgtatttt ggcttgccgt tgcatttgct
     6361 attttgacaa ttatcacgtt acttgttttc ctgttttatc caaaaacaat cggaaacttt
     6421 ctactgaaac atggtgatgg ggagttgacg ttggataaaa aagcaattga aggaatggtt
     6481 cgttctcact tgcatgaaga tgaattcatc cattcaccaa aagtaaatgt caaagcaacg
     6541 aaaaacaaga tcgccgtcaa tgtaaaaggg gagttgaaac gaacttcagc actcattggt
     6601 aaaacaggaa cattgatgaa agaaatcgaa aatgaagtac aacacatatt gggaacaaaa
     6661 gagccagtga aagtatcggt gaaatacaca ggctttgaag aaccagctaa acgaagcaat
     6721 gaccgcaaac gagtagaata gggggggacc aaaaatgaaa gaactatttt cgacatacaa
     6781 gctccctatc atctttgggg tacttggatt gatattagcc gttttatgga tcaccgtggg
     6841 ctttttcaag accttattga tcttgatcat ggcagcgatc ggcgtcgcta tcggttttta
     6901 tctgaaacaa acgaaaatct tagatcacta ttttaaatct taggaggaaa catccatgaa
     6961 taacaataac aaaaataata acaataaaat gaacaaccaa aaaccagtgt ctgcacatcc
     7021 agacccaacg acaccaaacc aatcaaatgg tgtgaacgat gaaaagaaca aaacagcacc
     7081 agtatctgca catccagatc cagtagtccc taacgttcaa caaggagtag aagagaagaa
     7141 acatgatgca gaaaagcatg cacctcatca tccaggaact ccagaatcta aagttccaga
     7201 acaacatgat gcaatcgatc cttctaaacc tgtggaagag caaaaacatc cttctgacca
     7261 tgcgaaagca caagaaaaac ttagttctga ccacacaaac ggacaaggaa aacttggttc
     7321 tgatcataca aataaccaag gaaagcatga ttctgaacag gaagattcaa tgcgcggtga
     7381 gctgaattat gaagataaag ttgtgcaaaa aatcatcgga atcgcaattg aacaagtaga
     7441 cggtttatta tctgcaaacg gtggtttctt ctctaatgtg gcaggaaaac ttgtgaatac
     7501 agataatgta acggctggga tcgaaacaga agtcggtaaa aaacaagtag cagttgatat
     7561 ggatgtcatt gtggaatatg gcaaagatat cgaaaaaatc tttgaagaaa tgcaagaagt
     7621 aatcggcaaa gaagtaaaaa atatgacaca tttagaagtg atcgaagtca acgctaacgt
     7681 agtggacatc aaaacaaaag aagaatttga aaaagatcaa gaaacggtgc aagacaaagt
     7741 aactgacgca gcgaaaaaaa ccggtgaatt tgcttcgaat caaacagata aagtaaaatc
     7801 agcagctgga agcggcgcac aaaaagtaaa agaaaataca gaaccacggg ttcagtaata
     7861 aggaagtaaa acattaggag gaacgaaaca tgggatttat ttggtcactt atcgtcggag
     7921 gaattatcgg agcaattgca ggagcaatca cgaataaagg ttcttcgatg gggattattg
     7981 caaatgtcgt agcaggttta gttggatcag cacttggtca agcattatta ggttcttggg
     8041 gaccaagtat tgcaggaatg gcaatcgtgc catcattgat tggtgccatc attttagtac
     8101 taatcgtatc attctttgct cgaaaactgt aaaaagactt ttaaaaagag gtcgtgaaaa
     8161 agctgttctt catcaagtaa taagaacagc caaacaaaaa tagcttctca tatttttcat
     8221 ttggttgggc atattatcga agatgcaagc ttttgaacac tgtttatacg gaataagagg
     8281 ctgtgacaag acttttgtca cagcctctta tttatgcggg caaattccca attagatttt
     8341 taaaagtttt tccttaaatg tgtctttatt gatacagaga agcttcttta ataaaacaat
     8401 gcctccctaa attttcttga gataggaaag gtgaacatac aaaataatac gaccactttg
     8461 aaaacccaga tgatccacaa atggatcgta tgatactgat gaagtaaaaa gcaatggatc
     8521 agaaactcaa agagattgaa aaagacaagg aagaaataat atctcttttt tactcttgtc
     8581 tgttttagcc aataatagct cgtaaagtaa tctagacaaa caaatagaag taaacaaagt
     8641 ttcgtaagag cagcaatcgt atcatgttgc tctgctagac gattgacgag caggaaaaat
     8701 gttggtaatg gaatatagat cacgagtaat gaaagaaaaa agtaaacaat aaatggcata
     8761 ctattcctcc taattgtgtg agttcgccct cattttaaaa taacgatatt tgtttgtgaa
     8821 gctttttcgt gtcgtaacaa atgttatcta atgtattttt aaataaataa tcattattat
     8881 caaaataatg
//
LOCUS       DAZAID010000110         8750 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000110 DAZAID010000000
VERSION     DAZAID010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.113"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(348..2729)
                     /locus_tag="ACG9BE_002507"
     CDS             complement(348..2729)
                     /locus_tag="ACG9BE_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFN3033218.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2719..3366)
                     /gene="recU"
                     /locus_tag="ACG9BE_002508"
     CDS             complement(2719..3366)
                     /gene="recU"
                     /locus_tag="ACG9BE_002508"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFN3033219.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3430..3969
                     /locus_tag="ACG9BE_002509"
     CDS             3430..3969
                     /locus_tag="ACG9BE_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFN3033220.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4059..4478
                     /gene="gpsB"
                     /locus_tag="ACG9BE_002510"
     CDS             4059..4478
                     /gene="gpsB"
                     /locus_tag="ACG9BE_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFN3033221.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4500..4867
                     /gene="rnpB"
                     /locus_tag="ACG9BE_002511"
     ncRNA           4500..4867
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG9BE_002511"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5076..6242
                     /locus_tag="ACG9BE_002512"
     CDS             5076..6242
                     /locus_tag="ACG9BE_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFN3033222.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6288..7784
                     /locus_tag="ACG9BE_002513"
     CDS             6288..7784
                     /locus_tag="ACG9BE_002513"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFN3033223.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7865..8548
                     /locus_tag="ACG9BE_002514"
     CDS             7865..8548
                     /locus_tag="ACG9BE_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN3033224.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8557..>8750
                     /locus_tag="ACG9BE_002515"
     CDS             8557..>8750
                     /locus_tag="ACG9BE_002515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033225.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2760 a         1582 c         1848 g         2560 t
ORIGIN      
        1 tatcagataa ctcaaaatga agccttcttt caaaaaatta ttcaaaacca tatattgtat
       61 tttttatttg ataataacaa tgggattatt aagaagtggc actaaataaa agactagtgt
      121 gtatttagcc gatttaatta taaacaagta aatggataaa gtatatgtaa tgataaaaga
      181 aaaatggatt ttctaatttt taagctaaat gttagatcac acatgtatat gctggatagt
      241 cttataaaaa atcatgtaat acagcttggt acatgatttt tatttgtagc tgttttttac
      301 atagacacaa aaaagaaacc acgcttgtag acgtggtttc ttttttacta tctgtttcca
      361 gaagctgagg tgctgctgga tggtgtatgg tcatttgcag catccgctcc gctagttgct
      421 ggctcactgg aagaagcagg aggagcagat gaagacggtg tcgagctttc ttcggatgaa
      481 gaagaaggaa ccgttgttga agacgctggc tcgcttgttt ctgctgtcgt gctttctttt
      541 gaggactcag cagttgattc gctttgacta gtagagctag agctttgagt cgttgtctct
      601 gttgttgagc ttccaggagt ctggacatag ctggaagatg gaatagttgt tgatggtgta
      661 attgcattcg atctagctgt atattgatct ttgtagtaca gttcaccgcc cacacgaatt
      721 aatccgcttg gcatctccca gtctgtattt gtcacgttag ctgaaacata ctgcataagc
      781 tcgcgataaa catcagaggc tacgtgtgac gattctgaag tgacaggcgt cattttttta
      841 ttgtaacctg tccacacgga aatcgaataa tttggcgtat agcccgcaaa taggatatct
      901 ggatacaccc cagaagaaat accaagtttt gcatactcgt catctgtgta attggatgtt
      961 ccggtcttac cggcttgata taacccagcg atttgcgcat tcgtaccagt tccttcagtg
     1021 atcgtatctt ttaaaatgtc tgtgatcata taagctgttt ctggagacat ggctgttttt
     1081 ccatctggct cataagtttc ttctgttccg tcttggaaga cgattttatt aacatattga
     1141 ggtttgtaat aagtccctcc attggcgaaa gccgcatagg ctgctgccat ttttaaggaa
     1201 gaagctccgt attttgtccc atcttgttct tccgtattac tagagatcgc attcgattga
     1261 tggatcgtac tgtattcgat cccaagattt tttaagaaag aagccacttt atctgaaccg
     1321 acttcattga ataatttgac tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa
     1381 gtaatggtgc ccatatattg attatcccag tttcctacag gagtcgaagt cccttcatag
     1441 ttatacggtg cgtcagtaat agtttttcca gtagaatatt tcaaatattc aaaagctggt
     1501 ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac gggaagtatt cactgctaag
     1561 ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc caatctgggc ttttactttc
     1621 ccggtattgg tgtcgataag ggtggaggca acttgcatct cgtcgtctgg gtaagagaca
     1681 tactgatccg tgttgacgat atcatatagt ttcttttggg cgtccagatc caaattcgta
     1741 taaatctcta aaccgtctgt gtagacattc ttatctgttt tttcttgtac ttcattgatg
     1801 acttctttga cataattatc gacgatctta gtattgtcat cggattgagt tagttcttgc
     1861 agaccatccg ttaccggtac attaacagct tgatcatatt ctgtttgaga gattttttcg
     1921 ttttgaagca tcgtatacaa aacagtatct cgtcttttct tcgcctgatc tggataaaca
     1981 taaggatcat aggcggaagg ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa
     2041 gaaagttctg ataatttctt accaaagtac atctcagaag ctgtctccat cccgtacaag
     2101 ccgtttgaca tatacacttt atttacgtag taggtcaaga tctcttgttt cgattttttt
     2161 tgttccaatc ttacagccat ccacgcttct tgggccttgc gtttcagagt ctgatcctcg
     2221 gcactggtgg agaagaagga aagtttgatc aattgctgtg tcaaagtact tccgccttgc
     2281 agtccgccag aggtaaagtt agagagggca gaaccgatga tccgaatagg gtcaaccccg
     2341 atgtgtttat aaaaacggcg atcttctacc gacacgatcg catcttctaa tgttttcggt
     2401 aattcattcg cagaaatttt ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca
     2461 tcttgtgtat atagtttgga agatacggta gcgtctaatt ttttatccgt caattcgggc
     2521 gcatcttttg cgtaatacca gaaaagtcct acacctgcta aaaagaggat acagaaaaat
     2581 gagagtattc ctaaaaggat cttgataaat aatccttttt tatgtgttcc agaggacttt
     2641 ccagaacctt tgccagaatt cttcttcaca ctctttttag gagtaggctg tttctgtcgt
     2701 ctagagcttc tagtttgttc atttgccatt cttgctttct ccttcaatag ataatgttga
     2761 taaatagaag tcaaccgcgt ccaaataagg aatccgcgga gaaataccag ttttgatctc
     2821 tattcctagt tcttcgatca aagataaagg tagtgatttg cgtccttttt ccttttgggc
     2881 gtcccagtaa gcaatcaaat gaacactatt caaataaaaa cagcgcttca aagacgaaaa
     2941 ccataacaag acaaagcaga ccccattttg ttcgaggcat gctttcatat gttcgatttg
     3001 atgggcatgg aagtttttaa agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa
     3061 gtccaagtaa aagcctcgat agacgccatt atagtctgta gttgaggctt gggtaaagta
     3121 ggcttccttg atcacagcag cacttctctt aggatagtct actttcacga tttggatagg
     3181 ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga taatatttat tactttcatt
     3241 gattgcttct tcaaagcgca tcccccgatt gccgaattca gtgatctgat ttcttttttc
     3301 ttgaattttt tcgacgggag aggcattctt ataataaggt tggccatttg gatagcgcaa
     3361 taccatcttt aatcacactc ctaggccaca ttatagcaaa aaagcggata gaaagaaaag
     3421 gaatctgaga tggataagtc aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc
     3481 ggtgttttta aagaaaatga tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa
     3541 ctccatcagt tagcagaaga agggctggaa tgggtacttg tcagtgggaa tttgggcgtc
     3601 gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag aatatccgga actatctctt
     3661 ggtattttgt atcctttttc tgattttggt aataactgga atgaagggaa ccaagaaaaa
     3721 aaacaattag cagaacaatt agcagattat gtcgaagctg ttagccatca accctaccaa
     3781 tcacctagtc agctaaagaa tcacactcgt tttatacttg agcatacagt gggctgtctg
     3841 ctgatctatg atgaagaatt ccctggaaaa acgaagtatt tttttgaaga tgcacagaat
     3901 taccaagcag atcatcccta cgaaattcgg ctgatcagta tggacgatct tcaaaatttt
     3961 agtgaataat cgatattgtg tttgatttat caactttttt cctatacaat gaaaaaggta
     4021 gaaatgttat tacgtaaatg aaaactagag gtgtaaatat ggcgaatttg gtttatagtc
     4081 ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg cggctatgat cccgttgaag
     4141 ttgatgaatt cttagacaat atcatcaaag attatgaaac ttacagcaaa gaactccttg
     4201 ctttacaaga agaaaacgat cgattgagtg caaaagtagc tcaattatct aaaactcaag
     4261 gagcagctca aacacgcgtg caacaaacag aggcaccaaa gagcgcagca gtgaccaatt
     4321 ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc
     4381 aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa taactacaca aatgcagaca
     4441 caagcttaga tgataatgaa aaaacacgtc aattttaatt tttgattaat gaatttgcgg
     4501 ttttcgggta atcgcggtct gacttgatca ggctgaggaa agtccatgct cgcacaggct
     4561 gtgatgcctg tagtgttcgt gcttagcgaa accataagct aaggtactct ttggagtaac
     4621 ggcaggaaaa caagctaagg cttcggctat gcttaagtat ccttgaaagt gccacagtga
     4681 cgaagcggct ggggaaaccc atccggtgga acgcggtaaa cccctcgagc gagcaaccca
     4741 aacaatggta ggggcgcttt ttgtctggaa gaagaacagg acaaaaaggc agtataactg
     4801 cagatagatg gttaccgctt tgttttttct ccctggaaaa gcaaagtaca gaacatggct
     4861 tatagaaaac cgcaaacatc agggaattag gtcgtgacaa aagtaatgcg ttataagaaa
     4921 caagaaaaac atttgaaagc tatttttcaa tacttcagat gaatcgttct tattacaaaa
     4981 caaacttttg tatcaccggt tattcggaat ttgagtaaaa gtgtggggta ggactgatgt
     5041 cccacctcct ttttttataa ataaggagat tgtgtatgga aaaaaacagg aagttcaacc
     5101 tcgtcgcaac tgcggcaagc gggctagaag cactggttgg taaagaatta agagatcttg
     5161 gaatcgaatg ccaagttgag aacggacgtg cacgttttga aggaacaatg gaaacgatcg
     5221 ctacagctaa tttatggtta cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg
     5281 atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc attgccttgg gaagattttc
     5341 ttccactaga tgcagctttt cctgtagctg gaaaatcgat caagtcgaaa ctatatagca
     5401 cacctgattg ccaagcgatc acaaaaaaag caatcgttga acgtctccgt acctattatc
     5461 accgtccagc ttctgtacca ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt
     5521 tgctgaaaga ccatgtgatg gtaactctgg atacgacagg accaagttta ttcaaacgtg
     5581 gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa catggcagca gcacttatta
     5641 tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc
     5701 tttgtattga agcagcctta ataggccata atatcgcacc aggattcaat cgtgagtttg
     5761 cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga aaatgttcga tcagcagccg
     5821 aagaaaaagc agattatgat attgaattag atattactgg ttcagatatc aatggacgga
     5881 tgattgaaat tgccaaagcc aatgcagaag aaatcggatt aggctcttcg attaccttta
     5941 agcaacaagc agtcaaagac ttcaaaacgg ataaagaata cggggtcatc gtggctaatc
     6001 cgccttacgg agaacgtcta ggagaagaag aaactgttcg gaaattatat aaagaaatgg
     6061 gcgatgtatt ccgtccatta aaaacatgga gtaaatatat cttgactagc gacttagcgt
     6121 ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc
     6181 gtacggatct attccagtat tggggaactc gtccgcctag aaaaaaagag aaagacgaat
     6241 aatcttacta attgattcat tcaattggat gtttggagga atagggaatg aaagaaaaag
     6301 agtttttaca agaagtaaaa gaaatcaatc ttctacaaca agctttagga atcctcgatt
     6361 gggatacaca aacaggtatg cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct
     6421 atctttacag catttatttt tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct
     6481 atttttctga acatccggaa gaattgtcgg aagttggaac tgccgttttt gccaaagtaa
     6541 aagaagagta tgatttggaa catcgcgtac cggaagatct tatgagaaaa tatacagctg
     6601 ccgtttcttc agcacacgga caatggcaaa aagctagaga aacacaacaa ttttctgatt
     6661 ttcaacacgc attgaatcaa aatattgaat taacaaaaca gttgatacct tactggagaa
     6721 aagatgaaaa gacgaattat gatgttttgc tgaatcaata cgaacccggg atgactgtcg
     6781 aaatccttga taaggtattt gatcagctta aagaaggaat tctttcgatc cgccgtacgc
     6841 tccaagaaaa agggactgaa cctgaaacag actttttaaa tcggagaatg acgaaaaaac
     6901 aacagaagag atttgtagtg aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc
     6961 gtttagatga cactgtccac ccatttatga ctggaattaa tccgaatgat gcacgaatca
     7021 cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt tggagtgatc cacgaggctg
     7081 gtcatggtat gtatgaacaa gacatcgatg ataaatatgc gtatacaccg gtttatgaag
     7141 gtacatcaat gggtatccat gaatcacaat cgttgtttaa cgagatcatt attggaagta
     7201 gtcgcagctt ctggaaaaag caatatcctt tctttcaaga atgtgcagaa ggaacattcg
     7261 atgatattcc ttttgacaaa ttttatcgtt cattgaacca tacaaaatca agtttggttc
     7321 gtatcgaggc agacagtttg acttaccctc tccatatcat cattcgttat gaaatcgaaa
     7381 aaatgatttt caatgaaggg gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat
     7441 atgaagagta tctgggaata cgaccaacaa atgatgtgga aggcatccta caagacgttc
     7501 attggtcagg tgcaagcttt ggttattttc cctcttatgc attagggtac atgtatgcgg
     7561 cacaattact tcattctttg cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg
     7621 attattctcc aatcaaaaac tggatgtcag agcatatcca tcaatatggt gcatctagaa
     7681 aaccaaatca attaatcatg gatgcaacac atgaaccgct taatccgcaa tacctgatcg
     7741 actatatgaa agacctgtat tttaatgtat atcaggtaaa ataaaataca atttacaaag
     7801 atagctgaaa ttcatctaat ctatagatgt tttcagctat ttacttaaga aagaagtgga
     7861 acgaatggat aaattacaag tgatcgctga ttatagtatg gaccaattag cgcatgatca
     7921 aacaggacat gggagcgatc ataccaaaag agttgtcaag ttagctgaaa aaattttgga
     7981 gacggaaccg caggcagacc gctttgtgac gcttgcagca gcttacctac atgatacgat
     8041 tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa cagcttcgtg tattccttcg
     8101 ctctttgcca atagtagagg aacagatctc gatgattttt tccattatcg aaaatatgtc
     8161 cttttctaaa aatctctctg aagcggccga actatctctg gaaggtaaga tcgttcaaga
     8221 cgctgatcgt atcgaagcat taggagcaat cggcattttg cgtactgctt atttcggcgg
     8281 tggacatggg cacccgattt ttgatcctga actgtatccg caaacattca aggacaaaga
     8341 aaattaccga aaaggcacga cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc
     8401 tgataaaatg aatactgatt acggatatga agaagccaaa cgaagagaaa ccttcatgcg
     8461 tgaattctta gaagaatttt ttctggaatg gtctattgac gatgaaaatt ttactccaga
     8521 caattggatc aaaaataaca aaaaatgatg gaggttgtga cagaagtggt cagctccaag
     8581 atatttcgga tcattttccg taaaagttac ttctggaaca ctgtttacca agaggtcgtg
     8641 agcctgccgt tcagctccga gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg
     8701 atgaatattg taacttgtga ttcggaattg gcaggagaac accgtttatt
//
LOCUS       DAZAID010000111         8577 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000111 DAZAID010000000
VERSION     DAZAID010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.114"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..679)
                     /locus_tag="ACG9BE_002516"
     CDS             complement(50..679)
                     /locus_tag="ACG9BE_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFN3033226.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(672..1790)
                     /locus_tag="ACG9BE_002517"
     CDS             complement(672..1790)
                     /locus_tag="ACG9BE_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFN3033227.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(1792..2424)
                     /locus_tag="ACG9BE_002518"
     CDS             complement(1792..2424)
                     /locus_tag="ACG9BE_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFN3033228.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(2435..4252)
                     /locus_tag="ACG9BE_002519"
     CDS             complement(2435..4252)
                     /locus_tag="ACG9BE_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFN3033229.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(4265..5029)
                     /locus_tag="ACG9BE_002520"
     CDS             complement(4265..5029)
                     /locus_tag="ACG9BE_002520"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFN3033230.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5057..5755)
                     /locus_tag="ACG9BE_002521"
     CDS             complement(5057..5755)
                     /locus_tag="ACG9BE_002521"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFN3033231.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(5767..6546)
                     /locus_tag="ACG9BE_002522"
     CDS             complement(5767..6546)
                     /locus_tag="ACG9BE_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFN3033232.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6562..7506)
                     /locus_tag="ACG9BE_002523"
     CDS             complement(6562..7506)
                     /locus_tag="ACG9BE_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN3033233.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7552..8331)
                     /locus_tag="ACG9BE_002524"
     CDS             complement(7552..8331)
                     /locus_tag="ACG9BE_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033234.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2322 a         1775 c         1453 g         3027 t
ORIGIN      
        1 tcgactgata taatcaaaag gatgaataag cagccacttt tttttattct cactcatcta
       61 tcttcgcacc ctcaaatttc tcagtagtcc cacttgctac tgatgttatt ccatcacgga
      121 caataacttt ctttagcgtt aaaaaaaata tttttatgtc tttcatcatt gaaatgttct
      181 ccacatattc aatatctttt tcaaattttt ctttccaact aattgcatta cgaccactga
      241 cttgagctaa tccggttaaa cctgggcgca caaaatgtct ttttcgttgt tcattactgt
      301 atagtgaaag atactcaact aacagaggac gcggtccaat aatactcata tctcctttta
      361 aaatattcca taattctggt agttcatcaa gactagtcga acgcaatatt ttaccaaatt
      421 gagtcaatct gatatcgtct gataataaat taccttcttc atctctttga tctgtcattg
      481 ttcgaaattt atacatcaga aaaatctttt catgttttcc aggtcgttgt tgtttaaaga
      541 tcactggcga tccaagcttt attcgaacta ggattgcaac aaacgtcatc agtggccata
      601 acattattaa acctaaaatt gaaaaaagaa tatctatcaa acgtttaaag aacttttcgt
      661 agatacctct attagacatt ttgcatcact cgcttaattg tattagttac tcgatacaaa
      721 tcatcatcgg tcattttaga atctgaaggt aaacagatac ctttttcaaa caatgtctga
      781 gcaacatctg taccaatata ctcatactta tcgaaaactg gctgtaaatg cattggtttc
      841 catatcgggc gggcttctat attattttta tctaactcat caataagatg attcgtattt
      901 attcccttta ctgttatgac acttaaccaa taatttggtt cattccaatt attttctggc
      961 atgaattgta tatttgacaa tttagacaat tcttttttat aaaaactatg aatatatcgt
     1021 tttttctcaa ttctttcatt taaaactttc aattgtcctc gaccgatacc tgcaacgata
     1081 ttgctcattc gataattata gccaatttca ttgtgttgat aatggcgtgc cttctcacga
     1141 gattgcgtag cccaaaacct cactttttcc accttttctt tgtcattaga cacaagcatt
     1201 ccgccacctg aagtcgtgat tatcttattt ccattaaaag aataaattcc atagtcgccg
     1261 aatgtaccag tatattttcc tttatatgtt gttcctaatg attctgcagc atcttcaatt
     1321 aaaacaacat tatgttcttt gcatatttta gtaatttcat ctagttcagc tgataaaccg
     1381 tacaaatgca ctactaatac agcttttgcg ttaggatatt tttctagcgc tgttcgcaaa
     1441 tgttcagggt tcatattcca agtttcgtaa tcactatcaa caaatactgg aatagctcct
     1501 tgatagatga ttggattggc agtagctgag aacgtcaaag actgacaaat cactatatca
     1561 tctttaccga ccccagccgc ttttaaagct agatgtatag ctgctgttcc agaagatagt
     1621 gcagctgcat gactagcacc gacatattct gctaattcct cttcaaaacc attgacattc
     1681 atacccaaag gagccaccca atttgtatca aatgcttctt ttacatactc ttgttcatat
     1741 ccttcatcac tcatatgagg agatgctaat aatattcgtt tattttccaa cttaatccca
     1801 tctttctatg actttggtag gtacgccaac atttttagtc ttattattaa tattttttat
     1861 tactacactt cccgcaccta taatgttcat atttccaatt cttagatttt gtattatttt
     1921 agtacctact ccaatttcat ttttatctcc taatagaaca tgtcctgaaa tatttacact
     1981 tggaaaaatt gaattataat taccgatttg agtatcatgt ccgatagtag agccgatatt
     2041 gatcatattg aaatttccta aaactacgtc agcagtgtaa gtcgtatagg gcatcaaaat
     2101 attgccaatt cctaactgaa catttatgcc cactagtgca cttgatgcaa ctaaattagg
     2161 gaaaatcaat ttattatttt tatttatctt ttgatatatt ttctctttga tatctggcga
     2221 agcgattccc agaaaaatag cagtttcttc ttctaattct aataacgagt ctattgttcc
     2281 caatacagtt tttcccacta attcttgatt ttgcattttt aacgaatcat cgacaaaacc
     2341 aatcaattca tatctagaga aactttctag tagaaaagca atctctttgc caaatccacc
     2401 agctcctaca ataattgctt tcactttact ccccctactt attcgaagca ttcgcaaaag
     2461 tgacaatgtc tttagccaat tgttcatcgt cttgcggcag gctcttgaca aattccatca
     2521 ctgtttgcat ggaataacct ttgatgtttc ccacaaagat cttctcgtaa actgcttcat
     2581 cgtttctttc cttatctaac aatagttctt catatagttt ttcccctgga cggatacctg
     2641 tttcaatgat ttcgatctcg tcttcgctat atccgctcaa tcggatcatg ttttttgcta
     2701 gatctacgat tttcactggt tcactcatat ctaaaataaa gatttcgccg ccttttgcta
     2761 acgccccaga ttgaataact aaacgacttg cttcaggaat cgtcatgaaa tatcgggtca
     2821 tacgaaagtc tgttacagtg actggccctc cttgagcaat ttgttcccgg aatactggaa
     2881 tcacactacc gcgagaacca aggacattcc caaatctgac agctgagaat ttcgtacatc
     2941 cttcttcatt caacccagtc acgatcattt cagcgattcg ttttgttgaa cccatgacgt
     3001 ttggcggatt gttggcttta tctgtcgaga ccatgacgaa gttttttact ttcgctgctt
     3061 tggctgcttc tgctacattt tttgtaccga aaatattgtt tttgactgct tcttttgggt
     3121 tatattccat caatggtaca tgcttatgag cggctgcatg gtagacgata tctgggtggt
     3181 attgttccat gatctcattg attttcttgc gatcttggat atccgcaatg atcggcacga
     3241 tttctgtagg acacttttga tgatgcgtgc gtaattcacg gtcaatcaag taaatcgagt
     3301 tctccccatg acctaaaagt aatagttttg cagggtcaaa ttggatgatt tgccggcaga
     3361 tttctgaacc gattgatcct cctgcaccgg tgacaagaat gactttgttt gtgatctggt
     3421 ctttgatgga ttcgatatcc aactctacct catctcttcc cagtaattcc actacgtcga
     3481 tggtttttaa ttttgatacg ctgattttcc ctgaggcaag ttcttccata gaaggcatcg
     3541 tattgacttt cacatgagca gattcgacta actcgaagat tcttcgtaat ttttttcttg
     3601 ataaagaagg gatagcgatc gttaccattt ggatgtcata tttttcgatc aattccggta
     3661 tatcttttaa tgaccccagt actttttttc ctgaaagata ggtatttctt ttattcggat
     3721 catcatccac gaatccaatg acatggatat cttgagccgt tttagaacca agaaagctgt
     3781 tgtataaaat acgtccacct tcgccggccc caacgatcaa ggtattttta gcggaatctg
     3841 ttgatagata cctcatattt tttgtttcga tatagatacg ccagatcaat cggctgccaa
     3901 tgatcagtaa tagcgaaagt aggtatgcaa agatcactaa acgtaaactg tattgtttat
     3961 ttatgaaaag caatatgatg atacttgagg ccgcacttgc agataacgaa ccaaaaatcg
     4021 caatgatctc tcttaaattt gtataacgat tgattcgagt aaaaactttg aataaactac
     4081 catagaataa ataaaaacca atcgacaaac cgatagagat taaaataagg tccattggta
     4141 tagctacaaa cggcttcatg aatttagctg ctgcaatatt cgcagcaatc aacaacaagc
     4201 tgtccacaaa taccaatgtg gcaatttttc ttcttcttgt taatacaaac accctacatt
     4261 ccccctaaaa taaaccaaac ttcttctttt ttacctcttc atagactgga taactgatct
     4321 catccccgtt gatcaggtcc tttgcgactt gtttcatctg tgctactttt tccttaccaa
     4381 agtccttggc gatttgctca taacattctt ttaagtaaaa ggtacgttta gagaccccat
     4441 gggcatcgga agcagccatt tgcacgaggt tatgtttgac catcattttg gctgttttct
     4501 ggatgtcttt tccaaataca ccgacataac ttggtgcggt gagttgagcc aagcatccca
     4561 tatctaggaa tggcagcaaa tgatttgggt cttctcgaaa atgcgcgttg cgttctggat
     4621 ggacgatcac tggtgtcttt cctagttgta acaactcaaa aaagagccat tctgcatata
     4681 acggcacttc cagtgtcgga aattcgatta gaagatatgt atcatctaaa tctgtaaata
     4741 aaatttcatc tcggtggatg tcttcaatca gtgttccggt aatacgtact tcttgtcctt
     4801 ccaataacgt caaaggcaat tgtcttttct ctaattctgc ttgtaacgaa gcgactagag
     4861 aaatgacttg tgatttttca ttgctgtatt ttccattatt gtgatggggt gtgcataaaa
     4921 tatgtgtgat cccttgttga atcgcttttt cagccatggc gatactggca tctatgtttt
     4981 ctgctccgtc atcgattcct ggcaggatat ggcaatgtag atcgatcatt tctcttgtct
     5041 cctctctttt tatttcttaa ttcccgtagt aatagccagc atctctgctg tgttctgctc
     5101 cattgtatac aacaccaagg actcgcgctt gtaccatatc taacagttct tttgctttag
     5161 tcaatgattc ttttcgtgac gtattttccc gtacgaccaa taaggtgcca tccgtttttg
     5221 aagacatgat ctgtgcatct gttacggcaa cgactggtgg catgtcgaaa atcactacat
     5281 caaatagttg tcttgcttct gctaaaatct gatccattct tggcgaactt agtagttctg
     5341 atggatttgg tggtttaggt ccacttggca gaatcgacaa attgtcgact ggtgttcgtt
     5401 ggatctcatc tgctacactt cctgatgaac taagaacagt acttaatccg cttgcgttgt
     5461 tcaacttgaa cgttttatag atgacgggct tacgtaagtc tgcatcgact agcagtaccc
     5521 gttgaccaga tttggcaaaa acaacggcaa tgtttgctgc agtggtggat tttccttctc
     5581 caggacctga tgaagtcacg acaatcgttt tgatttgctg accagctgcg gaggcaaact
     5641 ggatattcgt ccggatcgta cgatattgtt cggcgattgg tgaagatggg tttgttaatg
     5701 tgatcaaact gactgcatga gtttgagttg ctttttgttt ctgtgttcgt gccatttttc
     5761 ttcctcctag actcttgagc ggcttctgcg tgatggggct gttttatcgt ttgtagcttt
     5821 tggtcgtgca ggagctgatc cttttttacg gataccagcg ttcaattctt ttgctgacat
     5881 ttctggaact gttcctaaga ttgggaatcc taatgtatcc gtcacatatt tttcatcttt
     5941 cactgtccga tcaagtaatt cgaataagaa tgccagacct acgcctacca tcattcctaa
     6001 cactaagcca atcactagat tcaatttatt atttggcgaa acaggtgtcg tattggcaac
     6061 tgcacctgaa ataatcgaga ttttatcgac attcatcacg tctttggcgt tttcttggaa
     6121 aacttgtgaa gttgtattag cgatctgttg agcagtcact gcattcgtgc ttattgcctg
     6181 gatcgagaac atttgtgaat tttgtgactg attgacggag atcgcatctt tgatttgtcc
     6241 agcagtcatt ttcacattgt cttctgtttc taaacgatct ttgacttcat ttagtactaa
     6301 atcactgacg atcatatctt tatacgtatt gatcatttgc aggttcgtat tgacatcatt
     6361 gacatttgct gtgtctgatt gtggcagtgt cacgatcaat tgtgcttgtg aactgtattt
     6421 tggtgtcaag atgaaaaatg ttacgattcc tgagatggcc aagccagcaa acattgagat
     6481 gatgattgtt accaagtgtt tgcgtaatgt ttcaaaaatt tctttcaagc tgattgtctc
     6541 ttccattttt tctccacttt cctatttatt ctctagattc atttgatctt ttaattcttt
     6601 tttcacacga tcaagttctt gatcgctgac tttttgatag gagatattat cttgcatgaa
     6661 gccttctcct tgcagctgat cttgcttgat cgtaccaaat gcctcccgat aatctaaagc
     6721 gatttttttc atttcatcaa agctcatgtc tgttttcata ttcgactcta gagcattcaa
     6781 aacggattga tagttgctga caccgtttaa actcaatact ttttttgcta caccttctac
     6841 gatctttctt tgacgctctt gtctaccata gtcaccattt ggatcttcat aacgcatacg
     6901 agaataagat aatgcctgat ccccgttcaa tgtaatttta ccgattggaa agtcatagcc
     6961 atcttgtgag aaagtgaggt cattattgac ttcgatacca ccaactgcat caacgagttc
     7021 ctttagccct ttcatattga tagagacata gtgatcgatt gggatgtcta catatttttg
     7081 tagggtatcc atcgccatgg cagctccgcc gaatgcgtaa gcgtggttga tcttgtcttt
     7141 tgtgtcacga cctacgatgt cgacatacgt atctcgagga atgctgacga tcgttgtctg
     7201 tttgtcttca ggactgactg tggcaaccat catcgtatct gatcttcctt gatcgacccg
     7261 acctaaatct ccagtatcaa tgcccatcaa tagaacagag aaagattctt gtttgtccag
     7321 atcaactggt ttcgttcgtt ctccttgggt tcgttccact gattcgtaag ttttctcaat
     7381 agaatgtgag agatccagat ataattttgc tcctactcct acaattgcta atactaatac
     7441 gataagaact gctaaaataa tcagaaatat ttttttccct cttgattttc ttcttcgtct
     7501 tgccatagtt tcctccgttt gctaaccttg cttgaatcaa cgttttaact gtcaataact
     7561 tatattgcta tagcccattt gctgagcaac agtttgtgct tttgttgcgt atgctgcgct
     7621 tattttttca ttccggatct gctctatttg actgagcgcc gtcaaatatt gatcgtaagt
     7681 agcggtggca gtcacttgac catccttcaa catcgtatcg aataactgat ctagttgcgt
     7741 gacttgcgcg atctgagtat cagcgaatcc tagatattgc ataacaaccg ttttccaagg
     7801 actatcttta aaaaagctca tattttcacg tacacgagcg acatctgcac ttgtcacctt
     7861 ttctttgatg acgacatcgt ctacagcttg ctgccagttt gatacatttt tagtaaataa
     7921 atcgttgaca gctgtctgga tcttcagttt tgattctgca ctatctaact gtgtcacgat
     7981 tgctttcttc tcatcttgga ttgcaacagc ttctttaggc aattctgatt ctttgatgtc
     8041 gtaatcatct gctgaaagtt tgacagagga tagcttattt gacaaacttg tgattgtttc
     8101 ttctgttatt tcagggttta gaaaattttt ttcttttgta ctatacagct tgtctacttc
     8161 ttttgataaa gaagtcaagg tgtctttttg attggtgatg gcagattctg cataattgat
     8221 ccgttgctga tgttgccgct gattattgat atggtaacca aacaaacaga tcgcaatagc
     8281 acctgcacct accgccacag cagtcaatga cttgacgaat ggcttttcca tagattcact
     8341 cctaagtatt gatgatagat aaattaagat ataattcaca tacataaaaa tgacagcttt
     8401 ttattttaaa aaacataatt aaattaatat attcacagaa atatttgtaa acaagttttc
     8461 cctatataat cataccacaa attgttttct tgactagggc aatttacgat ttaaaagtaa
     8521 ataaaacatt aataagataa caattaatcg atttcatttt tatttcctta attttca
//
LOCUS       DAZAID010000112         8273 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000112 DAZAID010000000
VERSION     DAZAID010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.115"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG9BE_002525"
     CDS             476..1339
                     /locus_tag="ACG9BE_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN3033235.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG9BE_002526"
     CDS             complement(1468..3090)
                     /locus_tag="ACG9BE_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN3033236.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG9BE_002527"
     CDS             3339..3860
                     /locus_tag="ACG9BE_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFN3033237.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG9BE_002528"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG9BE_002528"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFN3033238.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG9BE_002529"
     CDS             5941..6759
                     /locus_tag="ACG9BE_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFN3033239.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG9BE_002530"
     CDS             complement(6862..7059)
                     /locus_tag="ACG9BE_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033240.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG9BE_002531"
     CDS             7228..7806
                     /locus_tag="ACG9BE_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFN3033241.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG9BE_002532"
     CDS             7901..8038
                     /locus_tag="ACG9BE_002532"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033242.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2489 a         1485 c         1632 g         2667 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ata
//
LOCUS       DAZAID010000113         8023 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000113 DAZAID010000000
VERSION     DAZAID010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.116"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..388
                     /locus_tag="ACG9BE_002533"
     CDS             <1..388
                     /locus_tag="ACG9BE_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN3033243.1"
                     /translation="NAKLEEEARKVSNYNKKMAAKYVNEEEFCFSKTKIYKRNPFVSE
                     YVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQGGKDVIENCIALCPNCHARIH
                     ELELPDDKEKLLKIVEKRENSFSKIP"
     gene            731..1054
                     /gene="relB"
                     /locus_tag="ACG9BE_002534"
     CDS             731..1054
                     /gene="relB"
                     /locus_tag="ACG9BE_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFN3033244.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            1374..1601
                     /locus_tag="ACG9BE_002535"
     CDS             1374..1601
                     /locus_tag="ACG9BE_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFN3033245.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1617..2783
                     /gene="hemW"
                     /locus_tag="ACG9BE_002536"
     CDS             1617..2783
                     /gene="hemW"
                     /locus_tag="ACG9BE_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFN3033246.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3018..4061
                     /gene="hrcA"
                     /locus_tag="ACG9BE_002537"
     CDS             3018..4061
                     /gene="hrcA"
                     /locus_tag="ACG9BE_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFN3033247.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4155..4718
                     /gene="grpE"
                     /locus_tag="ACG9BE_002538"
     CDS             4155..4718
                     /gene="grpE"
                     /locus_tag="ACG9BE_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFN3033248.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4769..6598
                     /gene="dnaK"
                     /locus_tag="ACG9BE_002539"
     CDS             4769..6598
                     /gene="dnaK"
                     /locus_tag="ACG9BE_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFN3033249.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6749..7915
                     /gene="dnaJ"
                     /locus_tag="ACG9BE_002540"
     CDS             6749..7915
                     /gene="dnaJ"
                     /locus_tag="ACG9BE_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFN3033250.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         2767 a         1345 c         1753 g         2158 t
ORIGIN      
        1 taatgctaaa ttagaggaag aagcaagaaa agttagtaat tataataaaa aaatggctgc
       61 aaaatatgtt aacgaagagg agttttgctt ctcaaaaaca aaaatatata aacgtaatcc
      121 cttcgtatct gaatatgtta aacgacttgc aaaaggtatt tgtcaacttt gccaagaaaa
      181 agggccattt ataaaagacg gtgtacctta tttacattgt catcatatag aatatttgtc
      241 gcaaggtgga aaagatgtga ttgaaaactg tatcgcgtta tgtcccaatt gtcatgctag
      301 aattcatgaa cttgagttac ctgacgataa agaaaaattg ctgaagatcg ttgaaaaaag
      361 agaaaatagc ttttctaaaa taccgtaatt tatttttata ggacaaataa gctaatgtga
      421 gctatgcctt ctatataaga ctaagttcta ttataagtaa cgaagtaatt ctcgttcctt
      481 cctgtagtaa tattttttaa ataacctata ggcttcttca aaagtacatc ctccagtaaa
      541 ttctaagtac tggaggattc tttttctagt gttgctttac tcatgaaaaa gtagttggct
      601 acttcatcaa aatttctgaa cttttttaat gtataataaa gagctcgatc atttactaca
      661 aggatgctag ctcctacatt ttcacagttt tttagcaagt aaatttttct aaaatcacat
      721 caggaaatgc atgagattct ttaagagagg caattatagg gctatgatta cggttcgtgt
      781 aagtgacgcc aaaaaagaat ggctttctta tatggctgaa ttttatggga ttactttatc
      841 tgaacttcta aaaaactatt cgatggaaca actagaagat gaatacaatc gtcaaacggc
      901 tgaaattgcc cataaacatt ggataagaag tgggaaaaca acagtattca tggaagaatc
      961 cttaatgaat ttggtgggct ggaatgacct gccgtttaaa attcacgccc gaaattcaga
     1021 agcagctctg attggcaaat acgtgtattg gtagagatag aagataatca attcgttgtt
     1081 atcgctattc aagtagggca ccgaagaaat gtctatgatt aagagactgt ttatttttac
     1141 aaaaaaataa taattggaat ctagttaaaa ctcttgaagt gagagatttg actagatttt
     1201 tttatgaata aaataaggac aaaattgttt aggaagaatc atgagtgtaa aaaaatcatc
     1261 caagaagtcg attcttggat tttttagtgg atttgttcgc caaatacgtt tagtatgcgt
     1321 atatgagagg ttgtaagcaa agcgttgtgg tctgagcact taaggaggaa tctgtgaaaa
     1381 tagcttttca tatttttgca gattttgact taatgccgaa ggtcagcttt gcgattaccg
     1441 tttatcgaga ggctgtaaaa gcaccgtgta gctccgatga caataagcaa gcatttttag
     1501 agctaggttt ccttgattct aaatatgcgg cttatttcaa tgagttggtg cttgaacgcg
     1561 attttaagaa ggtcgaaaac aggcacgact tttcgacgta aggagaaaga atttcaatga
     1621 tagaaacaat aaatcccgca gaaaacaagg tttctgcgta cattcatatc ccattttgcg
     1681 agcatatctg ctattactgt gactttaata aagttttttt agaagggcag ccagtagatg
     1741 agtatgtgga aatgctgctg aatgaaatga gattaatgct ggaaagtcat cctacagcga
     1801 atttggaaac actttatgta ggaggcggaa caccaacttc tttatctgct aaacaacttg
     1861 atcgactatt atcaggtgct agagagatcc tgccttttca aaatggtaat gagtttactg
     1921 tagaagcgaa tcctggtgat ttgacccgtg aaaaacttca agtgatgaaa aactatggcg
     1981 tcaatcgttt atccatggga gtgcagacat ttgacaatcg cttattgaaa aaaattggac
     2041 gtaaacatac tgcagaagat gtgtatcaaa caatgcaatt tttagaagca gaaaatttta
     2101 ccaatgtaag catcgatttg atttatgcat taccgggaca aacactagaa ggatatcggg
     2161 atactttgga aaaagctttg gctttggatc tgcctcacta ttctttgtac tcattgatcc
     2221 tcgaaaacaa aacaatgttc atgaactggg tacgacaagg aagactcgaa ctgcctgatc
     2281 aggaaacgga aaccagaatg tttgaagaga cgattgaagc aatggaaaag catggacgtc
     2341 accaatatga aataagtaac tttgggctct ccggacatga atctcaacat aacctcatgt
     2401 actggaacaa tgaccactat tttgggtttg gagctggtgc tagcggttat cttggagata
     2461 ttcgttaccg caacaaagga ccaatccaac actatttacg cccattacga gcaaatgaac
     2521 gcccggtatt ggaagaagaa aaattgacac gaaaaaatca aatagaagaa gaaatgttct
     2581 taggactaag aaaaaaaaca gggattacta aaaaacattt ctatgatcgc tatcaaaaaa
     2641 caattgaatc ggtgtacggt gatgtgctaa gggaattaga gcaagaaggg ttacttgtta
     2701 atgaagaaga ccgtgtttat ttgacaccaa gagggacttt tttggggaac aatgtgtttg
     2761 agcgcttttt atttgaaaaa tagtttgaag aatatttgtt ttttatcgaa acttgaaaag
     2821 aaaaagttta gaaagtttga caatgaggta ttgaagtatt gatataactg gatttacatc
     2881 aattatttag aattagcact cttgtgttta gagtgctaaa aaatggagtc tttctcttga
     2941 caggagaagg gcttcttgct atatttataa gtgaattagc acttagaata tatgagtgct
     3001 aatgagaggt gaggaagatg ttaacaaagc gacaaaacga cattttacgt ctgatcatcc
     3061 aacactatac gagtagtggc gtgcccgttg gttccaaaac attaatgtct gagggagtca
     3121 aggccagctc agcaacgatt cgcaatgata tgaaagcatt agaagatgaa ggattgctgt
     3181 tgaagactca ctcttcttct gggcggattc catctgcttt aggctatcgt tattacgtgg
     3241 atcatttgtt aaaacctgct cgcgtagcag aagatgattt gctgctgatt cgtcagtctc
     3301 taggcagaga atttcatgaa atcaatgaga ttattaagca atctgctgag atattatctg
     3361 agctgacaag ctatacgact ttttcattgg gacctgaaat aaaagaccgc cgactgactg
     3421 gctttcgtat cgtacccttg aataatcgac agattattgc tatcgtcgta acagatatgg
     3481 ggaatgtaga aagccaagtg ttcactcttc ccgaatatat gggaagtcag gaactagaaa
     3541 aaatggtaca gattgtaaac gaccgtctag tcggagatcc tctgatgacg gtgtaccata
     3601 aacttcgtac ggagattccg atgattcttc ataaatattt ccagacgaca gaagggatgt
     3661 ctcagttatt tgacaccgtt cttggtcatg tttttgaaga aaaggtttat gtcagtggac
     3721 aaatgaatct cttggattat gagccccgtc aagatcttga tcagttcaaa tcgatgtttt
     3781 cttttatgaa agactcagac ggacttacac aaatgatcac tccgatggat agcaatattc
     3841 atatcaagat tggtgctgaa ttaggcaatg atttattgca gaatatgagt atgattcaag
     3901 caagttacga gatagatggt catggaagag gtacaatagc cttgttaggg cctactagta
     3961 tgccttattc tcggatgttc ggtctagtcg atgtttatcg aagagaacta gcagctaaac
     4021 ttgctgatta ctaccgctcg cttgacttgt caaactcgta atgtgaataa cgaagctatt
     4081 tgcagctgat cgttctctct ttcttagtaa gagcaatcaa acagctgact gaataatgaa
     4141 aggaagactg tatcgtgaaa gaaaatcaag aagaactaga taaagaaatg acagatgctc
     4201 agccagaacc ggaaatcgat gtcgaagctg cagaagattc gggtatttca gaagctgaag
     4261 cggaagaatt cgaaacagcg aaattaaaag ctgagttaga agaaatggaa gataaatact
     4321 tgcgggctcg tgctgaaatc gcaaatatgg caaatcgagg aaaaaatgag cgtgagcaat
     4381 tacaaaaata ccgttcacaa gatttagcca aaaaattact tccatcaatt gacaacctgg
     4441 aacgtgcatt agccacagaa gtgtcagatg atcaaggggc aggtttgaaa aaaggcgtgg
     4501 aaatggtctt ggaaagtttg cgtaacgcac tggaagaaga aggaattgaa aaaatccctg
     4561 ctaaaggaga agcctttgat cctaatcttc atcaagcagt acaaactgtt ccagcgactg
     4621 aagatacacc agccgatacg attgtagaag tactacaaga aggctacaaa ttacatgatc
     4681 gcgtgttaag accaactatg gtcattgttg cacaataatt gtgcggatat aaaacttaca
     4741 ctttcaatta aagaaggaga cgttaattat gagtaaaatt atcggtattg acttaggtac
     4801 gactaactct gcagtagcag tattggaagg aaacgaagca aaaatcatcg ctaacccaga
     4861 aggaaaccgt acaacacctt ctgttgtatc attcaaaaat ggagaaatcc aagtagggga
     4921 agttgcaaaa cgtcaagctg tgacaaaccc aaatacgatt tcatcaatca aacgccatat
     4981 cggtgaagca ggttacaaag tagatgtaga aggtaaatca tatacaccac aagaaatctc
     5041 agcaatgatc cttcaatata tcaaaggatt cgcagaagac tatttaggtg aaaaagtcga
     5101 aaaagcagtt atcactgtgc cagcttactt taatgatgca caacgtcaag caacaaaaga
     5161 tgctggtaag atcgctggtc tagaagtaga acgtatcgtc aatgaaccaa ctgcagctgc
     5221 tcttgcctat ggtttagata aaacagatcg cgacgaaaaa atcttagtat tcgaccttgg
     5281 tggcggtact tttgacgtat ctatccttga actaggagac ggtgtctttg atgtattgtc
     5341 aacagctggc gacaaccatc taggtgggga cgactttgat aacaaaatca tcgactacat
     5401 ggtagcagaa ttcaaaaaag aaaacagcat tgacttgtct caagacaaaa tggctttaca
     5461 acgcttgaaa gacgctgctg aaaaagcgaa aaaagattta tctggcgtat caagcacaca
     5521 aatcagcttg ccatttatca cagcaggaga atctggacca ttgcatttgg aaatgacatt
     5581 gacacgtgca aaatttgatg aattaacagc tgacttagta gaacgtacaa aagtaccagt
     5641 acgtcaagcg ttaaaagatg caggtctgtc acaatcagaa atcgacgaag ttattttagt
     5701 cggtggatct actcgtatcc ctgcagttgt agaagcagta cgtaaagaaa caggaaaaga
     5761 accaaacaaa tcagttaacc ctgatgaagt agttgctatg ggtgctgcta tccaaggtgg
     5821 cgtgatcact ggtgatgtga aagatgttgt tttacttgac gttacaccat tgtcattagg
     5881 tatcgaaaca atgggtggag tgttcactaa attgattgac cgcaatacaa cgattccaac
     5941 aagtaaatca caagtattct caactgcggc agataatcaa ccagcagtag acatccatgt
     6001 attacaaggt gaacgcccaa tggctgctga caacaaaact ctaggaagat tccaattgac
     6061 agatattcca gcagctcctc gtggtattcc acaaatcgaa gtaacattcg atattgataa
     6121 aaacgggatt gtaaatgtat ctgcgaaaga tctaggtaca caaaaagaac aaaaaatcac
     6181 gatcaaatct tcttcaggtc taacagacga agaaatcgaa cgtatggtga aagatgcaga
     6241 agcaaacgct gaagcagata aagctcgtaa agaggaagtt gacctacgta atgatgtaga
     6301 cgcattgcta ttctctgtgg acaaaacatt gaaagaatta gaaggcaaag tggacgaaga
     6361 agaagtgaaa aaagcagaaa cagctcgtga cgaattaaaa gccgctgttg aagcaaacaa
     6421 catcgaggaa atgaaaacta aacgcgatgc attgaacgaa atcgttcaaa acttgactgt
     6481 taaactatac gaacaagctg cacaacaaca agctcaagaa aatccagaag cagctcaagg
     6541 cggagcagat gatgtcgttg atgctgattt tgaagaagtc gatggcgacg ataaataatc
     6601 agttcactga atttcgagag aaggccaaag ttgctacttt ggctttttct tttgaaaaat
     6661 agtttgaagt atcggttttt gtttcaaaca aaatatgata tgataacaag cgacgtgaaa
     6721 atgatgagaa ccaataggag gaaagcctat ggcaacaaaa cgtgattatt atgaagtctt
     6781 agggctctca aaaggagcat cagaagatga aataaagaaa gcttatcgta agctttcgaa
     6841 aaaataccat cctgacatca ataaagaacc tgatgcggaa gaaaaattca aagaagtatc
     6901 agaagcatat gagattttaa gtgacccgca aaaacgcgct gcatacgacc aatacgggca
     6961 tgcaggcaca gatccaaatt acggtgctgg tggagctggt ggcggatttg gtggattcgg
     7021 cggcttctct ggtggcggct tcggtggttt tgaagatatc ttcgactctt tctttggagg
     7081 aggcggtcgg tcagtcgatc caaatgcgcc tcgtcaaggt gctgatttgc aatatacggt
     7141 cgacttgtct tttgaagaag cgatttttgg tgtagaaaaa gaaatcaagt ataatcggga
     7201 agaaatctgt catacttgtg gtggaaacgg tgcaaaacca ggcacacaac caactacttg
     7261 tcataaatgt catggtgcag gtacgatcaa tgtagaacgc caaacgccac ttggacgagt
     7321 gatgagccgt caaacttgtg atgtttgtca tggcacaggg aaagaaatca aagaaccttg
     7381 tccaacctgt catggctcag gacatgagaa gaaagcacat tcagttaaag tgaatgtacc
     7441 agctggtgta gaagaaggac aacaaatgcg tttagctggt caaggagaag ctggagaaaa
     7501 cggcggacca tttggtgatt tgtatgtggt ttttcgtgta gaagaaagtg atatctttga
     7561 tcgtgaaggt tctgaaattt actatgaatt gcctttaagt tttgtacaag cagctttggg
     7621 ggatgaagtc caagtaccaa ctgtccatgg agatgtgaaa ttgaagatcc ctgctggaac
     7681 acagacaggt acgaacttcc gtttacgtgg aaaaggcgct ccgaagttac gtggtggtgc
     7741 aacaggcgac caacatgtca aagtaaaact gatcacaccg aaaaatctaa atgaacaaca
     7801 aagagaagca ttacgtgcat tcgctgaagc tggcggcatc aaagttgaag aacagcaaga
     7861 agacggtttc ttcgataagt ttaaagatgc ttttggcggg aaaaagcgga agtagttttt
     7921 ctagtaaaaa tataaagtag acttgcccaa attttctatt tctagtaaaa acgctaagtc
     7981 attaataatt aaaaaccaga cgaacaatca tacgttcgcc tgg
//
LOCUS       DAZAID010000114         7966 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000114 DAZAID010000000
VERSION     DAZAID010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.117"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..578)
                     /locus_tag="ACG9BE_002541"
     CDS             complement(<1..578)
                     /locus_tag="ACG9BE_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN3033251.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENG"
     gene            complement(588..1964)
                     /locus_tag="ACG9BE_002542"
     CDS             complement(588..1964)
                     /locus_tag="ACG9BE_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3033252.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(2084..2731)
                     /locus_tag="ACG9BE_002543"
     CDS             complement(2084..2731)
                     /locus_tag="ACG9BE_002543"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFN3033253.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(2815..4242)
                     /gene="uxaC"
                     /locus_tag="ACG9BE_002544"
     CDS             complement(2815..4242)
                     /gene="uxaC"
                     /locus_tag="ACG9BE_002544"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFN3033254.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(4316..5395)
                     /gene="uxuA"
                     /locus_tag="ACG9BE_002545"
     CDS             complement(4316..5395)
                     /gene="uxuA"
                     /locus_tag="ACG9BE_002545"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN3033255.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(5398..7029)
                     /locus_tag="ACG9BE_002546"
     CDS             complement(5398..7029)
                     /locus_tag="ACG9BE_002546"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFN3033256.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            7339..>7966
                     /locus_tag="ACG9BE_002547"
     CDS             7339..>7966
                     /locus_tag="ACG9BE_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3033257.1"
                     /translation="MKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQKK
                     RILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERFRW
                     LRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNI"
BASE COUNT         2597 a         1586 c         1067 g         2716 t
ORIGIN      
        1 caaccatttt caattacact tcgaataata tcttcttttt ttcctttatc ttgacaacac
       61 agttcatcaa ctaatgataa taattcatgt ctttcccatt cttcttgaac agcttcctta
      121 ttagctgagc ttacaataat tatcttccca tattttttca gagcttttat agtatctcta
      181 gcacttaaaa agggtttatc taatcctttt gcttgactaa tctgttgatt gacttcttcc
      241 gaccatttta aagcttttaa taaatcctct gactgagaat attctatttc cctcttcaaa
      301 gatggattag acaattcttt tgttagagat acccagtgct ttaatcgatt aatcgatgtt
      361 acatcatttt tttctaatgt tttaactaaa cctaaaaatc tgtttattcc acgagtctct
      421 gaatataaat taatattatt ccaatcctgt aggaattttt ctctatgatg aacatcaaaa
      481 atctctgcag caattggtcc gaaaaagaga ttgtgtttat aagtcatcgt atccataaca
      541 cagccatctg aatcaataca aaatatccaa ttattcatat ttctctccta tttatgtttt
      601 tgttttaaaa gctcgcaatt ttctaagact ttatctttag ataatggata aaggaaaaat
      661 aaaattaatc ctacaatcag aaaacaaatt gatggaatta atgttgaagc gttatataac
      721 ttttctaaaa catctcccgt ttgtacattt gctaaagagt catagcctat aatagaaaga
      781 gtccaacctc cgacgcctcc agctagagct tgacctattt ttctagcaaa agaatatacc
      841 gcatatacgg tcccatcttc tctttgatca gactttactt cttgatcatc aataacatca
      901 gtaatatttg cccaaataac agtattaaac acacccaaac ccatatagcc aaccgaacta
      961 agaattaaga atacatacat attactagct tttatcaaaa ataagatcaa atatactatt
     1021 gctgaaaaga acattcctac tgatgccagt tctttttttc catatttttt acttaaaaac
     1081 aaagttaaag gcgaaacaac tattaaaacc aaaataggat tgataaaatt cattaaaact
     1141 attccctgtg catcattata gtaatcagga aatacataat tattcatact cataattaat
     1201 aattgagcaa ggagtaagaa aatagccgca ccaattatag aaagtaatgc cctattttta
     1261 actaaccctt ttaaagattt taacaatgaa actttgtatt gagattggtc aattcttact
     1321 cgttcagtag ttagtttata acatattaaa taacaaataa gtgcacatat cgaaaatatt
     1381 cccgcaacaa ttgtaaaagt tgatccacca cgaactattt gagcgcctga atcatctctt
     1441 gtataaataa ataatggtgt taatacacca atgaccaaac ttgctaaagt agctccaaca
     1501 tttcggaatg tcgatagcat tgttctttct tctgtaatag aagtaatagc tgatgccatt
     1561 gatccataag gaatatttat agatgtgtaa aaaatactcc cccataatat atacgtaaca
     1621 tacatgtata ctaccttaac gctcatagaa gctgaaatca tagaagtttg atacattaaa
     1681 aaacttgcta aagcgactgg accactcatt cttaaaatcc acggtttaaa cttaccctgc
     1741 ttagttgctt tcgatctatc aacaatacgc cccatagtta catcagtaac tgcatcgata
     1801 aatcttgcta ctaaaaataa tgttccaacg gcagctccgc ttatccccaa tacttttgta
     1861 taaaagatca ttaaaaaaga actagcaaaa atgaaagtaa aatcgtttcc aaaatctcca
     1921 aacatatatc ctattttgtc tcttatacca aattttcttt ccatacaatt ctctcccttc
     1981 tgttgttaag cgttttcatt ctgatgaaag tacgtactat actctgaagc gtaaagagtc
     2041 agtatttatt ttttacattt cagagtatta tttgtttttt ctcctaataa ttctttatct
     2101 cataaaactt atctatatat ttactagcta ctcgacttac ttcttcaaaa tcaccttttt
     2161 caacagaagc caataaattc ccacctacac caactgcaac aactcctact tcaaaccaat
     2221 cttctaagtt ctctatactt actccgccag taggcattat atttacgtgt ggtaatggag
     2281 cttgataggc ttttacaatg gatggaccaa aagcagaacc tgggaatagt ttaatgatat
     2341 ctacgccagc agtaagtgcc tcctttatct cagtaattgt catacaacct ggtaaataag
     2401 gaatttgata taaattacaa atctcagctg tttgtcgatc aaaagatgga ctaacgatat
     2461 attctgcccc agccataatt gcgatacgag cagtaaacgc atctaaaacc gtaccggctc
     2521 cgattattac atctttttga tcagcatatt ttttagcaat ttctttgatt atttcttcag
     2581 cattaggaac tgtaaaagtt agttcaatcc ctttaattcc acccgcaata attgaattta
     2641 ttgctttaat ggcctcatcc ttatttttcc cacgtacaac cgcaatgact ccagccgctt
     2701 ctaacttact taaaatttct acttttttca tatctatctc cttcctttat ggatacatac
     2761 tttgtctagc tgtactaact tacaaagaat aaatttaagc ttttatttca agtattattt
     2821 tctacttccg atttcaaaaa aattaaaata attatacgca ttacgataac aaatattctc
     2881 cactatctga ctaagataca tttcatcatc tggaattcgg cctgctttaa cccactcgct
     2941 tagtaattga cataatactc gacgaaaata ctcatggcga ggatagctta gaaaacttcg
     3001 agaatcagtc aacatacctg taaaattcga caacaaactt tgttgtgcca tcgtagtcaa
     3061 ctgttgacgc attccttcag ctgtatcatt gaaccaccaa gcacatccca attggatttt
     3121 ttgaatcata cccccttgga agctttgcat tgctgttgct agtggaagcc aatcagttgg
     3181 atttaatgaa tataaaattg tttttggtaa gcacccactc ttttcagcgg tatcgaacaa
     3241 tgctacaaca ttttgtgtca aactagcttg atcccccatc gaatcaccac cgacatctac
     3301 tccaatttgt tcctttaata tacttgaatt atttcttaaa gcatttaaat gcatttgcat
     3361 cgtccaacca tatcttgtat actcagacat caaatgtaat tgaatacctg taacaaattg
     3421 tgcaatttct gataaatgga aatccgtttt tcctgctaaa gcttttatta acagattgtc
     3481 gagtattttt tcatctgcta tttcataaaa ataagtattc aaaccgtgat ctgctaatcg
     3541 acagccaatt tgatgaaaat gatccactcg ttggctcaat gcttgcttca acccattaaa
     3601 gtctttgatt ggtatagccg ctgtttgact aagttttaaa atatagttcg aaaagcctga
     3661 tgatttgata ctccagacct tatctggacg gaaagtaggt aataccttga aagaattatc
     3721 ctgtttagaa agctcagtat gataccacaa gtcatctgta ggatcatccg ttgtacagat
     3781 ggctttcaca tccatcatct ccaacaattc tttcgggcga taggaatccg tttgaatttt
     3841 ttcgttagca cgtcgccaaa tttctggtgc attcttctca gaaattgtca aatcaatatc
     3901 aaagtaacgt tgtaattcca aatgagacca ttcaaaaata ggattacctg gcgccttctc
     3961 cactgttttt acaaaagcta aaaatttttc ataatcatca cctggaccac tgatcaactc
     4021 ttcttctata ccattagctc gcatcaatcg ccatttataa tgatctccag ctcctttatc
     4081 atttaaccat acctgagtca aattcttgta ttgcttattt tctagcaaat atgctgggtc
     4141 taaatgacaa tgatagtcga taattggttg gtgttttgca aatttatgaa aaagtctttt
     4201 agctatttca ttctctaata aaaagttctt atctaaaaac atatttaaac actcctttcc
     4261 ttccgttagt ttttagaaga gaaaattttt cctctaaata aactccctta tataattatt
     4321 tactcgcccc cttttcaagc gcttcccaaa taccatttaa ataaactgca cctagagctc
     4381 ggtcatataa tccataacca ggtctaccgg tttctcccca aatcatccga ccatgatctg
     4441 gacgaatata gccattaaat ccattatcgt acattgtttt taatatctta tacatatcta
     4501 gagaaccata ttctgattta tgcggcgctt cgtaaaaatc gagtccattg tcacttaaat
     4561 atttgatatt acgaacatgt gcaaaaggaa tccgatcccg ttgacaaaat tcagatagaa
     4621 tttcatagac atcattatct ggtcgttctg caatacttcc agtacataat gtaataccat
     4681 tagaaggaga atccactaca ttacacaacc aatctaaatc gtcacgattt ttaacaatac
     4741 gaggtaatcc gaaaatggaa tatggtggat catctgggtg aactgccatt ttaatcccaa
     4801 cttcttcaca tgtcgggata attgctttga gaaaatatgc aaagttctct cgtaatttct
     4861 cttcatcaac ttctttatag gcttctaata attgcttaat ttgtgataac cgatgaggtt
     4921 cccaacccgg caattcaaaa ttattagtag aattttctac ttctctaata atttcctccg
     4981 gatcagtagg gatatttttt gaaatatagg ctaacgtaga agacccatca tctaaacgat
     5041 aatccaaatc cgattttacc caatcaaata ctggcataaa attataacag ataacctcaa
     5101 tcccaaattc tgccaaattg cgaatcgtct gtttataatt ctcaatgtat tgatctcgtg
     5161 ttggtaaacc aattttgata tcatcatgaa cattaacact ctcaataact ttcaaggtaa
     5221 gtccattggc ttcgacttca tcttttaaag atttaattcg ttctctcggc caaacctctc
     5281 cggctggtat atccattaat gttccaacaa ttcctttcat tgctgggatc tgacgaattt
     5341 cttgtagtga gattttttct tcatttgccc cataccatct aaaagtcatt tccatagtta
     5401 ctttcctcca acttctgtta tatatttatt gattgtttta cgaactgctt gaggacctac
     5461 tagcatttcg caaaagattt tttcagtttt ctttcctacc ccaacttgat ataaatctaa
     5521 accaaagatt tctttatttt ctaaaatttc ctgtaccgca caatgaatct ttgaaggatc
     5581 ttgttcacct aatttcaata ctgataattt atcctgcaac tctaccaata acggatcagg
     5641 acttggcaca aaattttccc cttgatcatc gactgccagt aaataacgta accaagcagc
     5701 aatagcgata ggaataaatg ttagattttc tgcatttccg tctttttcga catacttttt
     5761 aattgtttca ccatagcgaa tcgctacttt ttgagaagta tctgttgcaa ttcgttgtgg
     5821 tgtatctgga atatagggat taggcaaacg tttattaatt acttcatcaa taaattgttt
     5881 tggatcaata atgcctggat tttcaactac tggtaaacct tctaaataac ctattccttt
     5941 gattaaacca accaaatcat catcctgcat ctctgctgaa attgattgat atcctagcag
     6001 acaacctgtt actgccaaag cagtatgaag tggattcaag cttgtcgtaa ccttcatgat
     6061 gtctgcttta tcaaccgtct ctctatctgt cataatcaca ccagcctttt ccaatttagg
     6121 ccggccattt ggaaaactat cttcaataac taaataatga actacctcag tattagtgaa
     6181 agcagcaata ttggtatgtt tttttgtgtg aatgatatct aaatcctcga gccccatcat
     6241 caatagatct tcctttacat tctctgaagg atttggcgta attcgatcaa tcatactcca
     6301 aggaaaagtc actttttctt tatcctttaa ataagcaata aatttttcag aaacatatcc
     6361 ttctttttgc caagcttctg caatcgtaaa aatactatct tgaaaacgtt gaccattctg
     6421 tgagaaatta tcagtagaaa ccatcgcaat cggcaactct ccagcattaa atctgctata
     6481 taacaaggat gttacaatac tcatcgtatg ttgaggattg ctaggtccag atttgatatc
     6541 agctttgact accgaaaaga attgcccttt agaatctttt aaactgtaac ctttttcagt
     6601 aatagtaaac gttgtaaact gtaatttagg atcttcaaaa tatttaacca ctctttgata
     6661 gtccgctata ttcttcggat tgcaatataa actatcagcc gttactgcca aaagtctttt
     6721 tttgagttca ccatctccac tcataacaat ttccaaaatg tcattgttat agtcatgata
     6781 agctttttcc actacttctt catcaaatgt ttcacacaca actactcctg tttctaattc
     6841 attttgatct gctaagcatt gagcaatttc tgcatgaaat cctcgataaa gatttcctcc
     6901 accaaaatga agccatactg gatgttgctg gccttcttgc ttaacttctt tgacatcaaa
     6961 acacggcagt agaatctgca tttcattgaa ttcttcgata gaattttgcc aatttttaat
     7021 cgaaaccata attaacctcc tcaaactgat atgttagttt tgatacattt tatattttaa
     7081 aacccattgt aaccgttgtc aatataaaaa gggaataaac ttgctttttt ctaccatatc
     7141 agaatgattg tattttttat tcagaaaaat aaaatactcc ttctttttgg actgatttta
     7201 tcgaaactta ttttttcttt ttttcatgta ttcttttatt tttataacta gtgtattact
     7261 tcaaaatttg atattatatg gcatgaacag aattaaaata tcagttaaaa taaaagttaa
     7321 ggagcattgt gtatgacaat taaaactagt ctccaaaatc aagcttacca atctattcgc
     7381 cgacaaatta tctatgctga cttagaacct ggaaaaaaag tatcagaaaa aaaattagaa
     7441 gaaacacttt ctattggtcg tacaccaata cgtgaagcac tgattcaact ccgacaacaa
     7501 ggtcttgttg atacaattcc tcaaagcgga aattatatct cattaattga tttagattcg
     7561 gccaaaaatg ctcgatttgt cagagaacat ttggaacgac aaattatgtt agagtgttgt
     7621 gccaaaatga ataaccaaaa aaaacggatt ttagaaacta taatttcaga acaagaaaaa
     7681 gctgttagtc aaaaaaatgc tcgtagtttt tttcataatg ataatttatt ccataaagct
     7741 tgttttgaaa ttgcagagcg agaagaagta tggcaatggt tagaaaataa caatacacac
     7801 ttagaacgat ttcgatggct acgagtaaaa acggaaggat taaagtggga aaaaattatg
     7861 gaacaacatt atcaactatt tgaggcatta acaaaacaag atccagaaga agctaacttt
     7921 ctaacatcgt tgcatcttca tttaatgctt aatgagcaaa atattg
//
LOCUS       DAZAID010000115         7813 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000115 DAZAID010000000
VERSION     DAZAID010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.118"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..1385)
                     /gene="celB"
                     /locus_tag="ACG9BE_002548"
     CDS             complement(33..1385)
                     /gene="celB"
                     /locus_tag="ACG9BE_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN3033258.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVTLGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1385..1903)
                     /locus_tag="ACG9BE_002549"
     CDS             complement(1385..1903)
                     /locus_tag="ACG9BE_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033259.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1919..2245)
                     /locus_tag="ACG9BE_002550"
     CDS             complement(1919..2245)
                     /locus_tag="ACG9BE_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFN3033260.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2258..4237)
                     /locus_tag="ACG9BE_002551"
     CDS             complement(2258..4237)
                     /locus_tag="ACG9BE_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN3033261.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4258..4572)
                     /locus_tag="ACG9BE_002552"
     CDS             complement(4258..4572)
                     /locus_tag="ACG9BE_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFN3033262.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4740..5033
                     /locus_tag="ACG9BE_002553"
     CDS             4740..5033
                     /locus_tag="ACG9BE_002553"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFN3033263.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5111..6223)
                     /locus_tag="ACG9BE_002554"
     CDS             complement(5111..6223)
                     /locus_tag="ACG9BE_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN3033264.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6376..7224
                     /locus_tag="ACG9BE_002555"
                     /pseudo
     CDS             6376..7224
                     /locus_tag="ACG9BE_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7577..>7813)
                     /locus_tag="ACG9BE_002556"
     CDS             complement(7577..>7813)
                     /locus_tag="ACG9BE_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN3033265.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2496 a         1553 c         1262 g         2502 t
ORIGIN      
        1 attttttgtc acagttcctt ttttcttaga aatcatttta acttttcact agctaattct
       61 gctgcctttt ctctttcttc atttaataat ttattatcat agaatttgat aaatgggaac
      121 cagataatga atgcgatgat tgcacaaatg actgctaaca cagcagcttt ccaatcacca
      181 ccactaccaa caaatgctcc gatcccgact ggtgaaggcc acggcatctg tgcaagcatc
      241 ggtttgacga tttccagccg aatggcaaaa taagcgagtg tagcagaagc catcggagct
      301 aagaaaaacg gaaccgctaa atagggattg tagacgattg gcatcccgaa aatgattggt
      361 tcattgatat tgaataatcc tggtacaagt gaggcttttc ctaacacttt cagctgatca
      421 gatttcgcga ggaatgcaat aaagatgatc aatcctaaag tcgcacctga tccaccaacg
      481 gttacataag aattgttgaa ttctcctgcc aacggataat ttgcgccttg tgcgttagct
      541 gccatattag ctaatacgat cggggtcaag aaagaagtga tgatattcgc tccatggatc
      601 cctacgatcc acaatgcatg gatcaagaaa taaatcacca tgacaccaag ccatgtattc
      661 gtcaactgcg taacaaaccc aaatgggata gcaatcattt tgaagatatc cgttcctagt
      721 gtaactaata atccattgat gatcatcacc acaaacgcaa tgacaaatgt tgggatcaga
      781 gcagtgaaag aacgagaaac tcctggcgga acaacatctg gcatttttat gacccaattt
      841 ctcttgacac acatacaata caattcgacc gcaataatcg acataataat agctgtaaag
      901 attcctgaag ttcctaaacg ttcgacaaaa gagcccatcc taataccatt tactacagtt
      961 tctgtctcat tgatagaagc aatcaagctg attttaccat cagtcatgat caattcaggg
     1021 atacacataa agaatgcaaa aacagatagt aatgcccccg tgagcggatt aacatttaat
     1081 ttttgttcat ctcgttcaat tgacgttaat tcataagcaa acacaatggc aaagtaaata
     1141 gataaaatcc ccatcgtcat tgtattgacg atcatgtaca agtcactaac tttgaaaaaa
     1201 gttgcattaa aaaatccttc taaaaatggg aacggtaatg gcaaaatatt taaaaccaaa
     1261 aacatcgagc ctacaatcgt gaaaggaata gaagccatcc ccgctgccat gacagcccga
     1321 acgattttaa gttgcgcaat tttccccata ggtcccatca ggtatttctg caaaaaatca
     1381 aacattagtt attctccttt cctaaatata acgctttttc atattgttta atacggatat
     1441 actgttggtc ttggttgagt gaaattttct ttaaacgata ctgtataaat gcactggcaa
     1501 aaataccgct tacaacaaga atgaaaacaa ataactgtcc tccattgtta tttgccataa
     1561 aaggatacaa gaatgtaaat cctgaggtaa acaacactgg aatcactaat aaattaaaga
     1621 cactctggaa caaaaaatac cactttgtcc atggaacgtt cgtttggtgt tttcgataaa
     1681 gctttacttg ttctcctact gcaggcaaaa tagctaaaat cagaatcatc ggtaaactac
     1741 ttcctaaact tttagctgat agaagcagaa tcatccattg catgttcatg aaaaagaaaa
     1801 aggcagtcag gtaacgtacg attagataac gattgaaata catgtttttc aaactaagtt
     1861 gctttttttc taattcgatt ttccttttta ctgtatcctg cacaatgatc cttcctttct
     1921 atttattttt ttgatacaaa taattcattt cctgtgcaac ttccaataat gtcatcgtcg
     1981 tcatcagatg atcttgtgca tggaccatga taatttccat ttctattttt gtcccgctgg
     2041 catattcttt cagaagttga gtttgtgact gatgcgcatg aagaatctct tcatttgctt
     2101 gtcgcaactt ctcttctact tcttcaaaca tctctttacg catcgagcga aatgcttcat
     2161 gtatcagtgt ccgtgcattt ccgctatgga aaatgatatc aaaagaagtc acttgtagtg
     2221 tttcactgtt tattttttca gccatctgtt ttcctcctta ttcgatcagt ttgataaatt
     2281 tactgctgaa attctcaaaa ataggttcag ctaagatttc ctgctgtact tttagctggt
     2341 caactaacct aacgattgct ttagtcacga ccgtaattcc ttcattttcg acataagaag
     2401 gcgaaactaa aaaaacaaaa tgaatttctt gatattgatc ccactgcatc ccagaaggaa
     2461 tcaatgcaac ggctaccttt gtttcgattc caactggaac agcgggatgt gggactgcca
     2521 ctgtatcact gaatatgatt tgtcccatat tttcacgttg ctggatctgc tttcgcattt
     2581 ctcttatata tccttcttct tcatttatgc tcagcttttg cagcaaatca tgaagaattt
     2641 cttccttttt cggttcgtac gtataagtct tgaaaaattt ttcagacaac tgttcggtgt
     2701 aaatctgctt tttttgctgt agagaaaatg ctggtatcgg tctatctgag gaatgatagg
     2761 gtatagattc tccaactacc tttcgtattt tacgaacatc atcttcattc aaaaaaatac
     2821 tgacatgtaa aactggaatt ttgaaaaaca ttgtcgaaag atcaatagaa gaaataatca
     2881 ggtcgattcc ttttaaagtt gtttcattga tctcataata gccttttaca cttaccactg
     2941 taatattttt accgaattca ctgactacgc gattctttaa tagttgggca ctgccgtaac
     3001 ccgttgcaca gataatcaat gcatgtagtt tatgcgcagc tctttctttt tccaatgccg
     3061 ccatgagatg caaagccaaa taggcccatt catcttcatt gatggtatag tttttcaaag
     3121 ccggcatgtt gctgaaatgc ctttttactg cgtcaaagac tttgggattt tctttcttta
     3181 tctcctcagt caatgggttc tcaatgcgaa tacctcggct caatcgaatc atcatcggtg
     3241 acaaatgatc gagtagtcca ttgaaaagct ggagatcctc tactaatgaa aaatgaaatt
     3301 cctgagacaa tcttttcaga actttcgcta attcctttga tagttcctga tcattttgat
     3361 tttctttgtg attcgactta gccatgagat gtaatgtcaa ataagcaatt tcttcttcgg
     3421 gaaacgatac acctgcgatt acctcaatac gttgaacgat ccgctgcgcc acttgatact
     3481 ctactcgctt agaaacttct tgttcgatgc ctaattcctt gatttcaaac ccttctcgga
     3541 gccgtttgat ccccaaagcc aagtgtaaag tcagattctg tatgataaaa tccgaaactt
     3601 ttaacttagc ttctcttgtt tcatcgagaa tgataatgac tagctcttca aaactgatgt
     3661 ctgggaaaaa acggctttgc cctaaatatt ctttcaatga atttccataa tggtttccaa
     3721 aaaaagtatc catgataaaa tgccgtcgat ttctttcttc cccttcgatc catgtccctt
     3781 gaccatgctt agaaacgatt tttagtgaat atggaatcaa tcgctccttg atttcttgca
     3841 tgtcctttgt caaactggaa cgactgatat aaagctcttc tgccaaatca tccataaaaa
     3901 gaatggcatc ttccaaaagc aattggttca atatatactt ttgtcgatct tcggactcat
     3961 aaaatccttg cggttctcta gcatatccag gtactatttc ttgccgactc aaaaacaagt
     4021 caaatgtcag tctttgtaaa atatgcagtt ggtagccata acctggttta gcaatgatct
     4081 tacctccgtt tttttctact aactctttga tccgctgcaa gtaattacgg attgtccgat
     4141 cagataataa caattcagaa gctaaacgtt gactggtgac atactccttc ttgtgatcaa
     4201 tcaaatatct caacagcaat ttttccttct gtttcattat catccactcc tattccatca
     4261 tatgttctct tcgatcacct ctgctaactt ctggatgcct gtagggattg ggacatacgc
     4321 ttggaaaggg atactaacaa caggctttcc aacagattct cctaattttt tgaatttatc
     4381 caaaaacatc gtcgtttgcg ggctgatcag aaataaatca aaatcactat tcttaatcat
     4441 attatcgcct tctgtagctg aaactgcatc tacgagtacc tcttctcttt tttgcttaaa
     4501 gaaatccgtt gtctttgagg ccatcatcga ggatgacatt cctgccgcac aaataatcaa
     4561 tgcttttttc ataaatcaat cactcctttt atttttataa accaagcata tcttgtaagc
     4621 gttttctatt caaaatatat atttccgtag cattgcggaa aaaatgtgta tcatatcttg
     4681 aataataatt tctggcaccg tgatatacta gtgatagaat tttttcagga ggggatttag
     4741 tgagtatcac agtgaatatt ttgtatacaa gtacaaatgg acaagccaga aaatttgcag
     4801 aagaaatggt cgaaagaaac attgtcaaag cgattcgtgc agaagaagga aacgagaaat
     4861 atgactatta ctttccacta gaagatccag aatctctttt actgatcgac cgttggaaag
     4921 atgaagaagc aatcgaaaag catcacaaat ccgaaatgat ggctcaaata aacgaactcc
     4981 gtaaaaagta taaattaaaa atgaaagtcg aacgtttcac tgatttaccg taaaaagata
     5041 cacatggaat gatagcaatt tatcatccat gtgtatcttc actttttcct tattttttat
     5101 tcgatctcat ctaatgattc aaaccgtgtt tttctcttat ttttcggata agtatttcag
     5161 tcaccccaat atacatgatt cctaaacata ccccaaggat attctcaaaa atgcggatcg
     5221 ttactgctcc tggaaccccg aataaactgg cagtgattgc caaagcacca aaagaattaa
     5281 aaactgtttt atatctatag gtagaacaaa cacctaaagc tagaccacct aatataccga
     5341 cccagttaaa aggaataaat tggctgatac caataaataa aacacatcca atcaatgagc
     5401 caatgattcg gtctactgct cgttctttga aaccaatcga catcaatcca tagctagata
     5461 caatagaaga aaatgcaaac cctgcccaca tcaatcgctg aaaagggaca tactctcctg
     5521 caacgatcaa aagactaatg ccaaatgcat agatgagcat ccaaattttt tcctgcgaat
     5581 acatgccttt caaaaaaatc tctttaaaca agcttttgtc tttgttcttt tctcgatgtt
     5641 ttctgtaaag gagaactgaa aaccaacaaa aaaacaaaaa taataagcta cttgtttgtg
     5701 taaagaaatt cttgttcaac gaatctttag gtaacgaata aacaataaag agatatgaaa
     5761 agccgtacaa agaagcattc cccattttgg gatcactagc ggttgccatt aatatgctgc
     5821 ttagtaaaat aaaatgaatc aacatgccca taactgacca tttgatcagc tggacatacg
     5881 gtgctactaa tagtgagaac atgacgattg ctaaacctat gatcgaatgg gaaatcttat
     5941 aaccaaagtc aacaaaacga atactcatca acatacagaa taaaaccaca gcgtatggtt
     6001 tatgcttcga ttcaaataat gttgtaatta taatgataaa acaaatggca aacaatacga
     6061 tgaataatga tcgtaaaaac aatgaacaaa tatacatgca cttttctttt ttagaagctg
     6121 cttctcgtat tttttgtttt aaaataaacg gatctagttg tagagactga taaaaatcca
     6181 cctgttgtcg tcctttcttt ttacttttat tttttacagt catctatgac aggttgtaaa
     6241 tactatggaa catcatatgg cgttgtcatt ttcttgtcaa ctatttttcc tattcatgtt
     6301 tttataaaaa aacatatcgt tttcttgtat gattaaatca ccacaaaata aacaactgag
     6361 agaacgatat gctttatgaa tggttctatc aaaaaaattc tgagatcaat agacaaaaat
     6421 ttgatgatta cagaggtctc ttacgaaacc ttacagaaga aaaaaacatt gatcgttgct
     6481 gcagttctct tccctgctcc atgtgtctgt agaaattgtg gttctgctgt ggtagatgga
     6541 aataggaaat taatggtggt gaaaaacaag aagaaggaaa ccattgtcca ctttgaataa
     6601 tataatcata tgccaatggt tatacgccta aaaaagcagc gctatacctg taaaaactgt
     6661 cgaagccatt ggaacgctca aagttatttt atccgtccaa ggcactcgat ttcaaatcat
     6721 gtcagacata aaatcacttc tttactgact gaaaaagtgt cgttattttt tatttcgaaa
     6781 agttgttagg tatctttgac aaccgttatt cgcacattga aagagttgag aagctattta
     6841 ccaaagtctt ccaaaagaat tctgtcttga gtgttgaggg ttgtgaattt cgttctcacg
     6901 tttccgttga ggataaactg agttttattt ccgcggatgg cgagacagaa aaattaatcg
     6961 agaaaattta cctacacaaa aaattcctcg attaacaaac tatttcctta gctgcactaa
     7021 tcctaaagaa atagaattct tagttacaga catgaacgcc acctactttc agctcaccaa
     7081 acatgttcta ccaaatgcga aattggtgat tgatcgatta cttggttttt ccacgccttt
     7141 caaggaggcg taacctttat tcacgagttg gttgaagctt tccgagacaa aagacccgac
     7201 ttgtttttct ccttattgac aaagagccat taaaactaga gaaaaaatta caactgatcg
     7261 ctgattattt gtagaaagaa aatcaaagta ttacccaata aacttgagtc attaggcaaa
     7321 aaacactgcg aaactcaaga atgattttcg cagtgttttt gtatattttt tattaactag
     7381 tattactaca aaggtttatt tttcacttct caacgcagca caaaattgtc tttacttatt
     7441 tactggttgg gttcaacctt ctctactccg atactaatat ttgtttgtat accccttcta
     7501 aaatcggtag tttccattca attgatgggt actagttgat tttaaaaact atgcttattc
     7561 aattttttat tttagctcat atatttcgtt tctttctaaa ttttatactg atgaaaacaa
     7621 gtagcacacc caatattgac aatataaact ggctaatgat ggtttctcca gtttttggaa
     7681 gatgcttatt tccactgctt gttggattgt ttcctcttat atctgagcta tcagcaactt
     7741 ttctattctc ttttgttaaa aagatcgcca cttcttgttc tttttcgatc ttgaaagtta
     7801 ctggtgtcgc atc
//
LOCUS       DAZAID010000116         7661 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000116 DAZAID010000000
VERSION     DAZAID010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.119"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            394..786
                     /locus_tag="ACG9BE_002557"
     CDS             394..786
                     /locus_tag="ACG9BE_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033266.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(959..1162)
                     /locus_tag="ACG9BE_002558"
                     /pseudo
     CDS             complement(959..1162)
                     /locus_tag="ACG9BE_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1128..1325
                     /locus_tag="ACG9BE_002559"
     CDS             1128..1325
                     /locus_tag="ACG9BE_002559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033267.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1368..1943)
                     /locus_tag="ACG9BE_002560"
     CDS             complement(1368..1943)
                     /locus_tag="ACG9BE_002560"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFN3033268.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(2098..2624)
                     /locus_tag="ACG9BE_002561"
                     /pseudo
     CDS             complement(2098..2624)
                     /locus_tag="ACG9BE_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3081..4592
                     /gene="gadC"
                     /locus_tag="ACG9BE_002562"
     CDS             3081..4592
                     /gene="gadC"
                     /locus_tag="ACG9BE_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFN3033269.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4605..6005
                     /locus_tag="ACG9BE_002563"
     CDS             4605..6005
                     /locus_tag="ACG9BE_002563"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFN3033270.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(6170..7605)
                     /locus_tag="ACG9BE_002564"
                     /pseudo
     CDS             complement(6170..7605)
                     /locus_tag="ACG9BE_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2433 a         1258 c         1351 g         2619 t
ORIGIN      
        1 gataatgaga tagaatataa atgtcataat attatacaat ctaaacaccg tttccttttc
       61 tatttttgat gacatcagta tatagctact cgaaagagta gtctttttat tattctcgct
      121 tattattgat atgaaaattt cgatttgaca aaaggtaccg aataattttg taatatgaat
      181 tattatcaag aagtaattat gatgataaca tatggaaact agttagtaat aataacaggt
      241 gattaaactt ttgaacgact tgtaattcta tattttttct tttaagtaaa aaatataggt
      301 gatttttttt gacttcatat ttataataaa gacacgtaaa tcaattattt ttcattttac
      361 tttaactaat aatgtttatt tggagggatt attatgttag atgatagatt gacgtgttct
      421 aattgtggta cattatttta ctatgaggaa gttagaaata ttgtaaaaca tagaaaaaaa
      481 gaaatgccta tacgttgtcc aaattgcaat gaagttatta agcgaaagtt gtctcatggt
      541 tattttgtca gctacgaact atatgaagaa agtaaaataa atctgaaaaa aaaaccaaaa
      601 tactataaaa atttgaaaac tggagctatt ataactgaaa aagaatttaa aaatttagtg
      661 actgaagaag aaagcaggtt ttataatttg tctggtaata tttgccctgg atttatggat
      721 cagattattg aaaatgagtc aattgaatca attgaaaact atgttcctgt tgacttacca
      781 aaatagaaaa aataatcctg tttttacagt gaattatttt aattttgatt ttcaataaaa
      841 atatattaca ataataatcg taatagctaa tgaaacaatt tctcaatctt tttaccactc
      901 caaacaggga gcgtcaataa ttttgtgtaa ataactcgtc cttctgcaaa ataatgggtt
      961 actcagtaaa cattgaagct aatgtatccg tcacttgttg gaagccttta tggcttctat
     1021 ttaagaattt ttgattgtat gtatcaaaga tggatactag gaaacgctct agtgattctt
     1081 cattttgaaa ctgctctttt ctgcggctgt atttcttgat ttgtttattg aaagattcga
     1141 ttgtagaacc agaaggaatg gagaacactt ttgaacttta caacaaaact actgttgata
     1201 aattaattcc aattttagtt ccaactgatt taaatggtca tgatgtttta cttcgtttta
     1261 acggattttt aggtgaggat tatagatacc taaaaatttc tgatcctcaa cctttaacag
     1321 aataataaaa aacgtgtaat caattatttg attacacgtt ttttatttca atcttcgttg
     1381 tagtagtggt tgcgacatac cgaaaggtaa tcttcattac ctcctactac aatctgttca
     1441 cctgaataaa ctggaatacc atcaacaata cgagcattca ttacagcctt tttattgcaa
     1501 aaatggcaaa tagttttaat ttcctcaatt ttatcagcaa atgtcaataa tgcttcggat
     1561 cccttgaaga gattattttg aaaatcattc ttcaatccat agcaaattac tggtacttcc
     1621 tcattcatta cgataagtga tagatcaata atattctctc tacttaaaaa ttgagactca
     1681 tcgaccaaaa tacaatcaac ttttccgtac tgatcaataa tctccactaa ttctccgtac
     1741 acatccattt cagaagtaat tgcaatagct ggtttcgaaa cacctatacg actctcaact
     1801 tttccaaatc ctgatcgagt atctattgcg cttgtcatta tgataactct cttcccttgt
     1861 tcttcataat tatgagccac tttcagtagc tcaatactct ttccactatt cattgttcca
     1921 tatctgtaaa ataattgtgc cattaaattt attcccttct tttacaattc aattattcct
     1981 taattccatc tgcttttatg cagctgctcg tttattcctt gatatgtcta taacatctac
     2041 tatccctaaa accaatttat aagccattag agatggtatt cctgaacatc tctcatattg
     2101 ttttttgagt ggtatatcat ctgaaaaact gaaggaatca tgaaaatagc gacatactta
     2161 tcatttagag ttctaacttt aaatgcattt acttcttcag tcagcacttt agtcatgttg
     2221 gacatggttt ggggcgtgta gtgatgccca tacattttct caatcaagtc tgcgatttcc
     2281 gacatggtaa taccttttcg gaagaggtga atgacggtct cctctaatgt gtcattcgtc
     2341 cgtctataag caggaacagt ctgttgcttg aactcgccat tgcggtcgcg cggaatctgg
     2401 agatgaagtt ccccatactc ggtcttgacc gtacggtcat aggagccgtt acgagaatta
     2461 cccgtgttaa aaccaatgcg atcgtacttt tcgtaatcga ggaaagccgt taactccgtt
     2521 ttgagaagcg tattgacagc tttttccaaa tggacacgga ataatccatt caaatcgcct
     2581 ttgttcgcta gagtctttag gatttctgta gtaaaatcgt tcatatggaa gtcctctttt
     2641 ctgtgaatgt gttgtggtaa ctctattcta cagaagggac ttcctttttt ctatttacac
     2701 aaaatatttt acactctcaa caaaagtatc ttcgattaat acttgctcaa gttatgtgca
     2761 gctacgaaaa agtaggtacc gaggtacttt ctttttcgca gctttttttg gacttatgag
     2821 aagtacattc gctggaagaa tacgatataa aacgagcttt ttcactaaag atattaatag
     2881 agtgaaagaa acaagctttt gttgtcagtt ttttcacttt tctaaaaatg aaaataaaaa
     2941 attctggaag tggagaagaa ttttgcttgc tatactttgt cttgtagaaa agaggtagac
     3001 aaaataatgc aagctcaaat aagcgatttt ccataacctt ggtaggaatg acaaattaaa
     3061 taggaaaggg aagtaataat atgaataaaa aaaagttatc tctattcggt ttttttgccc
     3121 tgacagcttc catggttttg actgtttatg agtatccgac ctttgcaaca tcacaattac
     3181 atttggtttt ctttcttctt ttgggaggta ttttatggtt tctaccagtt gctttatgtg
     3241 ctgcagaaat ggcaacagtt gaaggatgga aagatggtgg gattttcagt tgggttagtc
     3301 aaactttagg agaacgtttt ggttttgcag ctattttttt ccaatggttt caaattacag
     3361 taggttttat tacaatgatc tacttcatac taggagcgct gtcgtatgta attaattttc
     3421 cagagcttaa tacagatcca tttatcaagt ttattggtgt attagtcatt ttctgggtac
     3481 tgactttttc acagttgggc gggaccaatc gtaccgctaa aattgctaaa gcaggatttg
     3541 tcattggaat tgttattcct tcgatcattc tctttggttt agcagcagcc tactttatcg
     3601 gcggtaatcc gattcaaatg gctattaatg cgaaagcttt tattcctgac ttttctcagc
     3661 tttcaacttt agtagtattt gtatctttta tattggctta tatgggcgta gaagcttcag
     3721 catcacatat taatgagctg gaaaatcccc aaagaaacta cccattggca atgtttttac
     3781 tagtgatttt ggccatcgcc ttagatgcaa tcggcggttt ctcagtagca gcggttattc
     3841 cacaacatga cttgtctctc agtgctggtg taatccaaac atttgaaacg cttattttac
     3901 attttaacag tcatttacat tggttggtaa aagtgattgc tctcatgatt gcctgtggcg
     3961 ttatgggtga agttagttct tgggtagttg gtccatcacg agggatgttt gcagctgctc
     4021 agcgaggtct gttaccagtt ttcttccgga aaactaataa acaagacgtt ccagttccgt
     4081 tgattatgat acaaggaatt atcgttacta tttgggcagc agtcttaact tttggtggag
     4141 gaggcaacaa tttatcattc ttagtagcaa tctccttaac tgttgtcatt tatctcgtag
     4201 gatatttact atttttccta ggttactttg tattagtttt taagaaaaaa gacttaaaac
     4261 gaacttacaa tgtaccagga aaaactgttg gcaaaacgat tatcggtgga attggtttta
     4321 ttttatcagt gttcgcattg cttatctctt ttgtaccacc agcttcaatt gcagctagtg
     4381 agcaccgaac ataccaaatc atactactta ttagctttgt agtgactgtt cttttacctt
     4441 ttatcattta tgaagtacat gataaaagtg ctcacacaac tattgaggaa cctactcacg
     4501 tgaaagctga agacgttaat cccgctgttt atccagctgc tagaggagag caccacgtta
     4561 caaaaaatga tgaacatatt ttaaaacatt aggaggaatt cattatgtta tacggaaaag
     4621 ataatcaaga agaaaaaaac tatttggaac caatttttgg ctctgcaagt gaggatgttg
     4681 acttgcctaa atataagtta aacaaagaat ccattgaacc acgaattgct tatcaattag
     4741 tacaagacga gatgttggat gaaggaaatg cgcgattaaa cttagctact ttttgtcaaa
     4801 cgtatatgga acctgaagca gtgaaattga tgacccaaac gttagaaaaa aatgcaattg
     4861 ataaatcaga atacccacga acaacggaaa ttgaaaaccg ctgtgtaaat atgattgctg
     4921 atttatggca tgctccaaat aatgaaaaat tcatgggaac ttcaacgatc ggctcttcag
     4981 aagcctgcat gctgggtggt atggccatga aatttgcttg gcgtaaacgt gctgaaaaat
     5041 taggtcttga tattcaagca aaaaaaccta acctggtgat ctcttctggt taccaagttt
     5101 gttgggaaaa attctgtgta tattgggatg tggaactgag agaagtccca atggatgaaa
     5161 aacatatgtc aattaatcta gatactgtca tggattatgt ggatgagtac acaattggta
     5221 ttgtaggtat tatgggtatt acttacactg gtcgttatga tgatatcaag ggtctgaatg
     5281 atttagttga agctcacaat aaacaaactg actataaagt atacattcat gttgacgctg
     5341 catcgggtgg cttttatgca ccatttactg aacctgatct agtttgggat tttcaattga
     5401 aaaatgttat ctcaattaat tcttcaggtc acaaatatgg tttggtatat ccaggtgtgg
     5461 gttgggtctt atggcgtgac caacaatact taccagaaga attagtattt aaagttagtt
     5521 acttaggtgg agaaatgcca actatggcta tcaacttctc tcatagtgca gcacaactaa
     5581 ttgggcaata ctacaacttt gtacgctatg gctttgatgg ttatcgtgat attcaccaaa
     5641 gaactcatga tgttgctgtt tatttagcca aagagatcga aaaaactggt atttttgaaa
     5701 tcattaatga tggatcagaa ttgccagttg tgtgctataa gctgaaagaa gatcccaatc
     5761 gcgaatggac actatatgat ttatctgatc gtctgttaat gaagggatgg caagtcccag
     5821 cctacccatt acctaaagac ttggatcaat taattattca acgcttagtt gttcgagcag
     5881 actttggaat gaacatggct ggtgattatg tacaagatat gaaccaagca attgaagagt
     5941 tgaataaagc tcatattgtt tatcataaaa aacaggatgt gaaaacgtac ggctttactc
     6001 actaaaattc ttcagagaaa tataaaagaa gttaattaac taataaaagg agttgtgatt
     6061 accacacagc tccttaaatc tatttgaata aatatatgat attcatttgt tacactctca
     6121 atcttagctg ttgttctatt catcattact cttattgtaa gtagttcagt caaatttgct
     6181 tgttcaattg atcgataaca gtatgaattg tttgaaaagt aatcggatat ataattgttt
     6241 tctcactacc taagccttct tggtataaag aactatcgca aagtataatc cgctgagtac
     6301 aaaagaaagt gtcgtctact atttctctaa gttgtttaca aatgatatta ctaatagaaa
     6361 agtgttctcc caaaatgtct tctaactgtt gtcgatagta taaataccta aagtctgacg
     6421 gagctactat gaacacttca attggagatt tccgtttatg ctcatttaaa agacttaaac
     6481 taatggttag tttacgaata gaattctcat tccaacgcag aaataaattg ttttgttttc
     6541 gccattgttc aagaatttca tgaacttcta agtacaaagc gcgttgctct tcatttaaca
     6601 agtaagtctt atcaggttga aatatctgaa tatcagccca aagtgagcgg ataaacggga
     6661 gaagtgcttt atcaaaaagt atctgatttt cttcagatat attcagagat aagataagtt
     6721 tttgactgag ctcaatcacc aaagggttat ctgctacatg gttttgataa acaacgttca
     6781 agtctttttg taacaattca aagttatcgc atagataatt tctagagttg aatagaaagt
     6841 agatataaag gcattcatct tcgtcatgac ataaagagaa agaaagttgc tctaatcctt
     6901 tctgtattag ctgaaacaag ggagtatgtc gggcaactct tttttcttcc tgactaaaat
     6961 atattgggtt agtcttagat ctctgcaaac cgatagcgat acctaataaa ataaattgtt
     7021 tttcaactgt agggtaactt cttgaaaaaa atgtttttct acataatcaa tcaattgttg
     7081 accgctttga ataattgatt tatttagctg gatcaactct gtccgatttg tctcatagat
     7141 tatcgttagt aacaaacttc gataatcctt ttcaggaatt tcaaaatggc catcagcatt
     7201 taagtactgc atttgtttaa aaaaattaat caactcattt cgtaagttgt aaactttgct
     7261 taacgaaatg aaggtagtat cagctatatt actccaattg tgttcccctg ataaataatg
     7321 tcctagtaca ttgaaaaagt cagagcgctg atagatgagt tgagtgagtg ccaacctcga
     7381 aaacgaagag tcgaatgcga ctgtaaagct acttctagat ttctccactg aaataatttg
     7441 ctgatggagt atgtcggtaa tgcattccaa gtcgttagtg acagtgagtg ggcaaacgtt
     7501 caattgatct gctatttcgc gaatctgaat ggtccctttt tcgtgtaatt gctcacaaat
     7561 gtaaacttga cgaaaaatac ttttctccag ataggtttcc aacatagtac tcctcctagt
     7621 cattttatac ataactatta taactctacc aatcggaata g
//
LOCUS       DAZAID010000117         7549 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000117 DAZAID010000000
VERSION     DAZAID010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.120"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..1553
                     /locus_tag="ACG9BE_002565"
     CDS             162..1553
                     /locus_tag="ACG9BE_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3033271.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(1626..2273)
                     /locus_tag="ACG9BE_002566"
     CDS             complement(1626..2273)
                     /locus_tag="ACG9BE_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3033272.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2281..2967)
                     /locus_tag="ACG9BE_002567"
     CDS             complement(2281..2967)
                     /locus_tag="ACG9BE_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3033273.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2973..4046)
                     /locus_tag="ACG9BE_002568"
     CDS             complement(2973..4046)
                     /locus_tag="ACG9BE_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3033274.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4268..4942
                     /locus_tag="ACG9BE_002569"
     CDS             4268..4942
                     /locus_tag="ACG9BE_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3033275.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4947..6287
                     /locus_tag="ACG9BE_002570"
     CDS             4947..6287
                     /locus_tag="ACG9BE_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN3033276.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6436..7422)
                     /locus_tag="ACG9BE_002571"
     CDS             complement(6436..7422)
                     /locus_tag="ACG9BE_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFN3033277.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2347 a         1390 c         1328 g         2484 t
ORIGIN      
        1 taattattat acgaagggcg ctaaagggaa tcacaccaat tttcatacca attggtttca
       61 tttatgaagt tgattccttc gattaatgag cgtttcattg aaagaaaagg gttgagggag
      121 aaaaacatat gatcaggttg ttggaaggaa actatagaaa gatggaaaaa atcattcgaa
      181 ttctgtccat ttctgacaag tggatgacta aaaaagaact tgccaaacga attggtagtt
      241 cagaatctac atttgtaagg tatatcgaag agatcaaaat ccgctgggga acggatgtaa
      301 cgattcaatc ttcacaaaaa ttggggtatc gtcttgaaca tttgaatgta ccagcttatt
      361 taaatgttct gatggaaatg tcgcaaagtt ctacaaacag tcgtttatta aatgagatta
      421 ttgtcaatcc gggtaaaacg attgagtatt attgtgaaaa aatttccatc agccggtctt
      481 cttttacacg taagttgaaa caatgcaatc aaattttaga aagctattta ttaaaagtga
      541 tcgttgacca aggttttcag ttaactagcc ttaaaagtga gctccagttg cgtatctttg
      601 ttaccttttt ctttttaagt tactacggaa gacgcacgtt gccatttcca atcgaaaaaa
      661 agaagatcaa agaaattatg gtcaagaata attgtcagat gaatttgttt caggaagaaa
      721 gcccatatga gcaatctttc tttattatgt attttgttgt gcatctcatg agagaatccc
      781 aaggaactcc atttgaatgg aaagctggag actcttatgt acagttgata aatatcgata
      841 aaaaagactt cttaatcttg aaaaatctct taggaaaatt gaagtacagc agctatataa
      901 aagtagtgaa ctattttgtc aatacgattt tttcagaatt ttcggagaaa gaaaaaaatg
      961 aaatcagtga gcagatacga aaaaatatgt cctactgttc gttttcatcc gctgattgca
     1021 aagaaaaaaa cgaagaatat gttgttcaat tattaacgca aatttatttt ttctcccgta
     1081 ttttcccata cgatacgact catttgacga tccgtgcaac ttactttgca cagcagatgc
     1141 aacaccgcca gatacgtcta tatgctggat ttcgtgcaga acttcaattt tattccactc
     1201 tactgaatca gaatctgatt tattatttat ctagctatat cttttggatg cttatcggta
     1261 atcctgaaat tcaccattat acttctgata aaaaaatact gattatcagc gacttaagtc
     1321 ttcgacatag tcaatatatt gaagaatata tcagtgatat tcttgctgtg cataaaattc
     1381 actctgagac aactgctatt actgaagatc agctgtcgaa atacaatctt ctcgagtatg
     1441 atctgatcgt tacgaatcaa cccattttga atgcccatgt tcctcatata ttgattgatg
     1501 atagtgtgtc attcgcaaat gaagaagaac tcatccgctt attcgagtta tgaataatca
     1561 aaaaacagct atgtataaac caccctgata aaaatttcag gacaaataca catagctgtt
     1621 tctttttatt gtattccttc agcaatccaa cgagatttat agcgaaagtc ttttaacaac
     1681 tcttcttttg tccaattatt tgcaatgaga tccgtcaaag tgttcattac gagggtcatg
     1741 gaaaagtata ggaaactaac tgcttcttcc gacgattgaa tggagaaatg attgattctt
     1801 aataattcca gccatttttc gttcattgga gaatcagccg atagttctct acgtttggtt
     1861 gtctgctgat catttcgctg ggttaacaag tataaccttt gccaatagat actttcgtga
     1921 tctaagtcgc agcaccactc taaatatttt tcgatcccca aaaagaaatg ttgtttgttt
     1981 tcgctaatga attttaagat atcttgatgg actatgtgag aactgctttt gatcgtatac
     2041 aaataagcat cttccaaatc ttgaaaatat ttataaaaag caccgcgcga catccccatc
     2101 ttgtcaacga tttctgagac tttgacttgg ctgatgtgct gtgagcaaaa tatctccagt
     2161 aataggtcat cgattctctg tttctttgcg ttcgataaat tgaaaaaagt ttcttttggc
     2221 atccacatct ctcctttatc tctgattata cgtggatagc ccttcattgt catgaaggat
     2281 tcattccttc tttttctctt cttctgtcaa gtacccatct tccattcgat aaacgcgatc
     2341 actccattga atcatacgga catcatgagt aaccataata gtcgcttttt gtttctcatg
     2401 agcttcttta actagtaatt tgacgacttc atatgcatga tctgtatcca aactagctgt
     2461 tggttcgtct gctaagatca agcttggctc attatataaa gcgcgagcaa ttgccactct
     2521 ctgtctttct ccaccagaaa ggtctttagg ataactattt ttaagtgact ggatatctaa
     2581 agattgcagc aaggaatcga tttttgttga atcttctttt tttgaagtaa cttggtcaac
     2641 tagttcaaat tgttccttta ccgttaaaaa aggaatcaaa ttagagcttt ggagaataaa
     2701 tcctatttct ttaaagcgta aatctgctcg ttttttttct ggaagctcag ttagtgagtg
     2761 accgttgatc actatttctc cggaagtcgg tgtttgcaat cctcctgaaa tggtaaggaa
     2821 agtactttta cctgaaccag aaggaccaat gatgctgacg aattctcctt cctttacata
     2881 aaaatcaatt gctttcaaag ctgagacctt tgtatgacca gaaccaaatg ttttggtaac
     2941 ttttttcatc gttaatattt ttttcatttt tattaacctc ctattgctgt aattgggtct
     3001 actttggtta tcgtacgtat cgaaaacaat ccgcctagca ttgcgacaat gatcaaaatc
     3061 ccactatata gcaaccattg tgaccaaaaa acagcaaaag gcatcgcgct tggtaaaatc
     3121 agactagtca aataagcaaa caaaagagca agcagaacgc ctaccacccc aacaataaag
     3181 gactgtgcta ctaaagattt agcgatgaag aaatttttga tcccttgtgc tttcattact
     3241 ccaaaaatag cagtcttctg aagtgtcatc acgtacatga aaataccgac cacggcagca
     3301 accactaaga acaaaaagta tatcatcgca tttaaggtca tattttgggc agaatagcca
     3361 ggaatacttt caatgaatgt cccaatagtt aataactgta gacccttcga tgctttttct
     3421 ttcgaaattt tagctgaatc ctttattacg atcccattga tcggctgttc ttcttcagaa
     3481 gcaaacggct gttgtccata cttcaaagca gtccacgtat ctagggacgc atagatgacg
     3541 ggagaaactg tgtaataagt ttcaggaaag ataccgacaa tcgtcaaagg atcgtcgtaa
     3601 ctgccaattt tgatcgtatc tccaatttta tagcctgaat cagccagatt ttgagagatg
     3661 atgatttcat tctttgtttt aaattcatgc cctttcgtga gttttggtaa taaaaatgct
     3721 tctttcgttg tgccgaaaac tgtaacattt tctttgtttt ttcctttgat tgctccgctg
     3781 tacaatccga ttggtgcttt ctctcctcct tcaatgtatt tcagatctcc tcttgttaat
     3841 tgtgaagctg caaatacttg atttgcatcc tccgacaaaa caatctcttg tgcgtcccaa
     3901 tcatcgattg cttttttaaa ctcttctgaa agtccattcg ctagtcctga caacatgaat
     3961 acgacatacg caatcaagaa cataataccg acgatcaagc catatcgtaa tttagaatag
     4021 cgaatttctt tgatagctag aaacatttgt ttttcctccc tatgatagat tgatagtgac
     4081 aaaatgtcac ttgttgaata gtgtaactct aaagtgacaa tttgtcactt gtgttgtttt
     4141 tttcttatca ataaattgtc taagacatct ttcgataaaa ccttaacatg agtcataaaa
     4201 aatttattta agccttattt atgctttcca tgataaacta aagtgaccaa atcaatgagg
     4261 tgggaaaatg attaaactat tagtagtaga agacgataaa gtattatcca ataatataaa
     4321 agaaatactc gctgcaatcg gcgagataac acaagtttat gatggtagtg aaggactgtt
     4381 cgagttggaa agtggagttt atgatcttgc aatcttggat ttgatgatgc cggaaatgaa
     4441 tggatatgaa gtgttagcaa aaatacggaa agaaaaaata cagactccag tattgatttt
     4501 gactgcaaaa gatagtttgg aagataagat tgaaggattt gaacgaggtg cagatgatta
     4561 tctaacaaaa cctttctatc gagaagaact gatcatgcgc gtgaaagcat tattgaaacg
     4621 ctcgctaggt ctctttgatg aaaatgttct gaagtataag gatatcagtt gtgatttaaa
     4681 aagcaatgaa gtcttgtata aagaacaagt tttgcccata caaggaaaag agtttgaatt
     4741 gctcgtttat tttttacaaa ataagggaat cattttgaca aaagagcaga tatttgaccg
     4801 gatctgggga tttgattctg atacgacgat tacagttatt gaagtttata tgagcaactt
     4861 acgaaaacat ctgcgaagta ctggattgga taaagaaatc aaaaccttac gtaatgtcgg
     4921 ttatatactg caaagggtgt gatgcaatga atggagatat tacacgaaag caacgtagtc
     4981 gcttttttgc gacaaatgta gcagcatttg cttttatctt ttttcttttg ggattcatta
     5041 ctttacagat attaaaccaa tctgcataca gagaaacaga tctgtcatta gagaaaataa
     5101 attttgattc tcgaacagta caattagaga tacaaagata tcagcaaggc gatccctttt
     5161 taaacccgac tcaaacggaa caaaacagaa caaacatgga acagccatta atggaaccat
     5221 caaatgaccg ttttaacact caagtgatct tgtggtctaa aaatggtgag atattgaata
     5281 aagcagctat tggtgggcgt ttcaaccaaa tcaaagaatt aaaaatggat acgaaaaatc
     5341 tgaataccat ccaaaatatt gaattaaatg aagacagtgg ctcatctgcc ctctctttcc
     5401 gttcgattac gaaaaaagcg gagacgaatc aagatggcgt tgcctacatc caaattttag
     5461 aaaatacaaa tcaaatcaag aattcattag cgacttttcg cacgattatg attttgtgta
     5521 tgattgtttt ttggattctc tctattggta tcagttatta cttgtctagt ctaagcatgc
     5581 gccctattat tctttcttgg agaagacaaa aagaatttgt tgagaatgct tctcatgaat
     5641 tgcgcacacc gttaacgatt attcaaaata gtttggaaca tctttttacc aagcctaatc
     5701 atactattat tgaagaatcc gaaagcatcg ctcaagcatt gtcagagacg aggcgcctca
     5761 ctggattgac ttcggatttg ctgacaatcg ctcggaatga ttctgatcag cagctgcttt
     5821 caaagcaaat gatcaataca caagaatata tcgaacaact tgttaaacca tttcaagaga
     5881 tggccatcat agatggaaaa gagtttattc tccataacga tgcaacatta gaagtaccaa
     5941 ttgatgaaaa aaaaatccat caagttttgg ttattttgct ggataatgca ttaaaatata
     6001 cgaatccaga agataagatc attttggatt catctgttaa ccataaagaa tggctgattc
     6061 acgtaaagaa tactggccct tctatcagtg aagaagatca agcaaggatt tttgatcgat
     6121 tttatcggga agatcaatct cgggcgaaag agactggcgg atacgggctt ggcttggcaa
     6181 ttgcaaaaca ggtaatcgaa cagcatcatg gacagatttc tgtatccaat tatcttccac
     6241 aaggagtcga ttttaaagtc caattgccgt tgaagcctaa aaaataatgc ttatccctaa
     6301 aaaagggctg tgacaaaaat cttgtcacag cccttttttg ttgcgaataa acggtgttcc
     6361 agaagtagct tcttcggaaa taagccgaaa tttctttaag ctgaccactt ctgtcacaac
     6421 ctctttagct tacgcttacg attttttatt gcggtcttca atgtgtacat caccgcttgt
     6481 gtcaatatcc aattcttctc ggctaacatc ttctgtcact ttttcaacat cttcatgttc
     6541 ttctttatga atcccaactt cttcacgaat aactgtatgt ttgcttacat ctacttgctc
     6601 ttctttgatt gggatagtaa tcgtgtcatc ttccaatttt tcgtctgttc cctgtgaaga
     6661 ttgatctgtt actggtttgc gttcgatgac gatttcttca cgtttgaccg gtacttctac
     6721 cgtttcagta tcgttgacca catgtttgtg gatgtcgact tctccagttg tcacatcatg
     6781 tgtatttaca tctaattttt cttcttttag ttgaattgta tcatcatcta gatttggttt
     6841 gcgatcgatg tcttcagtct tgcttgtacc cattgtatca gtctcgccag gtacgtccat
     6901 cggatcagtt cctggtgtca tattcaatgg atccgttgta ttttgaaagc ctgtcgtgcc
     6961 acctacgtcc attggatctg ttccagtgcc agtttctaat ccagtcccca ttgtgcttgc
     7021 tgggctaaca gtatcttgtg tatccattgt atcttctgtt gtttctggct gacgatagcc
     7081 ttttactgtg atgacaaatt ttccatcaga aatatctcct ctatattgat ataaaggatc
     7141 attttctggt gtagccgtct cagaatcata ggtatcaaaa gaaaaagtat cttttatttt
     7201 ttcccataaa gaacggtctt cttcaccatc catccgattt tcatctgttt caacatctat
     7261 accagacaat ccgtcaaatc ctagtctaga tgctgcttca tcagtagtat aaataacgat
     7321 atcatctcgt tcataccctt cgttacgcaa ccgttcaatt actgataatg cttcttccct
     7381 cgtactatag cttccgatga ccgttaaatt tttctcttcc atgtaaattc ctcctagcaa
     7441 atagatttat gattggtata ttgttactta taaatttatt ctataggaga cgattgtttt
     7501 ctacaaatat ttagattgga atttgattaa aacaatttaa ttgaaattt
//
LOCUS       DAZAID010000118         7500 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000118 DAZAID010000000
VERSION     DAZAID010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.121"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..328)
                     /locus_tag="ACG9BE_002572"
     CDS             complement(38..328)
                     /locus_tag="ACG9BE_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033278.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(446..706)
                     /locus_tag="ACG9BE_002573"
     CDS             complement(446..706)
                     /locus_tag="ACG9BE_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033279.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(843..1256)
                     /locus_tag="ACG9BE_002574"
     CDS             complement(843..1256)
                     /locus_tag="ACG9BE_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFN3033280.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1712..2197)
                     /locus_tag="ACG9BE_002575"
     CDS             complement(1712..2197)
                     /locus_tag="ACG9BE_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033281.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2336..2551)
                     /locus_tag="ACG9BE_002576"
     CDS             complement(2336..2551)
                     /locus_tag="ACG9BE_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFN3033282.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2775..3575
                     /locus_tag="ACG9BE_002577"
     CDS             2775..3575
                     /locus_tag="ACG9BE_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN3033283.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3594..5462
                     /locus_tag="ACG9BE_002578"
     CDS             3594..5462
                     /locus_tag="ACG9BE_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFN3033284.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5455..5946
                     /locus_tag="ACG9BE_002579"
     CDS             5455..5946
                     /locus_tag="ACG9BE_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFN3033285.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5934..6488
                     /locus_tag="ACG9BE_002580"
     CDS             5934..6488
                     /locus_tag="ACG9BE_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFN3033286.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6506..>7500
                     /locus_tag="ACG9BE_002581"
     CDS             6506..>7500
                     /locus_tag="ACG9BE_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFN3033287.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2666 a         1202 c         1431 g         2201 t
ORIGIN      
        1 ggaagttggg acaaacgagt taatacattt taatacatta ttttttattc aaaaaaaata
       61 gcacaatggc aaagatcgta agagagatca taagccccaa tgaatgacta aaaagaaaag
      121 cagaatcaca tgccatatca tcccatattt cttcaagaaa ataaataagc gggaaaaagg
      181 gaaggaatag tagaacatcg atccagcacc atttttttct tcttttgaaa taacataaaa
      241 caataaacat gaacgtgcag ggaagtaaaa acaaagcttt actcataact aacaaaatac
      301 cagctccttt tatttttttc tcccccacat tcaaagagta acaatggtat gtacttaata
      361 atagaaaaat ggatcaactt gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa
      421 cttccagcca aaactgactg gaagtttatt tttttctgaa atattctaac acaaatatga
      481 aaaagatggc cgaagctaat aagccgattt cgtagcaaaa caatgagata ccagagtcgt
      541 aataataaaa tcctctaatc ttatggataa aatagatcat cggacaagca gataagagta
      601 agtatttgtt aggccttttg aataagtaaa agaaagcact ggtcattatg ataaatggta
      661 tccaaaacaa aagtttactt aagacaatca aatgatcaac tcccatattt tttaccaaga
      721 gagatcataa catctttatc gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa
      781 acatactcgt tcttatccaa atatataaaa atcgcccctc attcatcgag aggcgataag
      841 ttctaattta actcaatttt ttttgtttct tttggctgat aagctgtttt aggtaatacc
      901 aatttcagca caccgttttt aaaggttcca gtgatttttt cttcatcaac attaggaaga
      961 tagaatgaac gtttgtaaga gaccgaatgt ctttctttcc gtacatagtt gccatcttct
     1021 ttatcttctg tatgggactc atgattggcg gagatcgtca aagtattatc tgaatattca
     1081 actgtcgtgt cttctttgtc acagccaggc atatcagcag ttagtttgta ttctttttca
     1141 ttttcgacta aatctacttt tggataactt gaaacaccta aaaaatcatt gaatgctgga
     1201 ctaaacacgt cattgaaatc agggaacatg tcgcgaatgc taggtacatt tgccattttt
     1261 gatcatcctt tcaataagta ttttttattt atccctattt acgtaataac tctattttga
     1321 aaaaaatgca agtataatgg ttagctttgt gaatatttca tctaatacga tacaaaggct
     1381 atctttctaa tttgaaaaag ggatcattaa aataataaga agaaaatatg tcaaaaggta
     1441 aaaaagtaaa ttcttctatc caaatataga aaaattgaat ctagaaaacc gcccgacaat
     1501 aaatttcatt aactcagttg accgaaaatg aagtagatac caaaaattta tcaagagagc
     1561 aagtagagaa gtgagtatat tcataagaga aatagctgta acgaattatt aggttaatgc
     1621 aaatgcttat ttatactccg tataactttc tcagaaaaaa tgataccaat aatgacttgc
     1681 tagtactgta aatccgtata atatcatcta atcaaaatgg tgttccattt gcatctgtca
     1741 tcgtaatcga tccttcgggt gacactcgat aaaaaccaac gcttccagat cctccttggg
     1801 aacggatttg tttgctggaa gctgtgaaca aataatctcg atcaattttt tgataaaagc
     1861 taataacaat atcgtcactt tgtaattcgg aacggtgggc aataagaaat agaagtgctt
     1921 tgtcagagtt ttctaatgga agagaaggta cagtgctttc ttgcatttca gatgttgaag
     1981 aaatgctaaa cgactgaata cttgattggc tatgttgcga atctacatta ccagtagaag
     2041 tattagtaat ggaagtagta gaagtacttt tagttgaacc aacctcagtt gttgaaatgg
     2101 ctgctgaaga ttctttaaca gaattctttg ttttatatcc acaggcagaa aaaagtatta
     2161 cagttcccaa caagcctgcg taaatccctt tatgcatgat agctcctcct aaaatagatt
     2221 atctttattc agtatatcac ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct
     2281 tcccaataaa gggaaggttc atgaagataa taactttttt aataattata gctttctaat
     2341 atctttcatc aacttttcta gtaatcattg ggaaaaattt caagcagtag atcgcacaaa
     2401 tacctacgac gatataaatg atcttcgcaa aaatagttgt tgaaccacca gcaatcatag
     2461 ctactaaatc aagttcgaac aagcctacca acagccagtt caaccctccc acaatcaata
     2521 aagttaatgc aatagcatct aaagttttca ttagaattct cctttcttaa atgataaaac
     2581 aattatatat caaaaagaga aacattgctt atataacgct caggaatttt agcaatctat
     2641 gcaaaaagat gaaaattttt tttgctcttc cattagtaca cattttttag ctgaagctgt
     2701 aaatttcatg tcttaatttt ttttagtgag cttttatact ggatttgtaa gaaaaaagaa
     2761 ggagaggatc acatatgtcg gattggttgg atattgcagg aaaagtagta attgtaactg
     2821 gaggttcctc aggaattgga agaagtatcg tcgaaaattt gttgaagcag aatgcacaag
     2881 tagcgaattt tgatgtaact gaatgccgta tacagcatga aaacctacta tctttaaaag
     2941 tcgatgtgtc ttctaaaacg gacatagaag aagggatata taaagtaatg aaacatttca
     3001 agacgatcga tggtctagtc aataatgcag gaataaatat tccttctctt ctaatagata
     3061 gaaatcatcc aaaaagcaag tatgaattat cagaacaagt ttttgataag atgatcgctg
     3121 tcaatcaaaa aagtgtatat ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg
     3181 gatcaggagt gatcgtgaat ctttcttctg aatcaggatt ggaaggatca gaaggacaga
     3241 gctgttatgc tgctacaaaa gcagcgatga acagttttac tcgttcttgg gcaaaagagt
     3301 taggaaagag gaatgttcgt gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt
     3361 tgagaacaca agaatatgaa gaagctcttt cttatacgag aggtataagc gtcgaacagc
     3421 tgagaaacgg ttacagtcga acaagtacga tccctctagg aaggagtggt aagctgcaag
     3481 aagtggcgga tctagtctgc tattatcttt cagaaagatc aagttatatc actggagtca
     3541 cgactaatat ttcaggaggt aaaacaagag gataaaggat aggggggacg cacgtgacgc
     3601 tagtaaacag atggtaccaa ttattgaaat tactagtcac tcacaagaaa gtttcaatcc
     3661 aggaagtgca aaaagaaatc aaaaccagta atcaaaccat aaaaaaagat attggggagt
     3721 tgaatgaaga aattgccggt attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg
     3781 aaatttatga ttttgatttt tttgatgaga tcatgcaagg aaaattgaaa acagccagtg
     3841 atttcaattc ggcatccaaa agaatcgctt atttactaaa taggctgatc gaaacagagg
     3901 atttcttacg tatggatgat ttgtctgaag aagtcggtgt cagcagagga acagtggtca
     3961 aggatctaaa gacattaaaa gagatgatca atgattttga tgtaaagatt acaggcacac
     4021 caaataaagg actaagaata ataggagatg agctagaatt acgactactt ttattttatt
     4081 ttgtttctcc ctacttttca gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa
     4141 tcaaacattt tcagaagaag acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag
     4201 ttatcggtat ttctttgaat agaatctcta gtaactatct gttgaagaag cccatcaatc
     4261 gttacagtgg gtgttttgaa tatatccaag aattcaatga actgatcaat cagttggaag
     4321 aaatctatga actaactttg agccaatatg aaaaagattt tatcagtttt cctttgaaca
     4381 tcatcaacac aagtataacg atgataagga aagctaactc tgatttgcgc ttatatttcg
     4441 accccatgat gatgcgtatt cgacgattag atctcattga tctggatgag tccttcttat
     4501 atcaagagat gaaacagcac ctctctttat tgataaatcg ggtaattttt cgttatcgat
     4561 tgactgattt attttatggt gagatagaaa aaaagtatcc tttttcttac caaatcgcta
     4621 gcgaatgcat acaagcatta gaagctatct tgcaacataa gatctctaaa gtagagatca
     4681 gctacctaac gttgtatttg gagttagcat ttcgatatca aaaagagcat ttttcctcta
     4741 aaaaagtagc cattgttcat acgacaggaa aaggaacagc attaatgatc caaagacaaa
     4801 tcaaaaggat attggggagg gatatacaaa taacacatgt ttcagaagaa caatatgaaa
     4861 aagtagatct aaacaaatat ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg
     4921 aagagacacc ggtgatccat ttgactaatc tttttgatga ctcttggtta agggcacaat
     4981 ggcaaaaagc aaaaaaaaag cgattagccg ggtttcattc tactgttttt ttctatcaaa
     5041 tatggaaaga aaaaaagacg tatcaagaat atttaaacaa attagtcgct cctttgatca
     5101 gaggctatta tgtggatgaa gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa
     5161 gcacatttgt taacaaagaa tttgcttttc ctcacacaac caaccagcta tctcaacaca
     5221 ttgttttatc agtagggcta ataactgatc aaggtttagc agaaacagga ggaaatggag
     5281 ttattttttt atttgccata cctgaagaaa tgacggctga agttgagaat gaattatttg
     5341 agctatacga tatcctattt gaactcataa aaatgggaat agatgagttt attgaattag
     5401 caccgacaca agaatcattt ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat
     5461 gatatgtatc tattcatagt tttcgcagct tcagcttatg tgctgcaagc agttatcagt
     5521 tggaagcaac taaagcattt caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta
     5581 gcgattggta aacgacctgg gaaaatccgt tcaggcacga ttgttatgct agctatagac
     5641 gatcatgatc aagttttaga agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt
     5701 cattcattac ctgtctatgc gggtgaagat atccattaca tcgatcgtta ccatccctca
     5761 gttcgagcgg aagatcgttt gacacaagca gctttagaaa atgccagaga agtatactta
     5821 cgaatagagg caggaaatga aaaaagtgaa caaccttcat tgccttttat tgctatgctc
     5881 aataattttc ggatatggaa aagtacgatc aaagaaaaaa ggagtgacaa acgatggata
     5941 tcataacaaa atttgctgag ggatttatga ggctgttcca aacaggtgca gaaacattca
     6001 taagctggat gacaggaatc gtaccagttg tcttgatgct gatggttgca atgaatacgc
     6061 tgattgcttt tttaggtgaa gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc
     6121 cgcttactcg ttaccttgtg ttaccgttct tatctgcttt tatgttggga aatcctatgt
     6181 catttacaat ggctcgtttc cttccagaat actacaaacc aagctattat gctgctcaag
     6241 ctcaattttg tcatacgagc aatggtgtat tcccccatat taatcctgga gaattgtttg
     6301 tatggatggg gattgctcaa gggatcgaaa ccttagggct aaattcgatg gagctggcta
     6361 tccgatactt gcttgtggga ctgctgatga actttattgg tggttggata actgatttta
     6421 ctactggatt tgtatgcaga cagcaaggaa tcgttcttag caaaaaagta gagctttcag
     6481 ttgattagag aaaggagagt tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg
     6541 cgggtatgga ggtcctttga ttattaggcc gacaaaagat aaacacaagt tcatctatgt
     6601 cacaggtgga ggtgagaggc caatgatcgt agataaaata gaagaactga caggtatgga
     6661 gtctatcaat gggttcaaaa catcaatacc tgatagtgaa atcgcgcttg caatcgttga
     6721 ctgtggaggg actcttagat gtggaattta tcctaaaaaa gggataccaa ccatcaatat
     6781 cgtttccaca ggaaaaagtg gcccattagc acaatatatc actgaagata tttatgtttc
     6841 agcagtaggg ttagaacaaa tacagccact aatggaaact gatgataaca aagaaacagt
     6901 aaatactgca tctccagata aaaagtacaa atatgatact tctaaaaaga taaccgagca
     6961 aaaagcagag cagcaaaact ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc
     7021 tacattcaat caatcagcaa gagaagcagt agatacgatg atccggacag tgatcccttt
     7081 tatggctttc gtttcccttt tgattggtgt gatacaaggt tctgggatcg gtaatgtatt
     7141 tgctaacatt atggcacctt tagcaggaaa tgcagttgga ttaacaatca ttggctttat
     7201 ttgttcactg ccatttttat ctcctttgct tggtccaggg gcagttatta gccaagtgat
     7261 tggaacattg atcggtgtag agattgggaa aggaaatatt cctccccaat tagcattacc
     7321 ggcactattt gctatcaata ctcaaaatgc atgtgatttt attccagtcg gtctaggttt
     7381 ggaagaagca aaagctgaga cagtagaagt aggtgtacca tcagttcttt attcaagatt
     7441 tcttaacggc atcccacgag ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAZAID010000119         7296 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000119 DAZAID010000000
VERSION     DAZAID010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.122"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..629
                     /locus_tag="ACG9BE_002582"
     CDS             <1..629
                     /locus_tag="ACG9BE_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033288.1"
                     /translation="KFANSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLL
                     VNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENS
                     QWIKELKREDFIETLRILKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEK
                     RIDNIINYINSLSEPLKCRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
     gene            812..3487
                     /locus_tag="ACG9BE_002583"
     CDS             812..3487
                     /locus_tag="ACG9BE_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFN3033289.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3669..3908
                     /locus_tag="ACG9BE_002584"
     CDS             3669..3908
                     /locus_tag="ACG9BE_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033290.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3924..5297
                     /locus_tag="ACG9BE_002585"
     CDS             3924..5297
                     /locus_tag="ACG9BE_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3033291.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            5301..6935
                     /locus_tag="ACG9BE_002586"
     CDS             5301..6935
                     /locus_tag="ACG9BE_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN3033292.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            7098..>7296
                     /locus_tag="ACG9BE_002587"
     CDS             7098..>7296
                     /locus_tag="ACG9BE_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033293.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2602 a         1137 c         1449 g         2108 t
ORIGIN      
        1 ctaaatttgc aaactcattc tggtttttaa aaaatattaa taaaacagtg gaaaagaatg
       61 gagtttataa aggtgatttt cattttcaaa atgctgagac tattgaacga gaacatcatt
      121 atttcgattt attagtgaat acagtggtag aattagcgaa atgggcaaag tcaaacgaaa
      181 cttatagtca agtacttaaa agaattatga gtcaagaaac ttcatttatc tatgagttaa
      241 gcaagtcaaa tttgaaatat acttataatg aagatgaact tgaaatttta gataatccag
      301 aaaattcaca atggattaaa gagttgaaga gagaagactt tattgagaca ctaagaattc
      361 taaaatcgtt atttgatttg aaattagcca aagctaccat catgggaatt gaaagagatt
      421 catattattg ggaggaaatt ggactatatg ctccccagat tgaaggtgaa gataaagaga
      481 aaaggataga caacattatt aactatatta atagtttaag tgaaccttta aaatgtagag
      541 gacttgattt tataggaaca ttaggatgga cagaaacata ctatgagaaa atatataaag
      601 aaataattga acaatatttt cctaaataat ttaataagga ttgatttatc gtaaaagaaa
      661 aaagttgtga tttccaaaat cacaactttt ttcttttgtc aagattttga gtgggatata
      721 tttttgtaga actgaagatc atgaaattat gagaggttgg aggtaatgta gtcatacaaa
      781 taaaaatttt ttagaaagtg agtggtaatt aatgaaaatg aatcacactt caattcagaa
      841 gcatttacaa ctgttaaata cactggggat ttcttatgtt aatttgagaa ctttttctac
      901 attaaacaag aaaaccgttt ctcatatttt ttctactgca gattctcaag caattattaa
      961 ctatttagaa aaacagaaat tgtctgaagg tacagtaaac ataacttata atcctattaa
     1021 acaagaggtg attacacaaa aaaagcattc tgtgagagac aatgatattg aaaaaattcg
     1081 gtttgtattt attgatattg accctaaaag gaaagaggga agtgcaacag atagcgaaaa
     1141 gaaaaaggca gaaaatgtta tggaacaagt agaacgttat ttgaaagaaa agagaataga
     1201 atcatttgta aaagttgata gtgggaatgg ctaccatatt tttcttccca taaatgaaca
     1261 accaaataat cacgagacga ttcttactat tcagaatttt cttcaattac ttcaccgaag
     1321 atttggagta gaagatgaag ttgatattga tacttctgtt tataatcctt caagattatg
     1381 caaattagtt ggtacaccag cggtcaaagg aaagtccaca aaagagcgtc cgcatcgttt
     1441 atctagtttc ctttctattc ccgataacat agatagaagt aattgtttta aaattcttga
     1501 acagatagtt gaagaaaatt ccatgatgtc aacctctaga gtaaaaagcc atccatcttt
     1561 tagagaagtg ttacctttta ttcaagcaga ctataaacaa tggttagctc actatcctga
     1621 attaacttat accataaaag agggcgatca taaaggagtt accttactta tatttgacga
     1681 atgtccatta agaaaacaca cgaataattc taatggttct tgtttgtctg ttacggctga
     1741 taataagatt cgatttcatt gtctgcatgc ttcggataaa aataaagata ttcaagactt
     1801 tgtaaaaaaa tatccgttgc caaaacctgt aaatccttct gtagaatcat tagaatccaa
     1861 tcaagaatat tattttgacc agtttaaact gaatcaagaa ggagtatttt tggtgaaaga
     1921 aggtcaagaa gaaaaagttg cttgtccgat gtttattaag caaatacgca aaaatatcga
     1981 acaaaaagtc attacacttg ttcttgccta ttatgtcgat cattgttggg atgaaataga
     2041 aatagaaggt ggatgtttac aaactaataa ttttaaacaa ttagcaaaac atggcttaat
     2101 cattaaagct agaagagaag ctgaagtcgt tgacttttta cagattcaac gggagaatgc
     2161 tccaatcttt tttgaacatg aatctcttgg ctggaaaaaa gaaaatgaag caatgatttt
     2221 tcagttacaa gattcttttg gacgtgaaat tccttctgta ttaactaaaa actcatgcta
     2281 ccaattaaca actaatggta cagaagcgga atttgacgca ttggttcata agtatgtttt
     2341 aggaacgaac ttagaacttg cttggtcgat tggtattgct tctattgtgt taggctattt
     2401 aaacgttacg aaggctaata cttttttgag tttactaatt aacctacagg gcaagagcac
     2461 aacgggtaaa accactacgt tacactttat tgcctcttta tttggaaatc caactacggt
     2521 tcttagaaat atgaatgcta ccaataaagc cattataaag ctagcatcaa acaatcaggg
     2581 aggtgttcca ctaatactag atgagttagg agcagcaact caaaataact ttagctcgtt
     2641 gatctatcaa ttagcttcag gagaagaaag gctaagatta gattctaatt gtcagcttat
     2701 gccacaagag actttttctg ttttgaccct tacctcttct gaagagcgtt tggaaaatta
     2761 tttcaatgag aaattaactg gtttgaaagt tcgatatatc gaattttctg atatcaagtt
     2821 tacaaaggat gcgaatcacg cagagaaagt taaaagtctt tcttctaata cttttggttg
     2881 gggtataaag ttatttcttt tgaaactctt tgaacaaggg attacaaaaa ttgaagcaac
     2941 ttttgtcgaa gctcaaaaaa aactatgggc tgttttacct gaacattctt tgaagcaacg
     3001 cattggtaat aatctagctt tgatttatag tggagccctc ctaattcaag atatactggg
     3061 ctatccttta cgattagagc atatacagac ggtgcttatt aattcgtacg aacagttaat
     3121 tgtagatact agtttaggaa gtgagcaaac ggtatttgac cgagtgaaaa cattgttggt
     3181 gatgaatagc cataaatttt taacaaacag tgacaacaaa catacgccca gtacgatttg
     3241 gggaaaagta tcaattcaaa aaaatgggga tattaaggta aatatttttc ctatcccgtt
     3301 tgaggacttg ttaaggcgag aatttcaagt aaaggatcta acctatcttt ttaggcaatt
     3361 gcttgattcg ggtaatatgt gtgcagaaaa agggagaaga acgaaacgta ttcacatgaa
     3421 tcgtcaagtt cttccaacat acgagattct attaccttct tcactcaaat cgtattttcg
     3481 attgtaattt agaataaacc aaagtatgaa agtgaggcac catgttatgg aaatatttct
     3541 tattgattta gcaaaacaac tgattactta tctgtatgat agcgagcaaa cccaaacgga
     3601 agtaaatgaa gcttgatcta tgcttcattt acttcattca atcaaacatg aaagaaagga
     3661 aaattctaat gactaaaaat aaaatgacac taaaagcaga ggttcttctg tatattcaag
     3721 aacatttttc aaatcaagcc ttttttacaa aaccaattta cttggatttc gaaataagag
     3781 gggtatctgc tggatctatc gggggtactc ttcaagcgtt gaagaacgaa ggatatttag
     3841 aaaatcactt tgtgcaacgt tcgttcaata gaagagtcgt gaaaaaatgg tatttagttc
     3901 attcatagag aggggaatca agcatgcgtg tggcttgtta tgatcgaacg tctgtttatg
     3961 aagaagcgaa aattagtaat gacgctcaat atgatgcagt gaaagagtat gttttagaga
     4021 aatatgaaca aattccccaa cgattttcag acaaaggaga atcaggggaa aaagcggagc
     4081 gtccagcttt tcgacagctt agagcttggg tttgtgcgtc agaagaagag ggggtgatct
     4141 atgtttggcg ttatgatcga ttggcacgtc aagccaaaat agccttagaa tttattgagt
     4201 tgtgccaaca acatcaggtt caagtgtatt cgatttctga acctcttcct gcttctttct
     4261 cacacgcgtc aacgaataaa ctatttgtgc aattattgtt cacttttgca gaacaacaac
     4321 gaaacgtcat tattgagaat gttcgtgccg gactttccta taaaaagcaa caacggcaat
     4381 atttgtcttc tgcggtacca tatgggtatc gtttagtgaa tgggaaaatc caacaagaag
     4441 tacacgaagc aaggattgtt cgctatattt ttcaacttta tttgaccaaa aaatatggct
     4501 acaaaaagct ttgtcaacga ttgacgcaac aaaagctttt cttccgcgaa cgtccttttc
     4561 agccctatca tatttatagt atcctaaaaa atccattgta ctatggcgaa atcaaaggag
     4621 gatctttggg gaagtatcta ggaacctttg aaccgattct ctcgaaagca acctttttac
     4681 aagcccaaga gattcgccaa tcaaggtgta cagctaaaaa agatacgtat ctgtacttat
     4741 tacgtcaaaa aattcggtgt cccttctgtg gtcgtcatct gagttcgaag tatcaatgga
     4801 atacaaagaa gacgaagacg cttcattatt atcattgtac cgaccgaagc tgccaaggaa
     4861 tttttcttcg ggcagaaagg gttgagtcag aagtattgga aaagatacag gctttttttc
     4921 aatacgaagg cgtctatcaa gccttgattg cccaattaac gcaacaatta gccgaaaagc
     4981 gaaaagatga aaaacgcaaa gtaatccaac aaaagaaaca agaagcacaa tggattaagg
     5041 catttgaaga gggcaattgt tcactggaag aactcaagca acaactgcaa gctcttcaaa
     5101 agccgatgtc tacttcttcg tttctttcac tccaacagtg tgaagcaaaa gtgaaagaat
     5161 ggcttcaact aaaagacctc tctagtcgac aattattatg tgactatgtt tgtcggattg
     5221 aggtaacaaa ggataaaaca gtttgtggga tttacttccg acaactgaca caaaatattt
     5281 tagaaaggaa cgaatgatct atggaaatga tacaaaccaa acgagtcgca ttgtatattc
     5341 gcgtgagtac acaagaacaa gctttagaag gacatagtat tcaagcacaa gaacaaaatg
     5401 gacgacagtt tgccgaacgg atgggctatg aagtggtaga agtttatgcc gatgaaggaa
     5461 tcagtgggaa atccacaaaa aatcggttcg cctatcaacg aatgatgaac gatgctcgga
     5521 accatcgttt tgatctacta gtgatttgga aattgacccg tctcggacgc aatatgttag
     5581 atgtcttaca aacagtcgag gaattattgc gatatgacgt aggattacat tcgatctcgg
     5641 aacaatttga cgtgaccact tcgacaggta aattgatgtt acaactcctt gggagtttcg
     5701 gtgaatttga acgcaaccaa attgcggaga atgttcaaat gtcaatgcaa agcttagtga
     5761 aagataaaaa gcgttatgca ggaggccgac gtttaggcta tgtcagtggt attgacacga
     5821 atggtcaaaa acaattggtc attgaaccag aagaggcgaa aattgttcgt ctgatctatg
     5881 caaaatattt aggcggagca gggtatcgag cgattgccaa ttttttgaat cgtcaaggat
     5941 atcaaacagt caaaaagaat gctttttcta ccacggcagt caaagatatc ttacgcaata
     6001 aaatttatgc agggtattta gaatatgcgc gctacatcaa ttgggaaacc aaacgacgga
     6061 aggggaagaa tccgagaccg attttagtga aaggaaccca tgaaccgatt atttcggaag
     6121 aagattatca aaaagtacaa gaacgccttg ctttagaagg aaaacaaccc aaatggaacc
     6181 atacaggtga aaatgtattg acaggcttgt tacgttgtcc agaatgtggc gcaccgatgg
     6241 cagcgagtaa tgtcacaaat accctaaagg atggcacaaa gaaacggatt cgatattatt
     6301 cttgttcagt ctttcggaat aaaggagcaa gtgtgtgtca tgccaattcg attcgtgccg
     6361 aacaagcgga aacatttgtc gccgaccgat taaaggagat cgttcagcta ccagaagttc
     6421 ttccccgatt ggtggcagcg ttaaatgaag aaatagtgcg acaaagccaa ccattggaac
     6481 aagaactcgt ggtgttactg gaacgaaagg aagaattgaa gactaaaatt gaaaaatggg
     6541 aagctgcttt ggaagatagt cctgaattgt ttccgatgtt aaaagaccgc ttagacgaat
     6601 tgactgaaaa acgacgacag ttgcacattc gagaaaatga aatcttaggg atctttcaac
     6661 aaggagagcc gattcaagtc aaagatgtcc aacggatatt gactagtttg gatcgttttc
     6721 ttgcccagag tgagaagaag caaatcaaag ccttgtatcg tacctttata gagaaaataa
     6781 cctttgatcc taaccataag gagatgttgg aaattacgat gaaattcacg catgccgtgg
     6841 tacaacaatt gaatgagcaa tatcaaatag ccgtatccaa aacaaaggat acggctattt
     6901 ttgtgttaaa aacacccttt acgttaacga tctagtctag gttatatcaa acaacgttag
     6961 aggggcgcta acgcttctct agcgatttga aatcgttaga gaagtagtta gggaggtcct
     7021 tatcataaat agggatattt attaatgaat atttgaaata taattgtata taagaattaa
     7081 aaagagtggg gatagatatg aaaaagatgg gtaagtgtat tttatgtgaa aaatacactg
     7141 aacttactaa agaacatgta ccacctaaaa aatcagggaa tactggaggt aaaaagacca
     7201 gaactggtaa tttagatgat ttccttaaga gcgattttac aaaaggtgat tttccgaagg
     7261 ggattaaaag gaaacctcaa ggaaatgttt attata
//
LOCUS       DAZAID010000120         6688 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000120 DAZAID010000000
VERSION     DAZAID010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.123"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            296..892
                     /locus_tag="ACG9BE_002588"
     CDS             296..892
                     /locus_tag="ACG9BE_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN3033294.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(950..1432)
                     /locus_tag="ACG9BE_002589"
     CDS             complement(950..1432)
                     /locus_tag="ACG9BE_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033295.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1538..1735)
                     /locus_tag="ACG9BE_002590"
     CDS             complement(1538..1735)
                     /locus_tag="ACG9BE_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033296.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2030..2788
                     /locus_tag="ACG9BE_002591"
     CDS             2030..2788
                     /locus_tag="ACG9BE_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFN3033297.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2848..3276
                     /locus_tag="ACG9BE_002592"
     CDS             2848..3276
                     /locus_tag="ACG9BE_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033298.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3322..3394
                     /locus_tag="ACG9BE_002593"
     tRNA            3322..3394
                     /locus_tag="ACG9BE_002593"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3355..3357,aa:Gln,seq:ctg)
     gene            complement(3544..3915)
                     /locus_tag="ACG9BE_002594"
     CDS             complement(3544..3915)
                     /locus_tag="ACG9BE_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN3033299.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4130..4315
                     /locus_tag="ACG9BE_002595"
     CDS             4130..4315
                     /locus_tag="ACG9BE_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033300.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4398..4586)
                     /locus_tag="ACG9BE_002596"
     CDS             complement(4398..4586)
                     /locus_tag="ACG9BE_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033301.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4610..4873)
                     /locus_tag="ACG9BE_002597"
     CDS             complement(4610..4873)
                     /locus_tag="ACG9BE_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033302.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            5031..5288
                     /locus_tag="ACG9BE_002598"
     CDS             5031..5288
                     /locus_tag="ACG9BE_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFN3033303.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5359..5550)
                     /locus_tag="ACG9BE_002599"
     CDS             complement(5359..5550)
                     /locus_tag="ACG9BE_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033304.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5691..6011)
                     /locus_tag="ACG9BE_002600"
     CDS             complement(5691..6011)
                     /locus_tag="ACG9BE_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033305.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(6034..6468)
                     /locus_tag="ACG9BE_002601"
     CDS             complement(6034..6468)
                     /locus_tag="ACG9BE_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN3033306.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2098 a         1302 c         1021 g         2267 t
ORIGIN      
        1 aattttctat aaaagcaagt aatttttaat ggcaatatca taaaaaaaag aagatagaga
       61 tttaataatt atatatcagc tgtttttttc aaataagcaa aaattcctaa aacacaacaa
      121 tgaaagcgat aactaagaaa atccttacat aacgattttt cttattaaaa ttgttttttg
      181 aacttcgctt attttttgtg agcatatctg tttacttact ttttgtaagg tgctatactt
      241 catttcgtaa tcaattaaaa aaatgcaaaa cgcaaatcaa aggagaaatt ttattatgac
      301 aacttttact gacactttga aaaaccgccg ttcaatctat catctaggac gtaatgtttc
      361 actttctaat gaagaattaa caactttgat caaagaagca attaaagaaa gcccaactgc
      421 tttcaatgct caatcaacac gcgcagttat ccttttcgga gatgcacatg aaaaactttg
      481 ggaaatcact gaagaagcac ttcgcccatt gactccagca gaagctttcc caaatacaca
      541 aaacaaatta gctggtttca aaaatggtta tggtacagta ttattcttca aagatactga
      601 cgtggtaaaa ggtctacaag aacaatttga attatatgca gacaacttcc cagattggtc
      661 agaacaatca aacggaatcg caacagctaa tacttgggta gcattagttg ataaaggatt
      721 gggcgcaaac ttgcaacatt acaacccagt tattgacgaa gcagttgcaa aagaatggaa
      781 tatcccttca aactggaaac tacgttcgca attagtattc ggttcaccag aaacaccggc
      841 tgaggaaaaa gaatacatga acgatgccga tcgcttccgc gtatttggat aattttattt
      901 gaaataaaaa acagaggttg tgacaaacat cttgtcgcaa cctctgtttt tattctggat
      961 caatcataat atcaatctct gcctcatcgt ttaagcgttg ggacaaaaat ggaactaatt
     1021 cttctaatga tgcagaaaat ttcagcatcg gtcctcgcca tccgtttcgg aaaaaattca
     1081 tttttacgat tttagaacct tttggctgat ataatacaat tttattgatc tcagaaaaag
     1141 gaacaccttt tttgtcaaag ctattcaaaa caagtccttc ttctgtgatt cctctactgt
     1201 ccaacaaaaa actaataaaa atcattgctg acaagatccc ttttgccaat tcatctgctg
     1261 taggatgact gatgagtgta taccaaatga ctagtgacac taagaagggt atcaccaagt
     1321 agacccatcg atttcgcaca gtccgataaa taatttcttt tcgaaccagc caaagaaata
     1381 aaaacatcac aacaacgaat aaaaacaata gaaccgtaaa gaatgttacc atgacaactc
     1441 gcctcgcttt ttcttctcca gaaaatagga aaagaatact gatttacctt ctcaaagaaa
     1501 ttggacttgc agcacgccgc acgtccaatt gttttcttta ttcaaatgta tcatattttg
     1561 caacaaccat gcttgctttt tcatctaata attcttggtc ctctgcagca cattgggcaa
     1621 ctttgaccac ggcgcagttt gtcatttttg atacaacttc accaatgacc gtttttgtaa
     1681 aaccaattgg tttgcatgca tattgttctc ctaaattaat catttcttcc gtcatcaaaa
     1741 tcctcctaaa ctgctggaac ttttctccac attaccactg ctatttcttt ggagatttaa
     1801 ctcattgatt tttatttaca gttaaaaatc aaccttacag gcattatagc gtactgtttt
     1861 cgttttcaca attaaaacag ctgttgtatt tagaagaatt tttttcaaaa aattaaaaca
     1921 tattccttaa aaactaatta tttcctacat attaaaatat aatacttcca tttccaataa
     1981 aaatacattt ataataggaa agataaacag ctgaaaaggg agatttcaaa tgaagaaaaa
     2041 aacgttcaaa aaactattgt ttctaggggg cacagctgct cttttattaa gcggtacaag
     2101 ttattaccta catatccatt ctccagaaca tacaatgaac actgcacaaa aaagttcaac
     2161 acaaaaaacg aaaaagacaa aagagaaagc agaagtttta ctagatgttc cattagagag
     2221 ccagtttgat tctccttctt tagaaaatgg ctgtgaagtc acttcgcttt ctatgctttt
     2281 atctttttat ggttatgaaa caactaaaaa ccagttagct gagcaattgg attacgtccc
     2341 tgttttcaat gcagatggga ctcatggcga tcctaatgaa ggatttgttg gtgatatcag
     2401 tggcggagac tgggctatgg tcgtctatgt tcccccagtt gcctcccttg ctcagcaaat
     2461 cgtccaaacg gattatcaca cttttccaaa aaccgatgct caactcgatg atatcaaaaa
     2521 agcactatct aaaggaaatc ctgtctggac ttctgtgact atcaattttg aagtcccaga
     2581 tgaaactgat tttatgactt ggaccacaaa taatggagaa gtaagagtca ctcctcttgt
     2641 tcatgcttgt gtagtcactg gatatgacaa agaaaatatt tatgtcaacg atccctatgg
     2701 tgtaaaaaat cgagcagtac cgatcaacga tttttcggcg attttcactg caatgggtgg
     2761 acagatgcta actttggaga aaagctagta attcaaacaa aaatccctta tactcaagat
     2821 accaaacctt tgaagggagc gtagataatg gaaaaattca cacataaaaa aatggatcca
     2881 aatgaaattc cgattatttt tgtccgtgac tgcaagggaa atgttcaagg taaagtctct
     2941 attaatgagt ggaacgagcg tcgcagacct gctaccctca atgaactgga aatcaaattg
     3001 tatcgtcaat ctcttgttta ttacgcagat caagagtacg agaaagccac cgatttattg
     3061 aaatttctaa ttgctcgaac agagtatact cgctttgaat atattgaacg tttagcaaac
     3121 atctatcata ttatgaatga acctgtcaaa gaatatcagt tattggacac cgtcttgtca
     3181 gtagcagaac tgattgccct tcctgctgga ttagaaaaga aactagtacg ccgcctttta
     3241 cgagtaaagc aacaactatc cgatcaagaa aaataacttg cgttttttta ggaaagtgct
     3301 atgattattt tgttgaataa atgggatgta gccaaactgg taaggcactt gactctgaat
     3361 caagcaattg taggttcgaa ccctgccatc ccaatttgat gtgcttgctg aaaaaatgaa
     3421 ccccctgtaa aagaacaatc tttttgcagg gggattattg tattattttt actacttgta
     3481 tttagtttac ataattaaat tatgaataat aaaatcgggc gtattcttaa tacaaaaact
     3541 gatttaccca ataattgcta atgctttctc cggaactttt tctctaggaa cttcctcata
     3601 cgtttctatg tgtgctcctt tattgctaat tttcaaataa tgattttctt ttaacacaga
     3661 aagaccatta aattcaaccg ttcgttcttt cccattggca tcagcagctt tctgcacata
     3721 ccttgctgta ccatattcat ttacttcctc tggtttttgt gtctttacat agacctctcc
     3781 tttttcaact aatggattta gctgatcaag tataccaggg atttctccat aacttccttc
     3841 agtataatat ttcagtccaa atagcaaaat accactaatc gccgccagtc caatcaaata
     3901 tttaatcact ttcatctttc tacctccttg ttatatattc agtatatggg tcagttaaag
     3961 aaattccatc cgtcccaagt tggagatttt tctcctactt tagaagcgag aatcgagtgt
     4021 ttttttagta aaaatgatcg ttatttgata ttattgacgt gtggataaat cagtttttct
     4081 cgttttgtcc atgtaaaaaa tcatgttgat agcaaaggat gtgttcaaaa tgaatcaaga
     4141 agaattattt caaaaagtca aggaaatgat taagaacggc aatttcgatg gcgcaaaacg
     4201 atttatcgaa gaacataaag aacaattagg accttataaa gagaaagccc aaaatctttt
     4261 aaaagatgta aatatcgaca gtgtcaaaaa caaatttaaa aatctattca aataagtaaa
     4321 actttcactt ttgtaaaatc aaaggaggac aatgctgctg catcgtcctc ctttggttct
     4381 tttctgaata cttcatacta aaataaacgt gtcggatcgt aatgataagt cacattgccg
     4441 ttcagtgagg cttttcccac cacatattta cgattctcca acttcagcca cgcttctcga
     4501 aaagccatta attcttctct ttttacatca atcttttcga tttttccatc gatcaaatct
     4561 tgaataagtt gttcatatgg attcattttt ttcctccatc tgctgtcaat caataagttg
     4621 taaaaatagg ttcatatgct tttttggtat ctagatcttc tctgactttg actgtcgtat
     4681 aaataatttt ttctactggg atactataat aacttccttc accattattg aatcggtgaa
     4741 cgtctttctt tgctaaaata ccatttattt tattcgtcag tacacgttgt tcgacactgt
     4801 tacttacaac atagtaaatc acccgaatat tattgatcaa atgcaaatcg atttctaaca
     4861 ctacatgatc catgaaaatc ccactccttc tttcactttg attatagcaa gtttctcctc
     4921 taatttcaaa atcgatacag attttaccgg ataaatccac aaaactgctt gcgccatttc
     4981 gcacaaagtg ttattttgta tcatggactt tatttatcgg aggcaatagt atgatcgcga
     5041 tcggtcaatt tgttttttat attccttttt ttattatgat aagcattctt ttctattaca
     5101 taaaatggac aaaaaagaag ttttcagttt tactggcctc tttacctgct gtatatttta
     5161 cttatcaaat tttttctttt aggcattggg aaacaacgtc tgtattaata acacacatca
     5221 tcgaattgac gctttcagta atttttctta ttatttggat ctatttttta tataagaatc
     5281 agaattgaat ccctcgcatg tttaaaaaga aaacgattaa agatgcagca aatttctgcc
     5341 gatctttaat cgttttttct attctgttat accaaaaaaa tgtttgaatc ggtgaaggat
     5401 cgttccttgt tttaattcta taaattcttg tgcgatcggg tacttttctt ccaaattttc
     5461 tttgttcttt tcgatcaggc ttttcgcttc ttcatatttt ttatctgcac atagtgcttt
     5521 tacttcctgt aatacttttc ctgcttccat ttgcctcacc ctcttgccat tttattgctt
     5581 taagttttat ttaagttata cgcattattc tagtttcatc ccaaataacc ttttttcatt
     5641 tttactcctc gaactaatca gttttctaaa ggctgattgg ttcgtctttt ttatttttcc
     5701 tccatttcat tctgctcttc tgttaattta tctaatgctt tgattgatgc tgcggctctt
     5761 gcataatcat agctgtataa ttccataaat ttatcaggca gaacaaacca cgctggatga
     5821 taaccgttag ctgtgacagg gaatccttta tcttgttggt tatctccatc ccatctcaga
     5881 gcaacacctt tttttccttt ccactttact aaagcgatcg agtatccctt ctttgtttgt
     5941 gtgccgtctt caataattgc taaaatttct attttttctt ttggcgcacg gacttcttga
     6001 ggacgaacca ttttctattc ctcccttatc tcattacttc acgaccaaca cactgcaagg
     6061 ggcatgtgat acgacataag aagcggtcga tccaactaat gcttgttgta tagcaccttt
     6121 tcctgtcgct cccataacga tcaaatcaat attttcttgt ccagggatga cattggcaat
     6181 caaatttctt ggatctccag tttcaactat tgcgtggatc tcagcaatgc cgtactcttt
     6241 ggcatcattg atttttttca gcatggctac ttctgttctt tgtttttcat ctgcatagat
     6301 ttttgaaaag gcataggcac tatgggtcag ctcaactttg ttgatgattg acacgataaa
     6361 taagtgggct tcattgtcct ttgctaagtc aagggcttca gcgaacgctt tgatcgattg
     6421 tcttgatccg tcaactgcta ccatgatgtt ctggtatctt ttactcattg gaaccgcctc
     6481 ctttttcttt tattgtaaca tctcaagcaa aaatacaaaa aatatacgca atatgtaaat
     6541 aaaatgatga acaaaagcaa tttctttcaa gttatttata catttctttt ctcatcagaa
     6601 cacctgttct aatcccatta tttctgcatt atgtgaaatt cctcatcatt tttcacatcg
     6661 agcgaacatc attctgttac aaaaaacg
//
LOCUS       DAZAID010000121         6571 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000121 DAZAID010000000
VERSION     DAZAID010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.124"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..1022
                     /locus_tag="ACG9BE_002602"
     CDS             15..1022
                     /locus_tag="ACG9BE_002602"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFN3033307.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1034..2038
                     /gene="neuB"
                     /locus_tag="ACG9BE_002603"
     CDS             1034..2038
                     /gene="neuB"
                     /locus_tag="ACG9BE_002603"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFN3033308.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2035..3183
                     /gene="neuC"
                     /locus_tag="ACG9BE_002604"
     CDS             2035..3183
                     /gene="neuC"
                     /locus_tag="ACG9BE_002604"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFN3033309.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3156..3833
                     /locus_tag="ACG9BE_002605"
     CDS             3156..3833
                     /locus_tag="ACG9BE_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFN3033310.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3784..4965
                     /locus_tag="ACG9BE_002606"
     CDS             3784..4965
                     /locus_tag="ACG9BE_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFN3033311.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            4978..5955
                     /locus_tag="ACG9BE_002607"
     CDS             4978..5955
                     /locus_tag="ACG9BE_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3033312.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            5955..>6571
                     /locus_tag="ACG9BE_002608"
     CDS             5955..>6571
                     /locus_tag="ACG9BE_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFN3033313.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLA"
BASE COUNT         2448 a          824 c         1257 g         2042 t
ORIGIN      
        1 aggaggagat ataagtgttt aaaggaaaaa caatattagt aactggcgga actggttcca
       61 ttggaagtga aattgttcgt cagcttttaa aatatgaacc aaaagctatc agaatttata
      121 gtagaggtga agaaaaacaa ttttatatgc aacaagagct ctcaaatcac actaatttga
      181 gatttttaat tggtgatatc agagactctc aaaggattga ttatgcatgt cgtgatgtgg
      241 atattatttt tcatgctgcc gcaatgaaac acgttccagc aagtgaatat aatcctatgg
      301 aagctgtaaa aacaaatgtg atgggaactc aaaatgtaat agatgcagcg atcaaaaata
      361 atgtaaaaca ttttgtaggt atcagtacag ataaagtagt gagtcctact aacaccatgg
      421 gtgcgacaaa gttattgagt gaaaaattaa taatagctgc caatcagtat aaaggaaaaa
      481 cagaaacaat attttcttgt gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta
      541 ttcctctttt tagtaaacaa ataaaaaaca ataaagatat tactttgaca gatgttgata
      601 tgacacgatt tatgatgtca ataccggatg ctgcaagatt agttctgtct gctgctatga
      661 attcaaaatt tggagaaaca tatgtcttaa aaatgccggc aatcaatatt ttaactttag
      721 ctcaatcatt aataaaaata tataatgata cacatgatga aaaatataga ggtgaaataa
      781 aagttgtagg tatcaggcca ggagaaaaat tatatgaaga gctaatgact acagacgaaa
      841 ttgaacgctc atatgaaaat gataaaatgt atatcattcc accacactat catgctactg
      901 acgaactgta caaagattat gaaaaaatta aaatcactgc atactcttca gatgcaacag
      961 aattaatgag tggacaggaa tttattagaa tcgcaaaaga gaataatgtc gtgacttggt
     1021 aaggagtttt atgatgaaat cagaagtata tgtaattgca gaaattggtg taaaccataa
     1081 tggaagtcta gatttagctt tagaaagtat tgataaagct aaagaatgtg gagctaacgc
     1141 ggttaaattt caaacattta aaacgaataa gctagtttcc aaaaaggctc caatggcaaa
     1201 atatcagaag gataatctta aagaggacag ttctcaattt gaaatgctaa aaaaattaga
     1261 attatctcaa tctgatttta ttagaataaa agattattgt atacaaaata aaattgattt
     1321 tttgtctact ccttttgatg aagaaagtgc agactttcta aaacaaattg gtgtacaagg
     1381 atttaaaatt ggctcaggag atttaacaaa tttacctttt cttagaaaaa tagatatgta
     1441 tgggttgcca gttttattat ctacaggtat ggctgatatt catgaggtca gtgaggctgt
     1501 agattgtttc atagatagtc cagttactat tttacattgt acttcaaatt atccggctag
     1561 ttctgaagat attaatttat tggcgattaa cacattaaaa gaaatgttcg ggatacccgt
     1621 tgggtactcc gatcattcat tgggatatga tgttggtatt tgtgctgttg cgatgggtgc
     1681 taaggtaatt gagaaacatt ttaccttgga taaggaacta cctggtccag atcataaagc
     1741 ttctttagat cctcaggaat ttgcagattt tgtaaagcat attagaaaca ctgaaatatt
     1801 tttaggtgat ggtgttaagc gagcaatgcc ttctgaactt agtacaaaag aagtatcaag
     1861 aaaaagccta gtggtcactg agaatttaaa tgtgggagat attcttacag agaaatctct
     1921 tacaataaaa cgtcctggaa atggaatagc tcctaagttt ttatatcaat atattggcaa
     1981 aaaagttaaa agaccaataa gtgcagatag tgttctgctt gaggaagatg ttgaatgaaa
     2041 aaaatactta tagtaagtgg aacacgagct gattatggaa tatataaacc tgttgctgag
     2101 aatttactac aagataattg ggatgtatca tttgttgttt ctggaatgca tttatcccat
     2161 aaatatggat ttactaaaga attgatctta gctgacggat ttagaattag tggagaagtt
     2221 caaagtttgt ttttagagaa caataaggga aacatggctc ggtctgtggc attagagata
     2281 aatggtttca caaatatatt tgaacaaact agccctgatt ttgtattatt actaggggat
     2341 agaggagaaa tgttggcagc tgcgatagct tgtttgtatc ttggaatcaa aacagcacat
     2401 attcacggag gcgaagtgtc tggtactgta gatgaatcaa taagacatgc cataacgaaa
     2461 atagcatctg ttcattttgc tgctacggaa aaaagcaaag agcgtttaat aaaaatgggg
     2521 gaagattctt ggagagtttt tcccgtcgga gctcctagaa tagatactat tttaaataat
     2581 agattaccta gttttagaga tataaaagaa aaatatcatc taaaatttga aaagaaaaaa
     2641 tacgttttgc aagtatttca tccagttgta accgaactag aaaatataga aaaacaaatt
     2701 ttagctttga tcaattcaat ggaacaacaa tctcaaccaa ttatttgcat tttacctaat
     2761 agtgatgctg gtagcgaaat tatcagaaaa atgtaccaga aatatttaaa aacggatatt
     2821 atttatatta cgaatttaga gccggaggaa tacctgacta tattaaaaga gtgcaaattt
     2881 atgatcggaa attcttcttc tgggataatt gaagctgcat cattcaaaat accagtaata
     2941 aatttaggaa ccagacaaaa tggacgagaa caatctaaaa atattataaa tgcaaatcta
     3001 aatgaaaatg aaattgaaac tgctttgaat aaaatacaag atcctcagtt tataaaaagc
     3061 ttgaaaaatg ttaagaacat ctacggtgac ggcaacagtt ctagaagaat aacaaaaaca
     3121 ttgaatcaaa tattgaatga tggggaagat attaagtgga ttcaaaaaca aatagcttat
     3181 taaaatatac tagtgatcca tacattatta ttggggctgg cggacacgca aaagtcatta
     3241 ttgaaacctt aagattagaa aataagttaa tatatggatt ttttgatgat aagcaaaata
     3301 aaatgcaaga tctgaaatat cttgggaaaa taaacgaatt gaatagctgg gcggatagca
     3361 aaaaaaaaat ctataaattt attgtagcta ttggtgactg tgtagtaaga aaaaagatta
     3421 taaaaaaatt gaatatgcca agtgagcaat atggaatagc tgttcatcca agtgctatag
     3481 tggcaaaaga aatttcaatt ggttatggaa ctgttatttt tgcgaatgct gtaattaacc
     3541 cagatgctgt aattggagag catgctataa taaacacagg tagcattgtt gagcatgatt
     3601 gtagaattaa taactatgtt catttatctc caggtgtatg cttagcgggt ggagtacatg
     3661 taggtgtagg aactcaaata ggaattggtt ctcaatgtat ccagatgaag aaaattggat
     3721 cttggtgtat gataggcgca gggagcacta ttgttaagga tattccctca cattccttag
     3781 cttatggcaa tccagctaaa attaagaaag aaggaattaa ttttgaattc tagaatttta
     3841 ttatcaagtc ctcatatggg aggaacagaa gaaaaatatg ttgctaaggc ttttaaagat
     3901 aattggatag cgccattagg tgaaaatgtg gataaatttg aagaaagtat tacaaaatat
     3961 actggttcta agggcggagc ttctgcgtta agttcaggta cagctgccat acatcttgcc
     4021 ttaagactgc ttaatgttgg aaaaaacgat attgtctttg ctccttcatt tacttttata
     4081 gctacagtaa atcctatttt atatcaaggg gctgtaccag tgttcattga ctcagaacct
     4141 aatacatgga atatgagccc tgttgcacta gagagcgcat tagaagaagc tgaaagaaac
     4201 aaccaattac ctaaggcaat catcgtagtg aatttatatg gtcaaatggc tgattatgaa
     4261 cgaattttag ctattgcaaa ccaatttaat gttccagtga tagaagatgc agcggaatcg
     4321 ttaggttcta cttataaggg aatttcgtca ggcttatttg ggacaattgg gatctattca
     4381 tttaacggta ataagatcat cacaacctca ggtggaggag ctctagtgtc tagtgataaa
     4441 aaatatgttg agcattcaag atttttagca acacaagcaa gagatcaagc accgtattat
     4501 caacatagcg aaataggtta taattaccgt ctaagcaata tttctgccgg tatcggacgt
     4561 ggacaaatgg aagtattgaa tgatagagta gctaaacgaa gagaaatttt caatcgatat
     4621 aaggaagctt tttctggaat tgatagaatt gaaatgatgc cagaagtgga tggcatggtg
     4681 ccaaatcgtt ggttgtctac tttaacgatc aatgattata attctattga tcaagtagtt
     4741 aacgtaataa ataaactaaa tgaaaaaaac attgaaacaa gagctttgtg gaaaccacta
     4801 catttgcaac cagtatttca aggggtaaaa atgtattacc atgataataa tcttagtgtg
     4861 tcagaaaagt tatttaatag cggcttatgc ttaccttctg gaagtaatat gacagatagt
     4921 gaacaagaat ttgttattga tgagtttaaa aaaatattca agtaggtgtg atattttatg
     4981 aacataggaa ttattggttt aggttccatg gggaaaagaa gacttagact cattcaagaa
     5041 cactttaatg atattttact atgtggtgtt gatactaatg aggcgagacg aaaggaagtt
     5101 gaaaaggaat ttggaatacc aacttataat agtctaacag aggcaaaaaa tttgtttgaa
     5161 ataaattcag tttttgtttg tacatcccct ataacccatg aatttattat aaaagaggct
     5221 ttaaatttag attgtaatat ctttacggaa atcaatttat taaatgaata ttatgatgaa
     5281 ataatcgaag tagctaaaaa gaaagataga ttactttatc tatcttctac atttttgaag
     5341 agaaaagaaa ttcaatatat taagtctaaa gtgacgcata ataaaaaaat ttcttatcaa
     5401 taccatgtag gacagtattt acccgactgg catccttggg aagattatcg caattttttt
     5461 gtttcaaaca aaaaaactaa tgggtgtaga gaaatattcg ctattgaatt accttggatt
     5521 atagatacat ttgggaaagt taaatcgttt actgtaatga cccagaaatt atccaatctt
     5581 gatattgatt attcggacag tttcactctt attattgagc atgaaactgg tatagttgga
     5641 actttaagtg tagatgttgt ttcaagagta gctaaaagaa atttaactat aattggtgaa
     5701 aagattttaa tagaatggga aggtaacccg gattctctat tttcatggaa tgaagaaact
     5761 tctagtatga cagatattca attgtacgat gaggttatgc atgataataa ttattcacat
     5821 actattattg aagatgcata tttagaggag attaaagaat ttttcacgct tttggaaaag
     5881 aaaatggaac cccaatatac ttttgaaaaa gataagtata ttattaactt aattaacgac
     5941 attgaggtaa attaatgatg aaagtaattt tttttgggtt agggtcaatt ggaaagagac
     6001 atctaaaaaa tctaataagc tatggaaaaa agaattctat taattttgat ataacagctt
     6061 atagtagcag tggaaaaaaa gagtttgaag aaataacgta tattcaaaat agagacgatt
     6121 tggatacaca ttatgatata gcttttatta caaatccgac ttttaagcat ctggaaacac
     6181 taaatgtaat tgttgaaaaa gttgattaca tatttttaga aaaacctgct ttttctgaga
     6241 gtattgatct tactcaatat aattatgatt acaaaaatat ttatgttgct gctccactga
     6301 ggtataaaaa tatcatggaa gttgttcgta atagtattca aagtaaaaaa gtatattctg
     6361 taagagctat ttgttctact tatcttcctg attggagaaa aggtactgat tatagaaata
     6421 attattctgc ctttaaatct atggggggag gagtagaatt agactgtata catgaattgg
     6481 attatattat aaacctattt ggtttcccta aatcagtaaa aaaacagttt ggtaaatatt
     6541 cgaatcttga aattaacagt aatgatttag c
//
LOCUS       DAZAID010000122         6546 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000122 DAZAID010000000
VERSION     DAZAID010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.125"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1273..1938)
                     /locus_tag="ACG9BE_002609"
     CDS             complement(1273..1938)
                     /locus_tag="ACG9BE_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3033314.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="ACG9BE_002610"
     CDS             2064..4100
                     /locus_tag="ACG9BE_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFN3033315.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ACG9BE_002611"
     CDS             4104..4742
                     /locus_tag="ACG9BE_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3033316.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ACG9BE_002612"
     CDS             4770..5198
                     /locus_tag="ACG9BE_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033317.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..>6546
                     /locus_tag="ACG9BE_002613"
     CDS             5655..>6546
                     /locus_tag="ACG9BE_002613"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN3033318.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDS"
BASE COUNT         2242 a          995 c         1211 g         2098 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attcta
//
LOCUS       DAZAID010000123         6490 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000123 DAZAID010000000
VERSION     DAZAID010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.126"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..770
                     /locus_tag="ACG9BE_002614"
     CDS             33..770
                     /locus_tag="ACG9BE_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN3033319.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            784..1599
                     /locus_tag="ACG9BE_002615"
     CDS             784..1599
                     /locus_tag="ACG9BE_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFN3033320.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1612..2259
                     /locus_tag="ACG9BE_002616"
     CDS             1612..2259
                     /locus_tag="ACG9BE_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN3033321.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2275..2931
                     /locus_tag="ACG9BE_002617"
     CDS             2275..2931
                     /locus_tag="ACG9BE_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN3033322.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3093..3914
                     /gene="racE"
                     /locus_tag="ACG9BE_002618"
     CDS             3093..3914
                     /gene="racE"
                     /locus_tag="ACG9BE_002618"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFN3033323.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3917..5272
                     /gene="rph"
                     /locus_tag="ACG9BE_002619"
     CDS             3917..5272
                     /gene="rph"
                     /locus_tag="ACG9BE_002619"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFN3033324.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5273..5791
                     /locus_tag="ACG9BE_002620"
     CDS             5273..5791
                     /locus_tag="ACG9BE_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN3033325.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5898..6398
                     /gene="cbpB"
                     /locus_tag="ACG9BE_002621"
     CDS             5898..6398
                     /gene="cbpB"
                     /locus_tag="ACG9BE_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFN3033326.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2131 a         1092 c         1436 g         1831 t
ORIGIN      
        1 atcttactaa ataataatgg gagtgtaatt ctatggcaat gattaaattt gatcacgtgg
       61 ataaatatta tggcaagttc cacgcattaa aaaatatcaa tctcgaattc gagaaaggag
      121 aagttgtcgt tgtcattggt ccttctggtt caggaaagag cacgatgctt cgctgtatca
      181 atggattaga gacgatctct tcaggtaagc tgctgattaa tgatactgac ttgcatgata
      241 aaaagacaaa actgacagaa gtccgaaaaa acatcggcat ggttttccaa catttcaatt
      301 tgtacccaaa taaaactgtt ctggaaaaca tcaaattagc accaatcaaa gtgttgaagc
      361 aagaccaggc aactgcagtc aaaaacgcgg agaaattttt gaaaactgtc aatatgttag
      421 ataagaaaga ttcttatcct tccatgttat ccgggggaca acagcaacgg gtggcaatcg
      481 ccagaggttt agcgatgaat cctgagatgc tgctgtttga tgaacctact tctgctttgg
      541 atccggaaat gattggtgat gtattagacg ttatgaaaaa attggctcat gacggtatgt
      601 caatgattgt cgtgacacat gaaatgggct ttgccaaaga agtggcggat cgtgttatct
      661 tcatggctga tggagaagta ttggaagaca gcagaaatgt gagagacttt ttcgaaaatc
      721 caaatgaagt ccgagcacag caatttatca gtaaagttat caatcattga taggaggaaa
      781 aagatgcgca ggttaaaaaa aatacgcctg attttccttt taggcattgc ttttcttgca
      841 ctagccggat gtaaaggaaa tgggttggca aatgaagata ttttagagcg aagcaaatct
      901 accaatgaaa tcatctgggg tgtcaagtat gatactcgat tatttggaat gatggatatt
      961 gagagcagaa ctgtacaagg atttgatgta gatattgcaa aagcaatcac taaaaagatc
     1021 cttggtgaca atggaaaaac agaatttgtt gaagtcactt ctaaaactcg gattccatta
     1081 ttgaaaaacg gaaatatcga tgcaattatt gcaacgatga cgatcacaga tgaacgaaaa
     1141 aaacaggttg acttttcaga tgtctatttc gatgctggcc aagctttgtt ggtaaagaaa
     1201 ggaagccaaa tcaaaagtgt agatgatttg aatgcttcta caacggttct agcagtcaaa
     1261 ggttctacat cggctgcgaa tatccgacag catgcaccag atgcaaaaat tttagaatta
     1321 gaaaactatg cagaagcatt tactgcttta caatctggtc agggagacgc gatgactacg
     1381 gataatgcga ttttgttagg gattgcagat gaaaatccag agtatgagtt agttggtgga
     1441 acgtttacta atgagcctta cgggattgcg atcaacaaag ggcaagaaaa cttcttgaaa
     1501 gctgttaacc aagcgctaga ggaaatgcat gcagatggca catacgataa aatctatcaa
     1561 aaatggtttc caaatgaaac tgaaggaaaa gtagagtaag ggggagaggc aatgagcgaa
     1621 ttgatacaaa cttatggacc aatctttgtc gacgggttca agatcacatt gttttcgagt
     1681 gtcttagcac ttgtgtttag tttgattatc ggaacgttaa tggcaatctt ccaattatca
     1741 aagaataaag tagtgagcgc cttagcaaag gcatatgtag aattttttag aaacattcca
     1801 ttattaatca tcgtgatgtt tttttatgtt gtcgtaccga tttattggtt taatctagat
     1861 ggattccaag caggtacgat tggattaacg atctatacat ctgcttttat tgctgaaaca
     1921 gtacgttcag ggattcaaac cgtaccaaaa ggtcaaacag aagcaggact atcttctggt
     1981 tttacctatt cagaaatcat gcgttatatt gttttaccgc aagcattcaa aatcgttatc
     2041 ccgccattag gcaaccaatt tatcaacctc gtaaaaaact catctatttt agcaatcgta
     2101 gctggtttag atttgatgta tcaaggagac ttgatcgcta gtgaaacatt caataccttc
     2161 gatacgtata tcatcgttgg gttattttac ttagttatca cgctaccgtt atcttactta
     2221 atggtttatc ttgagaaaaa atgggcggtt cgttcataga aggaggggaa atacatggat
     2281 tttagcggcg ctttttcatg gatgaatatc cgttttttat tggaaggatt aaaggttact
     2341 gttgaagttt cattgttttc gatcattttt agtttcttga tcggtggtct gacaggtgtg
     2401 attcgttttg cgaatatccc tttcttttcc aaaatccttg gtgtagtgat tgatattata
     2461 cggaatcttc cattattatt gatcattttc ttcacttact ttgccttacc tcaaatcggc
     2521 attcagatga acatcttctg gtccgctgtc gcagcattaa cgatttttga gtcagctatg
     2581 ttgtcggaaa ttttccgagc tggactcaat gctgttccaa aaggacagat ggaagcagga
     2641 ttatcaacgg gactgactta tgtagaatcg atgcgaacga tcgtgttacc gcaagcattt
     2701 aaatcaatga tcccggcaat tgtgagccaa ttgatttcac tgatcaaaga tacttcatta
     2761 gcagtaatca tttctttacc tgaactttct catcaggcac gtattattta tggacagaac
     2821 accaattatg tgcttccgat gttcatcatc atgacactta tgtacttcgt ggtatgttac
     2881 gcgctttctc tgctttccgg ttacttggaa aagagacggt acagttatta atagatgatt
     2941 cttttagcgg ttgtggcaaa ctaaagtcac aaccgctttt cctctttcca ttcgaaaaaa
     3001 agagatttcg attgttttaa gcaagtaaga gcacttgaaa attcacgcta aaaagtacac
     3061 aatagggagg atagaattta ttgaaaaaaa ggatgatacg attgacagat aatcgcccta
     3121 tcggatttat tgattcaggt gtcggcggct tgactgtagt aaaagaagcc ctgaaacaat
     3181 taccgaatga aaatatttta tttgtaggag acacagcacg ctgcccatat ggccctagac
     3241 ccgcggaaca ggtaatccag tatacttggg aaatgacgga ttatctggtg gagcaaggaa
     3301 tcaagatgct ggtgatcgcc tgcaataccg caactgcggt ggctttagaa gaaatcaaag
     3361 ctgctctttc tattccagtc atcggtgtga tccttcccgg tactagagcg gcagtaaaaa
     3421 aaacacaaaa taaacaagtt ggcattatcg gtacgattgg tacggtaaaa agtcaagctt
     3481 atgaaaaagc actgaaagag aaagtaccag aattgactgt gacaagtctt gcttgtccca
     3541 aatttgtttc agttgtcgaa agtaatgaat accattcatc ggtggcgaaa aaaattgtgg
     3601 cagaaacatt agctccttta accactaaaa aaatcgatac attgattttg ggatgcaccc
     3661 attatccatt attacgcccc atcattcaaa atgtaatggg agaaaatgtt caactgatcg
     3721 attctggagc agaaacagta ggtgaagtat ctatgctgtt agattatttc aatttgagca
     3781 attcaccgca aaatggtcgg acattatgcc agttttatac aactggctct gccaaacttt
     3841 tcgaggaaat agctgaagac tggcttggaa tcggacactt aaatgtagaa catatcgaat
     3901 tgggaggaaa ataaggatgc gtcatgacgg gagaaaagcc caagagcttc gcaaactgga
     3961 gcttcaaaca aatgttttca aatatccaga aggatcggta gtgatccgtt ttggtgatac
     4021 aacagttatt tgttctgcaa caatagaaaa ttctgttccc ccttttttga gagaaacagg
     4081 aacaggatgg gtgacagcag aatatagtat gctgcctcga gcaacgagta cgagaaatcg
     4141 tagagaaagc agcaaaggga aactttctgg tagaacaatg gaaatccagc gtcttattgg
     4201 tcgttccttg cgtgcagtag tagacttgga aaaattagga gaacgtagta tcatcgtaga
     4261 ctgcgatgtg atccaagcgg atggcggtac tagaacagca agtatcactg gggcatttgt
     4321 ggcattgaaa ttagccattg aaaaactttt aagagaaaaa gaattgtcag aagatccaat
     4381 aaaagaacat cttgctgcag ttagcgtagg aatcttacca gatgggactt gcgtcacaga
     4441 tttggattac caagaagatt ccgcagcact tgtagacatg aatcttgtaa tgacagaatc
     4501 tggaaaattc gtggagattc aaggaactgg agaagaagca acgtttgatg gtgaacagtt
     4561 gaatgaaatg ctcttttttg gaaaaaacgc gattgaagat ttgatcaaag agcaaaaaca
     4621 tgctttgcta acagagtttg ctcaaaatga tgagcggata gaagaaacaa aaacaatcat
     4681 cattgcgaca agaaatccag gaaaagcaga agagtttcga aatatgttca aagaagcggg
     4741 atatcatgta aaaacattac ttgactatcc tgaattgcca gatgtggaag aaactggcag
     4801 cacatttgaa gaaaatgccc gattaaaagc agaaacgatt gcacaattgc ttgatcagcc
     4861 agtcttagca gatgattcag gtttgaaagt ggatgcttta ggcggtatgc caggaatcta
     4921 ttctgcacgt tttgcagggg aacaaaaaag tgatgcgggg aataacgcca aattattata
     4981 tgaattgaca gatgtgccgg atgaaaaaag aacagcacaa ttccattgta cgttagtatt
     5041 cgcggcaccc aaaaaagaca gccttgtagt agaagcggaa tggcccggtc gagtagcccg
     5101 tataccatct ggcgaaaatg gctttgggta tgatccttta tttatcccag aaggtaagaa
     5161 acaaacagca gcagaattat cgagcgaaga aaaaaacaag atcagtcatc gggctcaagc
     5221 aatgaaaaaa ttgagtgccg agtggaaaca atggctggaa ggagagagat agatgcggta
     5281 tttagtagta agtgataatc atggagacag agatatcctc gttgacttag tagatcgtta
     5341 tcgttctgaa gtggatatct ttttccattg tggtgattcg gaattagaag cttctgaccc
     5401 attatgggat acattccaag tggttcaagg taattgtgat tatggaccag gattcgaaca
     5461 gaaaaaagtg atccaaactg gtcaggatac agtattcatg actcacggtc atttatcgaa
     5521 tgtccgattt ggtttgacac agttagcaat cgaagctcga gctgcaactg ccgatatggt
     5581 gttttttggt catacccatc aaataggctg tgagatggat cagaaaatcc tgtttttgaa
     5641 tcctggtagc atttctcagc ctagaggccc gatccaaatc ccatcatatg ctcttatcga
     5701 aagtttagat gaccaaattt ctgttcaata ctataaccgt gcccatcagg cagttgaaga
     5761 gttggccttt tctttttcga gagagcgctg aataaaaaaa ctctaatttt taataaaaat
     5821 aagctttatt ttatgaattt tgcatccggt ttcggtaaaa tagtagtgtt gaatgaaata
     5881 gaaatggagg ccatcagatg attggaccta tagtaagaga aatgctgctg gaaaatcagg
     5941 aaacgttttt gattcctgca gaaaatgtgg caaatgttat gtatcatcat ccattgtccc
     6001 atggtttact tgtgttatcg aaggtcggtt attccaaaat tcctgtatta ggaaaagatg
     6061 atcactttgt aggactagta agtttatcag atgtggtcaa taaaatgatt gacttgcaaa
     6121 caatcagcat ggacccgtta gaaggactaa cggttgctga tgtgacagaa acagatgttc
     6181 cgacaattga tgaaaattgg gaactagaag aagtactcca tttgttagtc gattcgcctt
     6241 ttttaccggt tgtcacagaa gatcggattt tcaaaggaat cattacaaga aaagagctgc
     6301 ttaaagccgt aaacttcatg gtgcatgaat tagaacgtcg taatatattg tctccgaaaa
     6361 tcgaagaatt aaaagaacaa atcgatatcg tgagctaaag aacgagaaac taaaagataa
     6421 tcaaaaaata agtagaaaaa acagcgtacc agttttgact ttatcttgaa aaagaggctg
     6481 ggacaaaagc
//
LOCUS       DAZAID010000124         6387 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000124 DAZAID010000000
VERSION     DAZAID010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.127"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1175
                     /locus_tag="ACG9BE_002622"
     CDS             330..1175
                     /locus_tag="ACG9BE_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3033327.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ACG9BE_002623"
     CDS             1456..2676
                     /locus_tag="ACG9BE_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFN3033328.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ACG9BE_002624"
     CDS             2673..5393
                     /locus_tag="ACG9BE_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3033329.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="ACG9BE_002625"
     CDS             5377..6321
                     /locus_tag="ACG9BE_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN3033330.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2379 a          987 c         1231 g         1790 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttc
//
LOCUS       DAZAID010000125         6346 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000125 DAZAID010000000
VERSION     DAZAID010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.128"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..1261)
                     /locus_tag="ACG9BE_002626"
     CDS             complement(29..1261)
                     /locus_tag="ACG9BE_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFN3033331.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="ACG9BE_002627"
     CDS             1516..2085
                     /locus_tag="ACG9BE_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3033332.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="ACG9BE_002628"
     CDS             complement(2263..2703)
                     /locus_tag="ACG9BE_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFN3033333.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="ACG9BE_002629"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="ACG9BE_002629"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFN3033334.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="ACG9BE_002630"
     CDS             3657..4580
                     /locus_tag="ACG9BE_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFN3033335.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="ACG9BE_002631"
     CDS             complement(4805..5044)
                     /locus_tag="ACG9BE_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3033336.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="ACG9BE_002632"
     CDS             complement(5049..5468)
                     /locus_tag="ACG9BE_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN3033337.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..>6346)
                     /gene="mef(H)"
                     /locus_tag="ACG9BE_002633"
                     /gene_synonym="mefH"
     CDS             complement(5695..>6346)
                     /gene="mef(H)"
                     /locus_tag="ACG9BE_002633"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFN3033338.1"
                     /translation="REVGVIAVAKNDLKDSYTFLKKEKPIFISVIFLVCIFNLVLSAV
                     LIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGILTALVASKLKLKNLYTLLLICS
                     VSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTMFVVQIYTLVQTQTPPQLVGKIM
                     ATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVASIASIMISIYSKRIFQKLEI"
BASE COUNT         2003 a         1195 c         1139 g         2009 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat tacacctact tcacga
//
LOCUS       DAZAID010000126         6327 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000126 DAZAID010000000
VERSION     DAZAID010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.129"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG9BE_002634"
                     /pseudo
     CDS             complement(99..1229)
                     /locus_tag="ACG9BE_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG9BE_002635"
     CDS             complement(1878..3080)
                     /locus_tag="ACG9BE_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3033339.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG9BE_002636"
     CDS             complement(3140..3343)
                     /locus_tag="ACG9BE_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN3033340.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACG9BE_002637"
     CDS             complement(3485..4552)
                     /locus_tag="ACG9BE_002637"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN3033341.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG9BE_002638"
     CDS             complement(4610..4717)
                     /locus_tag="ACG9BE_002638"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3033342.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG9BE_002639"
     CDS             4880..5692
                     /locus_tag="ACG9BE_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3033343.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG9BE_002640"
     CDS             complement(6066..6257)
                     /locus_tag="ACG9BE_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033344.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1884 a         1298 c          997 g         2148 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaa
//
LOCUS       DAZAID010000127         6181 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000127 DAZAID010000000
VERSION     DAZAID010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.130"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACG9BE_002641"
     CDS             complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACG9BE_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFN3033345.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="ACG9BE_002642"
     CDS             500..862
                     /locus_tag="ACG9BE_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN3033346.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="ACG9BE_002643"
     CDS             914..2596
                     /locus_tag="ACG9BE_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFN3033347.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="ACG9BE_002644"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="ACG9BE_002644"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFN3033348.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="ACG9BE_002645"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="ACG9BE_002645"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFN3033349.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="ACG9BE_002646"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="ACG9BE_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFN3033350.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2055 a         1078 c         1419 g         1629 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaaggatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 g
//
LOCUS       DAZAID010000128         6091 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000128 DAZAID010000000
VERSION     DAZAID010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.131"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..541)
                     /locus_tag="ACG9BE_002647"
     CDS             complement(254..541)
                     /locus_tag="ACG9BE_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN3033351.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="ACG9BE_002648"
     CDS             complement(561..785)
                     /locus_tag="ACG9BE_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFN3033352.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="ACG9BE_002649"
     CDS             complement(1240..1410)
                     /locus_tag="ACG9BE_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033353.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="ACG9BE_002650"
     CDS             complement(1425..1958)
                     /locus_tag="ACG9BE_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN3033354.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="ACG9BE_002651"
     CDS             complement(1979..2923)
                     /locus_tag="ACG9BE_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFN3033355.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3572..3658
                     /locus_tag="ACG9BE_002652"
     CDS             3572..3658
                     /locus_tag="ACG9BE_002652"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN3033356.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3779..4468)
                     /locus_tag="ACG9BE_002653"
     CDS             complement(3779..4468)
                     /locus_tag="ACG9BE_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033357.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4713..5627)
                     /locus_tag="ACG9BE_002654"
     CDS             complement(4713..5627)
                     /locus_tag="ACG9BE_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN3033358.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5609..5989)
                     /locus_tag="ACG9BE_002655"
     CDS             complement(5609..5989)
                     /locus_tag="ACG9BE_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFN3033359.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1612 a         1124 c         1029 g         2326 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gagagcggtt ctgaatgctt atctaagccg ttttaagaca gtcttggata aaaatcctta
     3421 tctagcttta aagcgcctta caggcggtca gggcaagccc taacaaccct aaaataagcg
     3481 atacttaaaa acaccacaca agtttaattt tttaagatat aataacttgt cgcctatccg
     3541 acagtcttgc gactggaagg aggtgtatct aatgtttgaa ttgttcattt gcccccttct
     3601 tatagaagtt tgtgtctctt tattcagtta ttggttgaat aaccgaaaca agaagtaaaa
     3661 tcaataaagg cgacatcaac ctgaaaaaaa gccccagaga atccgacctc tctggggctt
     3721 tcgtgtatct aatgtttgat catttgcatg tttagtttaa catgattgcc gatcaaattc
     3781 aattttgctg ttgctgatct aaccattcct tgacactttt tagataattt aatgccgttt
     3841 cttttttcgt tggattttgt atgttcgttg cttgctgaag cagtgcttta taacgttcgt
     3901 aggtaactga ttgttgccgt tcttttcgtt cctgttcact agttgaaaaa gggattgtat
     3961 cactgaataa attataacaa gcttcttctg tctgcgtatc tttaaaatat tgcacgtctt
     4021 ttttggttcg ctcatagttg tcttgaacct ctttttgctg tttttctggc aagttttgat
     4081 aaagacgagt gagtttttcc atgtttttat ccgtcaaacg tttttcttga ttcacaaaaa
     4141 tatcgttcat ttcctcataa caagtggtaa actcttccat tttttgtgtt aacacttgct
     4201 cctttttctt tttatcacat gctgcttcat acttttgagt gatatcccct aataaaatac
     4261 ttcttgaaat acgtttattt ccctctaaaa cgccgaataa accacttaaa acgattgata
     4321 atgtaaatat aacccctggc aataaaaacc tcttaaatcg aattttagag ccttttaatg
     4381 aatatcttgt ttgataagcc ttatagctaa gccaaataat tcctaaaata ctggcaaaaa
     4441 tgagtagata gggtaaatag aatgccattt ctatttattc tccccatacc agccaaaagg
     4501 ctaacagtgt catgattaag caacataagg gggcaagaat atccattgat cgctgcataa
     4561 ttactttttt ctgttcttta tttcgctggt ttaatagcgc atggtggaat tttttgactt
     4621 gtgaatcaat tgtagtttgt attctttctt tggtataagg tgcaagaaaa gcacgttgtt
     4681 tctttatttc attttctatg tcttgtaacg ttttagaacg ataattcttg ttggtagtct
     4741 gctcctcttt caacgtgttg gtcatggttt cttcgagtcg gtctaatttc cggttgttca
     4801 gtcgtaagag ttcgttgttc tcttcgtact tcttttgcag tttcgcgaat gctagctcga
     4861 gctcttcgta gctcgttggt tcgttgttga ttgtaggctc gttctcgtct gtccagctca
     4921 tgtaaaatcg tctccttttc gtaatccttt cctagtttgt tggatcgtac ttttttcttt
     4981 ttgtggatca attcgtaagt aaaatttttc ccacgattca agcaatgaat gccgaatgtt
     5041 ttaagagaat cacaaaattg gttatacgtt cgagtttttt tgtttttcat tgtttgatca
     5101 atcgcaaacc gaatttcgtc tttccaaaca ggttcatttt tttcttttaa tttgatttca
     5161 gacatggttc gtttttcggg tttcttccct cgttcagtga ttgttaagtc atgttcaaga
     5221 caaatctcgt catttaggtc ataacatttg tttaaaaatg cttgaccatg ggcttgaaat
     5281 ttgttcccag tttccagatt gacggagtta atgacaatgt ggttatgaat atggtctttg
     5341 tctgtatgtg tgtaaatcgc gatttggtgg tcaggggcta tttttttcgc atactctgcc
     5401 cctagttgat tggctttttc agcggtaatt tctcctggtc gaaaagactg gatcaataag
     5461 tgtgcttgta cacgatcgtc ttttccatac agcatgcgaa tttgtttcat ttcgcttttg
     5521 gcataatcaa tgtcacagtt tatccctgtt ttgacggttg ctcgtggttc ggcgtaattg
     5581 atcgctcggc tacaagaagt ggttcggctt actttaactg ttgccatatc ttttgtagct
     5641 ccttttgcat gatttttagg ttttcggata agcgttgggc gtcgccagga tcaacgtcgt
     5701 gttggtgtgt gttgcaccac ttggcaattt gattgatatt cccgccagct ttggctaaat
     5761 cgcgcgcgat tgcctgagca atgtctggcg ccactttcgg tgtcaccaga cgcgcgtttt
     5821 gtgccttggc tttcacgaaa gccggcacac tcatttgcaa agcttcagct gactgcttca
     5881 gctttgcaaa ttcggattcg ctcactcgaa aactgatttg ttttggctcg gatcgatagg
     5941 tatatttttt agaatgattg ctagccaaat attgattttg ttcgctcata agtcccaccc
     6001 tttcagtacg tcctacggac gtatcgttcg caataccatt gtacctaaaa aggtacaata
     6061 agtaaagctc actaaagctc actaaagggc t
//
LOCUS       DAZAID010000129         6001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000129 DAZAID010000000
VERSION     DAZAID010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.132"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            237..491
                     /gene="rpsT"
                     /locus_tag="ACG9BE_002656"
     CDS             237..491
                     /gene="rpsT"
                     /locus_tag="ACG9BE_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFN3033360.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(580..1626)
                     /gene="holA"
                     /locus_tag="ACG9BE_002657"
     CDS             complement(580..1626)
                     /gene="holA"
                     /locus_tag="ACG9BE_002657"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFN3033361.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(1664..3937)
                     /locus_tag="ACG9BE_002658"
     CDS             complement(1664..3937)
                     /locus_tag="ACG9BE_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFN3033362.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(3930..4469)
                     /locus_tag="ACG9BE_002659"
     CDS             complement(3930..4469)
                     /locus_tag="ACG9BE_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFN3033363.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(4605..4823)
                     /locus_tag="ACG9BE_002660"
     CDS             complement(4605..4823)
                     /locus_tag="ACG9BE_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFN3033364.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(4820..5065)
                     /locus_tag="ACG9BE_002661"
     CDS             complement(4820..5065)
                     /locus_tag="ACG9BE_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN3033365.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(5181..5909)
                     /locus_tag="ACG9BE_002662"
     CDS             complement(5181..5909)
                     /locus_tag="ACG9BE_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFN3033366.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         1854 a         1227 c          977 g         1943 t
ORIGIN      
        1 aatagataaa taaaaatcta gtttggtttg acgctattgc ctcccacaat agcgtctttt
       61 tgattttttt cagcttgtac tatccgtttt tactgaaaaa aaatcctaat aaaagactgt
      121 taaggaaaaa aacttgtcat gttcatattg caaagcacgc tgcctttagg tataataatg
      181 aatgttgaat tatgcggtaa cacgcgtaag aacttaagtt atcggaggtg aaactaatgc
      241 caaacattga atctgcaatc aaacgtgttc gtacaagcga aaacgctaat gtgaaaaatt
      301 catctcaaac aagcgctatg cgtactgcaa tcaaaaaatt tgaagatgct gtagcttcag
      361 gtgctgacaa cgtagatgcg ttatataaag aagctgttaa agctattgat atggctgaat
      421 caaaaggtct aatccacaaa aacaaagcta accgcgacaa atctcgttta agcaaaaaaa
      481 tcgctaaata aaagaatcgg cttcggccga tttttttatt tgtcaaaaaa aggacaaaaa
      541 caaacgacct tcccgcatgc tttgccctcg tctttcttct tatcgatttc ttaacaactg
      601 ccctgtgcgc aaaacgaata gttgaaaact taattctttt tcgatttgtc ccgttttgat
      661 ttgataatcc atctcaacta attgatcaaa caactgtacc agcaaatgtt catcgaattt
      721 ttttacttcc tgcatggcaa gttttacacg atatggatgg attttcaatg tttcagcaat
      781 atttgcctgc tgataaccaa tctttacaag aaattttgtc tgtaaaaata aacgtaattg
      841 agaaagcaaa atcgcattaa ttttgattgt ttcttcccct tgcataacta aatcttcgta
      901 taaacgcaaa gcttgttccg tcttcccttt taaaatatat tgggtcatgt caaaaatatt
      961 atgttctaac gttttaggaa ctaattcttc tacttctctt accgtgattt gctgttgatt
     1021 ttgtccataa aggaccaatt tctgtaactc tctcatcatt ttggataaat ctaagtcggt
     1081 caacctcaag aataaatcaa ttgcacttcg ttccattttg attgaggaat tctctaaggt
     1141 attcatcaga tattgacgta cttctttttc attcaactgt tggacattga tgataattgc
     1201 tgtttttttc aattgtttcg ttactttttt ccgttcatcc aacttttcat atggcgcaaa
     1261 gaatactaat acagtcgatt ctaatggttc tttcaaataa gccgtcaata aatcagtatt
     1321 ttgttcgatt ccattgttta ctttttcacc tgttaaaaaa taaggattct ctacgaatac
     1381 taaccgttga tctccgaaaa acggcaacgt ctctgcttca gctactacct catctaaact
     1441 tgcattttcc atgtcaaaac ttaaaaaatt taagtcatct tcgccctcta attggatttt
     1501 tttgacaatt tctgtccgaa cttgatcttg gagaaacttt tctgttccta aaatcaagta
     1561 acaagatgca agtttttctt tacgtatttt ttgtaactct gcttgtatac tcaaattact
     1621 caccactttc aaaaattatc ctaccatgag cagatgagaa ttactagtct atcattgttt
     1681 ccactagccc atgaggatga aaaactggat gccagcgata ggtgatcatt ccttgttgat
     1741 ccgttcgcca tgtttttatc tgattttctt ctagtgtctc caatacttcg ctgtgcgggt
     1801 gaccataacg attatttacg ccgcaggata taatcccatg ttttgcttca atatgggaaa
     1861 gaaaatctgt gttacttgac gtacggctac cgtgatgacc taatttgact acatcggctt
     1921 tcagattagg ataaatcaaa agcaattgtt tttccattgc ttgattcaaa tctcctaaaa
     1981 ataaaaagcg cctattgtta tattccatcc atagaccaat cgaatcttca tttcttccct
     2041 ctcccttttc aaaaggagtc aatacataaa gatcataata accgctgatc ctgtccccat
     2101 gtttgatttc aatgatctca gtaccatttt tttgagcttc agataaatgc ctttgtaatg
     2161 gctcatttcg tccagcgccc caaccgatat aaattttttt gatagtaaaa tgctgagata
     2221 acgtgggtaa ttctcccaca tgatccgtat cgtcgtgtgt caagatgagt ttatcgatta
     2281 cacttatccc ctgacctttt agatatggaa cgatatttct ttcagcagga ggacgtatca
     2341 taccttgcgc ccacttttct ctttcaaagt gtaactgacc tccagtatcg atcaaaatcg
     2401 tttcttttcg aaaaggactt tgcaaaaaga tactgtctcc ttgaccgaca tcgataaaag
     2461 cgatcgttga agaaaagcgt aaaaatggca aagtcaaaaa aataaaagaa aaaagaccaa
     2521 cataaaacaa gtgtttcaca ggttgttctt tcaacctatc catacaaatc agtaatccga
     2581 aaatatacat cccagatagc acaaggggca aacgtcctat aatgagagac gaccctgaag
     2641 aaaaatcgat tattcgctca aataagatta atccctcttc tatcagcaaa agtaaagttt
     2701 ccggcagcca gcgattgaag agtgtgagaa ataacacacc aggaagaaaa agaaacttga
     2761 ataccggtgc aatcagtaat gtaaaaagac tacctaaaat aggccactca taaaaatgcc
     2821 atacaatcaa cggcatcgtg atcatagtaa acaggacgtg agaggccgtt tttttcaacc
     2881 aatctccatc aaaagaaatc aggataaaca aaaaactcat gaaaaaacta agctgtccac
     2941 ccgcatgaaa aagaaatcga ggatcaaata gtaaaagaag ccaaaagaca actgcaaata
     3001 agtccatggt ggaataatgc ttattcagca atttgactag caatcgcaat aaaaacagca
     3061 aactagcacg caaaacagaa atagaacttc ctgcaataga aacataacag atagttagta
     3121 gactcaacgg aataatactg acgtgtagtg gccatccgat acgtcgaaac aaataataca
     3181 gccaccctaa ataaaattgg atgtgtagac ccgatagact gaacagatgc agcaaaccac
     3241 tctccctaaa aatttcttct ggttctctga atcctgcatc tttatagccc cataataaag
     3301 aattaatata tacacttgtt ttttgcggaa attcagattg gacaaaggag ataaaagatc
     3361 ctctttttct ctgaagccag tttcgccagt gattttgcat ggtgatctgt tcaatctttt
     3421 gaatttggaa ccgtccagaa attctatggt tcctgaagta agcttcttgg tcaaaattat
     3481 aaagattccg cttttttgct ggagagatga actcgccttc agcagtcaat tctattccct
     3541 ttcctcggaa gttaagccat gttttcaatt ctgcttctgt gtttacttga taagaaagct
     3601 ggagcttttg tttattctct gataaccttc cttccattgt caaaaaatta ccatttgcac
     3661 gaatcgtatc tgtaaataca gtaatttgcg cagatgttgt tttttcctct aaaaatgaat
     3721 actcttgtag cctataagaa atggataccg ttatgctaca caaaatacac aagtacgcga
     3781 taaaacgtcc ttcttttagc gaaaaccata aaaaaattcc ggcaagaagc aaacgataac
     3841 cccaattgtc agtgtaatac aacaaacaga taatcatcga cgtaattgcc ggaaaaatca
     3901 accgatttcg aacatgcctt tctaaaacgc tagacatgtt ttcctgttaa ttcattttga
     3961 agttttgaaa aatgctgtga gtctaaagca acttggtgta cagatacacc taattgttcg
     4021 atcaacgctt gggcataagg atcatttcga tagtcatgga gataattgat ccctttcaca
     4081 ccagcttgca acaaagcctt tgtacaggcc aaacatggaa aatgtgtgac atagatttct
     4141 gaaccttccg tcgggatacc aagttttgca cactgtagga gtgcattcat ctctgcatgg
     4201 atcgttcgca agcagtgtcc atccacgacg taacaccctt catctatgca gtggacatct
     4261 ccactgactg ctccattata gccgccagca atgatccttt tttctcgaac gagtgtcgct
     4321 cctacttcta aacgtgtaca cgtactacgt aaagataaca gtactgcctg agccataaag
     4381 tactggtccc aaggaatgcg ttcattatgt gacttatcag taactttttc tttattcgta
     4441 tcgcatgctt ttgaagattt ttctaacatg ttttatattc ctccgttttt tcaagttgat
     4501 taaaaagtac tcattgctta aacaaaatat tctttaaata taacgaatca tcctgtctga
     4561 caaaagatcc tacccttcac tgccgaaatg aagtccgtca tccattagcc cattataaaa
     4621 attcttcgaa tccatttgtt caagaaattc cgtataaaaa tcgatacttc acaatcatgg
     4681 cgttttgcta cttctgtgag aacagagata tattctttga tcaacttgtt attccgatat
     4741 ttttgttttg tctcatcgac tgctggtggc gtgataaaga aagcatgatc agaactatag
     4801 tcttttttca gctgaactat catccactgc agattttttg gtacgtttct ttagaaagtt
     4861 gtttatgctc tgctagatca ttcgtcccaa tgagaataaa tactttatct gctgtatagc
     4921 taagtacatc tttggcaaag cgttctcgta gagcttgact gttattgccg ctgacagccg
     4981 tattcataat atgaatccca gatattttat tttggagata gacattcagc attggttttt
     5041 ttaatgtttc tttgcgaaca atcaagctat ttcctatcca aaaatactac ctcctcgtcc
     5101 tgccaagctc ttagaattgg attattctta gtatccatta gcgtccattt attgctcgtg
     5161 ccagtcgatt ttgaatcatt ctatttaatt gtaacgaagt tttttagttt ttcaactgtc
     5221 ttttcaccga taccagaaat ctcctgcagg tcttccactt tctggaagga tccgttttct
     5281 tctctaaatc gaatgatttc ttgcgctttg actgatccga tcccaggaat ctgctgaagt
     5341 tctgatggag tggctgtatt gatatttatt ttgggaggct cggaatctgc tgtaggctgt
     5401 tttccttccg aggatgccaa ctgatcaaga tgttctggca gctcttcacc tacagcaggg
     5461 atatatagga cttgctgatc ttctatcttc gcagaaaagt tgattcgatc gctatccgct
     5521 tcttctaata atccacctgc acgctttagt acatcataga cccgttcttc tgaagaaaag
     5581 gaatagatac caggctcctt cacacttcct ttgatatcgg tgtacatgac ctttggacga
     5641 gcatcctctt ctacatcgtt tccttcagct gatggatctt ctccactgga actaaacgaa
     5701 gaaagtattt cttttgcagc agtctcttct gtttctctat aaaatagcaa atttgataat
     5761 aaaaaaccac ttgcgataca aaagataaga actatcatag acatccaaat gatcaatttg
     5821 ggtgaagcta gtattttctt gctgattttt tctatcattg ttcttttttc aaagaatgac
     5881 cagtaaaaat cattcattac tcctttcatt taaaattgaa gaaacagtga gctctggcaa
     5941 aatcgtccct tcaacatcta attacgtcaa ttttttcttt tgtttttatt tttatcgtga
     6001 a
//
LOCUS       DAZAID010000130         5855 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000130 DAZAID010000000
VERSION     DAZAID010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.133"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(486..989)
                     /locus_tag="ACG9BE_002663"
     CDS             complement(486..989)
                     /locus_tag="ACG9BE_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN3033367.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1348..2373
                     /locus_tag="ACG9BE_002664"
     CDS             1348..2373
                     /locus_tag="ACG9BE_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN3033368.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2696..3574
                     /locus_tag="ACG9BE_002665"
                     /pseudo
     CDS             2696..3574
                     /locus_tag="ACG9BE_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            4050..4166
                     /locus_tag="ACG9BE_002666"
     CDS             4050..4166
                     /locus_tag="ACG9BE_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFN3033369.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5094..5432
                     /locus_tag="ACG9BE_002667"
     CDS             5094..5432
                     /locus_tag="ACG9BE_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033370.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5526..5756
                     /locus_tag="ACG9BE_002668"
     CDS             5526..5756
                     /locus_tag="ACG9BE_002668"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFN3033371.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1994 a          880 c         1077 g         1904 t
ORIGIN      
        1 taaaaaataa cgacacagaa aataccgtgt ctcagatttc tctactactt ctaaaaacta
       61 ctgttttttt cctttgaaat gccttcaaaa agtaggatca ggcagaatat actggcagaa
      121 tcaaggagaa gggagcctaa ataaggagaa aaaccataga aaatataaag taattactta
      181 taaaacaagc tttttataga aaaataatat acctgagtag ggtataaatt aagacggaat
      241 tcctttctag attgagatat tgactaggaa gaatgttaag attttaagcc tttaagctaa
      301 aaaaaaaccc tttttgttaa ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg
      361 tcctgctgca ataatatagt agattagaga tgaaaataga tttttttaaa ccaatgactg
      421 aataaaaaga ccgtcataga tacacattgg aatgtgtcta ttacggtctt tttttacgaa
      481 cgattttatt gaagacggac agttcgggtc aaaaataaaa aagctacgat cagaaataag
      541 aataaaatgt atcccaaaat aatggctaga cggtctaact tatttgtcga tagatgtttg
      601 tttagaagtt gataggccca taaacctatc agtcctcctg ttgtattagt tatcaaatca
      661 gtgatgtccg ctacgccaat accgaatagg aattgaatca attctgccaa gaagcttaca
      721 aggaaaataa tccctaattt tttagaaaaa gaaagtctct tgaaattgac attcaaaagc
      781 accccaaaag gaaaaaagac aatcaagtta taagtggtct cacgtaatac cgtcgtattt
      841 tcaaaagaaa aagtagaaaa agggacgaga ttgacattgc tgtaagaata agtgagaaat
      901 tgtggaagat taaatgatag cttgaataaa atcaaccata atagaaagaa taaataaatc
      961 cctaataaaa attttgatac ataccgcata gacatctcct ccatttaaaa agtataccat
     1021 cgaaaaccgc tgaaaaatca taaaaaagta tgaagtttaa ggaccaacgt agttatcgta
     1081 cgaaaattag aagctttttt ttgttgggaa tccggtttct ttaacaaagc taagatagcg
     1141 agttgcttgg aaaagttgct atcttttttc tgtcaaaacg actcggaatg ttcgtttttt
     1201 gttctaattt ctccaagttg tttacttttt gatactaagt ttatgaaaaa tcaagaaata
     1261 gttgttatac taggtaatgc tgacaaacgg gattggcatt tcatcttggt tcagcttttt
     1321 ttccaattac taggaaggag ggatagattg ttcaagactt attttcgttt caataaaata
     1381 caagataatc cttttcgtat catgaatctg atgctactta tctttattgt attatggtta
     1441 ggttacataa atcatgattt tttgatttca agtttttcat cattagggat ctttactttt
     1501 ttctattatc aaaacatacc gatgaaatat ttgctgaaac gtatgtttct gatcggttgc
     1561 ggactgttgg ttgctttcgt tttggggatc ttatcgactt atgttttatg gcttgaacca
     1621 tttgccgtag ctttggttgc tttttccagc agattcgtgt tgcgattatt ccatatttct
     1681 aaaccaggtg gcttgttttt tgcgatgctt tctgccatgg gaacgagcat gcagctgcca
     1741 atcgctcaat taccgatcgt ctcactttat ttttttatgg gtgtcgtttt tgccctgatt
     1801 gcggcagtga ttacaaaatt actggattca cggcctgagc agacaattga aaaagcaact
     1861 ttaaaagaaa gatttcacga agagccttta gtgatcattg attctgtttt ctacagcgct
     1921 gccttatttc tttccgttta tgtcagtcac ggcttgaatc tgcataatcc ttattggttg
     1981 accttgtcat gtgcatcgat tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa
     2041 gtccaatatc tgattggatc gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg
     2101 ccgtttacgc agttgcagac catttttctg atcacttttc tatatggaat cgcacaattt
     2161 ctagttgcta gaaattatgc agttgctaat atttttctta atccgatggc gttgatgcta
     2221 tctacactta ttcgtggagc atacttgatt tctttgatcg aatatcgttt tttagggatc
     2281 gtcataggaa gtttcatcgg tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat
     2341 tatttaacgg ttgttcgtca aaaattagaa tagaaggaca aatatatgtg ccaaaagagg
     2401 ttatgacaag atttttgtca caacctctaa ttcaaggtgt tcaaaagcca gattctcagc
     2461 attaagtcaa attcacacaa aaatgtggaa aggaactttc aaaaaaattt tatatttatt
     2521 ctatcaaaaa attttgttta tgatggtaac tgaataaact ttcctgagat ggtcaatgct
     2581 ttccttgaaa gtcttacaga tattctttct tcatagaaag tttaagtttt atagttagtg
     2641 tacaataaaa gtatgtttat accatatggt gtccggataa tcagtcattt aaacaatgag
     2701 gttcaaagat atgaaaaggg gaaatttgat gaagaaaata agcaatgaaa tccaaattct
     2761 ggcagaaact ttaactcttc ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa
     2821 catccttgat gaggtgtttg agcaatatag tcatgaacta atggatccta ataaagcaaa
     2881 ggcgactgca aagaaaatag acaaagtcta tgaagaggaa ccttttgcag cattagctat
     2941 ttatctatat gcagcttctc aaagttggaa aaaaatttat caaccattga agattccaag
     3001 aactatctat ttggcaacaa tgaatgcttt tactagattt attcatgaac atttccaagt
     3061 aacaggaagc tatcgctttg accgtggttt ttggatttgg cattacacta gtggcttgat
     3121 ctttcggatt ggtgaattgg agtacgaaat gacatatttt ccgaaagggt acacggatct
     3181 aagattagaa ggaaagcagt gcctttctct tcatattcct tccgatgcag atttatctca
     3241 gaataagata agagatagtt acgaaagtgc cattcgtttt tttgaacttt attttcctga
     3301 ttaccattac caagtgatgt acacagatac ttggttacta tcacccaatc ttactaaata
     3361 gttgaaaaaa gagtcaaaaa tctgtctttt tgcagccgat tatcgattat tatcagttga
     3421 tgaacaggac gatagtggag tcccttggat ctttggacga gtagatgccc aaatacatga
     3481 ttatccagaa agtacgagtt tacagcgaca agcaaaagag caattgcttg caggcgagca
     3541 cattggctct ggcttaggta ttcttacttc ataagtttag tacttcgaaa tagtttaaaa
     3601 gagcgtgaga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg
     3661 gaagcagaag caactccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt
     3721 tcgtgaattt cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttagtg
     3781 ttctatcgaa cacacagtac gttcaatagg taaactagct ataacaatag atattaaagt
     3841 taacggacaa gagggccttt tttaggtagc taaaacgtaa taatgaaaat ttatttccag
     3901 agataggagt caatcagcta gtctgatcga ctgttttaac aagttgactt tcattttaac
     3961 ttttgatacc atttaaaaca caaatcgtaa ttgtttcgat ttgtgttttt tagcatgact
     4021 acaatcaaag aagaaagtgg gtcgtccata tggaaaaaac agatatctca agtgcatacc
     4081 gccgcttgaa aagcccaaat atcaaaacac gaaaacgtgc gctgaagatc atcaaagatg
     4141 tgaaacggaa tagtgggaaa aggtaagaag aaggaaactt aattaaaaaa atagccgaaa
     4201 aacgtttttc ggctattttc aataatcggt ttttgtgtaa ggtttccgaa agaactgctg
     4261 ttgattttcc atttttaaca tatgataaat tggtagaatc tttcttttca aattgatacc
     4321 attccctaat tccagttata tagttaacta cagaaccaca aagcacgtac gtttataaaa
     4381 gtattatctg tagttatttt tcaattccct ttcaacctct tattttgacc agtagtattg
     4441 atcactttac cttttaacac ctttgttcgt ttttgaaaca tttttagtca agaatggaaa
     4501 cgtaactaca taatcaaagg ttttgcgaaa tatttcatga aatatttcat gtagaagcga
     4561 gcaatacggt tgcaaataac cattatcgtt taataagcag ttacgaaagc tcccaatatc
     4621 tctaacgttc agctcactcc atttcttttc ttcaaaaaaa tcagtgattc gtatctcttt
     4681 ctatttgtcg aaaaagttaa ctttaaaaag taacgccgaa aaaatatgca aaaaaataaa
     4741 aaagcagtta ctattgctag aataacatca aggaataata agttttggtt ttcagataat
     4801 agtcgataag ttgtgtattg gaattttaga tacagtaata aagtattttt catgaaattt
     4861 tgtgagaatc actattacaa gtatctttaa taaaattaat ggttaaggag gaacttaaag
     4921 tagaatgtaa atgaatagtt gtagtcagtc gattgtgata ctagactcaa agttgcagga
     4981 ataagttttt caatttgtga ttggaagata aaaaatgaaa aagaagggct tttttttata
     5041 ctttgttgat tgtagtgttt attttccctc tgtttagcca agtaacattt tctatgactc
     5101 aaccagtttc aagtgaggaa catgataacc tttttcgtgc tacagaagag tttaagaaat
     5161 atcaggaaaa tattgttccg gagactccat tagctagtga gccaattact gatattgatg
     5221 gaaaccaaat aggatggtat atacagtacg aagttatggt ggtaaataat gactcaatag
     5281 taaaaaatac agtaggctta gagctaacac cgattgaaaa ggtgagttca ttgttaacgt
     5341 atatttatac tgatgaaagt gacgagactg agatcatttt aacggattac tcaaaaattg
     5401 ctactgataa aaagtatata ttgtggatgt agacactagt gaaatacttt atgatataga
     5461 aattgatgaa aatttactag atgtagcaca agaaatcgat catcaaaagc aggaagctat
     5521 tcgatatgct ggtagaaaag ggaaaagaaa atatttatta cgtgaatgta gcaaaagtta
     5581 gagaagatga aaatgagtgg aaagaattca agagtcgcta ctcaattaac tcgactccga
     5641 catttacagt ttacagagaa ggatcgatag aaaaaactgt tttttggact aaagaaagtg
     5701 gaatgtcttt agctgaagta gaagagtttt tggattatgt tagtatgcaa cagtaaggga
     5761 gttcttatta tgaaaaattt tgacacatat tatgttattg ttttttcgat atctttgctg
     5821 ttaatattac taactttaat gcagtataaa gagta
//
LOCUS       DAZAID010000131         5781 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000131 DAZAID010000000
VERSION     DAZAID010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.134"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..539
                     /locus_tag="ACG9BE_002669"
     CDS             51..539
                     /locus_tag="ACG9BE_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN3033372.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            835..1116
                     /locus_tag="ACG9BE_002670"
     CDS             835..1116
                     /locus_tag="ACG9BE_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033373.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            1118..1534
                     /locus_tag="ACG9BE_002671"
     CDS             1118..1534
                     /locus_tag="ACG9BE_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033374.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            2148..3302
                     /locus_tag="ACG9BE_002672"
     CDS             2148..3302
                     /locus_tag="ACG9BE_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFN3033375.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            3275..3718
                     /locus_tag="ACG9BE_002673"
     CDS             3275..3718
                     /locus_tag="ACG9BE_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033376.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            4852..>5781
                     /locus_tag="ACG9BE_002674"
     CDS             4852..>5781
                     /locus_tag="ACG9BE_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN3033377.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKE"
BASE COUNT         2157 a          983 c          943 g         1698 t
ORIGIN      
        1 aaaacttact agataatctc caaaacttat ttaaagaata aggggttttt atgacagata
       61 aaactatcaa agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac
      121 tgaggaaatt acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag
      181 aagcagtaac aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg
      241 aattacccga ttcattacct accgattacc tactcaagga gattgaaact aagaataatc
      301 atattgaaga aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa
      361 aattactcga ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc
      421 acgaacaact ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta
      481 ctgaagaaac tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac
      541 ccaacaattt ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat
      601 agcgacatga tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc
      661 ttacgaaagg tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca
      721 ctttttcgtc attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat
      781 actatatttg tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac
      841 attttagttc ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa
      901 ctcacaacaa aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa
      961 aacgattttg accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa
     1021 aaatttatcg atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac
     1081 aaccaactca ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa
     1141 acacgttacc ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc
     1201 agatatcaat aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac
     1261 taatacgaca actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat
     1321 tatgtccgaa atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat
     1381 cggtacaaat tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc
     1441 ctcccgacga aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag
     1501 aaattttgac ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt
     1561 tctcagtttc ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg
     1621 cacaaaaaaa gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc
     1681 caatctccaa atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg
     1741 aaataaaaaa agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga
     1801 aaaagcagcg cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa
     1861 gtttctaaaa aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa
     1921 tcaagcttcc gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcggctgg
     1981 atcccttggt acaggcgtta cttggtagta tacacattat gttaactaac gctataaata
     2041 acgattatgt tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt
     2101 gtatatttta attataggtg tataccactt aaatttaaca caaaggcgtg attattttga
     2161 aacccaaaaa gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta
     2221 taggaattac aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa
     2281 ttgaagaaca agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc
     2341 ttttaatgga aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa
     2401 tgctcgatgg agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg
     2461 ataatacgat taaatatatt tataccgtca aaaaaaatac aaatggagaa tattcaggaa
     2521 gtatttcaca atttttagca aaagaacttc aaaatctagt agatagtaat ataacagata
     2581 caaccttatt ttttcaagat aatagcattt attattccga taatggacaa attaaactac
     2641 tttggaaatc acctttaagt acagaaaaaa atggtgtttc ttcccttaca gaaaaaaatg
     2701 atcttatatc cgtaaatata aaaaataaaa ttaaaaattt ttctaaaaaa caaaattttc
     2761 ctgcggttaa acgtgtaaaa agtgtctcct ccggagataa ctttattatg attaattggc
     2821 gtatagcaga aacccaaaca agttttccgt ggtgcgctgc ttacgtagaa gctgctatct
     2881 tatgtaacaa aacagatgta cgtgctactt cagctagagc cattatgcat tatacctatc
     2941 ctaatttaaa caacagtcaa attgaatcaa aaagtcttaa tcaagatcaa ataataaaat
     3001 atgcaaatta ctgtggtgtt tatcctaaaa aaatagcacg aatactaaac ctttcagagg
     3061 tccaaacaca actaagaaaa ggaaatgcca tttatatggg aactgctggt tatggaaatt
     3121 atagtaacag ccgtcatgca cttgcattat ttgggtgggt caatcaaggt ggttatcaaa
     3181 cttactatgt gtggaatcct tggtataact acccaactgt aataagtgca aatgcaggac
     3241 cagttacaat tcctgtacct ggaggtggtt ttacttggga caataccatt acaaattggt
     3301 aaataaactc attgctttag ttatgggtct agtgttagtt cttttcttaa ctagttgttc
     3361 aaaaaaacaa gaaatacaaa agactgatct gattcctttt ctaggaactt ggcaaactaa
     3421 agatcattca gaacgtctag taatcactaa tttaaatact tcaaaaaata tagaacaata
     3481 taccatctta gtaaaagaga acaatcataa aattcaaatc attgataaaa atttaaatat
     3541 aaatgaacaa gttttcattt acaaagcaac aaataattta ctaacactgc gctcgaataa
     3601 aaaacaatta gattacactt ttagtttgga ttctccaaaa aagctcgtat ttattttgac
     3661 aacgaatgat gggacagaag ggacagctaa accaattgaa ttttttaaag ctgattgatg
     3721 aatagttgtt tgatattaca aaacaataat ttcaccgaag gtgaaacaag cgaaacttta
     3781 caaaaagtgg ctagcatttg ctagcctttt ttgtattgtg gagcgtgggt tttgaggggg
     3841 attcaagggg gagcttgcgc ccctttgaca agcggttatt tgcaccaaat acgtaggtat
     3901 ttggctagca aatatagagc ttgccaagcc ctttagtgtg ttttgtagaa agggacctta
     3961 tagtcacttt cacattacaa acgatacgtc cataggacgt atcaaaaggg tgggacttat
     4021 gagcaaatta aagcaaaatt tggctagcaa tccttctaaa aaatacacct attgatcaga
     4081 gtcaaaataa atcagttttc gagtgagcga atccaaattt gcaactctga agctttgcaa
     4141 atgagtgtgc cggcttccgt aaaagtctag gtaacattag tcgatatata catagggctc
     4201 taatttgcgt tttaaggcgt tttaaacgtt ctggtatgta attataccaa agcgacttta
     4261 aaacgcctta aaacgaattt tagaggttct gtagaacgtt ttagggttgt tagggcttgc
     4321 cctgaccgcc tgtaaggcgc tttaaaaaca agataaggga ttttatctag acttgtctta
     4381 aaacgccgta gatgagcgct cagagcttct aaaaggactc cttacgaact gtttttaagc
     4441 aaaggagcct ttaggcgact gcgcagttta atgtgagagc gtagcgatca cacctttaaa
     4501 gcgaccgtag ggagcagatt cccagtttag gaaatttcgc gaacctgttt ggagaaaaaa
     4561 agtaggaaaa ttttaggcgg gaaattttgc ccccctcttt tataatctct tttataacct
     4621 ctttttaaac ctctttaccg tcggctgtat ccttactctc ccaaggtgtg cagaatcttt
     4681 taggggtctt tttgtctgtt tttaggggtc tttttgtctg tttttagggg tctttttgtc
     4741 tgtttttagg ggtctttttg tctgttttta ggggtcttta attagggggt tgacccccat
     4801 tagaaagctc tttataattt tgttaagtag ttaatcttga aggaggattt tatggcaaat
     4861 gaaattgtga aataccacca tgaattaaat acgattcctt tacgcaaatt tacgtcagta
     4921 gaaatgaatt tattcttctc tataatatct agaatgcgtg atgtaggcga taaaaaagta
     4981 caatttacat tcgaacaatt aaaagattta agcaattata aagcaactgc aaatgttcga
     5041 ttcatagatg atttagaaac aacgtatgac aaattaatgg atttacgttt tggtagaaga
     5101 agtgctgacg gtttgcaacg tgaacgattt gttttattta atcaatttaa aatcgatgga
     5161 aaagctgata ttccttttgc tgaaatacaa gtccatgaga aagccttacc cttacttaat
     5221 aatcttgaag aatgggttcg atattctctc caacaattca atgaattaga aagcagttat
     5281 tccaaaacca tgtttagatt attaaaacaa tacagaacca aaggaattgc caatttttca
     5341 aaagaagact ttcatgaatt acttgatatc cctaaaagtt atagagaaag tgatataaat
     5401 aaaaaaatta taaagcctat tcgccaagaa ctaaccgcaa tcttcaaagg tttgacaatc
     5461 caaaagagat acggaaaagg acggggaaag cctgtcattg gctatcaatt tacatttaaa
     5521 cctgagataa aaaaatcaga cgactttaac aaacatgctc caaccaaaga agaacaaaat
     5581 aaagctttgg aactagctaa tcaacaggtt caatctgaaa aagaacctac ttcattacaa
     5641 gaaaaaatca aagaacaaaa acgaaaatta gaacttttac aacagttagc agaattagaa
     5701 agaaaacaaa ataccccaag taagaaaatt aacaaacaaa tagcagataa caattccgat
     5761 attaccccag aatttaaaga a
//
LOCUS       DAZAID010000132         5727 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000132 DAZAID010000000
VERSION     DAZAID010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.135"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACG9BE_002675"
     CDS             196..921
                     /locus_tag="ACG9BE_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033378.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACG9BE_002676"
     CDS             911..1420
                     /locus_tag="ACG9BE_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033379.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACG9BE_002677"
     CDS             complement(1490..2938)
                     /locus_tag="ACG9BE_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3033380.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACG9BE_002678"
     CDS             complement(2938..3654)
                     /locus_tag="ACG9BE_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFN3033381.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACG9BE_002679"
     CDS             complement(3635..3985)
                     /locus_tag="ACG9BE_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFN3033382.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACG9BE_002680"
     CDS             4129..4902
                     /locus_tag="ACG9BE_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN3033383.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG9BE_002681"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG9BE_002681"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1123 c          994 g         1948 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tttttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DAZAID010000133         5404 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000133 DAZAID010000000
VERSION     DAZAID010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.136"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            272..621
                     /locus_tag="ACG9BE_002682"
                     /pseudo
     CDS             272..621
                     /locus_tag="ACG9BE_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(893..1183)
                     /locus_tag="ACG9BE_002683"
     CDS             complement(893..1183)
                     /locus_tag="ACG9BE_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033384.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1585..1821
                     /locus_tag="ACG9BE_002684"
     CDS             1585..1821
                     /locus_tag="ACG9BE_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033385.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1828..3780
                     /locus_tag="ACG9BE_002685"
     CDS             1828..3780
                     /locus_tag="ACG9BE_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFN3033386.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3852..4349)
                     /gene="dfrG"
                     /locus_tag="ACG9BE_002686"
     CDS             complement(3852..4349)
                     /gene="dfrG"
                     /locus_tag="ACG9BE_002686"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFN3033387.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4676..5368)
                     /locus_tag="ACG9BE_002687"
     CDS             complement(4676..5368)
                     /locus_tag="ACG9BE_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN3033388.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1743 a          947 c          883 g         1831 t
ORIGIN      
        1 ttataatgtt ctatcgaagc acccaaatag ccaagttcat ctaatctact tgcataaact
       61 acgtcatccg ctctcatagt cggcatatac atattaaaga gtaaaaagat accccaaatc
      121 cctattagta acaagatcag ttctttgtta ttcgttattt ttctattatg tttgttcatt
      181 tttatactcc ttcttatatt tcctaataga gtataacata gctttaagca ttacttaaag
      241 ataacttaaa agttatcttt taaatactta attgttaaat accttatttg atgcaaaaga
      301 aatctacgat agtcaacaaa aaaattttca ttatatccag aaaaattttt cttagttaag
      361 gacctatgga ttgcagtaat gcagaattcg tcgaataaac tgccaaagac atacaatcat
      421 attgctttta aaattgacga acaagatata gattcgtttg tctcaaaaat ccaaatgtta
      481 ggtttaactg tcgaaccagg acgttctcgt gtgaaaggcg aggcttcttc aatttacttt
      541 tacgactatg ataatcacct cttcgagtta cataccggta ctctccagga acgtttaaaa
      601 tcctacaatt ccactacctg agactagtaa tgactagtct ctttttatgg cctctgatcc
      661 ccccattgag cgaatactaa tagggcttcg aattgatctg ttcaatcttt aagcaatatc
      721 cctcaaatga gtttggaaag tttagtgatt catcacaaat acttaaatac atgtagttat
      781 atatagattg aggttcttgt gagcgtatgc tcgtgtgggt tcaaatccca tcgtctgcat
      841 ccatctttcc aaaacaagcg gctaggacat ttttgtctca gccgcttgtt ttctagttta
      901 ttctttcatc ttgtcggacc ttttttcttg ggtatagact tttctcatat tctaccacaa
      961 ttttgggttt ccctaacaca tgataggatc tcacaacaaa ttgctttcta ttattataat
     1021 aacctgtaac gactaacttc atcccttcgt ctacttcagc taggaagttc aaactatggg
     1081 aagcaatcaa acaattgacc ccatttaaac tgaaacggat caacggtctg gcactaactt
     1141 gcaaagtacg gatcttatct actgttccat attttttctc cataaccttc acctcaatac
     1201 cattatacga acatacattc cctttgtaaa gaagaaactc tgttttatct ttttgacttt
     1261 ttgtctatta cctttcttaa aatcatattg gcacttcctg cgaagatgaa ggacatgtac
     1321 taaaaaaaag aggcagcagt atagatttaa gtagttcagt tttggagtac aaaaagtgaa
     1381 aatgtaaacc taaatgagta tcaaattgca actgaatttg atactcgttt tttattgtta
     1441 aatcaacgtt tgttgttaat ttacaccaca aaatgtaagt taaaatgtat ataaaaatgc
     1501 gaacgtgaaa taattattgt aggaatgcaa attggtgtgt taaaatggtt acaatttact
     1561 aagcaaaaag gggtgcattt tcctatgcaa tttcttgagc caaaaaataa gaacgctaag
     1621 tctgtggatt gggaaatatc agagcaagta agggtgatag ttaagcaata cgctgaatat
     1681 gcagagcgta ctgaaagcga agcagtggat gaattcttat tgaacatttt agacgataag
     1741 aagtttattg aatggattgc caacaagcga agtaataagc ggattgttga aaaaatgggt
     1801 atcaaagata gagtgggttg atagacaatg accaagctta aaagattagc cacgaaggaa
     1861 gatgaaattg tagatgttaa aattccgatt tcaaacgaag aattaaaaga tagggctaaa
     1921 caatatgatt tattaactcc aaaacgattt gcaacaaggt ataacaaaat gttgttcttg
     1981 ccaacgtctt ttaaatggaa cggttctgaa tatcccattc aatataatta ctgcatcaat
     2041 ccgttttgtt gtaattttgg gaaagaacag cacaaattca aagatgtaaa agggaaacca
     2101 agtcgatata aaatgactgg tagttccaaa gataaaggtc ataaaggtat gtactgcaat
     2161 gacaatccta ttggcagagg tgtatcgcaa aactgtacag taaccccttt atctaattgg
     2221 tctgttgttg aagaaattaa acgccttata gaaatcaata gcatccaaga tgttgagcca
     2281 gattatcaat ttcataaaga aggctgctcg gaagaagaat caaccccatt caatgagccg
     2341 aagcaatttt acaaaagggg gaaaagtagg ggcaaatctc aacgctatca atgtaaggcg
     2401 tgtaagaagt tcactaatgt tctaccaaaa agagaagaaa ccactactta tcaccaacaa
     2461 aagaacacca ttcttccaat gttcgctaag atggttgttg gaagggtttc agtcagtcgt
     2521 acttgtgata ttttaggaat tggagttgga acctactatc ataagctgga gtggctgtat
     2581 aggcgttgtt tggagttttt agaacgatat gagacacaac cccttcaaac aaagaaattt
     2641 aacgagatgt ggcttaatac ggacaagatg cactactacc taaataacgt ccgtaagaaa
     2701 ggacaaggtt caaagaaata tacggggttt gaagacctta atatgcaaac gtatatagtt
     2761 gtatctgctg aggtcttatc aagatatgtg ttccgttctg atgttgccta tgattggaat
     2821 atttcaatgg atgaattaaa tgaagataca agaaaattca aagaagacca tctcaatact
     2881 tttagtcgta aaaatgatag gttagattgg tcttattatc cacaagaacc atccgctaac
     2941 gatagcgaaa ataggaatgc ttatcttcac gaattaggga aaattactaa taggagtaga
     3001 tttgtcgatg gtttaaatgt tgatgctcct tatactacaa ctgctcacta ttggttaatt
     3061 aaacaaatgg ttaatgccga cgaatggcga atgattagtg atgatgactt ctctattaga
     3121 aacgcctttt atagagtatt cacgaaagaa ttaaggttga gcgatgcaca tcattttatt
     3181 tgccaagtta acaagacgaa aagcagaaaa caatgcttaa aagaattcgg acaagcaaaa
     3241 gcagaactgc tcgattgggg agatatcaga ggattcaaaa caaagtttct acggactttg
     3301 gcatctcatt atctaactga attactaaca agtcatcaat tccacgaaga ggctattagt
     3361 aaagatggag aaagatatcg caaatatgcc gataacccta tcaaacatcc attagccact
     3421 aaggataaag gattttattc agttgattgt agaacggatt tatctgctct ggaaccaaac
     3481 gaaatcgcaa agatgctatt aaatgttaat gaccattcga ccaatagctt tattcaacag
     3541 ataagacgtt atatatcatc tcttgaaaga ccattaacaa cagctcgtgg tgataagaag
     3601 agctatattt acgctaactt caatccgaag tatgctcaat ttgcgattac aattctaaga
     3661 acttactaca acttttgtcg cccttttaaa tcagcggata aaaaggtttt gacacctgct
     3721 caaagattag ggattacaga taaacaattt gattggaaag atataattta tttcaaatga
     3781 caaaaagagc ttggagtaat cccaagctct tgtgctttat ctttactgtt cttcttcaat
     3841 taacgcaccc gttaactcaa taagtttttt ctttcatata catgaaaata gtagttatac
     3901 ggatttttat cattctttat ccctttttgg gcaaatacct cattccattc ctcataattc
     3961 acttctggaa aaaaagtatc tccttcgaat tcatgatgta tttttgtgat gtacattttc
     4021 tcaacataag ggaaaaacaa attataaatc tgttctcctc cgaaaataaa aatttcttct
     4081 tcgtttttac ataactcaaa aacatcttct attgaatgaa caatttcaca accattaaag
     4141 gtaaacccct tatctctcgt cagaataata tttcttctgt caggtaaggc tcttccgatt
     4201 gattcaaggt tcttcctacc taatattatc ggatgtccct ttgtagtatt tttaacatat
     4261 tcccagtcct tgggaatcct ccaaggaatg tcattctctt tgccaatcac tctattctta
     4321 tccatcgcag caatcaaaga aactttcatt tataacacag cctttttaac taatttacca
     4381 tctaatttct tatttctatc aaaaaacctt ttctcattat cctgcccgtt agttgaataa
     4441 gcaatttttt ctataagtta tattatcata ataccacaaa atcacaaatt ttcattcaat
     4501 tatcattttc aattgcattt tataaatcat tttgtatgta atttggtatg taagttaaaa
     4561 tgaaaaaagt agtccatttt gaagtacatt ttgtcatttt gtactccaaa actgaactac
     4621 ttagtataga ttactgttgc ttctttacta atttaggttt acttgttaag aaagattatt
     4681 tacgcaaatg ttcttcaaca atttgctgag atttatcagc tgtatcttcc acttcatctt
     4741 ttgctttttc taacttttga atatattgtt tccagcttga gttttcagca gttgcttcgt
     4801 ctaatttctt ttgtgtatct gttaactgag tttctacatt tgttttttct tgtgtcagtg
     4861 ttgcgatttg ttgattcaaa ccatttacag tattttgtaa ttctgaaagt tttgcttgtg
     4921 cttcttgtag tcttgctgtc caatctgcat tcagatgatt gatttcatca tttttttggt
     4981 tgatctcatc tagtttgaca ttgatttcat tttgtttgct ttgtagttgt gcatccttgc
     5041 tgactaattg actttctaag ttttgttttt ctgctgccga ttgatttttt tggttttcta
     5101 attcatttac ttttgtttgg tattcttgga catctgcctt tgaatcttgc aatgtctgtt
     5161 ctttttgtgc tagtcgagcg gtaagatctg taatcgcttg ctctttatct gtcaatgtat
     5221 gatcaagctg tgtcagattc tcattgattg tatcaatatc tgtctctcca ccccacaaaa
     5281 taatttcatt tgcgtaccgc gtgcctgtgt agccaattag acatagaaat gcaacgacaa
     5341 tcccaccagt aataaatttc tttttcattt gcatagctcc ttaatccaat acttttacat
     5401 gcta
//
LOCUS       DAZAID010000134         5339 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000134 DAZAID010000000
VERSION     DAZAID010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.137"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACG9BE_002688"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACG9BE_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACG9BE_002689"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACG9BE_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2090..3130
                     /locus_tag="ACG9BE_002690"
     CDS             2090..3130
                     /locus_tag="ACG9BE_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFN3033389.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3954..4286
                     /gene="traE"
                     /locus_tag="ACG9BE_002691"
     CDS             3954..4286
                     /gene="traE"
                     /locus_tag="ACG9BE_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFN3033390.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4905..5246
                     /locus_tag="ACG9BE_002692"
     CDS             4905..5246
                     /locus_tag="ACG9BE_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFN3033391.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1942 a          728 c          900 g         1769 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta attttaatgg
     1621 tagtacataa ttattatata tatctgacat tggtagtact tattgtattt tttagtagta
     1681 tataatctat tgttttctag cctaaagcct tgtcttataa gaataatata atatatacta
     1741 ctgaaataag gtagtatata ttactagtaa tggtagtatg ttttatatgc ataaggtagt
     1801 tcttaattta ttttttgttt tgacactggt agtacttaat tatctttaat tttaaaacta
     1861 aattttattc atacgttttt ctggtagtat ttaattataa ataatattta tttaaagtag
     1921 ttcttaatga ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt
     1981 aattaaaaaa cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag
     2041 agtaggtaat tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt
     2101 taaatttttt aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt
     2161 tattgtttct gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt
     2221 aaaagcaaga ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta
     2281 ttttacttat acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat
     2341 agcaatcaaa aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa
     2401 caaacagtta gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga
     2461 aaatgatatc tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc
     2521 agagggtatg aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc
     2581 tcaagagggt atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat
     2641 cataccagaa cttaataata atatattaga cactaataga cacaatatag acactgaaaa
     2701 agaccgccta caagatcaat tgttgttaga caattttgag acaattatga caaacgacag
     2761 cattgctacg tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta
     2821 cagtgaagct caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga
     2881 aagtgatatt tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga
     2941 attatacacg acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat
     3001 ccagaacctg atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa
     3061 agtatcaagt gaaaaacgtt tgaattatgc aagctcccca agcactatta cgaaagactg
     3121 gttagagtga aactttactg acaaagtgat tgttgcttca tggagtaaca ttaaaattaa
     3181 aaatttttta ttattctaat tatattacga atattacaaa ataatagcta ttttaataga
     3241 atttataagt taaaatatta tgataaaaat cacaaacgta gccttttacg tcataagtgt
     3301 taaaagaaat aattaatttc aaatatagaa aagggaacgt atgtttgcat actccttttt
     3361 tttgagttat aataatacgc gaagagagat aatttcatta tttaaacaag ctaaaaatct
     3421 tgatattact tcttttcagg caatacgaat attattcgct tacatttttt gtataaattt
     3481 aattgtctgc tatgcttgtg atgaattgat tttcgttttg cacttttgga tgataaattt
     3541 ggacaattga aaatacaaaa aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc
     3601 gtcagcgttc atacacggca ttaagatttg gcccttaatg cgttaacctt tcagtgttac
     3661 ggtattaccc gcaatggatt tagtataaca gatattcaaa agtgatacca ctttttttgg
     3721 ctgtgttaag gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat
     3781 aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt
     3841 ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa
     3901 aataacaaga aaaacttgtt aaaaatattt aaagaaattg ggtggtttat tttatgtcat
     3961 atacaggaat tttgagtctc aaagatattt gtcactacgg taaacggtgt accgctacag
     4021 agaaaatcac gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat
     4081 atataatcca gaaagacaaa gtctcagagg aaatgattta ttacattggg aaacaaaagc
     4141 aaataatttt aaaagatcct atccccttaa aagagctata tccaacaatc aaacatgttt
     4201 atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa
     4261 aagggatggg gcgtttgatc ggctgaattg atcattctca taacttgtat tttatcgttt
     4321 ttcgcttgct tgaaaatatt gtcgagactt ctaaaggaac ttagagtcac tagaaaaata
     4381 aggagtagac ttgatctatc aacaattccc ttcggggagt gacggggtag gttttaacga
     4441 ttggtaacgt aaaaaattgg tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg
     4501 aatttgtgta aataataagc gcagagtaaa caagagggaa tttagaagtt agacaagtta
     4561 gaatagcgtc tagattaatc atgagcagct actaagggat ctagactttg acgcaaactc
     4621 atggaataag gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg
     4681 ctgcagtaca tgtagatact tgcgaggacg ttgattattg gatcaatcga gcatttaccg
     4741 ctgtctacta gggtttctcg tttcggacgc cataaacgag tcgaaatttt ttatgtattt
     4801 ttaagtaaac gaaaaacgat aaaatatgaa caagtcctga ataaagtgaa aaaattgata
     4861 taaaacatag agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa aattatgatc
     4921 cgaatatacg attgggcaca catactatta aggtttcttt ccaacgttgg gattacaaag
     4981 gatttgtgac gtttagaagg ggcggaaatt gtaaaggatt agatgtttta gcattagacg
     5041 aggatgattt atacgatcaa acattgactg ataatccaat tggattcggc ttgctacctg
     5101 aagatgatga aggtaatgaa tggtttaaaa tgacgcttat gaatgataat ggagacgaat
     5161 tatctgttga agatacttgg tcatatttga gtgattatat tgtcagcttt gaaatcattg
     5221 aatttgtagc ggataaagaa gaataaaaac tggaggaata aattttacag ataaaggctt
     5281 agaaggagtc acgacgatta ataaaaaggt agtattacct taggcattga gtaaggaat
//
LOCUS       DAZAID010000135         5193 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000135 DAZAID010000000
VERSION     DAZAID010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.138"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..681
                     /locus_tag="ACG9BE_002693"
     CDS             <1..681
                     /locus_tag="ACG9BE_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN3033392.1"
                     /translation="RDVTELAKKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKY
                     IGQVMNSMIPDNSTIYVGAGTTILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILL
                     TGGEFSPITEEFIGEVAERSFESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNA
                     AKIKCIVADYSKLNKSDVYTFRKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDT
                     DNHDEPIH"
     gene            647..1600
                     /locus_tag="ACG9BE_002694"
     CDS             647..1600
                     /locus_tag="ACG9BE_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFN3033393.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(1998..2972)
                     /locus_tag="ACG9BE_002695"
     CDS             complement(1998..2972)
                     /locus_tag="ACG9BE_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN3033394.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3087..3521
                     /locus_tag="ACG9BE_002696"
     CDS             3087..3521
                     /locus_tag="ACG9BE_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN3033395.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(3646..4668)
                     /locus_tag="ACG9BE_002697"
     CDS             complement(3646..4668)
                     /locus_tag="ACG9BE_002697"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFN3033396.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5060..>5193
                     /locus_tag="ACG9BE_002698"
     CDS             5060..>5193
                     /locus_tag="ACG9BE_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HFN3033397.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     P"
BASE COUNT         1620 a         1000 c          930 g         1643 t
ORIGIN      
        1 cgagatgtca ctgaattagc aaaaaaaaat aaagtaaaaa agctatatgg tggcgttgaa
       61 cgaaaggaac cttataatcg agaactatcg actcaagaaa agattgatac gaatgtagcc
      121 gaaaaaaaat atattggtca agtcatgaac agtatgattc ccgataattc tactatttat
      181 gtcggtgctg gaacaacgat cctatatgca cttcctttca ttcaaaagaa aaatctattt
      241 gtaattacaa acagtttgat tgctttcaac tatttaaaga atcatacaag ttatcggatt
      301 ttacttactg gtggcgaatt ttctcctatt acagaagagt tcattgggga agttgcagaa
      361 agatcttttg aaagtttgaa tgtcgatatc tctttcgctg caacaaacgg catatttgat
      421 aacaatgtga caaccgctca atttattgag ggaaatattc agaacgcagc gctcaatgca
      481 gcaaaaatca aatgcattgt tgcagattat tcaaaattaa ataaatcaga cgtctatact
      541 tttagaaaat taaccgattt tgattatcta ataaccgata atcatttatc agaacaggat
      601 ttcaatcatt attcaaaata cttaacaata ttaaaggagc ccaaaaatga tactgacaat
      661 cacgatgaac ccatccattg attccattta ttttacaaaa cactttgaat taggtacgat
      721 gacacgcttt gattctccgg tacgctatgt aggcggcaag ggaatcaatt gcggacgagc
      781 ttctgctcta ctaggttcat cagttatttt aacagggttt ttgggcggtt attttggcag
      841 tatgattcaa gatatgctga aaaatgaaaa tttgtttgat ttgcattttt tacctattgc
      901 agaagaatca agaggtgccg tcacgatcat gcatgatgat gaaacgcaga cagagctagt
      961 ggaagctggt cctgaaatag cagatactac tatcaaaaaa ttaatgaatg atatcaaact
     1021 aatcagtaaa aataattacc tttcagtcat tacaataaat ggttctgtta acaatccaga
     1081 tccctttttg tatgctaaat tattacaata tatcaaagaa caaatagatc cttccaccta
     1141 tatattaatg gatatatctg gacaacatct ccgtgaagtc ttagcaagta agtataaacc
     1201 gatgttcatc aaacctaata tccatgagtt ttcagatatc atccatcaaa aaattactca
     1261 aaaaagcgac ataaaacacc atctaaaaac aaatccacta tttaaagata tcgatgttat
     1321 catgatttct tgcggctcgt atggtgcagt agtcaaagta aaagaagaat tttggaatgt
     1381 tgagatccct aagattccta tcattaatac aacaggctct ggggatgcca ctgttggagg
     1441 ttttgctcat tcattagaac agaattatcc tttaaaagaa tgtgtaaggt acgcaatggc
     1501 ttgcggcatg tcgaatgcac agcaatcaga agtcgggaaa gttattccag ctgatgtaaa
     1561 aaatttagca aaaagcatta aaatcacaga aatgaaataa atagtacaaa gtaaataatt
     1621 gtttatatga aatgagacac aaagagactg tgacaaaaaa cgtgtgtacc gacccccaaa
     1681 agttagattt tttggtctaa cttttgggag tcgatacatt gtcacagtcc ttttttcgaa
     1741 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatattcaac aaacatgaga
     1801 agctattttt tgaaattgct ccttgtgact cagagttaaa cggcaggctc acgacctctt
     1861 ggtataaacg gtgttcaaaa gcttgcatct acgctaatga gtccaaccaa gtcaaaaatg
     1921 aggagctttt ttggctgtcc ttattacttg atgcagggca gttttttcat aatctctttt
     1981 agattaaatc cttttggtta cacaaggttc tcttcaaaga atgttgccat tttttcaaat
     2041 ggaatgatgt ctgtctggtc gtatagatct gtatgggatg ctcctggaat gatcatgagt
     2101 tccttgttgt caccttttag ttctttgaaa gcgtcctccc caaagtaacg tgaatgagct
     2161 ttttccccgt gaatgactaa gactgcatga cggatttctg gaatataatg caatagtttt
     2221 gcattcataa gtgacaaccc tgatgtccta ttccaaccat cattggagtt taaagaacgg
     2281 acatgatatc cgcggtctgt tttataatac gcatgatagt ctttgacaaa ttgaggagca
     2341 tcttcaggca acggatcaac cacacctcca gcacgtgcgt agtccccttg tttgaaatca
     2401 atgatgcgtt gtgcatttaa ttcttcacgc atttcatatc gggcattttc atccataaca
     2461 tcgaagtagc catttgctgt tactcgagtc atgtcataca ttgtggaaac aactgttgct
     2521 ttgacccgcg tatcgatagc tgctgcattc aatgccatac ctccccatcc acaaatacca
     2581 atgattccga ttctctcagg gtttacattt ttttgtacag ataaaaagtc tactgccgct
     2641 tggaaatctt ccgtattgat atcaggcgaa gcaacatatc gaggtactcc tccgctttct
     2701 cctgtaaacg atggatcaaa cgcaatcgtt aaaaagcctc tttcagccat cgtctgcgca
     2761 tatagaccag acgattgttc cttcactgca ccaaatggcc cgctcacagc gattgccggc
     2821 aatttctttt cacttacttc ttttggttca tataaatcag cgactaatgt gataccaaaa
     2881 cgattcggga agctcacctt tcgatgattg acttgattgc tttttggaaa tactttgtcc
     2941 cattcttgtg tgatgtttaa ttctttattc atattctaga ctctcctttg atcaaaattg
     3001 acatttgttt ttgaatgtat tattatgata aaacttaaac ttgactttag gtcaaggtaa
     3061 aaattttatt ttaggaggat ttttttatgt attccattgg acaagtgtct gaaatgtttg
     3121 gattgcctgt ttctacttta cgttattacg acaaagaggg attatttcct gatatccagc
     3181 gtgtttctgg tattagaaaa ttttcagaga atgagttaga ggctttaaga gtcattgact
     3241 gtttgaaaaa atctggttta gaaataaaag acattaagca attcatggaa tggagcaaag
     3301 aaggcagtaa gacatttgag gttcgtaaag aactatttga aaaacaaaaa gaagtagtag
     3361 aaaaagaaat agcaaaatta gaaagagtac tcgctatgct aaactacaaa agctggtact
     3421 atgaagaagc aataaaagcc ggcaatgaag aagccgtatt gacaatgatt cctgatgatt
     3481 taccccaaca tgtaaaagaa gcttatgtac attctcattg aacaaacgga taactcactc
     3541 tccttctcta tgaacttcat gaaaacatga ataaacacct ctaactgatt gttagaagtg
     3601 tttatttttt cactcaagta tcctgcactt acaaaaatat cctttttacc gccatcctaa
     3661 ttctggagcg atatctttta cgattgtttc aagcaagtgc atgttatatt ctacccccaa
     3721 agtattagga atcgtgacaa gtactgtgtc tgcttctttg attgcttcat cttcagctag
     3781 ctccttgatt aattgatcag gttcaccagc aaaagttttt ccaaaaatcg ttgggtttcg
     3841 gtgataagca agatagccga cagcatcttg ttgcggacta ccgtcttgtc caaataagcg
     3901 tcgatccaaa tcagtggtga ttggctgaat cgagcgagta actaatgttc ttggttcaaa
     3961 atcatgacct gcttgcttcc acgcttcttt atacgcacgt aattggtttg cttgttggat
     4021 atggaatggc tcgtgggatt cgtaattgac taatgtagat gattgcagat tcattccatg
     4081 ttgtgctgcc cagattgccg tttgctgcga accagctccc caccagattc tttgtcgtaa
     4141 tccttctgaa taaggttcta tccgtaattt tcctgatcct tgtgggaaca ttggttgagg
     4201 atttggttct tcaaatggtt ttccttcaag taattccaag aattcaaggg tgcgttcccg
     4261 tgttacctct ttgatttctt cttcactgta atcataacca aagtatttcc agccatctag
     4321 aacttgttca ggtgaaccac gccccactcc tagttgcact cttccttgcg tgatgatatc
     4381 tgtcaatccg gcattttctg ccatataata agggttttca tatcgcatat caataagtcc
     4441 tgtaccgatt tcaatcttac ttgtctttgc gccgattgcc gctagcaaag gaaacggcga
     4501 cccaatctga ggggcaaaat gatgtacgcg gaaaaacgct ccgtccactc cgattttttc
     4561 tgcttccacc gctaaatcaa tcgattgcaa ataagcatct tgagctgatt tcacaagcga
     4621 tccttgctcc atccaatgtc caaagcttaa aaagccgatt tttttcaagg taatgacctc
     4681 ctttaaacct acaaaaatgg aaggtttttt atttttaata tgaactgatt ctactacttt
     4741 ttttcttaaa agacgaataa ttgttctcgc ctcacaatca agaaatgtat cggcttacta
     4801 cacccctact ttcttcatca tctgccgtgt cttaatctct tttaacagtc tgttacatcg
     4861 ttgatttttg actcaaaatg attgtttcta acatgtattt atggtaaaaa agacaagatt
     4921 agttctttgt acacatcgat ttatacgctg aacaaaaaga aatatataaa ctaagaaacc
     4981 cattgttttt tcgctccatc acatagagtt tactttagat aaacagtgac atttactaat
     5041 ccataacgga ggaaatcaaa tgaaaaaaat ctatttctta tgtacgggca attcatgccg
     5101 cagtcaaata gcagaaggct acgcgcgtaa atacttgtct ccttcaatgt ttgagataag
     5161 aagtgcgggg atcgagacac atggcttgaa tcc
//
LOCUS       DAZAID010000136         5151 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000136 DAZAID010000000
VERSION     DAZAID010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.139"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..388
                     /locus_tag="ACG9BE_002699"
     CDS             32..388
                     /locus_tag="ACG9BE_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HFN3033398.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="ACG9BE_002700"
     CDS             404..754
                     /locus_tag="ACG9BE_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HFN3033399.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="ACG9BE_002701"
     CDS             783..1562
                     /locus_tag="ACG9BE_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HFN3033400.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="ACG9BE_002702"
     CDS             1573..2235
                     /locus_tag="ACG9BE_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HFN3033401.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="ACG9BE_002703"
     CDS             2254..3360
                     /locus_tag="ACG9BE_002703"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFN3033402.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4441
                     /locus_tag="ACG9BE_002704"
     CDS             3350..4441
                     /locus_tag="ACG9BE_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFN3033403.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4454..>5151
                     /locus_tag="ACG9BE_002705"
     CDS             4454..>5151
                     /locus_tag="ACG9BE_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFN3033404.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIG"
BASE COUNT         1637 a          853 c         1240 g         1421 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacagggc gacatgtacc atgtctatca tccctataca gaaaaaatcg ctagacggga
     3661 aatcatcata cctaagggac ataatgtaga ttatgggaca ccggttgaag taatggttgc
     3721 tcaaggtgga ggaaaagtca tagaagctgg ctatgcaaat atgtgtacac cggaacatgt
     3781 ttccatgatg attacggaaa aaactgctgc gcttctatat attaaaagtc atcatgcagt
     3841 tcagaaaagt atgctgacgg tggcagaaat ggtagaagtt gccaaagctc atcgtttgcc
     3901 attgatcgtt gatgcagctg cggaagaaga tttattcaaa tatagcgaaa tgggcgttga
     3961 cttagtgatt tactctgggg caaaagcaat cgaaggtccg agtgccggac tggtcatagg
     4021 aaaaaagaag tatattcaat ggattcggct acagtctaaa ggtattggcc gagcaatgaa
     4081 aatcggtaaa gataatattt taggctttac ccaggcacta gaagattatt taaaaaatgg
     4141 gagcgaatcc ggtgactcca tgaagcaacg attagcgcct tttgttaacg caatcaatca
     4201 acttcctgac ctttcagcga aaatagttca agatggtgcc ggcagagata tttatcgagc
     4261 aagtgtaacg attaatggcg ataaaagtgc gaaagaagtc attcaggaat taaaagcacg
     4321 aaatccagca gtctacaccc gagaatatca agcaaataac ggaatcattg agttcgacat
     4381 tcgttccgtc agccaaaacg aaatggaaaa aattataaaa agattgagag aaatcatgta
     4441 ggaggctttt aaattgtcag ttatcccaaa ttattataaa aatcgtgtat gtttaaatgt
     4501 attagctcat tcagttgaaa atgcaaaagc atgctatgaa gcagcggaag ggcatgtcgt
     4561 actaggtgtg ctatcgaaaa attatgctag cgatgaagca gcaatcgaag atatgaaaag
     4621 gtaccaagac gcaaccaata atgcgttgtc agttggatta ggcgctggag atccaaacca
     4681 aagtcagatg gtttcgcgtt tatctggggt tttacaacca caacatgtca atcaagtatt
     4741 tactggcgtg gggacttctc gtgctttatt gggccaaaat gatacagtca tcaatggatt
     4801 ggtttcacca acaggtaaga tcggctatgt caatattgct acaggtccgt taagttcaca
     4861 agcgccagca gctgaagtgc ctattgaaac tgccattcaa atgctgcaag acatgggagg
     4921 tacttcaatc aagtatttcc cgatgaaagg cttagcacat agggaagaat ttaaagcagt
     4981 tgcggaagct tgcgcaaaat atgaattcta tttagagcca acaggaggaa ttgatctttc
     5041 aaactttgaa gaaattctga aaattgcaat cgatgcaggt gtaaaaaaag tgattcctca
     5101 tgtttatagt tcaattatag ataaagaaac aggagatacg cgtataggag a
//
LOCUS       DAZAID010000137         5128 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000137 DAZAID010000000
VERSION     DAZAID010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.140"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..457)
                     /locus_tag="ACG9BE_002706"
     CDS             complement(<1..457)
                     /locus_tag="ACG9BE_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN3033405.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(470..1801)
                     /locus_tag="ACG9BE_002707"
     CDS             complement(470..1801)
                     /locus_tag="ACG9BE_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFN3033406.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1812..3254)
                     /locus_tag="ACG9BE_002708"
     CDS             complement(1812..3254)
                     /locus_tag="ACG9BE_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3033407.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3241..4005)
                     /locus_tag="ACG9BE_002709"
     CDS             complement(3241..4005)
                     /locus_tag="ACG9BE_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFN3033408.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4109..4984)
                     /locus_tag="ACG9BE_002710"
     CDS             complement(4109..4984)
                     /locus_tag="ACG9BE_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN3033409.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         1554 a         1085 c          753 g         1736 t
ORIGIN      
        1 aaaattcaaa acaactatct ttaatcttta cttcttggtt ttgttttaat aaatttcctt
       61 caaaaatgcc tggagaaact acaatgatca ccgtatcttt ttgtgcttgc tccaacttct
      121 tcaaatctat aatagctaac agtgtccctt cttttacctc ctggttactt tcaacccgta
      181 tctgaaacgg acttccttcg agttcaactg tatctaaacc catatggatc agtatttttc
      241 tgccagattc actttcgatc gttatggcat gtcgggtagg aaatatatta tcgattattc
      301 ctttaactgg cgcatggata tatccatcat gattcgttat agcgtatcca actcctatca
      361 tttttgaaga aaagacctcg tcttttactg ctgttaatgg aataatttga ccactagcta
      421 cagcttttag aatgtttctt gttttaaaga aatgcacttt ttatcacctc tactttaatg
      481 ctggccaata ttttttgttt actttgatca tttcatctag tatttttttt gcgactctgg
      541 catttggtac ggtttgattc aatgtaaatg cttgtaaagc tttttgataa gaaccttcaa
      601 agtaagcatc tactagtaac ttctctactg caacttgtgc ctccatcaat cctttatgaa
      661 aatcactgat tggttttctt agtgtaattg gctccacacc agttgcacca acatatgccg
      721 gtatttcaac aactgcatct gaccgtagat tcggaatcgc cccttgatta ggaacgatta
      781 gcataaagcg acgacgatca tcattcaaca aggaaatagc tatatcgacg atatattgtc
      841 catgctcccc aaaattaaag ttcaatactt cttccacaat accgcggcgt attttatcag
      901 ccatttcttt ggtatttttc tctcgtccat ccatgaccat attagcacgt gtatactctt
      961 tgtcagtatg ctcaacgacc atatcaggat ataagtaata ctctaagtaa ttattcggga
     1021 tattacttgg aaaatttttc gtgatgactg acatcatttg aaaggttttt tgccaagttt
     1081 tatctcctat attaaaatcg gcgacttgca tttcttttgt tattagtttt tcaataatct
     1141 ctggcataat ttctcgttgc aattctttat cataaatcga tgtataccag ccaaagtggt
     1201 ttaaaccata atacgtaacg atccaattct tacgatcata accgtaattc actgcaatcg
     1261 tttcttcaat tgaaatagtc atatcacagg cattaatcat ttttacattc ggatatttac
     1321 gacgaacagc ttcagaaacg atcgactctg gatttgtata attcaaaatc catgctttgg
     1381 gggcatagtc ttgtatatga tcgacaattt ccaataaacc tttgattgag cgcatcccat
     1441 aagcaaatcc tccgagtcca cacgtttctt gtcctactaa tccatgttta agtggaatct
     1501 tttcgtcctt ttctcgcatc tccaatcctc caacacgaat ttgtgagaaa acaaaatcgc
     1561 atcctgtaaa agcaagcttt ggatcatccg ttgcacgaat gcgaatattt gaaaaacctt
     1621 ttcttttcag cataaattta acaatcaagt acatatccat atttctttct tcatcaatat
     1681 catataaacg tatttcagat aatggcaacc gtttttcatt gttcaaaatg gcttgaacga
     1741 ttcctggtgt atacgtactc ccgcctccag ctatagtgat aatctgtttt ttcatcatca
     1801 tcttttcctt tctattcttc taataattta ttgaattctg ccataacata agtcacttct
     1861 aagccgatga taatttgata atttttttca gttggtttaa tgatcccgct tatccctaat
     1921 tgcttgattc gctgttcctc gaccatggtg ggatccacta cctctacccg taggcgtgta
     1981 ttacaactat agacatccag gatattggct tttcctccaa acgcttgtag catttttgtt
     2041 gccatatatt cataattttt acttgctaac cctaactctg tttcttctaa ggttacttca
     2101 ttgccaaact ctgccttagc ttcacgtcct aatgtctgaa tgtttctctt attgatcaaa
     2161 taataaaaag taacgtaata caaggcaaaa aagaaaattc cgattggaag aatcaaaata
     2221 gcattggtag cgattcgaaa attgatgaga taatcaacaa tacatgctcc gaatgtaaaa
     2281 ccaattcgta tattgaataa gtaacagaca atccctgcta aaccggcaaa aagtgcatgg
     2341 atcacgtaca acataggaga aagaaacatg aaactaaatt caacaggctc agtgatattt
     2401 gcaataaatg aagttcctgc accgcttccc atgattcctt tgaccatctc tttattttct
     2461 ttataagctg ctttatagat agctagacat atagctggta taccaaacat cattactaca
     2521 aaaaaaactg ataaaaacag tccagcctga ggatctccgt tgataaagcg tgtcatctct
     2581 cctctgaaaa cttccttgct aggagatgta tagcttccta aatcgtaata aatatacgta
     2641 ttcaacacat ggtgcagtcc aaaggggatc agcaaacgat tcaaaaacat aaatatcccc
     2701 acacctaatg ctcccatatc aacgagcagt tttgcaaaat tatcaatccc aatttggatg
     2761 agcggccaga taaagcccaa aaaaacagct gcgattgttt gtagaatcat caccatagtc
     2821 aatgggaatt tttcgcccgc aaaataagaa aaaactactg gtaatttttg ttctttaaag
     2881 cgattatacg tccatgccgc tactaaaccg gaaataattc caccaaaaat ccccatattc
     2941 acttctgggt ccataatttc taagcctttt ttcaaggtag caatcgctaa aaaaccagta
     3001 agtgcaggta ttcctttgtc cttatttttg acaaaaccga ttgcaactcc tatagcaaat
     3061 aagatatcca agtttccaaa aacggcattt ccagctgcac caattactgg gatgttcagc
     3121 atatcgtcag ctgataaacg catgatcaat cccgctaatg gcatcgctgc aactggaagt
     3181 agcatcgctc ttccaagctt ctgcacattt gatttaacat tcatttttat tcctcctcta
     3241 ttaaatatcc tcatatgtta ctaactcaat caaaggatca tttttcactt tttgagcaaa
     3301 tgctgaatga attaaaaatt cgacttggtg ggtacgagga tacacaaaac ttgaaccatt
     3361 taacaattcc tgatccaaat acgctgcgtg acacatgact tcgatcgttt tatcttctct
     3421 caatatcatg agtaaacttg ccaaatattc ttccacactc gtattgattt gtcgtttttt
     3481 ttcatcagtt tctcctacga catcgaaaca aggctcaaaa taatcgacat gacgaacttc
     3541 atcttttcgt tcaaagttgc tccgaaccgg caaatcatat ttttttgcta atgtaacaac
     3601 tacctcctga ttttcatcca aagtatgagt atgatgatga ctgtctaaat gagttggtat
     3661 gatccccgca tgataaattt tttgaatctg agcattccat tcctgataca gttgatcttt
     3721 gtcaatgtta aatggatgct gataataaga taaggatctg aactctccgc tttcgataag
     3781 agtatcgaca gttttgagga gtggtttgcc acacgtcaat gttaaatgga ctcctacacc
     3841 tagcctaggc atttcctttc ttagtttcac agcatgctcg aaacccggca tatttgccat
     3901 caatgtagtt gatgtcaaaa ttcctcgctg ataagcatcc ataatcccat aattcacacc
     3961 atggctatag ccaaaatcat ccgaattaaa aatcactttt cccatttcat tctccttctt
     4021 ttttctacta aaataggcgc aaccaaataa tcatagtcca tttactatgt gtcatttggt
     4081 tgcgcctata gatagttacg cccttatttt aatagattct tgctagatga attgccaaat
     4141 agatgatttc attttcttga atggcaagtt gaaattgctg ttcgatgata tctttaatct
     4201 ttagtgctgt acaatacgct ttgctatgtt taaatgtttc agtaaaaaaa ccaatatttt
     4261 ctactggcgt ttcttcctga taaatcaagc gtttaaccag gaatttgata tgaaccatta
     4321 agcgatgata agtcagtgat tctttatcat aaacgtttga ataggatttt tctagatggg
     4381 acaaaatcgt ggtcgtcatt tctactattt ttaagctctt taaactatta ggctctccta
     4441 gctcagcatt aatgatatgc atagtgatga atcctacttc atttgaccct aactcggttt
     4501 gatatcgatg attgattcgc tcgaccactt ctactgctat atcatattca tttggataaa
     4561 ggaatctcat ttctggcaac ataattgctg tgacatctct atcttctctt tttcgttgaa
     4621 tagcaaaata aatatgatct gtcaatgtaa tatagatatt tgaatctaat ggagaggaga
     4681 tcctagctgt tatcagttca atcacttctt ccgcaaaatc cataaattcg attggaatct
     4741 gcgagactat ctcactgaat cgtttgatct tttctttaga atctaataca aataatttgt
     4801 cagcatcctc aacatcaaat ggcttactca ctttagcaga aaagccaatc cctttcccca
     4861 tcgcaatttt ttctattcca tcgtcatcga tcaatacagc attatgattc agctttttca
     4921 tcacatacac cgttaattca cctcctctat atgataaaac aaaacaactt gcagactcct
     4981 tcatacatat ttttgtctcc aaacaatttt taaagacaaa aaacgaccta agaaagaacc
     5041 ctttcttagg tcgtgcctaa ttgaatagta gcaccctaca taatttacaa aaatagttta
     5101 acacataata aaagcgttat caatatct
//
LOCUS       DAZAID010000138         5080 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000138 DAZAID010000000
VERSION     DAZAID010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.141"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACG9BE_002711"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACG9BE_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFN3033410.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="ACG9BE_002712"
     CDS             complement(2066..3064)
                     /locus_tag="ACG9BE_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFN3033411.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="ACG9BE_002713"
     CDS             complement(3290..3943)
                     /locus_tag="ACG9BE_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFN3033412.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACG9BE_002714"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACG9BE_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFN3033413.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1506 a         1041 c          771 g         1762 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 aaaatgaaag ttatctaagg ttaatataca gtaaaaccag cattctcgct ttttttatca
     4861 tttaaaaatt agagttttca aatctaattc aaattttcta aacatatgaa acagcgtata
     4921 aaactgtact attattttat ctcaaaagct aaatgaaacc gatttcttaa gattttttat
     4981 gttttttatt tctttaatag taatgttttc tgtcatttta aataatactg ttctttatta
     5041 taacgcttat tttctctgtt atataagcgt agtattttaa
//
LOCUS       DAZAID010000139         5059 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000139 DAZAID010000000
VERSION     DAZAID010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.142"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACG9BE_002715"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACG9BE_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFN3033414.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACG9BE_002716"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACG9BE_002716"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFN3033415.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACG9BE_002717"
     CDS             complement(2169..2906)
                     /locus_tag="ACG9BE_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFN3033416.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ACG9BE_002718"
     CDS             complement(2997..3524)
                     /locus_tag="ACG9BE_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033417.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACG9BE_002719"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACG9BE_002719"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFN3033418.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACG9BE_002720"
     CDS             complement(4401..4886)
                     /locus_tag="ACG9BE_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFN3033419.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
BASE COUNT         1342 a         1128 c          864 g         1725 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttc
//
LOCUS       DAZAID010000140         5041 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000140 DAZAID010000000
VERSION     DAZAID010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.143"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..563
                     /gene="mgsA"
                     /locus_tag="ACG9BE_002721"
     CDS             141..563
                     /gene="mgsA"
                     /locus_tag="ACG9BE_002721"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFN3033420.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      750..848
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            886..1422
                     /locus_tag="ACG9BE_002722"
     CDS             886..1422
                     /locus_tag="ACG9BE_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFN3033421.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKNLRSLAIHAK"
     gene            complement(1462..3225)
                     /locus_tag="ACG9BE_002723"
     CDS             complement(1462..3225)
                     /locus_tag="ACG9BE_002723"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3033422.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3228..4943)
                     /locus_tag="ACG9BE_002724"
     CDS             complement(3228..4943)
                     /locus_tag="ACG9BE_002724"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3033423.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         1535 a          980 c         1015 g         1511 t
ORIGIN      
        1 ttcaatgtca tgttgaacaa gaattttttc ttttttttgt caatgtgcta gttttaagct
       61 ttaataaata tccataagaa ataaaaactg atacactgga gtaaataagt atgttaacaa
      121 tatcaaaagg agtgtctctt atgaaaatcg ctttgatcgc tcatgaccga aaaaaaccta
      181 tgatggtgaa attagcaaca gcgtataaaa caatattgga aaaacatgaa ctttttgcta
      241 ccgggacgac tgggcagagg atcacggaag caaccggctt gccgatccat cggtttaaat
      301 ccggaccgtt aggcggggat cagcaaattg gagccatgat ttcggaggat cagttagatt
      361 tggtgatttt tcttcgagac ccgctggctg cacagcccca tgaaccagat gtaacagcac
      421 ttatccgttt atctgatgtc tatgagattc cgttagctac gaatatcgga acagctgagg
      481 tgctactaag aggactggaa gccggcttcc tagattttcg aagaatcgtc catgaaatag
      541 acaagcatcc actttcattt tgatggataa gcgtatggaa aaataacgtt taatgttcgc
      601 ctatatgtag ctataaaagc taaacaaact gataatatgc gatataatac gaacgtattg
      661 gataatataa acaataatat tagtttttcg tatttgcagt aaatgtgaaa atatgctata
      721 gtttctgtgg gaaaagatcc caaaaataat acagagtaga aaataaagcg ttaagtgcta
      781 agaggatggg atgttgcctc ttggacgaag gacgtaatgt tcgcggtttt attttcgcat
      841 tcgctgcatg atacgatgta gcagctctta aaggagatgc taaaaatgaa gaaacaccgg
      901 ttagacgcac gtatgattgc tattatgggc ttattgattg cattgatggt cactttgtcc
      961 cgactggtag caatcgagac cccatttatt aaaatcagtg tcacatttat tccacaagtg
     1021 atcatgggca ttctttttgg tcctttttgg tcagggatag gcgcagtttt agctgattta
     1081 gtaggaatgg ccctcttctc gaaatctgca ttctttattg gatttacatt gaatgcattt
     1141 attgaaggag caatctacgg attctttttc tatcgaaaag agatcacttg gaaaaatgcc
     1201 attctggcta cgcttagtgt cacgctgatc atcaacttat ttttgacacc attatggctt
     1261 gcgttgatgt accatgtgcc attgttcagc tgggtcgttt gggcgccccg cctgttaaaa
     1321 acagtaatat ggttaccgat tcaatcaatt gccatttatt acgtaggacg tagtattcct
     1381 tacaaaaaaa atttaagaag tttagcgatc cacgctaaat aaaaaacaag cagtcttccc
     1441 atcccagaag actgcttgtt tttattgtgc tgtgaattgt gcttgataga gccgtcggta
     1501 ataaccatct ttttgaagca gctcatcatg ggtgcccatt tcgacaatag ctccctgatc
     1561 catcactaat atctggtccg catttttgat tgtggacagg cgatgggcaa tcacaaaact
     1621 ggtttttcct tccatcattg ctaagaaggc ttgttggatt tttttctctg tcaatgtatc
     1681 gactgagctg gtagcttcat ctaagatcaa cataggggga ttactgatca tcgttcgagc
     1741 aatcgtcaac agttggcgtt gtccatctga tatctttaac ccttgaccgc caatcttagt
     1801 atctaatttt tccggcaaac gttctacaaa ttcatacata taagaggctt ttagggcatc
     1861 attgatttct gcgtcacttg cttccggatt cccaaaacga agattttctc gcaatgtact
     1921 gtcaaatagc caagtatctt gtaacaccat tccaaaactt ttacgtaatg tatcacgttg
     1981 gatagagcgg atgtcatggt catcgactgt aattgttcct tttgttgtgt cataaaaacg
     2041 catcagcaaa ttaacaagtg tggattttcc ggcccctgtt tttcctacta tagcaatggt
     2101 ttgacctgga taagcagtaa aattgaaatc ttcgatcaaa cgctgcttgg ggtcataaga
     2161 aaaagaaaca tgatcaaaag agacttttcc tttgatattt ttgggttcca aggcataggc
     2221 atctggcaaa tcaggctttt cttctggctg atcgatcaaa tcaaatgctc gttctagacc
     2281 agaaaaagcg gtttgcagtt gtgtcgtgat tccagaaagt tcgataaatg gtttagaaaa
     2341 ctgacttgag tagatcgtaa agctggaaat cacaccaaca gtgattgacg aaccactctt
     2401 caatgccaat aggccgccaa ctaaaccaat cgataaataa gcaaggtgat ctacaaaacg
     2461 agaagacggg tttgtcaagg aagaagaaaa ttgtgcccgt tgtccttttt catatagatc
     2521 agcatttagc agttcaaatt gctgttgact agcttcttct tgctgaaaag ctttcacgat
     2581 tttctggtta ccgattcgtt cggtcacaaa gcccgagata tccccaataa tcctttgctg
     2641 tctatcgaaa tttgtttgtg aagcacgagc aacgatccaa ctcacaagga aaataattgg
     2701 tgtagaacaa agaacgacca atgtcagaag tggacttaga cgcatcataa aaataaacga
     2761 aagaatgatg attgccactc cagaaaataa ttgattgaag gccgcagaaa ttgcaatgga
     2821 aatattgtcc atatcatttg taaatcgact gacgatattt ccgtgggagt tttgatcata
     2881 ataacgaagt ggcaatcgat tcagatgagc aaatgcatct tttcttaatt ttgcaacgga
     2941 taagtaagct aaccgattgc ttaacacttg gatgagccat tgactgacta cgttgattaa
     3001 aacgatacca ccgaatagaa taagtaaatg gaacagtcga ttgaagcgga cctgtccttc
     3061 tccaatcatt tgatcgatac tttgaccaat ttgtaacgtc atcacaactg ttgcaatccc
     3121 attgatgatt cctagtatca cagctagcca aatgtctttt gcataggcag taaggtaagg
     3181 aatgaatcgg cggagcgtct gcaaggataa tttttttact ttcataacta ttcttcctcc
     3241 tgtgatgcga cgatttcctg atattctgct gattgagcaa gtaattcttc atgggtaccc
     3301 tgagcagcaa tcttaccttg atccatcact aagatatgat gtgcttcttg aatagagcgg
     3361 acacgttggg agataataat cactgtactg ccaagataag tctgtagcgc cttacgcaaa
     3421 tccaagtctg tttgataatc caacgcactt aaagagtcat ctaaaatcag taatgctggc
     3481 ttggcaatca aagctcttgc aatcgtcagt cgctgttttt gtccgccaga gaaatttttc
     3541 ccattttcca tgacttttgt atctaaacca tttggaagct gacggacaaa ttcttctgcc
     3601 tgagcgattt tcagagcttc ccaacattct ttttctgtgg cgtctttctt accccattga
     3661 aggttttcac gaatagtccc ggtaaatagt acagaagttt ggggaacaag agcaatttgc
     3721 tgacggagat gatccagctg ccaagaacga acatccattc cgtccattat aactgtaccg
     3781 gaactgacat catagaaacg cggaatcaat tgtgtcaaag tcgttttccc gcttccggtt
     3841 ggaccaatga ttccaaggac tgtatttcgc ttcagctgaa atgtaatatt ttggagagaa
     3901 agaccagaat caggagtata acggaagtca acatggtcaa aagcgataat tgcttcagaa
     3961 gaaaaatcgg gagtagtact gacctcagct tcaagaatcg aattttctgt gtctagcacc
     4021 tctttcaccc gatttcctga agcttcggca cgtgtgaaga tcacgacgag gtttgaaaca
     4081 acgatcagtg ccaaaagcat ttgattcata taattgatca aagcaagaac ttgtccttgt
     4141 tgcaagctac caatatttac tttgatcccg ccaactgtca gcaaacagat gatcccgaca
     4201 ttcatgatca aggttgtagc aggtgataat agggcagaga tattggaaac acgtaaataa
     4261 ttgttcgcta gatcatttgt gcttttatcg aacttctctg tttctttttt tgtctgcgca
     4321 aatgcgcgga tgacacgaac accgctaaga ttctgactaa cgtgttcatt gagctgatct
     4381 agtttttctt gcactttttg gtagagagga acggtttttt tgatgataaa aaataaaatg
     4441 attgagaaaa taggcaataa tgctaagaaa aacagaccta cttcccagtc gatcacaaaa
     4501 gccatgacca ctgacccgat acttaaaaac ggtgctctga tcaataaacg aatgaccatt
     4561 gctaaagcta gctgaagctg attgatgtcg tttgtcattc gggtgatcaa ggtatctgtt
     4621 ccaaaatgat tcaactcgtt gtgagagaaa gtattgattt ttttgattag ctgattacgt
     4681 aactctgtac caaatccttg tgaagctact gaagcatagt attggcaaat gatagcacaa
     4741 atcagaccaa tcacagacat agcgaccatc cataagccca tttcaatgat ttttcctcgg
     4801 tcattcattt ttagtccatt atcgatcaag gaagccatca ttaaaggcag gacaagttcg
     4861 aagacagctt caagaaattt gaagaacggg cccaaaatga tttgcttacg atagtctttt
     4921 gcataacgta ataactgaat catatgattc ctcctattat ttatttgtcc acaattgaaa
     4981 aaacaagtac taccacattg tagcttagga aatttagaat gaaaagagaa caagcctttt
     5041 c
//
LOCUS       DAZAID010000141         5013 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000141 DAZAID010000000
VERSION     DAZAID010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.144"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..262
                     /locus_tag="ACG9BE_002725"
     CDS             50..262
                     /locus_tag="ACG9BE_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033424.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            complement(239..1849)
                     /locus_tag="ACG9BE_002726"
     CDS             complement(239..1849)
                     /locus_tag="ACG9BE_002726"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFN3033425.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(2071..2316)
                     /locus_tag="ACG9BE_002727"
     CDS             complement(2071..2316)
                     /locus_tag="ACG9BE_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033426.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(3042..4382)
                     /gene="glnA"
                     /locus_tag="ACG9BE_002728"
     CDS             complement(3042..4382)
                     /gene="glnA"
                     /locus_tag="ACG9BE_002728"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFN3033427.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4410..4802)
                     /locus_tag="ACG9BE_002729"
     CDS             complement(4410..4802)
                     /locus_tag="ACG9BE_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN3033428.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1490 a         1073 c          811 g         1639 t
ORIGIN      
        1 ccaacgaccc tattttacca aacatttcgg tagttttcta ctaaaatcaa tgaaaatgaa
       61 aattttcaag aagttttttg caaaaaataa aatatccaat agcgctttaa tcgaaaaaag
      121 tcttttgctt tctatctttt ttcctacaaa taataacaaa tcagaaaaag agaaaagtga
      181 caagaaaatt gcttatcccc ttctcttgct acttttctct ttctatcaag ttcaaaaatt
      241 attttgtttc gtgatctgct aatgatgctc cgacaaatgc tttgattaat ttttgcgggc
      301 gatttggacg agaaatcaat tctgggtgga attggcatcc cacaaagaat tttttctctg
      361 gcagctctac gatttctact aaacggttat ctggtgagac acctgaaaag actagccctt
      421 gttcttcaaa caattgacga tatttgttat tgaactcata gcggtgacgg tgacgttctt
      481 gcacaacatc ggctccatca tatgcttcag ccgtcttcgt tccttttttg attttacatg
      541 ggtataatcc aaggcgtaaa gttccaccca aattctcgat gttttcctga tcagccatta
      601 aatcaataat attgtttgct gtatctggat tcgtttcagc tgatgacgca tcttctaatc
      661 caacgacgtt acgtgcaaat tcgacacaag ccatctgcat accaagacaa atacctaaaa
      721 acggaacgtc attttcgcga gcataacgaa tcgcttcgat tttcccttct actccgcgat
      781 caccaaatcc accaggaacc aagattccat ctgcgtcttt caaacgttct tctacatttt
      841 ctctcgttaa ttcttgtgct ttgatccaat cgatctcgat gtctgaatta taggcgaaac
      901 cagaatgttt caatgcttca actactgaga tatatgcatc tggtaactcg acatatttac
      961 cgacaagcgc aatccgtact tttttcttca agttcaagac acgttcttct aattctttcc
     1021 attctgtcat gtctgcttct ggtgtatcta gttttaaatg gtcgcaaaca atttgatcca
     1081 ttccttgtgc ttgtaaaact aatgggatgg agtaaagtgt atctacatct ggcgactcga
     1141 taactgcttc tggttcgaca tcacaaaatt gtgccaattt gtttttcacg ctttgagaaa
     1201 caggttgctc tgtacgtacc accaaaatat tcggttggat acctaaacca cgtagctctt
     1261 ttacactgtg ctgcgttggt ttagttttca tttctccagc agctttcaag taaggaatca
     1321 atgttgtatg gatatacata acattatctg taccgacatc tgctttcatt tgacgaagtg
     1381 cttcaaggaa tggcaaagat tctatatccc ctactgttcc ccctacttct gtaatgatca
     1441 catctgaatc agtcatttta gctgctcgca tgattttttc tttgatctca tttgtaatat
     1501 gaggaataac ttgaacagtt gctcccaagt attcgccctt acgttcttta cgcaaaactt
     1561 cagaatagat ttttcctgtc gtcacgttgg agtatttgtt caaattgatg tcaataaaac
     1621 gttcgtagtg gcccagatcc aaatctgttt cagcaccatc atctgttacg aatacttctc
     1681 cgtgctgata tgggctcatc gtccccggat ccacgttgat gtatggatca aatttttgga
     1741 tagttacttt taagccgcgg tttttcaata agcgtcctaa agaagctgca acaatccctt
     1801 ttccgattga tgaaacgaca ccgccggtca caaaaatata ttttgtcata agttcaaaaa
     1861 ctccttttta atttatgtgc aggaaaagtc tcagacaata aacaaagctc cctattcttc
     1921 aataagaata gggagccgaa atttctgtaa acttctgacc ctctaaaagg gtgcccaagg
     1981 tgtataatac aagctgacta agggaaagtc aagtactcaa atgaatacta tccgcttaca
     2041 attttttctt tttcgatatc ctttgagaaa ttatttctta agtcctttat gagtgcaaaa
     2101 aaaatttaac ccacataata agcttgatat aaacatagct aacgttaaac ctaagcaaat
     2161 cagaaaatcg ttactgaaaa attttaagtg aaataataga taaaaaaata aaacaaacat
     2221 tatgatagat aatatcccac ctaacaaaga actacttttt cctacttgca gcaacagttt
     2281 atatagctta tcgtagaaac aatctttttc tttcaccctt atttacctcc tttacgtaaa
     2341 aaacgtagtc cacagttctc ggatttttgg catatctttt ctattaaata atatcaaaaa
     2401 caaaacaatt ccactaattt tttcaaagat gacctttttt tcaacaaaag tgccttgtag
     2461 ggattcattg aaggtaacga atcacataag taaaaaataa ggagatgcga caaaagtcgt
     2521 gcctccttat ttctaaataa acggtgttct cctgccaact cctcggtcac aagtcacaat
     2581 atccatcaaa catgagaagc tgtttttgaa attatttctt gtgattcgga gttaaatggt
     2641 aggctcacaa cctctggata aacggtattc aaaagccagt ttctcggtat taagccaaat
     2701 ttatgcaaaa atgtagaaag ccattttcac aaaatttttc ttaatactcg aaactaaacg
     2761 tctttttcaa aacctcttgg taaacggtgt tcaaaagctt gcatctgcgg taataagttc
     2821 agccaattca aaaacaagaa aagctgtttt tgcttggcta ttcttattac ttgttgcagg
     2881 gcagcttttt cacaacctct ccactaaacg gtgttcaatc agctgaccct cggtattagc
     2941 tcaaaaaacg gcaaaatatg aggaactatt tttccgttag tttctctaat actcaggtca
     3001 aaacgctgat ttcacagcct ctaattcacg gtattcctgt tttaataaag ttctagatat
     3061 tgttctcttt cccactcgga aacagtttgt ctgaatgccg cccattcgag acgttttgct
     3121 tcaacaaaat ttgagaagat atgttgtcct aatgcgtcaa tcattacatt atccttacgc
     3181 aattctttga tagcattatg gatagttgaa ggtaagtcct ggatatgtgc agcttctctt
     3241 tcttcctcat ccattacata aatgttacgg tcaacagctg ctggcggtgt gatctgatta
     3301 cggataccgt ctagtcctgc ttgcaacaat actgccattg ccaagtatgg gtttgcagat
     3361 ggatcaactg accgcaattc caaacgtgtg gataaaccgc gagattctgg tacacgtact
     3421 aatggcgaac gattacggcc actccatgcg acataaacag gcgcttcata acctggaacg
     3481 agacgtttat aagaattgac tgttggattg caaacggctg tgtaggcacg agcatgtttc
     3541 agcaaaccac ctaagaaatg ataagctgtt tcacttagtt ccaattcacc gtctttatca
     3601 tagaaaacat ttccttcatc attgaacaaa gacatattgc aatgcatacc agaaccattg
     3661 atcccaaata acggtttcgg catgaaagtc gcgtgaagtc catgcttgcg tgcaatcgtt
     3721 ttgacaacga gcttgaaggt ttggatatta tcacaagcat ctaccacatc tgcatattta
     3781 aagtcaattt catgctgtcc tggcgctact tcatgatgtg aagcttccac ttcaaagcct
     3841 agactttcta attccaacac gatgtcacgg cggcaatttt cacctaaatc tgttggtgca
     3901 aaatcaaaat aaccaccttt atcattcaag gtcgttgtga tcccaccgtc ttcgtctaat
     3961 ttaaataaga agaattctgg ttcaggtcct aaattgaaag aggtaaaacc aaggtcgcgc
     4021 atatctttca atgctcgttt caaattcccg cgaggatcgc cggcaaatgg tgtcccatcc
     4081 ggattataga tatcacaaat taaacgagca acttttccat gagcactttc ccacgggaag
     4141 atcatccaag tggagagatc tggatacaaa tacatatcgc tttcttcaat tcgaacaaat
     4201 ccttcaatcg aagaaccgtc aaacatcatt ttattttcta atactttgtc gatctggctc
     4261 actggaactt ctacattttt gatcgtacct aagatgtctg tgaacatcaa tcgtaaaaat
     4321 cgtacgtttt cttcctcaat gattcgtttg atttcttcac ctgttatctg tttctttacc
     4381 atggtatccc tcttcctttt ttaatttatt cagtcaaaga attgtctttg caatccttgc
     4441 gactgataat gacctgtttt gcttaaccca ccttgcgcca aaatctcatc acgaaaaatt
     4501 tgtcgaacgt catgatctgt caacggcttc ttgttttttg cttcttgttt tttcgcctgt
     4561 gccatttcgt atacacgttt gatcccagcc atattcaatc cctctgaaag atagtcttta
     4621 atctcaagca acatatcaat atcattcagc gaatacatac gtcggtttcc ttcgcttcgt
     4681 tccggatgaa ctaaatcttg ttcttcataa tagcgaattt gccgagcaga caaatcagtc
     4741 agcttcataa ctgtaccaat tggaaatacg gacatagatc ggcgcaattc cttttctccc
     4801 atcagacgtc ccctttcgtt ttataagttg gctaaagtgt accaattaaa aaaaatattg
     4861 tcaactgcat atgttaggtt ttctaacatg agatctctat tttttataaa ataacaaaaa
     4921 aataagaaca caaaaaaggt atctagttga ttttcgtacc agataccttt tattttttat
     4981 tatactgtac tatcctttat tagtgttttt att
//
LOCUS       DAZAID010000142         4963 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000142 DAZAID010000000
VERSION     DAZAID010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.145"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="ACG9BE_002730"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="ACG9BE_002730"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFN3033429.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="ACG9BE_002731"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="ACG9BE_002731"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFN3033430.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="ACG9BE_002732"
     CDS             2591..3376
                     /locus_tag="ACG9BE_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN3033431.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="ACG9BE_002733"
     CDS             3389..4240
                     /locus_tag="ACG9BE_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFN3033432.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="ACG9BE_002734"
     CDS             complement(4364..4816)
                     /locus_tag="ACG9BE_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3033433.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1677 a          813 c         1036 g         1437 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tct
//
LOCUS       DAZAID010000143         4961 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000143 DAZAID010000000
VERSION     DAZAID010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.146"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1338)
                     /locus_tag="ACG9BE_002735"
     CDS             complement(139..1338)
                     /locus_tag="ACG9BE_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN3033434.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..2844)
                     /locus_tag="ACG9BE_002736"
     CDS             complement(1468..2844)
                     /locus_tag="ACG9BE_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3033435.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2844..3500)
                     /locus_tag="ACG9BE_002737"
     CDS             complement(2844..3500)
                     /locus_tag="ACG9BE_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3033436.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3510..4787)
                     /locus_tag="ACG9BE_002738"
     CDS             complement(3510..4787)
                     /locus_tag="ACG9BE_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3033437.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4806..>4961)
                     /locus_tag="ACG9BE_002739"
     CDS             complement(4806..>4961)
                     /locus_tag="ACG9BE_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFN3033438.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1556 a          903 c          608 g         1894 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacgtacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat
     1921 taatgagcca aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa
     1981 tgcagggtaa tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt
     2041 cccaagatct tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc
     2101 ttggtataca gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt
     2161 gattaatgta ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc
     2221 gaatagacct ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc
     2281 aaataaatta gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa
     2341 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
     2401 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
     2461 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
     2521 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
     2581 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
     2641 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
     2701 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa
     2761 agttgacata gataaaataa ctaatatgat aattaatgat tttaaacttt ttctagttac
     2821 ataagcaaat gcatttttta tcattattca acctccaaat tcattttatt tacttttttc
     2881 aactttttac cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt
     2941 gttacaacta ttacacattt atttctatct ttagctaatg tctttaatat attaattatt
     3001 tctccagcag taacactgtc tagattacca gtaggctcat cagctagtat tattggagca
     3061 tctgatacca atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata
     3121 acatttcttt ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct
     3181 gatttattta ctaatctaat attttcaatt ggtgataaat aatctattaa attatagttt
     3241 tgaaatacta aagatatatt attttttcta tgattactat atcctttatt ttgtatatct
     3301 tcattcttaa acaatatttt tcctgtttga ggtttatcaa gtccagcaag taaggaaaga
     3361 agtgtagatt ttcctgttcc tgactttcct actatcgcat aaaacttccc aagttcaaat
     3421 ttttgattta ctcctgacaa aactttttct ttagaatttg cataactata tgctacattc
     3481 tttatttcta atatatccat tattttctcc taacttattt ttgataagat ttctttaggc
     3541 ttcttgatta acattaatga agaagcaaat acaactgata aaacaataat acttattaat
     3601 attaaataac tttgtccaag tgttgttata tttgacataa aactactatt atttattaaa
     3661 cttccaccgg aaatcattga atcctctgag ttaatgaatc catctacaat tacttttagt
     3721 aatacatttc ctaaaaataa ggaagatatt atacttggta tcgatatgaa tattaactcg
     3781 aatataaatt gcattataat ttgtatctta gatgttccaa tagataagaa tatacctatt
     3841 tcataaattc tttcccttaa ccatagaatc aagattaatg aaagaacaac catcccacct
     3901 aacataatag aataagtcat tacttttatt atatatttta ttccactcac tgactctaaa
     3961 gactcttcaa acgcattaga atctttttca acaaaatact ttgactcatc aattttaagc
     4021 tctttcaatt tgtttaaggc taagtctgta gattctgcac taccagaata cattaaaatt
     4081 ttatttgcaa tttcattatt ttctgaatta tttaatatct cttggcttgt tgaataatct
     4141 acaaacacca tattttcact aaaatcagaa gataatcctg tatatgtttc ctgtttttta
     4201 ccagaaaaga tccctataat tttaaattta tgacttttta tttttccact tttttcaata
     4261 tctagtaatt caagatcaac ttcatcacct aattttagat tgttttgttt agcaaattct
     4321 tcatgaacaa taattgaatt cttatcattt tcttctatat ttttaccttc tttaattgta
     4381 aatactccac tactaaataa aatatttctt ttagtattat ttgtagcttc gagtgaaaca
     4441 acattcttaa attcgtcaga taaatcttct ctatttattc tttgttctcc gctaactact
     4501 ttagcatctt ttagttttgc taatccatca tattgaatta ttttttcttc aatttctttt
     4561 aatttttcaa tatctttaaa ttgattaaca ttaaaatatt taccatcttt ttttgttatt
     4621 gatattgaag aattagaact ttcatataaa gccttttcta tttcattgct tgatttcatt
     4681 attgttaaac aagaatacaa gcaagaaaga actatagtta atatgataaa aattattaaa
     4741 gttctatttt tctttcttgt aatatatgct atagcatttt ttatcacttt cctaactact
     4801 cctctctatt ttcttactcc ataactaatt gcatccactg gacaagcatt tttacatcgt
     4861 ccacatctga tacactccag atgattgcaa ttctcaactg aatctttatt catttggcaa
     4921 acttttttac attttccaca attaatacac ttatccttat c
//
LOCUS       DAZAID010000144         4933 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000144 DAZAID010000000
VERSION     DAZAID010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.147"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG9BE_002740"
     CDS             85..972
                     /locus_tag="ACG9BE_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN3033439.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG9BE_002741"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG9BE_002741"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFN3033440.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG9BE_002742"
     CDS             2274..2720
                     /locus_tag="ACG9BE_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN3033441.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACG9BE_002743"
     CDS             2993..4345
                     /locus_tag="ACG9BE_002743"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFN3033442.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACG9BE_002744"
     CDS             4516..4803
                     /locus_tag="ACG9BE_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN3033443.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1696 a          813 c         1029 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gaactgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAZAID010000145         4900 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000145 DAZAID010000000
VERSION     DAZAID010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.148"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG9BE_002745"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG9BE_002745"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFN3033444.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG9BE_002746"
     CDS             complement(1297..2133)
                     /locus_tag="ACG9BE_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3033445.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG9BE_002747"
     CDS             complement(2317..3534)
                     /locus_tag="ACG9BE_002747"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN3033446.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG9BE_002748"
     CDS             complement(3683..3844)
                     /locus_tag="ACG9BE_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033447.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG9BE_002749"
     tRNA            3917..4000
                     /locus_tag="ACG9BE_002749"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4900)
                     /locus_tag="ACG9BE_002750"
                     /pseudo
     CDS             complement(4240..4900)
                     /locus_tag="ACG9BE_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1502 a         1015 c          785 g         1598 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc
//
LOCUS       DAZAID010000146         4781 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000146 DAZAID010000000
VERSION     DAZAID010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.149"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(534..1019)
                     /locus_tag="ACG9BE_002751"
     CDS             complement(534..1019)
                     /locus_tag="ACG9BE_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFN3033448.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(1021..1692)
                     /locus_tag="ACG9BE_002752"
     CDS             complement(1021..1692)
                     /locus_tag="ACG9BE_002752"
                     /inference="COORDINATES: protein motif:HMM:NF037286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033449.1"
                     /translation="MSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTP
                     FTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLS
                     LRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENI
                     VVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLIN
                     SIQED"
     gene            complement(1955..3148)
                     /locus_tag="ACG9BE_002753"
     CDS             complement(1955..3148)
                     /locus_tag="ACG9BE_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3033450.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(3232..3432)
                     /locus_tag="ACG9BE_002754"
     CDS             complement(3232..3432)
                     /locus_tag="ACG9BE_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFN3033451.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3856..4089)
                     /locus_tag="ACG9BE_002755"
     CDS             complement(3856..4089)
                     /locus_tag="ACG9BE_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3033452.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(4096..4527)
                     /locus_tag="ACG9BE_002756"
     CDS             complement(4096..4527)
                     /locus_tag="ACG9BE_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN3033453.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1238 a         1132 c          914 g         1497 t
ORIGIN      
        1 ccaacacaat ttgacgaaga gccactcttt tgggaatcag aggaaagaat caggctttat
       61 agagatattt catctaacaa attactttag actgacagta tatatcagca ggtactgtca
      121 tcgggaacgt agccaatatc gtatccaaaa cagcaggtag agaagctatt tgaggattca
      181 agttattcta tttcaaccat gaagttaagt tcattaaaaa taaagatagt tggtatttca
      241 ataatttttg tatagagtta atcactttat tgatgctaat tcatcttgat tggtattata
      301 atcgtattca agttgcgtgg cgtgcttttg ctagaaaata tataactgtt ctttataaag
      361 aaatataagg attaaacatt ttttatcata gagttttact tatctataga attatgtctg
      421 ttcctaattc agtttgatga attaactgat aaaaaaatat ctactattta aagatacagg
      481 gataagtaag taaaaaaata acatgtcatt agatagtaga cagttccaac tctttattta
      541 ccttttttgt atgctgcact gtcaatccaa gttcctaaat tgttgtatcc atcatcactt
      601 atataatcat attggccatc aataatttcg tcaaaccaca aatattctcc atctggtttt
      661 tgcccaatta ttttaattac tgattgacta gtagttgtca tctggttaaa atcttttatt
      721 ttacctatat taacggccaa aatagcattt cctcgcttat aactctcctg gatttcatat
      781 ttaacatatt ctcgatcaag agtttcttta cctatcagca ccacagtcac tgatgttcca
      841 attaattgat tatctatcca gttttttata gcaatatctc cattacgttt aactttctca
      901 aattcagcct tatctacaaa accagcttcc tttgttcctc gcaaagtcca gctgtttcta
      961 actaccattg ctcttgttac atctgcataa tggaaactaa aaaaaaccct tctcaccata
     1021 ttaatcctcc tgaatactat ttattaaact gacaatttgt tcaacttttt tatctacttc
     1081 tccttcagta tctaattttt ctagcgcttg aaatttactt tttaaggaat catcaaaata
     1141 atcactgaaa ttctcttgaa tcttatcaaa caaaacctta cttgcacctt tggtaaatcc
     1201 aatcggaact actatatttt cattttgaac agataattca aattcttcaa tcattccatc
     1261 tgctgtaact atttcacctg attcaagttt gtttccaaaa acaaaaatac taatcccaga
     1321 ctctgcaata atgttttctc gatatcttct ccactcatga ggcatatcaa ggggaagtgg
     1381 tcttaaagaa agtacgtctt gtatgttatt ttttcttaaa tttcttctct gctttagaac
     1441 tcctgaaaca atactattac caattccaag tccgtaacct gatactattc tataaccgga
     1501 ttcagaaagc ctatatgcta atttctcgac aaattttaat cctttcgatt cagatgtaaa
     1561 tggagtataa tcaacagcac ttcccgaaat aaaaatagtt tttctcaaat acttttttct
     1621 aatacttttc aaaatatcag taatctcaga ataatcatct acttcaacaa cgtttatcga
     1681 aaatctggac aaatcattga tttgtagtct tctttttgtc agttcataat tatatctctc
     1741 ttgatcaaaa aaatcctcag aatcgacaaa attttccttt aaaattatat gggattttag
     1801 attatctttt tagaagttct tgaatgtcgg tatagcccgt gtttccgggc ttttccggca
     1861 tttttgatat tgttagatac ttacaaatgt tcttattacc tctcaccatt tccccctcaa
     1921 aagtagtaaa tggagtagta aaaccggcct tgtgtcaggc tgccagccgt tccatttctg
     1981 cccttgcgga atcgaatgtg gcgtgtgcat aatagttcag cgtcatggtg atattggaat
     2041 gtcccataat gtactgcaag gctttggggt tcattcccgc attggctaac cgggtgcaga
     2101 atgtatgacg catagcatgg ggcgtcgtcg ttttcggcaa cgcttcttca tggctcttgt
     2161 tgtacttctt cacaagcctg ccaaacatcc cgccataatc taccgccgtc atgggatagc
     2221 cgttctgttt gagaaacagg aaattggcat acccgtcaat aatgaacggc tttgcacctt
     2281 tgcggttttt tatcactcgc tggaacgcct gatacacttc ctcattcatg gggatttttc
     2341 tgacgccgct ttgggtcttg ggttcgtcaa tatagtaacc gacgccggta tttctcaaaa
     2401 gctggtggct cacgatgatt acccggtttt caaaatccaa atctttgtcc gtcagtccgc
     2461 acagttccga aatgcgaagc cctgtcccca gcagaataac gattgcgtca taatgctttt
     2521 ggtaaacctt atcaccttga ataaaggaca gaaaactttc ttcctgtgct ggtgtaagag
     2581 gcacctttgg ttccgtatcg tcgtccagaa cgtcactaag ctggaagtca aacgggtttt
     2641 ttcggatgca atcgtcctgt atggcggtgt aaaaagccgc tttcagggaa cgcttgtcgt
     2701 tattgatggt cttataagaa aggccctttt ctttcatccg aagcgcccat tcctttgcgt
     2761 cggacagctt cacactgtca atcgggcagc tccccagccg atcttgttcc agaatacgca
     2821 tcagccgttc ccggcccagt ttggtgttgt gcttcacatt gccccggtgc ctgatctgct
     2881 tggcgtagag ctggcagacg gtcattttct tgccgatggt gtctatcccg tcgtcaaggt
     2941 ctttctggat ttctttctcc ttttcccgga gtgaaatatc ctcccgttta cctgccgggg
     3001 tcttatccgt aggcactaac ttccatgagt aaacaaactg cggttttcca aaggtatcaa
     3061 catatttgta agcatatcgt ccgtcttttc tctggctctc tccagaccgc aaaacgcgat
     3121 ttttgctgtc tcgtcttttt tctgacatct cgtcatgctc ctttccatga cggaaagagc
     3181 cgcatatatg cttataaatt attataacat atctgcggcc ctttttcact attagattgt
     3241 gtccaaagtg tcaattattt tctcaaactg cttgcgtttg atctggatgc ggttgccatt
     3301 cataatgacc cagccagagg ccggattttc ctcggccagc ttacgcaatt tgttttctcc
     3361 gatacggaaa tatttagacg cttcctcaat cgtcagcgtg tatttttccc aaataggcac
     3421 atcatggttc ataatgcagc cccctttcac aaattcaaag gtttgtgtga tttaggggca
     3481 tagacggacg gctgctggca cagctccacg ggactttcac ccccgcgtgg ttctcacgcg
     3541 gccctaccca ttgcctgcga cgcttttaac gctcggactg tggctataag ggagtatcat
     3601 agtcctgcct gtacgtcgtc gcccgcaagc cgccacgctc acattgcggc gtggtgttcc
     3661 tcgctccgct tttcccgtag ggaaaagtat catggcgcac cagccgtccg gctcggcaca
     3721 gacagaatgt gtccgtatgc cctatgatgc gccgccgtta tcttgcgggc atatttatca
     3781 aggtgcgatc aggccaccgg gaaaacaccg aaacggggaa agggtaagcc gtttcatgct
     3841 gccggggccg gttcgtcagt tcgtgacgat agcgtggatg agcttgattt ccagacggcg
     3901 cttcatggcc tcgtccagac acacatgcgg atagccgttt tcgtcataga gcgtccgggt
     3961 acacagcttg ttcatgtaac cgctgtaata ctgcaaaacg cgctctacgg ctccggcatc
     4021 cccagcgcgg gcggcctcga tcaccgaaag gggcaaaagc tcaatgccct tgtagttcgg
     4081 gttcttcatc cgcgtctaac ctcccttcgg catcaacgcc tttagctgtt tccgcagctc
     4141 cgataaggta tttgtcctgt gccgctgaac ggcactccgg gacattccca caaggccgcc
     4201 gatctcacca tctgccatat ccagaacaca gtgcaggatt aaaatactct gctccatgct
     4261 gggcagcgcg gcaaaggctt cggcaacaag ctcattgttg atatgtaggt catagccatg
     4321 cgacgaaaac gtaaagctgt cgctggggta gtgatctact gtacagagct tgtccaaatc
     4381 tgcctgtgac aaagagctta gtgacacttc acggtcacgg cggcgcttca tttcagccaa
     4441 gtagcttttg gcctcgttac gcagtaccgt cttgcaaaaa gcatcgaaca tgcaccgcgc
     4501 ttcatagtcg cgggggatag catccatctt ctcaccccct ttctgtgggg gacttaggtg
     4561 gttctctccc tttccgctaa caagactttg acaaggccgc cagctacccg ctaaatacct
     4621 cctttttgaa aatttctcaa aaaatttttt tggggacagc cgggccgcaa aagacgacaa
     4681 aaatcgcccg gcaggtcgca aacctaccgg gcgagaaaat gctttgctat gctgctgatt
     4741 tacatggtat agtacatact cataaccgga atagccctgt g
//
LOCUS       DAZAID010000147         4701 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000147 DAZAID010000000
VERSION     DAZAID010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.150"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..729)
                     /locus_tag="ACG9BE_002757"
     CDS             complement(<1..729)
                     /locus_tag="ACG9BE_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033454.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGIL"
     gene            complement(937..1863)
                     /gene="glsA"
                     /locus_tag="ACG9BE_002758"
     CDS             complement(937..1863)
                     /gene="glsA"
                     /locus_tag="ACG9BE_002758"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFN3033455.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1886..3313)
                     /locus_tag="ACG9BE_002759"
     CDS             complement(1886..3313)
                     /locus_tag="ACG9BE_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3033456.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(3344..4558)
                     /locus_tag="ACG9BE_002760"
     CDS             complement(3344..4558)
                     /locus_tag="ACG9BE_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFN3033457.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1460 a          998 c          818 g         1425 t
ORIGIN      
        1 tagaattcct ttttgctctt ccaatccttg acagactaat tgaaataacg gcttcgtttc
       61 tgcgcttttc ttatcttctt ctttcagtcc aaggggaaat gcctttcttc cgattgcgat
      121 ctctattcct cgtatgataa aggacttttc atcttccgaa tatcttcttt taaaaaaatg
      181 agtctctaca taatcaatca actcgttaca agcttcttct acatacggat ttttattttc
      241 atagccttcc caattgacat aacgtactaa agctagcaag atgttccgcg agtcaaattc
      301 tgggataatg atttctttat ttttcaaata atcattttct ttaaaaaaat ccaatacgat
      361 ttttttgatt gtatatactt tgctcaaaga aataaattca agatcagcca gttctgtact
      421 cgtgaatata ccattaaaat aataacttaa aaactttaaa aaataagatt gaccgtacag
      481 aaactgtgtg agctcagaaa gagcaatacc atgtttgaat acgagtttat acttatgctt
      541 ctctcttact tgttctttta tacaatattc tagacactcg gctagacttt ctagatcgtg
      601 aaggatcgtt ggtgtagtca cccttagaga aatagctgtt tgttcgatgt ctatttcttg
      661 gaattcaaat aattgttcac acagatacac ttttctaaat atatttcgct cgatgtaatt
      721 ttccagcatt cctgatacac gcctccagtt tttcttatga ttttatttaa tcatatctgt
      781 aaatcgtatt tttgtttcct ttttatttag aaaagccctt tcaaaaacca ataaaaaaag
      841 aaactgggac aaaagtcaaa caagctcaca atttcagcct cattgtaggt agctttgagc
      901 cgtttggact tttaccccag cctctttcaa gaatggttat tcaaagatgt cagcgtctag
      961 ttctttgact acatctttca acaattggat accagctgcg ctgttaccaa atggatctag
     1021 tgccgggctg aagacaccta tgccgtaacg atttggcaca gctgccatca acccgccgcc
     1081 tacaccgctt tttgcaggta caccaacatg gacggaaaac tctcctgatt catcatacaa
     1141 accagcagtt gtcataattg atttgacgat tgtagcactt tcttcagtaa tgatttgttt
     1201 gccattccaa ggagcaattc ctttatttgc cagcacagca gctaattttg ctatgcctgt
     1261 agcggtgaca ttgagggagc attgtctgaa ataagcatcg agtatctccg gtacgtctcc
     1321 ttcgatcatt tgattacctt tcatatagta agcaagagag cgattgatat cacctgttct
     1381 tgattcagaa tgatagattt cttcattcaa tgtgatatta ggatcatcac agatctcttt
     1441 cataaatgac aagattcgct caaatttttc atctgcatct ttaccggcaa tcaacgaggt
     1501 tgttgcaatc gctcctgcat taacaaaagg attcattgga tgtttgcgat gattgatttc
     1561 catattcatg atagaattga aggcaaaacc ggttggttcc gtgttgattg tttgaaagac
     1621 tttttctatt ccgttgtctt tgattgctaa taacaatacg ggaacttttg acatgctttc
     1681 gatggcaaag cgcacatcgg cgtctcctcc ctcaatcagt tgttttgtat ccaaatcata
     1741 aatcgccata cctaattgtt tgggatccac ttcagctaat gctggaatgt aatccgccac
     1801 ttgtcctgtt tgataatagt ggaaattttt tccaatcaat tgttctaatt cttttacttc
     1861 catttgaata accagctttc attttttatt tttctgcatg ctggaattta tgacggcatg
     1921 cgtagatcgt aaatggaata actaagacga taagataaga aacaatcaga atcgtttcat
     1981 aaatcccatg ctgtccagct gcgatactag caggtggaac aaatgagata caaaaagcaa
     2041 aaagtgaagt caaaagacct aaacaagcaa cgattacttt tacaactttt ccgcctggaa
     2101 cctgataggc tctttcaaga ctgtcatctt taaaaaccag cttcaaatag cccgcgaaaa
     2161 acataaagta caacatcagg tagattacaa cagtcaatga catcgctgca agaaatgaaa
     2221 gattattccc cccgccgctt aatgtaaaga cggctgccca gattgttgca acgatcccat
     2281 ttgcgatcat caaatgagca ggaacaccgt gtgtgttttc ttttgcaaaa tattcaggaa
     2341 gtaatccttc ttctgctgct acgtgaagtg cttttgaagg agaggttacc caagaagcaa
     2401 tttctccgat gacacctaaa caaacaagaa tcgcaagaat tttcgcaatc catgcaagat
     2461 ttggatcaat gtgtcttaat aggaattcta acgattgaat cacccctgta ttaagagaca
     2521 aaccatgttg aggtactgta gtagcgacgg tcaatccgcc aaaagtatct aaaatgattg
     2581 ccagaataac caatacaaac attgctaatg gataatcctt tttagggttt tccatttcat
     2641 ttgcatgact ggcagatgtt tctacgccca tatatgctaa aataaaggaa acaaaaacaa
     2701 ccagcgtagg caatttcgaa aaatcaggaa tcaatgtttt catagacagt atcgtttgta
     2761 gtggagcgcc tgaagcgaca taatggatcc ctaagccaaa caagactaat gctggcaaca
     2821 agataccagc tacaaaaccg atccgagcaa tcaatgaagt gtatttggtt ccttttaact
     2881 gtgagagtaa aatagcccag aaaataatca ataggattgc caatttcaga tatttattgt
     2941 tttgtatagc accaataccc gttgcatagg ataatgcacc agttaagaaa tacagcatgg
     3001 tcaagtagcc aactgttatc tcaaaccatt ggaaaaagat tgcggcaaaa ccgaatcgtt
     3061 ttcccaatgt gcgactgacc caagtataga caccgccttt ttcccatcct ttcaccgttg
     3121 ccatctctgc tgcgcacaat gccacaggga taaaccatag caaaccgcct aataacaaga
     3181 aaaatactaa gctgaagcct gaggtcgcga atgtgggata ttcatataat gaaacgacca
     3241 ttgagatggt catagccaaa aagccaaaga acgttaattt ttttgcagga attttcgttt
     3301 tgctttgttg catgaatctt cctcttttct atttgaattt gtttcaattg agtttgttgc
     3361 caaagaagaa aaagtccaat ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa
     3421 agtgcatcgc cgattttaaa atcactgttg tcagcacata ggcatacacg gcagtaatga
     3481 tgacaccaat cacttgaatc cctaagaagt accagctgct tggattcgtt agtaaatggc
     3541 tgttagatgc aaagatccct atcagaatcg tacctgtgaa tccgcccatg ccatgaacac
     3601 cccacacatc aagtgcatca tcccattgtt tctttttacg aaaatcaaca gcagcatgac
     3661 agacgatacc cgcaatgatc ccaatcagca tggcactttt tgcttctaca taaccggcac
     3721 aaggcgtaat ggctgccaat ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc
     3781 catttccttt tacttttgca atgatcagcc aagtaatcat cgcaaatgca agagcgatga
     3841 atgttgaacc aaatgcattg actgcttgtt catctgctcg aagtgcacca ccggaattaa
     3901 aaccgaacca gccaaaccaa agcagtcctg taccgatagc agctgccata agactgctat
     3961 gctgacttct ttctcctaaa ggaatctttc tttgaccaag tgtgaggata catgctaaag
     4021 agccgaatcc agctgtcgtg tggatgactg ctccaccagc gaaatctcta aatcccatct
     4081 gatccaaaaa tccgccaccc catatccagt ggcaaacagg gaaataaatc agcagattcc
     4141 aaaaaataac aagtataata tagcctttta aattcaatct ccctgcaaaa gctcctgtca
     4201 tcaaaggaac ggtaatcacg ataaacatca gttggtaaag aaagaacatt aaaaatggaa
     4261 tcgttgcacc atgaaacatg ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg
     4321 ccgggtttcc aataacaccg ccgatatccc gtccaaaggc caatccgaat ccaccaaata
     4381 cccacataat cgttgttatc ccaacggaga gaaaagattg agacatgatc gttagtatat
     4441 tattcttccg tgctaatccg ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca
     4501 ttgctgtaca taaaatcatg aatgctgtat ctcctgtatt gataggtaat gtattcattt
     4561 ttttcgcctc ctttctacga ttacgagtat aaaagaaggt gtagttgttt ttttcttctg
     4621 tttttttgaa ttaatacttg catttataaa agacagacaa ttaataaaag cttgatgtgt
     4681 tgtgctcttt ttcgtttttc a
//
LOCUS       DAZAID010000148         4590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000148 DAZAID010000000
VERSION     DAZAID010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.151"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACG9BE_002761"
     CDS             186..2855
                     /locus_tag="ACG9BE_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFN3033458.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLAPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACG9BE_002762"
     CDS             complement(2946..3560)
                     /locus_tag="ACG9BE_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3033459.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="ACG9BE_002763"
     CDS             3680..4219
                     /locus_tag="ACG9BE_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033460.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="ACG9BE_002764"
     CDS             4216..4554
                     /locus_tag="ACG9BE_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFN3033461.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1420 a          791 c          859 g         1520 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggctcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggagaaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgacatt
//
LOCUS       DAZAID010000149         4583 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000149 DAZAID010000000
VERSION     DAZAID010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.152"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..482)
                     /locus_tag="ACG9BE_002765"
     CDS             complement(12..482)
                     /locus_tag="ACG9BE_002765"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFN3033462.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="ACG9BE_002766"
     CDS             complement(641..1099)
                     /locus_tag="ACG9BE_002766"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFN3033463.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="ACG9BE_002767"
     CDS             complement(1270..2415)
                     /locus_tag="ACG9BE_002767"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFN3033464.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="ACG9BE_002768"
     CDS             complement(2428..3345)
                     /locus_tag="ACG9BE_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFN3033465.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="ACG9BE_002769"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="ACG9BE_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFN3033466.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1431 a          840 c          868 g         1444 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaa
//
LOCUS       DAZAID010000150         4569 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000150 DAZAID010000000
VERSION     DAZAID010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.154"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..2061
                     /locus_tag="ACG9BE_002770"
     CDS             121..2061
                     /locus_tag="ACG9BE_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFN3033467.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2148..4070
                     /locus_tag="ACG9BE_002771"
     CDS             2148..4070
                     /locus_tag="ACG9BE_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN3033468.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(4350..>4569)
                     /locus_tag="ACG9BE_002772"
     CDS             complement(4350..>4569)
                     /locus_tag="ACG9BE_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFN3033469.1"
                     /translation="DYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAVGSE
                     EEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         1512 a          774 c          939 g         1344 t
ORIGIN      
        1 atcaattttt ttagcgaaat ttctatttat tccaagaatt tcagttatta cggaatcatt
       61 ttgtgcttaa taaaaagaaa ccgtttttat atactataaa tgtagaaagg cggtaggaaa
      121 atgaaaaaaa ttattgcaat cacatcctgt ccagttggta ttgcccatac atacatggca
      181 gcagaaaact tagagaaagc ggggaaagca gttggcgccg aggtaaaagt cgaaacacat
      241 ggttccatcg gtattgaaaa tgaattgact gcaagagata tcgaagaagc agctggtgta
      301 atcatcgcag cagatactaa aatagataaa tcacgattcg gcggtaaacc tttgataacc
      361 gtaggtgtgc aagaaggaat ccaccatgca gatgagttgg tgagagatat tttagcagga
      421 aaagcgccag tctataaaag tacggaggca attatttctt ccgaaaataa aagtgaatca
      481 gaaaatttag gtaaaaaaat ctataagagt ttgatgaatg gcgtttcgta catggtacct
      541 ttcgtagtta caggagggtt attgatcgcg atttctttaa cattaggagg aacagctaca
      601 cctgaaggga tcaagatacc agaaggaacg atttgggcaa cgatgaatag tatcggaagt
      661 atcgctatgg ggttgatggt tccaattcta tctgcattta ttgcgcaaag tattgcagat
      721 cgtccaggac ttgttcctgg atttgttggt gggatgttag cggcaaacgg agcattatat
      781 ggtagtgatg cgaatgcggg attcttaggc gggatcatca ctggattttt agcaggattc
      841 attgctttag gaatcaagaa agtgcgagta ccgaaagctt tacaatcgat catgccaatt
      901 attgttatcc caattttagg atcattggca gttggttttg tgtttatcta tgttattgga
      961 gaaccagtag ctcttctatt tagttcattg actcactttt tagcagggat gcaaggagga
     1021 agtgaaattg ttctagctat gatcttagga gcgatgattg cttttgatat gggggggccg
     1081 ttcaataaag tagccttttt gttcggatct ggcttgattg cagaaggcaa ttattccatt
     1141 atggggccaa ttgctgtagc tatctgtatc cctccattag cattggggct ggcatcattg
     1201 attttaaaaa ccaaatttac gaaaacagaa cgagaagctg gtaaggcgtc cttagcaatg
     1261 ggactgtttg ggattacaga aggcgctatt ccgtttgctt caacagatcc actacgggtg
     1321 attccaagca ttgtaatcgg ggcaatgaca ggaagtgtga tcgcgatgct ttcaggagtg
     1381 acagatcatg tagctcatgg cggtccgatc gtagcggttt taggagcagt ggatcacgta
     1441 gccatgtttt ttgtagcagt gattgctggt attgctgtaa ccgtgctaat gttaagaatc
     1501 cttaaaccga cattacaaca aactgcggtt gctgcagcaa atggtgtgac agtagcagat
     1561 ctatcagaga ctgaaacaag tgatgtttat acgaaaactg aaacttttga ttctatcacg
     1621 gatattatgt cagatgaagc aatcgtttta ggtgaaacag caacaacaaa agacggagta
     1681 atcgaagatt tagtccaacg tttagaaaaa acagcggcga tcacagataa aaccagattt
     1741 aaagaagtca tctatgatag agaacatgaa agtacaacgg gaatcggtat ggggattgct
     1801 attccacatg gaaaatcagc ttatgttctt aaaccaacag tggcatttgc cagaagtcaa
     1861 tctggggtag attggcattc tttagatgga aaacctgcac atatgatctt tatgattgct
     1921 gtacctgaaa acagccaagg tgatatgcac ttgaaaatac tgcaacgtct atcacgtaaa
     1981 ttgatggatg atgacttccg tcaagcactg atggaagcgc cagataaaac aacagttcac
     2041 caattattaa gcgaaatgta acataagatg aaaaatcgac cgtgatgaga cataatagat
     2101 gtgttatcat gatcgatttt ttatttagaa gggagtttct gcacattatg aatcagcgtc
     2161 agcaatcttt gatctatcaa ttaagtgaga acaataatta tataacaaca aaaaagctag
     2221 cagagcattt ccatgtgtca gaaagaacga ttcatagcga tttgaatacc attgaaactt
     2281 ggttgagaga caaacatctg cctttaatgt ttgaaagaaa aaaaggtaca ggtatcttgt
     2341 tgaatggtct gcctcacgaa agggaacaac tgaaacgagc gcttaatgaa gagactcaaa
     2401 cagaaatgga taaaaaacag cttcgtcagt tattgatttt ccatctgcta gtagcaaaag
     2461 acggatttag tcttgaagaa ctttcagaaa aactatatgt tggaaaacgt acgattcgta
     2521 aagaattgac agagttgcaa agcttttttg agtatcacca tttacagcta gtatccaaaa
     2581 caaaactagg aacttttctt aaaggcgatg aacaagaaaa aagacagttg cttgttaaga
     2641 cattacgaaa tatgcagaaa gaagatccac aaagtccgtc gttgaaagaa ttcttcgcta
     2701 aagatacatt aaaagttatc caacatacat tacaagatgt tttttatgaa aaccatttag
     2761 aacagccgaa tggactggct agtgtggaga ttcacatcta ctttatgctc gagcgaatga
     2821 aacagtacca gaaagtgaaa ttaagtaaag acgaaaacga agtcgtggaa cacacccaag
     2881 cacaaaagtt aagtagtcaa atattagcaa aactagccac catctatcca attgaatttt
     2941 ctcctgatga aatcaattat ctggctttgc gtatcgccaa tctttttaca aattcacaag
     3001 cagccacacg ttttcaaaag gaatctagcg cacttgctga tcatttgatc gaacaagtgg
     3061 aacagtttct aggctattca ttaaaagaag atcagctact aaaacaaaat ttaaggtctc
     3121 atttatcttc aacttatttt cgactccatt atggtttgca gatttctaat ccattgacga
     3181 aaaatgtgtt tagtacgtac acacaacttt tcttagtttt acagttaatt ttagaagatt
     3241 attttgaaaa ggaaaatttt tatgttccac aagaggagac agcttattta acgatccatt
     3301 tccaagcagc gatcgagcga cacaaacatc agaaaagtag aagatatcaa acggttttag
     3361 ttagtgaata ttcaaaagct atggctactt ttctcgaagc acggctgaac agagaacttc
     3421 cagaattaac gattatggat ctagttgaat ataagccaga attaaagaga caagattttc
     3481 cctctgttga ttttatcttg gctacgttac catttaaaca tataagcatt ccagtaatcg
     3541 agatctcacc aatgatcaca gaaactgatc tagcctattt gacaaaatat atgctggagc
     3601 atgtgcctat caagaagaaa aaaacatttg atctagcaag ctttactcat ccatttctga
     3661 tctttcctca acttgaacgg acagacccag ccgatatttt aaactttatg ggaaacgtct
     3721 tagtggaaca tcattatgtg gagccggaat tcatcgattc tgtcttggaa agagacagtc
     3781 actccagtac tagagtagca ccatttgtaa caatccctca cggtaatccg ctgtatgtga
     3841 agcattcgat gatttctatt gctacaatga aagaaccagt cctatggcac ggggaacaga
     3901 tacgtatcgt actgctatta gcaataaaga aaaatgattt gaaacatgca gagttcaaaa
     3961 aaatattttc tgttattcac tatctagaaa agcagccaga acaaatggct cagttgatgc
     4021 aatccaatca tccgctagaa atcttaacgt tgttatctag ttatgaataa gtagcgatta
     4081 aaaaactgcc tttatattta taataattct atctatttat ttggaagtag aaaactaaat
     4141 acaaaaaaag aggatgtggc caaagttgtg gcacatcctt ttattttgag tttcttataa
     4201 ttttttatag ttgcttacga tgtcttgact actggtaaac ggtgttcaag agacaatttg
     4261 tcggcaataa ggcacaattt tgtaaaatat ggaaagctat ttttttaaca ttgtttctta
     4321 gtgcttgaaa aatcattttg aatacttctt tatacgatcg atgtacgttg atttaagtct
     4381 aaaacttttc ttttgattcc ttctctgact tttctgaatt ccgttaatat ttcttcttcg
     4441 ctcccgacag cttgtgcagg atcttttagg tcccagtgaa ggtgttcgtg attgtttgga
     4501 acggcagggc acttgtcttt ggcatcacca caaagtgtaa tgatcaagtc tgcctgagta
     4561 aagtaatct
//
LOCUS       DAZAID010000151         4537 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000151 DAZAID010000000
VERSION     DAZAID010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.155"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..1014)
                     /gene="murB"
                     /locus_tag="ACG9BE_002773"
     CDS             complement(79..1014)
                     /gene="murB"
                     /locus_tag="ACG9BE_002773"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFN3033470.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1087..2007)
                     /locus_tag="ACG9BE_002774"
     CDS             complement(1087..2007)
                     /locus_tag="ACG9BE_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN3033471.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2120..2872
                     /locus_tag="ACG9BE_002775"
     CDS             2120..2872
                     /locus_tag="ACG9BE_002775"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFN3033472.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3736..4269
                     /locus_tag="ACG9BE_002776"
     CDS             3736..4269
                     /locus_tag="ACG9BE_002776"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFN3033473.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1339 a          894 c          805 g         1499 t
ORIGIN      
        1 catttgcgta aacaaccaca aaaaagagca ggacggccac cgtcctgctc ttttacttat
       61 tttaagttcc attacttatt aattggctaa acttctgctt gtttctgata ttgcttgttt
      121 ttctccaatg atccgcactt ctgtttctaa agacacatca aatttttctt tgattacttc
      181 ttggatgtgc gcaatcaatt caatataatc tgtcgctgtc gcatgatcga tattgacgat
      241 gaatccagca tgtttttcag agacttgtgc accgccccat ttcaggcctt gcaatcctgc
      301 ttgttggatc agctgacctg tatagtgtcc tactgggcgc ttaaagacac ttccgcatga
      361 agggtattct aatggctgtt ttgattgacg caattcggtc aactcatcca ttcttgcttt
      421 gattgcttca taatcccctg tttgcaaact gaatcgagct tccaatacga tagcacgtaa
      481 ttcttggact ttactgtgac gataagaaaa taccatttcg tcttttgtca aagttttcaa
      541 tgttccatct tcaagcaaaa gatccacttc agcaaaaaca tctttgatct ctccgccata
      601 cgcacctgca ttcatataaa ccgctccacc gacacttccc gggatgccgc aagcaaattc
      661 aaatccagtc aatgattctg ccaaagctgc ataagtcgta tcaatcagtg aggcacctgc
      721 ttccgcaatg accatcgttc ctttgacatg aatctgtttc atttcagtca gcatgatgac
      781 tacaccgcga atcccgccat cttgaacgat cagattgctt gcatttccta ataccatcca
      841 aggtatatca tggattcggc aataatcgat gatttgcttt acttcttctt gttttttcgg
      901 gaatgcaagg acatcagctg gtccacctgt tttcgtaaac gtatagttca tcaatggttc
      961 atcaaacaat atagtaatcg tggctaagct ttcaactatt tcttctttat tcactgagga
     1021 ttttcctttc ttttctcaaa cgtcctcatt attctatcac gcttaataca atcttgccag
     1081 tcttgttcaa agttgatgac ggtagtgatc tttaagcact tcctcacata attcatgact
     1141 tcttacaact tctttttgtt taagcaattc tgtttccgat ttacctaaac ggatctgaga
     1201 aaagaaggcc tctaccattg gctcaaaccc gcgtttgact agcgtgtttg tccagtcgcc
     1261 aaattctttc actttttttc catcagcatc ttgaatgaca agctgggtca gatcgtttac
     1321 ttcataagta ccatttttac ttgttacttg atatttttct atattagcgc cacttgctaa
     1381 atccatcgtt aaaatagccg tttgattttg tgtttctaat tgtaaaatgg catattccaa
     1441 aaattccttt gtttctctta tctggtattt tgttctcaaa atgggttcat ctaataaata
     1501 cacagctgta tcaataagat gtaaaaacaa atcataaagg acgaactctg tcgtttcttt
     1561 tgccgctatt cgattttttt gcaggtggat caaccgctta tctgggatac gtttcaattc
     1621 ttctaccata ggagcaaagc gccggttgaa accgatcatc aaaattattt ggttcttatc
     1681 agctaattct tgcaattcac gaacttcatt tagatggaca ctcagtggtt tgtcaatata
     1741 aacatggata ccattttcta gacatttttt ggctaattca taatgtactt ttgttgctgc
     1801 gtggatcata catgcttcga ttttttcttc taaaagctca tcgatcgttt catatagatg
     1861 ttggaacccg tactcttttt gaattctttc ttttgtttct ttatttcttg ttgcaaaata
     1921 aaaatcaagt gttccttgtt ttttcgcata agttggtaaa tatgcttttt ctgcgatatt
     1981 ccctacaccg attacaccaa tcttcatcta ctttcctcct ggatcattta gttactttca
     2041 tcttacacca tttttttcta aaatgaggta caatagtatc gcaaaggtaa ggaaattaat
     2101 tcgaaggagt gtgcttttat tgaaatttat ctcttggaac gtaaacgggc ttcgtgccat
     2161 cgtaaataaa aattttttag aagtttttca tgagttagac gccgattttt tctgtctgca
     2221 ggaaacgaaa ctccaagcag gacaaattga tttagatctt ccaggttatt atcaatattg
     2281 gaattacgcg gaacgaaaag gctattccgg cacagcgatt tttgctaaaa aaccagcatt
     2341 gaatgccact tacggaatgg gtatcgatat ccatgataca gaaggccgtc tgattacttt
     2401 ggaatattcc gatttctttc ttgtaacctg ttacacgcca aattctcaaa gcgaattaaa
     2461 acgtttagat taccgattag aatgggaaga agccttttat aattatttgg aaaatctaaa
     2521 aaaacaaaaa ccagtcatcg tgtgtgggga tctaaatgtc gctcatcaaa agattgattt
     2581 gaaaaactgg aaaacgaatc aaaaaaatgc tggattcact ccagaagaaa gagcggctct
     2641 ttctcgactg ttggataacg ggtttatcga tacattccgt tatttttatc caacccagga
     2701 aggtgtctac tcttggtgga actaccgatt caattcaagg aaaaacaatg ctggttggcg
     2761 gatcgattac tttttgacga gcaaagattt agagccagga ctagctgatg caaagatcca
     2821 tacggacatt atgggcagtg atcattgtcc agtagaatta gatttgaaat aaagaggttg
     2881 tgaaaaagtc gtttagtttc gagtattaag aaaaattttg tgaaaatggc tttccacatt
     2941 ttgcataaat ttggcataat accgagaaac tggcttttga acactgttta tcgagagatt
     3001 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat
     3061 ttttcgtttg gttgggctta ttaccgcaga tacaagcttt tgaacaccgt ttattaagag
     3121 gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     3181 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttattaa
     3241 gaggttgtga aaaagctgca ctgcatcaag taataagaac agccaaacaa aaatagttcc
     3301 tcatattttt cgtttggttg gacttattac cgcagatgca agcttttgaa caccgtttat
     3361 tgagaagttg tgaaatcagc gtcctgacct gagtattaga gaaacaaatg gcaaaatagt
     3421 tcctcatatt ttgctatttt ttgagctaat accgagggtc agctgattga acaccgttta
     3481 gtggagaagt tgtgaaaaag tcgcctactt tcgagtcgta agaaattttt ttgaaaatgg
     3541 ttcctcacaa ctttgcataa atttgactta ataccgagaa gtcatgatcc ccatctaact
     3601 ccaaatcaca aggaatgagt tcaaaaacta gcttttctca acaaatatta cataaaaaat
     3661 tctatgtttt ttgttaaatt tatttgcttt tttcaaaagt atgttacagt aatttcccaa
     3721 ggaggtttta cagaaatgaa ttttgttgcc atggattttg aaactgccaa tcatcagcct
     3781 tatagtgctt gttctcttgc gctagtcatg gtaaaaaata gccagatcgt tgatgaattc
     3841 tatacattga tacagcctga gactcctttt ttctggcgaa atgtccaaat tcacggaatc
     3901 catcaagaag acgtacgcaa tgcgccgaaa ttcccagaag tctggcaaac tatccaacca
     3961 tattttcaaa aaaatcgttt agtcgttgca cataatgctg cttttgatac aaaggtctta
     4021 gcgggatgct tagattatta tcatcttgaa cagcctaatt atctttcttt atgtacagtg
     4081 aaaagtagcc gtcgcctgtt tcctgaattt ccaaatcatc gattgaacac tgtgtgcgaa
     4141 aatttgaaca tccaattaga tcatcaccat gatgcattgg aagatagtcg tgcatgtgcg
     4201 gaaatcctgc tttatcaaga aaaaaaattc gggacagatc cattgaaaaa acttgtgact
     4261 ataaagtaaa agaatactta tgaaacacac aaagaggtcg tgagcctgcc gtttcactcc
     4321 gagtcacaag gagcaatttc aaaaaatagc ttctcatgtt tattaaatat tgtgactggt
     4381 gaccgaggag ctggcaggag aacaccgttt attggagagg ttgtgaaaaa gctgttctgc
     4441 atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcatt tggttggact
     4501 tattaccgca gatgcaagct tttggacacc gtttacc
//
LOCUS       DAZAID010000152         4521 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000152 DAZAID010000000
VERSION     DAZAID010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.156"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            238..1539
                     /locus_tag="ACG9BE_002777"
     CDS             238..1539
                     /locus_tag="ACG9BE_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN3033474.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1566..1826
                     /locus_tag="ACG9BE_002778"
     CDS             1566..1826
                     /locus_tag="ACG9BE_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFN3033475.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2825..4060)
                     /locus_tag="ACG9BE_002779"
     CDS             complement(2825..4060)
                     /locus_tag="ACG9BE_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN3033476.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1329 a          706 c          912 g         1574 t
ORIGIN      
        1 tttttgaaat gatatgtttc aatcactaga tttaatcatt ttgatttttg ttaattatcg
       61 aatttttttg ttgctcctat gatttagaaa acttattttc aggattctta aaaataaatt
      121 tgattattta agagaatata ttgaaataaa atttcttaat tatataatta gttatgaaca
      181 ttctttgcgt tgtcatgcaa tgaatggttc atattttatt gaaaaggagt ggttttagtg
      241 gaaaagctga tgaaatgggt cgaagagaaa cttgtcccac caatggcaaa aattggtaca
      301 cagcgtcatt tattagcaat tcgtaatggg gttgtttcaa cattgtcatt gattttgatt
      361 gggacatttt ttatggtctt tatcaattta ccgtttcctg gatggaatga atttattgcc
      421 ccttattcgg caacgattgt tttacccttt aggatgacga tgggattgat ggctatttat
      481 gcaacttttg ttatgggttc ggatctagct aaatcatatg gattagatag tgtgacaggt
      541 ggtatattat ctttaggaac gttctttatg ttacaagttc ctgtaaacgt tttaactcct
      601 gaagaggcac ctcttggctg ggtcttacct atgagttctt tgggagcatc tgggatgttt
      661 gcggggatct tatctatgat ttttgcagtg gaagtatatc gtttctttaa aaatcgaaat
      721 attactatca aaatgccaga acaagtgcca cctgctgttg cccgtagttt tgaggcattg
      781 attccaggtg cagtcatttt aacaattact tggctgattc gaagcgtcgc gggctttgat
      841 gtaaacgctg ccttattatc gttattcgag cctttaacca atattttagg gaataatttg
      901 cttggtgttt tactaccaat gtttttgatc catttgttgt ggtcatttgg tatccatggc
      961 atgagtattg taggggctgt tgttcgtcca atgtggctga tcatgttgga tgagaatgca
     1021 aaggcattgg cagatggtac agctgccacc caattaccct atatcgctcc tgaacagttt
     1081 tatcaatgga cggtaacaat tggtggtgca ggagcaacga tcgtggtatc tgttttgttt
     1141 atattcttct gtaaatcaaa attcttgaaa gaggttggtc gtttttctat tattcctggt
     1201 atcttcaata tcaatgagcc cataattttt ggagctccta tgatgttgaa tccttacatg
     1261 ttcatcccgt tcaacttagt tcctttagtt ttgactatta tcagttattt tgcagtcaaa
     1321 ttagaaatgg tcaatagatt tactgttttg ccaacatgga cattaccagc tccaatcggc
     1381 ggttatcttt cagcaggaaa tgactggcgt gttgttatct tgatcatgat caacacattg
     1441 atagcattta ttatctatta tccatttgta aaagcttatg ataaaaagat gctgtcagat
     1501 gaacaaagtc gtggagaaaa cgaagtccag actgtctaag tttggttttt agaaggtggg
     1561 aaacaatgta ttggctgatt ttattttttg tttttatctt tttattaacg gctagtcacc
     1621 ttatattgaa tatgctagct acatatcata ttcaaatcaa tcgatggatt tgggcactgg
     1681 catcattttt gatcgtgatt cttccaaaga tcattgtacc gcatatgaat gttttgtttt
     1741 catgggggac atatgtactt tgtggaattt ttgctatcaa ttttatgatc gagcagcata
     1801 gatggtttgt cacatcgaag ctttaaatgt tctaaaagtg gttgtaacag aagtggttag
     1861 cttcaagaaa taagaagaaa tttacgaaaa ttgcttctta aatttttgtg aaatgttggc
     1921 ttatttttcg tttggttgaa cttattaccg cagatgcaag cttttgacca ccgtttattc
     1981 ggaatatggg actgtgacaa ggcttttgtc acagcccttt tttacataaa gattattatt
     2041 gttttaactg ttttggcaga aagaaggagg aaaaaatgga aattttatca gcaatggatt
     2101 tagaattagt agatctacta ccaaagtgat caagtcaaat atagatttgg gagcttacaa
     2161 ccatatagta ggggctgcag tatggtgtat gaatggtaat atgtatgatg tgagtgttta
     2221 ttcaaatcat gaaacgtgta tagaaataat tgtgctggga atagcaatca gtcatggtga
     2281 acgtgacttt tagtgtatcg ttgctgacga catagaacat tctgacatga tctactcaat
     2341 atgtggggat tgctggcagt ttatatagga gtatgcatcg gattgcgaga tcattgtctc
     2401 aacagaagag ggaggttgat tatcaaagat aaagtggtaa atcctacatt tgatttagta
     2461 attgttgagg ggactagcgg cacaaatctc tacaaagcga tgcttctaga tctatatgac
     2521 acattttaag aaaatgtatt tgcctaatta ttatggtctt tcttttgaag aaacgttgag
     2581 atgatccaac cagagaaagg tgtcaaagaa tttgactaag aataaatggc agacgctacc
     2641 tatgctcatt tggatagcgt cagatctttt attttaagga tgattttgtg ttttttagac
     2701 accgtaattt aagtaactct aacttttcaa tccactattt actttttttg tattttcact
     2761 gtctgggtct cgaatagcga aattactgat gagtctctat taaaaaagct ttttctactt
     2821 gaaactatat tgggtggttg ctcaggaagg tttcgatttc ttgaattgct tgttgtggac
     2881 catttattgg aaaaacggct tgcagcgtgc gtgcctgctg acgaatcttg ggtgaagaaa
     2941 cttcgcgcag agcaatctgc agttttcttt cagtcactgg ttttgtgagg gcgattgcgt
     3001 ttccgtattt tacacaatag tcaatattac agctttgctc aaattgcatc cccacaccga
     3061 taaacggttt gccggattca caggcagttt ggacggtccc ttccccgccg tggataaaag
     3121 aaaaatcaat gaattcatgc aaaagatgac tagacagata ttctgttatt aaaagatctt
     3181 ctgagtcaga ttgaatgtca tcaagcagat agtcaatagg tgcaataacc gctagatccg
     3241 gttgtttttg taagaagaac aaaattttgc gtaacatacg cggattgcct gaactaccca
     3301 ttgcgaaata gataatacgt tttctttctt gttgctttcg ctgtataaaa gttagcactt
     3361 cttcaggcaa ggaagtactg agcttagcaa aaattggacc aacgattcga taattttccg
     3421 gcagctgttc ttcttccaaa aatagtttag gcgttgtgat gagatttaga tcagcatcca
     3481 tcatttgtaa acttgttttt ggtaaaggaa gcatttcttt ttgtgcagcg tgtttgaagg
     3541 cattggggat ccatcgggta tttactatac gcttttttgt ccagtgccaa aggggattgc
     3601 ttaaagggcc gaagaaacgt aactcgtggg gcatcagcgc atgctctgat tgaaaaaaag
     3661 gttcactcaa agcatagggt ttaacatata ctaaaggaat ctttttattg cgggccgata
     3721 aaaaaattgt cacattcgaa cctgtcacaa tcagtcgagg ctcaaactta gcaatcagac
     3781 tctgttcact agtgatacga gaggcaacca tttctttggt aaaagggttt ttaagtgtac
     3841 gcaactggtc aaattttatg atttgttctt cttgtttttt cgtaaggatc ggcgctagta
     3901 aatgatactc aaagccactt tctgtgatca aaggagcaaa agttttagaa aagccaaaga
     3961 atacacagta ataacggtct ttcatagctt tggcaacctc aatcattcgt gttgtctcag
     4021 ctaaattgaa tactgcagga gcaaaaagaa ttgtcttcat gaaatcctcc ttattggaat
     4081 gagtctatcc cttcgtttct tactagtata ctaaaaaaca cagcgttcga gtagcctatg
     4141 tatattttga atattaaact gtctctcgct gttttcacaa cttctaaatt taaattttgt
     4201 aatcaagttg aaactccaat tttcaaaaaa acacgcaaca attttttgat cgatttatat
     4261 gttgtgccag ataaatacga gaagttgtgt cattaaaaat aaacatgaga acataaaaaa
     4321 ataaaataat tatattaatt gataggtgtg ctgtcgtcat tatacacacg taagaaagaa
     4381 atagttatgt ttaaaaatat tcttagactt tctcgccatg acaattttca ctatgtgtta
     4441 taactgtaaa cgataattat tcagataaca caaaaagaaa tcaaggagca gatacgccag
     4501 ttgtttaatt tgaatattat t
//
LOCUS       DAZAID010000153         4433 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000153 DAZAID010000000
VERSION     DAZAID010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.157"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACG9BE_002780"
     CDS             complement(543..2048)
                     /locus_tag="ACG9BE_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN3033477.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACG9BE_002781"
     CDS             complement(2045..2731)
                     /locus_tag="ACG9BE_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3033478.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG9BE_002782"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG9BE_002782"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFN3033479.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1238 a          919 c          730 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa agggatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAZAID010000154         4365 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000154 DAZAID010000000
VERSION     DAZAID010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.158"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..1075
                     /locus_tag="ACG9BE_002783"
     CDS             152..1075
                     /locus_tag="ACG9BE_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFN3033480.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1081..2334
                     /locus_tag="ACG9BE_002784"
     CDS             1081..2334
                     /locus_tag="ACG9BE_002784"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFN3033481.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2468..3814
                     /gene="gor"
                     /locus_tag="ACG9BE_002785"
     CDS             2468..3814
                     /gene="gor"
                     /locus_tag="ACG9BE_002785"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFN3033482.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1504 a          690 c          939 g         1232 t
ORIGIN      
        1 tcacaatctc tttttatatt tggacagaat atatcgaact ttattttgat tttttgtgaa
       61 ataattttca aaaatccgac tttaaataga agaagtattg gaaagttttt agagctatgc
      121 tactattatg aagttgtagg aggagaaaat tatgatcgaa ctcataacca cagtagaaac
      181 tattgaacaa gcagaacagt tgcttgctgc aggtgcagat acactatatt tcggtgaaga
      241 gcaatttgga ttgcgtctgc ctgcttcatt tacacgagaa gaacagcgac aattggtaga
      301 attagcacac aagtatggta aaaaagcgaa tattgctgta aatgggatca ttcatcctga
      361 aaaaatgaaa aatgtacctg aatatttggc ttttttaaaa gaaataaacg tagatgtgat
      421 cacagttggt gatacaggta ttatttatac gatgaaaaaa aatcctgaat tgcgtatacc
      481 gtatatttat gatgctgaaa cattagtaac gagcgcacgt caaatcaatt tctgggcgaa
      541 aaaaggcgca gttggagcag tacttgctcg tgaagttcct tttgaagaga tgaaggcgat
      601 ggaagaaaaa ctcgatattc ctgtagaaac ccttgtgtat ggtgctacat gcatccatca
      661 atccaaacgt ccactgctac agaattacta taactatacg aaacaagacg aacaaaaaga
      721 tcgtgaaaga ggtcttttta tctcggaacc taaaaaagaa gagacacatt attctattta
      781 tgaagatagt catggcacac atatttttgc cagcaatgat ctgaatctct cgaatgaatt
      841 gaatgaactt tatcagcatc attactgtac atggaaattg gacggattat ttactccagg
      901 cgaaaaattc gtaaacatca ctcgactatt cgccgaagca aaaaaacaaa ttttagaagg
      961 tagctgggat caagaaaaag cggaaaaaat gactagcgaa atcagtaaat tgcatcctga
     1021 aaaccgtgaa ttagatacag gattctttta tattgatcct tctgctatca aataaggagt
     1081 atgatgaaag aaatgacagt aaaaagaaaa ttgaaacgac cagaaatcct agcgccagca
     1141 ggaacactgg aaaagttaaa aaccgcgatc cattatggag cagatgcagt atatattggc
     1201 gggaatgcat atggtttaag aagccgtgca ggtaatttta cttatgaaga aatggctgaa
     1261 ggagttgctt ttgcaaaaga acatgatgcg aaggtatacg ttgctgctaa tatggtcacc
     1321 catgaaggaa atgaagatgg agcaggggaa tttttccgca gtttgcgtga tgtcggtatt
     1381 tctgcagtga ttgtttctga tccagctttg atcgaaattt gtgcgacaga agccccaggt
     1441 ctgccgatcc atttatccac tcaagcttca gcaactaatt ttgagacatt ggaattctgg
     1501 aaagaagaag ggttggaacg tgtcgtttta gctcgtgaag tttcaatgga ggaagtggca
     1561 gagattcgta aaaatacgga taccgaaatc gaagcattta tccatggtgc catgtgtatc
     1621 tcttattctg gtcgatgcac attgtccaac catatgtcga tgcgggatgc aaaccgtggc
     1681 ggatgttctc aatcttgtcg ctggaagtat ggattgttcg atatgccttt tggtcaagaa
     1741 cgaaacagtt taactgatac aggcgaagtg gaagaagaat tctcgatgag tgcagtggat
     1801 atgtcaatga tcgaacatat tccagaattg attgaaaacg gcgtagacag cttcaagatc
     1861 gaaggaagaa tgaaatcgat ccattatgta tctacggtgt caaatgtcta taaagcggct
     1921 gtagacagtt atatggaaga tcctgaaaac tatgtctgca agcaagaatg gatcgacgag
     1981 ttatggaaag tagcacaacg agaattagct acaggttttt actacaatac accaactgaa
     2041 aacgaacaat tattcggtga acgtcgtaaa atcccagtat ataaatttgt cggtgaagta
     2101 ttatcttatg atgaggcaac acagattgct acgatacgtc agagaaactt gttttctgta
     2161 ggagatgaaa tcgaatttta tggtccagga ttcagtcatt tcagtcaaac cgttgaagtt
     2221 atgcataatg aagaaggcga atccattgac cgagcaccaa atccaatgat gattttaact
     2281 atgccagtcg cacagccggt gaaacctgga gatatgatcc gaaaacgtaa ataaggaaaa
     2341 agctgatata tccctgtttt aagcatcttc gcatacaaaa ttcaagaaaa gtgttacact
     2401 tgttttctgt agaaaaagga tcgttaaata aaggttttga ttgatctctt caataaggag
     2461 gaacaaaatg caaatatatg attacatcgt aattggtggc ggctctggcg gtattgcttc
     2521 agcaaacaga gcagggatgc atggcgcaaa agttttgctg attgaagcga atgaattggg
     2581 cggaacatgt gtcaacgtcg gttgtgtacc taaaaaagtt atgtggcaag caagtacgat
     2641 tttggaaaca attgaacgag atgcctcaag ttacggtatc caagcagatt taaaaaatgt
     2701 tgatttcgcc ggtctagtag aaagacgaga aaaatatatt gattttctac acggtgctta
     2761 tcaaagagga ttagatagca atcatgtcga ggctataaaa ggttacgcta cttttatcga
     2821 tagtcaaacg atcgaagtaa acggtgaaac ctatcgtgct cctaaaatct tgatcgctac
     2881 tggaggccgt gcaagtaaaa tgaccattcc tggtggagaa tacgcaattg attcaaatgg
     2941 attttttgaa ttaacagagt tacctaagag agcaatcgtt ttaggtgctg gatacattgc
     3001 ggctgaactt tcaggtgtat ttcgtggttt aggtagtgaa gtcatgtggg catatcgaaa
     3061 agaacgacca cttagaacgt ttgataaaat gctttcggac aatttgatcc aaatgtacca
     3121 agaagctgga ataaaaactt acgcacatca tattgctaaa gaaattacta aaaacaatga
     3181 cgaatatacg gtgctatttg aaaatggtga aaccttaact ggtgattgtg tactttttgc
     3241 cggtggacgt gtaccgaata ccgaaaaatt aggtctggaa aatacaaatg tggaattgga
     3301 caaaaaaggg tttatcaaag tagataaata ccaaaataca acggatgaac atatttttgc
     3361 tgttggtgat gtgatcggta aattagatct gactcctgtt gcaatcgcag ctggtagacg
     3421 tttatcagaa cgtttattta atggaaagaa agattcttat cttgactata aacttgttcc
     3481 gacagtcgtc tttacgcatc caccaattgc aacaattggt ttgacagaag aagaagcatt
     3541 ggaaaaatat ggcgaaaatg aattgaaagt ttatcgttca cgctttaccc caatgtactt
     3601 tgcattaaat gattatcgcc aaaaatgcga aatgaaattg atctgtgtcg gaaaagaaga
     3661 aagaatcgtc ggcttacatg ctattggtat tggtgtcgat gagatgctgc aaggttttgc
     3721 cgtggctatc aagatgggtg caactaaaga agactttgac aatacagtag ccatccatcc
     3781 aacaggagcg gaagaattcg taacgatgcg ataatttaat attaacagtt ccaacagctt
     3841 tttattgaac aaaccatgta aaaccagaaa atcagtatac tgattttctg gttttttgtt
     3901 ttttagttat ttatgtaaga ttgcttcgat aattctcaaa acgaaataag tagggaagtt
     3961 ggagattttg attatcttcg taactgacga agagtaaatg aatccatttg gtatgcactc
     4021 ttaagttcaa aatattggta tcgatgttta aaagaaatag aaatgtatcg aagaacagat
     4081 gtttaaaaaa gtgtgacttt gtataaataa attagaagaa gcaaacgagg atgttaaaag
     4141 aataccatat agttaccaaa ccaagaaagt tctcgtcaag ttcgctggta cgaatcaaat
     4201 caagtgaaaa aagggactgt gaaaaaaatt gtgtcatagt tagtcacttt ttccgaacaa
     4261 atagtgttca atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact
     4321 atttttccat ttgtttctct aatactcagg acaaaacact gattt
//
LOCUS       DAZAID010000155         4341 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000155 DAZAID010000000
VERSION     DAZAID010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.159"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..894
                     /locus_tag="ACG9BE_002786"
     CDS             13..894
                     /locus_tag="ACG9BE_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN3033483.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            907..2517
                     /locus_tag="ACG9BE_002787"
     CDS             907..2517
                     /locus_tag="ACG9BE_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN3033484.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2594..3253)
                     /locus_tag="ACG9BE_002788"
     CDS             complement(2594..3253)
                     /locus_tag="ACG9BE_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN3033485.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3536..4201)
                     /locus_tag="ACG9BE_002789"
     CDS             complement(3536..4201)
                     /locus_tag="ACG9BE_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFN3033486.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1158 a          853 c          864 g         1466 t
ORIGIN      
        1 agtggggttt ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag ctttggtcgt
       61 ttcgaagctt taaaagatgt ctctttcacc gtacattcag gtgaagtcgt tggatttatc
      121 ggaccaaatg gttctggaaa atccacgacg attcggacac ttttagggat tataaaaaaa
      181 gacggcggaa cggcaacgat ttttgggcaa gatgtatggg aagatagttt atctatccat
      241 gaacgtcttt cttatgttcc aggcgatatc tctttgtggg gaaatttgac tggaggagcg
      301 atcatcgatc tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa agatgaactg
      361 attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa actattcaaa aggaaatcgc
      421 caaaaagtgg gcttgattgc tgctttggct gttgattcgg atctctattt atttgatgag
      481 cctacttctg gtttagatcc tttgatggaa acgattttcc aagaagaagt tgaaaaattg
      541 aaagaacagg gcaaatccat cctactttct tctcatattc tcagtgaagt cgagcgacta
      601 gcggataaag tcgtgatcat acgcgaagga acactcgatg aagaaggaac actcgatgaa
      661 ttgcgtcatt tgactcgttc gaccatcact atagaaacag aagcagagaa tagtcactta
      721 gcacaattga acggtgtcca tgactttgtg caaaaaggtc acctcgctca atttgctgct
      781 gataacgaag ctttcgatgt gattttagct gaagcgtcca aattaggtgt gaaaaaattt
      841 gaatctattc caccgactct tgaggattta ttcatgagac attatgaagg atgaggagga
      901 agcgaaatga ctgataaatt ttcacgctgg tccacccttt tttctcaaaa ccttagccgt
      961 gactggaaaa agatcctgat atggctagtc ggtgtcactg ctttttctgg tggttttgtc
     1021 cctgcgtttg aagaattgac ttccggacaa ggattagctg gattatacga aaccttgcaa
     1081 aacccggcaa tgattgcaat ggtcggacca actccagctt cgaatgctgc tgagtatact
     1141 ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc tgctgtgatc
     1201 tctgttttac acgtggtgag ccaaacgaga aaaaaagaag aacttggtct gattgaactt
     1261 attcgttcct ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc tgaaagtatt
     1321 ctgatcaatt tgcttttagc aatatcgatc agctgtacga tgattttttt ccaagcggat
     1381 tcgatcactg cagaaggtgc ttggcttttt gggctatctg taggtagtat ggggattttg
     1441 gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag ttcgaatggt
     1501 ctgtctttag cgtttttagg tggactttac cttctacgag caataacaga tgtagctgat
     1561 tcctcccttt cgatgtggaa tcctttaggc tggtcttatt tgacctatcc atttacaact
     1621 aacaactgga ttcctttgct ttatttgttt ttgttcagtc taatcttgat aatcagttca
     1681 tttgttttag aggaacatcg agatttgaac accggctata tccccgaacg tgaaggaaaa
     1741 ccgtatgcta aacgttctct tttatccttg cgtggtttat tattctatct aaataaaggg
     1801 atgacaatca gttggcttat cacttttggt ttgatggggg cggcatacgg ctctatctac
     1861 gggaatatgc aacagttttt agaaagtaac gacttaatga aacagatgtt tatccaatct
     1921 ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg tagtcttgat ttgtctagct
     1981 gctattttac caattgctag catcaatcgt ttatttacag aagaaaccca cggacgtatg
     2041 aatcagctgt tcgcaacaaa aatttctagg aaccaacttt attggaccaa tatgttacta
     2101 gctattctat ttgctatatt ggcaatcctg attagttctg gtgcactagg gctttctgct
     2161 ttggcttcga tgaaggatgc gaaagacatg gatctattga tgtttcttaa agccgggttc
     2221 aatttcctgc ctgccgtttt agtgtttatc ggaattagtg gctttctatt cggatggctt
     2281 cctcgaatcg gtaaacttgc ttatatctac ttagcttact cctttgcact caattacttt
     2341 ggcggaattt tggatttacc agaatggttt tccaaaacag ctgtccctag ctgggttcca
     2401 cggatgccaa acgattcttt tgatctttcc gtgttcctta cgttatcatg tatcagcctt
     2461 gcactgttcg tccttggttc tattggttat aggcgaagag acttactaga aggataataa
     2521 aaaagcatgt acaatttcat gctagcctcc aaatctgggt caaaaatcca acatttggag
     2581 gtcatttttt tatttacaag tcttctaact gctctttatc aatgatccag acttctcgat
     2641 tctttctttt tacataacca ttctcctcta ataatttaaa ttttctagat aaagtttcgg
     2701 gggtcgtacc gataaatgca gcaagttctt tcattttcat tggcaggaaa aagtgatcct
     2761 tccctgtcgc tttagctaag tcaagcagat agagagcgac tcgttcttct actttttcca
     2821 tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt ctcaacgttg attgctaaaa
     2881 gtttcagact aagggcggga taatgcagta agagttccga gaaatctttt tgtctcaata
     2941 tgcaaatttc cgttttttct agtgcttcgc caaataaaga ctcatttgga acgcctagca
     3001 actggctctc tccctcatat ccgcccggct ctattactcg taaaagctgt tcctttccgc
     3061 tagcagataa ttgatacact ttcatactgc cggaggccac gatcactaat tgaggttctc
     3121 cattcggcgc aagaatctgt tctccttttt ccacatagtg atgtttgaca agtgcgtgta
     3181 ctttttcttg atcttgctgt actaagtgat tgaacaaagg aacaaatgaa acacataaat
     3241 gatcatgact cataatctct cctccttcta aacagctatt tctaataaag aaacataact
     3301 tcccttattc caactgataa ttttctttta tcatagcata aacgtcaagc gaaccagata
     3361 ccagcaaata gagtggattt ctttttgtcc cttgctttat tttccttctt ttgagacctt
     3421 aagtaattga ttcctatcaa ttttttaaat atttactttt tgaataatca aaaacaaacc
     3481 ttcactcgca agccgagagt aaggtttgtt tttcgcattg cttatttcgt gttgtctatt
     3541 gaaatttttc cgcacctagc ttttgatata gtggtgctag ttcttgtaag gtttcagcgg
     3601 ttatttgctc aacgtgttcg atttcttgcg gatagaagaa tagtttaccc atttgaaagg
     3661 ttcggtctct catcgctttt ttcaactctt cttgtttctt tgtatcggac aaatagtcag
     3721 acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa taatccttga
     3781 tatcgactgt tttgctccac tctttcagta tcgcaagcaa ctggttatac gctgcaatgc
     3841 taccatatcg ttcttcgctt ttataatgtt ggtacatcaa atagatttgg tattgttttt
     3901 gattcaacaa aactgctaga caggcattct cgtcttgacg agattcgctt cggtacgcgc
     3961 tccaaaaatg actgcgtaaa ttccagccat tcgtccagct ttcgatttta ggtttgctca
     4021 tgccgtacat atcaggcaaa agtgcagctg tctggagctg aatctctttc catttttgcc
     4081 agtgttcttt atattctttc ttgatttgtt caatcttatc tgttggatgg ctctctttga
     4141 gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata atctgatttt ccaagttcca
     4201 ttgtttcgcc tctttccgtt atcttttgta ttctacctga attttgcgat atggacaaat
     4261 agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca aaaaactgcg
     4321 atgagcatca ccgcagtttt a
//
LOCUS       DAZAID010000156         4336 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000156 DAZAID010000000
VERSION     DAZAID010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.160"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACG9BE_002790"
     CDS             127..579
                     /locus_tag="ACG9BE_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033487.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACG9BE_002791"
     CDS             593..1282
                     /locus_tag="ACG9BE_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033488.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACG9BE_002792"
     CDS             1407..3758
                     /locus_tag="ACG9BE_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFN3033489.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACG9BE_002793"
     CDS             3838..4233
                     /locus_tag="ACG9BE_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033490.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1526 a          548 c          867 g         1395 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttattt
//
LOCUS       DAZAID010000157         4306 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000157 DAZAID010000000
VERSION     DAZAID010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.161"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..923)
                     /locus_tag="ACG9BE_002794"
     CDS             complement(27..923)
                     /locus_tag="ACG9BE_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3033491.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(916..1596)
                     /locus_tag="ACG9BE_002795"
     CDS             complement(916..1596)
                     /locus_tag="ACG9BE_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3033492.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1597..2379)
                     /locus_tag="ACG9BE_002796"
     CDS             complement(1597..2379)
                     /locus_tag="ACG9BE_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN3033493.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2372..3289)
                     /locus_tag="ACG9BE_002797"
     CDS             complement(2372..3289)
                     /locus_tag="ACG9BE_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN3033494.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3453..3707)
                     /locus_tag="ACG9BE_002798"
     CDS             complement(3453..3707)
                     /locus_tag="ACG9BE_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033495.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1261 a          944 c          754 g         1347 t
ORIGIN      
        1 tactagttct cactaatctt gtataattaa accctccgca acttgacgga gatggagaag
       61 atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac
      121 aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa
      181 cgctggaaga ctttttcaac agaggtttgc gttcctggtt tgataggatt ggccactgtt
      241 aatcgaatga atggatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc
      301 aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca
      361 gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca
      421 taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt
      481 tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat
      541 cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc
      601 tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa
      661 aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt
      721 tcattttcat ccaaccatgt attcagtgca tctactaggt ccattagatt cttttctcga
      781 aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta
      841 gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg
      901 agaagcaaca gccatttaat caaatctaaa ccccactccc cacaccgtat gaatatacac
      961 agcatgtgcc tttgctagct tccctcgaag attggaaata tgcatattca ctgttttctc
     1021 actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt
     1081 tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc
     1141 cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat
     1201 gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg
     1261 taacaaaagt tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa
     1321 caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga
     1381 tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag
     1441 aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc
     1501 atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaggaatgaa cagcttcatc
     1561 gtcttcgata atcaaaatag tttccagcag gaccacctac ttaatgtcag atttttggaa
     1621 aataaacatt cctagagctg atgataccac gattgttact agactgacca gaatcatttt
     1681 cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt
     1741 aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaatgaaggc
     1801 cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa
     1861 tgtcaaagcc actggaacag ataatgtccc taatgtcagc agaagcaata caggaaatac
     1921 ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag
     1981 tgtgtaagca agcatggcta gtcccgtgat caaaaccacg ccaagtgttg tgatcaaaag
     2041 cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt
     2101 gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc
     2161 cagaagaatc catgcagcca ttttttctac cattaaaatc cctgttgcac cgttgatgac
     2221 tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca
     2281 agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt
     2341 ccatactttt attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt
     2401 gtaagaaata atcttccagt ttttgcccaa ctaggtgaat accatccaca ggaacttttg
     2461 cttctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa
     2521 cacggctaga ttgtacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt
     2581 gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt
     2641 tcgataattc tttgatcaag tgtccttcat gcaaaatacc atagtgagtg gcgatctgtg
     2701 acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac
     2761 gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat
     2821 ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca
     2881 tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca
     2941 gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat
     3001 ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc
     3061 ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca
     3121 aacccataat agttctcata aaggtagact tacccgctcc attttcccca atcaatccat
     3181 agatcattcc acgtttgatt tcgattgata ctttatctaa tgctttggca gcaccgtatt
     3241 ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc
     3301 tttcttattg atgagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata
     3361 aagaaagtgt aaattttcca attgaatgtc aaaaaaaaca atcggacaat acgtgtcttt
     3421 cctattatca ggcagtttgt tatacgcttg tatcagtttc ctgattcttt cttttcgacc
     3481 aaagtcgttt ctctgtattc ctcttcactg aaaatttttg cttctttcag atcaagcttg
     3541 agtaatttta ccagaccggt tataaggata actatagcaa gactccctcc cagaaaaatt
     3601 agactgtctc gcagacgtgg ttgcgttgtc ttccaatcaa agagcgcacc aaacagtgct
     3661 aatccaagtg gactggccac catggtaatc gttgttatcg ttgacataag tcgtccttgt
     3721 atatgcgttg gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat
     3781 ccttaaaaac ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca
     3841 aataagaggt tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc
     3901 ctgccaactc ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa
     3961 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa
     4021 tattgtttga tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga
     4081 ccaagaatcc tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg
     4141 aaagtgccag aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga
     4201 ttctcgtaac tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct
     4261 ttacgggctt tcttaagacc tggttttttc ttcgtatcat tgaata
//
LOCUS       DAZAID010000158         4298 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000158 DAZAID010000000
VERSION     DAZAID010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.162"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..803
                     /locus_tag="ACG9BE_002799"
     CDS             102..803
                     /locus_tag="ACG9BE_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN3033496.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1375..2391)
                     /gene="tsaD"
                     /locus_tag="ACG9BE_002800"
     CDS             complement(1375..2391)
                     /gene="tsaD"
                     /locus_tag="ACG9BE_002800"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFN3033497.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2388..2852)
                     /gene="rimI"
                     /locus_tag="ACG9BE_002801"
     CDS             complement(2388..2852)
                     /gene="rimI"
                     /locus_tag="ACG9BE_002801"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFN3033498.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2859..3401)
                     /gene="rimI"
                     /locus_tag="ACG9BE_002802"
     CDS             complement(2859..3401)
                     /gene="rimI"
                     /locus_tag="ACG9BE_002802"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFN3033499.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3385..4107)
                     /gene="tsaB"
                     /locus_tag="ACG9BE_002803"
     CDS             complement(3385..4107)
                     /gene="tsaB"
                     /locus_tag="ACG9BE_002803"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFN3033500.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1271 a          916 c          741 g         1370 t
ORIGIN      
        1 aagtattaaa acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa
       61 gtgacaataa atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg
      121 attttcactt ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt
      181 tccaacagat caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg
      241 acgatggtga tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac
      301 gttatgatga aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc
      361 ctatgtcagg aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata
      421 ttgaaacatt ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac
      481 agttgatcta tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc
      541 aaatcggatt gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag
      601 aagaattggg cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa
      661 aagaattagc agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac
      721 tacgtgattc cggactgtta gattatgaca aaaagcgttt tactttctgt aagacagcta
      781 ctactccagc tatgagagaa taacgcgaac aaaaataagc agtgaccctg atatttatca
      841 gggtcactgc ttattttcct ccctaaaact tctgaaagat ccggcagttt tagatcactg
      901 ggcaagactc aaagattttc tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa
      961 aatactgata gattaactag aggaagcgtc ttgtcctcat ccgacctaga ttattcgata
     1021 ttcctcgaca ataaacgatt aatcagtgag ggcaatcaga ggaacggata atgatgaata
     1081 agctgtaaaa aatagggctt tgacaaaagc gtgtcacagc ccttatttcg attaaacggt
     1141 gtgtacctgt caactcttcg gtcacaagtc ataatgttca taaacatgag gagctgtttt
     1201 ttgaaattgt tccttgtgaa tcagagttaa acgacaggct tacgaactct tgattaaacg
     1261 gcgttcaaaa gcttgcatac gcggtaataa gaacagccag gtcaaaaaca tgagaagccg
     1321 tttttcctgg gctgttctta ttacttgatg cagggcagat ttttcacaac ctctttattc
     1381 agataaaacc aagcttggat cagcattcaa ctgatagcta gcaaaatttc ttttttgcat
     1441 ttccacatgt gctgctgctc caatcattgc agcattatct ccacaaagtc gaagaggggg
     1501 aatcactagt tctacctcag ggagtttagt tgccaaagca tcctgcaatc cttcacgcaa
     1561 cccttgattg gccgctacac ctccagctac aatcagttgc ttgactggat aattttgaca
     1621 agctcgaagt gttttagtaa tcaatacatc gatcacactt gcttgaaagc tagctgctaa
     1681 atcatttttg tctagatctt ctccacgctg ttgtgcatta tgcaccaaat tgataaatgc
     1741 actttttaat ccgctaaaac tgaaatcata attatcttca tggatcatcg ctcgtggaaa
     1801 atggtaatta tcttttccct gatgagctag ctgatcgatt tcttttccgc ttgggtaaga
     1861 aagacctaat actcttccaa ctttgtcata agcttctcct gctgcgtcat ctcttgtctc
     1921 cccaatgatt tcatatgatc catcttcttg catatagact aattctgtat gtcctccgct
     1981 aacaagaagt gccatcaatg gaaactggaa tggtttaacc agacgggctg catagatatg
     2041 ccctgccata tggtttacag gaataagcgg caattggtgt gcccaagcaa aagccttagc
     2101 tgcagaaata ccaatcagca acgagccgac aagtcctggt ccataagtga cagcgactgc
     2161 cgataaatct tctgccgaca caccagcttc taccagtgca tcttccaagc ataaggtgat
     2221 ttgttctacg tgatggcggc tagctacttc aggaactacg ccaccaaaac gtttatgact
     2281 gtttatttga gaagcaacga tgttagataa gatctctgtt ccatttctaa cgaccgctac
     2341 actcgtttca tcacaacttg attcaatcgc cagaatcaat tcttcattca tttcatcgtc
     2401 tccgttttta actttctgct cataatgatc gcatcttccg tcggattgcg ataatatgat
     2461 ttccgtttac ctaatatacg aaacttttct gattcataaa gttttcttgc tgcttcatta
     2521 gatagacgta cttctaaaaa aacatgcagc gaatctttta tttgttccgt aatcagtaat
     2581 tgtaacaaat gccgtgcatg cccttttcct tgttctgaaa ctgcaacagc aatatttgtg
     2641 atctcgactt catctattac tttcgagtat ccaataaagc cacagatctg gttatcttca
     2701 actaaaagca gataatcaga gtgaggctgt tggatgtctg ccaaaaactg cattttcgtc
     2761 caaggtgaac catgtggata agcattttcg ctaatcatcc atacgcattc tgccaagtac
     2821 gcttcatcaa aatctttttt tctgcaaatc aactaatctc actcattttc taaaagtttt
     2881 atcatatcca acgcatcttc ttttgtttca gtgtaatatc tttttttgat cgttgttgct
     2941 tgaaaaccta acttccgata aagtctttgt gcatcttgat tactcatacg cacttctaat
     3001 gacatcgtct cacagcgatt cattatagca aagttctcga tttcgtcaat aagcagcgaa
     3061 ccgattcgtt tacgctgata tgccggtcga acggcaatat tcgtgatatg gcaatctaaa
     3121 ccgaacgctc ttgaaccgat aaaaccaatc attcttccat tatcaaagat acctagatat
     3181 agatgaggaa gcggtgactt caattctgat ataaaggcac tttttgtcca tggcaattcg
     3241 ccattataga cttctcgttc gacatccagc agctctttga tatcttctgt agtcaattct
     3301 cttagtacgt aacgacgatc gatttgttcg attgtttttt ctgtataaat cattttatct
     3361 tgaaaaaagc ttctggcata cgctctaaat tttttcaaca taactttcgt ctccaggtgt
     3421 gtgcgtctcc aaccattttt cttctgcttc tacccgtttt aaatagcgag gaagaaaagc
     3481 ttgtttctct gcaactggtt cagctaaagc acctaattga gccacagtta caccacttgg
     3541 aatatcccat gctggaactg tattaatcat cgcactaggc aatatttcct ttatttcata
     3601 gaagaatttg tgacaatcac tcccgacgaa ataaatagct tcattcagct tttgtagttg
     3661 tgtgaacaaa tcagaagcag aaatatgtac atcttcaagt acaatttcta atctattttc
     3721 tttccagcgg tatgccccag cgtatacatt ttttcttcga gcatcaaaca tgggcacaat
     3781 aattttatcc acaccgatgc aattagccgc gatggttttc agactagata ctcctactag
     3841 ctctttatct aatgtataag caatcgtttt tgctgtcgtt acacctatac gaagccctgt
     3901 ataagaaccg ggaccatcag aaaccgcgat tcggtcaatg tcttttggac ttatttgaag
     3961 atctgcaaac aattggtcga ttgcaggcat cagtgttaca ctgtggttgc gtttgatatt
     4021 tgtttgtatt tgtccaagaa tttgttcgtc ttctaccaca ccgatcgcca aagtttggtt
     4081 tgcagtatct attcctaatg taatcatata tttgatcctt tctgctatga cattctcgcc
     4141 ttccattgta gcatactttt ttattctgag gatgaaaata ccaaaggcaa aactagtaca
     4201 atgatacatg attatctgct ggcgggcaaa ttttgcccct tcctgaaaca caaaaaagct
     4261 gcggtatcac ttcatttccc cacagctttt ccatcttt
//
LOCUS       DAZAID010000159         4277 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000159 DAZAID010000000
VERSION     DAZAID010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.163"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..528
                     /locus_tag="ACG9BE_002804"
     CDS             <1..528
                     /locus_tag="ACG9BE_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFN3033501.1"
                     /translation="PRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQW
                     WALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLP
                     LELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLL
                     NRIDKEILKVGNIDE"
     gene            521..1948
                     /locus_tag="ACG9BE_002805"
     CDS             521..1948
                     /locus_tag="ACG9BE_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFN3033502.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            1950..3020
                     /locus_tag="ACG9BE_002806"
     CDS             1950..3020
                     /locus_tag="ACG9BE_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFN3033503.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            3007..>4277
                     /locus_tag="ACG9BE_002807"
     CDS             3007..>4277
                     /locus_tag="ACG9BE_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN3033504.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICI"
BASE COUNT         1469 a          523 c          674 g         1611 t
ORIGIN      
        1 cctagaatta ataggaataa cacaactgca tttaaaattt ttagagctat aattaatcgt
       61 tgtctgagga tgattaaact tcccccaaaa attagacacc ataaatttga tttgtatcat
      121 ggttctcaat ggtgggcttt gtcgaaagaa tgcctaacag aattaataca aatgtatgaa
      181 caaaatcaac aagactacct gaattttaaa ataggtatgt ttgccccaga tgaaaaattt
      241 tttcatacac ttttttttaa ttcctcgttc aaaaataaaa atgttattgg aggaccagat
      301 ttaccacttg aattgaaaaa tattgaagaa actactttac aaacttcgaa attagcaaat
      361 attcatatta ttgatccttc catgaacaaa tggtttgatg aaagggatat agatcaaata
      421 aaagaaagtg aaaaattatt tgtaagaaaa gttaaatcgg attactctga taaactatta
      481 aatagaattg ataaagaaat tttgaaagtt ggaaatatag atgaatagaa tagtacctgt
      541 tttagagaag agtttattaa gtcgagatgc gtttcgagta ataaatttaa tcatttgtct
      601 gctttatttt atgcctagta cttttaatgt aactaatatc ccgatgaaaa tagcttttgc
      661 ttggggtgga attatagttt tgtatgaggt ttttataaag aaaaattttt ttaaaatgcg
      721 tttttcttgg ctactgattg gagcaatgat tagttttatc ttaactataa tcattaacag
      781 agaaaatttt ttaattccat ctatttataa ttttggatat ttgctgatta ctttgatagt
      841 aatttttcct gcagactttt cacaaagtga agaggaaaaa aagaaaaaaa tggtgtattt
      901 taataatgtt ttcattatca taattttttt agctgcgcta atatctatct atcaatttat
      961 ttttttgatt tcttatcatg tcccaacagg aacgccagga ttacttgcaa gacaaggttt
     1021 tattgaacat cggttatttg gtgtctatac aagtccaaat gttggagcta tgtttggata
     1081 tacttctatt attttaagtt taattagtgt attaatttca aaaataacaa aaacgattcg
     1141 tgttttttat atgatgaatt ttatcgtaca aatattttat attacacttt cttcgtctag
     1201 aggtgcccag gtagtaattt gttctttttt agtattattt cttttgttgt ttgccaattc
     1261 aaaatttaga aagagcatta gagaagtact acctgtaaaa atcaaatgga tggtaagcat
     1321 attattagtt ctattggtat atttttcttt tggtactaat tatgctaagg attttttagc
     1381 aacaattcct gtatcgattg atagaaaagt agaagttata gagtcaaatg aatcagataa
     1441 aactccactt ccagaaaaag aagtaatatt acagcattct agtgataact ccgaaatttc
     1501 agctggtcgt tttacgattt ggaaagctgc gcttaaagtt ataagaccaa ctttgttatt
     1561 tggttatgga gagaccgatt tctatgggaa tagcgatgca aaatttttga aaaaagtaga
     1621 tcttgatact acagatgtga atgaactaaa aagagcacat ggaaacatgc ataatggatt
     1681 tcttcaagta cttgtatcaa gtggtataat tgcttttata tgtatctatg ctttctatgt
     1741 gttaaatatc tttagtttac tcaaaacatt ttttagaagt aaagtgaatt atgcgtttct
     1801 tggtattgtt ttaattttta tactatctat ttttatagat gaaatggtgg aggctcatgt
     1861 acttttcaat aagcgcgatg tcatttcaat tgttttctgg tattatttgg gaagcatttc
     1921 ttacttgtat ttaaaaaagg agaattaata tgaagacagc tttcattgct tggactcctt
     1981 tacatttgat taatatatta aatgtttatc aaaattttta ccgagaatca aaaggagatt
     2041 tatttattta ttctgaattt gaaaacgcta gaagatatag tgagcgtata aaagaattag
     2101 ggctatttaa tgatgttgtt tttgtgaatc accggaaaat cggttcttcc tttaaacgta
     2161 aaattaactt gttaactaac cataatcaat ttttctcgga tgatttatcc gtttatgatg
     2221 aaatttttat acagggggaa aattatttca caaaaatact tttctccaat gtttacaaaa
     2281 aaaatccaga tgtaaaacta aattatatag aagatggttt gggtacttat ttgaagaaga
     2341 gtatattttc aacaagtaaa aacgttcaaa gagtttttaa aatattaaat aaaaatagca
     2401 tcttcaaaaa aaaaattgac gattattttg tctattgtcc ggaattgtta ctttatgatg
     2461 taaaaaatat aaaaaaaatt cccaaaatag atgaaaacag taaattgatg aaaaacatac
     2521 aatcaatctt tgggatagaa gaagtttttg tcggagataa atgtatcttt tttgatcaac
     2581 ctttattatc tgatggttat ggcatagatg aaacagatat tttcgctaaa gtaaatagta
     2641 tattttctgg aaaaaaagaa actttaatta agcttcatcc tagagcaagg acaaatcgtt
     2701 atggtgaaaa aaacatattg aattccagtt taccgtttga agtcttgcta cttgaaaatg
     2761 acttctcttc aacacttttg attagtccta tatctacaat ttctttttca ccatctatga
     2821 tgtttgaaaa gacgatcaac tctattatgt tggctaaatt aattttaaat gaatatacag
     2881 cattatctga agataaaaaa gaaacattga caaatattgt tcttttctgt gaacattata
     2941 accgtctatc agaagaaaaa attttactcc cagaaaactg ggcagaatta agaaagttgg
     3001 tagagtatgg gattatttaa cacactttcc aagtcaaaat tagttaagtc cagtttctgg
     3061 tatactatag ggaatttttt tattaaggga atttcattca taactattcc tatttttgta
     3121 aatattacta ctcaagcaga atatggtttg attaataatt tcacttcatt tgtatcaatt
     3181 ttttcgattc ttgttgggct aagcctaaat tcggcaatta ataatgcaag ttttgactat
     3241 aaagatgaca taaatgaatt tatgtcatcg gtactttctc tagctacact ttcgttaatt
     3301 gtgttcttat taatagggaa catatatttt ttgttttcaa atgctttttt tgatatgaac
     3361 caacttgtat ttaatttgat gttattacaa agttttgcta gttttgtaat tagttatatc
     3421 cagtcttatt ttacgattaa ctttaaccat tttaaatatt tgattttatc ttttatatct
     3481 acttttttga atattgcaat ctctgtagct ttgatgcaga cattatattt tgatgataaa
     3541 tatgttggca gagtagtcgg cggaacactg tcatttgtgt tgataggttt agtaatttat
     3601 ttaagaataa tgataaaggg tgctcatttt tttgataaaa aacagtggag atacgcttta
     3661 aaaatctctt tgccgctaat cccacattcc ttagcaaata tagttttagc acaattcgat
     3721 agaattatga ttaattctta tttaggtcct gctgcggggg gaatatattc ctacattagt
     3781 aacataggta ttattctaag cgtaatatgg atgtctacta ataacgcctg ggttccatgg
     3841 ttctatggtg agatgagtaa aaataatgaa aaaaaagtga agaaagttag taactattat
     3901 ttagtagtct ttactttgat tacgatgatt gtaatggtag tttcgattga tttagcaaaa
     3961 tttatggctc cgaaagacta ttattctggg ttatctttag tggttccgat aaccttaggc
     4021 tatttctttc agtttttata tagtttacca gttaatgctg aattttatga gaaaaaaaca
     4081 tcgtttattg cgattggtac agtagcaagt gctatagtta atatactatt aaacatgatt
     4141 ttcataccta ggttaggaat aattgctgct ggatacacaa ccgtatttac gtatttttta
     4201 ctttttattt ttcattataa tatcgctaaa aaaattacga ataaacaatt atttgacaca
     4261 aaaaaaatat gtatcat
//
LOCUS       DAZAID010000160         4124 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000160 DAZAID010000000
VERSION     DAZAID010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.164"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..202
                     /locus_tag="ACG9BE_002808"
     CDS             <1..202
                     /locus_tag="ACG9BE_002808"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033505.1"
                     /translation="YIIYASVLMSNYKRTKVKNYIYLLAYFFFIIVCLIFINIRGTAF
                     VNGILPLFRENIIFKAVYYFFE"
     gene            239..1177
                     /locus_tag="ACG9BE_002809"
     CDS             239..1177
                     /locus_tag="ACG9BE_002809"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFN3033506.1"
                     /translation="MGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLN
                     QMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCIT
                     GNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPN
                     ELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKG
                     NKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQ
                     LAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            1429..2439
                     /locus_tag="ACG9BE_002810"
     CDS             1429..2439
                     /locus_tag="ACG9BE_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HFN3033507.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAYID
                     SNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSN
                     ELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERD
                     FTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVET
                     IEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYY
                     LS"
     gene            2807..>4124
                     /locus_tag="ACG9BE_002811"
     CDS             2807..>4124
                     /locus_tag="ACG9BE_002811"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFN3033508.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYF"
BASE COUNT         1409 a          539 c          736 g         1440 t
ORIGIN      
        1 ttatattatt tatgccagtg tattaatgag taattataag agaacaaaag taaagaatta
       61 tatctacttg cttgcgtatt tcttttttat cattgtgtgt ttgatattca tcaatataag
      121 aggaactgct tttgtaaatg gaatcctacc attatttaga gaaaatatta tttttaaggc
      181 tgtttactat ttttttgagt aagaataaat agcacttaaa aatagagatg agggatttat
      241 gggaagacaa gaattatata gtttagctat caatgaattg gaaaaaagaa atcttgaaat
      301 acatgaatta aatcaaaaaa ataattctat tttaggcatg aaaatacgaa ataaaattaa
      361 tcaacttaac caaatgaaaa aacgagataa tctgttgcca gcaaaatata cccttaaatc
      421 gatgaccggt aaggggccta ttaaagacta ttcaaacaga gagaatcgca gtgatgaact
      481 aagaaaaaaa gagttaacta atcaaggaac acctaaaagg attgctgtgt atagttgtat
      541 cactggtaat tatgattcta ttaaagaacc ggatttttct catccattgg tagattacta
      601 tttgtataca gataatgtga ttacaagtga gcattggaat attagagaga tacctaaata
      661 tatatcggaa ttaaaaaata atattttgat caatagatat attaaatttc atcctaatga
      721 gctatttact gactacgatt ttactttgta tgttgatggt aatgtaacaa taacttctga
      781 tattaccgga ttactgaaca gttcttccaa acttgggtta agtatgcata aacatagtga
      841 aagaaaatct ttatattctg aggcagatgc attaattgca gttggcaaag gtaataagga
      901 aagtattata gagcaaatta gtaagtacag agaagaagga tttcctgata attttggatt
      961 ggctgaagcc acggttattt tagttgattt aaataattca atcgctgaga atcttctaga
     1021 tttgtggtgg agagagtatt tgaactcaga aagtgggcga gatcagctcg catttcctta
     1081 cgtgttgtgg aagtccggac atgcaatgaa ggaagtgact accttaggca ataatgtata
     1141 taaaagcgct aaattaagaa tcataagtca ttattagccc taaatgattt ctaaactatt
     1201 ctttcaaatc ctagtaaata tttggagata gaagatttcc tttttaaagt cataaaaaat
     1261 taagttatat tttttgagtg aatggagaag acgcatagta tcataaactt cgttatatct
     1321 taaattatgg ggtatgccta aataataaat gtaatacgag tataaagaag aaagcaattt
     1381 tgggtttaac cctattggtt cctttcagaa aaaggtggat gaataaaaat gaaaatatta
     1441 ataacaggtg gagcaggatt tattggtttt catttgtcta gaaaattact caaggaaact
     1501 gaaaatcaaa ttgtagcgat tgataatctc aatgattatt atgatgtcca attaaaagaa
     1561 gatcgattaa cgatactgta cgattataat aattttaaat ttatcagaat ggatctatta
     1621 gagaaagaaa aattagattc tctatttgaa agtgaaggtt ttgacatagt aattcattta
     1681 gctgctcaag caggtgttag atattctatt gaaaaccctt atgcttatat tgattctaat
     1741 attatcggtt ttcttaatat tcttgaagcc tgccgaaact tccctgtaaa gcatctatta
     1801 tatgcatcat ctagttcagt atatggtggg aacacgaaag taccgttttc aacagaagat
     1861 tcggtggatc atccagttag cctttatgcg gctaccaaaa aatcgaatga attaatggct
     1921 catacttata gccaccttta taaaatacca acaactgggc tgagattttt cactgtctat
     1981 ggtccatttg ggcgaccaga tatggcctat ttctctttta ccaagaatat tttatctgga
     2041 aaagaaatta agatttttaa ccatggaaag atggaaagag atttcactta catcgatgat
     2101 atcgtagagg gaatcactag attaatcaat aaagtacctg aacccaactc cgactggaca
     2161 gacatcgatg atttgagtag tgaaagttgg gcaccatata agatatacaa tattggaaat
     2221 aataagccgg ttgcattgtt ggagtttgtt gaaacaattg aaaagtattt aggtgttaaa
     2281 gcgaacaaga tttttttgga catgcagccg ggtgatgtcg ttagaactta tgccgatatt
     2341 gaagatttag aggaaaaaat caactttaaa ccgaatataa ccttggatga gggcattaaa
     2401 aaattcgttg aatggtatac agtgtattat ttgagttaat tttgaaagga atattgaaga
     2461 tgggtaattc atacgatttt tttagctttg ttaggtattt taggaaattc tactttcaaa
     2521 cgtgacgaac tttctcgccg aatagatatt ttctataact aaaagaattt ctataaaaca
     2581 aaattgtgta tcaagatgtg tgaactggat tctgactgcc aatattgatt gatgagattg
     2641 aagaaatata tagttgatca tggatatctt tatttgaaaa gatcttagct attcacatat
     2701 tcaaaaaaat ttttactacg aggactcaca cgatacagag gaatttaggt agttctaatt
     2761 aaaccagaaa gtcatctata gatgtgagtt gagggaggtc atacggttgg atagtagcag
     2821 taataaatca cattcaatag ctaaaaaagc ttttatttac gcaataggaa cattatcatc
     2881 aaaaatgata tctgtcatta taatccctat ctatgcagta tatatttctg ttgccgattt
     2941 aggaagcttt gattatcaaa atacaattgc tcaatttctt tctccagtta tttttggagc
     3001 aatatgggaa gcaattttac gcatgggatt aggcaaagaa aaaaatattc agaaaaaagt
     3061 agtgacgaac agtatttgtt ttgttatagc aaatacagtg atattagcca tttttttacc
     3121 aataatatat agaatgattt tcccgaattt agagtattat caattgtttg tttttcaatt
     3181 aattttcact cctatcctaa gtgtttttca atatattgta agagcactag gtgaatcaaa
     3241 gatttttgtg atgagtggta taatcgctgc gggtatcaac ctgttactcc ttactatttt
     3301 tgttgttaca atgggtatgg ggatacttgg tctcttactc tctatcataa tttctaatgt
     3361 tataaatttg atttttttga ttttcagggg gcagatcttt caatatttcg caacgagttc
     3421 ttttgatttt ttatttttaa agtccatgct gaaatactct atcccactca ctttgaatct
     3481 cacttttgga tggctaatag ttggtttttc aagattttat attggaacgt acatcggagt
     3541 aacagaaaat ggtatttttt cttttgcgat gaaattttca tctataattt taactcttac
     3601 aagtattgtt agtatgtcta tgatagagga tgcggttatc acgtctgaga atgatgattt
     3661 catcgaaaga ttcgttgaga aaaataccta tatttttaaa atgtacatta ggttagcgac
     3721 tctttctatt cctatcattt gctgtgcata ctcttttata accaaccctg attataaaaa
     3781 atcattagtt ctagttccaa tattagttat tatggcatgt ttcagtggga tgacaacaaa
     3841 tatcggaaat atatttgcag tatacaataa aacaaatcag ttattaatga tttcagtagt
     3901 gtcaggtcta ttgagtgttg ttggcaccta tttattaggc tattcttttg gagtaattgg
     3961 tgtctcattt gctactttgc tcggtacttt agtattattt ggacttagat attattttgg
     4021 aaggaaaatt gcttattatg catttgattg gaagcgaata tcgctacttt tagttctcta
     4081 ttccgcaatc gcttttgtat gcattcatag aggcttctat ttta
//
LOCUS       DAZAID010000161         4119 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000161 DAZAID010000000
VERSION     DAZAID010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.165"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(23..1789)
                     /locus_tag="ACG9BE_002812"
     CDS             complement(23..1789)
                     /locus_tag="ACG9BE_002812"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3033509.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1749..3503)
                     /locus_tag="ACG9BE_002813"
     CDS             complement(1749..3503)
                     /locus_tag="ACG9BE_002813"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN3033510.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3613..>4119)
                     /locus_tag="ACG9BE_002814"
     CDS             complement(3613..>4119)
                     /locus_tag="ACG9BE_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFN3033511.1"
                     /translation="FSIMVSSIGGAFGHSISPGIFGLLSGIIFVYISYNYPRKGIFTI
                     YTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVASWALMQLGSAA
                     GQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSMIGVLIGR
                     KVLKKYKK"
BASE COUNT         1382 a          790 c          583 g         1364 t
ORIGIN      
        1 ggttaattta aagcctattt catcatcgtg attgagcttc tttttcatag acgaatttct
       61 cagatagttt agttttcttt atgagatttt tgtatatttc tgaccttcga tacaactcat
      121 tatgagtacc ttgatcaact agttttcctt gatccatcac tacaatttta tttacctttt
      181 caatagactt caagcgatgc gatataatta gtactgtctt atcttgtatt aaacgattta
      241 aactttcctg aatacgtttt tcattttccg catctaaact ggcagtaatt tcatctaaaa
      301 ttaaaattgg cgcatttttc aaaaaagccc gtgctatgga taggcgttgt cgctctcctc
      361 cagaaagtga acttccgttt tctccgacca atgtatgata accgtcaggt agtttttcaa
      421 taaagtcttc gcagttagct aaacgagctg cttttttcac ttcctcatct gacgcttgac
      481 tatttccaat acgtatattt tccaaaatac ttgtattaaa caaagttacc tcttgaaaaa
      541 caatagcgat tttgctaaac aaagaagctg gagatacacg tttaatatca tacccatcaa
      601 ttaaaataca gccttcatca taatcataca gtctagacac taatttcaag atactagttt
      661 tcccggaacc actagcacct actaacgcag tcacttctcc ttgtttagcc gtaaaagaga
      721 tatgatctaa aattggggta ttgttatcat atgagaaaga gacatctcgc aactcaatgt
      781 caaaagattt cagaggacta tctgatccct cttggatact ggtttccttc atagctctaa
      841 ttcgctgtat tttaggagct aaataaaata tttcaagcac tgcttctttc atagaatcga
      901 aggaatcctt tatttttatt gcggctagta agtaaccaac gacgtagagt atagtcactt
      961 cacccgttaa cagtaagtga acacctacta gaatgactac tgctaaacta acatatgaga
     1021 aaatagagga taaggccatg actgaaaaag tacttaattc tacctttaaa tgaatccttt
     1081 cactctcttc catttttttg aaaagtctgt cttgaacttt tttagataga ttaaagctat
     1141 taatctcttg ctgcaattct atagtttctt gaaattcttc cgagttctct ctcaaagtat
     1201 cgtaatattt agtattggct ttcgtttggg atttctttga taacaagatt aagacaaaac
     1261 taaataacgt tggcaaaata acagctaatc ccattttgac attaccaacc aaaagcatca
     1321 ctgaaataaa agggaaaaac agagccatac cgccggcttt aggtattgca tgactcatcg
     1381 catgctcaat accttcaacg tcagacatga tagtttgaga taaatctgat aaattatgtt
     1441 ttgaaaaata ggataatggt aaatttgaca aatcgtctgc aatttgcact cttaaatgcg
     1501 cactttcttt ataagtacta ttatataagt tttcatactc cctatctaac aaccaataca
     1561 tgaaaagcaa agtaacaaac gatacggcaa aataaagcca acggggcttt gtattttcta
     1621 aaactaattc gtctatcagc atcattataa tgaatgctgg taccatatta atgcagtata
     1681 caaaaaaact agaaatagaa gctttagtta aagcttctgc gccctgatca cttaaagcaa
     1741 atttgttttt ataaaaactt ttcattgtca agcctccaat catttgcatt ctcataatta
     1801 gtgaccaatt gtttatacac ttgatgttta gccattaatt cattatgatt tccacgttct
     1861 actattttcc cttctgacaa gacaagaatt tcatctacag atttaatgct ggataatcga
     1921 tgagctatca taataactgt tttttctttc atcagatgtt gaaaagcctt ttgtagttca
     1981 tactcattat ctggatcaat agatgcactg gcttcatcca taataacaat ctttgcatct
     2041 tttaaaattg ctctggcaat agctatacgt tgtttttctc ctcctgacag ataaactcct
     2101 ttactaccaa ttaaggtatg ctctctttcg ggaaattttt ctaaaatcga ttcgcaaccg
     2161 gctaatctta gcgcgtcaaa tactttactt gctggcgctt gaggatttgc tatagaaacg
     2221 ttatcgtaaa tactctgatt gaatagtttc gtatcttgaa agacaaaagc aattgtgtta
     2281 ataattgtgt cttttgaata gtgatttaaa ggcttaccac caattttaat ctgtccctta
     2341 tccacattgt agaaacctga aattagttta gctatcgtac tctttccgct tcctgatttt
     2401 cctactaatg cataaattct tttttgttct aaaactaagt tgaaattttc taaaacacac
     2461 tcttctttgt atgaaaagct gacattttca aatgcaatgt cacaattttt aatctgtgtc
     2521 tcgttcccat attttagttg gtcttgcttc atttctaaat aaagggagtc tagtgtttcg
     2581 actgcatatc ttgcttcaaa caagtgcata cttacataca ttattctcat gaaagaaaca
     2641 aaaataattc cacttaaaaa aaacaacatc aacaagtcaa cagctaatat cttgggagtg
     2701 gtaagagaag ttataaaaaa ggttatagga actatcaaaa tgcaaactat gccaaaaaat
     2761 agccattgaa aaagaacaaa aggtgttttc ccactcaaag aatacttata tgcaaactta
     2821 gcataatcgt taattgcttt attcaaggct ttaaatgatg ttactttagc accaaatatc
     2881 tttataactt gaattccgcg gatatattct accgtttcag agcttaacac atctaacgat
     2941 tcttgatatt tttgaataaa atttgtattc tctgtcattt tctttaacaa gaatacacca
     3001 attactaaca tggcgattag aaccaatcca attttaaagt taatgaaaaa gcctaaggca
     3061 actgataaaa caggtgttac tatggcttga gcactatctg gaatcaagtg agcaattgcc
     3121 atatgtgttt tagccgcgtt gtcatcaatt gtctttcgaa tatatcccga agaatttaaa
     3181 tcaaaaaaac gaaaacttgc ttctgttaaa ccgctaattc ccttttttct caaattagtc
     3241 tctaagcgaa acccaaggat atgagaaaat aagccagaaa aaatataaaa taaagctcct
     3301 aaagttaacc aagcaacagt ttgaaccgca taactcttag ctaaatattc atctcctaca
     3361 ataattagag actgaagaaa tttgtaaata ctgtaatatc catagactgt taatacgaca
     3421 gaaaaatcag aaaataaagt ggctaaccat ccgtagtatt tcattttagg gacataacta
     3481 aataattttt tgtatacttt cattttttcc tcccccgcat tcaatttctt ctcttattcg
     3541 tttacaactg ttaatgcttt ccgtaatagc gtaaattaga gatgtacaaa cagataaaag
     3601 gtaaactacg ttctattttt tgtatttttt taagactttt cttccaatca atacaccaat
     3661 catagataaa cttgctacaa ctaacactga aatgattccc catataccta ccgcatagtg
     3721 gatcattgca tccattgttg cagcagattg tcctttatct atccaatctt gacgaaaacg
     3781 atctgtaaag aaccatatag gaatccactg tccggcagca ctgcctaatt gcattagagc
     3841 ccaagacgca acttgaggaa ttgctcgatc atatccaaat acagatagaa taatatcagc
     3901 taagatcgcc atactaatcg aactgataaa ccaaggtaag tacgccccgc ccattaacgt
     3961 aaaaaataac aataaaacaa ttgtgtaaat agtaaaaatt ccttttctag ggtagttata
     4021 gctaatataa acaaatatga ttccagataa taatccaaaa atcccggggg atatgctgtg
     4081 accaaacgcc ccaccaatac tactaaccat tattgaaaa
//
LOCUS       DAZAID010000162         4067 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000162 DAZAID010000000
VERSION     DAZAID010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.166"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1157)
                     /locus_tag="ACG9BE_002815"
     CDS             complement(<1..1157)
                     /locus_tag="ACG9BE_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3033512.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
     gene            complement(1160..3409)
                     /locus_tag="ACG9BE_002816"
     CDS             complement(1160..3409)
                     /locus_tag="ACG9BE_002816"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN3033513.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3506..4009)
                     /locus_tag="ACG9BE_002817"
     CDS             complement(3506..4009)
                     /locus_tag="ACG9BE_002817"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3033514.1"
                     /translation="MENSIYLVTEPNTQLKDQDNLVSELSRQKQVYKFLELLLNSASY
                     LNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIK
                     EYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLG
                     LGIINGE"
BASE COUNT         1311 a          744 c          580 g         1432 t
ORIGIN      
        1 actctagtat tttttttatt tgtcgcaaaa aatgttacaa ataggatcac aataaatatt
       61 cctgcgaata caaaaaatgg agcagtcaaa tttgtacttc ttgttagaaa ttgtgctaat
      121 tgcatagcaa taggagctat aaaattacca atattacagc caataaaaat caatgaagta
      181 gcaaaattaa ttgtattctt tgtagtgact ttatctaagt tactaaagat aaatgggaag
      241 caccatgatc ctgttatact tgataaaaat aaacccgtta gcaggaacgt catattaccg
      301 tttgacacag caattaacaa atttgaaatt ataaagaata taattcccaa gtatagggtt
      361 ccttttccta tccacttatt tattggtcca aacataaatc ctgctagtat tcctaagata
      421 ggcattagag ctaaatagtt actagcatta aagtttaccc ctttaatttc agttgcaatt
      481 gatgcaaatc gtacagaaat agcaatacta ttcattacga gtaagattgc aaatagaact
      541 agggcaaaaa ccattggatt catcttttct tttacatgat tttcaggtat gtcactattt
      601 tcatctctaa tttcaggaac ttttagcgcg aataagagag ctacaggaaa cgcaattgca
      661 tagactaaaa acgaagccgt ccaattaatg ttcactaaaa ctccagcaag aaaaataagg
      721 attgtctgac ctaaagcttc cattgaattt ctaataccta atagcgttgc tctagtattg
      781 ccagagtata aagagctgat atatgtcaca gccaatgaat tatatactcc taaaccggca
      841 cctaatatta ctcgactaat aagaacggtt tggaaggtag ctgtaaatac tggaataatt
      901 cctcctatac cagctaataa taaacctaaa attattgtct ttttcattcc tattcttttg
      961 gcaaagtaac tagataataa gataaacaaa ataacagtta tacttggtgc agttcctaaa
     1021 agttcacttt gactttgtga tatccccaaa gactgtttca tttgtggaat aacaccatta
     1081 attgcttgag agcttgttaa cattagtgaa atacttaaga ttgctccttt ttgaagtaag
     1141 cttttttggt tattcatctc tacttttcct cctgtgttat aacccaaatc ttggaatcaa
     1201 aatcaaatgt ttctgggcgt cgattctgat catttatttg tccactagat gcatctgaac
     1261 agactagtcc tgctctcatc aattccattc cagatcgcaa actattttgt cctacattat
     1321 aaagtccgtc attatctaac ccttttaatt tcagtcggcg gtaaggagca tttacctcat
     1381 ttaatgtttt ataatgagca acaatagccg ttttcttatc atgactaaca accatccacg
     1441 cggtttgatt atcatcatcg aatggtgata atagtctata aaaagtacca ttatgaatta
     1501 tggttctata ttttttataa aaggcaattt gttcttttac aatcctcaaa tcgccttcac
     1561 ttaattcatt taagtcaagc tcatatccaa atgcaccaaa aaaagctaca tttcctctag
     1621 tttttatagg tgttatacgg tttgtttgat gatttggaac aattgataca tgagcaccaa
     1681 tagacgaaag aggatacagc atactagttc catattgaat ttttaacctt tcaattgcat
     1741 ctgaatcatc actagcccaa gcttgaggtg catagtgaag cattccagga tcaaagcgcc
     1801 caccacctga tgaacaagac tcaaatagta tgttaggata ttttgttata agtttttcat
     1861 ataagcgata tacgcctagt acatatctgt ggaatacttc tccttgttga tctgctgaca
     1921 agtgactgtc aaatacatca gtcaatggac gattcatatc ccacttaatg taatctaagt
     1981 ttgattcatc aatgatttta cacatttgat cgaaaatatt ttcaacaacc tcatctaacc
     2041 caaaatttaa tacatattgg ttcctaccat gattaggttt tcgattaggt gtagaaataa
     2101 tccaattcgg atgtgtgcga tataactcac tatccttatt caccatttca ggttcaaacc
     2161 ataaaccaaa tttcatacca aaactacgaa ttttttctga aaggctgccg attcctctag
     2221 gtagtctttt aggatttaca tgccaatctc ctaatcctgc acaatcgttt tctctttctc
     2281 caaaccagcc atcatctaat acaaataatt caataccaac atcaaatgct tttttagcaa
     2341 tatttaccaa cttttcttcg tcaaaatcaa aatatgttgc ttcccaatta ttaattaaga
     2401 caggacggtc tttttctcgc cacttaccac gagctaagcg ttttctaaat agttgatgaa
     2461 aagtttgact catggcattt aaacccttac ttgaataaac cagaatcgct tctggtgctg
     2521 taaatatttc tcctggattg agtttccaat taaatccaaa tggattgatg ccaacttgta
     2581 gtctagtaac atcccatgta tctacttctg cttgaattaa gaagtttcct gagtaaacta
     2641 gtgctgcacc aatagcttct ccttgatttt catcagtatt ttttcttttt aaagtaacaa
     2701 atgggttatg ctgatggctt gaaatacctc gagtagattc gattgattga attccttgtt
     2761 gtaaagtacg cgacttaata gttctttctc taccccaagc accagataat tgttgccatt
     2821 cataatttgc atcgggtaaa tccaatgata aactgctaat attctcgatt atttgaatat
     2881 cttttccttt attcataact cgcgcgctac gagtaataac aggataatct ctataaattg
     2941 tatataataa ttctacttga acatttgtaa ctgaatctaa aatataaact ttcaatgttt
     3001 tgctttcatc attattatca acataggtag caggtaaacc ttctagttta ggttttcctt
     3061 cttccacttc gtacccttga tagacaaaat ctgataagcg agagccattt ttctgtagta
     3121 atgaaatagc aggatgtcta aaatctgttg taccatattc aggaaattct tgttttaagt
     3181 gctctaacga atatagcaaa tcgttttcat ttctataggt tgtcataggc cgatgatgca
     3241 tctcaattag atgactatag tcattatctg gtaagttctc accataatat aaatgcaata
     3301 atttaccatc ttccgatatc ttaaaaatgt aacttatctc ctgattaaac aaatgaaatt
     3361 gttggttttc tgatcgataa ataatattca tttcattatc cttatacata agctctcctc
     3421 cgtttcttta ttgcattata aaaaacgctc acataaatag taagcgttta aagtaacgaa
     3481 tatttatcaa aatcaaatgt ttagtttatt caccattaat gattcctaga cctaatctac
     3541 gttttcgata tactcccggt gaaacaccat ttgctttctt aaaggcttta gaaaaagatt
     3601 gtactgttga gaatcctatc ttgtcactga tttgctggat tgaatattca gttgatgata
     3661 acagatcgtt tgcaatagaa attttgattt cactaatata ctcttttatt gatattccca
     3721 tatcattctt aaacaatcgt gataaataat tttcgtgtat cgatagactg acagcaatat
     3781 ctttaacaga taatttttga tacgcattct cacgtattat ttcaattgtt tgacgaacaa
     3841 aaggattcat ctttttagta attatccctt cgttcagata actagctgaa tttaagagga
     3901 gttctaaaaa tttataaact tgtttttgtc tggacaattc actgactaga ttgtcctgat
     3961 ctttcagctg agtgttaggt tctgttacta aatatataga attttccata tccaccctcc
     4021 tatgttagat ttggataatt tttgttttat tatgtttata ttattac
//
LOCUS       DAZAID010000163         4047 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000163 DAZAID010000000
VERSION     DAZAID010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.167"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..1424
                     /locus_tag="ACG9BE_002818"
     CDS             75..1424
                     /locus_tag="ACG9BE_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3033515.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1552..2142)
                     /locus_tag="ACG9BE_002819"
     CDS             complement(1552..2142)
                     /locus_tag="ACG9BE_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFN3033516.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2289..3641
                     /gene="brnQ"
                     /locus_tag="ACG9BE_002820"
     CDS             2289..3641
                     /gene="brnQ"
                     /locus_tag="ACG9BE_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFN3033517.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1164 a          742 c          786 g         1355 t
ORIGIN      
        1 atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac ttttttagtt
       61 aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa aatagacatg
      121 tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt tccggaaagt
      181 ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga tgcatctgtt
      241 ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac cattcttttc
      301 tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc tggacgtatt
      361 ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg tatgtacgct
      421 actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt cttcttcttt
      481 gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt gcattgatca
      541 aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc acccactata
      601 cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga ttctttccaa
      661 ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt gctgggatag
      721 aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg ccggaagcta
      781 tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt gtcattatgg
      841 caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa gtattttcgg
      901 aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt gcagcagctt
      961 ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta gcgcaagaag
     1021 gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg aatgctttga
     1081 cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg cctagtgaag
     1141 tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg agtatgcttg
     1201 tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat tcatttaaaa
     1261 tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt gtgtgtgtcg
     1321 tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg tggttcttgc
     1381 ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt agttgtgaca
     1441 gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt aaatttttgt
     1501 gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt tctatttccc
     1561 cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc aaataccaga
     1621 tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg caatcaagca
     1681 taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc tcacgacatg
     1741 ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag aaaaactcca
     1801 catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt tacgcccgac
     1861 ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat tcgaccagac
     1921 cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca cccagatcac
     1981 cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga cccataaaga
     2041 atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca aaacaatggc
     2101 taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc ctcctcagat
     2161 gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt atatagggaa
     2221 ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa accgtaaagg
     2281 agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc tccatgcttt
     2341 tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt caagaagcag
     2401 gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt cttcccttct
     2461 taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta gcaaaacgga
     2521 taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc ggaccgtttt
     2581 ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca tttatcgatc
     2641 ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg ctggcttggt
     2701 ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg aatcctgcat
     2761 ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta ggttctgtaa
     2821 atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg tttactcaag
     2881 gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt gtcacaacaa
     2941 tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc aaatctggtg
     3001 cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta ggtgctatga
     3061 gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag attgctgatc
     3121 attatttagg tacttatggc agtatcttat tagcatttat cgttatatta gcttgcttga
     3181 agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg tttccgcaaa
     3241 gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg gcaaacgtag
     3301 gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat ccattagcaa
     3361 tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg gctgtttatc
     3421 gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac gcttgcccag
     3481 aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa agctacttgc
     3541 cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt gttatcggac
     3601 tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg ttgtgaaaga
     3661 gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac atttttgcat
     3721 aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga ggtcgtgaaa
     3781 aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc acatttttgc
     3841 ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga gcgtctataa
     3901 actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt tgccgttttg
     3961 ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa tatagatttt
     4021 agacatatat atgatataaa aatagtt
//
LOCUS       DAZAID010000164         4011 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000164 DAZAID010000000
VERSION     DAZAID010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.168"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..617
                     /locus_tag="ACG9BE_002821"
     CDS             <1..617
                     /locus_tag="ACG9BE_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFN3033518.1"
                     /translation="NLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLG
                     MDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQ
                     FIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNH
                     TISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            634..1068
                     /locus_tag="ACG9BE_002822"
     CDS             634..1068
                     /locus_tag="ACG9BE_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033519.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1685..>4011
                     /gene="esp"
                     /locus_tag="ACG9BE_002823"
     CDS             1685..>4011
                     /gene="esp"
                     /locus_tag="ACG9BE_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFN3033520.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1466 a          561 c          766 g         1218 t
ORIGIN      
        1 gaaatttaga aaacatggtt tttaatctga gtaatggaat aattccaagt gttagtggag
       61 atacgattcg gtctgaaaaa aattattcta tcattgtttt tgaaaagtta gcacaaacgt
      121 ctataacatt aggaatggat ataatagaag cgtatcaaag tagagatgct ttgatacaag
      181 aaaacgaatt agctgtatcg ttacctgagg tcttaaaagt gagggattct gggattgttt
      241 attatactaa ggaaatcgga aaaactaaga tagagcatct ttctccctta atttcttctg
      301 tcgttcaatt catcggctta aatatctata aaagaattac tgtaaaggaa atagctaatt
      361 atttttctgt aagtgaaact aaattaagag aaagctttaa gaatgaaatg catattacga
      421 tttataatta tatctctaag cgaaaaattt ctacggctaa aataatgttg aaatcaaacc
      481 atactatcag tgaagtttct ttaggtttag gattttcaga ttcttcacac ttttcgagag
      541 tattcaaaaa gtacgcaggt gtatctccta agcaatatca attaggttta gtggacaatt
      601 tcaacaacat tagctgagag gttttatttt attatgagac tatatcattt acgtagtttg
      661 gaggagttga ggaaattgga ttttaacaaa aaattttttt gggaaatttc tgatttgagt
      721 tgtttcgtag aatctgagcg tctaagtaat ggtaataata gtgtgttaaa tgaatatgac
      781 aaagtagtaa ttaataaagt aaaaagttta tgtgaagaag ctagagaatc tatcagtcat
      841 attaatggta gcaattttaa acaaacatta tttaatctga ttaagctaga tgctaaaata
      901 tcaagttatt tattcttttt gatacatgat gaatttatgg attatcaaaa attggatcag
      961 ttaattgaaa gagaaagctg ggaagattat tacgtgggtc taactttttc agaaaagtta
     1021 aacaactaca agttaataga ttataagtat ttgagtgaaa gcaattaatt attcatgtag
     1081 agtactagac actttaaata tggacaattc aattataaag aaaaagaatc aataatttaa
     1141 tgaggctgtg acaaatgtca ttttttgttt cgctaaaaac gagtgaaaaa atgttgataa
     1201 ctccaatcat tcgtttaaga ttctaggaat cattcagaaa tgctgtgaaa ccgatacttt
     1261 ataattataa agaaggtgat ttcaaattcc gaagaaggaa tttgaagtca cctttttact
     1321 tatgtcacag tctcattcag taatgcggga taaggctcct tattccgtta tttgaatact
     1381 ctgaagttta atctaagaaa gggtaatatt ttgaaaacat gcttcatcgc atagaaatga
     1441 acttaatttc agaaagaata catacatatc tttttatatg ttttcagctg ctgaatggtt
     1501 aaaaagcaca aatatccgaa aaaaacatga agaaatgtac tttttttgtt gtaatgttca
     1561 tgaaattctc taagtaaatt agttaacata aggttagtaa gattcctatt ctgtgaataa
     1621 tacgtatcag atttatcttt tattcttact aggaaagtaa ttattaaggg agttgatttg
     1681 aataatggtt agcaagaata ataagagagt atttctagaa aaaacaaaga aacgagtatt
     1741 gaagtacagt atcaaaaaat taagtgttgg ggttgcatct gttttgattg gtgttggtct
     1801 tgtcttggga acaactgaac tagttaaagc acaagatgaa atttctccaa gcagcccact
     1861 tgaaacagcg ataagctccg tacaagtagg tgacaaagta gcttcgggga atacttttca
     1921 ggagaatcct ggttatacga aaaattataa cttttcggat ttacaattta gtcctcaaga
     1981 actaactgga gacacactaa aaggcaatac aattggattt gaagtttacg gaaaacataa
     2041 tattgcagca tcaactaaaa actgggaaat ccgtcttcaa ttagatgaac gattagctaa
     2101 atatgtagaa aaaattcagg tcgatccaaa gaaaggaatt ggatcttcta gacggacctt
     2161 tgtaagaatt aatgattcgc tgggtagacc tacaaatatt tggaaggtca attatattcg
     2221 tgcaagtgat ggattatttg ctggggcaga aacaaccgat acgcaaaccg ctcctaatgg
     2281 ggtaattacg tttgaaaaga gcctagatga aatttttaaa gagataggca tagacaatct
     2341 caagaccgat cgtttaatgt atcgaatcta cttggtaagt catcaagacg atgataaaat
     2401 tgtaccgggt attgatagta cgggctactt tttaacagat tcagatgatt tctataatag
     2461 cttagatgta tctgaaaata atcctgacca gtttaaacat ggatcagtaa gtgcaaaata
     2521 tgagaaacct aatactcaaa caaaagatgg ttcaggatcc acaggtgcta atggagctat
     2581 aatcttagat cataagttaa cgaaaaatta taatttctca tattcatctt ccgcaaaggg
     2641 cacgccttgg tatgctaatt acaaaattga tgaacgatta gttccatatg tagcgggtat
     2701 acagatgcac atggttcagg ctgacaaagt aacatatgat gtttcgtttg aatccggaac
     2761 aaaagtagct gatttagcaa tcgagcgacg ggaaggtcat gaaaattatg gcataggttc
     2821 aataactaat aatgatttaa ctaaacttat cgattttgct aatgcaagtc cacgtccagt
     2881 cgttattcga tatgttttac aattgactaa gccattagat gaaatcttag aagatatgaa
     2941 agcaacagca caagttgaag aaaataaacc atttggtgaa gatttcatct ttgattcttg
     3001 gttgtcggat acgaataaaa aattaatcca gaacacttat ggaacaggtt attattattt
     3061 gcaagatatt gatggtgatg gaaaccctga cgataaagaa gagagcggag acacgaatcc
     3121 atatatcggg aaacctgaat tagaagaagt atatgatgtt gacacaacag ttaaggggaa
     3181 agtattcatc cacgagttag cgggaacagg tcacaaagcc caacttgttg ataaagaagg
     3241 tactgtatta gcagaaaaaa ctatcgctcc aaatgaaaaa gatggggctc caatttcaga
     3301 tactgtagaa tttgaattta cgggtgtaga ttcaagtaaa ttaatcgcga aagatgaatt
     3361 aaaaatccaa atcgtttctc caggttttga taaaccagaa gaaggttcaa ccgttattaa
     3421 ggaatcacca aaagcggttg ataaacaaac cgtggtagtt ggatttaaac cagatgctaa
     3481 agaatcaatt cggaataata aaaacttacc tgaagatgca gagtattcat ggaaaacaga
     3541 gcctgatact tctaacgtta ctgatagtac gaaaggtatt gtaactgtta agatcggaaa
     3601 tcgaactttc gacgtggatg tagagtttgc tgtaaaagct tctcaagcta tggaaaatga
     3661 tgcaacatac gtacctataa caacaactcc agaaacgaca gttcaaagtg gaaaacctac
     3721 atttgataaa ccagatgttc ctttagctaa agatgccttt tcaatttcag atgtctataa
     3781 taagtacttc ggtaatgcaa gtgttgatgc aaacactggt attgttacat tcactccagc
     3841 taaaggtgta ggagaatcgg agccgattac tggaacaatt cctattaaaa ttgtttacca
     3901 agatggttct gtaggcacga ccgatttagc agtaactgta agtaaagata tttatgaaaa
     3961 tccaggagaa aacattcctg caggctacca caaagtaacc ttcaccgcag g
//
LOCUS       DAZAID010000165         3878 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000165 DAZAID010000000
VERSION     DAZAID010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.169"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(718..3867)
                     /locus_tag="ACG9BE_002824"
     CDS             complement(718..3867)
                     /locus_tag="ACG9BE_002824"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFN3033521.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          637 g         1362 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccct
//
LOCUS       DAZAID010000166         3779 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000166 DAZAID010000000
VERSION     DAZAID010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.171"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..308
                     /locus_tag="ACG9BE_002825"
     CDS             <1..308
                     /locus_tag="ACG9BE_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033522.1"
                     /translation="KFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGI
                     FLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(434..1111)
                     /gene="nth"
                     /locus_tag="ACG9BE_002826"
                     /pseudo
     CDS             complement(434..1111)
                     /gene="nth"
                     /locus_tag="ACG9BE_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(1118..1816)
                     /locus_tag="ACG9BE_002827"
     CDS             complement(1118..1816)
                     /locus_tag="ACG9BE_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFN3033523.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2084..3268
                     /locus_tag="ACG9BE_002828"
     CDS             2084..3268
                     /locus_tag="ACG9BE_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFN3033524.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3274..3717)
                     /locus_tag="ACG9BE_002829"
     CDS             complement(3274..3717)
                     /locus_tag="ACG9BE_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN3033525.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          975 a          748 c          628 g         1428 t
ORIGIN      
        1 gaaaatttat ggaaagaagc tctcatgcaa tgattctcac ttttttatgg ttgttgttca
       61 ccagaatcta tatttttctg atgagcctga aaccgcccaa atccattagt ttatgtttag
      121 ttataaatgg tattttttta ttatcactta tcatttttat tacaccttta gatagatacg
      181 tcacttattt atataatcct ttggaatact ggacttattt tataggtata ttggaaggca
      241 ttattttcta catcgggtat tttcccaata aaaataacaa cttttatttt tatcgcaata
      301 aattgtaata agttaccatc ctagctactg agtatgtaca tttacttttt catgcaaaaa
      361 agataaacga aacagctttt ctccataata acaagataat tcatacattt atttgcaatt
      421 aaagaaacca cgtctacagg cgtggtttct tttttagcgc cttttctttt agacgcatcc
      481 gatttttccc atcttgacat attgatagca aaggacatac ctcgcacttg ggatttcttg
      541 ccgtgcaatg atatcaacca aaaaaaatca gtgtatgatg tgtctttacc cataattctt
      601 gtggtacttt ccgcatcagt gtttcttcta cttccatgac cgtagcatct agtttgcaga
      661 tcctcaggcg ttttgagaca cgttccacgt gggtatctac tgcgattgct ggtatcccga
      721 atgcatcccc caatacgaca tttgctgtct ttctgcctac accaggaaga gacatcaatt
      781 cttctcttga cgttggcact tgcccatcga aacgttcaat cagctgctgt gcacacgctt
      841 tgatgttttt tgctttattt cgatacaaac caatcgtttt tattttaaga atgatttcat
      901 cgattgaagc ttctgctaac gcatcgggtg tcggaaaaga ggcaaacaaa tctggcgttg
      961 ctttattcac tgatacatct gtcgcctggg cacttaagat cactgcaatc aataactcaa
     1021 atggattatt gtgcttgagt tctccatgtg cttcgggaaa cattccatac atcgtttcta
     1081 gagcttccat tgttttttgt ttgcttaaca tattccgcta ctcctccaac caatttttca
     1141 gtgagacttt cggcaattct tcttgttgag ctgactgttc tacttctctt tgaagaagct
     1201 gctgttttct tcgtttttgg tcttcttcca cttgttgttt tgtacgtaga ttttttcttt
     1261 cccacgatag caaaatgcgg tcaacgtaat taaaactata agcctgattt aatacggctt
     1321 cccgcagtgc tagcttcaaa atttccggct gataatggtc ttcttctagc cattgtccga
     1381 tccgctggaa ctcgatcgaa gaaagcgggc gtccaaattc ttgttcaaat aattgataca
     1441 ctgactggat ttctttttct tgtgtttttt cttcgtattt tttttcttgt gtttttaaat
     1501 actcacctaa tagatcatag atggggtaaa gattatatcg atctgctttt ttaccgttga
     1561 caatcgtcgt ttctatcttg ataaatccat ttgttacgag acgatccagt atttgaaaaa
     1621 tctgatcttg tttgattccc atatccatag caatcatctg caaatcaggg aaagtatctc
     1681 cttcaagctg tgccatatgc aactgtagaa caaataaaaa ttcttcagat tgtagaccga
     1741 tcctatgata atttttaagt aaaagattag atatgactgt ttgtccagcg cttaagtaat
     1801 tttctaaatt caacatgatt tctcctccgc tcaatcacag tatacgtaaa aaaaacatgg
     1861 gaagcaagaa caggtttctt ttctatttac ttcactgatc ggtcagattt acaatgtcgt
     1921 ctacttgatt tttgtttgtt catgtttttg atgcgtatct tcacttttcg gaataaataa
     1981 attaagtaga tttcgctgtt ccttagaatg ttgacaagga aacaaaatta ctatacacta
     2041 tttttgtttc caagttaaca ttattgcata aaggagtttt tacatgtaca gtttcacaaa
     2101 taaacattct aaagtttttg gatacgtctc tgtcttattc actggattag gatttactat
     2161 cgtcagtcct gtacttccgt ttttaacgct gccttattct cactccatac atcagcaagc
     2221 tttctatatc actttgctca tgtctgttta tgctctagct gccttcttat ctgcccccat
     2281 tctcggtagc ttaagtgatc attttgggcg tcatccgatc cttattatca gtttattagg
     2341 atcaagtatc ggatatttgc tttttggttt aggaaattcg atatggatgt tgttccttgg
     2401 tcgtatcatt gaaggattga cggcaggaga aatctccacc ttatatgctt actttgccga
     2461 catcacggaa cctaatgaaa gaaccaaagt ttttggatgg atgggtgctt tagtcggtat
     2521 aggaacaacg ctgggtccta ttataggggg tctattagct gagttgggga attctgttcc
     2581 tatatttatt ggagcactct ttactttctt aaatgccgtt tatggctaca catttatgct
     2641 agaaagcctt ccaataaaaa aacgttcagt agatttaagt ttctcccatg taagaccttt
     2701 tcaccaatta caacaattat tcaagatttc atccgttatt cctttattga cagcaggttt
     2761 tgctgtttgg ttggcagctg gctctcttca atctatcttt tctcaatttt cgattgacgc
     2821 ctttcagtgg aaggctgggt tggttggttt aagcttctct ttgattggaa tcttggatac
     2881 tgtctcgcaa ttgttcatta tgcctcgatt gctcaaaaaa ttttcagaac agcagatcac
     2941 aagaataggg atgttcagtg aaatcctcgc ctatctattc atcacattat ctggaattct
     3001 tctactgccg attttgtttt tatttgggat tatctgttat ggtttcggtg attctatttt
     3061 tactcctgta ttcaatggcc aactatccaa ttctgtctca gataaccagc aaggtcttgt
     3121 aatgggcggt acccaaagta tacaatctct atcgcgcgtc atcggcccgc taatcgcagg
     3181 acagctttat gctgcagcac catttcttcc gacggtattc ggatttttgc ttatgatttt
     3241 tgcctattat cactacaata aaaaatagta gcttcatttg ctacttcgta taaattctct
     3301 gtatcgaccg ataaaacgaa atactgtgtc aaattcttct tttgacatat tttcaaatac
     3361 agtagcatct cgctgtaccc atttttgatg gagttgctcg tgtctattcc ttattttttg
     3421 tccgattggc gtcaattgaa aatagatttc ttttttattc tcttcgcttt gataagtgtt
     3481 tatgatccct ctagctatca gtttttttgt tagcttactg atggctcctc tagtcatgta
     3541 actagcattc gctaattttg tcacgttagg atcagggatt ttcccgatat gttcaatcac
     3601 ttctagttca tttagagtaa atcctgcaaa ttctttttcc atttctggtt tattacgatg
     3661 aataatctca ttagctaaat ccagatattc ttctattagc agttgtcttt tatccatttc
     3721 tacaccttct ttttctttca ttatattgtt tttttgtttc ctattcaaca aagataaaa
//
LOCUS       DAZAID010000167         3769 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000167 DAZAID010000000
VERSION     DAZAID010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.172"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACG9BE_002830"
     CDS             14..1141
                     /locus_tag="ACG9BE_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3033526.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="ACG9BE_002831"
     CDS             complement(1359..2501)
                     /locus_tag="ACG9BE_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3033527.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="ACG9BE_002832"
     CDS             complement(2506..2691)
                     /locus_tag="ACG9BE_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN3033528.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(3045..3290)
                     /locus_tag="ACG9BE_002833"
     CDS             complement(3045..3290)
                     /locus_tag="ACG9BE_002833"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3033529.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3284..3685)
                     /locus_tag="ACG9BE_002834"
     CDS             complement(3284..3685)
                     /locus_tag="ACG9BE_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN3033530.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT         1238 a          681 c          576 g         1274 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 taattttcaa tgaacaatag gttttctacc taaaagaatc tactaattcc ttaactatta
     3001 gaagattctt ttaaacagaa aaagtggaaa ggggattttt ttaattagtt aattttaaat
     3061 tttaagatcg cttctattaa tttaatttct agctgactta tcatatattc atctacatag
     3121 gtaacgaatt gacctacctc gttaaatcca tttgtcaaac ataacttagc catataagaa
     3181 tgataatgtt tgagtatcat cttgatcgaa attggatctc cttctagagc agataagata
     3241 atggatagtg gaactaatag gtactgccgc tttttagatt tatctaccat aggtcatacc
     3301 ccattttcaa ttgttcaact gccttctttc gttgatacca gataccactt gtgctcatat
     3361 tgagaatttt accgatttca gcatcattaa aacctgcaaa ataataaagt aggataatct
     3421 ttcttcttat ctccgataac tgatcaattg ctgctgataa cttttgatct gaaattaata
     3481 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gaatacctgc
     3541 cttcatttga atctgctaat ggttcctcat tatcctttat gttagcgttt cgttttcgaa
     3601 tagacttagc ttcgtttcgt aatacttttt tacagtaggc atcaaattgg tgttgtatag
     3661 atatgtcatt gtaatgttgt tgcatgataa tatgctcctt tttccccctt tccactaata
     3721 caacaatcag aaagcccctt tttttcgaaa aaactagaaa aaaaacata
//
LOCUS       DAZAID010000168         3718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000168 DAZAID010000000
VERSION     DAZAID010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.173"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACG9BE_002835"
     CDS             complement(<1..353)
                     /locus_tag="ACG9BE_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033531.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="ACG9BE_002836"
     CDS             complement(613..1161)
                     /locus_tag="ACG9BE_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033532.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="ACG9BE_002837"
     CDS             complement(1162..2019)
                     /locus_tag="ACG9BE_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFN3033533.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="ACG9BE_002838"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="ACG9BE_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="ACG9BE_002839"
     CDS             complement(2581..2910)
                     /locus_tag="ACG9BE_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN3033534.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="ACG9BE_002840"
     CDS             complement(2987..3259)
                     /locus_tag="ACG9BE_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN3033535.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="ACG9BE_002841"
     CDS             complement(3259..3522)
                     /locus_tag="ACG9BE_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN3033536.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1091 a          754 c          473 g         1400 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DAZAID010000169         3713 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000169 DAZAID010000000
VERSION     DAZAID010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.174"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..152
                     /locus_tag="ACG9BE_002842"
     CDS             69..152
                     /locus_tag="ACG9BE_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFN3033537.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            277..1014
                     /gene="erm(B)"
                     /locus_tag="ACG9BE_002843"
     CDS             277..1014
                     /gene="erm(B)"
                     /locus_tag="ACG9BE_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFN3033538.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1277..2140)
                     /locus_tag="ACG9BE_002844"
     CDS             complement(1277..2140)
                     /locus_tag="ACG9BE_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN3033539.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2142..2414)
                     /locus_tag="ACG9BE_002845"
     CDS             complement(2142..2414)
                     /locus_tag="ACG9BE_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN3033540.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2431..2640)
                     /locus_tag="ACG9BE_002846"
     CDS             complement(2431..2640)
                     /locus_tag="ACG9BE_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFN3033541.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2718..3614)
                     /locus_tag="ACG9BE_002847"
     CDS             complement(2718..3614)
                     /locus_tag="ACG9BE_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3033542.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1140 a          652 c          575 g         1346 t
ORIGIN      
        1 ttttgtataa taggaattga agttaaatta gatgctaaaa atttgtaatt aagaaggagg
       61 gattcgtcat gttggtattc caaatgcgta atgtagataa aacatctacc gttttgaaac
      121 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
      181 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttctgta
      241 tttatttata gatgtaatca cttcaggagt gattacatga acaaaaatat aaaatattct
      301 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
      361 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
      421 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
      481 gaaaaattaa aactgaacac tcgtgtcact ttaattcacc aagatattct acagtttcaa
      541 ttccctaaca aacagaggta taaaattgtt gggaatattc cttaccattt aagcacacaa
      601 attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa
      661 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
      721 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
      781 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
      841 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
      901 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat
      961 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
     1021 gagtcgcttt tgtaaatttg gaaagttaca cgttactaaa gggaatgtag ataaattatt
     1081 aggtataggg cacctctaat aactaccaat taattcacct caaaatgaaa ttagaaaaaa
     1141 acagcgaaat ttttaaatct atttcttatc gatacaaatt cctcgtaggc gctcgggacc
     1201 ccaacatgag gaaatgtttt tataaaaaaa gagtagttac cgttttttgg taactactct
     1261 tttccttttt taaggtttaa atacctggaa gtttaggtgt tttgggtatt ggtggtgtag
     1321 gtagctgcaa gctttccatt ttttgttgaa ttgctttaaa ttcaggggtt tcttggtgtt
     1381 gattttgaac cattttttgc tctattctct ccaaagtcgg ttgaatttct ttccctgata
     1441 ctttacgatt taattctctt tctaaggtct ctttcggact aatggaaggc gtttctaagc
     1501 ttgaatagag tttcactcct tctctattgt aaagccttat atcgctaaat aagcccgttt
     1561 tatgaagggt ttctaaattg gtcggtaagt ttttgacaac aatatcatgc gcttgttttg
     1621 gtgttgccct agctgtcatt ggatcatctg catacatggt ttcatatcgt tcaatcgttc
     1681 ctaagtatga ttcaattttc ggtactgcca tgacgtatat ttttgtttca taacctttag
     1741 attgaagcat tgtggctgtt tgaatgggaa cgtctgttgt tcgtcctgtt ccttcaatca
     1801 ctaaattata gccttgatcg cttaaacggc taataagtgc ttctgtcatg cgattcgaat
     1861 aaggggtaac gtgttttact acgtcttttt catagagttt cactaattcg tcaaaatttg
     1921 ggtgctgttg tttaaaggta tcattatcaa tgacaataac attcccttgt gtttcttcaa
     1981 aaattgctga tcgcaaactg gttttccctg accctggttg cccaccaagt aaaaaagcgg
     2041 ttggcgattc aaccgctagt ctatttttag ttagtcgttc aacattcttt tctaaacgat
     2101 cttcaaattg cttttcggtg aagtcagtaa tattcgccat gttaagccac tttctcttta
     2161 ttcaaaattt gttttgaata ttgattcatt gaccgccaat actcatgaat agcttgtaat
     2221 tcttcaaatg gttgttgaaa taaatcaact tcttgtgcca cttctaattg atttaaaaga
     2281 ttcacttcta gtaaacgagt atttttagta tctagttcat gattcaaaac ataatttaat
     2341 acccgattat aaacacttgc cgataactca ttaatgattt caatttcaaa tgttttttca
     2401 tacgtaactg ccatttcatt ttcactcctt ttaaagtttg tcaggtaaat atttccgtat
     2461 atattcttct aaggcttgat ccataacttc ttttacgttt ccgccattct ttgctgtttc
     2521 gatttttatg atatggtgca agtcggcacg aacacgaacc gtcttatccc ccattatatc
     2581 tttttttgcg ctgattggcg tatcatttcg ttttgctttc tgtgcgccta aatttcccac
     2641 gatcgctcac ttctttctat ttcttcttac tatttatttt atcatcacct atcacaaatc
     2701 acaagtgatt tgtgattcta ttctttttca ttttctaatt gaatgattcg ctcaatcatt
     2761 tcaaaaaata cgttctcata catagacaaa acttttttgt catagccttt gtgttctgta
     2821 atgccatttt tagaccaagt acttacttta ttacttcgct tgataatatt tcggaaaacc
     2881 aaattatcct ctttatgttc tttgtacagt tcttccaggt ttgattttat cgttgcgctg
     2941 tccgtatcaa ctaaataagg aacaaaacca atcatatcta gtccagggtt aaactgttct
     3001 tgcaaatcaa tcaaatagga aatatagttt tgaatgttgt ttgtactttc ttcttctgct
     3061 tgtaaaggga tcataacgta atcactcgcc acgattgcat tatttgtata aacgcttggc
     3121 gttggtacag tatcaataat aataagatca tagtcacttt ttaaaggtgc taaaagagta
     3181 gcaagcaatc tactttcatt ttcaaacgtc catgagcgag ttaattttgg cagtaacatc
     3241 aaatcaaacg tgccagggat caagtctaaa ttatcagtca aatgaataat agaagaagcc
     3301 aagtttccat ttttcaagcc ttcataaaaa ttgacacgtg gcaattctac ctcaaaagtt
     3361 tttgctaagt cttttgtcaa tgttgcctgt aagtccttat cgatcattaa aacttttaaa
     3421 ttcaatttgt ctgtcaagta agcaaacata gtcgataatt tggactttcc aacaccacct
     3481 ttaaagtaat tattcaagat aataattgct tcatttttat tgtttaaaat acgtcttaat
     3541 tcttcaagta tttttagttt ttccttctcc ataactacgc cccattcttt ttttgtatag
     3601 taatattgta tcattaagaa tcacaaatca caagtgattt gtgattaatc acttgtgatt
     3661 tgtgataagt gatttgtgat taatatataa aagccctctt taaagggctt tta
//
LOCUS       DAZAID010000170         3539 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000170 DAZAID010000000
VERSION     DAZAID010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.175"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(257..961)
                     /locus_tag="ACG9BE_002848"
     CDS             complement(257..961)
                     /locus_tag="ACG9BE_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033543.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1148..1393)
                     /locus_tag="ACG9BE_002849"
     CDS             complement(1148..1393)
                     /locus_tag="ACG9BE_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033544.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1410..2261)
                     /locus_tag="ACG9BE_002850"
     CDS             complement(1410..2261)
                     /locus_tag="ACG9BE_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN3033545.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2427..3431
                     /locus_tag="ACG9BE_002851"
     CDS             2427..3431
                     /locus_tag="ACG9BE_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFN3033546.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1104 a          602 c          688 g         1145 t
ORIGIN      
        1 tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa
       61 tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata
      121 ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt
      181 tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt
      241 atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag
      301 gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat
      361 tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa
      421 taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg
      481 aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt
      541 ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg
      601 tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca
      661 cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa
      721 ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat
      781 ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt
      841 gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc
      901 ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt tttttcttca
      961 tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag
     1021 gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc
     1081 cgttttttga aagaaaaata aaaaatggaa cggattatgt atatatacat aatccgttcc
     1141 attctttcta ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa taaatgactt
     1201 catgttcgtg ccagggtttg accattagcg aatgatcgaa ccgttttttc agttcttcga
     1261 tgatttgatc atgtgtccag tttcttgcag gaaaatgttg gattttctct gcatcgatca
     1321 aaaagacaaa atcggaatga aaagtctggg taacttcttc cgagagagaa ttcaagcgtt
     1381 cgattggatc cataaaatca cctttcgact taaaattggg tagtatctgg gtcttttgct
     1441 ttgccgacta caccatctaa ctgatcgatt tgtttcattt cttcttctgt taattcaaaa
     1501 tcaaataatt cagtattttc tttgattctg cttggtgtca ctgacttagg aagaggaagg
     1561 aattcgtgtt gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc atgtgcttca
     1621 gcgatttgtt tcatttcagg cacatcgaag attttaccag tgcctaatgg actatatgct
     1681 tcgaggatca tatcatgttt ttttgcatac tcaactacag ttgtctgtaa ttcgcctggt
     1741 gcaaggaaaa cttgattgac catcggcata atttttgctg tttttgctag tgtatctaga
     1801 tggtggggta agaaattgct cacaccgatt gctttgattt tacctgcttc atacagttct
     1861 tcaaatgctc gccatgtatc tgcatttgct tgttcccaat tatcacgaaa agcaacaggg
     1921 tttggccagt ggatcaaaaa cagatctaaa taatctgttt ggagcttctt caatgattct
     1981 tcaaaagaag acatcacaag ttcatagctg tggtttgcat tccataattt tgttgtcaag
     2041 aatatctctt ctctaggaat tccactttcc ttaatcgctt ggccgacgct ttcttcattc
     2101 ttgtatcctt gggcagtatc gatatggcga taacctgcag ccagcgcttc tttaacagag
     2161 gaaacagcga catccccatc aggtgtttgc catgtgccga atcctactac cgggatttcg
     2221 taaccatttg atagacgata agtgcttgtt aatgaattca tactttcagc tcctttttta
     2281 ttcgttcaac tataagtgta gcgcattttt cgaaaaaaag aatccatttt gtttctactc
     2341 ttgaaatttt tgtttaaaag aaaatgattt tgattctggt atttttgatt ttttttccgt
     2401 aaactaagac agaagggaat gagaaaatgg atatctattt aaaaaaagca attctgcata
     2461 ttattgacag agaaactggt tctccagtct tttcacaaaa agagctggat ctgacaaaag
     2521 aatatattcg tgattttctt caaaagaaga tccaaaaaat ctcttctgct caaacaaaaa
     2581 ctggacaact agcagaagaa agcatgatcg ttgaatcact taaaatcgct caagaaaatt
     2641 ttgtggaagc aagtgaagca atcgttcagc ggtggttcga tatctaccaa gaaagtgaag
     2701 aagcacctag tgcagatgta tttgttgtct tgtatgaatt agatacagtg atgtacttat
     2761 ctttattgaa agtcaattat cgtgaggctt acactcactt tgttgaagca gacgaagcag
     2821 ggatcgacaa ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca caaaaagcag
     2881 atgaagcatt cgctgtgaat ttgactgatt ggacctacga attaattgaa aaaaaatatg
     2941 agttttctgg caaaaaagag ctttattttt ctggaagagt gatcgaaagt gttcctgcac
     3001 cttctttaga agaaaatgta aaagtgatcc aaaaagtagc caagcatctt ggaaaaaaat
     3061 ttgagacaga agaatttgat atcatggctg atttgaaaga agctgtctat gatacgatag
     3121 aagaaaaagg tcggttggat catgagatga ttgcggaaaa agtcttcaag gaaaacatca
     3181 cggcaaaaat ggcttttaaa gaagaagtac aagagcaagg atttgtccca gaagcacctc
     3241 ctgtaaaaga agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa ttcaagctat
     3301 caaacgggat cgaactgatc gtaccagtag atgtatatcg caacccagat ttgattgaat
     3361 ttgtgaataa tccggatggt acgatatccg tgatgatcaa aaatgtggat gaagtattga
     3421 atcgtttata aaaatgacta gaatattagg tgttcaagta ccagtgatca gttaaatgaa
     3481 ttgtgtcaag agaaggatcg gaaaagacga atataacaaa aaaagagcgt gagacaaaa
//
LOCUS       DAZAID010000171         3433 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000171 DAZAID010000000
VERSION     DAZAID010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.176"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..2011
                     /locus_tag="ACG9BE_002852"
     CDS             83..2011
                     /locus_tag="ACG9BE_002852"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN3033547.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2149..2604
                     /locus_tag="ACG9BE_002853"
     CDS             2149..2604
                     /locus_tag="ACG9BE_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFN3033548.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            2684..2812
                     /locus_tag="ACG9BE_002854"
     CDS             2684..2812
                     /locus_tag="ACG9BE_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033549.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            3074..3343
                     /locus_tag="ACG9BE_002855"
     CDS             3074..3343
                     /locus_tag="ACG9BE_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033550.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
BASE COUNT         1051 a          611 c          675 g         1096 t
ORIGIN      
        1 ttatcgttct catttagctg ttattattat caattacatt tttattatgc tatccttgtt
       61 ttgacaagga ggtctaataa cgatgactaa tcagaaaaaa gcttttgcag caacaatcgg
      121 ctgcctttta tttatgatct tcggttttat cttcgaccga ttctctctta ccatcacccc
      181 acttttttat atcagtgcca ttatcatcgg tggatataaa caaacatcgg aaggcttgaa
      241 agaattgtgg aatgatcgga cactgaatgt ggatttattg atggctttag ctgctattgg
      301 tgcctgtttg atcggtaatt actttgaagg agcaatgttg acattcatct tttgtttaag
      361 cggtgcatta gaggaataca caactaataa aagtcaaaaa gaaattactg ctttgatgaa
      421 tcttcaacca caaaaagccc aattattaaa agaaaatgga caaatgcaag aagtagatgt
      481 ttctcaacta aaggtggata acttggtatt tgtcgctaaa ggagcagctg ttccgataga
      541 tggcttttta gaaagcacac aggcaacgat cgatgagtct gcattaagcg gtgaatctgt
      601 gcctgtagaa aaatatttag gcgatgagat gtatgcagga acgctgaatc aaggaaatcc
      661 catcacgatt cgagtaacaa aaacgagttc cgaaactgtc tttgctaaga ttatccaatt
      721 agtagaagaa gcgcagaata cgccaactca aacagcttca tttatcgaac gaatcgaaaa
      781 taattatgta aaattgattt tgcttgctgt tcctctaatg atccttctcc cccacttttt
      841 cctaggttgg agttgggatg aaagctttta tcgaggaatg gtattattag ttgtggcttc
      901 tccttgtgcg cttgtcgcat ctgctacacc agcttctcta gctgctcttt caaattctgc
      961 aaaaaatggg attttaatca aaggcggtat ccatttagaa aagctttctg aattaaaagc
     1021 gattgccttt gataaaacag gaacgctcac taaaggaaaa ccagttgtaa ctgacagctt
     1081 tttctttgat gaaaaggatt tggcacaaca acttcttgtt gctatggaac aaaaaacgac
     1141 gcatccactt gctcatgcga ttttgcaata tttcgattgc acaatacctg aagaaatcca
     1201 atacttacca atagaagaaa ttactggttt tggattacaa actacctatt taggtgcgca
     1261 atggaaagtt ggcaagcacg catatgatcc tgagacgatg ataatcagca aagagattgc
     1321 ggaaatgatc gaacgtttgg aaaatcaagg caaaaccgtg atttatcttt ctaaagatca
     1381 gcagttaatt gctgttttag gtctgctaga tattccaaaa gcaaataccc aacaagtaat
     1441 ttcctacttt aaatcccaaa acatccatac aagtatgata accggcgacc attctggcac
     1501 agcaaaggcc attgctgaac aagtagggat tgaacattac tatgcaagct gtactccgga
     1561 agaaaaaaca caactagtca aaaaagagaa tgaactttac caagtaaatg cgatggttgg
     1621 tgatggagtc aatgatgcgc ctgctttagc agccgcttct ttaggtgtcg ctatggggca
     1681 agggacagat gccgctatgg atatcgcaga catcgtctta atgaaaagcg acttaaataa
     1741 atttgtctac agtcatcggc tatctttaaa aatgcggcgg atcatcaaac aaaatattat
     1801 tttttcgatt cttgttattc tcttgttgat tgcttctaac ttcctgcaat ttttaaatct
     1861 acctcttgga gttgtaggac acgaaggaag cacaatattg gttattctga atggcttgcg
     1921 tttactaaga cctttacctg atctttctga ttcttcaaaa aaatgtaagt cttgtccact
     1981 atacaaagcg cggctgtctt ctgtcaaata agcaaagtgt ttttttatga ttttgtttat
     2041 tttatgaaaa aaacagctta tgaataggtc ttgaaggatg aattcctact tttgtaactt
     2101 tctaaaaaca tgttatgatt gtttcgaaga aagtgaggaa tatatttcat ggaaagttgg
     2161 ctttttttag gaattatttt acttgtagca attttagcta aaaatcaaag tctgatgata
     2221 gcggcagctt ttgttttgat tatgaagctc ataccagcct cccgttcctt tttcccttgg
     2281 cttcaaggca aaggaatcaa tattggggtg acgatcatca cagcggctat tctaatccct
     2341 atcgcaacag gagaaatcgg tctacgtgat ctattagaag cctttaaatc ccctattggc
     2401 tgggtagctg ttttttgcgg tgggctagtg gctgtcttat cttccaaggg tgtaggattg
     2461 attgcttctg atccacaaat cactgttgca cttgttttcg gcacgatttt aggtgttgtc
     2521 ttcttaagag gaatcgccgc cggaccggtc attgcttctg gtatcactta ctgtatctta
     2581 caagtatttg ctttaggaaa atagaacaaa ttttgtctat tttaggagat tcttaacacc
     2641 cccaatttac tgatccgtga tatactgaca acgaggtgat acaatgttcg aaaaattaaa
     2701 gtatttcata ggaacaatcg gctggtttct tctagcggta cttttgattc tgggcttagc
     2761 ttatctatta tttcctgatt tgatacaaaa tgtgtttgat actgcttttt gattgaccta
     2821 ttaaaacgat ataggggatg ctcctgcgat gcaggggctt tttttaatca atgttttgta
     2881 ataatttagg ccgtgccaat gagacttcct atagaattcg atgctctttt ttctttttgg
     2941 ctcttctgct cttttttgtt atactagatt acagtggaca aaagaaagaa tgatcgcatc
     3001 cacttcaacg aatatcaaaa tggaagaatt agtgcctgtt ttcaggttac tttgaaaaga
     3061 aaggaagacg cttgtggaaa cttttgaagg tcttttaatt gatacgtacg ttttacaaga
     3121 acagccaatg atgctgactg ggatcaactt gcagcttgag gaccgtgtga tcaatggcct
     3181 gcttaaagat cgctatttgg caaaatatat cttacgtttg cccctaaata aatatgtgct
     3241 ggaagtaagt ggcaaatgga accgtcggaa ccagttggtc ataaaaaaaa tgttgataaa
     3301 aaatatcgat gaatacatta aaataatcgg tacaccagaa tagcctttac tgatcaaagg
     3361 atactggtta taaaataact gcagtttctt aatagaaaaa caaagaacaa cagggaatcg
     3421 tatccctttt caa
//
LOCUS       DAZAID010000172         3389 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000172 DAZAID010000000
VERSION     DAZAID010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.177"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2303)
                     /locus_tag="ACG9BE_002856"
     CDS             complement(<1..2303)
                     /locus_tag="ACG9BE_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN3033551.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="ACG9BE_002857"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="ACG9BE_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          921 a          763 c          548 g         1157 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccata
//
LOCUS       DAZAID010000173         3256 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000173 DAZAID010000000
VERSION     DAZAID010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.178"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..692
                     /locus_tag="ACG9BE_002858"
     CDS             <1..692
                     /locus_tag="ACG9BE_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFN3033552.1"
                     /translation="TWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAEN
                     AWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIA
                     GNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQP
                     VVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRD
                     AGAVGGTLYAT"
     gene            682..1422
                     /locus_tag="ACG9BE_002859"
     CDS             682..1422
                     /locus_tag="ACG9BE_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN3033553.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1419..>3256
                     /locus_tag="ACG9BE_002860"
     CDS             1419..>3256
                     /locus_tag="ACG9BE_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN3033554.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1225 a          395 c          629 g         1007 t
ORIGIN      
        1 atacttggaa aaatattaaa gatacggcat acaatacgtg gatttctata aaaaatacca
       61 tgattaatac atggaataac attaaggact ctttctggaa tattgttact ggaattgtta
      121 attctgctga aaatgcatgg acaaatctaa aaaatggtgt ttcaaatgct attaatcggg
      181 taaaagaaat ctttaattcg ttaagggaag tcaatttatt tgaaattggc aagaacatca
      241 ttgatggact tataaatggt gtaaaagaaa agtggaatgc tttgaaagat actatcaaag
      301 gtattgcagg taatatcaaa aattggatta aaggagcttt agatattcat tctccatcaa
      361 gatggatgcg tgatatggtt ggtaaaaata ttgttcaagg tattatcgtt ggtattgata
      421 aagagcaaag taagttagat caaaccatga caaatttagt aaaaacaccg tctgttcaac
      481 ctgtagtaaa tggaataagt cacctaccaa ttacacaaac aaagcaaaat atatctgcta
      541 gtgaaacaaa agaaattcat ttacatttaa atgtttatgg agatttacct gattcaatgg
      601 ttcgaaagat tactacaaaa attaaaacag aattgacaag acaaatgaaa cgagatgctg
      661 gtgcagtagg aggaacatta tatgcaactt aagagaggac aattttttat taatcaacat
      721 tattcttctg aatttaatgt gtatattcaa aacaggcctg cttctgtttc agctagtcga
      781 gtaattgaat tgagagagcg agaaggaaat gattctatca ttattgataa aggctattac
      841 aaaaatgtga ctagaaagat tgagtgttac tataaagcac cttcaattga tatggtgcag
      901 gagtgggaag atcgaatcac tgaatggtta gatatgtcgt cttatagtga ttttattctt
      961 tactatgatg aacaatatat ttatcaagct gtagtaatag aaggacctga atttaaagga
     1021 acaagaaaaa caggaaatat tgtccctttt gaatttacag tgagtattcg accatttaag
     1081 gaaaattaca atggtagatt tactattcga caaacagaaa cttttgaaat ttataaccca
     1141 gagaagtatt cttcaaaacc gcttattaaa ttgagtggtt ctggagatgc ttctttttat
     1201 attaataagg ataaatatga tttgaaatcg ttggacagag aattatttat agattctaaa
     1261 ttagaagagg cttatcgaaa attagatggt aatttagaac atcaggatca ggtcacctta
     1321 tttttagatt ttccattttt atatcctgga aaaaatgaaa ttaaatggac gaaaaatatt
     1381 cattcatttg aaataatgcc taggtggtgg agaaaagtat gaaaccaaga atatatagtc
     1441 ctactgaaac agatttctca acgaatggct taggaatttt aaaggattgt acaagatgtg
     1501 aaatatatga agtagctaat ggaaaatatg aattagagtt ggattatcct ttaggaacta
     1561 gatttgatga atattttgaa aatgactatc aaataaaagc aaagccaaac gatcaagaag
     1621 agtatcatat cttttttatt gatgataaag atatagatac ttttttaaat acagtaacta
     1681 tttatgctca gagtcgtaca aatcgacttg gaagacgggt agtcactctt gtggaagtgg
     1741 actctaaaac tggtcaagaa gcgatatcaa ttattgaaac taaaatggat aaaaaatctg
     1801 acatacgact ttattctgat attacggctg tttctagcac aatctttgaa gcgagaaatg
     1861 ttttaaattg tattgctggt gaacaaggat cattgcttca atattggggc ggagaaatta
     1921 aacgtgaacc atttaagctt tctttgttaa agcgaagagg tcgcaataat gttggaacga
     1981 ttcggtatgg aaaagatatg tctggtttga aggtcaaatt agattggaca ggtataaaaa
     2041 caagaattat tccatatgct gatcctcaaa gtgaagtagg tacgactaat cgaatttatg
     2101 gttcgccagt agatagtgcc tatattaata actatcctga tgtctataca gaacatgttc
     2161 agtttacaga agaacaagga gtaaaagaca ttaagagctt aaataaaata gctaaaaatt
     2221 actttaaaac gatcaatcct ggctgtgata agcctaaagt ttctattacg gttgaatttg
     2281 ataagttgac tgatagtgaa gaagcgaaag aatttgcgaa gattagaaat tatggtctgt
     2341 ttgatacatt taaaatatat cataaaaaat ataatattta tcttgaatca aaggttagtg
     2401 gactacaata cgattcttta tctgaaaaaa ctttgaaatt agaagcagga gacattcaag
     2461 ttgcttttta tcagcaacaa gctgtaacta ttcaggataa attaaaagat tatgcaacga
     2521 ataattatat gagtgatttt aatgattatg tttcttcaat gattacaggt caaggcagtg
     2581 caggaggata tgtagtttta tggccaaaag aaaaaccttc caacatcttt attatggata
     2641 atccagattt agaaaaggca agagaagtac taagaatgaa taaaaatggg attgcttttt
     2701 ctaagaaagg atggcggggg ccttttaatt cagcttggac attagatagt atatttaatg
     2761 ccaattttat acaaactgga ttaatcaaag cagatatctt tcaaaattct tttaacaaga
     2821 ctggagatgt attgaaatta gttaatggac tactccaaat ttggaacaac aaaaagaaaa
     2881 taatggagtt aactaaaaaa ggaatggaat tttggaattc taagggttca attggaacga
     2941 ttggaacaac tgattctgtt ggtaatcctt ttcctggggc ttctactcca acccctattg
     3001 aagataattc tttagttatt cgtacaaatg gagacggcaa atatattttg atttctccta
     3061 aagtaggaaa aggattagtt ttattaggaa atggtaaagc aatttatttt ggagacttag
     3121 atgtacaagg aaaacttaca gttaaaggaa aagaaataat tcctggacaa ggtggtggtc
     3181 catcgggagg tggagaatct actggtgggt atcctagtga attaaaaaca gatgcagaaa
     3241 agagagcttg gagaat
//
LOCUS       DAZAID010000174         3230 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000174 DAZAID010000000
VERSION     DAZAID010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.179"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..2556)
                     /locus_tag="ACG9BE_002861"
     CDS             complement(64..2556)
                     /locus_tag="ACG9BE_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFN3033555.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2563..3030)
                     /locus_tag="ACG9BE_002862"
     CDS             complement(2563..3030)
                     /locus_tag="ACG9BE_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFN3033556.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          856 a          718 c          532 g         1124 t
ORIGIN      
        1 tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac acaggccaag
       61 taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg tgatcttacc
      121 ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa gcaaagcctc
      181 gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg caccatattc
      241 aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt tcacttgaat
      301 atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga ttgtatggat
      361 ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca agaattcagg
      421 tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt gatctttagt
      481 aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg ctccgatatt
      541 tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag aatctgttaa
      601 atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg ctttctccac
      661 ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca actgtccacc
      721 ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac tgtatttttc
      781 cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca tgacttctga
      841 aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca tgaatgaacc
      901 aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac gtgaaactgc
      961 tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca gttctaacaa
     1021 gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact ccgatacaac
     1081 tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt ttgcttcaat
     1141 tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta attgagcagc
     1201 attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt catcgatttc
     1261 tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga ctttcgctgc
     1321 tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga tataacgaac
     1381 agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat ggtgctcttc
     1441 atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg gctcatctac
     1501 ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt tttgatattc
     1561 atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg gtttcaagat
     1621 attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat gcaattcatc
     1681 aatgaataag attacttgac catcattata gatttcgtcg atgacctttt tcattcgatc
     1741 ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa gcatcatcag
     1801 gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag ctaacccttc
     1861 agcaatggct gttttgccta caccaggttc acccaccagt acagggttat tctttgtccg
     1921 tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa ctgggtccag
     1981 cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg taggtgttcc
     2041 ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca ttccttttcc
     2101 agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt ttgaaaggct
     2161 gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta ataagctcat
     2221 caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc tgctagctaa
     2281 agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag ggtaagaacg
     2341 gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt cattcaaatc
     2401 catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg ctagtaaaac
     2461 atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg caatttctaa
     2521 tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc catcttcata
     2581 acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt catattcatt
     2641 gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt tttttgtaat
     2701 tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac tgattgtatc
     2761 atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc ttacttttgc
     2821 aattcgtata taaccaccac caccacgttt gctttctact agataaccac gttggatggt
     2881 aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga ggtctgccat
     2941 ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta tatatgattc
     3001 aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact aatattgacc
     3061 ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta aataaatcct
     3121 ttagaatcaa caaaaaaaaa tacggaatat actattccgt atcgacgcgc ccaagaggag
     3181 tcgaacccct aaccttttga tccgtagtca aacactctat ccagttgagc
//
LOCUS       DAZAID010000175         3221 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000175 DAZAID010000000
VERSION     DAZAID010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.180"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(273..641)
                     /locus_tag="ACG9BE_002863"
     CDS             complement(273..641)
                     /locus_tag="ACG9BE_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFN3033557.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            791..1273
                     /locus_tag="ACG9BE_002864"
     CDS             791..1273
                     /locus_tag="ACG9BE_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFN3033558.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1310..2320
                     /locus_tag="ACG9BE_002865"
     CDS             1310..2320
                     /locus_tag="ACG9BE_002865"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFN3033559.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2435..3148)
                     /locus_tag="ACG9BE_002866"
     CDS             complement(2435..3148)
                     /locus_tag="ACG9BE_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033560.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1088 a          519 c          624 g          990 t
ORIGIN      
        1 tattatgaaa gatcactgat attttctcgt attttttcct tattcaaaag ttttgcagga
       61 aaattgctga aaaaataatt ttactaagtg gaaaattcaa agccattcaa tcagtgattt
      121 ttttcagcac aagaaagact ccttataaaa atagcagata ctttgtcaat taagtgttta
      181 accagggatg ataaaaagga taggattgaa caaaacctgt ataagctgat gaatagtttc
      241 ctcgccagta aaaggtttat tcgtataaag ctttatcgcc aaaatttatc atgacctttt
      301 ttatcttcaa tattaaatga aatcaattgt tcaatcaatt cccagttgat tacacttttc
      361 caaggaactt tgatgacgtt atccgtatat acgtagcctg cttcttggat atttttttca
      421 agggcacgaa tcgctgcttt ttccggtgcg atagaaaaat gatttttagc aaaactaaat
      481 gcaataataa aggtaccatg gtcagtaaac ataggttggt tccattttac agttgtgtct
      541 agctggggat acgtttctgt tatcttagca aaaaggtctt tgatcttttg ttgatgttct
      601 ttttctttga tctgattgat gatcggctga aatttttcca ttctaaaacg cctccattaa
      661 gtggtttgat ttattttacc agaaaaacaa tagttatgta taaaaaacga acaaaacaca
      721 agtaatgttg taaccgctat atttatatga taagatgtct caaataataa aatagaaaag
      781 agacataaaa atgattgact tggcaaagaa attcttacac gaaaatatat attttgagtt
      841 gtatcatcat cgagccattt atacaaatga agatgctttg gtaataaaga aagaacaagg
      901 atttacaggt acagagacca aaagcttatt cttaaaagat aaaaaagata ataactacgt
      961 atatctgact tttactacga aacaaacgga tttcaaagca ttagggaaga tcgtcggtaa
     1021 aaggctttct attgtttcat cagaacgcat ggaagaacaa acgggacaaa agcagggcgc
     1081 tgtttctccg tttggttatg aaaaacagat tccgattatt attgatgaag agctcttatc
     1141 aaatgaaaaa cttgtttttg caccaggtag accagatcaa acgatggtca tccaaacggt
     1201 tgacttaaac aaaatcatcc agctacttga gatgccgata tttttgatgc cggcaaacga
     1261 aaaaaacaat taaccaagta aaatagtgca tacttggagg tgaagcaaca tgataagtta
     1321 tccattatta gaaagaaatg caacgattgg ggttactgca ccatcctcag gcgtatcaga
     1381 ggaacaccat tcgttactcg aatcagcttt aaagagaatg gaagaaaaat ctttccaagt
     1441 agttactgga aagacgcttt ggacgcaaga taaggaaaaa tcaggtccag caagaaaacg
     1501 agcagatgaa ttcaatcaaa tgatgcaaga tgaaaatatc caaatgatct ttccgccttg
     1561 gggaggagaa ttattgattg agattctcga ttttgtcgat tttgatgctc tacaaccaaa
     1621 atggatagta ggttattcag atattagtac gttgctactt gctgtcacat taaaaacagg
     1681 gatagccact gcacatggaa ccaaccttat tgatttaaga ggagagtact tggatgaaac
     1741 gacagcaatg tgggaaagtg tactgaaaac aagagaagga gaatcgattc tacagcaacc
     1801 ttctgaaaag tatcaaaaag agtggcccca tgagcattca tcatcttctg ttttccatct
     1861 cactgaacca acaaattggc aatcagtcag cggagatgca gtaaggatga agggacgatt
     1921 attaggtggc tgtattgata cgatcagtca tttaatcggt accccttatg gagatgtcca
     1981 atcttttaaa agaaagtata atgatggaaa gccgatcttg tggtttctag aaaattgtga
     2041 gctatcgtca gttgatttaa gaagatcact tgttcagatg aaactggcag gatggtttga
     2101 tggcagctcg gggatcatgt tcggtagaag tgcagcagag gatttgttag attataaaat
     2161 gaaggatgta tatgaagatc tgtcaaagga attaaaacta ccgatcttgt atgatattga
     2221 ttgtggtcat gtaccgccac aactgacatt gatcaatggt gcctatgcag aagtagcggt
     2281 agaagataga aaagcgactg ttttgcaaac gtttatttaa aaaatttttt cctgccttgg
     2341 agtttatcaa agaccaacaa atctaaatag aaaagaaaat gaaagaagct gacagtattt
     2401 tgccagcttc tttttgtatg atctattaga aattttaatt atccataact atcacattga
     2461 tcagctgttt cacatcattt ggattacctg taggaatagc tactacagat gcattatcaa
     2521 taatttcttg agttgtttct ctaatgaacg ctggataaga tgtttgatct gcaggcattt
     2581 gccaatgtga aggaactgga taactagtga ttgtcccgtc atggttactt gaatacacta
     2641 aaactccctg gtaactgtat ttcccagtga gcataaccac atctttcgga tagactgcac
     2701 tccctgcatc ataaggggcg ataggtgttc ctgcaggata atgaataaca tgtagttcga
     2761 aatcacccga tgtatctcta aaagtattac ctctaattgc tagccaaaca cgtgcgtact
     2821 caatttgtag atcagaatat cctgctaacg gatctattga ctgagaagac tgtgaggaag
     2881 ttgtgtctat tgttgaagaa gaaatatttg tctcaactgt actactagat gtagaagaag
     2941 tattgggctc tgagacttta ctgcttgttg aagatgtatt cgtttcagta gaagctgatt
     3001 tctgagtact tgatttttca gctgaactgg tttgtttgat tgaacttttg tctgtggtcg
     3061 cagatggatc actattttct ttactaggag acgtacatcc tacaaggaat aaagagacca
     3121 acaataaaat aaaacctaac tttttcatat aaaatacctc attatttttt taataccagt
     3181 atagcataat cctgaatttt tgaatccgta aaaccttgtc t
//
LOCUS       DAZAID010000176         3065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000176 DAZAID010000000
VERSION     DAZAID010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.181"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..596
                     /locus_tag="ACG9BE_002867"
     CDS             168..596
                     /locus_tag="ACG9BE_002867"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFN3033561.1"
                     /translation="MIFGKKHSLVEVPEFGSEPYLIEIGDNFRCSFGVKFITHDGGVW
                     VLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANTLVNKDIPNNS
                     VVAGSSCKVISTIEEHYEKNKNEFIKYRTSMILKQKNFFY"
     gene            684..1850
                     /locus_tag="ACG9BE_002868"
     CDS             684..1850
                     /locus_tag="ACG9BE_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFN3033562.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
BASE COUNT         1089 a          384 c          590 g         1002 t
ORIGIN      
        1 tgctattttc gtttttacgt ataaacaatt aattataacg tatctaaaaa aatttttcag
       61 aataaaaatg gctaaaaggt gagggtatac gaagtaagaa tagaatcttt cgaagaataa
      121 agagaaaact atttggaaaa aatctgcgag aatgtattaa tgaaggtttg atattcggaa
      181 aaaaacatag tttagtagag gttcctgaat ttgggagcga gccatattta attgaaatag
      241 gggataattt cagatgttca tttggtgtta aatttataac acacgatggt ggagtctggg
      301 tcttaaggaa gatgagttct gtgagcgagt caacttcaaa aataggtagg atcaaattgg
      361 gaaataatat ttttattggt atttactcta ttgttttccc aggagtttca attggcgata
      421 atgtaattat tagagcgaac actttagtaa ataaagatat cccaaataat agtgttgtag
      481 cgggtagttc atgtaaagtg atttcaacta tagaggagca ttatgagaaa aataaaaatg
      541 aatttattaa atatcgaacc tcgatgattt tgaagcaaaa aaattttttc tattaaataa
      601 tattgattga ataaggaata tgttcaaaag gataacagct aaaatactat aaaatgaaat
      661 ttgattggag cgtatggaag aatatgaaaa tttcagtttt cggattaggc tatgttggtc
      721 tagcaaattc actattatta gcacaaaaag agcaggtcaa agcttatgat atcgtggaag
      781 aaaaaatcac gatgttacaa aatggcatct caccaataga agataaagaa gttcatgaat
      841 ttttaaaacg ggatgactta aatgtcgaat ttacgtcaaa ttttactgat gcagtaaact
      901 tcggtgatta cttgattatt gcaactccaa cagattatga cgaaaagaaa aactatttta
      961 atacatcaac agtagaagat gtaattgaga aggctttagc aattcgtcca gattcaacat
     1021 tcattattaa gtcgacagtt ccagttggct atgcaaatga aattaaagcg aaatttggta
     1081 cagaaaacat cattttttca cctgaatttt tgcgtgaagg aaaagctttg tacgacaact
     1141 tatatccatc aagaattata gttggagaat gttctgctcg tggtcgcgag attgcaacta
     1201 tgtttcaaag aaacgcattt aacaaagatg catcagttct tttgactcac tcaactgaag
     1261 cagaagctat taaattattc tcgaatacat atttggcatt gcgagtggcg tatttcaatg
     1321 aattagatac gtatgcagaa tttcgtgggc ttgatagtaa gcaaattatt gaaggtgtcg
     1381 gtttggatcc acggatcggt tcgcattata ataatccttc ttttggctat ggtggatact
     1441 gtttacccaa agacacaaaa caattaagag ccaactatga aaaagtacca agcaatatca
     1501 tcagtgcaat tgttgatgca aatacaacaa ggaaagattt tattgctgga cagattttgg
     1561 cgaaaaaacc aaatgttgtt ggaatctatc gtttaacgat gaaggccggt tcagataatt
     1621 ttcgatattc gtcgatccaa ggaattatga aacggattaa agccaaagga attgaagtgg
     1681 ttgtttacga gcctacattg gatgaagatg aattttatca ttcacaagta ttgaaagata
     1741 ttaatgaatt taaaaatatt tctgatgtga tcgtatctaa tcggtttgaa gatattttaa
     1801 aggatgttga agaaaaagtt tataccagag atatctttgg tgataactaa ataataatga
     1861 acgggtacat ggaggaaaat attttagatg gtgactcgaa ataaatgttt gttctcattg
     1921 catctgtatc tataccgata agtttaaaaa ttccgattag gaaaatgaag ttggctatcc
     1981 ttgcgaggaa tttattgctg gattatttta tgaactaaag caagacatca tcagaaaaaa
     2041 atccaaaatt tgacaatggg attgatcgtt taccagctgt aagcgaagta tctgacacga
     2101 aaatggaaaa tgcaattact gttattacca atagcttttt ttcatcgatc aagtatttga
     2161 aaacattatt tgtttgccaa gctgatcaat gttctttcag acttggaaga acattgatca
     2221 gctcacaagt aatgattgaa gtttaggtta tgggataaag gataagtgaa ataaaagtcg
     2281 tgtctgcatc tttgttagta ccagtaaatt tttctactac ggcgctagtc acgacagtcc
     2341 aggcatctga aattgaagat gcaaatagag tttctgctag tttaggtatt gatgttactg
     2401 tattagcatt aatcttagag ccagtagcat ccaataggtg tagctatagt tctaatagta
     2461 taaaacaatg cagaggatat gattgcttta tgtctcggtg tgtcaggtca aaatagagtt
     2521 ccatatgggg cgatttctca atgaataatc ttatagatat aatcttgttt gctatttcgt
     2581 tatcactttt acttttcttc gattatttca aaaataaaaa tccaaaatat tttaagttct
     2641 tactatttat cattactgta ctagctgttt tatcgatgac agattttatt taacttatct
     2701 caaaaagtag ggaaccatat catttttcag taacatgcgt attatttgta taaataatac
     2761 aaaatgaaaa attatgatta ttctattgtc tttttagaaa gcgttttctg tgagctttta
     2821 atctaaaagg agggatataa aataaagaaa acctatttta tgctattttt ttgttgtttc
     2881 tgtgtaggaa tcctgtggta tataggtggt aatgaaaaga agattcatgg agttgtgaca
     2941 aatatttctg cggatgagtt cgtatttgaa gatgaaaaac agaacgaata tagtattcaa
     3001 aaaagaagct ctttgtcaaa tgatgttggt aaagagaaat cttagaataa tttggacaga
     3061 agagg
//
LOCUS       DAZAID010000177         2747 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000177 DAZAID010000000
VERSION     DAZAID010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.183"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..818)
                     /locus_tag="ACG9BE_002869"
     CDS             complement(<1..818)
                     /locus_tag="ACG9BE_002869"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFN3033563.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHVVMFALFFSANLFLVHKLSKMNIYEKKVISLSSYIFSINMLA"
     gene            complement(790..1686)
                     /locus_tag="ACG9BE_002870"
     CDS             complement(790..1686)
                     /locus_tag="ACG9BE_002870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN3033564.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
     gene            complement(1676..>2747)
                     /locus_tag="ACG9BE_002871"
     CDS             complement(1676..>2747)
                     /locus_tag="ACG9BE_002871"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033565.1"
                     /translation="SIEYFNVVSDYSHIRKQFLSSKERHMIKNRHVIKTIGYKKNISV
                     MRILSHIWFSIKTLLFIRKEKPEIIYCRIPPNTLVFFIAKFSRCNRVIFDVYDLWPES
                     MPGIGNKYKFFTDLWKKLRDQYLSNSSLVITECDYFKKFIKISGESKNMTIYPCSFNV
                     DDQNDYGEKIVNKSLESTLNLAYLGTINNIVDLEAINELLKTCKRKFGNKINLYFIGK
                     GESKTKLADICEKNHIEFVDCGVIYDNAEKKVILDKCHFGLNLFKSNTIIGLSLKSIE
                     YFRFGLPIISNLSGDTKEIVKRYNVGINYNSTVDENEMLKFYQCNKNINENVRKIYAT
                     IFSEQVLKMKWNEVYKVMNDEK"
BASE COUNT          948 a          453 c          332 g         1014 t
ORIGIN      
        1 gctaacatat tgatagaaaa tatatatgag cttaagctga taactttttt ttcgtaaata
       61 ttcatttttg ataatttatg aactaaaaaa agatttgctg aaaaaaagag agcaaacatc
      121 actacgtgag aaatcgatgt actgtttcta tcaaaatatg catcatactt cgtatttata
      181 aatattgaac tgatataact ttgtccaata gagaatagga gcaagctaag gattgtaaca
      241 tatccagtaa tctttttatt aatttttaca ataaaaaaag ggagataaaa atatgcaaga
      301 ttatggaaca gtccagccac taagataaaa aaaatatact taaatatagc tttgctgcct
      361 cgcaatttag ttagatgata aaaagctagt aatatgaata tcaatgcgct aaacatacgt
      421 acttgtattg cttgtgaagt aaaaccaaaa ataaaaaaca agaacaaaac taaaaaaaca
      481 ttagttgtga ataatctgat gacatacaat aaagctgaga gtaagacagt agaaataatc
      541 aataaaaaaa tctgatactg actggaaata aaggtacata cgtacataac agctctgaat
      601 cccggttcaa tactatctga actaaagtcc ataccaaatt gtgctatccc attatacaag
      661 acagaatatg cataataatc agcgttttgt gtgttaaatg ccatgatgat ccacattatc
      721 aaaagtagca ccccagacaa aatcttgttc gatgtactcc attttttttg tgaaacactc
      781 ttttcttgat taatctccaa tgacgcctct gtatccatct cgaacccccc ttatagccgc
      841 tctaaatcta ctgagcttat cagtctcaaa agctattgca ataaaaaatt tcaataatct
      901 tttattcata aggcgggaaa attccttatc acttttatac attcttttca ccaatgttga
      961 atttcttgca ataaaatact ttcgtgttga attatgattg tctattttaa ttttttttga
     1021 tcctattttt aaattttctc catctccaac gtgatgataa aaatagactt gatttgttac
     1081 ataaatgtta tagccattat ttgcaaccct tttacaaaat tcaacatcaa tctgatcaat
     1141 aaaaaattca tccttcatat ttccaacatc gtcaattaat tcacttttga aaaacattcc
     1201 agaggaattt gcaaatgcta ttttcatgga accactagtt gaagcaaagt gtttttttac
     1261 gtatctccct ctctcatttt tgcaggtaaa agagacagat tcatcattgc tattctcaaa
     1321 gtaattggga acaataatcc caatgtcctt atctttcgct tcaatattgg taaactcttc
     1381 taataatttt aaagaaagat tcttttgtat gatggtatct tgatctagta aaaggatata
     1441 atcacatttc tcttttttgg caatttcaat acctatgttt tgagctgtcg caagcccaat
     1501 gttctcattc atttttttta aaattaagtt ttccatcatt tcaaattgaa aagagttaat
     1561 attatctgag ccgttatcca cgataataag tttgttctgt ttctcttgta ttaacgtatc
     1621 cactaataac tttatgttat tatctggatt gtaggtaaca ataattatcc caattttatt
     1681 tttcatcgtt catcaccttg tacacttcat tccatttcat tttcaatact tgctcagaga
     1741 atatagtggc gtaaattttt ctaacatttt catttatgtt tttattacac tgataaaatt
     1801 tcaacatctc attttcatca acggtactat tataattgat tccgacatta tatcttttaa
     1861 ctatttcttt cgtatctcct gataaatttg aaattatagg taatccaaac ctaaaatact
     1921 caatcgattt cagacttaaa ccaattatgg tatttgactt aaataaattt agtccaaaat
     1981 gacatttatc gagtataact tttttttctg cattgtcata tataacaccg cagtcaacaa
     2041 attcaatatg atttttttca caaatatcag ctaatttagt tttgctttca ccttttccaa
     2101 taaagtaaag atttattttg tttccaaatt ttcttttgca tgttttcaat aattcattaa
     2161 tcgcttctaa atctacgata ttattaatcg tacctaaata agcaaggttc aacgtagact
     2221 ctaagctttt attgactatt ttctctccat aatcattttg gtcatctaca ttgaaagaac
     2281 atggataaat ggtcatattt tttgattctc cggaaatttt tatgaatttt ttgaaataat
     2341 cgcattctgt tataactaga ctagaattac tgagatattg atctcgtagc tttttccata
     2401 aatcagtaaa aaatttatat ttgttcccta ttcccggcat tgactctggc cataaatcat
     2461 acacatcaaa aataactcga ttgcatctac taaacttagc aataaaaaaa actaaggtat
     2521 taggtggtat tctacaataa ataatttcag gcttttcttt tcgaataaat aataatgttt
     2581 ttattgaaaa ccatatatga gataaaattc gcattacgga tatatttttt ttatatccga
     2641 tcgtttttat cacatgacga tttttaatca tatgcctttc tttactggat agaaattgtt
     2701 ttcttatatg tgagtaatca gatacaacat taaaatattc gatactg
//
LOCUS       DAZAID010000178         2674 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000178 DAZAID010000000
VERSION     DAZAID010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.184"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            699..785
                     /locus_tag="ACG9BE_002872"
     CDS             699..785
                     /locus_tag="ACG9BE_002872"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN3033566.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1087..1311)
                     /locus_tag="ACG9BE_002873"
     CDS             complement(1087..1311)
                     /locus_tag="ACG9BE_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033567.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1677..1961
                     /locus_tag="ACG9BE_002874"
     CDS             1677..1961
                     /locus_tag="ACG9BE_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033568.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1964..2380
                     /locus_tag="ACG9BE_002875"
     CDS             1964..2380
                     /locus_tag="ACG9BE_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033569.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2517..>2674)
                     /locus_tag="ACG9BE_002876"
     CDS             complement(2517..>2674)
                     /locus_tag="ACG9BE_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN3033570.1"
                     /translation="PALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
BASE COUNT          882 a          497 c          430 g          865 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tactcagata aaaagttgtt agtacccttc tgtatccgtt gggagagtaa
      241 ggctcaactg ccccctaaaa agggttctaa aaaatcttta aaaaataaat aaaataaacg
      301 cgcgcgcgcg agcaaaattt cccgccaaaa aatcacccac tttttaaaat aaacaggttc
      361 gcgcatgata ttaaactgcc aatctagctc cctatggtcg ctgtaagaag gagtgatcgc
      421 tacgctctca cattttactt gcagcagtcg cctttaggct ccttcgccaa aaataattca
      481 ttagggactc ctttgtggcg tcctgagagc tcgtctaagc cgttctaaga caagattcga
      541 taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
      601 cccaaggcaa taaaaaactt aggcttattt ttgctttttt attgtataat tgattgtcgc
      661 ctatccgaca gtcttgcgac tggaaggagg tgtgtttcgt gttcactaac tttatttgct
      721 cccttcttgt tgggctttta agcgctttgt tcagttactg gttgaataac cgaaacaaga
      781 agtaaaactt aattaaggcg actcagccca caaaaaaagc cccagagaat ccgacctctc
      841 tggggctttc gtgtgtttcg tgttcactta tttgcaatca aattataaca gaaataaata
      901 tttatttcaa aaatatccta caaaagtcta aaatccgtaa ttatttctta tatgcaacat
      961 ttacaaataa agtcgctctg agagctcgta tttttcgatt taagacgttt tatttagttt
     1021 ttggtgtatt tcattggtcc aagctaaaaa actcttagaa gcgattttag agccatttaa
     1081 acccacttaa ttatattctc catgaaattc gctccatgcg tcaaaagctg cgtctgaaat
     1141 tccgccgtct tcgttcacta gttgcattaa tttttccaac gatcgttttc tttcgggtgt
     1201 catattttta tattccaaca atttttcgtc tgttaaatcg aacttgattg cttgaattgc
     1261 ttgctctaat ttttctaagt tttgaaaata ttcgtaagga aatttttcca ttttttcttt
     1321 ctccagtctc acaatttttc tagaaataaa atcacttctt tgccgtctat actctcaaaa
     1381 aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc atttttaaat ttggttatcg
     1441 attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt gtacccactt
     1501 tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat
     1561 cactttttcg ccatcgtggg tagcttatgt atagcatttt ggcaaaaaac agctatacaa
     1621 atatagtatt tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg
     1681 acattttagt tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag
     1741 aactcacgaa taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg
     1801 aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg
     1861 ataaatttat tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa
     1921 ataatcaaat cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt
     1981 ccaaaaacat gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa
     2041 aaatccagat atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc
     2101 cgataccaac acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat
     2161 ttccatcatg actgaaatca tcgcagaatt tgctgattta tatctaaaag atacaggcgt
     2221 tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg aagctaaact
     2281 aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac
     2341 ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata ggagttattc
     2401 ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag caatcttagt
     2461 cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt ttttatttat
     2521 tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct tgaaaaagta
     2581 aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac tttaagatca
     2641 ttgaagtatt tttttagttc tcgtaatgca ggat
//
LOCUS       DAZAID010000179         2633 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000179 DAZAID010000000
VERSION     DAZAID010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.185"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..626
                     /locus_tag="ACG9BE_002877"
     CDS             120..626
                     /locus_tag="ACG9BE_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN3033571.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="ACG9BE_002878"
     CDS             643..1542
                     /locus_tag="ACG9BE_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFN3033572.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="ACG9BE_002879"
     CDS             1629..2513
                     /locus_tag="ACG9BE_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN3033573.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT          958 a          489 c          515 g          671 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat ttt
//
LOCUS       DAZAID010000180         2500 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000180 DAZAID010000000
VERSION     DAZAID010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.186"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..2350
                     /locus_tag="ACG9BE_002880"
     CDS             197..2350
                     /locus_tag="ACG9BE_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFN3033574.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          859 a          429 c          547 g          665 t
ORIGIN      
        1 taattttcaa aaataagttt attttttaat gaaaaaaata gttggatatg taactttaag
       61 tgattgctgt gaaacaaaaa gttgatttta tcttgaaatt aggtaaagaa tttcttgctc
      121 cgcccatttt ttctttttta ttaagaagtt tttgggtaca atgaagcatg aatgtaaata
      181 ggcggaagga taggaaatga tgaaagattt tatgaaaaaa atattaaata cttcttggtt
      241 agaaaaaata agaaaaggtg cacaagaacc taagaacaaa acgtttcgcc ggtcccatgt
      301 tccttttcgc ttaaattttc tttttttcat tatatttacg ttatttgttg cgttgattgc
      361 tagattgggt tatttgcaga tcgtgaacgg cgaagagatg gaggcgcgag tgaagtctac
      421 ttctacgatt acggtccaag aaagtactcc tcgcggaatg atctacgact ccacaggaaa
      481 agctttagtt acgaataaag cgaatcaggc catcacatat acgagaggaa cacaagttac
      541 ggctaaagat ttgcttgaaa tcgcaacgaa gctgaactcg ctgatcgatg ttccggtaga
      601 tgaaaatctg acagatagag ataaaaaaga tttttggctg gctgatccca aacatttgaa
      661 agaagccaca aaaagacttt cggcaaaaga aaagcaatta gggaacagcg aacaatatag
      721 tgcgatggtc aataaagtca cagctgatga gattaattta aacgaggagc aattaaaaat
      781 tgctaccatc tttaaacgga tgaatagtgc atatgaatta aacactgtct atatcaaaaa
      841 ctctggtgtt actgatcaag agttagctgt tgtggcagaa cgcgcttctg aacttccagg
      901 agtttcgact ggcacagact ggacacgaga atataatgca gcaagttcac tgaaaagtat
      961 tcttggcagt gtgacgactg aaaagcaagg acttccagca gatgaagcag agaaatacct
     1021 agcaaaaggt tattctcgga atgaccgtgt aggacaaagt tatttggaaa aacaatatga
     1081 agatgtcctg caaggaacca aaacccaata tgaagtttcc cttgataatg aaggaaatgt
     1141 ttcgaatcaa aaagaaatct tctctgggga aaaaggttct aatttaatgt tatctatgaa
     1201 tgcagaattc caatcaaaag tagaagaaat tttgaaaagg aattatcaaa cattgatcaa
     1261 caacggcaaa gcacagtatt cgcctggtgc ttatgcagtg gctatgaatc ctcagacagg
     1321 agaagtgcta gcaatgactg gtttcagtca tgaacaaggt tccaaagaaa tcaccgagaa
     1381 tgctttaggc acgattacta gcgcctttga tccagggtct gtagtcaagg ctggtacttt
     1441 gacagcaggc tgggcaagta acgcgatcag cggaaatcaa gttttgattg atgagccgat
     1501 tcgtctgcaa ggtgcttcag aaaaaagctc tgtctttaat cgaagcggac aagttgctct
     1561 tgatgcagtc aaagcatcgg aattgtcttc taatacgtat atgatcaaag tggcgttaaa
     1621 gatgttagga ctagactata cacctggaat gggactacct agtttagatg aagaagctaa
     1681 agcttatcag caattgagag actctttcaa agagtttggt cttggtacaa cgactgggat
     1741 cgatttgccg aatgaatctc ctgggatctc acgttctgtt gattatatga agaagttcaa
     1801 tgcggataat ggcaaagaat ggtatgctcc aggtaacttt accgatttgg catttggaca
     1861 gtttgacaca tatacaccaa tccaattagc tcaatatgct tcaacagtcg ctaatggcgg
     1921 gaaacgagta cagccgcacc tagtcaaagc gatctatggc aatgacgaaa atggaaactt
     1981 gggtgaagtg aagaaagaaa ttggcacaac agtagaaaat acggtcaata tttctgcaga
     2041 aaatatggcg atcctccgtg aaggattcca ccaagttgtc catggtaccg atccttacac
     2101 aacagccaaa ccattagcca gtgcgaaaat ggacctttcc gcaaaaacag ggactgcaga
     2161 aacggttgcg gaaggtcatc cagatattgc gacagtcaat agtaatatcg tggcatacgg
     2221 accaacagac aatccggaaa ttgccatcag tgttgtcttg ccgaacttat tagacgaaca
     2281 agaccacata aacttgacga ttgctaaaga aataatggac gcgtattatg atatgttcat
     2341 ggcaaaataa aaaagaggtc gtgaaaaagc cgcttagttt cgagtatgaa gaaaaatttt
     2401 gtgaaaatag cttctcacat ttttgcttaa atttgacttc ataccgagaa actggctttt
     2461 gaacaccgtt tactcggcaa aaaaggttgt gacaaaaatt
//
LOCUS       DAZAID010000181         2438 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000181 DAZAID010000000
VERSION     DAZAID010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.187"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACG9BE_002881"
     CDS             316..570
                     /locus_tag="ACG9BE_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN3033575.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACG9BE_002882"
     CDS             571..888
                     /locus_tag="ACG9BE_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN3033576.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACG9BE_002883"
     CDS             1447..2382
                     /locus_tag="ACG9BE_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033577.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          884 a          345 c          458 g          751 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagagaaaaa
     2401 tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DAZAID010000182         2390 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000182 DAZAID010000000
VERSION     DAZAID010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.188"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..2059
                     /locus_tag="ACG9BE_002884"
     CDS             197..2059
                     /locus_tag="ACG9BE_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN3033578.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2390
                     /locus_tag="ACG9BE_002885"
     CDS             2059..>2390
                     /locus_tag="ACG9BE_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN3033579.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          860 a          353 c          465 g          712 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZAID010000183         2372 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000183 DAZAID010000000
VERSION     DAZAID010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.189"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            206..1819
                     /locus_tag="ACG9BE_002886"
     CDS             206..1819
                     /locus_tag="ACG9BE_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN3033580.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          747 a          420 c          453 g          752 t
ORIGIN      
        1 tttcttcagc ttttaaaaaa tatacagggt tatccccttc aaagtattat ctatccttaa
       61 aatagaaaaa aatttttagg ttttatccac aaattatttc tctatatttt ctgtctattc
      121 aaaataaaca ataattttcc ggcacaatta tttgctgttt tttaccataa catagtacga
      181 tcaacgtaga aaaggagatg tttttatgaa agttgttgtc attggcggtg tagctggcgg
      241 tccttcattt gccactcgtt tccgtcgatt gaatgaagcg cacgaaatca ttatctatga
      301 acgcggagaa aatatttctt atgcaagttg tgctttgcct tattatttag gaggtgtgat
      361 cactgaccgt gactcgctga tcgaacgtac accagaaata ttgaaaacaa aaaacaacat
      421 cgatgtcttt actaaacacg aagtaacagc aatcgatcct tctactaagc gattaacagt
      481 taaagaccta tccacaaatg aagaaataaa aacagattac gataagttga tcatctcttc
      541 tggtgctaga ccagattatc cggatattcc cggagttttt gaagcagaaa acggctttgt
      601 cctccgtagt gtgacggatg cggatcgaat caaatcgttc cttgaagaaa aaaatccaca
      661 acatgtcgtc attcttggtg caggtgttat gggtctggaa ttagctgaga atttcaagca
      721 tcgcggctta gacgtgactt taatcgatca attgccacaa gtcgctttcc cttatgatcc
      781 agaaattgct aatttagttt atgacaaatt gctgaaagaa ggattagccg ttcatttaga
      841 aacaagagtt actgagatcc gtgataaagg tcgagaaata gtattatcag atggctccgt
      901 cctttctgct gatatgctaa tttttgctgt tggtgtttct ccgaagaatg aagtggtgaa
      961 agcaaccggc atacaattat cagatacagg tcagatcatt gttgatgacc agttacaaac
     1021 caatctgccg gacatctatg cgattggcga tattatcgaa acgactagtg tagtgactgg
     1081 tcagccgatc caaagtatgc tttccagtgc ggccaatcgt caaggacata tgctggcaga
     1141 tattttaaat ggtacgccta tgcgctatcg cggatatatt ggtgcaggtg tcgcaaaaat
     1201 ctttgatcat acagcaagtt atgcgggaat gacagaacat gaattaaaag catcaggcat
     1261 aacaaattat aaaactgttt ttatcactcc ctttgaccat gcctatttct atccagaagc
     1321 tacaagatta aatctaaagc tgatttttga tgcagatagc ggtcgtattt taggtggaca
     1381 agcatttgga gaaaaaggtg tcgataaacg gatgggagaa ctttctgtag caatcaccgg
     1441 aaacttgaca gtctttgatt tgcctgattt ggagttgcct tactctccac cgtattctac
     1501 tacccgtgac ccgttgaata tagctggtta tgtcgcaatc aatcaaatga cgaatatcgt
     1561 agaaacgatc aaagcaagtg atatacccga aaacgatttg aaagaagcgt tctttttaga
     1621 tatacgtgaa cctaataaag caccttctgg cagtatctcg gcaacaaaaa atattccaat
     1681 gaatgagtta cgagatcgaa tcaatgaaat cccaaaagat aaaaaagttt acattacttt
     1741 cagaagagga ttgaatactt atacttctgc ccgaatcttg gcaggtttgg gtatcaaagc
     1801 gattttgatt gaagaataaa aaaacgaccg cggtgggtag ccgcggtcta ggagttattt
     1861 ttacttgagg agtaaaaatg aaaaagtata tagggttgtt ggttggtatg tatatagaat
     1921 agaagataaa tgtgaagaaa ttgtgcatta tttcttcaca tttattaggt tatagtttaa
     1981 gaaagtttaa acattgcgca atttaattat tttagctgtt tcttttcata aaaaaacatg
     2041 ttgtgactct gagacaaatc atagtccata gtgatgtctc tagagtaact acgcctaata
     2101 ttacgaggca tcatattaat ctatcagcac tagtacctaa actagcatcc ctttcgaata
     2161 ttttttctta tataataaag tttgctttca ctcctctaat ctcaccaaat cgactggctt
     2221 gcgttgtgtg atactaggaa ctgattggtt tagaacgact tttctattgt aatcatattt
     2281 ttcgccatcc tcgttgtctt ctaatccttc aatgaatgat ccctaatgtg tgtattaaat
     2341 aatttaattt ttttgttcct atttatttaa ca
//
LOCUS       DAZAID010000184         2364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000184 DAZAID010000000
VERSION     DAZAID010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.190"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..993)
                     /locus_tag="ACG9BE_002887"
     CDS             complement(37..993)
                     /locus_tag="ACG9BE_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033581.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1118..2296)
                     /locus_tag="ACG9BE_002888"
     CDS             complement(1118..2296)
                     /locus_tag="ACG9BE_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFN3033582.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIE
                     SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE
                     "
BASE COUNT          709 a          392 c          403 g          860 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aacttgggag cgtcaataat
     1081 tttgtgtaaa taaattgtcc tcctgcaaaa taattagtta ctcagtaaac attgaaacta
     1141 atgtatcggt tacctgttga aaacctttat ggcttctgtt tagaaatttt tgattgtatg
     1201 tatcaaaaat gctgactaga aagcgttcta gtgattcttc attttgaaac tgctcttttc
     1261 tacggctgta tcttttaatt tgcttattga aagactcgat tagattggtt gagtaaatgg
     1321 ttctacgaat gctaggtgga aaatcataaa aagttaataa gtcttggttt tctatgagtg
     1381 actgcgtcac tttaggatag tttttcttcc atttctcaat catgccggat aagaaggtat
     1441 tcgcttcttc ttttgagtta gcttgataaa cagccttaaa gtcatcacag atttcttttc
     1501 ggtctttgac acgtacttta tgagcgatgt tacgagatac atggatacaa caatgctgat
     1561 attttgcttt aggataaatt tgatggatag tatctttcat gccttttaag ccgtccgtaa
     1621 taaaaagcaa gacttcttga actcctctgg agttaatatc ctgtagcagc tcattccaaa
     1681 cgtatgttga ttcagttgga gcaatcgcat aactcagtac ttctttagtg ccgtcttctc
     1741 gtataccaat ggcaatataa atcgcttctt tggatacggt ttgacgtttt agtggaatgt
     1801 aagtagcgtc cataaaaata gcgacatact tatcatttaa ggctctggat ttaaaggcat
     1861 ttacttcttc agtcagaact ttagtcatgt tggacatggt ttgtggagta tagtgatgac
     1921 cgtacatttt ttcgatcaaa tcagcaattt cagacatcgt aacacctttt tcgaataaat
     1981 ggataatagt ggtttccaat gtatcgtttg ttcttttgta ggctggtaaa gtttgttgtt
     2041 taaactcacc attacgatct ctaggtattt ccaatgttaa ttcaccatat tcggttttga
     2101 ttgatcgaaa gtaagaaccg tttctcgaat tacctgaatt aaaaccagtg cgatcatatt
     2161 tttcgtaatc taaaaaagcc gttaattcag tccgtaggag tgtgtttatc gctttttcta
     2221 agtgcgaacg gaataattca tttaaatcgc ctttagtgac tagagtttgc acaatttctg
     2281 tagtaaaatc attcataggg aagtcctctt ttctgtgaat tggttgtcgt taactttatt
     2341 ctacagaagg gacttccttt tttg
//
LOCUS       DAZAID010000185         2164 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000185 DAZAID010000000
VERSION     DAZAID010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.191"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..540
                     /locus_tag="ACG9BE_002889"
     CDS             91..540
                     /locus_tag="ACG9BE_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033583.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            540..1034
                     /locus_tag="ACG9BE_002890"
     CDS             540..1034
                     /locus_tag="ACG9BE_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033584.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            1048..1479
                     /locus_tag="ACG9BE_002891"
     CDS             1048..1479
                     /locus_tag="ACG9BE_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033585.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            1481..1618
                     /locus_tag="ACG9BE_002892"
     CDS             1481..1618
                     /locus_tag="ACG9BE_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFN3033586.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            1655..1948
                     /locus_tag="ACG9BE_002893"
     CDS             1655..1948
                     /locus_tag="ACG9BE_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033587.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1945..>2164
                     /locus_tag="ACG9BE_002894"
     CDS             1945..>2164
                     /locus_tag="ACG9BE_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFN3033588.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
BASE COUNT          798 a          326 c          465 g          575 t
ORIGIN      
        1 aaattccaag aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt
       61 acttttgaaa aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc
      121 aatgtacagg tttatccaca aactcattgg aatgctatag aaggcaaacc aacaacggtg
      181 aagggagata aaggcgatcc tggccaagca gcaacaatta ccataggaac cgtttctagt
      241 ggttcaaccg cttctgttac gaatgtagga acatcatctg cagctagatt taattttgtt
      301 ttaccaaagg gagataaagg tgatcctgga ataaatgcca caactacagc tgtagctaca
      361 actacagcaa atgggttgat gtcgtccaca gataaaacta agttggatgg aattgcagct
      421 ggagcacaga aaaatccagg taatgctaca actacgacag caggtttaat gtcagcaacc
      481 gataaagtga aacttgatgg attagcgaat attacatttg agaaagtggg gacggtttaa
      541 tggctgatat tgttcaatta aaagaagacg gagttgctaa atacttaaaa acgcatgtag
      601 aagctattga tggtaaagaa gcattggtgc agacagatgg agaccaagcc attgcaggac
      661 ataagaattt ttcaggttct gtaacaatca ataataaacg tgttttgacg actgatgata
      721 acagatatga agtggtaaat ctggttgtca ctaacggtaa tacagggaca gcaaagcttt
      781 atcgtgaagg aaaaacagtt tctatatatt ttgtggcttt aaacggaaaa agtagtggcg
      841 ggaatgattc ggttatttta actgttccag aaggctatcg gacaccaatt agttttgaac
      901 aactggttgg gtcaatagac cgttctactt tgaacagtgc tcaactatct attggcgcag
      961 acggagccat taaatggaga agaaattcaa gttatggatc agcttattca tttgttatca
     1021 cttattcaat ttaagggagg aaatctaatg aaagtagttt acaaatcaat caagccttac
     1081 ggattcgagc aaatcatttt gaacaatcaa gaaaatatcc ctgaaaactg tacagagatt
     1141 aaaccaccag ttcctaactg gagaccaaga tttgattttg ataaaaaaca gtgggttgag
     1201 ttagctactg aagaagagaa aaaagggaat gctgttgata gcgtagatga attggctaat
     1261 attaaggcat tatacgaaac gctgaaggca gaaaatgatg aattgaaaca gctaaattct
     1321 aaagcaatgc taaacaatgt agcaatcaag caagaaaatg tcttattgaa agaaaagtca
     1381 gaaagtttag cgcagttgaa ttcaaaaacg atgcttgctt ctgtacaaaa taccaaggaa
     1441 atcgaagaaa tcaaaaaaca attacaaggt gggaagtaac atgtattcat atgaagatat
     1501 caaactaatg tatgactggg gctgtttcac taacgaacaa gtaatggttt tcgttccatt
     1561 atgcattaca gacgaagaag cagataaaat cattaataaa gataagagcg catcttaatt
     1621 gatgcgtttt tatttaaggt aaaggagttg tcacatgatt aatttagggg aatggggaat
     1681 gatagcagga tcaataaccg ctatcgtttc tttgatttta ttagtaatta gaccgattgc
     1741 tgcatctttc tcgaaaatta ctgagactct ttcaaaagta agccacaatt tagatttgct
     1801 gactaaagat ttagaatcga gcaaatcaga tcgattgatg attcatgaag aactaaagaa
     1861 acacgatgaa agattagata cacatacaga aaaattggtg gaacacacgc aacaaatcaa
     1921 aactttgttt agggagagaa gaaaatgaat aataaaacgt tcgaagtact aaaatggttc
     1981 gcactggtaa ttattcccgc actagctact ttcgtggggt tagttggtaa agcgctcaat
     2041 tggcagtaca cagatatctg tgttgtcatc attactggtt ttggcgcgtt tttagggagt
     2101 gtgttgggtg tatcaaatcg aacctacaaa atgttctcgg ctgaaagcga agaaggagga
     2161 aaca
//
LOCUS       DAZAID010000186         2162 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000186 DAZAID010000000
VERSION     DAZAID010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.192"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..572
                     /gene="ahpC"
                     /locus_tag="ACG9BE_002895"
     CDS             9..572
                     /gene="ahpC"
                     /locus_tag="ACG9BE_002895"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFN3033589.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            582..2111
                     /gene="ahpF"
                     /locus_tag="ACG9BE_002896"
     CDS             582..2111
                     /gene="ahpF"
                     /locus_tag="ACG9BE_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFN3033590.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
BASE COUNT          699 a          371 c          469 g          623 t
ORIGIN      
        1 gttttactat gtcattgatt gggaaaaaaa tcgaagaatt taatgcacat gcttatcatc
       61 aaggagaatt tttagaagtc agtgaaaaag atcttatggg gaactggagt attctttgct
      121 tctatccagc tgattttact tttgtttgtc ctactgaatt agaagactta caagaacaat
      181 acagtacatt gaaatcttta ggtgttgaag tcttttcttg ctctaccgat acccacttta
      241 ctcacaaagc atggcatgat acatcagatg ctatcggtaa aatcgaatac gtgatgatcg
      301 gtgatccgtc tcatcagatt tctcgtattt ttgatgtttt ggatgaagag caaggattag
      361 ctcagcgagg aacatttatt attgatccag acggaatcgt tcaagctatg gaaatcaatg
      421 cagatggaat cggtcgtgac gctagtgcac tgattgataa gattcgcgct gctcaatatg
      481 tacgtaccca cccaggtgaa gtttgtccgg caaaatggaa agaaagtggc gaaacattga
      541 aaccaagttt tgatcttgtt ggaaagattt aggtggtgga tatgttagac aaagaaacta
      601 aacaaaatct cgaacaatat ttagcactga ttgaatctcc tattgttttt tctgttagtt
      661 tggataatag tgaaaatagt caaaaactag ctgaatttac gaaggaaata gcagaaatgt
      721 ctcctaaaat ctcttgggaa aaaagagatt cgatgcgcac acctagtttt tctatcaatc
      781 cagttggcaa agaaagtgga atcgtttttg ctggtatccc cctcggtcat gaattttcct
      841 cttttttact ggctatgtta caaattagtg gacgggcacc gaaaatcagt gaatctttga
      901 gtcttaaaat caaaaaaata acacaaacat tgcattttga cagttatgtt agcttgactt
      961 gtcataattg ccccgacgtt gtacaggcat tgaatatatt agctgtatta aatccaaata
     1021 ttactcatac gatgattgaa ggtgggatgt tccaaaaaga aatcgatgat aaaaaaatca
     1081 tggcagtgcc aactgtgttt ttaaatggag aagaatttag tagcggacgc atgacgatcg
     1141 aacaaattgt tgaaaaaatc actggccctt tgattgaaga agagttgttt gaaaaagaac
     1201 cttatgatgt attagtgatt ggcggtggac cagcagctag tagtgcagct atctatgcag
     1261 cacgtaaagg aatccgtaca ggacttgtcg ctgattcttt cggcggacaa gtcgttgaaa
     1321 cactagggat tgaaaatatg attggtacct cttacacaga aggtccaaaa ttgatgcagc
     1381 aagtagaaag tcatgtgcgt agttacccta tcgacattat gatgaatcaa caggccgttt
     1441 ccttaaataa agagcaacat tttattaata ttggcttagc taacggtata tcacttaaag
     1501 caaaaaccgc catcatcgcg acaggtgcgc actggcgttc tatcaatgta ccaggcgaaa
     1561 aagagtttaa aaataaagga attgcttatt gcccacactg tgacggccct ttatttaaag
     1621 gaaaagagat tgctgttatc ggtggtggta attctggtgt tgaagcagca atcgatttgg
     1681 ctgggttagc aaaacatgta tatgttctag aattcttacc agaattaaaa gcagatcaaa
     1741 tcttacagga taaactagtc tctcttgcga atgtcactgt tatcaaacat gcagcgacga
     1801 aagaaatcag cggtacgaat caggtagaat ctctctccta tatcgatcgc cgtacgatgg
     1861 agatgcatac actagctgtt tcaggagtat tcattctagt cggcttactc cctaatacgg
     1921 attggctaga tggaacgatc gaaatgaatc caagaaatga aatagtgacc gagaaaaatg
     1981 gcgcaacgaa tatccccggc gttttcgcag ctggtgattg tacagatagt ccatacaaac
     2041 aaatcattgt ttcaatgggt tcaggagcta ctgcagcttt aggagcattc gattatctga
     2101 tgcgcacttg agctatgaag attgtaaaaa gtcagagagc ggaaaaaagg atggtgacaa
     2161 ga
//
LOCUS       DAZAID010000187         1998 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000187 DAZAID010000000
VERSION     DAZAID010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.193"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1622
                     /locus_tag="ACG9BE_002897"
     CDS             <1..1622
                     /locus_tag="ACG9BE_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFN3033591.1"
                     /translation="TVDLTDNVTNLPTLPEGTTVTDVTPGGTIDTNTPGNYEGVIEVT
                     YPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPALPEGTTV
                     TDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKV
                     EIGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVK
                     VPVEVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVST
                     AGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIK
                     NKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQV
                     DPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTAT
                     DESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGE
                     SDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1756..>1998
                     /locus_tag="ACG9BE_002898"
     CDS             1756..>1998
                     /locus_tag="ACG9BE_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFN3033592.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVD"
BASE COUNT          789 a          305 c          425 g          479 t
ORIGIN      
        1 gcacagtaga tctaacagat aatgtaacca acctaccaac attaccagaa ggaacaacag
       61 taacagatgt cactcctggt ggtacgattg ataccaacac accaggtaat tacgaagggg
      121 ttatcgaagt aacgtatcca gatggtacga aagatacagt gaaagttcca gtagaagtga
      181 cagacaatcg ttccgatgcc gataaatatg aaccaacagt agaaggcgaa aaagtagaag
      241 ttgggggcac agtagatcta acagataatg ttactaactt gccagcgtta ccagaaggaa
      301 caacagtaac agatgttact cccggcggta caattgatac caacacacca ggtaattacg
      361 aaggtgtcat tgaagtaacg tatccagacg gtacgaaaga tacggtgaaa gttccagtag
      421 aagtgacaga caatcgttct gacgccgata aatatgaacc aacagtagaa ggcgaaaaag
      481 tagaaattgg aggcacagta gatttaacag ataatgttac taacttgcca gcgttaccag
      541 aaggaacaac agtaacagat gttactcccg gcggtacaat tgataccaac acaccaggca
      601 attatgaagg tgtcattgaa gtaacgtatc cagacggtac gaaagatacg gtgaaagttc
      661 cagtagaagt gacagacaat cgttctaatg cagataaata caatcctgaa ggacaaaaag
      721 tgactacaga cttaaataaa gagccagatg catctgaggg aattaagaac aaagaagatt
      781 taccaaaagg aactacttat gcttggaaag agaaagtaga tgttagcaca gctggaaata
      841 aaaaaggtac tattgtagtg acatatccag atggatcatc tgatgaagtt gaagtagacg
      901 ttacagtgac agatactcgt tccgatgcag ataaatataa tcctgaagga caaaaagtga
      961 ctacagactt aaataaagag ccagatgcat ctgagggaat taagaacaaa gaagatctac
     1021 caaaaggaac tacttatact tggaaagaaa aagtagatgt tagtacagct ggaaacaaaa
     1081 aaggtactgt agtagtaaca tatccagatg gatctaaaga agaagtagaa gttactattt
     1141 ctgtagtaga taaaaaagca ccgaataaac ctcaagttga tcctattaca gagggtgacc
     1201 aaattgttac tggtaaaact gaaccaaatg cagaggtgac agttacatta ccagacggaa
     1261 gtcaacacca tgggacagct gataaaaatg gtaattttac agttaaagtt cctaaattag
     1321 aggcagggac aaaagtgata gtaactgcaa ctgatgaatc tggtaatact agtgaaccta
     1381 ctaatgtagt tgtttcttcc aacaaaaaag acagtggaaa aaacggtagt aaaggtagta
     1441 agactgacaa tcaaggtagc aacagcaatc aagacaaaaa tagaggcaaa agccagtcct
     1501 caaaagtttt tcctaagacg ggagagagtg acagtaatat ctttactatt agcggtggtt
     1561 taatactgtt aggaacgtta ggattattag ggtataaaaa tcgaaaaaaa gagaatgaat
     1621 agaaaatcaa tttcttaagt cttctctgag aagaggaaga atctttttct tcctcttttt
     1681 gcttttttat gatgggaaat aagtgttagt taaagatgaa tatacttttt atagaaagga
     1741 aaagaatgta attaagtgga aaaaatagta attattggtt caaaccattc agggctagcc
     1801 gcagctcgat ttctaaactt aagcgattca aattatgaag ttattctaat cgataaaaaa
     1861 tcagaaatta gttacttgga atgtgggaca tcgttattta taaaaggaga agtaagtaat
     1921 ttagaagatc ttttttatca aaatagaatg acactaaatt ctgagataaa aaagttattt
     1981 ctagagacag aagttgac
//
LOCUS       DAZAID010000188         1983 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000188 DAZAID010000000
VERSION     DAZAID010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.194"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACG9BE_002899"
     CDS             complement(<1..426)
                     /locus_tag="ACG9BE_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFN3033593.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ACG9BE_002900"
     CDS             complement(535..1566)
                     /locus_tag="ACG9BE_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN3033594.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          612 a          327 c          341 g          703 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg aagatttatt
     1981 cta
//
LOCUS       DAZAID010000189         1974 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000189 DAZAID010000000
VERSION     DAZAID010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.195"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..424)
                     /locus_tag="ACG9BE_002901"
     CDS             complement(<1..424)
                     /locus_tag="ACG9BE_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3033595.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSL"
     gene            complement(421..1074)
                     /locus_tag="ACG9BE_002902"
     CDS             complement(421..1074)
                     /locus_tag="ACG9BE_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN3033596.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1362..>1974
                     /locus_tag="ACG9BE_002903"
     CDS             1362..>1974
                     /locus_tag="ACG9BE_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFN3033597.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          621 a          351 c          253 g          749 t
ORIGIN      
        1 taaggcttaa atctttcgca tctttttgca tatcagctgt agaaacaaat tgtagatttg
       61 tttttttacc atcattaagc tttatttctc tttcttcaat tatcaaagaa ttactatcac
      121 tttctaaatt aacatttata ttatctttga tttgtatttc attcttatta tttttttctt
      181 ttatgaatac ttttatttca ctactcttgg aataaaactc caaagtttgt tctatatatt
      241 ttatttcttt tccattcata ttactagaaa tttcatttgc catattatga atcttttctt
      301 ttctcgtctc caaatatgtt ttaggaaaga taaaaaaaat taatgaatga actaatataa
      361 ttattattcc aagaacagaa aatatttgta tgaacatttt tggaaatatc tttaattttt
      421 tcattttctc tccaatttat atcctacatt tcttatagta gttatacagt ctaattgtaa
      481 tttttttcta agttctttta tatatacatc tattactcga tcataaggag tttcttcgct
      541 atctttccat acataatcaa taatttgcat tcttgttaac acttgtccat tattatccaa
      601 taaacatttt aatacttcta gttcttttgc atttacatct atttcttcac catttatttt
      661 agctgtatag ctattaaaat taaccgatag atccttatat tcaaacttct ctaaatgtcc
      721 ataattcttc ttaattaaag aatctattct agcttttaag accggcaatg aaaatggctt
      781 ttctacatat ccgtctacta aattagtaaa tgcatcaatt ttatattctt catcactaaa
      841 tgcagtcaag attagaattg gtaaattgct tttctttcta atttctttca acacttctaa
      901 accatttata aaaggtatct gaatgtctaa gataaccaaa tttatatcgc tattaaattt
      961 tgacaaggct tctcttccat ctttagcttg aataacagta tatccaaatt ctgaaagata
     1021 ttcacttatt ccctctctta tcatattatc atcttcaaca gttaatattt tcatatatac
     1081 ctccacttaa gcctatacac tagatattat acagcatatt atggctctat aatatctagt
     1141 gttggtgaca ccttctgttg ccaatacttt ttcttttttc taataaatgc aaacagacac
     1201 cttgaaatcc tttagaataa agggttctat aatattttgt gttgttggaa aatcgaaggc
     1261 gattttctac taacactttt tcttcgtttc aactcaaaaa gagactcact tttggtaaaa
     1321 tttagtcgac caaaacatca ccctaccaaa ggagtcgctc tatggataat catactagaa
     1381 aattactcgt attaacagat aaacatttat tttttgatga agaatggctg attgaaaaag
     1441 aatataaagg cgttcaagcg cagtttatta aagggagatt ggatgactca ccttcccatt
     1501 gtgaacactg tggatcgatt cgtattatcc gcaacggttc ctatacgaca cgtactcaga
     1561 tattgaaagt caaagaaaag ttgactattt tagaattaaa acgtacccgt tttctatgct
     1621 atgattgtgg gcagaccttt tctgcgaaaa cggacttagt tgatgaacat catcaactaa
     1681 caaaggaact gaagcaggcg attctcatag aactgtacga aaatcaatca cgtaaactaa
     1741 tcgctaagaa gtacttcgtc tcggatggga cggtgacacg gatcttgcgt gaagcaacga
     1801 agcattacca gccacgtatg aacttccttc cgacggtgtt gtgtatggat gagttcaaat
     1861 caatgaagtc tgtttctggt tccatgagtt ttatttgtgt tgatgggaca acccatcagc
     1921 tatttacgat ccttgaagat cgcagacttt acaaactaac tcaatacttc ctgc
//
LOCUS       DAZAID010000190         1952 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000190 DAZAID010000000
VERSION     DAZAID010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.196"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..573
                     /gene="lepB"
                     /locus_tag="ACG9BE_002904"
     CDS             22..573
                     /gene="lepB"
                     /locus_tag="ACG9BE_002904"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN3033598.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(656..1198)
                     /locus_tag="ACG9BE_002905"
     CDS             complement(656..1198)
                     /locus_tag="ACG9BE_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFN3033599.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1435..1833)
                     /locus_tag="ACG9BE_002906"
     CDS             complement(1435..1833)
                     /locus_tag="ACG9BE_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN3033600.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT          692 a          342 c          324 g          594 t
ORIGIN      
        1 agagggatgg attgatagag attgacaaaa aaacaacgct atattaatcg cttctggctt
       61 gtattcaaat acttacttgt ttctgtattc cttgcgttca tgttgcgggg atttttattc
      121 atcccggtac cagtggaagg caattcaatg gaaaacgtgt tgaagcaagg tgatatggtt
      181 gtgatggaaa aattttcgga gatcagacga tttgatattg ttgtttttca attagcagat
      241 ggcacgattt atatcaaacg agtaatcggt cttccaggag aaaatgtcag ctaccaaaat
      301 gatcaattga agatcaatgg gaaagtagtt aaggaacctt atctaactaa aaacctgaaa
      361 tcagatcatg caaatgcatc ctatacgacg gactttacac tacaagagct aacgggacaa
      421 tccaagcttc cggaagataa ttattttgta cttggagata atcgaagagt ttcaaaagat
      481 agtcgttcgt ttggcacgat caataaaact gatattctag gtaaagcaag atttgtctat
      541 tatccacttg atgaaataaa atggatccag tgagaaacaa taaagcgtgc agtctgttga
      601 gactgcacgc tttattgttt aatccaaaat cagtttacta ccttagtacg taaaatcaaa
      661 cagctcgttt aaacactttt ctagcctcta aagttttgat aaatgaataa acaaccatta
      721 tttgaatagg gatttggatc atattcctga tgaatctacc tagaaaaatt actttgaaat
      781 ctgcaccaaa caaaacagac atcagccaag aattgataaa aaggctgact gggatcataa
      841 tcaataattg ggcagcgatg acggaaggca ggtttatctt tttatgatat aaaaataagc
      901 cattaataaa tccaccaagg aaagccgtga taagaaaagc agcttgaaac gttcctgaac
      961 caaatatcgt gaaatttaaa gtgttcgaca acatttcaag caaaccagcc cagatcggtc
     1021 catatagata acccataagc gccatactta aaaatccgaa tgtgatttga aaataagcag
     1081 gaccaatcgc tatcttgtat aagatgacat caagggctaa taaaagagca gaggtcgaca
     1141 acaatcttgc attcatttcg ggcaatctaa aaacgtcttt tgcaatcaaa gtgttcaaaa
     1201 tattctctcc tttgtcagtt aataataaaa ggataccata aaatcaccaa tgtgtcatga
     1261 cacattggtg atttacatac actggcatct aacaattaaa aaaagggtga gaagactgct
     1321 tttataaact gtaaaaaaga gattgtacga ataaaaaaaa tagaggcgga gaatacttat
     1381 cgtatcatcc gcctttgttt ttttgttata aaaaacgtca gattagttat ctgcttacat
     1441 cttcatccat acgggttcta atgtatttcc gtctagatct tggacttcta atgaaaacat
     1501 ttcttcttca ggcattccca tatctacttc gtggaaagta ccaccatttt cttttgcaat
     1561 ttgagcaaat ttttttactt catccgcatt ttcaacacta aacgaaatca aagcaccact
     1621 tgttttttta ttatcagcta tttctttatc ttttataaat tttttataaa aatcatgaga
     1681 gagaagcatt atccaaaaag tgtcatccca taccattgaa ccagcttctt cagtagaaaa
     1741 ttcttcattt tttttaaaac ccagcttttc ataaaaagca acggaacgat tgacatcagc
     1801 aacaggaaaa ttaacaaaaa ccatactatt catttgtttc gtctcctttc tgttttgttc
     1861 gtctatcatc tcttatttag gaaacgctgt caaaaattta gattcatata tagaaaccaa
     1921 aataaaaatt tgcatcatca aaaaagtgaa ga
//
LOCUS       DAZAID010000191         1939 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000191 DAZAID010000000
VERSION     DAZAID010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.197"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="ACG9BE_002907"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="ACG9BE_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN3033601.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="ACG9BE_002908"
     CDS             853..1041
                     /locus_tag="ACG9BE_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN3033602.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="ACG9BE_002909"
     CDS             1054..1611
                     /locus_tag="ACG9BE_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN3033603.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="ACG9BE_002910"
     CDS             1643..1885
                     /locus_tag="ACG9BE_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN3033604.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          714 a          299 c          399 g          527 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagc
//
LOCUS       DAZAID010000192         1878 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000192 DAZAID010000000
VERSION     DAZAID010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.198"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..1867)
                     /gene="uidA"
                     /locus_tag="ACG9BE_002911"
     CDS             complement(59..1867)
                     /gene="uidA"
                     /locus_tag="ACG9BE_002911"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFN3033605.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT          579 a          388 c          233 g          678 t
ORIGIN      
        1 caaaaaaaaa cgattaggct aggctaatcg ttttttgaac attcttaaaa aatccaaatt
       61 attttttata actgaaattg ggtattgatg accatcgttt ctgtagatag taagcaatca
      121 ttttaggttt tctatctcta gtaaagattc ctttcttatt tccttgcact cttaaaatcc
      181 cttgacttgt tgcaaaatct gcaaaattcc acacttgttc accaacaaaa ttttcacatg
      241 tatctattac ttcgtgattt gcttcgtaat aagccacttg atactcttct gtaaacattg
      301 ttggtactgt atcatgaaat ccagctactg tatctgctcc atattctgta aacattattg
      361 gcgtatcagg acatctttcc atccatccat ctagctcttt tttcaacaga tattttgcat
      421 gtgccagatc ccctcccgaa acataccaac cataataacg atttaaacat aaaacatcga
      481 caatatctcc taccttacaa acatctggcg ttgattttag ataagtaacg attgttacag
      541 gtcttttctg cggatcaagt tctttagtta attcaactaa aggttcaaaa tattctttag
      601 caccttcgga atccgaatct ggttcatttg caatagacca cattacaaca cttgggtggt
      661 tcttgtcgcg ttctatcaat tccttgatca ccgttttatg agaattttct gttcgtatct
      721 ctttccacgt attatgtttt tccacattat catctaataa agtggccatg aaatttaaat
      781 gtaaccctac tgcaggtact tcatcaataa ctacaatacc ttctcgatct gctaatcgca
      841 taatttcttc tgagtaagga taatgtgatg ttctaaaaga attagcacct atccatttca
      901 ttaaattaaa atctttaata tttacagcct cattcaaacc acgtccatta acatacgaat
      961 cctcatgctt accaaatcct ttaaaataaa aaggtttatt attaatcaaa aatttgccat
     1021 tggtaacctg aacactgcga ataccaaatt cttctgtata ggtatcgatc actttattat
     1081 catcaattaa actaattttc atactatata aataagcgtc tagaggttcc caaaaaattg
     1141 gattcttaag ctgaatcatt cctttctttc cttcagcttc agctacaagt tgagcctctt
     1201 cgtcatatat cgctattaag atttttccgg tccattctgt atcgatattg aattctacac
     1261 ttgcatccaa atcagttatt cttgtattaa tagtaatatc acttatatag tctttcggag
     1321 ttgtatataa cttaacaggt ctatgaatac ctgcataatt gaagaaatca aaattaacta
     1381 agttttcctt ttttatatcc ccattttcct gtactgtttc cttcataatc cccacaggta
     1441 aagtactatc gtcaataata ttgtttacag caactgtaat tcgattattc ccgtctttta
     1501 caaaccgatt aatttcagac tcaaatggtg taaacccacc tttgtgttga gtaataaact
     1561 ttccattcaa aaaaactttt gcttcatgtg ttgcagatcc aaaacgaaga actaatcttt
     1621 cttctaataa tcttttagga agagtcactt ctttttcata ccacacccat ccaacatgat
     1681 ttctaatgtt ctctgtttca aataaatcat tataagaaga gggaactgaa atcggataac
     1741 tctctttttc atttattggt tctaaagcaa gttcttcctg taagtcatca tttgtatcta
     1801 atttaaatct ccagagaccg cttaagtcaa ttacttgcct cgtctctgtc ataatgggat
     1861 acaacatata aaccactc
//
LOCUS       DAZAID010000193         1743 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000193 DAZAID010000000
VERSION     DAZAID010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.199"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..>1743
                     /locus_tag="ACG9BE_002912"
     CDS             4..>1743
                     /locus_tag="ACG9BE_002912"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HFN3033606.1"
                     /translation="MKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGSSR
                     DKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGACTD
                     GTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRS
                     FGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWDNS
                     FGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGKLG
                     FNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKGII
                     TDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQAIT
                     LVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFEVE
                     KYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIVWP
                     ASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLMGL
                     SEFKGVSPIDAYCG"
BASE COUNT          624 a          225 c          351 g          543 t
ORIGIN      
        1 actataaaag tagatttacg aaaaaaacca tataatttag atgaagatgc tatttcttgg
       61 attgaagaga cgattgattc tatgactttg gaagaaaaaa taggtcaatt gtttgttaat
      121 atgggatcta gtcgtgataa aaattacttg agtaatatgg tcaatcagta ccatattgga
      181 gctgtacgat ataatccagg aactgctgat gaggtatatg atcaaaacta tatcttacaa
      241 acagaaagta aaattcctct attaattgct gctaatacgg aagcaggtgg taacggagct
      301 tgtacagatg gtactgaagt tggtgtagaa gtaaaaattg gtgcaacaaa tgatgcgaaa
      361 tatgcttatg aattagggag agtttctggg attgaagcat cggctattgg atgtaactgg
      421 agttttgctc ctattgtaga tattaataga aattggagaa atcctatcat ttctagtcga
      481 tcttttggac aaaaccctga taaagtttta gaacttagtt tagcttatat gaaaggtatt
      541 caggagagtg gaattttgcc tgcagctaaa catttccctg gtgacggtat tgacgaacga
      601 gatcagcatc tatcttttag tattaatagt ttaggtacgg aagaatggga taattctttt
      661 ggaaaagtat actcaggctt aatagaagct ggattacctt caattatggc aggtcatatc
      721 catatgccag cttattcaaa acattttaat cctgatctga aagatgaaga tttacttcct
      781 gcaactttat caaaagaatt aatcactgac ttacttagag gaaaactcgg atttaatgga
      841 ttagtagtta ctgatgctag tcatatgtta ggaatgactg gggcaatgaa acgatcagaa
      901 ttattgccca cttctattgc agctggatgt gatctatttc tattttttaa tgatccagat
      961 gaagacttta attatatgat ggaaggctat cgtaaaggaa ttatcactga tgatcggtta
     1021 ttagatgcct taaaaagaat tttagggtta aaagctaaat tgggattaca caaaaaagca
     1081 aaagaacaaa ttttattacc taagcaagaa gctctggaaa aaattggttt aattgaaaat
     1141 aaatcaattt ttaaagaggt ggctgaccaa gcaattacat tagttaaaga taaacaagat
     1201 atttggccta ttgtaccaga aaaatataaa aggattttac tagtagatgt acatggggta
     1261 aaaggtgggt ttggtgaagt agttgctgga aatagacctc aagccattga tttattgaaa
     1321 cagattttgt cagcaaaagg atttgaagtt gaaaaataca tttcacctga agaagaattg
     1381 aaaggattat cgcaagaaga aaaaattaaa aatatagctg gaatatatgc tgctaagaga
     1441 ccaattaaaa atttaactaa aaaatatgac ttgatcatta atatagctaa tgttaatcca
     1501 ggcacagttc aacgaattgt ctggccagca agtaaaggaa ctccggatat accgttttac
     1561 attcatgaaa ttccaacaat ttttgtatct gtacagtgtc cgtttcattt ggctgatgta
     1621 cctcaagtaa agacttatat taatacttat gatggaaaac aagaaacttt agaaatattg
     1681 gtggacaaac taatgggtct ttctgaattt aagggagtaa gtccaataga tgcttattgt
     1741 ggt
//
LOCUS       DAZAID010000194         1688 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000194 DAZAID010000000
VERSION     DAZAID010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.200"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..322
                     /locus_tag="ACG9BE_002913"
     CDS             23..322
                     /locus_tag="ACG9BE_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN3033607.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(358..729)
                     /locus_tag="ACG9BE_002914"
     CDS             complement(358..729)
                     /locus_tag="ACG9BE_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033608.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(731..1132)
                     /locus_tag="ACG9BE_002915"
     CDS             complement(731..1132)
                     /locus_tag="ACG9BE_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033609.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(1146..1553)
                     /locus_tag="ACG9BE_002916"
     CDS             complement(1146..1553)
                     /locus_tag="ACG9BE_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033610.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          546 a          282 c          228 g          632 t
ORIGIN      
        1 aaaacagatc ggagtaatgg ttatgctttt taatgagaca caattatggt ttaaatttga
       61 cccttcgaat agatttatca aagattttta taaagtatgg aattcagaag ttttcttttt
      121 agcaatcgaa gatagcttat taatcaatct ctactattct aataagaact atttcaaaat
      181 tcctgctgcg aaaactagaa tgaagaaggg tgtatacttt ttgtttgata tcgtgactga
      241 cgtgccggac gcacgtagcg atcatcggcg ttatgactat ataaagtata ctttcgttga
      301 tccagaaaga tacaaagatt aaataaaaaa ataacaaaca aactcgcttg aaattcatta
      361 ggtgagtttg tttattattt tttctgcttc tcgattcatc agcgccatat ctgttttcgt
      421 aatggtttca ttaggaatat agtcagcaca ttttcttttc tctaacattc tatacccact
      481 attatgtatt cctaaggtat tcaattcctt aactgttgaa aacaaattcc aaacggtttg
      541 gtggcttcct tttccgctac ttccattata aataatatca taattttctt tcaaatactc
      601 atcagatttt ttaaaagcac tgtagtatgc tcttgaaaca gcagttcttc tataagattc
      661 tttagaatta tatttatcat ctgattcaat ttgtttggaa atagataaat attcattaaa
      721 gtcaaacatt ttattccccc aaagttgcta ctacagattt ccccatatat ttatagtcag
      781 ataatacatt atcgagagta tccaaaacat cgtaatctga ggaaaacaat tcagatgttg
      841 ttttgataac aaagaaatac aagtttaaat cttcatcata ttttatattg atttcagctt
      901 ttctttttaa aaccgtattt atcacatcaa aaattttgcc atcattactt gaaattgtgt
      961 taatctcatt ttttgtagta attacaagtt gattatcatt tttagttttc ggagtagaac
     1021 ttgcatccac aacaactgga gatgctagaa ttaccgatgc gcaagcaaaa atcgcagcaa
     1081 aatttattaa gctaccactc cgatgtgtat ttgatggtac tatatcaagc atccgtatcg
     1141 tctccttaat ttttttccac tggtttgttg accttgatat caaagatttg ccttccaatc
     1201 tcagttccat taaatttaac aacagcataa tgctctcctg cttcttcaac caagacattt
     1261 ctggcgttta tattaaagtt tatatcgtta acttgaggag gcaagcttac cgatacttca
     1321 cccgtagaaa aaatttgttt ttcattcttg tttaaaactt caatttctac tatttttatc
     1381 ttttctgtat cctctatctc agacaccaca gctgttacag aaaatgataa agcagtagga
     1441 attaaaggcg cattaataga aagagctgga ttaattactt tattaataac attagtgcca
     1501 aattgctgac tttctacatc ttctgaaatt attaattgta ctctataaga cattgatttc
     1561 tcctaatttt tacatttatt taataaattt atgtttcact tttgctagtc aatttagttt
     1621 aacaatagta acaaagcttt gtcaacggaa atattagcta attttgtaag catatttata
     1681 agtatatt
//
LOCUS       DAZAID010000195         1655 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000195 DAZAID010000000
VERSION     DAZAID010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.201"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACG9BE_002917"
     CDS             <1..1604
                     /locus_tag="ACG9BE_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFN3033611.1"
                     /translation="ETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHK
                     MFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPI
                     REGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYN
                     GDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFS
                     RTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQA
                     YGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQ
                     IQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTY
                     AANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQAS
                     DNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFK
                     YPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          608 a          269 c          339 g          439 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagtaaag tggaa
//
LOCUS       DAZAID010000196         1647 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000196 DAZAID010000000
VERSION     DAZAID010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.202"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG9BE_002918"
     tRNA            complement(20..91)
                     /locus_tag="ACG9BE_002918"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG9BE_002919"
     tRNA            complement(113..200)
                     /locus_tag="ACG9BE_002919"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG9BE_002920"
     tRNA            complement(225..298)
                     /locus_tag="ACG9BE_002920"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACG9BE_002921"
     tRNA            complement(312..382)
                     /locus_tag="ACG9BE_002921"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACG9BE_002922"
     tRNA            complement(384..459)
                     /locus_tag="ACG9BE_002922"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ACG9BE_002923"
     tRNA            complement(478..551)
                     /locus_tag="ACG9BE_002923"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="ACG9BE_002924"
     tRNA            complement(564..653)
                     /locus_tag="ACG9BE_002924"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="ACG9BE_002925"
     tRNA            complement(677..750)
                     /locus_tag="ACG9BE_002925"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="ACG9BE_002926"
     tRNA            complement(764..837)
                     /locus_tag="ACG9BE_002926"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="ACG9BE_002927"
     tRNA            complement(865..937)
                     /locus_tag="ACG9BE_002927"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="ACG9BE_002928"
     tRNA            complement(957..1030)
                     /locus_tag="ACG9BE_002928"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
     gene            complement(1040..1113)
                     /locus_tag="ACG9BE_002929"
     tRNA            complement(1040..1113)
                     /locus_tag="ACG9BE_002929"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Arg,seq:acg)
     gene            complement(1124..1209)
                     /locus_tag="ACG9BE_002930"
     tRNA            complement(1124..1209)
                     /locus_tag="ACG9BE_002930"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Leu,seq:taa)
     gene            complement(1226..1297)
                     /locus_tag="ACG9BE_002931"
     tRNA            complement(1226..1297)
                     /locus_tag="ACG9BE_002931"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1263..1265),aa:Gly,seq:gcc)
     gene            complement(1309..1381)
                     /locus_tag="ACG9BE_002932"
     tRNA            complement(1309..1381)
                     /locus_tag="ACG9BE_002932"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Thr,seq:tgt)
     gene            complement(1399..1480)
                     /locus_tag="ACG9BE_002933"
     tRNA            complement(1399..1480)
                     /locus_tag="ACG9BE_002933"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1444..1446),aa:Leu,seq:tag)
     gene            complement(1488..1560)
                     /locus_tag="ACG9BE_002934"
     tRNA            complement(1488..1560)
                     /locus_tag="ACG9BE_002934"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1525..1527),aa:Lys,seq:ttt)
     gene            complement(1564..1636)
                     /locus_tag="ACG9BE_002935"
     tRNA            complement(1564..1636)
                     /locus_tag="ACG9BE_002935"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Val,seq:tac)
BASE COUNT          438 a          460 c          391 g          358 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaattaac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
     1081 gtcaaacact ctatccagtt gagctatggg cgcatatttg aaatgccgag gaccggaatc
     1141 gaaccggtac ggtgatcact caccgcagga ttttaagtcc tgtgcgtctg ccagttccgc
     1201 caccccggcg cgattgcttt ggcaaagcgg aaaacggggt tcgaacccgc gacccccacc
     1261 ttggcaaggt ggtgctctac cactgagcta tttccgcgtg tttaaagatg ccggctaaag
     1321 gacttgaacc ctcgaccctc tgattacaaa tcagatgctc taccaactga gctaagccgg
     1381 catgtaaaac ttaaattatg cgggtgaagg gacttgaacc cccacgccgt aaggcgctag
     1441 atcctaaatc tagtgcgtct gccaattccg ccacacccgc aaaaatatga gccgtacagg
     1501 gctcgaacct gtgaccctct gattaaaagt cagatgctct accaactgag ctaacggctc
     1561 atatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
     1621 gctgagctaa acctccatgg ataactt
//
LOCUS       DAZAID010000197         1619 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000197 DAZAID010000000
VERSION     DAZAID010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.203"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..>1619
                     /locus_tag="ACG9BE_002936"
     CDS             157..>1619
                     /locus_tag="ACG9BE_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3033612.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVSICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLT"
BASE COUNT          596 a          224 c          289 g          510 t
ORIGIN      
        1 ttccattttt tgcaatattc tcaaaaaaaa gataaaaaat gagaataact gatgaaaaaa
       61 caagcataat ccatcataaa atcagtatag gtgaaaagat gaactttcat ctgcatatca
      121 agtaattggt atgaggcatt ttttgaggtg taggatatga agttggatta ccttatagaa
      181 gaggaagata gagaaaaact gctgctagct agatatttag aatttgagaa aaagaattat
      241 gtagtgctgc ctaagttata tgaaacatta ggtttgtcta aatttaaaac aagaaattat
      301 atcatagaat taaataaaga atttactgaa ttcaaaacaa agccagagat ccaaatttca
      361 gaagaagcag agatccatgt cgaacgtctg aatttgacgg atatcaaaaa tttaagaaga
      421 atctactttc aacgatcaaa aattgctcga ctgttaacgg agatcgtgga ttcaaaagta
      481 acgatcgaaa aatttgcaga gaatcatttt ttaagcatat caagagccta tgtaaaaaga
      541 agagaattag tctcttttct tgaacagcat caaataaagt taaagaaaaa catattagtt
      601 ggggaagaaa taaacatacg gaacgtatta tttggggtat atttcggggt ctttaatggt
      661 ttttctatgc catttagaga aagcgtcatg aatgaagcaa agagtatcgt gcaatatttt
      721 gaatatctgt tcaatttaaa actgtctgca acaaaacgaa tcaaattcaa tttgctaacc
      781 actattagtt tgttgagggc ctatgggaat caaccaatcg gaaaacgttt cttttcagat
      841 gaatacttta agagctcaga aggaatgaag atcatcaagg atttatcaca tatttctttt
      901 cttcatatcc ctaaagagca gatcattgat gagatttctt atatcatgtt gtttttgatc
      961 acggaagaag aatttgaatt agatggcgca tttggacttg cggaagctca ttttcatgat
     1021 atccacatga ttagtcaaaa aatgtcagtg gaattgattg atcaattacc attttcgatt
     1081 gaaatctctg aagaagaaag actatatata cgaaaagaac ttcgtatggg tatgatgcat
     1141 gtcaatcgaa aaaattggat attcgacttt gagatgacta gctttacgtc taaaaagcag
     1201 ctacagtttt tcttcgagac gtatccaata ttcagtacag caatccaaaa ggtcatacaa
     1261 gagaataaac gctatttctt gggaaaagat gaacgtttca tgattcgctt attttatgat
     1321 tatctctttt tattggtcag tatttgtccg gtatcatatt ttgaagcacc gattcatgtc
     1381 tgtattgatt tttcgcaagg atctgcttat accgattaca ttatttctca agtgaaagga
     1441 tttaaaaatt acaatattgt gattgagagt cgaatcactt ctcgtacgaa tatttattta
     1501 tctgattgtt tacttgaaaa atttaataaa gaacagataa tttggaaaaa tccaccgact
     1561 ccagatgact gggaatattt tggaaatacc atcatcagag aaaaaaatag acatttaac
//
LOCUS       DAZAID010000198         1580 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000198 DAZAID010000000
VERSION     DAZAID010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.204"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..235
                     /locus_tag="ACG9BE_002937"
     CDS             <1..235
                     /locus_tag="ACG9BE_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033613.1"
                     /translation="KKYICQPIGLKQKVIGEVISKLENCVVICVDEYHDVDRNEILEK
                     CGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(597..836)
                     /locus_tag="ACG9BE_002938"
     CDS             complement(597..836)
                     /locus_tag="ACG9BE_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033614.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1122..1436
                     /locus_tag="ACG9BE_002939"
     CDS             1122..1436
                     /locus_tag="ACG9BE_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033615.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          540 a          216 c          277 g          547 t
ORIGIN      
        1 aaaaaaatat atttgccaac ctattggctt gaaacaaaaa gtgattggag aagtaatcag
       61 taaattagaa aattgtgttg tgatctgcgt agacgaatat catgacgtcg atcgcaatga
      121 aatattagaa aaatgcggaa aagtagtggc aaaatatact cacgtttatg agttggaaac
      181 aacagaatat ctatataact atagcaacaa atatgaacat actttagttt cataaagatt
      241 taaataaagt gccttcaaaa gcgagaagac caagcttttg aaggcacttt atttagttgg
      301 cagtgattgt tcatgatttt tgatttattc aaaaggttcg cctagagaat ctgtctctgt
      361 aacgaggcct ggtggagaaa ctcgataaaa acctatactg ccagaactgt cctattttcg
      421 gatagacaaa ctagtagttt taaaaagatc atccgatcca atcatttcaa aaaatgcaaa
      481 actgatatca ggatcattta gctcaggctt gttatcgtaa agcatttgta aaggttgcat
      541 ttagttagga gtaaacgcga aactatcatc attatttgct gaagaagatg aagaagctaa
      601 ttcagagttt gtttgaaata aaggagacgt gctgacagtt tctttaccct gttttttgca
      661 gaggtagaaa cttttatatc ggaagtttct accggagaat ttttctcatt tgtagaaatt
      721 gttgaatcga tactattcga aatttgcgta tctgaaggta tgttctttga tgcagaagtc
      781 ccacaagcag taagtaacaa taaatttgat aaactgataa acagaattct tttcattatg
      841 tacacactcc ttatagtatc gtttagcata cacctatgct cagtattagg caaaatcaat
      901 gcaaaactat caagtctttt atagtatcta ttagtcttag taatacatat atggagaggg
      961 tgatatccca gttcaaacca gatacattgg ttaccatctg tattaacgat tatgcctcta
     1021 aacgcttttt ttatgaaaaa atttataagt gaaaacgtag gtatgatatt atctagaggt
     1081 aatttttttg ggtatttaaa tagttgctag gaggatagct tttggatttt tacagtaatt
     1141 ttatcttaat aatagcaata ttattacttc taaatatttg gttttttgat aagagtagaa
     1201 atgcagggat aggttttaga accaaacgaa gtacgtcttc tgaaaaaaag tgggtatatt
     1261 cacagactat tttttacgga ggggttattt caatcagttt actttcttca acactttatt
     1321 cttttaatgt tatagacgtt tccatgtcaa attttatatc tattattgga atccttattt
     1381 ctgcaattat tacacaacta cttcttgtgt ttgaagagaa gtcaaaaaat aattaaagca
     1441 attatgcctt ttagtacagg aattattact tcttacaaat gtatagcgaa tgtgaagcct
     1501 gctgttttaa ttgttctaga aaaacttttg agtgaacaat tattttatgt taggatgaat
     1561 taatttctct agagtttata
//
LOCUS       DAZAID010000199         1533 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000199 DAZAID010000000
VERSION     DAZAID010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.205"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1488
                     /locus_tag="ACG9BE_002940"
     CDS             22..1488
                     /locus_tag="ACG9BE_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFN3033616.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          422 a          289 c          326 g          496 t
ORIGIN      
        1 taaagaattg gggtatagat tatggaaaaa cagcaagaca agggcttctt cgggcagccg
       61 aagggattgt caactttgtt cttcactgag atgtgggaac gatttagtta ttacggtatg
      121 cgtgcattac ttatctatta tatgtacgat acagttgcca acgggggact tggattacca
      181 cgagcaacag cattagctat catgtcgatc tacggatctt tgatctacat gtctagtatc
      241 gtcggcggat gggtatctga ccgagtgtta ggagcaaaac gcactgtatt cttcggtgga
      301 gttttcatta tgcttggcca catcgtcttg gctttaccat ttggtgtaag tacattgttt
      361 gtttcgatgg cattgattat tatcggaacc gcctttttga aaccaaatgt ttctggcatg
      421 gtcggacatc tttattcaaa gactgacttg cgaagagatg caggattttc gattttttac
      481 atgggtgtca acttaggggc attattagca ccactaatcg ttggtacaat cggtcaagaa
      541 tataactatc atttagggtt ctctttagct gctattggga tgttctttgg attattacaa
      601 tacatcatcc aaggacgcaa gacattagac ggagtgggta caacaccgcc gaatcctttg
      661 acaactgaag aacgtaaagg attcatccgt aacttgatca tcgcacttgt tgttatcgct
      721 gcagtattcg gcggggcaca actaactggt catctgacaa ttgacttttt tgtcaataca
      781 atcagtgtat tggggattat cttaccgttg tattacttta tcaaaatgct gacttcaaaa
      841 gatgtaactg ctgaagaaaa accaagagta tgggcttata tcccattatt ccttgcagca
      901 atcattttct ggtcattaca agaacaaggt tcatctatct tagctttatt tgctagtgaa
      961 cgtacacaat ctacgctatt tggtgtaacg atccaagcaa gctggtatca atcattgaat
     1021 ccaattttca tctttattct gacaccagta ttcgtaacga tgtggacgaa acttggtgaa
     1081 cgtcaaccat ctacagtagt gaaattctct cttggacttt tatttgcagg attatcgtac
     1141 ttgctattga tgttgccggg tatgctttat ggtacaagtg gaaaagtaag tccattgtgg
     1201 ttgttgggaa gtttcttcat cgtggttatt gctgaattgt gtctttcacc agttggtttg
     1261 tctgtgacaa caaaattagc accacgagca tttgaatcac aaacaatcgc tatttggttc
     1321 ttagctgatg cttcttcaca agcaatcaat gcacaaattg cacgcttcta cacaccggga
     1381 acagaatctg cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg tatcttgcta
     1441 tttactgtga aagaaccaat caaaaaacta atgggctcta ttcattaatc aatgaacata
     1501 aaaacaggaa ttgcttattt gcagcagttc ctg
//
LOCUS       DAZAID010000200         1492 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000200 DAZAID010000000
VERSION     DAZAID010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.206"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG9BE_002941"
     CDS             131..505
                     /locus_tag="ACG9BE_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN3033617.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG9BE_002942"
     CDS             complement(619..1248)
                     /locus_tag="ACG9BE_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN3033618.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG9BE_002943"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG9BE_002943"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          507 a          226 c          261 g          498 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aa
//
LOCUS       DAZAID010000201         1483 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000201 DAZAID010000000
VERSION     DAZAID010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.207"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACG9BE_002944"
     CDS             64..972
                     /locus_tag="ACG9BE_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFN3033619.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1483
                     /locus_tag="ACG9BE_002945"
     CDS             972..>1483
                     /locus_tag="ACG9BE_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033620.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGV"
BASE COUNT          511 a          216 c          345 g          411 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgt
//
LOCUS       DAZAID010000202         1480 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000202 DAZAID010000000
VERSION     DAZAID010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.208"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACG9BE_002946"
     CDS             64..972
                     /locus_tag="ACG9BE_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFN3033621.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="ACG9BE_002947"
     CDS             972..>1480
                     /locus_tag="ACG9BE_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033622.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DAZAID010000203         1421 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000203 DAZAID010000000
VERSION     DAZAID010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.209"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACG9BE_002948"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACG9BE_002948"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFN3033623.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          496 a          184 c          236 g          505 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta
     1381 gagtaattaa ttagatgttt attttatagg actgatacta t
//
LOCUS       DAZAID010000204         1408 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000204 DAZAID010000000
VERSION     DAZAID010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.210"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..229
                     /locus_tag="ACG9BE_002949"
     CDS             <1..229
                     /locus_tag="ACG9BE_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFN3033624.1"
                     /translation="PEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDIDS
                     EEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            234..1181
                     /locus_tag="ACG9BE_002950"
     CDS             234..1181
                     /locus_tag="ACG9BE_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFN3033625.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          462 a          197 c          292 g          457 t
ORIGIN      
        1 acctgaagtt tttgaaatga atgcctcaat gtattctttt aaacctgaat ttctaaaaaa
       61 aagtaattat atttttgatg ggcaatgtgg gataataaaa atgagagatt atctaatttt
      121 agatattgat agtgaagaag attttaaatg gttagaattt ttgtttccta aatttttaga
      181 agattcttcg gagcttcaag atatctataa ttatttatat cggaggtaaa aggatgagct
      241 gttatcttat caccggtggg gctggcttca ttggttctac cttagccaat catttaggag
      301 aagaacatgt ggtaatcgtc gtcgatgact tatcaatggg aaaagtagaa aatcttgata
      361 tgacgaaaaa tatcactttt attgagggag atgtcgctga gaaatccttg atggaagaga
      421 tcatgaaagc taataaattt gattatatct ttcatttagc agcagttgct agtgtggcag
      481 attcagttgc ccgaccagta gaaacacatc gtgtgaattt tgaaagtgta ttaatgattt
      541 tagaattgat ccgcaagtat caacctgatt taaaacggat cgtgtttagt tcttcagcgg
      601 ctgtttatgg ggatgaaccg actttaccaa aaaaagaaga gtccgtgatt cgcccattga
      661 cgccttatgc ggttgataaa ttcgctgcag aacaatatgt gctagattat tgtcatttat
      721 atgatgtacc cggtagcgca gttcgttttt tcaatgtcta tggtccaaat caaaatccga
      781 attctccata cagtggtgtg atttctattc tcgtagatcg ctataagaaa caaatcgtag
      841 gagaagcaac ttcatttaca ttatttggtg atggaagcca gtctagagac tttgtttata
      901 tagatgatgt aatccaagca ttacttttag ttgcgaatga ggaagctgca ctggggaaac
      961 aattcaatgt tggaacgggt aaagcaacca cacttttgga attgattcat gcaatcaatg
     1021 aaattctcgg tgtagaattg acacttgaat acaaagaaga gcgttcgggt gatatccatg
     1081 actctctagc agatattact aagatccagt caattggcta tcaaccgaaa tatggtattt
     1141 taagtggaat gactaattat ttgaaaactg aaatcaatta aaacatacta agataagatt
     1201 agagggtaaa ttcaggctct ttgtcaataa ggactgatga gttttgtaaa atcaaatctg
     1261 ggatagaagg gctctttgtc aaatgatgtt ggtaaagaga aatcttagaa taatttggac
     1321 agaacagaaa gaagaaaacc tctttctgtt ctgtttattt ggttaaaaat gttttgccat
     1381 gacgaatcat aattcgtagt ttgaaatt
//
LOCUS       DAZAID010000205         1389 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000205 DAZAID010000000
VERSION     DAZAID010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.211"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG9BE_002951"
     CDS             <1..969
                     /locus_tag="ACG9BE_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFN3033626.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="ACG9BE_002952"
     tRNA            1159..1232
                     /locus_tag="ACG9BE_002952"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          452 a          245 c          278 g          414 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatat
//
LOCUS       DAZAID010000206         1368 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000206 DAZAID010000000
VERSION     DAZAID010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.212"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            384..1058
                     /locus_tag="ACG9BE_002953"
                     /pseudo
     CDS             384..1058
                     /locus_tag="ACG9BE_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1051..1212
                     /locus_tag="ACG9BE_002954"
     CDS             1051..1212
                     /locus_tag="ACG9BE_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033627.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          528 a          196 c          242 g          402 t
ORIGIN      
        1 gaactaaata aaaatgacag gtaatcattt ttgtttacca gcattataac attttgcata
       61 catggatgac aaaagaacca attcccactt gcttgagtac aatcttaaaa gaaactggaa
      121 ggcaaaaagt aattatagaa acgaaaaata aatcatatcg ggcactcaga catgaaaaca
      181 accctagaaa tatataacta gtccaatgaa aaaaaagcga atgccgtaga aaacaaggat
      241 tctgcgtaca ttcatatctc attttgcgag catatctgct attactgtga ctaaatgtta
      301 atatggttaa aatgaccgtt atatttttta tataaatatc gtataattag tatgaatata
      361 gaagaaggaa agaaggtgca atcttggaac ctaaagacaa gtatttagat tacgctaggg
      421 tcaataaaga taaatttatt gaaacaatca tcaagggaaa aatccctgat gatgaaaaag
      481 atgcggtatt catggctgga agcccaggag caggtaaaac agaggttgca ctaggtttag
      541 cttaaaatta tgacaaccat gttgtgattg atgcggacta ttttagagca caatttccag
      601 aatacaatgg aaagaactca agtgtttttc agaaagcttc ttcttggtta gttgaacaat
      661 cattaaaata tgtactggaa catggctatt ctttcatact agaggatact tttgctatcc
      721 ttagtgcaga gaaaaatatt attcgagccc ttaaaaataa ctttcgcgtt acgattttct
      781 atgtttacca agatcccaaa gttgcctggg atttcactcg aaaacgtgaa ctagctgaag
      841 gacgtcatgt gccaaaagaa atgtttatca atgcgttttt taagtccaga gagaatattg
      901 aaaaagtcaa aaatagacat cctgaagtag tccttcacat aatgattaag gattatcaaa
      961 acgacatttc tgaagtgcat tatgcagcag ataatattca attagtacta ccaatacaat
     1021 atacttcaaa agagttggag gaggaattac atgactgaga aagaacaagt acaacaaatc
     1081 gtgaagaagt ataataaaag tatcgctgat ttatcagaaa acgcctcagc gaaagaattt
     1141 aaaacagtaa tgaattacgt agcagatgaa gcaaatcgta agcagcgaaa actagttggg
     1201 ttggataaat aaatttttta aaccatatcc ttgtaaagtt gagtttttta ttttaatgaa
     1261 ttaagactat taaatagata attattgtaa cttaaaaaac acagctaaaa attagttgtg
     1321 ttttttatta ggtttacttt tctggtataa aaggaactag ctactttt
//
LOCUS       DAZAID010000207         1368 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000207 DAZAID010000000
VERSION     DAZAID010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.213"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..936
                     /locus_tag="ACG9BE_002955"
     CDS             <1..936
                     /locus_tag="ACG9BE_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFN3033628.1"
                     /translation="SLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKG
                     LLNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKIT
                     EEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKE
                     LYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLE
                     PMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSL
                     DEIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1008..>1368
                     /locus_tag="ACG9BE_002956"
     CDS             1008..>1368
                     /locus_tag="ACG9BE_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFN3033629.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYY"
BASE COUNT          597 a          159 c          219 g          393 t
ORIGIN      
        1 tctttgttaa aattaaaaga aaaggaatat attattgacg atgctgtttc tgtccctctt
       61 attttaaata aacgagagaa aaataattta aagaaaaaaa tgctcattcc atttgattca
      121 gaagtgaaag gattactcaa taaactaact atttctaata tagactcgac tagggaaaaa
      181 atcataaaaa tatttgataa aattaataat ttattcaaga atgacaatgc tgaattaagg
      241 aagggtcttt tagagtttaa acttcaagaa ctagaacttc attactctta tttagtgaaa
      301 attaccgaag aaaaagaaca acaaaaagca ataaaagaac agatgataga agaggaaaaa
      361 gttcgtagag aaattgatcg tgaaaagaaa cgtattgata aagaacaacg tcaattcaat
      421 tctgaaattt ccaaattaat cgcatatatg caaaaagcta atgcagatgt ggaaaaagaa
      481 ttatatgcaa ataaaattca agaactggaa gaaaaattaa aagaattaga agttatacgt
      541 gaaaatgtat tacaaagaga attaaatact agagccggtt atgtgtatgt aattagtaat
      601 attgggtctt ttggtgaaga catatataaa attggtatga ctagaagatt agaacctatg
      661 gatagaataa aagaattgag tagtgcatcg gttccatttg aatttgacgt tcatgcgatg
      721 attttttcag aggacgctcc gtctttggaa actaaattgc ataatcattt tagaaaacaa
      781 gaagttaata aaattaatca aagaaaagaa tttttcaaag tttcactaga cgaaatagag
      841 aaagttgttt tagaaaatta taatggaaca gtaacattta caaaattagc taaagctgaa
      901 caatatagac gatctttaga attgtcaaaa gattaaatat ataaaaagcc ttcgggcttt
      961 ttcttttaac aattaatagg aacgtatatt cgaaaggaat agaaataatg tggttagaaa
     1021 aaataaatga caaaaaattt aagtacaccg aacgttatac ggatccctta actgaaaaaa
     1081 agagaaaagt gtccgttact ttatctagca attcacgcca agcttggaat caagctaact
     1141 tacttttgaa tgaaaaaatc gcagaaaaaa taaaacgaaa tgaagaatta ccactaactt
     1201 ttggagaatt aaaaactaaa tgggatgaaa aatacaagcc aactgtaaaa gaaagttcct
     1261 atcgaacaac tcaggtttat ctatcattaa tttcaaaata tataaaagat gatgtacttg
     1321 taaaaaacgt taatagtaat cttatacaag atatgctaga ttactact
//
LOCUS       DAZAID010000208         1355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000208 DAZAID010000000
VERSION     DAZAID010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.214"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACG9BE_002957"
     rRNA            <1..1355
                     /locus_tag="ACG9BE_002957"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAZAID010000209         1314 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000209 DAZAID010000000
VERSION     DAZAID010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.215"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(90..163)
                     /locus_tag="ACG9BE_002958"
     tRNA            complement(90..163)
                     /locus_tag="ACG9BE_002958"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(127..129),aa:Ile,seq:cat)
     gene            complement(352..>1314)
                     /locus_tag="ACG9BE_002959"
     CDS             complement(352..>1314)
                     /locus_tag="ACG9BE_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN3033630.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          266 c          248 g          432 t
ORIGIN      
        1 ctcatttgag tggcaatgaa gaaaagcttt agcttgtata atactcttca aaaaattcta
       61 acacagaacg attcaaatgg ctacgttaat gtaccctgta ggactcgaac ctacgaccgg
      121 acggttatga gccgtctgct ctgaccaact gagctaaggg tactggttgt tgccacataa
      181 agccataaac aatcaaccag tagaatgtgt ggcaacaaac ctgttatcgc atagcttgga
      241 gtgtgactat tatgggtgat agtgaagata tgcgataaca tcactatttt atcgaatgat
      301 ttttatagtt gtcaatatag ttatgtaatg ctcctcaacg aggagctatt tttatcgttt
      361 aggaatattt aaataccaac gtttgtcatg gaaatcttgc gcaccgcctt tagtgtttcc
      421 ctctggatca ttcgttgccc gcatcatgac atagactttc ttattaggga agttacgcat
      481 gttaaaagat acatgatagc caacatttcc agaagtatta taagcttgat ttacatctgc
      541 tctataaatt ccatcagctc ttactcgagc taattctttc ccagtattgt aatccataat
      601 gaagatatac tcgtatttat agttagcaat gtgccatcca gctacatgca aatttgcatt
      661 ttcgatttct ccaaactgat caatgtgggc gtaattcgtt ccatctgtca gtgtaggatt
      721 agctgcacca gctcgtgttg gatcaatgac aggcttgttt tcagaagttg ttggattttc
      781 atcggtaaat ccatgagcta aatcatatgc taatttttct ttacttacgc ccatttcaga
      841 aagataaccg taaggatctg tatgatcgcc ccagatgttt tgtgttaccc ataaatgcga
      901 tttgattccc ggttggttat aaggagtgtc caacgttaat ggaataccat atttcattgc
      961 tgaatctcta gccaattcaa cgtatgcctt gtagtttttc tcaaacgttg ctttatcatg
     1021 tgtgtgttgt aactcaatct gcacaggact gttggcatta gcatacgaac cagcaccgta
     1081 ctgtacataa ccaggttgac cgacttgata aacaattccg ccgtctccca caatataagc
     1141 agtataagcg ctagtccatg aacgttgcat atactgcgct tcattgcgtc ctgttgctgt
     1201 ttcattagcc gtttcatgca gtaaaatata ctgattattt gctacttgtg agctaccttc
     1261 atttgcgccc aaattaaatt cattgttgat agtataggca aatccgttaa ttgg
//
LOCUS       DAZAID010000210         1284 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000210 DAZAID010000000
VERSION     DAZAID010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.216"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1284
                     /locus_tag="ACG9BE_002960"
     CDS             <1..>1284
                     /locus_tag="ACG9BE_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFN3033631.1"
                     /translation="NDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRAC
                     RSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQS
                     YFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKK
                     ILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEV
                     EFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKSQ
                     AEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYP
                     FFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNGK
                     IEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          431 a          250 c          246 g          357 t
ORIGIN      
        1 tgaatgattc tatcaaaaaa atgctgagaa taacagagaa agatttgatg attacagagg
       61 tctcttacga gacccttcag aagaaaaaga cgttggtcgt cgatgctgtt ctctcgccta
      121 ctcctcgtgc ttgtagaagt tgtggttcta ctgtggtaga tggaaacggg aaagcaatta
      181 tagtgaaaaa tggaaaaaag gaaacgattg tccgttttga acaatacaat catatgcctt
      241 tggttatgcg cctaaaaaag caacgctata cctgtaaaaa ctgtcgcacc cattggacgg
      301 ctcaaagtta ttttgtccaa cccagacatt caatcgcaaa tcatgttaga tataaaattg
      361 cttctttact gactgaaaaa gtatctttat cttttattgc gaaaagctgt caggtatctt
      421 tgaccaccgt tattcgtaca ttgaaagagt ttaaaagcta tttaccaaag caatctaaga
      481 agatcctccc cagagtattg atggttgacg aatttcgttc gcatgcttcc atagaagata
      541 agatgagctt tatttgcgcg gatggcgaaa caggaaaatt aatagatgtt ttgcctacgc
      601 gtaaattacc tcgattaaca agctatttct taggttgtac caatccagaa gaagtagaat
      661 tcttggtgac agacatgaac gccgcctact tccagctcac caaacgtgtt ctgccaaatg
      721 cgaaagtcgt gattgatcgg tttcatattg tcaaacacat gaatcaagcg ttcaatgagt
      781 tgcgtatccg tgaaatgaat gaacttcgta aagccggaca gaaaagccag gcagaaaaac
      841 tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa catcaaccat tatgaataca
      901 aaacatggaa aagtttccga gcaccaaaat acccatttct tactgaagca atgatgattg
      961 atcgattgct tgagttttct ccgcccctaa aggaggcgta tccctttttt catgaattag
     1021 ttgaagcctt tcgagacaaa gaccctgact tatttttctc cttattggca gaacttcccg
     1081 aaacgttaga tgacagcttt cgggaaaagc ttcaaaacct tctgacctat gaagaaggca
     1141 tcaccaacgc aatgatctat ccttattcca atggaaaaat agaagcgaag aatacccaca
     1201 taaagacaat gaaacgagta tcctacggat ttaaatcatt tgagaacatg agaattagaa
     1261 tctttttgat caatcaatta atca
//
LOCUS       DAZAID010000211         1255 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000211 DAZAID010000000
VERSION     DAZAID010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.217"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..511
                     /locus_tag="ACG9BE_002961"
     CDS             <1..511
                     /locus_tag="ACG9BE_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033632.1"
                     /translation="EIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSILTLAMAGCS
                     DSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRS
                     NRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDED
                     NPKTYDILA"
     gene            complement(572..937)
                     /locus_tag="ACG9BE_002962"
     CDS             complement(572..937)
                     /locus_tag="ACG9BE_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFN3033633.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1013..>1255)
                     /locus_tag="ACG9BE_002963"
     CDS             complement(1013..>1255)
                     /locus_tag="ACG9BE_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3033634.1"
                     /translation="SIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKYE
                     EGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
BASE COUNT          428 a          218 c          245 g          364 t
ORIGIN      
        1 agaaattatt aattttcgtc tgaaaacaaa aaaagaaagc gttattattt tagtctattt
       61 tcaggggggg aaccttatga taaaaaaact atttttacta tcaatattaa cgctagcaat
      121 ggcaggttgt tctgactctg gcttagcaac tgaactgatc acaaaatcaa ctgaagccac
      181 gacagaggag ttaaaatgtg gaaacgatca cagtgactac agaggcttaa atcgacgatg
      241 catgggaata ttaggcagaa gcaattgtaa gcatcgatgt agtaatcgaa gaaggtgtag
      301 aagaagcaac agaaaaatca aaagaaagat caaaagaagc aatctgtgct gtgcatcgta
      361 tacagacgtc gaagatgcgg tacttttaaa ccacgtcagg agatgttcat ttatgtctag
      421 ggaagttggc gatgaaccaa aactagagta cgcttttagt tttgcacgtg aggatgaaga
      481 taaccctaaa acttacgaca ttttagcttg atctaagatg gttaaagctt tttaatgaag
      541 taagaggaga tagaaggctt ttctagttat attaggtaag ataaattcct ttttgctcca
      601 aatttgctaa acattctgct agataaactt catcgtgttc cggataaatc ggcacgttgc
      661 gctctacagt aggtaaatca aaaaatgctt cgcatgattt gccacgatac ataggatttt
      721 tccactctgg tgctgggctg taaccacgtt tttccatttc atccatgaca agctgatgat
      781 attggaataa tctataagga gaatagtgaa aaacataatc cacagtagca tgtttctttc
      841 cccagccatt tcctcttaat gcacagcatt cccgatgttg tcctaaaagc tgttgacgag
      901 gaagctttga aatcaatttt tcatgccata atcgcataga aatgtctcct ttatatatag
      961 attgttaacc ttagacgtta aatgaaaaaa ccagcaccaa ggctggaaaa aatcacttca
     1021 gtttgcttgc tgtttctgag cgaccaatta ggtaatcaac ggaaatatcg taaaagtcag
     1081 cgatatagat taattcaatg atgcttggaa tttcttctcc ttcttcgtac ttacgatatt
     1141 tgaataaagg aatacttaac gcgctagaaa cagctacttg agataaagat cggccagttc
     1201 ttaagaaatt cagacgacaa cgtaaagtat ccagtaaaat agcttcttcg atact
//
LOCUS       DAZAID010000212         1246 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000212 DAZAID010000000
VERSION     DAZAID010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.218"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG9BE_002964"
     CDS             200..742
                     /locus_tag="ACG9BE_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033635.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACG9BE_002965"
     CDS             898..>1246
                     /locus_tag="ACG9BE_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFN3033636.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAZAID010000213         1195 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000213 DAZAID010000000
VERSION     DAZAID010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.219"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG9BE_002966"
     CDS             <1..1076
                     /locus_tag="ACG9BE_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN3033637.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAZAID010000214         1193 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000214 DAZAID010000000
VERSION     DAZAID010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.220"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..330
                     /locus_tag="ACG9BE_002967"
     CDS             55..330
                     /locus_tag="ACG9BE_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033638.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            757..>1193
                     /locus_tag="ACG9BE_002968"
     CDS             757..>1193
                     /locus_tag="ACG9BE_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN3033639.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDE"
BASE COUNT          451 a          205 c          181 g          356 t
ORIGIN      
        1 ataaccaaag gcgctatgat ttccatatga aaaaaataaa gtcaggtgaa aaaaatgaaa
       61 cttgaggata aaaaagtcat tttgaagaaa atcaaacgac taccagacca aatagatggc
      121 tatacgatct gttctttcaa aaaagacagg agtataaaag cttatacgca aaacacagaa
      181 aaaggaataa tctatttcct acatgaagaa ggttacgata cccaaacctt tatgtttact
      241 gaaaaaaaag aatttcataa acagatgaaa aaactgattg aaaaagaatt tccaagaagt
      301 cataaactct atgtgaatca gcaattttaa cttctaactg ttctttatta attaaaaatg
      361 ttctgtaata aaataaaact ttataacaac tataacaaaa ccgaatgcaa aacagaagtt
      421 ctataagtta taatcatagt tgttaggtga ttcatctagt aaaaacacca gatttcaaat
      481 ttcacacaat ttaaactaga tcaatcatca accttcttca aattgttaga accctttccc
      541 gaggagctgc agcataagca aaaaaacaaa caggaagcca aatggctaag acattttttc
      601 tattagtcta gttgcttctc gtcgcaagtt taagacttta gaaagttatt tctaatgatg
      661 tttgggttgt tttgatcaat gcttatgcac agctccttac ctattattta tctcaacaga
      721 tctaacaaat taatggacta tttaaggagt ttttttatgc ttacgatcta ttcttctcgc
      781 acacatagtt accatctgat ggcaaactgg ctgaaagacc atcaaatgcc ttacagagaa
      841 cgaattttca cacctaaaca gcctttaaca aggaaagaga tattttattt attgtcatta
      901 acagaaaacg gatttgacga tctattagct aaacgctcga ttgattataa aaatttgaaa
      961 gaccaaatcg accaatgtac gacctcagag cttgtcgatc tgatcgtctc gcattttact
     1021 ttgttacaac gtcccatcat tactgacgga aagaaattat tagtcggatt caatgaagat
     1081 agcatgcgtc tttttatccc tgtacaatgg cgcagaagtc aactgaaatc tgtctataac
     1141 gattttacat gggtaagcaa tctagaaaat tctacggatt tagatgatga att
//
LOCUS       DAZAID010000215         1140 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000215 DAZAID010000000
VERSION     DAZAID010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.221"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..479
                     /locus_tag="ACG9BE_002969"
     CDS             <1..479
                     /locus_tag="ACG9BE_002969"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033640.1"
                     /translation="EDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFN
                     SNQSVLLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIA
                     EVIMVLSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            586..>1140
                     /gene="mef(H)"
                     /locus_tag="ACG9BE_002970"
                     /gene_synonym="mefH"
     CDS             586..>1140
                     /gene="mef(H)"
                     /locus_tag="ACG9BE_002970"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFN3033641.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIME"
BASE COUNT          383 a          158 c          195 g          404 t
ORIGIN      
        1 aggaagatat tttaaaagca atttttcaaa gaattggaaa tgaaaatgca gtaatatttg
       61 ctaaaattag agataacgat agtttaaacg gtttagaaaa gttaagagaa atatttaaga
      121 cagcggtatt caattctaac caaagcgttt tattgacagt ttctcctcaa ctattaaaca
      181 atcctcgttt tcttgctatg caaatagaac aaatgtatga aattgtagta cctgaatttg
      241 ttgaacccgt tttagaacaa ggtatcaagg ataagtcttt ggagattgaa aattcacatg
      301 aaattgcaga agttatcatg gtattaagta atgtatggct caacccatta gttgaaatga
      361 cggataaaga gggtatggaa aaacgttgca atacattcaa tgccctttta aatggaatag
      421 gcatagacac attattagac gaagaaacaa tttctgcatt tatacagttt tgtaaataac
      481 tgtatttgtt atccctatac gatagactgc tctataaaag agcagtttat ttaaaccatt
      541 atataaaccg acggtctgtt tgattaaata aaaaggagag attttatgca agcagaaaaa
      601 aaacttttta atcgtgattt tatacttgtt gttataggac agataatttc tttatttgga
      661 aacgctatcc tacgatttgc attaccgtac tatttgttaa aagaaactgg ttcatcagct
      721 ttgtttggta ttgctacagc ctgctctttt ttacctatgg ttattttgtc gctggtaggt
      781 ggtattattg ctgacaggac aaataaaaga aacattatgg tcatactaga ttttgtaaca
      841 gcaatcataa ttttagtatt ttatttgtca ttgggaaaaa ttcctatcat acctttgttt
      901 attgttgctt tgatgttact ttatggtatt tctggtgcat atcaaccagc ggtacaagca
      961 agtattcctc tattggtatc atcagataaa cttaccgtcg gtaatgctat aataaatcaa
     1021 gtaaatactt tagcaaactt tatcggacca gctataagcg gagtagtttt tcaattttat
     1081 ggtatcaatc ccattttaat tgttagtatt atatgtttta ctttttcagc cattatggaa
//
LOCUS       DAZAID010000216         1001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000216 DAZAID010000000
VERSION     DAZAID010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.222"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1001
                     /locus_tag="ACG9BE_002971"
     rRNA            <1..>1001
                     /locus_tag="ACG9BE_002971"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          310 a          198 c          289 g          204 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt g
//
LOCUS       DAZAID010000217         1001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000217 DAZAID010000000
VERSION     DAZAID010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.223"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1001
                     /locus_tag="ACG9BE_002972"
                     /pseudo
     CDS             370..1001
                     /locus_tag="ACG9BE_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta c
//
LOCUS       DAZAID010000218          987 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000218 DAZAID010000000
VERSION     DAZAID010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.224"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>987
                     /locus_tag="ACG9BE_002973"
     CDS             <1..>987
                     /locus_tag="ACG9BE_002973"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN3033642.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKMF"
BASE COUNT          375 a          159 c          189 g          264 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgttt
//
LOCUS       DAZAID010000219          950 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000219 DAZAID010000000
VERSION     DAZAID010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.225"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            188..511
                     /locus_tag="ACG9BE_002974"
     CDS             188..511
                     /locus_tag="ACG9BE_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033643.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            675..929
                     /locus_tag="ACG9BE_002975"
     CDS             675..929
                     /locus_tag="ACG9BE_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033644.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
BASE COUNT          259 a          139 c          146 g          406 t
ORIGIN      
        1 catcataaat aaaatttatt tttgttactt ttcaactata gcacgaatga caaactctca
       61 caagtttcaa aatataagta aaataatatt gttattctta tttcttttac ttgtgtcata
      121 actattttgt tactttaatt tttatttgct tattttagaa aaaatgttat tcttttattt
      181 attctcaatg ttaggagtga ttattttgcc atttattgcg attttattcg atttagttgc
      241 ggcagcagct tattttctcc aattaaatta tcaaacctca ggaattctgt ttattggaat
      301 gatttttcaa ggtgtgatta cccttttgct tcttgtcatg atgctcagct ataaaggaaa
      361 aagatatgcc cgtattcaaa caaaagtatt tgttaaatat gtttccattc gctatgggat
      421 catcattctt tcctttttag tgaatgcgat cgtcctcttt ttatatgtat tgaattactt
      481 agatatcaat ccattgattt tttctcgata gataagacac attcagcgga acagttcctg
      541 cttatcagca gaaaactgtt ctgctttttc atttttcatt gatctattct tcttttattt
      601 tcctagcatt tcatttgtga tgtatgcaat aacatgttat cttttattat atgaatcgtt
      661 gaggagtccg ttacatgatt acgttggtga gagttttgtt ttgggtgcct gcggtcgcac
      721 tggtcgcaag tattgtttat cttatgaatt ggaacaaaga acggttttat ttagctattt
      781 tgactcttcc ggctatctac tttatgtgga aagtttttaa ttacaactac tttgaacccg
      841 atagtgtttt tatagaagaa ttatcaggac tggttttgtc tttattgatc gtcattcttt
      901 atttgatacg tttaaataaa aaacattgag tactattgat tgcatgtgta
//
LOCUS       DAZAID010000220          920 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000220 DAZAID010000000
VERSION     DAZAID010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.227"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>920
                     /locus_tag="ACG9BE_002976"
     CDS             <1..>920
                     /locus_tag="ACG9BE_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN3033645.1"
                     /translation="KSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQ
                     KNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALL
                     DIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIV
                     QHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASF
                     KDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYT
                     TTLRTFKKFLPQIQNALHYSYSNGPLECLN"
BASE COUNT          279 a          186 c          183 g          272 t
ORIGIN      
        1 taagtcttgc caaagaacat tcgtagcgga tacctctgtt gctgaaaaac actgtttcat
       61 tagtcaaaaa gttcgttggt ctgtcattgc tcgcttaaaa gagaatactt ccatgacgga
      121 aatcgctcgt cagaaaaata tttccacttc ttcggtctat cgtgtcatga agcgattcta
      181 ccgcccattg aatcctttca aacagacttt gccaaaggtc ttatgctttg atgaattcaa
      241 atcggttcga agtgttgcca gtgcaatgag ttttatcatg atggacggac aaagccatgc
      301 attattggat attgtcgaaa atcgacgctt gccttattta gaacgctatt tttctcggtt
      361 ttcattagcg actcgtgaag ccgttcaatt gattgttatt gatatgtatg cgccttatgt
      421 ttcgttggtg aaaaagcttt tcccgaatgc tcagttgatc atcgatcgtt tccacatcgt
      481 ccaacatatt ggcagaactt tcttgaatca tcgagtgaaa gaaaccacgg tacgacttaa
      541 aggaaattcc cactctcaac gagggttagg gaaaaaattg aagcgctatt ggaaactcct
      601 acaaaaagaa gaagcaaagt taaactatga aaaacgggtt tggcgtgcca gttttaaaga
      661 ttatttgact gaaagtgaga ttgccgatcg actgttagct gcttgtccca acttacgcca
      721 aggctatcag ttataccaag acattcttta tgcggtaaag aaaagagatc agtcactatt
      781 tgaggattgt ttaactagag acattagcgg tctgccagaa acctatacaa ccactttacg
      841 aacgtttaaa aagtttctgc cacagatcca aaatgcgctt cactacagct attcaaacgg
      901 accattggaa tgcttaaaca
//
LOCUS       DAZAID010000221          906 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000221 DAZAID010000000
VERSION     DAZAID010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.228"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..767
                     /locus_tag="ACG9BE_002977"
     CDS             <1..767
                     /locus_tag="ACG9BE_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFN3033646.1"
                     /translation="IKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKYYISLLCKEE
                     IIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKN
                     KGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGML
                     RNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLE
                     IREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          335 a          144 c          164 g          263 t
ORIGIN      
        1 tcatcaaagt tcctaaattg aagtcattag taagaattaa gcttcataga caaccaaaag
       61 ggattatcaa atctgctaca atatcacgtc attctagcgg taaatactat atttctttgt
      121 tgtgtaaaga agaaattatc gaattaccta aaactaattc cacaattggt attgacttag
      181 gtattatgga ctttgcgatt ctttctgatg gacaaaaaat tgataataat aaattcacgt
      241 ccaaaatgga aaagaaacta aagcgtgaac aacgcaaatt gtctaggcgt gctttattgg
      301 ctaaaaataa aggtaccaat ctatttgaag ctaaaaatta tcaaaaacaa aaacgcaaag
      361 tagctagatt gcatgaaaaa gtaatgaatc aacgtactga ttttctgaat aagttgagta
      421 cagaaatgat caaaaatcac gatattatct gtattgaaga cttaaacaca aaaggtatgt
      481 tgcgaaatca taaattagct aaaagtattt ctgatgtttc ttggtcaaag tttgtgacta
      541 aattacaata caaggctgac tggtatggac gaaaaatcat caaagtagat aaatggtttc
      601 cgtctagtca gatttgttca gagtgcggac ataaagatgg caagaaatct cttgaaattc
      661 gagaatggac ttgtcctgtt tgtcatactc atcatgatcg tgatatcaat gctagtatca
      721 atattctgac cgaaggtcta agactacatt ctatgggatt ggcttagatc caatcatcaa
      781 gaaccgtagg aactacgggg atagcttggt aaataagaga caccgctgtt agtaaagaaa
      841 tacgctatca agtatgctct attcccaaga agctcccact tcaagcgtta agaaccgtca
      901 ggttta
//
LOCUS       DAZAID010000222          890 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000222 DAZAID010000000
VERSION     DAZAID010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.229"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACG9BE_002978"
     CDS             <1..888
                     /locus_tag="ACG9BE_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFN3033647.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAZAID010000223          887 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000223 DAZAID010000000
VERSION     DAZAID010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.230"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..830
                     /locus_tag="ACG9BE_002979"
     CDS             90..830
                     /locus_tag="ACG9BE_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033648.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          326 a          162 c          183 g          216 t
ORIGIN      
        1 tttcttaaaa taatcagcgc attgctactg aaaacaatga atctgtgcta ttattgagaa
       61 aagttccatt tttgagaagg atggtttgaa tgaaagaaat cagacgaaaa catttccgtt
      121 ttcctgcgta cgatgatgag ttaggcgtaa aattaaccaa cgacaagaag cgagtattat
      181 ttgacggaca agaagatctt ttgactgaac agaatcagtc cccgtttgaa cagatgaaag
      241 aatttcattt cacgaacacg aacaccgaga aaatccgtcg caagcgtcgt gcaacaaatg
      301 gattgaagtc agctccgcgt aaggaaaatt tggaaaagca taaaggaaaa ttaccggatt
      361 ataccggcca ttaccaatca gaaatcacat cgacaggacg aaaaaaactt tttggtgatt
      421 cctctcgttc tgtcagacaa agtgaaacaa ggacagagtc caagcatact acacgaaacg
      481 catctgttga cacgaaggtg cgggagcgtt cttattttgt tcctaaatat gtacccgcat
      541 cgattatccc agaccctaga gaaagcaaaa tttctgagga tcatttaatc gaatcgatga
      601 aaaaaacgga tgactcttat ctgttgttcg atacagaacc gacgccgtat caagaaaaaa
      661 gaaatggtga gccgtctgta cgaacattca atcgaacaaa aacgttagag gaaacaaagc
      721 agtcagaaga aaaaaaatcg aaaaaaagcc atggtatctt agaacgctca ttacaaggat
      781 tgatcgatga tcaaggaaac tctttgggag aaaatagcta ttttaaataa gtgatataca
      841 aatttatatt cggctacagt tggaaaggaa acgctgcggc gtttcct
//
LOCUS       DAZAID010000224          843 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000224 DAZAID010000000
VERSION     DAZAID010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.232"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>843)
                     /locus_tag="ACG9BE_002980"
     rRNA            complement(<1..>843)
                     /locus_tag="ACG9BE_002980"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          184 a          259 c          187 g          213 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gct
//
LOCUS       DAZAID010000225          839 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000225 DAZAID010000000
VERSION     DAZAID010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.233"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..476)
                     /locus_tag="ACG9BE_002981"
     CDS             complement(<1..476)
                     /locus_tag="ACG9BE_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFN3033649.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLS"
BASE COUNT          306 a          129 c           91 g          313 t
ORIGIN      
        1 ttacttagat tttctgtata ttcaattaac ggttcatgga atagatcttc tatttttcca
       61 gtaaaaaaat aatgaactaa aatcagaata tctcgaatgt ctcctaaaga atacaaagtc
      121 aaattttgca atgaattata gagtcgttct ctatcagcca ttgcaacatt caaattgttc
      181 atttttagtt taaaaaaaac tttatcaata ttatttgctc taaatggacc aaataagtaa
      241 tatctggttt taaacatgta tagaagaaac atctcattaa aatcaccagt aaagaaatgg
      301 aaatctttag tttttttaat attatcagtt agtaactttt tataatcata aaaaaaataa
      361 gtagttctat ctgagcgata ctcttttata atactgaatt cggaattaaa aaccacgata
      421 ggtaacttta aacaaacatg aagagacttt aataagactt ctttatctga aaacactttt
      481 gtttctctct ttcctttcta catcattaaa tagtatttta tacgttatct aaaatactat
      541 tcctctgtca aaataagcta acttacttca ttataaaata tccacttatc gctttcatgt
      601 atattccacc gtaaaaagta cattttatca ccgagaactc atcatttttc atcatgatga
      661 atactattta catatatcta gttcaaagac aataattatt atgatccgtt attatctaag
      721 cactatacag ataataggaa tttttcgttt ctaaatattc caatttggga atccaataaa
      781 aattttcgtt caaatacgtg tctattcgaa ctcttttcta gttgttaatt atgaagggg
//
LOCUS       DAZAID010000226          824 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000226 DAZAID010000000
VERSION     DAZAID010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.234"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(357..470)
                     /locus_tag="ACG9BE_002982"
                     /pseudo
     CDS             complement(357..470)
                     /locus_tag="ACG9BE_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          295 a          129 c          131 g          269 t
ORIGIN      
        1 attgaaacat ccatctggaa tttgtctttt tcgttgcttt gctcataaaa atctgcatat
       61 tcatcactaa tattttttct aataaattct tccatcataa agatcacctc gaaaagagta
      121 tacgaacaaa cgttcttttt gtaaaggtgg aatttattat actaaataaa taggtgaaca
      181 agtacttgtg ccaaattatg tgccaaaaaa aatcgaattt aataaaacac aaacaaaaag
      241 aaatcctatt atgataacat ttcttataat aacaaacaca acagaagacg tgtaatagtt
      301 agtcaggaac gtacaaataa cccctgtatc ctttgcggta cagggggtta ttttgtataa
      361 gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct aagtatattt
      421 actccttgat aaagtaaaat agatgcatgc gtatgtctta gtccatcaat aaacagtcga
      481 agagaaaata gggaaaaatt gattactaaa gaaaaactta ctaggtcgac ggtgtagttg
      541 attttcatac ttcaatagat tcgaattaaa cgatttcgat tggcttatac cagtcctatc
      601 caacgaaaga tatatcgttc ttaactctag cggtataact tgaagcttta cgtaaagcca
      661 atgggaatga actggagatg actttttatt atttttagta atagctgatg ggttaatact
      721 tacaactttc cttttcaaag taccatataa aaaaacggtg gggttgttta gcgaaataaa
      781 ggatctaagc caatagctta gatcctttat tctatgcttt atat
//
LOCUS       DAZAID010000227          815 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000227 DAZAID010000000
VERSION     DAZAID010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.235"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..728
                     /locus_tag="ACG9BE_002983"
     CDS             66..728
                     /locus_tag="ACG9BE_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN3033650.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
BASE COUNT          271 a          151 c          157 g          236 t
ORIGIN      
        1 ccagattgtt tttcagacct cttctgttat actacgttca tagaaatgat tttggaggaa
       61 taaagatgaa aagcagcttg caatcacaag cttatcaagc gattcgtaaa caaattatct
      121 atgctgactt agaacctgga aggaaaatat ctgaaaaggc actggaggaa catcttgcta
      181 tcggccggac tcccattcgc gaagcattga ttcaactacg tcagcaagaa ctcgtgtata
      241 cgatacctca aagtggtacg tatgtttcca aaattaatct tgtttctgca gaaaatgctc
      301 gctttgtccg agaacagttg gaagagcaaa tcatgattga atgttgttcc aaattggaca
      361 cacaaacacg aatagtgcta gaaacgattt tagaagaaca agaaaaagca gtcaaaataa
      421 atgacgaacg cgcttttttc cgtgcagata atttatttca tgaagcttgt ttcgaaattg
      481 caggaagaaa agaagtttgg aactggttag atgaccataa tacccattta gaacgtttcc
      541 gctggttacg agtgaccatt caggggcttc catgggaaac gatcatggaa caacattatg
      601 atttactcaa ggctttagtt gataaaaaac cagaagaagc tcgatttcaa acgggattgc
      661 acttacacat gatgctgaaa gaacaggaaa ctgttactca acgttatccc gagtatttcc
      721 agcagtagca ttttcttgcg acctcttcta tttttataat taaccttcgt ttttgagtaa
      781 tctttattaa ataacaaaat atccattgac tttgc
//
LOCUS       DAZAID010000228          806 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000228 DAZAID010000000
VERSION     DAZAID010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.236"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..706
                     /locus_tag="ACG9BE_002984"
                     /pseudo
     CDS             99..706
                     /locus_tag="ACG9BE_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
BASE COUNT          291 a          127 c          138 g          250 t
ORIGIN      
        1 ttaagaattg ttgttttcgc tgtcgctatc caaatttaat gaatgtctgt atgttaaaac
       61 tgttgtcctt gtcatagata gtaaaggaga gcttgaaaat gaaaactgcg attacgtttc
      121 tattagagga atatgccgat tgggaaggtg cttatattac tagcacacta aaccaaaaag
      181 agggatggca aaacaaaact gcttctatcc aatcagaggt cacttccatt ggaggattaa
      241 aaacaaaaat cgattactta atcgaacaaa tacctgaaaa agttgacctt tttattatga
      301 tcggtggcaa ttcatgggac ataaaaaatc atcatttata tcaaataatc gataaattgt
      361 taaaaacaga cgtaccagta gctgctattt gtggggcagt tgattattta gctcagagcg
      421 gattattgca aggttacaaa cacaccggaa atgcgcaatt tccttggtta gaatcggaaa
      481 attatcagaa taaagaagat tttatagaac aacaagctgt gaaagatcgt aatttgatta
      541 ctgcgaatgg aacagctcct atagaattca cagacttgat tcttcaagca gtccattttg
      601 aaacagaaga aataagagaa aaaaagattc gtctatacaa acttggattc tatgattact
      661 ataaaaagta cggagatcct ttctcctaat cattctcttc aattaaatac tccttatcaa
      721 tagtatgaca agtaatttta ctgatggtta tttgtcacac tttttctgac atttatcaaa
      781 gaagatacac tctatttata ttcata
//
LOCUS       DAZAID010000229          805 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000229 DAZAID010000000
VERSION     DAZAID010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.237"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>805
                     /locus_tag="ACG9BE_002985"
     CDS             <1..>805
                     /locus_tag="ACG9BE_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033651.1"
                     /translation="FGFYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYPWLT
                     LYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSF
                     RSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAM
                     TMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPY
                     VDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLLICLIILTFNFKKLGIL"
BASE COUNT          252 a          112 c          117 g          324 t
ORIGIN      
        1 atttggcttt tatgatacgg catttcacat taatcgtgcg ttaagtttag aatctatttt
       61 tagctcccct attaattttg aaacgtttag atcatatggc atgcaagtaa ataattttta
      121 cccatggtta actctatatc ctttattttt attgatcaag tttactaatt tagcaatagg
      181 ctataacctt tttctttata ttgttacgct cattactcta tttatatgtc attatgtaat
      241 gtatgaaatt accaaaaaac atgttactag ctcctttttc gctattatat atacaacatc
      301 ttcttttagg agtgtagaaa tatttcttcg aggggcaatg ggggaattac tcgcaatgtc
      361 aatacttcct cttattttac tcgggtttat aaagctttac gactcaaaaa aagaatcttg
      421 ggttatgttg gcgattagca tgactttatt aatttacact catgttcttt ctgtagctat
      481 gactatggta atgattataa ttgctttaat tattcaattt tatcggaaaa agaaaataga
      541 aatcttctta attattaaat taataaaagc tgctatagtt actttacttc tttcactagc
      601 gtttataggt cctatgattg agcagacatt gtatcaagat ctgaatagac cttatgtgga
      661 tatattacaa aaaagagcaa tttatctggg taaagagttt ttgattggaa gtatagattc
      721 agaattacta tcatttggta ttggcttaac tttgcttatt tgtttaatta tactaacatt
      781 taactttaaa aaattaggaa tctta
//
LOCUS       DAZAID010000230          791 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000230 DAZAID010000000
VERSION     DAZAID010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.238"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..635)
                     /locus_tag="ACG9BE_002986"
     CDS             complement(39..635)
                     /locus_tag="ACG9BE_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN3033652.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          301 a          149 c          121 g          220 t
ORIGIN      
        1 aacattgtca atcaatgact aattttacaa aataaatatt atccaagaag accacttaga
       61 tattgacctc cataaatcaa ggccatacta tataagaaaa tcgacagtgg tttagcaatc
      121 aaaatgatta gtgtatatcg tttcactgac atgtttgtca atccagccat caaacataac
      181 gcatcgtcag gagcaactgg aagaaaaata gctagagcaa acagcttctc aaaacggttt
      241 tgattatcca gccagccaat atatttgtta tatgtcttat cgctaatcat ggaaagtatg
      301 aatggctttc cataacgacg ccctagcaga aaaatgataa tcgaaccaat gcagattcca
      361 atatagttat agataaatcc agcataaggg ccaaatatca ggacaccagc tgcagtactg
      421 atccctccag gaatgattgg aataactact tgcaggattt ggatgaagac aaaaataatc
      481 ggtcctaaaa tgatggaatc acctactaat cctcttaacg cattcaaatc tttgaaaaca
      541 cctaaattaa taaaataaat cgtcagcgcg attgataggc ctaatccgat aatggatatg
      601 aaatttatca gttttcgaga ggcagcaata ttcattattc atcaactcct tagaggttct
      661 ttgttaacta tactttacta gaaaagcaac taactgcgcc attttttgat atgacagaaa
      721 acaaacaatt ttgtaagttc atcataagta taaaagattg atctcaaaaa aacatccaac
      781 taaagactta t
//
LOCUS       DAZAID010000231          769 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000231 DAZAID010000000
VERSION     DAZAID010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.239"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..111)
                     /locus_tag="ACG9BE_002987"
     tRNA            complement(39..111)
                     /locus_tag="ACG9BE_002987"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(76..78),aa:His,seq:gtg)
     gene            complement(114..187)
                     /locus_tag="ACG9BE_002988"
     tRNA            complement(114..187)
                     /locus_tag="ACG9BE_002988"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(153..155),aa:Trp,seq:cca)
     gene            complement(194..274)
                     /locus_tag="ACG9BE_002989"
     tRNA            complement(194..274)
                     /locus_tag="ACG9BE_002989"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(238..240),aa:Tyr,seq:gta)
     gene            complement(276..351)
                     /locus_tag="ACG9BE_002990"
     tRNA            complement(276..351)
                     /locus_tag="ACG9BE_002990"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(316..318),aa:Phe,seq:gaa)
     gene            complement(387..459)
                     /locus_tag="ACG9BE_002991"
     tRNA            complement(387..459)
                     /locus_tag="ACG9BE_002991"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(469..541)
                     /locus_tag="ACG9BE_002992"
     tRNA            complement(469..541)
                     /locus_tag="ACG9BE_002992"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(506..508),aa:Val,seq:tac)
     gene            complement(557..628)
                     /locus_tag="ACG9BE_002993"
     tRNA            complement(557..628)
                     /locus_tag="ACG9BE_002993"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(593..595),aa:Glu,seq:ttc)
     gene            complement(643..732)
                     /locus_tag="ACG9BE_002994"
     tRNA            complement(643..732)
                     /locus_tag="ACG9BE_002994"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(696..698),aa:Ser,seq:gga)
BASE COUNT          210 a          212 c          177 g          170 t
ORIGIN      
        1 cttgccttac cgcttggcta taccccaaga ataaaaaggg cgaatgatgg gaatcgaacc
       61 cacgaatgcc ggagccacaa tccggtgcgt taaccacttc gccacattcg ccatggcagg
      121 ggcagcagga attgaaccca cactaacggt tttggagacc gctgttctac ctttaaacta
      181 tgcccctata aaatggagga gagtggattc gaaccactga accctaagga acggatttac
      241 agtccgtcgc gtttagccac ttcgctactc ctccatggtg gcgcgggaca gaatcgaact
      301 gccgacacac ggagcttcaa tccgttgctc taccaactga gctaccgcgc catattttaa
      361 aattattaat tgaataacaa gaacgacggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gttcattatg gaggttaacg
      481 ggatcgaacc gctgaccctc tgcttgtaag gcagatgctc tcccagctga gctaaacctc
      541 caacattcaa ttaaaatgac ccgtacggga ttcgaacccg tgttaccgcc gtgaaagggc
      601 ggtgtcttaa ccgcttgacc aacgggccag gtaaatttgc tacggagagt aagggattcg
      661 aacccttgag acagtttggc accatctaca tgatttccaa tcatgctcct tcggcctctc
      721 ggacaactct ccagagtgaa agaagctcaa tactctttct caaaatgag
//
LOCUS       DAZAID010000232          737 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000232 DAZAID010000000
VERSION     DAZAID010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.240"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..448)
                     /locus_tag="ACG9BE_002995"
     CDS             complement(<1..448)
                     /locus_tag="ACG9BE_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFN3033653.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT          226 a          149 c          108 g          254 t
ORIGIN      
        1 cagacacttg cttaaagcga aaaatttctt caatgtccat gcctaattct ttggatattc
       61 tcttgtctga ccatccattt tttactaatc tttggatgac aatttccatt cctcttattt
      121 catgggtccc tcttgcacga ttgtgtcgaa ttgttgaagc catacgttct tctatgggtt
      181 tatcaataac tgttaccggt acaactttta agttgagtct ttccttagca acacgatacc
      241 tatgaagcac atcaaccaag atatacttct cttctttttc cttgtaataa caaactaatg
      301 gttgagtaaa gccatcttca atgatagaaa gctccaataa gttcatttcc ggagacgcta
      361 ctcgattggg attataatcg ttcgcctgta ttttggtaat tggaacaagc cgaacgtcca
      421 tcacagggaa agttatgtct ttattcatac gcttgtatac cctactttct taaaaagtag
      481 ggtatactca agaatccgat taattatctt ctcaatttgg ataatatatc tcatcagtct
      541 tcttgtcact ttatcaacat gattttgctg caaaatggga taccgacagt actggactgg
      601 taatcaggat aaaagcaaaa ataattaatt tgtatcctaa aaaatccata aaaattctct
      661 cacattttcg ggtattaatt cgagaatcac acctataaat ttattactat ataagggata
      721 cttaaaccaa atacccc
//
LOCUS       DAZAID010000233          729 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000233 DAZAID010000000
VERSION     DAZAID010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.241"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>729)
                     /locus_tag="ACG9BE_002996"
     CDS             complement(<1..>729)
                     /locus_tag="ACG9BE_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN3033654.1"
                     /translation="TLTYSIDTHLHLSAAPYFRLLINESANLYAEFAHKSIELDNPSL
                     LFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFSIDQEITSIITFLKNEQ
                     FKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCLQKIQLSSLPRHYQQEL
                     ALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLLEQLAQIFFISPASLKK
                     HFRKVNGKSVISYFKELKMEKAKRL"
BASE COUNT          249 a           84 c          131 g          265 t
ORIGIN      
        1 gagacgtttt gctttttcca tttttagttc tttaaaatag ctaataacac tttttccatt
       61 cacttttcta aaatgttttt ttaagctggc tggactgata aaaaaaattt gggctaattg
      121 ttcaagcaaa aaattttctg aaagatgttc ttctaaatat cgtatgattg gatcaactaa
      181 ttcatctgaa taaagaatat gctttttttt aggaatgact ttctcatttg aaggtattgt
      241 caataaagca agttcctgtt gataatgcct tggtaaggaa gacaattgaa ttttttgcag
      301 acaataagta actaataagg aaagcaaatg aatgctcatc tgttggcata gtatgaaaaa
      361 agaattgatt tcctgatcat aggaaatagc tggcttacta tgttttagat aatcaatttt
      421 gaactgttca tttttcagaa aagtaatgat agatgttatt tcttgatcta tagaaaaaga
      481 ttgtcccgta atatgtgcta ataattgctc atcaaaataa aactggataa tgataaaagt
      541 ttgtgtttcc ttttgagtag aaaagatttg gacttcttgc tgtttatcaa tgaataaaag
      601 ggaaggatta tctagttcta tagatttatg tgcaaattca gcatataaat tagcgctttc
      661 atttattagt agtcgaaaat agggagcagc agataaatga agatgggtat ctatggaata
      721 ggttagtgt
//
LOCUS       DAZAID010000234          703 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000234 DAZAID010000000
VERSION     DAZAID010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.242"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..659
                     /locus_tag="ACG9BE_002997"
     CDS             <1..659
                     /locus_tag="ACG9BE_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN3033655.1"
                     /translation="NKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYK
                     ERDIYINECIEIVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQ
                     KKITVNNYILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYG
                     NNKISNMDKIFDVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS
                     "
BASE COUNT          315 a           47 c           98 g          243 t
ORIGIN      
        1 aaaataaaga aattttaaaa ttagaaaagt taaaatatga cttttttaaa ggtgcatccc
       61 atgaacttaa aacccctctt gctagtctta aaataatact ggaaaacatg aaatacaata
      121 ttggaaaata taaagaaaga gatatttata ttaatgaatg tattgaaatc gttgatagtt
      181 taacaaaaaa tatttctcaa atattatcag ttcattctat agaaaatttg aataatgatg
      241 aagaatattt gaaaataaat gatacattag aagacatatt aaaaaagtat gaaatattag
      301 caaatcaaaa gaaaataact gtcaataatt atatattaga cgagaatgtt tatataggaa
      361 aaacagcttt aaagattatt ctatctaatt tgattagtaa tgcggtaaaa tatactgatg
      421 taaatggggt aattaatatt gggatagtta atgattggtt atatatagag aattcatacg
      481 gtaacaataa aatttcgaat atggataaaa tatttgatgt taaatttgat ttaaataagg
      541 aaaatagtaa tggattaggt ttatatattg taagtaatat tctgaataat tacaatatag
      601 aatataaagt attgcaggat gaagaatttt ttatctttaa aataaaaata ttttcttaat
      661 taagatataa ttttgcaata gttattgaaa aatagagact tat
//
LOCUS       DAZAID010000235          697 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000235 DAZAID010000000
VERSION     DAZAID010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.243"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..629)
                     /locus_tag="ACG9BE_002998"
     CDS             complement(54..629)
                     /locus_tag="ACG9BE_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFN3033656.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          125 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaacg
//
LOCUS       DAZAID010000236          665 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000236 DAZAID010000000
VERSION     DAZAID010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.244"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..245
                     /locus_tag="ACG9BE_002999"
     CDS             <1..245
                     /locus_tag="ACG9BE_002999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033657.1"
                     /translation="KYRKDTLKSFRINLPNKFIGGFKMSHTLLIKDTLNILDLNINIH
                     FEENCLTKEKYKGQICLIYKGTLQYTPEEYPHLPIY"
     gene            272..538
                     /locus_tag="ACG9BE_003000"
                     /pseudo
     CDS             272..538
                     /locus_tag="ACG9BE_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(586..665)
                     /locus_tag="ACG9BE_003001"
                     /pseudo
     CDS             complement(586..665)
                     /locus_tag="ACG9BE_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
BASE COUNT          259 a          127 c          100 g          179 t
ORIGIN      
        1 aaaaatatag aaaagacacc ctgaaatcct ttagaattaa tttgcccaac aaattcatag
       61 gaggattcaa gatgtctcat actctgctta tcaaagatac cctaaatatt ctagacctaa
      121 atatcaatat ccatttcgag gaaaattgtt taactaaaga aaaatacaaa ggacaaattt
      181 gcttgattta caaagggact cttcagtaca caccagaaga atatccacat cttccaatat
      241 attaaaagaa ccttcttgaa tcattgagtg aaagaaaaga gattcgtcac tatttgagga
      301 ctgtttaact agagacatta gcgatctgcc tgaaacctat acaaccactt tacgaacgtt
      361 taaaaatttt ctgccacaga ttcaaaatgc gcttaactgc agctattcaa acagaccatt
      421 ggaatgttta aacaaccaca tcaaagtatt aaaacgcaat acgtatggct ttcgcagctt
      481 ttacaatttc aaactacgga tcatgattcg tcatggcaaa acatttttaa ccaaataaac
      541 agaagaatgt gggataaatc taaaaatcag ttttgctaca gactctcgag taaacggcgg
      601 gagcagaagc aactccttcg gaaataagcc gaaattcaca aaaatttgaa aagcaatttt
      661 cgtga
//
LOCUS       DAZAID010000237          600 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000237 DAZAID010000000
VERSION     DAZAID010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.246"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..466)
                     /locus_tag="ACG9BE_003002"
     CDS             complement(<1..466)
                     /locus_tag="ACG9BE_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033658.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYL"
BASE COUNT          194 a          106 c           61 g          239 t
ORIGIN      
        1 ccagatatgt gtgttctttt ggtttaaatg tttcaaataa atgaatcttt acaaaattac
       61 tttttgaatt ggctaaaact ttattttgat tcttatccaa tacttgatcc ccttttagcc
      121 ccataccggt atattgtaaa ataccattta accacttatc tttataaaca ttacttcctg
      181 tttgatcact aaacagtaca agagtattag tttgatgaga tcttctcatt ccaccctgtt
      241 tggaacattt aaacttctct gaaatttctt tgttagtata tactctacca acttctaact
      301 cacctaatat gtttttagac caattttctc gccatgtaat aatcttatca agattaaaca
      361 tttctccctc ttttggcatg ccatttctaa ttaaattagc taatacacca gtagaaatat
      421 ctaatttttt tagtaattct ttcttagtaa tcaatgttgg attcataaca atgcagctcc
      481 taatgatatc aatataattg cattagataa cttgaaaaat cgaaaaaaac tcattttgtg
      541 tcaagttatc tgtaatatat aaaaatatca ttattatacc tcatctatgt gaatttttac
//
LOCUS       DAZAID010000238          599 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000238 DAZAID010000000
VERSION     DAZAID010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.247"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..599
                     /locus_tag="ACG9BE_003003"
                     /pseudo
     CDS             1..599
                     /locus_tag="ACG9BE_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
BASE COUNT          218 a          121 c          124 g          136 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaaa attctaaata tttctcgtca gacctactat tatcaagcga aaccgatcga
      361 aaatgagtcc gacttagaag agatcgttca ggaagagttt attcgaaacc gaaaggctta
      421 cggtacccga aaattgaaga agtgtttagc aaagcgtggg cttcaactca gtcggcgccg
      481 aatcggtcga atcatgaaac gccgcggatt gacatctacc tatacgatcg ctcattttaa
      541 agggcaacga acagcttgta atgaagcgaa aacagcgaat gtattagatc ggaccttta
//
LOCUS       DAZAID010000239          593 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000239 DAZAID010000000
VERSION     DAZAID010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.248"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..143)
                     /locus_tag="ACG9BE_003004"
     tRNA            complement(59..143)
                     /locus_tag="ACG9BE_003004"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(107..109),aa:Leu,seq:caa)
     gene            complement(161..232)
                     /locus_tag="ACG9BE_003005"
     tRNA            complement(161..232)
                     /locus_tag="ACG9BE_003005"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Gln,seq:ttg)
     gene            complement(242..314)
                     /locus_tag="ACG9BE_003006"
     tRNA            complement(242..314)
                     /locus_tag="ACG9BE_003006"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(279..281),aa:His,seq:gtg)
     gene            complement(376..460)
                     /locus_tag="ACG9BE_003007"
     tRNA            complement(376..460)
                     /locus_tag="ACG9BE_003007"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Leu,seq:caa)
     gene            complement(481..551)
                     /locus_tag="ACG9BE_003008"
     tRNA            complement(481..551)
                     /locus_tag="ACG9BE_003008"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Cys,seq:gca)
BASE COUNT          182 a          145 c          128 g          138 t
ORIGIN      
        1 cataaaaaca aaaaaaatat agcaactctc tataagaaag ttgctatatt gataattata
       61 ccggcggccg gggtcgaacc ggcacgtcct caacggacac tggattttga gtccagcgcg
      121 tctgccaatt ccgccacgcc ggcaaattta aaaataataa ctggggtagc tggattcgaa
      181 ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc caagaataaa
      241 gggcgaatga tgggaatcga acccacgaat gccggagcca caatccggtg cgttaaccac
      301 ttcgccacat tcgccatcat aaaagatgtt atttaatttc tcaataaaaa actaagatga
      361 agacatctta gtcaataccg gcggccgggg tcgaaccggc acgccctcaa cgggcactgg
      421 attttgagtc cagcgcgtct gccaattccg ccacgccggc aagttgattt attagtacta
      481 aggcggtaac cggatttgaa ccggtgatga aggttttgca gacctctgcc ttaccacttg
      541 gctataccgc cattatacta ttatcaatct ataaaataaa gaataaatta tag
//
LOCUS       DAZAID010000240          540 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000240 DAZAID010000000
VERSION     DAZAID010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.249"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..499)
                     /locus_tag="ACG9BE_003009"
     CDS             complement(122..499)
                     /locus_tag="ACG9BE_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033659.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          155 a           98 c           99 g          188 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
//
LOCUS       DAZAID010000241          534 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000241 DAZAID010000000
VERSION     DAZAID010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.250"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACG9BE_003010"
     CDS             <1..>534
                     /locus_tag="ACG9BE_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFN3033660.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAZAID010000242          530 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000242 DAZAID010000000
VERSION     DAZAID010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.251"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..153)
                     /locus_tag="ACG9BE_003011"
     CDS             complement(<1..153)
                     /locus_tag="ACG9BE_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN3033661.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFN"
BASE COUNT          190 a           95 c           94 g          151 t
ORIGIN      
        1 attgaattca agaattgttt ctcgtatgaa tgaagcacct tcgatgtcat tggcaacaga
       61 gatttcagat aagatagaca attgattgtc gtctgttaaa aaacaaacat ctagtttttc
      121 agagctaacg tcaataccga caaataattt catggaagaa ggacctcctt tcgatataaa
      181 ttttggaaaa tgcttcgata ctgtgggatt tcccagaaac tcactgtata gccacaacct
      241 cgcctaatag aatacggtga taaaagagct agaagctgcc ttccaaacta ctatttgaaa
      301 gtctagtcca ttatcgaaac agctaatgag ttggtagtga taacagtggt tcgggtaaca
      361 gactaaacag ggtagacaaa cgctacagga ggaaatagca aattcccgtt ggatcctttc
      421 catacatcca ttgtactctg agaaatcaca caatatcgaa acaaattccg ttaggaatgt
      481 gttattcaat aaattttata atctagaaac attttgaata aaaactatta
//
LOCUS       DAZAID010000243          495 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000243 DAZAID010000000
VERSION     DAZAID010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.252"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..237)
                     /locus_tag="ACG9BE_003012"
     CDS             complement(<1..237)
                     /locus_tag="ACG9BE_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033662.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLC"
BASE COUNT          162 a           93 c           52 g          188 t
ORIGIN      
        1 acataaattt ttagcttcct tattattaga tactaattga tcaatcatag ggagccattt
       61 tttgattttc actaattgat tgctcataaa tctttcactg gacttttgtt caatcttaaa
      121 ttgcaaaact tctaactcat aattcccatc ttctgattta tttttgggaa caactatcaa
      181 acttttataa agtttatcca catcaaaatt tatctttttt gcataatttg tagccattaa
      241 tacatctcct tattcataat aaatttcata tgacgctcct ctcaaaattt accggtagac
      301 tagaaaaaag aataaaagat acgtcaattc acactttatt cttaaaaaaa aactggtctc
      361 tctaccggta atatcgattg agtccgcctt tatcgacttt atcctttttt tatattaaat
      421 tttttcaccg gtagacaggg agagaaagaa taaactaaaa agcccttaat tcttaatgtt
      481 ttcacctcct taccg
//
LOCUS       DAZAID010000244          471 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000244 DAZAID010000000
VERSION     DAZAID010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.254"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..446)
                     /locus_tag="ACG9BE_003013"
     CDS             complement(<1..446)
                     /locus_tag="ACG9BE_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN3033663.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELIT"
BASE COUNT          136 a           92 c           60 g          183 t
ORIGIN      
        1 gtaataagtt cgtatatgtt ttgcggtgtt tcttgtttct tgtaattttc cacgaatgtt
       61 tcatccatga ataaattcat aaacgcttga agtaatccta cttgttcttt tggttctttg
      121 attcctaaaa aaaatagatt ttctacagtc atttcctgat tatcccccat ttgataaaaa
      181 taaattgggt tcttcagctg cataacaaaa acaaatggct tttcgacata ttcacattcc
      241 gaatgtggca gcgcgatatt taaatactta gtaatcaatc cagtaggaaa ttctctttct
      301 cttgcaatca gtccattcaa ataattctca ttgacgtacc ccattttttc taattgttgt
      361 gcagcgatta aaaaagcttc ttctttattt tttacttcta ccgctaattg aatcaaacta
      421 ggaacgaaca tctctttata tttcattcca acctcctcct ttttgattaa g
//
LOCUS       DAZAID010000245          468 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000245 DAZAID010000000
VERSION     DAZAID010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.255"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACG9BE_003014"
     CDS             <1..>468
                     /locus_tag="ACG9BE_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFN3033664.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAZAID010000246          463 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000246 DAZAID010000000
VERSION     DAZAID010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.256"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>463
                     /locus_tag="ACG9BE_003015"
     CDS             <1..>463
                     /locus_tag="ACG9BE_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFN3033665.1"
                     /translation="GTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQ
                     PITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKY
                     TWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          189 a           68 c          108 g           98 t
ORIGIN      
        1 aaggaacaag tattgaaagt ggaacaacag tctttgcagt gaaagacggc gtaagcttac
       61 cagaagataa acttccagtg ttgaaagcaa aagatggtta tacagatgcg aaatggccag
      121 aggaagcaac tcaaccaatt acagcagatg atacagaatt tgtatcaagt gcaacaaaac
      181 tggatgataa atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat
      241 tgaataagga accagacgca tttgagggaa ttaagaacaa agaagattta ccaaaagata
      301 ctaagtatac ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg
      361 ttgtagtgac atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag
      421 acaatcgttc tgacgccgat aaatatgagc caacagtaga agg
//
LOCUS       DAZAID010000247          462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000247 DAZAID010000000
VERSION     DAZAID010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.257"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACG9BE_003016"
     CDS             complement(<1..430)
                     /locus_tag="ACG9BE_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN3033666.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAZAID010000248          451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000248 DAZAID010000000
VERSION     DAZAID010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.258"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          146 a           87 c           51 g          167 t
ORIGIN      
        1 aaaaaataaa aaaattatta cgttattaaa aaaacagatg aaagatcagg ttttattaga
       61 tctcattctt tcatcacttg aagggattta atgaactact ttttcctctt tgtcgttttt
      121 atatattcgt attccttcaa acagtaggat agcctctttc aatgcttgat atttgaactg
      181 tccgttactt aaaaatttag atgtgccgcc ctcttcctag aacatattct tttttacttt
      241 ttcattaatt gacagaccaa tcgaacgaaa ctgtaaattt cgataggata atgattggtc
      301 aaactcattc attttcaaga catctcgatt catattcaag ataggaatct ttttatcaac
      361 atcactttat tcgtattttc ctctaccata aaagcttcac gctccacaaa attgttttat
      421 acactactat tcagcccatt caacgaccaa t
//
LOCUS       DAZAID010000249          451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000249 DAZAID010000000
VERSION     DAZAID010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.259"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>451
                     /locus_tag="ACG9BE_003017"
     CDS             <1..>451
                     /locus_tag="ACG9BE_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN3033667.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DAZAID010000250          431 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000250 DAZAID010000000
VERSION     DAZAID010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.260"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            250..>431
                     /locus_tag="ACG9BE_003018"
     CDS             250..>431
                     /locus_tag="ACG9BE_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN3033668.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT          147 a           76 c           62 g          146 t
ORIGIN      
        1 aatctttttt atagagaaaa atgcaggtta atactttctt aaataaatat ttatggtgaa
       61 caaaaacaaa aaagcaagtt ttattaaacg taaccctttt catttcatag attactgtat
      121 ttattacaaa aaacgcggat ttgtcataaa ttgtcatgct tcttttacct atgaaatatt
      181 ttcacgtgtt aaagtattcc tcgttgaaca caacaaataa aactttttaa cgaataaagg
      241 agatttttca tgacatcact taaaacttta ctttttggaa caactttggc tgccggcgct
      301 gcattcttca tgggaacgac tgctcatgcg gacgaagctt ataccgttca atcaggtgat
      361 actctatcaa cgatttctca aaaatacgtt ggtgataact ccttgatcaa tgccattgca
      421 gaatcaaatt c
//
LOCUS       DAZAID010000251          415 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000251 DAZAID010000000
VERSION     DAZAID010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.261"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACG9BE_003019"
     CDS             complement(<1..339)
                     /locus_tag="ACG9BE_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3033669.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAZAID010000252          414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000252 DAZAID010000000
VERSION     DAZAID010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.262"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>414)
                     /locus_tag="ACG9BE_003020"
     CDS             complement(109..>414)
                     /locus_tag="ACG9BE_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN3033670.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          116 a           96 c           82 g          120 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggt
//
LOCUS       DAZAID010000253          400 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000253 DAZAID010000000
VERSION     DAZAID010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.263"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>400
                     /locus_tag="ACG9BE_003021"
     CDS             <1..>400
                     /locus_tag="ACG9BE_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFN3033671.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQA
                     AYAFLKRLVKQFDEPKVVVTDKAPSITSAFK"
BASE COUNT          132 a           66 c           83 g          119 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta
      241 tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac
      301 acaagcagcc tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaaggttgt
      361 agtcacagat aaagccccct ctattacaag tgcctttaag
//
LOCUS       DAZAID010000254          390 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000254 DAZAID010000000
VERSION     DAZAID010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.264"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..>390
                     /locus_tag="ACG9BE_003022"
     CDS             74..>390
                     /locus_tag="ACG9BE_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN3033672.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLE"
BASE COUNT          165 a           33 c           75 g          117 t
ORIGIN      
        1 tatatattta aattgactta ttgtgaaatc attaactata attagttcag tttatgttaa
       61 ggagaatgat gatatgaata agaaaattgt agtaagtgga ttaattatag gtggattagt
      121 gttagcaggg atcgcaggga aaaactttgc cgatgacatt ttgttatggg gtggagaaga
      181 taatattgaa caaatcaatc ataatttgga gtcattagat aaagcactag aaaataaaga
      241 gcaaaatatt agtagtttaa attcacgatt atcttcaaat aatcaagaac ttgagcaagc
      301 aaaggctaac gttgaagaat ataaacaaaa agtgttgact ttggaaaatg ataaaaatca
      361 attaactata gagaaaacaa atttagagaa
//
LOCUS       DAZAID010000255          372 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000255 DAZAID010000000
VERSION     DAZAID010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.265"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..>372
                     /locus_tag="ACG9BE_003023"
     CDS             85..>372
                     /locus_tag="ACG9BE_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFN3033673.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFL"
BASE COUNT          141 a           47 c           76 g          108 t
ORIGIN      
        1 tttgaaaacg tagtaacagg caaagaaaac tggaatgatt tatatcatgc aaacgaagta
       61 ttgaaaatag ctgaggagaa ataaatggaa attttattta ctatttgtgg tagagctggg
      121 tctaaggggt ttaaaaataa aaatttgaaa tatttggcag gaaagcccct agtccatttc
      181 gctttagcaa tgatagatat ctatcaacaa aaacatcctg aacataattg cacgacttgt
      241 ttgaatacgg acagtaatga gttagtagaa ttgacgacta agttaaatcc gtctgtgaaa
      301 attgtagaac gtaaacctga attagctaca gatactgtag ggaaaattga agtgatacgt
      361 gattcattct ta
//
LOCUS       DAZAID010000256          371 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000256 DAZAID010000000
VERSION     DAZAID010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.266"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..272)
                     /locus_tag="ACG9BE_003024"
                     /pseudo
     CDS             complement(36..272)
                     /locus_tag="ACG9BE_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          121 a           58 c           64 g          128 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaatacgg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaataac gccgtttgta taattaagcg agacaaataa
      301 aaaagccatt tatgttacac tccaattgta atacccgcta aagcatacaa ggagagtgaa
      361 cataaatgac t
//
LOCUS       DAZAID010000257          354 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000257 DAZAID010000000
VERSION     DAZAID010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.267"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          114 a           63 c           38 g          139 t
ORIGIN      
        1 tttctcagaa acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt
       61 aaaatagcga cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct
      121 ttgtgcttaa attcaaagat aattttctca ataaagattt ttgattccag taagattcct
      181 ttactccttt tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc
      241 cattaaaaat agcccaattc tctataaaga agattaagct tcattattat aatataaagt
      301 ttaaatttgg tttattactt actttttctt attctctagt aagctaatat tgaa
//
LOCUS       DAZAID010000258          345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000258 DAZAID010000000
VERSION     DAZAID010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.268"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>345
                     /locus_tag="ACG9BE_003025"
     CDS             <1..>345
                     /locus_tag="ACG9BE_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN3033674.1"
                     /translation="AEEFIRSRKAYGSRKIKKALSKRGIQISRRKISRIMKNRGLKSS
                     YTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLF
                     NREILGYSCGEH"
BASE COUNT          129 a           60 c           66 g           90 t
ORIGIN      
        1 cgctgaagaa tttatccgca gccgaaaggc ctacggctca agaaaaataa aaaaagcctt
       61 atcaaaacga ggcattcaga tcagccgacg aaaaattagt agaatcatga aaaatagagg
      121 attaaaatcg agctatactg ttgcttattt taaagtacat cattctactt gcaatgaagc
      181 caaaacgaca aacgtattga atcgtaaatt cttaagagac aacccattag aagcgatcgt
      241 aacagacttg acttatgtac gagtcgggaa aaaatggaat tatgtctgtt tcattttgga
      301 tctgttcaat cgagaaattc tcggctattc ttgtggagaa cataa
//
LOCUS       DAZAID010000259          328 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000259 DAZAID010000000
VERSION     DAZAID010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.269"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>328)
                     /locus_tag="ACG9BE_003026"
     CDS             complement(<1..>328)
                     /locus_tag="ACG9BE_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3033675.1"
                     /translation="SEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERGTNLPN
                     PQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKDIQERY
                     SDTFSLD"
BASE COUNT           89 a           56 c           58 g          125 t
ORIGIN      
        1 atctagcgaa aacgtatcac tatatctttc ttgaatgtct ttaaagactt tgtgaggtat
       61 ttctgggaag tccttaatat atagctcata ccctatatcg ataaggtatg aagttatata
      121 ttcttctaaa gttccccatt taatccagtc aattgttgaa ttagtgatag ttgataataa
      181 tttcagcttt tgtggatttg gtaaattggt cccacgttcc cagttggata tggtcgcacg
      241 tggtgaatta tctattttcc ttccaagttc ttccatagta aaaccacatg aaaggcgtaa
      301 ttgtttgatt cgttttcctg cttctgaa
//
LOCUS       DAZAID010000260          325 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000260 DAZAID010000000
VERSION     DAZAID010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.270"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>325
                     /locus_tag="ACG9BE_003027"
     CDS             <1..>325
                     /locus_tag="ACG9BE_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN3033676.1"
                     /translation="INPLIEEYANQRHSISKELFFKRANSKLQDKIRNGDFKYGEKER
                     MIDSLKQLLDEMDLAYKDISKYSSVEDFFYKITKNQFETEKFISDLSKKYNFPYEKNS
                     IIAKFL"
BASE COUNT          138 a           47 c           44 g           96 t
ORIGIN      
        1 tatcaatcct ctgattgaag agtacgctaa ccaaagacac tctatctcta aagagctatt
       61 tttcaaaaga gccaacagta aacttcaaga caagataaga aatggtgatt tcaaatacgg
      121 tgaaaaagaa agaatgattg attctttaaa acagctatta gacgaaatgg acttagccta
      181 taaagatatt tcaaagtatt catctgtcga ggatttcttt tataaaatta ctaaaaacca
      241 atttgaaaca gaaaaattta tttcagatct tagtaaaaaa tacaatttcc catatgagaa
      301 aaatagtata atcgcaaaat tttta
//
LOCUS       DAZAID010000261          305 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000261 DAZAID010000000
VERSION     DAZAID010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.271"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG9BE_003028"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG9BE_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFN3033677.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           95 a           45 c           50 g          115 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgtt
//
LOCUS       DAZAID010000262          293 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000262 DAZAID010000000
VERSION     DAZAID010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.272"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>293)
                     /locus_tag="ACG9BE_003029"
     CDS             complement(<1..>293)
                     /locus_tag="ACG9BE_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033678.1"
                     /translation="YVFEKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAF
                     SLLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYL"
BASE COUNT          111 a           49 c           36 g           97 t
ORIGIN      
        1 atcaaatata aactaactat acttgttaga acaaaaaaac aggcgttgac ttgttcttga
       61 ttagttaaaa atatcaataa aggaatactt acccaatata tttttctatt tatttcttct
      121 cctcttaaga gttgaaaaaa cggataaaaa gttagtaaac taaatgctac tggccactga
      181 tagttcaagg tggttgctct ccaaccggtt tcccccatta ctgtcaatgg tattaaacag
      241 tatatagagc aaaccaataa taaattttta acatttattt tctcaaaaac ata
//
LOCUS       DAZAID010000263          286 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000263 DAZAID010000000
VERSION     DAZAID010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.273"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..>286
                     /locus_tag="ACG9BE_003030"
     CDS             58..>286
                     /locus_tag="ACG9BE_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN3033679.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVS"
BASE COUNT           89 a           55 c           54 g           88 t
ORIGIN      
        1 agacaccctg aaatccttta gaattaattt ggctaccaat tcataggagg attcaagatg
       61 tctcatactc agcttatcaa agatacccta aatattctag acctaaatat ctatttcgag
      121 gaaaattgtt taactaaaga aaaatacaaa gggcaaattt gcttgattta caaaggaact
      181 cttctgtaca agccagaaga gtgttcccat tgtctttgtg tcgtcccctc taggatcatt
      241 cgttggggga cgacaaccgt tcggttgtta ctgaatgatg tgtctg
//
LOCUS       DAZAID010000264          282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000264 DAZAID010000000
VERSION     DAZAID010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.274"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACG9BE_003031"
     CDS             <1..>282
                     /locus_tag="ACG9BE_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFN3033680.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAZAID010000265          256 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000265 DAZAID010000000
VERSION     DAZAID010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.275"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>256)
                     /locus_tag="ACG9BE_003032"
     CDS             complement(<1..>256)
                     /locus_tag="ACG9BE_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN3033681.1"
                     /translation="KQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFD
                     NAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHL"
BASE COUNT           84 a           46 c           42 g           84 t
ORIGIN      
        1 gaaggtggtt ccaccaattg acatagtcaa ataactccaa atccaattgt tgtaaggttt
       61 caaatgtgta ttgatagaca aattctactt tcaacgactt ataagttgat tcagctacgg
      121 cattatcaaa aggacagcct ttatgactca atgatcgatt gatgtcaaaa gttgttaata
      181 attcatcaat agcttggtta tcaaactctt ttccacgatc agtatgaaaa atctcaacct
      241 ctgtcagagg ttgttt
//
LOCUS       DAZAID010000266          253 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000266 DAZAID010000000
VERSION     DAZAID010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.denovo.276"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>253
                     /locus_tag="ACG9BE_003033"
     CDS             <1..>253
                     /locus_tag="ACG9BE_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFN3033682.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DAZAID010000267        13213 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.guided.076, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000267 DAZAID010000000
VERSION     DAZAID010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.guided.076"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACG9BE_003034"
     CDS             complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACG9BE_003034"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFN3033683.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="ACG9BE_003035"
     CDS             complement(2159..3031)
                     /locus_tag="ACG9BE_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN3033684.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="ACG9BE_003036"
     CDS             complement(3373..4245)
                     /locus_tag="ACG9BE_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN3033685.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="ACG9BE_003037"
     CDS             4373..4942
                     /locus_tag="ACG9BE_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFN3033686.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="ACG9BE_003038"
     CDS             5009..5746
                     /locus_tag="ACG9BE_003038"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN3033687.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="ACG9BE_003039"
     CDS             5977..7245
                     /locus_tag="ACG9BE_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN3033688.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="ACG9BE_003040"
     CDS             7732..8196
                     /locus_tag="ACG9BE_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN3033689.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="ACG9BE_003041"
     CDS             8180..8809
                     /locus_tag="ACG9BE_003041"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN3033690.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACG9BE_003042"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACG9BE_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFN3033691.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="ACG9BE_003043"
     CDS             10688..11134
                     /locus_tag="ACG9BE_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFN3033692.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="ACG9BE_003044"
     CDS             complement(11333..11848)
                     /locus_tag="ACG9BE_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN3033693.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="ACG9BE_003045"
     CDS             11965..13167
                     /locus_tag="ACG9BE_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN3033694.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAZAID010000268        12355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.guided.082, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000268 DAZAID010000000
VERSION     DAZAID010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.guided.082"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACG9BE_003046"
     CDS             complement(134..577)
                     /locus_tag="ACG9BE_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFN3033695.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACG9BE_003047"
     CDS             complement(795..2135)
                     /locus_tag="ACG9BE_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN3033696.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACG9BE_003048"
     CDS             2404..3300
                     /locus_tag="ACG9BE_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFN3033697.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACG9BE_003049"
     CDS             complement(3450..4391)
                     /locus_tag="ACG9BE_003049"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN3033698.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACG9BE_003050"
     CDS             4517..5638
                     /locus_tag="ACG9BE_003050"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN3033699.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG9BE_003051"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG9BE_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN3033700.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACG9BE_003052"
     CDS             7465..7992
                     /locus_tag="ACG9BE_003052"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN3033701.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="ACG9BE_003053"
     CDS             8203..9264
                     /locus_tag="ACG9BE_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFN3033702.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="ACG9BE_003054"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="ACG9BE_003054"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFN3033703.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="ACG9BE_003055"
     CDS             10177..12159
                     /locus_tag="ACG9BE_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFN3033704.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..>12355)
                     /locus_tag="ACG9BE_003056"
     CDS             complement(12224..>12355)
                     /locus_tag="ACG9BE_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFN3033705.1"
                     /translation="NQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         4088 a         2260 c         2577 g         3430 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgatt
//
LOCUS       DAZAID010000269         9613 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.guided.103, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000269 DAZAID010000000
VERSION     DAZAID010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.guided.103"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..138)
                     /locus_tag="ACG9BE_003057"
     CDS             complement(<1..138)
                     /locus_tag="ACG9BE_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFN3033706.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IE"
     gene            283..468
                     /locus_tag="ACG9BE_003058"
     CDS             283..468
                     /locus_tag="ACG9BE_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFN3033707.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            626..793
                     /locus_tag="ACG9BE_003059"
     CDS             626..793
                     /locus_tag="ACG9BE_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033708.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            1411..2070
                     /gene="vanR-B"
                     /locus_tag="ACG9BE_003060"
     CDS             1411..2070
                     /gene="vanR-B"
                     /locus_tag="ACG9BE_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFN3033709.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            2073..3434
                     /gene="vanS-B"
                     /locus_tag="ACG9BE_003061"
     CDS             2073..3434
                     /gene="vanS-B"
                     /locus_tag="ACG9BE_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFN3033710.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(3525..4826)
                     /locus_tag="ACG9BE_003062"
     CDS             complement(3525..4826)
                     /locus_tag="ACG9BE_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN3033711.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            5105..5911
                     /locus_tag="ACG9BE_003063"
     CDS             5105..5911
                     /locus_tag="ACG9BE_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFN3033712.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            5929..6636
                     /locus_tag="ACG9BE_003064"
     CDS             5929..6636
                     /locus_tag="ACG9BE_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFN3033713.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            6633..7604
                     /locus_tag="ACG9BE_003065"
     CDS             6633..7604
                     /locus_tag="ACG9BE_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFN3033714.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            7597..8625
                     /gene="vanB"
                     /locus_tag="ACG9BE_003066"
     CDS             7597..8625
                     /gene="vanB"
                     /locus_tag="ACG9BE_003066"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFN3033715.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            8631..9239
                     /locus_tag="ACG9BE_003067"
     CDS             8631..9239
                     /locus_tag="ACG9BE_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFN3033716.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(9305..9478)
                     /locus_tag="ACG9BE_003068"
     CDS             complement(9305..9478)
                     /locus_tag="ACG9BE_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033717.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         2759 a         1937 c         2401 g         2516 t
ORIGIN      
        1 ttcgatgttg gcaagatagc gccagtcaat atttattttc tctgccattg ctttgcgcga
       61 aacgcgcttt gcttgccggg ccgctttcac atccgcgccg aaagtttcaa agccgggaca
      121 atcttcaata ttcgccatat cgcacctccc acttacattg tatatttcat actctgcccg
      181 tggaatgttg gtatatgcac ctttcttaaa gtttatattt cacattttgt ttcttttatt
      241 cttcgagcaa aagaattata atgtaccctg tggaggtgta ctatggacta catgacgttg
      301 aaagaggcag gcgagaaatg gggtgtgccc ccacgatgga taaactacta ttgtgctgcg
      361 ggacgcatcc ccggcgctgt aaagatggcg acgatctggc tcattcccaa aactgcggaa
      421 aagccgattg acaggcgtac aaagaaagga aaggcggtta ctgaatagta taatgaaacc
      481 ggcgcatttt ggatatggtt ttgaagagag agaaagcatg gtattttgtc acggaaaaac
      541 tataagatga tagatggcag gctgctccag accgataaaa agtattctca tctgaaacag
      601 aaagaaaaga tttacacatg gatgtatgaa gaagcgaaac tttatcatga cagaaatgga
      661 aaatgcccga aacaaagacg aggaagctgc tgctgtatca gccgtatatg gtagaattga
      721 aaaggccggc atctggattc tttatggtga agtctccaga cattggataa ctgctggtct
      781 aatgcgcctt tgagtatggg gtgattgatt ttaataccgg ggaggtacgg ctttttgagg
      841 aaaatattct gggatttaca tttggcgatt attacatttc ctgtaactgc aaggacacgg
      901 atgatgaaga tatgaggaat atagacttgc tgattttcta ctgtcttgaa cggtattagg
      961 actgaatctg ctgcgcggag tgcggcaaga gcgaccggag ggagtacaca caggccctgc
     1021 ctgtgtgaaa agccataccg caaggtgcat tcgcaaaaaa gaggccgcgc attttgctga
     1081 ggcttggggt gtccccaaca agcaaaacga gaacgccgca ggcgggcaag tggtggatca
     1141 gaatatccgt aaaggatatt ctgatccatt gcttgccagt attatcggga tgttttgaca
     1201 accgctgtaa tcgaaacatc ccaaacatta agaaggtgtt aattattatt atcttgattt
     1261 aattcgttta aaccaataat atagatacat acaccatgaa aagaagcgag tatcccaatg
     1321 acggatgatt aaaatccgaa aagaaatcgc tttgaaacta cagggaaact acagactgtt
     1381 atgttatctt cttaaaatgg agggattttt atgtcgatac gaattctact tgtcgaggat
     1441 gatgatcata tctgtaatac agtaagggcg tttctggctg aggcaggata tcaggtggat
     1501 gcctgcacag atggaaatga ggcatacacc aagttttacg aaaacactta tcaactggtt
     1561 attcttgata ttatgctgcc cggtatgaac gggcatgaac ttttgcgtga atttcgtgcg
     1621 aaaaatgata ctcccattct gatgatgaca gccctgtcgg atgacgaaaa ccaaatccgg
     1681 gcgtttgatg cagaggcaga cgactatgta acaaagccat tcaagatgca gattttacta
     1741 aagcgggtgg aagccctgct gcggcgcagc ggtgcgctgg caaaggaaat ccgtgtcggc
     1801 aggctgacac ttctgccgga ggatttcacg gtactttgtg acggtacaga gctgcccctg
     1861 acacgaaaag aatttgaaat ccttttgctg ctggtgcaga acaaaggcag aaccttaacg
     1921 catgaaatca ttttgtcacg tatatgggga tatgactttg agggtgatgg cagcacagtc
     1981 cacactcata tcaaaaattt gcgggccaag ctgccggaaa atatcatcaa aaccattcgc
     2041 ggtgtaggat accgattgga ggaatcataa taatggaaag aaaagggatt ttcattaagg
     2101 ttttttccta tacgatcatt gtcctgctac tgcttgtcgg tgtaacagca acactgtttg
     2161 cacagcaatt tgtgtcttat ttcagagtga tggaattaca gcaaacagta aaatcctatc
     2221 agccattggt ggaactgatt cagaatagcg ataggcttga tattcaagag gtggcagggc
     2281 tgtttcacta caataaccaa tcctttgagt tttatattga agataaagag ggaagcgtac
     2341 tctatgccac accaaatgcc aatacatcaa atagttttag acccaacttt ctttatgtgg
     2401 tacatagaga tgataatatt tcgattgttg ctcaaagcaa ggcaggtgtg ggattgcttt
     2461 atcaagggct gacaattcgg ggaattgtta tgattgcgat aatggttgta ttcagccttt
     2521 tatgcgcgta tatctttgcg cggcaaatga caacgccgat caaagcctta gcggacagtg
     2581 cgaataaaat ggcaaacctg aaagatgtac cgccgccgct ggagcgaaag gatgagcttg
     2641 gcgcactggc tcatgatatg cattccatgt atgtcaggct gaaagaaacc atcgcaaggc
     2701 tggaggatga aatcgcaagg gaacatgagt tggaggaaac acagcgatat ttctttgcgg
     2761 cagcttctca tgagctaaaa acgcccatcg cggctacaag cgttctgttg gagggaatgc
     2821 ttgaaaatat cggtgactac aaagatcatt ctaagtatct gcgcgaatgc atcaaaatga
     2881 tggataggca gggcaaaatc atttccgaaa tactggagct tgtcagcctg aatgatggga
     2941 gaatcgtacc catagctgaa ccgttggaca tagggcgcac ggttgccgag ttgctgcccg
     3001 attttcaaac cttggcagag gcaaacaacc agcggttcgt cacagatatt ccagccgggc
     3061 aaattgtcct gtccgatccg aggctgctcc aaaaggcact atccaatgtc atattgaatg
     3121 cagttcagaa cacgccgcag ggaggcgagg tacggatatg gagtgagcct ggtgctgaaa
     3181 aatgccgcct ttttgttttg aacatgggcg ttcacattga tgatactgcg cttccaaggc
     3241 tgttcacccc attctatcgc attgatcagg cgcgaagcag aaaaagtggg cgaagcagaa
     3301 aaagtgggcg aagcggttta ggacttgcca tcgtacaaaa aacgctggat gccatgagcc
     3361 tccagtatgc gctggaaaac acctcggatg gcgttttgtt ctggctggat ttaccgctca
     3421 catcaacatt gtaaatattt aaaacttaaa tgattttggg ctctttgtca aataggactg
     3481 atagagtgag ataagaacat ctaaggtaga attccttttc tactttagat gttttttgat
     3541 tcataggtaa tcagattatt tagtaagaaa attctggtct tcattttccg aaagcttcga
     3601 aagccataag cgactcgttt caatgctttg atgtgggtat gcagattttc tattttggca
     3661 tttgaataag gcaattcgag ggctaaacga atgccggatt catagtttat aaaagctttt
     3721 tttatagaat gcagttcttt tggaacggaa ttgggcatcg ttcttacaag tttaaagaaa
     3781 gtgtccgcct ctttgtcgtg aaacgcttga agaatttctt gatagtaaaa ataggcgatt
     3841 cgtaattcgt cagagatcga aagcagacga tccaccactt cactttcggt gagatacgcc
     3901 caaccaaaat tgcgccattt ggtccatttg tcataaacaa gcttgtcctg tcgtttgatc
     3961 agaagccgag acaacgcctt taattgtcga taatatttgg cttgtgttgg atcgtttcga
     4021 tcaaagcttt tcatgattcg tacgcgaata tcctcaaatt tacgaatgag atgtttgaca
     4081 atgtgaaaac gatccacaac gagttttgcc tttggaaaac attcctttat cagagaggca
     4141 taagaaacat tcatgtccgt cacgatgacc tttaccttca atcgtgcgcg tcgttcaaat
     4201 tgcataaaat aagaaacgag agcctttttc ttccgtgaag gaagaatatc cagtaatttt
     4261 ccagtatcgc catccataca agaaaaggag tagggaccga cttgattttt cagtgaacga
     4321 aattcatcca ttaataaaca acgcggtaac caatttcttt ttacttttgt catggggatc
     4381 agagaagcga gtgtccgaga aacggtcgac caagaaacgc cgcaatgctt agcaattgtc
     4441 gtcattgctt gtttttcagt tagctcttct aaaattttat agcgcacttg tttagcgata
     4501 aagcagtttc tatcgacaat gtaggtattc gccgtaaaat aggtgtggca gccacgacag
     4561 tggtagcgtt gtttcgctaa agcaagataa gtcagttggt tcgcacattt attaagttga
     4621 atgagcgatt ttttctttcc gttcttaacg atcatgcctt catgattcat ctgtttacac
     4681 aaaggacagc gacgaatttc tttacttaac gtcgcagaaa caacaagact cttgatattc
     4741 ttaatgctct tcgattccac atttgtgatg aaaatgtttt tatctttgat tccaagtagt
     4801 agtttgatat gatgaagatc aggcatacag aacgctcctt tgattgtgtt gtggtgattc
     4861 aattctaacg gagcttctgt atgttttgtt ttttcatgct caaaagggac cgatagaatg
     4921 attttctatc agtcctagaa attatagagc cgattttgac cgacaggtat aaccctgccg
     4981 gtcttttttt gctgctacag gaaaactaca gactggctac agggaaagta cagatgcgct
     5041 tgccataata acaatcgtac cagccacaaa tcgtagtttt attgcaaagg aggcattcga
     5101 tcaaatggaa aaaagcaact atcattccaa tgcggatcat cacaaacggc atatgaaaca
     5161 atccgtggaa aagcgggctt ttctatgtgc gttcattatt tcgttcacag tctgcacgct
     5221 gtttttgggg tggagactgg cttccgtatt ggaggcaaca cagataccgc ccatccctgc
     5281 aactcataca ggcagcagca ctgacgtagt ggagaatttg gaggaaaacg ctcttgccac
     5341 cgccaaagaa cagggagatg aacaggaatg gagcctgatt ttagtgaaca ggcagaaccc
     5401 catccccgca cagtacgatg tggagcttga gcaactatca aatggtgagc ggatagatat
     5461 tcggatttct ccctatcttc aagatttgtt tgatgccgca agaactgatg gagtttaccc
     5521 gattgtcgca tccggatacc gaacaacaga aaaacagcaa gaaattatgg atgaaaaaat
     5581 tgccgaatat aaggcgaaag gctacacctc tgcacaggct aaagcggaag cagaaacttg
     5641 ggtggccgtg ccgggaacga gcgagcatca gcttggtctt gctgtggata tcaatgcgga
     5701 cggaattcat tcaacaggca acgaggttta tagatggctg gatgaaaaca gctatcgttt
     5761 tggttttatt cgtcgctacc caccagacaa gacagagata accggtgtga gcaacgagcc
     5821 gtggcattac cgatatgttg gcatcgaagc tgccacagag atgtacaacc aaggggtttg
     5881 ccttgaggaa tatttaaaac cagaaaaatg agaaaaggat gttatgttat ggacagaaaa
     5941 agattgacac agcgtttccc gttcctgctt ccaatgagac gggcgcagag aaaaatgtgc
     6001 ttttatgcgg gaatgagatt tgacggctgt cgctatgcgc agacgatagg ggaaaaatcg
     6061 ctttcccatt tgctctttga aacggattgc gcattataca accacaatac cggatttgac
     6121 atgatatacc aggaaaacaa ggtgttcaac ttaaagctgg cagcaaagac cttaaacggc
     6181 ttattgataa gaccggggga aaccttttct ttctggttgt tcctgcatac gccattgaca
     6241 attatccagc gcagaggtca cgaagtaaag gagtttccag agccaaacag tgacgagatt
     6301 aaaggggtgg atgcaaccat ttcagagggc tggattgatt taaaagtgcg aaacgatacc
     6361 gactgcacct accaaatatg ggtgacctta gatgatgaga aaatcatcgg tcaggtgttc
     6421 gccgacaaag agccccaagc attatacaaa attacaaatg gcagtatcca gtatgtccgt
     6481 gaaagtggcg ggatttatga atatgcccag gttaaacgga tgcaagttgc cttaggtacc
     6541 ggggaaataa tagattgcaa gctgctttat acaaacaaat gcaaaatctg ttatcccctc
     6601 ccggaaagtg tggatattca ggaggagaac caatgagaaa aagtgtgggc attactgttt
     6661 ttggatgcga acaggatgag gcaaatgctt tccgcgcttt atcgccggat tttcatatta
     6721 tccctacgct gattagcgat gcgatatcgg cagacaacgc aaaattggcc gctggcaatc
     6781 aatgcgttag cgtaagccat aagtccgagg tttccgaggc gacaattctt gcgctgagaa
     6841 aggtcggggt aaaatacatt tctacccgca gcatcggctg cgatcacatt gatacgactg
     6901 ccgccgagag aatgggaatc tcggttggca cggttgcgta ttcgccggac agcgttgcgg
     6961 attatgcttt gatgctgatg ctgatggcca tacggggtgc aaaacccacc atgcacgccg
     7021 tggcgcaaca agatttcaga ttggatcgta tccgggggaa agaactgggg gatatgactg
     7081 tgggagttat tggaaccggc catatcgggc aagcggtcgt caaaaggctg cggggatttg
     7141 gatgccatgt gctggcctat gataacagcc gaaaaatgga tgcagattat gtccagcttg
     7201 atgagcttct aaaaaacagc gatattgtta cgctccatgt gccgctttgt gcggataccc
     7261 gccatctgat cggtcagaag caaattggag agatgaagca aggcgcattt ttaatcaaca
     7321 ccgggcgcgg agcgcttgtc gataccgggg cgctggtgga ggcgttggaa agcggaaagc
     7381 tgggtggtgc ggcactggat gtgttggagg gcgaggatca gtttgtttat accgattgct
     7441 cgcagaaagt gcttgaccat ccctttttgt cgcagctttt aaggatgccg aatgtgatca
     7501 tcacacccca tacggcgtac tacactgagc gtgtgctgca ggatactaca gaaaaaacaa
     7561 tcaggaattg tctcaatttt gaaaggagtt tacagcatga atagaataaa agtcgcaatc
     7621 atcttcggcg gttgctcgga ggaacatgat gtgtcggtaa aagccgcaat agaaattgct
     7681 gcgaacattg atacggaaaa attcgatccg cactacatcg gaattacaaa aaacggtgta
     7741 tggaagctat gcaagaagcc atgtacggaa tgggaagccg acagtctccc cgccatactc
     7801 tccccggata ggaaaacgca tgggctgctt gtcatgaaag aaagcgaata cgaaacacgg
     7861 cgtattgatg tggctttccc ggttttgcat ggcaaatgcg gggaggatgg tgcgatacag
     7921 gggctgtttg tattgtctgg tatcccctat gtgggctgtg atattcaaag ctccgcagct
     7981 tgcatggaca aatcactggc ctacattctt acaaaaaatg cgggcatcgc cgttcccgaa
     8041 tttcaaatga ttgataaagg tgacaagccg gaggcgggtg cgcttaccta ccctgtcttt
     8101 gtgaagccgg cacggtcagg ttcgtccttt ggcgtaacca aagtaaacgg tacggaagaa
     8161 cttaacgctg cgatagaagc ggcaggacaa tatgatggaa aaatcttaat tgagcaagcg
     8221 atttcgggct gtgaggtcgg gtgtgcggtc atggggaacg aggatgattt gattgtcggc
     8281 gaagtggatc aaatccggct gagccacggt atcttccgca tccatcagga aaacgagccg
     8341 gaaaaaggct cagaaaatgc gatgattaca gttcccgcag acattccggt cgaggaacga
     8401 aatcgggtgc aggaaacggc aaagaaagta tatcgggtgc ttggatgcag agggcttgcc
     8461 cgtgttgatc tttttttgca ggaggatggc ggcatcgttc taaatgaggt caataccctg
     8521 cccggtttta catcgtacag ccgctaccca cgtatgatgg ccgccgcagg aatcacgctt
     8581 cctgcactga ttgacagcct gattacattg gcgttaaaga ggtgataagt atggaaaatg
     8641 gttttttgtt tttagatgaa atgttgcatg gcgtccgttg ggatgccaag tacgccacat
     8701 gggataactt cacaggaaaa ccggtggatg ggtatgaggt gaatcgcatc atcggcacaa
     8761 aggccgtggc gtttgctctg cgcgaggcac aaatccatgc ggctgcgctt ggctatggct
     8821 tgcttttatg ggatggatat cggccaagaa ctgcggtgga ctgcttcctg cgttgggcag
     8881 cgcaaccgga ggacaatctc acaaaagaaa aattttaccc caatatagag cgagccgagt
     8941 tgattacaaa gggttatgtg gcctcacaat ccagccatag ccgtggaagc gcaattgatc
     9001 ttacgctcta ccacctggat acaggggaac ttgtttcaat gggaagtaac ttcgatttta
     9061 tggacgaacg gtcgcatcat acagcaaaag ggatagggga tgcagaggca caaaatcgaa
     9121 gatgcttgcg taaaatcatg gaaagcagcg gatttcagtc ttatcgcttt gaatggtggc
     9181 actataagtt gattgatgag ccataccccg atacctattt taattttgcc gtttcataat
     9241 gaaagtattg gttttgtatc agattttgga tttgataaaa tctatgctgg tcatggacaa
     9301 tcttttactg gaaagtggtt aaagcatctt ttgtaataca ttgcaaatta tagtattcaa
     9361 tctgcccagc ggcagaaaag aaaaaaaccg ctgttgggca gacctttccc acgcccattt
     9421 tgtattttgc tggcggcggt tttgagaaat caaggccgcc gttcttttct ggcagcaaaa
     9481 ggtgtggcgg cccataggag gatgccgcta tgctgttttg ctattgccat gctctaatcc
     9541 acttccacca ctcaaaaaaa gccttgcccc tccatgaact gaaccccaaa agttgaacta
     9601 tttaatggac tgt
//
LOCUS       DAZAID010000270         4574 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.guided.153, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000270 DAZAID010000000
VERSION     DAZAID010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.guided.153"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..750
                     /locus_tag="ACG9BE_003069"
     CDS             58..750
                     /locus_tag="ACG9BE_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN3033718.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1077..1574
                     /gene="dfrG"
                     /locus_tag="ACG9BE_003070"
     CDS             1077..1574
                     /gene="dfrG"
                     /locus_tag="ACG9BE_003070"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFN3033719.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1646..3598)
                     /locus_tag="ACG9BE_003071"
     CDS             complement(1646..3598)
                     /locus_tag="ACG9BE_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFN3033720.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3605..3841)
                     /locus_tag="ACG9BE_003072"
     CDS             complement(3605..3841)
                     /locus_tag="ACG9BE_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033721.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4243..4533
                     /locus_tag="ACG9BE_003073"
     CDS             4243..4533
                     /locus_tag="ACG9BE_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN3033722.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         1542 a          764 c          789 g         1479 t
ORIGIN      
        1 gtgtttgaga tcacttactt ttagcatgta aaagtattgg attaaggagc tatgcaaatg
       61 aaaaagaaat ttattactgg tgggattgtc gttgcatttc tatgtctaat tggctacaca
      121 ggcacgcggt acgcaaatga aattattttg tggggtggag agacagatat tgatacaatc
      181 aatgagaatc tgacacagct tgatcataca ttgacagata aagagcaagc gattacagat
      241 cttaccgctc gactagcaca aaaagaacag acattgcaag attcaaaggc agatgtccaa
      301 gaataccaaa caaaagtaaa tgaattagaa aaccaaaaaa atcaatcggc agcagaaaaa
      361 caaaacttag aaagtcaatt agtcagcaag gatgcacaac tacaaagcaa acaaaatgaa
      421 atcaatgtca aactagatga gatcaaccaa aaaaatgatg aaatcaatca tctgaatgca
      481 gattggacag caagactaca agaagcacaa gcaaaacttt cagaattaca aaatactgta
      541 aatggtttga atcaacaaat cgcaacactg acacaagaaa aaacaaatgt agaaactcag
      601 ttaacagata cacaaaagaa attagacgaa gcaactgctg aaaactcaag ctggaaacaa
      661 tatattcaaa agttagaaaa agcaaaagat gaagtggaag atacagctga taaatctcag
      721 caaattgttg aagaacattt gcgtaaataa tctttcttaa caagtaaacc taaattagta
      781 aagaagcaac agtaatctat actaagtagt tcagttttgg agtacaaaat gacaaaatgt
      841 acttcaaaat ggactacttt tttcatttta acttacatac caaattacat acaaaatgat
      901 ttataaaatg caattgaaaa tgataattga atgaaaattt gtgattttgt ggtattatga
      961 taatataact tatagaaaaa attgcttatt caactaacgg gcaggataat gagaaaaggt
     1021 tttttgatag aaataagaaa ttagatggta aattagttaa aaaggctgtg ttataaatga
     1081 aagtttcttt gattgctgcg atggataaga atagagtgat tggcaaagag aatgacattc
     1141 cttggaggat tcccaaggac tgggaatatg ttaaaaatac tacaaaggga catccgataa
     1201 tattaggtag gaagaacctt gaatcaatcg gaagagcctt acctgacaga agaaatatta
     1261 ttctgacgag agataagggg tttaccttta atggttgtga aattgttcat tcaatagaag
     1321 atgtttttga gttatgtaaa aacgaagaag aaatttttat tttcggagga gaacagattt
     1381 ataatttgtt tttcccttat gttgagaaaa tgtacatcac aaaaatacat catgaattcg
     1441 aaggagatac tttttttcca gaagtgaatt atgaggaatg gaatgaggta tttgcccaaa
     1501 aagggataaa gaatgataaa aatccgtata actactattt tcatgtatat gaaagaaaaa
     1561 acttattgag ttaacgggtg cgttaattga agaagaacag taaagataaa gcacaagagc
     1621 ttgggattac tccaagctct ttttgtcatt tgaaataaat tatatctttc caatcaaatt
     1681 gtttatctgt aatccctaat ctttgagcag gtgtcaaaac ctttttatcc gctgatttaa
     1741 aagggcgaca aaagttgtag taagttctta gaattgtaat cgcaaattga gcatacttcg
     1801 gattgaagtt agcgtaaata tagctcttct tatcaccacg agctgttgtt aatggtcttt
     1861 caagagatga tatataacgt cttatctgtt gaataaagct attggtcgaa tggtcattaa
     1921 catttaatag catctttgcg atttcgtttg gttccagagc agataaatcc gttctacaat
     1981 caactgaata aaatccttta tccttagtgg ctaatggatg tttgataggg ttatcggcat
     2041 atttgcgata tctttctcca tctttactaa tagcctcttc gtggaattga tgacttgtta
     2101 gtaattcagt tagataatga gatgccaaag tccgtagaaa ctttgttttg aatcctctga
     2161 tatctcccca atcgagcagt tctgcttttg cttgtccgaa ttcttttaag cattgttttc
     2221 tgcttttcgt cttgttaact tggcaaataa aatgatgtgc atcgctcaac cttaattctt
     2281 tcgtgaatac tctataaaag gcgtttctaa tagagaagtc atcatcacta atcattcgcc
     2341 attcgtcggc attaaccatt tgtttaatta accaatagtg agcagttgta gtataaggag
     2401 catcaacatt taaaccatcg acaaatctac tcctattagt aattttccct aattcgtgaa
     2461 gataagcatt cctattttcg ctatcgttag cggatggttc ttgtggataa taagaccaat
     2521 ctaacctatc atttttacga ctaaaagtat tgagatggtc ttctttgaat tttcttgtat
     2581 cttcatttaa ttcatccatt gaaatattcc aatcataggc aacatcagaa cggaacacat
     2641 atcttgataa gacctcagca gatacaacta tatacgtttg catattaagg tcttcaaacc
     2701 ccgtatattt ctttgaacct tgtcctttct tacggacgtt atttaggtag tagtgcatct
     2761 tgtccgtatt aagccacatc tcgttaaatt tctttgtttg aaggggttgt gtctcatatc
     2821 gttctaaaaa ctccaaacaa cgcctataca gccactccag cttatgatag taggttccaa
     2881 ctccaattcc taaaatatca caagtacgac tgactgaaac ccttccaaca accatcttag
     2941 cgaacattgg aagaatggtg ttcttttgtt ggtgataagt agtggtttct tctctttttg
     3001 gtagaacatt agtgaacttc ttacacgcct tacattgata gcgttgagat ttgcccctac
     3061 ttttccccct tttgtaaaat tgcttcggct cattgaatgg ggttgattct tcttccgagc
     3121 agccttcttt atgaaattga taatctggct caacatcttg gatgctattg atttctataa
     3181 ggcgtttaat ttcttcaaca acagaccaat tagataaagg ggttactgta cagttttgcg
     3241 atacacctct gccaatagga ttgtcattgc agtacatacc tttatgacct ttatctttgg
     3301 aactaccagt cattttatat cgacttggtt tcccttttac atctttgaat ttgtgctgtt
     3361 ctttcccaaa attacaacaa aacggattga tgcagtaatt atattgaatg ggatattcag
     3421 aaccgttcca tttaaaagac gttggcaaga acaacatttt gttatacctt gttgcaaatc
     3481 gttttggagt taataaatca tattgtttag ccctatcttt taattcttcg tttgaaatcg
     3541 gaattttaac atctacaatt tcatcttcct tcgtggctaa tcttttaagc ttggtcattg
     3601 tctatcaacc cactctatct ttgataccca ttttttcaac aatccgctta ttacttcgct
     3661 tgttggcaat ccattcaata aacttcttat cgtctaaaat gttcaataag aattcatcca
     3721 ctgcttcgct ttcagtacgc tctgcatatt cagcgtattg cttaactatc acccttactt
     3781 gctctgatat ttcccaatcc acagacttag cgttcttatt ttttggctca agaaattgca
     3841 taggaaaatg cacccctttt tgcttagtaa attgtaacca ttttaacaca ccaatttgca
     3901 ttcctacaat aattatttca cgttcgcatt tttatataca ttttaactta cattttgtgg
     3961 tgtaaattaa caacaaacgt tgatttaaca ataaaaaacg agtatcaaat tcagttgcaa
     4021 tttgatactc atttaggttt acattttcac tttttgtact ccaaaactga actacttaaa
     4081 tctatactgc tgcctctttt ttttagtaca tgtccttcat cttcgcagga agtgccaata
     4141 tgattttaag aaaggtaata gacaaaaagt caaaaagata aaacagagtt tcttctttac
     4201 aaagggaatg tatgttcgta taatggtatt gaggtgaagg ttatggagaa aaaatatgga
     4261 acagtagata agatccgtac tttgcaagtt agtgccagac cgttgatccg tttcagttta
     4321 aatggggtca attgtttgat tgcttcccat agtttgaact tcctagctga agtagacgaa
     4381 gggatgaagt tagtcgttac aggttattat aataatagaa agcaatttgt tgtgagatcc
     4441 tatcatgtgt tagggaaacc caaaattgtg gtagaatatg agaaaagtct atacccaaga
     4501 aaaaaggtcc gacaagatga aagaataaac tagaaaacaa gcggctgaga caaaaatgtc
     4561 ctagccgctt gttt
//
LOCUS       DAZAID010000271         3795 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.guided.170, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000271 DAZAID010000000
VERSION     DAZAID010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.guided.170"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..193
                     /locus_tag="ACG9BE_003074"
     CDS             110..193
                     /locus_tag="ACG9BE_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFN3033723.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="ACG9BE_003075"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="ACG9BE_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFN3033724.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="ACG9BE_003076"
     CDS             complement(1318..2181)
                     /locus_tag="ACG9BE_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN3033725.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="ACG9BE_003077"
     CDS             complement(2183..2455)
                     /locus_tag="ACG9BE_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN3033726.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="ACG9BE_003078"
     CDS             complement(2472..2681)
                     /locus_tag="ACG9BE_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFN3033727.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2759..3655)
                     /locus_tag="ACG9BE_003079"
     CDS             complement(2759..3655)
                     /locus_tag="ACG9BE_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN3033728.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1174 a          662 c          586 g         1373 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattct
     2761 attctttttc attttctaat tgaatgattc gctcaatcat ttcaaaaaat acgttctcat
     2821 acatagacaa aacttttttg tcatagcctt tgtgttctgt aatgccattt ttagaccaag
     2881 tacttacttt attacttcgc ttgataatat ttcggaaaac caaattatcc tctttatgtt
     2941 ctttgtacag ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag
     3001 gaacaaaacc aatcatatct agtccagggt taaactgttc ttgcaaatca atcaaatagg
     3061 aaatatagtt ttgaatgttg tttgtacttt cttcttctgc ttgtaaaggg atcataacgt
     3121 aatcactcgc cacgattgca ttatttgtat aaacgcttgg cgttggtaca gtatcaataa
     3181 taataagatc atagtcactt tttaaaggtg ctaaaagagt agcaagcaat ctactttcat
     3241 tttcaaacgt ccatgagcga gttaattttg gcagtaacat caaatcaaac gtgccaggga
     3301 tcaagtctaa attatcagtc aaatgaataa tagaagaagc caagtttcca tttttcaagc
     3361 cttcataaaa attgacacgt ggcaattcta cctcaaaagt ttttgctaag tcttttgtca
     3421 atgttgcctg taagtcctta tcgatcatta aaacttttaa attcaatttg tctgtcaagt
     3481 aagcaaacat agtcgataat ttggactttc caacaccacc tttaaagtaa ttattcaaga
     3541 taataattgc ttcattttta ttgtttaaaa tacgtcttaa ttcttcaagt atttttagtt
     3601 tttccttctc cataactacg ccccattctt tttttgtata gtaatattgt atcattaaga
     3661 atcacaaatc acaagtgatt tgtgattaat cacttgtgat ttgtgataag tgatttgtga
     3721 ttaatatata aaagccctct ttaaagggct tttataggtt ctgttgcaaa gttttaaata
     3781 aagaataaaa tccct
//
LOCUS       DAZAID010000272         3020 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.guided.182, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000272 DAZAID010000000
VERSION     DAZAID010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.guided.182"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..500
                     /locus_tag="ACG9BE_003080"
     CDS             <1..500
                     /locus_tag="ACG9BE_003080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN3033729.1"
                     /translation="YHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREI
                     FKTAVFNSNQSVLLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEI
                     ENSHEIAEVIMVLSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAF
                     IQFCK"
     gene            607..1845
                     /gene="mef(H)"
                     /locus_tag="ACG9BE_003081"
                     /gene_synonym="mefH"
     CDS             607..1845
                     /gene="mef(H)"
                     /locus_tag="ACG9BE_003081"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFN3033730.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            2072..2491
                     /locus_tag="ACG9BE_003082"
     CDS             2072..2491
                     /locus_tag="ACG9BE_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN3033731.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            2496..2735
                     /locus_tag="ACG9BE_003083"
     CDS             2496..2735
                     /locus_tag="ACG9BE_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN3033732.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT          989 a          410 c          546 g         1075 t
ORIGIN      
        1 tttatcatca tttcgattca aaggaagata ttttaaaagc aatttttcaa agaattggaa
       61 atgaaaatgc agtaatattt gctaaaatta gagataacga tagtttaaac ggtttagaaa
      121 agttaagaga aatatttaag acagcggtat tcaattctaa ccaaagcgtt ttattgacag
      181 tttctcctca actattaaac aatcctcgtt ttcttgctat gcaaatagaa caaatgtatg
      241 aaattgtagt acctgaattt gttgaacccg ttttagaaca aggtatcaag gataagtctt
      301 tggagattga aaattcacat gaaattgcag aagttatcat ggtattaagt aatgtatggc
      361 tcaacccatt agttgaaatg acggataaag agggtatgga aaaacgttgc aatacattca
      421 atgccctttt aaatggaata ggcatagaca cattattaga cgaagaaaca atttctgcat
      481 ttatacagtt ttgtaaataa ctgtatttgt tatccctata cgatagactg ctctataaaa
      541 gagcagttta tttaaaccat tatataaacc gacggtctgt ttgattaaat aaaaaggaga
      601 gattttatgc aagcagaaaa aaaacttttt aatcgtgatt ttatacttgt tgttatagga
      661 cagataattt ctttatttgg aaacgctatc ctacgatttg cattaccgta ctatttgtta
      721 aaagaaactg gttcatcagc tttgtttggt attgctacag cctgctcttt tttacctatg
      781 gttattttgt cgctggtagg tggtattatt gctgacagga caaataaaag aaacattatg
      841 gtcatactag attttgtaac agcaatcata attttagtat tttatttgtc attgggaaaa
      901 attcctatca tacctttgtt tattgttgct ttgatgttac tttatggtat ttctggtgca
      961 tatcaaccag cggtacaagc aagtattcct ctattggtat catcagataa acttaccgtc
     1021 ggtaatgcta taataaatca agtaaatact ttagcaaact ttatcggacc agctataagc
     1081 ggagtagttt ttcaatttta tggtatcaat cccattttaa ttgttagtat tatatgtttt
     1141 actttttcag ccattatgga aatttttatc catattccac accaaaaaag acctcgtgaa
     1201 gtaggtgtaa ttgcagttgc taaaaacgat ttaaaagata gttatacctt tttgaaaaag
     1261 gagaaaccca tttttatatc cgtaatcttt ttggtttgca ttttcaattt ggttttaagt
     1321 gctgttttaa ttgttggtct gcctatctta atttctgaag ttttaaaaat gccagataca
     1381 ctatatggtt tttcacaatc agcattatca ttaggtggtt tatgtggtgg tattttaaca
     1441 gctcttgttg cttcaaagtt aaaactaaaa aacctttata ccttgttact tatttgttct
     1501 gtttctgttt tacttatggg aatttcgcta tttttagaat tgcctgcaat agtttcctac
     1561 tgggtagtta cagtgatgtg ctttgtggct atgggtgtat ctactatgtt tgttgttcaa
     1621 atttacactc ttgttcagac acaaacacca ccgcaattag taggaaagat aatggctacg
     1681 ctggtagcag ttgctatgtg cggtcaacct attggacaaa tggtttacgg tattttattc
     1741 gatgtattta tacaagaaac atgggttgtt atgtttgtag cttctattgc ttcaatcatg
     1801 atttcaatat attcaaaaag aatttttcaa aagttagaga tataatacaa ggggttaaat
     1861 agtatgttag gctaaaaaca atatataaaa gaaaatttga aatctgctaa aaggatagtg
     1921 ttataaaaga aagagtaata ttgcagtatt taaggctcgc acaaatatat gttttgtgtg
     1981 ggcttttttc ttaccatttt tctgtatttc taaagggtat aaacggtatt ccgtttatat
     2041 atttcagcat tagcagaaag ggggtgagat tatgaggaca tcttctttcg agcatatcgt
     2101 cagattacag ttcaattctt taatgctgat tgtcattaag aacaaagtga aaagtcgaaa
     2161 cagacagctt gcaagacatt ctaaacgtga agtattgttt tgtgaaatag ccgaaacgaa
     2221 acaagttgaa cgtggaacga ttgatacctg tttttatgat tgtgtatcat ttaaagtctt
     2281 gcactttaca atttatgtat ctgatgaaac acttggtaca gttttacata gactgtcaga
     2341 aaaacagcgt agtgcgattt tattacgtta ttttcaagga atgaacgaca gagaaatttc
     2401 agaactgtac catgtatcac gttctgctat ttgcagtaga agaactagag gactaaggaa
     2461 gttaaaaatg cttttgagcg aaaggaagta aagaaatgag taaagattat ggctatccct
     2521 cttttgaatt gatttgcaaa gcgtcaagcg gagatgaaat ggcagttaaa gagattttga
     2581 agttttatga cggttatatt tctaaattat gcttgcgtcc tttttaccac tctgaaagtg
     2641 gtaaaatcat tatgcaggtt gatgaagaac taaaaggaga aattcataca gacatgatga
     2701 aagcaatatt gaaatttgaa atcggagtga agtaatcaac tgtataaaaa aatcgggaga
     2761 tacacagttt cgtgctatct cccttagtag ttttacggtc atatcaaggc tcattcaatc
     2821 gtggtaaatt cgtggtaaga tgagtgtttt tctatacttc aaaatgcttg aatgtatagt
     2881 gtttatgcgg ataattgaaa attagatata aaatttcaaa taaattactg atataaattt
     2941 acaataagaa ctcttgtttc tacttttttt tatgagaaaa tttagttttc aatttattga
     3001 accattttcg gcttggatct
//
LOCUS       DAZAID010000273          873 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.guided.231, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000273 DAZAID010000000
VERSION     DAZAID010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.guided.231"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            260..808
                     /gene="aac(6')"
                     /locus_tag="ACG9BE_003084"
     CDS             260..808
                     /gene="aac(6')"
                     /locus_tag="ACG9BE_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN3033733.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          311 a          128 c          183 g          251 t
ORIGIN      
        1 ttgtcaattc ataattggtt ttttttaaag caaaagataa tctatatcat gatgaacaaa
       61 cagagttttt tcctccaatt gatggctttc ctgatctttc ctattttgaa taaagtgttt
      121 ctgtataatt ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg
      181 tagctaaaga tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca
      241 agaacaaggg ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa
      301 agatcagctt tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc
      361 agaagaagta gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga
      421 tgagttagta ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca
      481 tccattagtt gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta
      541 cttagaaaaa gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt
      601 agaccatgga acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc
      661 ttccatccag aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat
      721 cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac
      781 gattattcct cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt
      841 caagaaataa gaaggaattt acgaaaatta ctt
//
LOCUS       DAZAID010000274          660 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7117
            SAMEA115708818-rid22583533.guided.245, whole genome shotgun
            sequence.
ACCESSION   DAZAID010000274 DAZAID010000000
VERSION     DAZAID010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708818
            Sequence Read Archive: ERR13198538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288560.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288560.1
            Genome Coverage       :: 123x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:02:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,027
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 33 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 28 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708818-rid22583533.guided.245"
                     /isolate="VRE7117"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..431
                     /locus_tag="ACG9BE_003085"
                     /pseudo
     CDS             82..431
                     /locus_tag="ACG9BE_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT          214 a          122 c          101 g          223 t
ORIGIN      
        1 ttcttatatt tcctaataga gtataacata gctttaagca ttacttaaag ataacttaaa
       61 agttatcttt taaatactta attgttaaat accttatttg atgcaaaaga aatctacgat
      121 agtcaacaaa aaaattttca ttatatccag aaaaattttt cttagttaag gacctatgga
      181 ttgcagtaat gcagaattcg tcgaataaac tgccaaagac atacaatcat attgctttta
      241 aaattgacga acaagatata gattcgtttg tctcaaaaat ccaaatgtta ggtttaactg
      301 tcgaaccagg acgttctcgt gtgaaaggcg aggcttcttc aatttacttt tacgactatg
      361 ataatcacct cttcgagtta cataccggta ctctccagga acgtttaaaa tcctacaatt
      421 ccactacctg agactagtaa tgactagtct ctttttatgg cctctgatcc ccccattgag
      481 cgaatactaa tagggcttcg aattgatctg ttcaatcttt aagcaatatc cctcaaatga
      541 gtttggaaag tttagtgatt catcacaaat acttaaatac atgtagttat atatagattg
      601 aggttcttgt gagcgtatgc tcgtgtgggt tcaaatccca tcgtctgcat ccatctttcc
//
